LOCUS       DASULI010000001         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_408614, whole
            genome shotgun sequence.
ACCESSION   DASULI010000001 DASULI010000000
VERSION     DASULI010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408614"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1260
                     /gene="nagA"
                     /locus_tag="VFX34_00005"
     CDS             82..1260
                     /gene="nagA"
                     /locus_tag="VFX34_00005"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX5563310.1"
                     /translation="MIREVTALMSLLIQNIQIAMPDGSVMKGEIIVENGKISSVSNVS
                     IKDFNGERMDGQGCLVLPGFIDIHIHGADGADFMDGTQDSFKTIADALPKEGTTAFLA
                     TTLTQAEEKIAKAVNAGKGFMERNESGAEMLGFHLEGPFIHPEQAGAQPVEFILKPSL
                     ALLERWFGKDLGHLKVVTLAPECDEGLEMTRQLAEHGILVSAGHTKASFVDITQAIDH
                     GLTHLTHFGNAMSGLHHREIGTVGAGLLLEQLHCEVIADGIHLSEDMLRLIYRTMEPE
                     RIILITDSMRAKGMPDGAYTLAGQDVNVVGAKATLSDGTLAGSVLKMDEAVRMMRSMG
                     AGLPDLIRMSSSNAAKRLGVFDRKGSIEVGKDADLVLLDADFNVHATLCRGMVSYSLE
                     "
     gene            1305..2402
                     /gene="ugpC"
                     /locus_tag="VFX34_00010"
     CDS             1305..2402
                     /gene="ugpC"
                     /locus_tag="VFX34_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019376155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX5563311.1"
                     /translation="MAELKLTNLTKEYDKGVVAVKDFNLDIEDKEFIVFVGPSGCGKS
                     TTLRMIAGLEDITDGDFEIDGKRMNDVAPKNRDIAMVFQNYALYPHMTVYENMAFSLK
                     LRKVKKKEIDEKVQEAAKILGLEDYLDRKPKALSGGQRQRVALGRAIVRDAKIFLMDE
                     PLSNLDAKLRVQMRAEIQKLHRRLRTTTIYVTHDQTEAMTMATRLVVMKDGYIQQVGT
                     PKEVYDFPRNVFVGGFIGSPAMNFFKGTMTEEGFAIGDTNVFIPKGKREMLQGKGYAG
                     KELILGVRPEDIYTKASDIESNGQATFHATIEVAELMGAETYLYASLNGSDFIARIDS
                     DTTIRPSDTIPLAFDMEKAHFFDTETEERIY"
BASE COUNT          772 a          595 c          775 g          657 t
ORIGIN      
        1 cgatacgacg atcacgtatt tggatgcgac gcgcgaagcg atcgatgaca tacggaacga
       61 aacattcaag aagcggagaa aatgatccgg gaggtgacgg cgttgatgtc attgctcatt
      121 caaaatatac aaatcgccat gcctgacggt agtgtgatga agggtgaaat cattgtggag
      181 aacggaaaga tttcatctgt ttctaatgta tctattaaag atttcaatgg tgaacggatg
      241 gacggccaag gctgtctcgt cctcccagga tttatcgata tccatatcca tggcgcagat
      301 ggtgcggatt tcatggatgg cacacaggat tcattcaaaa cgattgcaga cgccttgccg
      361 aaggaaggga cgactgcatt cctcgcgacg acgctgacgc aagccgaaga aaagatcgca
      421 aaagccgtca acgcaggtaa aggttttatg gaacggaatg aatccggtgc cgaaatgctt
      481 ggatttcatc tggaaggccc tttcattcat ccggaacaag caggtgcaca gccggtcgaa
      541 tttatcctga aaccgtcatt ggcgcttctg gaaagatggt tcggaaaaga tcttggacat
      601 ctgaaggtcg tgacgctcgc cccggaatgc gatgagggac tggagatgac acggcagctc
      661 gcagaacatg gcattctcgt ttcggcaggc catacgaaag cgtctttcgt agacatcaca
      721 caggcgatcg atcacggtct gacacatctt acccatttcg gcaatgcgat gagcggcttg
      781 catcatcggg aaatcgggac ggtcggcgcc ggcttattgc tggagcagct ccattgtgaa
      841 gtgattgcgg atgggatcca tttgtcggag gatatgctgc ggctcattta ccggaccatg
      901 gaacccgaac ggatcatcct cattacggat tcgatgcggg cgaagggtat gccggatggg
      961 gcatatacat tggcgggcca ggacgtgaac gtggttggtg cgaaggcgac actttcggat
     1021 ggaacattgg cgggaagcgt tttgaaaatg gatgaagcgg ttcgaatgat gcggtccatg
     1081 ggagcgggcc tgcccgactt gatacgaatg tcttcctcca atgcggcgaa acggttaggc
     1141 gtgttcgacc gaaaaggtag catcgaagtc ggaaaagatg ccgatcttgt gctcttggac
     1201 gccgacttta acgtgcatgc aaccctttgc agagggatgg tgagctattc cctggaatga
     1261 agtaggagtt tgaagtcata gaaggaattg aggggggaga atggatggct gaactgaaat
     1321 tgaccaattt gacgaaggaa tatgacaaag gcgttgttgc cgtcaaagat ttcaatttag
     1381 atatagagga taaggaattc atcgtgttcg tcggtccttc cggttgcggg aaatcgacaa
     1441 cattgcgaat gatcgctgga ttggaagata taacggatgg cgattttgaa atcgatggca
     1501 agcggatgaa tgatgtcgct ccgaaaaatc gcgatatcgc gatggtgttc caaaactatg
     1561 cactctatcc gcatatgacc gtctatgaaa acatggcatt cagtttgaag cttcgaaaag
     1621 tgaagaagaa ggagatcgat gagaaagtcc aggaagccgc caagatatta ggattggaag
     1681 actatttgga ccgcaagccg aaagcgttgt caggcgggca gcgtcaacgt gtcgcgctcg
     1741 gccgggcaat tgtgcgggat gcaaagatct tcctcatgga tgaaccgctt tccaatttgg
     1801 atgctaagct ccgcgtgcaa atgcgcgctg aaatccagaa gctgcatcgg cgtcttcgga
     1861 cgacgacgat ctacgtgaca cacgaccaga cggaagcgat gacgatggcg acccgtctcg
     1921 tcgtcatgaa ggacggctat atccaacagg tcggtacgcc gaaagaagtg tacgattttc
     1981 ctagaaatgt atttgtcggc ggcttcatcg gttcaccggc gatgaacttc ttcaaaggga
     2041 caatgacgga agagggcttt gccataggag atacgaacgt tttcattcct aaagggaaac
     2101 gtgaaatgct ccaagggaaa gggtatgccg gtaaggaact aatccttggt gtacgaccgg
     2161 aggatatcta cacgaaagcg tctgacatcg aatcgaatgg gcaggcaact ttccacgcga
     2221 caatcgaagt ggcggaactg atgggggcgg aaacgtattt atatgcatcc ttgaatggct
     2281 ccgactttat cgcacggatc gattccgaca cgacaatccg tccatcggac acgattccgt
     2341 tagcattcga catggaaaaa gcgcatttct tcgacactga aacggaagag cgcatttatt
     2401 aagttggaaa agggttgctc tcatgatgag aagcaaccct ttgatttgta tagtaacttt
     2461 tagtgttgaa gtttatgtcc atctaaagat ccgtgatccg gattctattg aaaatgatca
     2521 atgccgcggt cattttccac tgggtgtctt ctatccttaa gcaacgcaag ccctaccgac
     2581 tcttgcgtcc ttgcgaccct ttagccgcgc cagtgtgaat aagtgtcctc aaaaacagta
     2641 cgtagaatgc catgtcaaat taaaagttac tatggcgtta actgattgat tataacatgg
     2701 atagaggagc atggcaagtg gcggaaaatg tagatagctt caaaaatatt tcccgggaat
     2761 taaacaatct tcgacagaac ctgcttcatt tacatgaaa
//
LOCUS       DASULI010000002         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1712253,
            whole genome shotgun sequence.
ACCESSION   DASULI010000002 DASULI010000000
VERSION     DASULI010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1712253"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFX34_00015"
     CDS             complement(<1..344)
                     /locus_tag="VFX34_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5563312.1"
                     /translation="MTRIGTLWRKSVDGRTAALYLLPSIILFSVFVFYPMFRTIYLSF
                     FLTDQTGNAAIMVGFENFQYLLGSSEFQNSMKATGLFVLYTVPITIILALFFALLANE
                     KLKGIGFFRTMYS"
     gene            complement(341..1471)
                     /locus_tag="VFX34_00020"
     CDS             complement(341..1471)
                     /locus_tag="VFX34_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563313.1"
                     /translation="MKRVELIDVSKSYDKQVDVISNINVTIEPGEFFVLVGPSGCGKS
                     TMLRMIAGLEEITGGTLKIGEKVANNLSPDKRDLSMVFQNYALYPHLSVQQNITFGLD
                     VKRMKKEDQRKKAIEVAEMLGLTEYLDRKPRELSGGQRQRVALARAIVSETPICLMDE
                     PLSNLDAKLRAHMRSEIRRIQRNLGITMIYVTHDQVEAMTMGDRIMILHEGKIQQIGK
                     PIDIYNYPANPFVATFIGSPPMNLAKAEFDPKTSELVIGNALRLSVPKDEIGKLPRTS
                     MTVGVRPENLHPATPETQEDVFLVEVTNVEILGNETVFSFKLGEEEWMAKWSGQWHID
                     IGDKIPVRMEFESLSIFEEHSGELIKKTIDYGNYVKQREVSP"
     gene            1608..>2257
                     /locus_tag="VFX34_00025"
     CDS             1608..>2257
                     /locus_tag="VFX34_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5563314.1"
                     /translation="MKKGILLSLLILLSACSTASVAATLPADKFLVIAHRGASAYAPE
                     HSLLSYGLAVQMKADYIELDVHMTKDGELVSMHDPFVHLPDGRKRIAEVDFQELKKVH
                     RTSDYDGKLPVSLPEGIDPLRIVSTEEIFSYFQDDVNYYIELKSPTAYPGIEDALLGQ
                     LREFGLLPLEDTELPKVILQSFDAASLKKIHDQEPAIPLIQLYSAKNETMLTKKRL"
BASE COUNT          552 a          559 c          535 g          611 t
ORIGIN      
        1 gaatacatcg tccgaaaaaa tccgatgccc ttcaattttt cattggcaag caacgcgaag
       61 aatagcgcca ggatgattgt gatcggaacg gtatacaaga cgaatagtcc cgtcgctttc
      121 atgctgtttt ggaattcgga agatccgagc aagtattgaa agttttcaaa tccgaccatg
      181 atggcggcgt tgcctgtttg atccgtcagg aaaaagctta aatagatcgt ccggaacatc
      241 ggatagaata caaaaacgga aaatagaatg atagagggca gtaaataaag ggcagccgtc
      301 cggccgtcca cggacttccg ccaaagagtt ccgatgcgtg tcatggcgac acctctcgct
      361 gcttgacata attgccgtag tcgatcgtct ttttgatgag ctctccgcta tgctcttcga
      421 agatggacaa ggactcgaat tccattcgga caggtatctt atcgccgatg tcgatatgcc
      481 attggccgct ccatttggcc atccattcct cctcgccgag tttaaaagaa aagacggttt
      541 cattcccaag gatttcgacg ttcgtcactt ccacaaggaa aacgtcttct tgagtttcag
      601 gagtcgcagg gtgcaggttt tcgggccgta ccccgactgt catcgatgtc cttgggagtt
      661 ttccgatttc gtcctttggt acggataacc gcaatgcatt gccgatgacg agttccgatg
      721 ttttcggatc gaactccgct ttcgccaggt tcatcggcgg cgatccgatg aacgtcgcga
      781 cgaatggatt cgccggataa ttgtatatat cgatcggctt gccgatttgc tggatcttcc
      841 cttcatggag gatcatgatc cgatcgccca tcgtcatcgc ttccacttgg tcgtgcgtca
      901 cgtaaatcat cgtaatgcca agatttcttt gaatccttct gatttccgaa cgcatatggg
      961 cacgcagttt ggcatcaaga ttcgaaaggg gctcatccat caggcagatc ggcgtctcac
     1021 tgacgatcgc cctcgcgagt gcgacccgtt gacgctgccc gccggaaagt tcacgcggct
     1081 tgcggtcgag gtattccgtc aagcctaaca tttcagccac ttcgattgcc tttttccgtt
     1141 ggtcctcctt tttcattctt tttacatcaa ggccgaacgt tatattttgc tgaaccgaca
     1201 aatgcggata cagtgcatag ttttgaaaga ccatggaaag atcgcgttta tccggagaaa
     1261 ggttattggc gaccttctcc ccgatcttca atgtcccgcc ggtgatttcc tcaaggcccg
     1321 caatcatccg gagcatcgtg cttttcccac aaccggaagg accgacaagg acaaaaaact
     1381 cgcccggctc aattgttacg tttatattgg atataacatc cacttgttta tcgtatgatt
     1441 tggagacgtc aatcagttca actcttttca ttgatcaaat tcctttctcc ccatttttat
     1501 attaatctca atgaatgttc tagtttcaca cttcgataaa agatgtcaag attccttcct
     1561 tttttctcaa tctttacaat tactttacgt ttaaggggga aacaaccgtg aaaaaaggga
     1621 ttcttctaag cctgctcatc ctgctatcgg catgctcgac cgcttcggtt gcggcaactt
     1681 tgccggctga caagttcttg gtaatcgccc atcgcggagc ttccgcctat gcgccggagc
     1741 actctttgct atcttatgga ttggcggtgc agatgaaggc ggattatatc gaactggatg
     1801 tgcacatgac gaaagatggg gaactcgttt cgatgcatga cccgttcgtt catttgcctg
     1861 acggcagaaa acggatagcg gaagtggatt tccaggaact gaagaaagtt caccggacgt
     1921 ccgactatga cgggaagctg ccggtttcat tgccggaagg catcgatccg ctgcgcattg
     1981 tcagtacgga ggagattttt tcgtatttcc aagacgacgt caattactat attgaactga
     2041 aatcacccac cgcctacccg ggaattgaag acgctttgct cggacagctg agggagttcg
     2101 ggctgctgcc gttggaagac acggaattgc cgaaagtcat cctgcaatca ttcgatgccg
     2161 cctctttgaa gaaaatccat gatcaggagc cggccattcc gcttatacaa ctgtattccg
     2221 cgaaaaatga aacgatgcta acgaaaaagc gtttgga
//
LOCUS       DASULI010000003         6321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_777742, whole
            genome shotgun sequence.
ACCESSION   DASULI010000003 DASULI010000000
VERSION     DASULI010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777742"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1957
                     /gene="gyrB"
                     /locus_tag="VFX34_00030"
     CDS             35..1957
                     /gene="gyrB"
                     /locus_tag="VFX34_00030"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX5563315.1"
                     /translation="MEEKELQTAYDANQIQVLEGLEAVRKRPGMYIGTTSSRGLHHLV
                     WEIVDNSIDEALAGYCDHIEVTIEKDNWIRVDDNGRGIPVGIQESTGRPAVEVIMTVL
                     HAGGKFGGGGYKVSGGLHGVGASVVNALSEATEVFVRRDGKVHFIRFERGILSHELEV
                     IGDTDETGTRTRFKADPEIFTETTVYEYEILAHRLRELAYLNRGLRISITDEREEEIR
                     TDTFYYEGGIKSYVEHLNKNKEPIHEEPIFIEGEKDGISIEIAMQYNSGYAENLFSFA
                     NNINTYEGGTHESGFKTALTRVVNDYARKNGMLKEADPNLSGDDVREGLTAIISIKHP
                     DPQFEGQTKTKLGNSEVSTITNALFSEGFQRFLLENPTTARLVIDKSLMAARARIAAR
                     NARELTRRKSALEVSSLPGKLADCSSRNPEISELYIVEGDSAGGSAKSGRDRHFQAIL
                     PLRGKILNVEKARLDRILGNAEIRMMITALGTGIGDEFDLSKARYHKIVIMTDADVDG
                     AHIRTLLLTFFFRFMRPLIEAGYVYIAQPPLYRVKQGKHEEYCFDEQELQEILNRLSS
                     VPKPVITRYKGLGEMDATQLWETTMDPDQRTLLQVHLEDAFDADATFEQLMGDEVEPR
                     RRFIEENAMYVKNLDA"
     gene            2065..4602
                     /gene="gyrA"
                     /locus_tag="VFX34_00035"
     CDS             2065..4602
                     /gene="gyrA"
                     /locus_tag="VFX34_00035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5563316.1"
                     /translation="MADMPKRGVQGINISTEMKTSFLDYAMSVIVSRALPDVRDGLKP
                     VHRRILYAMQDLGNTADKPHKKSARIVGDVIGKYHPHGDSAVYDTMVRMAQDFNYRYM
                     LVDGHGNFGSVDGDAAAAMRYTESRMSRIAMELLRDINKDTIDYQDNYDGQEKEPIVL
                     PSRFPNLLVNGTSGIAVGMATNIPPHHLGETIDGVLAMADNPAISTEELMEIIPGPDF
                     PTGGIILGRSGIRRAYETGRGSIIIRGKVEIEQQSNGKETIIVTELPYQVNKARLIEK
                     IAELVRDKRIEGITHLADESDRTGMRIVIEVRRDANSHVLLNNLYKHTALQSSFGINM
                     LALVDGQPKILSLKEILYHYLEHQKVVIRRRTQYELNKAEDRAHILEGLRIALDHIDE
                     IIALIRGSKTTDEAKTGLMERFNLSERQSQAILDMRLQRLTGLERDKIENEYQELLVL
                     ISELRAILADEEKVLDIIREELLEVKQRFADDRRTEIGSGGAEMLEDEDLIPVENSVL
                     TLTHNGYIKRLPASTYRSQRRGGRGIQGMGTNDDDFVEHLLNTSTHDTILFFTSRGRV
                     FRKKGYEVPEFSRTAKGLPIINLLDVDKDEKVTAMIPVDKFEEDKYLFFATRGGVVKR
                     TSVADFANIRSKGLIALTLRGDDELIGVKMTNGEQNIAIGTRDGMLIKFDERDIRSMG
                     RVASGVRGIRLREGDIAVGMDTVSEGDEILVVTEKGYGKRTPEEEYRIQSRGGYGLKT
                     LNVTERNGQLVAMKTVDGSEDLMLITIHGILIRMDIDDISSIGRSTQGVRLIRLGEDE
                     LVATVAKVEKEEEHEETDDHEDTKEVNTDVEMDDSIEADDAVETEEE"
     gene            4722..5819
                     /locus_tag="VFX34_00040"
     CDS             4722..5819
                     /locus_tag="VFX34_00040"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5563317.1"
                     /translation="MEVYKTISELRAGISLKEDVFGKTKYPIMRKDTELTLEHIDVLQ
                     AFGVKRVKVEERVTQKDIPETEKNSSIDAEDLLDNLPLSVYQLRKEYNEAVRSYKKEF
                     SYWRAGQRPDVPKVRGFIIPLLEKFIKQKQMLTMLNDFSNPEEYIYHHSVAVGILAAA
                     ISSQMGYEKGDALQLGLAGALADCGMAKISPAILEKTAFLTKEEYNEVKKHTLFSVKM
                     IQDTPLLRQDMKLAILQHHERLDGSGYPRGEKMDTISVFSQILAVADVYHAMTSERLY
                     RLRHSPYKVIEMIKEEEFGKFDIKVVEALHRLVGNLSIGTRVKLTDGEVGEIMFIHRD
                     TPLRPMIKKEADGTMIDLATHRSIAILKIIE"
     gene            6178..>6321
                     /locus_tag="VFX34_00045"
     rRNA            6178..>6321
                     /locus_tag="VFX34_00045"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1832 a         1357 c         1690 g         1442 t
ORIGIN      
        1 tgatgttcgg aaaggaaagg tggacatttt ggctatggaa gaaaaagaat tgcagacagc
       61 ttatgatgcg aatcagatac aggtccttga aggcttggaa gctgtccgta aacgtccggg
      121 catgtacatt ggtacaacga gttcgcgggg gctgcaccac ctcgtatggg aaattgtcga
      181 caatagtatc gatgaggcgc ttgcgggcta ttgcgatcat attgaagtga cgattgaaaa
      241 ggataactgg atccgtgtag acgataacgg acgcggcatt ccagtaggca tccaggaatc
      301 gacaggcaga cctgctgttg aagtcataat gacggtcctt cacgcagggg gcaaattcgg
      361 aggcggaggc tacaaagttt caggcggatt gcacggggtg ggcgcttctg tcgtgaacgc
      421 actatccgag gcgacggaag tgtttgtacg acgggatggc aaagttcatt tcatcaggtt
      481 tgaacggggc atcctatcac atgaattgga agtcatcgga gataccgacg aaaccggcac
      541 acgtacacga ttcaaagccg atccggaaat cttcacggaa acgactgtct atgaatatga
      601 aatactggca catcgtcttc gtgagctggc gtaccttaac aggggcttgc gcatcagcat
      661 cacagatgaa cgtgaagagg aaatccgtac agacacgttc tactacgagg gcggcatcaa
      721 atcctacgtt gagcatttga ataaaaacaa agagccgatt cacgaggaac cgattttcat
      781 agaaggcgag aaagatggca tctccatcga aatcgcaatg caatataata gcggctatgc
      841 ggaaaacctg ttttcattcg cgaataacat caatacgtat gaaggcggta cccatgaatc
      901 aggtttcaag accgctttga cacgtgtcgt caacgactat gccaggaaga acggaatgct
      961 gaaagaagcg gatccgaacc tttcgggcga tgatgtgcgt gaaggattga cggcaatcat
     1021 ttcaatcaaa cacccggatc cacagttcga aggacagacg aaaacgaaat tgggcaactc
     1081 cgaagtaagc acgattacaa atgcgctatt ttcggaaggg ttccaacgtt tccttcttga
     1141 aaacccgaca actgcacgat tggtaatcga taagagcctc atggctgcac gggcgcggat
     1201 tgccgccagg aatgcacgtg aactaacacg ccggaaatca gcattggaag tatcaagcct
     1261 tcctggtaaa ttggcggact gttcttctcg taatccggaa atcagcgaat tgtatatcgt
     1321 tgaaggagac tcggcgggcg gttccgcgaa aagcggacgt gaccgtcatt tccaggcaat
     1381 cctgccattg cgcggtaaaa tcttgaacgt tgaaaaagcc agattggacc gtattttagg
     1441 aaatgcggaa atcagaatga tgattacggc actcggtaca ggcatcggag atgaattcga
     1501 tctttcgaaa gcgcgttacc ataaaattgt catcatgacg gatgccgatg tggatggagc
     1561 gcatatccgg accctgctat tgacattctt cttccgtttc atgcgtccgt tgatcgaagc
     1621 gggctatgtc tatatcgcac aaccgccgct ttaccgggtc aaacaaggca agcatgagga
     1681 atactgtttc gacgaacaag agttgcagga aatcctgaac cgactttcat ccgttccgaa
     1741 gccggtcatt acccgctata aaggtctcgg tgaaatggac gccacccagt tatgggagac
     1801 gacgatggat ccggatcagc gtacactcct gcaagtgcat ctcgaggacg ctttcgatgc
     1861 agatgcgaca ttcgagcaat tgatgggtga cgaagttgaa cctcgacgca gattcatcga
     1921 agaaaatgca atgtatgtaa agaacttgga tgcgtaatcg atttattcaa taaatgaaaa
     1981 tgaacaggga acctggcaga tggggctttg ttcagttgac aggctgtgcg tttaaagcgc
     2041 ctgagttgaa aggagtatga caatatggca gatatgccaa aacgtggtgt ccaaggcatt
     2101 aacatcagta cggagatgaa gacatctttt ctcgattatg cgatgagtgt catcgtctca
     2161 agggcattgc ctgacgtgag ggatgggttg aagcccgtcc accgccgtat tctttatgcg
     2221 atgcaggatc ttgggaacac cgcggataaa ccacataaga aatcggcccg tatcgtcggg
     2281 gacgtaatcg gtaagtacca cccccatggc gatagtgccg tctatgacac gatggtcaga
     2341 atggcgcagg atttcaacta tcgttatatg ctcgtcgatg gccacgggaa cttcggttct
     2401 gtcgatggcg atgcagctgc agcgatgcgt tatacggaat cgagaatgtc ccggatcgca
     2461 atggaattat tgcgtgacat caataaagac acgattgatt accaagataa ctatgatggc
     2521 caagagaaag aaccgatcgt cctgccgagc cgttttccga atctgcttgt gaacgggaca
     2581 tcgggtatcg cggtcggaat ggctacgaac atccctcctc accatttggg tgaaacgatt
     2641 gacggcgtgc ttgcaatggc ggataatcca gcaatttcga ccgaggaatt gatggaaatc
     2701 attccaggcc ctgatttccc gacaggcggg atcatcctcg gaagaagcgg aatccggcgg
     2761 gcttatgaaa ccgggcgcgg gtcgattatc atcagaggca aagttgaaat tgagcagcag
     2821 tcgaacggca aggaaacgat catcgtaacg gagctgcctt atcaggtgaa caaggcacgc
     2881 ctcattgaaa agatcgccga gcttgtccgg gacaaacgta tcgaaggcat cacgcatttg
     2941 gcagatgaat ccgacaggac gggtatgcgt attgtcatcg aagtccgcag ggatgcaaat
     3001 tcacacgtat tgttgaataa cctttacaag catacggccc tccaatcgag cttcggcatc
     3061 aatatgcttg cgctcgtgga tggacagccg aaaatcttgt cactgaaaga aatcctttat
     3121 cattatttgg aacatcagaa agtggttatc cgcagacgta ctcaatacga gttgaacaaa
     3181 gcggaagacc gtgcgcacat cttggaaggt ttgcggattg cacttgatca cattgatgaa
     3241 atcatcgctc tgatccgcgg ttcgaaaacc accgatgaag cgaaaacagg tctgatggaa
     3301 cgtttcaatt tatccgaaag gcaatcccaa gcgatcctcg acatgcgtct tcaacgtttg
     3361 acaggattgg aacgtgacaa aatcgagaac gagtaccaag aactcttggt attgatttcc
     3421 gagttgcgcg ccattctggc tgacgaggaa aaagtgctcg atatcatacg tgaggagctg
     3481 cttgaagtga agcagcgttt cgccgatgac cgcaggactg aaattggaag cggcggggcg
     3541 gaaatgctcg aggatgaaga cctcatccca gttgaaaact ccgtattgac attgacgcat
     3601 aacggctaca tcaaacggct tcctgccagc acgtaccgga gccagcgtcg tggaggacgc
     3661 ggaattcaag gcatggggac gaatgacgat gactttgtcg agcatctgtt gaacacatcg
     3721 acacacgata cgattctgtt cttcacgagc cgtggtaggg tattccgtaa aaagggatat
     3781 gaagtaccgg aattcagccg gacggcgaaa gggctgccga tcatcaactt gttggatgtc
     3841 gataaggatg aaaaggtaac ggccatgatt cctgtggata aatttgaaga ggataaatat
     3901 ctcttcttcg caacgcgggg cggcgtcgtc aagcgtacgt cggtcgcaga ctttgccaat
     3961 atccgttcca aggggttgat cgctttgaca ttgcgcggcg atgatgaatt gattggcgtg
     4021 aaaatgacga acggcgaaca aaacattgca atcggtacac gtgatggcat gctgatcaaa
     4081 ttcgacgagc gtgatatccg ttccatgggc cgtgtcgcaa gcggcgtgcg cggaatccgg
     4141 ttacgtgaag gggatatcgc ggtcggcatg gataccgttt cggaaggcga tgaaatcctg
     4201 gtcgttacgg aaaagggcta cggaaaacgg accccagaag aagaataccg catccagtca
     4261 cgtggcggat atgggctgaa gacattgaat gtcaccgagc ggaacggcca gctcgttgct
     4321 atgaaaacag tcgacggatc ggaagacctc atgctcatca caatccatgg catcctcatc
     4381 cgcatggata ttgatgatat ttcctcaatc ggccgcagca cgcaaggcgt acgtctgatc
     4441 cgtctgggag aagacgaact tgtcgcgaca gttgcaaaag tcgagaagga agaagagcat
     4501 gaagagaccg atgatcatga agatactaaa gaagtgaata cagacgtgga gatggatgat
     4561 tccatcgaag cggatgatgc agtggaaaca gaagaagaat gatgtaaaaa agttattggg
     4621 acggcaaaat gccgctcaat aacttttttt ctatcgtcta ttttgaatat ttgcagtata
     4681 atagtcctta agagacgatc ggaaagtggt gacaccgtag aatggaagta tacaaaacga
     4741 tcagtgagct gcgtgcagga atttccctta aagaagatgt ttttggcaag acgaagtatc
     4801 cgattatgcg aaaagatacc gaacttacat tggaacatat tgatgtattg caggcttttg
     4861 gagtgaaacg agtgaaggtg gaagagcggg tcacccaaaa agacattcca gaaaccgaga
     4921 agaattcttc catagatgct gaagaccttc tggataatct tccgttgagc gtgtaccagc
     4981 tgcgcaaaga gtataacgaa gccgtccgca gttataagaa agaattcagc tattggcggg
     5041 ccggccaacg tcctgacgtc ccgaaagtcc gtggattcat tattccgctg cttgagaaat
     5101 tcattaagca gaagcagatg ctcacaatgt tgaacgattt ttccaatccg gaagaatata
     5161 tttatcatca ttccgtcgct gtcggaatcc ttgcagcggc catcagcagt cagatgggat
     5221 acgaaaaggg agacgctcta caacttgggt tggcgggggc tctagcggat tgcggcatgg
     5281 cgaagatatc tcctgccata ttggaaaaaa ccgcattcct gacgaaagaa gaatacaacg
     5341 aagtaaagaa acatacattg ttcagcgtga agatgattca ggacacgccc ttgctcaggc
     5401 aggatatgaa gcttgcaatc cttcagcatc atgagcgtct cgatggcagc ggctatccgc
     5461 gcggagagaa gatggacacc atttcggttt tctcccaaat ccttgccgtg gcggatgtct
     5521 accatgcgat gacgtccgaa agattgtata gattgagaca ttccccttat aaagtgattg
     5581 aaatgatcaa agaggaagag ttcggcaaat tcgatatcaa agtcgttgag gcacttcata
     5641 ggctcgtcgg caatctttct attggcacaa gagtgaagct gactgatgga gaggtgggcg
     5701 agatcatgtt catccatcgg gacactccat taagaccgat gatcaagaaa gaagcggatg
     5761 gaacaatgat tgatttggcg actcaccgga gcattgcgat attgaagatt attgaatgaa
     5821 tggagccgct gctcggcggc tttattttcc agttaaagag ttgtaaaaag gtattgcaaa
     5881 cataaatgca agctggtata gtaaataacg ttgcatttcc ggtaagtgaa taaccacttt
     5941 aagatagttc aaaacttttt taaaaagatg ttgacatcgg aattgcaaac atggtattat
     6001 ataagagttg cttctgacgg agcaacgcac aatgaacctt gaaaactgaa cagcaaaacg
     6061 tcaacaaata aaagtcttgt agccgacttg tcggcataca ggcaaaacga atcttcggat
     6121 tcgaaattga catcttaaat gatgccagca agaaactcga gctactcgaa tttctctatc
     6181 atggagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc
     6241 gagcggattg aagggagctt gctccctgat attagcggcg gacgggtgag taacacgtgg
     6301 gcaacctgcc ctgcagatgg g
//
LOCUS       DASULI010000004         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1478888,
            whole genome shotgun sequence.
ACCESSION   DASULI010000004 DASULI010000000
VERSION     DASULI010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478888"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VFX34_00050"
     CDS             <1..242
                     /locus_tag="VFX34_00050"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5563318.1"
                     /translation="MLTKKRLEKVAEYASGIGVDKTAVTEELVELVHDVELHVHPFTV
                     NDEKEIRHMIEIGADGIFTDVPDVAVEVLEEMHGD"
     gene            complement(459..1625)
                     /locus_tag="VFX34_00055"
     CDS             complement(459..1625)
                     /locus_tag="VFX34_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5563319.1"
                     /translation="MRNEEMLLIPGPTPVVDSIYDAMASETRGHTDPRFVEIYKRSIE
                     NTREMFKTDGEVFVIAGSGTIGMEMALVNTVGAGERLLVISHGYFGDRFIQLGKAFGI
                     EIDVLQAEWGQQVAPEEVERQLSERTYKAVTITHADTSTGVAADLDALVPIVKKHGAL
                     VILDGVCATAAMEEDMSKEYGGAGYKIDVVLTGSQKAIGVPPGLAIVAFNQTALEARK
                     QLGHIPAYYCDIENWIPIMHDPSKYFATPPVNLIYAYDEGMKLVLEEGLEKRYARHTA
                     YGRGVRSALREFGMKPLAEEEVAASTLSCFLYPEGVNDAEFRAALAGVGLIVAGALAH
                     LGGKAFRLGHMGNTTEEMLVSAIEKIGEALKDVGQDVSIEQAVNRFKEETASIV"
     gene            complement(1758..1883)
                     /locus_tag="VFX34_00060"
     CDS             complement(1758..1883)
                     /locus_tag="VFX34_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfH family protein"
                     /protein_id="HEX5563320.1"
                     /translation="MQKTKEAQKSKKYCRECGCEIVEKVESLLYECERCIGKHKE"
BASE COUNT          498 a          565 c          470 g          538 t
ORIGIN      
        1 cgatgctaac gaaaaagcgt ttggaaaaag tcgcggagta tgcgtcaggc atcggtgtgg
       61 ataaaacggc agtgacggag gaattggtag agcttgtgca cgatgtggaa ttgcatgtcc
      121 atccattcac tgtgaatgat gaaaaagaga tccgccacat gatcgagatt ggtgcggatg
      181 ggattttcac ggatgtgccc gatgtggcgg ttgaggtgtt ggaggaaatg catggagatt
      241 gaggggtttc gcgcttgaat ttcccaggta taaagtcccg agatgagcat aactatgaca
      301 aagtcgcgca taactcgtga aaatttgagc ataactcggt tcaacttgag cataaccagc
      361 ccaaaatcga gcataaccac acccgactcg cgcataactt ctccagtcta gaaaaaatca
      421 cccgattccg ttatggaatc gggtgattct ttcatttatt acacaatcga tgcagtttct
      481 tccttgaaac ggttaacagc ttgttcgatt gaaacgtctt gtccaacatc cttcaatgct
      541 tcgccgatct tttcgattgc tgaaacaagc atttcctctg tcgtgttgcc catatggccg
      601 aggcggaatg ccttgcctcc caagtgcgcc aatgcgccag cgacgatgag tccaacaccc
      661 gccaaagcgg cccggaattc cgcgtcattt acgccttccg gatataggaa gcagctcagt
      721 gtcgatgcgg ctacctcttc ttccgccaac ggcttcatgc cgaattcacg gagtgcggag
      781 cggacgccgc gcccatatgc ggtatgtctc gcatagcgtt tttccaagcc ttcctctaga
      841 accagtttca tgccttcgtc gtatgcatag atcaagttca ccggaggcgt tgcaaaatat
      901 ttggacggat catgcatgat cggaatccag ttttcgatat cgcaataata cgcagggata
      961 tgtccgagct gcttgcgtgc ttccaatgcg gtttgattga acgcgacaat tgccagcccc
     1021 ggcggaacac cgatggcctt ttgggagccc gtcagcacga cgtcgatctt atagccagcg
     1081 ccgccatact ccttgctcat atcctcttcc atcgcggcag ttgcgcaaac tccgtccaaa
     1141 atgacgagcg cgccatgctt tttcacgatt ggaacgaggg catccaagtc tgccgctaca
     1201 ccggtggatg tatccgcatg tgtgatcgtc acagccttgt atgtacgctc cgacagttgg
     1261 cgctccactt cttcaggtgc tacctgctgt ccccattcag cttgaaggac atcgatttca
     1321 attccgaacg ccttcccgag ctggatgaaa cggtccccaa aatagccatg gctgatgacc
     1381 aataaccgct ccccggcacc gaccgtattg acgagcgcca tctccatacc aatcgtcccc
     1441 gacccggcga tgacaaaaac ctctccgtcc gttttgaaca tttcacgcgt attctcgatg
     1501 gaacgtttgt aaatttccac gaagcgcgga tcggtgtgac ctctcgtttc acttgccatc
     1561 gcatcgtaaa tcgagtcgac gacaggcgtc ggtccaggaa ttaaaagcat ttcttcatta
     1621 cgcatatact acatcccctt tacttagatg atcttctcta ttatacccca ttcgccagaa
     1681 taggcatgaa accctcttat tcaaagtaat aaagggaaaa gcccgttccc ccataggcgg
     1741 gagagcgggc gattccttca ctccttatgt ttgccgatgc agcgttcgca ttcatagagc
     1801 agggattcaa ctttctcgac gatttcacag ccgcattccc ggcaatactt tttcgacttt
     1861 tgcgcttcct tcgttttttg catcatgaac cctccttttc ctatgatagt ttttcatagc
     1921 ctggcagcgt cagaaattcc tcaaactctt cttgaagcgt caaatcgacg aacagatccg
     1981 cagcctcgtt gaatttgccg aaacggaact ccttttcccc gacggcttcc ttcaacttct
     2041 tcatctcatc aacaaatacg tccttgaata a
//
LOCUS       DASULI010000005         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1303870,
            whole genome shotgun sequence.
ACCESSION   DASULI010000005 DASULI010000000
VERSION     DASULI010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303870"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFX34_00065"
     CDS             <1..193
                     /locus_tag="VFX34_00065"
                     /inference="COORDINATES: protein motif:HMM:NF021854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HEX5563321.1"
                     /translation="AIQKEFYGMLSADDVELDDLKQKVEEVNGQNAIVQTAITEFNDA
                     TKKVNSLKEDVFSSLEQEE"
     gene            445..1560
                     /gene="pdhA"
                     /locus_tag="VFX34_00070"
     CDS             445..1560
                     /gene="pdhA"
                     /locus_tag="VFX34_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5563322.1"
                     /translation="MAAKKEKQFDPVKTLHSIEEQFEMFQILNEEGEIVNKDADPNLS
                     DEQLTELMTRMVYTRILDQRSISLNRQGRLGFYAPTAGQEASQLASHFALEKEDFILP
                     GYRDVPQLIFHGLPLHMAFLWSRGHYLGSAFPEGLNVLFPQIIIGAQYIQAAGVALGF
                     QKRGIKAVAMTYTGDGGTSQGDFYEGINFAGAYRSPAIFVVQNNQYAISTPRELQTAA
                     KTLAQKGVAAGIPSILVDGMDALAVYVATRDARERAVNGEGPTLIETMCYRYGPHTMA
                     GDDPTRYRTSDTDNEWEKRDPLIRFRKYLEGKGIWNEEKENEVIERAKEDIKEAIKKA
                     DAAPKQKVTDFMKIMYKGDMPYNLQEQFDMYTEKESK"
     gene            1564..>2463
                     /locus_tag="VFX34_00075"
     CDS             1564..>2463
                     /locus_tag="VFX34_00075"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5563323.1"
                     /translation="MAQMTMIQAITDAMRTELKNDENVLVFGEDVGVNGGVFRATEGL
                     QKEFGEERVFDTPLAESGIGGLAIGLTLTGFRPVMEIQFFGFLYEVMDSVSGQLARQS
                     FRTGGKFNAPVTIRSPFGGGVATPEMHADSFEGLVAAQPGLTVVIPSTPYDAKGLLIS
                     AIRDENPVVFLEHMKLYRSFRQEVPEEEYTIPLGKADVKREGTDLSIITYGAMVHESL
                     KAAEALEKEGHSVEVVDLRTVQPLDIETIIGSVEKTNRAIVVQEAQKQAGIAANVVSE
                     ITERAILSLEAPVLRVAAPDTIYP"
BASE COUNT          745 a          498 c          616 g          604 t
ORIGIN      
        1 cgcaatccaa aaggagtttt atggcatgct ttctgccgac gatgtcgaac tggatgactt
       61 gaaacagaaa gtggaggaag tgaatgggca aaatgcaatt gttcaaactg ccatcactga
      121 atttaatgat gcgacgaaaa aagtgaattc cttgaaagaa gatgtatttt caagtttaga
      181 acaagaagaa taaaggaaat gggtaaagtg gagacaatct aggctccact ttttcttttt
      241 tgtgaatcta ttataataca gatgcggtat tgtagtagta cagaattgag ctttattgca
      301 attcagaata caaaagcatt ctcaaacacc atgattaaca gtgattgacc taattgtcct
      361 ctttcagtat actgtttagt gaagatggtt gtatcaatat tggaaacagt aataaataat
      421 acagataaaa ggagagttgc cgatatggct gcaaaaaaag aaaagcagtt cgatccggtg
      481 aaaacgcttc attctattga agaacagttt gaaatgttcc agattttgaa tgaagaaggc
      541 gagattgtaa ataaagatgc ggatccgaac ttatctgatg aacaattgac agaattgatg
      601 acacggatgg tctacacgag aattttggat caacggtcaa tttctttgaa ccgccaaggt
      661 agactcggat tctacgcacc gactgcaggt caggaagcat cccagcttgc atcccatttt
      721 gcgcttgaaa aggaagactt cattctcccg ggatatcgtg atgtcccaca actcatattc
      781 cacggtttac cattacatat ggcgttctta tggtcccggg gacattatct cggaagcgca
      841 ttccctgaag ggttgaacgt tctatttccg caaatcatca tcggcgccca gtatatccag
      901 gcggcaggtg ttgcactcgg attccaaaaa cgcggcatta aagcggttgc catgacttac
      961 acaggcgacg gtggtacttc gcaaggagac ttctacgaag ggatcaactt tgcaggtgca
     1021 tatcgctccc cggcaatttt cgttgttcaa aacaaccaat atgcgatttc gacaccgcgt
     1081 gaactgcaaa cggctgcaaa gacgcttgcg caaaaaggcg tggcggctgg gattccaagc
     1141 atcttagttg atggaatgga cgcgcttgca gtttacgttg caacacgcga tgcaagggaa
     1201 cgtgccgtca acggtgaagg tcctacgttg attgagacaa tgtgctaccg atacggtccg
     1261 catacgatgg ctggagacga tcctacccgt taccgtactt ctgatacgga caatgaatgg
     1321 gaaaaacgcg atccactcat ccgtttccgt aaataccttg aaggcaaagg catttggaac
     1381 gaagagaagg aaaatgaagt gatcgagcgt gcaaaagaag acattaaaga agcgatcaaa
     1441 aaggcggatg ctgcgccgaa gcaaaaagtg acggatttca tgaaaataat gtataaaggc
     1501 gatatgccgt acaatttgca ggagcaattt gatatgtata cagagaagga gtcgaagtaa
     1561 ccgatggcac aaatgacgat gattcaagcg attaccgatg caatgcgcac ggagcttaaa
     1621 aatgatgaaa acgtcctcgt cttcggtgaa gacgttggag tgaacggagg tgtcttccgc
     1681 gcaactgagg ggctgcaaaa agagtttggt gaagagcgtg tattcgatac accgcttgca
     1741 gaatcaggaa tcggcggact tgcgattggg ttgacgttaa caggtttccg tccggtcatg
     1801 gaaatccagt ttttcgggtt cctctatgaa gtgatggact ctgtcagcgg ccagttggca
     1861 cgtcaatctt tccgtacagg cggaaaattc aacgcacccg tgacaatccg ttctccattc
     1921 ggtggcggtg tggcaacacc tgagatgcat gcggacagct ttgaaggact agtcgcagct
     1981 caaccgggat tgactgtagt catcccgtct acgccgtatg atgcaaaggg gttattgatc
     2041 tctgcaatcc gcgatgagaa cccggtcgtt ttcttggagc atatgaaact ttaccgttca
     2101 ttcagacaag aagttcctga agaggaatac acgattcctt taggaaaagc ggatgtgaaa
     2161 cgggaaggta cagacctttc gattattacg tatggtgcaa tggtgcatga aagcttgaag
     2221 gcagctgagg cacttgaaaa agaaggccat tccgtagaag ttgtcgactt acgtacggtt
     2281 cagccgttgg acatcgaaac gatcatcggt tccgtcgaga agacaaaccg tgcaatcgtt
     2341 gtacaagaag cacaaaaaca agcaggtatt gcagcgaatg tcgtatctga aattacagaa
     2401 cgtgctatct taagcttgga agctccagta ctaagagtag cagctccaga tacaatctat
     2461 cca
//
LOCUS       DASULI010000006         5164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1867808,
            whole genome shotgun sequence.
ACCESSION   DASULI010000006 DASULI010000000
VERSION     DASULI010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867808"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4283
                     /locus_tag="VFX34_00080"
     CDS             <1..4283
                     /locus_tag="VFX34_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEX5563324.1"
                     /translation="KMTLSGTVAHGETYVLYHNQAVDEIKDKGNLGNSSVINFNGDDP
                     LVLKKSGTVIDSIGQVGSTAKWGVDVTLVRNAAVTKGDSNPGDAFNRDEEWTVYPKDT
                     FDHLGLHQMDGTPTDPVDPGDPDDPGDISTIGDVRSKALGEIVTIKGTVAAKLKNTIS
                     VQDSTGGIAVRPTSLNVQIGDEVTLTGELADYRGLLQLDKATIVEVKKDAGAPEPIVI
                     TGSEVDEAVESQLVTVHNAILSDVQTGSGWSNYLATDGTGDFVVRDETASLGLTVGTA
                     YDSITGIVQQFDNDYQIIPRSTVDVVVDSSVIQPVVASPGSGTFVGGVKVSLKTGTSG
                     AKILYTLDGTSPIENGATYATPIDIQEDTTVKAVVKTDDDRFGDVFTFTYTITEALQI
                     HDIQGDGHTSPFENQTVEGVEGIVTYKYVLNGSTYYHIQTPDDKRDGNLNTSEAIVLY
                     SGKDAWNLSVGDLVSVTGKVSEYAIDGYADRQETDLKTTQINVRDDQGGKVKILERNV
                     PLPTPFFINEENLPQHEIASHQFAEFNPEKYAADFWESREAMLVQVGNVKAVGPQEHG
                     DLVTVLENAETETLNGGILLKENDKNAERVQFRLEPNGPARDFEVATGDRFAGPITGV
                     VGYSFQNYKIYTSLSDMQQAHSKGEAVPEKTTIVKDADKLTIASYNLENFSNNTKTTS
                     NDKARKLARAFVQDMQSPDIIGVTEVQDNNGPDAGDSRANESYERLIQAIVDAGGVQY
                     EYVNIDPVNNEDGGQPNANIRVGFLYNPARVSLTEGMPKGDATTAAHYENGKLTHNPG
                     RIAPNDPAFNSSRKPLAAQFDFQGESVIAIVNHWNSKTGDTPLFGSQQPPVYGSEAQR
                     KQIAQLVYDFISEIKADNPEANIVSLGDFNDFQFAEPLKIHEGSLMTNMINHVEAADR
                     YSYVHQGNSQVLDHILVSNNLADQTEIDILHINADFTDMAGRASDHDPVMVQIGFEAP
                     VVWEPVPIKKVYNLINEHKKQIMIAEPSVSVYMDAQSSLKDGIYLKGDYAELTGEGFK
                     SNTVILQPKKKGLIVDMKGTEMGHVIVEGSHPLEIRGAENIKRIDFVKGADASEVVFY
                     NSKGKRIGVPTVNNAPVITKPIPNQEAKAGETISIVLSGHFSDPDGDKLTYSATKGTV
                     DLTKNVLTLQLEEGSHIVGVTATDGDKSVTASFSVTVTKAEVPADGYYKHAIGKEGQE
                     LKAALHDIISDHRKLSYAQVWDALKETDKDPNNPNNVILFYSGESRSKDKNGGNVGDW
                     NREHVWAKSHGDFGTTVGPGTDIHNLRPTDVQVNSTRGNLDFDYGGSSVKGCDGCFKT
                     ANSWEPPDRVKGDVARILFYMATRYEAGDKVDLELNELLNNGSNPYHGKLSVLLEWHE
                     MDPVDAFEMNRNNTIEQLQGNRNPFIDHPEWVKSIWKKAGSNDLRKAS"
     gene            4718..>5164
                     /locus_tag="VFX34_00085"
     CDS             4718..>5164
                     /locus_tag="VFX34_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flp pilus assembly protein CpaB"
                     /protein_id="HEX5563325.1"
                     /translation="MLEAKRRAAIFLLLAFILAAVTGYLVLEKVKELNAELGGMTSIY
                     IAKGSIPSRSPIEASQVTKMDIPNKFVTDSHILSVDEFEHQVSIVPLNPGDIITKNMI
                     KPVSNLQDANNRLVTMYRTDKIQFDQVLTALDRVDIIVSTNDNGNKA"
BASE COUNT         1511 a         1178 c         1320 g         1155 t
ORIGIN      
        1 aaaagatgac attgtccggt acagtagccc acggggagac gtacgtttta tatcataatc
       61 aggcagttga cgaaataaag gataaaggga atcttggaaa ctcaagcgta ataaatttta
      121 acggagatga tccgcttgtt ctcaaaaaaa gcggtactgt aattgactct atcggtcaag
      181 ttggctccac tgctaaatgg ggagttgatg tgacactcgt ccgaaacgct gcagttacga
      241 aaggggattc gaatcccggg gatgcattca accgtgatga agaatggaca gtctatccga
      301 aagacacatt cgatcattta gggttgcatc aaatggatgg aaccccgacg gatccggtag
      361 atcccggaga tccggatgat cctggtgata tttcaacaat cggcgatgtg cgctccaagg
      421 cacttgggga aatcgtaaca atcaaaggaa ccgtagctgc caaattgaag aacacgattt
      481 cagtgcagga cagtacgggc ggaatcgctg tcaggccgac tagcttgaac gtgcaaatcg
      541 gcgatgaagt taccttgaca ggtgaacttg ccgattaccg gggtctgttg caattggaca
      601 aagccaccat cgttgaagtg aagaaagatg ccggcgctcc ggaaccaatc gtcattacgg
      661 gatcggaagt cgatgaagcg gttgaatcac agctcgtcac tgtgcataac gcaattcttt
      721 ccgatgtgca aacgggcagt ggctggtcga attatttagc gacagatgga actggtgact
      781 ttgtcgtccg tgatgaaacg gcgtcattgg gtctcactgt cggaacagcc tatgattcaa
      841 ttactggaat cgtccagcag ttcgacaatg attaccaaat cattccgcgc tcgaccgttg
      901 atgttgtcgt tgacagctcc gtcatccagc cggtcgttgc aagtccagga agcggcacgt
      961 ttgtaggtgg tgtgaaggtt tcattgaaga caggaacgtc tggtgcgaaa attctttaca
     1021 cgctcgacgg gacaagcccg attgaaaacg gggcgacgta tgcgacacca atcgatatcc
     1081 aagaagatac aacagtgaaa gctgtcgtga aaacggatga tgaccgtttt ggagatgtat
     1141 tcactttcac gtatacgata acggaagccc ttcaaatcca cgatatccaa ggggatggcc
     1201 atacatctcc atttgaaaac caaaccgtgg aaggcgtcga agggattgtc acatacaaat
     1261 atgtgctgaa tggctcaacg tactatcata tccagactcc ggacgataaa cgggacggta
     1321 acttgaatac gtcagaggca atcgtccttt acagtggaaa ggatgcttgg aatctttccg
     1381 tcggagatct agtttcggtc accggaaaag tgagcgaata tgcaatcgat gggtatgctg
     1441 accgtcagga aacggatttg aagacgacgc agatcaacgt ccgtgatgac caaggcggta
     1501 aagtgaaaat ccttgagcgg aatgttccgc ttccaacacc tttctttatt aatgaagaaa
     1561 acttgccaca acacgaaatc gcaagtcatc agtttgctga attcaatcct gagaaatatg
     1621 cggctgattt ctgggaaagc cgtgaagcga tgcttgtgca ggtcggcaat gtgaaagcgg
     1681 tcggacctca agagcatggc gatctcgtca cagtgcttga aaatgcggaa acggaaacat
     1741 tgaatggcgg catcctgttg aaggaaaacg acaaaaatgc agagcgtgtc caattccgct
     1801 tggagccgaa cggaccggca cgtgatttcg aagtcgcgac tggagaccgt tttgcaggcc
     1861 cgattacggg agttgtcggc tattcattcc aaaactacaa gatctacacg tctttgagcg
     1921 acatgcagca agcgcattcg aaaggcgaag cagttccaga gaagacgaca attgttaaag
     1981 atgcagataa actgacaatc gcttcttaca acttggaaaa cttctcgaac aatacaaaaa
     2041 cgacttcaaa tgataaagcg agaaaactgg cacgggcatt tgtacaggat atgcaaagtc
     2101 ctgacattat cggtgtaacg gaagtacagg acaataacgg accggatgct ggcgattccc
     2161 gtgcgaatga aagctacgag cgcctcatcc aggcgattgt cgacgcgggc ggtgtccagt
     2221 atgaatacgt caatatcgac ccggtgaaca atgaagatgg cggacagccg aacgcgaata
     2281 tccgtgtcgg tttcctttac aatcctgcgc gcgtttcatt gacggaagga atgccgaaag
     2341 gggatgcgac gactgcggct cattacgaga atggcaagtt gacgcataat cctggacgca
     2401 tcgctccgaa tgacccggcg ttcaacagca gccggaagcc attggccgcg caattcgatt
     2461 tccaaggcga gtcggtcatc gcaatcgtca atcattggaa ttcaaaaaca ggggatacgc
     2521 cattattcgg ctcccagcag ccgccggtct atggcagcga agcgcaacga aaacagattg
     2581 cgcaactcgt ctatgatttc atttccgaaa tcaaagcgga taatcctgag gcaaacatcg
     2641 tttctcttgg agatttcaat gatttccaat ttgcggagcc tctaaaaatc catgaaggca
     2701 gcctcatgac gaacatgatc aaccatgtcg aagcggctga ccgttattcg tatgtacatc
     2761 aaggaaactc ccaagtgctc gaccacattc tagtgtcgaa taacttggcg gatcaaacgg
     2821 aaatcgatat tttgcatatc aacgcagact tcacggacat ggcgggacgt gcaagcgacc
     2881 atgatccggt catggtgcag atcggcttcg aagctcctgt cgtatgggag ccggttccga
     2941 tcaaaaaagt ttacaacctt atcaatgaac ataaaaagca aatcatgatt gctgagccaa
     3001 gcgtctccgt ctatatggat gcgcaatcgt cattgaagga cggcatctac ttgaaagggg
     3061 attatgccga gctgacaggc gaaggattca agtcgaacac cgtcatcctt cagccgaaga
     3121 aaaaaggttt gatcgttgat atgaaaggga cggaaatggg ccatgtgatt gtggaaggct
     3181 cgcatccgct cgaaatcaga ggcgctgaaa acattaagag aatcgacttt gtcaaaggag
     3241 cggacgcttc tgaagtcgtc ttctacaact caaaagggaa gcggatcggc gtgccaacgg
     3301 tgaacaatgc gccggtcatc acgaaaccga tcccgaatca ggaagcgaaa gctggagaga
     3361 cgatttccat cgtcctctcc ggacacttca gcgacccgga cggcgataag ctcacttatt
     3421 ccgcaacgaa agggactgtc gacctcacga agaacgtcct tacgttgcaa ttggaggaag
     3481 gctcccacat cgttggagtg acggcgacgg atggcgataa atccgtaaca gcgagcttct
     3541 cggttactgt cacgaaagcc gaagtgccgg ctgacggcta ttacaaacat gcaatcggca
     3601 aggaggggca ggaattgaaa gctgcccttc acgatatcat ctctgaccac cgaaagctca
     3661 gctatgctca agtatgggat gcactgaagg agacggataa agatccgaac aacccgaaca
     3721 acgtcatcct gttctattca ggtgagtccc gttccaaaga taagaacggc gggaatgtcg
     3781 gcgactggaa ccgtgagcat gtatgggcga aatcgcacgg cgacttcggc acaaccgtcg
     3841 gccccggcac ggatatccac aatttgcgtc cgacagacgt ccaagtgaac agcacacgcg
     3901 gcaacctgga tttcgattat ggcggaagtt cagtgaaggg gtgtgacggc tgcttcaaaa
     3961 ccgccaactc atgggagccg cccgatcgcg tgaaaggcga cgttgcacgg atactgttct
     4021 atatggcaac ccgttacgaa gcgggcgaca aggtggactt ggagttgaat gaactgctca
     4081 acaacggcag caacccatac catggaaaac tgtccgttct attggaatgg catgagatgg
     4141 acccggtcga tgcgttcgaa atgaaccgca acaatacaat cgaacagttg caaggcaacc
     4201 ggaacccgtt catcgaccat ccggaatggg tgaagtcgat ttggaaaaaa gccgggtcga
     4261 acgatctgag aaaagcttcc taatgaaaac aagaccagag gatcaatttt cctttggtct
     4321 ttttctttgc ttgctccatc attcctacga cccttatcct cctatcaaat gatacaagca
     4381 atccagctag cgttaccata gtcttgaagt caaatatctt aaattccagg ctttcaaatt
     4441 atggaaaatg attcataagt tgtgaactat aaaagaaact atgctattcg accttgattt
     4501 ccaccgtagg actataactg aaaagccact ggtaaaactt gttcaaccat cattgccctt
     4561 tggtccaatg actaatgaca tagcacgatc cgagccggtc tgtcacggtt ttgacataca
     4621 atcgtcacaa tgatattaat aatgaacata ggaatctacc aaatggaaga gagggcggaa
     4681 ggttccggca tttctttagg atgaaagagg gatagggatg ttagaagcaa agaggcgcgc
     4741 ggcgatattt ttactattgg ctttcatatt agcagccgtg actggctatc tcgtgctgga
     4801 aaaggtaaaa gagttgaatg cggaactagg tggaatgaca tcgatttaca tcgcgaaagg
     4861 gtcgatccct tcccgttcac cgattgaagc ttctcaagtg acgaaaatgg atatccccaa
     4921 taagttcgtg acagatagcc atattttgtc cgtagatgag ttcgaacatc aagtatccat
     4981 cgttccattg aatccagggg acatcattac aaaaaacatg atcaagccgg tgtcgaatct
     5041 tcaggatgcc aacaatcggc tcgtcaccat gtatcggacg gataagatcc agttcgatca
     5101 agtgctgacg gcattggacc gcgtcgacat cattgtttcg acaaatgaca atggaaataa
     5161 agcg
//
LOCUS       DASULI010000007         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1186743,
            whole genome shotgun sequence.
ACCESSION   DASULI010000007 DASULI010000000
VERSION     DASULI010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186743"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1062
                     /locus_tag="VFX34_00090"
     CDS             100..1062
                     /locus_tag="VFX34_00090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX5563326.1"
                     /translation="MKPTVFITRKLPEEIVAPLKEKFTVRMWDSEEVAVTKAVLREEI
                     AQVDALWTMISDTIDKDMLESAANLKVISNMAVGFNNIDTEAAKEKGIIVTNTPDVLT
                     ETTADLAFGLLLATARRFNSAEKELRQGGWLSWTPMGFTGMDVHGTTIGIVGMGRIGE
                     AVARRAKGFNMQVLYHNRNRKTEAEEEYQAEFAELDDLLKRSDHVVILAPFTEETKGM
                     IGERELSLMKKTATLISVSRGGIVDEEALYDALKNEKIFAAGLDVYETEPVPIRHPLL
                     TLPNVTAVPHIGSASMRTRKAMMALNAQAIFDVLEGNEPKNRVN"
     gene            1256..2998
                     /locus_tag="VFX34_00095"
     CDS             1256..2998
                     /locus_tag="VFX34_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEX5563327.1"
                     /translation="MSAIIVAVAGMIVFALGYRFYSKFVAEKIFRLDPNYVTPAHRYK
                     DGVDFVPTNKLVLWGHHFTSVAGAAPIVGPAIAVYWGWLPAVLWVVLGTVFAAGVHDF
                     GTLVLSVRHKGQSVGTLANRLIGQRAKILFLFIILILVLMVNAVFAWVIANLFIKFPA
                     SVFPVFIQIPLAIWIGTAVYKRQKKLLVPSLIALAVMYIAAVVSSKVGFLQIDLVKYM
                     GGAEGAGLFGLGAVSTAFFIWIVILMVYVYFASTLPVWRLLQPRDFINSHQLVVGLGI
                     LYLGLLFANPTITAPITNATSDVSWFPLLFITIACGAISGFHGLVSSGTSSKQLNKET
                     DARFVGYLGAVGEGILALVSIIAVITFFPNKDAFLATYSSFGAANGSGLTVFVQGASK
                     LATSLHIPADVATTIVSIIVISFAATTLDTSVRLMRYIIAELGMEYNMPKLSKKHVAT
                     SVAVLSSAALVLLPEGPNGFGSGGYLLWPLFGTANQLLAGISLLLIAVWLKRLGRNYL
                     VAVIPMIFLLFMTLLAMFKQVVYEWSWFGSNSNWLLFIFGAIIFVFAVWIILTAFSAL
                     SGKNNQHISTDLEE"
     gene            3015..3272
                     /locus_tag="VFX34_00100"
     CDS             3015..3272
                     /locus_tag="VFX34_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019376111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cory-CC-star protein"
                     /protein_id="HEX5563328.1"
                     /translation="MIDSLKKLISYYEEVISLPHRREIASELRDEEDLFFLLLYSEMI
                     GIPNPAYYYTLELYPHMIEKFHEWHLRMGMEKSPMHGIRCC"
     gene            3287..>4149
                     /locus_tag="VFX34_00105"
     CDS             3287..>4149
                     /locus_tag="VFX34_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX5563329.1"
                     /translation="MDVLTKQIIFVGGKGGVGKSTTAAAIAHRLSASGNRTLLVSTDP
                     AHNVGDIFGRTIGGKPVEVADRLVALEIDPQLETERYITGVKDNIKGVVHSSMMEEVH
                     RQLDTAKASPGADEAALFDKLITIILEEREHFDKIVFDTAPTGHTIRLLSLPDLMGVW
                     IHGLLEKRKKANVNYSRLLNDGEPVEDPIFEVLSERQQRFTRVRELLMDQQTTGFIFV
                     MNPERLPILETEKAIHLLNRHSLQVKSIVVNKMLPAQVEGEFWNERKEHERKYVEMIE
                     RTFKNQDILYVP"
BASE COUNT         1069 a          885 c         1107 g         1088 t
ORIGIN      
        1 atattcaata gatacaattg tgaactcgat agataaccac aaaaatgaac cgactgaatt
       61 gtgctaatat agatgaaaag agaaacggga ggggctggaa tgaaaccgac tgtgtttatc
      121 acaagaaaac tgccggagga aatcgttgcg ccgctaaaag agaagttcac tgtccggatg
      181 tgggactcgg aagaagtggc ggtgacaaaa gctgtactaa gggaagaaat cgcccaagtc
      241 gatgctctat ggacgatgat ttcggatacg atcgacaagg acatgctgga aagcgccgcg
      301 aatttgaaag tgatttcgaa tatggcggtc ggcttcaaca atatcgatac ggaagcggcg
      361 aaggagaaag gcatcatcgt gacgaacacg cctgatgtgt tgacggaaac gacggcggac
      421 ctcgcgttcg gactactact cgccactgca cgccgtttca attcggcgga aaaggaactg
      481 cggcaaggtg gctggctatc gtggacaccg atgggcttta ccggaatgga tgtgcacggg
      541 acgaccattg gcattgttgg tatgggacga atcggcgaag cggttgcgcg tagggcgaaa
      601 gggttcaaca tgcaagtgct ctaccataac cgcaaccgga aaaccgaagc ggaagaagaa
      661 taccaagccg aattcgccga attggacgac ctattaaaac ggtccgacca tgtggtgatc
      721 ctagccccat tcacagaaga gacaaaagga atgatcggtg aacgggagct gtcgctcatg
      781 aagaaaacgg cgacccttat aagtgtgtca cgcggaggca tcgtcgatga agaggcctta
      841 tatgacgcgt tgaaaaatga aaaaatcttt gcggccggac tcgacgttta tgaaacggaa
      901 ccggttccga tccgccatcc attgctcacg ttgccgaatg tcacagccgt tcctcatatt
      961 ggaagcgcaa gcatgcggac gaggaaggcg atgatggcat tgaacgcaca ggcgatcttt
     1021 gatgttttgg agggaaatga accgaagaac cgggtaaatt gaataaagtt ctgcaaggta
     1081 gctcgttcct gccaaccatc cgttacgtat atgcattccc gtcacaatct ttttgtaatg
     1141 gtcataagta gttgtccctg acaatagatt gtactagcta ataataaggg aaataccttc
     1201 gttttgagct actcggaacg aagtgcaaat agaaacggaa tggaggaaag ggagattgag
     1261 tgcaattatt gttgcagtgg cagggatgat tgtttttgcg ttgggttatc gattttattc
     1321 aaaatttgtc gctgaaaaga tcttcagatt agatccgaat tatgtgacgc cagcgcatcg
     1381 gtacaaagat ggggtcgact ttgtgccaac gaataagctt gtcctttggg gacatcattt
     1441 cacatcggtc gcaggcgcag cgccgatagt gggacctgca attgccgtct attggggctg
     1501 gttgccggct gttctatggg tcgttctggg aactgtattc gctgctggcg tccatgattt
     1561 cggaactttg gtgttatccg ttcgccataa aggccaatcg gttggtacgt tagcgaaccg
     1621 cctcattgga caacgggcaa aaattctgtt cttgttcatt atcctcattt tagtgttgat
     1681 ggtgaatgcg gtatttgctt gggtcatcgc taatttgttc attaaattcc cagcaagcgt
     1741 ttttccggtt ttcattcaaa ttcctttggc gatttggatt ggcacagccg tttataaacg
     1801 gcagaaaaag ttacttgttc cttcgctcat cgctttggct gttatgtata ttgcggcagt
     1861 tgtctccagt aaagtgggct tcttgcagat tgatcttgtg aagtacatgg gcggggcgga
     1921 aggagccggt ctgttcggac ttggtgctgt atcaacggcc ttcttcattt ggattgtcat
     1981 tctgatggtg tatgtctatt tcgcttccac actgccggtt tggcgactgt tgcagccgcg
     2041 tgatttcatc aactcacacc aattggtggt cggacttggc attctctact tgggcttact
     2101 tttcgcaaat cctacgatca cggctccaat aacaaatgca acatctgacg tatcgtggtt
     2161 ccctctactg ttcattacaa ttgcatgtgg agcgatatca ggcttccacg gactcgtttc
     2221 gtcaggcact tcatcgaagc agttgaataa agagacggat gcccgatttg tcggctatct
     2281 tggagcagtc ggcgaaggta ttttagcact tgtctccatc attgcggtca tcaccttctt
     2341 cccgaataaa gatgcattcc ttgcaaccta cagcagcttt ggtgcggcaa atggctccgg
     2401 attgactgtt ttcgtacaag gggccagtaa attggcgaca agtttgcata taccagccga
     2461 tgtggcaacg acgatcgtat cgatcattgt cattagcttc gccgcaacaa cattggatac
     2521 ttcggtccgg ttaatgcgat atatcattgc ggaattgggc atggaataca acatgcctaa
     2581 gctgtcaaag aaacatgtgg ctacatcggt tgcggtatta tcaagtgccg cgcttgtgct
     2641 tttaccggaa gggccgaatg gattcgggtc gggcggatat ttactctggc ctttgttcgg
     2701 aaccgcgaat caattgttgg caggcatcag tttactgctg atcgcagttt ggcttaagcg
     2761 gcttggcagg aattatctgg tggccgtcat tccgatgatc ttcctgctat tcatgacact
     2821 gttggcgatg tttaagcaag tggtctatga atggtcatgg ttcgggtcaa actcgaactg
     2881 gttgctcttc atttttggcg caatcatttt cgtctttgcg gtttggatca tattgactgc
     2941 attctccgcc ttgtccggca aaaacaatca gcacatatca acggacttgg aagagtagta
     3001 gggggaggtt acccatgatt gattccctga aaaagctaat ttcgtactac gaagaagtca
     3061 ttagtttgcc ccaccgccgg gaaattgctt cagagttgag ggatgaggag gatctctttt
     3121 tcctcctcct ttattccgaa atgatcggca ttccgaatcc ggcgtattat tacacattgg
     3181 aactctatcc gcatatgatc gaaaaattcc acgaatggca tttgcgcatg ggcatggaga
     3241 aatctccgat gcatggcatc cgctgttgct gaaatgaggg gaaacgatgg acgtattgac
     3301 gaaacaaatc atttttgttg gcggaaaggg cggagtgggc aaatcgacaa cagccgcggc
     3361 cattgcacac cgcttatccg catctgggaa ccgcacactt ttagtatcaa cggatccagc
     3421 tcataatgtc ggtgacatat tcggcagaac gatcggcggc aagccggtgg aggtggcgga
     3481 tcggttagtg gcactggaaa tcgatccaca gcttgagacg gaacggtata ttactggggt
     3541 gaaggacaat attaagggtg tcgtccattc cagtatgatg gaggaagtgc atcgccagtt
     3601 ggacacggca aaagcttcgc caggagcgga cgaggcggcc cttttcgata aactgatcac
     3661 gattatcttg gaggaacgcg aacattttga caaaattgtg ttcgacactg cgccaactgg
     3721 ccatacaatc cgcttgcttt cgcttcctga cctgatgggt gtgtggattc atggcttgtt
     3781 ggaaaaacga aaaaaggcga atgttaatta ctcgcgtctg cttaatgacg gtgagccggt
     3841 ggaagatccg atttttgaag tgttatcaga gaggcagcaa cgctttaccc gcgtccgtga
     3901 gctattaatg gaccagcaaa cgacaggttt tattttcgtt atgaacccgg aacggctgcc
     3961 aattttggaa acggaaaaag cgatacattt gctgaaccgg cattcacttc aagtgaagtc
     4021 gattgtcgtc aataagatgc tccctgcaca ggtcgagggt gagttttgga atgaacggaa
     4081 agaacatgag cggaaatatg tagagatgat agagaggacg ttcaagaacc aagacatcct
     4141 ttatgtccc
//
LOCUS       DASULI010000008         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_992098, whole
            genome shotgun sequence.
ACCESSION   DASULI010000008 DASULI010000000
VERSION     DASULI010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992098"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFX34_00110"
     CDS             complement(<1..765)
                     /locus_tag="VFX34_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5563330.1"
                     /translation="MDPMLDRVPPHNNEAEQSVIGAIFLEPQALITAGEILIPEDFYR
                     MAHQKIFSTMLRLSDKGQAIDVVTVTEELSARKELEDVGGISYLTELANAVPTAANVV
                     HYAKIVEEKALLRRLIRVATSIVEDSYMREDEVEALLSESEKRMMEVANRKNAGDFKH
                     IKDVLVQTYDNIELLHTRKGDITGVPTGFRDLDRITAGFQKNDLIIVAARPSVGKTAF
                     ALNVAQNVAVKAGENVAIFSLEMGAEQLVMRMLCAEG"
     gene            complement(778..1227)
                     /gene="rplI"
                     /locus_tag="VFX34_00115"
     CDS             complement(778..1227)
                     /gene="rplI"
                     /locus_tag="VFX34_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5563331.1"
                     /translation="MKVIFLQDVKGKAKKGEVKEVSVGYAQNFLFKNNLAVEATPANI
                     SKLEGQKKRQAKEAAEELEEAKQLKEKLEQLTVELKAKAGEGGRLFGSITTKQIADAL
                     QKKEGIKIDKRKMELPDAIRALGYTNVPVKLHQEVTASLKVHVTEES"
     gene            complement(1224..>2064)
                     /locus_tag="VFX34_00120"
     CDS             complement(1224..>2064)
                     /locus_tag="VFX34_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX5563332.1"
                     /translation="LDKSVIRLMDEIRKDEELFKRFISPDDALGLMTEHSLMIVVDTH
                     KPSFVIDERVVDRAEKVVVIDHHRRGEEFIKNTMLVYMEPYASSTAELVTELLVYQPK
                     NEKLTMLESTALLAGIIVDTKSFTLRTGARTFEAASYLRTNGADTILVQQMLKEDIDT
                     YITRAKIIENVQLYDHGIAIAKGLDDVVYSPVLVAQTADTLLSMQGVAASFVIALRAD
                     GKVGISARSLGDLNVQVIMEELGGGGHLTNAACQLSVDTIEEAEHMLKAALEIETERG
                     NEE"
BASE COUNT          475 a          583 c          407 g          599 t
ORIGIN      
        1 tccttccgca cagagcatac gcatgacaag ctgctccgcc cccatctcca aactgaagat
       61 ggcgacattc tcgcctgcct taaccgccac attctgcgcc acgttcaacg cgaacgccgt
      121 cttaccaacg gacggacgtg ctgctacaat aatcaaatcg ttcttctgga aaccagccgt
      181 aatccggtcc aagtcccgga atccggtcgg tacacctgta atatcgcctt ttcgcgtatg
      241 tagcaactca atgttgtcat acgtctggac aagtacgtcc ttaatatgtt taaagtcacc
      301 ggcattcttg cggttggcga cctccatcat tctcttttca gattcagata ggagggcctc
      361 gacttcatcc tcacgcatat aactatcttc gacaatcgat gttgctacgc gaatcaatcg
      421 ccgtaagaga gccttctctt ctacgatttt cgcatagtgg acaacgtttg ccgctgtcgg
      481 aactgcattc gccagctccg ttaaatatga aatgccacca acatcctcta attccttacg
      541 tgcagaaagc tcctcggtca ccgtcacgac atctatcgct tgtcccttgt cgctcagccg
      601 cagcatcgtt gaaaagatct tttggtgtgc catccggtaa aaatcttccg gaattaaaat
      661 ctcacctgct gtaatgagcg cttgtggttc gaggaagatc gctccgatca ccgattgttc
      721 cgcttcgtta ttatgcggtg gaacacgatc gagcattgga tccatctttc tctcccctta
      781 cgattcttct gtcacatgga cttttagtga tgccgtaact tcctgatgaa gttttacagg
      841 aacgtttgta tagccaagcg cacggattgc atccggcagc tccattttcc gtttgtcgat
      901 tttgatgcct tcttttttct gcaaagcatc cgcaatttgt tttgtcgtga tcgacccgaa
      961 caatctgccg ccttctccgg ctttcgcctt gagttcaacc gtcaattgct cgagcttctc
     1021 tttcagttgc tttgcttctt ccaactcttc agcagcttct ttcgcttggc gcttcttctg
     1081 gccttccagc ttgctaatgt ttgctggtgt cgcctcgact gcaaggttgt ttttgaaaag
     1141 gaagttctgt gcatagccaa cagaaacttc tttcacttca ccttttttcg cttttccttt
     1201 tacgtcttgc aaaaagatta ctttcattct tcatttcccc tttctgtctc aatttctagt
     1261 gcagctttga gcatgtgctc tgcttcttct attgtatcaa cagatagctg gcatgcggca
     1321 ttggttaaat gtccacctcc gccaagctct tccattataa cctggacatt taagtccccg
     1381 agtgacctcg cgctgattcc aactttccca tccgctcgga gcgcaatgac aaacgaagcc
     1441 gcaaccccct gcatggagag aagcgtatct gccgtttgcg caacaagaac aggactatac
     1501 acaacgtcgt ccaatccttt ggcaatggca atgccatggt catacaattg aacgttctca
     1561 ataattttag cacgagtaat atacgtatcg atatcctctt tcaacatttg ctggacaaga
     1621 atcgtatccg ctccgtttgt tcgcaaatac gacgcagcct caaacgtacg cgctcctgtc
     1681 cgaagcgtaa agctcttcgt gtcgacaata ataccggcaa gaagcgctgt tgattccaac
     1741 atcgtcagct tttcattctt cggctggtac acaagcaact cggtcacaag ctcggctgta
     1801 gaagacgcgt aaggctccat atagacgagc atcgtattct taatgaattc ctcgccgcga
     1861 cgatgatggt cgatgacaac aaccttctca gcccggtcca caacccgctc atcaatcacg
     1921 aagcttggtt tgtgtgtatc cacgacaatc attaatgaat gctcagtcat gagccccaac
     1981 gcatcatccg ggctaataaa ccgcttgaac agctcctcat ccttcctgat ttcatccatc
     2041 aggcggatga cacttttatc aagc
//
LOCUS       DASULI010000009         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1770189,
            whole genome shotgun sequence.
ACCESSION   DASULI010000009 DASULI010000000
VERSION     DASULI010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770189"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..897)
                     /locus_tag="VFX34_00125"
     CDS             complement(94..897)
                     /locus_tag="VFX34_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5563333.1"
                     /translation="MVKIYIDPGHGGTDPGAIGNGLREKDVTLAIATRVKNMLVNEYS
                     NATVRMSRTGDQTVDLPQRTNDANAWGADYYLSIHINAGGGTGYEDYIHSALPDSSTT
                     AQLRNTIHAEIVKLIDMPDRGKKKENFHVLRESWMPAMLTENGFIDNATDAAKMKTAA
                     WLDKAARGHVNGLAKALGLQKVSTPPPTGDVLYRVVTGSFTTRANADQQVAALKAKGF
                     DSFIDIVVLNGTTYYRVITGSFADKANADKQVADLKAAGFDAFIIVVNL"
     gene            complement(1002..1175)
                     /locus_tag="VFX34_00130"
     CDS             complement(1002..1175)
                     /locus_tag="VFX34_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563334.1"
                     /translation="MSQKSRDEQNGAKLEQNSLELEHNSMQLRHKSPRLRQKPIELEQ
                     NELAPTGKEVMPE"
     gene            complement(1203..1403)
                     /locus_tag="VFX34_00135"
     CDS             complement(1203..1403)
                     /locus_tag="VFX34_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BhlA/UviB family holin-like peptide"
                     /protein_id="HEX5563335.1"
                     /translation="MATKELLGYFLTQGPFAVLFVWLFWYQVKSSESREKRYQFLLEA
                     MTDKYDDIVEEIREVKERLPKE"
     gene            complement(1544..1966)
                     /locus_tag="VFX34_00140"
     CDS             complement(1544..1966)
                     /locus_tag="VFX34_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004438552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX5563336.1"
                     /translation="MTKEEMEQLVKDYHWMINSVKVIREGMNEVGEGMTARYGLEAAM
                     PKASGGRPSDPIYREFTRRERRLKKVYEYERKIQLVQERIPLITEDREIEVLHWLLEG
                     KSLRWIGRHMQISDRQIGRIKDAVMDKMSQMSHVPQMS"
     gene            2106..2441
                     /locus_tag="VFX34_00145"
     CDS             2106..2441
                     /locus_tag="VFX34_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017470665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5563337.1"
                     /translation="MYSIGREIKKIRSEHKLSQEEFADRVNQKLGLSITKGMVSKWES
                     DSIEPKTRMIKAIAETFHVSLDQMLGMNVPEKPSAVETIAAHIDDDLTEEELADIKKY
                     IQFIKSQRK"
     gene            2451..2900
                     /locus_tag="VFX34_00150"
     CDS             2451..2900
                     /locus_tag="VFX34_00150"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX5563338.1"
                     /translation="MTYESLIAQYPHLVIKEVKTLPSGLAGLYMDNVVLIDKNRSHYE
                     KHCILAEEIGHYETTYGDITDLQKLTNLKLELAARRWGYEKIVSLDKLIECYESGFKT
                     VEEVCTHLEVTEDYLMTSIAHYHSRFGVSTIHNGYEIFFDPLYLRKQ"
BASE COUNT          746 a          782 c          681 g          907 t
ORIGIN      
        1 ggagtgaatg gtgatttgac gaatgaatac tttgatggtt gaatggaata tgtaaagcgc
       61 ctgtacgtat ccgactgcac aggcgcttgt ctattataga ttaaccacaa tgataaacgc
      121 gtcaaatccg gcggctttca agtcggcgac ttgcttgtcc gcgttcgcct tatccgcaaa
      181 tgaaccggtg atgacccggt aatacgtagt gccattcagg acaacgatgt caatgaagct
      241 gtcgaagcct ttcgctttga gtgcggcgac ttgctgatcg gcgttggctc ttgtcgtgaa
      301 ggagccagtc acaacacgat acaacacatc acccgttggc ggcggagtgc ttactttttg
      361 aagcccaagt gctttggcaa gtccattgac atggcctctt gccgccttgt caagccatgc
      421 cgccgttttc atcttcgctg catcggtcgc attgtcgatg aagccattct ccgtcagcat
      481 cgcaggcatc cagctttccc gcagtacatg gaagttctcc ttcttcttcc cgcgatccgg
      541 catgtcgatc aacttcacga tttccgcatg aatcgtattc cgcaactggg cggtcgtcga
      601 agaatccgga agcgcgctat ggatataatc ctcatacccc gttcccccgc cagcattaat
      661 atggatgctc aaataataat cggcgcccca tgcattcgcg tcattcgtcc gctgcggcaa
      721 atcaacagtc tgatcgcccg tccggctcat ccgcaccgtc gcattggaat attcgttcac
      781 aagcatattt ttgacgcgcg ttgcgattgc gagcgtcaca tctttctccc ttaaaccatt
      841 tccgatagcc cctggatctg ttcctccatg ccctggatcg atatagattt taaccacttt
      901 tcatcttcct ctcgtttttt tgttttttat tctgtgacaa tctgacatcc cggcagtttt
      961 tgagtgtcta catgggaatc gcctcctttg aatgacgttt tttattcagg catcacctcc
     1021 tttccggttg gcgcaagttc gttttgctcg agttcgatcg gcttttggcg caatctcggc
     1081 gacttatggc gcaattgcat ggagttatgc tcgagttcga gtgagttttg ctcgagtttc
     1141 gccccatttt gctcgtctcg ggacttctga ctcatccatt tcaccaataa aaaaagcgat
     1201 gcctattctt tcggcaaccg ctctttcact tctcgtattt cttcaacaat atcgtcgtat
     1261 ttgtccgtca tcgcttccaa taaaaactga taacgtttct ctcttgattc actgcttttc
     1321 acttgatacc aaaacagcca cacgaacagg acggcaaacg gaccctgtgt caaaaaataa
     1381 cctagcagct ctttcgtagc catgccaatc ctccttttat ttcaggaaaa caaaaagaac
     1441 gccccaatgg acgctctttt gaatttcctg ttagttgtgt tgcacccggc cgatttcctt
     1501 ggcctgcacc cattgtaacc cggaagaatt tcattcgtca tttctacgac atttgcggca
     1561 catgcgacat ttgcgacatc ttgtccatga cggcgtcttt tattcggccg atttggcggt
     1621 ccgatatttg catatgcctg ccgatccagc gcaagctctt cccctcaagc agccaatgca
     1681 gcacttcaat ttcacggtcc tctgtgatga gtggaatccg ttcttggact agctggattt
     1741 tgcgctcata ctcgtacact ttcttcaatc gcctttctct tctggtgaat tcccggtaaa
     1801 tcggatcgct aggtcggccg ccgctcgctt tcggcatcgc cgcttccaat ccgtagcgcg
     1861 ccgtcattcc ctcaccgact tcgttcattc cctcgcgaat gaccttaacg ctgttgatca
     1921 tccaatgata atccttcact aattgctcca tttcctcctt cgtcataaca atcacccctt
     1981 gttttagttt cattaatgaa acctagtagc catactttat cacgcgaatg tttcatttac
     2041 aactacttag ttgtcacaag agaaactttg gtatataata accataacga acagggggtt
     2101 tcaagatgta ttctataggc cgcgaaatta aaaagatccg gagcgaacat aaattgtccc
     2161 aagaggaatt tgctgatcgg gtcaatcaaa agctaggtct ctcgattacg aaagggatgg
     2221 tttcgaagtg ggagtccgat tcaattgagc cgaaaacgag gatgatcaaa gcgattgcgg
     2281 agacattcca tgtttcattg gatcagatgc tcggaatgaa cgtgccggag aaaccatcgg
     2341 cggttgaaac gattgccgcc catatcgatg atgatctgac ggaggaagaa ctggcggaca
     2401 tcaaaaaata catccaattc atcaagtcgc aacgaaagtg aggtggttcc atgacctatg
     2461 aatccctgat tgcccaatac ccccacctgg tcatcaaaga agtcaaaacg cttccatccg
     2521 ggcttgcggg cctttacatg gacaatgtcg tactcattga taagaatcgg agccattatg
     2581 aaaagcattg cattttggcg gaagaaatcg gccattatga aacgacatat ggcgatatta
     2641 ccgatcttca gaaactgacg aatttgaagt tggaactggc cgcgagaaga tggggatatg
     2701 aaaagatcgt ctcgttggat aaattgatcg aatgctatga gtcgggtttc aagacggttg
     2761 aggaagtgtg tacgcatctt gaggtgacgg aggattattt gatgacatcc attgcccatt
     2821 accactcccg tttcggcgtt tcgacaatcc ataatgggta tgagattttc ttcgacccgc
     2881 tttacttgcg aaagcagtaa ttttttttag gtgtaaatcg aacatacgtt tcatttgacg
     2941 actgaaaaga gaccacatct atattagatt tggtctctct ttgaagttta tgatcactta
     3001 ttcggattaa tgagcgcttg ccgccgttta tgagcgctat ttcggattta tgagcggtcc
     3061 acggccattt atgagcgctc attccaattt ttgagcgctc ggctacggtt tttgag
//
LOCUS       DASULI010000010         9165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_446812, whole
            genome shotgun sequence.
ACCESSION   DASULI010000010 DASULI010000000
VERSION     DASULI010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446812"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFX34_00155"
     CDS             complement(<1..977)
                     /locus_tag="VFX34_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563339.1"
                     /translation="MTFRQFAFRNVMRNRRIYAAFFMASVFSVMVFFLYSMLLFHPSI
                     EGSAVQDIAVLGMGIAEIILYIFTMFFLFYSMRAFLQARTKEFGILLHLGMEKRQLHR
                     LIFIETLIIGAASIGIGTFLGYMFSKFFFMVVRRIVMLPALPLYLSWEPFALTVGAFL
                     SLFIIISWVAPVFIRTGKLDELIRGDGVGMETHGFSKIRGVIGLILLGLSYGMAAFTS
                     NSIVIGLVFLLPPLATLGTYFFFTDSLPFILHLFKVRREFYWRHFRLLAISEGVIRLK
                     ENARMFFIVTIVSTVAFMSVGILASLTSFASQYREMNPLGLVYESFPGN"
     gene            complement(1008..1778)
                     /locus_tag="VFX34_00160"
     CDS             complement(1008..1778)
                     /locus_tag="VFX34_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563340.1"
                     /translation="MKKAIVELNQVTKIYEGKVMHRALNRLDFEAEEGEFIAIMGPSG
                     SGKTTLLNIISTIDMPTYGRVTIDGVEPEALNSNELALFRRHNFGFVFQDINLLKSLT
                     VEENIVLPLTLDGLPITEMQKRVDKLAGQLSLKDILNRRPDELSGGQAQRTAVGRALI
                     HKPKLILADEPTGNLDSKSAKEVLELLSLVNQTERTTIIMVTHDPIAASYCDRVLFIK
                     DGEFFNEIYKDERRQTFFQRILNVLSLLGGNVHDLRML"
     gene            complement(1732..2781)
                     /locus_tag="VFX34_00165"
     CDS             complement(1732..2781)
                     /locus_tag="VFX34_00165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5563341.1"
                     /translation="MRQIILFLREHLSWIIFEMLLVTFILVLYWLDGFRGLNTAIYSI
                     IMSLLLTGGFLVGKFITRRSYYAAILRKPEKMEDALIRNVQSTEQRQSTKFMRGLYGL
                     YQAEVQSLYASQHRQLQFVNQWVHQMKTPISVINLLLQEDGELDKRSIREEVDKIQRG
                     LDVVLVNARLETFEEDMQIEQVGLKSLVQQIVTEHKRLFITNGVFPVISIDESLVVAT
                     DPKWMKIAIGQFITNAVKYTFEKGKCLYLSARQTNIGFELTIRDEGVGIPASDLKRVT
                     KAFFTGENGRLTGESTGMGLYIADEVCLKLGHPLTIDSEVGKGTSVKILFANGEIGDP
                     YEESDRGTESSDEDL"
     gene            complement(2778..3482)
                     /locus_tag="VFX34_00170"
     CDS             complement(2778..3482)
                     /locus_tag="VFX34_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5563342.1"
                     /translation="MRNHRIFIVEDDKKIARLLADTLRKYQYEVAIVEDFDHVEAECM
                     AFDPHLILLDINLPSYDGYYWCRQLRQTTTCPIIFISARSGDMDQVFALENGGDDFIT
                     KPFHYEIVLAKIRSHLRRAFGEYAPSQGERTVKTGSLELFIERMELHLRNEAIPLQKK
                     ECVILELLMEASPKVVSRETLLEELWDDQSFVDENTLNVNMTRVRKKLADYGVQSVIE
                     TVRGSGYRFLVAPEES"
     gene            complement(3610..3783)
                     /locus_tag="VFX34_00175"
     CDS             complement(3610..3783)
                     /locus_tag="VFX34_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563343.1"
                     /translation="MINWRFDGKVSRIGGNTRFGGKVIRIGGNATRFGGKVVRIGGNQ
                     PRVGDKVIKVGDN"
     gene            complement(4013..5005)
                     /locus_tag="VFX34_00180"
     CDS             complement(4013..5005)
                     /locus_tag="VFX34_00180"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEX5563344.1"
                     /translation="MYFVDNKGITDPRINLAIEEYVLKNMDIDKDSFLLFYINEPSII
                     IGKNQNTVEEINTEYVDANGIHVVRRLSGGGAVYHDLGNLNYSFITKDDGESFRNFKK
                     FTEPVIKALADMGVKAELLGRNDILVEGRKVSGNAQFATQGRMFTHGTLMFDTEIERV
                     VSALKVRKDKIESKGIKSIRSRVANISEFLEEPMTIEQFRLEILKSIFEGEENVQYWE
                     LTDEDWDNIRALSAERYGNWDWNYGKSPKFNMQHSHRFPVGGIDVRLQVEKGKIDDVN
                     IYGDFFGVGDVSVVEDSLKGVQYDRESITKALDAVDIPTLLGGITKEEFINLIY"
     gene            5140..5265
                     /gene="yhfH"
                     /locus_tag="VFX34_00185"
     CDS             5140..5265
                     /gene="yhfH"
                     /locus_tag="VFX34_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhfH"
                     /protein_id="HEX5563345.1"
                     /translation="MIESIVEFFRNLPAKACEVCGENIEEQHECYSNKCDECNIV"
     gene            5345..5470
                     /gene="yhfH"
                     /locus_tag="VFX34_00190"
     CDS             5345..5470
                     /gene="yhfH"
                     /locus_tag="VFX34_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhfH"
                     /protein_id="HEX5563346.1"
                     /translation="MLENVIEFFKNLPAKTCVECGKEIEEQHECYCNKCDECNII"
     gene            complement(5571..6437)
                     /locus_tag="VFX34_00195"
     CDS             complement(5571..6437)
                     /locus_tag="VFX34_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5563347.1"
                     /translation="MKKILCIMSMAILLSACNIGNDWDNPMPEKGFLIIGHRGASAYE
                     PENTIPAFELAEDLNADYVELDVHLTKDGELIVMHDDDVKRTTEAAGKIKDFTLAELK
                     QLTADEKKGKKIAASGRNEEAYEIPTLREVVEQLKGDLRFVIELKDTEQYPGIEEELV
                     RLMKEQELIGIDAEGYPEAVILSFDKKALKKVQKIEPEIPLIQLVSFDEGEEAALTKK
                     ELKDILSYASGINVSYEALTPPFVNTMHDKGLAVYAYTVNDAEAALRLKAMGVNGIAT
                     DEPDLLDEDKNR"
     gene            complement(6488..7909)
                     /gene="hemY"
                     /locus_tag="VFX34_00200"
     CDS             complement(6488..7909)
                     /gene="hemY"
                     /locus_tag="VFX34_00200"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX5563348.1"
                     /translation="MSEKKKRVVIVGGGITGLSAAFYMQKAAREQNLPIEVTLVEATN
                     RLGGKIQTVRRDGFVIERGPDSFLIRKKSMDTFAKDVGIGEDLVRNATGQAYILMNGR
                     LQPIPGGSVMGIPTKVGPFLKSDLFSLAGKLRAAGDFVLPRSGIEGDQSLGHFFRRRF
                     GAEIVENLIEPLLSGVYAGDIDHMSLQSTFPQFYEVEKKHRSLILGMKKTTPKQVPQK
                     DGHGASPKGAFHSLRNGLESIVEAAEQQLEPGTVRKGIRIESISKQGEQTVLELNTGE
                     KIVADSVILTTGHMAASQLFAPHGLLQGLNEIHTTSVATIALAFPEENIVQDTEGTGF
                     LVSRSSDYSITACTWVHRKWPTSTPEGKVLLRAFVGRVGEDAIVDLPDDEIERIVLED
                     LSKIITIKGKPDFTVVTRFKEDRPQYRVGHRQRIDAARAELKREFPNVKLAGASYDGV
                     GLPDCVDQGKAAVEEVIADLFAK"
     gene            complement(7931..8863)
                     /gene="hemH"
                     /locus_tag="VFX34_00205"
     CDS             complement(7931..8863)
                     /gene="hemH"
                     /locus_tag="VFX34_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5563349.1"
                     /translation="MTKRKMGLLVMAYGTPYKEEDILPYYTHIRRGRPPAPEQLEDLK
                     ARYQAIGGISPLAKITENQANALGDRLNAMQDEIEFNVYVGLKHITPFVEEAVEQMEA
                     DGITEAVSIVLAPHYSSFSVKSYNERAKEEAEKHGVSMTSVESWYKQPKFIEYWSEKI
                     RGTYDSMSDAEREKACLVVSAHSLPEKIKQFGDPYPEQLEETAKLIAEAAGVEEYAVG
                     WQSEGQTGEPWLGPDVQDLTRDLHEQKGYETFVYTPVGFVSDHLEVLYDNDHECKVVC
                     DEIGASYYRPAMPNTDPLFINAMADAVLEQLKEA"
     gene            complement(8897..>9165)
                     /locus_tag="VFX34_00210"
     CDS             complement(8897..>9165)
                     /locus_tag="VFX34_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX5563350.1"
                     /translation="VVGLDWRLSVKEAGERGLTKTLQGNLDPALLLADWDIIEERAKV
                     IVEQGVEHGSHIFNLGHGVFPEVQPATLKRLTEFVHEYSAQLRK"
BASE COUNT         2100 a         2523 c         2165 g         2377 t
ORIGIN      
        1 tcattgcccg gaaagctttc gtacacaagg ccgagcgggt tcatttcccg gtattgggag
       61 gcgaatgaag tgagcgatgc gaggattccg acggacatga acgcgaccgt cgagacgatt
      121 gtcacgatga aaaacatccg ggcgttctcc ttcagcctga tgacgccttc ggaaatcgcg
      181 agcaggcgga aatgccgcca atagaactcc ctgcgcactt tgaacagatg gaggatgaac
      241 ggcaaggaat cggtgaaaaa gaaatacgtt ccgagcgtcg cgagcggcgg cagcaggaag
      301 acgagtccga tgacgatgct gttcgacgtg aaggcggcca tcccatagga aagtccgagt
      361 aggatgagac cgatcacgcc gcggattttt gagaaaccat gcgtctccat gccgacgccg
      421 tctccgcgga tcaattcatc aagcttgcct gtccggatga aaaccggtgc gacccatgaa
      481 atgatgatga acaggctcag gaaagcgccg accgtcaatg cgaacggctc ccaggataaa
      541 tatagcggga gcgctggcag catgacgatc ctcctcacga ccatgaagaa gaacttcgaa
      601 aacatatacc ctaaaaaggt gccgatcccg atcgatgccg cgccgatgat gagcgtttcg
      661 atgaaaatga gccggtgcag ctgccgtttt tccatcccta aatggagaag gatgccgaac
      721 tccttcgtcc tcgcctgcag gaaggcccgc atcgaataga acaggaagaa catcgtgaat
      781 atgtaaagga tgatttcggc gatgcccatg ccgagcaccg caatgtcctg caccgcactg
      841 ccttcgatag acggatggaa gagcagcatg gaataaagga agaacaccat gaccgaaaaa
      901 acgctagcca tgaaaaaggc ggcgtagatt cgacgattgc gcatgacgtt gcggaaagcg
      961 aactggcgaa acgtcatcgc gcactcctcc ctatccggtt gtccgtttta cagcatccgt
     1021 agatcatgga cattccctcc aagtagactg agcacattca gaatgcgctg gaaaaacgtc
     1081 tggcgccgct cgtccttgta gatttcattg aaaaattcgc cgtccttgat gaacagcaca
     1141 cggtcgcaat agcttgccgc aatcggatca tgcgtcacca tgatgatggt cgtccgctcg
     1201 gtttgattga caagactgag caattcaagg acttcctttg ccgatttcga atcgagattc
     1261 cctgtcggtt catccgccag aataagcttc ggcttatgga tcagtgcgcg tccaacagcg
     1321 gtccgctgcg cctgtccccc cgacagctcg tccggccgac ggttgaggat atccttcaaa
     1381 ctcaattgcc ctgccaactt atcgacccgt ttttgcatct ctgtgatcgg cagcccgtcc
     1441 aacgtgagcg gcaagacgat attctcttcc accgtcaagc ttttcagcag attgatgtcc
     1501 tggaagacga agccgaaatt atgccgcctg aacagcgcca attcattcga attcaacgct
     1561 tcaggctcga cgccgtcaat cgtgacgcgt ccgtatgtcg gcatatcgat tgtcgaaatg
     1621 atgttcaaca atgttgtctt tccgctccct gaaggcccca tgatggcgat gaattccccc
     1681 tcttcagcct caaagtcgag tcggttcagt gcgcggtgca tcactttccc ttcatagatc
     1741 ttcgtcactt gattcagttc cacgatcgct ttcttcatac ggatcaccta tctctccatt
     1801 tgcaaacaaa atcttgacgg aagtcccttt ccccacttcc gaatcaatcg tcaatggatg
     1861 ccctagtttc aaacagacct catcggctat atacagcccc attcctgtcg attcacccgt
     1921 cagccggcca ttttcacctg taaagaatgc cttcgtcacc cgcttcagat cagatgccgg
     1981 aatgccgacc ccttcatcgc gaatcgtaag ctcgaagcct atattggttt gtctcgctga
     2041 caagtaaagg cacttccctt tttcaaacgt atatttcaca gcatttgtaa tgaactgccc
     2101 gatagcgatc ttcatccatt ttgggtccgt cgcgacgacg agggactcgt cgattgatat
     2161 gaccggaaac acgccgttcg tgatgaacag ccgcttatgc tccgttacga tctgctggac
     2221 gaggctcttc aaaccgacct gttcgatctg catgtcttct tcgaatgttt caagccgtgc
     2281 gttgacaagc acgacatcga gcccgcgctg gattttatcg acttcctcgc ggatgctccg
     2341 cttgtcgagt tcgccatctt cctgaagcag caagttgatg acggaaatgg gcgtcttcat
     2401 ctgatgtacc cattgattga cgaattggag ctgccggtgc tgtgaagcgt atagcgactg
     2461 cacttccgct tggtacaagc cgtacaaacc ccgcatgaat ttcgtcgatt gacgctgctc
     2521 tgtcgactgc acgtttcgga tgagcgcgtc ttccatcttt tccggcttgc gtaggatcgc
     2581 ggcataatac gatcgccggg tgatgaattt cccgacgagg aagccccccg tcagcagcaa
     2641 gctcatgatg atcgaataga ttgccgtatt gagcccgcgg aagccgtcca gccaataaag
     2701 gacgagaatg aatgtgacaa ggagcatttc gaagatgatc caggataaat gctcccttag
     2761 gaataaaata atctgcctca tgattcctcc ggcgcgacga ggaaccggta gcccgacccg
     2821 cggactgtct cgatgaccga ttgcacgcca taatccgcaa gctttttccg cacgcgcgtc
     2881 atgttcacgt tcagcgtgtt ttcgtcgaca aacgattgat catcccacaa ttcctcaagc
     2941 agcgtttccc gcgaaacgac tttcggtgat gcctccatca acagctcaag gatgacgcat
     3001 tccttcttct ggagcgggat ggcttcgttg cgcaaatgga gttccatccg ctcgatgaac
     3061 agctcgagcg aaccggtctt caccgtacgt tccccctggc tcggcgcgta ctccccgaat
     3121 gcccttctca aatggcttct gatcttcgca aggacgattt cgtaatggaa aggtttcgta
     3181 atgaaatcgt cgcccccgtt ctcgagcgca aatacttggt ccatatcgcc ggaacgagcg
     3241 gagatgaaaa tgatcgggca cgtcgtcgtc tgccgcaact gacggcacca gtagtagccg
     3301 tcgtatgaag gcaaattgat atcgaggagg atcaaatgcg gatcgaacgc catacattcc
     3361 gcctccacat gatcgaaatc ctccacgatc gccacttcat attgatattt gcgcaacgta
     3421 tccgccaaca gccgcgcaat ttttttatcg tcctcaacaa tgaagatacg atggttccgc
     3481 atcgcaaatt cccccttcgc tattccatac attatatcgc aaaaatggaa tgcacgctcg
     3541 gtgggcggag ggggagtggt ttggaaatgg gttatcaccg actttgggag gtttatcacc
     3601 gactcttggc tagttatcac cgactttgat gactttatca ccgactctcg gctggttacc
     3661 accgattcgg acgactttac caccgaatct cgtcgcatta ccaccgattc ggatgacttt
     3721 accaccgaat ctcgtgttac caccgatacg ggagacttta ccatcgaatc tccaatttat
     3781 caccgctcag ggagagttta tcaccgatta aaacgacttt atcaccgctc gggagaggtt
     3841 tatcaccgct tagactgact ttatcaccgc tcaagcattg tttatcacca ctcagccatt
     3901 ttccggataa aacctcccca ttccaaaatg ctacacatga taactacact acttaatgca
     3961 agccagcata taataaggca aaataaaaaa ccggctcgca ccggttttca acttaataga
     4021 ttaaattaat gaattcctct ttcgtgatcc cgcccaatag cgtcgggata tcgactgcgt
     4081 cgagcgcttt ggtgatggac tcgcgatcgt attggacgcc cttcaggctg tcttcgacga
     4141 ccgatacgtc gccgacgccg aaaaagtcgc cgtagatgtt gacgtcatcg atcttccctt
     4201 tctcgacttg caggcgcacg tcgatgccgc cgacagggaa gcgatgggag tgctgcatat
     4261 tgaacttcgg cgatttgccg tagttccagt cccaattgcc gtatcgctct gctgataaag
     4321 cgcggatgtt gtcccaatct tcatcggtca gttcccaata ttggacgttc tcttcgcctt
     4381 cgaagatcga cttcagaatt tccaaccgga attgttcaat cgtcattggc tcttctagaa
     4441 attctgaaat gttcgcgaca cggctgcgga tcgatttgat ccctttcgat tcgatcttgt
     4501 ccttccgtac cttcaacgca gacacgacac gctcaatctc cgtatcgaac atcaatgtcc
     4561 cgtgtgtgaa catgcggcct tgcgttgcga attgcgcatt gcctgaaacc ttacgcccct
     4621 cgacgagaat gtcattgcgg ccgagcaatt ccgctttgac gcccatatcc gccaatgctt
     4681 tgatgaccgg ctcggtgaac ttcttgaaat tgcggaatga ctcgccgtca tctttcgtga
     4741 tgaaactgta gttgaggttc cctaaatcat ggtacacagc accgccgccg gaaagtctgc
     4801 ggacaacgtg gatgccgttc gcatcgacat actctgtatt aatctcctcg accgtattct
     4861 ggttcttccc gatgatgatc gacggttcgt tgatatagaa cagtaaaaag gaatctttgt
     4921 cgatgtccat gtttttcaat acatactcct cgatcgcaag gttgattctt ggatctgtaa
     4981 tgcctttatt gtcaacgaaa tacacaataa tcgctcccta tgtaaatgaa ttcaattcta
     5041 ttttatccga aaagaacaga aaagtgttga catttgatta gctgttatat tacaatcaca
     5101 ctatcaaatt gagttataga acacgaggag gatgatgaaa tgatcgagag tatcgtagag
     5161 tttttcagaa acctgcccgc caaagcctgt gaagtttgtg gagagaatat cgaagagcag
     5221 catgaatgct atagcaataa atgtgacgag tgcaatatcg tctaattttt ttagctatgc
     5281 tgttataata cactagccca attacctatt tagtattagt acaaaaagac aggagggaat
     5341 accgatgctg gagaatgtca ttgagttttt caagaacctg cctgctaaaa cgtgtgtgga
     5401 atgcggaaaa gagatcgagg agcagcatga gtgctactgc aataaatgcg atgagtgcaa
     5461 catcatctaa tacagaagag gactgcccgg aaagtcgatt aatcgtcttc ccgtagcagt
     5521 cctttttgta tgaacggctg ttatgatcac tcaacagctg ttataatcac tcaccgattt
     5581 ttatcttcgt ccaaaagatc gggttcgtcc gtagcgatgc cgttgacgcc catcgctttc
     5641 aaccgcaagg cggcttcggc gtcattcacc gtgtaagcat ataccgcgag acctttgtcg
     5701 tgcattgtgt tgacgaaagg aggcgtcaat gcttcgtagc tcacgttgat gcctgaagcg
     5761 tacgagagaa tatccttcaa ttccttcttc gttagtgccg cttcctcccc ttcgtcgaag
     5821 gaaaccaatt gaatcagcgg aatctcaggt tcaatctttt gcactttctt cagcgccttc
     5881 ttgtcgaatg aaagaatgac cgcttccgga tacccttccg cgtcgatacc gatcaactcc
     5941 tgctccttca tgagtcggac caattcctcc tcgatgccgg ggtattgctc tgtatctttc
     6001 aactcgatga caaagcggag gtctcctttc aactgttcaa ccacttcccg caatgtaggg
     6061 atttcatacg cttcttcgtt tctgccgctg gcggcgattt tcttgccttt cttctcatcc
     6121 gcggtcagct gtttcaattc ggccaatgta aagtccttta tcttccctgc cgcttctgtc
     6181 gtccgtttca cgtcatcgtc atgcatgacg atcaattccc catccttcgt caaatggacg
     6241 tcaagctcta cgtaatccgc attaaggtct tccgcaagct caaaggcagg aatcgtgttt
     6301 tccggttcgt atgccgaagc gccacgatgc ccgatgatca ggaagccttt ctcgggcatt
     6361 gggttgtccc agtcgtttcc gatgttgcag gcggacaata gaatcgccat actcatgatg
     6421 cataaaatct ttttcatact aaaatccttc caatgtaaaa agtccgctct cggcggactt
     6481 tttaaattta tttcgcaaac agatcggcaa tgacttcttc cacagccgct ttgccttggt
     6541 ctacgcagtc cggcagtccg acgccgtcat aagaggcgcc tgccaatttg acgttcggaa
     6601 actccctttt caattccgcg cgggcggcat cgatccgttg acggtgtccg acccggtatt
     6661 gcgggcggtc ctccttgaac cgtgtgacga ccgtgaagtc cggcttgccc ttgatcgtaa
     6721 tgattttact gaggtcctcg aggacgatcc gctcgatttc gtcatccggc aggtcgacga
     6781 ttgcgtcttc ccctacccgt ccgacaaacg cacgcagtag gactttccct tccggcgtcg
     6841 atgtcggcca tttccgatgc acccacgtgc atgcggtaat ggaataatcg ctgctccggg
     6901 aaacgaggaa gcctgtccct tccgtgtcct ggacgatatt ctcctcggga aaggcgaggg
     6961 cgatcgtcgc aaccgatgtt gtatggattt cattcagtcc ttgcagcagt ccgtgtgggg
     7021 cgaacagttg gctcgccgcc atatggcctg ttgtcaagat gacggagtcg gccacgatct
     7081 tttcacctgt attcagttcc aggacggttt gctccccctg tttggagatg ctttcgatgc
     7141 ggatcccttt ccgcaccgtt cccggttcca actgctgttc cgccgcttcg acgatcgact
     7201 caagcccgtt ccggagtgaa tggaacgccc cttttgggga agcgccatgt ccgtctttct
     7261 gcggcacttg cttcggcgtc gtcttcttca tgccgaggat gaggctgcgg tgtttctttt
     7321 ccacttcgta aaattgcgga aacgtcgatt gcaggctcat atggtcgata tcgcctgcat
     7381 acactcccga taatagcggc tcaatgaggt tttcgacgat ttccgcaccg aatcggcgac
     7441 ggaagaagtg gccgagtgac tggtcccctt caatccccga acgcggcaag acgaagtccc
     7501 ctgccgccct cagtttacct gccaatgaaa acaggtcgga tttcaggaac gggccgacct
     7561 tcgtcgggat gcccatgaca gagccgcccg gaatcggctg caatcggccg ttcatgagga
     7621 tatacgcctg tcccgtcgca tttcgcacga ggtcctcccc gatgccgacg tctttcgcga
     7681 acgtatccat gcttttcttc cggataagga agctgtccgg tccccgttcg atgacaaatc
     7741 cgtcacggcg gaccgtctgg attttcccgc ctagcctgtt cgtcgcttcg accagcgtca
     7801 cttcgatcgg caagttctgt tcacgggccg ctttttgcat atagaatgct gcggaaagcc
     7861 cagtgatccc tccaccgacg atgacgaccc ttttcttctt ttcactcatc tcatcacttc
     7921 ctagctgccc ttaggcttcc ttcaattgtt caaggactgc atccgccatc gcgttaatga
     7981 aaagcggatc cgtgttcggc attgccggac ggtaatagga tgcgccgatc tcatcgcaca
     8041 ccactttaca ctcatggtca ttgtcataaa ggacttccaa atggtccgaa acgaagccga
     8101 ctggtgtata aacgaatgtt tcatagcctt tctgctcatg caagtcacgc gtcaagtcct
     8161 ggacgtcagg cccgagccac ggttcgcccg tctgcccttc actttgccag ccgactgcgt
     8221 actcttcgac cccagccgct tccgcaatca atttcgcggt ctcttccaat tgctccggat
     8281 acggatctcc gaattgcttg atcttttcag gcaaggagtg cgccgatacg acgaggcacg
     8341 ccttttcacg ttctgcatcg ctcatgctgt catatgtgcc ccggatcttt tcggaccagt
     8401 attcgatgaa tttcggctgc ttgtaccaac tttcgacgga tgtcatggaa acgccgtgct
     8461 tctcggcttc ttccttcgcc cgttcattgt atgatttcac ggagaatgac gaataatgtg
     8521 gtgccaagac gatggacacc gcttctgtga tcccgtcggc ttccatctgt tccaccgctt
     8581 cttcgacaaa tggcgtaata tgtttcaaac cgacatagac gttgaattcg atttcatcct
     8641 gcatcgcatt cagacggtcg ccaagcgcgt tcgcctggtt ttccgtgatt ttcgcgagcg
     8701 gggaaattcc gccgatcgct tgataacgcg ccttcagatc ttccagttgt tccggtgcag
     8761 gcggacggcc gcgccggata tgcgtgtaat acggtaagat atcctcttcc ttgtatggcg
     8821 ttccgtacgc catgactaaa agtcccattt tcctttttgt catcagttgc acctcatttt
     8881 tcgttttcta cgcgatttat ttgcggagct gcgcgctgta ttcgtgaacg aattctgtca
     8941 atcgtttcaa tgttgccggc tggacttcag ggaagacgcc gtgtcctagg ttgaaaatgt
     9001 ggctgccgtg ctcgacacct tgttcaacga tgactttcgc acgctcttcg atgatgtccc
     9061 agtctgcgag aagcaatgca ggatccaagt ttccttgtaa tgtcttcgtc aatcctcttt
     9121 ccccggcttc cttgactgat aaacgccagt ccaagccgac gacat
//
LOCUS       DASULI010000011         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_388615, whole
            genome shotgun sequence.
ACCESSION   DASULI010000011 DASULI010000000
VERSION     DASULI010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388615"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="VFX34_00215"
     CDS             <1..1570
                     /locus_tag="VFX34_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5563351.1"
                     /translation="ANIAVHKAFGYRLKELTGRAIADLIGDEKVDNFRNFLSRAFSGE
                     SIEMEGIKFFHKRGQSLSAYIKSIPVTVRGDIQEIHLILRDTSFHHKNNERLLFLSYH
                     DQLTGLWNRRALKEHFEADAEYAFRSDEKLSLIHLDLDRFNTINDAIGRNGADEILKM
                     IAERIKMVIPKAAKLYRNGGDEYMILLKNHSIEKTEKLIQKILSDFTKPFYFNHQEYF
                     ISSSIGISVYPDDGVELEELLQKAEQAVRFIKERGRAHYRFYREEMNTAFTDTALMEA
                     HLRRAIEFNELELHYQPQVNLKTGEIDSFEALLRWKNRKFGFVSPSLFIPMAEESGLI
                     HTIGDWVLEVVCAQLKEWQDKQFRPVRIAVNISPKQFRTGAFAEKAKNIIEKHRIHPS
                     SFEVEITETALMNMKDTLSTLHKLKEIGVTISVDDFGTGYSSLSYLKQYPIDIIKIDR
                     SFIKDIELDERNEAIAKTIINLAHNLGMQVIAEGVEKDLQAKILKEADCHKAQGFLFS
                     KAVPASEIVEKYFA"
     gene            1700..>2120
                     /locus_tag="VFX34_00220"
     CDS             1700..>2120
                     /locus_tag="VFX34_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5563352.1"
                     /translation="MFAEKVIIVTGGSSGMGKFMAKKFADEGAYVIITGRDADRLEEA
                     RKEIGERAHTYQMDVRNIENVQGMVEFADEKFGKIDGLVNNAAGNFIVHAEELSPNGW
                     KSVIDIVLNGTFYCTSAVGNYWIKKGQKGSILNMLATY"
BASE COUNT          651 a          398 c          508 g          563 t
ORIGIN      
        1 tgcaaatatt gctgtacata aagcttttgg ctatcgtttg aaggagttga cgggtcgtgc
       61 cattgctgat ttgatcggtg atgagaaagt tgataacttc aggaatttcc tttcccgtgc
      121 attttcaggt gaatcaattg aaatggaagg gattaagttt ttccataaac gcggccaatc
      181 tttatctgca tacatcaagt cgatcccagt caccgtacga ggagatatac aagaaattca
      241 tttgattttg cgggatactt cctttcatca caaaaacaat gaaaggctat tattcctttc
      301 ctatcatgac caattgacag gtttatggaa taggcgggca ttgaaagaac actttgaagc
      361 tgatgctgag tacgccttcc gaagtgatga aaagctctcg ttgatccatc tggatttgga
      421 tcgtttcaat acgatcaatg atgcaatagg gagaaatggt gcagatgaaa tcctcaaaat
      481 gattgcagaa cgtataaaga tggttattcc gaaagcggca aagctgtatc ggaatggcgg
      541 ggatgaatat atgattttgc tgaaaaatca ctccatcgaa aaaacggaaa agctaatcca
      601 gaagatcttg agcgatttta caaaaccttt ctattttaat catcaagaat atttcatttc
      661 gtcctcgatc ggtatttcgg tgtaccctga tgatggcgtg gaattggagg aattgctgca
      721 aaaggctgag caagccgttc ggttcattaa agaacgtggc cgtgcccatt accgttttta
      781 ccgcgaagag atgaatacgg ctttcactga cacggcttta atggaagccc atttgcgccg
      841 cgcaatcgag ttcaatgagc ttgaactcca ctatcagccg caggtgaatt tgaagactgg
      901 tgaaattgac agctttgagg cgttgttaag gtggaaaaac cggaagttcg gcttcgtctc
      961 tccatcccta ttcataccga tggcggaaga gtcaggactt atccacacga tcggcgattg
     1021 ggtactggaa gtcgtatgtg cacaattgaa ggaatggcag gataagcagt tccggccagt
     1081 ccgtattgcg gtcaatattt ctccaaaaca attccggacc ggtgcattcg cagagaaagc
     1141 gaaaaacatc atcgaaaagc atcgaattca tccttcatca ttcgaggtgg aaataacgga
     1201 gactgctttg atgaatatga aagacacact gtcaacctta cacaagctga aggagatcgg
     1261 cgtgacgatt tccgtcgatg actttggtac ggggtactcc tcattaagct acttaaagca
     1321 atatccaatc gatattataa aaatcgatcg ctccttcatc aaagacattg aactggatga
     1381 aaggaatgag gcgattgcca aaacgatcat caatctggcc cacaaccttg ggatgcaagt
     1441 cattgcggaa ggtgtggaga aagatttgca ggctaaaatc ttaaaagaag ccgattgcca
     1501 taaggcgcaa ggttttttat tcagcaaagc ggttccagct tcggaaattg tggaaaaata
     1561 ttttgcgtaa tttttcttaa gacttccttc aacaggcaaa tcctgtgaga agggagtctt
     1621 tttgtaatag tggtcgttta atagatagct atgatacaat tatgagtgaa tattcattca
     1681 taagaatagg gggattagta tgtttgcaga gaaagtgatt atagtaacag gcggatcaag
     1741 cggaatgggc aaattcatgg cgaagaaatt cgccgacgaa ggcgcatatg tcatcattac
     1801 aggacgtgac gcggaccgtt tggaagaagc aaggaaagaa atcggggaac gcgcacatac
     1861 ttatcagatg gacgtgcgca acattgaaaa tgtacaggga atggttgaat ttgccgatga
     1921 aaagttcggc aaaatcgatg ggctcgtgaa caatgcagcc ggaaacttca ttgtccatgc
     1981 tgaagaactt tcgccgaatg gctggaagtc cgtcattgat atcgtgttga acggaacctt
     2041 ctattgcaca agcgctgtcg gaaattactg gattaaaaaa ggtcaaaaag gatccatttt
     2101 aaatatgctc gcaacatatg
//
LOCUS       DASULI010000012         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_738874, whole
            genome shotgun sequence.
ACCESSION   DASULI010000012 DASULI010000000
VERSION     DASULI010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738874"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFX34_00225"
     CDS             <1..392
                     /locus_tag="VFX34_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563353.1"
                     /translation="FIGTLLAIFIIFINSTIGSMGGEKGKGEESRAEAQLEQALMKIE
                     GIGEVFIHFHYEQKEEPSPLSGYFSGSGSSSVKKNPLQGVLVIAEGADNPRTKNELTR
                     ILSTVLQLPEHRIVVEEMKRGIHVESE"
     gene            379..921
                     /locus_tag="VFX34_00230"
     CDS             379..921
                     /locus_tag="VFX34_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HEX5563354.1"
                     /translation="MKANKRTVWFLTLLSLVAVISIYYLKKEAPMSLDGMAIFGDKDA
                     VTVSKPGDVEGETKTVYADSMLFEEMRMKVLDERSTLKDQLLSKVQSPDTTAEEKSAA
                     YDEMAELTKRTSAEDLMETQLKALGYPEAFVRKDDGKVSVSVISEDGGHSTKMATEIM
                     QYILTSWEDARTVQVDFMEK"
     gene            1063..1581
                     /gene="accB"
                     /locus_tag="VFX34_00235"
     CDS             1063..1581
                     /gene="accB"
                     /locus_tag="VFX34_00235"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5563355.1"
                     /translation="MLKIQEIREIIKLIDQSSIEKFTFESEGTKIKLEKANGVQQHPV
                     QVEQKKEEPVQHVQPAVQEAKKEQTEHAPAKQESKEPSETSSAAENDPSLHKITSPMV
                     GTFYQSSSPDADPYVQKGDSVKSDSIVCIVEAMKLFNEIEAEVSGEIVEILVEDGQLV
                     EYGQPLFLVKQK"
     gene            1596..2942
                     /gene="accC"
                     /locus_tag="VFX34_00240"
     CDS             1596..2942
                     /gene="accC"
                     /locus_tag="VFX34_00240"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5563356.1"
                     /translation="MKKVLIANRGEIAVRIIRACRELDIQTVAVYSEADREALHVELA
                     DEAYCIGPKLSKDSYLNFSNIISIAKLTGCDGIHPGYGFLAENASFAELCEEVNIEFI
                     GPTADAISRMGTKDVARETMKQAGVPIVPGSDGIVADEIEGLKVAKEIGFPVIIKATA
                     GGGGKGIRVARNEEELAKGIKITQKEAAAAFGNPGVYLEKYIEEFRHVEVQVLADKHG
                     NTIHLGERDCSIQRRMQKLVEEAPSPALTPKLRERMGEAAVKAAEAVNYRGAGTVEFI
                     FDHINQEFYFMEMNTRIQVEHPVTEMITGIDLIQQQLKVAAGEKLAYRQKDIKINGWA
                     IECRINAENPAKNFMPSPGKVTMYMPPGGFGVRVDSAMYTGYSIPPFYDSMVAKLIVH
                     ADTREEAVARMKRALDEFIIEGVDTTIPFHLNLMDHEVFKSGDFDTKFLEKYTVMK"
     gene            3028..3429
                     /locus_tag="VFX34_00245"
     CDS             3028..3429
                     /locus_tag="VFX34_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX5563357.1"
                     /translation="MADKANPSFAGKSSKGDEVLGRVQLAPEVLEVIIGIATNEVEGV
                     ATTKGNFATGVAEKFGKVFHGKGVKTDWTGDKLFIDVYCVVQYGHSIPNVATEIQRQI
                     RHAVYNMTSLETEEVNVHITGIDFDTDPESE"
BASE COUNT         1099 a          695 c          901 g          824 t
ORIGIN      
        1 tcttcatcgg gacgctgctc gcgattttca tcattttcat caattcgacg attggttcga
       61 tgggagggga gaaagggaaa ggggaggaga gccgggccga agctcaactt gaacaagcat
      121 tgatgaagat agaagggatt ggggaagtgt tcatccactt ccattacgaa caaaaagaag
      181 agccaagccc attatccgga tatttttcag gctccggctc ttcatcggta aaaaagaatc
      241 cgctccaagg agttttagtc attgcagaag gggcggacaa tcctagaaca aaaaacgaat
      301 tgacacgaat cttgtcgacg gttcttcaat tgcctgaaca tcggatagtc gtcgaagaaa
      361 tgaaaagggg gatacatgtt gaaagcgaat aaacgtacag tttggttctt gactttacta
      421 agtctagttg cggtcatctc gatctactat ttgaaaaagg aagcaccaat gtctttagat
      481 ggaatggcga ttttcggaga taaagatgcg gtgaccgtat cgaagcccgg ggatgttgaa
      541 ggcgaaacga aaaccgtcta tgcagattca atgctcttcg aagaaatgag aatgaaagtc
      601 cttgatgaaa gaagcacgtt gaaggatcaa ttattatcca aagttcaatc tcccgataca
      661 accgcagaag aaaaaagcgc ggcatatgat gaaatggcgg aactcaccaa acgtacatct
      721 gctgaagatt tgatggaaac acaattgaag gctttaggct atccggaagc attcgtccgt
      781 aaagatgacg gtaaagtgag cgtttctgta atttcagaag acggcggaca ttccacaaag
      841 atggcgacgg aaattatgca gtatatatta acgagctggg aagatgcacg tactgtccaa
      901 gtcgacttta tggaaaaatg attaaatgag gccctaaatg ggtctttttc tttttattta
      961 gccgtatttc acagtaaatg cagtatatct atttcaatat ggactaaaaa cgtctaaaat
     1021 agggaggagg tacgtaaata aaaaagagtc aggagaattg tcatgttgaa aattcaagaa
     1081 atccgtgaaa tcattaaatt aatcgatcaa tcttcaattg aaaaattcac ttttgaatca
     1141 gaaggaacaa aaattaaact tgaaaaagct aacggtgttc aacaacaccc cgtacaagtt
     1201 gagcaaaaga aagaggagcc cgttcagcat gtacagcccg cagtccaaga ggcgaagaaa
     1261 gaacagaccg agcatgcgcc tgcaaaacag gagagcaaag agccttctga aacttcttct
     1321 gcagcagaga acgatccatc attgcataaa atcacatcgc ctatggtcgg cacattctac
     1381 cagtcttcat cgccggacgc ggatccgtat gtacaaaaag gcgactccgt taaatcggat
     1441 tcaatcgttt gcattgtgga ggcaatgaag ctcttcaatg aaatcgaagc ggaagtttcc
     1501 ggagaaatcg tggagatcct agtggaagac ggtcaattgg tcgaatatgg acagcctctc
     1561 ttccttgtca aacagaagtg agggggaatg caaaaatgaa aaaggtatta atagccaacc
     1621 ggggcgaaat agcagtaaga atcatccggg catgcaggga attggatatt caaacggtcg
     1681 ccgtctattc ggaagcggat cgggaggcgc ttcatgtgga gcttgccgat gaagcgtatt
     1741 gcatcggacc gaaattatcc aaagacagct atttgaattt ttccaatatc atcagcatcg
     1801 caaaattgac tggatgtgac ggaatccatc caggctatgg cttccttgct gaaaacgcca
     1861 gctttgcgga gctttgtgaa gaagtgaata tcgaattcat cggtccgact gccgatgcga
     1921 tttcgaggat gggaacgaaa gacgtagcaa gagaaacgat gaaacaggcg ggcgtgccga
     1981 tcgttcctgg atccgacggg attgttgcag atgaaatcga aggactgaaa gtggcgaagg
     2041 agataggttt cccggtcatc atcaaagcta cagccggcgg cggcggcaaa gggatccgtg
     2101 tcgccagaaa tgaagaggaa cttgccaaag gcatcaaaat cacgcagaag gaagccgcag
     2161 cagcattcgg caatcctggt gtatatcttg aaaagtacat cgaagagttc aggcacgttg
     2221 aggtgcaagt ccttgcggat aagcatggca atacgattca tttaggtgaa cgtgattgtt
     2281 ccattcaaag aaggatgcaa aagcttgtag aggaagcgcc ttcgccggct ctgacgccga
     2341 aactgcgcga aagaatgggt gaagctgctg tcaaggctgc agaagctgta aactacagag
     2401 gcgccgggac agtcgagttc attttcgatc atattaatca ggaattttac tttatggaaa
     2461 tgaacacccg aattcaggtt gaacatccgg taaccgagat gatcacaggc atcgatctca
     2521 ttcagcaaca attgaaagtt gccgcgggtg aaaagcttgc ttaccgtcaa aaggatatta
     2581 agattaacgg atgggcgatc gaatgccgga tcaatgcaga aaaccctgcc aagaacttca
     2641 tgccttctcc ggggaaagtg actatgtata tgccgccggg cggatttggg gtcagggtcg
     2701 attcagctat gtatacgggc tattcgattc ctccgttcta cgattccatg gtggcgaaac
     2761 ttatcgttca tgcggataca cgcgaggaag cagttgcaag aatgaagcgc gcactcgatg
     2821 aattcattat tgaaggggtc gatacgacga ttcctttcca tctgaatttg atggaccatg
     2881 aagtgttcaa gtcaggcgac ttcgatacga agttccttga aaaatatact gtcatgaagt
     2941 aagttggcta atttggcata aaattattgt gaatgcaagc aacaaattgg ccggaatgaa
     3001 atgcatatga gagggaggag aagtggaatg gctgataagg caaatccttc attcgcggga
     3061 aagagttcaa agggagacga ggttttaggc cgcgttcaat tggctcctga agtgcttgag
     3121 gtcatcatcg gcattgcaac gaacgaagtc gaaggcgtcg ctacgacaaa aggcaacttc
     3181 gcaactggtg ttgcggagaa gttcggcaaa gtgttccatg gaaaaggggt caagacggat
     3241 tggactggcg ataaactctt catcgatgtc tattgcgtcg tccaatatgg ccactcgatc
     3301 ccgaatgtag cgacagagat tcaaagacag atccgtcacg ctgtctacaa catgacttct
     3361 ctggaaacgg aagaagtgaa tgtccatata accggcattg atttcgatac agacccggaa
     3421 tcagaatgat ctgaaaagca tcgtccattg gatggtgctt ttcaacttgt cctgctatat
     3481 cttcgtcaac tgaatatgct atgataaagc ccaagagac
//
LOCUS       DASULI010000013         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_718969, whole
            genome shotgun sequence.
ACCESSION   DASULI010000013 DASULI010000000
VERSION     DASULI010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718969"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFX34_00250"
     CDS             complement(<1..319)
                     /locus_tag="VFX34_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEX5563358.1"
                     /translation="MKNSFKTKIERLNRIDTFSPYFFLPFILVLYFFTSLFDFHRFEL
                     FNVRVSIWPAVFIAAISYYVGVYLIDKKGWTFPTFGLSFLRGQTTLFIWLLAIIGGVA
                     YITM"
     gene            complement(332..1858)
                     /gene="murJ"
                     /locus_tag="VFX34_00255"
     CDS             complement(332..1858)
                     /gene="murJ"
                     /locus_tag="VFX34_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX5563359.1"
                     /translation="MNKLKIASVLFLLSTFVLKFSSMIRDLVIAGLFADSYRADVYFA
                     AMTIPNAFVLFLLTGMKDAFLPSYYKHSALGKGEEHLTNIVKGTFLISSAFAVVGAAL
                     SYPLVQIVYPKFADYADGFQIASWTVAIYFLSLIFVGINSVYEGYFDAKKKFSFSVFS
                     QTSVVLITILFALLFHSRWGILAVPIGYFVGTVISLLIKIVYRKPRTFMNWTQKIDRE
                     EVKEFYGIFWPVGLTIAVGQINLMVNTYFAAGLGEGVVSNLNYAFRLVNIPQAIFGVT
                     IATIAFPIIAQARAENNMQKFRKGIEKGLLYLLVFLTPMLAGMWLLMDELVEVVYQRG
                     KFTESATIITASYAVFYIGSAFFYSIQAVVAKGFYTLEKGQYMMRIGLISIVLNIISN
                     AIFSKIYGAQGIALSASLVALLYCAITFTTLWKLIGGMDRSYLLKNTIQVILATAAMT
                     GVLIVADKYTEIADLPALLHILTIALPGVFIYFIVLMVFRNEFVKTILSKGGNTSSLP
                     "
     gene            complement(1851..2588)
                     /locus_tag="VFX34_00260"
     CDS             complement(1851..2588)
                     /locus_tag="VFX34_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX5563360.1"
                     /translation="MKESMFGVNVNTETSDELMGLIFSRLEQGEKSFLVAINPEKLMK
                     ARNDAGLKKILNTATFQIPDGIGVVLASRLRKGGVTGRITGIDMMENLCREAAERNKS
                     VFLYGAKPGVAEKAASELQKKFPSLQVAGTLDGYVKDENEIIKTINEANADILFVALG
                     SPTQENFIIRNMSKLTVSVFQGVGGSFDVFSGNIKRAPAGFRKLGLEWLYRLLLEPSR
                     LKRQLELPKFLLAILTERQSTGGKLNE"
     gene            complement(2622..>3187)
                     /locus_tag="VFX34_00265"
     CDS             complement(2622..>3187)
                     /locus_tag="VFX34_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX5563361.1"
                     /translation="ANFHPRVNIHAPGPGVGGHCIAVDPWFIVELQPELSKIIKLSRT
                     TNNSMPEYVAEQAQKLMMEYTTGGKKAAVFGLSFKGNVDDMRESPAVTLIEELQKRKI
                     EVASYDPHIKPGGHPTQKDSVEEALEDAEILLIVTDHNEFKNLDPNVVAQQMKSKIVY
                     DTKNILDRSIWKAAGFNHHLLGDSKEK"
BASE COUNT          880 a          724 c          721 g          862 t
ORIGIN      
        1 tcatcgtaat ataggcaact ccaccgatga tggcaaggag ccaaatgaat agtgttgttt
       61 gtcctcggag gaatgatagc ccaaacgtcg ggaatgtcca tcctttctta tcgatgaggt
      121 agaccccaac gtaatagcta atggctgcta tgaaaacggc cggccaaatg gaaacgcgga
      181 cattaaacaa ttcaaatcga tggaagtcaa atagacttgt aaagaaataa agcaccaaga
      241 taaaaggcaa aaagaaataa ggtgaaaatg tatcaatgcg gttcagccgc tcgattttcg
      301 tcttaaatga atttttcata agcgtagtcc atcatggaag tgatgatgta ttccctcctt
      361 tggataagat cgtcttcacg aattcattcc tgaataccat gaggactatg aaataaataa
      421 atactcctgg taaagcaatc gttaatatat gaagcaatgc cggtaaatcg gcaatttccg
      481 tatatttgtc tgcgacaatg agaacgcccg tcatggcagc tgtcgctagt atgacttgga
      541 ttgtgttctt caataaatac gaacgatcca tgccgccaat tagtttccat aacgtggtga
      601 aagttatggc acagtagagc aaggcaacga gtgaagcaga aagcgcaatc ccttgtgcac
      661 cgtatatctt ggaaaagatg gcattggaga tgatgttaag aacgattgat atcagtccga
      721 tgcgcatcat atattgccct ttttccaatg tatagaaccc ttttgctaca acagcttgga
      781 ttgaatagaa aaaggccgat ccaatgtaga atactgcata ggatgccgta atgatggtgg
      841 cactttccgt aaacttaccg cgttggtaga caacttcgac aagttcgtcc atcaatagcc
      901 acattccagc aagcatcggt gtcaggaata ccaacaaata gagaagcccc ttttcaatcc
      961 cttttcggaa cttctgcata ttgttctccg ctcgtgcttg cgcaataatt gggaacgcaa
     1021 tggtcgcaat ggtcacccca aagattgcct gtggaatatt gacgagacgg aacgcatagt
     1081 tcaggttgga gacgactccc tcaccaagtc cagcggcgaa gtacgtattg accatcaggt
     1141 tgatttgtcc tacggcaatc gtgagaccaa ccggccagaa gataccgtag aattccttga
     1201 cctcttccct gtcaatcttc tgcgtccagt tcataaacgt cctcggcttc cgatagacga
     1261 ttttaataag aagcgatatg actgttccga caaaatagcc gatcggaacg gctaagatcc
     1321 cccatcttga atggaataaa agagcgaaaa ggatcgttat gagcacaacg cttgtttgag
     1381 agaagacaga gaatgaaaac ttcttcttcg cgtcaaagta tccttcgtaa acagagttga
     1441 ttcctacaaa tatgagggac aaaaagtaga ttgctactgt ccaggatgca atttgaaatc
     1501 cgtccgcata gtcggcaaac tttggataga cgatttggac gagtgggtag gacagggctg
     1561 ctcctactac tgcgaaggca gatgaaatca aaaaggttcc tttaacgata ttcgttaggt
     1621 gttcttcccc tttgcccaat gcagagtgtt tatagtaact cggcagaaac gcatctttca
     1681 ttcctgttaa tagaaagagg acaaacgcat tcggtattgt cattgcggca aaatacacat
     1741 ctgcacgata cgagtcagcg aacaatcctg caatgacgag gtcgcggatc atactcgaaa
     1801 atttcaggac gaacgttgac agcaaaaaca ataccgaagc gattttgagt ttattcattc
     1861 aacttcccgc cagtggattg gcgctccgtc agaattgcca agaggaactt cggcaattca
     1921 agttgacgct tcaatcggga tggctcaagc aacaaccgat agagccattc caacccgagt
     1981 ttcctaaatc cggcaggcgc ccgtttgatg ttacctgaga agacatcgaa tgatccgccg
     2041 acgccttgga acacagagac ggtaagcttg gacatgttgc ggataataaa attctcttgc
     2101 gtcgggctgc cgagcgctac gaagagaata tccgcatttg cctcgtttat cgtcttgata
     2161 atttcatttt cgtcctttac atagccatca agcgtacctg cgacttgcaa ggaaggaaat
     2221 ttcttttgca gctctgacgc tgctttttcg gcaactcctg gtttagctcc atacaggaat
     2281 acggacttgt tccgttcggc agcctccctg cacaggtttt ccatcatgtc gatccctgtg
     2341 atccgaccag tgacgccacc cttgcgcaat cgtgatgcaa ggacgacacc aatgccgtcc
     2401 ggaatttgga acgtagctgt atttaggatt tttttcagtc ccgcatcgtt tcgcgctttc
     2461 atgagttttt ccggattaat cgcgacaagg aaagattttt caccttgttc aagacgtgaa
     2521 aaaatgagtc ccattaattc gtcggacgtt tccgtattga cgtttacacc aaacatgctt
     2581 tctttcatat cggatcacct aagtaagtcg attactcgat tttatttttc tttgctatca
     2641 ccaagtagat gatgattgaa tcctgccgcc ttccaaatgg aacggtcgag gatgttcttt
     2701 gtatcataga caattttgct tttcatttgc tgagcgacca cgttcggatc aaggttcttg
     2761 aactcgttgt ggtcggttac gatgaggaga atctccgcat cttcaagcgc ttcctctacg
     2821 gaatcctttt gcgtcgggtg accacccggc ttaatgtgtg gatcgtagga agcaacttcg
     2881 attttgcgct tttgcagctc ttcaatcaat gttaccgctg ggctttcacg catgtcatcg
     2941 acgttccctt tgaatgaaag tccgaagact gcagcctttt tgccgcccgt tgtatattcc
     3001 atcataagct tttgcgcctg ctcggcaacg tattccggca tggaattgtt cgttgtacga
     3061 gagagtttga taattttcga taattccggt tgaagttcaa caatgaacca tgggtcaacc
     3121 gcgatacagt gaccgccgac gccaggtccc ggcgcatgga tgttcacgcg tgggtggaag
     3181 ttggcga
//
LOCUS       DASULI010000014         5295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_154882, whole
            genome shotgun sequence.
ACCESSION   DASULI010000014 DASULI010000000
VERSION     DASULI010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154882"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /gene="atpE"
                     /locus_tag="VFX34_00270"
     CDS             <1..156
                     /gene="atpE"
                     /locus_tag="VFX34_00270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5563362.1"
                     /translation="GNGLIVSKTVEGMARQPEARGMLQTTMFIGVALVEALPIIATVI
                     AFIVMNK"
     gene            346..876
                     /gene="atpF"
                     /locus_tag="VFX34_00275"
     CDS             346..876
                     /gene="atpF"
                     /locus_tag="VFX34_00275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5563363.1"
                     /translation="MSASGGGFLSSLNDPNGLNLGDIIVTVLFFTILMVLLKKFAWGP
                     LMGVMEQRAELIANEIEAAEKSRLESQKLLEEQRALLKEARDNAQSIVENARKQGETQ
                     REELMAAARAEVNRMKESATLEIATEKEKAVAAVREEFVSLSILAASKVLGKEISEED
                     NRALIEETIVKAGESR"
     gene            873..1412
                     /locus_tag="VFX34_00280"
     CDS             873..1412
                     /locus_tag="VFX34_00280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX5563364.1"
                     /translation="MSNSVVAKRYASALFELAQQHGQTGSIQEEMKELKKAFRDNKDL
                     GELFGSPKLSSAKKKEMIANIFKGANPLVLNALYVLLDAKRMEEAQNVFEEFLALADD
                     AAGIAEAKVYSTRPLTEQETNGISATFAKKIGKQSLRIENIIDPSLIGGVRLQIGNQI
                     YDSSLSAKLDKLQRKLIGS"
     gene            1436..2947
                     /gene="atpA"
                     /locus_tag="VFX34_00285"
     CDS             1436..2947
                     /gene="atpA"
                     /locus_tag="VFX34_00285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5563365.1"
                     /translation="MSIKAEEISTLIKQQIENYQSEMEVSEVGTVIKIGDGIALAHGL
                     DNVMAGELLEFSTGVMGMAQNLEANNVGIVILGPYTDIKEGDEVRRTGRIMEVPVGEE
                     LIGRVVNPIGLPVDGLGPIATTKTRPIESPAQGVMARKSVHEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTVAIDTILNQADQDMICIYVAIGQKESTVRGVVETLRKNGAL
                     DYTIVVTASASQPAPLLFLAPYTGVTMAEEFMFQGKHVLVVYDDLSKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDTLGAGSITALPFVETQAGDISAYIPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGLSVSRVGGSAQIKAMKKVAGTLRLDLAAYREL
                     EAFAAFGSDLDAATKAKLDRGARTVEVLKQDLNKPLKVEYQVVILYALTRGLLDDVAV
                     QDVLRFESELTSWLESNHTEVYDHIRTTKDLPSDEVMKDAINAFKKNFVPSES"
     gene            3004..3861
                     /gene="atpG"
                     /locus_tag="VFX34_00290"
     CDS             3004..3861
                     /gene="atpG"
                     /locus_tag="VFX34_00290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX5563366.1"
                     /translation="MVSLRDIENRIKSTKKTSQITKAMQMVSASKLNRAEMNARAFVP
                     YMDKIEEVVGAIASATTDSGHPMLVNRPVKKTGYIIVSSDRGLVGGYNANIFRAARRA
                     IEQRHTSKDDVKIVAIGRKGLEFFRRLGYEVVESLVGVSDHPSFDEIKAIANRAVGMF
                     TEGEFDEVYLYYSHYISAISSEVTEKKLLPLTDLSSASALTSYEFEPSAEAILETLLP
                     QYAESLIYGAVLDGKASEHASSMTAMKTATDNAGELIDSLSLQFNRARQAAITQEITE
                     IVGGVAALE"
     gene            3900..>5295
                     /gene="atpD"
                     /locus_tag="VFX34_00295"
     CDS             3900..>5295
                     /gene="atpD"
                     /locus_tag="VFX34_00295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5563367.1"
                     /translation="MSNGQILQVMGPVVDVKFENGQLPEIYNALKVQIQRPNAEAETL
                     TLEVALHLGDDSVRTIAMSSTDGLQRGAVVTDTGAAISVPVGDVTLGRVFNVLGEIID
                     LGEEVPAAERRDPIHRLAPKFENLSTKVEILETGIKVVDLLAPYIKGGKIGLFGGAGV
                     GKTVLIQALINNIAQEHGGISVFAGVGERTREGNDLFYEMTDSGVINKTAMVFGQMNE
                     PPGARMRVALTGLTMAEYFRDEQGADVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQ
                     PTLATEMGQLQERITSTNVGSVTSIQAIYVPADDYTDPAPATTFAHLDATTNLERKLS
                     EMGIYPAVDPLASTSRALSPEIVGEEHYEVARQVQTTLQRYRELQDIIAILGMDELSE
                     EDKLVVGRARRIQFFLSQNFHVAEQFTGQKGSYVPVAETVQGFKEILEGRYDHLPEDA
                     FRLVGRIEEVIEKAK"
BASE COUNT         1500 a         1258 c         1308 g         1229 t
ORIGIN      
        1 ggtaacggtc ttatcgtttc aaaaacagtt gaaggtatgg cgcgtcaacc agaagctcgc
       61 ggtatgcttc aaacaacaat gttcatcggg gtagcgttag ttgaggccct tccgatcatc
      121 gcgacagtta tcgcgttcat cgtcatgaac aaataataaa acaattttaa atggcgaaga
      181 acccggcaga actgcccttc gccattcatt tatgtaacaa ctgacgtttg aaatcgtact
      241 ttgaagttga aaattaagaa tgacgttctt tctatattat attgaaaatt gacatgtgct
      301 cttgaaggga gtgaaacaat cgtgttagga aacaccttca tcctattgtc cgcaagtggc
      361 ggaggatttc tgtcatcatt gaatgacccg aatggcttga atctaggcga catcatcgtc
      421 acagtattat tcttcacgat cctcatggta ctactcaaga agttcgcatg gggcccgctt
      481 atgggcgtga tggaacaacg tgcagaattg atcgcaaatg aaatcgaagc agctgaaaaa
      541 agccgcttgg aatctcaaaa gcttttggaa gaacagcgtg cccttttaaa ggaagcgcgt
      601 gacaacgcac aatcgattgt tgaaaatgct cgtaagcaag gtgaaacaca acgtgaagag
      661 ctgatggcag cagcacgtgc ggaagtgaac cggatgaaag agtccgctac acttgaaatc
      721 gcgacagaga aggaaaaggc agttgcagct gtccgcgaag aattcgtttc actttcaatc
      781 ctggcggcgt cgaaagtact tggtaaggaa atctccgagg aagacaaccg tgctttgatt
      841 gaagaaacga ttgtgaaggc aggcgaaagc cgatgagtaa ttcggtcgta gcaaaacgct
      901 atgcatccgc attgttcgaa ctagctcaac aacatggaca aaccggttcg attcaggaag
      961 aaatgaaaga attgaagaag gcgttccgtg acaataaaga ccttggtgaa ctattcggtt
     1021 ctccgaagtt atcatcagcg aagaagaagg aaatgatcgc aaacattttc aaaggcgcga
     1081 atccgctcgt attgaatgca ctgtatgttc tgcttgatgc gaagcgcatg gaggaagcgc
     1141 aaaatgtctt cgaagaattc cttgcactag cggatgatgc tgcgggaatc gcagaagcga
     1201 aagtgtactc gacgcgcccg ttgacggagc aagagacgaa tggcatttcc gccactttcg
     1261 caaaaaagat cggcaaacaa tctttacgca ttgaaaatat catcgatcca agcctgatcg
     1321 ggggcgtccg ccttcaaatc ggcaatcaga tttacgacag cagcttgagc gcgaagctcg
     1381 acaaactgca acgtaagctg atcggatcct aatttgaaat tgaggggtga catacatgag
     1441 catcaaagct gaagaaatca gcacgctgat aaagcagcag attgaaaact atcagtctga
     1501 gatggaagta agcgaagtcg gtacggttat caaaatcggt gacggtatcg ctcttgctca
     1561 tggcctcgac aacgtcatgg ccggagagct tcttgagttc tccacaggtg tcatgggtat
     1621 ggcgcaaaac ttggaagcga acaacgtagg tatcgttatc ctcggaccat acacagatat
     1681 caaagaaggc gatgaagtac gtcgtactgg ccgtattatg gaagttcctg tcggtgaaga
     1741 attgattgga cgcgtcgtaa atccaatcgg cttgccggta gatggattgg gcccgatcgc
     1801 aacaacgaaa acccgtccaa tcgaaagtcc tgcacaaggc gtcatggcgc gtaaatccgt
     1861 tcatgagcca ttgcaaacag gcatcaaggc gatcgatgca cttgtaccga tcggccgtgg
     1921 acagcgtgaa ttgatcatcg gtgaccgtca aacaggtaaa acgactgttg caatcgatac
     1981 aattcttaac caagctgacc aagatatgat ttgtatctat gttgctatcg gacaaaagga
     2041 atccacagtt cgtggggttg ttgaaacact ccgtaaaaac ggtgcgcttg actacacgat
     2101 cgtagttaca gcatccgctt cacaaccggc tccattgcta ttcctagcac cttatacagg
     2161 tgtaacgatg gctgaagaat tcatgttcca aggcaagcac gtcctcgttg tttacgatga
     2221 cctatctaaa caagcggctg cttaccgtga actttccttg ctacttcgcc gtcctccagg
     2281 ccgtgaagct taccctggtg acgtattcta cttgcacagc cgtctactcg agcgtgcagc
     2341 gaagttgaac gatacattgg gcgcaggttc aatcactgca cttccattcg ttgaaacaca
     2401 agccggggac atctccgctt acattccaac gaacgtaatt tccatcacgg atggacagat
     2461 cttcttgcag tcggatctct tcttctcggg tgtacgtcca gcgatcaacg ccggtctatc
     2521 cgtatcccgt gtaggtggtt ccgcgcaaat caaagcgatg aaaaaggttg cgggtacact
     2581 ccgtcttgac cttgcagcat accgtgagct tgaagctttc gcagcgttcg gttccgacct
     2641 tgacgcagcg acaaaagcta aacttgaccg cggtgcgcgc actgttgaag tcttgaaaca
     2701 agacttgaac aagccgctga aagtcgaata ccaagtagtc atcctttatg cattgactcg
     2761 cggacttctc gacgacgtag ccgtgcaaga cgtccttcgc ttcgaaagcg aattgacgag
     2821 ctggctcgaa tccaaccaca ctgaagtgta tgatcatatc cgtacgacaa aagaccttcc
     2881 gtctgatgaa gtgatgaaag atgcgatcaa cgcattcaag aagaacttcg taccgtctga
     2941 gtcttaattc gaaacgtatc aaaggatttt gatctaacta aacaataagg tggtgaattg
     3001 ccagtggtat cattacgcga tattgaaaac cgtattaaat cgacaaagaa gacgagtcag
     3061 attacgaaag cgatgcaaat ggtgtcggct tccaaattga atcgtgcaga gatgaatgcg
     3121 agagcgttcg tcccttacat ggataaaatc gaggaagtcg tcggcgccat cgcaagcgct
     3181 acaaccgact ccggacaccc aatgctcgta aaccgtccag tgaagaagac cggttatatt
     3241 atcgtatctt ccgaccgcgg tctcgtaggg ggttacaatg cgaacatctt ccgtgccgcc
     3301 agacgtgcga tcgagcagcg ccatacatcc aaggacgacg tgaaaatcgt cgccatcgga
     3361 cgcaaagggc tagagttttt cagacgcctc ggatacgagg tcgttgaaag tctcgtcggc
     3421 gtatcggacc atccttcttt cgatgaaata aaagcaatcg cgaaccgagc tgttggcatg
     3481 ttcacagaag gcgaattcga tgaagtgtac ttgtactaca gtcactacat ctccgccatc
     3541 tccagtgaag tgacggaaaa gaaattactt ccgctcacgg acctttcatc agcgtcagca
     3601 ttgacttcgt acgagtttga gccttccgcg gaagcgattc ttgaaacgct tcttccgcaa
     3661 tacgcggaaa gcctcatcta cggggcagtg cttgatggaa aagcgagcga acatgcttcc
     3721 agtatgacag cgatgaagac tgctacagat aacgcaggcg aattgatcga ttcattgagc
     3781 cttcaattca accgtgcacg tcaagcagca attacacaag aaatcactga aatcgtcggc
     3841 ggagttgccg ctcttgaata aagcaatttg aacgggaaag taagacagga gggaaaagca
     3901 tgagtaacgg acaaatactt caagttatgg gtccggtcgt tgacgttaaa ttcgaaaacg
     3961 gtcagcttcc agaaatctat aatgctttga aggtccaaat ccaacgtccg aacgcggaag
     4021 cagaaacgtt gacacttgaa gtcgcactcc accttgggga cgactcggtg cgcacgattg
     4081 caatgtcatc cacagatggc cttcaacgtg gcgctgtcgt tacagacaca ggcgcagcga
     4141 tttcggttcc ggtcggcgac gtgacacttg gacgtgtatt caacgtactt ggagaaatca
     4201 tcgaccttgg agaggaagtt ccggcagcag aacgccgcga cccgatccac cgtctggctc
     4261 caaaattcga aaacctttca acgaaagttg aaatccttga aacaggtatt aaagttgtcg
     4321 acttgcttgc accatacatc aaaggtggta aaatcggcct cttcggtggt gccggtgtag
     4381 gtaagaccgt tcttatccag gcattgatca acaacatcgc acaagaacac ggcggtatct
     4441 ccgtattcgc aggtgttggt gagcgtacac gggaagggaa cgacttattc tatgaaatga
     4501 cggattccgg cgttatcaac aaaacggcga tggtattcgg acaaatgaac gagccgcctg
     4561 gcgcacgtat gcgtgttgcg ttgactggtt tgacaatggc tgaatacttc cgtgacgaac
     4621 aaggcgcgga cgttctattg ttcattgaca acattttccg cttcactcaa gcaggttctg
     4681 aagtatccgc acttcttggc cgtatgccat ctgccgttgg ttaccaaccg acgcttgcaa
     4741 ctgagatggg tcagcttcaa gagcgtatca cttcaacaaa cgtaggatcc gttacatcga
     4801 tccaagcaat ctacgtaccg gccgatgact atacggaccc ggctccggct acgacattcg
     4861 ctcacttgga cgctacaacg aaccttgagc gtaaactatc tgaaatgggt atctaccctg
     4921 cggttgatcc gcttgcttcc acttcacgcg cactaagccc ggaaattgtc ggcgaagagc
     4981 attacgaagt ggcacgtcaa gtacaaacga cgttgcaacg ttaccgtgag cttcaagata
     5041 tcatcgcgat cctcggtatg gatgagctta gcgaagaaga caaacttgtc gtaggacgcg
     5101 cacgccgcat ccaattcttc ctatcgcaaa acttccacgt tgccgagcag ttcacaggcc
     5161 aaaaaggttc ttacgttcct gtggctgaaa cggttcaagg cttcaaggaa attttggaag
     5221 gccgctacga ccaccttccg gaagatgcat tccgcctcgt cggacgcatc gaagaagtta
     5281 tcgaaaaagc aaaag
//
LOCUS       DASULI010000015         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_660710, whole
            genome shotgun sequence.
ACCESSION   DASULI010000015 DASULI010000000
VERSION     DASULI010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660710"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFX34_00300"
     CDS             complement(<1..222)
                     /locus_tag="VFX34_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5563368.1"
                     /translation="MKISYHGHSVVKIETEGKTLLFDPFITGNELTDLEASEQTPDVI
                     LLTHGHNDHVGDTFDIAKRCNPLIVAPNEL"
     gene            325..1422
                     /locus_tag="VFX34_00305"
     CDS             325..1422
                     /locus_tag="VFX34_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5563369.1"
                     /translation="MDKINEIKDFLRKYDVDAAFITTPDNVFYVSGFRSEPHERLLGV
                     MIFKDAEPFVICPLMEVPDLKASGWTFEAVGHQDTEDAWEVVAAAAAKRGTPLQTIAI
                     EKSHLTVERLERMEQLFEGATFQRLDEQLNNMRNIKSEDELEKLRKAAALADHAIEIG
                     CKEIAEGKTELEILMAIEFEMKKMGAEKMSFDTMVLSGPKTASPHGTPGDRKIQKGDF
                     ILFDLGVVYEGYCSDITRTVAFGEPSEKQREIYETVKKAEQTAIDMIRPGVKACDLDK
                     ASRDVITEAGYGEYFTHRLGHGLGISVHEFPSITGTNDMELVEGMVFTVEPGIYHPEI
                     TGVRIEDDVVVTSDGVEVLTKFPKELQIIKP"
     gene            complement(1467..2582)
                     /gene="ald"
                     /locus_tag="VFX34_00310"
     CDS             complement(1467..2582)
                     /gene="ald"
                     /locus_tag="VFX34_00310"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5563370.1"
                     /translation="MRIGIPKEVKNNENRVAMTPAGAFNLKSAGHEVLIETGAGLGSS
                     FTDEDYIEAGAVIVATAEEAWDADMVMKVKEPLASEYGYFREGLILFTYLHLAPELEL
                     TKAMLDNKVVGIAYETVQVNNSLPLLAPMSEVAGRMATQIGAQYLEKINGGKGILLSG
                     VPGVSRGHVTVIGGGQAGTNAAKIAVGMGAKVTVLDLSVDRLRQLDEMFGDSIQTLVS
                     NPFNIAESVKDSDLVIGCVLIPGAKAPKLVSEEMVKSMKPGSVLVDIAIDQGGIFETS
                     DRVTTHDNPTYEKHGVVHYAVANMPGAVPRTSTMALTNVTVPYALQIANKGYKQACLD
                     NAALQKGINTLDGHVTYKAVAEAQGLEYVPAETLLSK"
BASE COUNT          717 a          611 c          582 g          674 t
ORIGIN      
        1 cagttcattc ggcgcgacga taagcgggtt gcaccgtttt gctatgtcaa atgtatcgcc
       61 gacatgatca ttatgtccat gcgtcagcaa aatcacatcc ggagtctgtt ccgatgcttc
      121 caagtccgtc aattcatttc cagtaatgaa cggatcaaac aatagcgtct ttccttccgt
      181 ttcaatcttc acgacagaat gtccgtgata agaaatcttc atttgtaaat ccctccaaat
      241 ctgttcatac atacatactt cgttatttaa ccggaaaacc cttctttttg atatgataat
      301 tatgtagaca taggagggat tggcttggat aaaattaacg aaatcaaaga cttcttgcgg
      361 aaatatgatg tagatgctgc attcattaca actccggaca atgtttttta tgtatccgga
      421 ttccgaagcg agccgcatga aaggctattg ggcgtcatga tcttcaagga tgcggagcca
      481 ttcgtcatct gtccgctgat ggaagtgcct gacttgaaag cgtccgggtg gacatttgaa
      541 gctgtcggcc atcaggatac cgaggacgct tgggaagttg ttgcagctgc agcagcgaaa
      601 agaggaactc ctcttcagac gattgcaatc gaaaaatccc atttgacagt cgagcgtctg
      661 gaaagaatgg aacaactttt tgaaggagcg acattccagc gcctggacga acaactgaat
      721 aacatgcgaa acatcaaaag cgaggatgaa cttgaaaaac tgcggaaagc agcggcattg
      781 gcagatcacg ccatcgaaat cggctgcaaa gagattgccg aagggaaaac cgagcttgag
      841 attctcatgg caattgaatt cgaaatgaag aaaatgggtg cggaaaagat gtctttcgat
      901 acgatggtgc tatccggacc gaaaaccgct tccccgcacg gaacgccagg agaccggaaa
      961 atccagaaag gagatttcat ccttttcgac ctcggcgtcg tatacgaagg gtattgttca
     1021 gatattacaa gaacagtcgc atttggcgaa ccttccgaaa agcagcgtga aatttatgaa
     1081 acagtcaaaa aagccgaaca gacggcaatc gacatgatcc gtccaggcgt gaaagcatgt
     1141 gatttggata aagcttcaag ggatgtcatt acggaggcag gctacggcga gtatttcaca
     1201 caccgccttg gacatgggct tggcatttcc gttcatgaat tcccttcgat tacagggaca
     1261 aatgatatgg aacttgtaga aggaatggtg ttcactgtcg aaccggggat ctatcaccct
     1321 gaaattacgg gtgtgcgaat cgaggatgac gtcgttgtta cgtcagacgg tgtagaagtg
     1381 cttacgaaat tcccgaaaga acttcagatt atcaaaccat gatcaaaaat aaacgctgtc
     1441 cggaacaatg tcggacagcg tttttattat ttgcttaata atgtttctgc tggaacatac
     1501 tccaagcctt gtgcttctgc aacagcttta tacgttacat ggccatctaa tgtgtttatg
     1561 cctttttgca atgcagcgtt atccaagcaa gcttgcttat agcctttgtt tgcaatttgt
     1621 aatgcgtacg gaactgttac gttcgtcaat gccatcgtcg aagtacgcgg aacagctcca
     1681 ggcatgttcg caactgcata gtggacaact ccgtgtttct catacgtagg attatcatga
     1741 gtcgtcacgc ggtctgatgt ttcgaagatg cccccttggt cgattgcgat atcaacaaga
     1801 accgagcctg gcttcatcga ttttaccatc tcttcagaaa ccaatttcgg cgccttggca
     1861 cccgggatca atacacaacc gatcacaagg tcggaatcct tgacagattc agcaatattg
     1921 aatgggttgg aaacaagcgt ctgaatgcta tcgccaaaca tctcatccaa ctgacggaga
     1981 cgatctacag acaaatcaag cacagtcact ttcgctccca tgccaactgc gattttcgcc
     2041 gcgttcgttc cagcctgtcc gccaccgatc acagttacgt gcccgcgcga aacgcctggg
     2101 actcccgaaa gaaggattcc ttttccaccg ttgatctttt caagatattg cgcaccgatt
     2161 tgcgttgcca tacgtcctgc aacttcactc atcggagcaa gcaacggcaa agaattgttc
     2221 acttgcacag tttcgtaggc gatgccgaca actttgttat caagcatagc ttttgtcaat
     2281 tccaattcag gagcaaggtg taaataagtg aataagatga gcccttcacg gaaataaccg
     2341 tactcggatg caagcggttc ctttactttc atgaccatat ccgcatccca tgcttcttcc
     2401 gcagttgcta caattactgc gcctgcttcg atataatcct catcagtaaa gctggaacct
     2461 agaccggcac cagtttcaat cagtacttca tggcctgcag acttcaagtt gaaagcacct
     2521 gcaggtgtca tcgccacccg gttttcgtta ttcttcactt cttttggaat cccaatacgc
     2581 attt
//
LOCUS       DASULI010000016         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_505265, whole
            genome shotgun sequence.
ACCESSION   DASULI010000016 DASULI010000000
VERSION     DASULI010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505265"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFX34_00315"
     CDS             complement(<1..516)
                     /locus_tag="VFX34_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX5563371.1"
                     /translation="MDFKYGGVIMDVINAEQAKVAEAAGAIAVMALERVPSDIRAAGG
                     VARMADPRIVEEVQKAVSIPVMAKVRIGHISEARVLEAMGVDFIDESEVLTPADDEFH
                     LLKSDYKVPFVCGARNLGEAARRLGEGAKMLRTKGEPGTGNIVEAVRHLRQVNAQVNQ
                     IVHMNNDELMVA"
     gene            751..2154
                     /locus_tag="VFX34_00320"
     CDS             751..2154
                     /locus_tag="VFX34_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5563372.1"
                     /translation="MEMLLIELNKESETPLYEQIYQQIRNDITEGKLVVGEKLPSKRQ
                     LGEFLDVSQTTIEIAYGQLAAEGFITSKPRKGYFVQDIGELAYVQPAEAVTIAAKEER
                     KELEIDFSPGKIDTESFPFTQWRKYAKDTIDESSQHLLLLGHPHGDVELRQEIAKYLY
                     HSRGVDCSADQIIVGSGTEQLMPLLIRLLGPQATYAIEDPGYPLTHHVFFHNNREAVP
                     IAVDEEGMDVQALQQSEATVAYVTPSHQFPTGTVLSAARRTALLNWAASSTGNFIIED
                     DYDSEFRYSGRPIPSLQGMDKAGKVIYVSTFSKSLMPSLRIAYMVLPPILLKHYEEAF
                     IHYSSTVPRLDQHTLARFMADGHFARHLNRMRKVYKRKLQLLTDSLRQYAPTISFSGD
                     EAGMHILINVHVDDEEDALSKAALEKGIRVYGLNEYRKAPKSMQPSFLIGFGGLTDDQ
                     IEWAVNQLMDAWNIEKR"
     gene            complement(2371..3714)
                     /locus_tag="VFX34_00325"
     CDS             complement(2371..3714)
                     /locus_tag="VFX34_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5563373.1"
                     /translation="MKREMRKWVALLIIPFVLMMTFGTAPAQAESTLGIHVDGAILID
                     ADSGKILYEENADTPLGIASMSKMMTEYILFEAIKEGKISWDQEYKVTDYTYAISQDR
                     RLSNVPLRKDGTYTIRELYEALAIYSANAATIAIAETIAGTETEFLKLMDAKAKELGL
                     VDYKFVNSTGLNNEYLQGMHPQGTGANDENVMPAKSVARLAFHLMKDYPEVLETTKIN
                     SKIFREGTSDAIKMDNWNFMLPGFVYEYEGVDGLKTGTTDFAGHCFTGTAKRGDKRVI
                     AVVMKAVDANGVGSYKARFDATRALFDYGFSQFSEEELLPAGYQFEKQKTLPVHKGKE
                     KTVAIEVKEPIKAMIKSNEKDLYEPQLVLDESLLKDGALEAPIKKGTVVGHVNLVKKE
                     GEDYGFIDSKDLGMDVVVTNDVEKAGWFSLMMSAIGDFFVGIWESATGFIKGLFK"
     gene            complement(3861..>4066)
                     /locus_tag="VFX34_00330"
     CDS             complement(3861..>4066)
                     /locus_tag="VFX34_00330"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5563374.1"
                     /translation="MPYKGPIADTIHQLVGGIRAGMGYCGTKDLHDLREKAQFIRMTG
                     AGLRESHPHQVHITKEAPNYSIS"
BASE COUNT         1012 a         1076 c          885 g         1093 t
ORIGIN      
        1 ggccaccatc aattcatcat tattcatatg gacgatttga ttcacttgcg cattcacctg
       61 gcgaagatgg cggaccgctt caacgatatt gcccgttccc ggctcacctt tcgtacgcag
      121 catcttcgcc ccttctccga gacgacgtgc tgcttcaccg agattacgcg caccgcagac
      181 gaacggcact ttataatcgc tcttcaataa atgaaactcg tcatctgccg gtgtcaacac
      241 ttcactctcg tcaatgaagt ccacacccat cgcttccaac acacgtgctt ccgaaatatg
      301 tccaatccgt acttttgcca tgaccggaat tgaaactgct ttctgcactt cttcaacgat
      361 gcgaggatcc gccatccggg cgaccccgcc agctgcccgg atgtccgaag ggacacgctc
      421 aagcgccatg actgcaattg ctccagccgc ctccgccact tttgcctgct ctgcgttgat
      481 aacatccata ataactccac catatttaaa atccattttc tcttccccct tttcatttca
      541 ttcagtatac tagagtatga ccatattgaa ataatcagtt tgatgataaa tgaaggggtc
      601 agtttagttc gggcattggg tctaaagtct cgaaatgacg ataactcttg gaatgtgacc
      661 gataactcat tcaaattgac cgatatatcc cgggaaatga ccgataactc tcagggagtg
      721 aacgataact ttttaggcga ggtgcatcaa atggaaatgc ttctcattga actaaataaa
      781 gaaagcgaaa cacctctcta cgagcaaatt taccagcaaa tccgcaatga catcaccgaa
      841 ggtaagctcg ttgtcggcga aaagcttccc tcgaaacggc aacttggtga atttctcgac
      901 gtcagccaga cgacaattga aatcgcttat gggcagctcg cggcggaagg gtttattaca
      961 tccaaaccaa gaaagggcta cttcgtgcaa gatattggag aattggcata cgtacagccg
     1021 gccgaggcag tgacaatcgc cgccaaggag gaacggaaag aattggagat cgatttctcc
     1081 cccggtaaaa tcgacacgga atccttccct ttcacgcaat ggcggaaata cgcgaaggat
     1141 accatcgacg agtcgtcgca acatcttctg ctattgggcc atccacatgg agatgtagaa
     1201 cttcgtcagg aaatcgcgaa gtatttatat cattcgagag gggtcgactg ctccgcggac
     1261 cagatcattg tcgggtccgg tacagaacag ctcatgccat tgctcatccg ccttctcgga
     1321 ccgcaggcaa cgtacgccat cgaggatccg gggtatccac tcacccacca tgtcttcttc
     1381 cataacaatc gggaagccgt gccgattgca gtcgacgagg aagggatgga cgtacaggcg
     1441 ctccaacagt cggaagcaac ggtcgcctat gtaacgcctt cccatcaatt tccgaccggt
     1501 acggtccttt ctgcagcccg caggacagca ttattgaact gggcggcttc cagtaccggc
     1561 aatttcatca tcgaagacga ttacgacagc gaattccgct attcaggacg cccgattcct
     1621 tcattgcaag gaatggacaa ggcaggcaag gtcatctatg taagtacttt ttccaagtcc
     1681 ctaatgccgt cgctgcgaat cgcctatatg gtgctccctc ccattttgtt gaaacactat
     1741 gaggaggcat tcatccatta ttcgtcgacg gtgccgcggc ttgaccagca cacgcttgcc
     1801 cgtttcatgg cggacggcca tttcgcgcgg catttgaatc ggatgcgcaa ggtttataaa
     1861 cgcaaattgc agctccttac ggattcattg cggcaatatg caccgacgat ttcattctca
     1921 ggtgacgaag caggcatgca tattctcatc aatgtccatg tagacgacga agaggatgca
     1981 ttgtcaaagg cggcacttga gaaaggcatc cgtgtttatg gcttgaatga ataccgcaaa
     2041 gccccgaaga gcatgcaacc gtcctttctg atcggattcg ggggcttgac ggacgaccag
     2101 atcgaatggg cggtaaatca gctcatggat gcctggaaca ttgaaaaaag gtgatggatc
     2161 cttgacggat ccatcacctc attttattgt ctagcttcgt gttgcagctg ctcgggtcgc
     2221 ttcgatcttg aagcaaaagg aaaaaagcgc cttttgtcca agccctccag cgaaagccgt
     2281 aacgaagaac ggcttttgcg agtaaaagcg aagcgttacg agcacatctt tgccttgctg
     2341 tcgcagccaa ctgccaccct ccgcttttcg ttatttgaat aaacctttta taaaccctgt
     2401 tgcgctttcc caaattccga cgaagaaatc accgattgcg ctcatcatca acgagaacca
     2461 gccggctttt tcgacatcat ttgtgacaac gacatccatg ccgaggtctt tcgaatcgat
     2521 gaatccgtaa tcctcgccct cttttttaac gaggttcaca tgtccgacaa ccgtcccttt
     2581 tttgattggc gcctcaagtg caccgtcttt caataaagac tcatccagga ctagctgcgg
     2641 ctcataaaga tccttttcat tcgatttgat catcgctttg atcggctctt tcacttcgat
     2701 tgcaactgtt ttctcttttc ctttatggac aggcaatgtc ttttgctttt cgaattgata
     2761 gccggccggc agcagctctt cctcggagaa ctggctgaat ccgtaatcga acaacgcccg
     2821 tgtcgcatca aagcgcgcct tgtaggatcc gactccattt gcatcgacag ctttcatgac
     2881 gacagcgatg acccgcttat cgccccgttt cgccgttcca gtgaagcagt ggccagcgaa
     2941 atcggtcgtt cccgtcttca atccgtcgac gccttcatat tcataaacga aacctggaag
     3001 catgaagttc cagttatcca tcttgatggc atccgatgtc ccttcacgga atattttcga
     3061 attgattttt gtcgtttcca aaacttccgg gtaatctttc atcaagtgga aagcaagccg
     3121 tgcaaccgat ttggcaggca tgacgttctc gtcgttagcc cctgttcctt ggggatgcat
     3181 cccttgcagg tattcattgt tcaaacctgt tgaattgacg aatttataat caaccaaacc
     3241 aagttccttc gctttagcat ccatcaattt caggaattct gtttccgttc ctgcaatcgt
     3301 ttcagcaatc gcgattgttg cagcattcgc tgaataaata gccaatgcct cgtaaagctc
     3361 tctgatcgta tatgttccat ctttacgcag cggcacattg cttagacgcc tatcctgcga
     3421 aatggcatat gtgtaatctg ttactttgta ttcttgatcc caactgatct taccttcttt
     3481 tatcgcttcg aataggatat attcagtcat catcttagac atacttgcaa tgccgagtgg
     3541 tgtatccgca ttctcttcat ataagatctt tccactatcc gcgtcaatta gaatggctcc
     3601 atctacatgg atgccgagtg tcgattctgc ttgtgcgggt gctgtgccga acgtcatcat
     3661 taatacaaat ggaatgatca gcaaagcaac ccattttctc atttcccgtt tcaccaatat
     3721 acctccgtca ttgcaatttt ccactttata tcttatcaca tcatggacgt gtttagggga
     3781 ttcgattgaa aaagcacctt tccgctcata tcaagacgtg cggaaaggtg ctgaagttcc
     3841 atatttacag tttccacttt ttacgagatg gagtagttcg gcgcttcctt cgtaatatga
     3901 acttgatgcg gatggctttc gcgcaaacct gcaccagtca tgcggatgaa ctgagctttt
     3961 tcacgtagat catgaagatc cttcgttccg caatagccca tgcctgcccg gatgccaccg
     4021 acgagctggt gaatcgtatc ggcaattggt cctttatatg gcatac
//
LOCUS       DASULI010000017         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1906190,
            whole genome shotgun sequence.
ACCESSION   DASULI010000017 DASULI010000000
VERSION     DASULI010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906190"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /gene="folK"
                     /locus_tag="VFX34_00335"
     CDS             complement(<1..191)
                     /gene="folK"
                     /locus_tag="VFX34_00335"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5563375.1"
                     /translation="MNTAYISIGSNIGDRLHHLAEAVRALHSHGKIEVTKVSSIYETA
                     PVGYADQADFLNLAVSVETS"
     gene            complement(188..556)
                     /gene="folB"
                     /locus_tag="VFX34_00340"
     CDS             complement(188..556)
                     /gene="folB"
                     /locus_tag="VFX34_00340"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5563376.1"
                     /translation="MDYIHLNEMEFYGYHGALAEENVLGQRFRANVSLAVDLAEAGAT
                     DDLKKTVNYAEVFEVCRSVVEGEPRKLIESVAEEISSLVLERFAGQVRGVRVVLIKPD
                     PPIRGHYASVSVEITRGQFS"
     gene            complement(578..1432)
                     /gene="folP"
                     /locus_tag="VFX34_00345"
     CDS             complement(578..1432)
                     /gene="folP"
                     /locus_tag="VFX34_00345"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5563377.1"
                     /translation="MELSKAKEVYRLGNTEFDFKKETAVMGILNVTPDSFSDGGRYGQ
                     IDAALKRAEEMLREGAKIIDIGGESTRPGHAPVSLDMELERTVPVIEALVRELDCVIS
                     IDTYKADVADAALKAGAKIVNDVWGAKREPRIAEVAAAHGAPIILMHNREKAAYEGPF
                     MDEVIADLEESVSIARKAGVPESDIWLDPGIGFAKDTMQNVLAMQGLRRISDMGYPLL
                     LATSRKRMIGNILDLPVDERMEGTGATVCYGIEHGCHLVRVHDVKEISRMVRMMDVLS
                     GKAEMRFD"
     gene            complement(1437..2285)
                     /gene="pabC"
                     /locus_tag="VFX34_00350"
     CDS             complement(1437..2285)
                     /gene="pabC"
                     /locus_tag="VFX34_00350"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEX5563378.1"
                     /translation="MLCWMNGEFEQAEHLRISPFDHGFLYGAGFFETFRTYEGRVFLF
                     TEHMERLRTALREYRIAMPYSDEEISEAIQGLHDRSGGCDGYFRLNVSAGAHDIGLAP
                     SEYKSPNVILFRKELAEVTRGTEKKAVWLKTERNLPESRIRHKSHNFLNNVRGRLELP
                     SLKELEGIFLTADGHVAEGVTSNVFWVKDGALYTPSIETGILPGLTRGFLLELGAKAG
                     MDVHIGLFGKSDVELADEVFVSNSVQELVPLSAVGEQPMPGASGVYYEKLHRLYVQAI
                     DEMKEG"
     gene            complement(2302..>3485)
                     /locus_tag="VFX34_00355"
     CDS             complement(2302..>3485)
                     /locus_tag="VFX34_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX5563379.1"
                     /translation="LDLVSAFVESMQMDSVPELPDFQGGAIGFISYDYARTYESIPEI
                     AVDDLETPDIFFYLFDRWAVLDLEAETAYFMTLPGRGLDVLEMEKEWMAAAEVGIGKR
                     SIVHASSSIDYTRPENVEVSVTGPQFEQMVRDVQRYIADGDVNQVNLTVRQSKKLSVD
                     PLSMYEALRSFNPSPYMALIGAKEFAVVSGSPELLLKKRGTELSTRPIGGTRPRGASE
                     TEDAAFEQDLLSNEKEKGEHIMLVEQELEDFGQVCVPETVETDEFMVVERYSHVMHLV
                     SNVRGTASPELSNAAIIRGVFPGGSITGSPKLRTMEIIEELEPTRRGLYTGSIGWIGF
                     NGDMDLNIVIRTAYIKDGIAHIQAGAGLVADSDPRAEYEESLNKARALWQAKEMAEAA
                     E"
BASE COUNT          811 a         1074 c          740 g          860 t
ORIGIN      
        1 gaagtctcaa cagagacggc caaattcagg aagtccgcct ggtccgcata cccgacaggc
       61 gctgtttcat agatggaaga caccttcgtc acttcaatct ttccgtggga atgaagtgcc
      121 cgtaccgcct ctgcaagatg atgcagcctg tccccgatat tggatccaat cgaaatgtat
      181 gcggtattca tgaaaattgc cctctcgtga tttcaacaga taccgaggca taatgccccc
      241 ggatcggcgg atcgggtttt attaagacga cccggacgcc gcggacctgc cccgcgaacc
      301 tttctaaaac tagactggag atttcctcgg ctacggattc aatcagttta cgtggctccc
      361 cttctactac tgaacggcaa acttcgaaca cttctgcata attgaccgtc ttcttcaaat
      421 catccgttgc gcccgcttca gctaaatcga ccgccagcga tacattcgcc cgaaaacgct
      481 ggccgaggac attctcttcc gccaatgcac cgtggtagcc gtaaaactcc atttcattca
      541 aatgaatata atccattttc aaaccctcca taagcaatca gtcgaaacgc atttccgcct
      601 tgcctgatag tacatccatc atcctcacca tgcgggatat ctctttcacg tcgtgtaccc
      661 gcaccaaatg gcagccgtgc tcgataccat agcaaaccgt cgcgccggtc ccttccatcc
      721 gctcgtcgac cggcagatcc aaaatattgc cgatcatccg tttccgggat gtggcaagca
      781 atagcggata ccccatatcc gaaatgcggc gaagcccttg catcgctaag acattctgca
      841 tcgtatcttt cgcaaagccg atgcccggat cgagccaaat atcgctttcc ggcactcccg
      901 ctttgcgtgc aatcgacacg ctttcctcca agtccgcgat cacttcatcc atgaacggcc
      961 cttcgtatgc cgccttttcc cgattatgca tcagaatgat cggcgctccg tgagcagccg
     1021 ccacttccgc aatccttggc tcgcgtttcg cgccccatac gtcattgaca attttagcgc
     1081 ccgctttcaa tgcagcatcc gccacgtccg ccttgtatgt atcgattgaa atgacacagt
     1141 ccagttcccg aactagcgcc tcgatcacgg gaaccgtacg ctcaagttcc atatccagcg
     1201 agacaggggc gtgcccgggt cgggtcgatt ccccgccgat atcgatgatt ttcgcacctt
     1261 cacgaagcat ttcttccgca cgcttcaatg cggcatcgat ttgtccgtat ctgcctccgt
     1321 ccgagaagga gtccggcgtg acattcaaaa tgcccatcac agccgtctcc tttttaaagt
     1381 cgaattcggt attccctagg cggtatacct ctttagcttt cgataattcc atcaatttac
     1441 ccttctttca tctcatcaat cgcctgaacg tataatcgat gcaatttttc ataataaaca
     1501 cccgaagctc cgggcatcgg ctgctcgcct accgcagaga gggggacgag ctcctggact
     1561 gagttcgaaa cgaatacttc gtcagctagc tcgacgtcac ttttcccaaa cagcccgata
     1621 tgtacatcca ttccagcctt cgccccaagc tccaataaaa agccgcgtgt aagaccaggc
     1681 agaatccctg tttctattga cggcgtgtat aacgctccgt ctttcaccca gaacacattg
     1741 gacgtcacac cttcagcgac atgcccatcc gccgtgagaa aaatcccttc cagctccttc
     1801 aaagacggca gctccaaacg cccgcgcaca ttgttcaaga agttatgaga cttgtggcgg
     1861 atccggctct ctggcagatt cctttccgtc ttaagccata ctgccttctt ttccgtaccc
     1921 cgcgttactt ccgcaagctc tttccggaac aggatgacgt tcggcgactt gtattcggaa
     1981 ggagccaagc cgatatcatg cgcaccggct gacacattca gccggaaata cccgtcacat
     2041 ccacccgaac ggtcatgcaa tccttgaatc gcttccgaaa tctcttcatc cgagtaaggc
     2101 atcgcaatcc ggtattcgcg cagtgctgta cgaagacgtt ccatatgctc tgtgaataaa
     2161 aacacgcgtc cctcatacgt cctgaacgtc tcgaaaaaac ccgcgccata tagaaaccca
     2221 tgatcgaacg gggaaatcct caagtgttcc gcctgttcaa attcaccgtt catccaacat
     2281 agcatggagg tgattctcct ttcattcagc cgcttcggcc atttccttcg cctgccagag
     2341 cgccctcgcc ttattgagcg actcctcgta ttccgctctc ggatcggagt cagcgacaag
     2401 acctgcacct gcctgaatat gggctattcc atcttttata tacgctgtcc ggatgacgat
     2461 attcaaatcc atgtcgccgt taaagccgat ccagccaatc gaccccgtat ataagcctct
     2521 ccgagtcggt tctaactcct cgatgatttc catcgtgcgc agtttcggag agcctgtgat
     2581 cgaacctccc gggaacacgc ccctgatgat ggcagcattc gataattccg gagatgccgt
     2641 ccctcttaca ttcgaaacaa gatgcatgac gtgcgaatac cgctcaacga ccatgaattc
     2701 atccgtttcc accgtttccg gcacgcagac ttgtccgaag tcctccaatt cctgttcaac
     2761 aagcataata tgctcgcctt tctccttttc attggagagc aagtcctgtt caaacgctgc
     2821 atcttcggtt tccgaagccc ctcgcggcct cgtgcctccg attggacgcg tgctcaactc
     2881 ggttccgcgc ttcttcagca gcaattccgg cgagccggag acgactgcaa attccttcgc
     2941 tccgattaac gccatataag gtgatggatt gaaggaccgc aatgcttcgt acatagatag
     3001 cggatctact gatagctttt tagattgacg gacggtcaag ttgacttgat tgacgtcgcc
     3061 gtccgctata tatcgctgga cgtcgcgcac catctgttca aattgcggtc ctgtcactga
     3121 gacttccaca ttttccggtc ttgtataatc gattgaactg ctagcgtgta ctatgctgcg
     3181 cttcccgatc ccgacttccg ctgccgccat ccattccttt tccatttcca acacatcgag
     3241 cccccgtccg ggcagcgtca tgaaataggc tgtttccgct tcgagatcga ggacagccca
     3301 acggtcaaac aagtagaaga agatatccgg cgtctcaaga tcatccaccg caatttcagg
     3361 gatcgactca tacgttctag cataatcata gctgatgaaa ccgatggcgc ctccttgaaa
     3421 atccggcaac tcgggcacac tgtccatttg catcgattcg acgaacgcag ataccagatc
     3481 aagcg
//
LOCUS       DASULI010000018         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1108273,
            whole genome shotgun sequence.
ACCESSION   DASULI010000018 DASULI010000000
VERSION     DASULI010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108273"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="prsW"
                     /locus_tag="VFX34_00360"
     CDS             <1..627
                     /gene="prsW"
                     /locus_tag="VFX34_00360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamic-type intramembrane protease PrsW"
                     /protein_id="HEX5563380.1"
                     /translation="PSRTLFHTFIYGAMMTFPIMFIQHVFEEENIIPNEFFRNAIFTS
                     WIEEFFKWLILLVAIFKHVEFEDAYDGILYGASVSLGFATVENILYLFIFGLDTAFLR
                     ALLPVSSHALFGVVMGYYLGRAKFTTFSNRKGMLFFAFFAPFILHLIYNGILGVSDLS
                     LYLIIPFMLYLWWFGLTRVKFAHKFAMQQFRSRVHDKIHYSSNENDSI"
     gene            746..1516
                     /gene="sleB"
                     /locus_tag="VFX34_00365"
     CDS             746..1516
                     /gene="sleB"
                     /locus_tag="VFX34_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HEX5563381.1"
                     /translation="MRILAVALIIGTAYATSPIEIDAFSSRDIARGATGDDVIELQAR
                     LQYIGFYNGKIDGKFGYGTYWALRNFQEKYGLPIDGIAGSQTKKKLTAASKYDEKFVK
                     DNLKKGNQFTYYGGTPLAAQVKKGGGGKENMHLPSKYSEQDLKLMANAVYGEARGEPY
                     EGQVAVAAVILNRVEHPDFPDTISGVIFQPLAFTAVADGQIWLEPNQRAKEAVLDALN
                     GWDPSENAIYYFNPITATSKWIWSRPQIKKIGLHIFCR"
     gene            1568..>2289
                     /locus_tag="VFX34_00370"
     CDS             1568..>2289
                     /locus_tag="VFX34_00370"
                     /inference="COORDINATES: protein motif:HMM:NF044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore gernimation protein"
                     /protein_id="HEX5563382.1"
                     /translation="MLTMKKMIFVLAYALVVVSVFAFTKATENNKLAILLSNSYAQQM
                     ADASDKMDELDSAVKQTLLFNHSEGSKKAREDIWRLSSDIKRSVASLPLDSAFSTSWI
                     NYLGRLGNYAKEADRLTDPGEYHKVMSQASKNLGVMHEDWMLATSGLMDGQYSMNEWT
                     NRLNTANPNFDWANMGTSVKQYTEADFPLTASESDSMKKKDLKNIDEAKVTQAEAVDR
                     FKTLFPQVSNDVVGVEMSKPGSP"
BASE COUNT          667 a          448 c          546 g          628 t
ORIGIN      
        1 ccttcgcgga ccctattcca tacattcata tatggggcaa tgatgacctt tccgatcatg
       61 ttcattcagc acgtttttga agaggagaac atcatcccga atgaattctt tcgcaatgca
      121 atattcacaa gttggatcga agagtttttc aaatggctca tattgttggt cgcaatcttc
      181 aaacacgtgg aattcgagga tgcttacgat ggcatcttat atggggccag tgtgtcactc
      241 ggttttgcga cagtggaaaa catcctttat ctattcattt tcggactgga cactgcgttc
      301 ctgagagcac ttctgcctgt atcgagccac gctctattcg gcgtcgtgat gggctattat
      361 ttaggaagag cgaaatttac aactttctca aatcgaaagg gaatgttgtt cttcgccttt
      421 tttgccccat tcatcctcca tctcatttac aatggaatct taggggtgag cgacctctca
      481 ttatatttaa tcattccatt catgctttac ctttggtggt tcggattgac acgggtgaaa
      541 tttgcccata aatttgcaat gcagcaattc aggagtagag ttcatgataa aatacattac
      601 tcttcaaatg agaatgatag catataacat tcaagagaag atctggccct taagccagat
      661 ctttttttta tgcaatcaag ttaataaaaa gaatttgttt ggacaaactg atagtaagga
      721 gggtatgaat ttgaagaaaa tattgatacg gatattggct gtggcattaa taattggtac
      781 ggcttatgca acttccccta ttgaaatcga tgcgttcagc tcccgcgata ttgcaagagg
      841 ggcgacgggg gatgatgtaa tcgaacttca agctagactt caatacatcg gtttctataa
      901 tgggaaaatt gatggaaagt tcgggtatgg aacttattgg gccttgcgga atttccaaga
      961 gaagtacggg ttgccgattg atggaattgc cggctcgcaa acgaaaaaga agttgacagc
     1021 agcatcgaag tacgatgaaa aatttgttaa ggataatttg aagaagggaa atcagttcac
     1081 ctattatgga ggcactccat tagcagccca ggtgaaaaaa ggcggcggcg gaaaagagaa
     1141 tatgcatttg ccgtcaaaat actccgagca ggatttgaaa ctcatggcaa atgccgttta
     1201 tggggaagca aggggggaac cttatgaggg tcaagtggca gtcgcagctg taatcttgaa
     1261 ccgggtggag catcccgatt tccccgatac gatcagcggc gtcattttcc aacctttggc
     1321 gttcaccgct gtagctgacg gacagatatg gcttgagccg aatcaacgtg cgaaggaagc
     1381 cgttcttgat gcattgaatg gctgggatcc ttccgaaaat gccatttatt atttcaatcc
     1441 gataaccgct acaagtaaat ggatttggtc gagaccgcaa attaagaaga tcggcttgca
     1501 tatcttttgc cgttaatggc tacgtgaaag gtggattgaa taatagctct ttatggtagg
     1561 gctatttatg ttgactatga aaaaaatgat ctttgtactt gcctacgcgt tagtcgtcgt
     1621 ttcagtcttc gcgtttacaa aggcaactga aaataacaaa cttgcgattt tattaagcaa
     1681 ctcatatgcc caacaaatgg cggacgcctc agacaaaatg gatgagctag attctgccgt
     1741 taaacaaacg cttctcttca atcattcaga aggctcgaaa aaagcgcggg aagatatttg
     1801 gaggctttca tcggatatta agcgctctgt cgcttcattg ccgttggact cggctttttc
     1861 gacttcgtgg ataaactatt tggggagact cggcaattac gctaaagagg ccgatcgatt
     1921 aaccgacccc ggggaatatc ataaagtgat gtcacaagct tcgaaaaacc taggcgtcat
     1981 gcatgaagat tggatgcttg caacatctgg acttatggac ggacagtatt cgatgaatga
     2041 atggacgaat agactcaata cggcgaatcc gaattttgac tgggccaata tgggaacgtc
     2101 cgtcaagcaa tacacagaag cagactttcc tttgacagca agtgagtcag attcaatgaa
     2161 aaagaaggac cttaagaaca tagatgaagc gaaagtgacg caagcggaag cggttgatcg
     2221 gttcaagaca ttattccctc aagtctcgaa tgacgtagtc ggtgtggaga tgagcaagcc
     2281 aggatctcc
//
LOCUS       DASULI010000019         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1419586,
            whole genome shotgun sequence.
ACCESSION   DASULI010000019 DASULI010000000
VERSION     DASULI010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419586"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFX34_00375"
     CDS             <1..449
                     /locus_tag="VFX34_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEX5563383.1"
                     /translation="EGTYLADFKKHQLVEVLSTFIMQQPKSAMDVTDTRIIHEKAAIQ
                     TICENAQKRKLPVWEGLLMKLENEGAVFREDIIREMIVATDNRLSLKIWFILKRYSKV
                     PFEQMTEGTENDIIRALLKHLMDGNVMEAIYAYNQWIEHIEGENVE"
     gene            446..1300
                     /gene="accD"
                     /locus_tag="VFX34_00380"
     CDS             446..1300
                     /gene="accD"
                     /locus_tag="VFX34_00380"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX5563384.1"
                     /translation="MIREFFTKKKQAITIPSADAKNDVPEGIMTKCPDCKQIILTKDL
                     MKTAKVCPKCDHHFKMTAWERVECLFDENTFTSMDDHLKSENPLNFPSYTEKIALDSK
                     KTGLNEAVLTGIGKIGGNEIAIAIMDSHFRMGSMGSVVGEKITRAVEAATERGIPVII
                     FSASGGARMQEGVLSLMQMAKTSVALERHANKGLLFISVMTYPTTGGVSASFASVGDI
                     NLAEPRALIGFAGRRVIEQTVREKLPDDFQTAEFLLEHGQVDAVIDRRELTEALAKLI
                     GLHVREGK"
     gene            1302..2255
                     /locus_tag="VFX34_00385"
     CDS             1302..2255
                     /locus_tag="VFX34_00385"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5563385.1"
                     /translation="MSKTLAFEEPIVKLREKINELEEFTSTNEVDLSDEIGKLKTRLS
                     NLETEIYSNMEPWDRVQVARHPERPTTRDYVAELFEDFMELHGDRSFGDDAAIIGGIG
                     SFESMPVTVIGHQRGKDTKENVRRNFGMPHPEGYRKALRLMKQAEKFGRPIICFIDTK
                     GAYPGKAAEERGQSEAIARNLIEMAGLKVPVISIVIGEGGSGGALALGVANHIHMLEH
                     STYSVISPEGAASILWKDASLSKQAAEAMKITAPHLKEMGIIDEIIPEVLGGAHRDPQ
                     AQAVEMRKVIRTSLDSLCKMDANAIIDDRYNKFRNIGVFSE"
     gene            2458..3420
                     /gene="pfkA"
                     /locus_tag="VFX34_00390"
     CDS             2458..3420
                     /gene="pfkA"
                     /locus_tag="VFX34_00390"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX5563386.1"
                     /translation="MKKIGVLTSGGDAPGMNAAVRAVVRKAIYEGVEVAGVFNGYQGL
                     IQGKIELLQLGSVGDIIQRGGTMLRSARSPEFMTEEGRKEAIRQLKAHHIDGLVVIGG
                     DGSFRGAFELVKMGIPCVCVPATIDNDINGTEFTIGFDSALNTVIDAIDKIRDTATSH
                     ERTFIIEVMGRDAGDLALWAGLAGGAETILIPEEGYVMDEIINRLQSGTGRGKKHSII
                     VVAEGVMSGSALADLLKEKAGIETRVSVLGHIQRGGSPSARDRVIASQYGARAVEVLG
                     EGRGGVAIGMQNHKVVDYDLEVVFAPKDHTLDMGLYKLSKELSI"
     gene            3470..>3581
                     /locus_tag="VFX34_00395"
     CDS             3470..>3581
                     /locus_tag="VFX34_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002122980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5563387.1"
                     /translation="MRKTKIVCTIGPASESPELLEQLIEAGMNVARLNFSH"
BASE COUNT         1024 a          719 c          983 g          855 t
ORIGIN      
        1 gagaagggac ataccttgcc gatttcaaaa agcatcagct cgttgaagtg ctttcgacgt
       61 ttatcatgca acagccaaaa tcggcgatgg atgtgacaga tacccgcatc atccatgaaa
      121 aggcggcaat ccaaacaatc tgcgaaaatg cacaaaaacg caaactgcct gtctgggaag
      181 ggctgcttat gaagctcgag aatgagggcg cggttttccg tgaggatatt attcgggaaa
      241 tgatcgttgc gacagacaac cgattatcat taaaaatctg gtttatactg aaacgatata
      301 gcaaggttcc attcgagcag atgacggaag gaacagaaaa tgatattata cgtgctttac
      361 tcaagcattt gatggacgga aacgtcatgg aagcgatata tgcttataac cagtggattg
      421 aacatatcga gggggaaaac gtagaatgat ccgtgaattt tttacaaaaa agaaacaggc
      481 gataacgata ccttcggccg atgctaaaaa tgatgtgccg gaagggatca tgacgaaatg
      541 tccggattgc aagcagatta tcctgacgaa agatcttatg aaaacagcaa aagtttgtcc
      601 gaaatgcgat catcatttta aaatgacggc atgggagcgt gtcgaatgtc tttttgacga
      661 aaatacattt acatcaatgg acgaccattt aaaatcggaa aaccctttga acttcccatc
      721 ctacacagag aaaattgcgt tggattctaa gaagacagga ttgaatgaag cggttcttac
      781 cggaatcgga aaaatcgggg gaaatgaaat tgcaatcgca atcatggatt cccatttccg
      841 gatgggctca atggggtctg ttgtcggcga gaaaatcacc cgtgcggttg aggccgccac
      901 agaaaggggc attcctgtca tcatcttttc cgcgagcggc ggagcacgta tgcaggaagg
      961 tgttttgtcg ttaatgcaga tggcgaaaac aagtgttgca ttggaacgtc atgccaacaa
     1021 aggactcttg ttcatttccg tcatgaccta tccgacaacg ggcggtgtat ccgcgagctt
     1081 tgcgtccgtc ggcgatataa atttagccga accgcgggca ttgatcggat ttgcaggaag
     1141 acgggtgatc gagcagactg taagggaaaa gctgccggac gatttccaga cagccgaatt
     1201 cctattggaa cacggacaag tcgatgctgt catcgatcgc cgtgagttaa ctgaagccct
     1261 tgcaaaactg atcgggctgc atgtacggga gggtaaataa gatgagcaag acgttggcat
     1321 ttgaagaacc gattgtgaaa cttcgggaaa agattaatga acttgaagag ttcacttcaa
     1381 cgaacgaagt cgatctttcc gatgagatag gtaaattgaa aacaagatta agcaatttgg
     1441 aaacggaaat ctatagcaat atggaaccgt gggaccgcgt acaagtagcg agacaccctg
     1501 aacgtccgac gacgagggat tatgttgcgg aactatttga agatttcatg gagctgcatg
     1561 gagatcgctc atttggtgac gatgcagcaa tcatcggcgg aatcggctcc tttgaatcga
     1621 tgcctgtgac ggttatcggt catcagagag ggaaagacac gaaggaaaac gtgagacgca
     1681 atttcgggat gccgcatccg gaagggtatc gaaaagcatt gcggttaatg aaacaggcag
     1741 aaaaattcgg ccgtccgatc atctgcttca tcgatacgaa aggcgcttat ccagggaaag
     1801 ctgctgaaga acgcggtcaa agcgaagcga ttgcccgcaa tctaattgaa atggcgggat
     1861 tgaaagtgcc ggttatttca atcgtcatcg gggaaggcgg aagtggaggc gctctcgctt
     1921 taggcgttgc caatcacatc catatgttgg agcactcgac gtattccgtc atttcacctg
     1981 aaggagcggc ttcgattctt tggaaagatg cgagcctttc gaaacaggca gcagaggcga
     2041 tgaagattac agcacctcat ttgaaggaaa tgggaattat cgatgaaatc atccccgaag
     2101 tgcttggcgg tgcgcatcgg gatccgcagg ctcaagctgt agaaatgagg aaagtgataa
     2161 ggacttcatt ggactcactt tgtaaaatgg atgcaaatgc aattatagat gaccgatata
     2221 ataagttccg taatatcggc gtattttcag aataaggtgc gggctttccc cgcatctttt
     2281 cgttgcgtct agactcctgg cgccagaggt tcggggtcat aagtcaaagt tgctctgtgg
     2341 caaacggcgc cacgccgcat cttcgactta tgcccgtcac ctctaggcag gcgccctgcg
     2401 cttttgttcg gttatacggt attatacata tattgaatta cataaggggt ggtccatttg
     2461 aagaaaattg gcgttttgac aagcggggga gacgcgcctg gcatgaatgc agctgtccgt
     2521 gcggtcgtac ggaaggcgat ttatgaaggg gttgaagttg caggtgtttt caatggctat
     2581 caaggactca tccaaggaaa aatcgagctg ctgcagcttg gatctgtcgg tgacatcatc
     2641 caacgcggcg ggacaatgct tagatccgcc cgttctcctg aattcatgac tgaagaaggc
     2701 cgtaaagaag cgattcgcca gttgaaggcg catcatatcg atgggcttgt cgtcatcggg
     2761 ggagatgggt cattcagggg tgcgttcgaa ttggtgaaaa tggggattcc ttgtgtttgt
     2821 gttcccgcta caatcgataa tgacattaat gggaccgaat tcaccattgg tttcgattcc
     2881 gcattaaata ccgtcattga tgccatcgat aagatcagag ataccgccac atcgcatgaa
     2941 cgaactttca ttatcgaagt gatgggccgc gatgcagggg atttggcgct ttgggctggc
     3001 ctagcgggag gagcggagac gattctcata cctgaagagg gctatgttat ggatgaaatc
     3061 ataaacagat tacaaagcgg aacaggccgt ggcaagaaac atagtatcat tgtcgtagcg
     3121 gaaggcgtca tgtcaggatc agctttggct gatctgttga aggagaaagc gggaatcgag
     3181 acccgtgttt ccgtcctggg gcatatccag cgcgggggtt ctccatctgc ccgcgaccgg
     3241 gtcattgcga gccaatatgg cgcaagggcg gttgaagtat tgggagaagg acgtggcgga
     3301 gtcgctattg gtatgcaaaa ccataaggtg gtagactatg atttagaagt ggtatttgca
     3361 ccaaaagacc atactttgga tatgggtctg tacaagttat ccaaagaatt gtcgatctga
     3421 tctaacgaat acatttgatg agaacaatac gaaggggcgt tttgtgatga tgagaaagac
     3481 aaaaattgtg tgcacaatcg gaccggccag cgaatcacct gagctgcttg aacagttgat
     3541 agaggcaggc atgaatgtag cacggcttaa cttttcacat g
//
LOCUS       DASULI010000020         4713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_601628, whole
            genome shotgun sequence.
ACCESSION   DASULI010000020 DASULI010000000
VERSION     DASULI010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601628"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFX34_00400"
     CDS             complement(<1..568)
                     /locus_tag="VFX34_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563388.1"
                     /translation="MKSNRFMKWVIIGVVALMAGIGNLFGTGKMASAEQTVLVEEDFD
                     QLEGAMPEGWNVIQGQASVEDGALKLTSPSSASPSRVLVPLPEKNGNIVFEADMTFVS
                     AVEDTRWASMMFRVQSGDYPYYQFAIRRGTNALNGAEFAERNAQNKWVVPEATFFTEK
                     FEFNKPYRIKIIASNNRVQQFINNKLVIN"
     gene            963..1994
                     /locus_tag="VFX34_00405"
     CDS             963..1994
                     /locus_tag="VFX34_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5563389.1"
                     /translation="MKRFGGLAASVLLATGLLAGCAGGASEGGGDGKGGRITLYSPET
                     PDFTKELAQKYEELNDEKVDVHYAGTNILVNQMMAEKDNPKADVWYGGGGILPFEAAV
                     ERGILASYIPDFAADWDVVENGIKMKHEDGKYVGVELFVLGFSYNTDLVSEEEAPKTW
                     ADLLDPKWEGKIQFPNPAASGTATLMVMDQMMQQGEDKAWEYFEKLVKQANSIPDSGS
                     APTKAVAMGEAHIGVGFDFMAYEQKAKGETVDFVVPDKTPVLVNPVSLIEGGPNAEGG
                     KKFVDFLLSKEAQQIMADWYHIPINPDVESKTPLTLEKVKEHAVDLDIDWVNENYDRV
                     RNEWKDKFQ"
     gene            2011..3141
                     /locus_tag="VFX34_00410"
     CDS             2011..3141
                     /locus_tag="VFX34_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017797904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563390.1"
                     /translation="MGSVKIENMTKKFGDFTALHDINLDIEEGEFFALLGPSGCGKTT
                     TMRCIAGFENPSSGSIYIGDREVEKIPANRRNCGMVFQSYALFPHMNVFENVAYSLNI
                     KQLNASNPAKQLAIYARLLTKRFGKYPDDLRNKVMEILKYVELENHADRLTSELSGGQ
                     QQRVALARALVMEPAVLLMDEPLSNLDQKLRHSMRNTIRTIQQDLGITTIFVTHDQEE
                     AMSMADRVAVMDKGHIVQIGTPTDLYSNPQTPFIANFVGTSNILKGDVQGEENGLTIV
                     KGEGFLLRSSNKAKQPEVDVIIRPEHVKVEAADSVIDHAKTNVIEGTVIMSTYLGSIV
                     RYDVQVGSYQLIVDTTYSSGANIFEEGKKVKLTVEHERVLLI"
     gene            3138..>4713
                     /locus_tag="VFX34_00415"
     CDS             3138..>4713
                     /locus_tag="VFX34_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011067116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5563391.1"
                     /translation="MNRFKNSFSGLTVVKVLIYAFFGLFMLLPLLSVFVVSFTGEPTN
                     LLGSLTDPGIFNSTVEKFKNMSLDNYKRIFSGKGYFDALKNSLALAFTVTIIIILVCI
                     PLAYGIARTKMPFKKTISALCMIPLIVPTFISAYAFIIMFGRAGWVTQIYNALGGEGM
                     LLNPYSMAGVVAVQVFFFFPYALWPLVAAFKVSDISLEEASQNLGAKSWFTFIFVTFP
                     LALPGVISSALLIFTVSFSDFGSPIVLAPKELNLLVVEAYREIAGFFNWGGAAILTVV
                     MVVVAAFFFYLQHLFTKGRNYGSVSGKPKQQKLIENKLLTRVLSGYSLFVVLIPLLAM
                     LSVALQSVATTWGKDLLPSGYTLNHYKTIFSSSMGNIQNSIMLATGALVLSVIVATFV
                     SYFVVRQNSAKLDFIASIPLVVPGIAFGIALIQTFNTAPLQLTGTAVLLIIAYTIRRL
                     PYMVRSTMGSMRAIKQDIEEAAVNLGATPLTAAITIVGPLMLPGIAAGSVLVFITVIK
                     EASVSILLAPPEWAPMS"
BASE COUNT         1165 a         1038 c         1277 g         1233 t
ORIGIN      
        1 tattgatgac aagcttgtta ttgatgaatt gctggacgcg gttattgctt gcgatgatct
       61 tgatgcggta cggcttgttg aactcgaact tttccgtgaa gaacgtcgct tcgggcacga
      121 cccatttgtt ctgggcgttt cgctctgcaa actcggcgcc gttcagcgcg ttcgtgcctc
      181 tacggatcgc gaattgatag tatggataat ctcccgattg cacgcggaac atcatcgatg
      241 cccagcgagt gtcttcaacc gcggaaacga atgtcatatc cgcttcgaat acgatgtttc
      301 cgttcttttc agggagcggt acaagcaccc tcgacggtga agctgacgaa ggcgaagtca
      361 acttcaatgc tccgtcttca acggaggctt gtccttgaat gacgttccat ccttccggca
      421 tcgcgccttc cagctggtca aaatcctctt cgacgagcac ggtctgttcc gcgcttgcca
      481 tcttgcccgt gccgaataag ttgccgatgc ccgccatcag cgcgacaaca ccaatgatga
      541 cccatttcat gaatctgttg cttttcatcc caattcctcc tttcgaatta gattgctaat
      601 ctagcaacct aggtagcaat cggttagaag tatacatgat tcgtaattat ttgaaagtta
      661 aaatatccat agctggccaa aaacaattct tttctactgg aaatgcgaca taatacgctt
      721 ctaacgaact atttttcctc ctagggggac agtcgcaaaa ctggattgat gaatgaaatg
      781 tgcaaatagt gtctataaaa acgcaatata ctattgcaat tgtatgaatc tactgtatac
      841 tatgggtagt tgaatatagg cagagacgat gagatgaacg cctaaaaatg taagcccttt
      901 ctaggggttc atttggcgtt cttctctttt ttcttgccta aaaaaatatt agggggattt
      961 tcatgaaaag atttggtgga ttagcagcta gcgtcctgtt ggcaacggga ttgcttgccg
     1021 gttgtgctgg gggagcatcg gaaggcggag gagatggcaa agggggcagg atcactcttt
     1081 attcacctga aactccggac ttcacgaagg agttggctca gaagtatgag gagctgaatg
     1141 acgagaaagt tgacgtccat tatgccggga cgaatatcct tgtcaatcag atgatggctg
     1201 agaaggacaa tccgaaagcg gacgtctggt acggcggggg gggcatcctt ccgtttgagg
     1261 ctgcagtgga acgcggaatc ctcgcttcct acatcccgga tttcgcagcg gattgggatg
     1321 tcgtcgagaa cggcattaaa atgaagcacg aagacggtaa atatgtcggt gttgaactgt
     1381 tcgtactagg attttcttat aatacggact tggtgtctga agaagaggcg ccgaagacgt
     1441 gggcagattt gcttgacccg aaatgggaag ggaagatcca attcccgaac cctgcagcat
     1501 ccggtacagc gactctgatg gttatggacc agatgatgca gcaaggggaa gacaaggctt
     1561 gggagtattt cgaaaaactt gtgaagcaag caaactcgat tcctgactcg ggttccgctc
     1621 ctacgaaagc ggtagcgatg ggtgaagcgc atatcggcgt cggcttcgac ttcatggcgt
     1681 atgagcaaaa ggcgaaaggc gagactgtgg acttcgttgt tccggataag acaccggtac
     1741 tcgtcaaccc tgtttcattg attgaaggcg gcccgaatgc tgaaggcggc aagaaattcg
     1801 ttgacttcct tctatccaag gaagcccaac agatcatggc ggattggtac catatcccga
     1861 tcaacccgga tgtagagtcg aaaacgccgt tgacgcttga aaaggtgaaa gaacacgctg
     1921 tcgatcttga tattgattgg gtgaatgaaa actacgaccg cgttcgtaac gaatggaaag
     1981 acaaatttca ataattaagg gtgaatatgt atggggtcag taaaaattga aaacatgaca
     2041 aagaagtttg gggactttac tgctcttcac gacattaacc ttgatatcga ggagggcgaa
     2101 tttttcgctc ttctcggtcc ttcgggatgt gggaaaacga caacgatgcg gtgtatcgcc
     2161 ggatttgaaa atccttcgtc gggatccatc tacataggag atcgtgaagt ggaaaagatt
     2221 ccggcaaaca gacggaattg cgggatggtc tttcagagct atgctttatt tccacatatg
     2281 aatgtgtttg aaaacgttgc ctacagcttg aacatcaagc agctgaatgc aagcaacccg
     2341 gctaaacagc tggcgatcta tgcgcgtctg ttgacgaagc gtttcggaaa atatccggac
     2401 gatctgagaa ataaagtgat ggaaatattg aagtacgttg agttggaaaa ccatgcggat
     2461 cggctgacga gtgaattatc gggcggacag cagcagcggg tggctttggc aagggcattg
     2521 gtcatggagc ccgcggtttt gctcatggat gagccgttgt ccaacttgga ccagaaattg
     2581 cggcattcga tgcggaacac gatccggacg attcaacagg atctgggcat tacgacgatc
     2641 ttcgtcacgc acgaccagga agaggcgatg agcatggcgg accgtgtcgc ggtcatggat
     2701 aaaggccata tcgtccaaat cgggacgccg acagatctgt acagtaatcc acagacaccg
     2761 ttcatcgcga atttcgtcgg gacgtcgaac atcctgaaag gcgatgtcca aggcgaggag
     2821 aatggactca ctatcgtcaa aggggaaggg tttctacttc gttcgtcaaa caaggcgaag
     2881 caaccggaag tggatgtcat catccgtccg gagcacgtaa aagtggaggc ggcggacagt
     2941 gtgatcgatc atgcgaagac gaatgtgatc gaagggacgg tcatcatgtc cacgtatcta
     3001 gggtcgatcg tgcgttacga cgtacaagtc ggctcgtatc aattgattgt cgatacgacg
     3061 tattcctcag gcgccaatat ttttgaggaa gggaagaaag tcaagttaac tgtggaacac
     3121 gagagggtgc tgttaatatg aaccgtttta aaaacagctt cagtggatta acggtcgtaa
     3181 aagtactcat ttatgcattc tttggcctgt tcatgttgct gccgctcctc tcggtattcg
     3241 ttgtcagctt caccggtgaa ccgacgaatc tgttgggttc cttgacggat ccggggattt
     3301 tcaattccac ggttgaaaag ttcaagaaca tgtcgttgga caattataag aggatcttct
     3361 cgggcaaagg ttattttgat gcattgaaaa acagccttgc attggcgttt accgtcacca
     3421 tcatcattat cctcgtttgt attccccttg cgtatgggat tgcaaggacg aaaatgcctt
     3481 tcaagaagac gatttccgca ttatgcatga ttccattgat tgtcccgacg ttcatttctg
     3541 catatgcgtt tatcatcatg ttcggtcggg ctggatgggt gacgcaaatc tataatgcgc
     3601 tgggcgggga aggcatgctg ttgaacccct attcgatggc cggggtcgtt gccgtgcaag
     3661 tgttcttctt tttcccttat gccttatggc cattagtggc ggcgttcaaa gtgagtgata
     3721 tcagtttgga ggaggcttcc cagaatcttg gggcgaaaag ctggttcacg ttcatcttcg
     3781 tcacgttccc gcttgcattg ccgggcgtca tttcgagcgc attgctcatc ttcacggtga
     3841 gcttctcgga tttcggaagt ccgattgtcc tggcgccaaa agaattgaat ctgctcgtcg
     3901 ttgaagcgta ccgggagatt gcaggattct tcaactgggg cggggcggcc atcctgacgg
     3961 tcgtcatggt ggtcgtggcg gcattcttct tctatcttca gcatttattc acgaagggga
     4021 gaaactatgg ctccgtttcc ggaaagccga agcagcagaa gttgattgag aataaattat
     4081 tgacgcgtgt gctatccggc tattccctgt ttgtcgtctt gattccacta ttggcgatgc
     4141 tgtccgtggc gcttcaatcc gttgcgacaa cgtgggggaa ggatttattg ccgagcggtt
     4201 atacattgaa ccactataaa acaatctttt catcgtcgat gggcaatatt cagaacagta
     4261 tcatgctcgc gacaggcgca ttggtgttaa gcgttatcgt cgcaacgttc gtatcgtatt
     4321 tcgttgtgag gcaaaattcg gcgaagctgg atttcatcgc ttccattccg ttggtcgtgc
     4381 cgggcatcgc tttcgggatc gcgctgatcc agacgttcaa tacggcgcct ctccaattga
     4441 cggggacggc tgtgctgttg attatcgctt atacgatccg acggcttcct tatatggtcc
     4501 gttcgacaat gggatcgatg cgagcgatca aacaggatat cgaagaagcg gcggtgaacc
     4561 tcggggcaac cccgctgacc gcggcgatta cgatcgtagg gcctttgatg ctgccaggga
     4621 ttgcagccgg atccgtactt gttttcatta cggtcatcaa agaggcgagt gtttcgatct
     4681 tgcttgcacc gcctgaatgg gcgccgatga gtt
//
LOCUS       DASULI010000021         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_562539, whole
            genome shotgun sequence.
ACCESSION   DASULI010000021 DASULI010000000
VERSION     DASULI010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562539"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFX34_00420"
     CDS             <1..215
                     /locus_tag="VFX34_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003733440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563392.1"
                     /translation="DGNQLIPPFDAIPSLGTNVAKAIVEARKDGIFLSKEDLQQRGRV
                     SKTVIEYMDSLGCLDGMPDSNQLSLF"
     gene            424..900
                     /gene="rimP"
                     /locus_tag="VFX34_00425"
     CDS             424..900
                     /gene="rimP"
                     /locus_tag="VFX34_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX5563393.1"
                     /translation="MSKITEEVEKLVSPIVNDLELELVDIEFVKEGRDWFLRVYVDTP
                     EGNIDIEQCALVSERLSEELDRTDPIPQNYFLEVSSPGAERPLKKEEDFKKAVGQYVF
                     IKTYEPINGMKEFEGYLLSYGPDAAEVEIRIKTRKLQVVIDKEKIAFARLAIDFSA"
     gene            923..2143
                     /gene="nusA"
                     /locus_tag="VFX34_00430"
     CDS             923..2143
                     /gene="nusA"
                     /locus_tag="VFX34_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX5563394.1"
                     /translation="MSSDLLDALTALEKQKGISRDVIVEAIEAALVTAYKRNFNQAQN
                     VRVDLNLEKGTMKVYSRKDVVEDVEDERLQIALEDALAINPAYELGDIVEEEVTPRDF
                     GRIAAQTAKQVVTQRVREAERGLIYDEYVDREDDIVNGIVERLDARNLYVGLGKVEAV
                     LPASEQITTESYQPHDRIKVYITKVERTSRGPQVFVSRTHPGLLRRLFEMEVPEIYDG
                     TVEIKSIAREAGDRSKISVHTNNEEVDPVGSCVGSRGARVQAISNELNGEKVDIVEWS
                     EDPVVFVANALSPSKVLDVQVNEEDRSTTVVVPDYQLSLAIGKRGQNARLAAKLTGWK
                     IDIKSETDARELGIYPPQSDQMGGERLFEDDVYAVTENGNDEYDEDPYFDDELEEESD
                     LEVIDLYSETDEDN"
     gene            2163..2441
                     /locus_tag="VFX34_00435"
     CDS             2163..2441
                     /locus_tag="VFX34_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX5563395.1"
                     /translation="MTSKKKIPLRKCAATGEMLPKKEMIRIVRTKEGEVSVDLTGKKS
                     GRGTYVSKTEEAVETAKRNRSIESQLGTAVPQEVFDDLLHAIRREALK"
     gene            2438..2812
                     /locus_tag="VFX34_00440"
     CDS             2438..2812
                     /locus_tag="VFX34_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HEX5563396.1"
                     /translation="MNPSAIFQLLGMAARARKLITGEELVIKEVRSGNARLVIVSEDA
                     SKNTHKKVNDKCSFYNVEKHVFGSREALGHAIGKESRVVLALTDAGFAKKASGLLNEY
                     NRGWANDEDTCTRIRETSESDE"
     gene            2760..>3163
                     /locus_tag="VFX34_00445"
     CDS             2760..>3163
                     /locus_tag="VFX34_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5563397.1"
                     /translation="MTKIRVHEYAKQVNRTSKEVIEELGKINVNVTNHMSMLDKEATA
                     KLDQRFGKGGSAPSDKSRNASRPAGNAQDKKQGNRPVQGGQRQGQSAQGGNRPAQGGQ
                     RQQSAQGGNRPAQGGQRQGQGAQGGNRPAQGG"
BASE COUNT          942 a          662 c          848 g          711 t
ORIGIN      
        1 tcgatggcaa tcaattgata ccgcctttcg acgcgattcc atccctaggg acgaacgttg
       61 ccaaagcgat tgtcgaagct cgcaaggatg gcatcttctt atcgaaggaa gatctgcaac
      121 agcgggggag agtttcgaaa acggttatag aatatatgga ttcattaggt tgcttggatg
      181 gcatgccaga ttcaaaccaa ctgtctcttt tctaatcgaa gcatcaatcg gagccctccg
      241 cttttgcatc gtctagactc cgggcgccct ccgcttttgc acatcatttg caatgatgcc
      301 cagcagatgg tataattggt gcaacttttg ctatatgtct tgcgaaaaag agtggggttt
      361 cccgctcttt tctgttgttt attcgtataa gacaagtttg aatcaaattt cgggagggtt
      421 atgatgagta aaattacaga agaagttgaa aagctcgtca gtccgatagt gaatgacctc
      481 gagttggaat tagttgatat tgaattcgtt aaagaaggca gggactggtt tttacgggtt
      541 tatgtcgata cgccggaagg gaatatcgat attgaacagt gtgcacttgt gagcgaaagg
      601 ctcagtgaag agctggaccg tacagatcca atcccccaga actattttct agaggtttcc
      661 tcacccggcg ccgaacgtcc gcttaaaaaa gaagaggact tcaaaaaagc agtcggtcag
      721 tatgtattca ttaaaacgta tgaaccgatc aacggcatga aagagtttga gggatacttg
      781 ctttcttacg gtcctgatgc cgcagaagtt gaaatccgca tcaagacccg gaaattgcaa
      841 gtcgttatcg acaaggaaaa aatcgcattt gcacgactag caatcgattt ttccgcataa
      901 ttgaatatag gagtgattaa ttatgagtag tgatctactc gatgcattga cagcccttga
      961 aaagcagaaa ggaatttcga gggatgtaat tgtagaagcg attgaagcgg cccttgttac
     1021 cgcatataaa agaaacttca atcaggcgca aaacgtgcgt gtcgatttga atctggaaaa
     1081 agggacgatg aaagtctatt cccggaaaga tgtcgtggaa gatgtggaag atgaaagact
     1141 tcaaatcgcc cttgaagacg ctttggccat caaccctgca tatgagcttg gagatatcgt
     1201 cgaagaagaa gtgacgccac gcgattttgg ccggattgca gcacagactg ccaagcaagt
     1261 cgtcacacag agagtccgtg aagctgaacg gggtctgatc tatgatgagt atgtagatcg
     1321 ggaagatgac attgtcaacg ggattgtcga gcgactggac gcaaggaatc tatacgtagg
     1381 gcttgggaaa gtggaggcgg ttctgcctgc tagcgaacag atcactacag agtcctacca
     1441 gcctcatgac cgcatcaagg tgtacatcac gaaagtcgaa cgaacttcaa gagggccaca
     1501 agtttttgtt tcccgtacgc acccaggcct tttgcgccga ctgtttgaaa tggaagttcc
     1561 agaaatttat gacggtacgg ttgaaatcaa atcgattgcc cgcgaggcgg gggatcgttc
     1621 gaaaatttcg gtccatacga ataatgagga agtcgatccg gtcggttcat gcgtcggttc
     1681 gcgcggggcg cgcgtccaag ccatttcaaa tgaattgaat ggtgaaaagg tcgatatcgt
     1741 ggaatggtcc gaggatccgg tcgtctttgt cgcgaatgca ttgagccctt ccaaagtact
     1801 ggacgtgcag gtgaatgaag aggacagatc gacaacagtt gtcgtgccgg actaccagct
     1861 atctttggca atcgggaaac gtggccagaa cgcacggttg gctgcaaaac ttacaggatg
     1921 gaaaatcgat attaagagtg aaacggatgc ccgcgagtta ggcatctatc cacctcaatc
     1981 cgatcaaatg ggtggagaac ggctatttga agatgatgta tatgctgtta ccgaaaatgg
     2041 aaacgatgaa tatgatgaag atccgtactt cgatgatgaa ctggaagaag agtccgatct
     2101 ggaagtgatc gatttatatt cggaaacaga cgaagacaat tgagttgaaa ggatgactgc
     2161 ccatgacaag caaaaagaaa atccctttgc gtaaatgtgc ggcgacgggc gagatgctgc
     2221 caaagaagga aatgatccgc attgtccgca caaaagaagg tgaagtttca gtcgatctaa
     2281 caggtaaaaa atcaggacgc ggtacatatg tatcaaaaac tgaagaagct gttgaaactg
     2341 cgaaacgcaa tcgatcgatt gaaagccagc ttggaacagc ggtgcctcag gaagtctttg
     2401 atgatctcct gcatgcgatc cgtcgcgagg cattaaaatg aatccttctg caatcttcca
     2461 attgttaggg atggcagcaa gagcccgcaa gttgatcacc ggtgaagaac tggtcatcaa
     2521 agaagtgcgt tcgggcaatg cgcgtttagt catcgtttct gaagatgctt caaagaatac
     2581 acataaaaaa gtaaatgata aatgtagttt ctacaacgtt gagaagcatg tgttcgggag
     2641 ccgggaagct ctcggccatg caatcggaaa agagtcgcgg gtcgtactcg cgttaacgga
     2701 tgccggcttt gccaaaaaag catccgggct cctcaacgaa tataaccggg ggtgggctaa
     2761 tgacgaagat acgtgtacac gaatacgcga aacaagtgaa tcggacgagt aaagaagtca
     2821 tcgaagagct tgggaaaata aatgtgaatg taacaaacca tatgtcgatg cttgataaag
     2881 aggcgacagc gaaattggat cagcgttttg gaaaaggcgg ctctgctcct tctgacaagt
     2941 ctcgcaacgc atcgcggccg gcaggcaatg cacaggataa aaagcagggc aaccgtccag
     3001 tccaaggcgg acaacgccaa ggccaaagcg cacaaggcgg caatcgaccg gcccaaggcg
     3061 gtcaacgcca acaaagcgca caaggcggca accggccggc tcaaggcggt caacgccaag
     3121 ggcaaggcgc acaaggcggc aaccgaccag ctcaaggcgg tca
//
LOCUS       DASULI010000022         4664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1905394,
            whole genome shotgun sequence.
ACCESSION   DASULI010000022 DASULI010000000
VERSION     DASULI010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905394"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1834
                     /locus_tag="VFX34_00450"
     CDS             <1..1834
                     /locus_tag="VFX34_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5563398.1"
                     /translation="MEVMIMLNTLRVIDNPYQDIPLASVLRAPFVGMTENELAQVRLT
                     AKNKPFYDALKMFMATGGSGVAAETQVKLQRFFLMFEDWRNLARRGSLSELIWQVYND
                     THYYEMVGAMPNGKQRQANLRALHDRAIDYEKTSFRGLFRFLRFVDRMQKRGDDLGAA
                     RFVSETEDVVRIMTIHSSKGLEFPYVFVAGAGRQFNKMDFNEPYLFDQHFGLAVKAID
                     PELRITYTSLPFLAMKEKKELEMRAEEMRVLYVAMTRAKEHLELIATVKDIEKAIGSW
                     QDAQLIEAGEMLPEYTRSRANGYLDWIGPAIARHPDFGKFGFMYGGHPVDDPSKWEIA
                     AYPVSSFLAMEEHEEEIVERPVPASTGDVIEAEWTNEVKRRFDYVYPFMASVEKRSKQ
                     TVSDLKRVALLEMEPDFDDFFTTKDDEVSTPYLHDRPAFMQSRALSGAEIGTAMHTIM
                     QHIDLSKTHTVQDVDRFVTELTTRQLLTMDEANAVDVKAVVQFFGTDLFKRLSKSTRM
                     LKEVPFTYAYDGNDGDHQILQGIADCLFEEKDGWVLLDYKTDRIRGRFRSDEEIQVEM
                     QKRYGIQLSLYKRAVESIARISIKEMVLYLFDGERIVHVQEESV"
     gene            1831..3021
                     /locus_tag="VFX34_00455"
     CDS             1831..3021
                     /locus_tag="VFX34_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX5563399.1"
                     /translation="MTVTPTIGNRIETLDILRGFALLGIFIANMLTFHSPYFYIDPLS
                     YFSTPSDVASYKLINLFVEASFYPVFAMMFGYGLNMQYEKAIANGTSYVPMMARRLAI
                     LMGFGLIHALFIWSGDILFTYALMGFIMLAVVRVSKKWLFWIALIVYLLPTLLFIGGV
                     YVLTVLNPNSLMEGFVDIQQIELAITAYAHGTYGEALVFRITEWLLIGLTNSFLSVFM
                     VLPLIMLGAAFSKWKLFERAAELKGRLAIVGIITLAAGIFVKSWPYIDGFTYYNRLTQ
                     QLIGGPILAIGYACVIILLCTLPIFRTIFRPVSKAGRMSLTTYLMQSIVATLLFYGYG
                     FGLYGKVDIETGTMIAVGIFVIQVILAELWLMKFQMGPFEWLWRKGTYGKNMPKKEEK
                     QQAL"
     gene            3090..3989
                     /locus_tag="VFX34_00460"
     CDS             3090..3989
                     /locus_tag="VFX34_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5563400.1"
                     /translation="MKLLSFRFEGKTLFGPKVKKEEAVWDVKSIAEAFGETDFPSTII
                     EGIAGGLEFVEKVRKLAEQARNSENPEQFKYAFTEIEWLSPIPRTPKNVLGVGKNYAE
                     HAAEMGADAPPEKLLIFTKSPTAIAADEQELPVHADLTESLDYEGELAVVISKKGRNI
                     PKQLAYDHVFGYTIANDLTARNIQTGHKQFFLGKSLDGSCPMGPYIVTKDEIPDAQNL
                     SIVTKVNDEVRQNGNTADMIFKIDELVAEISKFVTLEPGDVILTGTPAGVGKGFNPPK
                     FLKAGDTVKISIEGIGTLVNRFA"
     gene            4195..4569
                     /locus_tag="VFX34_00465"
     CDS             4195..4569
                     /locus_tag="VFX34_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HEX5563401.1"
                     /translation="MDFLTSSTHFHLFTWVVGIIIFLVSAVMANGSKGKKITHMIARL
                     FFVLILISGIALFIKGMDYGRGAEYGIKFLLGLLTIGMMEMVLVRSEKGKKVTTFWIL
                     FFVLLFATMYVGFKLPMGFEFF"
BASE COUNT         1168 a         1028 c         1270 g         1198 t
ORIGIN      
        1 gatggaagtg atgattatgc tcaacacgct tcgcgtcatc gataatccgt atcaggatat
       61 tccgcttgcc tcggttctcc gggcaccgtt cgtcggcatg acggaaaacg agttggcgca
      121 agttcggctg acggcgaaaa acaagccatt ttacgatgcg ctgaaaatgt tcatggcgac
      181 aggcgggtcg ggggtcgctg cagaaacaca agtgaaattg cagcggttct tcctcatgtt
      241 cgaggattgg cgcaatctgg cgcgtcgcgg ttcgttgtcg gagctcatct ggcaagtgta
      301 taacgatacg cattattacg aaatggtcgg cgcgatgccg aacgggaagc agcggcaggc
      361 gaatctgcgt gcgctgcatg accgagccat cgattatgag aagacgtcgt tccgagggct
      421 tttccggttc cttaggtttg tcgacaggat gcagaaacgc ggcgatgatc ttggcgctgc
      481 acgtttcgta agcgagacgg aggatgtcgt ccggatcatg acgatccact cgtcgaaggg
      541 gctcgaattc ccgtatgtgt tcgttgctgg agcgggccga cagttcaata agatggactt
      601 caacgaaccg tatttattcg accagcattt cggcttggcg gtgaaggcga tcgatccaga
      661 attgcgcatt acgtacacat ctcttccttt cctcgccatg aaagagaaga aggagcttga
      721 aatgcgtgcg gaggagatgc gcgttctcta cgtggcgatg acgcgggcga aggagcatct
      781 cgaattgatt gccaccgtga aggatattga aaaggcgatc ggcagctggc aagacgcgca
      841 gctgatcgag gcgggcgaaa tgctgccgga gtatacgagg tcgcgcgcaa acggctattt
      901 ggattggatc gggccagcta ttgcacgcca tccggatttc gggaaattcg gtttcatgta
      961 tggaggccat cccgtcgatg atccgtcgaa atgggaaatt gcggcgtatc ctgtttcatc
     1021 tttcctcgcg atggaggaac atgaggagga aatcgtcgaa aggcctgtac cggcatcaac
     1081 cggcgatgtc atcgaagcgg agtggacgaa cgaagtgaaa cggagattcg actatgtcta
     1141 tccattcatg gcttccgtcg agaaacgctc caagcagacg gtgagcgact tgaaaagggt
     1201 ggcactgctt gagatggagc ctgacttcga cgacttcttc acaacgaagg atgacgaagt
     1261 gagcacgcct tatttgcatg accgtcctgc gttcatgcag tcaagggcgc tgtcgggagc
     1321 ggaaatcggg actgcgatgc atacgatcat gcagcatatc gatctgtcaa agacgcatac
     1381 cgtccaggat gtggaccgat ttgtcaccga gctgacgact cggcaattat taacaatgga
     1441 tgaagcgaac gctgtcgacg tgaaagcggt cgtccaattt ttcgggacag accttttcaa
     1501 aaggctgtcc aaatcgacga ggatgcttaa agaagtgccc ttcacgtatg cgtacgacgg
     1561 gaatgacggc gatcatcaga tcttgcaagg gattgccgac tgcctattcg aagaaaaaga
     1621 tggctgggtg ctgctcgact ataagacgga ccggatccgc ggcagattcc ggtccgacga
     1681 agagattcaa gttgaaatgc aaaagcgtta cggcatccaa ttaagccttt ataaaagagc
     1741 tgtcgagtcg atcgcaagaa tttcaattaa agaaatggtc ctctatttgt ttgatgggga
     1801 gaggattgtg catgttcagg aggaatcagt ttgactgtaa caccgacgat tggaaaccgc
     1861 atcgaaacat tggatatcct gaggggcttt gcgctccttg gcatattcat cgcgaatatg
     1921 ctcactttcc attcaccgta tttttatatc gatccacttt catatttcag cacgccgagt
     1981 gatgtggcgt cctataaatt gatcaatctg tttgtggaag cgagctttta cccggttttc
     2041 gctatgatgt tcgggtatgg attgaatatg caatatgaaa aagcgattgc gaacggaacg
     2101 tcatacgtgc cgatgatggc gaggagactt gcgattctca tggggttcgg cctgatccat
     2161 gcgctgttca tctggtcggg tgatatttta ttcacctatg cattgatggg cttcatcatg
     2221 ttggcggttg tccgggtttc gaaaaaatgg ctcttctgga ttgcgctcat cgtctacttg
     2281 cttccgacgt tgcttttcat cggcggcgtg tatgtattga cggtgcttaa tccgaactcg
     2341 ttgatggaag ggtttgtcga cattcagcaa atcgagttgg ccattacggc ctatgcacac
     2401 ggaacgtatg gcgaagcact tgttttcaga attaccgagt ggctcctcat cggtttgaca
     2461 aactctttcc ttagtgtttt catggtgctg cctttgatca tgctcggggc tgccttctcg
     2521 aaatggaagc tgtttgaacg ggcggcggaa ctgaaaggga gactcgccat tgtcggcatc
     2581 attacgttgg cagccggcat tttcgttaaa tcatggccgt atatcgacgg attcacttat
     2641 tacaatcgat tgacccagca gctgattggc ggcccgattt tggcgattgg ctatgcatgc
     2701 gtcatcatcc tgctttgtac gttgccgatt ttccggacaa ttttccggcc ggtatcaaaa
     2761 gccggtcgca tgtcgctcac gacgtacttg atgcaatcca ttgtcgcgac acttctattc
     2821 tacggatacg gcttcggatt gtatggaaaa gtggatatcg aaacaggaac aatgattgcg
     2881 gtcggcatct ttgtcatcca ggtcatactc gcggagctct ggctaatgaa gttccagatg
     2941 ggccctttcg aatggctatg gcggaaaggg acgtacggaa aaaacatgcc gaaaaaagag
     3001 gagaaacagc aagctttgta gaattgtagt ttgtaatcgg aaaatgtgtt gaaagatttt
     3061 ccaaattatt aggaaggatg ggcttgtaca tgaaactttt gtcgttccgg tttgaaggaa
     3121 aaacattgtt cggtccgaaa gtgaagaaag aggaagcggt ctgggatgtg aaatcgattg
     3181 ccgaggcatt cggcgaaact gatttccctt caacaatcat cgaaggcatt gcaggaggtt
     3241 tggagtttgt cgagaaggtg aggaaacttg ccgaacaagc tcggaatagt gaaaacccgg
     3301 aacaattcaa gtatgcattc acggaaatcg aatggctgtc cccgattccg cgcacaccga
     3361 aaaatgtcct tggcgtcgga aagaactatg ccgaacacgc cgccgagatg ggtgcggacg
     3421 cgccgcctga gaaattgctg atcttcacaa aatcgcctac tgcgattgct gcagatgagc
     3481 aggagcttcc agtgcacgca gaccttacgg aaagcctcga ttatgaaggg gagttggcgg
     3541 tcgtcatcag caaaaaaggg cgcaatatcc cgaaacagct cgcatatgac cacgttttcg
     3601 gctatacgat cgcgaatgat ttaacggcga ggaacataca aactggccat aagcagttct
     3661 tcctcggcaa aagcctggac gggtcttgcc cgatgggtcc ttatatcgta acgaaggatg
     3721 agattccgga tgcgcaaaac ttgtcgatcg tcacaaaagt gaatgatgaa gtccgtcaaa
     3781 atggcaatac agctgatatg atcttcaaaa tcgatgaatt ggtcgccgaa atttcgaagt
     3841 ttgtcacgct tgagccgggc gacgtcattt tgacaggcac gcctgcgggg gtcggtaaag
     3901 gatttaaccc accgaaattc ctgaaagcgg gagatacagt gaaaatttcc atcgagggca
     3961 tcggcacgct cgtcaatcgt tttgcgtaag ctcgtttcct attctggaaa gctattctat
     4021 gataggataa tatagaaatt atctttattc agcaggcgct cagtcgcctg ctaaaaaaag
     4081 ataatgcctc cggcggatgt cacggatttt gaaaggagtt aattgtgcaa gcacaattca
     4141 aaatccggac gcaatttcgc cgaggcgaaa ttgattaatg atgaggtgaa cagtttggac
     4201 tttttgacaa gttcgaccca tttccatctt ttcacatggg tcgtcggcat tatcattttc
     4261 ctcgtttcgg ctgtcatggc gaacgggtca aaaggaaaaa agattacgca tatgattgca
     4321 cgcttatttt tcgtgctcat ccttatttcg ggcatcgcgc tcttcattaa agggatggat
     4381 tacggaagag gcgcagaata cggcattaaa ttcctgctcg gcttgttgac gatcgggatg
     4441 atggaaatgg ttctcgtacg ttccgaaaaa gggaagaaag tgacaacttt ctggattttg
     4501 tttttcgtac tcctttttgc gactatgtat gtgggcttca aattgccgat gggctttgaa
     4561 ttcttctaat attgagcttg cgagccaccg gttactcttc cggtggctcg ttttttcgat
     4621 ttcgcgctca taaaccctgc cgttgcgctc ataaatctca ttcc
//
LOCUS       DASULI010000023         5296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_504286, whole
            genome shotgun sequence.
ACCESSION   DASULI010000023 DASULI010000000
VERSION     DASULI010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504286"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFX34_00470"
     CDS             complement(<1..711)
                     /locus_tag="VFX34_00470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5563402.1"
                     /translation="MDLHKGSLYWPTTYQSIPRKKPAQKADCYDAVIVGGGMSGSLTA
                     LSLLDRGLKIAILDKRRFASGSTAANTGLLQYSNDIMLHELIEQIGRIDAVDFYGNCL
                     QAMGQLKNIADRLPVDPHFICRESIYYASDEDDAERIKKEYRTLSKYGFPCAFWDRDA
                     LLNNAGIDRPCAIVTSGDAEVNPYVFTNGLFDLAEMKGAHLFEFTEMLDVTERSAQLA
                     ISTTEGEMRTKNIIFTTGY"
     gene            791..1687
                     /locus_tag="VFX34_00475"
     CDS             791..1687
                     /locus_tag="VFX34_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX5563403.1"
                     /translation="MTGEQKPHILLIDGMALLFRSFFATSAMGHFFPNDDGVPTNGVQ
                     GFARHALTAASIFNPTHMAVCWDMGAHTFRNDLFDGYKANRPAPAPELVPQFDMTREV
                     SELIGWKNYGIPGMEADDLIGSLVCEWEGKADITIVTGDKDLLQLLRPGVKIAFMKKG
                     YNVYDIYTEERFVEEYEIAPERFVEVKAFTGDTSDGYPGVKGIGPKTALKLVKEYGTV
                     ENVIESLHELTAGMRKKIEEEMEMLHLSKQLAQIHCELEFNDPIEQLSLPDFSEVREP
                     LEEKGYSMILRQLNSLFPELIA"
     gene            complement(1711..2226)
                     /locus_tag="VFX34_00480"
     CDS             complement(1711..2226)
                     /locus_tag="VFX34_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX5563404.1"
                     /translation="MKRLEDFQGKSQYDDEIDKRFQPSACGPVTAFTILRHHLQDSRI
                     QVNALYRLLGGTRIGLFKWRFIRNLRKLLGSEWTIEECGIEEMKQEINAGRPVAAKFD
                     KWFTFHWFSEYTFDYHWVPVVGYKEDENGLILLVHDNGGRNRESRMREIAYEPNRPIL
                     SLIKIAKTTAD"
     gene            complement(2250..2705)
                     /locus_tag="VFX34_00485"
     CDS             complement(2250..2705)
                     /locus_tag="VFX34_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5563405.1"
                     /translation="MDYEQEIEKYKKKIAELEEAIKRSSVPIISSVLDDTILVPIVGY
                     TGSERFELIRTLVLEHLDKHRGVNSVVFDFTAADLNEKEPHDYDSLAIELQMLNNTLK
                     LMDVRPISVGFNPLIVRKIIAAGVQMEFESYVNFRMAMKHLLKEQNATL"
     gene            complement(2826..3143)
                     /locus_tag="VFX34_00490"
     CDS             complement(2826..3143)
                     /locus_tag="VFX34_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5563406.1"
                     /translation="MKSITTVEEFNKVINSEKKSLIKFQAGWCPDCTRMDMFIDPIVK
                     DYDMYTWYDVDRDVLPEIAEKYEVMGIPSLLIFQNGEKKAHLHSANAKTPDQVTDFLE
                     TVK"
     gene            3354..>5296
                     /locus_tag="VFX34_00495"
     CDS             3354..>5296
                     /locus_tag="VFX34_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5563407.1"
                     /translation="MAYQIKKAAVLGSGVMGSGIAAHLANIGIPVLLLDIVPNKLTEQ
                     EEAKGLTLEDQQVRNKFAATALEKLLKQKPAPLAAKQNLSLIEVGNFEDDLEKLKDVD
                     WIIEVIVENLDIKKSLYEKIDTVRTPGTIISSNTSGISIEAMAEGRSEDFQKHFLGTH
                     FFNPPRYLKLLEVIPTKKTDSEVLDFMLKFGEDRLGKGVVVAKDTPNFIANRIGTYGL
                     LVTLREMEKRGYSIGEVDSVTGTLIGRPKSATFRTLDVVGLDTFMHVAKNAYDKTEGE
                     EKEVFELPPFMKKMIDNGWLGAKSKQGFYLKEGKEILELDLETFEYGPTKKLKTPSIE
                     MAKQQKGLANKVKALVYAKDRTGEILWSILSPTLRYSADLIGEIADDIVAIDNAMKWG
                     FGWQQGPFEVWDAIGVAKSVERMKEEGAEIPTFVQQMLDKGVESFYKEEDGDLYFYDG
                     EGYKLVSVNDKAIDLKRYKKKHGVIKKNSGASLIDLGDGIALLEFHSQSNAIGLDIIQ
                     MINFAIDEVEKNFNGLVIGNQGKNFCVGANLAMILMEAQDDNIFELDYTIRSFQNAMM
                     KIKYSRKPVVAAPFQMTLGGGAEVCLPAAHIQASMETYIGLVEVGVGLIPGGGGNVNL
                     YTKHLKGLPNGVHVDYQYVANKV"
BASE COUNT         1491 a         1152 c         1263 g         1390 t
ORIGIN      
        1 atagccggtc gtgaatatga tattttttgt gcgcatttca ccctccgttg tggaaatcgc
       61 caactgggca ctacgctcgg ttacatccag catttccgtg aattcaaata aatgagcacc
      121 cttcatttca gcaagatcga atagtccatt tgtaaataca taaggattca cttccgcatc
      181 cccggaggtc acaatggcgc aaggccggtc gatgccggca ttgttcaata gggcatccct
      241 atcccaaaag gcgcaaggga agccgtattt tgataaggtt cgatattcct tttttatccg
      301 ctcagcgtcg tcctcatcgc ttgcataata aatgctttcc ctgcaaatga aatgtgggtc
      361 caccggcaaa cggtcggcaa tattcttcaa ttgccccatt gcctgtaaac agtttccgta
      421 aaaatcaaca gcatcaattc gcccgatctg ttcaatcaat tcatgaagca tgatatcatt
      481 agagtattgg agtagacctg tattggccgc ggtacttcca gatgcaaaac gtcttttatc
      541 caaaatggcg atcttcaagc cgcgatccag tagggataat gcagtcaggg aaccggacat
      601 tccgcccccg acgatgacag catcatagca atccgctttt tgggcaggct tttttctagg
      661 aatggattga taggtagttg gccaatacaa tgaaccttta tgtaaatcca taaaaatacc
      721 cccttgcctt tccataccca tcccatatgg tttggaaaag tgaagtatat tattttagga
      781 gatgatagca atgaccggtg aacaaaaacc acatattttg cttatagacg gcatggcttt
      841 attattcaga tcgttttttg caacttctgc aatggggcac ttcttcccga acgacgatgg
      901 agtcccgaca aatggcgttc aagggtttgc ccgccatgca ttgacggcag cttcgatatt
      961 caaccctacc catatggcgg tctgctggga tatgggtgcg catacgttca gaaatgatct
     1021 tttcgacgga tataaagcga accgtcctgc acctgcacct gaacttgtcc cgcaattcga
     1081 tatgacacgg gaagtgtccg aactgatcgg ttggaagaat tacggaatcc ctggaatgga
     1141 ggccgacgat ctgatcggct cgcttgtctg cgaatgggag ggcaaggcag atattacaat
     1201 cgttacaggg gataaggacc ttttgcagct gttgcgtcct ggcgtcaaaa tcgccttcat
     1261 gaaaaaagga tacaatgtat acgatattta tactgaagaa cgatttgttg aagaatacga
     1321 gattgcacca gagcggttcg tcgaagtgaa ggcgtttacg ggagatacga gcgacggcta
     1381 tccgggggtc aaagggattg gcccgaaaac ggcgttgaaa ctcgtgaagg aatacggtac
     1441 tgtcgaaaat gtcatcgagt cattgcatga actgacagca ggcatgcgga aaaagatcga
     1501 agaggaaatg gaaatgctgc acctgtcaaa acaacttgca caaatccatt gcgagcttga
     1561 attcaatgat ccgatcgagc aattaagcct tccggatttc tctgaagtga gggaaccatt
     1621 agaggaaaaa ggctattcca tgatcctgag acaattgaac tcattatttc cagagctgat
     1681 tgcttaatag aggaagaaaa accactgcta ctagtcggca gtggtttttg cgatttttat
     1741 gagtgaaagg ataggccggt taggttcata tgcgatttca cgcatgcgac tttcacggtt
     1801 ccgccctcca ttgtcatgga caagcaaaat aagtccgttt tcatcttctt tataaccgac
     1861 gaccggtacc caatgataat caaaagtata ttcgctgaac caatgaaacg taaaccattt
     1921 atcgaacttt gctgcaactg gccgacctgc attgatttcc tgtttcatct cttcgatgcc
     1981 gcattcttcg atcgtccatt cggaaccgag cagcttgcga agattgcgga tgaagcgcca
     2041 tttgaacagg ccgatccggg tcccgccgag caaccggtat agagcattca cttgaatgcg
     2101 ggaatcttgc agatggtgtc ggagaatcgt gaaggcagtt accggtccgc atgcggaagg
     2161 ctggaaacgc ttatcgattt cgtcatcgta ttgtgatttg ccttggaaat cttccaaccg
     2221 cttcatttgc agatccctcc actgatagat tacagcgtag cgttttgctc tttcaggaga
     2281 tgtttcattg ccatcctgaa attgacatac gattcaaatt ccatttggac gccagctgct
     2341 atgatctttc tgacaatcag cggattgaag ccgactgaaa tcgggcggac atccatcagt
     2401 ttcaacgtat tgttcagcat ttgcaattca attgcgagcg agtcataatc atgcggttcc
     2461 ttttcattca aatcggccgc tgtaaagtcg aatacgacac tattcacgcc tcgatgttta
     2521 tccagatgct ccaagacgag cgtgcggatc agttcaaacc gctctgatcc cgtatagccg
     2581 acgatgggca caagaattgt atcgtctaga actgatgata tgattggaac ggaggatctt
     2641 ttgatcgctt cctccaattc ggcaattttc tttttgtatt tttctatttc ttgttcgtaa
     2701 tccatttggc acctcagcat atactttttc tttcattata cgataattag atagaaaaga
     2761 ggcatattaa aggaggagac ccccataggt tgccccctcc acaaattatc gttatgattc
     2821 cggaattatt tcacggtttc aaggaaatcg gtcacttgat ccggtgtttt tgcatttgcg
     2881 ctatgtaagt gcgctttctt ttccccattt tggaagatga gcaagctcgg aattcccatc
     2941 acttcatatt tctccgcgat ttcaggcaac acgtcacggt ccacgtcata ccatgtatac
     3001 atatcatagt ctttgacgat cggatcgatg aacatatcca tgcgggtgca gtccgggcac
     3061 cagccggcct ggaacttgat aagcgatttt ttttcgctgt taatgacctt gttgaattct
     3121 tctactgtag taattgattt catattgaac acactccttt actatgtagt gtaccttctt
     3181 tttatgaatc aacatagaaa aatgcccata acaaaaaata gggaataaat gcatttcaat
     3241 attgcgaaaa agaagagaat gatatagact gatagatata gataacgttt ctattttttt
     3301 tactcgcaaa atgaatgagt gttcattcaa aagaaaaagg gaggcttcgc aacgtggcat
     3361 atcaaatcaa gaaagcggca gtattaggtt caggcgtaat gggatctggc attgcagccc
     3421 atctcgccaa tatcggcatt cctgtccttc tgctggacat cgttccaaat aagttgactg
     3481 aacaggaaga agcaaaaggc cttacattgg aagaccagca agtccgcaat aaatttgcgg
     3541 cgacagcttt ggagaaactt ttgaagcaga agccggcgcc attggccgct aaacagaatc
     3601 tttcacttat tgaagtcggc aatttcgaag atgacttgga aaagttgaaa gacgtggact
     3661 ggatcattga ggtcatcgtt gagaatttgg acattaaaaa aagtttgtat gaaaaaatag
     3721 atacagttcg gacaccgggt acaattatca gctccaacac atcgggaatc agcatcgaag
     3781 cgatggcgga agggcgttca gaggatttcc aaaagcattt ccttggcacc cacttcttca
     3841 atccgcctcg ttatttgaag ctgcttgaag tgattccgac gaaaaagacg gattccgaag
     3901 ttctggattt catgctgaag ttcggagagg atcgcttagg gaagggtgtt gtcgtcgcaa
     3961 aagacacacc gaacttcatt gcgaaccgta tcggaacgta cggtcttctc gtaacgctcc
     4021 gtgaaatgga gaagcgcggc tattcaatcg gagaagtaga ctcggtgacc ggcacgctga
     4081 ttggccggcc gaaatcggca actttccgta cattggatgt cgtcggtctc gatacgttca
     4141 tgcatgttgc aaaaaatgcg tatgacaaaa cggaaggtga agagaaggaa gtgttcgagc
     4201 ttccgccgtt catgaagaaa atgatcgaca atggatggct cggcgcgaaa tcgaaacaag
     4261 gattctattt gaaggaagga aaagaaatcc ttgaacttga cctggaaacg ttcgaatatg
     4321 gcccgacgaa aaaattgaaa acgccttcaa ttgaaatggc gaagcagcaa aagggtcttg
     4381 cgaataaagt gaaggcactc gtatacgcaa aagaccggac gggagaaatc ctatggagca
     4441 tcctttcgcc gacattgcgc tattccgcag acttgatcgg tgaaatcgcc gacgacatcg
     4501 tcgcgatcga taatgcgatg aagtggggct tcggctggca gcaaggtcca tttgaagttt
     4561 gggatgcgat cggtgttgcg aaatcggttg agagaatgaa agaggaaggt gcagaaattc
     4621 ctactttcgt ccaacagatg ctcgataaag gcgtggagtc tttctataag gaagaagacg
     4681 gtgatcttta tttctatgat ggagaaggct acaagcttgt ttccgtcaat gacaaagcga
     4741 ttgacttaaa acggtacaag aaaaagcacg gcgtcatcaa gaagaactcc ggcgccagcc
     4801 tgatcgatct cggagacggc atcgcgttat tggagttcca ttcccaatcg aatgcgattg
     4861 gtctcgatat catccaaatg atcaatttcg caatcgatga agtggagaag aatttcaatg
     4921 gcctcgtcat cggaaaccaa gggaagaact tctgtgtcgg cgcgaacctg gcaatgattt
     4981 tgatggaagc gcaggatgac aatatttttg aactggatta cacaatccgc tcattccaaa
     5041 acgcgatgat gaaaatcaaa tatagccgaa agccggttgt tgcggcgcca ttccaaatga
     5101 cgctcggagg cggggcggaa gtatgcttgc cagcggcgca tatccaagca tcgatggaaa
     5161 cgtatatcgg cttggtcgaa gtcggcgtcg gcctgattcc tggaggaggc ggcaacgtca
     5221 atctgtacac gaagcatctg aaaggtttgc cgaacggcgt gcacgtcgat taccaatatg
     5281 ttgcgaataa agtgtt
//
LOCUS       DASULI010000024         7103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_134251, whole
            genome shotgun sequence.
ACCESSION   DASULI010000024 DASULI010000000
VERSION     DASULI010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134251"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2215)
                     /locus_tag="VFX34_00500"
     CDS             complement(<1..2215)
                     /locus_tag="VFX34_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5563408.1"
                     /translation="MKSKYEFIEHHKNLPINVFVFEKADEYASWHKEIELLFIAQGNA
                     VINVKNETHSLRAQDVLLVNSHTLHRIADVAQGTVILSLVIDPYFFEVYYEGFSKLHF
                     ECNSSGAYGLDKSYAYIRKQLTNMMIRFLTPTPHYQFELLSDASDLVTYMLTHFKSGV
                     SSMSGLSDEHLHQRFERILDYLNEHYDKKITLADLAKKEYISSYYLSRLFSEHLGIGF
                     SKYLNTLRLNRSMYDLIHTNKNLMEIAISHGFTSTKAYRDTFKEKYEMTPTEYRKRID
                     TKEDAPMDVLATPVSHVELLRLLTSLQGTDENAGQLPAISEIVISDTLPQIKQWNTLE
                     KILYFTYAVDELKLNWQASLGKIHEEIQFDYIRFSGIFNNGMYFYDETEGTYNWFNVE
                     TILDFFISKNIRPFIKLTYNEQEFTIPEWHKMLEHFISHCIKRYGYANVREWKFEFAH
                     LDRSYSKMIELYTKSLDHFQATFERLHFGIEFFPADNFHEEHFLKHFFNKPLSFISVV
                     MTEEIYDRLRGFVTILFNSIHRKMGVKTYFIKTEKANYLNDTCYAANKIIHNTLHEHH
                     GVSAPVSFMDHAKSTALFHGEPGLLTFNGLKKPIYNAYVLLDKLKGYVLSRGTHHYAV
                     KNQDHIRILLFTKLNPAEVRERVKYYEKHKVPKAAKRSIVHLNLQLQKGNYKLKSYEL
                     NREHGSIFDEWIRMGQPKNFDHEELEFLKSKEFMRLTSSTAEVEGELQLQHSIPEN"
     gene            2332..4068
                     /locus_tag="VFX34_00505"
     CDS             2332..4068
                     /locus_tag="VFX34_00505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/carnitine O-acyltransferase"
                     /protein_id="HEX5563409.1"
                     /translation="MENTQQLLPSLPIPALDETKSKLLEWIEPLVSEEQFEQTKRTAD
                     RFFGEEGEARVLHEQLIEWDGQLPGSWLKPFWDEMYLAFRGSLPLEMNFNMLLNTDGF
                     HEDALPSRIGKISYLVTELYHSIVDGEVKPEMVKGTPLCMSQYENLFKSIRIPRTGMD
                     EYIVGELTKADNHIVLVHRNQFYQVGVSDASGNPYSAGQLESVVETILASSPEWGENI
                     GSFTTADRETAADVYAELSKSAMNEKNLQAIADALVVISIDETETGNALESLFSGSAN
                     RYYDKTIEIIVSENGEVGFNFEHTGIDGTTALAVVNQISAGLQRDRSGTGEAAGQPNV
                     SLLEWTINDLIKTVLRRLEKQFEKAKEEYDLNLLEFNEFGAGEIKRLGISPDAFFHMA
                     LQIAQYRTFDAIRSTYEPVAVRFFNEGRTECARAASNEKSALVMALESGDESNAALYE
                     LMKQASDAHSRRLKECQQGRGVERHMYGLLQMAKMHGMSELPELFKDTGYTTLRHDFL
                     STSGLSIPQVKSWIFGPVVADGYGIGYSIVDDHISINISSKIGNGEHAETLVRHVASA
                     LNELRAIAETQQ"
     gene            complement(4216..4620)
                     /locus_tag="VFX34_00510"
     CDS             complement(4216..4620)
                     /locus_tag="VFX34_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563410.1"
                     /translation="MYYSSFPYGFDPRQQAYMPVHPGYMAMPTPTPQGGRSPFPPVNT
                     RKFNESAKRFKQVLNEANLFIGALTASDDFARRLMDAAQRSDQKEVERLVRTTGVTIK
                     YNLSYTPSGIRIDFSNSDAQNSCCMLRMALGW"
     gene            complement(4686..5885)
                     /locus_tag="VFX34_00515"
     CDS             complement(4686..5885)
                     /locus_tag="VFX34_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010289049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5563411.1"
                     /translation="MYKLYCRSFQRVMKVSTFFLPWREPHLLEGENSLAELPMLIKRE
                     GLRTVLIVTDKGISATGLMDGFLAGLQNVGIRFVIYDKTVPNPTIDNIEEALQLYKTN
                     NCQGLIAFGGGSPIDCAKGVGARLARPNKTIPQMKGILKVNKKMPPLFAVPTTAGTGS
                     EATLACVVSNSETHEKYALMDTALIPHFAVLDPLLTVKLPKPITSTTGMDALTHAVEA
                     YIGKSNTKQTRKWSIDAVKLIFGNLYEAYSNGENITARTNMQKAAYLAGMAFTRAYVG
                     NVHAIAHTLSGFYNVPHGLANAVILPHVLDYYGESVHAPLADLADAAGVCAPSDSTRE
                     KAMKFIQAIKELNEKMDIPSKVDCILDSDIPLMAERAIQEANPLYPVPKILRKEDMDK
                     LIRTIGA"
     gene            6099..6614
                     /locus_tag="VFX34_00520"
     CDS             6099..6614
                     /locus_tag="VFX34_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563412.1"
                     /translation="MQKKIRVLLLITGLVLFKIWIWDVYIVNSTADAALDGAEGEAGV
                     NYVISKNKELAIRPYEEGFKIYAVSKGFWGWSVTDEMFIEDDGIVERGLQFIGNEKVY
                     LSLIVDKEQSLDKVIGYSTNVGTIHFNRTVGDHAILYYYYSDQPMGEVTYEGTRLNGK
                     TEKLPRDKELD"
     gene            6662..>7103
                     /locus_tag="VFX34_00525"
     CDS             6662..>7103
                     /locus_tag="VFX34_00525"
                     /inference="COORDINATES: protein motif:HMM:NF025633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4362 domain-containing protein"
                     /protein_id="HEX5563413.1"
                     /translation="MPKKFYSFLLLGLCLLAACSYDSEKAVKNGDVINMNGPIYNFDR
                     FEQFMDHVEAKETASVRIANYTLEGNPTLYNLSFDGSVFDLEIDRSKDKERGDNPRKM
                     NLSCTDLVSEEGQQLFIYALEGCQSDVDRFLLINILKAQIEDHEH"
BASE COUNT         1822 a         1527 c         1790 g         1964 t
ORIGIN      
        1 cgttttcagg aatcgaatgc tgcagctgga gctcgccttc cacctctgcc gtggagcttg
       61 tcagacgcat gaattctttg gatttgagaa attccagttc ttcatgatcg aagtttttcg
      121 gctgtcccat gcggatccat tcatcaaaga tggaaccgtg ctcccggttc aactcatacg
      181 atttcagttt gtagttccct ttttgcaact gcaggttcaa atgaacaatt gaacgcttcg
      241 ccgcttttgg aactttatgc ttttcgtagt atttcacccg ttcacgcact tccgctggat
      301 tcagctttgt gaaaagaagg atgcggatat gatcctggtt tttcacagca taatgatgcg
      361 tcccgcggga tagcacatag cctttcagct tatcgagcaa aacataagca ttatagatgg
      421 gcttctttag tccgttgaac gtcaataatc caggttcgcc gtggaataat gccgtcgact
      481 ttgcatgatc catgaagctg acgggagctg aaaccccatg gtgttcatgg agcgtgttat
      541 gaatgatttt attcgccgca tagcaagtgt cattcaagta attcgccttt tcggttttga
      601 tgaaatatgt ttttacaccc attttccgat ggatggaatt aaataaaatc gtaacaaatc
      661 ctctaagtct atcgtatatt tcttcggtca tgacaaccga gatgaaactg agcggcttat
      721 tgaaaaagtg cttcaaaaaa tgctcttcgt ggaagttatc ggccggaaag aattcgatgc
      781 cgaaatggag cctctcgaaa gtggcttgaa agtgatccaa gcttttcgta tagagctcaa
      841 tcattttact atagcttctg tccaaatggg caaattcaaa tttccattcc cggacgttgg
      901 cgtatccata ccgtttgatg cagtggctaa taaaatgctc gagcatttta tgccactcgg
      961 gaatggtgaa ctcctgctcg ttatacgtca acttgatgaa aggccttata tttttcgaaa
     1021 tgaaaaagtc caggatcgtt tcaacattga accagttata cgttccttcg gtttcatcgt
     1081 aaaaatacat cccgttattg aagatgccgc tgaagcggat gtaatcaaat tgaatttcct
     1141 cgtggatctt tccgagactg gcttgccaat tcaatttgag ttcatcgact gcataggtga
     1201 aatacaggat cttctcgagt gtgttccatt gtttaatttg cgggagtgta tcggaaatca
     1261 cgatttcact aatggcagga agctgtccgg cgttttcgtc cgtgccttgc aatgaagtga
     1321 gcagccgcaa taattcaaca tggctgaccg gtgtcgctag cacatccatc ggtgcgtctt
     1381 ccttcgtgtc gatccgcttc cggtattcag tcggggtcat ttcatatttc tctttaaacg
     1441 tatcacggta ggctttcgtg gaagtgaatc cgtggctgat cgcgatttcc atcaggtttt
     1501 tattcgtatg gatcaggtcg tacatggatc ggttcaggcg cagcgtattt aagtatttgg
     1561 aaaagccgat gcccagatgt tcggagaaga gacgggacaa ataatagctg ctgatgtatt
     1621 cttttttagc caagtcggca agtgtgattt ttttatcgta gtgttcattc aaataatcca
     1681 atatgcgctc gaaacgttgg tgcagatgct cgtcggaaag gccgctcatg ctggaaaccc
     1741 cgctcttgaa atgcgtcagc atgtaagtga cgaggtccga tgcatcggat agcagttcaa
     1801 attgatagtg gggtgtcgga gtcaaaaacc ggatcatcat gttggtgagc tgtttccgga
     1861 tatatgcgta ggatttatcc aatccgtatg caccgctcga gttgcattcg aagtgaagct
     1921 tggagaagcc ttcataatac acttcgaaga aataagggtc gatcactaaa gacaggatga
     1981 cggtgccttg tgcaacgtcc gcgatgcgat gcagcgtatg gctgttcacg agcagaacgt
     2041 cttgcgcacg aagcgagtgt gtctcgtttt tgacgtttat gaccgcattg ccttgtgcga
     2101 tgaatagaag ttcgatttct ttatgccacg aagcgtattc gtccgctttt tcaaatacga
     2161 atacatttat cggtaagttc ttatgatgtt caataaattc atatttgctt ttcaagggat
     2221 tcccttcact ttcgttgact ttatttgtaa aatcaaaatt atttattact tgtttattgt
     2281 atcacgctca tttgtcgttg cctatttcta ttaaaaggag acttgcctca tatggaaaat
     2341 actcaacaac tattgccttc gttgccaata cctgcacttg atgagacaaa atcgaaactg
     2401 ctggaatgga tcgagccgct tgtcagtgag gagcaattcg agcagacgaa aaggacagcg
     2461 gacaggtttt tcggtgaaga gggtgaggcc cgagtgctcc atgaacaatt gatcgagtgg
     2521 gacggacaat tgccggggag ctggctcaaa ccgttttggg acgagatgta tttggcgttc
     2581 aggggttcat tgccactgga aatgaatttc aatatgctgc tgaatacgga tgggtttcat
     2641 gaggacgcat tgccgtcccg tatcgggaag atcagttatt tggtgacgga gctgtaccat
     2701 tcgatcgttg atggggaagt taagccggaa atggtgaaag ggacccctct atgcatgtcc
     2761 caatatgaaa acttattcaa gtccatccgg attccgcgta caggtatgga tgaatacata
     2821 gtcggcgagt tgacgaaggc ggacaatcat attgtccttg tgcaccgcaa ccagttctat
     2881 caagtgggtg tttcagatgc aagcgggaat ccatactccg ccggacagtt ggaaagtgtg
     2941 gttgaaacga tcctcgcatc ttcgccggaa tggggggaga atatcgggag cttcaccact
     3001 gccgatcggg aaacggcagc agacgtctat gcggaacttt cgaagtcggc gatgaacgaa
     3061 aagaatcttc aagctattgc ggatgcactt gtcgtcattt cgattgatga aacagagaca
     3121 ggaaatgcat tggaaagtct gttttcaggc agtgcgaatc gatactatga caaaacgatt
     3181 gaaatcatcg tttcggaaaa tggcgaggtc ggcttcaact tcgagcatac cggaatcgac
     3241 ggaacaacag ccttggctgt cgtcaatcaa attagcgccg gattgcagcg ggataggtcc
     3301 gggactggtg aagcggcagg ccaaccgaat gtctctttgt tggaatggac aattaacgat
     3361 ttgataaaaa cggtgctacg ccggctggag aagcaattcg aaaaggcgaa agaggagtac
     3421 gacttgaacc tgctggagtt caatgagttc ggggcagggg aaatcaaaag gctcggcatc
     3481 agtccggatg cattcttcca tatggcgctg cagattgctc aataccgaac gttcgacgcc
     3541 atccgaagca cgtatgagcc ggtcgccgtg cgcttcttca acgaaggaag gacggaatgt
     3601 gcaagggcgg caagcaatga aaagtctgct ttggtaatgg ctctggagag tggggatgaa
     3661 agcaacgcgg cgctttatga actgatgaag caagctagcg atgcgcactc ccgcagacta
     3721 aaagaatgcc agcagggccg cggtgttgaa cgacatatgt atggccttct gcaaatggct
     3781 aagatgcacg ggatgagcga gttgcctgaa ctattcaagg atactgggta tacaacgctc
     3841 cgtcatgatt tcctatcgac gagcggcctg tcgattccgc aggtgaaatc gtggattttc
     3901 ggaccggtcg ttgcagatgg ttacgggatc ggctattcaa ttgtcgatga tcatatttcc
     3961 atcaacatct ccagcaaaat tgggaatggg gaacatgcgg agacattggt cagacatgtt
     4021 gcttcggcgt tgaatgaatt aagggcaatt gcagaaactc aacagtgatt ttaatgcaca
     4081 ccaacccatc gggcgatgac gggttggtgt ttttttaagt tatcgatcat ttcccgtctt
     4141 ttatggtcat ttcgttacct ttggcatagc tgtatagcga aaaaagaagc cgtcgtttga
     4201 ctggcttcca tgttttcacc aacccagcgc catccgcaac atgcagcaac tgttttgggc
     4261 atcgctgttc gaaaaatcga tccggatgcc ggagggtgtg taggatagat tatatttgat
     4321 ggttacacct gtcgtgcgga cgagccgctc cacttccttt tggtcagaac gctgagcggc
     4381 gtccatgagt ctgcgggcga aatcatctga agccgtcagt gcgccgatga atagattcgc
     4441 ttcattcaac acttgtttga accgtttggc ggactcattg aatttccgcg tattcaccgg
     4501 aggaaaaggg cttcttccgc cttgcggtgt cggtgtgggc atcgccatat atcctgggtg
     4561 gactggcatg taagcttgtt gccggggatc aaagccgtat ggaaaagaag aataatacat
     4621 aggcaacctc cgcatttctt cgatgcctca gtatatgccc attgcggaag acgcgtgttt
     4681 ttccgtcaag ccccgatcgt ccggatcagc ttatccatat cctctttacg taaaatcttc
     4741 ggcaccgggt aaagcggatt cgcttcctgg atcgcacgct ccgccatgag cgggatatcg
     4801 ctgtcgagaa tgcagtccac tttcgacggg atgtccatct tttcattcaa ttctttgatt
     4861 gcttgaatga atttcatcgc cttctccctg gtggagtcgg aaggtgcgca aacgcctgct
     4921 gcatccgcca aatccgccaa aggtgcgtgc acggattccc cgtaataatc gaggacatgt
     4981 ggcaggatga cggcgtttgc gagtccgtgc ggcacattgt aaaacccgct tagcgtatgg
     5041 gcgatggcat ggacattgcc cacatacgcg cgtgtgaacg ccattcccgc aagataggcg
     5101 gctttttgca tattcgttcg cgcggttatg ttttcgccat tggaataggc ttcgtaaaga
     5161 tttccgaaaa tgagcttcac ggcatcgatg ctccattttc tcgtctgctt cgtattgctt
     5221 ttgccgatat acgcttcgac cgcatgcgtc agcgcgtcca tcccagtcgt tgacgtaata
     5281 ggcttcggaa gtttcacggt gagcagcgga tccagcaccg cgaaatgcgg aatgagtgcg
     5341 gtatccatta gggcatattt ctcatgggtc tcgctgttgg aaacgacgca ggcaagtgtc
     5401 gcttcactgc cggtcccggc cgttgtagga accgcgaata gaggaggcat cttcttattc
     5461 accttcaata tgcctttcat ttgaggaatc gtcttgtttg gtctggccaa ccgtgcaccg
     5521 acgcctttcg cacaatcgat cggcgaccca ccgccgaatg cgatgagccc ttggcagttg
     5581 ttcgtcttgt agagctgcaa cgcctcctcg atgttatcga tcgtcggatt cgggaccgtc
     5641 ttgtcgtata tgacaaacct gattcctaca ttctgcaagc ctgcgaggaa gccgtccatt
     5701 aacccggtcg cggaaattcc tttatccgtg acaatcaaca ccgttctaag gccttcgcgt
     5761 ttaatcaaca tcggaagctc cgctaaacta ttttctcctt caagtaaatg aggctcccgc
     5821 cacggcaaga aaaaggtaga tactttcatc accctctgga agcttcggca atacaatttg
     5881 tacatgttca ttcaccatcc cttcaagtag atgtaacatt ctaactatta taccgtgaat
     5941 tgggagtatt ttgctagaga tcatttttcg actctgccac ttgaagaaga tatcgatcat
     6001 ttcccgaatt gcgtagaaaa cacttaaatg gaatcaggga attccaacat tttggcgtag
     6061 taagagtttt aaggtaagat ttgagagagg tgaccgctat gcaaaagaaa atacgagttt
     6121 tgctgctcat cacggggctt gttttattca aaatctggat ttgggatgta tacatcgtaa
     6181 actccactgc cgatgcagcg ttggatgggg cggaagggga agcgggtgtc aattatgtca
     6241 tcagcaaaaa caaggagctt gccattcggc cttatgaaga agggttcaaa atatatgcgg
     6301 tgtccaaagg attttggggt tggtccgtca cggatgaaat gtttattgag gacgatggaa
     6361 tagtagaacg gggtttgcaa tttataggga atgaaaaagt atatttgagt ttgatagtgg
     6421 ataaagagca gtccttggat aaagttatcg gttactccac gaacgtcgga acgatccatt
     6481 tcaatagaac tgtcggtgat catgcaattc tttactatta ttacagtgat cagccaatgg
     6541 gtgaagtcac ttatgaaggc acccgattaa acggaaagac ggagaagttg ccacgggata
     6601 aggagctaga ttgacgcata tagtgtaggc aaaacacgaa gtacgtcaat ggaggaatct
     6661 tatgccgaaa aagttctatt cctttttgct gcttggatta tgcctattag ccgcttgttc
     6721 gtatgatagt gaaaaagcgg ttaaaaatgg ggatgttata aatatgaacg gacccattta
     6781 caattttgat cggtttgaac aattcatgga tcatgtcgaa gcgaaagaaa ccgcctccgt
     6841 ccggattgcg aattacacgc ttgaaggaaa tccgacctta tacaatttgt ctttcgatgg
     6901 ttccgtattc gatttggaaa tcgatcggtc gaaagataaa gagcgagggg acaaccctcg
     6961 taaaatgaat ttgtcctgta cggatcttgt atcggaggag gggcagcaat tattcatcta
     7021 tgcgctggaa ggatgtcagt ctgatgtcga tcgtttcctt ctcataaata tcttgaaagc
     7081 acaaattgaa gatcatgagc att
//
LOCUS       DASULI010000025         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_270302, whole
            genome shotgun sequence.
ACCESSION   DASULI010000025 DASULI010000000
VERSION     DASULI010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270302"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2390
                     /locus_tag="VFX34_00530"
     CDS             <1..>2390
                     /locus_tag="VFX34_00530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEX5563414.1"
                     /translation="IQNDTFVRDLIMMAQDIMEVPAIIRKDTAPEDRKRIELHAHTTM
                     SQMDAVTSASALVAQAAKWGHPAVAITDHANVQSFPEAYSAGKKHGIKVLFGLEANLV
                     DDGVPIVYDEQHRELENDTFVVFDVETTGLSAVYDTIIELAAVKVVNGELKERFERFA
                     NPHHPLSSTTTELTGITDDMVKDAPEVSEVIKDFKEFIGDAILVAHNASFDMGFFYEA
                     SKKAGVHDKNYPVIDTLELARFLYPEMRNHRLNTLAKKFDIELTQHHRAIYDTEATAY
                     LFIRLMKEAATEKGIVYLDDFNKNIGEGDAYKRSRPSHCTLLATDDEGLKNLFKLVSI
                     SHMNYFYRVPRIPRSLLMKYRKGLLVGSGCDKGEVFEGLMQKSMEEVEEIAGFYDYLE
                     LHPKPAYSHLIELELIRDEWNLEDIMRKMIKLGKKVGLPVCATGNVHYIDETDAMYRQ
                     VLVRSQGGANPMNRHSLPEVHFRTTDEMLEHFSFLGQEVAEQIVIDNPRAVADRVGEV
                     KVIKDDLYTPTIIGADDEVRNLTYSMARSIYGEQLPEIIEARIEKELKSIIDNGFAVI
                     YLISHKLVKKSLDDGYLVGSRGSVGSSLVATMMEITEVNPMPPHYVCPSCKLSEFFDD
                     GSVGSGFDLPDKACTSCGTMFKKDGQDIPFETFLGFNGDKVPDIDLNFSGEYQAHAHN
                     YTKELFGEDFVYRAGTIGTVAEKTAYGYVRGYMNDNNLHLRGAEIDRLVQGCTGVKRN
                     TGQHPGGIIVVPDNMEIFDFTPIQFPADDTASSWRTTHFDFHSIDNNLLKLDILGH"
BASE COUNT          671 a          533 c          607 g          579 t
ORIGIN      
        1 gcattcagaa cgatacattc gtccgtgatc tcattatgat ggctcaggac atcatggagg
       61 ttcccgcgat tattagaaaa gatactgctc cagaggatcg caaacggatc gaattgcatg
      121 cacatacgac aatgagtcag atggatgcgg tgacttcggc atccgcctta gtcgcacaag
      181 cggcaaaatg gggccatcct gcagttgcaa ttaccgacca tgcgaacgtc caatcgttcc
      241 cggaagccta ttctgccggc aagaaacatg gcataaaggt gctattcggc ttggaagcga
      301 atttggtcga cgacggcgtc ccgattgtct atgacgagca gcaccgggag ctcgaaaatg
      361 acacattcgt tgtatttgac gtggagacga ctgggctgtc cgccgtatac gacacaatca
      421 tcgaattggc cgcagtcaaa gtagtcaatg gtgagctgaa ggaaaggttt gaacggtttg
      481 cgaatccaca tcatccattg tcttctacaa caacggagct tacgggcatt acagatgaca
      541 tggtgaagga tgcacctgaa gtgtcagagg tcataaagga cttcaaggaa ttcattggag
      601 atgcaatcct ggtcgcacat aacgcatctt tcgatatggg cttcttttac gaggcaagta
      661 aaaaagccgg tgtgcatgac aaaaattacc cggtcatcga tacgcttgag ttggcacgtt
      721 tcctttaccc ggaaatgcgg aatcacagat tgaatacgtt agccaaaaag tttgatattg
      781 aactgacaca gcatcaccgt gcgatctacg atacagaagc gacagcctat ctcttcatcc
      841 gcctcatgaa ggaagcggct acggaaaaag gtatcgtgta cttggatgat tttaataaaa
      901 acatcggaga aggggatgcc tataaacgct caaggccatc ccattgcaca ttacttgcta
      961 cggatgatga aggtttgaaa aatctattca aactcgtctc catttcccat atgaattact
     1021 tttacagagt tccacgcatc cctcgttctt tattgatgaa atatcggaaa ggcctcctag
     1081 tcgggtcggg ttgcgacaaa ggcgaagtct tcgaaggcct gatgcagaag tctatggaag
     1141 aggttgaaga aattgcgggc ttctatgatt atcttgaatt gcatccgaaa cctgcctatt
     1201 cccacttgat tgaactggaa ttgatcaggg atgaatggaa tctggaagat attatgcgca
     1261 agatgatcaa gctcgggaaa aaggtcggac ttcctgtatg tgcgacaggg aatgtccact
     1321 atatagacga gacggatgct atgtaccgtc aagtccttgt ccgttcccaa ggcggggcaa
     1381 atccgatgaa tcgacactca ttgcctgaag tccatttccg gacgacagac gaaatgctcg
     1441 agcacttctc atttttagga caagaagtcg ctgaacaaat cgtcatcgac aatccgcggg
     1501 cagtggcgga ccgtgtcgga gaagtaaaag taatcaaaga tgatctgtat acgccaacga
     1561 ttatcggagc agatgatgaa gttaggaatc tcacctattc aatggcacgc agcatttacg
     1621 gtgagcagct gcctgaaata atcgaagcac ggatagagaa agaattgaaa tcgattatcg
     1681 acaatggctt cgcggtgatc tacctcattt cacataagct tgtgaaaaaa tcattggatg
     1741 atggatacct tgtcggttcc cgtggatcag tcggctcctc cctcgtcgct acgatgatgg
     1801 aaattacaga agtaaacccg atgcctccgc attacgtatg tccatcttgc aagctatcgg
     1861 agttcttcga tgatggttct gtcggctccg ggttcgactt gccggacaaa gcatgcacgt
     1921 cgtgcggtac gatgttcaaa aaagatggac aagatatccc gtttgaaaca ttcctcggct
     1981 ttaacgggga taaagttccg gatatcgact tgaacttcag tggggaatac caggcgcatg
     2041 cgcataacta cacgaaagag ctctttggtg aagacttcgt gtaccgtgcg gggacgatcg
     2101 gaacggttgc tgaaaagacg gcatacggct atgtcagggg ctatatgaat gacaataacc
     2161 tgcatttgcg tggagcggaa attgaccggc tcgtgcaagg ctgtacgggc gtcaaacgga
     2221 atacagggca gcacccggga gggatcatcg ttgtcccgga taatatggag atttttgatt
     2281 tcacgccgat tcaattcccg gcggacgaca cggcttcaag ttggcggacg acccatttcg
     2341 acttccactc gattgataat aacctactga aactggacat attgggacat
//
LOCUS       DASULI010000026         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_717458, whole
            genome shotgun sequence.
ACCESSION   DASULI010000026 DASULI010000000
VERSION     DASULI010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717458"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /gene="prfB"
                     /locus_tag="VFX34_00535"
     CDS             <1..862
                     /gene="prfB"
                     /locus_tag="VFX34_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5563415.1"
                     /translation="MTLELLREEADEELHEELVSELKEFKKKMEDFDLQMLLSDEFDS
                     SNAVLEIHSGAGGTESQDWASMLLRMYTRWAEDQGYKVETLDYQAGDEAGVKSVTLSI
                     KGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFSSVEVMPEFDGDIDIEIRTEDLKI
                     DTYRSSGAGGQHVNTTDSAVRITHIPTGAIVTCQTERSQIKNRERAMNLLKAKLYQLK
                     VEEEEARLAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNEETGNVGAVMDGEIDMF
                     INAYLRSKIS"
     gene            1260..1583
                     /locus_tag="VFX34_00540"
     CDS             1260..1583
                     /locus_tag="VFX34_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013399986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5563416.1"
                     /translation="MKNKLVAVMFGAALVLGACGGGNNNNNAGGGETAGVDADKVFQA
                     NCATCHGGNLEGRGNAPALANIGSQLSESEIHDIIENGKNGMPGGLISGEELDAVAKW
                     LAEKK"
     gene            1917..2603
                     /gene="ftsE"
                     /locus_tag="VFX34_00545"
     CDS             1917..2603
                     /gene="ftsE"
                     /locus_tag="VFX34_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX5563417.1"
                     /translation="MIVMKDVYKKYPNGVVAANGINVEIGRGEFVYVVGPSGAGKSTF
                     IKMMYREESPTKGDIIINGINLATLRNKRVPYLRRQIGVVFQDFKLLPKLNVYENVAF
                     ALEVIEETPKQIRKKVNDVLALVGLTQKARMFPNELSGGEQQRVSIARSIVNVPKVVI
                     ADEPTGNLDPDTSWEIMKIFEQINDRGTTIVMATHNKEIVNTIRHRVIVVDGGMITRD
                     EYEGVYGYES"
     gene            2593..3477
                     /gene="ftsX"
                     /locus_tag="VFX34_00550"
     CDS             2593..3477
                     /gene="ftsX"
                     /locus_tag="VFX34_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX5563418.1"
                     /translation="MKARTMGRHLRESLKSLGRNSWMTFASVSSVTVTLLLVGVFIVI
                     MMNLNQLADNLENDVEIKVITEPAADAEAVKVLEEQVRKTPGVSEVVHATKDEELEKM
                     IKSFGDELDLYKQNNPLGDALYVKATDPHKTAEVARKIDTYDNTYQVVYGEGKIEKLF
                     NVLTMSRNIGMVLILALLFTAMFLISNTIRLTIVARGREIEIMKLVGATNSFVRIPFV
                     LEGIWLGVLGSIIPMVVISVSYYELYSQWQPRLQNELFQLLNATPFILQVNALLLFMG
                     VFIGVWGSFMSVRKFLRV"
     gene            3577..>4603
                     /locus_tag="VFX34_00555"
     CDS             3577..>4603
                     /locus_tag="VFX34_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEX5563419.1"
                     /translation="MNKRRWIMASFVIVFLMTSITGGTGALASTLSDLKKEQKQYQQK
                     KYEIKSDMDKKTNAIKTNKTKIEKIMGQIDALNKEIEETNANMNRVIDKINKTNEEID
                     ALKESIADLEKKIAERDVVLRERVRAMQVKGNDVSYVDVLLGANSFADFIDRFSAVST
                     LMDADRDIMRRQKEDIEQLEAEKALVEKKLAELKESKAKLERLKANLESQKKAKGKLI
                     DELEAEQERLSKEKQALEEEYHETIEVEAELEKKVIAEQKRIAEIARREAERKRKERE
                     AAAAAARPGALPHVSNGFWTKPANGTYSSSFGWRVHPIYNTKRQHRGADIAAPIGTSV
                     VAAGDGVV"
BASE COUNT         1426 a          952 c         1192 g         1033 t
ORIGIN      
        1 gatgacacta gaattgcttc gtgaagaagc cgatgaagag cttcatgagg aattggtttc
       61 agagctaaaa gaattcaaaa agaaaatgga agacttcgac ttgcaaatgc tattgagcga
      121 tgagttcgac agcagcaatg ccgtccttga aatccattca ggcgcgggcg gaacggagtc
      181 ccaagactgg gcttccatgc tgctgcgcat gtatacaaga tgggctgaag accaaggata
      241 taaagtggag acacttgatt accaggcggg agatgaagcg ggcgtgaaat ccgtgacact
      301 ttccatcaaa ggccataatg catacggcta tctgaaagcg gaaaaaggcg ttcaccgtct
      361 cgtccgcatt tcgccgttcg attcatccgg ccgtcgtcac acttcgttct cttcagtgga
      421 agtgatgcct gagttcgacg gagacatcga tatcgaaatc cgtacggaag acttgaagat
      481 cgatacgtac cgttcaagcg gcgcgggcgg tcagcatgtc aacacgacgg attccgcagt
      541 acggatcacg catattccga caggtgctat cgtgacgtgt caaacggaac gttcacaaat
      601 caagaaccgg gagcgtgcga tgaacctcct gaaagcgaag ctctaccaac tgaaggtaga
      661 agaggaagaa gcgcgtctag ctgaaatccg gggcgagcag aaggaaatcg gctggggaag
      721 ccagattcgc tcctacgtgt tccatcctta ctccatggtg aaagatcacc gtacgaacga
      781 ggagacagga aacgtcggcg ccgtcatgga cggggaaatc gacatgttca tcaacgcgta
      841 tctgcgttcg aaaatttcat aatgaatgaa gaaaaagcgc actccggtgc gcttttttgt
      901 ttagcaccgg atatcggtca tgccacggcg gatatcgatc aaattcacaa atttatcgat
      961 caaaacaggt tcagttatcg atcattacct ataacatatc ggtcaatttc acaaagttat
     1021 cgtcatttca ggacctttca cccactgcac ctacactgca agctccaaca aaaaaggttt
     1081 gaaattccga tttacgtcaa tagtaaaagg taaaggcaaa tgggataaat ccgcctggtg
     1141 aaaaactttc caatttgtca tgaatttgat aaatatggac gcctcccctt gcggtgtaca
     1201 atcccgatcc tttggtatac taaggaatgt aggataattt tttgacagga ggaaacaaca
     1261 tgaagaacaa gctagtagca gtcatgtttg gcgcggcgct tgtacttggt gcatgcggcg
     1321 gcgggaacaa caacaacaat gccggcggag gagaaactgc cggtgttgac gcggacaaag
     1381 tcttccaagc caactgtgca acttgtcatg gcggcaatct tgaaggacgt ggaaatgctc
     1441 cggcattggc gaacatcgga tcacagcttt ccgaaagtga gattcatgat attatcgaaa
     1501 atggtaaaaa tggcatgcct ggcggtttga tcagtggtga agaattggac gctgttgcaa
     1561 aatggcttgc tgaaaagaaa taatggattt gaaaaacgca ttggtcatca ccggccattg
     1621 cgttttttgt tttgaataaa atgtggaaat tcactccaga actattgcag gaatgttaca
     1681 aagcagcgat ttaagcacca aaacgaatac attaaattaa atgtacgata catattttca
     1741 tagtgaaaaa gggaagctgg cgcatataat gccgatttac cgcactcggt caaaagtact
     1801 gttgaattgt gtcgggacaa atgaaacata tctgtaatac ttagcaagcc tatacatgtt
     1861 ataatgaatt tgtcgacgaa aaatgtggaa tacaatacga ttcaggtggt ttttacatga
     1921 ttgtaatgaa agatgtttat aagaaatacc cgaacggggt cgttgctgca aatggcatca
     1981 acgtcgaaat cggtcgaggc gagtttgttt atgtcgttgg accgagcgga gcggggaaat
     2041 cgacgttcat taaaatgatg tatcgcgagg aatccccgac aaaaggcgac ataatcatta
     2101 acgggataaa tcttgcaacg ttaagaaata agagagtccc ctatttgaga aggcagatcg
     2161 gcgtcgtctt ccaagacttc aaactgcttc ctaaattgaa tgtttacgag aatgtggcat
     2221 tcgcacttga agtgatcgag gaaacaccga agcagatccg caaaaaagtg aatgatgtat
     2281 tggcactggt cggattgacg caaaaggcga gaatgttccc gaacgaactg tcgggcggag
     2341 aacagcaacg ggtttccatt gcgcggtcaa tcgtcaatgt ccccaaggtc gtcattgcgg
     2401 acgagcctac tgggaactta gatccggata catcttggga aatcatgaag atttttgaac
     2461 agatcaatga ccgtggcacg acaatcgtca tggccacgca taataaagag atcgtaaata
     2521 caatcaggca tcgggtcatc gtcgtcgatg gtgggatgat tacgagagat gaatacgaag
     2581 gggtatacgg ttatgaaagc taggacgatg gggcggcatc tcagagaaag ccttaaaagc
     2641 ctcggccgga acagctggat gacgttcgcc tctgtcagtt cggttacagt cacccttctg
     2701 ctcgtcgggg tgttcatcgt gattatgatg aacttgaacc aattggcaga taatcttgaa
     2761 aacgatgtag agatcaaagt catcacggaa cctgcggcag atgccgaggc tgtgaaagtg
     2821 ttggaggagc aagtccggaa aacaccgggt gttagcgagg tcgtccatgc gacaaaggac
     2881 gaagaactgg aaaagatgat caaatccttc ggggatgaat tggaccttta caagcagaac
     2941 aacccattag gggacgccct ctatgtaaaa gcgacggatc cccataagac agccgaagtc
     3001 gcccgtaaga tcgacaccta cgacaacacg tatcaagtcg tctatggtga agggaagatc
     3061 gagaaattgt tcaatgtgct caccatgagc agaaacattg gaatggtact catcttggcc
     3121 ctattattca ctgctatgtt cttaatttcg aatacgatcc gtctcacaat tgttgcgaga
     3181 ggaagagaaa tcgagattat gaaactagta ggcgccacga atagcttcgt acggattccg
     3241 ttcgtactgg aagggatttg gctcggcgta ctcggatcga tcattccgat ggtcgtcatt
     3301 tccgtttcgt attatgagct ttattcacaa tggcagccaa ggttacagaa tgaattattc
     3361 caattgttga atgcaacacc tttcattctc caagtgaacg cccttcttct attcatgggc
     3421 gtgtttatcg gcgtatgggg cagcttcatg tctgtccgga aatttcttag agtctgacag
     3481 gctgtcacgg aatacgggga acgctgtagt ccccggtaac agtctgattg tgaacgaatc
     3541 aacctgcagc actagctaaa agggggaaaa gagaagttga acaaacgtag atggatcatg
     3601 gcgagctttg tcatcgtctt cctgatgacg tcgattactg gcggaacagg ggcattggcg
     3661 agcacattaa gcgatttgaa gaaggaacag aagcaatacc agcaaaaaaa gtatgaaatc
     3721 aagtccgata tggacaagaa aacaaatgcc atcaagacaa ataaaactaa aatcgaaaag
     3781 atcatgggtc agattgatgc tttgaataaa gaaatcgaag aaacgaacgc aaacatgaac
     3841 cgcgtcatcg ataaaataaa caaaacgaat gaagaaatcg atgcgttgaa ggaatccatc
     3901 gcggatcttg aaaagaagat cgctgaacgt gacgtcgtcc ttcgggagcg cgtgcgggcc
     3961 atgcaagtga agggcaacga tgtaagctat gtagatgtac ttctcggggc aaacagcttc
     4021 gcggatttca tcgaccgctt ctccgccgtc tcaacgctga tggatgcaga ccgcgacatc
     4081 atgcggagac agaaggaaga tatcgagcag cttgaagctg aaaaagcgct tgtcgaaaag
     4141 aaactggccg agttgaagga aagcaaagcg aagctcgaaa ggctgaaagc gaatctcgaa
     4201 tcccagaaaa aagcgaaggg caaactgatt gacgaattgg aagccgagca agaaaggctt
     4261 tcaaaagaaa agcaagcgtt ggaagaagag tatcatgaaa caattgaagt agaagcggaa
     4321 cttgaaaaga aagtaatcgc tgaacagaag cgtattgcgg agatcgcccg tagggaagcg
     4381 gaacgcaaac ggaaagaaag agaggcagcc gcggcagcag caagacctgg agcattgccg
     4441 catgtatcaa atggcttctg gacgaaaccc gcgaatggaa cctattcatc ttcattcggt
     4501 tggcgggtac acccgatcta taatacaaag cgtcaacacc gtggagcgga tattgccgca
     4561 ccaatcggaa catcggtcgt tgcagcaggg gacggagtcg ttt
//
LOCUS       DASULI010000027         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_815433, whole
            genome shotgun sequence.
ACCESSION   DASULI010000027 DASULI010000000
VERSION     DASULI010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815433"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_binding    410..643
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            697..1854
                     /locus_tag="VFX34_00560"
     CDS             697..1854
                     /locus_tag="VFX34_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011067018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5563420.1"
                     /translation="MLQDQYHLRIPGPTPIPPSVQRAMARPMIGHRGNETSELLHSIK
                     PRLKKVFGTEQDVMIVTGSGTSGLEAAVVNAVKPLDEVLVIVTGAFGARFVAICEAYG
                     IRVHKYEVEWGKAFDPLKIKEHLRSIPNITAVFATYCETSTGVMNPIHELAAAVNDVS
                     DALIIVDGVSCVGGVETKMDDWGVDILVTGSQKAFMLPAGLMFIAASERAWEVIRSNP
                     NRGFYLDLLKYKDDLAKDTTPFTPALSILFGLDQALELMEKEGLENVYGRHALMKEMV
                     RGAFRALEIPLLTNDEDASETVTAVKPDDFDAEEFRKVINKEFRLSVAGGQERLKGKI
                     FRIGHMGYCSPSDVLQTLSMIEIGLIKSGKNIELGKGVAAAQRIYMENGGE"
     gene            1858..>2488
                     /locus_tag="VFX34_00565"
     CDS             1858..>2488
                     /locus_tag="VFX34_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX5563421.1"
                     /translation="MTYHILIADPLSEEGIHPLRQVDGLEITIATDLSTDKLLERIKD
                     FDALLVRSQTQVTREVIEAASNLKIIGRAGVGVDNIDLDAATEHGIIVVNAPDGNTNS
                     AAEHTIAMLTALARKIPQAFNSLKNGEWDRKSFIGVELKNKTLGVIGLGRIGAEVTAR
                     AKGQRMNVIAYDPFLTEEKAQKMGIGFGTVDDVLEAADFITIHTPLLKET"
BASE COUNT          677 a          503 c          640 g          668 t
ORIGIN      
        1 aacgcccagc atccaagcac ttcgccataa tgcagtggct cttccttcgg atttccattt
       61 aaaataccca atgttctccc tcatttcatt ttcttttcca ttgtgaatgg aactgctttt
      121 atgatgtgta aacttcggta ttttatgcgg ttatttggtt ttcattaatt ttaattgaca
      181 ttttaaaaat gtttgataag atacgtaaca atattatttt tggcgaagca gcttttgaga
      241 acgccgaaca aatgacgaac gaatttcagt atatttatta ttttgttcgc attttcaatg
      301 attttattat cggcaacata tgactttgag tattttttta gaaatacacg aataaacagt
      361 tgacatttcg aaaccctgtg atatgattgt caataattaa ataactcgat taatgattta
      421 gagggagaca ggctttgcgt atcggaatcc atagagagct gatggttggt gggaatcagt
      481 gtttctgacg tatggcagca ctcctgaatc ggtggactga aacttcttcc tagttagttc
      541 agtaggtcca cccggaacat gcccgttatg catgcaatga aggctgtcgt catgacagcg
      601 aaaaagggtg gcaccgcgta gctgatggct tttcgcccct tgcatctagt tgcaagggac
      661 ggaaggcttt tttatatttc taaggaggag attgacatgt tgcaagatca gtatcattta
      721 cgtattccag gacctactcc cattccgcca agtgttcagc gggcgatggc acgaccgatg
      781 atcggccatc gagggaatga gacgtccgaa ttgcttcact ccattaaacc acgtttaaaa
      841 aaagtgtttg ggacagaaca ggatgtcatg attgttacgg gaagtggtac ttccggattg
      901 gaagcggctg ttgtcaatgc ggtgaagccg ctggacgagg tgcttgtcat tgtcaccggc
      961 gcattcggcg cgcgtttcgt tgcaatttgc gaggcatatg gaatccgtgt ccacaaatat
     1021 gaagtcgaat ggggcaaagc ttttgaccct cttaaaatca aggaacatct tcggagcatc
     1081 ccaaacatca cggccgtctt tgcaacgtat tgtgaaacat cgacaggtgt catgaatccg
     1141 atccatgaac ttgcagcagc agtgaatgat gtatccgatg cgctcatcat tgtggatggt
     1201 gtttcctgtg taggcggcgt ggaaacgaaa atggatgatt ggggcgtcga cattctggtg
     1261 acaggttccc aaaaagcgtt catgctgcca gccggactga tgttcatcgc agcaagtgag
     1321 cgggcatggg aagttattcg ctccaatccg aaccgcggtt tctatttgga tttattgaag
     1381 tacaaagatg atctggcaaa ggatacaact cctttcaccc ctgccctttc cattcttttc
     1441 ggtctcgatc aagcgcttga attgatggaa aaggaaggac tggaaaatgt gtatggccgt
     1501 catgcactca tgaaagagat ggtgcgtgga gctttcaggg cacttgaaat ccctttattg
     1561 acgaatgatg aagatgcgtc ggagacagtc actgcggtta agccggatga ttttgatgcg
     1621 gaggaattcc ggaaagtgat caataaagaa ttcagactat cggtcgcagg agggcaggaa
     1681 cgattaaagg ggaaaatctt ccgcatcggg catatgggct actgctcccc ttccgatgtt
     1741 ttgcagacac ttagtatgat cgaaattggg ttaattaaat ccggaaagaa cattgaactc
     1801 ggcaaaggtg ttgcggctgc gcagcgaatc tatatggaaa atgggggaga ataaccgatg
     1861 acatatcata tattgatagc cgatccgcta agcgaggaag gaatccatcc gctacggcaa
     1921 gtggatggat tggaaattac gattgccact gacctgtcaa cagataaact gcttgagaga
     1981 atcaaagatt tcgatgcttt gcttgtccgc agccagacgc aagtgacacg ggaagtgatt
     2041 gaagctgcca gtaatctgaa gattatcggg cgtgcaggtg tcggagtcga taatattgac
     2101 ctggatgccg ccacggagca tggcatcatt gtcgtcaatg cgcctgatgg caatacgaat
     2161 tccgcagccg agcatacaat tgcgatgttg acggctctgg cgagaaaaat cccgcaggca
     2221 ttcaactcat tgaaaaacgg cgaatgggat cgcaaatcat tcatcggtgt cgagttgaaa
     2281 aacaagacgc tcggcgttat cggactcgga aggatcggtg cggaagtgac cgcccgtgcg
     2341 aaggggcagc ggatgaatgt tatcgcgtat gacccgttct tgactgagga aaaggcgcaa
     2401 aaaatgggaa tcggtttcgg gacagttgac gatgtgttgg aagcggctga tttcattaca
     2461 atccacactc ctttattgaa agagacac
//
LOCUS       DASULI010000028         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1515963,
            whole genome shotgun sequence.
ACCESSION   DASULI010000028 DASULI010000000
VERSION     DASULI010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515963"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFX34_00570"
     CDS             <1..708
                     /locus_tag="VFX34_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5563422.1"
                     /translation="QAGERIALVGANGSGKSTLFRMMLGQEEFDGDIWLTKGMSIGYM
                     SQAVLDLPEDVTLAEYFHADTFQEQGLIRTQLTNLGFGEKHWRLPLGQLSQGEKVKVK
                     LMQFIQEGTDVLLLDEPTNHLDLPSREQLEKALATFPGTLLFASHDRYFVERMADSLL
                     VFENGGIGKLPMTLLEWEEQRNAPPKQPSVEERLRVETELQAVLGKLSMLKAGDGEYS
                     KLDREFQELSRKLRELK"
     gene            763..888
                     /locus_tag="VFX34_00575"
     CDS             763..888
                     /locus_tag="VFX34_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563423.1"
                     /translation="MAQSPAVMLEIGPVVLEFDQVMLEFKLVMAQSPAVMLVLGP"
     gene            1003..2199
                     /locus_tag="VFX34_00580"
     CDS             1003..2199
                     /locus_tag="VFX34_00580"
                     /inference="COORDINATES: protein motif:HMM:NF023011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3238 domain-containing protein"
                     /protein_id="HEX5563424.1"
                     /translation="MLFEIKSVKHEHDLISFTWNDVGGFYNVYKDGKHLYEGTVPEFQ
                     DGDFKHGKLYHYMIERVENNQVVDVIAMQTSAFAEHKNVENPLQLLVMTTIVAKTQIA
                     LSWEGIKDVKEYEVYRNGKLMAKVDTNRFIDRDIDADQSYVYTIRSRRPLKQSEELLT
                     SVKSAISNLFGMLNSATSTSESAVETFTSIKSIGKPNNLLKPINEREQEIAAVDRWKF
                     RYTTFLADKWVRNPNPLSPNHFFEGDQRGFDPNGKGFRTRVDIELAYDEKGEPMTFTK
                     EVGPTVAYDHLKRFREKAKASADGITMQRSDHQQGESGFLLEHTVGNPLTTAPKIDYE
                     VNAVFRRHGTFDMSGYFDPAPHHEIYLMKGRGEAWQPIIQVESRGLAFMSEAIAYHYW
                     RCSNFE"
     gene            2298..>2545
                     /locus_tag="VFX34_00585"
     CDS             2298..>2545
                     /locus_tag="VFX34_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016086794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEX5563425.1"
                     /translation="MKHIFKQGTDKSKPTLLLLHGTGGTEQDLLPLAQLIDPEASVLS
                     VRGNVLENGMPRFFRRLAEGVFDEEDLIFRTEELHTFL"
BASE COUNT          689 a          525 c          734 g          597 t
ORIGIN      
        1 caggcgggtg aacggattgc gcttgtcggt gcgaatggaa gcggcaagtc gacgctgttc
       61 cgcatgatgt tagggcagga ggagtttgac ggggatattt ggctgacgaa agggatgtcg
      121 atcggctata tgagccaggc agttctcgac ttgcccgagg acgtcacgct tgccgagtat
      181 tttcacgcgg atacgttcca ggagcaggga ttgatccgca cacaattgac gaatctcggt
      241 tttggcgaaa agcattggcg cttgccgttg ggtcaattga gccaaggtga aaaggtgaaa
      301 gtgaagctga tgcagttcat ccaggaaggg acggacgtgc tgttattgga tgaaccgaca
      361 aaccatctgg atttgccatc gagggagcag cttgaaaagg cattggcgac atttccggga
      421 acgcttctat tcgcctcgca tgatcggtat ttcgttgagc ggatggcgga cagcttgctc
      481 gtttttgaaa atggaggcat cgggaaattg ccgatgacgt tgctggaatg ggaggaacag
      541 cggaacgcgc caccgaagca gccgtccgtc gaagaacgac tgcgggtgga aacggaattg
      601 caggcggttt tagggaaatt aagcatgctg aaggccgggg acggggagta ttcgaaattg
      661 gaccgggaat ttcaggagct aagcaggaaa ttgagggagt tgaaatgata cagcccgctt
      721 cggcacgtgc cggggcgggt tttcttatgg ggaggaaatg ttatggcgca atctccggca
      781 gttatgctcg agattggacc ggttgtgctc gagttcgacc aagttatgct cgagttcaaa
      841 ctagttatgg cgcaatcccc ggcggttatg ctcgtcttgg gaccttaggc tctcccccaa
      901 acattgtttc aggacacctt aacaagggca ttgacaagaa aaagccttgt aatgtttagg
      961 cgattggcca atgcagcaaa atgaaagtag gaggtggtcc gtatgctatt tgaaatcaaa
     1021 tcggtgaagc atgagcatga cctgatttca tttacatgga atgatgtcgg gggattttac
     1081 aatgtgtata aggacggcaa gcatttgtat gaagggaccg ttccggaatt ccaggacggt
     1141 gatttcaaac atggcaagct gtatcactat atgatagagc gggttgagaa caaccaagtc
     1201 gtcgatgtga tcgcgatgca gacatcggcc ttcgccgaac ataaaaatgt cgaaaacccg
     1261 ttgcaattgc ttgtcatgac gacgattgta gcaaagacgc aaattgcgct gtcgtgggag
     1321 ggaataaaag acgtcaagga atatgaagtt taccgaaatg ggaagttgat ggcgaaagtc
     1381 gacacgaaca gattcattga ccgagacatt gatgcggatc agtcatacgt ctatacgatc
     1441 cgttccaggc gtccgctgaa acaatcggag gaactgctga cgtcggtgaa atcggcaata
     1501 tccaatctat tcggcatgtt gaattccgcc acatcgactt cggaatcggc tgttgaaaca
     1561 tttacatcga ttaaatcgat cggcaagcct aacaatctgc tgaagccgat caatgaacgt
     1621 gaacaggaaa ttgctgccgt tgatcggtgg aaattccgtt acacaacatt ccttgcggat
     1681 aaatgggtac gaaatccaaa cccgctgtca ccgaatcatt tttttgaagg tgatcaaagg
     1741 gggtttgatc cgaatgggaa aggcttccgg acacgtgttg atattgagct tgcctacgac
     1801 gagaaaggcg agccgatgac gttcacgaaa gaagtggggc caaccgtcgc ctacgaccat
     1861 ctgaagcgtt tcagggaaaa ggcgaaggca tcagccgatg ggataacgat gcaacggtcc
     1921 gaccatcaac aaggggaaag cggttttctg ctggagcata ctgtcggcaa tccgctgaca
     1981 accgcgccga aaatcgatta tgaagtgaat gcagtgttca gaaggcatgg cacattcgat
     2041 atgtcgggtt atttcgatcc ggcgccccat cacgaaatct atttgatgaa agggagaggg
     2101 gaagcctggc aacccatcat acaagtcgaa agcagggggc tcgccttcat gtcggaagcg
     2161 atcgcctacc attattggcg ttgctcgaac tttgagtgaa acagcccttt tgacaacaaa
     2221 gttggactct gttattgtta tcttgaattc gagacaatat ggtttgcttt actttaagaa
     2281 acaaaggggg agccactttg aaacatatat ttaaacaagg aaccgacaaa tcgaagccga
     2341 cgctgctttt attgcatgga accggcggaa cggaacagga tttattgccg cttgcgcaat
     2401 tgatcgatcc agaagcttcc gtactaagtg tgcggggaaa tgtgctggaa aacgggatgc
     2461 cccgattttt cagacggctc gcggaaggtg tttttgacga agaggatttg attttccgga
     2521 cggaagaatt gcatacgttt ctgga
//
LOCUS       DASULI010000029         7625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_308822, whole
            genome shotgun sequence.
ACCESSION   DASULI010000029 DASULI010000000
VERSION     DASULI010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308822"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /gene="ispF"
                     /locus_tag="VFX34_00590"
     CDS             complement(<1..322)
                     /gene="ispF"
                     /locus_tag="VFX34_00590"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5563426.1"
                     /translation="MIRIGQGFDVHQFEEGRPLIIGGITIPYEKGLTGHSDADVLLHT
                     VTDAALGAIGEGDIGRHFPDTDEAFKDADSAILLQKVWEIVESRGYRLGNVDCTIMAQ
                     KPKMA"
     gene            complement(319..1017)
                     /gene="ispD"
                     /locus_tag="VFX34_00595"
     CDS             complement(319..1017)
                     /gene="ispD"
                     /locus_tag="VFX34_00595"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5563427.1"
                     /translation="METYTVLLPAAGSGKRMGAGFNKLFLEMAGKPILIHTLDVFERD
                     PDCSGMILSVKYEEKPQIEEMLERFGITKVAALVEGGAERQQSVAACINAHQSGGIVL
                     VHDAARPFIKRDVVRKLVEVALEEGAAIAGVQVKDTMKVAPDGIVEETVDRSKLWAIQ
                     TPQAFRYEILKEASDKAEAEGFLGTDESMIVERLGHPVRVVESTYDNVKMTTKEDLLF
                     GEVLLKQRRQEDFQ"
     gene            complement(1040..2143)
                     /locus_tag="VFX34_00600"
     CDS             complement(1040..2143)
                     /locus_tag="VFX34_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEX5563428.1"
                     /translation="MLKRVVQIGLLLIGGTLGVLFLPYLFTLSSFTSKPMVDNPYVAA
                     VLGALILYLLSLFIADPIVNFIKWMEDRLLKAPIFDLLFGTIGLMVGLSVAFLVSFGL
                     SSLEIPFITSVLPVVLSILLGYLGFQVGFKKREEFSHAIFAARNSSPKKRDGAEAAGQ
                     SDAKHHKILDTSVIIDGRIADIASTGFLEGLLVVPQFVLTELQNIADSSDSLKRTKGR
                     RGLDVLKRLQTDDGPSILITDEDFADVAEVDLKLVRLAKSMNGQVVTNDFNLNKVADL
                     HGVAVLNINDLANAVKPVVIPGEEMHVAVIKDGKEHNQGVAYLDDGTMIVIEDGRVHI
                     GNAIDVIVTSVLQTSAGRMIFAKPKNGKTAKAY"
     gene            complement(2326..3696)
                     /gene="radA"
                     /locus_tag="VFX34_00605"
     CDS             complement(2326..3696)
                     /gene="radA"
                     /locus_tag="VFX34_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5563429.1"
                     /translation="MAKRKTKFMCRDCGYESPKWMGRCPGCGEWNTMDEEVEIVSKGP
                     RGAFQHTETIQQKAMPISAIETMEEPRVKTELGELNRVLGGGIVPGSLVLIGGDPGIG
                     KSTLLLQVSSLLANQKQRVLYISGEESIRQTKLRAERLGVASDELFIYAETDLAKIHE
                     TINDVKPKFVIADSIQTIHHPEVTSAPGSVSQVRECTAELMRIAKTQNIAIFIVGHVT
                     KDGQIAGPRLLEHMVDTVLYFEGERHHTYRILRSVKNRFGSTNEIAIFEMLQSGLKEV
                     LNPSELFLRERSQGGAGSTVVASMEGTRPILVEIQALVTPSSFNYPKRMATGIDQNRV
                     SLLMAVLEKRMGMLLQAQDAYIKVAGGVKLDEPAIDLAVLVSIVSSYRDAAAGATDCF
                     IGEVGLTGEVRRVSRIEQRVTEAAKLGFEKVIIPSSNIGGWDFPEGIQVVGVETISEA
                     LNIAFS"
     gene            complement(3766..6213)
                     /gene="clpC"
                     /locus_tag="VFX34_00610"
                     /gene_synonym="mecB"
     CDS             complement(3766..6213)
                     /gene="clpC"
                     /locus_tag="VFX34_00610"
                     /gene_synonym="mecB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpC"
                     /protein_id="HEX5563430.1"
                     /translation="MMFNRFTQRAQKVLQLAQEEAIRLKHESIGTEHILLGLIREGGG
                     IAAKALEAIDVSFDTIEKGVEGLVGSGSKEVGPIVHYTPRAKKVIELSVDESRKLGHS
                     YIGTEHILLALIREGEGVAARVLNNAGVSLNRARQQVLQLLGSNDSSVGNQGASSSAS
                     TPTLDSLARDLTEVAREGTLDPVIGRSKEITRVIEVLARRTKNNPVLIGEPGVGKTAI
                     AEGLALQIVNNEVPEILRDKRVMTLDMGTVVAGTKYRGEFEDRMKKVMEEIRQAGNII
                     LFIDELHTLIGAGGAEGAIDASNILKPSLARGELQCIGATTLDEYRKYIEKDAALERR
                     FQPIQVDEPTVEETILIIKGLRDRYEAHHRVKITDEAIEAAAKMSDRYISDRFLPDKA
                     IDLIDEAGSKVRLRSYTTPPNLKELEAKLEAIRHEKNAAVQSQEFEKAASFRDKEQKM
                     KEELETTKTEWKEKQGKKESEVTVNDIAEVVAMWTGVPVAKIAETETAKLLNMEELLH
                     ERVIGQKEAVTAISRAIRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAEAMFGD
                     EDAMIRIDMSEYMEKHATSRLVGSPPGYVGYEEGGQLTEKVRRKPYSVVLLDEIEKAH
                     PDVFNILLQVLEDGRLTDSKGRTVDFRNTVVIMTSNVGAETLKSNRYVGFNLQDGERD
                     YEDMKSTMLEALKKAFRPEFLNRVDEMIVFHSLEKEHLREIVTLMSNELTKRLAEQDI
                     TLELTDAAKLKISEEGYDPDYGARPLRRALQKHVEDRLSEELLSGTVLTGGKVIVDVE
                     DGEFVVKTDRVAVTEKQ"
     gene            complement(6392..7474)
                     /locus_tag="VFX34_00615"
     CDS             complement(6392..7474)
                     /locus_tag="VFX34_00615"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEX5563431.1"
                     /translation="MSINKFMKGAVTGWMTSHGEHSDIVMSTRIRLARNLRGYRFPIA
                     FTADEARKVDSQISAALLTSDEKGYAYMKMSELPALERQVLVEKHLVSPQLTDVKRHG
                     AVILSEDETLSIMVNEEDHIRIQCIYPGFQLENAYEHADSVDNYLENELPYAFDEDFG
                     YLTSCPSNTGTGMRASVMMHLPALTITKQIERIIPAISRLGMIVRGSYGEGSEAPGNI
                     YQVSNQTTLGKPEKEILSDLKNVALRLIAHERRSREILLSKSRVALENKLFRSLGIIS
                     YARLLPSGEASRCLSDVRLGIDLGIIKDVDMSILNELMIFMQPGFLQQYAGTELTAEE
                     RDIFRADLFRERLRSEQQIDGNNKED"
BASE COUNT         1726 a         2261 c         1662 g         1976 t
ORIGIN      
        1 gcgccatttt cggtttttgt gccatgatcg tgcaatcgac attgcccaac cggtagcctc
       61 tcgactctac aatctcccac actttttgaa ggaggatcgc tgaatccgca tctttgaatg
      121 cttcgtcggt gtccgggaag tgcctgccga tatccccttc cccgattgcg ccgagcgcag
      181 cgtccgttac cgtgtgcaat aggacatccg cgtcggaatg ccccgtcaac cctttttcat
      241 aaggaatcgt aatgccgcct atgatcaatg gccgcccttc ctcaaactga tgcacatcaa
      301 acccttgtcc tatccgaatc attgaaaatc ctcctgccgt ctctgtttca agaggacctc
      361 tccgaacagg aggtcctctt tcgtcgtcat ttttacatta tcatacgtac tttccacaac
      421 tcttaccgga tggccaagcc gttcgacaat catcgattca tctgttccga ggaacccctc
      481 tgcttcggct ttatcagacg cttccttcaa aatttcatac cggaatgctt gcggcgtctg
      541 gatcgcccaa agcttcgatc ggtccaccgt ctcttcgacg atgccgtccg gcgcaacttt
      601 catcgtatcc ttcacttgga cacccgcaat tgcggcaccc tcctccaggg caacttcgac
      661 aagctttcgg acgacatccc tcttgatgaa gggtctcgcc gcgtcatgca caaggacaat
      721 cccaccagat tgatgcgcat tgatgcaagc cgccacgctt tgctgccgct ccgctccgcc
      781 ttcgacgagc gcagccactt tcgtaatgcc gaaacgctca agcatctctt cgatttgtgg
      841 cttttcctca tacttcacgg aaaggatcat tcccgagcaa tccggatccc gttcgaacac
      901 gtccaacgtg tggatgagga tcggcttgcc ggccatctcc aaaaacagct tgttgaaccc
      961 ggcacccatc ctcttgccgc ttcccgctgc cgggagcagt actgtatatg tctccatctt
     1021 cgttcctcat ctttcttctt caatacgcct ttgcggtctt gccgttcttc ggcttcgcga
     1081 aaatcatccg gcccgcggaa gtttgcaaga cgcttgttac gatgacgtcg attgcattgc
     1141 cgatatgcac cctgccatcc tcaatgacga tcatcgtgcc gtcatccaaa taggcgacgc
     1201 cttggttatg ctccttgcca tccttgatga cggcaacatg catctcttca ccggggatga
     1261 cgacaggctt cactgcattt gccagatcat tgatgttcaa taccgcgaca ccgtgcaagt
     1321 ccgccacttt attcaagttg aaatcatttg taacgacctg gccattcatg ctcttcgcga
     1381 gacggacaag tttcaggtcg acttccgcaa catccgcaaa atcttcatcg gtgatcaaaa
     1441 tgctcggccc gtcatccgtc tgcaaccgtt tcaggacgtc aaggccgcgc cgcccttttg
     1501 tccgcttcaa cgaatcggat gaatcggcga tattctgcag ctccgtcaat acgaattgcg
     1561 gcacgacgag aagcccttcc aaaaatccag tcgatgcgat atccgcaatc cttccgtcga
     1621 taataacgct cgtatccaag atcttatggt gcttggcgtc cgactgaccc gcagcctccg
     1681 caccatccct ctttttcgga ctgctgttcc ttgcggcaaa aatggcgtgg gaaaactcct
     1741 cacgtttttt gaagccgacc tggaaaccga ggtatccaag aagaatggaa agcaccaccg
     1801 gcagaactga agtgataaaa gggatttcaa gactgctcag cccgaagctg acaagaaaag
     1861 cgacactcaa tccgaccatc agaccaatcg ttccgaacag caaatcgaaa atcggagcct
     1921 tcaacagacg atcttccatc catttgatga aattcacgat cgggtctgca atgaaaaggc
     1981 tcagcaaata taaaataagt gcacctagga cagcagctac gtaagggttg tcaaccatcg
     2041 gctttgaagt aaaagaagat aaagtgaata aatacggtaa aaataatacg ccgagcgtgc
     2101 ccccgatcag cagcagtccg atctgaacca ctcttttcaa catgtacaac acctccattc
     2161 cttactgata actatacacg ttttccatat ccttcgccgt tttgagccat caaactcaat
     2221 gttttcccaa caagcatagg acaatggaaa agccccggaa gttccttgca tcgcaaaatc
     2281 agggatatgg gacttcctat caaagtccca tgcccttgcc ttttgtcacg aaaatgcgat
     2341 gttcaatgct tcacttatcg tttcgacacc gacaacttga atcccttccg gaaagtccca
     2401 gccgccaata ttggatgacg ggatgatgac cttctcaaag ccgagcttcg ccgcctccgt
     2461 cacacgctgt tcgatacggg agacgcggcg gacttcgccc gtcaagccca cctcgccgat
     2521 gaagcagtcc gtcgctcccg cagccgcatc cctgtagctt gaaacgatgc tcacaaggac
     2581 ggccaagtcg attgccggct cgtcaagctt gacgccgcca gcgactttaa tataggcatc
     2641 ctgcgcttgc agaagcatcc ccatccgctt ttccaggacg gccatcaaca gggagacccg
     2701 attctgatcg atcccggtcg ccatccgctt cggatagttg aagctcgacg gcgtaacaag
     2761 cgcctggatt tcgactagga ttggacgggt cccctccatc gacgcgacga cagtagatcc
     2821 ggcgccgcct tgcgaccttt cccgcaggaa caactcggac ggattcaaca cttccttcaa
     2881 accggattgc agcatttcga agattgcaat ttcattcgtc gagccgaagc ggttcttcac
     2941 actacgcaaa atccgatacg tatgatgacg ctccccttca aaataaagga cagtatccac
     3001 catatgctcg agaaggcgcg gacctgcaat ttgtccgtct ttggtcacat ggccgacgat
     3061 gaaaatagcg atgttttgcg ttttcgcgat gcgcatcagt tcagccgtac attcccgcac
     3121 ttgcgaaaca ctgcccggcg cggacgtcac ttctggatgg tggatcgtct ggatggagtc
     3181 ggcaattaca aacttcggct tcacgtcatt gatcgtctca tggattttcg ccaaatcggt
     3241 ctctgcgtag atgaacagct catccgacgc aacaccgaga cgttcggccc gcagcttcgt
     3301 ctgcctgatt gattcctcac ctgatatgta aaggacgcgt tgcttctgat tcgcgaggag
     3361 tgaagacact tgcaacagca atgtcgattt tcctatgccc ggatcaccgc cgatcaaaac
     3421 aagcgagccc ggcacgattc ccccgccaag cacccggttc aattccccaa gctccgtttt
     3481 aaccctcggt tcctccatcg tttcaattgc actgatcggc atcgctttct gctggatggt
     3541 ttcagtatgc tgaaacgcgc cgcgcggacc tttgcttaca atttcgactt cctcgtccat
     3601 cgtattccat tcaccgcacc cagggcatct ccccatccat tttggggatt cataaccgca
     3661 gtccctacac atgaatttcg ttttccgttt tgccatattt tcctccatat acgaaaagga
     3721 cagccccttc ccgtagatag cgaactctac gggagggcca tcctattatt gtttttctgt
     3781 cactgcaacg cggtctgtct tgacgacgaa ctcgccatcc tctacatcga cgatgacttt
     3841 tccgcccgtc agaaccgttc cgctcaacag ctcctccgat agacggtctt cgacatgttt
     3901 ctgcaaagcc cggcgcagcg gacgtgcacc gtaatccggg tcatatccct cttcagagat
     3961 tttcaattta gcggcatccg tcagctcaag cgtaatatcc tgttccgcaa gacgcttcgt
     4021 caactcgttc gacatgaggg tcacgatttc acgcaagtgc tccttctcaa gcgagtggaa
     4081 gacgatcatt tcatcgacgc ggttcaggaa ctccggacgg aacgcctttt tcaacgcttc
     4141 aagcatcgtg gatttcatat cctcatagtc gcgttcgcca tcttgcaaat tgaagccgac
     4201 gtaacggttc gatttcaacg tttccgctcc gacgttcgac gtcatgatga cgaccgtgtt
     4261 gcgaaaatcg actgtacggc ctttcgaatc agtcaatctt ccatcctcaa gaacttgcag
     4321 gagaatattg aagacgtccg gatgcgcctt ttcgatttca tcgagcaaga cgaccgagta
     4381 cggctttctg cggactttct cagtcagttg accgccttct tcataaccga cataccctgg
     4441 aggcgaaccg acaagccttg acgtagcatg cttctccata tactcggaca tatcgatgcg
     4501 gatcatcgca tcttcatccc cgaacatcgc ttccgcaagc gcccttgcaa gctcggtctt
     4561 cccgacaccc gtaggtccaa ggaaaatgaa tgacccgatc ggacgcttcg gatccttcaa
     4621 tccggctcgg gctctccgga tcgcccttga aatggccgtt acggcttctt tttgcccgat
     4681 gacacgttca tgcagaagct cttccatatt caacaacttg gcggtttccg tctccgcaat
     4741 cttcgcgacc ggaactcccg tccacattgc gacaacttcc gcaatgtcgt tcactgtcac
     4801 ttccgactcc tttttccctt gcttctcttt ccattccgtc ttcgtcgttt cgagctcttc
     4861 tttcattttc tgctctttat cgcggaagga tgccgccttt tcgaattcct ggctttgcac
     4921 cgcagcgttc ttttcgtggc ggatcgcttc cagctttgct tcaagctcct tcaaattcgg
     4981 aggcgtcgta tacgaacgga ggcgcacttt cgatcccgcc tcgtcgatca agtcgattgc
     5041 tttatccggc aggaagcgat ccgagatata tcgatctgac attttcgcag ctgcctcaat
     5101 cgcctcatcc gtaattttca cgcggtgatg cgcttcgtac cggtcacgca agcccttgat
     5161 gatcaagatc gtttcttcga cagtcggttc atcgacttgg atcggctgga aacgtctttc
     5221 aagcgcagcg tctttttcga tatatttacg gtattcatca agcgtcgtgg cgccgatgca
     5281 ttgcaattcc ccacgggcaa gggatggctt cagaatgttg gatgcgtcga tcgcgccttc
     5341 agcgccccca gctccgatca gtgtatgcaa ttcatcaatg aatagaatga tattgcccgc
     5401 ttgacggatt tcttccatca ctttcttcat gcggtcctcg aactctccgc ggtatttcgt
     5461 acccgcaacg accgtaccca tatccagtgt catgacgcgt ttatcacgca ggatttcagg
     5521 cacttcatta ttgacgatct gaagcgcaag cccttccgcg attgcagttt taccgacacc
     5581 cggctccccg atcaatacag ggttgttctt cgtacggcga gctaacactt cgatgacacg
     5641 tgtaatttcc ttgcttcggc cgatgaccgg gtcaagcgtc ccttcgcgtg ccacctctgt
     5701 caaatcgcgg gcaaggctgt caagcgtcgg tgtggaggca gatgatgaag caccttgatt
     5761 gccgacggaa ctgtcattgc ttccaagcaa ttggagcact tgctgccttg cgcggttcaa
     5821 gctcactccc gcattattca atacgcgggc agcaacccct tccccttcac ggatgagcgc
     5881 caacagaata tgctccgtcc cgatatagga atgaccgagc ttacgggatt catcaacgga
     5941 aagctcaatc acctttttcg ctctcggcgt gtaatggacg atcggcccga cttccttcga
     6001 tccggaaccg acaaggcctt ccaccccttt ttcaatcgtg tcgaaactga cgtcgatcgc
     6061 ttccaacgct tttgctgcga tgccgccgcc ttcccgaatg agacccaata aaatatgttc
     6121 agtaccaatg gattcgtgtt tcagtctgat cgcttcttct tgtgcaagct gcaagacttt
     6181 ctgcgcccgc tgtgtaaaac gattgaacat catattcttc cactccttct ctagtaacgt
     6241 ctatcctgtc cccacttgaa atgtgcggat tccttttact ttattcgaat ggatcatgaa
     6301 gcaatccaat tccaacaaat tgaatttgac cattcttgac catctcttgc tttagtataa
     6361 taaaaagaag agtacgaagc aaaactagcg ctcagtcttc tttattattt ccgtcgattt
     6421 gctgctcgga acgcagtcgc tcacggaata gatctgctct gaatatatcc cgttcctccg
     6481 ccgtcaattc cgtccctgca tattgttgca ggaaaccagg ctgcatgaaa atcatcaatt
     6541 cattcaatat ggacatatcc acatccttga tgattccaag gtcgatgcca agtcggacat
     6601 ccgataggca tcgggacgct tcgcctgaag gcagcaatct tgcatacgag atgatgccaa
     6661 gcgaacggaa aagcttgttt tccaatgcga ctcgcgattt ggaaaggagg atttcacgag
     6721 agcgtctctc gtgcgcgata agccgaaggg caacattttt caaatcggaa agaatctctt
     6781 tctccggttt gcccaaagtt gtctgattcg aaacctgata gatattgccc ggtgcttcgc
     6841 ttccctctcc gtagctgccc ctgacgatca tacctaatct tgaaatggct ggaatgatgc
     6901 gctcgatctg cttcgtgatg gtcaatgccg gcaaatgcat catgaccgat gcccgcatgc
     6961 cagtgcccgt atttgacggg cagcttgtca aatagccgaa atcctcgtcg aaggcatatg
     7021 gaagttcatt ctccaaatag ttatcgacag agtcagcatg ctcatacgca ttttccagct
     7081 ggaagcccgg atagatgcat tgaatgcgaa tatggtcctc ttcgttcacc atgatgctca
     7141 gcgtctcgtc ttccgatagg atgaccgctc catggcgctt cacgtccgtc aattgcggac
     7201 tgaccaaatg cttctcgacg agcacttgcc tttcaagcgc cgggagctcc gacatcttca
     7261 tataggcata ccctttttca tcggaagtaa gcaaggcggc cgaaatttgc gaatcgactt
     7321 tccgcgcctc atctgctgta aatgcgatcg ggaatcgata tccccttaaa ttccgggcca
     7381 agcggatcct tgtcgacatg acgatatccg aatgctcccc atgtgacgtc atccaacctg
     7441 tgacggcgcc tttcatgaat ttattgatcg acatcgcttc catcacctcc attgccgcac
     7501 tccgccaaac ggtcttttag caccttcgct tcgtccctaa acgcggcggc atcttcaaaa
     7561 cgctcttcct caatcgcctc ttgcattttc gaccggattt cctcgacttt ctttctcaaa
     7621 gcgaa
//
LOCUS       DASULI010000030         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1457245,
            whole genome shotgun sequence.
ACCESSION   DASULI010000030 DASULI010000000
VERSION     DASULI010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457245"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..420
                     /locus_tag="VFX34_00620"
     CDS             82..420
                     /locus_tag="VFX34_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX5563432.1"
                     /translation="MKMQWSLILSILFAIIIAVFAILNVEPVQVNYLFGKAHLPLILV
                     ILFTALLGAAISGFMAMFRSISANRRINELKKEMTAKEITIATQQNEIAALQHLVEPA
                     INEDKFEGEV"
     gene            533..2881
                     /gene="recJ"
                     /locus_tag="VFX34_00625"
     CDS             533..2881
                     /gene="recJ"
                     /locus_tag="VFX34_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5563433.1"
                     /translation="MIESMKTWTINRPDETIVNAFMKELSIPSIHAKILVSRGFTDVE
                     EAKSFLHMEESCLHDPFLLFDMEKAVSRIRQAIETGEKITVYGDYDADGVTSVTVLTT
                     ALEQLGANVGFAIPNRFEHGYGPNIELFDELHKAGTSLIITVDNGISGTEAIAYAKSV
                     GMDIIVTDHHEIGAELPNADAIIHPRHPEGNYPFGELAGVGVAFKLACALLGEVPEEL
                     YEIAAIGTVADLVPLKDENRFIVKEGIRRMRISSRPAIQALVKVSGTDQHTLSEESLG
                     FMIGPRLNAPGRLGSADPAVHLLKTEDKLEAMSIAEQLDSLNKERQALVTKIADEAEQ
                     EMNDMYGDMLPYVIVIEGEGWNPGVVGIVASRLTEKYYRPSIVLSVDSETGIAKGSGR
                     SIPGFDLFKELSKTADLLPHFGGHEMAAGMSLPMEDVAAFRERLNDQGKLSLTDEMLM
                     PQLKIDVPLEIREIDVDVLESMELLRPFGVSFEKPVYMIENLSASSVRKIGATKNHLK
                     LELSDGVDTLDVIGFGNGGIADEMSPGVKLSVTGDLQVNEWNGRKKPQLLLDDIQSKD
                     RQLFDLRGIREPNRWISVIPEKNNLFIAFQEPSIEYFQPILRNIQIHRFGQVELSETD
                     HVILLDMPSGTEQMKELIEKTNPKRVYAHFFVNESVYFEGIPSREHFGWYYSFLKKRG
                     SFDLRKQVQELSKHRGWKIDTIYFMTKVFSELGFVKIENGLAIIIEDAEKKNLSEAPS
                     YKQRENQIELEKMLVYAPYMELKKWFDEICKETVTREEQVWI"
     gene            2872..3384
                     /locus_tag="VFX34_00630"
     CDS             2872..3384
                     /locus_tag="VFX34_00630"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX5563434.1"
                     /translation="MDLKSFVTIVPDYPKEGISFKDITTIMDNGEAYKYATDQIVEFA
                     RKVGTDIIVGPEARGFIIGCPVAYALEVGFAPVRKPGKLPRETIAVEYDLEYGKDSLT
                     IHKDAIKPGQRVLIVDDLLATGGTVGATVELVEKLGGVVAGCAFLIELSYLNGREKLK
                     GYDIQALITY"
     gene            3512..>4645
                     /locus_tag="VFX34_00635"
     CDS             3512..>4645
                     /locus_tag="VFX34_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX5563435.1"
                     /translation="MAKNRDMTAEDIFEIVASYMNGQHVAFVKKAYEAAKAAHEGQFR
                     SSGEPYILHPIQVAGILAELQMDPSTVAAGFLHDVVEDTDVSREDIISDFGEEVAMLV
                     DGVTKLEKLKFKSNEEKQAENHRKMFIAMARDIRVILIKLADRLHNMRTLKHVPEEKQ
                     RRVAAETLDIFAPLAHRLGISTIKWELEDTALRYLNPQQYYRIVNMMKKKRDEREKYL
                     LNVMDTIRAEIAEMGIEAEINGRPKHLYSIYRKMILQNKEFNEIYDLLAIRIIVKSIK
                     DCYAELGIIHTLWKPMPGRFKDYIAMPKQNLYQSIHTTVVGPAGDPLEVQIRTEEMHR
                     IAEYGVAAHWAYKEGKTVSEKPDNIDSKLKWFREILEFQNESSN"
BASE COUNT         1332 a          943 c         1208 g         1162 t
ORIGIN      
        1 ctacgctgtt cgattggaaa gcgtccgcct gtagcgtaaa tccggtatag tagatgaatc
       61 aaaaaaggaa ggtggaaaag aatgaagatg caatggtcat tgatactcag tattctattc
      121 gccatcatca tagctgtatt cgcaatacta aacgttgaac ctgtccaagt gaattacttg
      181 ttcggcaaag cacatctccc gctcatcctc gtcattcttt tcaccgcgct tcttggcgct
      241 gctatcagcg gcttcatggc catgttcaga tccattagtg ccaatcggcg gatcaatgaa
      301 ttaaaaaagg aaatgaccgc aaaggaaatc acgatcgcta cgcagcaaaa tgaaatagcg
      361 gcattgcaac acctagtgga accagccata aatgaggaca agtttgaagg agaggtataa
      421 gcatcctgcc aaaggatgct ttttttaatc cgtaaatttg gccgcaacct gcatatcccc
      481 tttaagagta tgtcgtcttc cgatataatg ggcttagagg atgtgagcta ttttgataga
      541 atctatgaag acatggacga tcaatcgtcc ggatgaaaca atcgtaaatg cattcatgaa
      601 agagcttagt atcccttcaa tccatgccaa aatccttgtt tctcgcgggt ttacggatgt
      661 ggaggaggct aagtcctttt tacatatgga agaaagttgc ctgcacgatc cattcttgct
      721 attcgatatg gagaaagcgg tcagtcgaat ccgacaggca atcgaaacgg gcgagaagat
      781 taccgtgtat ggagattacg atgcggatgg cgtcacaagc gtcacagtgt tgacgactgc
      841 ccttgaacag ctcggtgcaa acgtcggctt tgccattccg aaccgcttcg aacacggata
      901 tggacccaat atcgaattgt ttgatgagct gcacaaggct ggcacttcgc tgatcatcac
      961 tgtcgataac gggatctcgg gcacggaggc gattgcgtat gccaaatcag tcgggatgga
     1021 cattatcgtg acggatcatc acgagatcgg tgccgagctg ccgaacgctg atgccatcat
     1081 ccatccacgg catccggaag gcaattaccc tttcggcgag cttgcgggag tgggtgtggc
     1141 ctttaaactg gcttgtgctt tactgggcga agtaccggaa gagctgtacg aaatcgcagc
     1201 gatcggtaca gtggcggacc ttgtcccttt gaaagatgaa aacagattca tcgtcaaaga
     1261 agggatcaga cggatgcgga tttccagtcg acctgcaatc caggctctag taaaagtgag
     1321 cggaactgat cagcatacgc tttccgagga atccctcggt ttcatgattg gtccgcggtt
     1381 gaatgctccg ggaagacttg gcagtgccga tcctgctgtc catctgttga aaacggaaga
     1441 taagctggaa gcgatgtcga ttgccgaaca attggattca ttgaataagg aaagacaggc
     1501 gttagtcaca aaaattgcag atgaggctga acaggagatg aatgacatgt atggcgatat
     1561 gctgccgtat gtcatcgtta ttgaagggga aggctggaat ccaggagtag tcggtatcgt
     1621 tgcatcccgt ttgactgaaa aatattaccg gccttcgatt gtcctttccg tggacagtga
     1681 gacaggaatt gcaaaaggtt cagggcgcag tattccgggg tttgatttat tcaaagagct
     1741 ttcgaagact gccgaccttt taccgcattt cggcggtcac gaaatggcgg cgggcatgtc
     1801 gctcccgatg gaggatgttg cggcattccg ggagcgcttg aatgatcaag ggaaattgag
     1861 tttgacggat gaaatgctaa tgccccaatt gaagatcgat gttccactgg aaatccggga
     1921 aatcgatgtc gatgtattgg aatcgatgga attgctgcgg ccatttggtg tgtcgttcga
     1981 aaagccggtt tatatgattg aaaacctttc tgcctcatcc gtcaggaaaa tcggcgcgac
     2041 aaaaaatcat ttgaagcttg agttgtccga cggagtggat acactggacg tgatcggttt
     2101 cggaaatggc gggattgccg acgaaatgag cccgggtgta aagttgtccg tcacggggga
     2161 tttgcaggtg aatgaatgga atggccgtaa gaaaccacag ttgctgctcg atgacatcca
     2221 gtcgaaagat cgtcagcttt tcgatcttag gggtatccgg gagccgaatc gatggatttc
     2281 ggtcattccg gaaaaaaata atttattcat cgctttccag gagccttcaa tcgaatattt
     2341 ccaaccgatc ctgagaaata tccagatcca tcgattcgga caagtggaat tgtcggaaac
     2401 tgatcatgtg atcctattgg atatgccttc cgggacggag cagatgaaag agttgattga
     2461 gaaaacgaat ccgaaaaggg tatatgccca tttcttcgtc aatgaatctg tgtatttcga
     2521 agggattcca agccgggagc atttcggctg gtattacagc ttcctgaaaa agcggggctc
     2581 gtttgatttg cggaagcaag tgcaggagtt atcgaagcac agaggttgga agatcgacac
     2641 aatttatttc atgacaaagg tgttttctga gcttggattt gttaagatag agaatggtct
     2701 tgctataatt atcgaagatg ctgagaagaa gaatctttcc gaggcaccat cttacaaaca
     2761 gagagagaat cagattgaac tggaaaagat gcttgtgtac gcaccatata tggaattgaa
     2821 aaagtggttc gatgaaattt gcaaagaaac cgtcactagg gaggaacaag tatggatttg
     2881 aaaagtttcg tgacaatcgt cccggactat ccgaaagaag gcatcagttt taaagatatt
     2941 acaacaatca tggataatgg cgaagcatat aaatatgcga ccgatcagat tgtcgaattt
     3001 gcaaggaaag tgggaacgga tattatcgtc ggtcccgagg cgaggggatt catcatcgga
     3061 tgtcctgtcg cttatgcact tgaggtcggt ttcgctcctg tccgaaagcc tggaaaatta
     3121 ccgcgcgaaa caatagcggt cgagtatgat cttgagtacg gcaaggattc attgacaatc
     3181 cataaagatg ccatcaagcc gggccaacgt gtattgatcg ttgacgatct gcttgcaaca
     3241 ggcggaactg tcggcgccac agttgaactt gttgaaaaac ttggcggggt agtggctgga
     3301 tgtgcattcc tgatcgagct gtcgtatttg aacggccgtg aaaaactgaa aggctatgat
     3361 attcaagcgc ttatcaccta ttgatgacaa ggccctccgc gatgatgcgg aggatctttt
     3421 tttatccata gggctttaca ttcctgccgg tttatacata caatagaatt atcttttatt
     3481 ttttgcggaa ttagtcgaaa ggagtagaat gatggcgaaa aatcgtgaca tgacggcaga
     3541 agatatattt gaaatcgttg cctcatatat gaatggacag catgtcgcgt tcgtcaaaaa
     3601 ggcgtatgag gcagcgaagg ctgctcatga aggtcaattc agaagttcgg gcgaaccgta
     3661 tattcttcac cctatccagg tggcaggcat tttagcagaa ttgcagatgg atccttccac
     3721 tgtcgcagcg ggcttccttc atgatgtagt cgaagatacc gatgtgagcc gcgaagatat
     3781 tattagtgac ttcggtgaag aggtggcgat gctcgtcgac ggcgtgacga aactggaaaa
     3841 gctgaaattc aaatcgaacg aggaaaaaca ggcagagaac caccgtaaaa tgttcattgc
     3901 gatggcgagg gatatcagag tcattcttat caagctcgcg gaccgtcttc ataatatgcg
     3961 tacattgaaa catgtccctg aggaaaagca acgtagggtt gcggctgaaa ctcttgatat
     4021 tttcgcaccg cttgcacata ggcttggaat ttccacgatc aaatgggaac tggaagacac
     4081 tgctctccgt tatttgaatc ctcagcaata ttaccggatt gtcaatatga tgaagaaaaa
     4141 acgggatgaa cgggaaaaat atttattgaa cgtcatggat acaatacggg cggaaatagc
     4201 cgaaatgggc atcgaagccg agataaacgg cagaccgaag catttgtatt ccatttaccg
     4261 caaaatgatc ctgcaaaaca aggaattcaa tgaaatctac gatctccttg ccatccgcat
     4321 catcgtcaaa agcatcaagg attgttatgc ggagcttgga atcatccata cattatggaa
     4381 accgatgccg ggaaggttca aagattatat cgcgatgccg aaacagaatc tttatcaatc
     4441 tatccatacg acagtagttg ggcctgcggg cgatccactt gaagtccaaa tccggacgga
     4501 agaaatgcat aggattgccg aatacggggt tgcagcgcac tgggcgtata aagaagggaa
     4561 aaccgtctcg gaaaaaccgg ataatattga ttccaaactg aaatggttcc gggagatcct
     4621 tgaattccaa aatgagtctt cgaat
//
LOCUS       DASULI010000031         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1164936,
            whole genome shotgun sequence.
ACCESSION   DASULI010000031 DASULI010000000
VERSION     DASULI010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164936"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFX34_00640"
     CDS             <1..1063
                     /locus_tag="VFX34_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563436.1"
                     /translation="EEELAFTLENLNVDREEMDGRINDVLELTGLTEFRNSVIQQLSG
                     GEKQRIATACALIMEPEVLLLDEPISHLDPYTAKQFISWLDKLQKKRSLTILAIEHRT
                     DLWDGFFDREIQLERMNDADPTYKRAGSKQGRQSLEVIKVSTDSFLKDVTFTLDRGEV
                     AVLAGPNGSGKSTLLKALCRLIPADGTVRPSYLGYVPQSPEFLFVTKTVRDEIAFGGG
                     TAVDELLDRLHLAAVADAHPFAVSHGQKRRVAIAAMLCDGREIVVMDEPTSGQDAASL
                     AELFRLIDERAREGTTFLIVTHDMEFAFHMADSVLLMKDGALTGKFDADQVWRDEELL
                     AAHHLLQPKGWVQHELCFA"
     gene            1044..1799
                     /locus_tag="VFX34_00645"
     CDS             1044..1799
                     /locus_tag="VFX34_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX5563437.1"
                     /translation="MSSALHKMNPSAKFLVVTVCMLTMAFFFNPWTPLLMWVGVMIVQ
                     LLFSRIDWKLWILFMIPFFVTAFGYFWTTLVFAEDKSGPVVLSIWSIKITEVQLNHAL
                     SLSFRVLAFSGLSLLFALTTNPVSFILSLMQQMKLSPKIAYSILVGYQFLPVLKDEFV
                     QIQQAHRLRGAVQEKNPIRRLLHMRLILLPMLAGAVRKAERAAFAMEARGFTGDKRKD
                     FYKVVRVGKVDGYMVSLFMMLLILSCTSVLYID"
BASE COUNT          482 a          432 c          579 g          538 t
ORIGIN      
        1 cgaagaggag cttgcattta cgcttgaaaa cttgaacgtc gatcgggaag aaatggatgg
       61 gcgaatcaat gacgttttgg agttgacggg gctgaccgaa ttccgcaatt ccgttatcca
      121 acagctgtcc ggtggggaaa agcagcggat tgcgactgcg tgtgcattga tcatggagcc
      181 ggaagtcctg ctgctcgacg aaccgatttc ccatttggat ccgtatacag caaaacagtt
      241 catcagctgg ctggacaagc tgcagaagaa acggtctttg acgattttgg caattgagca
      301 tcggactgat ctatgggacg gcttttttga tcgtgaaatc cagttggagc gaatgaatga
      361 tgcggatccc acatataaaa gggccggcag taaacagggg cgacagtcct tggaagtcat
      421 caaagtcagc acggattcat ttctgaaaga cgtaaccttc acgctggacc gcggggaagt
      481 ggcggttctt gcaggcccga acgggagcgg gaaatcgaca ttgctgaaag cattatgccg
      541 attgattccc gcggatggaa ctgtgcgccc ctcatattta gggtatgtcc ctcaatcacc
      601 ggaattttta ttcgtgacga aaacggtgcg ggatgaaatt gcattcggcg gcggaactgc
      661 tgttgacgaa ctgcttgacc gattgcattt ggcggcggtt gcggatgccc atccgtttgc
      721 cgtgagccat gggcaaaaga gaagagtggc aattgccgcg atgctgtgtg acggcaggga
      781 aatcgtcgtg atggatgagc caacatccgg gcaagatgcc gcgtccttgg cggagctgtt
      841 ccgtttgatc gatgagcgtg cgagggaagg gaccaccttc ttgatcgtca cccatgacat
      901 ggagtttgcg tttcatatgg cggattcggt tttattaatg aaggatggtg ctttgacagg
      961 gaaatttgat gctgatcaag tttggcggga cgaggagttg ctcgccgcgc atcatttgct
     1021 tcagccgaaa ggatgggtgc agcatgagct ctgctttgca taagatgaac ccatctgcaa
     1081 agttccttgt cgtcaccgtt tgtatgctga cgatggcatt ctttttcaat ccgtggacgc
     1141 cgttgcttat gtgggtcgga gtgatgatcg tccaactcct cttcagccgc atcgattgga
     1201 aactgtggat cctgttcatg attccgtttt tcgtcacggc gttcggttac ttttggacaa
     1261 cgctcgtttt tgcggaagac aagtcggggc cagtcgtcct gtcgatttgg tcgatcaaga
     1321 ttacggaagt ccagttgaat catgcgttgt cactaagttt tcgtgtcctg gcgttctccg
     1381 gcctgtcatt gctattcgca ctgacgacga atcccgtttc attcatttta agcttaatgc
     1441 aacaaatgaa gctgtcaccg aaaatcgcgt acagcatttt agtcgggtat cagttcctgc
     1501 ccgtgctgaa ggatgagttt gtacaaatcc agcaagccca tcgccttcga ggggcggtgc
     1561 aagagaaaaa tccaatccgg cggctattgc atatgcggct gatccttctc ccgatgctcg
     1621 cgggtgctgt ccggaaagcg gaacgggctg catttgcgat ggaggcaagg gggtttacgg
     1681 gtgacaaaag gaaagacttc tataaagtcg ttcgcgtcgg caaggtggac gggtatatgg
     1741 ttagcctatt catgatgttg ctcattctca gctgtacaag tgttttgtat atcgattaat
     1801 gtcgagggtc ccatttggag tgggaccctt tttccgttca ttcccttatg gtatagtcaa
     1861 tcttatagaa attctgataa tggattcaga aaaggggaaa cgcgttatga tcgatgtaag
     1921 ggaattgaag ttggagaaag gtagacgtgt gattattaca tcagatattc atgcaaactt
     1981 accgctgttt cagcaactgc ttgaaaaggt gaagtacacg agggaagatt a
//
LOCUS       DASULI010000032         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1534586,
            whole genome shotgun sequence.
ACCESSION   DASULI010000032 DASULI010000000
VERSION     DASULI010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534586"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="VFX34_00650"
     CDS             complement(<1..1513)
                     /locus_tag="VFX34_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX5563438.1"
                     /translation="MENRVLKTLEFDKIRDIVATHCTSSAGRTYMEKLVPVSDFQEVV
                     RLLEETDEGLAILRVRGNVPMGGISDIRPHAKRAQIGGVLSAYELMETANTIRASRIL
                     RQFIEAVDEDEDIEIPHFLAKKEALPILTGLEHEINAAIDDNGHVVDSASGTLRSIRQ
                     SLRIQEGRVREKLEGYTRGRNASKMLSDSIVTIRNDRYVIPVKSEYRSHFGGVIHDMS
                     SSGQTLFIEPDGVVQANNEIRNLKMKEKDEIEKILQELSVKVQEVAHDLFVLVDILSE
                     IDIILAKSKYGIANKCTKPEVNNEGYIRLAKARHPLLPIEQAVANTIEFGKDITTIVI
                     TGPNTGGKTVTLKTVGLCTLMAQAGLPIPALDGSEIAVFDSVYADIGDEQSIEQSLST
                     FSSHMVNIVDILKKFDSRSLLIFDELGSGTDPQEGAALAISILDEVHGTGARVMATTH
                     YPELKAYGYNRPGVANASVEFDIDTLSPTYRLLIGVPGRSNAFEISRRLGLNEH"
     gene            complement(1530..3236)
                     /gene="polX"
                     /locus_tag="VFX34_00655"
     CDS             complement(1530..3236)
                     /gene="polX"
                     /locus_tag="VFX34_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX5563439.1"
                     /translation="MNKKTIIRTFEKIALYMELLGENPFKVGAFRKAANVLELDPRSL
                     SEMDDILKLKGIGKGTGAVIIDLMEKGESDLLKELEEEVPKGLIPLLKVPGLGGKRIA
                     KLRESLGIDSIESLRAACESNKVSVIPGFGKKTEEKILHELEQLGTRKGKLPIWQVES
                     IVKAIEEELRGIKEIARFSVAGSFRRAEEKSSDVDFIIVTDEPSAVREKLLEVMPIEE
                     TVAAGDSKLSVVLDMADLVDADFRFVTDEQFATALHHFTGSKDHNVKMRQLAKSKGMK
                     ISEYGVELEDGTVKTFESEADFFAFFDLTFIPPSMRKDGTEIDLAQKIAEIVTFEDIR
                     SDLHMHTTWSDGGYSIREMVEACRDRGYSHMVITDHTQYLRVANGLTPERVRSQIEEI
                     RKLNEEYDDIEIFCGTEMDILPDGTLDFDDDLLQELDFVIASIHSNFNQSQEKIMNRL
                     HAAMKNPYVSMIAHPTGRIIGQRDGYDPDVPQLIEWAKKYDKILELNGNPHRFDLAVE
                     YLEMAQEAGVKIAVNTDAHAIDQLDHMEIGVKYAQKAWLKKETVVNTWPLEQFIREVI
                     RK"
BASE COUNT          803 a          893 c          685 g          973 t
ORIGIN      
        1 tatgctcatt caggccaagc cgcctggaaa tttcaaatgc gttacttctt cccggaaccc
       61 ctatcaacaa acgataggtc gggctcagcg tatcaatatc gaattcaacg ctggcattcg
      121 caacacctgg acggttatag ccatacgcct tcaattccgg atagtgggtc gtcgccatca
      181 ctctcgctcc cgtgccatgc acttcatcca agatggatat ggcaagtgcc gcgccttcct
      241 gcggatcggt tcctgagccg agttcgtcaa aaatgagcag cgatcgggaa tcgaatttct
      301 tcaggatatc gacgatgttc accatatggg aggaaaacgt actcagactt tgctcaatgg
      361 actgttcatc gccaatatct gcatatacgg agtcgaaaac ggcaatttcg gaaccatcca
      421 acgcaggaat cggaagtccg gcctgggcca tcaatgtgca aaggccgaca gttttcaatg
      481 tgacggtctt cccacccgta ttcggtcctg tgatgacaat cgtcgttata tccttgccga
      541 attcaatcgt gttggcaact gcctgctcaa tcggcaacaa tgggtggcgc gctttcgcca
      601 gacgaatata cccttcatta ttcacttcag gctttgtgca tttattagcg atcccatatt
      661 tcgattttgc aagtatgata tcgatttcag ataaaatatc gacaaggaca aaaagatcat
      721 gggccacttc ttggactttc acggatagct cctgcaatat cttttcgatt tcgtcctttt
      781 ccttcatctt caaattgcgg atttcattat tcgcctgcac gacaccatcc ggctcaatga
      841 acaacgtttg cccggatgat gacatgtcat ggatgacccc gccgaaatgg gatctatatt
      901 ccgacttaac gggaatgacg tatctgtcgt tccggattgt cacaatggag tcggaaagca
      961 ttttcgatgc attccttccg cgcgtatagc cctcgagctt ttcccggaca cggccttcct
     1021 ggatgcgcaa gctttgacgg attgaacgta atgtgcctga agcactgtcg acaacatgtc
     1081 cattgtcgtc gatggctgca tttatttcat gctcaagtcc tgtaagaatc ggcaaagctt
     1141 ccttcttggc tagaaaatgc gggatttcaa tatcttcatc ttcatcgacc gcttcgatga
     1201 actgtcttag aatcctgctg gcgcggatcg tattggcagt ttccatcaat tcatatgcac
     1261 tcagcactcc gccgatttgt gcgcgttttg catgggggcg tatgtcgctg atgccgccca
     1321 tcggtacgtt gccccggacc cttaaaatgg caagcccttc atccgtttct tccagcaatc
     1381 ggacaacttc ctgaaagtcg cttaccggaa ctagtttttc catgtatgtc cgtcctgcgg
     1441 atgacgtgca atgagtcgcc acgatatccc ggatcttatc aaattcaagt gtttttaaga
     1501 cacggttttc caatgcctta cacttccttt catttccgga tgacttcccg gatgaattgt
     1561 tcaagcggcc acgtattgac gaccgtctct tttttcaacc acgctttttg tgcgtacttc
     1621 acgccaattt ccatatgatc caattgatcg attgcatgcg catcggtatt gacggcaatt
     1681 ttcacgccag cttcctgcgc catttccaag tattcgaccg ccaagtcgaa acgatgcgga
     1741 ttgccgttca gttcaaggat tttatcgtac tttttcgccc attcgatcaa ttgcggcaca
     1801 tcaggatcat agccgtccct ctgaccgatg atccggccag tcggatgggc aatcatcgaa
     1861 acatatggat ttttcattgc cgcatgcagc ctattcatga tcttctcctg tgactggtta
     1921 aaatttgaat ggatggatgc aatgacaaaa tccagctcct gtaataaatc atcatcaaaa
     1981 tcgagagtgc catctggcag gatatccatc tccgtcccgc agaaaatctc aatatcgtca
     2041 tattcctcat tcaatttcct gatttcttca atttggcttc ggacccgttc tggcgtcaat
     2101 ccattcgcca ctctcaagta ttgcgtatga tcggtgatga ccatatgcga atatccccga
     2161 tccctgcatg cttcgaccat ttcccgaatt gaatagccgc cgtcagacca agtcgtatgc
     2221 atatgaaggt cgctgcggat atcttcgaac gtaacaattt ctgcgatttt ttgagcaagg
     2281 tcaatttccg ttccatcctt acgcatagaa ggcgggatga aagtcaaatc gaagaaagcg
     2341 aaaaaatcgg cttccgattc gaacgtcttg accgttccat cttccaattc aacgccgtat
     2401 tcgcttattt tcataccttt cgatttggca agctgacgca tcttcacatt atggtctttc
     2461 gaacctgtga aatggtgcaa tgcagtcgcg aattgttcat ctgtcacaaa acggaaatct
     2521 gcatctacca aatcggccat atcgagtacg accgacagct tggaatcccc tgccgcaaca
     2581 gtttcctcaa tcggcatcac ttctaacagc ttttcccgaa ctgccgaagg ttcatccgta
     2641 acaatgataa agtcgacatc gctgcttttt tcctctgccc ggcggaaact tcccgctact
     2701 gaaaaacggg cgatttcttt tattcctcgc aattcctctt caattgcttt gacaatcgat
     2761 tccacctgcc aaatgggcag cttccctttt cgtgtgccaa gctgttcgag ttcatgcaat
     2821 attttctctt cggtcttttt tccaaaccct ggaatgacgc tcactttatt cgattcacaa
     2881 gcagctcgca gtgattcgat ggaatcgatc ccaagtgatt ctctaagctt ggcaattctt
     2941 ttaccgccaa gtccaggtac tttcagcaaa gggatgagcc ctttcggaac ttcttcttca
     3001 agctccttta ataagtcgga ctcccctttt tccatcaaat cgatgatgac cgcgccggtc
     3061 cctttgccga ttcctttcaa ttttaaaata tcatccatct cggaaaggct tcgcggatcc
     3121 agttcgagca cattcgccgc tttccggaac gctcctacct taaatggatt ctcccctaac
     3181 aattccatat ataacgcgat tttttcaaat gtgcgaatga ttgttttctt gttcatggct
     3241 aaccactcct tcatttaaat gaatcattct tgcaaaaaaa gcttccctcc aaccaggggg
     3301 gaagccgtac agatcgtccg tatggaacag cctgcgttat tgtacgggct gcaa
//
LOCUS       DASULI010000033         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_308567, whole
            genome shotgun sequence.
ACCESSION   DASULI010000033 DASULI010000000
VERSION     DASULI010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308567"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFX34_00660"
     CDS             <1..274
                     /locus_tag="VFX34_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5563440.1"
                     /translation="TTWQRLLKVQIPLAQPTIMAGVNQSIMLSLSMVVIASMVGAPGL
                     GEEVYRAVTQLKTGVGFETGLSIVIVAIILDRITQHAGKKKQGGTI"
     gene            274..1179
                     /locus_tag="VFX34_00665"
     CDS             274..1179
                     /locus_tag="VFX34_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5563441.1"
                     /translation="MNLTKKMFGLGAAALLAVGLAACGSDDKADGSSGDGSSSVGESV
                     DYKITGIDPGAGIMEATDRAIKDYELDKWDVTTGSGAAMTAALKKAYDAEKPIIVTGW
                     TPHWKFAKFDLKYLEDPKGSFGGEEQIRTIGRTGLAEDLPEAHQILSNFHWSEEDMGE
                     VMVAIQEGEKEEVAAQNWIDANADKVAEWTDGVDKVDGDAIKLAYVAWDSEIASHNVM
                     KLVLEEMGYKVTLTQVEAGPLWTAIADGSADASLAAWLPVTHETYANKFEGKFEELGI
                     NMEGVKIGLVVPQYMDIDSIEDLKE"
     gene            complement(1220..1627)
                     /locus_tag="VFX34_00670"
     CDS             complement(1220..1627)
                     /locus_tag="VFX34_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563442.1"
                     /translation="MYGNRFLHDHRTVHALRWAKSSNGLSEIITTHGPCLSHLSPMEL
                     LKQLPKAHQAILQTEPPDHIDCVWILNRSFKTLEMEEYLTDVIFDDGTSLLIPVRKKF
                     IDDKCNFLHTFAYHGRKLNYIFPCIKEFNQHYY"
     gene            1876..2253
                     /locus_tag="VFX34_00675"
     CDS             1876..2253
                     /locus_tag="VFX34_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5563443.1"
                     /translation="MKTITEVAESFGVSTRTIRYYEEIGLLAPDRSEGNQRLFSNAEL
                     AKLKLIFRGKRYGFSLEDIKEMVLLFDKDRTGRKQLEVTIEYGKRRIGEIDRKILELQ
                     EMKAEMEELLEEFTEKLDNSKGE"
     gene            2258..>2782
                     /locus_tag="VFX34_00680"
     CDS             2258..>2782
                     /locus_tag="VFX34_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5563444.1"
                     /translation="MNSSQLLERNARKYPETEAVVSMGKRTTYRELDRRVNKFGHALL
                     NEGIRKGSKVALFLPNVEEFIVAYFAVQRIGAIVVPINVKLATEELTYVLNHSDAEAL
                     IAHEMLFETVKSLQSPSLLIKTGEASGNWKAFETLIQNADDKPIPCNLKEDDESTILY
                     TSGTTGNPKGVLFSY"
BASE COUNT          811 a          583 c          764 g          624 t
ORIGIN      
        1 gacgacgtgg cagcggctgt tgaaagtgca aataccactc gcccaaccga ccatcatggc
       61 aggggtcaac caaagcatca tgctttccct ctccatggtc gtcatcgcgt cgatggtagg
      121 cgcgccggga ctaggcgagg aagtgtacag ggcggtgacc cagttgaaga cgggcgtagg
      181 ctttgaaaca ggactatcga tcgtcatcgt cgccatcatc ctcgaccgga ttacacagca
      241 cgcaggcaag aaaaaacaag ggggaactat ttaatgaatt tgacaaagaa aatgtttgga
      301 ctaggagcag cagcactact cgcagtaggc ttggcggctt gcggaagcga cgataaagct
      361 gacggatcaa gcggagacgg ctcttcgtcc gtcggcgaat ccgtcgatta taaaatcaca
      421 gggatcgacc cgggcgcagg catcatggaa gcgaccgaca gggcgatcaa agattatgag
      481 cttgataaat gggacgtcac tacgggatcg ggcgctgcaa tgacagcggc tttgaaaaaa
      541 gcgtatgatg ccgaaaagcc gatcatcgtc acaggttgga cgccgcactg gaaattcgcg
      601 aagttcgact tgaaatactt ggaagaccct aaaggttcat tcggagggga agagcagatc
      661 cgtacaatcg gaagaaccgg acttgcggaa gaccttccgg aagcccacca gatcctatcg
      721 aacttccatt ggtctgaaga ggatatgggt gaagtgatgg ttgccatcca ggaaggcgaa
      781 aaagaggaag tcgcagcaca aaactggatt gacgcgaacg ctgacaaagt cgctgaatgg
      841 acagatggcg tggacaaagt ggacggcgat gcgatcaagc ttgcatacgt cgcttgggat
      901 agtgaaatcg cgagccataa cgtcatgaaa ctcgttcttg aagagatggg ctacaaggtt
      961 acattgacgc aagtcgaggc aggaccgctt tggacagcga ttgctgacgg aagcgcggat
     1021 gcatcactcg cggcatggtt gccggtgacg catgaaacgt atgcgaataa attcgaaggc
     1081 aaattcgaag agctcggcat caatatggaa ggcgtgaaaa tcggcctcgt cgtaccgcag
     1141 tatatggaca tcgattcaat tgaagatttg aaggaataac agatcaggca gcccgctact
     1201 atagtgggct gcctgttttt cagtaataat gttggttgaa ttctttgatg caaggaaata
     1261 tgtagttcag ttttcttccg tggtacgcga atgtatgtaa aaaattacat ttatcatcaa
     1321 taaacttctt gcggacagga atcaatagac ttgtcccatc atcgaaaatg acgtcggtca
     1381 gatactcctc catttcaagc gttttaaagg aacgattcaa gatccataca caatcgatgt
     1441 ggtcaggcgg ttccgtttgc aagatcgcct gatgtgcctt gggcagttgc tttagcaatt
     1501 ccatagggct tagatgggaa agacagggac catgcgtcgt gataatctcc gacaagccat
     1561 tggatgattt cgcccaacgc aaggcgtgaa cggtccgatg gtcatgcaaa aaacgattac
     1621 catacataat ttatcctcct ttactcatgt aatgaattta ttatagcacg atagattgtc
     1681 gaaatatagt caaaatgtat caaaaaattt aaatctttta aaaatacttt aaaccgtaca
     1741 tattgcaggg cttttttcgg agtcccccat acttgaacaa cttcggacta gttgattcat
     1801 tattctaaaa aaacacttga cctttacgtc aacgtcaatg ctatagtcga gggaaaggct
     1861 gaaggggtgg tgaagctgaa gacgattacg gaagtggcgg aatcatttgg ggtgtcgacg
     1921 cgtacaatcc ggtactacga ggaaatcggt ttgctggcgc cggatcggtc ggaggggaat
     1981 caacggcttt tttcgaatgc cgagcttgcg aagctgaagc tcattttccg cggcaagcgt
     2041 tacgggtttt cgttggagga catcaaggag atggtgctgc tgttcgacaa ggaccggacg
     2101 gggcgcaaac agcttgaggt gacgattgaa tacggcaaac ggcgtatcgg ggaaatcgat
     2161 cggaaaatcc tggagctgca ggaaatgaag gccgaaatgg aagaactgct cgaagagttc
     2221 actgaaaaac tggataattc gaaaggggaa tgaagggatg aacagttcac agctgttgga
     2281 gaggaatgca aggaaatacc cggaaacaga agcggtcgtc agcatgggga agcggacgac
     2341 gtatagggaa ttggacaggc gcgtcaataa gttcggtcat gcgctgctga atgaaggtat
     2401 ccgaaaaggt tcgaaagtgg cgttgttttt gccgaatgtc gaggaattca tcgtcgccta
     2461 tttcgccgtt cagcggatcg gtgcaatcgt cgttccgatc aacgtcaagc tggcgacgga
     2521 agaattgaca tatgtgctca atcattcgga cgctgaagcg ctcattgcgc atgaaatgct
     2581 atttgaaacg gtgaaatcat tacaatctcc ttctctatta atcaaaacag gggaagcgtc
     2641 cggaaactgg aaagccttcg aaacgcttat tcaaaatgca gacgataaac caattccgtg
     2701 caatttaaag gaagacgacg aatcgacgat cctttacacg tcaggaacga caggaaatcc
     2761 gaaaggggtg ctgttcagct at
//
LOCUS       DASULI010000034         8074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1359068,
            whole genome shotgun sequence.
ACCESSION   DASULI010000034 DASULI010000000
VERSION     DASULI010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359068"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1230)
                     /gene="recF"
                     /locus_tag="VFX34_00685"
     CDS             complement(109..1230)
                     /gene="recF"
                     /locus_tag="VFX34_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX5563445.1"
                     /translation="MFIERLALTDYRNYASLELSFSPHINVLIGENAQGKTNIMEAIY
                     VLSMAKSHRTANDRELIRWEQEYGKIEGDIKRKYGRLPLELTISKKGKKARVNHLEQN
                     RLSLYIGQLNVVMFAPEDLNLVKGSPQVRRRFLDMEIGQISPVYLHDLLTFQKVLKQR
                     NAILKDNRGKLDFTDVMFDIYTEQYIQLAVQIIRKRFYFIELLQKWAEPIHKGISRGL
                     EQLKVTYGTLKELDSGQTAEQMEEILSRKLHEVRRRELERGMTLIGPHRDDLHFFVNG
                     YDIQTYGSQGQQRTTALSLKLAEIDLVKQEVGETPVLLLDDVLSELDDYRQSHLLNIM
                     QGQVQTFVTTTNVAGLDHETIREAELYEVSAGKVSDKGN"
     gene            complement(1220..1459)
                     /gene="yaaA"
                     /locus_tag="VFX34_00690"
     CDS             complement(1220..1459)
                     /gene="yaaA"
                     /locus_tag="VFX34_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEX5563446.1"
                     /translation="MELMKIDTEFITLGQLLKMSNCISSGGMAKWYLEENTIYVNGEV
                     DRRRGKKLYDGDIVTIPGAGKFKISSSLDGRSDVH"
     gene            complement(1621..2754)
                     /gene="dnaN"
                     /locus_tag="VFX34_00695"
     CDS             complement(1621..2754)
                     /gene="dnaN"
                     /locus_tag="VFX34_00695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5563447.1"
                     /translation="MKFEIMRDWLLEGLNDVMKAISQKVANPILTGVKMDVSEKGLTM
                     TGSDSDITICTFIPVEQDGEQIIRVIESGSIILQAKVFSEIVRKLPTNDVTIEVENMQ
                     THIQSGKSEFHLIGSNSDEYPVLPNVKDEYRFSLPSDLMKSIIKETVFAVSQQETRPI
                     LTGVHWETDEEKLVCVATDSHRLARREIVPEMMPESPFSVVIPGKSLQELNKILEDTS
                     DVVEIVIADQQVLFKSRNVLFYSRLLEGNYPDTSRLIPSEYKTTVQVNGRKLLQAIDR
                     ASLLAREDRNNIVRFAAEGGNVIEVSSNSPEIGKVEELIEAIDVNGEDLKISFSAKYM
                     MDALKAIEGQDIAIHFTGAMRPFILKSMESDAILQLILPVRTF"
     gene            complement(2926..4275)
                     /gene="dnaA"
                     /locus_tag="VFX34_00700"
     CDS             complement(2926..4275)
                     /gene="dnaA"
                     /locus_tag="VFX34_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5563448.1"
                     /translation="MDQLEKLWNDVLSKIEDKISRPSFETWLKSTKLMSYGDENVTIA
                     VPNSFAKDWLENHYVHLITGILSELTGEDRLIHFVVPKEMEETDFQVPKPAVKQTDRS
                     AIQSASIMLNPKYTFDTFVIGSGNRFAHAASLAVAEAPAKSYNPLFIYGGVGLGKTHL
                     MHAIGHYILEQDPSKKVVYLSSEKFTNEFINSIMNNKAGDFRNQYRNVDVLLIDDIQF
                     IAGKEQTQEEFFHTFNTLHEESKQIIISSDRPPKEIPTLEDRLRSRFEWGLITDIAPP
                     DLETRIAILRKKAKADGLIDISDEVMLYIANQIDTNIRELEGALIRVVAYSSLVNMDI
                     TTELAAEALRDIIPNSRPRTVSILDIQKTVGEHYNIRLEDFTAKKRTRAIAFPRQIAM
                     YLSRELTDSSLPKIGSEFGGRDHSTVIHAHEKISKQLKDDQNLQQDIQDIKGILGRG"
     gene            4792..4926
                     /gene="rpmH"
                     /locus_tag="VFX34_00705"
     CDS             4792..4926
                     /gene="rpmH"
                     /locus_tag="VFX34_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5563449.1"
                     /translation="MKRTFQPNKRKRSKVHGFRARMSSKSGRKILAARRRKGRKVLSA
                     "
     gene            5244..5582
                     /gene="rnpA"
                     /locus_tag="VFX34_00710"
     CDS             5244..5582
                     /gene="rnpA"
                     /locus_tag="VFX34_00710"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003351571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5563450.1"
                     /translation="MKKRQRVKKNEDFQKVFKKGKSVANRQFVVYSYRKEGQQEFRIG
                     LSVGKKLGNAVTRNRIKRYIRQAFLEMKEELKNDRDYVIIARHQAATLDFHETKKSLQ
                     HVLRIAKAFN"
     gene            5767..6537
                     /gene="yidC"
                     /locus_tag="VFX34_00715"
     CDS             5767..6537
                     /gene="yidC"
                     /locus_tag="VFX34_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX5563451.1"
                     /translation="MKKRAALLLLLTVLSVFLAGCSEFNQPIYESSEGFWNKYIVWPI
                     VSLIVTFKDLLGTYGLGIIAVTIIIRLVLLPLMIKQAQNSKRMQEMQPILAGLKEKYK
                     SKDAVTQQKYREEMQRVMTEQKINPAAGCLPVLIQMPIIIGLYHAISRMNATPEIEIG
                     KFLWFELATPSITLAIVAGLMQFVVLRTGPAMDNPQMKVMMYIMPFMIMIFGTFLPAA
                     LALYWVIGNIISIIQNIFIYKPFKKKEVAPVKVGGKRK"
     gene            6534..7313
                     /gene="jag"
                     /locus_tag="VFX34_00720"
     CDS             6534..7313
                     /gene="jag"
                     /locus_tag="VFX34_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX5563452.1"
                     /translation="MKKITRKGATVDAAVEAALRELNVTRNEVDVEIVDAGKKGFLGF
                     GAREAEVTITVKETVKQEAIEPEEKIETIQEETMESAIEEAPVVVPMEPVTQTIEVEA
                     MEPIASDEQAIDETKAYITEIAKAMGIDDLVVKHEMDGKYLTFQMESEKAAFLIGKRG
                     QTLNALQQLAQLVANKTSEQFKVVRLDVGDYRERREQSLEQFAERMADQAVRNGRKVQ
                     LEPMPSYERKVIHNALSNRLDIETYSEGTDPNRYLVIEPIS"
     gene            7490..>8074
                     /locus_tag="VFX34_00725"
     CDS             7490..>8074
                     /locus_tag="VFX34_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX5563453.1"
                     /translation="MHFDTIAAISTPMGEGAIAIVRLSGDEAIGIADKIFRSPSGKKL
                     ADEQSHTIHYGHLADPSTEEIVEEVMVSIMKAPKTFTREDVVEVNCHGGVVAVNRVLN
                     LVLKEGARLAEPGEFSKRAFLNGRIDLSQAEAVMDLIRAKTDRAMNVALNQMEGKLSK
                     LIGELRQALLETLAQVEVNIDYPEYDDVEEVTIPL"
BASE COUNT         2267 a         1753 c         1825 g         2229 t
ORIGIN      
        1 tcgcatcata agctgtctgc aattcttttt cttccatagc caaaatgtcc acctttcctt
       61 tccgaacatc aatctaaacc ttctatcact ttcaaacagg aacggacttc aattcccttt
      121 atcgctaacc ttgcctgctg atacttcata caactccgct tcacgtatcg tctcgtggtc
      181 gagtcccgca acattcgtcg tcgtgacgaa tgtctgcacc tgtccttgca tgatgttcag
      241 caggtgggac tggcggtaat catcgagctc ggaaagcaca tcatccaaaa gaagaaccgg
      301 agtttctccg acttcctgtt tgacaagatc tatttccgca agtttaaggg acagcgccgt
      361 tgtccgctgc tgcccttgcg aaccgtacgt ttggatatca tagccattca caaaaaaatg
      421 gagatcgtca cggtgcggtc caatcagcgt cattccgcgt tccaactcac gacgtctcac
      481 ttcgtgcaat tttcggctga gtatttcttc catctgttcg gcagtctgcc cagaatccaa
      541 ctcttttaat gtcccatacg tcacttttag ctgctcaaga cctctggaaa tccctttatg
      601 gatcggttct gcccattttt ggagcaattc gatgaaataa aaccgtttcc ggatgatctg
      661 cacagctaat tgtatatatt gttctgtata tatatcgaac atgacgtccg taaaatccaa
      721 tttaccgcga ttgtctttga ggattgcgtt ccgttgcttt aagacctttt gaaaagtcag
      781 caggtcatgg agatagacgg gggagatttg tccgatttcc atgtctaaga atcttcttcg
      841 gacctgtgga cttcccttta caagattcaa gtcttcaggg gcaaacatga caacgttgag
      901 ctgtccgata tacaggctga ggcggttttg ctccaaatgg ttgacccgcg ctttcttgcc
      961 ttttttcgat atcgtcagtt ccagcggaag acgtccgtat ttccgcttta tgtccccttc
     1021 tattttacca tactcttgtt cccaacgtat aagttcacga tcatttgcgg tgcgatgcga
     1081 ttttgccatg gataatacgt agatcgcctc catgatgttc gtctttcctt gtgcattttc
     1141 accgatcaga acgttgatat gcggagaaaa ggagagttcc agggaagcgt aattccggta
     1201 atccgttaaa gccagccgtt caatgaacat ccgatcgacc gtctagggat gaggaaattt
     1261 tgaatttgcc tgctccggga atggtgacga tatctccgtc atatagtttt ttgccccgtc
     1321 tgcgatcgac ttcgccattt acgtatatgg tattttcctc caaataccat ttagccatgc
     1381 cgccggagct aatgcaattc gacattttca gcagttgacc taatgtgatg aattccgtat
     1441 cgattttcat gagttccatc cgcttcaatc cttcataaag tagtctgtcc ttctatttta
     1501 ccgaactttc agctgtaagt aaaaatgtat gtgaaaagga cgtgcaataa ataaaaagaa
     1561 acaggcgaag gaaaacgttt tgcattcctt gcctgctgtg ttatgccagt ttcttgattt
     1621 ttaaaatgtc ctgacgggta agatcaattg taaaattgcg tcgctttcca ttgatttcaa
     1681 gatgaatggc ctcatcgcac ctgtgaaatg gatggcgatg tcttgccctt cgatcgcttt
     1741 caatgcatcc atcatgtatt ttgcactgaa ggaaatcttt agatcttcac cattcacatc
     1801 gattgcctcg atcaattctt caacctttcc gatttccgga gagtttgagg aaacttcgat
     1861 gacattgccg ccttctgcag cgaatcggac aatgttattt cggtcctcac gtgcaagaag
     1921 tgaagcccga tcaatggctt gcaataattt acgaccattc acttgtacag tcgttttata
     1981 ttcggaaggg atcaatcttg atgtatccgg ataattccct tctaaaagtc ttgaataaaa
     2041 cagtacattc ctcgatttga acaagacttg ctgatctgcg ataacgattt cgacaacgtc
     2101 tgaggtatcc tcaaggattt tattcagctc ctgtaggctc ttccctggga taacgacgct
     2161 gaatgggctt tcaggcatca tttcaggaac gatttccctt ctggcgagac ggtggctgtc
     2221 tgttgctaca caaacaagtt tctcttcgtc tgtttcccaa tgaacacctg tcaaaatcgg
     2281 gcgtgtttct tgctgggaaa cggcaaatac ggtctctttg atgatcgatt tcatcaaatc
     2341 agacggcaat gagaagcgat attcgtcttt tacatttggc agaacagggt attcatctga
     2401 attcgaaccg atcagatgga attcggattt tccggattga atatgggtct gcatgttttc
     2461 aacttcgatc gttacatcat tcgtcggtaa tttccgtacg atttcactga agactttcgc
     2521 ctgaagaatg atcgatcctg attcaatgac acggatgatc tgctcgccat cttgttcgac
     2581 tggaatgaat gtgcagatcg taatatcgga atcgcttcct gtcatcgtca atcctttttc
     2641 actcacatcc attttcacgc cggtcaaaat ggggttggcc actttctgac tgattgcttt
     2701 catgacatca ttcaatcctt cgagtaacca atctctcatt atttcaaatt tcattttgca
     2761 ccctcgctta tataataatt agttaagtaa taaagatagt aatagtaata ggggctgtgg
     2821 aaatgtggat aagccccgaa aacctatatg ccggaaaaca attcacatgt gcataaaatg
     2881 tgcacagctt gttccggata tagaaggtta ttcacatgtg gattttcatc cccggccgag
     2941 aatgccttta atatcctgaa tatcctgttg tagattctga tcatctttca gctgctttga
     3001 aatcttttca tgtgcatgga tgacggtcga atgatcacgg ccgccaaatt cggatcctat
     3061 tttcggcaat gaggaatccg taagttcacg tgaaagatac atagcgattt gacgagggaa
     3121 agcgatcgcc ctcgttcttt ttttggccgt gaaatcttcc agccgaatat tgtaatgctc
     3181 cccgaccgtt ttttggatat ccaatatgct gactgtgcgc ggtctggaat ttggaatgat
     3241 atccctcaaa gcttccgctg ccagttcagt cgttatgtcc atattgacaa gcgaggaata
     3301 ggcaacaacg cgaatcaaag ccccttccaa ttcacggatg ttcgtgtcga tctgattggc
     3361 gatatacagc atgacttcat cagatatgtc aatcaatcca tccgctttcg ccttcttgcg
     3421 taagatggca atccgcgtct cgagatccgg aggcgcgata tctgttatca aaccccattc
     3481 aaaacgggat ctgagacgat cttccaacgt cggtatttcc ttaggaggac ggtcacttga
     3541 aatgatgatc tgcttcgatt cctcatgcag cgtattgaac gtatggaaaa attcctcttg
     3601 cgtctgttct tttcctgcaa tgaattgaat atcgtcaatt agcaggacat ctacgttccg
     3661 atactgattt ctaaaatcgc ccgctttgtt attcatgatc gaattgatga attcattcgt
     3721 aaacttctcc gatgataaat aaacgacctt ttttgacggg tcttgttcca aaatataatg
     3781 cccgatagcg tgcatgagat gggtttttcc gagtcctacg cctccataga tgaacaacgg
     3841 gttatatgat ttagccggcg cttcggctac agcaagcgat gctgcgtggg caaatctgtt
     3901 gcccgagccg atgacgaatg tatcgaatgt atattttggg ttcaacatga tggatgctga
     3961 ttggatggct gatctgtcag tctgtttcac tgctggtttc gggacttgga aatctgtttc
     4021 ttccatttct ttcggaacga cgaaatgaat gagtcgatct tctccagtca attccgagag
     4081 gatgcctgta atcaaatgca cgtaatgatt ctcaagccaa tccttcgcaa acgaatttgg
     4141 aacagcaatt gtaacgtttt catccccata ggacatcagt tttgtcgatt tgagccaagt
     4201 ttcaaaactg ggtcttgaaa ttttgtcttc aattttcgac aaaacatcgt tccataattt
     4261 ttcaagctga tccactcgtg ggcctccttt acatagaaaa taaaaaaaca caggaatatg
     4321 tattatacac agaatccgct cctgtggata agaaatatcc aaaagtgttg aaaaaattat
     4381 ccacaaccta tcaacaattg tggataaaaa aattagattt ttaatttgtt aacagcaaaa
     4441 tcctctatat tgtatcaaga aactataaga ttgacaaggg attctattag ttattaacaa
     4501 tagcggcaac tgtggagaat tactttatcc acagcctttt gtatgtgcaa aaggtgtaga
     4561 taagacctgt gcataaaaaa acggcgaaaa atttctatga acatatttgt cgactgtcgg
     4621 tttttgtaat tgtggataac ctattttgtc gaatgatgcg gttgaaattt gtaaaacgga
     4681 agtagttagt tgaaattgca tacagaaaaa acggttgaca tcttcgtcca tgccctttat
     4741 actgttcaag actgtcagat tgtaaaggga caatgcagga ggtgtcttta aatgaaacgt
     4801 acattccaac caaataaacg taaacgtagt aaagttcacg gcttccgtgc aagaatgagc
     4861 tcaaaaagcg gtcgtaaaat tctcgctgct cgtcgtcgca aaggaagaaa agtgctttca
     4921 gcataagacc actgtaccca gtggtctttt ttttataaca aaagcgaagg cggcctgcct
     4981 agcagaggtt cgcagtctaa aggcgcgaca tcctgtcgca acgactgcat gaccggcttc
     5041 cttccggcct caggcataag gcggacatgc gaaacggcgc tttttgccgt gcagcagggc
     5101 tgacttatga ccccgagcaa gtcgaactgc gaaggggtcg atttgcctta atttctgtgg
     5161 tctttgcaga aattaaggca tcgatctggc cgccggagct ggatgataac cagataccat
     5221 gacgatctat ggaggtgaac tgtttgaaga aacgtcagcg ggtcaaaaag aacgaagact
     5281 ttcaaaaagt atttaaaaaa ggcaagtccg tagcaaatcg gcaatttgtc gtctattcct
     5341 ataggaagga aggacagcag gaattcagaa tcggcttatc cgtcggcaag aaattaggga
     5401 atgcggtgac gcgcaaccgt attaaacggt acatccggca agccttcctg gaaatgaaag
     5461 aagaactgaa aaacgaccgg gactatgtta ttatcgccag acaccaggcg gccacacttg
     5521 atttccacga aacaaaaaaa agcttgcagc acgtattgag gattgccaaa gccttcaact
     5581 gatttctcta ttgatcctcc ggaggatgtc acagattttt aggagagctt ttcgagcaaa
     5641 ctcgaaaaaa actggacgca ataacgccga gacattattg atttagaacc agaagtagaa
     5701 cgtaataaac gttcatgata aactgaaatc atcgatagtt ttagaagcag atatggggga
     5761 aacgacttga agaaaagagc cgctttatta cttttgctga ccgtactgtc agtattcctt
     5821 gccggatgtt cggaattcaa tcaaccgata tatgaatcga gcgaaggttt ttggaataaa
     5881 tacatcgtct ggccgattgt gtcgttgatc gtcacgttca aagacctgtt aggcacgtat
     5941 ggattaggaa tcatcgccgt cacgatcatc atccgactcg tattgcttcc attaatgatc
     6001 aagcaagcac agaattcgaa gcggatgcag gaaatgcagc cgatccttgc cggattgaaa
     6061 gagaaataca aatcgaagga tgctgtaacg cagcaaaagt accgtgaaga aatgcaacga
     6121 gtgatgacgg agcagaaaat caatcctgcc gcaggatgtc tgccagtatt gattcaaatg
     6181 ccgattatca tcggactata tcatgccatc agcaggatga atgccactcc ggaaatcgaa
     6241 atcgggaagt tcctttggtt cgaactggca accccgagca ttacgcttgc cattgtcgcg
     6301 gggctcatgc agtttgtcgt cttgcggaca ggcccggcaa tggataatcc gcaaatgaaa
     6361 gtgatgatgt atatcatgcc tttcatgatc atgattttcg gtacattcct gccggcagca
     6421 cttgcacttt attgggttat cggtaatatc atttcaatta tccagaatat cttcatttac
     6481 aaaccattta agaaaaaaga agtggcacct gtaaaagtgg gagggaaacg gaaatgaaaa
     6541 agataacgcg caaaggggcg acagtggatg ctgcagttga agcggcactg agagagctca
     6601 atgtcacgcg taatgaagtc gatgtcgaaa ttgtcgatgc cggaaagaaa gggtttctcg
     6661 gatttggagc aagggaagcc gaagtgacca ttactgtaaa ggaaacggtc aagcaagaag
     6721 cgattgaacc tgaagagaag atagaaacga ttcaggaaga aacaatggaa tctgcaattg
     6781 aagaagcacc cgttgttgtg ccgatggaac cggtgacgca aacgatagag gtagaggcaa
     6841 tggagccgat tgcatcggat gagcaggcaa tcgatgaaac gaaagcctat atcacggaaa
     6901 ttgccaaagc gatgggcatc gacgacctgg tcgtcaagca cgaaatggat gggaaatatc
     6961 taacctttca aatggaaagc gaaaaagctg cattcctgat cggaaaacgc ggtcaaacat
     7021 tgaacgcgct gcaacagcta gcccagctag tggccaataa aacatcagag caattcaagg
     7081 tcgtgcgtct tgacgtgggc gactatcgag aacgccgcga acaatcattg gagcaattcg
     7141 cggaacggat ggccgaccaa gctgtccgga atggaaggaa agttcaactg gaaccaatgc
     7201 cttcctatga aagaaaagtc attcataacg ccttatcgaa ccgattggac atcgaaacgt
     7261 actcggaagg aacagatccg aaccgatacc tggtcatcga gccgatcagt taatccaaaa
     7321 ccccgtgaag tggtcacggg gtttttgtgt gcggtgaatt aaacttatcc acattttttt
     7381 ggatccataa ctatgagaac gaatggattt gtggtattat atgttgtcag atagtcaagt
     7441 caaaacaggt tatccacatg tgggtaaccg aagtaggaag gtgaagaatg tgcattttga
     7501 tacaatagcc gccatatcga ctccgatggg ggaaggggct atcgccatcg tccggcttag
     7561 tggagatgag gctatcggca ttgcagataa aattttccgt tctccgtcag ggaaaaaact
     7621 agcggatgaa caatcacata caatccatta tgggcatctg gcagacccgt ccacagagga
     7681 aatcgtcgag gaagtcatgg tatccatcat gaaggctcca aaaacgttca cccgtgaaga
     7741 cgttgtcgaa gtgaattgcc acggcggcgt ggtggcggtg aaccgtgtcc tcaaccttgt
     7801 gttgaaagaa ggggctcgcc ttgcggaacc aggcgagttt tccaagcgtg catttctgaa
     7861 tggacgcatc gatttatcgc aggcggaggc agtcatggat ctgattcgcg caaaaacgga
     7921 tcgggcgatg aatgtcgcct tgaatcagat ggaaggtaag ctatccaagc tgatcggtga
     7981 actgcggcag gcgcttctag aaacattggc gcaagtcgaa gtaaatattg attatccgga
     8041 atatgacgat gtcgaggaag tgaccattcc gctc
//
LOCUS       DASULI010000035         4735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1787028,
            whole genome shotgun sequence.
ACCESSION   DASULI010000035 DASULI010000000
VERSION     DASULI010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787028"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFX34_00730"
     CDS             <1..384
                     /locus_tag="VFX34_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563454.1"
                     /translation="QIVMQLPLEFLGKRIEATIQWNGRKKDDGKIDADFARILFYLEL
                     ESLNKTVVDMQVQNRVVALTVFNENDELREIGSLLQTKLRDGLESVDYRLSGVSFKNF
                     EEDRKMDSKRRNEIMTDHGGVDFRI"
     gene            381..680
                     /locus_tag="VFX34_00735"
     CDS             381..680
                     /locus_tag="VFX34_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX5563455.1"
                     /translation="MTGQKHTRKEAIALSYDPNAGDAPKVVAKGKGKIAENILDKAKE
                     HHVPIQEDSTLVELLGQLQVDETIPEQLYQAVAEVFAYVYQMDREHGARKNNSGK"
     gene            798..1958
                     /gene="sucC"
                     /locus_tag="VFX34_00740"
     CDS             798..1958
                     /gene="sucC"
                     /locus_tag="VFX34_00740"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5563456.1"
                     /translation="MNIHEYQGKQLLRDYGVAVSNGRVAFSPDEAVKAAKELGTEVIV
                     VKAQIHAGGRGKAGGVKIAKNLDEVREYAKELLGKTLVTHQTGPEGKEVKRLLVEEGS
                     DIKKEYYLGLVVDRATDRVTLMGSEEGGMDIEEVAEETPEKIFKEIIDPIVGLTGFQA
                     RRMAFNMNIPTHLVNKAAKFMLGLYQVFVEKDASIVEINPLVVTGDDNVLALDAKFNF
                     DDNALYRHKDIVELRDFDEEDPKEIEASKYDLSYISLDGNIGCMVNGAGLAMATMDTI
                     NYYGGSPANFLDVGGGATAEKVTEAFKIILSDEAVKGIFVNIFGGIMKCDVIAEGVIA
                     AAKEVGLEVPLVVRLEGTNVDKGKALLNESGLNIIAADTMADGAQKIVELVG"
     gene            1977..2879
                     /gene="sucD"
                     /locus_tag="VFX34_00745"
     CDS             1977..2879
                     /gene="sucD"
                     /locus_tag="VFX34_00745"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5563457.1"
                     /translation="MAIYINKDTKVIVQGITGSTALFHTKQMLEYGTKIVGGVTPGKG
                     GTEVEGVPVFNTVEEAVKATGANVSVIYVPAPFAADAILEAVDAELDMAICITEHIPV
                     LDMVKVKRYMEGKKTRLVGPNCPGVITSDECKIGIMPGYIHTKGHVGVVSRSGTLTYE
                     AVHQLSQAGIGQTTAVGIGGDPVNGTDFIDVLKAFNEDPETYAVVMIGEIGGTAEEEA
                     AEWVKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAAEKIKAMNAAGIEVADT
                     PSVIGETLIKVLKDKGLYEKCKTH"
     gene            2951..3859
                     /gene="dprA"
                     /locus_tag="VFX34_00750"
     CDS             2951..3859
                     /gene="dprA"
                     /locus_tag="VFX34_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5563458.1"
                     /translation="MHLTDVDRRLLALHYVYPVPFNKTAEILKLDPELRNLFTYRSDE
                     LARLLQIPNAKAVRLNEQLKRHKQTPFTLLYHREKIIPIPITNPAYPKSLKWLIDPPT
                     ILYVKGDPAILETSIKVAIIGSRKATDYSRKALSYIVPPLVDRGAVIVSGLAKGADTM
                     AHESAIHFGGKTIAVLGHGLFHLYPHENKALAEKLADHHLLLTEYPPYMKPARWTFPM
                     RNRIISGLSESIIITESEEKSGTMSTVDHALEHGKAIYAVPGPITSTLSLGPNKLIEQ
                     GAKPIWNGFQALESSPFQWREKDAFY"
     gene            4010..>4735
                     /locus_tag="VFX34_00755"
     CDS             4010..>4735
                     /locus_tag="VFX34_00755"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEX5563459.1"
                     /translation="MADYLVIVESPAKAKTIERYLGKKYKVRASIGHLRDLPRSQMGV
                     DTENNYEPKYITIRGKGPILQELKKDAKKAKKIFLAADPDREGEAIAWHLAHQLGVDI
                     DSDCRVVFNEITKDAIKESFKHPRPIDMNRVDAQQARRILDRLVGYNISPILWKKVKK
                     GLSAGRVQSVALRLIIDRENEIKAFIPEEYWSISGRFIKDGKAFDASFYGNASKKMKL
                     EDKQQVDEVLSKMSGDEFEIVNVV"
BASE COUNT         1475 a          979 c         1179 g         1102 t
ORIGIN      
        1 caaattgtca tgcagctgcc acttgaattc ctcggtaaac gcatcgaagc gacgatccaa
       61 tggaatggaa ggaagaagga cgatggtaaa atcgatgcgg atttcgcccg gatcctgttt
      121 tatttggaat tggagtcgct caacaagaca gtcgtcgata tgcaagtgca aaatcgggtt
      181 gtcgctttaa cggttttcaa tgaaaacgat gaattaagag aaatcggtag ccttctgcaa
      241 acaaagttga gggacggcct cgaatcagta gattatcgat tgtctggagt ctcttttaaa
      301 aatttcgaag aggataggaa aatggattcc aaaaggcgga atgaaatcat gacggatcac
      361 ggtggagttg acttccggat atgacaggac agaaacatac acgtaaagaa gcgatcgccc
      421 tttcctacga tccgaatgca ggggatgctc cgaaggttgt cgcaaaggga aaagggaaga
      481 ttgcggaaaa tatattggat aaagcaaaag aacatcatgt ccccatacag gaagattcta
      541 cactagtcga attgcttgga cagcttcagg tggatgaaac gattcctgaa caactatacc
      601 aagcggtggc agaagtgttt gcatacgtct atcaaatgga cagggaacat ggcgcgcgca
      661 agaataatag cggaaaatga cgaaaaatag taaaagacac tcaaatgcca acaacaattg
      721 tggacattat tttacatctt tactacaatg gatacggcag taaattatga gctacaagtt
      781 cgaatggagg atgtaagatg aatatccatg aatatcaagg aaagcagctg ctcagagatt
      841 atggcgttgc tgtttcaaac ggtcgggtag ctttctctcc cgatgaagct gtaaaagcgg
      901 caaaagaact cggtacggaa gtaatcgtcg tcaaggcgca aatccatgct ggcgggcgtg
      961 gtaaagcggg cggtgttaaa atcgccaaaa atcttgacga ggtgcgcgag tatgcaaaag
     1021 agcttcttgg caaaaccctt gtgacccatc aaacaggccc tgaaggaaaa gaagtgaaac
     1081 gacttcttgt cgaagaagga tccgatatca aaaaagaata ttatttaggt cttgttgtag
     1141 accgcgctac agatcgcgta acgcttatgg gatctgaaga gggcggaatg gacatcgagg
     1201 aagtcgcaga agagactcct gaaaagatct tcaaagaaat cattgatccg atagtcggcc
     1261 taacagggtt ccaggcacgc cgaatggcat tcaatatgaa catccctaca catcttgtga
     1321 acaaagctgc aaaattcatg ctaggactct atcaagtatt tgttgaaaaa gacgcatcga
     1381 tcgttgagat caatccactt gtcgttacag gcgatgataa tgtattggca ttggatgcga
     1441 aattcaactt cgatgacaac gcattatacc gtcataaaga tattgtcgaa ttacgcgatt
     1501 tcgacgagga agatccaaag gaaatcgaag cttccaagta cgatcttagc tacatatccc
     1561 tggatggcaa catcggctgc atggttaacg gtgcaggtct tgcgatggcg acgatggata
     1621 caatcaatta ctacggcgga tcaccggcca acttccttga cgttgggggc ggcgctacag
     1681 cagaaaaagt tacagaagcg ttcaaaatca tcctttctga cgaagctgtc aaaggaatct
     1741 tcgtcaacat cttcggcggc atcatgaaat gtgacgttat cgcagaaggc gtcattgcgg
     1801 ctgcgaaaga agtaggcctt gaagtgcctc ttgtagtccg tttggaaggc acgaacgtag
     1861 acaaaggtaa agcgctattg aacgaatcag gattaaatat catcgctgct gacactatgg
     1921 cagacggtgc acaaaagatt gttgaactcg taggctaaga aaggcaggga cagaagatgg
     1981 caatttatat taataaagat acaaaagtga ttgtacaagg tattactggc tcaacagcac
     2041 ttttccatac gaagcaaatg cttgagtatg gcacgaagat cgtcggcggt gttacaccgg
     2101 gcaaaggcgg aacggaagtt gaaggcgtac ctgtcttcaa tacggttgaa gaagcagtaa
     2161 aagcaacagg tgcaaatgtt tccgtcatct acgttccggc tccgttcgca gcagatgcga
     2221 tccttgaagc agttgacgcg gaattggaca tggcaatctg tattactgaa catattccgg
     2281 ttcttgacat ggtaaaagtg aagcgctaca tggaaggcaa gaaaacacgt cttgtcgggc
     2341 cgaactgccc gggtgtcatt acatctgatg agtgtaaaat cggcatcatg cctggctaca
     2401 tccatacaaa aggccatgta ggcgtcgtat cccgttcagg aacattgacg tatgaagctg
     2461 ttcatcagct ttcgcaagca ggcatcggcc agacgactgc tgtcggaatc ggcggagacc
     2521 ctgtaaacgg gacggacttc attgacgtac taaaagcatt caacgaagac ccggaaacct
     2581 atgcggtcgt catgatcggt gaaatcggcg gtacagctga agaagaagca gctgaatggg
     2641 tgaaagcgaa catgacgaag ccggttgtcg gcttcatcgg cggtcagact gcacctccag
     2701 gaaaacgtat gggccatgct ggcgcaatca tttccggcgg taaagggaca gctgcagaaa
     2761 aaatcaaggc gatgaatgct gcaggaatcg aagtagctga cacaccatcc gttatcggcg
     2821 aaacacttat caaagtgttg aaagataaag gactttacga aaaatgtaaa acacattgat
     2881 gatccgtacg gcatgcagga aactgcatgc tgttcttttc atatccggaa attgaagaag
     2941 gaggaatttt gtgcacttga ccgacgtaga ccggaggctg cttgccttgc actatgtgta
     3001 tcctgttccg tttaataaaa cggcggaaat tttgaagttg gaccccgaac ttcgtaatct
     3061 gttcacttat cgctccgacg aactggcgag actgctgcaa atccccaatg caaaagcggt
     3121 ccggctgaat gaacagctga aacggcacaa acaaacccct tttacccttc tctatcatcg
     3181 tgaaaaaatc attcctatcc caattacgaa cccggcctat ccgaagtcgt tgaaatggct
     3241 tatcgaccct ccaaccatcc tttatgtcaa gggtgatcca gcaatattgg aaacttctat
     3301 taaagtggcg atcatcggtt ccagaaaagc aactgactat tcacgtaaag cgctttcata
     3361 catcgttcct ccccttgtag accgaggagc agtgatcgta tccggcttgg cgaaaggcgc
     3421 cgatacgatg gcgcatgaat ccgcaatcca ctttggcgga aaaacgattg ccgtccttgg
     3481 acatgggcta ttccatttgt atccgcatga aaacaaagcc cttgcggaaa aactggccga
     3541 tcatcattta ttgctgacag aatatcctcc ttacatgaaa cccgcaagat ggacttttcc
     3601 aatgagaaat cgaatcatca gcggtttatc ggaatcgatc atcatcaccg aatcagagga
     3661 aaagagcggc acaatgagca ctgtcgatca tgcactcgag catgggaagg ccatttatgc
     3721 ggttcctggc ccgattacct cgactttatc acttgggccc aataaattga ttgaacaagg
     3781 tgcaaagcct atctggaatg gctttcaggc acttgaaagt tccccatttc aatggaggga
     3841 aaaagatgcc ttttattgaa aaggttgatt ttaaatcaaa tctgttatac attttgcaac
     3901 agatattctt ttatatccgg attcacctgg aacaggtttg ggcaagaata aaaagaaaat
     3961 gcccttcgga tgtataaaaa gggaacctct atgaggagga acatgaaaaa tggcagatta
     4021 cttagtaatt gttgagtcgc cggccaaggc gaaaacaatt gaacgttatt tagggaagaa
     4081 gtacaaggtt cgtgcctcca tcgggcactt gcgggatttg ccgcgcagtc agatgggtgt
     4141 ggataccgaa aacaattatg aaccaaaata catcacgata cgcggaaaag gccctatatt
     4201 gcaagagttg aaaaaagacg ctaaaaaagc gaagaaaata tttctcgcgg ctgaccccga
     4261 cagggaaggg gaagcgatcg catggcattt ggcacatcaa ttgggcgtcg atatcgattc
     4321 ggattgccga gtcgttttca atgaaatcac aaaggatgcc attaaggaat ctttcaaaca
     4381 cccgagaccg attgatatga atcgggtgga tgcccaacag gcgagaagga tattggatag
     4441 actcgtaggc tataatatta gtccgatcct ctggaagaaa gtgaagaaag gattgtcagc
     4501 cgggcgtgtg caatcggtcg ctttgcgatt gatcatcgat cgggaaaatg aaatcaaggc
     4561 attcataccg gaagaatatt ggagcattag cggaagattc atcaaggatg gaaaagcatt
     4621 cgacgcttcc ttctatggta atgcatcgaa gaaaatgaag ctggaagata agcagcaggt
     4681 agatgaagtc ctatccaaaa tgtcaggcga cgaatttgaa atcgtcaatg tcgtt
//
LOCUS       DASULI010000036         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1650123,
            whole genome shotgun sequence.
ACCESSION   DASULI010000036 DASULI010000000
VERSION     DASULI010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650123"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFX34_00760"
     CDS             <1..588
                     /locus_tag="VFX34_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5563460.1"
                     /translation="LIKFREQEWNLVLLDLMLPGMNGLDVLRRIRAAEDGTPVILLTA
                     KSEVEDKVTGLDLGANDYVTKPFEIEELLARVRSAIRTSRKGNVVKDEYLHEYAGLSI
                     HEQTREVKRNGESIDLTPREYDLLLHLIRHPNQVLSREQLLDAVWGFDYYGDTNVVDV
                     YIRYVRKKIDQQNEPSLIQTVRGVGYALKEPIHEA"
     gene            578..1936
                     /locus_tag="VFX34_00765"
     CDS             578..1936
                     /locus_tag="VFX34_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5563461.1"
                     /translation="MKLKQKIYVFSTLLMFILLVLANILIYFFFERMSHNTEYEQMIG
                     RAKELTVAMSKLETEMDAGILLRAFVPTNGAVSIVDSNNKMLINVQSLAGLGQAGSQV
                     KPGNSYTIDRFEGTEALTISMPIIWPTGEVVTMRMTQLLVDVEENMRGLKYVLIGVTT
                     FAMIPITISSMTLGRIVTRPIDNLITAMNRSRQSGTYEKLTGVVNGNDELAQMERTFN
                     EMMEQLEQNFMKQEQFVSNASHELKTPLTVIESYSRLLARRGFENREVVDEALNAIIS
                     ETGRMKEMIAQMLDLAKSSGSEVFEFAQVDLTPLLEGAVKPLRQAYGREIAIEADRPM
                     IVETDGKFVKQLLFILLDNALKYSDKEIFTSIEDNEDEVVITVKDHGKGIPKSDLPHI
                     FDRFYRVESDRSRKTGGTGLGLSIAKEIADALGAKLTIESVVDMGTVIRVTIPKIREG
                     SQ"
     gene            1983..2687
                     /locus_tag="VFX34_00770"
     CDS             1983..2687
                     /locus_tag="VFX34_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX5563462.1"
                     /translation="MNIWKKPWFIPIVLTIIIVVAGQLYTAGKLTKAEALPKEEISSQ
                     LAMIYDGEVEDLMLDGSVYKAKISKAGAEYAVEVEAESGKVLSLIQTKEISKPDIVMD
                     TDEGEIAQVEASPDETGKTDSEVPEDALNKATDEKTEMPKPNGNKTPEQSKPAKQEKS
                     KKTVLISEQEAVKIGMAQLPAGMVGEVDDVDFINSVDGGYYLVQIDIDTDDDMDEVTY
                     QIHAISGKVMTVTWDD"
BASE COUNT          860 a          581 c          821 g          692 t
ORIGIN      
        1 ctgatcaaat tcagggagca ggaatggaat cttgtgctac tggatctcat gctgcctggc
       61 atgaacggat tggacgtgct tcggcggatc cgggcggctg aagatggcac gcctgttatt
      121 ttattgaccg cgaaaagcga agtggaagat aaagtgacag ggctggactt gggagcgaat
      181 gattatgtca cgaagccgtt tgaaatcgaa gaattgcttg cccgcgtccg ttcggccatt
      241 cgaacttccc gaaaggggaa tgtcgtgaaa gatgaatacc ttcatgaata tgccggatta
      301 tcgattcatg agcaaacccg tgaagtgaag cggaatggtg aatcaatcga tttgacccca
      361 agggaatatg atttattgct tcatttgata cgacatccga atcaagtgct ttcacgtgaa
      421 cagctgctcg atgcggtatg gggtttcgac tattacggag atacgaatgt ggttgatgtt
      481 tatatccgtt atgtccggaa gaagattgat caacagaatg aaccttcgct cattcaaacc
      541 gtccggggcg tcgggtatgc attgaaggaa ccgatccatg aagcttaaac agaagatcta
      601 tgtcttttct actcttttaa tgttcattct tctcgtgctc gcaaatatcc tcatctattt
      661 ctttttcgaa aggatgtccc ataatacgga atatgagcag atgattggac gggcgaagga
      721 actgaccgtg gccatgagca aattggagac ggagatggat gcaggcatcc tgttgcgtgc
      781 attcgtccct acgaatggcg ccgtttctat cgttgacagc aataataaga tgctcatcaa
      841 tgtccaatct ttggcggggc tcgggcaagc cggcagccaa gtgaagccgg gcaattcata
      901 tacgattgac cgattcgaag ggactgaagc attaacgatt tcaatgccga tcatttggcc
      961 gacaggtgaa gtcgtcacga tgagaatgac acagctgctc gtagacgttg aggaaaatat
     1021 gcggggcttg aagtatgtcc tgatcggcgt gacgacattt gcgatgattc cgatcaccat
     1081 ttccagtatg acgcttggaa ggattgtcac ccggccgatc gacaacctca taacggcgat
     1141 gaaccgaagc cggcagtccg ggacgtatga aaagttgacc ggtgtcgtga acggaaatga
     1201 cgagcttgcg caaatggagc ggacgttcaa tgaaatgatg gaacaattgg agcagaattt
     1261 catgaagcag gagcagtttg tctcaaatgc ctcgcatgaa ttgaaaacgc cgttgaccgt
     1321 cattgaaagc tattcccgat tgttggcaag gcgcggcttt gaaaaccggg aagtcgtcga
     1381 tgaggctttg aatgccatta tctctgaaac gggcaggatg aaagaaatga ttgcgcaaat
     1441 gctcgacttg gcgaagagca gcggatcaga ggtgttcgaa ttcgcgcaag tcgatttgac
     1501 tccgttgctg gaaggggcgg tcaagccatt gagacaggca tacggcaggg agattgccat
     1561 cgaggcggac aggccgatga tagttgaaac ggacgggaag tttgtgaaac aactgttgtt
     1621 cattctccta gacaatgcgc ttaaatatag tgacaaggaa atctttactt ccatcgaaga
     1681 caatgaggac gaagtggtca tcacagtgaa agatcatgga aaagggattc caaaatcgga
     1741 tttgcctcat attttcgacc gtttttaccg tgtggagtcc gaccgtagcc ggaagacggg
     1801 cggaaccggg ttagggcttt ccattgcaaa ggagattgcc gatgcacttg gcgccaaatt
     1861 gacgattgaa agtgtcgtgg acatgggaac cgtcatccgg gtgacgattc ctaaaataag
     1921 ggaaggttct cagtgaattt taatgttagg gcagtaagat gaaggaaagg aggatttttt
     1981 gcatgaacat atggaaaaaa ccttggttca tcccgattgt cttgacgatc atcattgttg
     2041 ttgcggggca gctgtacacg gcaggaaaac tgacgaaagc ggaagccttg ccgaaagagg
     2101 aaatcagttc ccaattagcg atgatttatg atggtgaagt ggaagatctc atgttggatg
     2161 gatctgtata taaagcgaaa atttcaaaag caggtgcgga atacgccgtg gaagtcgagg
     2221 cggaatcggg aaaagtcctt tcgctcatcc agacgaaaga aatttcaaaa cccgatattg
     2281 tgatggatac agatgaaggg gaaatagccc aagttgaagc atcgcctgac gaaacgggta
     2341 aaacggacag tgaagttccg gaagatgcgc taaacaaagc gacagatgaa aaaacggaaa
     2401 tgccgaagcc gaatggaaac aaaacacctg agcaatccaa gccggcgaag caggaaaagt
     2461 cgaagaagac tgttctcatc tccgagcagg aggcagtgaa gatcggaatg gcccaattgc
     2521 cggcgggaat ggttggcgag gtcgatgacg tggattttat caattcggtg gatggcggct
     2581 attatttggt gcaaatcgat attgacacgg acgatgatat ggatgaagtc acctatcaaa
     2641 tccatgcgat ttcaggaaaa gtaatgacag tcacttggga tgattaattc tctcatcaaa
     2701 ttctaatatt cctctcaatg tcctctcatc ttcgcgaggt agtatgaaga tagaacaaaa
     2761 aaggaggaaa acgaacatga aaaaatggat gttgatccct gcagtggcag gcgtactagc
     2821 tatcggcggc gttgcactag cggaggattc tgtaccaagc gtctcgaagc aggcggataa
     2881 gatgatttcc ttacaggaag cgaaacaggt cgccactgac aaagtgaaag gcggcaaagt
     2941 gacggaaatc gagt
//
LOCUS       DASULI010000037         3773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_261166, whole
            genome shotgun sequence.
ACCESSION   DASULI010000037 DASULI010000000
VERSION     DASULI010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261166"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..618
                     /locus_tag="VFX34_00775"
     CDS             91..618
                     /locus_tag="VFX34_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEX5563463.1"
                     /translation="MKMVLIIFVINIVYVSFFTIRMILTLKGYRYSAAFVSMIEIVIY
                     IVGLGLVLDNLNEIQNIIAYALGYGSGVIVGTKIEEKMALGYITVNVITAGIDFEMPR
                     LLREKGYGVTDWSAHGLEGSRSAMQILTPRKDELKLYDTIKQIDPKAFIIAYEARTIH
                     GGFWVRSVRRGNLFK"
     gene            615..806
                     /locus_tag="VFX34_00780"
     CDS             615..806
                     /locus_tag="VFX34_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HEX5563464.1"
                     /translation="MSAKKKTVWFEVAEGESIEECLQRLAAEGYRVAGRMEEPIFMEK
                     DGEYVPIRQVIKFKGVLEE"
     regulatory      894..994
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1166..1654
                     /gene="purE"
                     /locus_tag="VFX34_00785"
     CDS             1166..1654
                     /gene="purE"
                     /locus_tag="VFX34_00785"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5563465.1"
                     /translation="MGPKVGVIMGSKSDWETMKHACDILDELGVMYEKKVVSAHRTPD
                     FMFEYAEQAQDRGIEVIVAGAGGAAHLPGMVAAKTLLPVIGVPVQSKALNGMDSLLSI
                     VQMPGGVPVATVSIGKAGATNAGLLAAQFLAVHDSELRNRLEERREKMRDAALESSGE
                     LQ"
     gene            1651..2769
                     /gene="purK"
                     /locus_tag="VFX34_00790"
     CDS             1651..2769
                     /gene="purK"
                     /locus_tag="VFX34_00790"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX5563466.1"
                     /translation="MKTILPGQTIGIIGGGQLGRMMGLAAKEAGFKIAVLDPTMDSPC
                     GQIADIRIVAPYDDEAALEELGEVSDVITYEFENIDFEGLERLSHIAYVPQGAELVRI
                     TQNRIAEKAEIKASGAPVANYVAAASFSELIKEIDKVGFPCIVKTAFGGYDGKGQVKL
                     ESADDIELAEPLFAHSACIAEAFVPFKTEISAIIQRNPEGESYCLPIAENIHKNHILH
                     ESIVPARVAESVIAKAEDAAEKIADHLDLVGTLAVEMFVLENDEIIINELAPRPHNSG
                     HYSIEACNVSQFHQHIRAVCGWPLRKPKLWAPSIMVNVLGEHVVPLKKVITDHPDWSV
                     HLYGKAEAKEKRKMGHVTIMTDCLEKTLEEIESTGIWA"
     gene            2903..3157
                     /gene="purS"
                     /locus_tag="VFX34_00795"
     CDS             2903..3157
                     /gene="purS"
                     /locus_tag="VFX34_00795"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017419024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5563467.1"
                     /translation="MTKVNVYVTLRESVVDPQGIAAQEALQTLGFNEVESVRIGRLIE
                     LELAGTQSEIEARVKEMCDQLLVNKVIEDYSFEIGEVAGK"
     gene            3154..>3773
                     /gene="purQ"
                     /locus_tag="VFX34_00800"
     CDS             3154..>3773
                     /gene="purQ"
                     /locus_tag="VFX34_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5563468.1"
                     /translation="MRFAILSFPGSSCDVDMYHALVDNVGVGAEIVSHQEANLENFDA
                     VVIPTGASFGDYLRPGALAQSSKSIDSLKAFAASGKLVLGVGNGFQILVEAGILPGAF
                     LRNKGLKFRSGNARLAIQNSQSSFTSIYEQGQQITLPFAHEYGNYFVDADTAEQLKEQ
                     NRIVFTYEDGNPDGSVERIAGVLNEEGNVLGMMPLPERAVEEIIGGT"
BASE COUNT         1018 a          766 c         1045 g          944 t
ORIGIN      
        1 tcgattggaa agcgtccgcc tgtaacgtaa atcaacgacg gatagctgga gatatatttt
       61 caacctagtc atcaggaggt ttgctgaaga atgaaaatgg tattgatcat atttgttatt
      121 aacatcgtgt acgtatcctt ctttacgatc cggatgattc ttacactgaa gggctatcgt
      181 tattctgccg cctttgtgag tatgatcgaa atcgtcatct atattgtagg tcttggattg
      241 gtattggata atctgaatga aatacaaaat ataattgctt atgcgctagg atacggtagt
      301 ggagtcattg tcgggacgaa gatcgaggag aagatggcgc tcgggtatat cacggtgaat
      361 gtcattacgg ccggcatcga ctttgagatg ccccggctgt tgcgcgaaaa agggtatggg
      421 gtgacggatt ggtctgcgca tggattggaa ggcagccgtt cagcgatgca gattttaacg
      481 ccgcgaaaag atgagttgaa actatatgat acgatcaaac agattgaccc gaaagcattt
      541 atcattgcat atgaagctag gacaatccat ggaggcttct gggtgagatc tgtgaggaga
      601 gggaatttat ttaaatgagt gcgaaaaaga aaacggtatg gtttgaagtt gcggaaggcg
      661 aatccatcga agaatgcttg caacggctgg cagcggaagg gtaccgtgtg gcgggtcgga
      721 tggaggaacc gatcttcatg gagaaagatg gtgagtatgt cccaatccgg caagtcatta
      781 agtttaaggg ggttttagaa gaataaattg tctattatac gaacgattaa catatgcaga
      841 tgtttatcgt tcgtattttt tattgacgta ttcaagtaca gttgttacac tgagtgtgta
      901 agtaagataa cccacatata tgctgaagga ttggcttcag cgtttctacc aggacgccgt
      961 aaatgtccgg actatgcggg aaagcgattt ttaggattta gcgaaaggtg tgccatatct
     1021 tatggatacg ttcatcttga gcattttaat atccttgaat gaactgcttt ccatgcattt
     1081 tggaaggcag cttattcaag gattttttaa tggaaaatta cctcagttac attcattatc
     1141 agtctactaa aggagcggat tgtctatggg accgaaagtc ggcgtgatca tgggaagtaa
     1201 aagcgattgg gaaacgatga agcatgcttg cgacatattg gacgagttgg gcgttatgta
     1261 tgaaaagaag gtcgtttcag cgcatcgcac accggacttc atgttcgaat atgcggaaca
     1321 ggcacaggat cgcgggattg aagtgatcgt agcaggtgca gggggagctg cccatcttcc
     1381 cggaatggtc gctgcgaaga cattattgcc tgtcatcggt gttccggttc aatcgaaagc
     1441 attgaatggt atggactcat tgctttccat cgtgcaaatg ccagggggag ttcccgtcgc
     1501 taccgtatcg atcggcaaag ccggtgctac gaatgccgga ctactggccg ctcaattttt
     1561 agcagtccat gattcggagc tgcgcaatcg tcttgaagaa cggcgggaaa aaatgcggga
     1621 tgcggcattg gaaagcagtg gtgagttgca atgaagacaa ttttacctgg acagacaatc
     1681 ggcattatcg gtgggggtca gctcggccgg atgatggggc tcgcagcgaa ggaagccggc
     1741 ttcaagattg ccgtgctcga tccgacgatg gattcgccgt gcgggcagat tgcagatatc
     1801 cgtatcgtcg ctccgtatga cgacgaggca gcgctcgagg aactcggtga agtaagtgat
     1861 gtcatcacat atgaatttga gaacatcgat tttgaaggtc ttgagcggct tagccatatc
     1921 gcttatgttc ctcaaggagc ggagcttgtt cggatcacac aaaatcggat agcggagaaa
     1981 gccgaaatca aagcttccgg tgcgccggtc gcaaattatg tcgctgccgc ttcattcagt
     2041 gaattaataa aagaaatcga taaggtcggc ttcccatgca tcgtgaaaac cgctttcggc
     2101 ggatatgacg gaaagggtca ggtgaagctc gagtcggcgg acgatatcga actggcggaa
     2161 ccgctatttg cccactcggc ttgcatcgca gaagcgttcg tacctttcaa gacggaaatc
     2221 tcagctatca tccagcgcaa tcccgagggt gagtcgtatt gccttccgat tgctgaaaac
     2281 attcataaga atcatatttt gcatgaatca atcgttccag cgcgggttgc cgagtcagtc
     2341 atcgcaaagg ctgaagatgc agcggaaaaa atcgcagacc atcttgacct tgtcggaaca
     2401 ctggcggttg aaatgttcgt gcttgagaac gatgaaatta ttatcaatga acttgctcct
     2461 cgtccgcata attcgggcca ttactcgatt gaggcgtgca atgtatcgca gttccatcag
     2521 catatccgtg cggtgtgcgg ctggccgctg cgcaagccga agctatgggc accgtccatc
     2581 atggtgaacg tgctagggga gcatgtcgta ccgttgaaga aagtgatcac ggatcatcct
     2641 gattggtcgg tccatctgta cgggaaggcc gaggcgaaag aaaaacggaa gatggggcat
     2701 gtgaccatca tgaccgattg tttagagaag acgttggagg aaatcgaatc gacaggcatt
     2761 tgggcttaac ttactagtaa gttaaagcct ccggcggatg tcacagattt tgaaaaatcc
     2821 ggacgcaatt acgccaaggc gttattgatt gattttcaaa ctaattcgat acaaaactta
     2881 atccattcgg gaggaaattg acatgacaaa agtgaacgtt tacgtaacat tacgcgaaag
     2941 tgttgttgat ccacaaggaa ttgcagctca ggaggcgctc caaacattgg ggttcaatga
     3001 agtcgaaagc gtccgcatag gaaggctgat cgagcttgag ctggcaggaa cacaaagtga
     3061 aatcgaagcg cgcgtgaagg aaatgtgcga tcagcttctt gtcaataaag tgatcgaaga
     3121 ctatagcttt gaaatcgggg aggttgcggg caaatgaggt tcgctattct aagtttcccc
     3181 gggtccagct gtgacgtgga catgtatcat gcccttgtcg ataacgttgg cgtcggcgcg
     3241 gaaatcgtca gccatcaaga agccaacttg gaaaatttcg atgccgtcgt cattccgacg
     3301 ggcgcttcct ttggcgacta tttacgccct ggggcgcttg cacaaagctc caaatcaatt
     3361 gatagcctaa aagcattcgc tgcttccggc aagctggtgc tcggcgtagg gaacggtttt
     3421 cagatccttg tcgaggcggg aatccttcct ggcgctttct tgcggaataa aggattgaaa
     3481 ttcagaagcg gcaatgcgcg gctcgctatt caaaattcgc aatcatcatt tacgagcatc
     3541 tatgagcaag ggcagcaaat tacgcttcct ttcgcacatg agtacggcaa ttatttcgtc
     3601 gacgcggaca ctgcagaaca attgaaagag cagaaccgca tcgtgttcac atacgaagac
     3661 ggaaatccgg atggcagcgt ggagagaatc gcaggcgttt tgaatgaaga agggaatgtc
     3721 ctcggcatga tgccgcttcc tgaacgtgcg gtagaggaga tcatcggcgg aac
//
LOCUS       DASULI010000038         4292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1889398,
            whole genome shotgun sequence.
ACCESSION   DASULI010000038 DASULI010000000
VERSION     DASULI010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889398"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1058
                     /gene="nadX"
                     /locus_tag="VFX34_00805"
     CDS             276..1058
                     /gene="nadX"
                     /locus_tag="VFX34_00805"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX5563469.1"
                     /translation="MIGKGNLGTYLLQKINKEQLLPNTKITAILDEREKARVELPAYA
                     TEYECAAFHDIDSFLQSDIDIVVECSNIEAAKEYSLKVIEKKDLMLISIGAFADYSSF
                     GNQVEQAAKTSGNRVYLPSGAIGGLDVLKAAKFAGGLESVTLTTRKPAHSLTAEEIPA
                     EQTLFEGPARDAIEQFPKNANVAIVLSLAGIGVEKTQVRIIADPAVTQNIHHITAKGS
                     FGEFDMTIKNNPSPDNPKTSHITGASILAALANINNAVVVGS"
     gene            complement(1066..1875)
                     /locus_tag="VFX34_00810"
     CDS             complement(1066..1875)
                     /locus_tag="VFX34_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010284341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HEX5563470.1"
                     /translation="MDNSFQKETLDELHFWLQILGDHGRFIHDALAPGQTAWIDTASA
                     FIDRFDKLLDRSKQSLDQNELLSLVGLSKTESEAICEFKLAILKEQLVGNIKISLPPP
                     FVNHMVNEVEEAIRVLAHLEKGESPLTVHPLHHDLLWLLDAAGHAGTIDANMDRVEKQ
                     LKKKSRKFLKEWEAFYLKAVELTGFLRTNATKFPALDKFHNDVYLEMTIFKTFLNELE
                     VMGLKKETLGVFTPLMADHMAREECYYLLKLAESAGTPDPDCNPAKERVQG"
     gene            2042..2953
                     /locus_tag="VFX34_00815"
     CDS             2042..2953
                     /locus_tag="VFX34_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5563471.1"
                     /translation="MVKQAVEQVTDDHVVKALEKNLAIIRYDLDHKIAYVNDIFANAM
                     GYTKDEMIGVRHDDLCFDEYVKSAEYDKFWRELLRGISQHGKIEMKAKNGEKVWLEAT
                     YMPVFSHDGRKVISISKVASNITKRQTSVTSVVEELRQMAEGLTERADIGIERSKELL
                     VIIEDIAAISHDNTKTLGNLQLHAESIQHIVKTIREIAAQTNLLALNAAIEAARAGEH
                     GRGFDVVAKEVRKLSANVEKSIVEVREGVEAITTEIKNISQGTTLAQENVDRCQQRIE
                     VAMEDFLAIASSASELDNQSRKVSSIV"
     gene            complement(3127..3963)
                     /locus_tag="VFX34_00820"
     CDS             complement(3127..3963)
                     /locus_tag="VFX34_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX5563472.1"
                     /translation="MEWKGRRGSRNVEDRRGMGGKTLVGGGIGGIVMLLLVMFLGGDP
                     GDILNNLTTSPSNTEYVETEQDKELAEFVSVVLADTEEVWTEVFEKEGMVYREPVLVL
                     YSGSVQSACGIAGSAVGPFYCPGDEKLYIDLSFYDELQTQFKAPGDFAMAYVIAHEVG
                     HHVQKLLGISDEMARIRSQVSEEEYNKYSVRLELQADYLAGVWAHHAKGMGVLEKGDL
                     EEALNAASAVGDDTIQKRARGYVVPESFTHGTSEQRKKWFYRGFEAGNLEEGDTFGAG
                     NL"
     gene            complement(4094..>4292)
                     /locus_tag="VFX34_00825"
     CDS             complement(4094..>4292)
                     /locus_tag="VFX34_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YusW family protein"
                     /protein_id="HEX5563473.1"
                     /translation="EVALHGKKALKKITQAFEKMEPDPEAEETELIKKVAEAFGFKDY
                     KMIRVEITFKGYDSKEIMFSK"
BASE COUNT         1197 a          994 c          970 g         1131 t
ORIGIN      
        1 gcatggtcgc gggggctagt gtcgccggcg gaatgacgat tgcattagga catgccgtca
       61 aatatgcaca tgaaaatgat attgaactga ccccgaaatc gttaaaaccg atctatcaga
      121 tgttcttgaa aaaggcgaat aaaaaagaga aggaaaaata atcattcagg aaagccttaa
      181 tacggtgtac ccagtagatc ccctttgccc aagccaaggg ggtttcctgt atactagttt
      241 aaaatgcaaa aggactgatt cgaatcaaaa taggcataat cggaaaagga aatcttggta
      301 catatcttct tcagaaaata aacaaagaac aacttctacc caacactaaa atcaccgcaa
      361 ttttagatga gcgcgagaaa gctagagttg aattgccagc ttacgctaca gagtacgaat
      421 gtgcagcatt ccatgatatc gattcattct tacaatcaga catagacatc gtagtagaat
      481 gctcgaatat cgaagcggct aaagaatatt cgcttaaagt tatcgaaaag aaggatctga
      541 tgttaatcag catcggggcg ttcgccgatt attcttcctt tggcaatcag gtagaacagg
      601 cggctaaaac tagtggaaac cgtgtctact tgccgtccgg tgcgattggc ggactagatg
      661 tcttgaaggc agccaagttt gcaggcggtc ttgagtctgt tactttaacg acccgaaaac
      721 ccgcccattc actcaccgca gaggaaattc ctgcggaaca aacgctgttt gaaggccctg
      781 cccgtgacgc gattgaacaa tttcccaaaa atgcgaatgt tgcaatcgtc ttgtcgttgg
      841 cgggcattgg tgttgaaaag acccaggtcc gcataatagc cgatcctgct gtgacgcaaa
      901 atattcatca tattacagcg aagggatctt tcggcgagtt cgatatgaca ataaaaaaca
      961 acccgtcccc ggataatccg aagacgagtc acataacggg agcgagtatt ttagctgcgc
     1021 ttgcgaatat caacaatgca gtggtagtcg gttcatagaa gcctattacc cctgcaccct
     1081 ctctttcgcc ggattgcaat cagggtccgg cgtgcctgcc gattctgcta gtttcagtaa
     1141 ataatagcac tcctcgcgcg ccatatgatc ggccatcagc ggggtgaaaa ctcctaacgt
     1201 ctcttttttc aaccccatta cttcaagttc attcaggaac gttttgaaga tggtcatttc
     1261 caagtaaaca tcattatgga atttatccaa ggcgggaaat ttcgttgcgt tcgtccgcaa
     1321 aaatccggtc aactcaaccg ccttcaagta gaatgcttcc cattccttca ggaatttcct
     1381 gcttttcttc ttcaactgtt tttccacgcg gtccatattt gcgtcaatcg tgccggcgtg
     1441 accagccgcg tcaagcagcc atagcaaatc atggtgaagc gggtggactg taagagggga
     1501 ctcgcctttt tccaaatggg caagaacccg gatcgcttcc tcgacttcgt tgaccatatg
     1561 attgacaaat ggcgggggca atgaaatttt aatattgcca accaattgct ccttaagaat
     1621 ggcgagcttg aattcacata ttgcttcact ttccgtttta gaaaggccga ccaacgataa
     1681 caattcattt tgatccaaag attgttttga ccgatccaat aatttatcaa aacgatctat
     1741 gaatgccgaa gcggtgtcaa tccaggctgt ttgcccaggt gcaagtgcat cgtgaatgaa
     1801 gcggccatga tctcccaata tttgaagcca aaaatggagt tcatccaaag tctctttttg
     1861 aaatgaatta tccaaagctt cacctcctga tgttctactt actattctat ggacgaagaa
     1921 ataaattaga acgactgatt cgaaggtatt ccacttcact cccgattatg gttaaatagt
     1981 aataggtcag atattgcgga caatagagaa aaatcagtgc ttgaagttgg agggggatat
     2041 aatggtgaaa caggcagtag aacaagtgac ggacgatcat gtggtgaagg cgcttgagaa
     2101 gaatctggcg atcattcggt atgatttgga tcataaaatt gcttatgtca atgatatttt
     2161 tgcaaatgca atgggctata cgaaagatga aatgatcggt gtccggcacg atgatctttg
     2221 tttcgatgag tatgtgaaga gtgcggagta cgataagttc tggcgtgagt tgctgcgagg
     2281 cataagccag catgggaaaa ttgaaatgaa agctaagaat ggcgagaaag tatggcttga
     2341 ggctacgtat atgccagtat tcagtcacga cggcagaaag gtgatcagca tttccaaagt
     2401 cgcctcgaat attacgaagc ggcaaacgtc cgttacatcc gtcgttgaag agttaaggca
     2461 gatggcggaa gggttgacgg aacgtgcgga tatcggtatt gaacggagca aggagttgtt
     2521 agtgatcatc gaagatattg cggcgatctc ccatgataat acgaaaactc ttgggaatct
     2581 gcagttgcat gccgaaagca tccagcatat cgtgaagaca atcagggaaa tcgcagctca
     2641 aacgaattta ctcgctttga atgcggctat tgaagcagca cgggcagggg agcatggaag
     2701 gggcttcgat gttgtagcga aagaggtccg taaattgtcg gcaaacgtgg agaaatcgat
     2761 agtcgaagta cgcgagggag tggaagcaat cacgacggaa atcaagaaca tatcccaagg
     2821 aacgacgctg gcccaggaaa acgtggaccg ttgccagcag cggatcgaag tggcaatgga
     2881 agatttcctg gcgattgctt cgtcggcgtc cgaattggac aatcaatcga gaaaagtgtc
     2941 gagtattgtg tgagacataa aacaacaggt gagggatcag ctccttcacc tgtttctttg
     3001 ttcattcatg taagggtcca ttcttttgca caaagctcat ttaatcgcgc attggctcca
     3061 ttattcacac aagggaaggg ctccattatt cccgaaaaag ctcaattcct ttcaagaaaa
     3121 ccctaatcaa agattccccg caccaaacgt atctccttct tccaggttgc ctgcctcaaa
     3181 tcctcggtaa aaccatttct tcctctgttc agaagttcca tgagtgaagc tttccggcac
     3241 tacatagccg cgggctcttt tctggattgt atcatccccg actgcgctcg cagcattcaa
     3301 tgcctcttcc agatcgcctt tctccagaac gcccatccct tttgcatgat gcgcccagac
     3361 gccggcgaga taatccgctt gcaattccag gcgtacagag tatttattgt actcctcctc
     3421 actgacctgg ctgcgtattc tcgccatttc atcgctgatg cctaaaagtt tctggacgtg
     3481 gtgcccgact tcatgtgcaa tgacatacgc catcgcgaaa tcaccgggcg ccttgaactg
     3541 ggtttgcagt tcatcataaa aactcagatc gatgtataat ttctcgtccc ctggacaata
     3601 aaacggaccg actgccgatc ctgcgattcc acatgcggat tggacacttc ccgaatagag
     3661 cacgaggacc ggttcgcgat aaaccattcc ttccttctca aaaacctctg tccatacttc
     3721 ttcggtatct gccaaaacga ccgaaacaaa ttcagccagt tccttatcct gttccgtttc
     3781 aacatattcc gtatttgaag gggaggtagt caaattgttg aggatatcac ccgggtctcc
     3841 gccgagaaac atgacgagga gcaacatgac gataccgccg atgccgccgc cgacgagggt
     3901 ttttcctccc atccctctac ggtcttccac attcctgctg cctcttctgc ctttccattc
     3961 catttacgtt gcctccttga aaaacatcga tgactataca agtggttagt ttatgcttta
     4021 ccctttttca acgacttata ggcagataaa gaaaaagata tcgaaatccc ttcggaatcc
     4081 gataccctat acgttatttg gagaacatga tttctttact gtcatacccc ttaaatgtga
     4141 tttcaacccg gatcatttta taatccttga atccaaacgc ctctgcaact ttcttgatca
     4201 attctgtttc ttccgcttcc gggtcaggct ccattttttc gaatgcttgt gttatttttt
     4261 taagcgcttt tttgccgtgc agcgcaactt cc
//
LOCUS       DASULI010000039         5685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_900189, whole
            genome shotgun sequence.
ACCESSION   DASULI010000039 DASULI010000000
VERSION     DASULI010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900189"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..1629
                     /locus_tag="VFX34_00830"
     CDS             412..1629
                     /locus_tag="VFX34_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5563474.1"
                     /translation="MKHVIAFDVSMGKSTMVIYDHNHCCRYEGELEHTVSGFNSLKER
                     IKLLSELDGQVPDIVFEATGVYSKGLEGFLQAQHYPYIRLNPLEAKLQMATMRRHKTD
                     ISDAHELAKSHFKSDRQETYVQEDYFEQMRALGRYYDDLEKEIRHNYNRLHAFLQLSF
                     PLFEKVFLKNSAMFLNIVQLYPHPACLLKLSKEELGHQIKRATRKIISTQKAEEKAVL
                     LIAAAKNSYSAIGPEDVRCEHLRHYAKRIADLVNQKKDIIKQMVELSKERVDFQVLVS
                     FPGIGETTAVRVIGELGDIRRFKNPKQINAYAGIDIQRHQSGKLQYQDRINKRGNRRL
                     RKILYFMVMSMISLRAKTKNTIVDYYDQLKKQPQSKPHKVAVIACMNKFLKVAFHLIH
                     HDVLYHYETAKVS"
     gene            complement(2058..5123)
                     /locus_tag="VFX34_00835"
     CDS             complement(2058..5123)
                     /locus_tag="VFX34_00835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX5563475.1"
                     /translation="MVLVYPQIVTGADLLRGIIKLDHLAPLLQKRGARSVGIVNSKMY
                     GVRSFCKVMEKYGIQPVIGLSIRLEMDDGSDMLLYAYAKDDRGYRNLLKISSATSTKE
                     TETLPYKWLQAYSEGCVFICPMTDRSWDDKRSAETIRTIRNACRTLYIGISRPGGVKH
                     QDEDGVLSIADETDTTITAFHESRYIAEEDAFAFEVAAAIRSGYKLNDPSRPKMEIYD
                     AYVPEEQELRQWFSDHPEWIDTMAEIMLSCNAGLPPSQTLMPAFPVPEGHTSASLLKK
                     NCEEGLVRRLGTVSRTYYDRLGAELTVIEDMGFSDYFLIVEDFMRFSKENGILTGPGR
                     GSSAGSLVAYSLGITDVDPLKYGLIFERFLNPGRITMPDIDIDFADNRRSEVIEYVAR
                     KYGKAHVAQIITFGTLSSKSVARNVARVFDFSNEEMSFLSKQIQEGHSKRLEESVNKS
                     KALQDWIALDPVRAKWFQAAKTLEGLPRNASTHAAGVILSPMPLVETVPLQSGGDDIY
                     LTQWAMGDVEEIGLLKMDFLGLRNLTLMDRIQSMVHYDTGKTLRFEDIPLDDAKTFDL
                     FKAGDMTGIFQFESDGMRDALRLIKPDEFSDLYAINALYRPGPMENIPLYSRRKNNKE
                     KIDYLHPQLEPILRETEGIIVYQEQIMQIAVQIAGFTMSEADLLRRAVSKKKREILQQ
                     EREHFVRSAVQHDFPERSASEIYDLIVKFADYGFPKSHAVAYSLISYQLAYVKANAPA
                     YFYAALLSMATGNQEKIMEYIHEIRGKGIEILPPSIQKSKYSHIVERGSIRIGLGAIK
                     GVTPSFYNVVKEARTSESWKTLFDFSAAIGGTNFTEKTIIPLIKAGALDEFGEERSVL
                     LASIEAARNHAQFVSPDGDDLLHEIIYSVAKPKYTPGGSMPRFAMLEYEREVLGFYLS
                     EHPALEMKKSLGEKMVDLVRVPDFKERSMLKVAGMIREIKRIRTKKGEAMAFLTLQDE
                     TGELSCTIFPRQYAASNIHLQELAMVQITGTMEKRNGQTQLIVQQINRV"
     gene            complement(5143..>5685)
                     /locus_tag="VFX34_00840"
     CDS             complement(5143..>5685)
                     /locus_tag="VFX34_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX5563476.1"
                     /translation="GEKHYGWKMSDACARLLFAGIVGDTGRFLFPSATVRTFEIASGL
                     IKYNFDRTKLFAEMYEEDRRLLHLKGFMYQQFTIDENGAAFIKIDKSILEQFNVTASE
                     TSQLVGSLGDVKGICAWVIFVEEDDQIRVRFRSKGPIINQLAAEYDGGGHPLASGASI
                     YSWDKSDEIIAKLKQLCAVN"
BASE COUNT         1535 a         1325 c         1294 g         1531 t
ORIGIN      
        1 aagcaaagat attattaaac attcgatata aggaaattgg ggttcattta ggatagcggt
       61 ttaaaaggga ttcgttgatt ggagcgaaag gcggcgactc ctgccggaat agcatgagct
      121 gaagaccctg gactaagcaa agcgagggaa gcggctgaag ccatgccggc ggaaacatac
      181 taacattagt agcacaaacc aaggccatgg tttgtgctac tattttttat gtagaagtga
      241 agggaagtcg cgcggacgct gggatctttg aatccgtata aggaaaccga ctaccttcac
      301 atccttattt gaatcagttt agtatgttgg gtccttgaga tcgtgtataa gaaaatggaa
      361 tcgatgaggc gctgtgaagg acttctataa gtttatagag ggggatgaaa gatgaaacat
      421 gtaattgctt tcgatgtaag tatgggaaaa agtacaatgg ttatttatga tcacaaccac
      481 tgttgtcggt atgaaggtga attggaacac acggtgtccg gtttcaattc attaaaagag
      541 cgaattaaat tactgagtga attggacggc caggtacctg acatcgtctt cgaggccact
      601 ggtgtctatt caaaagggct tgagggattt ctgcaggcac aacactatcc ttacattcgc
      661 ctgaatccat tggaggctaa gctccagatg gctaccatgc gtcggcataa gacagatatc
      721 agtgatgccc atgaactggc gaaatcccat tttaaatcgg atcggcaaga aacttatgta
      781 caagaggact atttcgaaca gatgcgggca ctggggcgct actatgacga tctagaaaaa
      841 gagattaggc ataactacaa tcgattacac gctttcctac agttaagttt tcctttattt
      901 gaaaaagtgt ttttgaaaaa ctcagccatg tttttaaata tcgttcagct ctatccacac
      961 cccgcgtgtt tacttaagct ttccaaggaa gagctggggc accaaatcaa aagggcaacc
     1021 cgtaagatta tatcaactca aaaagcggag gagaaagcag ttctactgat tgccgctgcg
     1081 aaaaactcat attcggctat tggaccggag gacgtacgtt gcgaacattt aaggcactat
     1141 gcaaagcgta ttgcggacct agtcaatcag aaaaaagata tcatcaaaca gatggtggaa
     1201 ttatcaaagg aaagagtgga tttccaagtg ctcgtgtcat ttccgggaat cggagaaact
     1261 acagccgtaa gagtcattgg cgaattaggt gacattcgac gctttaagaa tccaaaacag
     1321 attaatgcgt acgcagggat tgatattcaa cgacaccaat cgggcaagct ccagtaccag
     1381 gatcggatta ataaacgggg aaatcgaaga ttgaggaaaa tcctttattt catggtgatg
     1441 tcgatgattt ccttgcgagc gaagacaaaa aacacaatcg tggattatta tgatcaattg
     1501 aaaaagcaac ctcagagtaa gcctcataag gttgcggtaa tcgcatgtat gaataagttc
     1561 ctgaaagtcg catttcacct tatccaccat gacgtcctgt atcattacga aacagccaaa
     1621 gtttcgtaac aggatagctt cactatagca caaatgacct tgcgaaaaaa ataaatattc
     1681 gtcaagtcta tttggcatat tcatttttag gagcacctgt tgcttggagt gcaggcgctc
     1741 gactcctgcg ggaatagcat gagccttgag accccgcagg gcgcacgcct ttcgcgaccg
     1801 aggaggctca agccatgccc gcggaaagcg agcgctggaa cggaaagcaa cagtccgttg
     1861 atagaactgt acatctttaa ttcatttaga acctcgaaag aattaataaa gtacttgact
     1921 acatgtaaga aagcgtccgc ctgaagcgta aatcaacaat ttccaagtga atcctaatat
     1981 aaaggacttt tcgacgacct caacaaatag gatgtacatt acgtcgccta ggtgtcagtc
     2041 aggatacctt taatcttcta aaccctgtta atctgctgga cgatcagttg tgtttggccg
     2101 ttgcgctttt ccatcgttcc tgttatttgt accatagcaa gctcttgaag atggatgttg
     2161 cttgcggcgt attgccttgg aaagatggtg cacgatagtt ctcctgtttc gtcttggaga
     2221 gtaaggaaag ccatcgcttc gccttttttc gtccggatcc gtttgatctc tcttatcatt
     2281 ccggcgactt tcagcataga ccgttctttg aaatcaggta cgcgtacgag gtcgaccatt
     2341 ttttcaccaa gcgatttttt catttccaat gccggatgtt cggataaata aaagccaagt
     2401 acttcacgtt catattcgag cattgcaaat cttggcatcg acccgcccgg agtgtatttc
     2461 ggctttgcta ccgaataaat gatttcgtga agcaagtcat ctccgtcggg actcacaaat
     2521 tgggcatgat tgcgggcagc ttcaattgac gcaagaagga cggatcgttc ttccccaaat
     2581 tcatcgagtg ctcctgcttt tatgagagga atgatcgttt tttcggtaaa attcgttcct
     2641 ccgatcgcag ccgaaaagtc aaagagtgtc ttccagcttt cggacgtccg ggcttctttg
     2701 acaacattat aaaaagacgg tgtaacccct ttaatcgccc ccagtccgat tcggattgaa
     2761 cctctctcaa cgatatggga gtatttgctc ttttggatgg atggagggag gatttcaatt
     2821 cctttccctc tgatttcatg gatgtattcc attattttct cttgatttcc agtagccatc
     2881 gataacagtg cggcgtaaaa ataggcgggg gcattcgctt tcacgtatgc caactgatag
     2941 gaaatcagtg aataggcaac cgcatggctc ttcgggaatc cgtaatctgc aaatttaacg
     3001 atcaaatcgt atatttcact agcagaacgt tcggggaaat catgctgtac ggcgcttctt
     3061 acgaaatgct ccctttcctg ctgcagaatc tcacgcttct ttttgctgac agcccttcga
     3121 agcaaatccg cttccgacat cgtaaatccc gcaatttgta cggcgatttg catgatttgc
     3181 tcctggtata caatgatgcc ttctgtttcc cgtaggatcg gctcaagttg aggatgtaaa
     3241 taatcgattt tttccttatt gttttttctt cggctataaa gtgggatatt ctccatcgga
     3301 cccgggcggt ataatgcatt gattgcgtaa agatcgctga attcatccgg tttgattagc
     3361 cttaatgcat ccctcatgcc atccgactcg aattggaaga tccctgtcat gtctccagct
     3421 ttgaacaggt cgaaagtttt cgcatcatcc aacggaatgt cttcgaaccg cagtgtcttg
     3481 cccgtgtcat agtggaccat cgattggatc cggtccatta acgtcaaatt gcgtaatcct
     3541 agaaaatcca ttttcaataa tccgatttct tcgacgtctc ccatcgccca ttgcgtcaag
     3601 taaatatcgt cccccccact ttggagagga accgtttcga ctaacggcat gggagacaga
     3661 atgacgcctg cggcatgcgt agaggcattc ctaggcaagc cttctaatgt tttggccgct
     3721 tgaaaccatt tggcacggac aggatccaag gcaatccagt cttgaagggc tttcgacttg
     3781 ttaacggact cttccaatct tttgctatgt ccttcttgta tctgcttcga taggaaagac
     3841 atctcctcat tggagaagtc gaacacccgt gcaacgttcc gtgcaacaga tttggatgaa
     3901 agtgtgccaa acgtaataat ttgggcaaca tgtgctttgc catatttcct cgctacatat
     3961 tcaatcactt ctgaacggcg gttgtccgca aaatcgatat cgatatcggg catcgtaatc
     4021 ctgcctggat taaggaagcg ctcgaaaatg aggccgtatt tgagtggatc gacatccgtg
     4081 attccgaggg agtatgcgac gagggagccg gctgaggagc cccttcccgg gccagtcaga
     4141 attccattct ccttcgaaaa gcgcatgaaa tcctctacga tgaggaaata atcggagaaa
     4201 cccatgtctt caattacagt cagctctgcc ccgagccgat cgtaataagt ccggctaacg
     4261 gttccaagac gacgaaccaa gccttcttcg caattctttt tcagtaaaga agccgacgta
     4321 tgtccttcag gaaccggaaa agcaggcatt aacgtctggc taggaggcag ccccgcattg
     4381 cacgacaaca tgatttcggc cattgtgtca atccattcgg gatgatcgga aaaccattgc
     4441 cgcaactctt gctcttcagg cacgtaggca tcatagattt ccattttcgg ccgtgatgga
     4501 tcattcagct tgtagccgga cctgattgct gcagccactt caaaagcgaa ggcatcttct
     4561 tcggcaatat atcttgattc atgaaaggct gtaatcgtgg tgtcagtctc atcggcgatg
     4621 ctaagaactc catcctcatc ctggtgcttt acaccaccag gccgtgagat gccaatatag
     4681 agcgtccgac aagcattccg gattgtccgg atcgtttccg cgctcctctt gtcatcccat
     4741 gaacgatcgg tcatcggaca aatgaagaca cacccctcgc tataggcttg cagccattta
     4801 taaggaagtg tttccgtttc ctttgttgac gtcgcactgc taattttaag caggttgcga
     4861 tagcctctgt cgtctttggc gtacgcatag agcaacatat cggaaccgtc atccatctcc
     4921 agccggatcg atagcccgat aacaggttgg atgccgtact tttccatcac tttgcaaaat
     4981 gaacggacgc catacatctt tgaatttacg attccgacag atctagcccc tctcttttgc
     5041 agaagcgggg ctagatggtc aagctttata atgccgcgca gcaagtcggc gccggtcaca
     5101 atttgtggat agacaagtac caatgaaaat acctccgatc cgttaattca ccgcacataa
     5161 ttgtttcaat ttcgcaatga tctcatcgga tttatcccac gaataaatgg aagccccgga
     5221 agcaagcggg tggcccccgc cgtcatattc ggcagcgagc tgattgatga tcggcccttt
     5281 cgaccggaat cggacgcgga tttggtcatc ctcctcgaca aagatgaccc aagcacaaat
     5341 ccccttcaca tctcccaggg atccgacaag ttgggacgtt tcactcgcag ttacgttgaa
     5401 ctgctccaaa atcgatttgt cgattttaat gaaagcggct ccgttttcgt cgattgtgaa
     5461 ttgttgatac atgaagcctt tcaaatggag caatcgacga tcttcctcat acatttccgc
     5521 gaatagcttc gtccggtcga aattgtattt gatcaacccg cttgcaatct caaatgtcct
     5581 cacagtcgca ctcgggaaaa ggaaacggcc cgtatctccc acgattcccg caaagagcag
     5641 acgtgcgcaa gcatccgaca ttttccaacc gtaatgcttt tcccc
//
LOCUS       DASULI010000040         9855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1870584,
            whole genome shotgun sequence.
ACCESSION   DASULI010000040 DASULI010000000
VERSION     DASULI010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870584"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /gene="ligA"
                     /locus_tag="VFX34_00845"
     CDS             <1..1662
                     /gene="ligA"
                     /locus_tag="VFX34_00845"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5563477.1"
                     /translation="GLAVSLKYENGTFVQGSTRGDGTVGEDITANLKTIRTVPLKLAD
                     PVTIEVRGEAYMPKKSFMRLNEARDENGEEPFANPRNAAAGSLRQLDPKIAASRNLAV
                     FIYGVGGDGAAYGQDSHSDSLDYLASIGFETNKKSKRCKTIEDVIDYIESWQERRQDL
                     DYEIDGIVIKVDGYEDQEQLGFTAKSPRWATAYKFPAEEVHTKLLDIELSVGRTGVVT
                     PTAILEPVRVAGTTVARASLHNEDLIREKDIRIGDAVVIRKAGDIIPEVVTVLVEQRS
                     GNEEPYHMPDNCPVCDSELVRIEGEVALRCVNPQCPAQMKEALIHFVSRNAMNIEGIG
                     ERVVDQLYTSGLVHDFSDLYGLTKEQLLSLERMGEKSATNLLTAIEASKSNSLEKLLF
                     GLGIRHVGEKAAHILSEEFGTMDEIAKADAERLTSIHEIGDKVADAVVTFFNNEDVLD
                     VIGKLKSYGVNMNYKGRARQEIPTDGPFAGKTVVLTGKLSVLTRNEAKERIESLGGKV
                     SGSVSKKTDLVIAGEDAGSKLVKAEELGITVWNETELVDALGEKE"
     gene            1668..2783
                     /locus_tag="VFX34_00850"
     CDS             1668..2783
                     /locus_tag="VFX34_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HEX5563478.1"
                     /translation="MKRLAALPIISAVLLLGGCLPSIGPDKEEVIQENDEQIEETVMI
                     PDVQVKDTYYKTLLPLKKSASRGLVVSNIYTKYDIGEAEEGLLRLSAQHFDPKNYYFQ
                     EGQYIDAATARAWLARSSTNEAGLNPPEAEGEDAEENPIYLAHIIEQNYLTLTDDKKV
                     RLSGMSIGLALNSVHYDKAGKETPISDAQLEKQGIAMADQIVSRLRAKEGFQDIPIVI
                     GLFKQEKRNAIVPGTYFATAFADKGKSSASGWKEVNEQYVLFPVPADIDDYRTINEKF
                     SHFKQDIDKYFPSFVNVIGKGYFKENRMQSISIDIPIQFFGKSEVIGFTQFLTGQIIK
                     YFPNIDVEVSVTSVNGPEALIVKKAGSDEPFVHIYEY"
     gene            2896..3183
                     /gene="gatC"
                     /locus_tag="VFX34_00855"
     CDS             2896..3183
                     /gene="gatC"
                     /locus_tag="VFX34_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5563479.1"
                     /translation="MTKFTAEDVQYFANFARIAFSDQEAEEYSTIISELVEFGDVLKE
                     VDTDNVEPMTHPLQLFNVLREDVPQDILDRDEMLASVKEHEDGMIKVPNIL"
     gene            3208..4674
                     /gene="gatA"
                     /locus_tag="VFX34_00860"
     CDS             3208..4674
                     /gene="gatA"
                     /locus_tag="VFX34_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5563480.1"
                     /translation="MTLMKKTAAELQALLHNGEVSVKEVTEDSFKQIAALDGQVKAFL
                     ALTEEEALAKAESLDNTPKEGRGPLFGLPIGIKDNIITKGTATTCASKMLEDFVPVYN
                     ATAVEKVNETGMISVGKLNMDEFAMGSTTERSAFQTTHNPWNLDCVPGGSSGGSAAAV
                     AAGEVPFSLGTDTGGSVRQPAAFCGVVGMKPTYGRVSRNGLVAFASSLDQIGPITRTV
                     EDNALLLNAISGVDHRDSSSSPQQVPDFKAALTGDIKGLRIAVPKEYLGEGIAEDVKD
                     KVRASLKVLESLGASIGEVTLPHSKYASPTYYAIATAEASSNLARFDGIRYGFRAEGA
                     KNLDEIYLRSRAEGFGTEVKKRILYGTYALSADHHEELYVKAMKVRTLISQDFGSVFD
                     NYDVIVGPTSATTAYEIGGAVKDQVTLYANDILTVSVNLAGIPAISIPCGFSDGLPVG
                     LQIIGKHFDEETLYRVAHAFEQATDFHKRTPSIGEGTN"
     gene            4674..6104
                     /gene="gatB"
                     /locus_tag="VFX34_00865"
     CDS             4674..6104
                     /gene="gatB"
                     /locus_tag="VFX34_00865"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5563481.1"
                     /translation="MNFETIVGLEVHVELKTDTKIFSPSPSHYGAEPNMNIHVKDLAY
                     PGTLPTLNKRAVDFGMKASMALNCDVADVMNFDRKHYFYPDNPAAYQISQDKRPVGQN
                     GWIEIEVEGKKKKIRIERVHLEEDAGKLTHSDNGYSLVDLNRQGTPLIEIVTEADIRS
                     PEEAYAFLEKLKAIIQYTGVSDVRMEEGSLRCDANISLRPFGQDKFGTKTELKNLNSF
                     NFVRRGIANEVERQEKILLSGGEIQQETRRYDEATGQTVLMRVKGSANDYRFINDPDL
                     PEIHIDQEWKDRVRAEIPELPDARKARYVNDLDLPEYDAHVLTLTKEMSDFFDATVAA
                     GADAKLASNWLMGEVSAYLNAEQKELHDTALTPDGLSSMIKLIADGTISSKIAKKVFK
                     ELIENGGNAADIVKEKGLVQISDEGTLRAIVTEVLDANEQSIEDYKNGKDRAVGFLVG
                     QVMKATKGQANPPMVNKILLEEINKR"
     gene            6346..6915
                     /locus_tag="VFX34_00870"
     CDS             6346..6915
                     /locus_tag="VFX34_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5563482.1"
                     /translation="MKTEKQYFDEAISLADYMDRMEKHKENSFGIYEKFDVPQDDAFI
                     EIAKTEKPNILVITEDWCGDAMMNNPILRRIAEAADLDVRTVYRDEDTDLIDRYLTNG
                     GRSIPIYLLLSQDGEVLAKWGPRAASIQEDVMERRKQLPAKDAPDFEERQRAFISELM
                     KEYTTQPELWLTVYEDIRNTFMPALQQTK"
     gene            7026..7973
                     /locus_tag="VFX34_00875"
     CDS             7026..7973
                     /locus_tag="VFX34_00875"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX5563483.1"
                     /translation="MKRARIIYNPTSGREIFRRHLADVLEKLEMAGYETSVHATTGEG
                     DATEAAKHAVERGFDVIIASGGDGTLNEVVAGVSPFEKRPTIGLIPTGTTNDFARALR
                     IPRDIDEAIDIIVRGETIPVDVGLMNDRHFINIAGGGRMTELTYEVPSKLKTVLGQLA
                     YYLKGIEMLPSIHSSNVKIEYDNEVFEGEVMLFLVGLTNSVGGLEKLAPNSSVNDGRF
                     TLLVLKKCNIAEFIRVVSLALRGEHLDDPLVISAQPKKVKITSTEEVLLNLDGEYGGT
                     LPATFENRYRHIEMFAPLDQLREQDRIENDQENEEAALD"
     gene            complement(8048..8482)
                     /locus_tag="VFX34_00880"
     CDS             complement(8048..8482)
                     /locus_tag="VFX34_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5563484.1"
                     /translation="MEIKRKVLAYITKDEHAERKLLVFEQKDHPDAGLQVPGGTIEED
                     ELLIDALYREIEEETGIRREQLQLNGKVHKENYFPENRKDVIHERNIFHLTYMGGDEN
                     EWDTCVNSTGKDNGMIFHCRWVSVNDLPALAAGQDEAIEFIN"
     gene            8785..>9855
                     /locus_tag="VFX34_00885"
     CDS             8785..>9855
                     /locus_tag="VFX34_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5563485.1"
                     /translation="MGNEEAVFYSRVLTELTLSFHIIYATIGVGVPLMIMIAQWVGIK
                     KNDEHYILLARRWARGFVITVAVGVVTGTAIGLQLSLLWPNFMEFAGNVIALPLFMET
                     FAFFFEAIFLGIYLYTWDRFENQKKHLLLLVPVAIGASFSAVFITMVNAFMNAPQGFD
                     IVNGELVNINPIIAMFNPAMPTKVAHVVITAYMTAAFVLAAIAAFRLLKGSNHVYHKK
                     ALYLTMKLGFVFSLATALIGDFSGKYLAEYQPEKLAAAEWHFETQENAPLILLGVLND
                     GEVKYAIEIPFGLSMLAHNNPKAEVIGLDQFPEDEIPPLYIHYLFNIMVFIGMWMTIL
                     SALYWIGVNRGWKRVSSRWFRWL"
BASE COUNT         2724 a         2228 c         2594 g         2309 t
ORIGIN      
        1 ggactagccg tttcattgaa atatgaaaac gggacgttcg tccaaggatc gacgcgcggg
       61 gatgggacgg taggtgagga tatcactgcg aacctgaaga cgatccggac tgttccgctg
      121 aagcttgctg acccggttac aatcgaggtt cgcggcgaag cgtatatgcc gaaaaaatca
      181 ttcatgcggt tgaatgaagc acgcgatgaa aacggtgaag agccgttcgc aaatccacgg
      241 aatgcggcag cgggttcact tcggcagctg gatccgaaaa tagccgcaag ccgcaatctc
      301 gccgtattca tttatggcgt tgggggggat ggtgccgcct acggtcagga cagccattcc
      361 gattcgctcg attatctagc gtcaatcggc ttcgagacga ataagaagag caagcgatgc
      421 aaaacgatcg aagacgtgat tgactatatc gagtcctggc aggaacgcag gcaggatttg
      481 gactatgaaa tcgatggaat cgtcattaaa gtggacggct atgaagatca ggagcagctt
      541 ggcttcacgg cgaaaagtcc gcgttgggcg accgcctata aattccccgc ggaagaagtg
      601 cacacgaagc ttcttgatat cgagctgagc gtcggcagga cgggcgtcgt cacgccgact
      661 gccattttgg agccggtccg tgttgccggg acgacagtgg caagggcctc cctccataac
      721 gaggatctca tccgcgaaaa ggatatccgt atcggggacg cagtcgtcat ccgaaaagcg
      781 ggggacatca ttccggaggt tgtcaccgta ttggtcgagc aacggtccgg gaacgaagag
      841 ccgtatcata tgcccgacaa ttgcccggtt tgcgattccg aacttgtcag gatcgaaggg
      901 gaagtggcgc ttcgctgcgt caatccgcaa tgccctgcgc aaatgaagga agcgctcatc
      961 cattttgtct ctcgcaatgc gatgaacatc gaagggatcg gcgaacgtgt cgtggaccaa
     1021 ttgtatacat ccggccttgt ccatgacttt tcagatcttt acggcttgac gaaggaacag
     1081 ctgttgtcgc ttgagcggat gggagagaag tccgcgacga atttattgac agccatcgaa
     1141 gcatcgaaat ccaattcgtt ggagaagctg ctgttcggcc ttggcatccg gcatgtcggg
     1201 gaaaaggctg cacacatatt atccgaggaa ttcggtacga tggacgaaat cgcaaaggcg
     1261 gacgcggagc ggcttacatc gatccatgaa atcggcgaca aagtggcgga tgcagtcgtt
     1321 acatttttca acaatgaaga tgtattggat gtaatcggaa agttgaaatc ttacggcgtc
     1381 aatatgaact ataagggaag agcgcgacag gagattccga cagatggccc gttcgcaggt
     1441 aaaactgttg tgttgacggg caaactctcc gtgctgacgc gcaatgaagc aaaagagcga
     1501 atcgagtcgc tcggcgggaa agtgagcggg agtgtcagca aaaagacgga tctcgtcatt
     1561 gccggggaag atgccggatc gaagcttgtg aaggcggagg aacttggaat aacggtatgg
     1621 aacgaaacag agttggtcga cgcgctcggc gaaaaggagt aaattgcatg aaaaggctag
     1681 cagcattacc catcatatca gccgtccttt tgttgggcgg ctgtctaccg tccatcggac
     1741 ccgacaaaga ggaagtcatc caggaaaatg atgagcagat cgaagagacc gtcatgattc
     1801 cggatgtaca agtaaaggac acctattata agacacttct cccgcttaag aagagcgcaa
     1861 gccggggcct tgtcgtaagc aatatttata cgaaatacga tatcggggaa gctgaggaag
     1921 ggctcttgcg tttatccgcc cagcacttcg atcccaaaaa ctattacttt caggaaggcc
     1981 agtacatcga tgcagcaacg gcacgcgcat ggcttgccag aagttcgacg aatgaggctg
     2041 gcttgaatcc ccctgaagcg gaaggggagg acgcggaaga gaatccgatc tatttggcgc
     2101 atattatcga acaaaactat ttgacgttaa cggacgataa gaaagtgcgt ctctccggta
     2161 tgtcgatcgg actcgccttg aattccgtcc actatgacaa agctgggaaa gagacgccga
     2221 tctctgacgc gcaacttgaa aagcaaggca tcgccatggc ggatcaaatt gtcagcagac
     2281 tgcgggcgaa agaggggttc caagatattc cgattgtcat cggtctcttc aaacaggaga
     2341 aaaggaatgc aatcgtacca gggacatatt tcgctaccgc ttttgccgat aaaggaaaat
     2401 catccgcttc cggatggaaa gaagtgaatg agcaatacgt gttgtttccc gttccggcag
     2461 atatcgacga ttaccgtacc atcaatgaga agttcagcca ttttaagcaa gatatcgata
     2521 aatatttccc aagcttcgtc aatgtgatcg ggaaaggtta tttcaaagaa aaccggatgc
     2581 aatccatttc catcgacata ccgatacagt tttttgggaa gagtgaagtc atcggtttca
     2641 cacagttttt gactggtcaa atcatcaagt atttcccgaa tatcgatgtc gaagtgagcg
     2701 tcacttcggt gaacgggcca gaggcgctaa tcgtcaagaa ggctggtagt gacgaaccgt
     2761 tcgttcatat ttatgaatat tgaatgaagt ccgaacgcgc ttccgtgcca tcgtgcacgg
     2821 gatgcgcagt tgcttttcaa cataccgcta aggtggtatg attagtcgta caattgcagt
     2881 ttgggaggaa atcaaatgac gaaatttacg gcagaagacg tgcaatactt cgcgaatttt
     2941 gcaagaatcg ccttttcaga tcaggaggcg gaggaatatt cgacaatcat ttccgagctt
     3001 gtcgaatttg gagatgtgtt gaaagaagtc gatacggaca atgtggagcc gatgactcat
     3061 ccgttacagc ttttcaatgt gctccgggaa gatgttccgc aagatattct cgatcgggac
     3121 gaaatgttgg cgagcgtaaa agagcatgaa gacggaatga tcaaagttcc gaatatcctg
     3181 tgataacggt ttttaggagg aagtaagatg acgttaatga aaaagacggc agcagaactt
     3241 caagcacttt tacataacgg ggaagtctct gtcaaagaag tgaccgaaga ttcatttaag
     3301 cagattgctg cgctggacgg tcaagtgaag gcattcctgg cgttgacgga agaagaagcg
     3361 ttggcaaaag cggaaagctt ggacaatacg ccgaaagagg gccgcgggcc gctattcgga
     3421 ttaccgatcg gcatcaagga caatatcatc acaaaaggaa cagctacgac atgcgcaagc
     3481 aaaatgcttg aggatttcgt tcctgtctat aacgcaactg ccgtagaaaa agtgaacgaa
     3541 acgggcatga tcagtgtcgg taaattgaac atggacgagt tcgccatggg ttccacaact
     3601 gaacgttcag cattccagac aacgcataat ccatggaatt tggactgcgt ccctggcggt
     3661 tcatccggcg gttcagcagc tgcagtagca gcgggggaag tgccattctc gctcggtacg
     3721 gatacaggcg gatccgtccg tcagccggct gcattctgtg gtgttgtcgg gatgaaaccg
     3781 acctacggca gagtgtcgcg caacggactt gtcgcgtttg cttcctcttt ggatcaaatc
     3841 ggcccgatta cgcggacggt cgaggataat gcactattat tgaatgccat ttcaggtgtc
     3901 gatcatagag actcctcctc ttcgccgcag caagtccctg atttcaaagc tgctttgacg
     3961 ggcgatatca aaggcttgcg aatcgcagtg ccgaaggagt acttgggaga aggcatcgca
     4021 gaggacgtta aagataaggt ccgtgcctca ctgaaagtcc ttgaatcact cggcgcatca
     4081 attggggaag tgacgcttcc gcattccaaa tacgcgtcgc caacgtacta tgcgattgca
     4141 accgctgaag catcttccaa ccttgcgaga ttcgatggaa tccgttatgg cttccgtgca
     4201 gaaggcgcga aaaacttgga cgaaatctat ttacgttcac gtgctgaagg attcggtaca
     4261 gaagtgaaaa aacggatcct ctacggtacg tatgcactaa gcgcagatca tcatgaagaa
     4321 ctgtatgtaa aggcgatgaa agtacgtacg ctcatttcac aggactttgg atcagtcttt
     4381 gataattatg atgtcatcgt cggaccgaca agcgcgacga cagcatacga aatcgggggt
     4441 gccgtgaagg accaagtgac attgtatgcg aatgacattt tgacagtgtc cgtcaacttg
     4501 gcaggtattc cggcaatttc aattccatgc ggcttttcgg atggattgcc ggtcggactc
     4561 caaatcatcg ggaaacattt cgatgaagag acactataca gagtggctca cgcgtttgaa
     4621 caggcgaccg atttccataa acgcactcca tctatcgggg agggtactaa ctaatgaact
     4681 ttgaaacgat tgtcggactt gaagtccacg ttgaattgaa aactgataca aaaatcttct
     4741 caccgtctcc atcccattac ggtgcagagc cgaatatgaa catccatgtg aaggacctgg
     4801 catacccagg gacgcttccg acgttgaata aacgcgcggt ggacttcggg atgaaagcat
     4861 ccatggcact aaactgtgac gttgccgacg tcatgaattt cgaccggaaa cattacttct
     4921 atccggataa cccggcagcc tatcaaattt cccaagacaa gcgtccggtc ggccaaaacg
     4981 gctggatcga gatcgaagtc gaagggaaaa agaagaaaat ccgcatcgag cgcgtccatc
     5041 ttgaagagga tgcaggcaaa ttgacgcact ccgacaacgg ctattcactt gtcgatttga
     5101 accgtcaagg gacgcctttg attgaaatcg tcacggaagc ggatatccgc tctccggaag
     5161 aggcgtatgc attccttgaa aaactgaaag cgatcattca atatacaggc gtttccgatg
     5221 tccgtatgga ggaaggttcc cttcgctgcg atgcgaatat ttcattgcgc ccgttcggac
     5281 aagataaatt cgggacgaaa accgaattga aaaacttgaa ctccttcaac ttcgtccgca
     5341 gagggattgc gaatgaagtc gagcgccagg aaaagatcct tctctcggga ggagaaatcc
     5401 agcaggaaac acgccgctat gacgaagcga cgggccaaac tgtgctcatg cgcgtaaaag
     5461 gcagtgcgaa cgactatcgt ttcatcaacg atccggatct ccctgaaatc catatcgacc
     5521 aagaatggaa ggaccgtgtc cgtgctgaaa ttccggaact tccggatgcg cgtaaagcaa
     5581 gatatgtcaa tgatctcgat ctgccggaat atgacgcgca tgtcttgacg ttgacgaaag
     5641 agatgtccga cttcttcgac gcgaccgttg ctgcaggcgc agacgcgaaa cttgcttcca
     5701 actggttgat gggcgaagtg tccgcttatt tgaatgctga acagaaagag cttcatgata
     5761 cagcactcac gccggacggc ctctccagca tgatcaaatt gatcgcggat ggcaccattt
     5821 catccaaaat cgcgaagaaa gtgttcaagg aactgattga aaacggtgga aacgccgctg
     5881 acatcgtcaa agagaaaggc ctcgtccaaa tctccgacga aggcacactc cgcgcaatcg
     5941 tgacagaagt gctcgatgcg aatgaacaat cgatcgaaga ctacaaaaac ggcaaggacc
     6001 gcgcagtcgg cttcctcgtc ggacaagtga tgaaagcgac aaaaggacaa gccaatccgc
     6061 caatggtgaa taaaatcctc cttgaggaaa tcaacaagcg ataagtccga aagtgaaacg
     6121 tccgatacgg gtcttgagaa agtccccgta ttgggcgttt taccgttttt ccacgtccag
     6181 ctccgggcgc tagggtgcct taatttctgc aaaacccgca gaaatacggc aaatcgaacc
     6241 ctacgctgtt cgattcgatt ttgtttagca tcctatttga ttcacacaac gaatagcctt
     6301 acacttaaga aaaagggttc tattttataa aaggaaggtg tcggaatgaa aactgaaaag
     6361 cagtatttcg atgaagcgat ctcgcttgct gactacatgg atcggatgga aaagcataaa
     6421 gaaaacagct tcggcattta tgaaaaattc gacgtgccgc aagacgatgc atttattgaa
     6481 atagcaaaaa cggaaaagcc caatatacta gtcataacgg aagattggtg cggcgatgcc
     6541 atgatgaaca atccgattct gcgccgaatc gcggaagccg ctgatttgga cgtgcggaca
     6601 gtctatcgtg acgaagatac agatctgatc gacaggtatt tgacaaacgg cggccgctcg
     6661 atcccgatat acttgctgct cagccaagat ggcgaagtgc tggcgaaatg gggtccgcgc
     6721 gcagcatcca tccaggaaga cgtcatggag cgccgcaaac agctgccagc gaaagatgca
     6781 cccgattttg aagaaagaca gcgggcattc atttctgaat tgatgaagga atatacgaca
     6841 cagcccgaac tatggctgac cgtatacgag gacatccgca atacattcat gcctgcattg
     6901 caacagacga agtgaaacaa taatgggaac tttttagcag gacggacagt ccaagtaagc
     6961 gtctgatagt tggaacatcg acgcatgcaa gcgttgcatt ttgtatgaat tgagagggaa
     7021 catgtatgaa gcgtgcacga atcatttata acccgacatc aggacgtgaa atattccgga
     7081 ggcatctggc tgatgttctg gagaagctgg aaatggccgg ctatgaaaca tccgtacatg
     7141 cgacaacggg ggaaggcgat gcaaccgaag cggcgaaaca tgctgtggaa cggggatttg
     7201 acgtcatcat cgcgtcaggc ggggatggaa cgttgaacga ggttgtcgcc ggggtatctc
     7261 catttgaaaa aagaccgaca atcgggctca ttccgaccgg gacaacgaat gacttcgccc
     7321 gggcattgcg gattccgagg gatatcgacg aggcgatcga catcatcgtc cggggagaga
     7381 cgattccggt ggatgtcggc ctcatgaacg accgccattt catcaacatc gctggcgggg
     7441 gacggatgac cgagctgacg tatgaggtgc cgagcaagct gaagacggtt cttggccagc
     7501 tcgcctatta cttgaaaggg attgaaatgc tcccttccat ccattcgagt aacgtgaaaa
     7561 ttgagtatga taatgaagtg ttcgaagggg aagtgatgct tttcctcgtc ggcttgacca
     7621 attccgtcgg agggctggag aaattggcgc cgaattcaag cgtcaatgat gggagattca
     7681 cgttattggt tttgaaaaaa tgcaatatcg ccgagttcat cagagtcgtc tcgttggcgt
     7741 tgcgcgggga acatttggat gacccgctcg tcatctctgc acagccgaaa aaagtgaaaa
     7801 tcacttcaac tgaggaagtg ctattgaact tggatgggga atacggcggc acccttcccg
     7861 ccacttttga aaaccggtat cgccatatcg aaatgttcgc cccgctcgac caattgcggg
     7921 agcaggatag aattgaaaat gatcaagaaa atgaagaagc tgcattggat tgaacatcca
     7981 tgcagctttt taatttgcca aatctaacac aagggctcca ttattcgatt aagggctcaa
     8041 ttctccctta gttaataaat tcaatcgcct catcttgtcc cgcagcaagc gcaggcaagt
     8101 cattcacgga aacccagcgg caatggaaga tcatgccgtt gtccttgccg gtactgttga
     8161 cacaagtatc ccattcattt tcatccccgc ccatataggt gagatgaaag atattccgtt
     8221 catggatgac atctttccga ttttcaggaa agtaattctc cttgtgcact ttgccattca
     8281 actgcaactg ttcacgacgg atgccggttt cttcttcaat ttcgcgatag agcgcatcga
     8341 tgagcaattc atcctcttca atggttccgc ccggcacttg caacccagca tcgggatgat
     8401 ccttttgctc gaacacaagg agtttccgtt cggcatgttc gtcctttgta atgtaagcta
     8461 agacttttct tttgatttcc attgtgttca ttcacctctc tttaatctct ctattataca
     8521 taacattcca aatactctca agagcaaaac acccgttgaa aacatttcac attatgtgtg
     8581 acaatcctgt tatttcaatc gtgaaaaggg aaaagtatag agcagttatc ttatgatagg
     8641 aaagagaaga ggaattcaaa ggacggaggg attccgtgta taagaaacaa gagaaatggc
     8701 tcatgtggct cgtctttgtc gtggcgggaa tcatgctgct ttctatttta ggaagaattt
     8761 ttggaagttg aaggagggaa atagatggga aatgaagaag ccgtttttta ttcgcgtgtc
     8821 ttaacggaac tgacgttatc gttccatatc atttacgcga caatcggcgt tggtgtcccg
     8881 ctcatgatca tgatcgcgca gtgggtagga atcaagaaaa acgatgaaca ttatattttg
     8941 cttgccagac gttgggcgcg aggtttcgtc attacagtgg cggtcggggt tgttaccgga
     9001 accgctatcg gtcttcagtt gtcgttacta tggccgaatt ttatggagtt cgcgggaaat
     9061 gtcatagcgc ttccattatt tatggaaacg ttcgctttct tttttgaagc catcttcctc
     9121 gggatttatt tatacacatg ggatcggttc gaaaatcaga aaaagcactt gctgctatta
     9181 gtgccggttg cgatcggcgc ttcgttttct gctgtattca ttacgatggt gaatgcgttc
     9241 atgaatgctc cacaaggctt cgatatcgtg aatggggaac tcgtcaacat caatccgatc
     9301 atcgcgatgt tcaacccggc gatgccgacg aaagtggcgc atgtcgtcat cacggcgtat
     9361 atgaccgcgg cgtttgtgct ggcagccatt gcagcattca ggctattgaa aggctccaac
     9421 cacgtttacc ataaaaaagc gctttattta acaatgaaat tgggatttgt tttctccctt
     9481 gcaacggctt taataggaga tttctccggt aaatatttag cggagtacca accggaaaaa
     9541 cttgccgcag cagaatggca ttttgaaacg caggaaaatg caccgctcat cctgctcggt
     9601 gtcttgaacg acggagaagt gaagtatgcc attgagattc ctttcgggct ttccatgctt
     9661 gcgcataata atccgaaagc ggaagtcatc ggtctggacc aattcccgga ggatgaaata
     9721 ccgccgctct atatccatta tttattcaac atcatggtgt tcattggcat gtggatgaca
     9781 atcctctccg cattgtattg gatcggcgtc aaccgtggtt ggaaacgcgt gtcgtccagg
     9841 tggttccgtt ggctg
//
LOCUS       DASULI010000041         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_947156, whole
            genome shotgun sequence.
ACCESSION   DASULI010000041 DASULI010000000
VERSION     DASULI010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947156"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFX34_00890"
     CDS             complement(<1..527)
                     /locus_tag="VFX34_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5563486.1"
                     /translation="MGIHVENQQVVIKSDVLLKGTLTLPSSKEDLLPAILILAGSGKL
                     NRDANAEKGKFQFNIYKELAEHFSTMGFATLRYDKRGVGESEGNFEKTGLWDAVTDAA
                     NALEFLASHPDIDPQRLIVAGHSEGCIVGTALYAKRPFHGLVLVSGGGGGLRESLDLQ
                     RQQLYSELKHAKGLQG"
     gene            complement(600..1526)
                     /locus_tag="VFX34_00895"
     CDS             complement(600..1526)
                     /locus_tag="VFX34_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX5563487.1"
                     /translation="MFIGSIFLNVIVPILILVVLGAGLQRKFAFNLKAVSNLITYCLM
                     PAAVFINLYETEIDIQVLLEIIGYLVAFSLVIIIVTTTLSKVLKLDRGEAAIFKNSIS
                     LINSGNYGIPVSQLLFHANPLGISIQIIVMVFQNVLTYTYGLYNLISATKSGLEILKS
                     LLKMPVIHAMLLGGLLNWLHVPIPNFVWVPISHLSDAFLAIALILLGAQLAQIELKTI
                     VNRTIVTSSIGRLIIGPTVALLLIFVFGLDGVVAQSLFIASSFPTSRNSSTLALEYDV
                     NPDLAAQTVLFSTIASAITVTFVVYLSGILFV"
     gene            complement(1719..2126)
                     /locus_tag="VFX34_00900"
     CDS             complement(1719..2126)
                     /locus_tag="VFX34_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5563488.1"
                     /translation="MESFIKRIETIYLPVANPKASAEWYEEHLKLKTVGPVEPDAAQA
                     QLALASGQFIFLIQSKERINANFTEIGGEEQCMLTLEVTDFHDLYKVLQEKGATITVI
                     DDNRECGGNFYLYDPDGNKIDIWSGWPAVLKSN"
     gene            complement(2238..>2946)
                     /locus_tag="VFX34_00905"
     CDS             complement(2238..>2946)
                     /locus_tag="VFX34_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX5563489.1"
                     /translation="LHKSFDQLEVLKGIDIDINPGEVVVLVGVSGSGKSTLLRCLNFL
                     EVAEKGTVSIDGRPVDRKKDDLSKVRAEVGMVFQHFNLFPHKTVLENIMEAPLIVKKE
                     DKGKAKQLALALLDKVGLSEKAEVYPNKLSGGQKQRVAIARALAMEPKALLFDEPTSA
                     LDPELVGEVLQVMQDLANDGMTMVVVTHEMKFAKEVADRIIMLDEGCIIEDADPDTFF
                     NHSTNERTRQFLEMVDL"
BASE COUNT          775 a          754 c          604 g          813 t
ORIGIN      
        1 ccttgcaaac ctttcgcatg tttcaactcg gagtataatt gctgcctttg aagatccaga
       61 ctttcacgta aacccccgcc accgccggac actaaaacca atccatgaaa cggtcttttc
      121 gcgtacagtg cagtcccgac aatacatccc tcactatggc ctgccacaat gagccgctgt
      181 ggatcgatgt cagggtggga tgccaaaaat tcaagagcgt ttgccgcatc cgtcaccgcg
      241 tcccaaagcc cagtcttttc aaaattccct tcactttctc caacgccccg tttgtcatat
      301 cgaagcgttg caaaccccat ggtcgaaaag tgctcagcca gttccttgta tatattgaac
      361 tggaatttcc ctttttccgc gttcgcatcc cggttcagtt ttcctgatcc cgcgagtatt
      421 agaatcgctg gaagcaaatc ttctttactt gaaggcaagg tcagcgttcc ttttaataaa
      481 acatcgcttt taatgaccac ctgttgattc tccacatgga tccccatccg cttctcccct
      541 ttttatgcat cataactctt tagtttatga ttcggtggat tcctcctatt ccccttcatt
      601 caaacaaaca aaattccaga taaatacacg acaaatgtta ctgtaatcgc gcttgctatc
      661 gtcgaaaaca gaaccgtctg cgctgccagg tccggattca catcatattc caacgcaagt
      721 gtcgaactat ttcttgacgt cgggaaggaa ctcgcgatga agagcgattg ggcgacaacg
      781 ccatccagcc cgaaaacgaa aatgagaagc aatgcaacag ttgggccgat gataagtctt
      841 ccgatgctac tggtaacaat cgtccgattt acaatcgttt tcaactctat ttgcgcgagc
      901 tgtgccccca ataaaataag cgcgatcgcc aaaaatgcat cggatagatg actgatcggc
      961 acccagacga aattcggaat cgggacgtgc agccaattca acaagccgcc gaggagcatc
     1021 gcgtggatga cgggcatttt caatagcgat ttcaggattt ccaaaccgga ctttgtcgcg
     1081 gatatcaaat tatataagcc atatgtatag gttaaaacgt tttgaaagac catcacgata
     1141 atttggatgg agatgccgag aggatttgca tggaatagca gctggctgac cggtattcca
     1201 taattccccg aattgattaa cgaaatgctg tttttgaata tcgcggcttc ccctctgtct
     1261 aacttcaata ctttactcaa tgtcgttgtg acaatgatga tcactaaact gaaggcgact
     1321 aggtaaccga tgatttccag caggacctga atatcgatct ctgtctcata aagattgatg
     1381 aaaacggccg ccggcataag acaatatgta attaagttcg aaaccgcttt taaattgaat
     1441 gcgaatttcc tttgtaaacc tgcaccaagg actacgagta ttaagatggg tacgattaca
     1501 tttaagaaaa tggatcctat gaacacgtcg tcctccccct aaatcatttt aaagaaatct
     1561 cttccagttg agtatacagg aaagggattc gttcttgaaa ttacgaaaaa gttataacgt
     1621 aacataagaa aaggacttcc cttgactcgg aagtcctttc tttatgcttt gttttttcgc
     1681 tggatagata tcggtcactt taaaagctgc tcttatagtt agttagattt caggacggca
     1741 ggccagccgc tccaaatgtc gattttattg ccatcggggt catacaaata gaagttcccg
     1801 ccgcattctc ggttgtcgtc aatcaccgtt atcgtcgcgc ccttctcctg cagtacttta
     1861 taaagatcat gaaaatcagt aacctccaat gtaagcatac attgttcctc gccaccgatt
     1921 tccgtgaaat ttgcgttgat ccgttccttg ctttggatta aaaagatgaa ttggcccgat
     1981 gctaaagcga gttgggcttg agcagcatcc ggttcaaccg gaccgaccgt ttttaatttc
     2041 aaatgctcct cataccactc tgcagatgcc ttcggattgg caaccggcaa atagatcgtt
     2101 tcaattcgtt tgatgaaact ttccatcgtt ctcccctcct ctgattatat gttatattcg
     2161 acggggagcg aaatatccct gctatttgga aatcagaaat agaaaagcag cctcccagac
     2221 aggaagctgc ttcttcatta caaatccacc atctctaaaa actgtcgtgt ccgttcattc
     2281 gtggagtggt tgaaaaatgt atccggatct gcatcttcga tgatgcaccc ttcgtccagc
     2341 atgatgatcc ggtccgccac ttctttcgcg aatttcatct cgtgtgtgac aacgaccatc
     2401 gtcataccgt cattggcgag gtcttgcata acttgcagca cttcgccgac gagctccgga
     2461 tcgagcgcgg atgtaggttc gtcaaacaga agcgctttcg gttccatggc gagcgcacgc
     2521 gcaatggcga cgcgctgctt ctgtccgccc gataacttat tcggatagac ttccgccttt
     2581 tcggaaagac cgactttgtc caacaaggcg agcgccaatt gcttcgcctt ccccttatct
     2641 tccttcttca caatcagcgg tgcttccatg atgttttcaa gcaccgtctt atgcggaaac
     2701 aagttgaaat gctggaacac catgccgact tctgctcgga ctttcgataa gtcgtccttt
     2761 ttgcggtcaa ccggacggcc gtcaatcgat accgtcccct tctccgccac ttcaaggaaa
     2821 ttcaggcagc gcagcaatgt acttttccca gatccgctga cgccgactaa aacgacaact
     2881 tcaccaggat tgatgtcgat atcgatccct ttcaatactt ccaattgatc gaatgactta
     2941 tggaga
//
LOCUS       DASULI010000042         4486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_714359, whole
            genome shotgun sequence.
ACCESSION   DASULI010000042 DASULI010000000
VERSION     DASULI010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714359"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFX34_00910"
     CDS             complement(<1..557)
                     /locus_tag="VFX34_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019376044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5563490.1"
                     /translation="MSKTTVIYKQQDNFELKADFYPASEASRPVIVYIHGGGLLWGSR
                     EDMKPAQIDFYHQAGFNIFSIDYRLAPESKLPAIREDIVDALDWIGNEGTKQFDYDPE
                     KIAVIGSSAGGFLALLTGTFPNKPKAIVSFYGYGDITGDWAVKPSPHYAAMTNVPREL
                     AKMLVSNEIISVGPIEKRYALYMHA"
     gene            668..2326
                     /locus_tag="VFX34_00915"
     CDS             668..2326
                     /locus_tag="VFX34_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003337433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX5563491.1"
                     /translation="MEFKVMDLLALESMKDAEVLSGGNYIHNVIEGVTIMEGPDIANW
                     IKGGEVILTSLYSIRNFSENEQKDFVSKLAEKRVSALVIKEHSEEISTQLLEAGERNR
                     MPIIQLPQEVPFVDVMYPIMGELFNKQVTKLQYYKEIHDRFTALSLADKGLEKIIHTL
                     EELIGNPVALFDRNFSCIVSTYPELANFEMVEKVHFYEQTSGIKFPHYRQIVKYPDLD
                     NIKGHQIVVPIEALNHNKMYLLIGEIHKSLGELDLIAVENAATSLSLELVKQFAVAEV
                     EKKYKNDLIEELISGRIQSIQAVYEKANVIGWDFNGSFAAVLFKINRQTESMLKQKGD
                     LADRSHFLVSEAIHHYLPNGIITNKSNLFIVLWKIEKTSKNDNAWMNQIKRTALTIQT
                     VVNKQVNDIDVQVGIGSAVSDITEIPKSYREAHDALDLGETLNGLSSITAFSELGIFR
                     LLRHVKDSSALLQFIPKSLKELLDYQQANQSDLLETLQTFLECNQNATQTAKHLFVHY
                     KTVMYRLERIKEITGMDFDDSEEMLSVRVGLKIYELIERENRQY"
     gene            2582..3826
                     /gene="allC"
                     /locus_tag="VFX34_00920"
     CDS             2582..3826
                     /gene="allC"
                     /locus_tag="VFX34_00920"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HEX5563492.1"
                     /translation="MKDIVQKLDLADETAGRLDWLGEFGKDPEGGVSRLLYTKEWVEG
                     QHALKEWMESEGLDARFDEIGNLYGTLKGKNPTETVLTGSHVDTVTNGGRYDGAYGIV
                     AGIIALKYLKKHFGEPVRNIEVVSMAEEEGSRFPYSFWGSKNIVGLAKREDVESIKDF
                     DGVPFVEAMKNAGFDFKDNAEGRKDLKAFVEVHVEQGSVLETENTAIGVVNNIVGQRR
                     YTVEITGQANHAGTTPMGYRKDALYASSHMIHEIISMARKVGDPLVTTVGKIEAYPNI
                     VNVVPGKAIFTIDVRHTDKEAIVSFTEQMTARMQDIAKEHEVEMNIDLWMDEDPVPMD
                     QTVVELIEKQCKVNGLNYKLMHSGAGHDSQIFAPVVPTAMLFVPSLKGISHNPAEFTE
                     PSDLAEGVKALIGALYELGYKE"
     gene            3886..>4486
                     /gene="allE"
                     /locus_tag="VFX34_00925"
     CDS             3886..>4486
                     /gene="allE"
                     /locus_tag="VFX34_00925"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HEX5563493.1"
                     /translation="MGYPKDILSSRSIIKPGQYALIAPEGLVNNVIPGFENCRISILA
                     SPKLGAGFVDYMVTMHEGGKNSEGFCGEEDVQCFVYVISGKVKAKADDQEFILEESGY
                     LYCPPGVTMYLENMESGDSRLFLYKRKHIPLKDGRKPWVVSGHANDIEYMDYDDMHNV
                     HLKDLLPMDLEFDMNFHILSFDPAASHPFVETHYQGHGAY"
BASE COUNT         1385 a          872 c         1064 g         1165 t
ORIGIN      
        1 ctcgcgtgca tataaagggc ataccgcttt tcaatggggc cgaccgagat gatctcattt
       61 gaaacaagca tttttgcaag ttctcttggc acgttcgtca ttgctgcata gtgggggctt
      121 ggctttaccg cccaatcccc ggttatatcg ccgtatccgt aaaatgaaac gatcgctttc
      181 ggcttgttcg ggaaagtccc agtcagaagg gcgaggaaac cgcctgctga actgccgatg
      241 accgctattt tttccggatc ataatcaaac tgctttgtcc cttcgtttcc gatccaatca
      301 agggcatcga cgatatcctc tcgaatggca ggcagcttgg attcaggcgc taaccgataa
      361 tcgatcgaaa agatattgaa accggcttga tgataaaaat caatttgcgc gggcttcata
      421 tcttcacggg aaccccataa tagtcccccg ccgtgaatat agacaatgac cggacgggat
      481 gcttccgaag cggggtaaaa gtccgctttc agctcgaagt tatcctgttg tttataaatg
      541 accgttgttt ttgacatgat gtcacgctct ttctgtcgtt tagatatgct ttctgtaagt
      601 attgtataat aaggataata atatttcgag ataagaactt taggatagtt ggaaaggggt
      661 gcgacgaatg gagttcaaag taatggattt attggcgttg gagtcgatga aggatgctga
      721 agtgttaagc gggggaaatt atatccataa tgtgatcgaa ggcgtgacga tcatggaagg
      781 gcccgatatc gcgaattgga tcaaaggcgg ggaagtgatt ttaacaagtc tttattcgat
      841 ccggaacttt agtgaaaatg aacaaaagga tttcgtttcc aagctggctg aaaaaagagt
      901 cagtgcgctt gtcattaagg aacatagtga agaaatttct actcaattgc ttgaagcggg
      961 cgagagaaac aggatgccga tcattcagct tccgcaagag gttccatttg tcgatgtcat
     1021 gtatccgatc atgggggagc tgtttaataa acaagttaca aaactccaat attataaaga
     1081 gattcacgat cgatttacag ctttgtcttt agctgataaa ggccttgaga aaatcattca
     1141 tacacttgaa gagctgatcg ggaatccggt cgccttgttc gaccgtaatt tcagttgtat
     1201 cgtttcgaca tatcctgagc ttgcgaactt tgaaatggtg gagaaagtcc atttttacga
     1261 gcaaacatca ggaatcaaat ttccccacta tcggcaaatt gtaaaatatc cggatctgga
     1321 taatattaaa gggcatcaaa tcgtcgttcc gatagaagcg ctgaatcata ataaaatgta
     1381 tttactaatc ggtgagattc ataaatcact tggcgaactg gacttgattg ccgtggaaaa
     1441 cgctgcgact tccttgtccc tcgaattagt gaaacaattt gcggttgcgg aagttgaaaa
     1501 gaaatataaa aacgatttga tcgaggaatt gataagcgga agaatccaat cgatccaagc
     1561 tgtatatgaa aaggcgaatg tgattggctg ggacttcaat ggatcatttg ctgcggttct
     1621 attcaaaatc aatcgccaaa cagagagcat gttaaaacaa aaaggcgatc ttgccgaccg
     1681 gagccacttt ctcgtgagtg aagcgattca tcattatttg ccgaacggca tcatcacgaa
     1741 taaaagcaat cttttcattg tgctatggaa aatcgagaag acgagtaaaa atgacaatgc
     1801 atggatgaat caaattaaga gaaccgccct gacgattcag acggtcgtta ataaacaagt
     1861 gaatgacatc gacgttcaag tggggattgg cagtgcggta agtgatatta ccgaaatccc
     1921 gaaaagctat cgggaagccc atgatgcgct ggatctaggt gaaacattga atggtctgtc
     1981 atcaatcacc gccttttcgg aattgggtat tttccggctg ttgcgccatg tgaaagattc
     2041 ctccgcattg ctgcaattca ttccaaaatc attgaaggaa ttgctcgatt accaacaggc
     2101 gaatcaatcg gatttgctgg aaacgctgca aacctttttg gaatgcaatc aaaacgcaac
     2161 acaaacagcc aaacaccttt ttgtccatta taagacggtc atgtatcgct tggagcgaat
     2221 caaggaaata acgggaatgg actttgatga ttcagaggaa atgctttcag tgcgggttgg
     2281 actgaagatt tatgaactga ttgaacgcga aaaccgacaa tattaatgga ggcagccgta
     2341 tgcgctgtct cttttcattt gcacttatcc actttgggta aatttcagat gaaactttat
     2401 ctacacatgc taaagactat tcagtttata accattacaa ttagtagtgg agctaaaggc
     2461 caaaagtaaa tcatagcaat tcattgcata catccgtatg gattacagac aataattagc
     2521 gttacaaact attataagca tttacaaggc gcggaaaagg gtggtgaaat aggtgaacga
     2581 tatgaaggac attgtacaaa agcttgatct tgcagacgaa acagccggca gactggattg
     2641 gctcggagaa tttggcaaag atcctgaagg cggtgtttct cgtttactct atacaaaaga
     2701 gtgggttgaa ggtcaacacg cattaaaaga atggatggaa agcgaaggcc tggatgctcg
     2761 atttgatgaa attggcaact tgtatggaac cttgaaaggg aaaaatccaa ctgaaacagt
     2821 actgacaggc tcccatgtgg ataccgtgac aaacggcggc cgttatgacg gtgcatatgg
     2881 aattgtagcc ggaatcatcg cattgaaata cttgaaaaag catttcggcg aaccagtcag
     2941 aaacatcgaa gtcgtttcga tggctgaaga ggaaggaagc cgcttcccat actcgttctg
     3001 gggatccaaa aacattgtcg gcttggctaa acgtgaagac gttgaatcga tcaaagattt
     3061 tgacggcgtt ccgtttgtcg aagcgatgaa aaatgccggt ttcgacttta aagacaatgc
     3121 cgagggacgt aaggatttaa aagcgttcgt tgaagttcac gtagaacaag gaagcgtttt
     3181 agagacggaa aatacggcaa ttggtgtagt gaacaacatt gttggacaaa gacggtatac
     3241 ggtcgaaata acaggtcaag ctaaccatgc cggtacaacg ccgatgggct accgaaaaga
     3301 cgcactttat gcatctagcc atatgatcca tgaaattatt tcgatggcta gaaaagtcgg
     3361 tgatccactt gtaacgacag ttgggaaaat cgaagcttat ccaaacatcg tcaacgtcgt
     3421 tcctggaaaa gctattttca caatcgatgt ccgtcatact gataaagaag cgatcgtttc
     3481 attcacagag caaatgacgg ctagaatgca ggacatcgca aaagagcatg aagtagaaat
     3541 gaacatcgac ctatggatgg atgaagatcc ggttccgatg gatcaaacgg tcgttgaact
     3601 tattgaaaag caatgtaaag taaatggcct caactataag ttgatgcaca gcggagctgg
     3661 acatgattcg caaatcttcg cacctgtcgt accgacagcg atgctgtttg ttccgagtct
     3721 aaaaggcatc agccataatc cggctgaatt tacggagcca tcggatttgg cggaaggtgt
     3781 aaaagcgctt atcggcgccc tttacgaatt agggtataaa gaataacttt tttgaatccc
     3841 gggtgcaact cccggaaata taaacaaata gaggagaatg taacgatggg ttatccaaaa
     3901 gatatacttt caagcaggtc aattattaaa ccaggtcaat atgcgctaat cgctccagaa
     3961 ggcttagtga ataacgttat ccctggattt gaaaattgca gaatctccat tttagcttca
     4021 ccgaaactgg gtgccggctt tgtcgactac atggtcacaa tgcatgaagg cggaaagaac
     4081 agcgaaggtt tctgcggtga agaagacgta cagtgctttg tttatgtgat tagcggaaaa
     4141 gtgaaagcga aagctgatga tcaagaattc attcttgaag agagcggcta cctatattgc
     4201 ccaccaggcg taacaatgta tttggaaaac atggagtccg gtgattcccg tctattcctt
     4261 tacaaacgta aacatattcc attgaaagac ggacgcaaac catgggtcgt atccggccat
     4321 gcgaatgaca tcgagtacat ggactatgac gatatgcaca acgttcacct gaaagatcta
     4381 ttgccgatgg atcttgaatt cgatatgaac ttccacattc tttcattcga tcctgcagca
     4441 agccacccgt ttgttgaaac gcattatcaa ggacatggtg catatc
//
LOCUS       DASULI010000043         5620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_104795, whole
            genome shotgun sequence.
ACCESSION   DASULI010000043 DASULI010000000
VERSION     DASULI010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104795"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      91..136
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            143..628
                     /locus_tag="VFX34_00930"
     CDS             143..628
                     /locus_tag="VFX34_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003196485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEX5563494.1"
                     /translation="MTVKTMATSGIIAALYVAVTFLIAPFGFTNIQFRLSEMFNHLVV
                     FNKKYIVGIVLGVFVANLLFSELGPIDLVFGVGQSILALSITILSAKFIKGLYRRMFF
                     NTIVFTFTMFLIAWELNIVLEFPFLLTWLFVAVGEFVVMAVGIPVMAALNKRLQFEKL
                     I"
     gene            679..909
                     /locus_tag="VFX34_00935"
     CDS             679..909
                     /locus_tag="VFX34_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEX5563495.1"
                     /translation="MYMTIEETAEYLGMPIEQVRKYVWEGRIRSVHDGEQFLINQDQF
                     SNYFNQLERLKKQIEDYLNEPLPPDRDIKDED"
     gene            1176..2693
                     /locus_tag="VFX34_00940"
     CDS             1176..2693
                     /locus_tag="VFX34_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEX5563496.1"
                     /translation="MRKISNVFYITIGLIILAVGYGVAAADSFEAVTTSIKSFVSSSF
                     GWYYMLLLSFLLVLSIFFIFSPFGKVRLGEDTDRPTFSTITWIAMLFSAGMGIGLVFY
                     GAAEPLSHYAINPATAEPNTDAAFKEAMRQTFFHWGLHVWAMYGIVGLALAYFQFRKK
                     EPGLISATLKPIFGKKMEGPWGVLVDVLAVFATAFGVATSLGFGAVQINAGLNYLFGF
                     SIGFTSQLIIIAVVTVLFVMSAWSGLSKGIKYLSNTNLILALALLGFVLILGPTLLIF
                     NVFTDSIGGYLANLVSMSFRTAPLNNAHREWLESWTIFYWAWWISWAPFVSMFIARVS
                     KGRTIREFMVVVMLAPTILSAIWFSAFGSTAINMQRKGIADLANTDTELTIFEMFHAM
                     PLSIIISIVAVLLIASFFITSADSATFVLGMQSTYGSLTPPNSIKIIWGVIQSTIAII
                     LLSVNGLTALQNTIIIAALPFSFIILLMIVSLLKALKSELPKSERPRRRKAKDSI"
     gene            complement(2742..3728)
                     /locus_tag="VFX34_00945"
     CDS             complement(2742..3728)
                     /locus_tag="VFX34_00945"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010287274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5563497.1"
                     /translation="MPSIKVAILGFGTVGEGVYRILQEKREEIQKSTGFTVDVVSILV
                     RDKAKERMPIIGAKFTDDIQDILNNPEIDIVLEAIVGEEPAYTYLSDAIEKGCHIITA
                     NKKMFAIHGPALLKHAEFRNVQVGFEATTAGGVPIIRTVTNLLAGDRVKRIQGILNGT
                     SNFILTKMRDEGSSFKQALSDAQQKGFAEADPTDDVSGTDAFRKLMILSSLAFGKQPD
                     WEDVPVVGIDHLESIDINDATKGDLRYRHVADIWKDEKGKLQATVGPVLIGADHPLFS
                     IDGVDNAVIVNTEYLGPLTLVGPGAGMYPTGSAMVADLLHIIRQHEAVLVPN"
     gene            complement(3774..5072)
                     /locus_tag="VFX34_00950"
     CDS             complement(3774..5072)
                     /locus_tag="VFX34_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEX5563498.1"
                     /translation="MTNQQLQPETLLLHGGQKPDPVTGSRAVPIHRTTAFVFRDTEHA
                     QNLFALQEAGNIYTRITNPTVAVFEERVALLEGGTAAVALSSGMAAIAFSILNIAGAG
                     DEIVAAGNLYGGTYNLFAVTLPRYGINVKFVDAEDPENFRAAITDKTKAIFAETIGNP
                     SLQVLDIEAVANIAHENEIPFLIDNTFASPYGSNPIEFGADVVIHSATKWIGGHGTTI
                     GGVVVDAGKFDWTRGKFPGFTEPDATYHGLRYGIDTAGAAFATKLRVQLLRDFGPCLD
                     PDSAFNFLQGLETLHLRVTRHNENAVKVAEFLQAHPSVEWVTYTGLEDHPTYENAKKY
                     LKNGFGSIIVFGIKGGREAGRKVIDNITLWSHVANVGDAKSLIIHPASTTHQQLSAED
                     LKKTGTPEELIRLSVGLESTEDIIADLKQAIEKATPVEAL"
     regulatory      complement(5192..5307)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1400 a         1305 c         1281 g         1634 t
ORIGIN      
        1 aattgtcgga aacctataga atgcgaggga gaaatgtata ttgctattga acattcaata
       61 gtcctatgat aaattaaaag ataggctttt tgccacgtgg ttcgaaaacc tcccacgtaa
      121 aaaaaactaa ggagaaatga atatgacagt aaaaacgatg gcaacgagcg gaatcatcgc
      181 tgcgttgtat gttgctgtga cgtttttgat tgccccgttc gggttcacta acatccagtt
      241 ccgcttatcg gagatgttca accatctcgt tgtgtttaat aaaaaatata tcgtcggcat
      301 tgttttaggt gttttcgtcg ccaatctgct tttttctgaa ttggggccga tcgatctcgt
      361 atttggcgtc gggcaatcca tactcgctct atccattacg attctatcag cgaaattcat
      421 caaaggactt taccgacgga tgtttttcaa tacaatcgta ttcacgttca cgatgttcct
      481 catcgcatgg gaattgaaca tcgtgctgga gtttcctttc ttgcttacat ggctcttcgt
      541 cgcagtcggc gaatttgttg tcatggcagt cggcattcca gtcatggctg cgttgaataa
      601 acgacttcag ttcgagaaac tgatttaaaa agaggcgaat tgtcgcctct tttttttcga
      661 tgaaaggaga gtattaggat gtatatgaca attgaagaaa cagcagaata tttgggaatg
      721 ccgattgagc aagtccggaa atacgtatgg gaaggacgca tccgttccgt ccatgatggt
      781 gaacaatttt taattaatca agatcaattc agcaattatt tcaaccagct ggagagatta
      841 aaaaaacaga tcgaagacta tttgaatgaa ccgcttcctc ccgatcgaga tattaaggat
      901 gaggattaat aacttgtata gagggtaaac tgaaaacagg gatatgccgt agtcaaaaag
      961 acacaatttt ttcatgcaat cgaacgcagt tttccatttc gcaattttta ttatatttct
     1021 aactggctct tttataatgc aggttgttcg acttaatgaa aaaataaaac gagctataca
     1081 tgtaagtgtt tacattttga ccccttttgc attcccgcta catgattggt atagtatgta
     1141 gtcgtttcaa aaaaccatta ttcatagggg gaaacatgcg aaaaatatca aacgtgtttt
     1201 atataacaat cggcctgatc atattggctg taggctatgg agttgctgcg gcggatagct
     1261 tcgaagcagt gacaacttcc atcaagtcct tcgtttcatc ttcatttgga tggtattaca
     1321 tgcttctgct ttcatttctt ctggtgctaa gcattttctt catcttcagt ccattcggca
     1381 aagtgcgttt aggagaagac acggatcgtc caacgttttc gacgatcaca tggattgcca
     1441 tgcttttttc tgccggaatg ggaatcggat tagtcttcta cggggcagca gaaccgctat
     1501 cccattatgc catcaatcct gcaacggctg agcccaatac tgacgccgct ttcaaagagg
     1561 cgatgcgtca gacattcttc cattggggat tgcatgtatg ggcgatgtac ggaattgtcg
     1621 gacttgcact ggcatatttc caattccgta aaaaggaacc ggggttaatt tcagcaacat
     1681 tgaagccgat tttcggtaaa aaaatggagg ggccatgggg cgtcctcgta gatgtacttg
     1741 ccgtatttgc aacagctttt ggcgttgcca cttctctcgg cttcggtgct gttcaaatta
     1801 acgcagggtt gaattatctg tttggcttct caatcgggtt cacttcacaa ctaatcatca
     1861 tcgcggtcgt cacagtccta tttgtcatgt ccgcttggtc ggggcttagc aagggtatca
     1921 aatatttgtc gaatacaaac ctgatacttg ccctcgcctt attagggttt gtccttatcc
     1981 tagggccgac tttgcttatc ttcaatgtgt tcaccgattc aatcggaggc tatcttgcca
     2041 atctcgtttc gatgagcttc cgtactgctc cattgaacaa tgcgcatcgt gaatggcttg
     2101 agagctggac gattttctat tgggcttggt ggatctcctg ggcgccattc gtcagtatgt
     2161 ttatcgcacg ggtatcgaag ggtagaacga tccgtgaatt tatggtcgtc gtcatgcttg
     2221 cgccaaccat cctgagcgcc atctggtttt cagctttcgg atcgaccgcc atcaatatgc
     2281 aacgtaaagg gattgcggac ctagcgaata cagacactga attgaccatt tttgaaatgt
     2341 tccatgcgat gcctttgtca atcatcattt caattgtcgc agttctgctc atagcgtcat
     2401 ttttcatcac ttccgcagac tcagcgacat ttgtgttagg catgcaatcc acatacggtt
     2461 cattgacacc gccaaatagc ataaaaatca tttggggtgt catccagtca accattgcga
     2521 ttatcttgtt gtccgtgaat ggattgaccg ctctgcaaaa cacaattatc attgctgccc
     2581 tgccgttctc tttcatcatt ttgctaatga tcgtttcatt gctcaaagca ttaaagagcg
     2641 aactgccaaa gagtgaacgg ccgaggagaa ggaaagcgaa agatagcatc taacgacata
     2701 gaaaaagtgg aggggaatcc aacaattcct cctccactta tttaatttgg caccaaaacc
     2761 gcttcgtgtt ggcgaatgat atgcaacaag tccgcaacca tcgcgcttcc tgtcggatac
     2821 attcctgcac ccggtccaac taacgtcaat ggtccgagat attcggtatt tacgatgacg
     2881 gcattgtcaa caccgtcaat actgaaaaga gggtgatcag ctccaattaa gacaggtcct
     2941 accgtcgcct gcagcttccc tttttcatcc ttccaaatat ctgccacgtg acgataacgg
     3001 agatcgcctt tggttgcatc attgatatca attgattcga gatgatcgat tccgacgaca
     3061 ggaacgtcct cccaatccgg ctgcttgccg aaagcgagtg agctcaaaat catcagctta
     3121 cggaaagcgt ccgtgccact cacatcatct gtaggatcag cctctgcgaa acctttttgc
     3181 tgcgcatcag aaagcgcctg cttgaaagaa gatccttcat cgcgcatttt cgtcaaaatg
     3241 aaattcgacg tcccatttaa aatgccttgg atccgtttta cgcggtcgcc tgcaagaaga
     3301 ttggtcactg tccggataat cgggacaccg ccagctgtcg tcgcctcgaa tccgacttgc
     3361 acattgcgga actcagcatg tttcagtaac gcaggaccat gtatcgcaaa catctttttg
     3421 ttcgctgtga taatatggca gcctttctcg atcgcatcag acaaatacgt atatgcaggc
     3481 tcttccccga caatcgcttc aagaacaatg tcaatttctg ggttattcaa aatatcctgt
     3541 atgtcatccg taaacttcgc gcctataatc ggcatgcgtt ctttcgcctt gtctctcaca
     3601 aggatggata cgacatcaac ggtgaaacca gtgctctttt gaatctcttc ccgcttctcc
     3661 tgcaggattc tgtagacacc ttcaccgacc gtaccgaaac cgagtatggc cactttgatt
     3721 gacggcatta ttccaccctt cctttctatc tatccttttt cattcctaac acgtcatagc
     3781 gcttcaactg gcgttgcttt ttcgatcgcc tgcttcaagt ctgcaataat atcttcagtg
     3841 gactcaagac cgacagacag acggatcaat tcctccggcg tacccgtttt cttcaagtct
     3901 tccgcagaca attgctgatg tgttgtcgat gcagggtgga tgatcagcga ttttgcatcc
     3961 cctacgttcg ccacatgtga ccatagcgta atattgtcaa tcactttgcg ccctgcttca
     4021 cggccgcctt taattccgaa tacgatgatg gaaccgaagc cgtttttcaa atatttcttg
     4081 gcgttttcat acgtcggatg atcttccaat cctgtgtaag taacccattc aacagacgga
     4141 tgagcctgta ggaattcagc gactttcacg gcattttcat tatggcgtgt cacacgcaaa
     4201 tggagcgtct ccagcccttg aaggaagttg aaagcactgt ccggatctaa gcatggaccg
     4261 aagtcgcgca gcaattggac gcggagcttc gttgcgaatg ccgctcccgc cgtgtcaatg
     4321 ccgtagcgca atccgtggta cgttgcatcc ggctccgtga agcctgggaa cttgcctctc
     4381 gtccagtcga atttcccagc atctacgacg acgccaccga ttgtcgttcc gtggccgccg
     4441 atccatttcg tagcggaatg aatgacgaca tctgctccga actcgattgg attcgatccg
     4501 tatggggacg cgaatgtgtt atcgatgagg aacgggattt cattctcatg ggcgatgttt
     4561 gcgaccgctt cgatatcgag cacttgaagg ctcgggttcc cgatcgtttc agcgaaaatc
     4621 gctttcgttt tgtctgtgat tgccgcgcgg aagttttcag gatcctctgc atcaacgaat
     4681 ttcacattga taccgtagcg aggaagcgtg acggcgaata gattatacgt accgccgtat
     4741 agattgccgg cagcgacgat ttcgtcccca gctcctgcaa tgttcaagat ggaaaatgcg
     4801 atggcagcca ttccggaaga tagagcgact gctgcagtcc cgccttctaa aagcgcaacc
     4861 ctttcttcaa atacagcaac ggtcggattc gtgattcttg tgtaaatatt gcctgcttcc
     4921 tgcaaggcaa ataagttctg ggcatgttca gtatcgcgga aaacaaatgc ggtagtacgg
     4981 tggatcggca cagcacgcga acctgtaaca ggatcaggct tttggcctcc gtgtaaaaga
     5041 agagtttctg gttgaagttg ttggtttgtc attttcatta cctccagtgg attttttaag
     5101 atgaaaaggc aatgcggagg cgttgggggt ttttggaata aaaaaataac ccctttcgct
     5161 aagagaaggg ttacgctttc gcttattcct cctcttatct ttcagaccgt ttgcacgaat
     5221 ctgtaggaag tagcaccttt gcaagttcgt cacttgccgg ttgccgggca tcgcagggcc
     5281 tagtccctcc gccgctcttg ataagagttt gggtattcag tttttcatct tgttgaacag
     5341 tataggagac tattttttaa aagtcaaggg gtgaatttat ttttttctga aacttgcgct
     5401 ttatctgagg tggtataatg gtgttgatag tcagaccaac ggattgagcg gtgggatgcc
     5461 tgttttgagc gcgagagtag cttctttgag cgcagatgta gcctctttga gcgcgagagt
     5521 agcttctttg agcacggatg aagcctcttt gagcgccaga gcagcttttt tgagcgcgta
     5581 gccacctgct ttggacgcga ttcagccaac attggacgtc
//
LOCUS       DASULI010000044         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1680863,
            whole genome shotgun sequence.
ACCESSION   DASULI010000044 DASULI010000000
VERSION     DASULI010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680863"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFX34_00955"
     CDS             complement(<1..458)
                     /locus_tag="VFX34_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5563499.1"
                     /translation="MKTGVLFLHGFTGGMFEVRPFIQYVEERTDWITSVPILPGHDFP
                     VDLSKVSAENWMMEAELALNRLRKEADRIIVVGFSMGGIIALYLALRYNIEKLVLLSA
                     AAKYISPRYLLEDAAVMLSESVKKNYPPNTFYHLYDYKLRHTPLHAAKEFV"
     gene            complement(455..1828)
                     /gene="murF"
                     /locus_tag="VFX34_00960"
     CDS             complement(455..1828)
                     /gene="murF"
                     /locus_tag="VFX34_00960"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5563500.1"
                     /translation="MKRLLTELAEWLQSEGQRMDEVIVSGVSFDTRTLKEGDLFIPFR
                     GELVNGHQFVRQAIEKGAAASLWMKDEPDAPADIPLIFIDDPELALQQLARSYREKLA
                     CKFIGITGSNGKTSTKDLVASVLSPYFNVRKTEGNFNNELGLPLTILSLEENTEVAVL
                     EMGMSGFGEISFLSDLARPHYAVITNIGEAHMQDLGSREGIAIAKFEIIDGLQEGGKL
                     FYDGDEVLLQALVSAHPELDAVSFGHSEDAGLSLGAMESGDEGSRFTVKGRIDGSFTI
                     PVFGSHQVKNSLAAILIAHELGLSNEQIDSALRQAKLTDMRMQPVVAENGALFINDAY
                     NAAPTSMEAALSFLQETRLRPEKWAVLGDMLELGENERHYHESIARQLGTISLKGILL
                     FGPRMEWLYEELKKNGNETKVIWSESDYKPLIATLLSSIDENSIVLLKGSRGMALEKI
                     MDGVLKA"
     gene            complement(1841..2905)
                     /locus_tag="VFX34_00965"
     CDS             complement(1841..2905)
                     /locus_tag="VFX34_00965"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5563501.1"
                     /translation="MKKKLGLVYGGKSAEHEVSLSTATAVTNAIDFDKYEVIPVYITY
                     EGEWRKGEPLASPVESMEQLRLVRDGGRPDCIQDFLDGNGTPDIILPLLHGTNGEDGT
                     VQGLFEVMNIPYAGNGVLSSSAGMDKVIMKQLFAQVGLKQVPYVHFIRNQWRKDQNYW
                     IEKMEKELTFPMFVKPANLGSSVGISKATDHDSLITAVEFALKFDRKIIVEQGVTARE
                     VEVGILGNDDPACSVLGEIKPVAAFYDYEAKYQDGNTELLIPAPVTDKVETTLKDMAI
                     RAFKVLDCAGLVRADFFVTDQDEVLINEINTMPGFTPTSMYPLLWQNTGLSYPELIDR
                     LIELALERHEEKKQIQYTMD"
BASE COUNT          698 a          823 c          654 g          784 t
ORIGIN      
        1 acgaattcct tcgcggcatg gagtggcgta tgccgtaatt tatagtcgta caaatgatag
       61 aacgtgttcg gcggatagtt ctttttcaca gattccgaca gcatgactgc tgcgtcctca
      121 agcaggtagc gcggactaat atactttgcc gctgcactga gcaggacaag cttttcgatg
      181 ttgtagcgca aagctaaata aagcgcaatg atacccccca tcgaaaagcc cacgacgatg
      241 atgcggtcgg cttccttcct caaccggttg agggcgagct ccgcctccat catccaattt
      301 tcagcagaaa ctttgcttaa atcgaccgga aaatcatgtc caggcaaaat tggcacggag
      361 gtgatccagt ccgtcctttc ctctacgtat tgaatgaaag gacggacctc gaacatccct
      421 ccggtaaatc catggaggaa taggacgcct gttttcatgc tttcaaaaca ccatccatga
      481 tcttttcaag cgccattccc ctcgatcctt tcaacaagac tatcgaattc tcgtcaatgg
      541 aagagagcag cgtcgcaatc aacggcttat agtcgctttc cgaccagatg acttttgttt
      601 cattcccatt tttcttcagc tcttcataaa gccattccat tcgcgggcca aataaaagga
      661 ttccctttaa gcttatagtg ccaagctgcc gggcgatcga ttcatggtaa tgacgttcat
      721 tttcaccaag ttccagcata tctcccaaaa cagcccattt ttcaggacgg agcctcgttt
      781 cctgcaagaa ggaaagtgcc gcctccatcg aagtaggtgc cgcattgtat gcgtcattga
      841 tgaaaagcgc accgttctct gctacgaccg gctgcatccg catatccgtg agcttcgcct
      901 gacggagtgc cgaatcgatc tgctcgttgc taagaccgag ttcatgcgca atcagaatcg
      961 ccgcaagtga atttttcact tggtgtgaac cgaaaacggg aatcgtaaaa ctgccatcga
     1021 tccttccttt gacagtaaat cggctgcctt catcgcccga ttccatcgcg ccgagagaaa
     1081 gacctgcatc ttcgctgtgc ccaaatgaaa ccgcgtcaag ctcaggatgg gcggatacga
     1141 gcgcttgcaa tagaacctcg tcgccatcat agaaaagctt cccaccttcc tgcagtccat
     1201 cgatgatttc aaatttcgcg attgcaatcc cttcacggga accgagatct tgcatatgcg
     1261 cttcgccgat attcgtaatg acggcgtaat gcggtctcgc aagatcagat aagaacgaga
     1321 tttcaccaaa accgctcatc cccatttcaa ggacggcgac ttccgtattt tcttccaatg
     1381 aaaggattgt caatggcaac cctaattcat tattgaaatt gccttccgtc tttcgcacat
     1441 tgaaataagg ggaaaggacg cttgcaacca aatccttcgt tgacgtcttg ccgttcgatc
     1501 cggtgatgcc gatgaatttg catgcaagct tttcgcgata ggaacgcgct agctgctgca
     1561 gcgcaagttc cgggtcatcg atgaaaataa gggggatatc cgcaggtgcg tccggttcat
     1621 ccttcatcca gagtgacgct gccgcccctt tttcaatcgc ttgccggacg aattggtggc
     1681 cgttcaccag ttcgccgcgg aacgggatga acagatcgcc ttctttcaac gtgcgcgtat
     1741 caaaggacac cccgctcaca atcacttcgt ccatccgttg cccttccgac tgcagccatt
     1801 ctgcaagttc agtcaataaa cgtttcacaa tccatcacac tcaatccatt gtatattgta
     1861 tctgtttctt ttcttcatgc cgctcgagtg caagttcaat cagcctgtcg atcaattccg
     1921 gataagatag gcccgtgttc tgccatagca gcgggtacat gctcgtcggc gtgaagcccg
     1981 gcatcgtatt gatttcattg atgagcactt catcctgatc cgtcacgaag aagtctgccc
     2041 gaaccagccc tgcgcaatcg agcactttga atgcgcggat cgccatgtct ttcaatgtag
     2101 tctccacttt gtccgtcact ggtgcaggga tgagcaattc cgtattgccg tcctggtatt
     2161 tcgcttcgta atcataaaat gccgctaccg gtttgatttc cccgaggacg gagcacgctg
     2221 ggtcgtcatt gccgaggatg ccgacttcga cttcacgtgc agtgacccct tgctcgacaa
     2281 tgattttccg gtcgaatttc aatgcgaatt cgacagccgt gatcaggcta tcatgatctg
     2341 tcgctttact gatgccgaca cttgaaccta agtttgccgg cttcacaaac atcgggaacg
     2401 tcaattcctt ctccattttt tcaatccaat agttttggtc ctttctccat tggttgcgga
     2461 tgaaatggac gtacgggact tgcttcagcc cgacttgggc gaaaagctgc ttcatgatca
     2521 ctttatccat cccagcggaa gaagacagca cgccatttcc cgcatacgga atgttcatca
     2581 cctcgaacag gccttggact gttccgtctt ccccgttcgt gccgtgcaat agcggcagga
     2641 taatgtcggg cgtcccgttt ccatccagaa aatcctggat gcagtcaggt ctcccaccat
     2701 cgcgcacgag tcgcagttgt tccattgatt ccaccggaga ggctagcggc tctcctttcc
     2761 tccattcgcc ttcatacgtt atatagacag gaatcacttc atatttgtca aagtcaatcg
     2821 catttgtcac cgccgtggca gttgaaaggg atacttcgtg ctccgcggac tttccgccat
     2881 acactaaacc caatttcttc ttcatcaatc tccctcgctt accgttagat ttcgttcctt
     2941 tagtttacca cgaaacgac
//
LOCUS       DASULI010000045         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1124303,
            whole genome shotgun sequence.
ACCESSION   DASULI010000045 DASULI010000000
VERSION     DASULI010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124303"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFX34_00970"
     CDS             <1..241
                     /locus_tag="VFX34_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5563502.1"
                     /translation="KWPGVRVAIAHRIGELQISDIAVVIAVSSPHRKAAYEANEYAID
                     RIKEVVPIWKKEIWEDGEEWIGAQKKYPEKGVEQT"
     gene            238..471
                     /gene="moaD"
                     /locus_tag="VFX34_00975"
     CDS             238..471
                     /gene="moaD"
                     /locus_tag="VFX34_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX5563503.1"
                     /translation="MIKVNYFARLRELTGKGEETIDRDSMTVAELLAWAETAYPGFGA
                     DNVQVAVNEEYALKDDVIESGDVCAFIPPVSGG"
     gene            471..1043
                     /locus_tag="VFX34_00980"
     CDS             471..1043
                     /locus_tag="VFX34_00980"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX5563504.1"
                     /translation="MKTAGIVLAGGLSRRFGSPKAFAAWGDRYFYELSLDALTPYCEE
                     SIIVTRPELVERFSEGLNVTTDIEPFMGQGPIAGILSGMEKLRAERYIVLPCDMPFMT
                     ADVVGRLLDSHSSGVTAVVLDGKLHPLVSVWDAAVLPDLRESLENGKRRVMDVQEKHG
                     VRWIEGELLTEDDAHRIFMNANTPDILERS"
     gene            1040..2059
                     /gene="moaA"
                     /locus_tag="VFX34_00985"
     CDS             1040..2059
                     /gene="moaA"
                     /locus_tag="VFX34_00985"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5563505.1"
                     /translation="MTKVNPIVDKLGRPIRDLRISVTDRCNFRCTYCMPKEIFGDDYV
                     FLPKNELLSFEEMERFAKLFASLGVKKLRLTGGEPLMRRDLPDLIAKLTKIEGIEDLG
                     LTTNGVLLGQYAQPLYDAGLRRLNMSLDAMDPELFGKMNGRGIKPELILKNIDRARDI
                     GFTIKMNMVVQKGVNESEILPMAAYFKERGITLRFIEFMDVGNDNGWSFEKVVTKKEI
                     YEMLRAEYEMEPAEEDYYGEVAKRYRYLDNGAEVGFITSVSESFCSTCTRARLSSDGK
                     LYTCLFASSGFDLRELIRSGMSDQELLEAITGVWERRADRYSDERTEQTVKNRKKIDM
                     SYIGG"
BASE COUNT          562 a          421 c          686 g          547 t
ORIGIN      
        1 gaaatggccg ggcgtgcgtg ttgcaatcgc acatcggatt ggtgaactgc aaatttcgga
       61 catcgccgtc gtcatcgctg tatcatctcc acaccggaaa gcggcttacg aggcgaatga
      121 atatgcgatc gacaggatta aagaagtcgt gcctatctgg aagaaggaaa tttgggaaga
      181 cggcgaagaa tggatcggtg cacagaagaa atatcctgaa aagggggttg aacagacatg
      241 atcaaagtga attatttcgc gcggctgcgc gaactgactg gaaaagggga agaaacgatt
      301 gaccgcgatt cgatgacggt agcggaacta ttagcttggg cggaaacagc ttatccgggt
      361 ttcggcgcgg acaatgtcca agtggcggtg aatgaagaat atgctttgaa agacgatgtc
      421 atcgagtcag gtgatgtttg tgctttcatt cctccagtga gcggtggctg atgaagacgg
      481 caggaattgt tcttgctgga ggattgtcaa gaaggttcgg ttccccgaag gcgtttgccg
      541 cgtgggggga tcggtatttc tatgaattgt cattggatgc attgaccccg tattgcgagg
      601 aaagcattat tgtgactcgc cccgaattgg tggagcgttt ttctgaaggc ttgaatgtta
      661 caacagatat tgagccgttt atggggcagg gcccgattgc gggtattttg tcagggatgg
      721 agaagctgcg tgccgaacgg tatatcgtac tgccttgcga tatgcccttt atgacagccg
      781 atgtggtagg acgtttgcta gatagccata gttccggggt gaccgctgtc gttttggacg
      841 gaaaacttca tccactcgta tccgtgtggg atgcggctgt actcccggat ttgcgtgaat
      901 cgcttgaaaa cgggaaacgg cgggtcatgg acgttcagga gaaacatgga gtccggtgga
      961 ttgaaggcga attgttgaca gaagatgacg ctcatcggat attcatgaat gcgaatactc
     1021 cggacatctt ggaaaggagt tgacgaaggt gaatccgatt gtagataaat taggccggcc
     1081 gatccgggat ttgcggattt cggtgactga ccgctgtaat ttccggtgca cgtattgcat
     1141 gccgaaggag attttcgggg acgattatgt gtttttaccg aagaatgagc tgctgtcatt
     1201 tgaagaaatg gagcggtttg cgaagctatt tgcatctctc ggcgtgaaga agttgcgact
     1261 gacaggcggc gagccgttaa tgcgacgtga cctacccgat ttgattgcga agctgacgaa
     1321 gattgaagga atcgaagacc tcgggttgac gacaaacggt gtgctgcttg ggcaatatgc
     1381 gcagccgctg tatgatgcgg gccttcgtcg cttgaacatg agcctggacg cgatggatcc
     1441 ggagctgttc gggaaaatga acggacgcgg catcaagccg gagcttatct tgaaaaatat
     1501 cgatcgagcg agggacatcg gtttcacgat caaaatgaat atggtcgtgc aaaaaggcgt
     1561 gaatgaaagc gagattctcc cgatggcggc gtatttcaag gaacgcggca tcacattgcg
     1621 attcatcgag ttcatggacg tcggaaacga caatggctgg agcttcgaaa aagtcgtgac
     1681 gaagaaggaa atctatgaaa tgctccgcgc ggagtatgaa atggagcctg ccgaagagga
     1741 ttactacggc gaagtggcga agaggtatcg gtatttggac aatggtgcgg aagtcggttt
     1801 catcacatcg gtttcagaat cgttctgctc gacttgtacg cgggcgaggc tttcatcgga
     1861 tggaaagctg tacacttgct tgtttgcttc ttccggattt gatctgcggg agttaatccg
     1921 cagcggcatg tcggatcaag agctgctcga agcgattacc ggcgtttggg aacgccgtgc
     1981 ggaccgctat tccgacgaac ggacggaaca gacggtgaaa aatcggaaga agattgatat
     2041 gagttatatt ggtggatgaa tgatgggtgc ttcgctcgtg ggcggggcac tttttgtttg
     2101 tttggtaatg ggtatttagt cacgaaatga ctatatatct tgggaaatga ttgatataag
     2161 tcgggaaata atcaataagt ctgcccaata gattgatata agctgggaaa tgatcg
//
LOCUS       DASULI010000046         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1077401,
            whole genome shotgun sequence.
ACCESSION   DASULI010000046 DASULI010000000
VERSION     DASULI010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077401"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..761
                     /locus_tag="VFX34_00990"
     CDS             123..761
                     /locus_tag="VFX34_00990"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake domain-containing protein"
                     /protein_id="HEX5563506.1"
                     /translation="MRLSIGTTLTIDKDFTETGDKYKSKVVDFGEGFIMIDYPAHFET
                     GKTAFFMDGTQLLLSFVEDKMSYAFRAEVTGRMNKGIPMLKLTYPGDDQLIKIQRREF
                     VRVETSIDVAVNFEGVFQQFVAEDISAGGIALNIGQDDSFEEAESVQLTIALPFANDD
                     IKYVRTQAKAVRIWEKDDRRIASMQFAKIDTTDRQNIIRFCFERQLLARNEM"
     gene            1093..1770
                     /gene="cmk"
                     /locus_tag="VFX34_00995"
     CDS             1093..1770
                     /gene="cmk"
                     /locus_tag="VFX34_00995"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5563507.1"
                     /translation="MLKGISIAIDGPAAAGKSTIAKRIAQKLHYTYIDTGAMYRALTH
                     KAIQSDIDMDSDNDLADLLMDTEILLVPEKGGQAVWIDGVDCSDEIRSQQVTGAVSKV
                     ASHPTVRKLMVDKQRALANDSGVVMDGRDIGTEVLPDADLKIFMTASVDERAERRFQE
                     NEKRGIHSSLESLKEDIERRDRADSEREVSPLKKADDAILVDTTSMSIEDVVDKVIEF
                     AEMRMER"
     gene            1767..2348
                     /locus_tag="VFX34_01000"
     CDS             1767..2348
                     /locus_tag="VFX34_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5563508.1"
                     /translation="MNLYPFGKFLVSTIFYPLYRIKVIGKENFPAEGGVLLCTNHIDN
                     LDPPVVGISCPRTVHFMAKEELFKVPILKTILPKVNAFPVKRGMSDREAFRTALKLLR
                     SGNVVGMFPEGTRSKTGELGKGLAGAGFFALKGDADVMPCAIIGPYKPFRTLRVVYGK
                     PIAMAPFRESKASAEEVTEVIMNEIRALLEQHK"
     gene            2429..>2844
                     /locus_tag="VFX34_01005"
     CDS             2429..>2844
                     /locus_tag="VFX34_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5563509.1"
                     /translation="MTEEMNLDEMNGYKEGDRVTGKVTKIEDKSVLVEITGAPFDGVI
                     PISELSSLHIEKASDAVQVGDELELIITKVEDENYVLSKRKVDAENAWDSLEEKFNNK
                     ETIETEVKDVVKGGLVVDLGVRGFIPASLVEDHFVES"
BASE COUNT          835 a          538 c          754 g          717 t
ORIGIN      
        1 aagtcataaa aacagaaaag cttgaatgat ggactgtagt ccgaacgtac tgaaatacga
       61 tattggaatt tatgcctact catgagataa tggagatatc tatataaggt aggtgtgttg
      121 caatgcgtct ttcaattggc acaacgttga cgatcgataa ggattttacc gaaaccggtg
      181 ataagtataa aagtaaagtt gtcgacttcg gggaaggatt tataatgatc gactatcctg
      241 cccatttcga aacggggaaa acagctttct ttatggatgg gacgcagttg ctcctttctt
      301 ttgtggagga taaaatgtct tatgcgtttc gtgcggaagt gactggcagg atgaataaag
      361 ggattcctat gttgaaattg acataccccg gtgatgatca attgatcaaa atacaaaggc
      421 gtgaatttgt acgggttgag acatctattg atgtggcagt taattttgaa ggtgtattcc
      481 aacagttcgt tgctgaagac ataagtgctg gcggtattgc tctcaatatt ggtcaagatg
      541 attctttcga agaggcggaa agtgtacagt tgacgatcgc gctgcctttt gccaacgacg
      601 atataaagta cgtcaggact caagcgaaag cggtacggat atgggagaag gacgatcgcc
      661 ggatcgcctc catgcaattt gccaaaatcg acacgacgga tcgacagaac atcattcgtt
      721 tctgctttga aagacaattg ctggccagga atgaaatgtg aagctttaaa gaggggaaag
      781 tcccgcttgt gtgggacttt tcccttttct attaaaaagc gtaaggtggc gttcaggtgc
      841 gacaggcata aggcgaagat gcgtagtgga gctctatgcc acggagcagc ttaaacttat
      901 gacccgagca cctgccacct gaagctggat tgataaaaag cgtaaggtgg tgttcaggtg
      961 cgacaggcat aaggcgaaga tgcgtagtgg cgttctttgc cacaaagcag cttaaactta
     1021 tgacccgagc acctgccacc tgaagctgga ttgtgttaca atgttttcga attgaattcg
     1081 ggggtagatg atatgttaaa agggattagc attgctattg acggacctgc agcagcaggc
     1141 aagagtacaa tagcaaaaag gattgcacag aaattacatt acacttacat cgatacaggt
     1201 gcgatgtacc gcgctttgac gcataaagcg atccagtcgg acatagatat ggatagtgat
     1261 aacgatttgg cggatttact tatggacacc gagatactgt tggttcctga aaaaggcggc
     1321 caagccgtgt ggatcgatgg ggtcgattgt tcagacgaaa tcagatctca gcaagtgact
     1381 ggtgccgttt ccaaggtggc ttcacatccg actgtccgca agttgatggt tgataagcag
     1441 cgggcgcttg caaacgactc cggtgtagtt atggacgggc gtgatatagg tactgaagtg
     1501 ctgcctgatg cagatttgaa aatctttatg accgcgtctg tggatgaaag ggcggaaagg
     1561 cgctttcagg aaaatgaaaa gcgggggatc cattcttcgc ttgagagtct gaaagaagat
     1621 atcgaaagac gcgaccgtgc cgacagtgaa agggaagtat ccccgctaaa gaaagcggat
     1681 gatgctattc tcgttgatac aacttcgatg tccatcgaag atgtggtcga taaagtgata
     1741 gaattcgcgg aaatgaggat ggaacgatga atctatatcc tttcggcaaa tttcttgtca
     1801 gtacaatctt ctatccacta tatcggatca aagtgattgg caaagagaat ttccctgctg
     1861 aaggcggtgt tcttctctgt acgaatcata tagacaatct ggatccgcct gtcgtcggca
     1921 tttcctgtcc gcgaaccgta cacttcatgg caaaggaaga actgttcaaa gttcccatcc
     1981 tgaaaacaat acttccgaaa gtgaacgcct ttccagttaa acggggcatg agcgatcgtg
     2041 aagcattcag gaccgccttg aaactgttgc gaagcggcaa cgtagttgga atgtttccgg
     2101 aagggacaag gagcaaaacc ggtgaactag gaaaaggact ggcgggcgca ggcttctttg
     2161 cgctaaaagg tgatgcggat gtgatgccgt gcgctatcat cggtccatac aagcccttcc
     2221 gcacgttgcg tgtagtctac ggaaagccga ttgccatggc gcctttcagg gaaagcaagg
     2281 cgtctgcaga agaagtgacc gaagtcatca tgaatgaaat ccgtgccctt ttggaacagc
     2341 ataaataaat tgataaggtg aattttttgt ttttatgcat cgattcgaat aatatagaga
     2401 taggattcat tatggaggag gactacatat gactgaagaa atgaatttgg atgaaatgaa
     2461 cggatataaa gaaggcgacc gcgtcactgg aaaagtgacg aagatcgaag acaaatccgt
     2521 attggtcgaa attactggag ctcctttcga tggtgtcatc cccatcagtg aactatcaag
     2581 tctccacatt gaaaaagcgt ccgatgcagt gcaagtcgga gatgaacttg aattgatcat
     2641 tacaaaagtt gaagatgaga attatgtcct ttccaagcgg aaagtcgatg cggaaaacgc
     2701 atgggattca cttgaggaaa aattcaataa caaagaaacg attgaaacgg aagtgaagga
     2761 tgtcgtgaaa ggcggacttg tcgtcgatct aggcgtccgc ggtttcatcc cggcatctct
     2821 tgtcgaagat catttcgttg aatc
//
LOCUS       DASULI010000047         6680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1118419,
            whole genome shotgun sequence.
ACCESSION   DASULI010000047 DASULI010000000
VERSION     DASULI010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118419"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFX34_01010"
     CDS             complement(<1..330)
                     /locus_tag="VFX34_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002010389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5563510.1"
                     /translation="MPTPIEILIIEDDKRVADIHRRFIDKMEGFTVVGSAHTGEEAKD
                     WVQALAPDLVLLDVYLPDALGTEIMAFIHENSPETDIIFITAAAEVDIVKKAYRRGVV
                     DYILKPLT"
     gene            complement(314..1927)
                     /locus_tag="VFX34_01015"
     CDS             complement(314..1927)
                     /locus_tag="VFX34_01015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5563511.1"
                     /translation="MKPKSLQSRIFSYGALFVSLVAIIIGVSFYFTMSQGIEKQIGIR
                     ALSIAKTTAARPDVRAAFEEDDPSRLLQPIAEAVRRQTGAEYVVIGNSEGIRYAHPVV
                     ERIGQKMVGDDNERALHLGESYVSKATGTLGPALRGKTPVFNDEGEIIGVVSVGYLNE
                     DISSIFFQYIDNISYIVLIAIGLGIIGSSFLSRNIKKELFDLEPTEIANLFTERNALI
                     ESVREGIIMVDAKGIITTANAAAYDTLSIPGGQTDLIGRSIQEVLPNSLLPKVLETGE
                     RHLDRPMEIHGRKIIVNRIPIRADEQVIGAVSTFRLQSEIDQLSMELSQVKRYTEALR
                     AQTHEYNNFLYTISGLIQLHSYDEALSLIHDETAEHQSLIQFVTKRLQDPFLGGIVIG
                     FFNRARELKVRFLLDEDSFLKELPGHLEKTHFVSVIGNLVTNAFEAVEMNPEAKRMVR
                     LFIFDNGDEILFEVEDSGKGIADELLDDLFREKVSTKDVEDRGYGLMKVAENVEDLGG
                     SITVEKGDLGGALFIISIPKRGYIYADSN"
     gene            2193..2426
                     /locus_tag="VFX34_01020"
     CDS             2193..2426
                     /locus_tag="VFX34_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563512.1"
                     /translation="MKEFKITYFFDEEHYIRRFIHTDSLESAEQLVNSERDQYISFRD
                     SRGIYHELHTGKVRVVQVSEYFREDKMKFANKQ"
     gene            complement(2584..3903)
                     /locus_tag="VFX34_01025"
     CDS             complement(2584..3903)
                     /locus_tag="VFX34_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5563513.1"
                     /translation="MKTAVSTPVETLFVNSFIDGVLDPAKDMLGPVKDGGQIVANTAP
                     GCWGPMITPCIKGGHEVTTPVFVEGAEVGDAIAIRIKSIQVTSMATSSGHDQPVEGRF
                     LGDPFVAVKCPGCGTMYPDTVIKGIGQEAIRCAACDGDITPFTFTNGYTITFSDRGDV
                     GLTVTKEAAEKIGQNGRHYMGMPEASVQNPIVTFAPHDLVGTVARMRPFLGQLGTTPS
                     MPMPDSHNAGDFGSFLVGAPHEYAVTQEQLDEHRTDGHMDISRVRAGATLICPVKVPG
                     GGVYIGDMHAMQGDGEIAGHTADVAGIVHLQVNVLKGMNIEGPILLPNEEDLPYTAKP
                     LSASEKQSARALAAQWGVEEIEESLPISVVGTGTDLNVATANGIARAAALFEVPVEEI
                     MNRATITGSIEIGRHPGVVTVTFQAPRTYLEKANLYEVVDEQYRNRD"
     gene            complement(4006..5415)
                     /locus_tag="VFX34_01030"
     CDS             complement(4006..5415)
                     /locus_tag="VFX34_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing diguanylate
                     cyclase"
                     /protein_id="HEX5563514.1"
                     /translation="MEKEDLVSLQRNVLKLRVEGKYEETIEACFRLLEAGLAANDPKS
                     ILTAHINNAASFYCIGAIEEAFHSIDAYIEYCADKGDEADRLNGCNVLFLLHEYNKDY
                     EKAKETLERAIELGEKLKHYNIVSNAYSNYSMILAIESDFENALMMAEKGLAMAKLHE
                     PRTPILEFRVKLNVANALIGLDQLGTAGQLVDEMVNDPLLDSFIREKTQVHDLNGRYL
                     MKKEMYPEALQAYDTAKEIAESYKDTNLLKGILEKRCTLCELMGDVQRGYKAQQAYIN
                     LLSDLRQHEVTMAAVKIGVKHRISTIERRANTDFLTGLYNRSYMEMTTDKWLAEAART
                     GESLVCIAFDLDNLKSLNDTYGHIFGDKVIKEIGQECSSLIRNGDLMGRFGGDEFAII
                     LRNLSLEEAQHKAENLLEAVRSIKIEKDGIYISLTASVGLADNANGTIRTFTKLFHQA
                     DVALYQAKEDGKNRICIMS"
     gene            complement(5577..5798)
                     /locus_tag="VFX34_01035"
     CDS             complement(5577..5798)
                     /locus_tag="VFX34_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563515.1"
                     /translation="MIAIKKIAAILLAGAVVASSGAIFAGVGPAVDLLASFEKEYENN
                     LLKAESNMLDAAGTPSGKIANALQGEAGK"
     gene            complement(6033..>6680)
                     /locus_tag="VFX34_01040"
     CDS             complement(6033..>6680)
                     /locus_tag="VFX34_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5563516.1"
                     /translation="GKMAARLHVEVATVFDVIKLYERVTGGTASGRETILNRLFIALI
                     KKETAEQAIHFAHEAIEMHGGNGYIEDFVTPRLLRDAQVLTVWEGTANILGLELIRLV
                     NKFEAHRLFIDEMRNRLVVINDSEMKKALSVELNALTTDLTEFAAFDNALQTFEAKSL
                     MKRMAYLYESVVALEWAMKHGGKFEKLAEIYIEDTWSLRNVGDEMKTVAYFDQII"
BASE COUNT         1539 a         1759 c         1547 g         1835 t
ORIGIN      
        1 tgtgagcggc ttcaatatat aatcgacaac gccgcggcgg tacgcctttt tcacgatgtc
       61 gacttcggct gccgccgtta tgaagatgat gtccgtttcg gggctgtttt catggatgaa
      121 cgccatgatc tccgtgccaa gcgcatcagg caaatagacg tcgagcagca caaggtccgg
      181 tgccaatgcc tgcacccagt ccttcgcctc ctcgccggta tgtgcggatc cgacgaccgt
      241 gaagccttcc attttatcga tgaatcgacg atggatgtcg gccacccgtt tatcgtcttc
      301 tataatcaga atctcaattg gagtcggcat aaatataccc ccttttcggg atggagatga
      361 tgaaaagcgc accgcctaaa tccccttttt ccactgtgat ggagccaccc aagtcctcga
      421 cgttctccgc cactttcatc agcccgtatc ccctgtcttc cacgtctttc gtcgaaacct
      481 tttccctgaa cagatcatcc aatagctcat ccgcgatgcc tttgcccgaa tcctccactt
      541 cgaataagat ctcatccccg ttatcgaata tgaaaagtcg gaccatcctt ttcgcttcgg
      601 gattcatttc caccgcttca aaggcgttcg tcaccagatt cccgatgacg gagacgaaat
      661 gggtcttctc caaatgaccc ggcagctcct tcagaaagct gtcttcgtca agaaggaaac
      721 ggactttcaa ttccctcgcc cggttgaaga agccgatgac aatgccgccg aggaaaggat
      781 cctgcagccg cttcgtcacg aattggatga gtgattggtg ctccgccgtc tcatcgtgga
      841 tcaggctgag cgcctcatcg tatgaatgaa gctggatcag ccccgaaatc gtatagagga
      901 aattattata ttcatgcgtc tgcgcccgga gcgcttccgt ataccgcttc acttgcgata
      961 attccatcga aagctggtcg atttccgatt ggagccggaa tgtggacacg gctccgatga
     1021 cttgttcatc cgcccggata gggatgcggt tgacaatgat ttttcgtccg tgtatttcca
     1081 tcggccggtc aagatgcctc tcccccgttt caagcacttt cggcagaagg ctgttcggaa
     1141 gcacttcctg tatcgacctt ccaatcagat ccgtctggcc gccagggatg gaaagcgtgt
     1201 cataagcagc cgcattagcg gtcgtaatga tcccttttgc gtcgaccatg atgatgcctt
     1261 cgcgaacaga ttcgataagc gcattccgtt cggtgaataa attggcgatt tccgtcggct
     1321 ccaagtcgaa tagttctttc ttgatgttcc gggaaaggaa gcttgaaccg atgatgccga
     1381 gaccgatggc gatgagcacg atgtaagaaa tattatcaat gtactgaaag aatatggagg
     1441 aaatgtcttc attcaaataa ccgaccgaaa cgaccccgat aatttcacct tcgtcattga
     1501 agactggcgt tttccccctg agtgccgggc cgagtgtccc agtcgccttc gaaacatacg
     1561 actcccctaa gtggagcgcg cgttcattgt catccccgac catcttttgg ccgatccgtt
     1621 cgacgaccgg gtgggcatac cggatgcctt cgctgttccc gatgacgaca tactcggctc
     1681 cggtttgccg gcggaccgct tcggcgatcg gctgcagcag ccgcgatggg tcgtcctctt
     1741 cgaaagcggc tctgacatcg gggcgtgccg ccgtcgtttt cgcaatgctc agcgcccgga
     1801 tgccgatctg cttctcaatc ccttgcgaca ttgtgaaata gaacgatacg ccgatgatga
     1861 tggcgacgag cgagacaaac aaggcgccat atgaaaatat gcgtgattgc aaggatttcg
     1921 gtttcatgcg gatcatccct ctatctatct ggaagtgtag gtgcctgtcg gatttgagct
     1981 tctccggtaa tctatttctc tctatgttac actattggca attgtggggg aatagggtga
     2041 atgggaataa aaccgttaca tgctggttac gggggatggc tttgaacgcc gagccagcta
     2101 ctttggacgc gattagagta gataaagtaa tgctttggaa ttgattaagc tgaatgtggt
     2161 taccactatg ttatatgaaa ggggctgtta aaatgaagga attcaaaatc acttattttt
     2221 tcgatgagga acattacata cggcgcttca tccacacaga ctcgttggaa agcgcagagc
     2281 agcttgtcaa ttcggaacgg gatcaataca tttcgtttcg agatagccgg ggaatctacc
     2341 atgagctgca taccggcaaa gtgcgcgtcg tgcaagtttc ggaatatttc cgggaggata
     2401 aaatgaagtt cgctaacaaa caataggccc ttaaaattac ccttgccaaa acaaacgaaa
     2461 cttcatctat ctgcaaaatt cgacaaaaga aaaaactatg ccgaaatatt tcccgggaag
     2521 tttttggggg taatttaaaa acgagaagcc tcataggaca aaatcccatg aggcttttct
     2581 caatcaatca cgatttctat attgctcatc cactacttca tacaaattcg ccttctccaa
     2641 ataggtccgc ggcgcttgga atgtcacagt gacgacgcct ggatgtcgtc cgatttcgat
     2701 ggagccggta atcgttgcgc ggttcatgat ttcctcgact gggacttcga agagcgccgc
     2761 tgcgcgggcg attccgtttg cggttgcaac gttcaaatcg gtgcctgtgc cgacaacaga
     2821 gattggaaga ctctcctcga tctcctccac tccccattgt gcggcgagcg ctctggcaga
     2881 ttgcttttcg gaagccgaca gcggttttgc cgtataaggc aagtcctctt cattcggcaa
     2941 cagaatcggc ccttcaatgt tcatcccttt caagacgttc acttgcaaat ggacgattcc
     3001 tgcaacgtca gccgtatgcc cggcaatttc gccatcccct tgcatcgcat gcatatcgcc
     3061 gatgtaaaca ccgccgcccg gcactttcac aggacagata agtgtcgcac ccgcacgcac
     3121 ccggctaata tccatatgcc cgtccgtcct gtgctcatcc agctgttcct gtgtgactgc
     3181 gtattcatgc ggtgcgccga caaggaatga tccaaagtcg ccggcattat gcgaatccgg
     3241 catcggcatc gatggcgtcg tgccgagttg gccgaggaaa ggacgcattc ttgccactgt
     3301 gccgaccagg tcatgcggtg caaatgtgac aatcgggttt tggacggatg cttccggcat
     3361 gcccatataa tgcctgccgt tttgaccgat tttctctgct gcttccttcg tcaccgtcaa
     3421 gccgacatcg ccgcggtcgc tgaatgtgat cgtatagccg ttcgtgaatg tgaatggcgt
     3481 aatgtccccg tcacatgcag cgcagcggat cgcttcctgc ccgatccctt taatgacagt
     3541 atccggatac atcgttccgc atcccgggca tttcaccgcc acaaatggat cgccaaggaa
     3601 gcggccttcg acaggctggt catgtcctga agatgttgcc atcgatgtca cttggatcga
     3661 tttgatccgg atggcaatcg cgtccccaac ttccgcgcct tcaacaaaaa cgggtgtcgt
     3721 cacttcatgc ccgcctttga tgcaaggcgt gatcatcggc ccccagcagc ccggtgctgt
     3781 attcgcgaca atttgccctc cgtcctttac aggtccgagc atatcttttg ccggatccaa
     3841 taccccgtca atgaatgagt tcacaaaaag cgtttcaact ggtgtgctta ctgcagtttt
     3901 catcttttct tcccccttct ccatgattct accttcatta taccatatat tgatagaaat
     3961 ttcagaatgc cagtaggaca aaaaagaggc gtacgcctct ttttttcaac tcattatgca
     4021 aatacgattt tttccgtctt ccttcgcttg atagagcgcg acgtctgcct gatggaacag
     4081 tttggtgaat gtccggatcg taccgtttgc gttatcggct agaccgacgc tggcggttaa
     4141 tgaaatgtag atcccgtctt tttcgatttt gattgaccgg acggcttcga gaagattctc
     4201 tgctttatgt tgcgcctcct ccaaggagag gttgcgcagg atgatggcaa attcgtcgcc
     4261 cccgaaacgg cccatcaagt caccgtttcg gataaggctt gagcactctt gaccgatttc
     4321 cttgatgact ttgtcgccga atatatggcc gtacgtatcg ttcaggcttt ttagattgtc
     4381 gagatcgaac gcgatgcaga cgaggctttc gcccgtacgt gcagcttcgg caagccattt
     4441 gtccgtcgtc atttccatat agcttcggtt atacagtccg gtgaggaaat cagtgttggc
     4501 ccggcgttcg attgtcgaaa tccgatgctt cacgcctatt ttgactgccg ccatcgtgac
     4561 ttcgtgctgg cgcagatcgc ttaatagatt aatgtatgct tgctgggctt tgtaaccgcg
     4621 ttgcacgtcc cccatcaatt cacagagcgt gcatctcttt tccaaaatgc ctttcagcaa
     4681 attcgtgtcc ttatagcttt ccgcgatttc tttcgctgtg tcgtatgctt gcaatgcttc
     4741 gggatacatt tcttttttca ttaaataacg tccgtttaaa tcgtgcactt gcgtcttttc
     4801 ccgaatgaat gaatccagga gcggatcatt gaccatttca tcgacaagct gtcccgctgt
     4861 tccgagctga tccaagccga tcaacgcgtt tgcgacgttc agcttcaccc ggaattccaa
     4921 gatcggtgtc cgcggttcat gcaatttcgc catcgccaat cctttttccg ccatcatgag
     4981 cgcgttctca aaatccgatt cgatagcgag aatcatgctg taattgctgt aggcattgct
     5041 gacgatgttg taatgtttga gcttttcacc gagctcgatg gcgcgctcca atgtttcttt
     5101 cgctttttca taatccttat tgtactcatg aagcaggaac aggacgttgc agccattcaa
     5161 tctgtcggct tcatcgcctt tatcggcgca gtattcaata taggcatcaa tgctatggaa
     5221 cgcctcttca attgcaccga tgcagtaaaa ggatgcggca ttgttgatat gggcagtcaa
     5281 aatggatttc gggtcattgg ctgctaaacc ggcttccaat aatcggaagc acgcttcgat
     5341 tgtctcttca tatttccctt ccactctcag cttcaggaca tttcgctgca atgaaaccag
     5401 atcttccttc tccatgccca caacccctat cctattttct tagtgcaata taactgtatg
     5461 atttgtatct ttatttacta ctatagtatc acacagtggt ttattgtgga acttatttat
     5521 tggataaata tgtatggagc cgtataaatc agctccttta tttttgacat tcccccctat
     5581 ttcccggcct ctccttgcag tgcattggcg atttttccac tcggcgtccc agctgcgtca
     5641 agcatgtttg attcggcttt taataaatta ttctcgtact ccttttcgaa tgaagccagg
     5701 agatccactg cggggccaac tcccgcaaat atggctccac tgcttgctac gacggcccct
     5761 gccagtaaaa tcgctgctat ctttttaatc gcaatcactc ccccaagtga tgttattgtc
     5821 tataactata cgggtttccg agtgaataga aaattcaatt ttgttgataa aatacgccaa
     5881 tcaaccttga aaatcgccag tatataaaat gaacccataa gcatcgacag attatattgt
     5941 cagatgctgg gggaacttgg ggaaacattc atttttcgca acaggcataa attgaaaaag
     6001 gaagccggcg tgtcaggccg actcccttca tttcaaatga tttgatcaaa ataagctacc
     6061 gtcttcattt catctccgac attccttaac gaccatgtat cctcgatgta aatctccgcc
     6121 aacttttcga atttgccgcc atgtttcatc gcccattcga gcgctacgac gctttcgtat
     6181 aaatatgcca tccgcttcat gaggcttttc gcttcgaaag tttgaagtgc attatcgaaa
     6241 gcggcaaatt cagtaaggtc ggtggttagt gcatttaatt caaccgacaa cgccttcttc
     6301 atctcgctat catttatgac aacaaggcgg ttgcgcattt catcgatgaa cagcctatga
     6361 gcttcgaatt tattgacgag tcggatgagc tcgagaccga gaatatttgc ggtgccttcc
     6421 cagacggtga gcacttgggc gtcccgcagc aggcgcggcg taacgaaatc ctcgatatag
     6481 ccgttgccgc catgcatttc gattgcttca tgggcgaaat ggattgcctg ctcggccgtc
     6541 tcctttttga tgagcgcgat gaagagcctg ttcaaaatcg tttcgcggcc ggatgcagtc
     6601 ccacccgtca ctcgttcgta caatttgatg acatcaaaga cggtcgccac ttcgacatgg
     6661 agtctagccg ccattttccc
//
LOCUS       DASULI010000048         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_45887, whole
            genome shotgun sequence.
ACCESSION   DASULI010000048 DASULI010000000
VERSION     DASULI010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45887"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /gene="fumC"
                     /locus_tag="VFX34_01045"
     CDS             complement(<1..1237)
                     /gene="fumC"
                     /locus_tag="VFX34_01045"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5563517.1"
                     /translation="MEYRIEHDTFGEIKVPADKLWGAQTQRSKQNFKIGGERIPLGVI
                     QAFAHLKKSAAIASNAFGNLSDAKMKAIQAAAEEVIEGKWDDHFPLVVWQTGSGTQSN
                     MNMNEVLANRGNQLLEEWGEEERLHPNDDVNKSQSSNDTFPTALHVAGVIAVERELLP
                     SLKELKETLGNKSEEFMDIIKIGRTHLQDATPLTLGQEISGWHRMLEKTEKMLNDSVQ
                     SMKELAIGGTAVGTGLNAPKGFGEKVAEEISKSVGIEFTSAENKFHALTSYDEVVYTH
                     GAMKALAADLMKIANDVRWLASGPRSGIGEITIPENEPGSSIMPGKVNPTQSEALTMV
                     VAQVMGNDAAIGFSASQGNFELNVFKPLVIHNFLQSARLLADGCRSFNDNCAVGIEPD
                     RARIDKLLRESLMLVTALNP"
     gene            complement(1339..2268)
                     /locus_tag="VFX34_01050"
     CDS             complement(1339..2268)
                     /locus_tag="VFX34_01050"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5563518.1"
                     /translation="MGQTRNYSQRQTDGQYPVLWMTSGQITLMGRWNNVAEALFSMNE
                     GGSFCAQYNPENTIVETYTLLDGEVEIEYRGNRTKMNIGETLDASCYDKIVTFYGTSD
                     AEILMKMDADFYEQLFFETLTLQNEMDAVSRVDGYTYHHCDRIRQYAIEVWKRMDRPK
                     ESLKLLRWGSYFHDIGKLAIPLEILNKPGKLTPDEWETMKSHSLIGANIMRAHPVEWL
                     KDAAFIVEQHHERYDGKGYPYGLKGDEISIEAAIVSVVDAYDAMTTERVYKLAMTADE
                     AIAELENGKGAQFHPAVVDRFIELFRENKLVRI"
     gene            complement(2379..3650)
                     /locus_tag="VFX34_01055"
     CDS             complement(2379..3650)
                     /locus_tag="VFX34_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5563519.1"
                     /translation="MQFNDQIDFVRQHIKKNKLRVFMTILAATMGTAFLIVLASVGFG
                     IHDTMRKEILDNRLVNQIEVYSSEMDQAKVEELKKRDKVKAVVFRQSVGINQETELDG
                     YTGNNTMTVTDFQEEKKVGFALKEGRLPENKHEVVVGSHFAENLLEQKADEAAPEPST
                     YKEPLIGKQFIYKMGLYEKNELFPEETALTIVGIAKEPAKDWVMDTNIYADTAIMPEL
                     NQIFTHHVEDENSELFYGNVNVYADNLENVTTVSKALKDEGYSVYSIADQLEQLDVFF
                     LAFKAGLVFVGTIAILIASIGIFNTMTMAVTERTREIGVMKAIGASPKLIQRLFLMES
                     AWIGIVGTIIAVAISYGVSLIANMVLPIIVSAALGEEEFASLDITFSIIPWQLVVIAS
                     AISISVAMISGWRPARKATKIDVIQALRQEL"
     gene            complement(3640..>4156)
                     /locus_tag="VFX34_01060"
     CDS             complement(3640..>4156)
                     /locus_tag="VFX34_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563520.1"
                     /translation="DAGIIQVGNVRTSAFNETESAKFRLDHFGFIFQNFQLMPGLTAF
                     ENVELPLKLGGVTKKERRRKVQQLLQLVGLTEVIDHYPNELSGGQQQRVSIARALVTD
                     PPIIFADEPTGSLDSETEQDILLLIQSLNKSRGITFIIITHDDTVAETADRVYRMHDG
                     ELREGGVVHAV"
BASE COUNT          884 a         1090 c          975 g         1207 t
ORIGIN      
        1 gcgggttgag ggccgtcacc agcatcaggc tctcgcgcaa cagcttgtcg atgcgcgccc
       61 ggtccggctc gatgcccacc gcgcagttgt cgttgaagct gcggcacccg tccgcgagga
      121 gccgcgccga ctgcaggaag ttgtggatga cgagcggctt gaagacgttc aattcgaagt
      181 tgccttgact tgccgaaaat ccgattgccg catcattccc catcacttgc gccacgacca
      241 ttgtgagagc ttcactttgt gtcggattga ccttgcccgg catgatggag ctgcctggtt
      301 cattttccgg aatggtgatt tcgccgattc ccgaacgcgg accgcttgct aaccacctga
      361 catcgttcgc gattttcatt aaatccgccg ccaacgcttt catcgcacca tgcgtataga
      421 cgacttcgtc atagcttgtg agggcgtgga atttattttc cgccgatgtg aattcgatgc
      481 cgaccgattt tgaaatttcc tcggctactt tctccccgaa ccctttcggt gcgttcagtc
      541 ctgtgccgac cgcggttccg ccgattgcaa gctccttcat cgattgaacg ctgtcgttca
      601 gcattttctc cgttttttcg agcatgcggt gccagccgct gatttcctgg cccaatgtga
      661 gcggcgtagc gtcttgcaaa tgcgtacgac cgatcttgat gatatccatg aactcttcgg
      721 atttattgcc aagcgtttct ttcagctctt ttaatgaagg aagcaattca cgttccactg
      781 cgattacgcc tgcgacgtgc aaagctgtcg ggaatgtatc gttggagctt tgcgatttat
      841 tcacatcgtc gttcggatga agtcgttcct cttcgcccca ctcctccaac agctggttgc
      901 cgcggttcgc aagcacttcg ttcatattca tattcgattg cgtgccgctg ccggtttgcc
      961 agacgacgag cgggaaatga tcatcccatt tgccttcaat cacttcttcc gccgcagcct
     1021 ggatggcttt catttttgcg tcagatagat tgccgaatgc gttgctcgca attgccgcag
     1081 attttttcaa atgggcgaac gcttggatga cgccaagcgg aatccgttct ccgccgattt
     1141 taaagttctg tttgcttcgc tgtgtttgcg cgccccataa cttatccgcc ggtactttga
     1201 tttctccaaa tgtgtcatgc tcgatacgat attccatgtt ccctccgcct ttcacatatg
     1261 aataattgaa aaccctcctt ctatcatgca cctttttcga gttgaaataa aatgaaaaga
     1321 cccggttttc cgagtctttc agattcttac aagtttattt tcacgaaata attctataaa
     1381 tctatccact acagcagggt ggaattgagc cccttttcca ttttccagct ctgcaatcgc
     1441 ttcgtccgcc gtcattgcca gtttgtaaac ccgctccgtt gtcatggcgt cataggcgtc
     1501 gacgacggag acgatggctg cctcgatgga gatttcgtcc cccttcaatc cataggggta
     1561 ccctttgcca tcatatcgct cgtggtgctg ctcaacgatg aacgctgcat ctttcagcca
     1621 ttccaccggg tgggcgcgca tgatgttggc gccgatcagg gaatgcgatt tcatcgtttc
     1681 ccattcgtcc ggcgtgagct ttccgggttt attcaaaatc tcgagaggaa ttgcaagctt
     1741 gccgatatcg tggaagtagg aaccccatcg cagcaatttg aggctttctt tcggcctgtc
     1801 catccgtttc cacacttcga tggcgtattg cctgatccgg tcgcaatggt gatatgtata
     1861 accgtcgaca cgtgagacgg catccatctc gttttgcaat gtcagcgttt cgaaaaataa
     1921 ttgttcatag aagtccgcat ccattttcat gaggatttcc gcatctgatg taccgtaaaa
     1981 cgtgacaatc ttgtcgtaac aggatgcgtc caacgtttcc ccgatattca ttttcgtacg
     2041 attgccgcgg tattcgattt cgacttcgcc gtccaatagc gtatacgttt cgacaatggt
     2101 attctccggg ttgtattggg cgcagaacga tcccccttca ttcattgaaa agagggcttc
     2161 cgcaacatta ttccaacgtc ccataagtgt gatttgtccg gatgtcatcc ataaaacggg
     2221 gtactggcca tcagtttgtc gttgcgagta attccgggtt tgtcccatag gcatcctccg
     2281 tcaagcagat ttcgacttct tccgattgtt tctcccatta taacggaaaa tacccgtgac
     2341 ttctacaact cttttgaata aatggaatat actttctttt ataactcctg tctcaatgct
     2401 tgaatgacat caattttggt tgctttacga gcggggcgcc aacctgaaat catagcgact
     2461 gaaatgctga ttgcggatgc gatgacgacg agttgccatg gaatgatgga aaacgtgata
     2521 tccaaacttg cgaactcctc ttccccgagc gccgcagata cgatgatcgg caataccata
     2581 ttggcgatga ggctgacgcc gtatgatatg gcgaccgcga tgatggttcc tactatgcca
     2641 atccatgcgc tctccataag gaacagcctt tgaatcagtt tcggacttgc cccgatcgct
     2701 ttcatgacgc cgatttcgcg tgtccgttct gttactgcca ttgtcatcgt gttgaagatg
     2761 ccaatcgacg cgattaagat cgcgatggtg ccgacgaaga cgagacctgc tttgaatgcc
     2821 aggaagaaaa cgtcgagttg ctcaagctgg tcggcaatcg aataaacgga atatccttcg
     2881 tctttgagcg ccttgcttac ggttgtgacg ttttccagat tatcggcata cacattgacg
     2941 ttcccataaa atagttcact attttcatcc tcaacgtggt gggtgaaaat ttgattcaac
     3001 tcgggcatga ttgccgtgtc cgcataaatg ttcgtgtcca tgacccaatc cttcgcaggc
     3061 tcttttgcga tgccgacgat ggttaatgct gtttcttccg ggaatagttc atttttttca
     3121 tacaatccca ttttgtatat gaactgtttg ccgattagcg gctccttgta tgttgaaggt
     3181 tccggcgctg cttcatccgc tttctgctcg agcaggtttt ccgcaaaatg acttccgaca
     3241 acgacttcat gtttattctc cggcagccgg ccttctttta gggcgaagcc cactttcttt
     3301 tcttcttgga aatctgtaac ggtcatcgta ttgttgccgg tatatccgtc cagttccgtt
     3361 tcctggttga tgcctacaga ctggcggaag acgaccgcct tcactttgtc acgctttttc
     3421 agctcctcca ctttcgcctg gtccatctcg ctggagtaaa cttcaatctg gttcaccaac
     3481 cggttatcga gtatttcttt tcgcatcgta tcgtggatgc cgaagcctac ggatgcaagg
     3541 acgatgagaa atgccgtccc cattgttgca gctaagattg tcataaagac gcgcagtttg
     3601 ttctttttaa tatgctggcg gacaaaatcg atttgatcgt taaactgcat gaacgacacc
     3661 cccttctctt aattcaccgt cgtgcatgcg gtacacacgg tcggctgttt ccgccaccgt
     3721 gtcgtcatgc gtaatgatga taaatgtgat gccacgggat ttatttagtg attggatgag
     3781 gagaaggatg tcctgctccg tctctgagtc gaggctcccg gtaggctcat ccgcaaagat
     3841 gatcggtgga tcggtgacaa gcgcacgcgc aatgctcact cgctgttgct gaccacccga
     3901 taattcattc ggataatggt ctatcacttc tgtgagccca acaagctgaa gcagctgctg
     3961 gacttttcgc cggcgttcct ttttagtgac gccccctagt ttcagaggca actcgacatt
     4021 ttcgaatgcc gtgagacccg gcatgagttg gaagttttga aaaatgaaac cgaaatgatc
     4081 gaggcggaac ttcgcgcttt ccgtctcatt gaacgcagaa gtccgaacgt tgccgacttg
     4141 aatgatgccg gcatcc
//
LOCUS       DASULI010000049         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_308, whole
            genome shotgun sequence.
ACCESSION   DASULI010000049 DASULI010000000
VERSION     DASULI010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFX34_01065"
     CDS             <1..425
                     /locus_tag="VFX34_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEX5563521.1"
                     /translation="VDLARVQKACQELPESMPLETYLRVLTIHELGHAMDRTALLDSL
                     DRTKEIILMKKQAAAEKRPTDLPFMTMVMEEHETDIAFEETAWANAEILNSFFEIVDG
                     DSFEKVKAHSLSTYRAAYGADLEIYNGFIRTQEELLSI"
     gene            complement(470..1639)
                     /locus_tag="VFX34_01070"
     CDS             complement(470..1639)
                     /locus_tag="VFX34_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5563522.1"
                     /translation="MQFEELIYEIDRRIQEGTFIHGTISQPRYKSTGLQKVKLKPVEI
                     KKEVHIQFEYHYERILKHENIKAPDVQPVLRQLLEQFKQMQVEFTDEKVHIQLTKKFK
                     VSWKGEKTTTTKKVDLSHNRKKNYLLDDETPYPFLIRLGVQSPEGKVKKQKYDKFRQI
                     NRFVEFIDDALVHLPKDRPVRILDFGSGKSYLTFALYHYLHIEKGLDIRVTGLDLKKE
                     VIEECNLIAKDLGYEQLEFLVGDINDYNEETSVDMVVTLHACDVATDMALARAVKWGA
                     EVILSVPCCQHELFSQIQSPALDVMLQHGLIKERFSALATDSIRAELLSLVGYDAQLL
                     EFIDMEHTPKNILIRAYKTGKKPSAESVAKYEAFRDLLNAKPFLERELKEAGYLQ"
     gene            complement(2000..2731)
                     /gene="nfsA"
                     /locus_tag="VFX34_01075"
     CDS             complement(2000..2731)
                     /gene="nfsA"
                     /locus_tag="VFX34_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HEX5563523.1"
                     /translation="MVIELLTSHASVRKYKNIPLSKEDVRELVHAGQHAASSHFVQAY
                     SVIHVTDMEKREQLAELSKNKRQFLSAGAVLIFCADYCRLAKAAALHDKEIDYSHAEN
                     VLVGSIDVALFAQNVAIAAESKGYGICYIGGIRNAPEEISELLELPDGVAPMFAMSIG
                     VPDEANEVKPRLPVEAIIHENSYDAAKYTEILPAYDKTMHDYYLNRGSNQKDTAWSVQ
                     MADFLAEPRRTYMKEFLAKRGYTFK"
     gene            2821..>3067
                     /locus_tag="VFX34_01080"
     CDS             2821..>3067
                     /locus_tag="VFX34_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX5563524.1"
                     /translation="MDWFEGISLTTTLLVLILVIIPISVAIYLYFYDKRQKQHAILRN
                     YPVLGRMRYVLEKTGPELRQYLFNADHEGKPFNREEYL"
BASE COUNT          737 a          707 c          732 g          891 t
ORIGIN      
        1 ttgtcgacct tgcccgtgta caaaaagctt gccaagaact ccccgaatcg atgccattgg
       61 aaacctatct tcgcgtactg accatccacg agcttggaca tgcgatggac cgcacagctt
      121 tgctcgactc acttgaccgt acaaaagaaa ttatccttat gaaaaaacaa gccgctgcag
      181 aaaaacggcc gacagatctg ccgttcatga cgatggtgat ggaggaacat gaaactgata
      241 tcgccttcga ggaaactgca tgggccaatg ccgaaatttt gaacagcttt ttcgaaattg
      301 tcgatggaga cagctttgag aaagtgaaag cgcatagcct ttcaacatat cgtgccgcgt
      361 atggggccga cttggaaatc tacaatggat tcattcgtac gcaagaggaa ttattatcta
      421 tataaaatca aagaaaccgc cagcctgatg caatcagggg cggttttatt tattgtagat
      481 acccggcttc ttttaattcc cgttctaaaa acggttttgc gttcagcaag tcacggaacg
      541 cttcgtactt tgcaacggat tctgctgacg gtttcttgcc cgtcttataa gcgcgaatca
      601 gtatattctt cggcgtatgc tccatatcga tgaactcgag cagctgcgcg tcatagccga
      661 caagcgatag gagttcagcg cgaatggagt ctgtcgcaag agctgagaag cgctctttga
      721 tcagcccatg ctgcagcatg acgtcaagcg caggcgattg gatttgcgag aacagctcgt
      781 gctggcagca aggtacgctc aaaatgactt cggctcccca tttgacagca cgggcgagtg
      841 ccatatctgt tgcaacatcg catgcatgca aagtgacgac catgtcgacg gaggtttctt
      901 cgttatagtc gttaatatcc ccgacgagaa attcaagttg ttcatagcct aggtctttgg
      961 cgatcagatt acattcttca atgacttcct ttttcaaatc gagccccgtt acccggatat
     1021 ccagcccttt ttcgatatgc aaataatgat agagggcaaa cgtcaaatac gatttgccgg
     1081 aaccgaagtc caaaatgcgg acgggtcggt ctttcggcaa atggacgagt gcatcatcga
     1141 taaattcaac gaagcggttg atttgccgga acttatcgta tttttgcttc ttcacctttc
     1201 cttcgggcga ttgaacacca aggcggatga ggaaaggata gggagtttca tcgtccaata
     1261 gataattctt tttccggtta tgcgacaagt cgactttctt cgttgtagtc gtcttctcgc
     1321 ctttccacga aactttgaac ttcttcgtca gctggatatg gactttctca tcggtgaatt
     1381 ccacttgcat ctgcttgaat tgctccaata attgacggag aacaggctgg acatctggag
     1441 cctttatatt ttcatgtttc aaaatgcgtt cgtagtgata ttcaaattga atatggactt
     1501 ctttcttgat ttccaccggc ttcagtttca ctttctgcaa gccggtcgat ttgtagcggg
     1561 gctgactgat cgtcccgtgg atgaacgtcc cttcctggat tcggcggtcg atttcataga
     1621 ttagttcttc aaactgcata atgctggacc tctcttcgta caaatatgta cttataagaa
     1681 tacaacaagg gactggaaag atgtgctcct aacgcttcgc ttttgctctc aacgctatgc
     1741 ttttgttcgc agtgcccgtt ctacgtgacg ggcagtactc gcaaaagccg ttcttcgtaa
     1801 cggctttgcc aatcccctga agtgaatccc gtttatgcgt ggaaatttca atttatgcgt
     1861 gctttagacg atttatgagt ttttcggcaa gtttatgcgt agaaaaaccg ttttatgcgc
     1921 ttttggggtg gtttatgcgt gaataaatcg atttatgcgc ttttggaacg ttttatgcgt
     1981 gaatcaatca tacagtcttt catttaaacg tatatccgcg cttcgccaag aattctttca
     2041 tgtatgtccg tcttggttca gcgaggaagt cggccatttg cacggaccat gctgtatcct
     2101 tttggttcga cccgcgattg aggtaatagt cgtgcatcgt tttgtcgtag gcgggcagaa
     2161 tctcggtgta tttggcagcg tcatagctgt tttcatggat gatcgcctcg actggcaatc
     2221 tcggcttcac ttcgttcgct tcgtccggga cgccgatcga catggcgaac atcggcgcta
     2281 cgccgtccgg aagttctaat agctcgctga tttcctccgg ggcgttgcgg atgcctccga
     2341 tatagcagat gccgtatcct tttgactctg ccgcaatcgc gacattttgg gcaaagagcg
     2401 caacgtcgat ggagcctaca aggacgtttt ctgcatggga ataatcgatt tccttatcat
     2461 gcaatgcggc ggcttttgcc aatctgcagt aatctgcaca gaagatcagt accgctccgg
     2521 cagaaaggaa ctgcctttta ttcttagaca gttcggccag ctgctcccgt ttttccatgt
     2581 cggttacgtg gatgacggag taggcttgta cgaaatggga gcttgccgcg tgctggcctg
     2641 catggaccag ttcccgtacg tcctcttttg aaaggggaat gttcttgtat ttgcgtacgg
     2701 aagcgtgtga tgtcaataat tcgatgacca tatggttccc tccagaatat ttcgttattc
     2761 tatattgtgc gaatatgttc atgttacact actcataacg cttagaaaag gggtgtcctc
     2821 atggattggt ttgaaggtat atcgttgacg acaacattgc tcgtacttat tttagtcatc
     2881 attcctattt cggtcgctat ctatctttac ttttacgaca aacggcagaa gcagcatgcg
     2941 attctccgga actatcctgt cctgggacgg atgcgatatg tgttggaaaa gacaggaccc
     3001 gagctgcggc aatatttatt caatgcggac catgaaggga agcctttcaa ccgggaagaa
     3061 tacttgc
//
LOCUS       DASULI010000050        10753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1929542,
            whole genome shotgun sequence.
ACCESSION   DASULI010000050 DASULI010000000
VERSION     DASULI010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10753
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929542"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFX34_01085"
     CDS             <1..685
                     /locus_tag="VFX34_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5563525.1"
                     /translation="GRMTEDEKSEVLGRITKSLDLQDAHDVDIVIEAAIENMDIKKSI
                     FAKLDGIAPKHAILATNTSSLPITEIAAVTNRPQNVIGMHYMNPVPVMKLVEIIRGLA
                     TSDEVYQAVEDMTRKLSKTPVEVNDFPGFVANRVLMPMINEAIYTLYEGVASKEAIDE
                     VMKLGMNHPMGPLQLADFIGLDTCLYIMETLHEGFGDSKYRPCPLLRKYVNAGWLGKK
                     SGRGFYEYS"
     gene            880..2016
                     /locus_tag="VFX34_01090"
     CDS             880..2016
                     /locus_tag="VFX34_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5563526.1"
                     /translation="MDLQFTEEQQMMRTMVRDFAKSEIEPFIPRMEAGEFPREILAKM
                     GELGLMGITVPEKYGGSDMDFLSYIIAIHELSKVSAVMGVILSVHTSVGTNPIMYFGN
                     EEQKNRYLPKMASGEYLGAFCLTEPSAGSDAGSLKTRAVKKDDHYVLNGSKVFITNGG
                     EADVYIVFASTDPSQGTYGITGFIVDKDTPGLIIGKDEEKMGLHGSRTVQLTFEDMKV
                     PVENRLGEEGEGFKIAMANLDVGRIGIAAQALGIGEAALEAATAYAKERVQFGKPIVA
                     NQGVGFKLADMATASEAARLLVYRAAQLRAEGKPCGQEASMAKLFASQTAMDNAIEAV
                     QIFGGYGYTEDYPVERYFRDAKVTQIYEGTSEIQRIVINKHLTK"
     gene            2212..3351
                     /locus_tag="VFX34_01095"
     CDS             2212..3351
                     /locus_tag="VFX34_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5563527.1"
                     /translation="MDFRLSEEHEMIRKMVRDFAEKDVAPTAAERDEEERFDMDIWHK
                     MAELGLTGIPWPEEYGGIGSDYLAYCIAVEELSRVCASTGVTLSAHTSLAGWPVYKFG
                     TEEQKQKYLRPMAEGTKIGAYGLTEPGSGSDAGGMRTTAKLDGDDYILNGSKIFITNG
                     GIADIYIVFAVTDPESKHKGTSAFIVEKDFPGFSVGKKEKKLGIRSSPTTEIMFDNCR
                     VPKENMLGAEGEGFIVAMKTLDGGRNGIAAQAVGIAQGALDAAVDYAKERVQFGKPIA
                     ANQGVGFKLADMATATEASRLLTYQAAWLESNDLPYGKASAMAKLMAGDTAMKVTTEA
                     VQVYGGYGYTKDYPVERFMRDAKITQIYEGTQEIQRLVISRMLTK"
     gene            3365..3991
                     /locus_tag="VFX34_01100"
     CDS             3365..3991
                     /locus_tag="VFX34_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5563528.1"
                     /translation="MQLKREVKSSVKDESLIEKRREQIIDGAVQLFKEKGFHRATTRE
                     IAKAAGFSIGTLYEYIRTKEDVLYLVCDSIYNEVQSRLSSLMDTEGNVEGLRMAIDSY
                     FHLIDDMSDEFVVMYQESKSLPKDALQYVLKKELEMVALFKNLLNACVRSGELRIDEG
                     AVDMAAHHIVVQGQMWAFRRWALRGNYTIEQFISYQTDQLFKGIVGDQ"
     gene            4324..7581
                     /gene="icmF"
                     /locus_tag="VFX34_01105"
     CDS             4324..7581
                     /gene="icmF"
                     /locus_tag="VFX34_01105"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019380587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEX5563529.1"
                     /translation="MVSSEIYKPKHHVRFVTASSLFDGHDASINIMRRILQSTGAEVI
                     HLGHNRSVEEVVNAAIQEDVQGIAISSYQGGHVEYFKYMYDLLQEKGAPHIRIYGGGG
                     GVILPKEIKELHDYGIAWIFSPEDGRKMGLQGMINRMVEECDFLTEVEDEMANLEKVG
                     TDNPEVLANLITYAEEMYDKENPAAINLIKKARELSKNTPVLGITGTGGAGKSSLTDE
                     LIRRFLRELPDKKVAILSIDPTKQKTGGALLGDRIRMNAIFNKRVFMRSLATRGSRSE
                     LSGAIKDVLDVVKTAGFDLIIVETSGIGQGDAEITEVSDVSMYVMTSEFGAPTQLEKI
                     DMIDFADLIVINKFERKGSEDALSQVQKQYQRSHLLFDKDLDTMPVYGTIASQFNDKG
                     TNSLFAALVSKLNEKCGLDWETSYADFVKTQKQNVIIPTDRVHYLREIASTIRDYHKK
                     SAQQVDLARRLFQLEGAIEAVNEQSPDNALTASLESLANGVREELSAESKRILNNWET
                     LKESYAGDEFVTKIRDKELRTLLTTTSLSGLKIPKVSLPKFKDYGEILRWVYKENVPG
                     SFPYTAGVFPFKREGEDPKRQFAGEGTPERTNRRFHYLSKDDDAKRLSTAFDSVTLYG
                     EDPDERPDIYGKVGESGVSICTLDDMKKLYDGFDLCAPSTSVSMTINGPAPIILAMFM
                     NTAVDQQVKLKEEELGRVLTVEEFTEVRNATLQVVRGTVQADILKEDQGQNTCIFSTE
                     FALRMMGDIQQYFIDQKVRNYYSVSISGYHIAEAGANPISQLAFTLANGFTYVEYYLS
                     RGMNIDDFAPNLSFFFSNGLDPEYTVIGRVARRIWAVAMREKYGANERSQKLKYHVQT
                     SGRSLHAQEIDFNDIRTTLQALMALQDNCNSLHTNAYDEAITTPTEESVRRAMAIQMI
                     ITKEHGLSKNENPLQGSFIIEELTDLVEESVLQEFDRLNDRGGVLGSMETQYQRGKIQ
                     EESMLYEMKKHSGELPIIGVNTYLNPNPPSEEDIDNMEIARATKEEKETQITNLRAFQ
                     SQHSDESAEALRKLQQVAVTGGNIFAELMETVKVASLGQITNALYEVGGQYRRNM"
     gene            7725..7889
                     /locus_tag="VFX34_01110"
     CDS             7725..7889
                     /locus_tag="VFX34_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563530.1"
                     /translation="MKLYHHVLIIIVIIVLIFSLYGVGYGAVPFLLAALYLGCLSVKE
                     FQRFKKSRRW"
     gene            7956..8531
                     /gene="rpoE"
                     /locus_tag="VFX34_01115"
     CDS             7956..8531
                     /gene="rpoE"
                     /locus_tag="VFX34_01115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEX5563531.1"
                     /translation="MNLHELTKEELLEESMIDIAYAILTERREALTLEQLMNEIRELT
                     GMSESQMKDRLLQFYTDMNIDGRFLAINNNEWGLREWYPVDQIEEETAPVVKVRKKKK
                     KKAYDDDEEEDEEDEEEIDEEELFDEEFDELDDDEDDLDEEEEEEEEIIEIEEDLIDD
                     EEGLEIVPDDELDIDDEDEEDEEEEEEEDLL"
     gene            8699..10309
                     /locus_tag="VFX34_01120"
     CDS             8699..10309
                     /locus_tag="VFX34_01120"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5563532.1"
                     /translation="MTKYIFVTGGVVSSLGKGINAASLGRLLKARGLQVTNQKFDPYI
                     NLDPRMMSPYQHGEVFVTEDGAETDLDIGHYERFIDIKLNKYSNVTMGKVYSSVLRKE
                     RRGDYKGATVQVIPHITNEIKSLVKRAGQETNADVVITEIGGSVGDIESLPYLEAIRQ
                     MKTDLGKDDVMYIHNTLIPYLHAAGEMKTKPTQHSVKELRGLGIQPNMIVVRSEYPVP
                     QEMKDKIALFCNIKPEEVIEALDAETLYEVPIRLHAQNMDKIVVDFLGLETKEPDLTE
                     IEELVNRVRNLSRKVKIALVGKYVELQDAYISAVEAFRHAGYEFDTDIEIDWINSEEV
                     TKENVAETLEEADGIFVPGGFGDRGIDGKMEAISYARENGIPFFGVGLGMQLAAVEFA
                     RDVLGIKDAHSVEFDSETENAIIENNRDFEDRSDIEQASGDMRLGAHPCKLKEGTKAQ
                     EAYNDELVYERHRHRFEFNNIYREQFENAGMIVAGESPDGHLVEIIELKDHPFFIGTQ
                     FHPEFASRPTRPTPLIREFIRAALAHQG"
     gene            complement(10627..10740)
                     /locus_tag="VFX34_01125"
     CDS             complement(10627..10740)
                     /locus_tag="VFX34_01125"
                     /inference="COORDINATES: protein motif:HMM:NF022200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 family protein"
                     /protein_id="HEX5563533.1"
                     /translation="MPGEELGSRIVQPHLMAALFLYEAVKMAYDEMVSEED"
BASE COUNT         3020 a         2591 c         2896 g         2246 t
ORIGIN      
        1 aggccgcatg acggaagatg aaaaatcgga agtcctcggg cgcattacga aatcactcga
       61 tttgcaggac gcacatgacg tagacatcgt catcgaggcg gcgattgaaa acatggacat
      121 caaaaagtcg atcttcgcga aactcgatgg catcgcgccg aagcatgcga ttttggcgac
      181 aaatacatca tcccttccga ttacagaaat cgcggcagtg acaaaccgtc cgcaaaacgt
      241 cattggcatg cactatatga acccggtgcc tgtcatgaaa ctcgtcgaaa tcatccgcgg
      301 actcgcgact tccgacgaag tgtatcaggc ggtcgaggac atgactcgta aactatcgaa
      361 gacgccggtc gaagtgaacg acttccctgg attcgtcgca aaccgcgtcc ttatgccaat
      421 gatcaacgaa gcaatctaca cgctatacga aggcgtcgcc tcgaaggaag cgatcgacga
      481 agtgatgaag ctcggcatga accacccgat ggggccgctt caacttgcgg atttcatcgg
      541 actcgataca tgtctgtaca tcatggagac gctccacgaa ggcttcggcg actcgaaata
      601 ccgcccttgc ccattgctga ggaagtacgt caacgcaggc tggctcggga agaaatcagg
      661 tcgcggcttc tatgaatatt cataatgtgt ggtctaaggt cattaaatga cgataacttg
      721 cgaaagttga ccgataaaat tgtgaaactg accgatatcc gatcgaagtt gaccgataaa
      781 attgtgaact tgatcgataa tccaccggat ttgaccgata gaattttgta cttgattgat
      841 aatgagcgat taacacgtta agtaaaggag ttaattccaa tggatttaca attcaccgaa
      901 gaacagcaga tgatgcgcac aatggtgagg gactttgcga aatcggaaat cgagccgttc
      961 attccacgga tggaggcggg ggagttcccg cgtgaaatcc ttgcgaaaat gggcgaactc
     1021 ggcttgatgg gcatcaccgt ccccgaaaaa tacggcggct ccgatatgga tttcctttcc
     1081 tatatcatcg ccatccacga gctgtcgaaa gtgagcgcgg tcatgggggt catcctttcc
     1141 gtccatacat ccgtcggcac gaacccgatc atgtatttcg ggaatgaaga gcagaaaaat
     1201 cgctacttgc cgaaaatggc atcgggcgaa tatttagggg ccttctgcct gacggagccg
     1261 agcgcagggt ccgacgcggg ctcgttgaag acgagagccg tgaaaaagga cgatcattac
     1321 gtgttgaacg gctcgaaggt gttcattacg aacggcgggg aggcggatgt atacatcgtt
     1381 ttcgcgtcaa ccgatccgtc gcaagggacg tacggcatta caggcttcat cgtcgataag
     1441 gatacaccgg gcctcatcat cggaaaagac gaggaaaaga tgggccttca cggatcgcga
     1501 acggtgcagc taacgtttga agacatgaaa gtaccagtcg agaaccgcct cggtgaagaa
     1561 ggggaaggct tcaaaatcgc aatggcgaac cttgacgtcg gtcggatcgg catcgcggcg
     1621 caggcgctcg gaatcggcga agcggctctc gaagcagcta ccgcgtatgc gaaggaacgc
     1681 gtccagttcg ggaaaccgat cgttgcgaac caaggcgtcg gcttcaagct tgcggacatg
     1741 gcgactgcct ccgaagcggc aagactcctc gtctaccgcg cagcccagct tcgcgctgaa
     1801 ggcaagccat gcggccaaga ggcatccatg gcgaagctgt tcgcttcgca aacagcaatg
     1861 gacaacgcaa tcgaagcggt ccaaatcttc ggcggctatg gctacaccga agactacccg
     1921 gtcgagcgct acttccgcga cgccaaagtg acccaaatct acgaaggcac aagcgaaatc
     1981 caacgcatcg tcatcaacaa acacctcaca aagtgaattg tgtgagaatt gtgtgggtgc
     2041 ctgtgcaagg tgcatttatg acaattcaac taagtgaata tatattcatg tttaaagcta
     2101 gggaatgttg ttgcaacagc gattgcattt caaaataata caattgtagt taataaactg
     2161 aattgtatgg tgcacaggca ccgtacaaga atctctgagg aggaagttga catggatttt
     2221 agattatcgg aagagcatga aatgattcgg aaaatggtgc gtgattttgc ggagaaggat
     2281 gtggcgccga cagctgcgga gcgcgatgag gaagagcgct ttgatatgga tatatggcat
     2341 aagatggccg agctcgggtt gacggggatt ccttggcctg aggagtatgg cggcatcggc
     2401 agcgattatc ttgcgtattg catcgcggtg gaagagttgt ctcgcgtatg tgcatccaca
     2461 ggagttacat tatccgcgca cacgtcgcta gctggatggc cggtatacaa atttggtacg
     2521 gaagagcaaa agcagaaata ccttcgtcca atggcggaag gaacgaaaat cggcgcgtac
     2581 ggcttgactg agccgggctc cggttcagat gctggcggca tgcgcacgac tgcgaagctt
     2641 gatggagacg actatattct caacggctcg aagatcttca ttacaaacgg cgggattgcc
     2701 gacatttaca tcgtgttcgc cgtgacggac cctgaatcga agcataaagg cacgagcgca
     2761 ttcatcgttg aaaaggactt cccaggattc tcggtcggga agaaggagaa gaagcttggc
     2821 atccgttcat caccgacgac ggaaatcatg ttcgataact gccgtgtgcc gaaggaaaac
     2881 atgttgggtg cggaaggcga agggttcatt gtcgcaatga agacgttgga cggcggccgg
     2941 aacgggattg ctgcccaagc ggtcggtatc gctcaaggtg cgcttgacgc ggcggtcgat
     3001 tatgcgaaag agcgcgtcca attcggcaag ccgatcgcgg cgaaccaagg cgtcggcttc
     3061 aagctggcgg atatggcgac ggcgacggaa gcttcacgat tactcacata ccaggcggca
     3121 tggctagaat cgaacgactt gccgtatgga aaagcatccg cgatggcgaa actgatggca
     3181 ggggatacgg cgatgaaagt gacgacggaa gcggtccagg tgtatggcgg atacggctac
     3241 acgaaggact atccggtcga gcggttcatg cgcgacgcga agattacgca aatctacgaa
     3301 ggaacgcagg aaattcaacg tctcgtcatc tcccgcatgc tgacaaaata atcgggggtg
     3361 cacaatgcaa ttgaaacgcg aagtgaaatc gtcggtcaaa gatgaaagcc taattgaaaa
     3421 gaggcgtgag cagatcatcg atggggccgt ccagctattc aaggaaaaag ggttccaccg
     3481 ggcgacgaca agggagatcg ccaaagctgc aggctttagc ataggcacgc tctatgaata
     3541 tatccggacg aaagaggacg tcctctatct cgtctgtgac agcatataca atgaagttca
     3601 gagccgcctg tcgtcattga tggacacgga agggaatgtc gaagggctcc ggatggcgat
     3661 cgattcgtat ttccacctga tcgacgacat gtccgacgaa ttcgttgtca tgtaccagga
     3721 atcgaaatca ttgccgaagg acgcgctcca atacgtcctc aaaaaagaac tggaaatggt
     3781 cgccttattt aagaacctgt tgaatgcatg cgtcagatcg ggcgaattgc ggatagacga
     3841 gggagcggtc gacatggccg cacatcacat cgtcgtccaa ggccaaatgt gggcattccg
     3901 cagatgggct ttacgcggga actacactat cgagcaattc atcagctacc agaccgacca
     3961 actgttcaaa ggaatcgtcg gagatcaata aattgtgtgg gtgcctgtgc atggtgcatt
     4021 tatgacaatt taaccgtttg cataaaaatg catcatcaga tatgcgaaag gttgtaaaaa
     4081 tcacctttcc gtttctgatt tatacaattg tagctaataa actgaatagt gtcggtgcct
     4141 gtgcacgaca catttatgac aattcaacaa taggcataaa aatgcatccg gaacagcaga
     4201 gaaggttgtc atagtcactt gctcgtttca gttctatgca attgtagtta ataaactgaa
     4261 ttgtgtggtg cacaggcacc gcaaaaaaga agaatgaagg ggtgtgcggg agtggttacg
     4321 aaagtggtta gtagtgagat ttataagccg aagcatcatg tgcgttttgt gacggcgtcg
     4381 agtttgtttg atgggcatga cgcgtcgatt aatattatgc gccgaatcct ccagtcaacg
     4441 ggggcggaag tgatccatct ggggcataac cgttctgtcg aagaagtcgt caatgcagca
     4501 attcaggagg atgtgcaagg gatcgccatt tcgtcgtacc agggcgggca tgtcgagtac
     4561 ttcaaatata tgtatgacct gctgcaggag aaaggtgctc cgcatatccg gatttacggc
     4621 ggcggtggcg gcgtcatcct gccgaaggaa atcaaggaat tgcatgatta cggcatcgct
     4681 tggattttct cgccggagga cggccgcaag atggggctgc aagggatgat caaccggatg
     4741 gtggaggaat gcgatttcct taccgaagtg gaagatgaga tggccaacct tgaaaaagtc
     4801 ggcacggaca atccggaagt gctagccaat ttgatcacgt acgcggaaga aatgtatgac
     4861 aaggaaaacc cggccgcgat taatctaatc aaaaaagcaa gggaactatc gaagaacacg
     4921 cctgtcctcg gaatcaccgg tacaggggga gcggggaaat cttcgctcac cgatgaactg
     4981 atccgcaggt tccttcgtga attgccggac aagaaagtcg ccatcctctc gatcgacccg
     5041 acgaagcaga agacgggcgg cgcgctgctc ggcgaccgga tccgcatgaa cgcgatcttc
     5101 aacaagcgcg tcttcatgcg cagtctcgca acacgcggtt cacgttccga actatcaggt
     5161 gccatcaagg acgtgctcga tgtcgtgaaa acggcgggct tcgatctgat catcgttgaa
     5221 acgagcggaa tcgggcaagg cgacgcggaa atcacggaag tgtccgacgt gtcgatgtat
     5281 gtcatgacga gcgaattcgg tgccccgacg cagcttgaga aaatcgacat gatcgacttc
     5341 gcagacctga tcgtcatcaa taaattcgag cggaaaggat ccgaggacgc gctcagccaa
     5401 gtgcagaagc agtaccagcg gagccacctc ctgttcgaca aggacctcga tacgatgccg
     5461 gtctacggca cgatcgcgag ccagttcaac gacaaaggga cgaactcgct gttcgctgcg
     5521 ctcgtttcga agttgaatga aaaatgcggc ctcgactggg agacgtccta tgccgatttc
     5581 gtgaagacgc aaaagcagaa cgtcatcatc ccgaccgacc gcgtccatta tttacgtgaa
     5641 atcgcaagca cgatccgcga ctaccataag aaatctgcac agcaagtcga ccttgcccgc
     5701 cgtttattcc aactcgaggg tgcgattgag gctgtgaatg aacaatcgcc ggacaacgcg
     5761 ctgaccgctt cactagaatc gctggcaaac ggtgtccgtg aagagctgtc cgcggaatca
     5821 aagcggattc tgaacaactg ggaaactttg aaagaatcgt atgcaggtga tgaattcgtc
     5881 acgaaaatcc gtgataagga gcttcgaaca ttactcacga caaccagttt gtcagggttg
     5941 aagattccga aagtgtcctt gccgaaattc aaggactacg gcgaaatcct tcgctgggta
     6001 tacaaagaga atgtcccggg ctcattccca tatacggctg gcgttttccc gttcaagcgg
     6061 gaaggtgaag acccgaaacg ccaattcgcg ggcgagggca cgccggaacg gacaaatcgc
     6121 cgcttccatt acctgtccaa agatgacgat gcaaaacggt tgtccacggc attcgactcg
     6181 gtgacgttat acggcgaaga cccggacgag cgcccggaca tttacggaaa agtcggggaa
     6241 tcgggcgtaa gcatctgtac actcgatgac atgaaaaagc tgtatgacgg ctttgacctt
     6301 tgcgcgccat cgacgtccgt ttcgatgacg atcaacggtc ccgcgccgat catccttgca
     6361 atgttcatga atacggcggt cgaccagcaa gtgaaactta aagaagaaga acttggccgc
     6421 gtgcttacgg tcgaggaatt cacggaagtc cgaaatgcga cactccaagt cgtccgcgga
     6481 acggtgcaag cggatatcct gaaggaagac caagggcaga atacgtgcat tttctcgacg
     6541 gaattcgcgc ttcgcatgat gggcgacatc cagcagtatt tcatcgacca aaaagtgcgg
     6601 aattactact ccgtctcgat ttcgggctac catatcgcgg aagcgggcgc caatccgatt
     6661 tcgcaactcg cgttcacgct cgcgaacggc ttcacgtatg tcgagtatta cttgagccgc
     6721 ggaatgaaca tcgatgattt cgctccgaat ctgtcgttct tcttctcgaa cggtcttgac
     6781 ccggaatata cggtcatcgg gcgtgttgcc cgccgaatct gggcggtcgc gatgcgcgag
     6841 aaatacggcg cgaatgaacg gagccagaag ctgaagtacc acgtacagac atccggccgg
     6901 agcctgcacg cgcaggaaat cgacttcaac gacatccgga cgacgctgca ggcgcttatg
     6961 gcattgcagg acaactgcaa ctcgctccat acgaacgcct acgacgaagc gattacgacg
     7021 cctacggaag aatcggtccg ccgtgcgatg gcgatccaga tgatcatcac gaaggagcat
     7081 ggtttatcga aaaatgaaaa tccgctccaa ggttctttca ttattgaaga actgacggac
     7141 ctcgtcgaag aatccgtcct tcaggagttc gaccgtctga acgaccgcgg cggcgtgctc
     7201 ggctcgatgg agacgcaata ccagcgcggc aaaatccagg aagaatcgat gctctacgaa
     7261 atgaagaagc actcgggcga actgccgatc atcggcgtca acacgtactt gaacccgaac
     7321 ccgccttccg aagaggacat cgacaatatg gaaatcgccc gtgcgacgaa ggaagagaag
     7381 gagacgcaaa tcaccaactt gcgcgccttc caatcgcagc atagcgacga atccgctgaa
     7441 gcgttacgca agctgcagca agtcgctgtg accggcggca acatcttcgc cgagctgatg
     7501 gaaaccgtca aagtcgcaag cctcggccaa attacgaacg cgttgtatga agtcggcggc
     7561 caataccgca gaaatatgtg aaaatgggga acgctcttaa taagagcgtt cttttttgaa
     7621 tgtttgcccg ccattaaaaa cattttgaaa aatcgtcttc tacagtggat tttgcctgtt
     7681 ctttccacgt ataataaaga caataatggg aaagaggtga ggacatgaaa ctatatcacc
     7741 atgtattgat tatcatagtc atcatcgtcc tcatcttttc cctatacggt gtaggttatg
     7801 gcgcggttcc cttcctgctc gccgcccttt acctcggctg tctaagcgtc aaagaattcc
     7861 aacgcttcaa gaagagtaga agatggtagc ataacaggca cttcattgta tataatgata
     7921 agtatgcttt tgctcagaga aaggacgtgc gtacgttgaa tttacacgaa ttgacgaaag
     7981 aagaactcct ggaagaatcg atgatcgata ttgcatacgc aatattgacg gaacgccggg
     8041 aagcattgac acttgaacaa ttaatgaatg aaatccgtga attgacagga atgtcggaga
     8101 gccaaatgaa ggacaggctc ctccaattct acacagatat gaatatcgat ggaagattcc
     8161 ttgccattaa caacaacgaa tggggtctgc gtgaatggta tccggtcgac cagattgagg
     8221 aagaaacggc tcctgtcgtc aaagtccgca agaagaaaaa gaaaaaagcg tatgacgatg
     8281 acgaagaaga ggacgaggaa gacgaagagg aaatcgatga agaagagttg ttcgacgaag
     8341 aattcgacga gctcgatgat gacgaagacg acttggacga ggaagaagaa gaggaagagg
     8401 aaatcatcga gatcgaggaa gatctgatcg acgatgaaga aggcctcgaa atcgttcctg
     8461 acgatgaatt ggatattgac gatgaagacg aagaagatga ggaagaagag gaagaagagg
     8521 accttcttta aaactttcca cttgacttcg ggcgtgtaag gatttaatct tctatttggg
     8581 ctccttgaaa aaggacacat gaatccgtgt ataagcgctc ctcttgtaga tagactacag
     8641 tggagcgctt tttgatgttt tatacgaccg aacccctatc acaaaggagg agctacagat
     8701 gacaaaatat atctttgtaa ccggcggagt tgtctcgtct ttaggaaaag gaatcaatgc
     8761 cgcatccctc ggaagactgt tgaaagcacg aggcctgcaa gtgacgaacc aaaaattcga
     8821 cccgtacatc aatttggacc ctcggatgat gagcccatac cagcacgggg aagtgttcgt
     8881 cacggaagac ggcgctgaaa ccgatctcga catcggtcac tatgagcggt tcatcgacat
     8941 caaattgaac aaatactcca acgtcacaat ggggaaagta tactcctccg tcctgcggaa
     9001 agagcgccgc ggcgattata aaggggcgac cgtacaagtc atcccccata ttacgaatga
     9061 aatcaaaagc ctcgtcaaac gggcggggca ggaaacgaat gcggacgtcg tcattactga
     9121 aatcggcgga agcgtcggcg atatcgaatc gcttccatat cttgaagcga tccgtcaaat
     9181 gaagacggac cttggcaaag atgacgtcat gtacatccac aacacgctta tcccgtattt
     9241 gcacgctgcc ggtgaaatga aaacgaaacc gacgcagcat agcgtaaaag aattgcgcgg
     9301 actcggcatc cagccgaata tgatcgtcgt acgaagcgaa tatccggtcc ctcaggaaat
     9361 gaaggacaag attgccctct tctgcaacat caagccggaa gaagtgatcg aagcgcttga
     9421 cgcggagacg ctatacgaag tgccgatccg tctgcatgcg caaaatatgg acaagatcgt
     9481 cgtcgacttc ctcggcctcg aaacgaaaga gccggacttg acggaaatcg aagagcttgt
     9541 caaccgcgtc agaaatcttt cacgcaaagt gaaaatcgcc ctcgtcggaa aatacgtcga
     9601 actgcaagac gcgtatattt cagcggtgga agcattccgc catgcgggat acgaattcga
     9661 cacggacatc gaaatcgact ggatcaattc tgaagaagtg acgaaggaaa atgtggcgga
     9721 gaccttggaa gaggcagacg gcattttcgt tccgggcggc ttcggcgacc ggggcatcga
     9781 cggcaaaatg gaagcgatca gctacgcacg tgaaaatggc atcccattct tcggcgtcgg
     9841 ccttggcatg cagcttgcag cagtggaatt cgcccgtgac gtccttggaa taaaagacgc
     9901 tcattcagtt gaattcgaca gcgaaacgga aaatgcaatc attgaaaaca accgtgattt
     9961 cgaagaccgc tccgacatcg agcaggcgag cggggatatg cgactcggtg cgcatccgtg
    10021 caagctgaag gaaggcacga aagcgcaaga agcgtataac gacgaattgg tatacgaacg
    10081 ccatcgccac cgcttcgaat tcaacaacat ctatcgcgaa caattcgaaa acgccggcat
    10141 gatcgtggca ggcgaaagcc cggatggcca tctcgtggaa attatcgagt tgaaggacca
    10201 cccgttcttc atcggaactc aattccaccc agagttcgcc tcacgtccaa cgcgcccgac
    10261 accgctcatc cgcgaattca tccgcgcagc actcgcgcat caaggttgat agcaaatgaa
    10321 aaaacagaaa ccatcttaat tgaatggttt ctgtttttat tttggattga ggaaaggggg
    10381 tcgctcataa atttagattt ttgctcataa acttcgtccg agcgctcata aattccagca
    10441 agcgctcatg aaattaattt ttcgctcata tatcgtggca agcgctcata aaatcggatt
    10501 tgcgctcata aacttcgccg aacgctcata aattcgtttt cgcgctcata aactccgtcc
    10561 gcgcgctcat gaattcaatt tcgcgctcat aaatcccgcc aaacgctcat aaacacagct
    10621 ccacactcaa tcctcttcgc tcaccatttc gtcatacgcc atcttgaccg cctcatacaa
    10681 aaagagcgcg gccattaaat gcggctggac aatccggctg ccaagttcct cgcccggcaa
    10741 aatccccttc gtg
//
LOCUS       DASULI010000051         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_656305, whole
            genome shotgun sequence.
ACCESSION   DASULI010000051 DASULI010000000
VERSION     DASULI010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656305"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VFX34_01130"
     CDS             complement(<1..1217)
                     /locus_tag="VFX34_01130"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX5563534.1"
                     /translation="MQKTVVWFRKDFRLHDNPALSEAAKDGFVIPVFIWSEEDKREYT
                     ESEASSWWLYHSVATLEKELQAHGLKLFIKHGDEFDALVEVMEEAEADAVYFNERYEP
                     AERSKESRLTAQFESIGKKVQAFNGHLLFSPDLVNKQGEPYKVFTSFWKRCMHEVVAR
                     PLPFPVEMKGIVQGIQSLTLDQLQIIDKNKWHQKFHTYWKPGEQAAVEKWQQYLEDGI
                     SFYHAKRDFISSGSSSELSPYLAAGNISVKAVWHTAKALSEETPEAAVHQSIHTFLKQ
                     LIWRDFSYHQLIHNPDILRVPLRKQFLDFPWQGTDEQFMKWKRGQTGYPLVDAGMREL
                     WETGMMHNRVRMVTASFLVKHLLIPWQEGYNWFKHTLLDFDAANNAAGWQWVTGCGID
                     AAPYFRIFNPFLQ"
     gene            complement(1192..1917)
                     /locus_tag="VFX34_01135"
     CDS             complement(1192..1917)
                     /locus_tag="VFX34_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563535.1"
                     /translation="MTVLKLKHVTKTFGSGHKKVDALKKTEFQASRGELIAIIGPSGS
                     GKSTFLSIVGGLLTPTSGDVILNGQNVTAMTEKERAQIRLNEIGFILQASNLIPFLTV
                     AKQMKLLNKVKKGNMNVDEMERLYKDLGIGDLRGKYPSDLSGGERQRVAIAKALYSNP
                     SIILADEPTASLDSDRAYEVMELLKRETKNKKTTSIVVTHDTRLVSYCDKVYTMTDGV
                     LVLQEELTPVKKDENDAKNSCLV"
     gene            complement(1921..>2341)
                     /locus_tag="VFX34_01140"
     CDS             complement(1921..>2341)
                     /locus_tag="VFX34_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5563536.1"
                     /translation="IEIEEFIQNLPGYTEQNLTLTFMIYFLFAISAVIVAIFLYVLTV
                     QKISMFGVMKAQGISDYYLSKSVIAQTFLLAAIGVMIGLALTLTTGAFLPAAVPVSFD
                     LPTMLIYGAVLVVVAIAGAVFSVWTIVRVDPLKAIGG"
BASE COUNT          587 a          595 c          487 g          672 t
ORIGIN      
        1 ctttgcagga acggattgaa gattctaaag taaggggcag cgtcaatgcc gcatcccgtt
       61 acccattgcc agcccgctgc gttgtttgca gcatcgaagt caaggagcgt atgcttgaac
      121 cagttatagc cttcttgcca cggaattagt agatgtttga ctaaaaacga ggcagttacc
      181 attcgaactc ggttatgcat catccccgtc tcccatagtt ctcgcatgcc tgcatcgacc
      241 aatggatagc ctgtctgccc ccgtttccat ttcataaatt gctcatctgt tccttgccaa
      301 gggaaatcca gaaattgctt tctcaatggg actcggagga tgtctggatt gtggatgagc
      361 tggtggtagg aaaaatcgcg ccatattaat tgtttaagaa acgtgtggat ggattgatgg
      421 accgcagctt ccggtgtttc ctcacttaaa gcttttgcgg tatgccacac cgccttcacg
      481 ctaatgttac ctgcagcaag atacggcgac agttcggagg aggagccact tgaaataaaa
      541 tccctcttcg catgataaaa cgatattccg tcctccaagt attgttgcca tttttcaact
      601 gctgcttgct cccctggttt ccaatacgtg tgaaacttct gatgccactt gttcttgtcg
      661 atgatctgca attgatccaa cgtaagtgat tgaattcctt gtacgatccc tttcatctcc
      721 actggaaagg gcagcgggcg ggcaacgact tcatgcatgc accttttcca aaaagaagtg
      781 aacactttat acggctcgcc ttgtttattc acaagatcag ggctgaataa aaggtgtccg
      841 ttaaatgctt gcaccttttt tccaatcgat tcaaactggg ctgtaagcct actttcctta
      901 ctccgttcag caggctcata tcgttcattg aaataaacgg catccgcttc cgcttcttcc
      961 atcacctcaa caagcgcatc aaactcatct ccatgtttaa taaaaagctt caagccatgt
     1021 gcctgaagct ctttttcaag tgttgcgact gaatggtaga gccaccatga ggacgcttca
     1081 ctttccgtat attcccgctt atcttcttct gaccagataa agacgggaat gacgaatccg
     1141 tctttcgccg cctcggataa tgcagggtta tcatgtagtc gaaaatcttt tctaaaccag
     1201 acaactgttt tttgcatcat tttcatcctt cttgacagga gtcagttctt cttgcagtac
     1261 gagaacgccg tctgtcatcg tgtacacttt gtcacaatag ctgacgagac gtgtatcatg
     1321 cgttacgacg atgctcgtcg ttttcttatt tttcgtttcc ctctttaata actccattac
     1381 ttcgtacgcc cgatcagagt ctaaagatgc tgtcggttcg tcagctaaga tgatagatgg
     1441 gttgctatat aacgccttcg caatggcgac acgttgccgc tcgcctcctg acaagtcgga
     1501 tgggtacttg cctcgcaagt cgccaatgcc taagtcttta taaagccgtt ccatctcgtc
     1561 aacattcata ttgccctttt tcactttgtt taatagcttc atttgctttg ccactgtcaa
     1621 gaaaggtatt aagttcgaag cttgaaggat gaacccgatt tcattcaacc gaatttgtgc
     1681 acgctctttt tcagtcatcg ccgtgacatt ctgcccattc aagatgacat cccccgaagt
     1741 cggagtaagc aatcctccga caatgcttag aaacgtactt ttcccggaac ccgaaggtcc
     1801 gataatcgca attaattccc cacggcttgc ctggaattcc gttttcttca gcgcatccac
     1861 ctttttatgg ccgctgccaa atgttttcgt tacatgtttc agcttcagta ctgtcatcat
     1921 tcaccctcca atcgctttca atggatctac ccggacaatc gtccatactg aaaaaacggc
     1981 tcccgcaata gctacgacga cgagtactgc cccgtaaatg agcatcgtcg gcaagtcaaa
     2041 ggagactgga acagcggctg gcaggaatgc ccccgtcgtc aacgttaacg ccaatccgat
     2101 catgacgccg atggccgcta agagaaaggt ctgcgcaatg accgatttcg ataaataata
     2161 atcggagatc ccttgcgctt tcatcacacc gaacatactg attttctgga cagtcaacac
     2221 gtaaaggaaa atggctacga tgactgccga tatggcaaat aggaaataga tcatgaacgt
     2281 caacgtcaaa ttctgttcgg tgtagccagg caagttttga atgaattctt ctatttcaat
     2341 g
//
LOCUS       DASULI010000052         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_980028, whole
            genome shotgun sequence.
ACCESSION   DASULI010000052 DASULI010000000
VERSION     DASULI010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980028"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VFX34_01145"
     CDS             complement(<1..750)
                     /locus_tag="VFX34_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5563537.1"
                     /translation="MNEDQYEGPVPRRRRRRKLRIGRLFFTILFIGFIVAGLYSYFQY
                     KEGKALAKGNVIDPGPFQGDVVDPQNPSVENYLLLGIDDDGSGKSRTDTMMVLSWNKQ
                     EGSIRLVSFMRDIYADIPGYKSYKLNTAYYLNGVQTVKDTITGMFGIPIHHYAIVDFD
                     NFESIIDIAFPEGIKIDVQKEMSEKIGVKLLPGEQRLNGKELLGYARFRADAEGDFGR
                     VARQQEVISAVKEEAMRPGMLLHVPKLSGALS"
     gene            927..1025
                     /locus_tag="VFX34_01150"
     CDS             927..1025
                     /locus_tag="VFX34_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016885902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ family sporulation protein"
                     /protein_id="HEX5563538.1"
                     /translation="MGREYGGGYGSTFVLIVVLFILLIIVGASFVY"
     gene            1159..1689
                     /gene="msrA"
                     /locus_tag="VFX34_01155"
     CDS             1159..1689
                     /gene="msrA"
                     /locus_tag="VFX34_01155"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5563539.1"
                     /translation="MSKQNFEKAAFAGGCFWCMVKPFKEWEGIEDIVSGYMGGHVVNP
                     TYEDVKKGDSGHLEVVEITFNPDLFTYEKLLELYWQQIDPTDAGGQFHDRGHSYTTAI
                     FYYNDDQKKAAEQSKEELANSGKFSKPIVTDIRPAETFYRAEEYHQDYYEKEQAHYEE
                     DRAKSGRDEFIKEHWQ"
     gene            complement(1744..>2210)
                     /locus_tag="VFX34_01160"
     CDS             complement(1744..>2210)
                     /locus_tag="VFX34_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5563540.1"
                     /translation="SLVQASALLSLMMTLQIVLNLIFPIIYERIPKRRFWLLLFLTMG
                     LVGFGLFWTGYSALMWASSIFMAIPLAGLFPITLLFPLDATETAEDTNEWTAMMQTGG
                     YLIAGFLPLLIALIYDWTGNHHYTLMIFMALFVTMIFLTFLVGDREQKVKRA"
BASE COUNT          640 a          503 c          478 g          589 t
ORIGIN      
        1 cgataaggca ccggacaatt tcggtacatg aagaagcatg ccaggtctca tggcctcctc
       61 tttcacagca gaaatgactt cctgttgtct tgccaccctg ccgaaatccc cttccgcatc
      121 ggcacggaac cgtgcatagc ctaacaattc ttttccattc aagcgctgtt cgcctggcaa
      181 gagcttgacg ccgatttttt cggacatctc tttttggaca tcgattttta tcccttcagg
      241 aaacgcaata tcgatgatcg actcgaaatt atcgaagtcc acaattgcat aatgatgaat
      301 cgggatgcca aacatgccgg tgatcgtgtc tttaacagtc tgaactccat ttaaataata
      361 tgcagtattc agtttatacg atttatatcc gggaatatcc gcgtaaatat cccgcatgaa
      421 cgaaacaagg cggatggatc cctcctgctt attccaggaa aggaccatca ttgtgtcagt
      481 ccgcgacttt ccgctgccgt cgtcgtcgat gcccaatagg agataatttt cgactgacgg
      541 attttgggga tcgacgacat ctccctggaa tgggcctggg tcgatgacat tccctttcgc
      601 caatgccttc ccttctttat attgaaagta tgaatagagg cccgccacaa tgaatccgat
      661 gaaaaggatc gtaaagaaca acctgccgat ccgtaatttt cttctccttc ttcttcttgg
      721 caccgggcct tcgtactgat cttcgttcaa attcgattcc tccaaattag taagtctatt
      781 ataggacgat tggtcaatat cattggttgc gtaagaatac caatagtata tcatgtcttg
      841 actctgaaat aatgtccccg gttgaggtca attccccacg cagtcattga tactatgcat
      901 aatctatatt gaaggtggtg gtataaatgg gacgtgaata cggaggcggt tacggttcta
      961 cctttgtttt aatcgttgtg cttttcatcc tgttaattat tgtcggtgca agctttgtct
     1021 actaatcgat aaaacaataa gcacaaccac tcattggcac ttcgaggaaa ggttgcacta
     1081 cggtcattgg atggcctgta ggcagccttt ctatcgtgtg ctacaattta aataatgaat
     1141 tgagaggggt tgttagtcat gagtaagcaa aattttgaga aagcagcatt cgccggcggg
     1201 tgcttttggt gcatggtaaa gccgttcaaa gaatgggaag gaatcgagga catcgtgtcc
     1261 ggatatatgg gaggacatgt tgtcaatcct acttatgaag acgtaaaaaa aggggattcc
     1321 ggacatttgg aagtcgtgga aatcacattc aatcctgacc tctttacata tgaaaaactc
     1381 cttgaactgt attggcagca gatcgatcca acagatgcgg gcgggcaatt ccatgaccgc
     1441 ggccattcgt atacgacggc catcttttat tacaatgacg accaaaagaa agctgcagag
     1501 caatcgaaag aggaacttgc caatagcgga aagttttcaa aaccgatcgt gacggatatt
     1561 cgtccagcgg aaacatttta tcgggcagaa gaataccatc aggattatta tgaaaaagaa
     1621 caagcccatt atgaagagga ccgagccaaa tccggacggg acgaattcat aaaagaacat
     1681 tggcaataag tttgtaaatg caaacaggca aaggaaaatc aaaccgattc ctttgcctgt
     1741 ttttcatgct ctcttcactt tttgctcacg gtcccccacc aggaacgtca gaaaaatcat
     1801 ggtaacgaac aatgccatga aaatcatcaa tgtataatga tgattcccgg tccaatcgta
     1861 gatcaatgca atcaataacg gcaaaaaccc tgcgattagg tagccgcctg tctgcatcat
     1921 tgcggtccac tcatttgtat cctctgctgt ttccgtcgca tccaatggga atagcaacgt
     1981 gatcggaaat aggcctgcaa gcggtatcgc catgaaaatt gaactcgccc acatcagtgc
     2041 actgtatccg gtccaaaata aaccgaatcc aaccaatccc atcgttaaaa acaaaagcag
     2101 ccagaacctt cttttcggaa tacgttcata aatgatcggg aagatgagat tcaatacgat
     2161 ttgcagcgtc atcatcagac ttaagagcgc acttgcttga acgagggaca
//
LOCUS       DASULI010000053         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1057731,
            whole genome shotgun sequence.
ACCESSION   DASULI010000053 DASULI010000000
VERSION     DASULI010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057731"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFX34_01165"
     CDS             complement(<1..936)
                     /locus_tag="VFX34_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5563541.1"
                     /translation="MNRTSNTHLAELLMKLYGNSDEAIFFFDREGKVLAMNSAAEEII
                     EKDVYERMMAGSTKAICMVCKGYTSPEEPLTCTSCYMANPDEDISSFQVYFDTKGRGV
                     IPYSGSIQTIDEETGTRVFMLRDLTQQFRTQEELNQKLMMKRVIKAQEDERKRLSREL
                     HDSVAQEMLSSLVDLRVLKYMNIEEEVLKKVQQTEGSLMRLLDDIRHLSVELRPATLD
                     DLGLEAAFRTHFKWVEKNYGLVIHFHPELESKRYEGEIETVVYRICQEAVFNALKYAE
                     VDEMVVRLHETDGMLHLLVMDEGNGFDLKNTDPKGT"
     gene            complement(920..1396)
                     /locus_tag="VFX34_01170"
     CDS             complement(920..1396)
                     /locus_tag="VFX34_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5563542.1"
                     /translation="MKERVGFNYQQEIERLKELFGFDFIGIALVQSAERRFELRWTYV
                     TGNRSERYRRIKLQSGKGIAGLVFKTGKPMFIRNSDEEWSTGDLFNYPIVTAEGLKSF
                     GAIPLYKYDRVKGVMLVGFRSAGKLTSEQFERVKQEIGTEFGPFYNKEMVKDESDQ"
     gene            complement(1422..2114)
                     /gene="narI"
                     /locus_tag="VFX34_01175"
     CDS             complement(1422..2114)
                     /gene="narI"
                     /locus_tag="VFX34_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX5563543.1"
                     /translation="MSNQFLWVIFPYICMAVFIVGHIFRYRHDQFGWTAKSSEFIEKR
                     QLMIGSLLFHIGIIPVILGHVAGLGIPKEWTRALGVSDHMYHMGAIWGGGFFGVVTLA
                     GMLILTSRRITHRNVRKLSSTSDMIVNLLLLFIVFIGVYASLVTNNLNPGFDYRDSIS
                     VWFRSLLIFRPEAGYMAAAPLTFKLHILAGFLIFAMWPFTRLVHVWSVPLNYVGRSYI
                     LYRRSPVKAGKR"
     gene            complement(2107..2676)
                     /gene="narJ"
                     /locus_tag="VFX34_01180"
     CDS             complement(2107..2676)
                     /gene="narJ"
                     /locus_tag="VFX34_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019240765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX5563544.1"
                     /translation="MINLDRLYEEKSVFGFFANQLSYPEKITFHPNVLEESIDSSDPS
                     YPEVKTYWDLMHEHSLEEIQELYINTFDFQKDCTLFMTYVKYEDSKERGQMLARLKVL
                     YEMFGLVMPDNELSDFLPLMCEFIYAAEWRGDPRAQQSFSMLLAVLEDGSYHLMKALE
                     KYESPYFHLIKGMREVFKSCIQQEVPANE"
     gene            complement(2669..>3350)
                     /gene="narH"
                     /locus_tag="VFX34_01185"
     CDS             complement(2669..>3350)
                     /gene="narH"
                     /locus_tag="VFX34_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324613.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX5563545.1"
                     /translation="VIEQALQDGISEDWINAAKNSPVYKLAIEYKLAFPLHPEYRTLP
                     MVWYVPPLSPIMNYFEGKDSIKNPDMIFPAIEEMRIPIQYLANMLTAGDTKTVKEGLQ
                     RMAMMRSYMRALSSGKEFDESRLERVGLTAEQTKQMYRLLAIAKYEDRFVIPTSHKEG
                     TMNVYRSQGSAGYNEQMGTYGESMTQSQFSYSMVGGTDCDGCGPVSPGKTGKEIYEEN
                     FYGGIWRD"
BASE COUNT          790 a          898 c          770 g          892 t
ORIGIN      
        1 cgtgcctttc gggtctgtat ttttcaagtc gaacccatta ccttcatcca tgaccaataa
       61 atgaagcatg ccatccgttt catgcaagcg gacgaccatt tcatcaactt ccgcgtattt
      121 caatgcgttg aagacggctt cctggcaaat ccggtagacg accgtctcga tttcgccttc
      181 gtaccgtttg gattcaagtt cgggatggaa atgaatgacg aggccatagt tcttttccac
      241 ccatttgaag tgcgttcgga aagccgcctc cagcccaagg tcatcgagcg tggcgggccg
      301 cagttcgacg gatagatggc ggatgtcatc caaaagccgc atgagcgagc cttccgtctg
      361 ctgtactttc ttcaagactt cctcttcgat gttcatatat ttcaagaccc gcaaatcgac
      421 caatgaactg agcatttcct gcgccacgct gtcatggagc tcgcgggaaa ggcgcttccg
      481 ctcatcttcc tgggccttga tcacccgttt catcatcagt ttctgattca attcctcttg
      541 cgtcctgaat tgttgtgtca gatccctcag catgaatacc cgggttcccg tctcttcatc
      601 aattgtttgg atactgccgg aataagggat gactcccctg cctttcgtat cgaaatacac
      661 ttggaaggag ctgatgtcct cgtccggatt ggccatatag caggaggtgc atgtcagcgg
      721 ttcctccggg ctcgtatagc ctttgcacac catgcagatc gctttggtgc tgccagccat
      781 catccgctcg tagacatcct tttcaatgat ttcttccgct gcggaattca tcgcgagcac
      841 tttcccctcc cggtcaaaaa agaagatggc ttcatcggaa ttgccgtata gtttcattaa
      901 caattccgct agatgcgtat tactggtccg attcatcctt caccatctcc ttattataaa
      961 atggaccgaa ctccgtaccg atttcctgct tgacccgctc gaactgttca ctggtcagct
     1021 ttccggccga ccggaacccg acaagcatga cgccttttac gcgatcgtat ttatagaggg
     1081 gaatcgctcc gaagcttttg agcccctccg ccgtgacgat cgggtaattg aacaggtcgc
     1141 ctgtgctcca ctcttcatcc gaattcctga taaacatcgg ctttcccgtc ttgaaaacga
     1201 ggccggcgat ccctttgccc gactgcaatt tgatgcgccg atagcgctca ctccggtttc
     1261 ccgtcacata tgtccatctc aattcaaatc tccgttctgc cgattggacg agcgcgatgc
     1321 cgataaagtc aaatccgaat aattctttta aacgttcgat ctcttgctgg taattaaatc
     1381 ccacccgttc tttcatcacc attgctcccc acggaaaacc tttatctttt gcccgcttta
     1441 acaggcgatc ttctatacag aatatagctc ctgccgacat aattcaatgg gacgctccat
     1501 acatggacaa gccttgtgaa tggccacatt gcgaaaatga ggaagcccgc gagaatatga
     1561 agcttgaacg tcaatggggc tgccgccata taacctgctt ccggcctaaa gatgagcaag
     1621 gaccggaacc agacggagat ggaatcacga tagtcgaatc ccgggttcaa gttatttgtc
     1681 actaatgatg catacacacc gatgaaaacg atgaatagaa gcaacaagtt gacgatcata
     1741 tcagacgtcg aagaaagctt tcggacattg cggtgagtaa tgcgccgcga agtaaggatc
     1801 agcattccgg caagtgtgac gacaccgaag aaccctcctc cccaaatggc tcccatatga
     1861 tacatatggt cactgacgcc gagtgcacgc gtccactcct tcggtatgcc gagccccgcc
     1921 acatggccaa gaatgacggg gatgatgccg atatggaaaa gcaggctgcc gatcatcagc
     1981 tgtctttttt caatgaactc acttgatttc gccgtccagc cgaattggtc gtgccgatag
     2041 cggaaaatat gcccgacgat aaatactgcc atgcaaatat aagggaaaat cacccataag
     2101 aattggttac tcattggcag gaacctcctg ttggatacaa gacttgaata cctccctcat
     2161 ccctttgatg aggtggaagt aggggctctc gtatttttcc agagccttca tcaaatgata
     2221 ggatccatcc tccaacacag cgagcagcat cgaaaagctt tgttgggcgc gcggatcccc
     2281 tctccattcc gcggcgtaga tgaattcgca cataagcgga aggaagtctg atagttcatt
     2341 atccggcatg acaagcccga acatttcata gagcactttc aaccgggcaa gcatttgccc
     2401 gcgttccttt gaatcttcgt atttcacata cgtcatgaac agcgtgcagt ctttttggaa
     2461 atcgaatgtg ttgatgtaca actcttggat ttcttcaaga ctatgctcat gcatcaggtc
     2521 ccaatacgtc ttcacttcgg gataggacgg atcggaagag tcgatcgact cttccaatac
     2581 gttcgggtga aacgtgatct tttcgggata ggacagctgg ttggcgaaaa atccgaagac
     2641 gcttttctct tcgtatagtc ggtccagatt aatcacgcca gatcccccca tagaagtttt
     2701 cttcgtatat ctccttgccc gttttccctg gggagaccgg tccgcatcca tcacagtcgg
     2761 tgccgcctac catcgaatag ctgaattggc tttgcgtcat gctttcgcca tacgtgccca
     2821 tttgttcgtt gtaaccggcc gagccttgtg aacgatagac gttcatcgtg ccttccttat
     2881 gggaagtcgg gatgacgaag cggtcttcgt atttggcgat agcgagcaaa cggtacattt
     2941 gcttcgtctg ttcggctgtt aggccaacac gttcaagtcg ggattcgtca aattccttgc
     3001 cggacgatag tgcgcgcata taggagcgca tcatcgccat ccgctggagt ccttctttca
     3061 cggttttcgt atcacctgcc gtcagcatat ttgctaagta ttgaatcgga atcctcattt
     3121 cctcgattgc cgggaagatc atgtcaggat tcttgatgga atccttgcct tcaaagtagt
     3181 tcatgatcgg gctgagtggc ggcacatacc agaccatcgg caatgtgcgg tattccggat
     3241 gcagcgggaa tgcaagcttg tattcgatcg ccaatttata gaccggcgag tttttcgctg
     3301 cattgatcca atcctccgaa atgccgtcct gcagcgcctg ttcaattact
//
LOCUS       DASULI010000054         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_901920, whole
            genome shotgun sequence.
ACCESSION   DASULI010000054 DASULI010000000
VERSION     DASULI010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901920"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFX34_01190"
     CDS             complement(<1..426)
                     /locus_tag="VFX34_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide excision repair protein"
                     /protein_id="HEX5563546.1"
                     /translation="MLCDNCKERPASVIFTQEGMGGSTERHLCEKCAFQTQMFHFDPN
                     QEPLTIQQFLSHWFGGSELLQPFQQSRENTTEGPTCPDCQLTFRKFLDLGKFGCPSCY
                     DSFREYLPRVFSKLHNGHTKHVGKIPVSFNERFALRKKVE"
     gene            complement(454..924)
                     /locus_tag="VFX34_01195"
     CDS             complement(454..924)
                     /locus_tag="VFX34_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEX5563547.1"
                     /translation="MRNISDIIEGYLKAIIEKEDSGTVEIKRSEVAEKFQCVPSQINY
                     VINTRFTVERGYAVESKRGGGGYIRIYRVQTNSHKELIEHILEGIQQGATYTMAEGII
                     YRLLKEEVVSEREARIILAAVDRTTLHYPLPERDMIRARILQAMLQTLIYKQLE"
     gene            complement(1674..1928)
                     /locus_tag="VFX34_01200"
                     /pseudo
     CDS             complement(1674..1928)
                     /locus_tag="VFX34_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2093..>2520
                     /locus_tag="VFX34_01205"
     CDS             2093..>2520
                     /locus_tag="VFX34_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563548.1"
                     /translation="MQKNFFQSEKPPSKMSKANWFYYYFVLTLIGLLGVGALYLFFIM
                     LPEGVWLLNLLIGGIALFLGWVFVKILLLPRQKGPTTFKKMYDAAGYTTYFINEWTGE
                     TESVSFRYDQITDLLIGLWTNPGFKGRKNDYVGARLLYRYR"
BASE COUNT          616 a          579 c          578 g          747 t
ORIGIN      
        1 ctcgactttc tttctcaaag cgaaccgttc attaaaggaa acgggtattt tcccgacatg
       61 tttcgtatgc ccgttatgga gcttgctgaa gacgcgcggc aaatactccc tgaacgagtc
      121 gtagcatgat ggacaaccga atttgccgag gtcgaggaat ttacggaatg tcaattggca
      181 atcagggcag gtcgggcctt ccgttgtgtt ttcccttgat tgctgaaaag gctgcaacaa
      241 ttcactgccg ccgaaccaat gggaaaggaa ttgttggatt gtcaacggtt cctgattggg
      301 atcgaaatga aacatttgtg tttggaacgc gcatttctca catagatggc gttccgtgga
      361 cccgcccatg ccttcctggg tgaagatcac ggaagccggc cgttctttgc aattgtcgca
      421 tagcattgtg tgccacctcg tattcgtcga attctattca agctgcttgt aaataagcgt
      481 ctggagcatc gcctgcaaaa tccgcgcccg gatcatgtca cgttccggca atgggtaatg
      541 cagggtcgtc cggtccaccg ctgcgagaat gatcctcgcc tccctttccg aaacgacttc
      601 ttcttttaac agcctgtaga tgattccctc agccatcgta taggtcgcgc cttgttggat
      661 tccttctaaa atatgttcaa tcaattcttt atgcgaattt gtctggacgc ggtaaatacg
      721 gatgtatcct ccgcctcctc gtttcgattc gacggcatag ccgcgttcga cggtgaagcg
      781 tgtgttgatg acatagttga tttgcgaagg gacgcattga aatttctccg cgacttcgct
      841 ccgtttgatt tccacggtcc cgctatcttc tttttcgata attgccttta aatacccttc
      901 aataatgtcg gaaatgtttc gcatgatgtc acctcttttc ttcgggtgct aagccattga
      961 ctttgactat ctttgactta attgtacatg aaaatacatc tagcgtgcaa tttatatgca
     1021 gattaacctt ctttgaaaag gggcagttgc tttccgtttc agcacttcgc tttccgcggg
     1081 cgtcgcctca gccaatcgaa cagcgaaggg ttcgatttgc cgtatttctg cgttttgtgc
     1141 agaaattaag gcatcctgct cccaacgcca cgcttttggt cgcaaaagcc gttcttcgta
     1201 acggctttcg caggagtctt cgtgctttca cttcatgcaa ctgctgataa gtcatttgaa
     1261 aactgggtct caattataaa ttccatttgt aatttccgac cagaagatat tttacttata
     1321 attaacagtg tagggtaata aggtagttga ggattttgtc tgacctcttc ttcctcttca
     1381 cggaatttcg tttgaaaggg acagatggaa gcgattgagg caaacagcct tctttctcac
     1441 cccgagcagt ctgtcctttt gaataaactc ttataataaa atttcttgga aatgatgtac
     1501 ttccaaaaac gcataaaatg ctggaaaggc tcatataatt gttggaaaag cttataagtc
     1561 gctagaaaag tccataaaac gaaggagacg cgcataaatc actaaattca ttcataaacg
     1621 cccgcaaaga tgcataaaag gcagcctccg aaattccggg ggctgccttt tattcattca
     1681 atcgtagcgt tcttgagttc tttgactggg aggaagagtt ctcctgtttc gtaataggtg
     1741 agagcgaggt ggtcgccttc ttggatgtag attgctagcg ggacggcttc ggttgtgacg
     1801 atgtagttcc ggccgtcgtc ggcaaggatc gaaacgatcg taaagtcgcc ggagcgttct
     1861 ttatagaccc ggacaacgtt gacggaggct tttccttcct cggcgttcga gctgccgtct
     1921 actgtgcttc attcggtgaa gtgggataca catccgaaca ctcttttcca ttaaaaataa
     1981 acctcttcta ctatacggat gaaatagcta aacgattcgt attcctggtg atgaatttga
     2041 atccttcctc cctcccctta cgtacagaat atggaaggga ggagttgaat cgatgcaaaa
     2101 gaactttttc caatccgaaa aaccgcctag taaaatgtca aaggcaaact ggttttatta
     2161 ttattttgtc ctcacactga tcggattgct tggcgtaggc gctttgtatt tatttttcat
     2221 catgcttcca gaaggggttt ggcttttgaa cttgctgatc ggcggcattg ctctgttcct
     2281 tggctgggtg ttcgtcaaaa ttcttctgct gccacgtcaa aagggaccga caacgttcaa
     2341 aaaaatgtac gatgcggccg gctatacgac ttatttcatc aacgagtgga ccggcgaaac
     2401 agaatccgtc tctttccgct atgatcaaat aaccgatcta ctcatcggtc tttggacgaa
     2461 tccagggttc aagggaagaa aaaatgatta cgtcggagcg cgtcttctct atcgttaccg
//
LOCUS       DASULI010000055         5577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_990892, whole
            genome shotgun sequence.
ACCESSION   DASULI010000055 DASULI010000000
VERSION     DASULI010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990892"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFX34_01210"
     CDS             complement(<1..586)
                     /locus_tag="VFX34_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563549.1"
                     /translation="MVKLNRFIQFNDETIDANLLLLYERLGRALADADYLELTERKLF
                     EFQPKEGIVSISVFWRHRSEKIMHAGRLSDVYLLTAGFWKHFSVSDWLRFRARYENHP
                     LQKFAEELLLMLEEFRLMDTVEKERPGTSKAFLIRRQLLLSFHRDQLLSNSNKKFIAD
                     ALLNQLFITLHEGLLTDTHTIEWNLPISQIMMIAG"
     gene            complement(597..1466)
                     /locus_tag="VFX34_01215"
     CDS             complement(597..1466)
                     /locus_tag="VFX34_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5563550.1"
                     /translation="MYMDSLIVRERSIRQAREYENQDASLIQEGGYISPIPDLWEDIL
                     IGIVLKKPVLLKGPSGSGKTKLAQSISHFFNQPMHSVNCSVDLDAESLLGFKTINAKS
                     GQSIIEFVEGPVVQAMKKGHILYIDEINMAKPETLPILHSVLDHRRMLTNPFTSEVIH
                     AHPDFTVVAAINEGYVGTSPMNEALKNRFISFSIPYLSGEQLREIIQTLFPQANSALV
                     NTMMNINEDLRKQVMNGLLAEEAASVRSLLDAIALAEHIPVRRAIQYAIAEKLEDRME
                     RDLVSELVETWVK"
     gene            complement(1525..1722)
                     /locus_tag="VFX34_01220"
     CDS             complement(1525..1722)
                     /locus_tag="VFX34_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6501 family protein"
                     /protein_id="HEX5563551.1"
                     /translation="MSFNEWTTAPTLRKVVCKHANAEKYVVSDCLTPGKEYEVKNETD
                     EFIFIVDNTGKVGGFYKTYFE"
     gene            complement(1794..3026)
                     /gene="odhB"
                     /locus_tag="VFX34_01225"
     CDS             complement(1794..3026)
                     /gene="odhB"
                     /locus_tag="VFX34_01225"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5563552.1"
                     /translation="MAEIKVPELAESITEGTIAKWLKQPGESVDKGEFIVELETDKVN
                     VEVISEEAGVIQELLAAEGDTVEVGQVIAVVGEGSGAPAAQPAAPAPKAEEAPAAPAP
                     QEAAAEQTTEQTSSDRTIASPAARKLAREKGIDLAAVSPVDPMGRVRAQDVSAHGTAP
                     KAPAAPAAPAKATAPVEDGRETRERMTRRRQTIAKRLLEVRQSTAMLTTFNEIDMTAV
                     MELRSRKKDEFFDKNDVRLGFMSFFTKAVVAALKKYPYVNSEIDGDDIILKHYYDIGI
                     AVSTEGGLVVPIVRDADRKNFAEIESTIGELAGKARDNKLTIADMSGGSFTITNGGVF
                     GSLFSTPILNGTQVGILGMHTIQKRPVAVGDKVEIRPMMYIALSYDHRVIDGKDSVGF
                     LKMVKELIENPEDLLLGS"
     gene            complement(3090..>5577)
                     /locus_tag="VFX34_01230"
     CDS             complement(3090..>5577)
                     /locus_tag="VFX34_01230"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5563553.1"
                     /translation="NYGLSENDLQSMPASLFLKNPPAQVESGLDAVNYLKSLYTGKIA
                     YEFSHVIDEEERTWIQSKIENGEISVQLSEDDKKALLERLTKVEGFEKFIHRTFVGAK
                     RFSIEGLDSLVVLIDELVRRSEADKVKKVLIGMAHRGRLNVLTHILNKPYEMMFAEFA
                     GVPSAPFLPEDGSLETTHGWFGDVKYHMGALYKAPSGTERFLAYNPSHLEVVNPVVAG
                     QTRAAQETTNHEGLPEQDMKAAYAIMIHGDAAFPGQGIVPETFNYSRVRGYKTGGSIH
                     IIANNTIGFTTEFYDSRSTHYSSDPAKGFEVPVLHVNADSPEDVIAVAAFAFEYRQKF
                     GKDILIDLIGYRRYGHNEMDEPLVTNPMMYHLIHKHPTARQLYGSQLVEANILKEEDV
                     DKLDADVFAIMQSAYDKVRENSSKAKESSNATPENVMAGYPRDLETGISQERLQKMNE
                     ELLSYPSDFTVFSKLDRILKRREEPFKGKGKVDWAHAEQLAFGSILQDGKPIRITGQD
                     VQRGTFAHRHLVLHDEKTGEEFVPLHHISDSKASFVAYNSPLTEFAVVGYEFGYNLEK
                     DQALSIWEAQYGDFANMAQVMFDQFISSSHSKWGQQSGMVILLPHAYEGQGPEHSSAR
                     LERYLQLSAENNWTVANISSAANYFHVLRRQAMMLGTEVERPLVIVSPKSLLRHPLVG
                     AEVSDLAEGHFQTVLEQPGTGQNPDKVKKILFASGKMAIDLAEKVKDGEGFDHLHIVR
                     VEQLYPFPSDKIAEIAARYPKAKELVWVQEEPKNMGSWSFALEYMQEIAGDKKSVSYV
                     GRIHRASPSEGNGEAHKAEQQRIIEEALKK"
BASE COUNT         1389 a         1352 c         1193 g         1643 t
ORIGIN      
        1 accctgcaat catcattatc tggctgatcg gcaaattcca ctcgatagta tgggtgtcgg
       61 tcaacagtcc ttcatgcaaa gtgatgaaca actggttcaa taacgcatca gcaatgaatt
      121 tcttattgga atttgacagg agttggtccc gatgaaagct gagcagaagt tgccgacgta
      181 tcaaaaatgc ttttgatgtc cctggccgtt ccttttcaac agtatccatc agcctgaatt
      241 cctccaacat gagcaacagt tcttcggcaa acttctgtaa aggatggttt tcgtaccttg
      301 ccctgaatcg gagccagtcc gaaaccgaaa aatgtttcca gaaacccgct gtcaataaat
      361 acacgtctga taatctgccg gcatgcatga ttttttcgga acgatgcctc caaaacacac
      421 taatcgaaac aatgccttct ttcggctgga attcgaacag tttacgctct gtcaattcca
      481 ggtaatccgc atctgctagc gccctgccta gacgttcgta aagcaaaagt aaattggcat
      541 cgatcgtttc atcattgaat tgaatgaacc ggtttaattt gaccattgct tccgcctcac
      601 ttcacccatg tctccactaa ttcactgact agatcccttt ccatcctgtc ttcaagcttt
      661 tctgcaatcg cgtactggat ggcacgcctt accggtatat gctctgcgag cgctatcgca
      721 tccaataaac ttctgacaga agccgcttcc tcagccaata gaccgttcat cacttgtttt
      781 ctcaaatctt cattgatgtt catcatcgta ttgaccaatg ccgaattagc ttgcggaaat
      841 agtgtttgaa tgatttcccg caattgttca ccggacaaat aagggatcga aaacgagatg
      901 aagcggtttt tcaatgcttc attcatcggt gaagtcccaa catatccttc attaattgca
      961 gcaacaactg taaaatcggg atgcgcatgg atcacttcac ttgtgaaagg atttgtcagc
     1021 atacgacgat ggtcaaggac actatgtaag atgggcagcg tttccggctt tgccatattg
     1081 atttcatcta tatacaggat gtggcctttc ttcattgctt gaacgaccgg gccttctaca
     1141 aattcgatga tggattggcc gctttttgca ttaatcgttt taaatcctag aagcgattcc
     1201 gcatccaggt cgacggaaca gttcacactg tgcatcggtt ggttgaaaaa atgtgaaatg
     1261 ctctgtgcaa gcttcgtttt accggagccg gaaggacctt tcaataagac cggctttttc
     1321 aatacaattc caattaaaat atcttcccaa agatcgggaa tcggagaaat atatccccct
     1381 tcctggatta gcgaagcatc ctgattctcg tattccctag cttgccgtat tgacctttca
     1441 cgtacaataa gagaatccat atacatcatc ctccttctat tgaaaaatgc ccgatgccat
     1501 aatacttggc atcgggcgaa aaaatcattc gaaatacgtt ttataaaagc cgccgacttt
     1561 accggtatta tccacgatga aaatgaattc gtctgtctcg ttcttcactt catattcttt
     1621 gcccggagtt aaacaatcgg aaacgacata cttttccgca ttcgcatgtt tacatacgac
     1681 tttccgcagt gtcggagcag tcgtccattc gttgaaagac atttagttca ccatccattt
     1741 gccgtaatgt gaaaaagccc gagatgctcg ggctgttttc agttatttat caatcaagaa
     1801 ccaagaagca gatcttcagg gttttcaatc agttccttaa ccattttcaa gaagccgact
     1861 gaatcctttc catcgattac acggtggtcg tatgataatg caatgtacat catcggacgg
     1921 atttccactt tatcgccgac tgcgacaggg cgtttttgga ttgtgtgcat tccaaggata
     1981 ccaacttgtg taccattcaa gatcggtgtc gagaacaatg aaccgaatac gccgccgttc
     2041 gtaattgtga atgagccgcc ggacatatct gcaattgtca atttattatc tctcgcttta
     2101 ccggcaagct cgccgattgt agactcgatt tcagcgaaat tcttacgatc tgcatcgcgt
     2161 acgatcggaa cgaccaatcc gccttcggtc gacactgcga taccgatatc gtaatagtgt
     2221 ttcaagataa tgtcatcgcc atcgatttcc gaattgacgt atggatattt tttaagagcc
     2281 gcaacgaccg cttttgtgaa gaaggacatg aatcctaggc gcacatcatt tttatcaaag
     2341 aactcatctt ttttacgtga acgcagttcc attactgcag tcatatcgat ttcattgaat
     2401 gttgtcaaca ttgcagttga ttgtctaact tccaacaagc gctttgcgat cgtttgacgg
     2461 cggcgcgtca ttctttcacg agtttcacga ccgtcttcaa caggtgctgt tgctttagct
     2521 ggagcagcag gagcagcagg cgcttttgga gctgtaccat gcgctgaaac atcttgtgca
     2581 cgaacccgcc ccattggatc tactggggaa acagctgcaa ggtcgatgcc cttttcacgt
     2641 gcaagttttc tagcagccgg actagcgatt gtacgatcag aagaagtctg ttcagttgtt
     2701 tgttcagcag ctgcttcttg aggtgccggt gcagccggag cttcttccgc tttcggtgca
     2761 ggggctgcag gttgagcagc tggtgctccg gaaccttcac ctacgactgc gatgacttga
     2821 ccgacttcaa ctgtatcacc ttcagcagca agaagttctt ggatcactcc tgcttcttcg
     2881 gagatgactt caacgtttac tttatccgtt tccagttcga cgatgaactc gcccttgtca
     2941 acagattcac ctggttgttt caaccatttt gcaattgttc cttcagtaat cgattctgct
     3001 agttcaggta ctttgatttc agccacaata aattcctcct ctgttctacc gttcaacggt
     3061 ggtaacaaac atcttttatt aatgagcaat cattttttca aagcttcttc gataatgcgt
     3121 tgttgctccg ctttgtgcgc ttcgccgttt ccttcggatg ggcttgcgcg gtgaatacgt
     3181 cctacatagg aaacggattt tttatctcct gcgatctcct gcatgtattc caaagcgaat
     3241 gaccaagatc ccatgttttt aggttcttct tggacccata caagctcttt cgctttcgga
     3301 tagcgcgctg cgatttctgc aattttatcg gacgggaatg gatatagctg ctccacgcgt
     3361 acgatatgca agtggtcaaa accttctcca tccttaactt tttcggcaag atcaatcgcc
     3421 attttaccgc tggcgaatag gatttttttc accttgtctg gattctgacc tgttccaggc
     3481 tgctcaagaa cagtttggaa gtggccttcc gccagatcgc tcacttcggc accgacaagt
     3541 ggatgacgaa gaagtgattt tggagatacg atgactagtg gacgttccac ttcggttcca
     3601 agcatcatcg cctgtctgcg caaaacgtgg aagtagttgg ctgcgcttga aatgttcgca
     3661 accgtccagt tgttctccgc actcagctgt aaatatcttt ccagacgtgc acttgagtgt
     3721 tcaggtcctt ggccttcgta tgcgtgcggc agaagaatga ccatgccgga ctgctggccc
     3781 catttcgaat ggctggaaga aatgaattgg tcgaacatca cttgtgccat atttgcaaag
     3841 tccccatatt gggcttccca aatcgacaat gcctggtctt tctccaaatt ataaccgaat
     3901 tcgtaaccga caactgcgaa ctcagtcaat gggctgttgt aagcgacaaa tgatgctttg
     3961 gagtcgctga tatgatgaag cggtacaaat tcttcccctg tcttttcgtc atgcaacacg
     4021 aggtgacgat gcgcgaaagt gcctcgttgg acatcttggc ccgtaatgcg gatcggcttg
     4081 ccgtcctgga ggatggaacc gaatgcaagt tgttcagcgt gtgcccagtc cactttccct
     4141 ttacctttga aaggttcttc acgtcgcttc aatatccgat ccaatttgct gaatacggtg
     4201 aagtcggaag gataagaaag aagttcttcg ttcatctttt gcaatctttc ctggctaata
     4261 ccagtttcaa gatccctcgg atagcccgcc atgacatttt caggcgtcgc attggaactt
     4321 tctttcgctt ttgaagaatt ctctctcact ttgtcgtatg cggattgcat gattgcaaag
     4381 acgtctgcat ccagcttatc gacatcttct tctttgagga tgtttgcctc aacaagttgt
     4441 gatccgtaaa gctgacgtgc agtcgggtgt ttatggatca aatgatacat catcgggttc
     4501 gtcactaatg gttcatccat ttcgttatgc ccgtaacggc gatagccgat caagtctatt
     4561 aaaatatcct tgccgaactt ttggcgatac tcgaatgcaa aagccgccac cgcaatgaca
     4621 tcttctgggc tatccgcgtt gacatgcaat actggaactt caaacccttt tgcaggatcc
     4681 gaagagtagt gagtcgatct tgaatcgtaa aattcagttg taaaaccgat tgtattattc
     4741 gcgataatgt ggattgaacc accggtctta tatccacgta cgcggctata gttgaacgtt
     4801 tccggaacaa ttccctgtcc agggaatgct gcatccccat ggatcataat tgcataggcc
     4861 gctttcatat cttgttctgg caaaccttca tggttagtcg tttcttgggc ggcacgggtc
     4921 tgacctgcta ctaccggatt gacgacttcc aagtgcgaag ggttgtaagc taagaagcgt
     4981 tctgttcctg aaggagcttt gtaaagggct cccatatggt atttaacatc accgaaccag
     5041 ccatgtgtcg tctcaagcga tccatcttcc ggaaggaatg gcgctgaagg gacgcctgca
     5101 aattcggcaa acatcatttc ataaggttta ttcaaaatat gggtcaatac attcaaacgg
     5161 cccctgtgtg ccatgccaat caatactttt ttcactttgt cggcctcgga acggcgcacc
     5221 aattcatcaa tgaggacgac tagcgagtca agaccttcta ttgaaaagcg ctttgccccg
     5281 acaaaagtac ggtgtatgaa tttctcaaaa ccttcgacct ttgtgagtcg ctcgagcaag
     5341 gctttcttat catcctcgga aagctggaca gagatttcac cgttttcaat tttcgattgg
     5401 atccaagtcc gttcttcctc atcaataaca tgagagaatt catatgcgat ctttcctgta
     5461 tataatgact tcaggtaatt caccgcatcc agtccgcttt ctacttgtgc gggcggattt
     5521 ttaaggaaca aggaggctgg catcgactgc aaatcgtttt cggataatcc ataattc
//
LOCUS       DASULI010000056         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_181668, whole
            genome shotgun sequence.
ACCESSION   DASULI010000056 DASULI010000000
VERSION     DASULI010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181668"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFX34_01235"
     CDS             complement(<1..718)
                     /locus_tag="VFX34_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HEX5563554.1"
                     /translation="MFEKEEGKLREMKDQLENIRLPLDEADEAISAGYHRAKREKVKM
                     RKRRKRIWSLVAAALIFFSFVTSIRVSPAFANAVAAIPGMERFVNLIQYDKGLDAILK
                     NDYYQKVDAAQTINGLTLAIDGVILDETGMNIYYTMKSKEAVKGLSIKSVDLKNEQEI
                     PPSGISYGGSIPDDKTQEWSSQIVYTFGEPTLFKDLSFTLDLDASYQGKNILFSLPFD
                     LKENVKQGKTFLLNEEVEIES"
     gene            complement(711..1229)
                     /locus_tag="VFX34_01240"
     CDS             complement(711..1229)
                     /locus_tag="VFX34_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016200837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5563555.1"
                     /translation="MDEKEISEKAIRGDDEAFLALMQLHKEALLRTAIAFLKNEADAL
                     EALQEVTYRAYKKIHTVREPAYVKTWMIRIMMNYCQDQLKRGKRYVMTAEVDETIGKE
                     DDYKLELSEALATLKPDEQQLIYLKYFQDVKIKEIAELSSIPEGTVKSRLHKILRSLR
                     SHFRDKGGMDHV"
     gene            complement(1411..2871)
                     /locus_tag="VFX34_01245"
     CDS             complement(1411..2871)
                     /locus_tag="VFX34_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX5563556.1"
                     /translation="MTESKKFGWHFDNSYTRLPEPFFAKTDPEEAPSPELVVFNHPLA
                     ESLGLDAESLEGEEGMDILAGNKIPEGAAPIAQAYAGHQFGHFTMLGDGRAVLLGEQL
                     TPEGKRVDIQLKGSGRTPFSRGGDGRAALGPMLREFIMSEAMHALGIPTSRSLAVVVT
                     GAPVYREDTLEGAVLTRIAASHIRVGTFQYAAARQVIEDLRTLADYTIDRHYPEVAND
                     PNPYVSFLQKVMEKQASLIAKWQLVGFIHGVMNTDNMAISGETIDYGPCAFMDTYDPA
                     TVFSSIDRQGRYAYGNQPGIAEWNLARFAETLLPLFDEDSEKAIEIATQTLSGFAKMY
                     HEQWLAGMRNKLGLFNSEEQDVTLVKDLLKIMKEHQADFTNTFRALTFGDLTVLPMCK
                     APEFDEWHTRWKSRLERQPESEADVKKLMRKSNPSIIPRNHRVEEALDAATEGDFSVM
                     EKLLAVLENPYAYTAEQEKYVAPASSTTPYRTFCGT"
     gene            complement(2922..3302)
                     /locus_tag="VFX34_01250"
     CDS             complement(2922..3302)
                     /locus_tag="VFX34_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5563557.1"
                     /translation="MIHKIGQIMLYVNNQDEAKAFWTEKFGFTVVSEEDNGQGMKWIE
                     MAPTGQAETSIVLHNKKLIAEMQPELNLATPSLLFFTENLDALYEDLKSKQVKVGELV
                     TMPTGRVFNFADHEDNYFAILEKK"
     gene            3468..3713
                     /locus_tag="VFX34_01255"
     CDS             3468..3713
                     /locus_tag="VFX34_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HEX5563558.1"
                     /translation="MESMKIAVEQPYEDVMNALLAKGYEAKMFNTDEDVTGYDIGVVR
                     AINEGNTHEFNFPVITMKGMSIDDVVHAVEQKANLLQ"
BASE COUNT          912 a          968 c          790 g         1151 t
ORIGIN      
        1 gtgattcaat ctcgacttct tcattcaata aaaatgtttt accctgcttt acattctctt
       61 tcagatcaaa aggcaatgag aataaaatgt ttttcccttg ataggaagca tccaagtcca
      121 aggtgaatga taagtcttta aacagagttg gttcgccaaa agtgtacaca atttggctag
      181 accattcttg ggttttatca tcagggatgg agccgccata tgaaattcca cttggcggaa
      241 tttcctgctc atttttcaag tcgactgatt taatactgag ccctttcaca gcttccttag
      301 acttcattgt gtaataaata ttcatcccgg tttcatccaa gatgacgcca tctatcgcca
      361 acgtcagccc atttatcgtc tgagccgcat cgactttttg gtaataatca tttttaagaa
      421 tggcgtccag tcctttatcg tactgaatca gattcacgaa tctttccatg cccggtatcg
      481 ctgctaccgc gtttgcaaat gccggggaca cgcggattga agtgacaaag gagaagaaga
      541 taagcgctgc cgctaccaat gaccaaatcc gttttcgcct ctttctcatt ttcacctttt
      601 cacgcttcgc tcggtgatat cctgcactga tcgcttcatc cgcttcgtcc aaaggtaggc
      661 gaatattctc cagctggtcc ttcatttccc gcagttttcc ttcttccttc tcaaacatga
      721 tccattcctc ctttgtctct gaagtggctt cgcagtgatc gcaaaatctt gtgcagcctt
      781 gatttaaccg tgccttccgg tatacttgat agttctgcaa tttcctttat ttttacatcc
      841 tgaaagtatt taagataaat taactgctgt tcgtctggtt tcaacgtggc aagtgcttcg
      901 gacaattcta atttataatc atcttccttg ccgattgttt cgtccacttc cgccgtcatg
      961 acataccgtt ttcctcgctt tagctgatct tggcaatagt tcatcataat gcggatcatc
     1021 cacgtcttca catacgccgg ttctctaacc gtatgaatct ttttatatgc acgataggtt
     1081 acttcttgaa gagcttccaa tgcatccgcc tcatttttca agaaagcaat tgctgttcga
     1141 aggagcgctt ctttatgaag ctgcatgagc gctagaaagg cctcatcatc tccacgtatc
     1201 gctttttcac tgatctcttt ctcgtccatt tcccccacct cattcgccct tcttactcat
     1261 tagactgcgt aacgttgaaa aagttcaaaa gttattcaac taatttgatt gcaagtctta
     1321 tatacggaag gaaatggttt tggttgcgtc aaaaaaagac caaatccatt aaggtttggt
     1381 ctttcgaagt ttacggaacg cttgccttca tcacgtcccg cagaatgttc tgtacggggt
     1441 tgtcgatgaa gctggcgcga cgtatttttc ttgttcagct gtatatgcat atgggttttc
     1501 aagcacagca agcagtttct ccatcacgct gaagtcccct tcggttgcgg catccaacgc
     1561 ttcttctacg cggtggttgc gcgggatgat ggagggattg cttttccgca tcagcttttt
     1621 tacatcagct tctgattccg gctgtctttc cagccgcgat ttccagcggg tgtgccattc
     1681 gtcaaactcc ggtgccttgc acatcggcag aacagtgaga tcgccaaacg tcaatgcacg
     1741 gaatgtattc gtaaaatccg cttgatgttc cttcattatt ttcaatagat ctttcacgag
     1801 cgttacgtct tgctcttcac tattgaacaa acctagcttg tttctcattc ctgcaagcca
     1861 ttgctcatga tacatcttcg cgaagcctga aagtgtctgc gtcgcaattt caatcgcttt
     1921 ttcggagtct tcatcaaaga ggggcaaaag tgtttcggca aaacgggcaa gattccattc
     1981 agcgattccg ggttgattgc catacgcata tcggccctgc ctgtcgatgg agctgaaaac
     2041 tgttgccgga tcatacgtgt ccatgaaggc gcacggtccg tagtcgatcg tttcgccgct
     2101 gatcgccata ttgtccgtgt tcatgacgcc atgaatgaat ccgaccaact gccatttggc
     2161 aattaatgat gcttgctttt ccatcacttt ttggaggaac gacacgtatg gattcgggtc
     2221 gttcgccact tcaggataat gcctgtcgat cgtataatcc gcaagcgtcc gcaaatcttc
     2281 gatgacttgg cgcgccgcag cgtattggaa cgtgccaacc cgtatatggc ttgcagcaat
     2341 ccttgtcaaa acagcccctt cgagagtgtc ctctcgatat actggcgctc ccgtcacaac
     2401 aactgcaagg cttcgcgacg tcggaatgcc aagtgcatgc atcgcttcgc tcatgatgaa
     2461 ttcacgcaac atcggaccaa gcgctgcccg tccatcgcct ccacgggaaa atggtgttct
     2521 gcccgaccct ttcagttgaa tatccacccg cttgccttcc ggtgtaagct gttcgccaag
     2581 cagcacggcc cgtccatccc cgagcatcgt aaaatggccg aattggtgtc cggcatatgc
     2641 ttgcgcaatc ggcgccgcgc cttcaggtat cttgtttcca gcgaggatat ccattccctc
     2701 ctcaccttct aacgattctg catccaaccc gagcgattcc gccaatggat gattgaatac
     2761 gaccaactct ggtgatggtg cctcttcagg gtccgtcttc gcgaagaatg gctccggcag
     2821 acgggtgtag ctgttatcga aatgccatcc gaactttttg ctctctgtca ttatcttcaa
     2881 ctcccgtttc taagatgtcc gcttcgcaat ttaaaaggga tttatttctt ttccaatatg
     2941 gcaaaataat tatcttcatg gtcggcaaag ttgaacactc ggcctgtagg cattgtaacc
     3001 agttcgccga ctttcacttg tttgctttta aggtcttcat aaagtgcatc cagattttca
     3061 gtgaaaaata ggagcgaagg cgttgcaaga ttcaattccg gctgcatttc tgcgatgagt
     3121 tttttattat gcagtacgat gctcgtttcg gcttgtcctg ttggtgccat ttcaatccac
     3181 ttcatccctt ggccattgtc ttcttctgat accacggtga agccgaattt ctccgtccaa
     3241 aacgcttttg cctcatcctg attgtttaca tacaacataa tttgcccgat tttatgaatc
     3301 attttcaata ccccgctttc catatttttc atcctgttaa ggttaagtgc ttatccctat
     3361 cttataacat atactcggtc aaccgcacgt aacaacacat tactcataaa ttcatgaata
     3421 cggggaatcg tagggtagaa cttgaatgaa tggttgaaag gaggaaaatg gaatcgatga
     3481 aaattgccgt tgaacaacct tatgaagacg tcatgaacgc actcttggct aaaggttatg
     3541 aagccaagat gtttaacacc gatgaagatg tcaccggcta cgacatcggc gtcgtccgtg
     3601 ccatcaatga agggaacacg catgaattca actttccggt cattacgatg aagggcatgt
     3661 cgattgacga cgttgtccac gcagttgagc agaaagcgaa cctcctccaa taagcatccc
     3721 cgaaataaca gaatctatgg caccctccgt agattctgcc tttcatgaac atgacagccc
     3781 gccaattgac ctgattggcg ggctgttttg atcaatggaa a
//
LOCUS       DASULI010000057         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_219264, whole
            genome shotgun sequence.
ACCESSION   DASULI010000057 DASULI010000000
VERSION     DASULI010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219264"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFX34_01260"
     CDS             complement(<1..436)
                     /locus_tag="VFX34_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX5563559.1"
                     /translation="MRQREQWTSKIGFVLAAAGSAIGLGAIWKFPYMAGTNGGSVFVL
                     LFIICTLAIGLPILLAEFVIGRRGQHDAVTSLKKLAPGKPWFFIGWMGLLSSFILLSF
                     YSVVGGWILSYLARSLFFRLDVADYGGLFNAIIANPGEVLIAQ"
     gene            589..1086
                     /locus_tag="VFX34_01265"
     CDS             589..1086
                     /locus_tag="VFX34_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX5563560.1"
                     /translation="MELIKVNGRIDRKRLAVGIVMPIIGGSITGWLANRNAQEKYKKL
                     KKPSFSPPPSTFPIAWTTLYGLMGLAKYRVEKKKELGQGNKSAIPIYELQLGLNYLWS
                     FLYFRWGLRGTALVEMTILLAAIALTTYEFYQVDRTAGSLMIPYIGWVVFALSLNYST
                     WKMND"
     gene            complement(1133..1546)
                     /locus_tag="VFX34_01270"
     CDS             complement(1133..1546)
                     /locus_tag="VFX34_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5563561.1"
                     /translation="MDAKETAGKILEESYVGTMATVQQGKPYSRYMTFFNEDFTLYTA
                     TSKETEKVDELEKNPNTHILIGYEGEGFGDEYLEIMGTVTISDEESLKEKVWNEHMKP
                     YFNGPDDPNLVILKITPESMRLMNKKGEAPQEIQF"
     gene            complement(1642..2151)
                     /locus_tag="VFX34_01275"
     CDS             complement(1642..2151)
                     /locus_tag="VFX34_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007084707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5563562.1"
                     /translation="MKIRRLTPPDAEAYFQLRLEALQTNPEAFSSSYEEERKQTADKY
                     KDRFQGGNSFTFGAFNESKLIGVITLLTEQTMKLGHRATIVAMYVTPGMRGKGIAKKL
                     MNEAISKARAMKGIEQIHLTVVSSNATAIQLYSSLGFEAYGTEKRALKIGKDYFDEDF
                     MVLFLHDIN"
     gene            2277..3437
                     /locus_tag="VFX34_01280"
     CDS             2277..3437
                     /locus_tag="VFX34_01280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5563563.1"
                     /translation="MSKELFITTDFQREWMDELSKLEQQFRSSAEQTDRLAEFPKENI
                     QSLVEMGYTTLALPKEYGGGGMTVTDMILMQETIASYDGATGLSIGWHQGVVGDLYEN
                     GLWEKNNLKRFAREILNGSLVNRAASEAQTGSPTRGGRPQTTAVKEDGHWVINGQKNF
                     TSMSPVLTHTLVSAWVEEREGIGFFLIDQTREGVSIKETWNMVGMRGTESHDLVLDNV
                     KVDEEDFVEFSKGRRGDKLNGWLLHVPACYLGIAQAARDYAVKFAVEYSPNSIRGTIS
                     DLPNVQAHIGQIDLELMKARHFLYSVAAAYDNLEQRTSMNQELGAAKHVVTNAAIRIV
                     DQAMRIVGAKSLDLSNPLQRYYRDVRAGLHNPPMDDMVISKLAQAAIEDMGK"
     gene            3434..3991
                     /locus_tag="VFX34_01285"
     CDS             3434..3991
                     /locus_tag="VFX34_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEX5563564.1"
                     /translation="MIKRLVITGYKAHELGIFNDKHPGIGIIKKALEDRLTPLLDSGL
                     EWVIVSGQPGVETWAAETVIDLQDMYPELKFAVITPFEEQEKNWNEAKKAAYEFLLAH
                     ADYSVSLTKRPYEAPWQFIERDKFLLRNSDGILIVYDEDNEGSPKFMKRMALEFAEKS
                     HYEVLTISADDLQLVAEDMQRQDWD"
     gene            complement(4216..>5248)
                     /locus_tag="VFX34_01290"
     CDS             complement(4216..>5248)
                     /locus_tag="VFX34_01290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5563565.1"
                     /translation="NKDNGEFYFITSKGVVDKLYRYHLEDGKLVESTLPVDVIDQVRF
                     AKSGNLYILGRSATVPHNIFKSKDGQEWEQLTENRVLGVSQEGMVEPEVVSYSSFDGL
                     EIEALLFKAKEENANGYTIFWPHGGPQSSERKMFRSMFQCFLNRGYNIFAPNFRGSTG
                     YGSEFTKMVEQDWGEGPRLDCLAGIDWLFDNGISDRERLFLVGGSYGGYMALLLHGRH
                     AEYFKAVVDIFGVSDLFTFVNSVPPHWKPIMDRWLGDPERDKERFVKDSPVTYLDGMV
                     KPMLVIQGAKDPRVVKEESDQIVAKLTDLGRDVEYLVLDDEGHGFSKKENEITVYSRM
                     LEFLEKHQK"
BASE COUNT         1466 a         1217 c         1139 g         1426 t
ORIGIN      
        1 cctgcgcgat cagcacttct cctggattcg caatgatcgc attgaaaagc ccgccataat
       61 cggcaacgtc aagacggaaa aacaatgatc ttgccaaata ggaaagaatc cacccgccga
      121 cgacgctata aaaggataac aggataaagg agctaagcag tcccatccaa ccgatgaaaa
      181 accatggctt accaggcgcc aatttcttta aagatgtgac agcatcatgc tgcccccttc
      241 tcccgatgac aaattcggcc agcaggattg gcaatccaat tgccaacgta caaatgatga
      301 atagtaatac aaatacgctt ccgccgttcg ttcccgccat ataaggaaac ttccaaatgg
      361 caccaagtcc gatcgcactt ccggcagccg ccaatacaaa tcctattttc gatgtccatt
      421 gctcacgttg tctcatacaa tcactcccca atttctccga tgaacattgt aatagtatag
      481 cgtagaatgg atagaatcca tactatctaa ttattgtgca ttttccactg tttttgtttt
      541 attatctcgc taaagggaaa tatgtaagta attagggagg ttgaactatt ggaacttatc
      601 aaagttaatg gacggatcga taggaagcgg ttagctgtcg gcattgtaat gcccattatt
      661 ggcgggtcga taacggggtg gctggcaaac cgaaatgcac aggaaaagta taaaaaactc
      721 aaaaagcctt ctttctcccc acccccatca acattcccga ttgcatggac aaccttgtac
      781 ggattgatgg gactcgcgaa gtaccgggtt gaaaagaaaa aggagctagg gcaagggaat
      841 aaaagtgcaa tccccattta tgaattgcag ctcggcctca attatttatg gtctttctta
      901 tacttccgat gggggttgag aggtacagcc cttgtcgaaa tgaccatctt acttgcagcc
      961 atcgcattaa cgacgtacga attctaccaa gttgaccgga cagcgggctc actaatgatt
     1021 ccttatatcg gatgggttgt tttcgctttg tccctgaatt attctacttg gaaaatgaat
     1081 gattgagttt ccaagaagtc ccttgcctag aaaaaaggca agggacttcc aattaaaatt
     1141 gaatttcttg gggtgcttct ccctttttat tcatcagacg catggactca ggagtgattt
     1201 tgaggatgac gaggttcgga tcatcgggtc cattaaaata cggcttcata tgctcattcc
     1261 aaaccttctc tttcaagctt tcctcatcgg atattgttac ggttcccatg atttccagat
     1321 actcatcccc aaacccttct ccttcatagc caatcaaaat atgcgtattc ggattctttt
     1381 cgagctcatc cactttttcg gtctcttttg aggttgccgt gtaaagcgtg aaatcctcat
     1441 taaaaaacgt catatacctg ctgtatggct tgccttgctg aacagtcgcc atcgttccga
     1501 catagctttc ttccaaaatt ttgccagccg tttcttttgc atccattagc aatcactcct
     1561 catttattgt ctctcctaac tgttcctata taaaagggaa actaaacatc gaaggctttg
     1621 gcaaaatatt tctgataagc tctaatttat atcgtgtaag aataacacca taaagtcttc
     1681 atcgaaataa tcctttccta ttttcaacgc tcttttttcc gttccgtacg cttcaaagcc
     1741 gagtgatgaa tacaattgaa tagcagttgc attcgagctg acgactgtta agtgaatctg
     1801 ctcaattcct ttcatggctc ttgccttgct gatcgcttca ttcatcagtt tctttgcaat
     1861 acctttcccc cgcattccag gtgtcacgta cattgctacg attgttgcac gatgcccgag
     1921 tttcatcgtt tgttctgtta acagtgtaat gaccccgatc agcttcgatt cgttaaatgc
     1981 gccaaacgta aaggaattcc ctccttgaaa cctatccttg tacttgtcag cagtttgctt
     2041 tctttcctct tcataacttg aagaaaacgc ttctggattt gtttgcaaag cctctaatcg
     2101 aagttgaaaa taagcttcag catctggtgg tgttaatctt ctaatcttca tctatatcct
     2161 cctccatcta ttgaacagat acattcattt tacctttcaa ttcaattttc ctgcaatcac
     2221 caactagtaa cgtataatag acatacataa gtacgttaac taaaggatgt gtcgatatgt
     2281 caaaggagct tttcatcaca actgacttcc aacgggaatg gatggatgaa ttaagcaagc
     2341 ttgaacagca atttcggagc tcggctgaac agacggatag acttgctgaa tttccgaaag
     2401 aaaatataca gtcgcttgtt gaaatgggct acacaacact tgcactgccg aaagaatatg
     2461 gcgggggcgg aatgactgtg accgatatga ttctcatgca ggagacgatc gcttcctacg
     2521 atggggcaac cgggttatcc atcggatggc atcagggcgt tgtcggtgac ctttatgaaa
     2581 atggactatg ggaaaagaac aatttaaagc ggtttgcaag agagattttg aacggctcct
     2641 tagtgaaccg cgcagcaagt gaagcgcaaa ccggcagccc gacgcgcggt ggccgtccgc
     2701 aaacgacggc ggtcaaagaa gatgggcatt gggtcattaa cgggcagaaa aatttcactt
     2761 ccatgtcgcc tgtcctcact cataccctcg tttcggcatg ggttgaagag cgagaaggaa
     2821 tcggattttt ccttattgat caaacaaggg aaggtgtatc aatcaaagag acatggaata
     2881 tggtcggcat gcgagggacg gaaagccatg acttagtttt agataatgtg aaagtcgatg
     2941 aggaagactt tgtcgagttc agcaaaggac gtcgcggaga taaattgaac ggctggcttc
     3001 ttcatgttcc tgcctgctat ttagggattg cgcaagccgc ccgtgactat gcggtgaaat
     3061 ttgctgtcga atatagcccc aatagcatcc gagggacgat ttccgatttg ccgaatgtgc
     3121 aagcacatat tgggcaaatc gatttggaat taatgaaagc gcgccatttc ctatacagtg
     3181 ttgcagctgc atacgacaac ttggaacagc gcacgtcgat gaatcaagaa ttaggagcgg
     3241 caaagcatgt cgtgacaaat gcagcaatcc gcattgtcga tcaagcaatg cgaatcgtcg
     3301 gtgcaaaaag cttggatcta tcgaatccat tacaacggta ctaccgggat gtccgggcag
     3361 gattacataa tccgcctatg gatgatatgg tcatttcaaa attggcgcaa gccgccatcg
     3421 aggatatggg caaatgatca aaagactagt tatcacaggt tataaagcgc atgaactagg
     3481 catcttcaat gataagcatc caggcatcgg catcattaaa aaggcgctgg aagaccgatt
     3541 gactccactg cttgacagcg gactcgaatg ggtgattgtc agcggacaac cgggagtcga
     3601 gacatgggct gcggaaacag tgatcgactt gcaagatatg tatccggaat tgaaatttgc
     3661 tgtcattacg ccatttgaag agcaggagaa aaactggaat gaggcgaaaa aggcagcgta
     3721 tgaatttctc ctagcacatg cggattactc ggtcagttta acgaaacgtc catatgaagc
     3781 tccgtggcaa ttcatcgagc gggataagtt cttattgcgg aattcagacg ggatcttgat
     3841 cgtttacgat gaggacaatg aagggtctcc aaaattcatg aagagaatgg cattggaatt
     3901 tgccgagaag agtcattatg aggtgctgac gatttcagcg gatgacttgc agttagttgc
     3961 cgaggatatg cagaggcagg attgggatta agcaaaaaat catagcataa ctcatctttc
     4021 ttggctggag agacaggtat atgatcactt attcggattt atgagtactt accacgtttt
     4081 atgagcactt atccggattt atgagcactt accgcgtttt atgatcactc atcttgatta
     4141 atgggcgaaa actaaacctt tccatagtaa aagctgtccg cagtcgttgc atgactggcg
     4201 gacagctttt cattatcact tctgatgctt ctccaaaaac tccagcatcc ggctgtatac
     4261 tgtaatttca ttttccttct tcgagaagcc atgcccttca tcatccaaca caagatattc
     4321 aacgtcgcga cctagatctg tcaacttcgc gacgatctga tcggattcct ctttgacaac
     4381 acgcgggtcc ttcgcacctt ggatgaccag catcggcttt accattccgt caagatacgt
     4441 gacgggcgaa tctttgacga atcgctcctt atcccgctcc ggatctccta gccaacggtc
     4501 catgatcggc ttccaatgcg gcggaacgga attgacgaat gtgaaaagat cggacactcc
     4561 aaaaatatcg actaccgctt tgaagtattc ggcatgacgt ccgtgaagca gcaatgccat
     4621 atagccgcca tagctgccgc ctacgagaaa tagccgctcc cggtcggaaa tgccattgtc
     4681 aaacaaccaa tcgatccccg ccaagcagtc aaggcgcggg ccctctcccc aatcctgttc
     4741 gaccattttc gtgaattcag agccatagcc ggtgcttccc cggaagttcg gggcgaaaat
     4801 gttatagccc ctattcagga agcattggaa catcgaacgg aacatcttgc gttccgatga
     4861 ctgcggacca ccatgcggcc aaaagatcgt gtagccattc gcattttcct ctttcgcttt
     4921 gaataaaagt gcttcgattt ccaatccgtc aaacgacgaa taggaaacaa cttctggttc
     4981 aaccatgcct tcttgactga cccctaacac cctattttca gtaagctgct cccactcttg
     5041 tccatctttc gatttgaaaa tattatgagg gacggtagca ctgcgaccta aaatatagag
     5101 atttcctgac ttggcgaatc ttacttgatc aattacatcg acaggcaatg tgctttcgac
     5161 cagcttccca tcttcaagat gatagcgata taatttatcc actacaccct tggatgtgat
     5221 gaaataaaat tccccgttat ctttattg
//
LOCUS       DASULI010000058         4450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_298704, whole
            genome shotgun sequence.
ACCESSION   DASULI010000058 DASULI010000000
VERSION     DASULI010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298704"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFX34_01295"
     CDS             complement(<1..432)
                     /locus_tag="VFX34_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HEX5563566.1"
                     /translation="MEIVGWIAAILMFIVAFVGLIYPIIPSVFFIVGGFLLYGLFASF
                     DEMSWLFWVIQALFVILLFGADTLANVVGVKKFGGSKAGMWGSTIGLLAGPFVIPVAG
                     ILIGPFLGAFLAELLVTRTGVKQSLKTGIGSIVGFFTSIAAK"
     gene            complement(446..2077)
                     /locus_tag="VFX34_01300"
     CDS             complement(446..2077)
                     /locus_tag="VFX34_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX5563567.1"
                     /translation="MEVNWQEVIFQFIGGLGIFLFAIKYMGDGLQKAAGDRLRSILDR
                     FTTNPFMGVLVGIIVTVLIQSSSGTTVITVGLVSAGFMTLRQAIGVIMGANIGTTITA
                     FIIGLDVGAYALPIMALGAILIFFFKKNAVKNVGEVVFGFGGLFLGLELMSAGMKPLR
                     SLTAFSDFTVSMADHPVLGVVAGTVFTLIVQSSSATVGILQGLYAENLVSLQAALPIL
                     FGDNIGTTITAVLASIGASVAARRAAATHVLFNVVGTVVFMILLYPFTLYVEWISGLL
                     ALESKMQIAFAHGTFNVLNTVLQFPLIGAWALLVTKLIPGKDVTIEYRPKHLDPNFIN
                     QSPSVAIGQAKEEIIRMGDFAVQGMEETYEYLKTSNKRHAETAYQIEDAINNLDRKIT
                     DYLVDISAVNISPVESARHVMLMDTVRDIERIGDHFENIIELIDFREVNRVKLTDEAM
                     NDLTEMFTLTIATVDKAVRSLDMNDIELAREVAEQEALIDKMERKFRKNHIVRLNEGH
                     CSAQSGMVFVDIVSNLERIGDHAVNIAEAILGKRV"
     gene            2279..2782
                     /locus_tag="VFX34_01305"
     CDS             2279..2782
                     /locus_tag="VFX34_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HEX5563568.1"
                     /translation="MKFHQLFLAAFHEPKKLAAFRLLPIGRVIRYAFLFILLMTLISF
                     TRFSLGDAELFDASPELTEYSKTIGGLIYPMAFIFQFVIATFYVFVRISLFAGIGIWI
                     GNVMKRRIEYRFIWRTTAIAITVPLLLTIILDFFQFWETSGILISSFIHIGYIIAALK
                     YYPKAAK"
     gene            3247..4371
                     /gene="ispG"
                     /locus_tag="VFX34_01310"
                     /gene_synonym="gcpE"
     CDS             3247..4371
                     /gene="ispG"
                     /locus_tag="VFX34_01310"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5563569.1"
                     /translation="MSEIIHRSKTRPVRVGNLTIGGSDELFIQSMTTTKTHDVDATVE
                     EILRLEEAGCQIVRVACPDERAAYSIGAIKKRINIPLVVDIHFDYKLALIAIEQGADK
                     IRINPGNIGRQEKVEAVVKAAKAKGIPIRIGVNAGSLEKHILKKYGYPTADGMVESAL
                     HHIKILEDLDFHDIIVSLKASDVNLAVEAYMKAAAAFDYPLHLGITESGTLFAGSIKS
                     AAGLGTLLSAGIGNTLRVSLSADPTQEIKVARELLKIFGLSSNAATLISCPTCGRIEI
                     DLISIANEVEEYISTIKAPLKVAVLGCAVNGPGEAREADIGIAGARGEGLLFMKGKTV
                     RKVPEETMVEELKIEIDKLAEEYFEKKRQEELLLQSGASE"
BASE COUNT         1238 a         1076 c          964 g         1172 t
ORIGIN      
        1 tttggcggct atcgaagtga agaagccgac aatcgatccg attcccgtct tcaatgactg
       61 tttcacaccc gtccttgtca caagcagttc cgccagaaaa gcgccgagga agggcccgat
      121 aaggattcct gccacaggaa tgacgaaagg tcctgcgagc aggccaatcg tacttcccca
      181 catcccagcc tttgaaccgc caaacttctt tacacctacg acattcgcta gcgtatccgc
      241 gccgaacagc aagatgacga acaacgcctg gatcacccaa aacaaccaac tcatctcatc
      301 gaaggaagca aataatccgt acagcaggaa accgcctacg atgaaaaata cggaagggat
      361 gattggatag atcaacccga caaaagcgac gatgaacatg agtattgccg ctatccagcc
      421 tactatctcc atccctttcc ccccattaca cccgtttgcc taaaatggct tcagcaatat
      481 tcacagcatg gtcgccgatt cgctcaagat tggatacgat atcaacaaag accattcctg
      541 actgcgcgga acaatgccct tcattcaaac ggacaatatg atttttacgg aatttgcgct
      601 ccattttatc aatcaacgct tcttgctcgg caacttccct cgccaattca atatcattca
      661 tgtccaatga acgtaccgct ttatcgacag tcgcaattgt caacgtgaac atctccgtca
      721 aatcattcat cgcttcatct gtcaatttca cccggttcac ttcacggaaa tcaatcaact
      781 cgataatatt ttcgaaatga tccccgatcc gttcaatgtc ccgaactgta tccatgagca
      841 tgacgtgcct tgcagactcg accggagaaa tattgaccgc ggaaatatcc actaggtaat
      901 ccgtaatttt ccgatccaga ttattgatgg catcttcaat ctgatatgcc gtttcagcat
      961 gtcttttatt gctcgtcttc aaatactcat aggtttcttc catgccttgg accgcgaaat
     1021 cacccatcct gataatctct tcctttgctt gtccaattgc gacggacgga gattggttga
     1081 taaagttcgg atccagatgc ttcggtctgt attcgatagt gacatccttg cccggtatga
     1141 gctttgtcac tagcaatgcc caagcaccaa tgagcggaaa ttgtaaaaca gtgttcagca
     1201 cattgaacgt accatgggca aatgcgatct gcattttact ttcaagagct aaaagtccgg
     1261 aaatccattc gacgtatagt gtgaaaggat atagtagaat catgaaaacg actgtcccga
     1321 caacattaaa gaggacatgc gtagccgcag cccttctagc cgcaacagat gcgccgatcg
     1381 atgcgagcac cgctgtaatt gttgtaccga tattatctcc gaagaggatt ggtaaagctg
     1441 cttgtaatga aacaaggttt tctgcgtata acccttgcaa aatcccgaca gtcgcactgg
     1501 agctctgaac gataagtgta aatacagttc ctgctacaac cccgagcacg ggatggtcgg
     1561 ccattgatac tgtaaaatca gagaatgccg taagggacct tagcggcttc ataccggcac
     1621 tcatcagttc caaaccgagg aagaggcctc cgaatccaaa tacaacctcc cccacgttct
     1681 tgacggcatt ctttttaaag aagaagataa ggattgcacc gagtgccatg atcggcaatg
     1741 catatgcccc gacatcaagg ccgataatga atgccgtaat tgtcgttccg atgttcgcac
     1801 ccatgatgac gccgattgcc tgtcgcaggg tcatgaagcc ggcactgacc aatccgaccg
     1861 taatgacagt cgtcccggaa ctcgattgaa tgagcacagt cacgataatc ccgacgagga
     1921 cgcccatgaa cggatttgtc gtgaatctgt ccaatatgct gcgcagtcta tcccctgcgg
     1981 ctttctggag accgtcaccc atatatttta tggcgaatag gaaaatcccg agacctccta
     2041 taaattgaaa gatcacttct tgccaattta cttccattac aatctctacc tccatcatgc
     2101 tttatgtttt agcattccta ttatgttgat tttattaatg aattgtaaat agaagtgggt
     2161 gaatctttac attcccttaa caatagacgg ggaaaaagtc atcattctgt cgaattttga
     2221 attcgcgctt cacaggtcgg gacatgatag actatcaata tgtaaaggag gtcctgtttt
     2281 gaagttccat caactatttc tggcagcgtt ccatgaaccg aaaaaactcg cggcattccg
     2341 tctgctcccg atcggtcgtg tgatccgcta cgctttccta ttcatccttt taatgacatt
     2401 gatttcattc acccgctttt cactcgggga tgctgaattg ttcgatgcat cacccgagct
     2461 gaccgagtat agcaaaacga ttggcggact gatttatccg atggccttca ttttccaatt
     2521 cgtcattgcg acattttacg tatttgtcag gatcagcctc tttgccggaa tcggcatatg
     2581 gatcggcaat gtgatgaaaa gacgtatcga ataccgtttc atttggcgga cgaccgccat
     2641 cgcaattact gtccccttgc tgctgacgat tattctggat ttctttcagt tctgggaaac
     2701 ttcgggcatt ctgatttcgt cctttatcca tatcggttac ataattgctg cactcaagta
     2761 ttatccaaaa gcagccaaat gaatactttc ccaaatcccg catattgtgt gggaaggggg
     2821 aatgttcatg cgcactttac tcgtagctgt aatcctgttt ctaacctttc atattatccg
     2881 gattgactta ggggatggga caatccctct agcatctttt ttcgggaatg aaccgccttg
     2941 taccgagacg accacagtga aaaccatata tgtcacgaca atccaaggcg atacgattga
     3001 atccctattt tcaatgtacc cggatgccga tactacattt gtagaaagat tatcaatttt
     3061 ttattccctg aatcctcatc ttcgaaacca ggagcttgtt ggcggtcttt ccgtggagct
     3121 tcccctttcg aaagaaggtc aggaaaattg tgaatgaaga tgttttaagc ggtgtctctt
     3181 gtcttttcat ccaattgatt gctaaaatag aatcagttat gagtttttta aggagagaat
     3241 atatccatga gtgaaataat acacagatct aaaacacgtc ctgtacgcgt cggcaatttg
     3301 acgatcggcg gcagcgacga actgttcatc caaagtatga caacaacaaa aacgcatgac
     3361 gtcgacgcaa cggtggaaga aattttacgt cttgaggaag cgggctgtca aatcgtccga
     3421 gtagcttgtc ccgacgaaag ggcagcttac tccatcggcg ccatcaaaaa acggatcaat
     3481 atcccgctcg tcgtcgatat ccatttcgac tataagcttg cattgattgc aattgaacaa
     3541 ggcgcggata aaatccggat caacccgggc aacatcggcc gtcaggaaaa agtggaagct
     3601 gtcgtcaaag cggcaaaagc aaaaggtatt ccgatcagaa tcggagtgaa cgccggctca
     3661 cttgagaagc acatcttgaa aaagtacggt tatccgactg ctgacggaat ggttgagagt
     3721 gctttgcatc atattaaaat attggaagat ctagatttcc acgatattat cgtttctctg
     3781 aaagcttcgg atgttaactt ggcagtagaa gcatatatga aagcggcagc cgcattcgat
     3841 tatccattgc atctaggaat cactgaatcc ggcacattgt tcgccggttc catcaaaagt
     3901 gctgcaggtc ttggaacctt gctctcagcc ggcatcggga atacgctccg tgtttcattg
     3961 agcgcggatc ctacccaaga aataaaagtt gcgcgcgaac tattgaagat attcggtcta
     4021 tcctccaacg ctgcgacgct catctcttgc ccgacttgtg gacgtatcga gatcgacttg
     4081 atttcgattg cgaatgaggt ggaggaatac atttccacta ttaaagcacc attgaaagtc
     4141 gctgtattgg gctgtgcagt taacggtcca ggagaagcgc gtgaagcgga catcgggatt
     4201 gcaggtgcac gcggggaagg attgctcttc atgaaaggga aaacagtccg gaaggtgcct
     4261 gaggaaacaa tggtcgaaga gttgaaaatt gaaattgata agttagccga agagtatttt
     4321 gaaaagaaac gccaagaaga actgcttctt caaagcgggg catcagaatg aggaaaatcc
     4381 gattcggcat tgatatagat gggactgtta catgtccgac ttcccttctt ccccatatca
     4441 atgcacaatt
//
LOCUS       DASULI010000059         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_259712, whole
            genome shotgun sequence.
ACCESSION   DASULI010000059 DASULI010000000
VERSION     DASULI010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259712"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFX34_01315"
     CDS             <1..223
                     /locus_tag="VFX34_01315"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426988.1"
                     /note="E3 component of the branched-chain alpha-keto acid
                     dehydrogenase complex; catalyzes the oxidation of
                     dihydrolipoamide to lipoamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5563570.1"
                     /translation="DGFVKIIADKKSDDILGVHMIGGHVTDLISEAGLAMVLDATPWE
                     VASTIHPHPSLSEVIGEASLAVDGIAIHM"
     gene            301..1308
                     /locus_tag="VFX34_01320"
     CDS             301..1308
                     /locus_tag="VFX34_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX5563571.1"
                     /translation="MTKARHEELGLTNEDVVEIYETMVMARRLDERMWLLNRAGKIPF
                     VISCQGQEAAQAGAAFALDKERDWIAPYYRDMGVVLHFGMTPKELMLSAFAKAEDPNS
                     GGRQMPGHFGQRKNRILTGSSPVTTQLPHAVGVALAAKMKNEDFITFVTLGEGSSNQG
                     DFHEGMNFAGVHKLPTVIMVENNKYAISVPVEKQLACENVSDRAIGYGMPGVTVDGTD
                     PLEVYKVVKEAADRARKGEGPSLVETICYRLTAHSSDDDHRLYRDPEELENERKLDPI
                     PKFAAYLRETGVIDEAYEKEMEERIMAIVNEATDYAEKAPYAEPEYALRHVYKEEGGN
                     E"
     gene            1308..2291
                     /locus_tag="VFX34_01325"
     CDS             1308..2291
                     /locus_tag="VFX34_01325"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5563572.1"
                     /translation="MAVISYIDAITLAMKEEMERDENVFVLGEDVGRKGGVFKATTGL
                     YDQFGEYRALDTPLAESAIAGVGIGAAMYGMRPIAEMQFADFIMPAVNQIVSEAAKIR
                     YRSNNDWSCPIVIRAPFGGGIHGALYHSQSVESMFASTPGLKIVIPSTPYDAKGLLKA
                     AIRDDDPVLFFEHKRAYRLIKGEVPEEEYVLPIGKADVKREGDDITIITYGLCVHFAL
                     QAAERLAEDGISAHILDLRTVYPLDKEAIIEAASKTGKVLLVTEDNLEGSIMSEVSAI
                     IAENCLFDLDAPIKRLAGPDIPAMPYAPTMEKFFMVNPDKVEKAARELAEF"
     gene            2312..>2783
                     /locus_tag="VFX34_01330"
     CDS             2312..>2783
                     /locus_tag="VFX34_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5563573.1"
                     /translation="MTIEKIEMPKLGESVTEGTIEKWLVKPGDTVNKYDPLAEVNTDK
                     VTAEIPSSFAGTIKEIIVQEGETVEVGIVVCTIETEGGTEAPVEAVPAKEEPANEMPA
                     AGSQKPAAPIKREAGAARYSPAVMRLAQENNIDLSLVQGSGKEGRITRKDLQRII"
BASE COUNT          817 a          603 c          725 g          638 t
ORIGIN      
        1 tgacggcttt gtcaaaatca tcgccgataa aaaatccgat gatattctag gcgtccatat
       61 gattggcggg catgttacag acttgatttc ggaagctggt ctcgcaatgg ttcttgacgc
      121 gactccatgg gaagtggctt caaccatcca tccgcatccg tcgctctctg aagtgatcgg
      181 ggaagcatcc cttgcggtgg atggcatagc aattcatatg taatacaatt aatcggcatc
      241 ggctgtccgt gtaaaaccga acaccgatcc tgtgacttgg aatagaaggg ggtttcaaac
      301 atgactaaag cacgtcatga agaactggga ttgacgaatg aggatgtagt ggaaatatat
      361 gaaacgatgg tcatggcaag acgtttggat gagcgtatgt ggctattgaa tcgtgcgggt
      421 aaaatcccgt ttgtcatttc atgtcaaggg caggaggcgg cccaagctgg cgctgcgttt
      481 gcacttgata aggaaagaga ttggattgca ccttattacc gtgatatggg cgttgtcctc
      541 cattttggaa tgacaccgaa ggaattgatg ctttcggcat tcgcaaaagc ggaagacccg
      601 aactccggcg gccgccaaat gccaggacac ttcggacaac ggaaaaaccg tattctgact
      661 ggatcatccc ccgtcacgac acagcttcca catgcagttg gtgttgcatt ggctgcgaag
      721 atgaaaaatg aagatttcat cacattcgtc acgttagggg aagggtcgtc caaccaaggc
      781 gatttccacg aaggaatgaa ctttgcaggc gtccacaaat tgccgacggt catcatggtc
      841 gaaaacaata agtatgccat ctcagtgcca gttgaaaaac agctcgcttg cgaaaatgtt
      901 tccgatcgcg ctattggata tggcatgcca ggtgtaactg tagacggaac agatccgctc
      961 gaagtataca aggtagtaaa ggaagccgct gatcgtgcac gcaaaggtga aggtccaagc
     1021 cttgttgaaa cgatctgcta ccgtttgacg gcgcattctt cagatgatga tcaccgtcta
     1081 tatagagacc ctgaagaact tgaaaacgaa cgaaagcttg atccgattcc taagttcgcg
     1141 gcttatttac gtgaaacagg cgtcattgat gaagcttatg aaaaagaaat ggaagaacgt
     1201 attatggcaa tcgtcaacga agcgaccgat tatgctgaaa aagcacctta tgcagaaccg
     1261 gaatatgcgc ttcgccatgt atataaagaa gaagggggga acgaataatg gccgtcattt
     1321 catatattga tgcaatcaca cttgcgatga aagaagaaat ggaaagagac gaaaatgtat
     1381 tcgttcttgg agaagacgtt ggaaggaaag gcggcgtttt taaagcgact accggacttt
     1441 acgatcaatt cggtgaatac cgtgcacttg atacaccgct tgctgaatcc gcaatcgcgg
     1501 gtgttggaat cggagctgcc atgtatggca tgcggccgat cgctgaaatg caattcgccg
     1561 atttcatcat gccggctgtg aaccaaatcg tatctgaggc cgcaaaaatc cgctatcggt
     1621 ccaataacga ctggtcttgt ccgatcgtga tccgtgcgcc attcggcggc ggtatccatg
     1681 gcgctttata tcactcccaa tcagttgagt ccatgtttgc aagtactcca gggctgaaaa
     1741 tcgtcatccc ttcaactcct tacgacgcaa aagggctgtt gaaagcggcg atccgagatg
     1801 atgatccggt tctcttcttc gaacataaac gggcttaccg attgatcaag ggagaagtgc
     1861 ctgaagagga atatgtactg ccaatcggaa aagcggatgt gaagcgtgaa ggggatgata
     1921 ttacaatcat cacttacgga ctatgtgtac acttcgcttt acaggcagcg gaaagactgg
     1981 ctgaggatgg aatttccgca catatcctcg acctgcgcac cgtctacccg ttggataaag
     2041 aagcgatcat tgaagcggca tcgaaaacgg gtaaagtgtt acttgtcact gaagacaatc
     2101 tggaaggcag catcatgagt gaagtctctg caatcattgc agaaaactgc ctattcgatc
     2161 ttgatgcacc gatcaagcgt cttgctgggc cggatatccc ggcaatgcca tacgcaccga
     2221 cgatggagaa gtttttcatg gtcaacccgg ataaagttga aaaagctgca cgtgaactcg
     2281 cagaatttta atcggaaagg aggggtatgt tatgacaatt gaaaaaatcg aaatgccgaa
     2341 acttggagaa agtgtgacag aaggaacaat cgaaaaatgg ttagtaaagc cgggggacac
     2401 tgtcaataag tatgatccat tggctgaagt gaatacggac aaagtaactg ccgaaatccc
     2461 ttcttctttc gctggaacga taaaagagat tatcgtgcag gaaggcgaaa ccgtagaagt
     2521 tggaatcgtc gtctgtacga ttgaaacgga aggcggcaca gaagcacccg tggaagcagt
     2581 ccctgcaaaa gaagagccgg caaatgaaat gcctgcggct ggctcacaaa aacctgcagc
     2641 gccgattaaa cgtgaagcgg gagcagcacg ctactcccct gcggtcatgc gattggcgca
     2701 agaaaataat attgaccttt cacttgtcca aggttcaggg aaagaaggac gcatcacacg
     2761 gaaagatcta caaagaatca tcg
//
LOCUS       DASULI010000060         4517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1940581,
            whole genome shotgun sequence.
ACCESSION   DASULI010000060 DASULI010000000
VERSION     DASULI010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940581"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1543)
                     /locus_tag="VFX34_01335"
     CDS             complement(215..1543)
                     /locus_tag="VFX34_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEX5563574.1"
                     /translation="MHRKKVSFSKRVFTGLGLGILFGFILNIAYGADSEVLAKSVSWF
                     NIVGTGYVKLLQMIVVPLVFISILGAFTKVTIGKNFGKMAGMILGILVGTTAIAAAIG
                     ISASLLFGLDASEIVHGEAETARGVSIEERSAEMAGKALPDQILDLLPANPFLDFTGA
                     RPTSTIGVVIFAAFLGFAYLALTRRDEENANLVKKGIDALYSLIMGVVRIVLRLTPYG
                     ILAIIARTVATSDFAAIYSLGKFVLASYVALLVMFLIHLIIITVSGLNPITYVKKTAE
                     ALLFAFSSRSSAGTLPLNIKTQTDRLGVPDGIANFAGSFGLSIGQNGCAGVYPAMLAV
                     MIAPTVGQNPLEPTFLITLIAIVAISSFGVAGVGGGATFAAILVLSALNLPVALAGLL
                     ISVEPLIDMGRTALNVSGSMTAGVTTARINGELDKEIYNDSLEHQSIEGA"
     gene            1789..3240
                     /gene="cls"
                     /locus_tag="VFX34_01340"
     CDS             1789..3240
                     /gene="cls"
                     /locus_tag="VFX34_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX5563575.1"
                     /translation="MASLITITVTSIFILNIFLAIALIFLERRDPTATWAWLLVLFFV
                     PLFGFFIYLLFGRKLRKKHLFRWEGKNRIGIETLIDYQIESIEKGTFDFRLDDTAHYQ
                     DKIYLHLRNNHAVLTQDNDVQVFNDGVAKFDALLHDLENAKDHIHFQYYILRLDSLGT
                     RILEVLVKKAKQGVKVRVLFDDIGSRGLRIKHLKELIDNGGEVAAFFPAILPLINPRL
                     NYRNHRKIVVIDGRIGYVGGFNVGDEYLGLDKRFGYWRDTHLRIEGSALHPLQTRFIL
                     DWNQATEMEDIEYAERYFPAIPRKGSVGLQIVSSGPDAEWEQIKDGYLKMIFLAKEYI
                     YIQTPYFIPDVSFLDALRIACLSGVDVRIMIPNKPDHMFVYWATYSNVGKLLRAGAKV
                     YIYENGFIHSKQIVVDDELSTVGTANIDVRSFKLNFEINAFIYDREKSHELAELFEQD
                     MQLSTELTYEMYLKRTRWIKIKESVSRLLSPIL"
     gene            complement(3290..4258)
                     /locus_tag="VFX34_01345"
     CDS             complement(3290..4258)
                     /locus_tag="VFX34_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX5563576.1"
                     /translation="MNRKRKYKSGKKSNKALTITLLLTGLIMTAVVVWIGMNDWDVNK
                     TLRQAGIGETQAPPVEELEEDDLDKPEAIEEEPEAPEEEAPPVEEEKPVEEEPPAEVE
                     KPETKAPEKNVTPAKPAPKNEEKPKVTPKPPASPNKSGYIEGQELPDKPTIINGILLA
                     NKKHPLPSTYAPGESKEARAAFDEMAAEAKLSGFNLTAFSTYRSYEYQVSLYDRYVKR
                     DGVEAADRYSARPGYSEHQTGLAFDIGEVNFEKYWASSKFGETEAGKWVAANAHRYGF
                     ILRYPKGKESITGYMHESWHFRYIGKEVAEEIFNRNITLEEYLGLK"
     gene            4397..>4517
                     /locus_tag="VFX34_01350"
     CDS             4397..>4517
                     /locus_tag="VFX34_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5563577.1"
                     /translation="MNNQRWLSISFFTFFFTWGVFLPYWTGWLTIEKGLSVKDA"
BASE COUNT         1158 a         1140 c         1012 g         1207 t
ORIGIN      
        1 aagcgctccg ttcgattgac agtgcggtgg acaatatttc gcccatgccg caaacgcctt
       61 ataaagattt tattatggag acgcatgaat tgattcatcg ggaataaaaa cggggatgcc
      121 gcacgctttg gcgcgggatg gcttgctttg gacgtcaccc gcaaagaata aaaaaatgcc
      181 gtggaagcga catgcttcca cggcattttt taaatcaagc accttcaatc gattgatgct
      241 ccaatgaatc attataaatt tccttatcca actctccgtt aatccgcgca gtcgtcacgc
      301 ctgcggtcat ggatccactt acgttcaacg ccgtacgtcc catatcgatc agcggttcaa
      361 ccgaaatcaa cagacctgcc aatgcaaccg gaagattcaa agcggataaa acgaggatcg
      421 ctgcgaatgt agctccgcca ccgactccgg caactccgaa cgaactgatc gctacaatcg
      481 caatcaatgt gatcaagaat gtcggctcca atggattttg accgacagtc ggcgctatca
      541 tgacagcaag catcgctgga tagacgcctg cacagccgtt ctgtccgatc gacaatccga
      601 atgaccctgc aaagtttgca attccgtccg gaactccgag ccgatcggtt tgtgttttga
      661 tgttaagtgg taatgtacca gcactcgatc ttgaagagaa agcgaacagc aaagcttctg
      721 ccgtcttctt cacatacgtg atcggattca atcccgaaac cgtaatgatg atcaagtgga
      781 tcaggaacat gacgagcaat gcaacatacg aagcaaggac gaatttccct aagctataga
      841 tcgccgcaaa atccgaagtg gcaaccgtcc ttgcgatgat tgccaaaatt ccatatggcg
      901 tcaagcgcaa gacgatccgc acgactccca tgatcaatga ataaagggca tctatcccct
      961 tcttcaccaa attcgcgttc tcttcatccc gtctcgtcaa tgccaaatag gcaaatccga
     1021 ggaatgctgc gaagatgacg acaccgattg tcgatgtcgg gcgcgctccc gtgaaatcca
     1081 gaaacgggtt tgcaggtaag aggtccaaga tttgatccgg caatgccttg ccagccattt
     1141 ccgcggaacg ttcctcgatg gagacgccac gtgcagtttc cgcctcaccg tgaacaattt
     1201 cagaggcgtc caggccgaac agcaacgacg ccgaaatacc gatggcagcg gcaattgcag
     1261 tcgtaccgac aagaatccct aaaatcatcc ctgccatctt tccgaagttc ttgccgattg
     1321 tcactttcgt gaacgcacca agaatcgata tgaacacgag cgggacgacg atcatttgaa
     1381 gaagcttgac gtagcctgtt cctacgatgt taaaccagct gaccgatttc gccagcactt
     1441 ccgagtcggc tccgtaagcg atattcagga taaatccaaa aaggatccca agccctagac
     1501 ctgtgaaaac ccttttggaa aacgaaacct ttttccgatg catagtataa agaagtccta
     1561 ttaaaactaa caacactgct atatttacaa acagtaaaac tgtatccact tcttttcccc
     1621 ctccaggaat aaataaaaac tataaaaaga ctatataccc ttaaaccata tcagtcaagt
     1681 aggattaatt aattttattc catgaatagt caatgtggat gatattccct tctttttcag
     1741 gaggttttcg gtatactaat agtaagcagt cacataagga gggcatttat ggccagtttg
     1801 ataacgatta cggtcacttc cattttcata ttgaacatct tccttgcgat tgcgctcatt
     1861 ttcctcgaaa gaagagaccc caccgcaaca tgggcatggc ttcttgtcct attcttcgta
     1921 cctttattcg gctttttcat ctatttatta ttcggacgga aattacggaa gaagcacttg
     1981 ttcagatggg aagggaaaaa tcggatcggc attgagacgt tgattgatta ccaaattgaa
     2041 tcgatcgaga aagggacatt cgatttcaga ctggatgata cagcgcatta ccaagacaag
     2101 atttacctcc atcttcgcaa caaccacgct gttttgacgc aagacaatga tgttcaggtt
     2161 tttaatgatg gcgtcgcaaa atttgatgct ttgctgcatg atcttgaaaa cgccaaagac
     2221 catatccatt ttcaatatta cattttacgg ttggattcct taggcacccg cattctcgaa
     2281 gtgctcgtca aaaaagcgaa gcaaggcgtg aaagtgcgcg tcctgttcga tgatatcggt
     2341 tcacgcggac tccgcatcaa gcatttgaag gaactgatcg acaacggagg cgaagtggcc
     2401 gcattctttc ctgccatcct cccgctgatc aatccacggc taaactaccg gaaccaccgg
     2461 aaaatcgtcg tcattgacgg tcgcatcggc tatgtcggag gctttaacgt cggggatgaa
     2521 tatttagggc tggataaacg attcggctat tggcgcgaca cgcacctccg catcgaagga
     2581 agcgcactgc atccgctcca aacgaggttc atcctcgact ggaaccaagc gaccgaaatg
     2641 gaagacatcg aatacgcaga gcgatatttc cccgcgattc cacggaaagg ctcggtcggg
     2701 ctgcaaattg tatcaagcgg cccggacgcc gagtgggaac agatcaagga cggctatttg
     2761 aagatgattt tccttgcgaa ggagtatatt tacatccaga ccccgtactt cataccggat
     2821 gtcagtttcc ttgacgcctt gcgcatcgca tgtctatcag gggtggacgt ccgcatcatg
     2881 atcccgaata agccggacca catgtttgtg tactgggcga cgtattccaa tgtcggaaag
     2941 ctgttaaggg ccggcgcgaa agtgtatatt tatgaaaatg gattcatcca ctcgaaacag
     3001 attgtcgtcg atgacgaatt gtcgactgtc ggaacggcaa acatcgacgt acggagcttc
     3061 aaactcaatt tcgaaatcaa tgcgttcatt tatgaccgag agaagtccca cgagctcgcc
     3121 gaactgttcg aacaggatat gcaattgtcg acggagctga cgtacgaaat gtatttgaaa
     3181 cggacacgat ggatcaaaat caaagaatcg gtttcaagat tgctgtcacc gatcctgtaa
     3241 caagcaaaaa atcctcctct acccttttat taggataaag gaggattttt catttcagac
     3301 ccagatattc ttccaacgtg atgttgcgat taaagatttc ttcggcgact tcttttccga
     3361 tatagcggaa gtgccacgat tcatgcatgt agccggtgat gctttccttt cctttcgggt
     3421 accggaggat gaaaccgtaa cggtgtgcgt ttgccgccac ccatttcccc gcttccgttt
     3481 ccccgaattt ggaggatgcc caatattttt cgaagttcac ttcaccaata tcgaacgcga
     3541 ggccagtttg gtgctcggaa tagccggggc gcgcgctata acggtccgct gcctccacgc
     3601 cgtccctttt gacgtaccgg tcgtacaatg acacttgata ttcatatgaa cggtacgtac
     3661 tgaatgcggt cagattgaat ccggagagct ttgcctcggc cgccatctca tcgaatgccg
     3721 ctctcgcttc tttgctctca cccggagcgt atgtgcttgg caaaggatgc ttcttattcg
     3781 caaggaggat gccgttgatg attgtcggtt tatccggcag ctcctggcct tcgatatagc
     3841 cgctcttgtt cgggcttgcc ggcggtttag gggtcacttt cggcttctcc tcgttcttcg
     3901 gtgccggttt tgctggtgtg acatttttct ccggtgcctt cgtttccggc ttttccactt
     3961 cagccggcgg ctcttcttct acaggtttct cttcttcaac aggcggagct tcctcttccg
     4021 gcgcttcagg ttcttcctca atcgcttccg gtttgtccaa atcgtcttct tccagctctt
     4081 caacaggcgg agcttgcgtt tcaccgatgc ccgcttgcct taatgtttta tttacatccc
     4141 agtcattcat gccgatccaa acgacgactg ctgtcatgat caaacccgtc aaaagcagcg
     4201 tgattgtcaa tgccttgttg gatttcttac cgcttttata ttttcgtttc cggttcattt
     4261 ctattggcct tccttctact ctaggtatct ctcattttta tatatacata aatcgcccat
     4321 atgacgatgt tctttcaagt ccgtgtgtgc tatcatttat ttcacatctg taaatgaatg
     4381 attgcgagga ttacttatga ataatcaacg ttggctctcg attagcttct ttactttctt
     4441 ctttacgtgg ggtgtctttc tcccgtattg gacaggctgg ctgacgatcg aaaaaggatt
     4501 gtccgtaaag gacgcca
//
LOCUS       DASULI010000061         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1115455,
            whole genome shotgun sequence.
ACCESSION   DASULI010000061 DASULI010000000
VERSION     DASULI010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115455"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFX34_01355"
     CDS             <1..264
                     /locus_tag="VFX34_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX5563578.1"
                     /translation="NGIRSFMTFGGGDKEFVVVQEPAEAPGNQLAVSIEGDPVDLGFT
                     VAALTNNSIRWEQDGVAFFVASSTLTPDELIEVASSMHPEDSK"
     gene            337..1443
                     /gene="alr"
                     /locus_tag="VFX34_01360"
     CDS             337..1443
                     /gene="alr"
                     /locus_tag="VFX34_01360"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5563579.1"
                     /translation="MEHYRPTKAFVNLYAIQENLKSLKQYLPRHTSVIAVVKADGYGH
                     GEVEVAQAAIDAGARMVSVATPDEALHLRNGGIVADILVMGPSPVEFAEKAAALGIHV
                     TVSDAGWLRQAARMVEGTSHSLHVHVKIDSGMGRIGLRDENALQEIVEVVEGTSEITL
                     AGAFTHFACADEEDSGKTEDQFNRFMELVGKFPKRPPLVHASNSAATLLYPEYALDAV
                     RFGISLYGIAPSPYVGTKLPFGLKRAFTLETELAFVKKLEKGSAISYGGTYETVQDEW
                     IGTLPIGYADGLKRGLRGQEVLIGGERMPIVGTICMDQCMVKLPREFGVGEKVTLIGR
                     QGDQEITMEEWADRIGTIPYEIAVTIGKRIPRIY"
     gene            1606..1881
                     /locus_tag="VFX34_01365"
     CDS             1606..1881
                     /locus_tag="VFX34_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5563580.1"
                     /translation="MLELREKKIKIVKVSKGNEIEHTAVHQEPERGEFVYVSTKRYMT
                     DHEADTIREAMMIGYVEMSQINLRIAKECLHAELEAQHTVERLVSGG"
     gene            1886..2236
                     /locus_tag="VFX34_01370"
     CDS             1886..2236
                     /locus_tag="VFX34_01370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5563581.1"
                     /translation="MAIKRGDVFFADLSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIV
                     AAITAQIQKAKLPTHVEIDAERYGFERDSVILLEQVRTIDKSRLTDKITHLDEPLMER
                     VDEALEISFGLVKF"
     gene            2393..3223
                     /locus_tag="VFX34_01375"
     CDS             2393..3223
                     /locus_tag="VFX34_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5563582.1"
                     /translation="MNKIMVEGINQNMDEIIDRWIRRMKEEKGERFFHFMPDHLVEKT
                     SREFAVLMTSNIVESSAVNTERINEFTEKIVRFGWTIIFVNKAIDNFSEVVFQFLEEN
                     GIIKESNLRSFLLTFRNWINPLRESIIDAYSTEWERTVSLQKIALQELSASLIPVFDK
                     ISVMPLVGTIDTERAKLIMENLLEGVVSQRAEVVLLDITGVPVVDTMVAHHIIQAAEA
                     VRLVGAKCMLVGIRPEIAQTIVTLGINLHEFTTKSTLQKGMQAALLMTNRAIVEVEAK
                     "
     gene            3220..3582
                     /locus_tag="VFX34_01380"
     CDS             3220..3582
                     /locus_tag="VFX34_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5563583.1"
                     /translation="MNARIPILKLNEVLIVSIQWELDDQTAIQFQEDLLNKMHETAAL
                     GVVIDLTPIDFIDSFIAKVLGDVINMTGLMGAKVVITGIQPAVAITLIELGIRLSNVM
                     TALDLENGLDKLYKELEA"
     gene            3585..3986
                     /locus_tag="VFX34_01385"
     CDS             3585..3986
                     /locus_tag="VFX34_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX5563584.1"
                     /translation="MNFRSSVEIITEWDIVAARQLGRNEAKKAGFGTVDQARITTAIS
                     ELARNIYLYAGKGKIEIERVSEGGLFGIKIIASDEGPGIPDLRRVMEDGFSTSGGLGA
                     GMPGVKRLMDEFKVASEPGAGTIITTKKWLH"
     gene            4001..>4237
                     /locus_tag="VFX34_01390"
     CDS             4001..>4237
                     /locus_tag="VFX34_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase RsbU N-terminal domain-containing
                     protein"
                     /protein_id="HEX5563585.1"
                     /translation="MPQEVGRQYKEIMKKYLESHSEEDLYVGQQFSRRFIEKEIAPED
                     VISIHKSALFELHPELRNEVWDSLDFLIEMMIHYG"
BASE COUNT         1193 a          873 c         1183 g          988 t
ORIGIN      
        1 aacggaatca gatcattcat gacattcggc ggcggagaca aggaatttgt agtcgtgcag
       61 gagccggcgg aagccccggg taatcaattg gcggtgtcca tcgaagggga tccggtcgat
      121 ctgggcttca ctgttgcggc attgacgaat aactcgatcc gttgggagca ggacggagtg
      181 gcgttcttcg tagcatcttc cacattgaca cctgacgagc tgattgaagt cgcatcttcc
      241 atgcatccgg aagatagcaa ataagatgga attgtttgac gtgaaaagct cgtaggattg
      301 ataataggag catccagaga aaaacgaggg attcatatgg aacattatcg tcctacaaaa
      361 gcattcgtca atttatatgc gatacaagag aatctcaaaa gcttgaagca atatttgcct
      421 cgccatacat ccgtcatagc cgtcgtgaaa gcggacggct atgggcatgg agaggtcgaa
      481 gtggcacagg ctgcaattga tgcgggggcc cggatggtgt cggtagcgac gcctgacgag
      541 gccttacatt tgcgcaatgg gggcattgtc gccgatattc ttgtgatggg gccgtccccg
      601 gtggaatttg ccgagaaggc tgcagcacta ggaatccacg tcactgtctc ggatgccgga
      661 tggcttcgtc aagcggcccg gatggtggag gggaccagtc attcattgca cgtgcatgtg
      721 aaaatcgaca gcgggatggg caggatcggt cttcgggatg aaaatgccct tcaagagatt
      781 gtggaagtgg tcgaaggaac gtctgaaatc actcttgcag gcgccttcac gcatttcgcc
      841 tgcgcggatg aggaggattc cggcaaaacg gaagaccaat tcaatcggtt catggagttg
      901 gtgggcaaat tcccgaaaag gccgccgctc gtccatgcat cgaacagtgc agccacgttg
      961 ctttatccgg aatatgcact cgatgcggtc cgtttcggca tcagtctgta cggcatcgcc
     1021 ccttcccctt atgtagggac gaaattgcca ttcggactga agagagcttt cacactcgaa
     1081 acggaattgg cttttgtgaa aaagcttgaa aaaggcagtg cgatcagcta tgggggaaca
     1141 tacgaaacag tgcaggatga atggatcggc acattgccga tcggctatgc ggatggcttg
     1201 aaacgcgggc ttcgcggcca ggaggtgctg atcggcggcg aacggatgcc gattgtcggc
     1261 acgatttgca tggatcaatg catggtgaag ctgccgcgcg aatttggtgt gggggaaaaa
     1321 gtgacattga tcggcaggca aggagatcag gaaatcacga tggaggaatg ggcggaccgg
     1381 atcgggacga ttccatacga aatcgcggtc acaattggaa aacgcatacc gagaatatat
     1441 tgaacctcac atggataaga tgtatacagt tccaactttc gacaaatgac cgcttaattt
     1501 gaagattctt gtcaataaaa atccttcttt gtcttctcat tgtcgaagtt caatggtaag
     1561 atagacgttg aatagaatcg atcgagggaa cggggtctgt cggaggtgct agaattgcga
     1621 gagaaaaaaa taaaaatcgt aaaagtatcc aaagggaatg aaattgaaca tacggccgtc
     1681 catcaggagc cggagcgcgg agaattcgtt tatgtttcaa cgaagcggta tatgactgat
     1741 catgaagcgg ataccatccg tgaagcgatg atgatcggct acgttgaaat gtcgcaaatc
     1801 aacttacgaa tcgcaaagga atgcttgcat gcggaactcg aagcccagca tacggtggaa
     1861 cgtctcgtaa gcggaggatg aaaagttggc aataaaacgt ggggacgtct tttttgcaga
     1921 cctgtcaccc gtcgttggtt ccgaacaggg agggacaaga ccggtactgg tcatccagaa
     1981 cgacataggc aaccggttca gcccgacagt gatcgttgcg gcgatcactg cacaaatcca
     2041 gaaggctaaa ttgccgacac atgtcgaaat cgatgcggaa cggtatggat tcgagcggga
     2101 ttccgtgatc ctgctcgaac aggtccgcac gatcgacaag tcgaggctga cggataaaat
     2161 cacccatttg gatgaaccat tgatggagag ggttgacgaa gcgctcgaaa taagcttcgg
     2221 attagttaaa ttttgaatac atatgtaaaa gcactgcgga cctcgtcttc cggagtgttt
     2281 ttttattttc cggaaatttt agggacgtgg ctgtaaatag atgtccaatt cgactttttt
     2341 ctgtacaatg tacttatggg attttcaatt tggcgaggag gcatttgaac gcatgaataa
     2401 aatcatggta gaaggcatca atcagaacat ggatgaaatc atcgatcgtt ggataagaag
     2461 gatgaaggaa gaaaaagggg aacgtttttt ccacttcatg cctgatcatc ttgtcgaaaa
     2521 aacgagcagg gaattcgcag tgctaatgac ttccaacatt gtcgaatcat ccgcggtgaa
     2581 taccgaaaga attaacgagt tcactgaaaa aatcgtccgg ttcggttgga cgatcatatt
     2641 cgtgaataag gcgatcgata acttttcgga agttgtattc caatttcttg aagagaatgg
     2701 aatcataaag gaaagcaacc tgaggtcttt ccttctcaca ttcagaaatt ggatcaaccc
     2761 tcttcgagaa agtattatcg atgcctattc aacggaatgg gaaaggacag tcagcttgca
     2821 gaagatcgca ttgcaggagc tgtcggcttc tttaattccg gtattcgaca aaatttcggt
     2881 catgcctcta gtcggaacga tcgacacgga acgcgcgaag ttgatcatgg agaatttgct
     2941 tgagggcgtg gtaagccagc gggctgaagt tgtgctgctc gatataacag gcgtcccggt
     3001 cgtggatacg atggttgcac atcatatcat ccaggcggct gaagccgtgc gtcttgtcgg
     3061 cgcgaaatgc atgctcgtag gcatccgtcc tgaaatcgca cagacaattg tcacccttgg
     3121 catcaacctg catgaattca ccacgaaaag caccttgcaa aaggggatgc aagcagctct
     3181 cttaatgacg aaccgagcaa ttgtggaggt ggaagcaaaa tgaatgcacg cataccgatt
     3241 ttgaaattga atgaggttct catcgtatca atccaatggg agcttgatga ccagacggcc
     3301 attcaatttc aggaagatct attgaataaa atgcatgaaa ctgcggctct cggagttgta
     3361 atcgacttga caccgattga tttcattgat tccttcatcg caaaagtatt gggggatgtg
     3421 atcaatatga cagggttgat gggtgcgaaa gttgtcatca cgggcataca gccggcagtc
     3481 gccattacgt tgatcgagct cggcatccgt ttgagcaatg tcatgaccgc gctagacttg
     3541 gaaaacggtt tggataaact ttataaagaa ttggaggcct gacaatgaac tttaggtctt
     3601 ctgtagagat aatcacggaa tgggatatcg ttgctgcaag gcaattaggg cgtaacgaag
     3661 caaagaaggc cggtttcgga actgtcgacc aagcacgcat cacgaccgca atcagtgaat
     3721 tggcgaggaa tatctatctg tatgcaggaa aagggaagat cgagattgaa agagtttcgg
     3781 agggcgggct gttcggcatc aaaatcattg catcagatga aggtcccggg atccccgatc
     3841 ttcgcagagt gatggaagac ggcttttcga cttcgggcgg cctgggagcg ggaatgccag
     3901 gggttaagcg gctaatggat gaattcaaag tcgcttcgga gccgggggcc ggaacgatca
     3961 ttacgacaaa aaaatggctt cattgaggag gtgatggtgg atgccacaag aagtcggcag
     4021 gcagtataag gaaatcatga aaaaatactt ggagagccat agtgaagagg atctctatgt
     4081 cggacagcaa ttcagcaggc gattcattga aaaggaaata gcgcctgaag acgtcatcag
     4141 catacacaaa tctgcattat tcgaactgca ccctgagttg cggaacgaag tgtgggattc
     4201 actggacttc ctgatcgaaa tgatgatcca ctatggt
//
LOCUS       DASULI010000062         7075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_590117, whole
            genome shotgun sequence.
ACCESSION   DASULI010000062 DASULI010000000
VERSION     DASULI010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590117"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..862)
                     /locus_tag="VFX34_01395"
     CDS             complement(83..862)
                     /locus_tag="VFX34_01395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5563586.1"
                     /translation="MKKMSLTLLSIFLLLLLAACGQSEQKGQETATNGSSNSQSEDEA
                     QDEQSDETSSDDATNEEATTMYPQLSNEVAANEALVVMNTTMGPIKIKLFPEQAPKTV
                     ENFLTHAENGYYDGIIFHRVIKDFMIQGGDPTGTGMGGESIYGDSFEDEFTMDLFNIR
                     GALSMANAGPNTNGSQFFIVQASNPPASAAQLTQGGWPEEIAKAYEENGGTPHLDQKH
                     TVFGQVIEGMDIVDKIASAKTDRSDKPVEEISIESIEIIQK"
     gene            1079..2311
                     /locus_tag="VFX34_01400"
     CDS             1079..2311
                     /locus_tag="VFX34_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5563587.1"
                     /translation="MNVQKRNFIIIWFSNFLVAGTMTMIMPFLSLYIETFGDHSDAYV
                     QKWAGLIFGATFITALIMAPIWGRIADKYGFKPILLINGFGIAISVFMMGFVHSVEAF
                     FILRLFNGIVTGFIPTSLAFVSSQTSKEEAGKKLGTLQMGSVTGTLFGPALGGLMADA
                     FGFKYTFIVTSISVVIAALIVLFGIQEQKKVKDKKKVHYSRKVILSGLLHHRLMLNIM
                     LVTALIQIGNFSIQPLLSLYVAGLTDSNDVAFLAGLTFSAAGIGNLLFARKWGKLGDD
                     IGYEKVLGALLILSFIFIVPQAFVSTIWQLMIWRLLFGIAIGGMIPITTALVRREAPV
                     DMQGEVMGYNTSSRFLGNIIGPMFGGIVSGYIGISSVFILTGILFLIGFAFLYIAKRK
                     PVQDFEDFLAEENKQTNV"
     gene            2370..4226
                     /locus_tag="VFX34_01405"
     CDS             2370..4226
                     /locus_tag="VFX34_01405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5563588.1"
                     /translation="MKKFTSFLLIIVMIPLLFTIQRAVVKEWKEAQGLKAGIGETIEL
                     TEPSVSIPISMTDRNGKVFAEEYVEWRQPLPLDSIPAFIQQLFLVSEDQGFFEHKGYD
                     VAAIARAFAVNAMGDDMSQGASTITQQVVRMRFLTTEKTYERKFKELLYAAELEKQST
                     KNDILEMYLNEMFFGNQVYGIGAAATYYFNRPLDELTQAEMAFIAAIPNNPSKYDPLR
                     HFEHTKKRQELLLSVLSVNGAITEQEAESWKQEEIKLNVKTKANEFNMYSSYVLAELE
                     ELIGHSEGFTASIESATTPHERQAHVEALKKRTAEVIAGGIRIETALDPSKQKHDESS
                     LSSLLRMKDLQGGAVVIDNTTKEIVSIFGGKGFRKGDFNRAYQAVRQPGSAIKPLLVY
                     APYLESGPYKESTPIDSSNICIGSYCPTNIGGYRYGMTTVKEAFRHSHNTAAVRLFRK
                     VGIREAFEHLNVFRFKHITEEDFTYPAALGGFHRGVTPLELASAYSSFVDGMYSPPHA
                     IRTVKSRDGNTLYEWKNEPVKAWSATTVSTIRSLMEDVVFNGTGKGIRYSTSYTGAKT
                     GTTDRYKDLWVAGMNDRYTTAVWIGYDKPASIQRQSNQKIHLRAFNELLSMP"
     gene            complement(4271..4831)
                     /locus_tag="VFX34_01410"
     CDS             complement(4271..4831)
                     /locus_tag="VFX34_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5366 family protein"
                     /protein_id="HEX5563589.1"
                     /translation="MRNPYLFGYLPFLTITLFSLTFGVYLVGASIVFFGQIGLYAGMQ
                     EFLTDTQLRLFLLVIYSLAFFMIFSALKLIAETIHETAMLFFSIDAVGESYSEAKSGN
                     LIYFFGALASVSGIQSLKVLLAIFVLTTFVYFVFTMFKLSKFMTMPSMVGLLFFEILV
                     WGLFLSGIIYIVLKLYNGVLASLPVV"
     gene            4994..>7075
                     /locus_tag="VFX34_01415"
     CDS             4994..>7075
                     /locus_tag="VFX34_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HEX5563590.1"
                     /translation="MEFVLLIFLPIVAALFIPLLFRKMKGIHTGWFVLIVPVLLFIYY
                     TSFIKTTMDGGHALSELQWIPSLGISFVSYLDGLSILFSLLITGIGALVVLYSIFYLD
                     KNKEKLGNFYVYLLLFMSAMLGVVQSDNVISLYLFWELTSISSFLLIGYWFTRDGSRF
                     GALKSMVVTVFGGLMLLGGFILLGIMGGTFSIRELIAGSSSFAGHEFFTLAIVLILLG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLVARFTPIFAVSEVWIWLV
                     TGIGLLTLFWGSLFAVKQTDLKAILAFSTVSQLGLIMSLLGAGAITFHVNDTLFSFAM
                     FAAVFHLINHATFKGSLFMIAGIVDHETGTRDIRKLGGLMSIMPVSFTVAFIGSMSMA
                     GLPPFNGFLSKEMFLQSMLAIRHFELFNFATWGVVFPVVAWIASVFTFIYSFYFVFRT
                     FTGKRKAAKLPLEPHEAPIGMLISPVILAALVIGIFFIPNVIGKWLVKPAVMAIQPYQ
                     YSHPSEVAVQVSAWHGFASVELWMTVGIVLIGSLLYLSLSKWQKFYGIQPDNVSLNGL
                     YDATMTFSEKGMNRLSRLYMTGLIRTYLLYIFAFLSFTTIAMLFLQDAFVVDMSSFSK
                     LSAYGLINAVILLLAVSFVLLSKTRLGAIISLGAVGYSVALFFVIFKAPDLALTQLVI
                     ETVSVALFLLAF"
BASE COUNT         1835 a         1606 c         1631 g         2003 t
ORIGIN      
        1 tgctgggata tactctgatt tatcttcggg aaagtaaagg aacggcatta gtagaacgtt
       61 catttacgta cacttccttc tattatttct ggatgatttc aatcgattca atcgaaattt
      121 cttctaccgg cttatctgat cgatctgtct tggcagatgc gattttatct acgatatcca
      181 tgccttcgat cacttgtccg aaaacggtat gcttttgatc gaggtgcgga gttccgccgt
      241 tctcttcgta tgcttttgca atttcttcag gccagccgcc ttgtgtcaat tgagctgccg
      301 atgcaggtgg atttgaagct tgcacgatga aaaattggct gccgttcgtg ttcgggcctg
      361 cattcgccat cgacaatgcc cctcgaatat tgaaaagatc catcgtgaat tcatcttcga
      421 atgaatcacc gtagatgctt tccccaccca tgcctgttcc tgtcgggtct ccgccttgga
      481 tcatgaaatc cttgatgaca cggtggaaaa tgattccgtc atagtagccg ttctctgcat
      541 gtgtcaagaa gttctccaca gtttttggcg cttgttccgg gaatagtttg attttaatcg
      601 gacccattgt cgtattcatg acgacaagag cttcgttcgc tgcaacttca ttcgataatt
      661 gtggatacat cgtcgtggcc tcctcattgg tcgcatcatc tgatgatgtc tcatcagact
      721 gttcatcttg tgcttcgtct tcgctttgcg agtttgacga tccgtttgtt gctgtttcct
      781 gccccttttg ctcactttga ccgcaagcgg caagcagcaa taatagaaag attgaaagca
      841 atgttagcga catctttttc ataatcattc accctgaaac agtttaccat aaccttagaa
      901 ttttttcgat ttataagcat gaaatttcct tcggagctcg aaatgttcta taattgataa
      961 atcttagaaa cgtttacttg cattaagcaa agggaatctc aagtctgata tgcctttttt
     1021 caattaaaac agcgccgctt atgaatagcg gcgggtcaca tgaaagcggg tcacttacat
     1081 gaatgtacaa aaacggaatt tcatcatcat ttggttctca aactttctag ttgccggaac
     1141 gatgacgatg atcatgccat tcctttcttt atatatcgaa acgttcgggg atcattcgga
     1201 tgcatacgtc cagaagtggg caggattgat tttcggagcg acgttcatta cagctttgat
     1261 catggcacca atatggggtc gcatcgccga taaatatggg tttaaaccga tattgctcat
     1321 taacggcttc ggaattgcta tatccgtttt tatgatgggg ttcgtccatt ccgttgaagc
     1381 attcttcatc ctcaggcttt tcaacgggat tgtcacagga ttcattccga cttcgctcgc
     1441 atttgtttcc tcccaaacgt caaaagaaga agccggcaaa aagcttggga cattgcaaat
     1501 gggaagtgtg accggaacgc ttttcggacc agcattaggc ggattaatgg ccgatgcgtt
     1561 cgggtttaaa tatacattca tcgtcacttc gatatcagtc gtcattgcag ctctgatcgt
     1621 gttattcgga attcaggaac aaaagaaagt gaaagataag aagaaggtcc actactcgcg
     1681 gaaagtaatc cttagcggac tgctccatca tcgtctaatg ttgaatatta tgcttgtcac
     1741 tgccttgatc caaatcggga acttcagcat ccaacccctc ctttcacttt acgtggcagg
     1801 actgaccgat tcgaatgatg tcgcattcct cgctggactc actttcagcg ccgcaggaat
     1861 cggaaacctt ctgttcgcta ggaaatgggg gaaactgggc gatgacatcg ggtatgaaaa
     1921 agtgctcggc gcattgctga tcctttcctt catcttcatc gtcccgcaag catttgtttc
     1981 taccatctgg caattgatga tttggcggct cctgttcggg attgcaatag gcggcatgat
     2041 tccgatcacg actgctcttg taaggcgtga agcaccggtc gatatgcaag gggaagtgat
     2101 gggatacaat acgagttccc gcttccttgg caatatcatc ggaccgatgt ttggtggaat
     2161 cgtcagtggt tacattggta tttcgtccgt gttcatttta acgggcatcc ttttcctaat
     2221 agggttcgca ttcctttata ttgcaaaacg aaagcccgta caggattttg aagatttcct
     2281 ggcggaggag aacaaacaaa caaacgtgta attttagcac atttccaaaa cggaaatgtg
     2341 ctattatttt tgcataggga ggggactttt tgaaaaagtt tacaagtttt ttattaatta
     2401 tcgtgatgat tcccctgctt ttcaccatcc aacgagccgt agtgaaagag tggaaagaag
     2461 cgcaaggctt gaaggcggga atcggcgaaa cgattgaact taccgaaccg tcggtcagca
     2521 ttccaatttc gatgaccgat cggaatggaa aggtatttgc cgaggagtat gtagaatgga
     2581 gacagccgct tcctttggat tccatccctg catttataca gcaacttttc cttgtgagtg
     2641 aagaccaagg tttttttgaa cataaaggct atgatgtagc ggcaattgcc cgtgctttcg
     2701 ccgtcaacgc gatgggcgac gacatgagcc aaggtgcttc caccatcacc cagcaggtag
     2761 tgaggatgcg attcctgacg accgaaaaga cgtatgaacg taaattcaaa gaattgcttt
     2821 acgcagcaga actggaaaaa cagtcgacaa aaaacgatat tcttgaaatg tatttgaatg
     2881 aaatgttttt cggcaaccaa gtgtatggca tcggagcggc cgccacttat tatttcaaca
     2941 ggccgcttga tgaattaacg caagctgaaa tggcgttcat tgccgcaatc ccgaacaatc
     3001 cttccaagta tgacccgctc cgtcatttcg aacatactaa aaagaggcag gagcttctct
     3061 tatctgtcct ttcagtaaac ggcgccatta cggagcagga agctgaatct tggaagcagg
     3121 aagaaataaa gttgaatgtg aaaacgaagg cgaatgaatt caacatgtat agctcgtatg
     3181 tcttagcgga gctggaagag ttgatcggcc attctgaagg attcacagcg tcgattgaat
     3241 ctgcaaccac tccacatgaa agacaagcgc atgttgaggc actgaaaaaa aggaccgccg
     3301 aagtcattgc cggaggcatc cggattgaaa cggcacttga tccgtcaaag caaaagcacg
     3361 acgagtcttc cctttcttcc ctcttaagaa tgaaggactt gcagggcgga gctgttgtca
     3421 ttgataatac gacaaaagaa atcgtgagca ttttcggggg aaaggggttt cgcaaagggg
     3481 acttcaacag ggcctatcag gctgtaaggc aacccggctc agcgattaag cctttgcttg
     3541 tctatgcccc gtatcttgag agcgggcctt ataaagaaag cacgccgatc gacagcagca
     3601 atatttgcat cggttcgtac tgtccgacaa atatcggagg gtataggtat gggatgacga
     3661 ctgtgaagga agcgttccgt catagccata acacggcggc agtgcggttg ttccgcaagg
     3721 tggggattag agaggctttt gagcacttga acgttttccg tttcaaacat ataacggaag
     3781 aggacttcac ctaccccgca gccctcggag gtttccatcg gggcgtgacc ccactcgagc
     3841 tcgcctccgc gtattccagt ttcgtcgatg gcatgtattc cccgccgcac gcaatcagga
     3901 ctgttaaaag ccgtgacggg aatacgctct atgaatggaa gaacgagccg gtgaaagcct
     3961 ggtctgcaac aaccgtctct acaatccgga gtttgatgga agatgtcgtc tttaatggta
     4021 caggtaaagg cattcgatat tcgacatcgt atactggggc aaaaacagga acgacagacc
     4081 gttacaaaga cttatgggtc gcgggaatga acgaccgata cacaacagct gtctggatcg
     4141 gctacgacaa acccgcctcc atccagcgcc aaagcaatca aaagatacat ttgcgggcat
     4201 tcaatgaatt gctctccatg ccgtaaaatg aaaaccgttg aaatggccat aaggcaattc
     4261 aacggtttct tcataccacc gggaggctag caagaacccc gttataaagc ttcaacacaa
     4321 tgtaaataat accggataag aagaggcccc aaacgaggat ttcaaaaaac aacaagccga
     4381 ccatgcttgg cattgtcatg aacttgctca atttgaacat cgtaaataca aaatagacaa
     4441 aagtcgtcaa cacgaagatc gccaacagaa ccttcagcga ttggatccca ctgacagaag
     4501 cgagcgcacc gaaaaaatag atgagattgc cgcttttcgc ctcactatac gactccccta
     4561 ccgcatcgat cgaaaagaac agcatcgccg tttcatgaat cgtttccgca atcaatttca
     4621 aagccgaaaa aatcatgaag aaagcaagcg aataaataac aagaagaaaa aggcgcagct
     4681 gcgtatccgt aaggaactct tgcatgcctg cataaagacc gatttgaccg aagaatacga
     4741 tcgaagcccc caccaaatag accccgaacg tcaaactgaa aagtgtaatt gtcaaaaatg
     4801 gcaaatagcc aaaaagataa ggattcctca tttaacaccc tcactgcaaa tagtttatct
     4861 ttccctataa tataagaagg gaaacatatt tcgcaacaat gtcaaaatgt gtcctatgca
     4921 atccctcgcc atttaagtta tactttaaaa caatgtaaat aataatgttt cactcattgg
     4981 aaggaggatt actttggaat tcgtattact gattttcctg ccgattgtgg cggccctatt
     5041 cataccgctc cttttcagaa agatgaaagg tatacatact ggttggttcg tcttgatcgt
     5101 ccccgttctt ttattcattt attatacttc gttcatcaaa acgacaatgg acggcggaca
     5161 tgccttatct gaactgcaat ggatcccctc actcggcatc tcattcgtat cctatttaga
     5221 tggcttgagt atattattct ccctgcttat tacggggatt ggggcgctcg ttgtactgta
     5281 ttcgatcttt taccttgata agaacaagga gaagctaggc aacttctacg tctatcttct
     5341 tctttttatg agcgcaatgc tcggggttgt ccaatcggat aatgtcatct ctctctatct
     5401 gttttgggag ctgacatcca tttcttcatt tttactgatc ggttattggt tcacccggga
     5461 cggttccaga ttcggtgcgt tgaaatcgat ggtggtaact gtattcggcg gactgatgtt
     5521 gctaggggga ttcatccttc tgggcattat gggaggcacg ttctccatcc gtgaattgat
     5581 tgccggatcc tcgtcatttg ctggccatga atttttcacg ctggctatcg tcctcatatt
     5641 gctgggggca ttcacgaaat ccgcgcagtt tcctttttac atctggttgc cggatgcaat
     5701 ggaagcgcct acacccgtca gtgcttattt gcactcggcg acgatggtta aggcaggctt
     5761 gtatcttgtc gcccgtttca cacctatttt tgcagtatca gaagtgtgga tctggctcgt
     5821 cacgggtatt gggctgctta cgctgttctg gggctcactc tttgcagtga aacagaccga
     5881 tctgaaagcg attctcgcat tctcgaccgt cagccagctc ggcttgatta tgtcgttgct
     5941 tggcgcaggc gctatcacat tccatgtgaa tgatacgttg ttctcttttg caatgttcgc
     6001 tgccgttttc catttgatca accacgcgac attcaaagga agtttgttca tgattgccgg
     6061 gattgtcgat catgaaacag ggacacggga tatccgtaaa cttggtggcc tcatgagcat
     6121 catgcctgtc agcttcaccg tcgcgttcat cggatcgatg tcgatggcag ggttgccgcc
     6181 attcaatgga tttctgagta aagagatgtt tttgcaatcg atgcttgcaa tccgccactt
     6241 cgaattgttc aattttgcga catggggagt tgttttccct gtagtggcat ggattgctag
     6301 tgtattcacg ttcatttata gcttttactt tgtgttcagg acatttaccg gaaagaggaa
     6361 agccgcgaag ttgccgcttg agcctcatga agcaccgatc gggatgctca tatcccccgt
     6421 catccttgca gcacttgtca taggcatctt cttcataccg aatgtaatcg ggaaatggct
     6481 cgtcaagcct gccgttatgg caatccagcc gtatcagtat tcccatcctt cggaagttgc
     6541 tgtgcaagtt tccgcatggc acggtttcgc ctccgtcgaa ctatggatga cggtcggaat
     6601 cgtacttatc ggctcacttc tatacttgtc gctttcgaaa tggcagaagt tttatggaat
     6661 ccagcctgac aacgtttcct tgaatggatt gtatgacgca acaatgacgt tcagtgaaaa
     6721 agggatgaat cgactatccc gcctttatat gacggggctc atccgtacgt acctgttgta
     6781 catatttgca tttttgtcgt ttacgacgat tgcgatgtta ttccttcaag atgcatttgt
     6841 tgtggatatg tccagctttt caaaattaag tgcgtacggt ttgatcaatg cagtcattct
     6901 tctgcttgca gtcagttttg tattactgtc caaaacgaga ctcggggcga ttatttcgct
     6961 tggagcagtc ggatactcgg tcgcactgtt tttcgtcatt ttcaaagcgc cggatttggc
     7021 tcttacgcaa ttggtcattg aaacggtatc agttgcgctg ttcctattgg cattc
//
LOCUS       DASULI010000063         4818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_629108, whole
            genome shotgun sequence.
ACCESSION   DASULI010000063 DASULI010000000
VERSION     DASULI010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629108"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VFX34_01420"
     CDS             complement(<1..1248)
                     /locus_tag="VFX34_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEX5563591.1"
                     /translation="MFRDMYFANMLSWLPELILGLVVLLIGFFIAKAVENAVYRRLKK
                     SRVNERLDVKEGKWNVEKIISKVFFFGILLLAFLLFFNIMNASMIATPFMTMYSGIGG
                     AVLGILKAGLILLLAWVLALIVKKVILAVGGKMKLNKYVAKVGGSPDEIDKSKWVETA
                     ANIAFYVVLLLFVPAVLHALGLSGISGPFEGMLESFMNFIPKLVGAALIFAVGWVVAK
                     IVRTIVTKLLESVGVDRFSHKLKSSSRGTSISGIIGTIVFVLIMLPVTVSALEVLDLE
                     GISQPAIAMLNDIMTMLPKIAVAIVLVLVGIYLAKWVKRIVVSLLDNLGVNSIFGKIG
                     VRSNASMPTFSQIIGTIVQVVIILLFVVEALQILNLNFMVTLATGIFAYLPMVVAAVV
                     ILAIGFWLANLAEKFVGSIMTTQS"
     gene            complement(1404..4490)
                     /locus_tag="VFX34_01425"
     CDS             complement(1404..4490)
                     /locus_tag="VFX34_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5563592.1"
                     /translation="MKGLVNFVLKNKLAVWLLTIIITVSGVYSATQMKTETLPDISIP
                     YLMVMGVYPGATPEQVMEEVSVPIERAVENLDHVKSVYSNSYSNMSSIQVEYDYGIDM
                     DEAKRELASAMDSLTLPEGAQEPVITAISMNMMPVVALSVSSTTEDIVELTSTVEDIL
                     LPKIEKIDGVASATISGQHIEEVELTYDEEKMAQLGLTEDSVKQMIQASNMEVSLGLF
                     EFEEGEQAVAVDGKFMTIDELKGMLIPVTPSQANPSPFVKLGDIAMIEKVGKVQSVSR
                     TNGENAIAIQIVKGQRANTVDVVNDVKKLMKDEEKAIKGLVVDVSLDQGEPIEKSVST
                     MVEKALFGGLIAILIILLFLRDFKSTIISIVSIPVSVFMALLLLNWMDITLNIMTLGA
                     ITVAIGRVIDDSIVVVENIYRRLHLKTEKLSGRALIREATIEMFKPIMSSTLVTVAVF
                     APLIFVGGMVGELFMPFALTMSFALGASLLVAITIVPALSHFLFKKKLYGEKSESQHK
                     EAGKMANGYKRILGWSLNHKVITSIISVVLLAGSLGLMPLIGFSFLGSDEEKVMYITY
                     TPATGELQDETLANVEVVEKEMLKRDDIDIVQLSIMDSADPMTAMMGGGSNGALMYLI
                     FDPDMKDFQGARANVEDYVFNIGQSGEWKSQDFGSMGMSTNEISYTFYSEDLSSLKEA
                     VLQVEKIMGDNKGIEDVSSSAEDAYVQYTLKATQDELLQYGLTTGQIIMMLNPSRTQE
                     VVTTVGKDRDELEVVVKQKATEQPKSIDEMLEQEIPTALGTTMKLSDLVKVEEGSTFN
                     TLARSKGQYYATVSGKLVDADISKATSEVDKAIAELDLPKGVAVDTAGVAADMEEAFT
                     QLGIAMLAAVAIVYFILVVTFREGVAPFAILFSLPFAVIGSFVGLLIAGETISVPVMM
                     GLLMLIGIVVTNAIVLVDRIIRMERTGLQRREAVLEAGATRLRPILMTAIATIGALIP
                     LALGNGAGGGLISKGLGITVIGGLISSTLLTLIVVPIVYEILSKIFKKNRLDLEED"
     gene            4590..>4818
                     /locus_tag="VFX34_01430"
     CDS             4590..>4818
                     /locus_tag="VFX34_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5563593.1"
                     /translation="MTKKQLIMEKALELFAKQGFEATSVQQITDHCSISKGAFYLSFK
                     SKDELIVALIDHFMRQFTADIDYVVNHKQNDG"
BASE COUNT         1317 a         1261 c          993 g         1247 t
ORIGIN      
        1 tgattgcgtc gtcatgatac tgccgacgaa tttttcggca aggttcgcga gccagaagcc
       61 aatcgccaag atgacgaccg ctgcgacgac catcggcaag tacgcgaaaa tacctgtcgc
      121 gagtgtaacc atgaagttca ggttaaggat ttgaagcgct tcaacgacga acagcaaaat
      181 gataacgact tggacaatcg ttccgataat ttgggagaac gtcggcatag aagcattcga
      241 acgtacgccg atttttccga aaatcgaatt gacgcctaga ttatccaata aggacacgac
      301 aatccgcttg acccatttcg ccaagtagat accaaccaat acaaggacaa tagcaaccgc
      361 aattttcggc agcatcgtca taatgtcgtt cagcatagca atggcaggtt gggaaatccc
      421 ttcaaggtcc aatacttcca atgcagaaac cgtcaccggc agcatgatga ggacaaaaac
      481 gatcgtgccg atgatgcctg aaatgcttgt cccacgtgaa gaggatttca gcttatgcga
      541 aaaccggtca acgccgaccg attcaagcag cttcgtaaca attgttcgga cgattttagc
      601 gacaacccat ccaaccgcga aaatcaatgc cgctccgacg agtttcggaa tgaagttcat
      661 gaagctttcc aacattcctt cgaacggtcc gctaattccg ctcagaccga gcgcatgaag
      721 gacggccggc acaaatagca gtaagacgac atagaacgca atattcgcag ccgtctcgac
      781 ccatttcgac ttgtcgattt cgtcagggga gccccctact ttcgcaacgt acttattgag
      841 cttcatcttg ccgccgaccg ccaaaatcac cttcttgacg atcaatgcaa gaacccaagc
      901 caacagcagg atgagccccg ctttcaagat gccgagcacc gccccgccga ttcctgaata
      961 catcgtcatg aatggcgttg caatcattga cgcgttcatg atgttaaaga agagaaggaa
     1021 tgctaataga aggattccga agaagaagac tttgctgatg atcttttcca cattccattt
     1081 tccttcttta acgtccagcc tttcattgac ccttgatttc ttcaaacgtc tgtacaccgc
     1141 attttcgact gctttcgcaa taaagaagcc gatcagcaag acgactaatc cgagaatcag
     1201 ttcgggcagc cagcttagca tattggcaaa atacatatct ctgaacattc cgtgtcctcc
     1261 attcatttag gtagtctagt taagactatt cctataaatg tggaggtcta aacttattaa
     1321 tctagtaatt atggaagaga aagcaagtaa acatcaaaaa gacagaagaa ggaacaagtt
     1381 tctgctcctt cttctgcact taattaatcc tcttccaaat ccaaacggtt tttcttgaag
     1441 attttcgata agatttcgta cacgatcgga acgacgatca gcgtcaacaa tgtggagctg
     1501 atcaaaccgc cgataaccgt gatgccaaga cctttcgaaa tcagtccgcc gcctgctcca
     1561 ttacctagtg ccaatggaat gagggcgccg attgtcgcga tcgctgtcat caagattgga
     1621 cgcagacgtg tagcgcccgc ttccaacaca gcttcacgcc tttgaagacc tgtacgttcc
     1681 atccgaataa tgcggtccac gagtacaatc gcattcgtga caacgatacc gattaacatc
     1741 aatagaccca tcatgactgg aacggaaatc gtttcgcctg cgatgagcaa gccgacgaac
     1801 gaaccgatca ctgcaaatgg cagcgagaaa aggatcgcga acggcgcgac accttcacgg
     1861 aacgtcacaa cgaggatgaa atagacaatc gctactgccg caagcatcgc aatgccgagc
     1921 tgcgtaaatg cttcttccat gtcagccgcg acacctgccg tatcgacagc aacacctttc
     1981 ggcaaatcca attcggcaat tgccttgtca acttcagatg ttgcttttga aatgtctgcg
     2041 tctactagct taccggaaac agtcgcataa tattgtcctt tgcttcgggc cagcgtattg
     2101 aatgtagatc cttcttcgac tttcactaag tcggaaagct tcattgtcgt ccctaacgca
     2161 gtcggaattt cctgttccag catttcatcg atcgatttcg gctgttcagt cgccttctgc
     2221 ttcacgacaa cttccaattc atcgcgatct ttcccgaccg tcgtgacaac ttcctgcgtc
     2281 cgcgacgggt taagcatcat gatgatctgc ccggtcgtca atccatactg caacagttca
     2341 tcctgcgttg ctttcaatgt gtactgaacg tatgcatcct cagcacttga agagacatcc
     2401 tcaatgcctt tgttatctcc catgattttt tcaacctgca ggacagcttc cttcaggctg
     2461 cttaaatctt cactatagaa cgtgtagctg atttcatttg tggacatccc catcgaaccg
     2521 aaatcctggc tcttccattc tccggactga ccaatgttga atacatagtc ttcaacattg
     2581 gcacgggctc cttggaagtc tttcatatcc gggtcaaaga taagatacat aagcgcacca
     2641 ttcgagccgc cgcccatcat cgcggtcatc gggtcggcgc tgtccatgat ggataattga
     2701 acgatgtcaa tatcgtcgcg tttcagcatt tccttctcga caacttcgac atttgccaac
     2761 gtttcatcct gaagctcacc cgtcgccggc gtgtacgtaa tgtacatcac tttttcttca
     2821 tcgctgccta agaaactgaa accgatcagc ggcataagcc ctaggctgcc cgccaataga
     2881 acgacggaaa tgatcgatgt aatgacttta tgattcagcg accacccaag gatacgctta
     2941 tacccattcg ccattttgcc ggcttcttta tgctggcttt ccgacttttc accatacagt
     3001 tttttcttga ataaaaaatg ggacaatgct gggacgattg taatcgcaac gagcaatgaa
     3061 gcacccagcg caaacgacat cgtcaatgcg aacggcatga acagctctcc gaccataccg
     3121 cctacaaaga tcagcggcgc aaataccgca actgtgacga gcgtcgatga catgatcggc
     3181 ttgaacatct cgattgtcgc ttcgcgaatc aatgcacggc ctgataattt ctccgttttc
     3241 aaatgaagtc gccggtatat gttttccacg acgacgatgg agtcatcgat gacccggccg
     3301 atggcgacag taatcgcacc aagcgtcata atattcagcg ttatatccat ccagttcagc
     3361 agcaataacg ccataaatac tgaaaccgga attgaaacaa tggagatgat tgtagatttg
     3421 aaatctcgca agaagagtag gatgatcaat attgcaatca aaccaccgaa aagcgccttt
     3481 tcaaccatcg tcgataccga tttctcgatc ggttctccct ggtcaagcga tacatcgaca
     3541 actaatcctt tgatcgcttt ttcttcatct ttcattaact tcttcacgtc atttacgaca
     3601 tcgactgtgt tagcccgctg tcctttgacg atttggatcg caatcgcatt ttccccattt
     3661 gtacgggaaa cagattgcac cttgccaact ttttcgatca tcgcaatatc accgagcttc
     3721 acgaatggcg atggattcgc ctgtgatggc gtgacaggaa tgagcatgcc cttcagttca
     3781 tctatcgtca tgaacttgcc gtcgacagcg accgcttgct caccttcctc aaattcaaag
     3841 aggcctagtg aaacttccat attgctcgct tggatcattt gctttacgga gtcttccgtc
     3901 aaaccaagct gagccatttt ttcctcgtcg tacgtaagct ccacttcttc aatatgctgc
     3961 cccgaaatcg tcgcagatgc aacgccatcg atcttttcga ttttcggaag caaaatgtct
     4021 tcgacagtgg atgtcagttc tacaatatcc tccgttgtac tgctcacgct tagcgcaacg
     4081 acaggcatca tattcatgct gatcgcagtg atgaccggtt cctgcgcacc ttccggcaat
     4141 gtcaacgaat ccatcgcgga cgccaattcc cgcttcgctt cgtccatatc aatgccgtaa
     4201 tcgtattcca cttgaatgct ggacatattg gaataggaat tggagtagac ggatttcaca
     4261 tgatccaagt tctctaccgc cctctcgata ggtaccgaga cttcttccat gacctgctcc
     4321 ggagttgcac cgggatagac tcccatgacc attaagtacg gaattgatat atccggcagc
     4381 gtctccgttt tcatttgcgt tgccgaataa acccccgaca cggttataat aatggtgagc
     4441 aaccatactg ctaatttatt ctttagaaca aaattgacta accctttcac ccttacacct
     4501 tcccctacat tgacagtttt gactcagata tttatactaa tgactatgcg gtcaactgtc
     4561 aagaaataac ataggagcga tttgaatata tgacaaaaaa acaactaatt atggaaaagg
     4621 ctttagagct ttttgcaaaa caaggatttg aagcgacgtc tgtccagcaa ataactgatc
     4681 attgcagcat ttcaaaaggc gccttctatt tatcgttcaa gtcgaaagac gagttgatcg
     4741 tagcattgat tgatcacttc atgagacagt tcaccgcaga tattgactat gtggtcaacc
     4801 acaaacagaa tgacggtg
//
LOCUS       DASULI010000064         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_416649, whole
            genome shotgun sequence.
ACCESSION   DASULI010000064 DASULI010000000
VERSION     DASULI010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416649"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFX34_01435"
     CDS             <1..431
                     /locus_tag="VFX34_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019419978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX5563594.1"
                     /translation="FISLIYLINLITGAIGGVFGFESLTLEKILGYLFLPIAFLIGVP
                     WEEALQAGSFIGQKFILNEFVAYTSFASEIANLSPKSVAMISFALCGFANLSALAMLI
                     GGLGGMAPSRRQDITEMGFRAIIAATLANLTSAAIAGMLI"
     gene            559..855
                     /locus_tag="VFX34_01440"
     CDS             559..855
                     /locus_tag="VFX34_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5563595.1"
                     /translation="MKEITTAELQKKLENGETLHLIDVREDDEVAAGMIPGAVHIPLG
                     EVPEHLDELDKTTPYVIICRSGGRSGRAAEFLEAQGYDVTNMVGGMLDWQGNVE"
     gene            complement(930..1259)
                     /locus_tag="VFX34_01445"
     CDS             complement(930..1259)
                     /locus_tag="VFX34_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563596.1"
                     /translation="MGETNGLSGKSANTDVLDSPLNNYPAFLLPFQAVSTCYQALVTG
                     FQAVLMLHQAVSLLHQADATSIRRFNGARKVLLEFEWKLLEFQQILLEFDIVMAQSPT
                     VMLTSRL"
     gene            1337..>2039
                     /locus_tag="VFX34_01450"
     CDS             1337..>2039
                     /locus_tag="VFX34_01450"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5563597.1"
                     /translation="MKRWLIIGLLFASSLLVVLYGVNASDRGFFMPEQLGGLKIVIDP
                     GHGGLDGGASSGDVVERDITLSISHELKKRLEKKGAMVVMTREKEGDAIAEHAPSDTF
                     GTNRERKFADLKLRESITIEEDPDLFISVHVNAIPESRWRGAQVFYHAEGAEGGEMLA
                     KSIQNSFKERLKNTDREALSIKGVYLLKKVPMPAVLVETGFISNPEEKALLTDPAYHG
                     KVADAIVDGIVQYHLS"
BASE COUNT          530 a          426 c          567 g          516 t
ORIGIN      
        1 cgtttatctc tctcatttat ttgatcaacc taatcactgg agcgattggt ggggtcttcg
       61 gatttgaatc gttaacgctt gaaaaaatat tagggtacct attcctaccg attgctttcc
      121 tcatcggagt tccttgggaa gaagcattgc aagccggttc attcatcggg caaaaattca
      181 ttctgaacga attcgttgcc tacacttctt ttgcatctga aatagccaac ctgtcaccta
      241 agagcgtcgc tatgatcagc tttgcgctat gcggcttcgc caatctttca gcgctcgcca
      301 tgcttattgg cggcttgggg gggatggctc cttcgagaag acaagacatt acggagatgg
      361 gattccgagc aatcatcgcg gcgacacttg ccaatctgac gagtgcggcc attgctggga
      421 tgctgattta ataaaggttg cttctatata tgaagggatt gccagctttt ggggcaatcc
      481 ttttttgcaa tcaaagtgct ggactgcatg cctcgctcct atataagatg gaaggagtga
      541 atgcgaggag gatttgacat gaaagagata acgacagcag aattacagaa gaagcttgaa
      601 aatggcgaaa cccttcattt gattgatgta agggaagacg atgaagttgc ggcggggatg
      661 attccgggcg cggtccatat tccgttggga gaagttccgg aacaccttga tgagcttgat
      721 aaaacaacgc cgtatgtgat catttgccgt tcaggcggcc gcagcggacg tgcagcagag
      781 ttcctggagg cgcagggata tgacgtgacg aatatggtcg gcggcatgtt ggactggcaa
      841 gggaatgttg aataagtaat gtgacaaggg ctgtccagaa cgtcaaaatg actttctgga
      901 cagcccttgt gtgtattaac gtgatgggtc taaagtcgcg aggtgagcat aactgtagga
      961 gattgcgcca taactatgtc aaactcgagc aaaatctgct ggaactcgag caatttccat
     1021 tcaaactcga gcaaaacttt tcgcgcgcca ttgaagcgcc taatacttgt ggcatccgcc
     1081 tgatgcaaca atgaaaccgc ctgatgcaac atcaggaccg cctgaaaacc tgtcaccaac
     1141 gcctgatagc acgtcgagac cgcctgaaac ggcagcagaa acgccggata attattcagc
     1201 gggctatcca gaacgtcggt attcgctgac tttccggaca gcccatttgt ttcacccaag
     1261 gacttcaaat atgtgataca agcattcata tcttggccaa ggtgtgcgta tagtggagga
     1321 aaaaggcggt gtgagattga aacggtggct tatcatcggg ttgttatttg cgagctcact
     1381 tcttgttgtg ctctacggtg tgaatgcctc ggacagaggg tttttcatgc ctgaacagct
     1441 tgggggcttg aagatagtca ttgaccccgg gcatggtgga ttggatggag gagcctcgtc
     1501 gggcgatgtg gtggaaaggg atatcacttt gagcatttcc catgagttga aaaagcggct
     1561 tgagaaaaaa ggtgcaatgg ttgtcatgac acgtgagaag gagggcgatg cgattgcgga
     1621 gcatgcgccg agtgacacgt ttggaacgaa ccgtgagcgc aaatttgccg atttaaagct
     1681 acgggaaagc atcacgattg aagaggatcc ggatttgttc atcagtgtcc acgtcaatgc
     1741 aattcctgag tccagatgga gaggggcaca agtgttttac catgcggaag gcgctgaggg
     1801 cggtgaaatg ttagcgaagt cgattcaaaa ttcattcaag gaaaggttaa agaatacgga
     1861 tcgcgaagca ttgtccatca agggcgttta tttgctgaaa aaggttccga tgcctgccgt
     1921 tctcgttgaa acgggtttca tttccaatcc tgaggaaaag gcattgttga cggaccctgc
     1981 ctatcatggc aaagtggcgg atgctatcgt ggatgggatc gtccaatatc atttgtccg
//
LOCUS       DASULI010000065         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1374450,
            whole genome shotgun sequence.
ACCESSION   DASULI010000065 DASULI010000000
VERSION     DASULI010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374450"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..940
                     /gene="fabD"
                     /locus_tag="VFX34_01455"
     CDS             5..940
                     /gene="fabD"
                     /locus_tag="VFX34_01455"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019416050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5563598.1"
                     /translation="MTKVAFIFPGQGSQSVGMGSELFNGGGKYLERADEILQFGLSRL
                     ITEGPQEELTLTYNAQPALLTVGAMIASRLIEEGIKPDYTAGHSLGEYTALVASGVLS
                     FEDGVSVVHKRGLFMNDAVPAGEGAMAAILGLDGEKLQEVTDSITAEGNPVQPANLNC
                     PGQIVISGTKAGVEKACVELKEAGAKRAIPLDVSGPFHSSLMKPAAERLKNALDEVEM
                     KDASIPVVANVNAETVSDSEEIKQLLVEQLYSPVRWEDSVRELINLGVTHFIECGPGK
                     VLSGLVKKIDRSVTVLPVYDEETLQTVIDAAKGWE"
     gene            940..1686
                     /gene="fabG"
                     /locus_tag="VFX34_01460"
     CDS             940..1686
                     /gene="fabG"
                     /locus_tag="VFX34_01460"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX5563599.1"
                     /translation="MGRFEGKTAIVTGASRGIGREIAILLGKEGARVAVNYSGSKEKA
                     EEVVELIKQSGGDAFAIQANVSDAEQVKQMIDETLKTFGSIDMLVNNAGITRDNLLMR
                     MKEEEWDDVIDINLKGVFLCTKAVTRQMMKQRAGKIVNLASIVGVIGNPGQANYVAAK
                     AGVIGLTKTTARELATRNITVNAVAPGFITTDMTDALPEDVKEQMLGSIPLGKLGSAK
                     DVAGAVAFLLSDDANYITGQTIHVDGGMVM"
     gene            1938..2174
                     /locus_tag="VFX34_01465"
     CDS             1938..2174
                     /locus_tag="VFX34_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5563600.1"
                     /translation="MLSVLERVTKVVVDRLGVDESEVKPEASFKDDLGADSLDVVELV
                     MELEDEFDMEISDDDAEKIGTVGDAVKYIEEKVN"
     gene            2346..3113
                     /gene="rnc"
                     /locus_tag="VFX34_01470"
     CDS             2346..3113
                     /gene="rnc"
                     /locus_tag="VFX34_01470"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5563601.1"
                     /translation="MTNKRNVKSKYPIVPLPSEVRKKFEDLQKRLDIQFKDVSLLYNA
                     FTHSSYVNEHRRKNFTDNERLEFLGDAVLELGVSRYLYATEPNMSEGELTKLRAAIVC
                     EPSLVKFSNELHFGDFILLGKGEEQTGGRLRPALLADVFEAYIGALFLDQGMEPVTRF
                     LEKVVFPKIGDGAFSHVMDYKSRLQEIVQQTNSGSLNYEIVEEKGPAHAKKFVTVVRL
                     ENRELGTGVGRSKKEAEQEAARQAIQELKGNEAEGEN"
     gene            3116..>3642
                     /locus_tag="VFX34_01475"
     CDS             3116..>3642
                     /locus_tag="VFX34_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5563602.1"
                     /translation="MFLKRVEIIGFKSFAERIGIDFVPGVTAIVGPNGSGKSNITDAI
                     RWVLGEQSAKSLRGAKMEDVIFAGSDSRKPLNYAEVTLILDNTNGLFPLDYTEISVSR
                     RVFRSGESNYLLNGQQCRLKDINDVFMDSGLGKEAFSIISQGRVDEILNSRPEDRRSI
                     FDEAAGVLKYKTRKR"
BASE COUNT         1052 a          762 c          961 g          867 t
ORIGIN      
        1 cggaatgacg aaagtagcgt ttatattccc gggacaaggc tcgcaatctg ttggaatggg
       61 ttcggaacta ttcaatggtg gcgggaaata ccttgaacgg gctgatgaaa tattgcaatt
      121 cggcttaagc cgtttaatta cggaaggacc tcaagaggag cttacattga cgtataatgc
      181 gcagcctgcc ttgttgacag taggcgccat gatcgcttca aggctcatcg aggaagggat
      241 caaacccgat tatactgccg ggcatagcct tggcgagtat acggcgcttg tcgcttcagg
      301 agtcctttca ttcgaagatg gcgtgtcagt tgtccataag cgcggcctat tcatgaatga
      361 tgccgttcct gccggcgagg gagcgatggc tgccattcta gggctggatg gcgaaaagct
      421 acaagaagtg actgattcca ttacagcaga agggaatccg gtacaacctg ccaacttgaa
      481 ttgtcctgga caaattgtca tctcgggcac gaaagcgggc gttgaaaaag cttgtgtaga
      541 gcttaaggaa gcaggagcga aaagggcgat accccttgat gtgagcggac cattccactc
      601 ctcccttatg aagcccgcag cggaacgcct gaaaaatgca ttggatgaag tggaaatgaa
      661 agatgcctca attcctgtcg ttgcgaatgt gaatgcagaa acggtatccg acagtgagga
      721 gatcaagcaa ctacttgtgg aacaattata ttcccctgtc cgttgggaag actccgtacg
      781 cgaactgatc aaccttggag ttacgcattt cattgaatgt ggccctggaa aagtgctcag
      841 cggccttgta aagaaaatcg atagatctgt tacagtgctt cctgtctatg acgaagagac
      901 attgcaaaca gtcatcgatg cagcgaaagg gtgggaatga tgggaagatt tgaagggaaa
      961 acagcgattg tcacaggcgc ctctagagga atcggacgtg agattgcaat tttgctagga
     1021 aaagaaggtg cccgtgtagc tgtcaattac agtggcagca aagaaaaagc cgaagaagtc
     1081 gttgaattga tcaaacaatc cggcggagac gcttttgcca tccaagcgaa cgtgtcggat
     1141 gccgaacaag tgaaacagat gatcgatgag acattgaaaa cattcggctc aattgatatg
     1201 ctcgttaata atgcaggcat tacgcgtgat aacttgctaa tgcgcatgaa ggaagaagaa
     1261 tgggatgatg tcatcgacat caatttaaaa ggcgtcttcc tatgcacaaa agccgtaaca
     1321 cgccaaatga tgaagcaacg cgcagggaag atcgtcaacc tcgcatcgat tgtcggcgtt
     1381 atcgggaacc cgggccaggc caattatgtc gctgcaaaag ccggcgtcat cggtttgacg
     1441 aagacaactg caagagaact cgctacaaga aatatcaccg tgaatgcagt cgctcctggt
     1501 ttcatcacaa ccgacatgac cgacgccctt ccagaggacg taaaggagca aatgttaggt
     1561 tccattcctt taggtaaatt aggttcagct aaagacgtag cgggcgcagt agcgttccta
     1621 ctgtcagatg atgcaaatta tattacagga cagactattc acgttgatgg cggtatggta
     1681 atgtaagtac ctgtacaatt ttaatgtcca gctacagcgg ccaggtgctc gggtcataag
     1741 ccagcccagc tgtgcggcaa aaagcctgtc gcacctaagc aggccgcttg cgcttttcat
     1801 gtgtccagct tcgggcgcta gcccctcgag tcgcttcggt cttgaagcaa aaggcaaagt
     1861 gcgccttttg tccaagccct ccagcgcttg tcggggctga gcaagcgccc tcagcatttc
     1921 attgagagga ggtgactatg ttgtcagtac tagaacgtgt aacgaaagtt gttgtcgacc
     1981 gtcttggtgt cgatgaaagc gaagtgaaac ctgaagcttc tttcaaagac gatcttggcg
     2041 cagactcttt ggatgtcgtt gagctcgtaa tggaactaga agatgaattc gatatggaaa
     2101 tttccgatga cgatgccgaa aagatcggaa cagttggaga tgcagtgaag tatatcgaag
     2161 aaaaagtgaa ctaatttcag taagtatccg aaaaaaatgt gcttatttca catagcgcac
     2221 ttccatggag atgctttcac cctttcccac gaaaagaaag ggtgaaagta tctcttttcg
     2281 gttttgcatg tatggcttta acaatgtaaa attaaaagga ctacgaagtg aaaggcagaa
     2341 tcttgatgac taacaaacgg aatgtaaaaa gcaaatatcc tatagtcccg cttccaagcg
     2401 aggtgcgcaa aaaatttgaa gacctacaaa agcgtctcga catccaattt aaagatgtat
     2461 ctcttcttta caatgcattc actcattcat catatgtcaa tgagcaccgc cggaaaaact
     2521 tcacggataa cgaaaggctt gaattccttg gagatgccgt actggagctc ggcgtttcac
     2581 gctaccttta cgctaccgaa ccgaatatga gtgaaggcga gttgacaaaa ctgcgcgcag
     2641 ccatcgtttg tgagccatcg ctcgtcaagt tttcgaatga actgcatttc ggtgatttca
     2701 tcttacttgg caaaggcgag gaacagaccg gcggcagatt gcgcccggca ttgcttgcag
     2761 acgtatttga agcatatatc ggtgcattat ttttggatca aggcatggaa ccggtcaccc
     2821 gattccttga gaaggttgta ttcccgaaaa ttggggacgg tgctttttcg catgtgatgg
     2881 attataaaag ccgcttgcag gaaatcgttc aacagacgaa tagtggcagc ttgaattatg
     2941 aaattgttga agagaagggt cctgcgcatg cgaaaaagtt tgtcaccgtc gttaggttgg
     3001 aaaatcgtga acttggaacc ggagtcggta ggtcgaaaaa agaagccgaa caggaagcgg
     3061 cccgtcaagc cattcaagaa ttaaaaggaa atgaggccga aggggagaac tgatggtgtt
     3121 tctgaagaga gttgaaatta tagggttcaa atcatttgcc gaacggatcg gcattgattt
     3181 cgttcctggc gtgacggcca tcgtaggacc gaatggcagt ggaaaaagca atatcaccga
     3241 tgcaatccgt tgggtgctcg gtgaacaatc ggctaaatcc ctcaggggtg caaaaatgga
     3301 agatgtcatt tttgcaggga gtgattcccg caagccgctg aattacgctg aagtgacact
     3361 gatccttgat aacacgaacg ggcttttccc gctcgattac acggaaatca gcgtaagccg
     3421 ccgagtcttc cgttcggggg aaagcaatta tttattgaat ggacagcaat gcagattgaa
     3481 ggatatcaat gatgtcttca tggattcggg attagggaaa gaagcttttt caatcatctc
     3541 ccaaggccgt gtagatgaaa tccttaacag ccggccggaa gataggcgga gtatttttga
     3601 cgaggctgca ggtgtactga agtataaaac acgtaaaagg aa
//
LOCUS       DASULI010000066         8971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_180741, whole
            genome shotgun sequence.
ACCESSION   DASULI010000066 DASULI010000000
VERSION     DASULI010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8971
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180741"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFX34_01480"
     CDS             <1..191
                     /locus_tag="VFX34_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage VI sporulation protein F"
                     /protein_id="HEX5563603.1"
                     /translation="FALANAIQYADFSDERQVRKIVRKVGRLANKEVPPHMEDELVKS
                     IVNNGNSVNFNDIQRMMG"
     gene            525..830
                     /locus_tag="VFX34_01485"
     CDS             525..830
                     /locus_tag="VFX34_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563604.1"
                     /translation="MGYILPVQPTQGELYANRMNAVRNNFAYVDRVEKVKMDPDLMDK
                     FEDSLRSEQERMMEEEEPKIVQSRPPYLKGFIYPNPANLSPDIAHLVGKGLAVNEYV"
     gene            complement(921..1361)
                     /locus_tag="VFX34_01490"
     CDS             complement(921..1361)
                     /locus_tag="VFX34_01490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5563605.1"
                     /translation="MIEVKIARSDREREDAYDVRKKVFVEEQGVPISLEIDEFDNTAS
                     HFIVYDEKRPIGAGRIREIGPGIGKVERVCVLKELRGKHLGNLIMHELERHAKSAAMT
                     KIVLNAQSYAVPFYEKLGYTVTSPEFMDADIPHRAMEKEIAPLS"
     gene            complement(1365..1880)
                     /locus_tag="VFX34_01495"
     CDS             complement(1365..1880)
                     /locus_tag="VFX34_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HEX5563606.1"
                     /translation="MKYGVVAFPSKKLQDLANGYRKRYDPHYALITPHMTVKGVFEAD
                     DKEIEQVAEAIHEVVRNHKPFELKVTKVSTFAPITNTIFFKVTPNEEILSLHKDLNED
                     FFGDKPEYSFVPHITIAQKLNSGEHDDIIGQIKMFGVDHTEVIDRIHLLYQLEDGSWT
                     VYETFRLGEES"
     gene            complement(1964..2689)
                     /locus_tag="VFX34_01500"
     CDS             complement(1964..2689)
                     /locus_tag="VFX34_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEX5563607.1"
                     /translation="MEYGKIEEVFFYSKALDEEMQLLIHLPHQYSPLYKYSVLIASDG
                     KDYFQYGRIGRVVDELVDEGDISNMIVVGIPYKSVKERRRMYHPEGDRHEDYIRFLAH
                     ELVPYIDENYPTYQVGAGRGLIGDSLAATISLLTASKYPNCFGKVILHSPYVDDYVLE
                     KVEAVKDPSAFSIYHVIGEEETDVKTTQDGTQDFLTPNRELNKLIKRKGFSCFYEEFK
                     GNHTWKYWQQDVPRAIKYSFRDI"
     gene            2869..3414
                     /locus_tag="VFX34_01505"
     CDS             2869..3414
                     /locus_tag="VFX34_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX5563608.1"
                     /translation="MFTGKKIVHSDVVTKATREALIRRGVTMEDIAKIVYEMQVPYNE
                     GLDLAECVDSVEKVLRKRELQHAILVGIELDELAEQGKLSAPLQQIVESDEGLFGVDE
                     TIALGAVFTYGSIAVTTFGHLDKNKIGIINELDTKKGKGVHTFLDDLVASVASCAASR
                     LAHRKRDLDEAGITYLDADPN"
     gene            3586..4572
                     /locus_tag="VFX34_01510"
     CDS             3586..4572
                     /locus_tag="VFX34_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5563609.1"
                     /translation="MNKKRFRILAFISMIALLVLSACGADDSSKGSGSDSGKTKEDYD
                     FLSVLTGGTQGTYYALGGALADIITQETGVKTTAEVSQASAANMTALKDGGAEIAFVQ
                     TDIAYYASKGEMMFEGEVIDEVSAIGALYPETVQLVTLEKSGIKSFDDLKGKKVSVGA
                     PGSGTYANAEQLLEIHGLTMKDIQPQDLDFGESQESLQAGQIDAAFITAGTPTGAVEG
                     LNATAQVYIVPVSDEKADELISKYQYYAKEVIPAGTYGIKEDTPTVSVGAMLVIQNDV
                     PEDLAYEITKAIYDNASKLTHPKGALIKAETGLDGIGIPVHPGAQKYFDEKK"
     gene            4661..5182
                     /locus_tag="VFX34_01515"
     CDS             4661..5182
                     /locus_tag="VFX34_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HEX5563610.1"
                     /translation="MKRKNVILPILLSALFVALVSIFLPIHKVFTFTEYRTDHPKTYY
                     IRMEDEREFQIRYVHSIFLTDVVESYRATDQNTIQSLSMQYEDVGIGLPGYAEEGESL
                     SVRDGMYTLTHEHNILPSFVLFVGDVNADLAFRYKGQEVDLKNYLTRGKSYTFRVQRQ
                     SFYQLMKGVNMNG"
     gene            5175..7214
                     /locus_tag="VFX34_01520"
     CDS             5175..7214
                     /locus_tag="VFX34_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX5563611.1"
                     /translation="MAKNKNREEIQQESQIDNNEMLTEEQQLEILQKYDPESNTRNVK
                     GIFKVIVFAGLLAFSLFQLYTSIGTPFTAYIQRSIHLGFALALIFILFPAIKKPGVKR
                     DKVPFYDVILSLLAVAVGLYWPLFYGDLVFRVGRVSDIDLFIGVVAILLTLEAARRAV
                     GLPITIISTVFLIYAFFGQYFPGFLAHRGQDLKSIVQLMFFTTDGILGTPISVSATFI
                     FVFLLFGAFLVKTGVGNYFNDLAVVLAGRLTGGPAKVAIFSSALQGTISGSSVANVVG
                     SGSYTIPMMKKLGYRKEFAGGVEAAASTGGQIMPPIMGAAAFLMVEFIGGITYWEIAK
                     AAAIPALLYFTGIWIMTHFEAKRVGLEGMSPDQMPDRKATLKKIYLLLPILGIILFLL
                     LGIPTMKAALLGIVLTLVVSFFDKSTRLGPKDIIMALVDGARTALAVAAATACAGIIV
                     GVVVKTGLGLSLANGLVSAAGGNILLTLIFTMLAALVLGMGSPTTANYVITSTIAAPA
                     IILLLMGGEMGPGTAVPVVVAISAHLFVFYFGIIADITPPVALAAFAASGISGGDPIK
                     TGVVSAKLAIAAFIIPYMFVFNPAMLMIDSSFLEIIWVVFTAIVGMIAIGAGMIGYWY
                     RKLNWIERIIITITGLLLIYPETITDIIGLSLFVIQLAIQWKTKNNKPNNKEVIA"
     gene            7411..8307
                     /locus_tag="VFX34_01525"
     CDS             7411..8307
                     /locus_tag="VFX34_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563612.1"
                     /translation="MLKLSNVSITYNEKAVLDQLDLTANIGEIIGVAAPNGTGKSTMF
                     NVMANFVKPDSGHVTFDGKYAYRNEKEELLIHKMLCTFPEQKDLFEELSGVDHLKLYA
                     NMWNGSAKHVPEVIERLHMGNYVKRKARTYSLGMRQRLCFAMMMAADTPVMLMDEVMN
                     GLDVDNVALISECLMEMKKDKLIFVASHLLSNLDLYADRVLFLKDGKFVHEHRFTGEN
                     DVYLKIEITPLQYETLKEKVTLPKDYIFLANHLLCIPLKGMSASEQTKWIETMLDYNE
                     KELTVGPLGTVEYYDKFYSKNV"
     gene            8347..>8971
                     /locus_tag="VFX34_01530"
     CDS             8347..>8971
                     /locus_tag="VFX34_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563613.1"
                     /translation="MNRNYLKHEFLITLRSRRNIPFLLFIGVLLLSYCLILLPNEKTK
                     ESYEPDEVKTYLTDLEVEQQFREAKGNTGIILRTGMPVYAMNAHYYKLNHALLTAYED
                     KNYPRFLYLRNYTLLGNEYEYTSDEDLFALSPYPGKDRSHLYKQTMMRYEDYLTNDYP
                     ITYGLMYEKTGLQALQKFLQTNGILFILFCAIFFSSDMLSRDRKNRSV"
BASE COUNT         2540 a         1922 c         2098 g         2411 t
ORIGIN      
        1 tttttgcatt ggcgaacgca atccaatatg cagatttcag tgatgagcga caagtccgga
       61 aaatcgtacg caaagtcggc cggctcgcaa ataaagaagt cccgccccat atggaagatg
      121 aactcgtgaa atcaatcgtc aacaatggca attccgtaaa cttcaacgat attcaaagaa
      181 tgatggggta aacaaaagcg gaggacgcct gcctagatgc gacaggcaaa gccgtaacga
      241 agaacggctt ttgcgagtaa aagcgaagcg ttaggagcaa tattctttgc cacacagcag
      301 ctttgactta tgacccgagc agctggcgtc cgtagctggc tgaaatctta cccctatccc
      361 gcatgatcat cacttcccca atggacctag ctgaggaaat acgtttaacc tatgacgcaa
      421 cagaaaaact agtagaaacc aatcattttc cgccaactac gtttcataca ctcgtaaaaa
      481 ggttaaaggg taaagcgagg ataaatgtaa aggggtgtgg ggaaatgggc tatattttac
      541 cggtccagcc gacgcaaggc gaactatacg ctaatcggat gaatgcagtg cgcaacaatt
      601 ttgcgtatgt cgatcgggtg gagaaagtga aaatggatcc ggacctgatg gataaattcg
      661 aggattcact ccggagtgaa caggagcgga tgatggagga agaggaaccg aagatcgtcc
      721 aatcccgacc tccatacttg aaagggttca tctatccgaa tcctgccaac ctatcgcctg
      781 atattgcaca tctcgtagga aaagggctcg cagtgaatga gtatgtgtga tgggacgaag
      841 catttaactt gaaatccaaa aaagagcaga tccgcaaccg tactatccgg tatgcggatc
      901 tgctcttttc aattggggta ttacgataaa ggagcaatct ccttttccat tgcacggtgc
      961 ggaatatccg catccatgaa ctcgggcgac gtgaccgtgt acccgagttt ttcatagaat
     1021 ggcacagcat aagactgtgc attcaagacg attttcgtca tagcagcgga tttcgcatgt
     1081 ctttcaagtt catgcatgat cagattcccg agatgcttcc cacgcaattc cttcaataca
     1141 catacccgtt ccactttccc gatccctggg ccgatttccc ggattcgtcc tgctcctatc
     1201 ggccgctttt catcatagac gatgaaatgg gaagctgtat tatcgaactc atcaatttca
     1261 agactgatcg gtactccttg ttcttccaca aaaacttttt tccgcacgtc atacgcatct
     1321 tcccgttcac gatcggaacg ggcaatcttt acttcaatca atcttcagct ttcctcgccg
     1381 agacggaaag tttcataaac tgtccaagac ccgtcctcaa gttgatagag aagatggatg
     1441 cgatcgatta cttcagtatg atctacgccg aacatcttga tttgaccgat gatatcatca
     1501 tgttcaccgg agttcagctt ttgcgcgatt gtgatatgcg ggacgaagga atattcaggt
     1561 ttatcaccga aaaagtcttc attcaggtct ttatggagtg aaaggatttc ctcattaggt
     1621 gtcactttaa agaaaatcgt gttggtgatg ggagcgaatg tgctcacttt tgtcactttc
     1681 aattcaaacg gcttatgatt gcggacaact tcatggatgg cttccgcaac ttgttcgatc
     1741 tccttatcat ccgcttcgaa tacacctttg acggtcatgt gcggcgtgat aagcgcataa
     1801 tgtggatcat aacgtttcct gtacccgttt gccaaatctt ggagtttctt tgaagggaaa
     1861 gctacaacac cgtatttcat aataaaagac ctccttgtag aatgtttaca tacaacttat
     1921 aaacaattat agcagaatga actattcttt gtactgtttg gtatcaaatg tcgcgaaagg
     1981 aatactttat cgcccttggg acatcctgct gccaatattt ccatgtatgg ttccctttaa
     2041 attcttcata gaaacaggaa aacccttttc ttttgatcaa cttatttaat tccctgttcg
     2101 gtgtgaggaa atcctgggtg ccgtcctgag tcgttttgac atccgtttcc tcctcgccga
     2161 tgacgtggta aattgaaaat gcggaaggat ctttcacagc ctccactttt tccagcacat
     2221 agtcgtccac gtatggggaa tggagaatca ccttgccgaa gcagttcgga tatttcgatg
     2281 cggtcagcag tgaaatcgtc gctgccaggg aatcgccgat caaccccctg ccggcaccga
     2341 cttggtatgt aggataattc tcatctatat aagggacaag ctcatgcgct agaaatcgga
     2401 tgtaatcctc gtgccggtcg ccttccggat gatacatcct tctgcgttcc ttgacgctct
     2461 tataaggtat gccaacaaca atcatattgc ttatatcgcc ttcatcgaca agttcgtcga
     2521 cgacccgtcc aatccgtcca tattgaaaat aatccttgcc atcggaggcg atcagcactg
     2581 aatacttgta aagtggtgaa tactgatgag gcagatggat gagaagctgc atctcttcat
     2641 caagggcctt gctgtaaaaa aacacttctt caatttttcc atattccatc gggcaggtcc
     2701 tccctgttgt ttgatgtaag aagattgtaa cataagcgca agtacaggta taatgcttgg
     2761 atagcctgtt gcatgctaaa ggaaacaaac attgcttgaa taatcatcct gaaggctttt
     2821 gtcatataat acgtggcggg cgaagggtag aataaggagg tagtcattat gttcactgga
     2881 aagaaaattg tccattccga tgtcgtaaca aaagctacta gggaggcgct gatcagaaga
     2941 ggcgtcacaa tggaggacat cgcaaaaatc gtatacgaaa tgcaggtccc ttataatgaa
     3001 gggcttgacc ttgccgaatg tgtggattca gttgaaaaag tgttgcggaa gagggaactt
     3061 cagcatgcaa tccttgtcgg aatcgaattg gatgagcttg cggaacaagg gaagctctcg
     3121 gcgccgctcc aacagatcgt cgaatccgac gaaggtcttt ttggtgtgga tgaaacgatt
     3181 gcattggggg ccgtcttcac ttatgggtcc attgcagtga cgacattcgg ccatctcgat
     3241 aagaataaaa tcggcatcat caatgaattg gacacgaaaa aagggaaagg cgtccatact
     3301 ttcctcgatg atctcgtcgc aagcgtcgct tcatgtgcgg cttcacggct cgctcaccgg
     3361 aagagggact tggacgaagc gggtattacg tatttggatg cggatcccaa ctaaccaata
     3421 tgagtcattt agtggaattt gaaggaattc cacctccttt tttcctattg gaatcgcttg
     3481 caagttactg aaaattttta agaaggattg acaagcgata atccgttcgt ggtagaatgg
     3541 gtgttaagtt gcaaaactac cataatcaaa aggggagaat gaaatatgaa taaaaagaga
     3601 tttcgtattc ttgcgttcat ttcgatgatt gcgctgctgg tcctttcagc gtgtggcgcg
     3661 gatgattcaa gcaagggatc tggctctgac tctggcaaaa caaaagaaga ctatgatttc
     3721 ctaagtgttt tgacaggtgg tacgcaaggt acatattacg cacttggtgg agcacttgca
     3781 gatatcatca ctcaggaaac cggtgttaaa acaacagctg aagtttcaca agcatccgct
     3841 gcgaatatga ctgcattgaa agatggcggc gctgaaatcg ctttcgtaca gacggatatc
     3901 gcttactatg catccaaagg cgaaatgatg tttgaaggag aagtcatcga cgaagtttcg
     3961 gcaatcggtg cactttatcc tgaaacagtt caattggtca cactagagaa gtccggcatc
     4021 aaatcatttg atgatttgaa aggtaaaaaa gtatccgtag gagcgcctgg ttccggtacg
     4081 tacgcgaacg cagagcagct tcttgaaatc cacggattga caatgaaaga tatccagccg
     4141 caagacttgg atttcggtga atcccaagag agcttgcaag ctggacaaat cgatgctgca
     4201 ttcatcactg ccggtacgcc gactggtgcg gttgaaggtt tgaacgctac agcacaagtt
     4261 tacatcgtgc ctgtctccga tgaaaaagct gacgaattga tttcaaaata ccaatactat
     4321 gctaaagaag tcattcctgc aggaacttat ggcatcaaag aagatacgcc gactgtatct
     4381 gtaggagcga tgttggtcat ccaaaacgac gttcctgaag atcttgctta tgaaatcaca
     4441 aaagcgatct atgataatgc ttcaaaactt acgcatccaa aaggcgcttt gatcaaagcg
     4501 gaaacaggtc ttgatggtat cgggatccct gtacacccag gcgctcaaaa gtacttcgac
     4561 gaaaagaaat aattgaatac aagtcaatag ttgaacgggg cggtgaagct aaatgcttca
     4621 gccgtcccat tcttcattgc taccggaaag ggagaagaag atgaaaagga agaatgtgat
     4681 tctccccatt ctactctctg cactgttcgt tgccctcgtc tccattttcc tgccgataca
     4741 taaggtgttt acctttacgg aatatcgaac tgatcatcca aaaacctatt acatcaggat
     4801 ggaggatgag cgggaatttc aaatacggta tgtacactcc atttttctga ccgatgtcgt
     4861 cgaatcctac cgggccactg accaaaacac catccaatca ttgtccatgc aatatgaaga
     4921 tgttggaatc ggcttgcccg gctatgccga agaaggggag tcgctgtctg tcagagatgg
     4981 aatgtatacg cttactcatg agcacaacat cctcccttca tttgtcctct ttgtagggga
     5041 tgtcaatgca gaccttgctt tccgttataa agggcaagag gtggacttga aaaactattt
     5101 gactagagga aaatcgtaca catttcgtgt gcaacgccaa tcattctacc aattgatgaa
     5161 aggagtaaat atgaatggct aaaaataaga atcgggaaga aatccagcag gaatcccaaa
     5221 ttgacaacaa tgaaatgctg actgaagagc agcaactgga aattcttcaa aaatatgatc
     5281 ccgaatcgaa tacacggaat gtgaaaggga tatttaaggt tatcgtcttt gcaggattac
     5341 ttgcattttc cttattccaa ttgtacacat ccattggaac gcctttcacg gcatatattc
     5401 agcgttccat tcacttagga ttcgcgctcg cattgatttt catcttgttc ccggcaatca
     5461 aaaagccagg cgtcaagaga gataaagtac ctttctacga cgtcatcctc tcattgctag
     5521 ccgtggccgt gggattatac tggccgttat tctatggtga tctcgtcttc cgggtcggaa
     5581 gggtatccga tatcgatctg ttcattggag tcgtcgcgat tctattgaca ttggaggcgg
     5641 caaggcgtgc ggtcggtctg ccgattacaa tcatctcgac agtcttcttg atctatgcat
     5701 ttttcggcca gtatttcccg ggcttccttg ctcaccgcgg tcaagacttg aagagtatcg
     5761 tacagttaat gttcttcacg actgatggga ttttaggaac gccgatcagc gtttccgcga
     5821 cgttcatttt cgtcttcctg ttgttcgggg cattccttgt gaaaaccggc gtcgggaatt
     5881 acttcaatga tttggctgtc gtcttagctg gccgtttgac aggcggaccg gcgaaagtcg
     5941 cgatcttctc aagtgcattg caagggacga tttcaggaag ctccgttgca aacgttgttg
     6001 gctcaggttc gtatacgatc ccgatgatga aaaagttagg ttaccgtaag gaatttgctg
     6061 gcggtgttga agcagctgct tcgacgggcg ggcaaatcat gccaccaatc atgggggctg
     6121 cggctttcct gatggttgaa ttcatcggcg gcataacgta ttgggaaatt gctaaagcag
     6181 ctgcgattcc agcattgctt tacttcactg gaatttggat catgacgcat ttcgaggcga
     6241 aacgtgtagg tcttgagggc atgtcacctg accagatgcc ggatcgcaaa gcgacgttga
     6301 aaaagatcta cctgctcttg ccgattttag ggatcattct cttcctatta ttaggtattc
     6361 cgacaatgaa agctgcattg ttggggattg tcctaaccct agtcgtcagc ttctttgata
     6421 aatctactag acttggaccg aaagatatta tcatggcact cgtcgatgga gcgcgaacag
     6481 cattggcagt tgccgcggct accgcttgcg caggtatcat cgtcggcgtc gtcgtgaaaa
     6541 caggattggg gctcagcctt gcaaacggac ttgtttccgc agcgggtgga aatatcctct
     6601 tgacgctcat cttcacgatg ttggcagcgc ttgtcctagg gatgggttca ccgacaacgg
     6661 cgaactacgt cattacgtcc acaatcgccg cgccggccat catcctattg ctcatgggcg
     6721 gcgaaatggg gccagggacg gcggttccag tcgtagtcgc catctctgct cacttgttcg
     6781 tgttctactt cggtatcatc gcggatatta cgccgccagt tgccctagct gcattcgccg
     6841 catcaggcat ttccggaggg gatccgatca aaacgggagt cgtttccgcg aagcttgcca
     6901 ttgcggcatt catcattcca tatatgttcg tattcaaccc ggccatgcta atgatcgact
     6961 ctagtttcct agaaatcata tgggtcgtct tcaccgccat cgtgggcatg attgcgatcg
     7021 gagcaggaat gatcgggtat tggtaccgta agctcaactg gatcgagcgg atcatcatta
     7081 caattacagg gctgctgtta atctaccctg aaacgattac ggatatcatc ggattgtcac
     7141 tattcgttat ccaactagcc atccaatgga agacgaaaaa taacaaaccg aataataaag
     7201 aagttatcgc ataaagaaaa ggctgtccca attgtcacta gacaactggg tcagtctttt
     7261 ttatgccgat ttccgagtga aatggcaata tcagtcgatt gatattacag aaaccattga
     7321 aatgttacaa ttatatgtct atagtgaaat ataataaaca aatatatgga aaatggtttg
     7381 aacgaataag aggggtaggt ggaaagatag atgttgaaat tatcaaatgt ctcaattacg
     7441 tataatgaga aagcagtcct cgatcaattg gatttaaccg caaatatagg cgagattatt
     7501 ggcgtggcgg ctcccaacgg cacagggaaa tcaacgatgt tcaatgttat ggcgaacttt
     7561 gtaaaaccag attctggaca tgttacattc gatggcaaat atgcatatcg aaatgaaaaa
     7621 gaagagctgc tcatccataa gatgttatgc acatttcctg aacagaaaga ccttttcgag
     7681 gaactatccg gcgtggatca tttgaagtta tatgcgaata tgtggaatgg gtctgccaag
     7741 catgtacctg aagtaattga gcgacttcac atgggtaatt atgtaaaaag gaaggcgcgg
     7801 acctattcac ttggaatgcg gcagcgtcta tgctttgcga tgatgatggc tgcagatacg
     7861 cctgtcatgc taatggatga agtgatgaac ggccttgatg ttgacaatgt tgctttaatc
     7921 tccgaatgcc taatggaaat gaagaaggat aagctgattt ttgttgcctc tcacttgtta
     7981 tccaacttgg atttatatgc agatagggtc cttttcctga aagacggtaa atttgtacat
     8041 gaacatcgat tcactggaga aaacgatgtg tatcttaaaa tagaaataac gcctctacaa
     8101 tacgaaacat taaaagaaaa agtgacactt ccaaaagact acatattttt ggccaatcac
     8161 ttattatgca ttccgctaaa agggatgagt gcgtcagagc agacaaaatg gattgagacg
     8221 atgcttgact acaacgaaaa ggaactgaca gtcggaccgc tgggaacggt ggaatattac
     8281 gataagttct attcgaaaaa tgtttaagtt agctccaaat cattaagacc aaaaggggaa
     8341 gctggaatga atcgaaatta tttgaagcac gaatttctta ttacactaag aagcaggcgt
     8401 aatattcctt ttcttttatt catcggtgta ttgctgctaa gttattgtct cattctattg
     8461 ccgaacgaaa aaacgaaaga atcgtatgaa cctgacgaag tgaagactta tttaacagat
     8521 ttagaagtgg agcagcaatt tagggaggca aaggggaata cgggcattat tttgaggaca
     8581 ggcatgcccg tctatgccat gaacgcgcat tattacaaat tgaatcacgc tttgctaaca
     8641 gcatatgagg ataagaacta tcctcggttt ctttatttga ggaactatac tcttctgggg
     8701 aatgaatacg aatatacatc ggatgaagat ctattcgccc tgtccccata cccggggaag
     8761 gataggtccc atttatacaa gcaaacgatg atgcgatacg aggattattt aacgaatgac
     8821 tatccaatta catacgggtt gatgtatgaa aagacgggcc tgcaagcatt gcaaaaattc
     8881 ctgcagacga atgggattct gttcatctta ttttgcgcca tctttttcag cagcgatatg
     8941 ttatcaaggg accgcaaaaa ccggtcagtt t
//
LOCUS       DASULI010000067         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_691543, whole
            genome shotgun sequence.
ACCESSION   DASULI010000067 DASULI010000000
VERSION     DASULI010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691543"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFX34_01535"
     CDS             complement(<1..287)
                     /locus_tag="VFX34_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX5563614.1"
                     /translation="MSSVAFHTLGCKVNHYETEAIWQLFKDAGYERSDFEKNADVYVI
                     NTCTVTNTGDKKSRQVIRRAIRRNPDAVICVTGCYAQTSPAEIMAIPGVDIV"
     gene            complement(304..1044)
                     /locus_tag="VFX34_01540"
     CDS             complement(304..1044)
                     /locus_tag="VFX34_01540"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX5563615.1"
                     /translation="MQRYFLETPFDEGGDAFISGEDGKHIAKVMRMEAGDRIIAVDDG
                     EAFISEITDILTEGVTITKVDGPLPSNEMPVKMTIVCGLPKGDKLDLIVQKGTELGMS
                     KVIPFEAERSIVKWDAKKGDKKVERLKKIAKEAAEQCHRTMIPDVLAPMTFKQILQES
                     QNYDVRFFADEEDAKGNDPNRIADRLKNVYHKQSVLVVFGPEGGLSRKEADEMRAAGF
                     LSVSLGPRILRTETAPLYFLSAMSYEFE"
     gene            complement(1048..1992)
                     /gene="prmA"
                     /locus_tag="VFX34_01545"
     CDS             complement(1048..1992)
                     /gene="prmA"
                     /locus_tag="VFX34_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5563616.1"
                     /translation="MKWSEISIHTSHEATEAVANILHEAGASGVIIEDSAEPDRIHED
                     RFGEIYELKKEDFPTEGVIVKAYLPVNSFLIETVKEISESVNELAEFGLDVGLNQVQT
                     NEVDEEDWATAWKKYYHPVKISGRFTIVPTWEIYEPVDSDELIIELDPGMAFGTGTHP
                     TTVMCLQALEKYVKKGDSVIDVGTGSGVLSIGAALLGASRVHALDLDEVAVTAAKENI
                     SLNNVDDVVEVTHGNLLDSVKEPASIIVANILAEVIMTFSEDAYSILPEDGLFIVSGI
                     IAQKRDLVKDDLQSKGFEIIESVLMEEWVALIASKRSV"
     gene            complement(2008..>2412)
                     /locus_tag="VFX34_01550"
     CDS             complement(2008..>2412)
                     /locus_tag="VFX34_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017552610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX5563617.1"
                     /translation="NGGPAGDLYIVFRVKDHDRFVREGDDIYLEVPITFPQLALGDEI
                     EVPTVTGNVKLKIPAGTQTGTNFRLRGKGVKNVHGHGTGDQHVIIKAITPKKMTEKQK
                     ELLREFASIEGNSPDEYSSSLFDKIKRTIKGD"
BASE COUNT          572 a          635 c          506 g          699 t
ORIGIN      
        1 acaatgtcga ctccaggaat cgccatgatt tccgctgggg aagtctgtgc ataacaaccc
       61 gtgacacaaa ttaccgcatc cggattccgc cggatggctc tccggatgac ttggcggctt
      121 ttcttatcgc cggtattcgt gaccgtacat gtattgataa cgtaaacgtc tgcatttttt
      181 tcgaaatcgg agcgttcata gccggcatcc ttgaaaagct gccagatcgc ttccgtttca
      241 taatgattca ctttgcagcc taatgtatga aaagcaactg atgacactag ctgctcacct
      301 ctttcattca aattcgtatg acatagcgga caaaaaatag agcggggcag tttccgtcct
      361 taagatgcgc ggacctaatg aaaccgacag aaaacccgcg gcgcgcattt catcggcttc
      421 ctttcttgac aatccgcctt caggaccgaa aacgaccagt accgattgtt tatgatacac
      481 gtttttcaac cggtctgcaa tcctgttggg atcgttgccc ttcgcatctt cttcatcggc
      541 aaaaaagcgc acatcatagt tttgtgactc ttgcagaatt tgcttgaagg tcatcggagc
      601 taatacatct gggatcatcg tccgatggca ttgttccgcg gcctccttcg caatcttttt
      661 aagtctttcc acttttttat cgcctttttt cgcatcccat ttgacgatgg accgttccgc
      721 ttcgaaagga ataactttgg acatgccaag ctcggtccct ttttgaacga taaggtcgag
      781 cttatcgcct ttaggcaagc cgcatacaat tgtcattttg acgggcattt cattcgaagg
      841 aagaggacca tccacctttg ttatggttac tccttcagtc aggatgtccg taatttcaga
      901 aatgaatgct tcgccatcat ctacggcgat gatgcgatca ccggcctcca tcctcatcac
      961 tttggcgatg tgcttgccat cctcacctga aatgaaggca tctcctccct catcgaatgg
     1021 tgtttcaagg aaataacgtt gcaaaattca cacgctcctt ttggaagcga taagtgcaac
     1081 ccactcttcc attaagacag attcgataat ttcaaagcct ttgctttgga gatcgtcctt
     1141 cacaaggtcg cgtttttggg caatgattcc cgatacgatg aatagcccat cttcaggcag
     1201 gatggaatac gcatcttcgg aaaacgtcat gatcacttcg gcgaggatat ttgcaacgat
     1261 aatggatgca ggctctttta ccgaatcaag caagttgcca tgcgtcactt caacaacatc
     1321 atccacgttg ttcaagctaa tattctcttt tgcagccgtc acagccacct catccagatc
     1381 aagtgcatgg acccgggatg cccctagaag cgcggctccg atcgataaca cacctgatcc
     1441 agttcctacg tcaatgaccg agtcgccttt cttcacgtac ttttccaaag cttgcagaca
     1501 catgactgtc gtcgggtggg tccccgtgcc gaaagccatg ccagggtcaa gttcgatgat
     1561 cagttcgtca gagtccaccg gctcgtaaat ttcccacgta ggaacaatcg tgaatctgcc
     1621 cgagattttg acgggatggt aatatttctt ccaagctgtc gcccaatcct cttcatccac
     1681 ttcgttcgtc tgaacttggt tgagccccac gtccagaccg aactcagcca attcattgac
     1741 agattcacta atttccttaa ccgtttcaat caaaaagcta ttgactggca agtaggcttt
     1801 tacaataact ccctctgtag ggaaatcctc ttttttcagt tcatagattt cgccaaaacg
     1861 atcttcatga atccggtcgg gctctgcgga atcttcaata atgaccccgc tcgcccctgc
     1921 ttcatgcaaa atattggcaa cggcttcagt tgcctcatgt gacgtatgga tggagatttc
     1981 agaccatttc aactacgttc atccctttca gtcgcctttt attgttcttt tgattttatc
     2041 aaaaagtgag ctcgaatact catccggcga gttgccttca attgaagcga attcacggag
     2101 aagttctttc tgcttttccg tcattttttt aggtgtgatc gctttgatga tgacgtgttg
     2161 atcacccgtt ccatgtccat ggacattttt gacgcctttg ccgcgtagtc ggaaatttgt
     2221 ccccgtctgt gtacccgcag ggattttcaa tttgacgttg cctgtcacag taggcacttc
     2281 aatttcgtct cccagcgcca attgcgggaa tgtaatcggc acttccaaat agatgtcgtc
     2341 tccttcacgg acgaagcggt catggtcctt cactctgaag acgatgtata agtctcctgc
     2401 cgggccgcca tt
//
LOCUS       DASULI010000068         3401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_336533, whole
            genome shotgun sequence.
ACCESSION   DASULI010000068 DASULI010000000
VERSION     DASULI010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336533"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VFX34_01555"
     CDS             <1..1425
                     /locus_tag="VFX34_01555"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX5563618.1"
                     /translation="GFPASSPGDFEAVQRIANTVKNSTVTGLARAMKSDIDTSWEALK
                     GGQQPHLHVFLATSPIHMEHKLMKTPDQVVDIAVEAVKYAKKFFPLVQWSAEDAFRSD
                     PAFLVRIINQVIKAGATTINIPDTVGYATPQEYGALFKFLKENVTGIERVKLSAHCHN
                     DLGMAVANSIAAIENGADQVEGTINGIGERAGNAALEEIAVALHIRKDVYGFETGINL
                     QEIKRTSQLVSQLTGVVIQPNKAVVGKNAFAHESGIHQDGMLKNRETYEIITPELIGD
                     KTAPLALGKHSGRHAFKDRAVTMGFELSDAQLNEAFVEFKKLADRKKEITEDDLFVLF
                     TGKQIEHADADIYELTNVQVQYGTSNVPTATIAANVPTGENVVVATTGAGSVEAIFNA
                     LELLVKGQVNILDYRVTSIGKGRDALGEAVVNLRFNGETFTGRDISQDVLEASAKAYL
                     NAINRKLVKDQVKVQVKVMETVSQ"
     gene            1878..2978
                     /gene="leuB"
                     /locus_tag="VFX34_01560"
     CDS             1878..2978
                     /gene="leuB"
                     /locus_tag="VFX34_01560"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5563619.1"
                     /translation="MEKRVAVLQGDGIGPEVTSAAVQILEVVARRFNHTFHFNYGSIG
                     GAAIDSFNDPLPQETIELCESSDAVLLGAVGGPKWDRNPSELRPEKGLLAIRKHFDLF
                     ANLRPVKAVPSLLQASPLKEEIVREVDMMIVRELTGGIYFGQPSRRTGTSAVDTLVYT
                     REEIERIVEKAFELARMRRGKVTSVDKANVLESSKLWREVVEEKRERYPDVEVEHQLV
                     DSAAMKLITNPKAFDVVVTENMFGDILSDEASVITGSLGLMPSASVRSDGFGLYEPVH
                     GSAPDVAGMGIANPAAAILSAAMMLRYSFGMDTEAAAIEKAVETVFEDGHFTADLASD
                     ADRALSTTEWTDKVIGELDLQFVSNSIMFSYV"
     gene            2998..>3401
                     /locus_tag="VFX34_01565"
     CDS             2998..>3401
                     /locus_tag="VFX34_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5563620.1"
                     /translation="MPKTIIEKIWEQHVVYEEPGKPDLLYIDLHLLHEVTSPQAFEGL
                     RLAGRKVRRPDLCFATMDHNVPTRNLPTINDPIAKKQITTLEKNCEEFGIELAHMEHP
                     DQGIVHIIGPELGLTQPGKTIVCGDSHTSTHGA"
BASE COUNT          923 a          786 c          915 g          777 t
ORIGIN      
        1 gggttccctg cttcctcacc tggtgatttc gaagcagtgc agcgcattgc gaatacagtg
       61 aagaactcga cggtgaccgg gcttgcccgt gccatgaaaa gcgatatcga tacatcatgg
      121 gaagcgctga aaggcggaca acaaccccat ctgcatgtat ttctggcaac ttccccaatt
      181 catatggaac ataaattgat gaagacgcct gaccaagtcg tggacattgc agtggaagcc
      241 gtaaaatacg caaaaaaatt ctttcctctc gttcaatggt cagcagaaga cgcattccgt
      301 tccgaccctg cattcctcgt ccggatcatc aatcaggtga tcaaagccgg tgcgacgaca
      361 atcaatatcc ctgacacggt cggttatgca acgccgcaag agtacggtgc tttattcaaa
      421 ttcttgaagg agaatgtgac aggcatcgag cgtgtgaaac tatccgcaca ttgccataac
      481 gatttaggga tggctgtcgc caactccatc gcagcgattg aaaatggtgc cgatcaagtc
      541 gaaggcacga taaacgggat cggtgaacgg gctggaaatg cagcgctcga agaaatcgcg
      601 gttgctctcc atatccgcaa ggacgtctac ggttttgaaa cgggcatcaa tctacaggaa
      661 atcaagcgca caagccaatt ggtcagccag ttgacaggcg tcgtcattca gccgaacaag
      721 gcagtcgtcg ggaagaacgc attcgcgcat gaatcgggca tccaccagga cggcatgcta
      781 aaaaaccggg agacatatga aatcatcacg ccggaactga tcggtgataa gacagctccg
      841 ttagcgctcg gcaagcattc cggccgccat gcattcaagg accgtgccgt gacgatgggc
      901 tttgagctta gcgatgcaca gttgaatgaa gcgtttgtcg agttcaagaa gctcgcagac
      961 cgcaagaagg aaattacgga agacgatcta ttcgtcctct ttacaggcaa gcagatcgag
     1021 cacgcagatg cagacattta tgaattgacg aatgtgcaag tgcaatacgg cacgtccaat
     1081 gttccgacgg cgacgatcgc agcgaatgtc ccaaccggag aaaatgtggt tgttgcaact
     1141 acaggagcgg gttcagtcga agcaatcttc aacgcactcg agctcctcgt caaagggcaa
     1201 gtcaacatac tggattaccg tgtcacatcc attggcaaag gccgcgatgc attaggggaa
     1261 gccgtcgtca acttgcgatt caatggcgaa accttcacag gccgcgacat ctctcaagac
     1321 gtcctggaag cttccgccaa agcatacttg aatgccatta accggaagtt agtgaaggat
     1381 caagtgaaag ttcaagtgaa agtgatggaa acggtcagtc aataacgggg ttgtgaactt
     1441 gcgttatgcg tgaataaacg aatttatgcg ctttttacag catttatgcg tgaatgtagc
     1501 gatttatgcg aggatttact gatttatgcg caattcctgt gaattatgcg tgaataaatg
     1561 aaattatgcg cttctcacag tatttatgcg taattccagc atttaatgcg tttcttttat
     1621 tagtaattat atagctgatg taatttataa ggattggtgc gaaagccgtt acgaagaacg
     1681 gcttttgcga gcaaaagcgc agcgttgcga gcaggatgct gccttaattt ctgcaaaaac
     1741 cgcagaaata cggcaaatcg aactcttcgt tgttcgattg gaaagcgtcc gcctggagtg
     1801 gaaatccgga ttacttatac atcaatcagt ctgaagattt ggtgaatggg aagacgacaa
     1861 caaggaggag aagttcaatg gaaaaaagag tggctgtatt acagggcgat ggaatcggac
     1921 cggaagtgac gtcggcggct gtgcaaattc ttgaagtggt tgcaagacgg ttcaaccaca
     1981 cattccattt caattacggt tcaatcggag gggcggcgat cgattcattc aatgatccgc
     2041 ttccgcagga gacaatagaa ctctgtgaat caagtgatgc agtactctta ggggcggtcg
     2101 gcggtccgaa atgggatcgg aatccttcag agctgcgacc tgaaaaaggg ttgcttgcca
     2161 ttcggaagca ctttgattta ttcgctaacc tgagacctgt caaggcggtc ccgtccttgc
     2221 tccaagcttc tcctctaaaa gaggaaattg tccgggaagt cgatatgatg atcgtccgcg
     2281 aattgacggg cggcatctac ttcggccaac caagccgcag gaccggaaca tccgcggtcg
     2341 acacactcgt ctatacgcgc gaagaaatcg aacgcatcgt tgaaaaagca ttcgaactcg
     2401 cgaggatgag acgggggaaa gtgacatccg tcgataaggc gaatgtgctt gaatcgagca
     2461 aactttggcg tgaagtcgtc gaagagaagc gggaaaggta tccagatgta gaagtcgagc
     2521 atcagctcgt cgactcggcg gcaatgaaat tgattacgaa tccgaaagca ttcgatgttg
     2581 tcgttactga aaacatgttc ggcgacattt taagcgacga agcgtccgtc atcaccggtt
     2641 cgctcggcct catgccgtcc gccagcgtgc ggtcggatgg attcggccta tacgagcctg
     2701 tgcatggctc cgctccggat gttgcgggaa tgggcattgc gaatccggca gcagccattt
     2761 tgtcagctgc aatgatgctc cgctattcat tcggaatgga tacggaagcg gcggcaattg
     2821 aaaaagcggt tgaaaccgtg tttgaagatg ggcatttcac agcagacctt gcttctgacg
     2881 ctgatcgcgc cctttcgaca acggaatgga cggataaagt gatcggcgag ctggatctgc
     2941 agttcgtctc gaatagcatc atgttttctt atgtctgaaa tggaaggaga attttatatg
     3001 cctaagacga tcatcgaaaa gatttgggaa cagcatgttg tgtatgaaga accgggtaaa
     3061 ccggatctgt tatatatcga ccttcattta ttgcacgaag tgacttctcc tcaggcgttt
     3121 gaaggcttgc ggctggcggg gcggaaagtg cgccgtcccg acctctgctt cgcaacgatg
     3181 gaccataatg tcccgacccg gaatttgccg accatcaacg atccgattgc caaaaagcag
     3241 attacgacgt tggagaaaaa ctgcgaggaa ttcggcatcg agcttgcgca tatggagcac
     3301 cctgaccagg ggatcgtcca tattatcggc cctgaactcg ggttgacgca gccgggcaag
     3361 acgattgttt gcggcgatag ccatacgtcg acgcacggcg c
//
LOCUS       DASULI010000069         9501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1173337,
            whole genome shotgun sequence.
ACCESSION   DASULI010000069 DASULI010000000
VERSION     DASULI010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173337"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VFX34_01570"
     CDS             <1..721
                     /locus_tag="VFX34_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015008767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5563621.1"
                     /translation="VRQAMWYAMDNETIGKELYHGLRFPATTLIIPVFASFHDPELET
                     PSYDPEKAKALLDEAGYKDVDGDGFRENDKGEEFVLNFASMSGGETAEPIAQFYMQNW
                     ADVGIKVQLLDGRLHEFNSFYDMVEADDPKIDIYQGAWGTGSDPDPSGLYGRDAAFNY
                     TRYTSEKNDELLAAGTSEKAFDSDYRKDIYKQWQELMVDEVPVAPTVYRYYLHGVNKR
                     VVNYSIDPGSDLELYQMGVSE"
     gene            927..1751
                     /locus_tag="VFX34_01575"
     CDS             927..1751
                     /locus_tag="VFX34_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX5563622.1"
                     /translation="MKIYVSVDMEGITGLPDYTFVDSARHNYERARRIMTEEANAIIR
                     AAIGQGAEQVLVNDSHSKMNNLLVEELHPDADLITGDLKAFSMVQAIDESFDGAIFAG
                     YHARAGQPGVMSHSMTFGVRNMHIDDVAIGELGLNAYVAGYYGVPVIMVAGDDRACKE
                     AEELIPGIVTAAVKQSISRSAVKTLHPTKAHALIEEKTAEAIAKRSTIKPLVPPTNPV
                     LRIEFTNYGQAEWAALMPGCEIEPGTTIVKFEAKDILEAYRAMLVMVELAMQTKFC"
     gene            1765..2700
                     /locus_tag="VFX34_01580"
     CDS             1765..2700
                     /locus_tag="VFX34_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563623.1"
                     /translation="MAIYIVKRFFMMIATILIIATLTFFLMHSIPGSPFDEERTSNPT
                     IQANLEKFYKLDQPTHVQYVHYLKSIATFDFGPSIKKPNETVNKLLARGFPISFELGM
                     VTILVAVISGITLGTFAALRHNGIIDYAAMAFAVIGISVPNFVLATVLIQQVAVNWNL
                     LPPATWSSPLHMILPTLALATGPTAIIARLTRSSMLEVLTQDYMKMARAKGLSPMRIV
                     LRHALRNALMPIVTIMGTMLAGILTGTFVIEKIFAIPGMGKYFVESINNRDYPVIMGS
                     TVFYSAFLVFMLFLVDIVYGFLDPRIKLHRKEGDA"
     gene            2700..3638
                     /locus_tag="VFX34_01585"
     CDS             2700..3638
                     /locus_tag="VFX34_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563624.1"
                     /translation="MVSHQQNVQVPDEWFKPKGTDAEKAESVVRPSLSYWKDAWRRLR
                     KNKLAMGGLVFLIILTLFAIFAPILSPHNIETTQYAKQNLPPSSEHWFGTDDKGWDVF
                     TRTWHGARVSLFVGVAAALIDFFIGIIYGGISGYKGGRTDTVMMRIIEILYGLPHLLV
                     VILLMVIMGPSLTTIIVALTITGWVGMARIVRGQVLQIKNYEFVTASKSFGAKTPRII
                     RKNLLPNTMGPIIVQMTLTVPSAIFAEAFLSFIGLGIQAPVASWGVMANDALPVILSG
                     HWWRLFFPAFFISMTMFAFNVLGDGMQDALDPKLRG"
     gene            3748..4734
                     /locus_tag="VFX34_01590"
     CDS             3748..4734
                     /locus_tag="VFX34_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010289965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563625.1"
                     /translation="MLSIRDLHVSFKTFGGEVQALRGVSFDLYKGETLAIVGESGCGK
                     SVTANTIMGLIPQPAGRIKNGSVLFKGKDLTKLDKSEMRKVQGVDISMIFQDPMTALN
                     PTLKIGEQLTEGLRTHQKVSKAEAREKAIRLLEVVGIPNPEERMKQYPHQFSGGMRQR
                     IVIAIALICEPELLIADEPTTALDVTIQAQILELFDEIQERTGVSIILITHDLGVVAK
                     IADRIAVMYAGKVIEIGDKREVFYSPQHPYTKGLLASVPRLDLKEEELQPIEGTPPDL
                     FSPPVGCPFTARCPFAMEVCERVYPATTGISDTHAVDCWLQDERAKKVFGHN"
     gene            4790..6457
                     /locus_tag="VFX34_01595"
     CDS             4790..6457
                     /locus_tag="VFX34_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010289964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5563626.1"
                     /translation="MKKWLTFLMVAMLALVLAACTANKDAGKEPEKPADTTPTDEGKD
                     EGKTDETASGEKILLMNNGEEPTSFDPSIGFNAVSWSALNNIMEGLTRLDKDHKAVEA
                     TAEKIDISDDGLTYTFTIRDGAKWSNGDPVVAGDFVFAWKHMLNPETASPAAFLAYFI
                     EGAEAYNNGEGSADDIAIEAKDEKTFVVKLTSPNEAFLNIITNPSFFPINEKVASADP
                     TWHTEAATFVGNGPFKLSAWSHDVSFEFEKNEHYWDADAVKLDKVHWAMVNDSTTAYQ
                     MYQANELDVTGVPSEIADQLKDSPELRIDDQAGLYFYRFNTSMEPFTNKKIRQAFAFA
                     VDQEEIVNFVTKNGEKPAHGFVTYGFIGPDGKEFRDSVGDLVSFDPEKAKQLLAEGMK
                     EEGYSELPKVTITYSTSESHQNIAIALQSKFKEVLGVDAELQNVESGVFLSEQKEFKY
                     QMSRSSFLHDYADPVNALESFITGSSMNRTTWSNAEYDKLIADIKNETDENKRWELLK
                     EADKLLMDEMPVFPLYFYNSTSLEKEGVSGILRHPVGYVDLKYADKN"
     gene            6686..7609
                     /locus_tag="VFX34_01600"
     CDS             6686..7609
                     /locus_tag="VFX34_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX5563627.1"
                     /translation="MRIRPKRLQPGDTIGIVAPSSPPNQESLERSLAFLEQLGLKWKF
                     GQYVKNVNGYLAGTDDERLQDLEDMFTDPCISGIICAGGGYGSARYTDRLDLQLIREN
                     PKVFWGFSDITFLHTAIGLYSDLVTFHGPMLASCVGKDTFHELSAKMFQQLFEPKELH
                     YTEEISPLRAITGGSAHGELVGGNLSLLADTVGTKFEIDTKGKLLFIEDVGEEPYRVD
                     GMLNQLRMAGKLADAAGIVIGDFSNVEPKKQQRTLSLDEVFRHYLGGLEKPVVEGFKI
                     GHCEPHFAIPLGVDAKLDADQKTLTILPGVE"
     gene            7610..8716
                     /locus_tag="VFX34_01605"
     CDS             7610..8716
                     /locus_tag="VFX34_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX5563628.1"
                     /translation="MRIRSIETIPVAVPLTKPFKTALRTVTTAYAIYVKITSEDGRTG
                     WGEAPPTHVITGDSLGSITYAIEEVIAPQLIGKDLRNREEVFRLLKRSIVRNTSAKAA
                     IDMAIHDLLGQLAGLPLCQLLGGFREKIETDFTVSVNDAAEMADDAERYIADGFNVLK
                     VKVGIGDISEDIERIKAIRGRVGKGPKIRLDANQGWKPKEAVYAIGKMEDAGLAIELI
                     EQPVSADDIDGLQYVTNQTLTPIMADESVFSPKDALRVLERRAADLINIKLMKSGGIH
                     EALKINALAETYGVECMVGSMIETKLGITAAAHFAASQPNITRFDFDAPLMLAEDIVA
                     GGITYEGRVIRLPEGPGLGLDREGMGLIGKEAGK"
     gene            8713..>9501
                     /locus_tag="VFX34_01610"
     CDS             8713..>9501
                     /locus_tag="VFX34_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5563629.1"
                     /translation="MTELTVKMYTCAVPVATVWTSPASVREMDTDGVSNPIHLNKWLE
                     KLAYEERLDLCNGNRVQTQLLYGEPVIVDEIEGDWAKVIAVWQPSKKDERGYPGWVPV
                     RQLKKASPLKEAGGFVRVAVAKSQLWKPDGTPLMIVPFNTILPLTDESDNHFHVETPD
                     GEALLLKTDIVRAPSVHQFKKRKADAAVEEGLAYLDLPYFWGGMSSYGFDCSGFTYNM
                     LKACGHFIPRDAGDQAKSGEEVPMGKPERWEKGDLLFFANDEGKG"
BASE COUNT         2558 a         2128 c         2602 g         2213 t
ORIGIN      
        1 tgttcgtcaa gctatgtggt acgcaatgga caacgaaacg atcggtaaag aactttacca
       61 cggccttcgt ttcccagcta ctactttgat catccctgta tttgctagct tccatgatcc
      121 agaactagaa acaccatctt atgatcctga aaaagcgaaa gctcttcttg acgaagctgg
      181 atataaagac gttgatggcg atggattccg tgaaaacgac aaaggtgaag agttcgttct
      241 taacttcgca tctatgtccg gtggagaaac tgctgagcca attgcacagt tctacatgca
      301 aaactgggct gacgtaggta ttaaagtaca acttcttgat ggacgtctac atgagttcaa
      361 ctcattctac gatatggtag aagctgatga tccgaagatc gacatctacc aaggtgcatg
      421 gggaactggt tctgacccag atccatccgg tctatacgga agagacgctg cgttcaacta
      481 cactcgttac acaagcgaga aaaacgatga gcttcttgca gctggaacat ctgaaaaagc
      541 atttgattcc gactaccgta aagatatcta caaacaatgg caagagttga tggttgatga
      601 agttccggtt gcaccaactg tataccgtta ctacctacat ggtgttaaca aacgtgttgt
      661 taactactct atcgacccag gctctgacct agagttgtac caaatgggtg taagtgaata
      721 atacttgatt attgagcaac atcagtaatc tgcaagccgc cgtccgggaa accggatggc
      781 ggttttttat tttatttcaa gtgaatctag ttctttaatt ttagaaatcg tattgtaata
      841 ttcttaaaaa tttgatagga ttgaacatac caattactag actagactat caattcagag
      901 agctgaaggg gacgggggaa ctagaattga agatttacgt atccgttgat atggaaggga
      961 ttacggggct gccggattat acgtttgtcg attcagccag acataattat gaacgcgcgc
     1021 ggcgtattat gaccgaggaa gcaaatgcaa ttatccgtgc ggcaattgga caaggggctg
     1081 aacaagtcct tgtgaacgat agccattcca aaatgaataa tcttcttgtg gaagagcttc
     1141 atcccgatgc ggatttgata acaggagact tgaaagcgtt ttcgatggtg caggcgatcg
     1201 atgaatcgtt tgacggggcc attttcgccg gataccacgc tagggccggt cagccgggtg
     1261 tcatgagtca ttcgatgaca tttggcgtca ggaatatgca catcgatgac gtggcgattg
     1321 gcgagctcgg attgaacgcc tatgtggcag gctattacgg agtaccggtc atcatggtcg
     1381 ctggggatga ccgtgcatgc aaggaagcgg aagaattgat tccagggatc gtgacagccg
     1441 ccgtcaaaca gtccatctcc cgttcagccg tgaaaacgtt gcatccaacg aaagcgcacg
     1501 ccttgattga agagaagact gcggaggcga ttgccaagcg ctcaacaatc aagccgcttg
     1561 tgccgccgac aaacccggtt ttgcgcatcg agttcacgaa ctatggccag gcagaatggg
     1621 ctgcgcttat gccagggtgt gaaatcgagc cggggacgac aatcgttaaa ttcgaggcta
     1681 aagatattct cgaagcgtac cgggcgatgc tcgttatggt cgagttggca atgcaaacaa
     1741 agttctgcta agggggaatg gcgcatggca atttacatag taaagcgatt cttcatgatg
     1801 attgcgacaa tcctcatcat cgcgacattg acatttttct tgatgcactc aataccaggc
     1861 tcgccgttcg atgaagagcg gacatcgaat ccgacgatcc aggcgaatct ggagaaattc
     1921 tataagctcg atcaaccgac acatgtgcaa tacgtacatt atttgaagtc gattgcgacg
     1981 ttcgacttcg gaccttccat taaaaagccg aatgaaacgg taaataaatt acttgccaga
     2041 gggttcccga tttcattcga gctaggaatg gtgaccatcc tcgtcgccgt catatcaggg
     2101 attacattag ggacatttgc cgccttgcgg cataacggca ttatcgacta tgcggcgatg
     2161 gccttcgcgg tcatcgggat atccgtcccg aacttcgtat tggcgaccgt tttgatccag
     2221 caggtggcgg tcaattggaa tctcctcccg cccgctacat ggagcagtcc gcttcatatg
     2281 atcttgccga cattggcatt ggcgacgggt ccgacagcca tcatcgcgcg tctgacacgg
     2341 tcgagcatgt tggaggtgct gacgcaggat tatatgaaaa tggcgagggc gaaagggtta
     2401 tcgccgatgc gcatcgtact gcgacacgcg ctccgcaatg cactcatgcc gattgtaacg
     2461 atcatgggga cgatgctcgc ggggattttg acggggacat tcgttattga aaagattttt
     2521 gcgataccgg gcatggggaa atatttcgtt gaaagcatca ataaccggga ttatccggtc
     2581 atcatgggct cgaccgtatt ctatagcgct ttcctcgttt tcatgttgtt tctagtcgat
     2641 atcgtttatg gattccttga tccacgcatt aaacttcacc gaaaggaagg tgatgcgtga
     2701 tggtaagtca tcagcaaaat gtgcaagtcc cggatgaatg gttcaaaccg aaagggacag
     2761 acgcggaaaa agcggaatcg gttgtgcgcc cgtcgctctc ctattggaaa gatgcatggc
     2821 gcagattgcg gaagaacaaa ttggcgatgg gcggattagt gttcttaatc atcctgacgc
     2881 tattcgcaat cttcgctccg attctatcac cgcataatat agaaacgacg caatatgcaa
     2941 aacagaattt gccgccgtca tcggaacatt ggtttggaac ggacgacaaa ggatgggacg
     3001 tcttcacaag aacttggcac ggtgcccgcg tttcactatt cgtcggtgtt gccgccgcat
     3061 tgatcgactt cttcatcggc atcatctacg gggggatcag cggttataaa ggcggaagga
     3121 cggacaccgt catgatgagg atcattgaaa tcctttacgg acttccgcat ctgcttgtcg
     3181 ttattctgtt aatggtcatc atgggtccaa gtttgacgac aatcatcgtc gcactgacaa
     3241 tcaccggatg ggtcggaatg gcccggattg tcagggggca agttctgcaa atcaagaact
     3301 atgaatttgt aaccgcctcg aaatcgttcg gagcgaaaac acctaggatc atccggaaga
     3361 acttgctgcc gaatacaatg ggaccgatca ttgtccaaat gacgctgact gtgccgagcg
     3421 ccattttcgc tgaagctttc ctaagcttca tcgggcttgg aatccaggcg cctgttgcga
     3481 gctggggggt catggcgaac gacgcattgc ctgtcatcct gtcaggacat tggtggagat
     3541 tattcttccc ggcgttcttc atttcaatga caatgtttgc atttaacgtc ctcggagacg
     3601 gtatgcaaga tgcacttgat ccgaaactga gggggtgagg gattggaaga gagaaaattt
     3661 gcgccgattg atgaaaacca aggcggaaca gcggttgccc cggcacctaa acgattgcgc
     3721 aagcgaacga aaacggcttc ggaagtcatg ctttccatcc gggatctgca tgtatccttc
     3781 aagacgttcg gtggggaagt gcaggcactc cgcggcgttt cattcgatct gtataaaggg
     3841 gaaacgctcg ccattgtcgg ggagtcggga tgcgggaaaa gcgtcaccgc gaatacgatc
     3901 atggggctca ttccgcaacc ggcggggcgc attaaaaatg gaagtgtcct ttttaaggga
     3961 aaggatttga cgaagctcga taaatccgaa atgaggaaag tgcaaggggt cgacatttca
     4021 atgatcttcc aagatccgat gaccgccttg aacccgacac tcaaaatcgg cgagcagctg
     4081 acggaagggt tgcgtaccca ccaaaaagtg agtaaagccg aagcgcgtga aaaagcgatc
     4141 agattgctgg aagtggtcgg catcccgaat ccggaagaac ggatgaaaca atatccccat
     4201 caattttctg gagggatgag gcagcggatt gtcattgcaa tcgccttaat ctgtgaacct
     4261 gaactgctta tcgcggatga gccgacgact gcattggacg tgacaatcca ggcgcagatt
     4321 cttgaactat tcgatgaaat acaggagcgc acgggcgttt caattattct catcacgcac
     4381 gatttggggg tcgttgcgaa aatcgcggat cgaattgcgg tcatgtacgc agggaaagtc
     4441 attgaaatcg gtgataagcg cgaagtgttc tactcgccgc agcatcctta tacgaaaggg
     4501 ctgctcgcct ccgttccaag gttggatctg aaagaggaag agcttcaacc gattgaaggg
     4561 accccgccag atctattctc tccgccggtc ggatgcccgt tcacggccag atgcccgttc
     4621 gccatggaag tatgcgaacg agtctatccc gctaccaccg gtatatccga tacccatgca
     4681 gtggattgct ggctgcagga tgaaagggcg aagaaagtgt tcggtcataa ctaggttttt
     4741 cgcacgaaag tgcttagcaa gtcatatata aacaaagggg gcttttggaa tgaagaaatg
     4801 gttaacattt ttgatggtgg caatgcttgc gctcgttctt gcggcatgta ccgcaaacaa
     4861 agacgcgggc aaggaacctg aaaaaccggc ggacacgacg ccgacggatg aaggaaaaga
     4921 tgagggtaaa acagacgaaa cggcttcagg ggagaagatc ctcctcatga acaacgggga
     4981 agagccgaca tcgttcgatc cgtccatcgg attcaacgca gtatcctgga gtgcactcaa
     5041 caacatcatg gaaggtctga cacgcctaga taaggaccat aaagcagtag aggctacagc
     5101 agaaaaaatc gacatttccg atgacggatt gacgtataca ttcacaatcc gcgatggcgc
     5161 aaaatggtcg aacggcgatc cggtcgtagc gggcgatttc gtattcgcat ggaagcatat
     5221 gttgaatccg gaaacagctt ctcctgcagc gttccttgca tacttcatcg aaggtgcgga
     5281 agcgtacaat aacggagaag gttccgctga tgacatcgca atcgaagcga aagatgaaaa
     5341 gacgttcgtc gtcaaattga catcaccgaa cgaagcattc ctgaacatca tcacaaaccc
     5401 aagcttcttc ccaatcaacg aaaaagtggc gtctgccgat ccgacttggc atacggaagc
     5461 tgcaacattc gtagggaacg gaccattcaa gttgtctgct tggagccacg atgtcagctt
     5521 cgaatttgaa aagaacgaac attattggga tgctgacgca gtcaagttgg ataaagtcca
     5581 ctgggcaatg gtgaacgact ccacgactgc atatcaaatg tatcaagcca atgaattaga
     5641 tgtaactggg gtaccttcag aaatcgctga tcaattgaaa gatagcccgg aattacgcat
     5701 cgatgaccaa gcgggcttgt atttctaccg cttcaatact tcgatggaac cattcacgaa
     5761 caaaaagatc cgccaagcat tcgcatttgc ggtcgatcaa gaggaaatcg tcaatttcgt
     5821 aacgaaaaac ggtgagaagc cggctcatgg cttcgtcaca tacggcttca tcggaccgga
     5881 tggcaaggaa tttcgtgata gtgtaggcga tctcgtttcc ttcgatccgg aaaaagcgaa
     5941 acagcttttg gcggaaggga tgaaagagga aggctattcg gagcttccga aagtgacaat
     6001 cacctattca acgagcgaat cgcatcagaa catcgcgatt gcacttcaat ccaaattcaa
     6061 agaggtgcta ggtgttgatg cagagctcca aaacgtggaa agcggagttt tcctttccga
     6121 gcaaaaagag ttcaaatacc aaatgtcccg ttcctctttc ctgcatgact atgcagatcc
     6181 tgtgaacgcg cttgaaagct tcatcaccgg ctcttccatg aaccgtacaa catggtcgaa
     6241 cgctgaatac gataagctga ttgcggacat taaaaatgaa acggacgaaa acaaacgctg
     6301 ggaattgctg aaggaggcag ataaacttct aatggatgaa atgcctgtat ttccactata
     6361 cttctacaac tcgacatcac ttgaaaaaga aggcgtttcc ggcattctgc gccaccctgt
     6421 cggctacgtc gacttgaaat acgcagacaa gaactaatgc acaagtcgcg gtgtccgtca
     6481 tggcgccgcg actttccaat tttgcgtgaa acggcgaatt tatgcgtgaa taaaatgatt
     6541 tatgcgtgaa tccagcggtt tatgcgcgga ttcatagatt tatgcgtatt tgcaaacgtt
     6601 tatgcgtgaa tatagggatt tatgcgtttt tagatgaagt tatgcgtcga agactatgat
     6661 ttttcattga aagaggtgca ggagattgag aatacgaccg aaaaggcttc agccgggcga
     6721 tacaatcgga atcgtcgcgc cgtcgagccc gccgaatcaa gaaagcttgg agcgttcgct
     6781 cgcttttctg gaacagctcg gcctgaaatg gaaattcgga caatatgtga aaaatgtcaa
     6841 tggctatttg gcggggacgg atgatgaacg gctgcaggac ttggaggaca tgttcactga
     6901 tccgtgcatc agcggcatca tttgcgctgg cggaggttac ggttctgcac gctataccga
     6961 ccggctcgat ttgcagctga tccgggaaaa tccgaaagtc ttctggggat tcagtgacat
     7021 cacattcctc catacagcga tcggtcttta ttcggatctt gtcaccttcc atggcccgat
     7081 gctcgcttcg tgtgtcggaa aggatacttt ccatgaacta tccgcaaaaa tgttccaaca
     7141 attattcgaa ccgaaggagc tgcattatac ggaggagatt tcaccgttga gggcaattac
     7201 cgggggaagc gcacatggcg agcttgtcgg aggcaatttg tcgctccttg cggatacggt
     7261 cggaacgaaa ttcgaaatcg atacgaaggg caagctgctg ttcatcgaag atgtcggcga
     7321 ggagccttat cgggtcgatg ggatgctgaa ccagctgcgc atggcaggga aacttgcaga
     7381 cgctgcgggc atcgtcatcg gggatttctc gaatgttgag ccgaagaagc aacagaggac
     7441 cttgtcattg gatgaagtat tccgccatta tttgggcggc ttggagaagc cggttgtgga
     7501 gggcttcaag ataggccact gcgagccgca tttcgcgatt ccgctaggag tggacgcgaa
     7561 gctggacgca gatcaaaaga cattgacgat tttgccgggg gtggaatgaa tgcgcatccg
     7621 ttcaatcgaa acaattcccg tcgccgttcc gctgacaaag ccgtttaaaa cggctctccg
     7681 gacagtgacg acagcctatg cgatctacgt gaaaataaca agtgaagacg gacggaccgg
     7741 gtggggggaa gcacccccga cccatgtcat cacgggcgac tccctcggca gcattacata
     7801 cgcgattgaa gaggtgatcg ctccgcagtt gatagggaag gatttgcgga atcgtgaaga
     7861 agtcttccga ctgctgaaac ggtcgatcgt ccgcaataca agcgcgaaag ccgccatcga
     7921 catggccatc catgatctgc ttggccagtt ggcgggactt ccgctctgtc aactgctcgg
     7981 cggattccgg gagaaaatcg aaaccgactt cacagtaagt gtcaatgatg ccgctgaaat
     8041 ggcggatgac gcggaacgtt atattgctga cggcttcaat gttctgaaag tgaaagtcgg
     8101 catcggggat attagcgagg atatcgaacg tatcaaggcg atccggggaa gggtcggtaa
     8161 gggtccaaaa atccggcttg atgcgaacca agggtggaag cccaaggaag cggtttatgc
     8221 gattggcaag atggaggacg ccggtcttgc tattgaattg attgaacagc cggtttctgc
     8281 ggatgatatc gacgggcttc agtatgtgac gaatcaaacg ctgacaccga tcatggcgga
     8341 tgaaagcgtg ttttccccga aagacgccct ccgtgttttg gaacggaggg cagcggattt
     8401 gattaacatc aagctcatga aatctggagg gattcacgag gcattgaaaa tcaatgcatt
     8461 ggccgaaacg tatggcgttg aatgcatggt cggcagcatg atcgagacga agctcgggat
     8521 tacggctgca gcgcatttcg ctgcaagcca gccgaacatt acaagattcg atttcgatgc
     8581 gccccttatg ttggcagagg acattgtcgc cggcggaatc acttatgagg gtagggtcat
     8641 ccggctgcca gaagggccgg ggctcggatt ggatcgggaa ggaatgggat taatcggcaa
     8701 ggaggcaggg aaatgacgga gttgacagtg aaaatgtata catgcgcagt gccggttgcg
     8761 acggtatgga cttcaccggc atctgtgcgg gagatggata cggacggtgt atccaacccg
     8821 attcatctga ataaatggct ggagaagctt gcatatgagg agcggctgga tctttgcaat
     8881 ggcaatcggg tgcagacgca attgttgtat ggcgaaccgg tcatcgtcga cgaaattgaa
     8941 ggtgattggg cgaaggtcat tgcggtatgg cagccatcga agaaggatga acgaggttat
     9001 ccggggtggg tgccagtacg tcaattgaaa aaggcgtccc cgttgaagga agcgggcgga
     9061 tttgtaagag tggcagttgc gaaatcgcaa ttatggaaac cggatgggac gccgctcatg
     9121 attgtcccat tcaatacaat cctaccgctg accgatgaaa gcgacaatca cttccatgtg
     9181 gaaacgcctg acggggaggc attgttgctg aaaacggata ttgtgcgggc gccatccgtc
     9241 catcaattca agaagcggaa agcagatgca gccgttgaag agggactcgc ctatttggat
     9301 ctcccgtatt tctggggggg catgtcatcg tatggctttg attgctcagg cttcacgtac
     9361 aatatgctga aagcttgcgg acatttcatt ccgcgggatg ccggcgatca ggcgaaaagc
     9421 ggcgaggaag taccgatggg taagccggaa cggtgggaaa agggagatct tctattcttt
     9481 gcgaacgatg aaggaaaagg c
//
LOCUS       DASULI010000070         7933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_985730, whole
            genome shotgun sequence.
ACCESSION   DASULI010000070 DASULI010000000
VERSION     DASULI010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985730"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFX34_01615"
     CDS             complement(<1..680)
                     /locus_tag="VFX34_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX5563630.1"
                     /translation="MKALQVVTGIVSKFLPIWIVLLSVIAYLAPGFFTPIRSMTGAGL
                     GTIFLLMGLSLSTDKLMEVIKNPKFAFAGVFLKWTMTVGVSIAIAYLFFKDEAEIATG
                     VILAGTVPSGTSANIYTFIAGGEVALSITMATMDTFISPVLTPTLVQVFAGKLIPIAF
                     WPLFLNIIYIVFIPLLAGLFIQWKWTKRVQAIKPYTSVLSQLALFIVILSVISSAQQS
                     LQQNLSVLP"
     gene            complement(746..1051)
                     /locus_tag="VFX34_01620"
     CDS             complement(746..1051)
                     /locus_tag="VFX34_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5563631.1"
                     /translation="MKIYTCNSCDYASVSSKYTCPACGTGKLIAAEVSSKGTVFSFTD
                     IHIAPAEFADIAPYTIALIQLDDADVKVTARMDGPVEIGDKVDLQKIEDGAYLYTKI"
     gene            complement(1056..2189)
                     /locus_tag="VFX34_01625"
     CDS             complement(1056..2189)
                     /locus_tag="VFX34_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX5563632.1"
                     /translation="MTNVYVHGIGMTQFGTFVDRSMKELLMDACIQALEDAGNPKVDA
                     VFVGNFMGGAIYNQEILGAIVANELGLGFIPTAKVEGACASGGIALRQGIMGILSGEY
                     ETVLVAGVEKMKHASTTDVTQAINAAMDNDSNEKQAGLTFPGFFGVLANRYFYETGAS
                     KKHLAMVALKNREHALNNPLAQFQKPATLDEIMNARIITNPLGLFDCSPMTDGAAALI
                     ISKKKSGVHIRSSTQSSGPTQMQDADDLLSIPAIRESGRLAYEKAGVGPEDIDVVEIH
                     DCFSMTEVLAIEELGFFNKREGWKAVEEGRTKSTGDKPVNTSGGLLSRGHPIGATGIA
                     QIYQLVRQLRGTAPNQVDGARLALAQNLGGTGSYSTVHILERV"
     gene            complement(2186..3667)
                     /locus_tag="VFX34_01630"
     CDS             complement(2186..3667)
                     /locus_tag="VFX34_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5563633.1"
                     /translation="METLGKLAMKSAAAYPDKAAVKDKDRSFTYREMMERVFALTAYF
                     HETGLHKGDRVGILMANRLEHIELDVAASLAGVIKVPLNYRLHPKEHEYMLKDADVSL
                     IIGDEGLIEPIGTDVAVLTIGEEYEQAVASHIGKRHMEEVGEDDLFAIMYTSGTTGNP
                     KGVMLSHRNMISGALSLAVACDTNYDDIIGHVAPLTHGSNFLSHVAWLYGLTQVVFDK
                     FEPEEFVNDLARERVSTIFLVPTMVNLMIQHPNFDPAKLSTLKTINMAGSAIAASKLE
                     QALTLAGPIFAQTYGQVEAPMCITMMPKKEMGNRLESCGRTGLFVDVKIVDDEGNEVA
                     DGEVGEITCKGSLVMQGYWNNKQATEETLKDGWLHTGDLGWKSAEGYLHIVDRKKDVI
                     ISGGVNIYPREVEEVLNLHTGVKETCVIGVPDEKWGENVVAYVVLNGTAAVTEQELIA
                     LCVENMASFKKPKEIHIVDELPKSSYGKILKRELRSKHEEVKA"
     gene            complement(3673..4146)
                     /locus_tag="VFX34_01635"
     CDS             complement(3673..4146)
                     /locus_tag="VFX34_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEX5563634.1"
                     /translation="MDKRIQAVHDRKSDRIAGRLMKGIDLFEGIKGVCKGFGVEAAQF
                     QCMGSLEHATYVQLNRNEDDTLQYSAETVTDTPVELLSGTGFVGLDMEGKPEVHFHGM
                     FVDCNKNLNGGHFIDGENPVAVTVEFVIFPLNNIRMQRGLDEIYGIPVFQFREKE"
     gene            complement(4139..4897)
                     /locus_tag="VFX34_01640"
     CDS             complement(4139..4897)
                     /locus_tag="VFX34_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5563635.1"
                     /translation="MKLKGQTAIITGAASGIGAETAIRLAREGANIVLIDLNPCTKTI
                     DSIQGENPDANVLECLGDVRDPEFVKAAIARASDSFGELHILVNNAGTAGRLGIDNTT
                     LEIWDRDMDTNVKATFLFIQNVVYPHMVDQKYGRIINISSISGLNGGVTSGDGANGRS
                     GPAYAASKGAVIALTKWAAKELGVLGITCNSVAPGATETGITSGVPYDVSQQAIKRMG
                     TPADIAEAVLYFALPESGYTTGQVLKVDGGYSFG"
     gene            complement(4910..6229)
                     /locus_tag="VFX34_01645"
     CDS             complement(4910..6229)
                     /locus_tag="VFX34_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5563636.1"
                     /translation="MMAFILFGLFFLLVMLRLPIAVALAISSIAVLFTGSGMFGLELV
                     ADIMYSSVSKFTLLAIPFFILAGVIMEQAGISKRLIDFAQSLVGHRKSGIVFVTVMTA
                     IFFAAISGSGPATVAAIGGILIPAMVKNGYRKETAGGLVASSGAIGIIIPPSIAFIVF
                     AVVAGDQIPVSIGRLFIAGIIPGLLLGGGFILAAMYVRWKQERTEGPIEGHVQEKATF
                     AERVKSFISAFWGLLIPIIILGGIYGGFFTPTEAAVVAVFYGLFVGFVIYRELNLKKL
                     YDILVAASVQTATVMFIVSAASVYAYIITTEQIARQISGAMLGISENPIIILLLVNVL
                     LLIAGMFIDAISAYYIFIPILLPIMLVMNVDPTVFGVFMTVNLAIGLFTPPVGLNLFV
                     AAGISGTSIGRLSVGVMPFIVSAIIILLLITFIPQLSTFLPDLLNVK"
     gene            complement(6226..6711)
                     /locus_tag="VFX34_01650"
     CDS             complement(6226..6711)
                     /locus_tag="VFX34_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5563637.1"
                     /translation="MERVISRLEEWIVVIVLSIMSTIAFVNILSRGFANHSFSFAEEI
                     TVNLFVALTFVGTAIGVRQYAHLGFTLIYDTANLFVKNIITILVGVLMSLLFVILIYF
                     GIQMLMFQVQMGQKTPALGWPQWIFSMAMPIGAALCLYRTVQATIKEFREQNGKGEKA
                     V"
     gene            complement(6727..7746)
                     /locus_tag="VFX34_01655"
     CDS             complement(6727..7746)
                     /locus_tag="VFX34_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5563638.1"
                     /translation="MKLKKGRILGAIGLTAMLMLGACSKDDTGSAASDKKYNLKMSVT
                     VNDSSTWYKAAEKLANDMKEETDGRINIEVFPNEQLSGGDSGKGVELLAKGQTDMSFH
                     STIIYSILDDRFGVASAPFLFSDHEGVDKVFKGAGGEKIGEILNEKGVEVLGFGENGF
                     RQITNSKHEIKSPADMKSLKIRIPGINMYTDLYRALGADPTTMAFSEVFTSLQQGTIH
                     GQENPIDVIHSSKLNEVQDYLTEWNYSYDPLVLGINKKLFDSMSKEDQELFKKLGKEA
                     ADYQIGLAREKEAKQIEELKAAGMQFYEPTEAEMEEFKAAVQPLYEKYKDIWGEDLLK
                     AFQDQ"
     gene            complement(7748..>7933)
                     /locus_tag="VFX34_01660"
     CDS             complement(7748..>7933)
                     /locus_tag="VFX34_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase"
                     /protein_id="HEX5563639.1"
                     /translation="ILGSKMEEVKQVQATTIITTNPGCLLQMKVGVEREGLSGTARAM
                     HVVDFIHEKIMESNGGK"
BASE COUNT         1910 a         2222 c         1762 g         2039 t
ORIGIN      
        1 gggagtacgg acagattctg ctgcaatgat tgctgagcac ttgagatgac cgacaggatg
       61 acaatgaaca acgcgagctg ggagaggacg gatgtgtacg gcttgatggc ctgcaccctt
      121 ttcgtccatt tccattggat gaacaatcct gccagcaatg gaatgaacac aatataaatg
      181 atattcagga acagcggcca aaacgcaatc ggtattaatt tccccgcaaa cacctgcacg
      241 agcgtcggtg tcaaaacagg cgatatgaac gtatccattg tcgccattgt gatgcttaaa
      301 gccacttcgc cacctgcgat gaatgtgtag atgttggcgg aagtgccgct tggtaccgtc
      361 cctgccaata tgacgcccgt cgctatttcc gcttcatctt tgaagaagag atacgcaatc
      421 gctattgaaa cgccgactgt catcgtccat ttcaggaaga cgccggcaaa ggcgaatttc
      481 gggtttttaa tgacctccat caatttatcc gtcgacaatg acagtcccat aagaagaaag
      541 atcgttccga gcccggcacc tgtcatgctg cgtatcggag taaaaaaacc gggggccaag
      601 tacgcgatca cggatagaag gacaatccat atcggcaaaa atttcgatac gattcctgtc
      661 accacttgca atgctttcaa cggatctccc cctttacaaa aagacctgca cctggaatca
      721 tcacaccgat tccaggtaca agccctcata ttttcgtata taaataagcg ccgtcctcga
      781 tcttttgcaa atcaaccttg tctccaatct caaccgggcc atccatcctt gcggtcacct
      841 tgacatccgc atcgtccagc tggatgagcg cgatcgtgta aggggcaatg tctgcaaact
      901 cggccggagc gatatggata tcggtaaaac tgaaaaccgt ccctttcgag gacacttcag
      961 cagctatcag cttgccggtt ccgcacgccg ggcatgtgta tttcgatgag acgctcgcgt
     1021 aatcgcatga attgcatgta taaatcttca tgtcctcaca ccctctccaa aatgtgcacg
     1081 gttgaatacg aacccgtgcc gcccaagttc tgggccaatg ccagccttgc cccgtccact
     1141 tggttcggcg ccgtgcccct cagctgccgg acgagttgat aaatctgggc gatccccgtc
     1201 gccccgatcg gatggcctcg tgacagcaag cctccgctcg tattgaccgg tttatcccct
     1261 gtcgacttcg tccgtccttc ttcgacagcc ttccaccctt ctcgtttatt gaagaagccc
     1321 aattcctcga tggcgagcac ttccgtcatc gagaagcagt catggatttc gacgacatcg
     1381 atatcttctg gaccgacgcc agccttttca taagcaaggc ggcccgattc acgaatcgcc
     1441 ggaatggaca acagatcatc cgcatcttgc atttgggtcg gtcccgagct ttgggtggat
     1501 gaacggatat gcacacctga tttcttcttt gaaataatga gtgccgccgc tccatccgtc
     1561 atcggtgagc agtcgaacag gcctaacgga ttcgtgatga tgcgggcgtt catgatttca
     1621 tcaagcgtcg ccggcttttg gaattgggca agcggattat tgagcgcatg ttcgcggttc
     1681 ttcaacgcga ccatcgccaa atgttttttc gacgcgcctg tttcgtagaa ataacggttt
     1741 gccaacacgc cgaaaaaccc cgggaatgtg agccctgcct gcttttcatt cgaatcattg
     1801 tccatcgccg cattgattgc ctgggtgacg tccgttgtgg acgcatgctt catcttttcc
     1861 acgcctgcaa caaggaccgt ttcatattcc ccgctcaaga tgcccataat gccttgccgc
     1921 aatgcaattc cgcctgaagc gcacgcccct tcgaccttcg ctgtcggaat gaaaccaagt
     1981 ccaagctcat ttgcgacaat cgccccgagg atttcctggt tatagatggc gcctcccatg
     2041 aagttcccga caaatacagc atcgactttc ggattgccgg cgtcctccaa tgcttggata
     2101 cacgcatcca taagcagttc cttcattgac cggtcgacga acgtcccgaa ctgtgtcatt
     2161 ccgattccgt ggacatatac atttgtcatg ccttgacctc ctcatgtttg ctgcggagtt
     2221 cccgtttcag gatttttccg tatgagcttt tcggcagttc atccacgata tgaatctcct
     2281 tcggcttttt gaagcttgcc atgttttcga cgcacaaagc gatgagttcc tgttccgtca
     2341 ctgcagccgt tccattcagg acgacatacg cgacgacatt ctcgccccac ttctcgtcag
     2401 gaacgccgat gacgcacgtc tccttcacgc cggtatgcaa attgagcact tcctccactt
     2461 cacgcggata aatattgacg cctcccgaaa tgatgacatc ctttttgcgg tcgacgatat
     2521 gaagatatcc ttcagcactc ttccaaccga ggtctcccgt atgaagccag ccgtccttca
     2581 acgtctcttc ggtcgcctgc ttattattcc aatagccttg catgacgagg gatcctttgc
     2641 aggtgatttc gcccacttca ccgtcggcaa cttcattccc ttcatcatcg acgattttga
     2701 cgtcgacgaa aaggcctgtc cgcccgcatg attccagcct attccccatt tccttcttcg
     2761 gcatcattgt aatgcacatc ggcgcttcga cttgcccgta tgtctgggcg aatatcggtc
     2821 cggcgagcgt caatgcctgc tcaagcttgc ttgctgcaat tgccgagcct gccatattga
     2881 ttgtcttcaa cgtcgacagc ttcgctggat cgaaattcgg atgttggatc atcaagttca
     2941 ccatcgtcgg cacaaggaat atcgtcgata cccgctccct cgccaaatca ttgacgaatt
     3001 cctccggttc gaatttatcg aacacgacct gcgtcaagcc atataaccag gcgacatgtg
     3061 aaagaaagtt gctgccatgt gtcagaggcg cgacatgtcc gataatatcg tcgtaattcg
     3121 tatcgcaagc gacggcgagc gacaaagcac ccgatatcat attgcggtgg gatagcatga
     3181 cgcccttcgg attgcccgtc gttcctgatg tgtacatgat cgcgaacaga tcgtcttccc
     3241 caacttcctc catatggcgc ttgccgatat gtgaagcaac tgcctgctcg tactcctccc
     3301 cgattgtcaa tactgcgaca tccgtcccga tcggttcaat caacccttca tccccgataa
     3361 tgaggctcac atcggcatct ttcagcatat attcatgctc cttcggatga aggcgataat
     3421 tcaatgggac tttaatgacg ccggcgagcg aagcagcgac atcaagttcg atatgctcga
     3481 gccggttcgc catgaggatg ccgacccggt cgcctttgtg cagtcctgtt tcatggaaat
     3541 atgcggtcag ggcaaaaacc cgttccatca tttcccggta tgtaaatgac cgatccttgt
     3601 ctttcacggc cgccttgtcc ggataggcgg cagccgattt cattgccaat ttccccaatg
     3661 tttccatgcc cctcactcct tctccctaaa ttgaaataca ggaatgccgt aaatctcatc
     3721 caatccccgc tgcatccgga tattgttcaa tggaaaaatt acgaattcca ccgtgacggc
     3781 gacaggattt tcaccatcaa tgaaatgccc gccgttcagg ttcttattgc aatcgacgaa
     3841 catcccgtgg aaatggactt ccggcttccc ctccatatca aggccgacaa atccagtccc
     3901 cgacagcagt tcaaccggcg tatccgttac cgtttccgct gaatattgca gtgtgtcatc
     3961 ttcatttcga ttcaattgga catacgttgc atgctcaagc gatcccatgc attgaaactg
     4021 ggccgcttct actccaaacc ccttgcagac acctttaatc ccttcaaata gatcgatgcc
     4081 tttcatgagg cgtccggcaa tccggtcaga ctttctgtcg tgcacagctt gaatacgctt
     4141 atccaaaact gtagccccca tcgaccttca acacctggcc ggtcgtatag ccggattccg
     4201 gcaatgcaaa atagaggacc gcttcagcga tatcggcggg tgtgcccatc cgtttgatcg
     4261 cttgctggct gacgtcataa gggactccgg aagtgatgcc cgtttcggtt gctcccggtg
     4321 ccactgaatt acaagtgata ccaagcacgc ccaattcctt tgccgcccat ttcgtcaagg
     4381 cgatgaccgc ccctttggaa gcagcataag ccgggccgga tcgaccattc gcgccatctc
     4441 ccgaagtgac gccgccgttc agtcctgaga tggaactgat gttaatgatc cgtccgtatt
     4501 tctggtcgac catatgcgga tagacgacat tttggatgaa taggaaagtg gctttcacat
     4561 tcgtatccat atccctgtcc catatctcca gcgtagtatt gtcaattcca agtcttccgg
     4621 cagtccctgc attgttcacc aaaatatgca gttccccgaa tgaatcggag gcacgcgcga
     4681 tggctgcctt gacaaattcc ggatcacgga cgtccccgag gcattccaat acgtttgcgt
     4741 cgggattctc cccttggatg gaatcaatcg tttttgtaca aggattcaag tcgataagaa
     4801 caatattcgc gccttccctg gcaagacgga tagcagtttc ggcaccgatg ccgctagccg
     4861 ctcctgtgat gattgctgtc tgccccttaa gtttcatgcc aatctccccc tatttgacgt
     4921 ttaataaatc tggtaaaaat gttgataatt gcggtatgaa cgttatgagc agcaggatga
     4981 tgattgcgga aacgatgaat ggcataaccc ctaccgacaa cctgccgatc gatgttcccg
     5041 aaatgcctgc cgccacgaac aagttcaatc ctaccggcgg tgtgaaaaga ccgatagcca
     5101 agttgactgt catgaaaaca ccgaaaacgg tcgggtccac attcatgaca agcatgatcg
     5161 gcaataaaat cggaatgaaa atatagtagg cggaaattgc atcaatgaac attcctgcaa
     5221 tcaacagcaa gacgttgaca agcaatagga tgatgatcgg attttcagaa atgccgagca
     5281 tcgcaccgga aatctgtctt gcaatctgct ctgtcgttat gatatatgcg tagacggatg
     5341 ccgcactgac gatgaacatg actgtcgcgg tctggactga tgccgccacc aatatgtcat
     5401 acagcttttt caaattcagt tcgcggtaaa tgacgaagcc gacaaacagt ccgtagaaga
     5461 ctgcgacaac cgccgcttca gtcggtgtga agaacccgcc ataaattccg cctaaaatga
     5521 tgattggaat caataatccc caaaatgcac tgataaacga tttcacgcgc tccgcgaatg
     5581 tagccttctc ttggacatgg ccttcaatag ggccttccgt gcgctcctgt ttccatctga
     5641 catacatcgc agcaagaatg aaacccccgc caagcaataa tcccgggatg atgcccgcga
     5701 tgaacagtct tccgatggat acgggaatct gatcgccggc aacgaccgcg aatacgatga
     5761 aggcgatgct cggaggaatg ataatgccga ttgcacctga acttgctacg agacccccag
     5821 ccgtctcctt cctatatccg ttcttcacca tagccggaat gaggatccca ccgatcgccg
     5881 cgacagtcgc cgggcccgat cccgaaattg ccgcgaagaa gatcgctgtc atgacagtga
     5941 caaaaacgat tccgcttttc cgatgcccga ccaaagattg cgcaaaatcg atcagccttt
     6001 ttgaaattcc ggcctgttcc atgatgaccc ccgccaaaat gaaaaacggg atggcaagca
     6061 atgtgaactt cgacacactc gaatacatga tgtcggcaac caattcaagg ccgaacatcc
     6121 cgctgcccgt gaacagcacc gcaatggacg agatggcaag cgcaaccgca atcggcagcc
     6181 gaagcatgac aagtaagaaa aacagtccaa ataagatgaa tgccatcata ccgccttctc
     6241 ccctttcccg ttttgctcac ggaactcctt gattgtcgct tgcactgtcc tgtacagaca
     6301 taaagccgcc ccgattggca tcgccattga gaagatccat tgcggccatc cgagtgcagg
     6361 ggttttctga cccatttgca cttggaacat cagcatttga atcccgaaat agatgagaat
     6421 gacaaataaa agcgacatca ggacgccaac gagaatcgtg atgatgtttt taacgaataa
     6481 attcgccgtg tcgtaaatca acgtgaagcc aaggtgtgcg tattggcgca caccgatggc
     6541 cgtcccgacg aaagtcagtg cgacgaacag attcacggtg atctcctcgg caaaggagaa
     6601 cgaatggttc gcgaatcccc tcgacaggat attgacaaaa gcgattgtgg acatgatgga
     6661 caaaacgatt acaacaatcc actcttctaa tctgctaatc actctttcca ttcatgttcc
     6721 ctccacttat tgatcttgga acgctttcag caaatcttcg ccccagatgt ctttgtattt
     6781 ttcatatagc ggttgaacgg cggctttgaa ttcttccatt tccgcttcag tcggctcata
     6841 gaattgcatg ccggcagctt tcaactcttc aatttgcttc gcttcttttt cacgtgcaag
     6901 cccgatttgg taatctgcag cttctttccc aagtttcttg aacagctcct ggtcttcctt
     6961 gctcatcgaa tcgaagagct ttttattgat gccgagcacc agcgggtcat acgagtagtt
     7021 ccattccgtc aaataatcct ggacttcatt cagctttgat gaatggatga cgtcaatcgg
     7081 attttcctgt ccatggatcg tgccttgctg caatgacgtg aacacttcag agaacgccat
     7141 tgtcgtaggg tcggcaccaa gtgcgcgata tagatccgtg tacatgttta tgccaggaat
     7201 acggattttc aagcttttca tatccgcagg cgatttaatt tcatgcttgc tgttcgtaat
     7261 ttggcggaag ccgttttctc cgaatccgag cacttcaacg cctttttcat tcaggatttc
     7321 cccgattttt tccccgccgg cgcctttgaa tactttatcc acaccttcat ggtcactgaa
     7381 caggaatggt gcactcgcca ccccgaaacg atcgtcaagg attgaataga tgattgtgga
     7441 gtggaagctc atgtccgtct gcccctttgc cagcagctca acgccctttc ctgagtcccc
     7501 gcctgataat tgctcattcg ggaacacttc gatattgatc cttccatccg tctcttcttt
     7561 catgtcattg gcgagtttct ccgcggcttt gtaccatgtt gaggaatcgt tcaccgtcac
     7621 ggacatcttc aaattgtatt ttttatcact tgcagcactg cccgtatcat cttttgaaca
     7681 cgctccaagc atgagcattg ccgttaaccc gattgcccct aaaatcctgc cttttttcaa
     7741 tttcatttca tttccctcca ttggattcca ttattttttc gtggataaaa tcaacaacat
     7801 gcatggcgcg ggcagttccg gataagcctt ccctttccac accgactttc atttgaagca
     7861 aacatcctgg attcgtcgta atgatcgtcg ttgcctgtac ctgtttcacc tcttccattt
     7921 tcgaaccgag aat
//
LOCUS       DASULI010000071         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1465309,
            whole genome shotgun sequence.
ACCESSION   DASULI010000071 DASULI010000000
VERSION     DASULI010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465309"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFX34_01665"
     CDS             complement(<1..825)
                     /locus_tag="VFX34_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5563640.1"
                     /translation="MQLHDFEKAVLDGDIGLVNDLIDRAENSGEFDLLYEAAGLLVDY
                     GYIGQADHIYETLISYMPDEPQLKIDRANTLMELGDEDEALLLLSEITPNDEEYVQAL
                     LALADYYQMIGMAEAAIGKVKDALARAPHEPVIRFAYAELLLDAGRYLEAAKLYSELY
                     NEGHKEIAGAMITLRLAETYSAGAAYEDALPYYEELLKEEHLPDTLFGAAFAYYQSNH
                     PEKSIHLLEELIGMDPDYFSAYMLLGQAQSQLGNDDKAYEYFEQGIKRDEFDKELRL"
     gene            complement(868..2175)
                     /gene="aroA"
                     /locus_tag="VFX34_01670"
     CDS             complement(868..2175)
                     /gene="aroA"
                     /locus_tag="VFX34_01670"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5563641.1"
                     /translation="MVEMKTVKFNKPFLKGSLKVPGDKSISHRAIMLGSIAKGRTAIK
                     GFLDGEDCLRTIEIFKKLGVSIERNGTDVLIDSPGMDNWKTPTEELYAGNSGTTARLM
                     LGILAGSAVTSTLTGDESLSRRPMKRVTTPLASMGASVSGNEGGEFLPLTIVGSKLEG
                     IDYQMPVASAQVKSAVLFAGLNAIGETVVRETTVSRDHTEQMLKQFGAKLEQEGNTVR
                     IQGGQALEGTEVTVPGDISSAAFFMAAAAMVEDSEVEFVDVGLNPTRTGIIQVLRQMG
                     AGVKILEKHGETGEPYGVVKVTHARLVGTEISGDIIPTLIDELPIIALMATQAAGKTI
                     IKDAAELRVKETDRIAAVTDELTKLGAKVEATEDGMIIEGPTPLSGGQLKSYGDHRIG
                     MMAAVAALVSDGPIHIEDPSCISISYPSFFEHLSQLVTAGIEE"
     gene            complement(2191..>2517)
                     /locus_tag="VFX34_01675"
     CDS             complement(2191..>2517)
                     /locus_tag="VFX34_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5563642.1"
                     /translation="ENDPDRIYGFFSGAKEFRDKLPSTFQGAVQGALYMTFDLHIDIP
                     DHPGVISEITKILADEQISITNIRIVETRTDVYGILVISFQTADDRKSARAILAKTTD
                     YSMHIL"
BASE COUNT          606 a          655 c          518 g          738 t
ORIGIN      
        1 caggcgtagt tctttgtcaa attcatcacg cttgatgcct tgctcgaaat actcatatgc
       61 tttatcatca tttcccaatt gggactgtgc ctggcctaag agcatatagg cagagaaata
      121 atcgggatcc atcccgatca attcctcgag caaatggatc gatttttccg gatgattgct
      181 ttggtaatag gcaaatgcgg caccaaatag tgtatcagga agatgctcct cttttaaaag
      241 ttcctcatag taagggagcg catcctcata agccgcaccg gcactgtacg tttctgccag
      301 gcgtagcgta atcatcgcac ctgcgatttc cttatggcct tcattgtaaa gttcggagta
      361 aagtttcgcg gcttctagat atcttccggc atctagtaaa agttcagcgt acgcgaaacg
      421 gatgaccggc tcatgcggag cccgtgcaag ggcatctttc acctttccta ttgctgcttc
      481 agccatgcca atcatctgat aatagtcggc tagggctaac agagcctgga catattcctc
      541 atcgttaggc gtaatttcgg aaagcaggag caacgcttca tcctcatcgc ccagctccat
      601 taatgtattt gcccggtcga tctttagttg cggctcatca ggcatgtagg atatcaacgt
      661 ttcataaatg tgatcagctt gaccgatata tccataatcg accaataaac ccgctgcttc
      721 atagagcaga tcgaattcac cggaattttc cgcgcgatca atcaagtcat tgaccaaccc
      781 gatatcgccg tcgagtaccg ctttttcaaa atcatgtaac tgcatcatat tttcacctgt
      841 tccttcatgt tgtatacatt aggaaaatta ttcttcaatg cccgccgtaa cgagttgtga
      901 caagtgctca aaaaaactag gataggaaat ggatatgcat gaagggtctt caatgtggat
      961 gggaccatcc gataccaatg cggctacggc agccatcatt cctattctgt ggtcaccgta
     1021 tgatttcagc tgtcctcccg ataatggggt cggcccttct ataatcatcc catcttctgt
     1081 cgcttccact tttgcgccaa gttttgtcag ctcatctgtt acggcagcaa tacggtccgt
     1141 ttccttgacg cggagttccg ctgcatcctt aatgattgtc tttccagccg cttgggttgc
     1201 cattaatgca atgatcggca attcatcgat aagcgtcgga atgatatccc cgcttatctc
     1261 tgttcctacc agccgggcat gcgttacttt gactactcca taaggttcgc ctgtttcacc
     1321 atgtttttca agtattttaa ctccggctcc catttgtcgc aacacttgaa taattcctgt
     1381 tctcgtcggg ttcagcccga catctacaaa ttccacttcg ctatcttcga ccatcgctgc
     1441 agccgccatg aaaaatgccg cggaggaaat atctccggga actgtgactt ctgtgccctc
     1501 taatgcctga ccaccttgga tccttactgt attcccttcc tgctcaagct ttgcaccaaa
     1561 ctgtttgagc atttgttccg tatggtccct tgaaacagta gtttccctaa caaccgtctc
     1621 gccgatagcg ttcaatcccg cgaaaaggac cgcagatttc acttgtgcac ttgccactgg
     1681 catttgataa tcgattcctt caagcttaga gccgacgatg gtaagaggaa ggaattcccc
     1741 tccctcattc cctgaaacag acgctcccat tgaagctaaa ggtgtggtca ctcttttcat
     1801 cggacgtctt gataaagatt catcacccgt caatgtggag gtgactgctg aaccggctaa
     1861 aattccgagc atcagtcttg ctgttgtacc tgaatttcca gcatacagct cttccgtagg
     1921 ggtcttccaa ttgtccatcc ccgggctatc aattaaaaca tcagttccat ttcgctcgat
     1981 tgatacgcct agcttcttga atatctcgat tgtcctcaag caatcttccc catcaaggaa
     2041 accttttatt gcggtacgac cttttgcaat tgagcctaac atgatcgcac ggtgggatat
     2101 ggatttgtct cccggcactt tcagtgaacc tttcaggaac ggtttgttga atttcactgt
     2161 tttcatttca accatgacga gtccccctta ttagagaata tgcatgctgt aatccgttgt
     2221 ttttgcaaga atcgctcttg ccgacttccg gtcgtcagcc gtctggaaac tgatgactag
     2281 gattccgtac acatccgtac gggtttccac aattcgtata ttcgttatgc tgatttgttc
     2341 atcagcaagt attttcgtaa tttctgaaat gacccccgga tgatccggga tatcgatatg
     2401 caaatcgaaa gtcatataca aagccccctg taccgcccct tggaaagtag aaggcaattt
     2461 gtcccggaat tccttcgctc cggaaaagaa cccgtatatc cgatcaggat cgttttc
//
LOCUS       DASULI010000072         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1037912,
            whole genome shotgun sequence.
ACCESSION   DASULI010000072 DASULI010000000
VERSION     DASULI010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037912"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VFX34_01680"
     CDS             <1..794
                     /locus_tag="VFX34_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563643.1"
                     /translation="GGNGTSLLGLEGELRFENVSFRYPHTDGEEGILKNVSFHVAPGE
                     KLAIMGATGSGKSTLLNLIPRMFDATEGKIYVGGKDVQEWPLKNLREAIGYVPQQSIL
                     FTGSILENLSWGDTEAEIDELEEAAKKAQIHESVDQFPKKYETRVGQKGVNLSGGQKQ
                     RLSIARALVRKPSILILDDSTSALDVKTESALWDALEGERATMLVVTQKIRTAKGADK
                     ILLLEDGEVSGYGTHDELLKQSALYRKIAESQSEEEAMADAKGNS"
     gene            775..>2193
                     /locus_tag="VFX34_01685"
     CDS             775..>2193
                     /locus_tag="VFX34_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563644.1"
                     /translation="MLKAIRKPFGYEPILKKEDLTKSAKKKDDRASDWKSVLLRIWKL
                     VDEQRGLLILVLSLVLVSSVLALLGPFLIGKIIDIHITEMQFEGLGTKILWLIGIYFG
                     LSAALYLQSFWMVGIAQQTIFRLRTNLFAHLQKLPVTFFDRRQHGELMSRVTNDIENV
                     SQTLNSSFIQVFSSILTLTGTLIVMLVLSPILTLLTMIIVPVMFISIRWITRRTGRLY
                     KLQQAAVGELNGMIEETISGQRIVKAFSQEDRMMEEFREKSDRLRKTGFWALTYSGFI
                     PKVMNMLNNGSFAVVAGVGGILALNGHVTIGTIVIFTEYARQFTRPLNDLANQFNTVL
                     SAIAGAERVFRIMDEPIEKDEATGHEDTKLRGDVEFRKVSFGYAVETDGYTIEDVSFH
                     VRPGETAALVGATGAGKTTIMQLLARFYEADKGEILIDGIPISELPRKTLRSQMAFVL
                     QDPFLFEASVKENIRYGKLDATD"
BASE COUNT          601 a          450 c          606 g          536 t
ORIGIN      
        1 atggtggaaa tggtacgagt cttcttggct tggagggcga acttcgtttt gagaatgttt
       61 ccttccgcta tccacatacg gacggggaag aggggattct taagaatgta tccttccatg
      121 tggcgccggg tgaaaagctc gcgattatgg gagcgacggg ctccgggaaa tcaaccctcc
      181 tgaacttgat tccaaggatg ttcgacgcga cggaaggcaa gatttatgta gggggcaaag
      241 acgttcagga atggccgctg aagaatctac gggaagcgat cgggtatgtc ccgcagcaat
      301 ccatcctctt tacaggttcg attcttgaga acttgtcatg gggagatacg gaagcggaga
      361 ttgatgaatt ggaggaagcg gcgaagaagg cgcaaatcca tgaatcggtc gaccagttcc
      421 cgaaaaagta tgagacccgt gtcgggcaaa aaggggtcaa cttgtccggt gggcagaagc
      481 agcgtctttc cattgcaagg gcgcttgtcc gtaagccatc cattctcatt ctggacgaca
      541 gtacaagtgc attggatgtg aaaacggaat ctgcactttg ggatgcgctg gaaggcgagc
      601 gtgcgacaat gcttgttgtg acgcaaaaaa tccggacagc gaaaggtgcg gataaaatcc
      661 ttctcctgga ggatggcgaa gtatccggat atggtacaca tgatgaatta ttgaagcaat
      721 ccgcattgta tcgaaaaata gcggaatcgc aatcggaaga ggaggcgatg gcggatgcta
      781 aaggcaattc gtaagccatt cggctatgaa ccgattttga aaaaagaaga tttgacgaag
      841 tcagcaaaga agaaggatga ccgggcgagc gactggaaat cggtcctgct acggatttgg
      901 aagcttgttg atgaacagcg cggactgttg attttagttc tatcacttgt cttggtcagt
      961 tccgtgctcg cacttcttgg tccgttctta atagggaaaa tcattgatat tcatatcaca
     1021 gaaatgcaat ttgaagggct cggaacgaag attctctggc tcatcggcat ttactttgga
     1081 ctttctgcag cgctctatct ccaaagcttc tggatggtag gaattgcaca gcagacaatt
     1141 ttccgtttgc gaacgaattt attcgcacat ctacagaagc tgccggtaac gttttttgac
     1201 cgcaggcaac atggagaact gatgagccgt gtaacaaatg atattgagaa cgtcagccag
     1261 acattaaata gttcgttcat tcaagtattc tccagtattt taacgctcac gggaacatta
     1321 atcgtcatgt tggtgctaag ccctatattg acgttactta ccatgatcat cgttcctgtt
     1381 atgtttattt ccattcggtg gattacccgc cggacaggaa ggctgtataa gttacagcaa
     1441 gcggccgtgg gtgaattgaa cggaatgatt gaagaaacaa tttccggaca gcgtattgtc
     1501 aaagcgttct cccaagagga tcgaatgatg gaggagttcc gtgaaaagag tgaccggctg
     1561 cgcaagacag gtttctgggc actcacctat tcaggtttta ttccaaaagt catgaacatg
     1621 ctgaataacg gaagctttgc agttgtagca ggggtcgggg gaattttagc ccttaatggt
     1681 cacgttacaa tcggaaccat tgtgattttc actgaatacg cccgacaatt tacaagaccg
     1741 ctcaacgatt tggcgaacca atttaacaca gtactctcgg cgattgcagg agcggagcgg
     1801 gtgtttcgaa ttatggatga gccaatcgaa aaagatgaag caacagggca cgaagataca
     1861 aaactgcgtg gagacgtcga gttccggaag gtgtcgtttg gctacgcagt agaaacggac
     1921 ggctatacaa ttgaggatgt cagcttccat gtgaggccgg gtgaaacggc ggctctcgtt
     1981 ggcgccacag gggcagggaa aacaaccatc atgcagctgc ttgcaaggtt ttatgaagcg
     2041 gacaagggtg agattctcat tgacggaata ccgattagcg agcttccgcg aaaaacgctt
     2101 cgcagccaaa tggccttcgt tctacaggat ccattcctgt ttgaagcatc tgtgaaggag
     2161 aatatccggt acggaaagct ggatgctacg gat
//
LOCUS       DASULI010000073         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1937701,
            whole genome shotgun sequence.
ACCESSION   DASULI010000073 DASULI010000000
VERSION     DASULI010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937701"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..885
                     /locus_tag="VFX34_01690"
     CDS             70..885
                     /locus_tag="VFX34_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEX5563645.1"
                     /translation="MEWKNIYRGFLMGISDLIPGVSGGTIAFILGIYDRLLLAISGFF
                     SREWKKHIGFLLPLGIGIGLTLLLFSRVIDFLLKDYPQPTQFFFLGLIIGVVPYISRQ
                     AGVRKNFKIGHFILLLIVGAALASTAFIKPADTGIITSLTAQNTLGLFLAGWAGSMAM
                     LLPGVSGSFVLLLLGVYATAINALSTLNIPIIIVIGAGVIVGFIVSSRIIHFLLERFT
                     HAMFAIIIGLIIGSVFVIYPGIPESGTPFVMSVIALITGLIVANIFNSASQSK"
     gene            complement(878..1216)
                     /locus_tag="VFX34_01695"
     CDS             complement(878..1216)
                     /locus_tag="VFX34_01695"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX5563646.1"
                     /translation="MESVKALPTPKDRLLNTCFLLFCLCMIDAVFTDFGIRYGHIEEA
                     NPFVRSLYDVNVIAFYMLKATLPILLFILMHYLKPSYLVRNLLIVALIIYTLVILIHI
                     SWMIIILAFI"
     gene            complement(1377..2873)
                     /locus_tag="VFX34_01700"
     CDS             complement(1377..2873)
                     /locus_tag="VFX34_01700"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX5563647.1"
                     /translation="MTNNDNHSQKKLTTAAGAPVVDNQNSMTAGPRGPVLLQDVWLIE
                     KLAHFDREVIPERRMHAKGSGAYGTFTVTNDITQYTRAKMFSEVGKKTDMFARFSTVA
                     GERGAADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPLQFPDLNHAVKRDPRTN
                     MRSATNNWDFWTSLPEALHQITIVMSDRGIPKTYRHMHGFGSHTYSMINANNERVWVK
                     FHFRSQQGIENLTDEQATELIGTDRETHQRDLLESIDNGNFPKWKMYIQVMTEEQALN
                     MPYNPFDLTKVWYKGDFPLIEVGEFELNRNPDNYFAEVEQAAFTPAAIVPGIGFSPDK
                     MLQARLFSYGDAQRYRLGVNHHQIPVNAPKCPFHSFHRDGAMRVDGNHGSRTSYEPNS
                     FGEWKEQPDFKEPPLKIYDDAAHWDFREDDDDYFTQPGKLFNLMTEEQKELLFGNTAR
                     NLGDAPNEIKYRHIRHCHQADPAYGEGVAAALGISMDDVLVKQEVITK"
     gene            complement(3061..3846)
                     /locus_tag="VFX34_01705"
     CDS             complement(3061..3846)
                     /locus_tag="VFX34_01705"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF002853.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX5563648.1"
                     /translation="MKKMIFKRFVELSGHPASSALLKTIARSRLSRKLIKPFAHAYRI
                     NEEEAEFPIDHYDSLQAYFTRNLKKGSRFIDIHSDTLISPVDGVVTEFGTIDTEQKFM
                     IKDHLYSIKQILGDEKRAIPFKGGFFYVFYLSPSHYHHFHYPYDGELLDRYALGTTSY
                     PVNDLGLRWGNQPFATNYRIISELSTTHGRMAMIKVGALNVNSIHILDSSTTCTKGEE
                     FGYFSFGSTVILFIEKNGDFHPTVPMNSEVKMGQSVGKWSSTS"
BASE COUNT         1063 a          873 c          932 g         1211 t
ORIGIN      
        1 ttatatggct cacggcaaac ggtaaatggt atactactat cattgaaaag atattggagg
       61 aactgcagta tggaatggaa gaacatttat cgtggattcc tgatgggaat cagcgacttg
      121 attccgggag tgagcggggg gacgattgcg ttcattctgg gcatatatga tcgattacta
      181 cttgcaatca gcgggttttt cagtcgtgaa tggaaaaagc atatcggttt tttactgccg
      241 cttggcatag ggatcggttt gacattgttg ctcttcagta gggttatcga cttcttgttg
      301 aaagactatc ctcaaccgac gcaatttttc tttttgggtt tgatcatcgg tgtcgtgcca
      361 tacatttcga ggcaggcggg tgtgaggaag aatttcaaaa tcggccattt tatcctgctg
      421 ctgatcgtcg gtgccgcgct ggcttcaacc gccttcatta aaccggcgga tacaggcatc
      481 attacttcgt taacggcgca aaatacgctt ggcttgttct tagcaggatg ggcgggaagc
      541 atggctatgc tgttgccggg tgtgagcgga tcatttgttt tacttttgtt gggcgtttac
      601 gctacggcaa tcaatgcact ttctactttg aacatcccga tcatcattgt gatcggggca
      661 ggggtcattg tcggattcat tgtaagcagc aggataatcc atttcctatt ggaaagattc
      721 acccatgcca tgttcgccat catcatcggc ttgatcatcg gttcggtttt cgtcatttac
      781 ccgggcatac ctgaaagcgg gacgccattt gtaatgagcg ttatcgccct catcaccggt
      841 ttgattgtcg cgaatatatt caattcggcg agtcagtcta aatgaatgca aggatgatga
      901 tcatccaaga tatgtggatg agaatgacta aagtataaat gattaaagca acgataagta
      961 agttccttac taaataggat ggtttcaagt aatgcataag gatgaatagc agtattggaa
     1021 gagttgcctt cagcatatag aatgcgatga cgttcacgtc ataaagtgaa cggacgaatg
     1081 gattcgcttc ttcaatatgg ccatagcgga tgccgaaatc agtgaagact gcatcaatca
     1141 tgcaaaggca aaatagaaga aagcacgtat tcaataaccg atccttgggt gttggcaacg
     1201 ctttgaccga ctccatatat atcactcctg acttaattgg catatatcag ctaggatatg
     1261 gagggggtag acgatttatt caaagggcgt cataaagatt ttccgcttct attataaata
     1321 tcgaattgag gcatgaaaaa tgccaacgaa aggagaaatc cttccgttgg cattttttat
     1381 ttggttataa cttcttgttt gactaatacg tcgtccattg agattccgag tgccgctgca
     1441 acaccttctc cataagcagg atctgcctgg tggcagtggc ggatatgtcg gtatttgatt
     1501 tcatttggag catcgccaag gttgcgtgca gtattgccga acaataattc cttctgctcc
     1561 tcggtcatca aattgaacag tttgcccggt tgcgtgaaat aatcatcatc gtcttcccgg
     1621 aaatcccagt gtgctgcatc gtcatagatt ttaagcggcg gctctttgaa atcaggctgt
     1681 tccttccatt caccgaagct attcggttca tatgaagtcc tgcttccatg gttgccatcc
     1741 acccgcattg cgccatccct atggaagcta tggaatgggc acttaggagc gttgaccgga
     1801 atttggtgat ggttgacgcc aaggcggtag cgctgggcat ctccgtaaga gaacaacctc
     1861 gcttgcaaca ttttatctgg cgagaaaccg atgcccggca caatcgctgc aggtgtgaaa
     1921 gccgcttgct ccacttcagc gaaatagtta tccggattgc ggttcaattc aaactctcca
     1981 acttcgataa gagggaagtc tcccttatac cagactttcg tcagatcgaa cgggttgtaa
     2041 ggcatgttca gtgcctgttc ctcagtcatt acttgtatat acatcttcca tttcgggaaa
     2101 ttgccgttgt cgatgctttc caataggtcc cgttgatgtg tttcacgatc tgttccaatc
     2161 aattcggtag cttgctcgtc cgtcagattc tcaatccctt gttgggaacg gaaatggaac
     2221 ttcacccaca cgcgttcgtt atttgcattg atcatgctat acgtatggct tccgaagcca
     2281 tgcatatgac ggtacgtctt cggaatcccc cgatcactca tgacaatagt gatttggtgc
     2341 agtgcttccg ggagggaagt ccagaaatcc cagttgtttg tcgcgcttct catattcgta
     2401 cgcgggtctc ttttgacggc atggttcaag tcagggaatt ggaggggatc cctgaaaaag
     2461 aatacagggg tgttgttccc gacaagatcc cagttacctt cttccgtata aaacttaagt
     2521 gcgaatccgc ggatatcacg ttcagcgtca gcagcgcccc gttcaccggc aaccgttgaa
     2581 aagcgggcga acatatccgt cttttttccg acttcggaga acatcttcgc tctcgtatat
     2641 tgtgtaatat cattcgtcac cgtgaaagtt ccatatgcgc cagatccttt cgcgtgcatg
     2701 cggcgttccg gaatgacttc acgatcgaaa tgcgctagtt tttcaatcaa ccatacgtcc
     2761 tgtagaagga ctggccctct gggacctgca gtcatcgagt tttggttgtc cactacagga
     2821 gcaccagcag cagtcgtcag tttcttttga gaatgattat cattgttggt cattgataat
     2881 gcctcctaaa gttaatagtc tactatcttc attttcaata tagcataacc atcctatgaa
     2941 atctacagtt aattataatg attattgata aataatagat attgtgtaat tcacattata
     3001 tttaactgaa gagggcgaag gatttgaaat tttgatgaat agactatttg tgaaggcgca
     3061 ttaagaggtg gagctccact ttccaacgga ttgccccatt ttcacttctg aattcatagg
     3121 aactgtcgga tgaaaatccc cgtttttttc tataaacaat atgacggtcg atccaaacga
     3181 gaaatagccg aattcttcac ctttcgtaca agtagtggag gaatccaaaa tatgaatgct
     3241 gttcacattc agagccccca ctttaatcat tgccatcctg ccatgagttg ttgaaagttc
     3301 ggaaatgatt cgatagttgg tcgcaaaagg ttgattgccc cagcgcaatc caagatcgtt
     3361 gactgggtag gaagtagtcc caagtgcata tcggtccagt aactcgccat catatggata
     3421 gtggaaatga tgatagtggc tcggcgatag ataaaaaaca taaaagaatc cacctttaaa
     3481 tggaattgct ctcttttcat ctcccaaaat ctgctttata ctatatagat ggtccttaat
     3541 cataaacttc tgttccgtat cgatcgtccc aaattccgta accactccgt caaccgggga
     3601 aatcagagta tcggaatgga tgtcgatgaa ccgagaaccc tttttcaaat tgcgcgtaaa
     3661 ataagcttga aggctatcat aatgatcgat tgggaattct gcttcctctt cgttaatacg
     3721 gtatgcatgc gcaaacggtt tgatgagttt ccggctgagg cgggatctgg ctatcgtttt
     3781 taataatgcg gatgaggcgg gatgcccgct cagttcgaca aaacgtttaa atatcatttt
     3841 tttcatttca aagtctccta tgttttagat tcgtagtcgg caagataaca atactggagg
     3901 tatcatcatg tttttatcta gatacttaga ttatacgaaa attaaaggcc aattggcgaa
     3961 tgcaattacc attacgaact taagcttcgg aatcatcgcg attattctta tcgctcggga
     4021 actcagtcat atgagtctcg tgttcatttt ccttgcagct cttttcgacc gattcgacg
//
LOCUS       DASULI010000074         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1884657,
            whole genome shotgun sequence.
ACCESSION   DASULI010000074 DASULI010000000
VERSION     DASULI010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884657"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..513
                     /locus_tag="VFX34_01710"
     CDS             277..513
                     /locus_tag="VFX34_01710"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5563649.1"
                     /translation="MHVQFYTKKGCHLCDEAESMLKLVQEDYPLTWSTIDIEKDDESH
                     EKYMLMIPVVEKDGEVLLYGSIGYIDLVDLFENG"
     gene            639..1673
                     /locus_tag="VFX34_01715"
     CDS             639..1673
                     /locus_tag="VFX34_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEX5563650.1"
                     /translation="MIPKIIEAQRKLVPEMMELLHQRYKVLKFIEVAGPIGRRPLGQM
                     ADISERECRNLMEALRTEGLIRIAKEGATITDEGATVLDSLAPTIDEWTGQGLIARKL
                     AKLLGIKSVQIVPGDCDENPVGKSLIGKEAAMEFTNRIGEGGIIAVTGGSSIAAIPPH
                     IHSLENAGRLHFIAARGGVGDDIGLQANVIAASFAEACGGTYSTFYYPESLSAEAHEV
                     FKREATAIQMMEQYAKTDCVLHGIGDALRMADLRNTPENECEHLREQGAKGEAFGYYF
                     DSSGNVVHRIRTVGIQTEQLKNVPLILAVAGGKSKAEAIMSYMASAPRQTILVTDEGA
                     AHAMLEKMEG"
     gene            1725..>2151
                     /locus_tag="VFX34_01720"
     CDS             1725..>2151
                     /locus_tag="VFX34_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX5563651.1"
                     /translation="MTIKMAINGFGRIGRVVMREARKHDEIEIVAVNDLTDAQMLAHL
                     LKYDSVHGIFDAEVSSEDDYLIVDGKKIKVYAEKDPSQLPWGELDVDVVIESTGVFRD
                     EKALMKHIEAGAKKVILSAPAKGDLKTLVMGVNEDSYDPE"
BASE COUNT          618 a          438 c          578 g          517 t
ORIGIN      
        1 aaatcctcca tcccaaattt cttatatgca tttacccaat caatgatctg tttctttgac
       61 tttatgcaat gtttttcagc caatctttta tagccaagcg tccctctgag gtattcattc
      121 actaccatcg ccttaaagtc atccgcatat ttaaccataa taaaacaccc caaaagttag
      181 attttgactc taacttttgg ggtgcacttc aataactttt tgcggaacga cgtttttcat
      241 ttatactaaa agaaaacgca actcgaggtg ttccacatgc atgttcaatt ctacacaaaa
      301 aaaggttgcc atttatgtga cgaggcggaa tccatgttga agcttgtgca ggaggattat
      361 ccgttgacgt ggtcgacgat cgacattgaa aaggacgatg aaagccatga aaaatatatg
      421 ctgatgattc ctgtcgtgga gaaagatggc gaagtgcttt tatacggctc catcggctat
      481 atcgacttgg tcgatttatt cgaaaatgga tgaagaagtt tgaatttatt ggcggtaagg
      541 tatatactac taataagaat tatatttttt tgcactaggt gggacaatta atatttaggt
      601 gggcagaaaa tgtcctatgc ggaaagaggg gcttgtccat gatcccgaaa attatagagg
      661 cacaacgaaa gcttgtgccg gaaatgatgg agttattaca tcagcggtac aaagttttga
      721 agttcatcga agtggcgggc ccgatcggaa gaagaccgct tggccagatg gcagatatat
      781 cggaaaggga atgccggaac ttgatggaag ctttgcggac ggaaggactg attcgcattg
      841 ctaaagaagg tgcgaccatt acagacgagg gggcaactgt cctcgactcg cttgcgccga
      901 caattgatga atggaccggg caaggattga ttgcaaggaa actggccaag ttattaggaa
      961 ttaaaagcgt gcagatcgtc ccgggggatt gtgatgaaaa tcccgtaggt aaaagcctga
     1021 tcggcaagga agctgccatg gaattcacga atcggattgg tgagggcggc atcattgctg
     1081 tcacaggcgg aagctccatt gcggccattc cgccgcatat ccattcactc gaaaatgccg
     1141 gccgcttgca cttcattgcc gcaaggggag gtgttggtga cgacatcggc ctgcaggcga
     1201 atgtgatcgc tgcctccttc gcggaagcgt gcgggggaac gtatagcacg ttctactacc
     1261 ccgaatcgct cagcgcagaa gcgcatgaag ttttcaagcg ggaggctacc gcaatccaaa
     1321 tgatggagca gtatgcaaag acggattgtg tcctgcatgg aatcggagat gcactccgga
     1381 tggcggactt gcgcaacaca ccggagaatg aatgcgaaca tcttcgggag caaggagcga
     1441 agggcgaagc attcggctac tactttgaca gcagcggcaa tgtcgtccat cggatccgga
     1501 cggtcggcat tcagacggag cagttgaaga acgtgccgct tatcctcgct gtcgccggcg
     1561 ggaaaagcaa ggcggaagcg atcatgtcct acatggcaag cgccccccgc caaacgatcc
     1621 ttgtcaccga cgagggagcg gctcatgcca tgctcgagaa aatggaaggt taacttgctt
     1681 gcgaatgtaa atacttgaat tgaaacatag gaggtaatgg agaaatgacg attaaaatgg
     1741 caatcaatgg attcggacgt attggaagag ttgtaatgag ggaagcaaga aagcatgatg
     1801 agatcgaaat tgtggcagtc aacgatttga cggatgcaca aatgcttgcg catctgttga
     1861 aatacgattc cgtgcatggg atttttgacg cggaagtgag ttcagaggat gattatctca
     1921 tcgtcgatgg gaagaaaatt aaagtgtacg ctgaaaaaga tccatcccaa ttgccttggg
     1981 gcgaattgga tgtcgatgtc gtcattgaat cgacaggcgt attccgggat gaaaaagcac
     2041 tcatgaagca tatcgaagcg ggagcgaaaa aagtaatctt gtctgctcct gcaaaagggg
     2101 atctgaaaac cctcgttatg ggtgtgaatg aagactcgta cgatccggaa a
//
LOCUS       DASULI010000075         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1756871,
            whole genome shotgun sequence.
ACCESSION   DASULI010000075 DASULI010000000
VERSION     DASULI010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756871"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1079)
                     /gene="trpS"
                     /locus_tag="VFX34_01725"
     CDS             complement(93..1079)
                     /gene="trpS"
                     /locus_tag="VFX34_01725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5563652.1"
                     /translation="MKTIFSGVQPTGTVTLGNYIGAFKQFTELQHEYDCRFCIVDQHA
                     ITVQQDPAELAKTIRSLAAIYIASGIDPEKSLLFIQSEVPAHAQAGWMMQCISYIGEL
                     ERMTQFKDKSDGKEGVSAGLLTYPPLMAADILIYNTDIVPVGDDQKQHVELTRDLAER
                     FNRRYGEVLTIPEVRIPKHGARIKSLQDPLKKMSKSDPNKKGTISLLDTPKQIEKKIK
                     SAVTDSDGIVKFDVENKPGVSNLLTIESALSGIAIADLESKYEGLGYGAFKTGVAEAV
                     VGHLTPIQERYEELLNSTELDVHLDRGAEKANAIASATLKKMESAMGLGRRR"
     misc_binding    complement(1124..1360)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1556..2761
                     /locus_tag="VFX34_01730"
     CDS             1556..2761
                     /locus_tag="VFX34_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX5563653.1"
                     /translation="MKKMKWISSLMLASAVAMPLTVQADEAGAEIEFAERTYGFETID
                     EGIVASSTLYSYDSGLVFEYPDAVRGIYVTGHSAGGARFERLLKLIASTDLNAMVIDI
                     KDDFGNLTYKPKEDSPLFNMDIGKPYIKDPHKMLETLEEQKVYPIARIVVFKDSVLAE
                     KHPELSFMDGDKVWKNGRKESFVNPFMKEVWDHNVEIAIEAAKMGFKEIQFDYVRFPE
                     GFEKRHETLKYSMGDYKESDLDPVQRRVEAVTDFVAYAREQLKPYGVDVSVDIFGYSA
                     TLPEAPGIGQNFSKISENVDVISSMIYPSHWTSYFGIAKPDLEPYRLVTEYAKVENAK
                     LGALESPPVSRPWLQDFSAPWLGKGNYLRYGKEEVEAQIKALKENGINEYLLWNANNN
                     YTEGVDYTP"
BASE COUNT          804 a          679 c          736 g          798 t
ORIGIN      
        1 atcgttaggc tatagctgga agcagattct gctgttcatc atccaaacgg ccgatttcac
       61 tcttttcgtt atggcagtca tacttctcgt tattaccgtc ttcttccgag tcccatcgcg
      121 gactccattt ttttaagtgt agcggaagcg attgcattcg ctttttcagc gccgcggtca
      181 agatgcacgt caagctccgt ggaattcaga agctcctcgt agcgttcttg gattggtgtg
      241 agatgtccga caacagcctc ggcaacgcct gttttgaatg cgccatagcc aagcccttcg
      301 tatttcgact cgaggtcggc aattgcgatg ccgcttaacg ctgattcgat tgtcagaagg
      361 ttggacaccc ctggcttgtt ttcgacatcg aatttcacga tgccgtcaga atccgtcact
      421 gcgctcttga ttttcttttc gatttgcttc ggtgtatcca atagcgaaat cgtccctttt
      481 ttattcggat ccgatttgct catcttcttc aacgggtctt gcaatgattt gatgcgcgcg
      541 ccgtgtttcg gtatgcgcac ttccgggatg gtgagcactt cgccgtatcg tctattgaaa
      601 cgttcggcca agtcccgcgt cagttcgacg tgctgcttct gatcgtctcc gaccggcacg
      661 atatccgtgt tgtagatgag aatatccgct gccatcaatg gcgggtacgt gagcagccct
      721 gcagagacgc cttctttccc atcggattta tccttgaatt gggtcatccg ctcaagttca
      781 ccgatatacg aaatgcattg catcatccat cctgcttggg cgtgcgcagg cacttccgat
      841 tggatgaaca gcagtgattt ctcaggatcg atgccgctgg cgatatagat ggcagcgagt
      901 gagcggattg tcttcgccaa ttcagccgga tcttgctgta cagtgatggc atgctggtcg
      961 acgatgcaga accggcagtc gtactcatgc tggagctctg tgaattgctt gaatgccccg
     1021 atgtagttcc ctaatgttac tgttcctgtt ggctgaacgc ctgaaaaaat agttttcatc
     1081 caaattcctc ctctgaaaat aaaaaaacac cagccatccc taaaaaaggg acgactgatg
     1141 tgcatcaacc gcggtaccac ccgaatcagc ctgcaagcaa gcaggccact caaacccctt
     1201 aacgcgggga gacgtgaaag gctactggac gatggatgaa catctttttc gccaatcaag
     1261 ctcaggaacc caattcctct tgtccgtcat gcctgttcgc accaaccaca ggctctcttg
     1321 aatgccgcga caagaatact ctttccgtct tcgcttttcg taatagttgt caaaagtata
     1381 atatggcatc ggaaataaag caactgttaa atagtttcca cacatgatta gttgacggcg
     1441 cacgcatttt aaggagtatc caacaaaaaa attggttttt cgacaaaaaa tatggggaat
     1501 agatgttcat tgcattttcg ctttgataga atgattgaaa gagaggaagt gtcatatgaa
     1561 gaaaatgaaa tggatcagct ctttgatgct cgcaagtgca gtcgccatgc cgcttaccgt
     1621 gcaagctgac gaggcagggg ccgaaattga atttgctgaa cgtacatacg gatttgaaac
     1681 gatagacgaa ggaatcgttg ccagctcaac cttatacagt tatgattccg gcctagtatt
     1741 tgaatatcca gacgcagtgc gtggaattta cgtgactgga cattccgcag ggggggcccg
     1801 gttcgagaga ttattgaaac tgatcgcttc cacggatttg aatgcgatgg taatcgatat
     1861 aaaagacgac tttggaaatt tgacgtataa gccgaaggaa gactcgccat tgttcaacat
     1921 ggatattggc aagccataca taaaagatcc gcacaaaatg ctggagacgc ttgaggaaca
     1981 gaaagtctat ccgattgcaa gaatagttgt attcaaagat agcgttcttg cggaaaaaca
     2041 tccggaactg tcattcatgg atggcgataa agtgtggaaa aacgggagga aagaatcgtt
     2101 cgtcaacccg ttcatgaaag aagtgtggga tcataatgtc gaaatcgcga ttgaagcggc
     2161 gaagatggga ttcaaggaaa tccagttcga ttatgtccgt ttcccggaag gtttcgaaaa
     2221 acggcacgaa acgttgaaat actcgatggg tgactataag gagtcggatc ttgacccggt
     2281 tcagcgcaga gtcgaagcgg tgacggattt cgtcgcgtat gcacgtgaac agctgaagcc
     2341 atacggcgta gacgtttccg tcgatatttt cggctactcg gcaactttgc ctgaagcgcc
     2401 tggaatcgga cagaacttct cgaagatatc cgagaatgtc gatgtcatct catcgatgat
     2461 ctatccgagc cattggacgt cctatttcgg catagcgaag cccgatctcg aaccgtatcg
     2521 actagtgacg gaatatgcta aggtggaaaa cgccaagctc ggagctctcg aaagtccgcc
     2581 ggtttcacgc ccttggctgc aggatttcag tgcgccttgg ctaggaaaag gaaattatct
     2641 ccgctatggg aaagaagaag tcgaagcaca aatcaaagct ttgaaagaaa atggcataaa
     2701 tgagtacctc ttatggaacg caaataataa ttatactgag ggtgtggatt acacgccttg
     2761 atcaacttcc ggaagcctga aagaatgggt ttccggatat tttttcttgg tttactgaaa
     2821 ctttttaagc ctccgcgtcg tactactaag tgtggagtgg tatttttcat tcaacgaatc
     2881 ctaaatgaga aaacgatttt tttgaagcga tgatgtttaa acatttcgtc cgcagggtat
     2941 aacagtaaag aagtgtcaaa gtacataacc aaaggagctg taaaccctat aatattgcgg
     3001 aaatgtgaca aggcaac
//
LOCUS       DASULI010000076         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_355866, whole
            genome shotgun sequence.
ACCESSION   DASULI010000076 DASULI010000000
VERSION     DASULI010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355866"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1703)
                     /gene="guaB"
                     /locus_tag="VFX34_01735"
     CDS             complement(237..1703)
                     /gene="guaB"
                     /locus_tag="VFX34_01735"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5563654.1"
                     /translation="MWESKFTREGLTFDDVLLVPGASEVLPKDVNLSVRLTEKMTLNI
                     PIISAGMDTVTESKMAIAMARQGGLGIIHKNMSIEEQAEQVVTVKRSENGVITNPFFL
                     TPEHQVFDAEHLMGKYRISGVPIVNNMDEQKLVGILTNRDLRFIQDYSLIINDVMTKD
                     NLVTAPVGTTLEDAEKILQQYKIEKLPIVDENGILKGLITIKDIEKVIEFPNAAKDKQ
                     GRLLVGAAVGVTSDTMVRVQKLVAAEVDVIVIDTAHGHSKGVLDTVAQIREEYPDLDI
                     IAGNVATAEATAALYEAGADVVKVGIGPGSICTTRVVAGVGVPQITAVYECASEARKQ
                     GKTIIADGGIKYSGDIVKALAAGGHVVMLGSLLAGTTESPGDTEIFQGRRFKVYRGMG
                     SVGAMEHGSKDRYFQEDAKKLVPEGIEGRMPYKGPISDTIHQLVGGIRSGMGYCGTKD
                     LHDLREKAQFIRMTGAGLRESHPHQVHITKEAPNYSIS"
BASE COUNT          524 a          556 c          443 g          628 t
ORIGIN      
        1 ttctgcttgt gcgggtgctg tgccgaacgt catcattaat acaaatggaa tgatcagcaa
       61 agcaacccat tttctcattt cccgtttcac caatatacct ccgtcattgc aattttccac
      121 tttatatttt atcacatcat ggacacgatt aggggattcg attaaaaaag cacctttccg
      181 ctcatatcaa gacgtgcgga aaggtgctga agtttcattt ttacagtttc cactttttac
      241 gaaatcgagt agttcggcgc ttctttcgta atatgcactt gatgcggatg gctttcacgc
      301 aaaccggcgc cagtcatgcg gatgaactga gctttttcac gcaggtcatg aagatccttc
      361 gttccgcaat agcccatgcc tgaccggatt ccgccgacaa gttgatggat cgtatcagaa
      421 attggccctt tgtaaggcat acggccttcg attccttctg gcacaagttt ctttgcatcc
      481 tcttggaagt aacggtcttt tgaaccgtgt tccatcgcac cgacagatcc catgccacgg
      541 tacactttga agcgtctgcc ttggaagatt tccgtatccc cagggctttc tgtcgttccg
      601 gctagcaagc ttccaagcat gacgacatgt ccacccgcgg ccaatgcttt cacgatatcg
      661 cccgagtatt tgatgccgcc atccgcaatg attgtttttc cttgcttacg tgcttcggaa
      721 gcacattcat aaactgcagt gatttgaggg acaccgacac ccgcaacaac gcgagttgta
      781 cagatggatc cgggaccgat tccgactttg acaacatccg cacccgcttc atataatgcc
      841 gcggtcgctt ccgctgttgc cacgttcccg gcaatgatat caagatccgg atactcttcc
      901 cggatttgtg caaccgtatc aagcacacct ttggaatgcc catgtgctgt gtcgataacg
      961 atgacatcga cttccgcagc tacaagcttc tgcacccgta ccatcgtatc ggatgtaacg
     1021 ccgactgcag ctcctacaag gagacggcct tgcttatctt tcgcggcatt cgggaattca
     1081 atcacttttt cgatatcttt gattgtaatt aagcctttta agatgccatt ttcatcgaca
     1141 atcggaagct tttcgatttt gtactgctgt aaaatctttt ccgcgtcttc caacgtagtg
     1201 ccgacaggag ctgtcactaa attgtccttt gtcatcacgt cattgatgat caacgaatag
     1261 tcttggatga aacgcagatc acgattcgtc aaaatcccga caagtttttg ttcgtccata
     1321 ttgttgacga ttggaacgcc tgaaatccga tacttgccca ttaagtgctc agcatcgaat
     1381 acttggtgtt caggagtgag gaagaaagga ttggtaataa cgccattctc cgaccgtttt
     1441 accgttacaa cctgctccgc ctgctcttca atgctcatat tcttatggat gatgccaagg
     1501 ccgccttgac gtgccattgc aatcgccatt ttcgactcag tcaccgtatc catccctgca
     1561 ctgatgatcg ggatgttcaa cgtcatcttc tctgtcaaac gaactgaaag attcacatcc
     1621 ttcggcaata cttctgaagc gccaggtaca agtaacacat cgtcgaacgt caatccttcg
     1681 cgagtgaatt tagattccca catatcgttt cctcctctaa tatcgttatc tgcgtttgaa
     1741 tattattaaa aggttatcag cgcctatggg acgtgtcaag gaggcaggaa taagaaaaaa
     1801 gattcctttt attcgctgaa ttgacttttc gattgctccg catgaagtac atcggttttt
     1861 tcacttgata acccgtatat agaagcccta ttcatcatta cctattaccg tttttattaa
     1921 tcggcaatat gacaacttta tatgcgatgc aaaaatctct ttttggcgtg cggggtgact
     1981 ctttgggcgc tgtagagctt aacttcctcg attgagacat taaatccttc ggattttcaa
     2041 acacaaaaaa accatccccc acaggggatg gccttggccc ggcgacgttc tactctcaca
     2101 gggggaagcc ccctactacc atcgacgctg aagagcttaa cttccgtgtt c
//
LOCUS       DASULI010000077         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_796686, whole
            genome shotgun sequence.
ACCESSION   DASULI010000077 DASULI010000000
VERSION     DASULI010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796686"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFX34_01740"
     CDS             complement(<1..794)
                     /locus_tag="VFX34_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5563655.1"
                     /translation="MGKWKTLLFLLMAAMLVLAACGEDKTEANGDGYRLVEKGKLTYA
                     ASGLYKPFNFEENGELTGFDMEIGAEIAKRMGLEPNPVTNPFETILQGLVANKYDAII
                     GSMAYTKKRAEQAAFTQPYYYSGGMIWVAEDNNEIKSPEDLKGKKIGVIAQSTYEEPA
                     KELSDNLQYYSSDVVALKDLTVENRLDAVITSDIVGFEAKDNGFAIKEVGSPLWVEQP
                     SVAVKKDNEKLRDAIDEALQEMIDDGTYEEISQKWFGRNLLDIDLE"
     gene            1129..2487
                     /gene="mgtE"
                     /locus_tag="VFX34_01745"
     CDS             1129..2487
                     /gene="mgtE"
                     /locus_tag="VFX34_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5563656.1"
                     /translation="MTLNGEKEDVSRAIVHVLKEGQKDTFQKIISELTPYEISRHYRG
                     LPRKRRILFLEWLSLDQLVALLRYLTRNEQLRVLKKIGAVRSTELLGGLKSDDLAHLL
                     SDLPEKEINELIAQLREEEKKDIRKKMKYPKKSAGRAMISNYVWVHESYTVEKTINKL
                     KHFRDFADYLNYVYVINDEKELVGVASYRDLLLSEPDKEITDVMTTDIVKVHENAKES
                     EVARMIGRHDFLSVPVVNDDNILVGIITADDVLDIVVREANEDIEMLFASGKEIDFHT
                     RPFVAAYKRLPWLILLLFIGLVSGSIIAKFEATLEAVVALAFFMPMIAGMTGNTGTQS
                     LAVVVRGLVTEELTMKKTLGLVFRELLVGIMLGVVCGAVISVIAYIWQDSFTLGIVVG
                     SSLVATLIIGTLAGTIIPLILSKFKVDPAVASGPLITTINDILSLLIYFGIATMFISK
                     LM"
BASE COUNT          702 a          650 c          634 g          754 t
ORIGIN      
        1 ttctctaaat caatatccaa cagattccgt ccgaaccact tctgcgaaat ttcttcatac
       61 gttccgtcat cgatcatttc ttggagcgct tcgtcaatcg cgtcacggag cttttcatta
      121 tcctttttca ccgcaaccga aggctgttcg acccataacg ggctgccgac ctctttaatg
      181 gcaaagccgt tatcttttgc ctcaaagccg acgatatccg acgtaatgac ggcatccaat
      241 cgattttcga ccgtcaaatc cttcaatgca acgacatcac tgctgtagta ttgaagatta
      301 tccgacaatt cctttgccgg ctcttcatag gtcgattggg caatgacacc gatcttcttc
      361 cccttcaagt cttctgggga tttgatttcg ttattgtctt ccgcgaccca gatcatgcca
      421 cccgagtaat agtacggctg tgtgaaagca gcctgctcgg cacgcttttt cgtgtaggcc
      481 atcgacccga tgatggcatc gtatttattc gcgacaagtc cttgaagaat cgtttcgaat
      541 ggattcgtca ccggattcgg ctcgaggccc atcctcttcg cgatctccgc cccaatttcc
      601 atgtcaaacc ccgtcaactc accattctcc tcgaagttga acggcttgta gagcccgctc
      661 gccgcatatg tcaatttgcc tttttcaaca aggcgatacc catcaccatt cgcttcggtt
      721 ttgtcttcac cgcaagccgc tagcaccaac atggccgcca ttagcaaaaa taatagcgtc
      781 ttccattttc ccatttgtaa aactcccttt ttcccgacaa gatgccgtct ttttttgctt
      841 agtaactctc tttccctatc attttcttgc taaacatata atttcagaaa aagaaaggtg
      901 attgacgacg aggattgcct tgcggaatgg gagtctgatg cgttatcatc gattctcgga
      961 agtttacaac cgaatctatg gagtttacaa ccgattcggg taatttatca ccgattgact
     1021 gcaacttatg cgaaggagca atccccattc agtttattta cataagaaat agggaaatag
     1081 acaagttagc aaggttttca tttacataag agtgaggtga agggagtcat gacgttgaac
     1141 ggggaaaaag aagatgtttc acgtgccatt gtgcacgtct taaaggaagg gcagaaggat
     1201 acttttcaaa aaatcatcag cgaattaact ccctatgaaa tatcgagaca ttaccgcggc
     1261 ctgcctcgca aacggcgcat ccttttctta gaatggctct ctttggatca gctcgtggcg
     1321 ttgctcagat atttgacacg caacgaacag ctgcgtgtat tgaaaaagat cggcgcggtc
     1381 cgctccacgg aattattggg tgggctgaag agcgatgatc ttgcccacct gctgtccgat
     1441 ttgccggaga aggaaatcaa tgagctcata gcccaactgc gcgaggaaga gaaaaaagac
     1501 atccgcaaaa agatgaagta tccgaaaaaa tccgccggcc gggcaatgat cagcaattat
     1561 gtatgggtac atgaatccta tacggtggaa aagacaatca ataaattgaa gcacttcagg
     1621 gatttcgcgg attacttgaa ttacgtatat gtcatcaatg atgaaaagga attggtcggc
     1681 gtcgcttctt accgtgactt gcttttgagc gagccggaca aggaaataac cgatgtgatg
     1741 acgacggaca tcgttaaggt gcatgagaat gcgaaggaaa gtgaggtcgc cagaatgatc
     1801 gggcggcatg atttcctttc cgtacccgtc gtcaatgacg acaatatttt agtcgggatc
     1861 attacagccg acgatgtact tgatattgtc gtccgagagg cgaatgagga tatcgaaatg
     1921 ttatttgcat ccggcaaaga aatcgacttc catacgcggc cgttcgtcgc ggcatacaaa
     1981 aggcttccgt ggcttattct gctcttattt atcgggctag tttcaggcag catcatcgca
     2041 aaattcgagg cgacgcttga agcggtcgtc gcattggcgt ttttcatgcc gatgattgcc
     2101 gggatgaccg gaaatacggg aacccaatca ttggctgtcg tcgtccgggg tcttgtcacc
     2161 gaggagttga cgatgaaaaa gacgctcggc cttgtgttcc gtgaactgtt agtgggcatc
     2221 atgcttggcg ttgtctgcgg tgcggtcatt tccgtcatcg cctatatttg gcaagacagc
     2281 ttcacacttg ggattgtcgt cggctcgtcg ctcgtcgcaa cgctgatcat cggaacgttg
     2341 gcgggtacca ttatcccgtt gattttgagt aaattcaaag tcgatccggc ggttgcgtcc
     2401 ggtccactca ttacgaccat caacgatatt ttgtctttat tgatctattt cgggattgca
     2461 acgatgttca tttcgaaatt gatgtgagtg aacaaacctt ttatttgtcg aaaggcattt
     2521 ggcgcaatca ggattcttga gggacttacc aaatttccgc ggcatcggga acgcggtacg
     2581 agtcaatcag ctgaaattta gtagatttcg cgccgaatgc ctttgacaaa ctagaatttc
     2641 gctttgtttc aagtaggcgc aaagtcccaa aatgatcata actccctgaa atatgcgcat
     2701 aacgggctta aagttgatca taactcactc aaatgtgcgc
//
LOCUS       DASULI010000078         3635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1367713,
            whole genome shotgun sequence.
ACCESSION   DASULI010000078 DASULI010000000
VERSION     DASULI010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367713"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..1027
                     /locus_tag="VFX34_01750"
     CDS             431..1027
                     /locus_tag="VFX34_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563657.1"
                     /translation="MGKTIYIVLTDTGTLLSKAIGMYTRKDLNHASIAFDEQLTEIYS
                     FGRKQQYNPFIGGFVRENVAIGIFQEADCAVYRCEVSMEAYMRIRQKIVQFELNQRDY
                     KYNFIGLFGVALNKEIRRKRAFFCSQFVATVMQESEMHRLLTRPSLTMPHHLAYLPHM
                     ELVYEGDLKTYLTKARGLQLERPLRMGLLKSLAFRLLA"
     gene            1307..2503
                     /locus_tag="VFX34_01755"
     CDS             1307..2503
                     /locus_tag="VFX34_01755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX5563658.1"
                     /translation="MTKTIDVQVNAQSIAAFKRGYPLILKDAVMNPEVLSEEGTLIRL
                     VDRYHKFVAKGYYGNQNKGIGWVLTRDQNEEIDFEFFESKISGALSRREPFFEDSETT
                     AFRVFNGEGDGIGGLTIDYFDGYYMVSWYSEGIYSMKHHVYGVLDKLVDYKAVYEKKR
                     FDTKGQYIEQDDFVKGTPGDFPIIVKENGMNFAVDLNDGAMTGIFLDQRDVRKALRDK
                     YSEGKHVLNTFSYTGAFSVAAALGGARETTSVDLAKRSLPKTIEQFSVNGIDYEAQDI
                     KVMDVFDYFRYAKRHELKFDVVVLDPPSFARSKKYTFSTAKDYPALLKDAIDITEKNG
                     VIIASTNNASFSMKKFKTFIEKGFADAGSRYKILEESSLPKDFRTNRDFPEFNYLKVV
                     ILQKLK"
     gene            2609..3229
                     /locus_tag="VFX34_01760"
     CDS             2609..3229
                     /locus_tag="VFX34_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HEX5563659.1"
                     /translation="MVEAVLHGAILALGLILPLGAQNVFVFNQGALQPKLLRALPVVV
                     TAGICDTILIVAAVSGVSLLILTFHWLETIMFAVGILFLLYIGISLWRSAPASMETEA
                     ERFSPKKQILFAASVSLLNPHAIIDTIGVIGSSALSYSGEARFAFTLTTIIISWCWFF
                     GLAVAGRLLGKMDTSGKFIGMLNKVSAVVVWGVAIYMAIRLFGKFF"
BASE COUNT          947 a          743 c          947 g          998 t
ORIGIN      
        1 gggagtggtt cagtttggaa gggaaagggt ttatacgatt gaatatggcg actgagccaa
       61 ggttagtcga gcttgctgtg aaaaatatat tgtcgaatct tgggaagtga aagctgtttt
      121 caattttttt caagaaaagc aagaaattcg aagcgattca tagtataata ggacaaccat
      181 cttagatgag cgatcagtac atccatacca accgatgctc tcgctttcct gcgggattga
      241 aaaatcttgg gaaagtgaaa ctctgcgtat agttaacacg tataatacat atatagaaaa
      301 ctgcttgaac tgtccgtgcc ccgtatcttg tctacaactt aggacttaga aggtttccac
      361 ctgcagacgg tttaggttcc cggaaaaatt gtacatactt gtagagagag atgagttagg
      421 ggttggaatg atggggaaaa caatctatat cgtacttact gacacgggaa cattgctgtc
      481 aaaggcgatt ggcatgtata cgcgaaagga tctgaatcat gcctccattg cattcgacga
      541 gcagctgacc gaaatttata gcttcggtcg gaagcagcaa tataatccgt tcatcggtgg
      601 atttgtacgt gaaaatgttg cgataggcat ttttcaagag gccgattgcg cagtataccg
      661 ttgcgaagtt tcgatggaag cgtatatgcg gatccgtcag aaaatcgttc aattcgagtt
      721 gaatcaacga gattataaat ataactttat cggtttgttc ggcgtagcgc tgaataaaga
      781 gatccggcgg aaaagggctt ttttctgttc gcaattcgtc gcgaccgtca tgcaggagag
      841 tgagatgcac cgactgctga cgaggcctag cctgacaatg ccgcatcatc tggcatattt
      901 gccacatatg gaacttgtgt atgaaggcga tttaaagacg tacttaacga aagcaagagg
      961 gctgcaattg gaacgtccgc ttcgtatggg actgttgaaa agcttggcgt tccgtctgct
     1021 tgcctgaagt gaaagcttct ccgatgagag aagctttttt catgtctagc tactggcgct
     1081 gaaagaatgc cttaatttct gcaaaaaccg caggaatacg gcaaatcgaa cccttcgcag
     1141 ttcgattgga tcgggacata agtcaaagac gctctgtggc aaaaagcgcc acgccgcacc
     1201 ttcgccttat gcctgaggcc ggcaggatgc cagtcatgca gtcgcccttc gcttttcttc
     1261 tgcatccatg cgataatgaa aaaaatagtg aaaaaaggat gacacaatga ctaaaacaat
     1321 tgatgtacaa gtgaacgcac aaagcatagc ggccttcaaa aggggctacc cattgatttt
     1381 aaaagacgca gtgatgaatc cggaagtatt gagtgaggaa ggtacactca tccgcctggt
     1441 cgatagatac cataagttcg ttgcaaaagg ctattatggc aatcaaaaca aaggaatcgg
     1501 ctgggtattg acgagggatc aaaacgaaga aattgatttc gagttttttg aatcaaagat
     1561 ttctggagct ttatcgagac gcgagccttt cttcgaagac agtgagacga ctgcattccg
     1621 cgtttttaac ggtgaaggtg atggaattgg tggtttgacg atcgattatt ttgacggcta
     1681 ttacatggtg agctggtata gtgaagggat ttattccatg aagcatcatg tgtatggcgt
     1741 gttggacaag cttgtggact acaaagcagt atatgaaaag aaacgttttg acacgaaagg
     1801 ccaatacatc gaacaggatg atttcgtgaa ggggacccct ggcgatttcc cgatcatcgt
     1861 gaaggaaaac ggtatgaact tcgccgtcga tctgaatgat ggggctatga cagggatttt
     1921 cctggatcag cgcgacgtgc ggaaagcgct gcgcgacaaa tattcggaag gcaagcatgt
     1981 gctgaatacc ttttcataca cgggtgcgtt ttcggttgcc gcggctcttg gcggggcacg
     2041 cgagacgacg agcgtcgatt tggcgaagcg cagcttgccg aagacgatcg aacagttcag
     2101 tgtgaacggg attgactacg aagcgcaaga tatcaaagtg atggacgtgt tcgattattt
     2161 ccgttatgcg aaacgccatg aattgaaatt cgacgtcgtc gttttagatc cgccgagttt
     2221 tgcacgctcc aagaaataca cattcagtac ggcgaaggat tacccggccc tgctgaagga
     2281 tgcaattgac atcacagaga aaaacggcgt tatcattgca tcgacgaata acgccagctt
     2341 cagtatgaag aagtttaaga cgttcatcga gaagggcttt gcggatgcag gttcgcgcta
     2401 taagatcctt gaggaatctt cattgccgaa ggatttccgc acaaaccgcg acttcccgga
     2461 attcaattac ttgaaagtcg tcattttgca gaagttgaaa taatacttgg aagccatctc
     2521 cgttcgggga tggttttttg gttatggcat tgcaattagt atgagaaagc ctttcaatag
     2581 atagaaagag ataaaagggg gagagtgaat ggtcgaggca gttctgcatg gcgccatcct
     2641 ggcacttggc ttgattctac cgttaggcgc tcaaaatgtg tttgtgttca accagggggc
     2701 attacagccg aagttgttga gggcacttcc cgttgtcgtg accgcaggga tttgtgacac
     2761 gattttaatt gtggcggctg tatcgggcgt ctctttgctc atcctgacat tccattggtt
     2821 ggaaacgatc atgtttgctg tcggcatcct gtttttgctg tacatcggaa tttccctttg
     2881 gcggagcgcc cctgcatcaa tggaaaccga ggcggaacgg ttttctccaa aaaagcagat
     2941 cttgtttgcg gcatctgtct ctttactgaa tcctcatgcg atcatcgaca cgatcggcgt
     3001 catcggatcg agcgccttaa gttattcggg agaggcccgt tttgcattca cgttgacgac
     3061 aatcatcatt tcatggtgtt ggtttttcgg actcgctgtt gcagggaggc tgctcggcaa
     3121 gatggatacg agcgggaaat tcattggcat gttgaataaa gtctccgccg tcgtcgtctg
     3181 gggagttgct atttatatgg ccatccggtt attcggcaag tttttctgag gagcgagggc
     3241 ttacagctcc ctcagctcct cattcaattt tgcagcctgc tttttgactt tgttatcccc
     3301 atatgccttt tgtaaattcc tgacctgcac gatctccatt cacaatccct ccgtcactgt
     3361 tctttataca ctcactatat acagaaacta taaatccgta tgccggtttt cgatgaccgg
     3421 tcgtttttcc gtggtcagtt gcatcaaagc caccgtgagt tgcatcggac gagtgcgtcg
     3481 ttggcgttat gcgcgaattt gggtgtgttt tgctcgagtt tggctgggtt atgcgtgaat
     3541 ttggcagggt tatgctcgac ttcggatgag ttatgcgcgt ttttgctgca cttatgctca
     3601 tttggggact tttgacgcat aacacgtcag atgtt
//
LOCUS       DASULI010000079         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_914227, whole
            genome shotgun sequence.
ACCESSION   DASULI010000079 DASULI010000000
VERSION     DASULI010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914227"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFX34_01765"
     CDS             complement(<1..493)
                     /locus_tag="VFX34_01765"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5563660.1"
                     /translation="MKRVYLVGFMGSGKSAIGKRLATLLNLPFYDMDTEISNQTGMTI
                     PQIFETYGEEGFREMETEFLRNFHEDYCIIATGGGVAMREENRKIMRRTGLVFFLNST
                     FRDIWRRISTDINRPIVQRSSRAELERLFQKRKPLYIQSAHFKVETTNRTLNEITQYI
                     ASQI"
     gene            complement(659..1108)
                     /gene="comGF"
                     /locus_tag="VFX34_01770"
     CDS             complement(659..1108)
                     /gene="comGF"
                     /locus_tag="VFX34_01770"
                     /inference="COORDINATES: protein motif:HMM:NF041002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HEX5563661.1"
                     /translation="MHIVLKGRNEGGYTLLEALFQLLIMCIFVQFLLLFFYWKQPIER
                     QLLDYYATEWELFAIDLQALLVDVEDFNVLIGDRAISFTTERGKIEVGQSNYVIRKRT
                     NSAGHIPLFTNVSSVFFTHEGHELNVQVTMLDGRKRERRFAVGFRQE"
     gene            complement(1093..1395)
                     /locus_tag="VFX34_01775"
     CDS             complement(1093..1395)
                     /locus_tag="VFX34_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563662.1"
                     /translation="MSDEKGYSWPESILSLVIITVIFGTLLPFYSHMAERLEMKKLGM
                     HAAESAYHGAILYHAYGMMAGSNRIEKTMYNWSVESEGICVTYHYIERELVKCISF"
     gene            complement(1388..1816)
                     /gene="comGD"
                     /locus_tag="VFX34_01780"
     CDS             complement(1388..1816)
                     /gene="comGD"
                     /locus_tag="VFX34_01780"
                     /inference="COORDINATES: protein motif:HMM:NF040982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HEX5563663.1"
                     /translation="MKKTDESGLTFLEMLLVLSVVSVLTAVILPIGDRWITKQSEKEA
                     LYTFIATIHHAQAYAMANETYTAIRFRNSGKSYSLFAPSTVTYSTIDFPEGMHLFNGR
                     TIEFHPEGHILNPGTIVLRTTNGDKRLTLQLQHGRVIVYE"
     gene            complement(1803..2123)
                     /gene="comGC"
                     /locus_tag="VFX34_01785"
     CDS             complement(1803..2123)
                     /gene="comGC"
                     /locus_tag="VFX34_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HEX5563664.1"
                     /translation="MKIVKKEEGFTLIEMMIVLLIISVLILIAIPNVTKHSKSIDEKG
                     CAAFVKMAQGQVEAYKMEHHKSPTSIQQLIDTKYLPEEAKCPDGRVLAIKDGEVSATA
                     NEED"
BASE COUNT          576 a          541 c          422 g          642 t
ORIGIN      
        1 taatctgtga cgctatatac tgcgtaattt cattcaatgt cctatttgtc gtctccactt
       61 tgaaatgagc gctttgtata taaagcggct tccgtttttg aaacagtctt tccaattcag
      121 ctctggatga acgctgaaca atcggcctgt tgatatcggt ggaaatcctt cgccagatat
      181 cacggaatgt cgaattcaag aagaagacaa gtcctgtcct tctcatgatt ttcctgtttt
      241 cctctctcat cgcaacaccg ccgccagtcg caatgatgca atagtcttca tggaaattgc
      301 gcaggaactc tgtttccatc tcccggaagc cttcttcccc gtatgtttcg aaaatttgag
      361 gaatcgtcat gcctgtctga ttggagattt cagtatccat atcgtaaaat ggcaaattca
      421 gcaatgtcgc caaccgcttt ccgattgcgc tttttccact tcccatgaaa ccgaccaagt
      481 atactctttt catatctttt acccgccttg cttcatcaac tttgatgttt ccttctcgta
      541 atagatttcg agtgaatcga atgttctgta tagattacta tgccatgaaa cgatagtaaa
      601 aagaaagagg ctgacgagaa atagcaaaat tagtgaagcg gctaaaacgg ctccttgctc
      661 attcctggcg aaacccaact gcaaatctcc tttcccgctt ccttccgtca agcattgtca
      721 cttgcacgtt aagctcatgt ccttcatggg tgaaaaacac acttgaaacg ttcgtgaaga
      781 gagggatatg ccctgccgaa ttggtcctct tgcgaatgac ataattactt tggccgacct
      841 cgattttccc tctctcagtc gtaaacgaaa tggctcgatc acctatgaga acgttgaaat
      901 cttcaacatc gactaataac gcctgcagat cgatagcaaa caattcccat tcagtcgcat
      961 aataatctag taattgacgc tcaatcggct gtttccaata gaagaacagg aggagaaatt
     1021 gaacgaatat gcacatgatc agtaattgga ataatgcctc cagaagcgtg tatccccctt
     1081 cattgcggcc tttcagaacg atatgcattt cacgagttcc cgttctatgt aatgataggt
     1141 tacacaaatg ccttcacttt caaccgacca gttgtacata gtcttttcaa tccggttcga
     1201 cccagccatc atcccgtatg cgtgatagag aatggcgccg tgataggcgg attccgctgc
     1261 atgcatgcca agtttcttca tttctagccg ctccgccata tgcgaataga acggcaatag
     1321 cgttccgaat atgaccgtaa tgatgacaag ggataaaatc gattcaggcc aagaataccc
     1381 tttttcatca ctcatagacg attaccctcc cgtgttgcag ttgcaatgtc aaccgcttgt
     1441 ccccattagt tgtccttaag acgatcgttc ccggattgag tatatggcct tccggatgaa
     1501 attcgattgt gcggccatta aacaagtgca tgccttccgg aaaatcaatc gtggaatagg
     1561 tcactgtgct aggagcaaac aaactatagg attttccgga attccgaaac ctgatcgccg
     1621 tatacgtttc gttcgccatt gcataggctt gcgcatgatg aattgtcgcg atgaatgtat
     1681 atagcgcctc tttctcgctc tgtttcgtta tccagcgatc gccgataggc agaataactg
     1741 ccgtcagaac agagacgaca cttaaaacca ggagcatttc cagaaatgtt aagccgctct
     1801 catcagtctt cttcattcgc tgtagccgac acttcgccgt cttttattgc aagtactctt
     1861 ccatccggac attttgcttc ttctggcaaa tacttagtat ctataagctg ttgaattgat
     1921 gtcggacttt tatgatgctc catcttatac gcctcaactt gcccttgagc cattttcacg
     1981 aatgctgcac accccttttc atcaatcgac tttgaatgct ttgtcacatt cgggatggct
     2041 atcaaaatca gaacggagat gatgagcagc acgatcatca tttcgattaa agtgaacccc
     2101 tcttctttct tgactatttt caaccatttc ctcctcctta gaaattgtca aacgtactgt
     2161 atatcggcag cagcaaagcc a
//
LOCUS       DASULI010000080         4935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1012716,
            whole genome shotgun sequence.
ACCESSION   DASULI010000080 DASULI010000000
VERSION     DASULI010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012716"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFX34_01790"
     CDS             complement(<1..290)
                     /locus_tag="VFX34_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5563665.1"
                     /translation="MKDEERLRIWIEQGNVTISQLFFQHYKALDIKDVDAVLLLQMCA
                     FQADGSQFPTPSDLAARMHLTENQVSEILQRLMQRGLLEIRQGQDENGVLFE"
     gene            complement(325..1620)
                     /gene="asnS"
                     /locus_tag="VFX34_01795"
     CDS             complement(325..1620)
                     /gene="asnS"
                     /locus_tag="VFX34_01795"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX5563666.1"
                     /translation="MKTIMIHEMPDHVGETVRIGAWLANKRSSGKIAFLQLRDGSGFV
                     QGVVVKEVVGEDVFAKAKSLHQESSLYVTAEVTEDERSSFGVELQVKEIEIIHEAVDY
                     PITPKEHGTEFLMDNRHLWLRSRKQHAIMKIRNEIIRSTFEFFHMNGFVKIDPPILTG
                     SSPEGTTELFHTKYFDEDAYLSQSGQLYLEAAAMALGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFVQHEESLEIQEQYVSHIVQSVLQNCPLELERLGRDVSKLEKIQAPFP
                     RITYDDAIKFLHEKGFDDIEWGDDFGAPHETAIAENYDKPVFITNYPKDIKSFYMQPH
                     PDRDDVVLCADLIAPEGYGEIIGGSERIYDYELMKQRIKEHNLDESAYEWYLELSKYG
                     AVPHSGFGLGLERTVAWISGVEHVRETIPFPRLLNRLYP"
     gene            complement(1644..2837)
                     /locus_tag="VFX34_01800"
     CDS             complement(1644..2837)
                     /locus_tag="VFX34_01800"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5563667.1"
                     /translation="MTKKLAARVSTLSPSTTLAITAKAKEMKESGIDVIGLGAGEPDY
                     NTPENILEAAYQSMKEGKTKYTPSGGLPALKDAVIAKLKTDQQLDYTRQEIMIGIGAK
                     HVLYTLFQVLLDPSDEVIIPTPYWVSYPEQVKLAEGVPVFVEGTSESRFKVTAEQIRN
                     AITPKTKAVIINSPGNPTGMIYSQDELQQIADVCKEKDIWIISDEIYEKLIYGSEKHI
                     SIAQLSDDAKKRTFIINGVSKSHSMTGWRIGYIAGDAEVVGAMTNLASHSTSNPTTTS
                     QYAAIEAYNGPQDAVESMRKDFESRLERIYPQLAAVPGFTVVKPQGAFYLLPEVTEAM
                     NKTGFSNVDDFASALLTEAKVAVIPGSGFGSPETIRLSYATSIDLLEEAVKRIRIFVE
                     ANWKE"
     gene            complement(2887..3366)
                     /locus_tag="VFX34_01805"
     CDS             complement(2887..3366)
                     /locus_tag="VFX34_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEX5563668.1"
                     /translation="MMNWIKFIAAFLLALSLIIIVIVFVNANAPFSDAHQKAEADALK
                     SGALKSVERTDIYNGTVSMVTVFGKDDEGNEKAVFIEGKTGEILDEVVMKNGIEAKAA
                     IETVKAELPVKKILHVVLGLEEGHPVWEVAFKGENGKLNYVYVFFENGEWWKRILNL"
     gene            complement(3433..>4935)
                     /locus_tag="VFX34_01810"
     CDS             complement(3433..>4935)
                     /locus_tag="VFX34_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX5563669.1"
                     /translation="HSNLIITNHSMLLSDSNREQRIFKNFSGLIIDEAHQFIHTAANS
                     NETVLSYMNWKYVMGQLSSDADGQLLHQMIKLHRKYGNYENRVFGRLAASFEQFTNVF
                     DDVAALLSNHRTQVKGGQQGNRMIFGLEELTGNESLFSTMAEKMNGYIEQVQIISSRL
                     EACRGKMSMKEQAFLSEWNYWLRELKIKAGEWVEIFLDEKTENFIVWIEKDKRSLPGS
                     LNIVKSPVEGSAITKEFIKNLKEEKIGIVWTSATMTINQDERFVSRQLGIACSVPLLT
                     FDAPAHFYDNAGIFIVEDMPSIQHVSQSDYIEAVADAVVQTVIATGGRLFVLFTSQDM
                     LRKTYELILESELLNDYALIAQGVSSGSRMKLLKSFRQFDKSILFGTNSFWEGVDVPG
                     EALSAVIIVRLPFSSPDEVVFKARAARLAASGSNPFTDLSLPEAILRFRQGFGRLIRS
                     SGERGFFIILDRRIDTKSYGKRFLNALPSVPVRKVSLEHMVNELEDCYNK"
BASE COUNT         1291 a         1214 c          986 g         1444 t
ORIGIN      
        1 tgttcgaaga gtactccgtt ttcatcctgg ccttgccgga tttccaacaa tcctcgttgc
       61 attaagcgct ggaggatttc ggatacttgg ttctccgtaa gatgcattcg cgcagcaaga
      121 tcggatggtg taggaaattg cgaaccatcc gcctgaaatg cacacatctg caatagaagc
      181 accgcgtcga catcttttat atccaatgct ttatagtgct gaaaaaagag ctgggaaatg
      241 gtgacatttc cctgctcaat ccaaatacgg agccgttctt catctttcat gccgaacacc
      301 tcactattaa taaatctgca tcaattaagg ataaagacga ttcaataggc gtgggaaagg
      361 aattgtttcc cttacgtgtt ccacaccgga aatccacgca accgtccgtt ccagtcccag
      421 cccgaagccg gaatgcggaa ctgcaccgta tttgcttaat tccaaatacc attcgtaagc
      481 ggattcgtcg agattatgct cttttatccg ttgcttcatc aattcgtaat cgtaaatacg
      541 ttctgaaccg ccgatgattt ctccataccc ttccggcgca atcaaatcag cgcataggac
      601 gacatcgtca cgatccgggt gcggctgcat atagaatgat ttaatatctt ttggataatt
      661 tgtaatgaag accggcttat cataattttc ggcaatcgca gtttcatgcg gcgcgccgaa
      721 atcatcgccc cattcgatat catcgaatcc tttttcatga aggaacttga ttgcatcatc
      781 ataagtgatc cgtgggaatg gcgcttggat tttttcaagt ttcgaaacat ccctgccaag
      841 ccgctcgagt tccaatgggc agttttgcaa tacagactgt acaatatgcg aaacatattg
      901 ctcttggatt tccaagcttt cctcatgttg cacgaatgcc atttcaggtt caatcatcca
      961 gaattcaatc aaatggcggc gtgttttcga tttctctgca cggaatgtcg gtccgaatga
     1021 gaatactttt cctagagcca tcgctgcagc ctcaagatag agctgtcctg attgtgacaa
     1081 ataagcatct tcatcgaaat acttcgtatg gaataattcc gtagttcctt ccggtgaaga
     1141 gcccgtcaaa ataggcggat cgatcttaac aaaaccgttc atatggaaaa actcaaatgt
     1201 tgagcgaatg atttcgttcc gaattttcat gatggcatgt tgcttacgag aacgcagcca
     1261 taaatgacgg ttatccatca aaaattcggt tccgtgctct ttcggggtaa ttgggtaatc
     1321 gactgcttca tgaatgattt cgatttcttt cacttgcaat tctacaccga atgaagaccg
     1381 ctcatcttcc gtcacttccg ctgtgacata tagggaagat tcttggtgga gagattttgc
     1441 tttagcgaaa acatcctccc caacaacttc cttaacgaca acgccttgga caaatcctga
     1501 accgtcccga agttgcaaga aagcgatttt accgctcgat cttttattcg ctagccacgc
     1561 tccgatccgg acagtttctc caacatgatc gggcatttcg tgaatcataa ttgttttcat
     1621 aagtacctcc gtgagtatga ctattattct ttccagttcg cttcaacaaa tatccggatg
     1681 cgcttaactg cttcctcaag cagatcgatc gaagtcgcat aggataagcg gatcgtttca
     1741 ggtgaaccga aaccggatcc cggtatgact gccacttttg cttcggttaa aagggccgac
     1801 gcaaagtcat ccacatttga aaagccggtt ttgttcattg cttcagtaac ttcaggcaac
     1861 aagtagaagg cgccttgagg tttgacgact gtgaatcctg gaaccgccgc cagctgagga
     1921 taaatacgtt caagcctcga ctcaaaatcc ttccgcatgc tttcaaccgc atcctgaggg
     1981 ccgttatacg cttcaatcgc agcatattgt gaagttgtag tcggattgga tgtcgaatgg
     2041 ctggccaagt tcgtcattgc accgacaact tctgcatctc ccgcgatata accgattctc
     2101 catccagtca ttgaatggga tttggaaacc ccgttgataa tgaatgtccg ttttttcgca
     2161 tcatccgaaa gctgtgcaat cgagatatgc ttttcacttc cgtaaatcag cttttcatag
     2221 atttcatccg agatgatcca aatatccttt tctttacaaa cgtccgctat ttgctggagt
     2281 tcatcttgcg aatagatcat tcctgtcgga ttacccggtg agttgataat gacagcctta
     2341 gttttaggag tgattgcatt tcggatttgc tcagcggtca ctttgaatcg ggattcagat
     2401 gtgccttcga cgaatacagg aacaccttcc gcaagcttca cctgctcggg atagcttacc
     2461 caatacggcg tcgggatgat gacttcgtcg ctcggatcca ataggacctg gaacaatgtg
     2521 taaagaacat gcttcgcccc gattccgatc atgatttctt gcctagtata atccagctgc
     2581 tgatctgtct tcagtttggc tatgaccgcg tccttcaatg cgggaaggcc accagaaggc
     2641 gtatattttg tcttgccttc tttcatggat tgataagccg cttccaaaat gttttctgga
     2701 gtattgtaat cgggttctcc cgctcctaaa ccgatgacgt cgatgcccga ctccttcatc
     2761 tctttcgctt ttgccgtaat ggcaagagtc gttgatggtg ataacgtact cactcttgct
     2821 gctaatttct ttgtcaattg aatcgctccc ttttgtccct tcatctattg aatgtagcgg
     2881 atttccttaa agatttaaaa tccgcttcca ccattcgccg ttctcaaaaa agacatatac
     2941 ataattcaat ttgccgtttt cacctttgaa cgcgacttcc caaacaggat ggccttcctc
     3001 caagccaagg actacatgga ggatcttctt cactggcagc tcggccttga ccgtttcaat
     3061 tgcggctttg gcttcgatgc catttttcat aaccacttca tcaaggattt ctcctgtttt
     3121 cccttcgatg aaaaccgctt tctcattacc ttcgtcatcc ttgccaaata cagtcaccat
     3181 cgaaactgta ccattataaa tatcggtccg ttccacggat ttcaaagcac ccgacttcaa
     3241 ggcatccgct tccgcctttt gatgggcgtc tgaaaaaggg gcattggcat ttacaaaaac
     3301 gatgacaata atgataagag ataacgccaa aaggaaagct gcgatgaatt tgatccaatt
     3361 catcataagc atcacttcct atatacaaac tgcaaattgg aaagctttac gcaatgtgct
     3421 ttctattgat tcctatttat tataacaatc ttcgagctca ttcaccatat gttctagtga
     3481 cactttccgg acaggaacag atggcaatgc gttcaaaaat cgcttcccat aagatttcgt
     3541 gtcaatcctt ctatccaaaa tgataaaaaa gcctctctct ccagatgacc ggatcagtct
     3601 tccgaatcct tgccggaacc ttagaatagc ttccggcaag gacaaatctg taaatggatt
     3661 ggatccgctt gctgccagtc ttgcggccct cgccttgaaa acgacttcat caggcgatga
     3721 aaaaggcagc cggacaataa ttactgcaga taacgcctcg ccgggaacgt cgacaccttc
     3781 ccaaaaactg ttcgttccga acaagatcga tttgtcaaac tgacggaatg attttaaaag
     3841 tttcatgcgg ctgcctgaac tgactccttg cgcaatgaga gcataatcat tcaataactc
     3901 actctccaaa atcaattcat acgtttttcg caacatatcc tgggaggtga aaagaacgaa
     3961 caaccttccg cctgtcgcaa tcactgtttg aacaacggca tccgcgacag cttcgatata
     4021 gtcactttgg gatacatgct ggatagaggg catatcttca acgatgaata ttcccgcgtt
     4081 gtcatagaaa tgcgccggag catcgaatgt cagtaaaggc acactgcacg ctatccctaa
     4141 ctgcctggaa acaaaacgct catcctgatt gattgtcatt gttgccgacg tccagacaat
     4201 tccaatcttt tcttctttaa ggttcttgat gaattccttt gtaattgctg aaccttcgac
     4261 agggcttttg acgatattca aacttcccgg caagctccgt ttatccttct caatccagac
     4321 gatgaaattc tccgtcttct catcaaggaa aatttcgacc cattctccag ccttaatttt
     4381 taattccctt aaccaataat tccattctga taaaaatgcc tgttctttca ttgacatttt
     4441 tcccctgcac gcttccaacc gggaactgat gatttgcact tgctctatat agccgttcat
     4501 cttttccgcc attgttgaaa atagcgattc attcccggtt aattcctcaa gaccgaaaat
     4561 catccggttg ccttgctgtc ctccttttac ttgagtccgg tgattcgaca acaaggcggc
     4621 gacatcatca aatacatttg tgaactgttc aaaggaggct gcgagccttc cgaacaccct
     4681 attttcataa ttcccatatt ttctatgaag cttgatcatt tgatgcaaaa gttggccatc
     4741 cgcgtccgag cttagctgac ccattacata cttccagttc atgtaggaga gaaccgtctc
     4801 attggaattg gcggccgtgt ggatgaattg atgagcttca tcaatgatca aaccggaaaa
     4861 gtttttaaaa atcctttgtt cgcggttgga atcggacaat agcatcgagt ggttcgttat
     4921 gatgagattg gaatg
//
LOCUS       DASULI010000081         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1145541,
            whole genome shotgun sequence.
ACCESSION   DASULI010000081 DASULI010000000
VERSION     DASULI010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145541"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VFX34_01815"
     CDS             <1..102
                     /locus_tag="VFX34_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5563670.1"
                     /translation="IGSRGLNIFQEMVLGSVSHKVAKRAKIPVLIVK"
     gene            320..682
                     /locus_tag="VFX34_01820"
     CDS             320..682
                     /locus_tag="VFX34_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5563671.1"
                     /translation="MKKDTCEVTMVHEAEVNRVRENLPDMTNMVALFKALGDETRLKI
                     AFALTIEEELCVCDVAAVIGSSTATASHHLRYLRDHSLAKSRRKGKLVYYALADDHVR
                     QLVTIAHEHAEEGDCDGV"
     gene            672..>2679
                     /locus_tag="VFX34_01825"
     CDS             672..>2679
                     /locus_tag="VFX34_01825"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5563672.1"
                     /translation="MAFEKQAEYRLQNLSCANCAIKFEKNIRELPEVSDVTVNFGASK
                     LLVKGSATVEELEAAGAFDGIKVIPAAEKRVEEQTPFFKRRENIVTLLSLVFLLIGTY
                     VSFRYAEHHPAAIALFAVSIAIGGASMFKSGLQNLVRLDFDMKTLMTIAVIGAAIIGE
                     WREGAVVVFLFAVSEALEAYSMNKARQSIKQLMDIAPSTATVLRDGNQVELDTEDIVI
                     GDTLLVKPGQKIAMDGIVRTGMSSINEAPITGESMPAPKTVGDEVFAGTVNEEGALEI
                     EVTKLVGDTTIAKIIHLVEEAQAEKAPTQKFVDRFAKYYTPIIIGIAFLVAIVPPLFT
                     GDWHTWVYQGLAVLVVGCPCALVVSTPVAIVTAIGNAARHGVLIKGGVHLEQAGRIKA
                     IAFDKTGTLTKGYPDVTNVVSLNGMDEKELISIAAAVESMSQHPLAKAILSYSKRIGA
                     PIKEATNFQSVTGKGAYATVEGVTVSIGSIDWMEELGDVPGTLRESSTSLQAEGKTVV
                     AVALDGVLAGIIGISDPIRSTSASVLEKLKNIGVRQTIMLTGDNPKTAQAIAEKLGVT
                     DVRAKLMPQDKLSAILHMRQQYGTVAMVGDGINDAPALAAADVGIAMGGAGTDAALET
                     ADIALMADDLEKLPYTIKLSRKALRIIKENIMFALGLKIVALLLI"
BASE COUNT          743 a          544 c          732 g          660 t
ORIGIN      
        1 atcggtagtc gcgggttgaa catctttcaa gagatggtac tcggaagtgt aagccataaa
       61 gtggcaaagc gtgctaagat tcctgttttg atagttaaat aaattcccga caccacaaac
      121 gaatgcttcg ttttgggtgt ctttttttct tatgccaacc aagaaagtgc agtagtagga
      181 caacccattt gtaacagtgt tcaagtgtac gtattcggaa tgattcctga atacgcaaga
      241 cagtttacaa tcgaacgaga gtggacatat actgataata caatcaaatg gacgtttgaa
      301 tgaaggtggg tgggaaatca tgaaaaaaga tacttgtgaa gtgaccatgg tgcatgaagc
      361 ggaagtgaat cgggtccggg aaaacttgcc cgatatgaca aatatggttg ccttgtttaa
      421 agcgcttggg gatgaaacga gattaaaaat tgcgttcgcg cttacaattg aggaagagct
      481 atgtgtatgt gatgtggcag cagtgatcgg atcgtcaact gcgacggcat cccatcatct
      541 tcgctatttg cgagatcact ccctcgcaaa atcccgtcga aagggaaagc ttgtgtatta
      601 tgcgctagca gatgaccacg tacgtcagct tgtaacgatt gctcacgaac atgcggaaga
      661 aggggattgc gatggcgttt gaaaagcagg cggagtatcg ccttcaaaac ttatcatgcg
      721 cgaactgcgc gattaaattt gaaaagaata tacgggagct gccggaagta tcagacgtga
      781 ccgttaactt tggtgcttcg aagcttttgg taaaaggctc agcaactgtt gaagagctag
      841 aggctgccgg agccttcgat ggaattaagg tcattccggc agcggagaag agggtggagg
      901 agcaaacccc gttctttaaa cgaagagaaa atattgttac gttgctttcg ctcgttttct
      961 tgctcatcgg tacgtatgtt tcattccgat atgctgaaca tcatccagct gcaattgcac
     1021 tgtttgctgt ttcaattgcg attggtggag catcgatgtt caagagtgga cttcaaaact
     1081 tagtccgact tgatttcgat atgaaaacat tgatgaccat cgcggtgatc ggagcggcga
     1141 ttatcggtga atggcgtgaa ggtgcagttg tggtcttcct tttcgcggta agtgaagcgc
     1201 tggaagcata ctcgatgaac aaagcacgtc aatccattaa acaattgatg gacattgcac
     1261 cgtctacggc cacagtcctt cgggatggta accaagtgga gctcgatacg gaggatattg
     1321 tcataggcga cacgctcctc gtaaagccgg gccagaaaat tgcgatggat ggcatcgttc
     1381 gtacagggat gtcctctatt aatgaagcac cgattacggg cgagtcgatg ccggcaccga
     1441 agacagttgg cgacgaagtg tttgcaggaa ccgtcaacga agaaggggct ttggaaatcg
     1501 aagtgacgaa actcgttggc gatacaacga tagcgaaaat tattcactta gtggaggaag
     1561 cacaggctga gaaagcgcca acgcaaaaat ttgtggaccg cttcgcaaag tactatacgc
     1621 caatcatcat tgggattgcg tttttagtcg cgatcgtgcc gccgctcttt accggggatt
     1681 ggcacacatg ggtgtaccaa gggctagcgg ttcttgtggt cggttgtcct tgtgcactcg
     1741 tcgtctcaac accggtagcc atcgtgactg caatcgggaa tgccgcacgc catggcgttt
     1801 tgattaaagg cggtgtgcat ttagaacaag ctggacggat caaggcgatt gcctttgata
     1861 aaacagggac gctgacaaag ggatatccag acgtgacaaa cgtcgtttcg ttaaatggaa
     1921 tggacgaaaa agaactcatt agcatcgcag cggcagttga atccatgtcg cagcatccgt
     1981 tggcgaaggc gattctatcg tattcgaaac gaataggagc tccgatcaaa gaagcgacta
     2041 attttcagtc ggtgacaggc aaaggtgcat atgccacggt agaaggcgtg accgtatcga
     2101 ttggcagtat cgattggatg gaggagcttg gagacgtacc tggtactcta cgggaatcgt
     2161 caacttcctt gcaggctgag gggaaaacag tcgtagcagt tgcgttagat ggagtcctag
     2221 cgggaatcat cggtatttct gacccaatcc gctcaactag cgcatccgta ttggaaaagc
     2281 tgaagaacat tggtgtccgt caaacgatta tgttgacggg ggacaatccg aaaactgccc
     2341 aagcaatcgc ggaaaagctg ggcgtcacag acgttcgggc gaaactgatg cctcaagaca
     2401 aattgtctgc aattcttcat atgcgtcaac agtacggtac ggttgccatg gttggagacg
     2461 gaattaatga tgcacctgcc cttgcagctg ctgatgtcgg aatagccatg ggaggagcag
     2521 gaacggatgc cgcgttggag acggcagaca tcgctttaat ggccgatgat ttggaaaagc
     2581 tgccgtatac gatcaaatta agccgaaaag cattgcgtat tattaaagaa aatattatgt
     2641 ttgcattagg cttaaaaatt gtggcgctac ttctcatta
//
LOCUS       DASULI010000082         5910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_989062, whole
            genome shotgun sequence.
ACCESSION   DASULI010000082 DASULI010000000
VERSION     DASULI010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989062"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1036)
                     /locus_tag="VFX34_01830"
     CDS             complement(53..1036)
                     /locus_tag="VFX34_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007543535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX5563673.1"
                     /translation="MTKKKQPPAYGGQALIEGVMFGSRNHTVTAIRRKDDSLDYFYVP
                     KEQKPFSTKLKKIPFVRGIVALIESAGIGSRHLTFANERYDVMPGEEVQDNGEESSKL
                     AMVIGVAAVGVLSFLFGKFAFTLVPVFLAQMLDFIAPGKTAQILLESFFKLTLLLLYI
                     SLISMTPLIKRVFKYHGAEHKVINAYENGLPLTVENVQAQSRLHYRCGSSFLLFTVIV
                     GMFIYFLVPTDPLWLRIVNRILLIPVVIGISFEVLQFTNSLRNIPVLKYLGYPGLWLQ
                     LLTTKEPEDNQVEVAIASFNKLLEIEEQGIENVDILKSTDTKVHDSTVPAN"
     gene            1211..1627
                     /locus_tag="VFX34_01835"
     CDS             1211..1627
                     /locus_tag="VFX34_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563674.1"
                     /translation="MFNKTMIWTTLIATLFTLGALKFLHFFNFIKWSPVGWGKKWLLF
                     GSSDEGWKWALLFIILFIVFGLFYGLSRAAANIPPAITAIVVSIIAAVAIEWIIYSPQ
                     TFMEGVKKLSIPFFSIIAIVCRFITGTAVYMKKTFN"
     gene            1703..2149
                     /gene="aroQ"
                     /locus_tag="VFX34_01840"
     CDS             1703..2149
                     /gene="aroQ"
                     /locus_tag="VFX34_01840"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5563675.1"
                     /translation="MRLLVLNGPNLNRLGKREPGIYGAETLSDVEDHLHSIAKEELVD
                     ISFYQSNVEGALIDKIHEADDTGCDGIIFNPGAYTHTSVALRDAIASVDVPVIEVHIS
                     NIHSREPFRQTSMLAPVCIGQLSGFGTGGYELALRAFLLRRKGVKL"
     gene            2146..3204
                     /locus_tag="VFX34_01845"
     CDS             2146..3204
                     /locus_tag="VFX34_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5563676.1"
                     /translation="MKLAKLREALKEHGLDALLVTNPYNRRYVTGFTGSAGVAVVSAE
                     DAVFITDFRYTEQAAVQVRGFRIVKHEKTIIEEVANQVEQMKIKTLGFEKDNVSFGTY
                     ELYNDKVDADLKAVSGIVEKLRMVKSADEIEILKQAAKIADDAFAHICTFIKPGVTEL
                     EVSNELEMFMRKQGATSSSFDIIVASGERGALPHGVASDKVIQSGEMVTLDFGALYNG
                     YISDITRTVAVGEPSEKMKEIYEVTLTAQELALEKIKPGMTGIEADAIARDYIKSKGY
                     GEAFGHSTGHGIGLEVHELPGLSFRSETVLEPNMAVTVEPGIYLPGIGGVRIEDDILI
                     TEDGNVRLTHSPKELIIL"
     gene            3227..3784
                     /gene="efp"
                     /locus_tag="VFX34_01850"
     CDS             3227..3784
                     /gene="efp"
                     /locus_tag="VFX34_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5563677.1"
                     /translation="MISVNEFKTGLTIEFEGDIWRIIDFQHVKPGKGAAFVRSKLRNL
                     RTGNVNEKTFRAGEKVEKAQIDNRRMQYLYANGDDHVFMDNESYEQIELPSAQIEEEL
                     NYLRENMEIHIVSFKGEILGVELPVTVTLEVTETEPGIKGDTASGGSKPATLETGLVV
                     QVPFFVNVGDKLIINTEEGEYVSRA"
     gene            4124..4333
                     /locus_tag="VFX34_01855"
     CDS             4124..4333
                     /locus_tag="VFX34_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HEX5563678.1"
                     /translation="MELNDLLRIAGIGLVIGFLHVFFEQTGKKEFSFFLFFVAYIYIA
                     AEMLRFLRIFFSEIAEFFQWLSLAF"
     gene            4312..4710
                     /locus_tag="VFX34_01860"
     CDS             4312..4710
                     /locus_tag="VFX34_01860"
                     /inference="COORDINATES: protein motif:HMM:NF018401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HEX5563679.1"
                     /translation="MAFLGILMVTLFQLVVIYLLLLIVSFASKSLQPIIYAAIFFFFL
                     SYVGMTIIIPFGKTFIHLFEPLPGSYAKLLIGSVFLFYFSELIAKHVAEAGYESIATV
                     AQLAIKIAILSLWLPQLSMLLDTLSKFIIK"
     gene            4726..5625
                     /locus_tag="VFX34_01865"
     CDS             4726..5625
                     /locus_tag="VFX34_01865"
                     /inference="COORDINATES: protein motif:HMM:NF021088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HEX5563680.1"
                     /translation="MMPMLESVIGTVLASFIIVIISTFMALVLDFLFPSFSRWTRTFL
                     FFIVLTVVLYPAYEHLQMIRSITQTVATMFISIYPLLTASIIASGGSFSMLNFQPAML
                     LFANGAVILSDKILIPMLSAAMLFDIVSRFHPAIPFTKMADLLRGSLIGLVSALVAAY
                     SIFITAGGTISWALTGVTSEPIKKLISQNIPLVGSFMTDSLGAIGRYSSGASVFIGAW
                     LIVTIWSVALLPTLKTLLTGFLYRWTAAIIEPFANEEITGILDDIGKTLFVLCAVSFL
                     VAFAFIYTALFTVILVKLITVVK"
     gene            5634..5801
                     /locus_tag="VFX34_01870"
     CDS             5634..5801
                     /locus_tag="VFX34_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563681.1"
                     /translation="MLEFLTGVLLITVLSSVLLLLLPEDKEEEMLPLVKMAMGIWIIH
                     SITRIFGHSLF"
BASE COUNT         1634 a         1283 c         1350 g         1643 t
ORIGIN      
        1 caataaaaag gctgccccaa aagtcgtttt gactttctgg gcagcctttt tttcaatttg
       61 caggaactgt cgaatcgtgt accttcgtgt cggtcgactt caaaatatcg acattttcaa
      121 ttccttgctc ctcaatttca agaagtttat tgaaagaagc aatcgccact tccacctggt
      181 tatcttcagg ttcctttgtt gtcagcaatt gtagccataa tcccggataa cctaaatact
      241 tcagaacggg gatgttacga aggctgttcg taaactgtaa cacttcaaaa gaaatgccga
      301 tgacaacggg aatcaataga atccggttga caatcctcaa ccagagagga tctgtcggga
      361 ccaagaagta aatgaacatg ccgacaatta ccgtaaacag caagaaactg cttccgcatc
      421 tataatgcag gcgggactgc gcttgaacat tttcaacggt aagtggaagt ccgttttcat
      481 aagcgttgat cactttatgc tcggcaccgt gatatttgaa tacccgttta atcaagggtg
      541 tcatggagat gagggaaatg tacaacagta gtaatgtaag tttgaaaaag ctctccagta
      601 aaatctgtgc cgtctttcca ggcgcgatga aatctagcat ttgcgccaaa aagacgggca
      661 ctagcgtaaa cgcgaacttg ccgaataaaa atgatagaac gccgaccgct gcgaccccga
      721 tgaccatggc cagcttggag ctctcttcgc cattgtcttg cacttcttct cctggcatca
      781 catcataacg ctcattcgca aacgtcagat gtctggatcc gatgccggcg gattcgatta
      841 aagcgacaat ccctcgaacg aaaggtatct ttttcaattt cgtcgaaaat ggcttctgtt
      901 ctttcggaac ataaaaataa tcgagagaat catctttgcg ccggatggct gtcactgtat
      961 ggtttctgct gccgaacata acaccttcga tgagcgcctg cccaccgtat gccggcggtt
     1021 gctttttttt agtcatgggt accaacacct tctcttatca aatgaaaagc aaaatgtgaa
     1081 ttatgatatt gtcaattaca ttataacaaa taacttatct tactattgta caaaaaatcg
     1141 gttgtcgact ccaccattaa ggtttaaaaa agggcctatt tcccgacact cattgtaagg
     1201 aggggtcgag atgtttaaca aaacaatgat atggacaaca ttaatcgcaa ctttattcac
     1261 attgggagct ttaaagttct tgcatttttt caatttcata aaatggtcgc ctgtcggttg
     1321 gggcaaaaaa tggctattat tcggatcatc cgatgaggga tggaaatggg cactcctatt
     1381 catcatcctc tttattgttt ttggtctatt ctatgggctg tcaagggccg cggcaaatat
     1441 cccgcccgca attacagcta tcgttgtcag cattattgca gccgttgcaa tcgagtggat
     1501 catctattct ccacagactt tcatggaggg tgtcaagaaa ttgtccattc ccttcttttc
     1561 aatcatcgct atcgtctgca gattcatcac agggaccgct gtttatatga agaaaacttt
     1621 caattgacaa ctataaatag gttgcgccat tggaacgata tgataaaatg gggaagaaaa
     1681 tggtgagggg aatggtgtgg ctgtgagact gcttgttctg aatggaccga atttgaatcg
     1741 gctaggcaaa agggaacctg ggatctatgg cgcagaaaca ttatcggatg ttgaagacca
     1801 tctgcactct attgcgaaag aggagctcgt tgacatttct ttttatcagt cgaatgtaga
     1861 aggcgcgttg atcgataaaa tccatgaagc cgatgataca ggctgtgacg gtatcatttt
     1921 caatcccgga gcttatacac acacaagtgt tgcattaagg gatgcaatcg cttctgtcga
     1981 tgttccggtc attgaagttc atatttcgaa tatacatagt cgggaaccgt tcagacaaac
     2041 ttcaatgctc gctccggtgt gcataggaca attgtccgga tttggcacag gcggttatga
     2101 gcttgcactc cgagctttcc ttctccgaag aaaaggggtt aagctatgaa actggcaaaa
     2161 ttgcgggaag cattgaaaga acacggatta gatgcattat tggtgacgaa tccatacaac
     2221 agacgctatg tgaccggatt tacaggaagt gcaggggtag cggtagtttc agccgaggac
     2281 gcagtattca tcaccgattt ccggtatacg gaacaggctg cagtacaagt gcggggcttc
     2341 cggatcgtca agcatgaaaa aacgatcatc gaggaagttg caaaccaagt ggaacagatg
     2401 aaaatcaaaa cattaggctt tgaaaaagat aatgtttcat ttggcacgta cgaattatat
     2461 aatgacaaag tggatgcgga tcttaaagcg gtttccggca tcgtcgaaaa gcttcgtatg
     2521 gtgaagtcag ctgatgaaat cgaaatcctg aaacaagcgg cgaaaattgc ggatgatgcg
     2581 tttgcacata tttgcacatt catcaaacca ggcgtaactg agcttgaagt atcaaatgaa
     2641 ctcgaaatgt tcatgagaaa acaaggggcg acttcctctt ccttcgatat cattgtagct
     2701 tcaggggaac gcggagcatt gccgcatggc gtcgcttcag acaaagtgat ccaatccggc
     2761 gagatggtca cattggactt tggggcactc tataatggat atatttcgga catcacccgt
     2821 acagtcgcgg taggcgaacc ttccgaaaaa atgaaagaga tttacgaagt gacacttaca
     2881 gcgcaagaac tggctttaga aaagatcaaa ccgggtatga ctggaataga agcagatgca
     2941 attgcccgtg actacatcaa atctaaagga tatggggaag ctttcggaca ctcgactggc
     3001 cacggaatcg gcttggaagt acatgagttg ccgggattat cattccgttc tgaaactgtt
     3061 cttgaaccaa atatggcagt cacagttgaa ccgggcatct acttgcctgg gatcggcggt
     3121 gtccgcatcg aggatgatat actgatcacg gaagacggca atgtccgttt gacacattca
     3181 ccaaaagaat taatcatcct ataacgaaca taatggagga aaatgcatga tttctgtaaa
     3241 cgaatttaaa acagggctaa caattgaatt tgaaggggac atttggcgca tcatcgattt
     3301 ccaacacgtc aagccaggaa aaggtgcggc attcgtccgt tcaaaactac gtaatctgcg
     3361 tacaggaaat gtgaatgaaa agactttccg tgcgggcgag aaagttgaaa aggcacaaat
     3421 tgacaaccgt cgtatgcaat acttatatgc aaacggcgat gatcatgtat tcatggacaa
     3481 tgaatcatac gagcagatcg agttgccttc agcacaaatc gaagaggaat tgaactattt
     3541 gagagaaaat atggaaatcc atatcgtctc attcaaaggc gaaatccttg gcgtggaact
     3601 tccggtcacc gtcacactcg aagtgactga aactgaacca ggcatcaaag gcgatacggc
     3661 aagcggcgga tcgaagccgg ctacactcga aactggcctc gtcgtccaag ttccattctt
     3721 cgtaaacgtc ggcgataaat tgatcatcaa cacagaagaa ggcgaatacg tctcaagagc
     3781 ataataaagt gtaatactcc aactcttaac agggttgggg ttttttattt tccagtatgg
     3841 attgtagggg aaggcggcga ctcctggggg attagcgaac gtcgttacga agaacggcgt
     3901 ttgcgagcaa aagcaaagcg ttgcgagcag gaatggcaga acggatgcct taatttctgc
     3961 aaaggacgca gtaatacggc aaatcgaact cttcgtggtt cgattggctc accgcctgcc
     4021 cccggaaagc gtccgcctga agcgtaaatc catttttctc tatattactc catcatattt
     4081 cccctcaccc tctcataagt tgttcatgaa gggggacccc gaattggaat tgaatgattt
     4141 actgcgaatc gccggaattg ggttggtcat cggctttcta catgtctttt ttgaacagac
     4201 cggtaagaag gagttctcat ttttcctttt ctttgtagcg tacatttaca ttgctgccga
     4261 aatgctccgt ttccttagga ttttcttttc ggaaattgcc gagttctttc aatggctttc
     4321 cttggcattc taatggttac gcttttccaa ctggttgtca tctaccttct tctgcttatc
     4381 gtgtccttcg cttccaagag cttgcaaccg atcatatacg cagctatatt cttcttcttt
     4441 ctgtcgtatg tcggtatgac aatcatcata cctttcggaa aaaccttcat acatttattc
     4501 gaaccgttgc cgggatccta tgcgaagctt ttgatcggaa gcgtcttttt gttttatttc
     4561 tcggaattga ttgcaaagca cgttgccgaa gcagggtatg aatcaatagc aacggttgcg
     4621 cagttggcta ttaaaatagc aattctatcg ctctggcttc cacagttgtc aatgctgctc
     4681 gacacgcttt ccaaatttat cattaaatga tcggatgaag aatccatgat gcctatgcta
     4741 gaatccgtaa tcgggacagt cctcgccagt ttcattattg tcatcatttc aacattcatg
     4801 gcgttggtgc tcgatttcct ttttccttca ttcagcagat ggacgagaac cttcctgttt
     4861 ttcatcgttc tgactgtcgt cctttaccct gcgtatgagc atttgcaaat gatacgatct
     4921 ataacacaga cagttgcaac gatgttcatt tccatttatc cgttattgac tgcaagcatc
     4981 atagcatccg gcggctcctt cagcatgctt aactttcagc ctgccatgct gttgtttgca
     5041 aacggcgcag ttatcctctc ggacaagatt ctcataccta tgttgtccgc agcgatgctg
     5101 tttgacatcg tttcaagatt ccaccccgca attcctttta caaagatggc agatttgctg
     5161 agggggtccc tgatcggcct ggtttcagcc ctggttgcgg cctactccat cttcattact
     5221 gcaggcggca cgatttcgtg ggcattgacg ggtgtaacga gtgagccgat taaaaagttg
     5281 atcagtcaaa atatcccgct tgtcggctct ttcatgacgg atagtttagg agcgatcggg
     5341 cgctattcat caggcgcaag cgtttttatc ggggcgtggc tcatcgtcac aatctggtcg
     5401 gtcgccctat tgccaacgct aaagacgcta ttgaccggtt ttttatatcg atggacagca
     5461 gctatcatcg agccgtttgc gaatgaggaa ataacaggca tactcgatga tatcggaaag
     5521 acgttattcg ttctttgcgc agtctctttc ctcgtcgcgt ttgctttcat ctatacagcc
     5581 ctgtttactg tcatcctcgt caaattgata accgtggtga aatgaggggc gatatgctgg
     5641 agtttttgac gggcgtgtta ctcataactg tactatcctc agttttatta ttattgttgc
     5701 cggaagacaa ggaggaggaa atgctgccgc tcgtaaagat ggcgatggga atttggatca
     5761 ttcattccat cacgagaatt tttggtcata gcctcttctg aacaagcata atatgcatca
     5821 tagataacac gaaccatatc cgtcttgaag ggatgaaatg gtgatcatat gcaaaaatca
     5881 aaacgaaaaa ttcatatgct cttcatcggg
//
LOCUS       DASULI010000083         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1125648,
            whole genome shotgun sequence.
ACCESSION   DASULI010000083 DASULI010000000
VERSION     DASULI010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125648"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFX34_01875"
     CDS             complement(<1..893)
                     /locus_tag="VFX34_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563682.1"
                     /translation="MAKKELLKVEGLKQYFPIKGGMMGRTVNYVKAVDDISFTVYEGE
                     TVSIVGESGCGKSTTGRAILRLEEPTEGTVTFNGEELTSLSKRKMRKHRKDLQIIFQD
                     PYASINPRQTVANVLDEAMVIQKVIKSPKERRKRIIELLETVGLNEFQADRYPHEFSG
                     GQRQRIGIARALSVNPKLIICDEAVSALDVSIQAQVLNLLEELQQDYKLTYLFISHDL
                     GVVRHISDRVIVMYLGKIVEIGDKKSIFNSPKHPYTKALLSAIPVPDPTREKKHIVLK
                     GDVPSPIDPPTGCRFHTRCPFA"
     gene            complement(838..1890)
                     /locus_tag="VFX34_01880"
     CDS             complement(838..1890)
                     /locus_tag="VFX34_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563683.1"
                     /translation="METPLLEVRDLCTSFYTDEGEVKAVDGVSFTLPKGKTLGIVGES
                     GSGKSITALSILKLLAANGKIIGGEVNYKGENLVDYNDKKMRDIRGNAISMIFQEPMT
                     SLNPVFTVGQQISESLIKHQKLSKKEAIVRSVELLKLVGIPSPEKRVKQYPYELSGGM
                     RQRVMIAIALACNPEVLIADEPTTALDVTIQAQILGLIRDLQQRLGMSVIMITHDLGV
                     VAETCDYVAVMYAGQVVEYSDVVTLFQKPKHPYTIGLLNSLPRHDVEVEKLEPIRGNV
                     PNLHNMPVGCRFAPRCPAATDLCRNELPDLLKDREGNDVRCWIYSEKWPGESEVIVYG
                     EKRIAEGRGSETVLSD"
     gene            complement(1918..>2466)
                     /locus_tag="VFX34_01885"
     CDS             complement(1918..>2466)
                     /locus_tag="VFX34_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006207784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563684.1"
                     /translation="IMIVALFGLSMPNFWFGLMLIQWFVIGPEWMPDFMKMRATGWGD
                     DWRQMVLPVITLGTGGAAIIARMTRSSMLEVIGQDYIRTARAKGMSERVVVYRHALKN
                     ALIPVITVVGLEFGSFLGGAVLTESIFAINGMGRLTIDAIRQRDFPIVQGTVLVISFL
                     FVFVNLLVDISYRLLNKRIDLN"
BASE COUNT          666 a          623 c          494 g          683 t
ORIGIN      
        1 gcaaatgggc agcgcgtatg gaagcggcag cctgtcggtg gatcgattgg cgatggcaca
       61 tcacctttca gaacgatgtg tttcttctca cgtgtcggat ccggtacagg aattgccgag
      121 agcaaagctt ttgtataagg atgcttcgga ctattgaaaa tggatttttt gtcaccaatc
      181 tctacgatct ttccaaggta catgacaatc acccggtccg aaatatgacg gacaaccccg
      241 aggtcatggg agatgaaaag ataggttaat ttataatcct gttgaagctc ctctaacaag
      301 tttaatactt gggcctggat ggagacgtcc agagcggaaa cggcctcgtc acaaatgatg
      361 agctttggat tcaccgaaag tgcacgggca attccgatcc gctgacgctg tccaccagag
      421 aattcgtgtg gatatcggtc tgcttgaaat tcattcaaac caaccgtttc gagtagttca
      481 ataatccgct ttcttcgttc ttttggggat ttaatgactt tctgtatgac cattgcttca
      541 tctaacacat tcgcaaccgt ttgacgtggg ttgattgatg catatgggtc ttggaaaatg
      601 atttgaagat cttttcgatg ctttctcatt ttgcgttttg agagcgacgt caactcttcc
      661 ccattaaagg ttacggttcc ttccgtcggc tcttccaatc gcagaatcgc acgacctgtc
      721 gtagattttc cacaaccaga ttctcctacg atactaacgg tttcaccttc atataccgta
      781 aaactaatat cgtctacagc tttcacatag ttgactgtcc ggcccatcat tccgccttta
      841 atcggaaagt actgtttcag accctcgacc ttcagcaatt cttttttcgc catagacaat
      901 cacctccgat tcaccaggcc acttctctga ataaatccag cagcggacat catttccttc
      961 tcgatccttc aataaatcag gcaattcatt tctgcataag tctgtggcag ccgggcaacg
     1021 cggggcaaat cggcaaccga ccggcatatt atgcaggttt ggaacattcc cacgaatcgg
     1081 ctccagcttt tcaacctcca cgtcatgacg tggcaaagaa tttagcaatc cgattgtgta
     1141 cggatgcttc ggcttttgga aaagcgtcac aacatcggag tattctacaa cctgtccagc
     1201 gtacataacg gctacgtagt cacatgtttc agcaacaaca cccaagtcat gagtgatcat
     1261 aattacggac ataccgagac gctgctgtaa atcacgaatc aaacctagaa tctgcgcttg
     1321 gatcgtcacg tctaacgctg tcgtcggttc atcggcaata agaacttccg gattacatgc
     1381 taacgcaatc gcaatcatga cacgttgccg cattccgccc gataattcgt acggatattg
     1441 ttttacacgc ttttcaggtg atggaatccc gactagcttc aataactcaa ccgatcggac
     1501 gatcgcttcc tttttcgaca acttttggtg tttaataaga gattcactga tttgttggcc
     1561 aaccgtaaat acagggttca atgatgtcat cggctcctga aatatcattg aaattgcatt
     1621 tccgcgaatg tcccgcattt ttttatcatt gtaatcaact aggttttcac ctttataatt
     1681 aacttcaccg ccaatgattt tcccgttcgc agctagtagc tttagaatgg ataatgccgt
     1741 aatactctta cctgaaccgg attcaccaac gattccaagt gtctttcctt ttggtaaagt
     1801 aaagcttaca ccatcaactg ccttcacttc cccctcgtct gtataaaagg atgtacagag
     1861 atcacgtact tccaacaatg gagtttccat agcggacacc tctctttctg atctttatca
     1921 gttcaagtca atccgcttat tcagcaagcg gtatgaaata tcaacgagca agttaacaaa
     1981 aacgaacagg aatgaaatga ctaataccgt accttgaaca atagggaaat cccgttgccg
     2041 gattgcatcg attgtcaaac ggcccatccc gttaatcgca aagatcgatt cggttaaaac
     2101 cgctccccct aggaacgaac caaactcaag accaacaacc gtaataaccg gaattaacgc
     2161 atttttgagt gcatggcggt atactacaac ccgttcgctc attccctttg cacgcgctgt
     2221 ccggatataa tcctgtccga tgacttcaag catcgaagaa cgagtcatcc gggcaataat
     2281 cgccgctcct ccggttccta acgtgataac cgggagaacc atctgcctcc aatcatctcc
     2341 ccaaccggtt gctctcattt tcatgaagtc gggcatccat tcaggtccga ttacgaacca
     2401 ttggattagc atgagtccaa accagaagtt aggcattgat aatccaaata gggcgacaat
     2461 cataat
//
LOCUS       DASULI010000084         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1304149,
            whole genome shotgun sequence.
ACCESSION   DASULI010000084 DASULI010000000
VERSION     DASULI010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304149"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFX34_01890"
     CDS             complement(<1..794)
                     /locus_tag="VFX34_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX5563685.1"
                     /translation="MASYESLLMIPEWRFWDESISGINRSALESFAADDTLCHLVGQG
                     MSIPTVRTWVHDKTVVLGIQDHRLPYVAEAMELCERAGYTPIVRNSGGLAVVLDPGVL
                     NISVVLSEKGTSIDIPTGYEVMLSFVKLLFPEVAEQIEAYEIVGSYCPGSYDLSIGGK
                     KFAGISQRRLRQGIAVQVYLCIEGSGAERAELIRDFYEIGLKGEKTKFAYPSIKPEVM
                     ASMSELLGMELSVHDVVIRVQHTLHSLSESIKRGGIQPDELELYTF"
     gene            complement(798..1778)
                     /gene="pta"
                     /locus_tag="VFX34_01895"
     CDS             complement(798..1778)
                     /gene="pta"
                     /locus_tag="VFX34_01895"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX5563686.1"
                     /translation="MADFFKSIKEKVKGTEKTIVFPEGTDPRILSAADRLYREELLKP
                     ILLGDEQVLQAAAKAGVDLTGIERINPAEAPYFDELVSTFVDRRKGRVTTEQARTMLL
                     DVNYFGTMLVYTGRADGLVSGAAHTTADTVRPALQIIKTKPGVSKTSGAFIMVKGEER
                     LVFADCAITVDPTAQELAEIAVESSKTAKAFGINPRVAMLSFSTKGSAVTEETNKVSR
                     AVEMAKVMDPKVVLDGEFQFDAAYVPDVAAKKAPDSVIQGDANVFIFPTLEAGNIGYK
                     IAQRLGSYEAIGPLLQGLNAPVNDLSRGCSADDVYKLALLTAAQSITSGE"
     gene            1956..>2053
                     /locus_tag="VFX34_01900"
     CDS             1956..>2053
                     /locus_tag="VFX34_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006096706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HEX5563687.1"
                     /translation="MNEAAITLDGWYVLHDLRSMDWASWKQISNEER"
BASE COUNT          523 a          534 c          442 g          554 t
ORIGIN      
        1 taaaacgtat acagttcgag ttcatcgggt tggattcctc cacgtttgat tgattcggat
       61 aaggaatgaa gcgtatgttg tacccggatg acaacatcgt gtacagagag ttccattcca
      121 agcaattccg acatcgaagc cattacttcc ggcttgatgg acgggtatgc gaatttcgtt
      181 ttttcgccct ttagcccgat ttcataaaag tcccgaatca attcagcccg ctccgctccg
      241 cttccttcaa tgcataaata cacttggaca gcgattccct ggcgcagccg ccgttgggaa
      301 atccccgcaa actttttccc gccaatgctt aaatcatacg agcctggaca ataagagccg
      361 acaatctcat acgcctctat ttgttcggca acctcaggaa ataggagttt tacgaatgac
      421 agcattacct cgtatcctgt tggaatatcg attgacgttc ctttttcgga aaggacaact
      481 gaaatattta gtacacctgg gtccagaacc acggccaatc cgccagagtt ccggacaatc
      541 ggagtatagc ccgcacgctc gcacagttcc atcgcttctg cgacatacgg taaacggtga
      601 tcttgaatgc caagtacaac cgttttgtca tggacccatg tccgaacggt cggtatactc
      661 atcccttgtc caacaaggtg gcaaagggta tcgtctgccg caaatgattc aagggcagac
      721 cggttaattc cactgattga ttcatcccaa aaacgccatt cgggaatcat caacaacgat
      781 tcatatgaag ccatgtttta ctctcctgaa gttatacttt gagctgctgt taaaagagcg
      841 agcttgtaga cgtcatcggc agaacatccg cgtgacaaat cattgacggg tgcgttcaaa
      901 ccttgtagga gtgggccgat tgcctcgtag ctgcctaggc gctgcgcaat cttgtatcca
      961 atgttccccg cttccaacgt cggaaaaatg aagacattgg catctccttg aatgactgaa
     1021 tccggtgcct ttttcgccgc gacatccggt acatacgcag catcaaactg aaattctccg
     1081 tctagaacga ctttcggatc catcactttt gccatctcaa ctgcacgtga gaccttattt
     1141 gtctcttccg taacagccga cccttttgtt gaaaacgaaa gcatggcaac gcgagggttg
     1201 attccaaacg ctttggcagt ttttgaggat tctacagcga tttccgccaa ctcttgtgca
     1261 gttggatcta ctgtaatggc gcagtcggca aacaccaacc gctcctcccc cttgaccata
     1321 ataaacgcgc cactcgtctt ggaaacaccg ggttttgttt taatgatttg aagagctggc
     1381 cggacggtat ccgctgtcgt atgagccgct ccgcttacaa gtccatccgc tcgccctgtg
     1441 tatacaagca tcgtgccaaa ataatttaca tccaatagca ttgttctcgc ctgttcagtt
     1501 gtcacccttc cctttcttcg atcaacaaat gtcgaaacaa gttcgtcgaa ataaggggct
     1561 tccgccggat taattcgttc aatgccagtt aaatcaacac cggcttttgc agccgcttgg
     1621 agtacttgct catctccaag gaggatgggc ttgaggagtt cttcccgata cagtcgatct
     1681 gcagcagaaa ggatacgagg atccgtacct tctggaaaca caatcgtttt ttcggtcccc
     1741 ttcacttttt ctttaatact cttaaagaaa tcagccattt atacatccgc cttccgtcta
     1801 ttcttgtcta ttcacagcat acacgatgtg aacctcacct gaaaagggag gcgcaccttg
     1861 acgaatttgt ggcaagacgc ttctcatcag gccttttgtc atcaggtatg ctacactaat
     1921 gtcgatgaat atttacatgg aggagtgact cgttcatgaa cgaagcagca atcacgctcg
     1981 acggctggta cgtattacat gatttacgat caatggactg ggcttcttgg aagcaaatct
     2041 ccaatgaaga acg
//
LOCUS       DASULI010000085         9429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1134050,
            whole genome shotgun sequence.
ACCESSION   DASULI010000085 DASULI010000000
VERSION     DASULI010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134050"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFX34_01905"
     CDS             <1..314
                     /locus_tag="VFX34_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEX5563688.1"
                     /translation="SILRKAGGSNTFAIDEVNDHEWDIVKQLDQFPQAVKRANDELDP
                     SVIAKYAVDLAQAFNSFYGNVQVLSDETHKHYRVALTECVIIVLKESLRLLGMKAPDE
                     M"
     gene            complement(370..1764)
                     /locus_tag="VFX34_01910"
     CDS             complement(370..1764)
                     /locus_tag="VFX34_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HEX5563689.1"
                     /translation="MDSVGVVEWLVGPANDFIWTYILIGLLLLIGLYFTIRTKFVQFR
                     LFGEMFRLIFEKREGNRGVSAFQAFTISAASRVGTGNVTGVALAIGIGGPGAVFWMWV
                     IAIIGMATAFIESALAQVYKVRDGDTFRGGPAYYMEKALGLRKLGIVFAILLTMCFGF
                     IFNSVQSNTISQSFHDVFNLPYWVVGLSLVVLTGIIIFGGVQRIVKVTQSIVPIMAVF
                     YLLIAIYVVAINITEVPAMIALIFEHAFGIKEMAGGGIGAAMLQGIRRGLFSNEAGMG
                     SVPNAAATASATHPAKQGLVQSLGVFFDTIIICSATAFIIILAGLYDKGEESGILLTQ
                     SSMAVHVGSWAPYFVAIAIMFFAFSSIVGNYYYGETNIEFINTHKSWMTLYRIAVLAM
                     VMFGALAKVDIVWSMADLFMGLMAIINLLVILALGKVAFHVLEDYTKQRKQGKNPVFK
                     ADPSLKGAECWKEE"
     gene            2496..3641
                     /locus_tag="VFX34_01915"
     CDS             2496..3641
                     /locus_tag="VFX34_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829693.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5563690.1"
                     /translation="MLKFENVSKVYEDGFKAVDSVSFEIPEGELLVLIGPSGSGKSTT
                     MKMINRMQPHTSGKISINGKDNKSYVASDLRRGIGYVIQQIGLFPHYTIEKNIAIVPE
                     LNGWSQDKIKPRVTELLELVGLDPEIYASRYPKELSGGQQQRVGIARALASDPEIILM
                     DEPFSALDPITREQLQGELISLQKKLNKTIIFVTHDMDEALKMGDRIAIMKDGKLLQL
                     DTPEKLLHEPAHGFVEEFIGKHRIIQNPELMPVLDIMSESVVTTLPQSSPEKAISLIR
                     QRKITDLIIVDESKELLGIVSAYDVIKKVNDIRTIAEIMQPIGYFLEDTASAKDAIIM
                     MDETPFGLIPIVDEKTRVVGLVTRGSLLSALSSQWTETETAAEVVAI"
     gene            3638..4297
                     /locus_tag="VFX34_01920"
     CDS             3638..4297
                     /locus_tag="VFX34_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563691.1"
                     /translation="MNNLSIWQQLTEQSQMRWREVLEATSVHIQLVFFSMLIAIILGV
                     SLGILVTRVPQLTTLVLGGTGVMQTIPSLALLGFMIPIFGIGVKTAIAALFLYSLLPI
                     ARNTYAGITEVDPATREAAKGMGMTSAQILLKVELPLAIPVIMAGIRTAAVINVGNAT
                     LAAFIGAGGLGDFIFLGITRGIDGLILLGAIPAALLAIILEVFFSSVERWTTPKGLKT
                     E"
     gene            4294..5190
                     /locus_tag="VFX34_01925"
     CDS             4294..5190
                     /locus_tag="VFX34_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5563692.1"
                     /translation="MRKKFIGFLLIAVVASMLSGCIFIEKDSLILGSRNNTESIILSH
                     VMGQLIENKTDIKVIYKENLGGSNVVWNAMLNGNIDVVPDYTGTIVVNYYHEEPGNAE
                     ETLELTRRLVGEDGIIAFNTFGFNNTYTLALDESKAEELGVVTFSDFAAYSEDFTLGA
                     VFEFIDRPDGLPGFQKEYGLKFKNVKGMDHGIMYRSISAGEVDVINSYTTDGQLQEFD
                     LRVLEDDKSYFPPYHALPLVRKETLKEYPEIEAVLRQLEGAIDEEAMQKMNAKVDNDG
                     MMVERVAEEFLVESGLIEGVAR"
     gene            complement(5519..6436)
                     /locus_tag="VFX34_01930"
     CDS             complement(5519..6436)
                     /locus_tag="VFX34_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX5563693.1"
                     /translation="MRRLPDHHEAIPALRSKHAAVQAGFGGEQELDKVFESYAFSMKH
                     GIFHDLSLASSTYFQVDTLFITPWYAVLFEVKNIAGELTVTENPPQLIRVLDSGQVSG
                     FKSPIAQLESNCELFQDWLYSRNIALPVYGAVVLAYAKQRIDVFDTNIPILFPSAVPS
                     FIRKLPTTSPLLDDETFVDLMMQLGSSHREFIPSSICETYSIRHSDIRTGVICPGCGF
                     VGMVRHSRSWHCTTCGIADREAHKQAIRDWFMLFGGKMSNKDCRAFLHIEHQQTAHRI
                     LQGMGLHTEGANRNRTYTMQFLPKNKKRT"
     gene            complement(6553..6906)
                     /locus_tag="VFX34_01935"
     CDS             complement(6553..6906)
                     /locus_tag="VFX34_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5563694.1"
                     /translation="MTQVCVIGIHVPDMQEAIEFYTGVLGFEVNKEYGPKIVTLVHGE
                     LPIVLEETENAGVNVGSSGIVLTLKTDDIHQSLSKLRENGVELIIAEPADCPPGKYIS
                     FRDPFGNVWEYLEFG"
     gene            complement(6903..7688)
                     /locus_tag="VFX34_01940"
     CDS             complement(6903..7688)
                     /locus_tag="VFX34_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5563695.1"
                     /translation="MLPQELAVEKIAASLKKDPLVQAIFLKGSMGRGEHDAHSDVDLY
                     CLVNEEDKKDFLAARVAHLQAYRDIILLDDIFIVAPQIIAVFDDLLHIDLFTVTKESF
                     VGGDHFKVLYDPKGLMDEFMETLGLELKEEEYRDDVMDVGWFLFQYSKTAARGNDIWA
                     VKMLTNVVHHLGRVLLYRYAPERAKLGLKTIERSLPESVVLELTEILEFVTPGEHVQA
                     VGRIGRLLEREYEWIRMQWSEGSQQMRFLRRMIDQYKGQGELV"
     gene            complement(7783..8070)
                     /locus_tag="VFX34_01945"
     CDS             complement(7783..8070)
                     /locus_tag="VFX34_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563696.1"
                     /translation="MKKRYMIGGVLLFILLIALVTNPSEKEYLQFSEGKYGPPPPSVK
                     MEIERVNFLLFSTYTPVYHVEYGITHLGAFGMFFQLSDGQFDYPIWLDLFN"
     gene            8391..8999
                     /locus_tag="VFX34_01950"
     CDS             8391..8999
                     /locus_tag="VFX34_01950"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5563697.1"
                     /translation="MTPFCPESWPEDPTPASQVNCQECGLYQQGTRMVWGEGNPKAPI
                     MVILDNPGAREDREGNPMVCGTRQTLQQAVYEAGLDKEQIYVTYILKRRPIRKYDKEQ
                     TREICMHRHLDGQLKQQKPDLILCMGNVAVQSFFENAEVDVKGLRGSWHNIKGYPTTV
                     AYHPLAVRRRPNLYKLFLEDIMLVKSKIEDAHVKKGSYHADH"
     gene            8986..>9429
                     /locus_tag="VFX34_01955"
     CDS             8986..>9429
                     /locus_tag="VFX34_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5563698.1"
                     /translation="MPTIEHEISINAPIQICFDVARTVEVHEGKTMLTKQITISGVTK
                     GLMEHGDSVTWQTTHLGIKQKLTSQIIKMVKPYHFTDAMVQGVFQSFTHTHEFIESCA
                     ETIMKDTFTYQSPFGIIGKAADQLFLERYMRNFIALHAKKVKKAAE"
BASE COUNT         2658 a         2428 c         1985 g         2358 t
ORIGIN      
        1 actccatttt gcggaaagct ggaggatcca acacgttcgc gattgacgaa gtgaatgatc
       61 atgaatggga catcgtcaaa cagcttgacc aattcccaca agccgtcaag cgcgcaaacg
      121 atgaattgga tccttccgtc atcgcaaaat atgcggtcga ccttgcccaa gcgttcaact
      181 cgttttacgg aaatgtgcaa gtcctatccg atgaaacgca caagcattac cgtgttgcac
      241 tgaccgaatg cgtcatcatc gtattgaagg aatcattgcg cctgctcggc atgaaggctc
      301 cggatgagat gtaaactaga cgaaaaaagc aatcgttcca cctacaggag gcgggacgat
      361 tgcttttctt cattcttcct tccaacattc agcgcctttt aaactaggat ccgctttaaa
      421 cacggggttt ttcccttgtt ttcgttgttt cgtataatct tccaacacat ggaatgcaac
      481 ttttccaagt gccaagatga cgaggagatt tataattgcc atcaatccca tgaacaagtc
      541 cgccatgctc cagacgatat ccacttttgc aagggcaccg aacatgacca tggcgagaac
      601 cgcaattcgg taaagcgtca tccacgactt atgtgtgttg atgaattcaa tattcgtttc
      661 accgtaatag taattcccaa cgattgagct aaacgcgaag aacataatcg cgattgcgac
      721 aaaataaggc gcccaagaac caacgtgaac agccatcgaa gattgtgtca gcaaaatgcc
      781 gctctcttcc cctttatcat acagacctgc aaggatgatg atgaaggcag tcgctgaaca
      841 aatgatgatc gtatcgaaaa atacgccaag gctttggaca agcccttgct tcgccggatg
      901 cgttgcactc gccgtcgccg cagcgttcgg cacacttccc ataccagctt cattggagaa
      961 taaaccacgt cgaatacctt gcaacatggc agcacctata ccgccgccag ccatttcctt
     1021 aatgccgaaa gcatgctcaa aaatgagtgc aatcattgca ggaacttctg taatattaat
     1081 ggcaacaacg taaatcgcaa taagcaaata aaacactgcc ataatcggaa caattgattg
     1141 tgtcacttta acaatccgct gcacgccgcc gaaaataata attcctgtca gcacgacaag
     1201 agacagtccg acaacccaat acggaagatt gaatacatca tggaacgatt gactgatggt
     1261 gttcgattga actgagttga agatgaaacc gaaacacatt gtgaggagaa tcgcgaaaac
     1321 gatgccgagc ttccgaaggc ccagcgcctt ctccatataa taagccggcc ctccgcggaa
     1381 tgtgtcgccg tctcgaactt tgtaaacttg tgcaagcgca ctttcaataa acgctgtcgc
     1441 catgccgata atggcgatga cccacatcca aaagacagca ccgggtccgc caatcccgat
     1501 cgccagcgca accccagtta cgttccctgt accgacacga gaagctgcac tgattgtaaa
     1561 cgcctggaac gctgaaaccc cacggttgcc ctctcttttc tcaaaaatga gtctgaacat
     1621 ctctccaaat aaacggaact gaacaaattt cgtccgaatc gtaaaataaa gaccgatgag
     1681 caataaaagt ccgatcaata tgtatgtcca aataaaatca ttcgccggtc cgacgagcca
     1741 ctctactact cccacactat ccaccccttc ttcttcatcg taactcacta actgtcttta
     1801 cccagtcgtg cagaataaaa ctcacatttc aaaaatagaa aaaccgctga gccaacatgg
     1861 actcagacgg tctagaagcc atcgtccact ttcgtgaaag cgcttcatat ttattctgat
     1921 ttgattattt tatcatcgac tccgtatttt tcaacctatt ttccgaaaaa aagtatcgaa
     1981 tattgtcgga aaatattggt tatcgcccac ttagggcgat ttatcaccga ctctcgggag
     2041 tttatcaccg acccggcatt ttttactaaa aactgcaagg gtccgaatga tttgcctcga
     2101 caaatcctag cacaaatagt aggactgtag actcattttc ataggtgttt tacattgcca
     2161 ccgactctaa gtactttata accgacttgg tcgagtttat caccgacttt caacgcttta
     2221 tcaccgactc tgcctggctt ataaccgctt ctccaatttt taacaatcgg cgactcttca
     2281 ggtcttacta actatcaatt tcttatacta ataacctacc gaatttccag aattccaata
     2341 cttccatctc cgaagtgtgt tatactattt acataagaca tcgcacctgg cttggtcagg
     2401 cagcatatat gtcttctttt ttttagccgt tgttttatat tttcaccccc aaaacaaacg
     2461 gcatacagtt ttgtaaaaaa acataggagg attttatgct taagtttgaa aacgtcagta
     2521 aagtgtatga agacgggttc aaggctgttg attctgtcag ctttgagatt ccggaaggcg
     2581 agctgctcgt tctcatcggc ccgagtggtt caggtaaatc gacaacgatg aaaatgatca
     2641 atcggatgca accgcataca agcgggaaaa tttcgataaa cggtaaggat aacaaatcgt
     2701 acgtcgcttc cgacctgcga agaggcatcg gctatgtgat ccaacagatc ggtttatttc
     2761 cccactatac gattgaaaaa aacattgcaa tcgtacccga gctgaacggc tggagccagg
     2821 ataaaatcaa acctcgggtc accgaactgc tggaattggt cggacttgat cccgaaattt
     2881 acgcaagtcg ctatccaaaa gaactttccg gcggacaaca gcagcgtgtc ggtatcgccc
     2941 gcgcacttgc gtctgatcct gaaatcattt taatggatga accgttcagc gcattggatc
     3001 cgattacaag ggaacagttg caaggcgaac tcatttcatt gcagaaaaaa ctgaataaga
     3061 ccatcatttt tgttacacat gacatggacg aggccctcaa aatgggtgac cgcatcgcaa
     3121 ttatgaaaga cggcaaactc cttcagttgg acactccaga aaaattactt catgaacctg
     3181 cccacggttt tgtcgaggag ttcattggca agcatcgaat cattcaaaac cctgaactga
     3241 tgcctgtcct tgacattatg tccgaatcgg tcgtcaccac tcttcctcaa agttctcctg
     3301 aaaaggcaat ttcacttatc cgccagagga aaattacaga tctcatcatt gtggatgaat
     3361 caaaagaatt gctaggcatc gtgtctgcct atgatgtcat caagaaagtg aacgacatcc
     3421 gcacaattgc agaaatcatg cagccgatcg gatacttctt ggaagacacc gcttctgcaa
     3481 aagacgccat catcatgatg gatgaaacgc cgttcggctt gattccgatc gtggatgaaa
     3541 agactagggt cgtcggtctt gttacacgcg gttcgttgct atccgcatta tccagccagt
     3601 ggacggaaac tgaaacggca gcggaggtgg ttgccatatg aataatttat cgatttggca
     3661 acaattgacc gaacaatcac agatgcgctg gagagaagtt cttgaagcga catccgtcca
     3721 tatccaactc gtgttcttct cgatgttgat cgccatcatt ctcggcgttt cactcggcat
     3781 ccttgtcacc cgggttccgc aattgacgac tctcgtactc ggcggtacgg gcgttatgca
     3841 gacgattcct agccttgcgt tgctcggctt catgattccc atcttcggga tcggtgtaaa
     3901 aacagcgatt gcagcgctat tcctttactc gctgctgccg attgcacgaa acacgtacgc
     3961 aggaatcacc gaagtcgacc cggctacgag ggaagccgct aagggaatgg gaatgacgag
     4021 cgcacaaatc cttctcaaag tggagctgcc actcgcgatt cccgtcatca tggccggaat
     4081 ccggacagct gccgtcatta acgtcggtaa cgctaccctc gccgcattca tcggcgcagg
     4141 cggccttggc gatttcattt tccttggaat cacgcgcggt atcgacggac taatcctgct
     4201 tggtgcaatc ccagcagccc tgcttgccat cattcttgaa gtgttcttca gctctgttga
     4261 gcgctggacg acgccgaaag gattgaagac ggaatgagga agaaattcat tggtttttta
     4321 ctcattgcag tcgtggcaag catgctttcg ggctgcattt tcattgaaaa agattcgctg
     4381 atcctcggtt cacggaacaa cacggagagc atcattttgt cccatgtgat gggacagttg
     4441 attgagaata aaacagacat caaggtcatt tataaagaaa accttggcgg ctcgaatgtc
     4501 gtatggaatg cgatgctgaa cggcaatatc gacgtcgttc ccgattatac ggggacgatt
     4561 gtcgtcaact attaccacga agagccgggg aacgccgagg agacattgga attgacgaga
     4621 cggcttgtcg gtgaagacgg catcatcgct ttcaacacat tcggcttcaa caacacgtac
     4681 accctcgcgt tggacgaatc aaaagcggaa gaactcggcg tcgtcacatt cagcgacttc
     4741 gctgcatatt cggaagactt cacgcttggc gcagtcttcg aattcattga ccgcccggac
     4801 ggactgcccg gcttccagaa ggaatacggg ttgaagttca agaacgtcaa aggcatggac
     4861 cacggcatca tgtaccgttc gatcagtgcc ggcgaagtcg acgtcatcaa ctcctacacg
     4921 acagacggtc aactgcagga gttcgacctg cgcgtgttgg aggacgacaa atcctacttc
     4981 cctccatacc acgcactccc gctcgtacgg aaagaaacgc tgaaagagta ccctgaaatc
     5041 gaagccgttt taaggcagct cgaaggcgcg atcgacgaag aggcgatgca aaaaatgaac
     5101 gccaaagtcg acaacgacgg catgatggtc gagcgtgttg cggaggaatt tttggtggag
     5161 agtgggttga ttgagggggt tgcgcggtag gagggtttat caccgcttaa cggctgttta
     5221 tcaccgctta gcgacacttt atcaccgctc agatcaagtt tatcaccgct tgggcacact
     5281 ttatcaccgt tcgaccatat ttatccatag aaaaggcagc ttaatgggct tcccaaggtc
     5341 atttagcccc gaatgatggt tatcaccgct caagcgaggt ttatcaccgt tcagtgggcg
     5401 tttatcaccg cttaacttca ctttatcacc gttcggaaca agtttatcac cgttcaacca
     5461 cttttctagt gttccaaggt accttacaac aaggaaccaa cgcaaaaagc gcacaaaatc
     5521 atgtgcgctt tttgtttttc ggaagaaatt gcatcgtgta tgtgcgattc cgatttgccc
     5581 cctcagtatg cagccccata ccttgtagga ttcgatgggc cgtttgttga tgttcaatgt
     5641 gtagaaatgc acgacaatct ttattgctca tctttcctcc aaatagcatg aaccaatcac
     5701 gaattgcctg cttatgggct tccctatctg caataccaca tgttgtacaa tgccagcttc
     5761 ttgaatgtct caccatacca acaaacccac aacccgggca aatcacaccc gtccgtatgt
     5821 cgctatgtcg aatggaatac gtttcacaaa tagaagaagg aatgaattca cgatgggagc
     5881 tgcctagctg catcatgagg tcgacgaacg tttcgtcatc caacaatggg gaggttgttg
     5941 ggagtttgcg gatgaaggag ggtaccgcac ttgggaataa aatagggata ttcgtatcga
     6001 aaacatcgat gcgttgcttg gcatacgcca acactacagc gccgtatacc ggcaatgcaa
     6061 tattccggct gtacagccag tcttgaaaca gttcgcaatt gctttccagt tgggcaatcg
     6121 ggcttttgaa cccgcttact tgtcctgaat ccagcacgcg gatcaattgg ggcggatttt
     6181 ccgtcaccgt cagttcgccc gcaatgtttt tcacttcgaa taaaaccgca taccatgggg
     6241 tgataaacag tgtatctact tgaaaatatg tactggaggc tagcgataga tcgtgaaaaa
     6301 tgccatgctt cattgaaaaa gcatagcttt caaacacctt gtccaactcc tgctctccgc
     6361 caaatccggc ttgtaccgcc gcatgcttcg agcgcaaagc aggaatcgct tcatggtggt
     6421 caggcaatcg cctcatcgcc gcctccagcc tctccaatac cagaggggcc tttcttagct
     6481 tatgaatgaa tctggttcaa ctccctacat tatattccgc tattcactag tttatcacgt
     6541 tcgactgatt attcaaccaa actccaaata ctcccacaca ttcccgaatg gatcgcggaa
     6601 acttatatac tttccgggcg ggcaatccgc gggttcagca ataatcaatt ccacgccatt
     6661 ctctctcaac ttgctcagag attgatggat gtcatccgtt ttcaacgtca gcacaattcc
     6721 tgaagaccct acattaaccc ccgcgttctc cgtctcctcc aacacgatcg gcaactcccc
     6781 gtggacaagc gttacgatct tcggtccata ctccttgttc acctcaaacc ccaatacccc
     6841 cgtgtaaaat tcaatcgcct cctgcatatc cggcacatga atgccaatta cacaaacttg
     6901 cgtcatacca attccccctg ccctttatat tgatcgatca ttctccgcaa aaatcgcatc
     6961 tgttgacttc cttcactcca ctgcatccgg atccactcgt actcccgctc cagtaaacgt
     7021 ccaatccgcc ccaccgcctg cacatgctct ccaggtgtta caaactcaag aatttccgtc
     7081 aactccaaaa caacggactc cggcaacgac cgttcaatcg tcttcaaccc gagcttcgcg
     7141 cgttccggtg cataccgata gagcagcact ctccctaaat gatgcacgac attcgtcagc
     7201 atcttaaccg cccaaatgtc atttcctcgt gcagccgtct tcgagtattg gaataaaaac
     7261 caccccacgt ccatgacatc atcccgatat tcttcctcct tcagctccag ccccagcgtt
     7321 tccataaatt catccattaa ccccttcgga tcatacagca ccttgaaatg gtcgccgccg
     7381 acgaatgatt ccttcgtcac tgtgaataaa tcgatatgca acagatcatc aaacaccgca
     7441 ataatctgag gggcaacaat gaaaatatca tccagtagaa tgatgtcgcg atacgcctgc
     7501 aaatgcgcca cccgcgccgc aaggaaatcc ttcttgtcct cctcgttcac caagcaataa
     7561 agatcgacgt ctgaatgcgc atcatgctct cctcttccca tcgagccttt tagaaagatc
     7621 gcctgtacaa gcgggtcctt cttcaaactg gcagcaatct tttcaacagc caactcctgc
     7681 ggcaacatat ccatcccccc taacctatct ttaccaattt ctactccgac agcccgttct
     7741 cctttatttg gtcagataaa aaaggaaggg atgccccctc cttcaattaa ataaatccaa
     7801 ccatatcgga taatcgaatt ggccatcgga tagttgaaaa aacattccaa atgcacctaa
     7861 atgggtaatc ccatattcaa catgataaac tggcgtgtag gttgaaaaga gcaagaaatt
     7921 taccctttca atttccattt tcacggatgg tggcggcgga ccatattttc cttcactgaa
     7981 ttgcaaatac tccttctccg acgggttggt tacgagtgca atgaggagaa taaacaataa
     8041 gactccgccg atcatatagc gttttttcat taattgcccc cctcaatcaa ttataattta
     8101 attttctgga aattaaatcc tcatgaagcc ctattcacac ccctataaaa caaaaaaaga
     8161 gcagtccaca gaccactctt tcccttctta cttcgaatcc gcccgatata aaaacaatgc
     8221 catttcgcca cggatgattg ctacgtcagt tccaaactct cctcttccta tgaacgcatt
     8281 ttcatagtat ataacataca actaacatgc aatgtaatct tcaaccattc aaccgccatg
     8341 catgattcat ccgacaaagg gtattctaat ctgttaggag gatgatgaaa atgacgccat
     8401 tttgtccgga aagctggccg gaagatccga cgccggcgag ccaggtgaat tgccaggaat
     8461 gcgggcttta tcaacaagga acgcggatgg tgtggggtga agggaatccg aaggcaccga
     8521 tcatggtgat tctcgataac ccaggcgcac gtgaagatcg tgaaggaaat ccgatggttt
     8581 gcggaacgcg gcagacgttg cagcaagccg tttatgaagc cggtttggat aaggagcaaa
     8641 tctacgtaac gtacattctg aaaagaaggc ccattcggaa atacgataaa gaacagaccc
     8701 gtgaaatttg catgcaccgc catttggatg gacagctaaa gcagcaaaaa cccgacctca
     8761 ttctttgcat ggggaatgtc gcagtacaat ccttcttcga aaatgccgaa gtagacgtaa
     8821 agggtttgcg cggcagctgg cacaacatca agggctaccc tacgaccgtc gcctatcatc
     8881 cacttgccgt caggcgtcgt cctaatttat ataagttgtt cttggaagat atcatgttag
     8941 tgaaatcaaa aattgaagat gctcacgtaa aaaaaggaag ctatcatgcc gaccattgaa
     9001 catgagatct cgatcaatgc ccctatccaa atctgttttg acgtggcgag aactgtggaa
     9061 gtccacgaag gaaaaacgat gctgacgaaa caaataacca ttagcggagt gacgaaaggt
     9121 ttgatggaac atggcgattc cgtcacttgg caaacgactc atttgggaat taaacaaaaa
     9181 ttaacgtctc aaattattaa aatggtaaaa ccatatcact tcaccgacgc catggttcaa
     9241 ggagtctttc aatcttttac gcacacacac gagttcattg aaagctgtgc ggaaacgatt
     9301 atgaaggata cctttaccta tcaatcccct tttggaatta tagggaaagc agcagatcaa
     9361 ttgtttttag aaagatatat gaggaacttt atagccctcc atgctaaaaa ggtgaaaaag
     9421 gcagcggaa
//
LOCUS       DASULI010000086         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1831160,
            whole genome shotgun sequence.
ACCESSION   DASULI010000086 DASULI010000000
VERSION     DASULI010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831160"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1493
                     /locus_tag="VFX34_01960"
     CDS             87..1493
                     /locus_tag="VFX34_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX5563699.1"
                     /translation="MTTEQTTLQQDTGEEVVRDYSLDRVPREERKAGWLSITNITFGI
                     ATAIFYFQMGSVMALQFGAPNALISAGYAIIVAGLLGTVIVYLSAKSGMNVNLLSRGG
                     GFGYIGASLTSLIYASNFIMYCAFEGLILISAVHHFLPSLPKWVLILVFGLIVIPLNW
                     FGIQQLDKLQKWSLPIFVLFLITAIVVSFIKPAIYTGPVFSYMPEGVQLGGTALLFCI
                     GMHNGIMGLTALLASDYARFLRPKDIKVGSLAIGFIPQIFCYGVMGGLGIWFGVRYLE
                     SDPGVYIVLLLGIGGALFTMLTQLRINVTNIYSSSLSLSNFFENIFRFTPGRRFWVVV
                     SALTAIALMLGGIVDHLATVMTFQGVFLFAWASILVTDALVVKKWLKIGPNYYVATQD
                     YLYKWNPVGVVSLLVSSVIGTIAALGYMGLFLQSTAAFFAAVLAAILTVIIAIMTKGK
                     YYLQQEPMNIVEEDKLKR"
     gene            complement(1542..>2358)
                     /locus_tag="VFX34_01965"
     CDS             complement(1542..>2358)
                     /locus_tag="VFX34_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014970135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5563700.1"
                     /translation="LMLTALLMVLLAGCGKDKAADTPAKEQKKEETVTLKVASLIPPM
                     TEILELVKPRLAEEGIDLEVVVLGDNVQPNSALAAKEVDANFFQHVPYMEEFNRNNNA
                     NLVPITPIYFANYGVYSKDFATVDELPEGSVIAMANDVSNIDRSLSLLAQHGVITLGE
                     KKDTYYTQADITDNPKNYKFEEVDLLMLARMYDDADAVVMTPAYAAPLGLTPKSDALL
                     TEGVENDFAITLVAREDNKDWEPIQKLADAMTSPEVRKFLEENYDETAIPAFE"
BASE COUNT          546 a          540 c          537 g          735 t
ORIGIN      
        1 gaggtgagca tttttaaaag aagtttgatt aaaaatttgc ctatgggctg aaggagtgag
       61 cgattcacaa taaggagctg gaatttatga caacagaaca aactactttg caacaagaca
      121 caggtgaaga ggtcgtccgg gattattcct tggaccgtgt acctcgtgag gaacgaaaag
      181 ctggctggct cagcataacg aacattacgt ttggcattgc tacagcaatc ttttacttcc
      241 agatgggaag tgtgatggct ctccaattcg gggctcccaa cgccctcatt tccgctggct
      301 acgcaatcat cgtagcaggc ttactcggta cagtcatcgt ttatttatcg gccaagtcgg
      361 gaatgaacgt gaacctatta tcccggggcg gcggcttcgg atatatcggg gcatcattga
      421 cttctctcat ttacgcatca aattttatca tgtattgtgc ctttgaaggc ctgattctta
      481 tatcagccgt acatcatttt ctaccttccc ttccaaaatg ggttttaatc cttgtattcg
      541 gactcattgt cattcctctt aattggtttg gaattcaaca actggacaag ctgcaaaaat
      601 ggtcattacc gatttttgtc cttttcctga ttacagcaat cgttgtttca ttcatcaaac
      661 ctgctattta tacgggtcct gtcttctctt atatgccgga aggagtacag cttggcggaa
      721 ccgcgctgct gttctgtatc ggtatgcata atgggattat gggcttaacc gctcttctcg
      781 cttctgatta tgcccgtttc ttacgcccta aagatattaa agtcgggtct ttagcaatcg
      841 ggttcattcc tcaaattttt tgttatggcg ttatgggagg attaggtatc tggttcggcg
      901 tccgttattt ggagtcggat ccaggcgttt acattgtgtt gctgctcgga attggcggcg
      961 cattatttac gatgttgacg caactgcgca ttaatgtcac aaatatttat agtagttcac
     1021 tctccttatc aaacttcttt gaaaatatct tccgtttcac ccccggccga cgtttttggg
     1081 tcgttgtctc cgcattgacg gcaattgcgt taatgcttgg cggaattgta gaccatcttg
     1141 caacggtcat gacattccag ggcgttttcc tatttgcatg ggcgtccatc ttggtgacgg
     1201 atgcgctcgt cgtcaaaaaa tggttgaaaa tcggaccgaa ttattatgtc gcgacacagg
     1261 attatttgta taaatggaat cctgtcggcg ttgtttccct cctagtttca agtgtcatcg
     1321 gtacaatcgc tgcgcttggc tatatggggt tattcctcca atcgacagct gctttctttg
     1381 ccgcagtgct cgccgcaatt ttgacggtca ttattgcgat aatgacaaaa ggaaagtatt
     1441 atttgcaaca agaaccaatg aatattgtcg aagaggacaa attgaaacga tgaacgataa
     1501 aagccatgcc cgattgagtt gggcatggct tttatcatct tttattcaaa cgcaggaatt
     1561 gcagtttcgt catagttttc ttctaaaaac ttgcgtactt ccgggcttgt cattgcatcc
     1621 gccaactttt gaatcggctc ccagtcttta ttgtcttcac gtgccactaa tgtgatcgcg
     1681 aagtcatttt cgacgccttc cgtaagcagt gcatcgcttt tcggcgttaa accgagtggc
     1741 gcggcatatg ccggtgtcat gacgaccgca tccgcgtcat cgtacatgcg cgcaagcatg
     1801 aggagatcga cttcttcgaa cttgtagttt ttcgggttat cggtaatgtc cgcttgcgtg
     1861 taatatgtgt ctttcttttc gcctaacgta atgacgccat gctgtgcaag cagcgacaac
     1921 gaacggtcga tgttggaaac gtcgttcgcc attgcgatga cggatccttc aggcagttca
     1981 tccactgtcg cgaagtcttt tgaataaacc ccgtaattcg caaaatagat cggtgtaatc
     2041 ggaactaggt ttgcgttatt attccggttg aattcctcca tgtacggtac gtgctggaag
     2101 aagttcgcgt ccacttcttt cgctgcaagc gcactgttcg gctggacatt gtcgcctaat
     2161 acgacaactt ccagatcgat tccttcctca gcaagcctcg gtttcacaag ctcaaggatt
     2221 tccgtcatcg gtggaatcaa tgaagcaact ttcaatgtca ctgtttcttc tttcttctgt
     2281 tccttagcag gggtgtccgc tgctttatcc ttgccgcagc ccgctaataa gaccattagc
     2341 aatgccgtta gcataagt
//
LOCUS       DASULI010000087         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1113909,
            whole genome shotgun sequence.
ACCESSION   DASULI010000087 DASULI010000000
VERSION     DASULI010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113909"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFX34_01970"
     CDS             complement(<1..967)
                     /locus_tag="VFX34_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX5563701.1"
                     /translation="MIELTGSSLTLEQMEGILYKGQQVDLNAEALERVQKSREAVDRI
                     VENDKTVYGINTGFGKFSDVKIDEKDTKALQLHLIRSHACGVGEPFSETVSRAMVVLR
                     LNALMKGFSGIRVEVLERFAFMVNNGIHPVIPQQGSLGASGDLAPLSHLALVLLGEGF
                     VWRDGGHVPAAQVWKEFGMEPIVLEAKEGLALINGTQAMTAQGVVNYLEAEKLAYSSE
                     WIAAMTMEALHGITDAFHPAIHEARGYKEQVDVASRMLNWLEDSELTTVQGEKRVQDA
                     YSIRCIPQVHGASWQVLSYVKEKLEIEMNAATDNPLIFEDGDVVVS"
     gene            complement(1139..>2144)
                     /locus_tag="VFX34_01975"
     CDS             complement(1139..>2144)
                     /locus_tag="VFX34_01975"
                     /inference="COORDINATES: protein motif:HMM:NF037393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5643 domain-containing protein"
                     /protein_id="HEX5563702.1"
                     /translation="IIGTTGETEDVKVTLEGIITDETSLIVFSKIENNKKHPGRYLSG
                     YRILDTDGKEIPNDQSFATSWNQENDLLATNMTEIKFKGPVPTEEMILEYRFADDIGN
                     DQVIETIRIPFKVELKPVKREQYIVNKTVVVDGQKIHIHSITIGPIKTAVEVEYDAEN
                     TKKIFGFEDLRIVDEKGEVWSSIKNGSSASWSEDSPVISYFLQSNYFRDTDHLYLQFN
                     KLMAMDKDEAELIIDTEKQVIVKQPKDNRFGNLMIYGPYMEIELRGGKDYHHNPFSKI
                     YDADGKELHKKSSSFSKNDEEIVTLGIELPNENYKNPIRFPLYGYPSYIKGDVKIKIK
                     "
BASE COUNT          543 a          531 c          395 g          675 t
ORIGIN      
        1 cggatacgac gacatcccca tcttcaaaaa taagcgggtt atccgtggca gcattcattt
       61 caatctccag tttttccttc acataagaaa gcacttgcca gctcgctcca tggacttgcg
      121 gaatgcagcg gatcgaatag gcgtcttgta cacgcttctc cccctgcacc gtcgtcaatt
      181 cactgtcctc cagccaattc aacatcctcg aagcgacatc cacttgctct ttataacctc
      241 gcgcctcgtg gatagcagga tggaaggcat ccgtaattcc atgcaacgcc tccattgtca
      301 tagcggcgat ccactcactg ctatatgcca gcttctccgc ctccaaataa ttgacgacac
      361 cttgtgcagt cattgcctgc gtgccgttaa tcaatgctag cccctctttt gcttccagaa
      421 caatcggctc cattccgaat tccttccaaa cttgggcagc aggcacatgc ccgccatctc
      481 gccaaacgaa cccttcccct aacagaacga gcgccaaatg ggaaagtggc gcaagatcac
      541 ctgatgcccc gagcgaccct tgctgcggga tgaccgggtg aattccgtta ttcaccatga
      601 atgcaaaccg ttccagcact tcaacgcgga ttccggagaa tcccttcata agtgcattta
      661 atcgcagtac gaccatagct cgcgaaacag tttcggaaaa aggttcacca acgccgcatg
      721 cgtgtgagcg gatcaaatgc aattgcaatg ccttcgtatc tttttcatca attttcacat
      781 cgctgaattt accgaagccg gtattgatgc catacacggt cttgtcattt tcaacaatcc
      841 gatcaaccgc ttccctgctc ttttgcacac gctccaaagc ctcggcattc aaatcgactt
      901 gctgcccttt atataaaatg ccttccatct gttcgagtgt aagagaagat cctgtcaatt
      961 caatcatcca aaccatcctt tcagcaagag aaaaaattgt tatcctactg tccatcgtac
     1021 acgatgccaa aagggttttg gtgattttta cgaaaatttg ttctatagta atgtgatgtt
     1081 gttttagcgt gatgatgcga tgaaaaaacg acggatatca gttatccgtg gatcctagct
     1141 acttgatttt gatttttacg tcgcctttaa tataggaagg atacccgtac agcgggaatc
     1201 ttatcggatt tttatagttt tcatttggca attcaatgcc gagcgtaaca atttcttcgt
     1261 cattttttga aaatgaactc gattttttat gaagctcttt gccgtcagcg tcataaattt
     1321 ttgaaaacgg gttatgatga tagtcttttc cgcctcttag ttcgatttcc atatatggac
     1381 cataaatcat taagttgcca aatcgattat cctttggctg cttaacgatt acttgctttt
     1441 ctgtgtcgat gatcaattca gcttcgtctt tatccatcgc catcagcttg ttgaactgga
     1501 ggtaaagatg atccgtatct ctgaaataat tactttgcag aaagtaacta atgacaggcg
     1561 aatcttcact ccagcttgct gatgatccgt ttttgatgga actccatact tcaccttttt
     1621 catctacaat gcgtaagtcc tcgaatccga agattttctt cgtattttcg gcgtcatact
     1681 ctacttctac agctgttttt attggcccga tcgtgattga gtggatatgg attttctgtc
     1741 catcgacgac aaccgttttg ttaactatat attgttctct tttcaccggt ttcaattcta
     1801 ctttaaaagg gattcgaatt gtctcaatta cttgatcatt tccaatgtca tcagcaaatc
     1861 gatactccaa aatcatttct tcagtaggaa caggaccctt aaattttatc tctgtcatat
     1921 tggtcgctaa caaatcattt tcttgattcc acgacgtagc aaaggattga tcatttggaa
     1981 tttccttccc gtctgtatcg agtattcgat atcctgataa ataccgtcca gggtgctttt
     2041 tattattctc gatttttgaa aagacgatta aactcgtttc atcggtaata atgccttcga
     2101 gcgtgacttt tacgtcctct gtttcacctg ttgtcccgat aatc
//
LOCUS       DASULI010000088         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_996823, whole
            genome shotgun sequence.
ACCESSION   DASULI010000088 DASULI010000000
VERSION     DASULI010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996823"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFX34_01980"
     CDS             complement(<1..742)
                     /locus_tag="VFX34_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563703.1"
                     /translation="MEFDDKDFEVVGNQSESQEVITRPQMSMFKESWIRLKKNKGAML
                     SLVVLSILIVMAIIGPHLNEYSYEQTDYDKVFQKPSAEHWLGTDRFGRDQWTRIWEGT
                     RISLYIAFLAATLDLIIGVLYGAVSALLGGRVDNVMQRIIEVLVGIPNLILIILLIML
                     MKPGIVTITIAMVITGWVNMARLVRSQVFKLKTQEFILASRALGASNGRLIKEHFIPN
                     TLGLIIVNMMFTIPAAIFTEAFLSFIGLG"
     gene            complement(799..1734)
                     /locus_tag="VFX34_01985"
     CDS             complement(799..1734)
                     /locus_tag="VFX34_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563704.1"
                     /translation="MKKFIVVRVVNIILTLVVIATATFFLMKLLPGSPFDEEKLAGLT
                     EEARLEFYAKYGLDKPLIMQYGIYMENLVKGDLGTSFYFKGQKVESLIKDRIGPSALL
                     GLQAVILGMSIGLILGMVAAFRHNTAWDYFTMFIAVIGVSIPNFVFAALLQYYIGLKW
                     GILPVAFWESYSSSVLPTIALAMMVIALIARFTRNEMLEVLQQDYILTAKSKGLSRWS
                     VIFNHTLRNSLIPIITILGPITVSLLTGSLVIENIFGIPGIGSLFVDSIKVNDYTTIM
                     GLTLFYSAFYILTILVVDLLYGIIDPRIRLTESKG"
     gene            complement(1778..>2615)
                     /locus_tag="VFX34_01990"
     CDS             complement(1778..>2615)
                     /locus_tag="VFX34_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX5563705.1"
                     /translation="TGAADQYQFDDGDLALACASHNGERQHTERAASILARLDLEVAN
                     LKCGTHPPRRQEAYEELIKSGGEVTPIHNNCSGKHSGMLATAKHMNETVDDYYKIEHP
                     VQQRILSVISELTDVPVDDIQIGIDGCGVPVHGIPLRNLALGFARMADPSGLEETRQQ
                     SIRRVTDAMMNAPEMVGGTSRFCTDFMKHMDGKMFGKVGAEGVYCVGVPKAGIGIAVK
                     IEDGNSRATNAVIMEILSQMDLIDEKEAEALESYHFPTLLNARKEKVGMLKPVFSIYQ
                     KV"
BASE COUNT          674 a          712 c          546 g          683 t
ORIGIN      
        1 accctaagcc gatgaaactt agaaatgcct ccgtaaatat tgccgcagga atcgtgaaca
       61 tcatatttac gatgatcagg ccaagcgtat tggggatgaa gtgctctttg atgagcctgc
      121 cattcgaagc gcccaatgcc cttgaagcga ggatgaattc ctgggtcttc agtttaaaca
      181 cttgtgaacg gacgagcctt gccatattga cccatcctgt tatgaccatc gcgatggtaa
      241 tcgtaacgat gcccggtttc atcaacatga tcagcaagat gatcaggatg agatttggaa
      301 tgccgaccaa tacttcaatg atccgctgca tcacattatc caccctgccg cctaacagag
      361 cggaaaccgc accatacaaa acaccgataa tcaagtcgag agtcgcagct aaaaacgcga
      421 tatataatga aattcttgtt ccttcccaaa tccgcgtcca ttggtctcgg ccgaaacggt
      481 ccgtccctag ccaatgttct gcactcggtt tttggaacac tttatcgtaa tctgtttgct
      541 cgtaggaata ctcattcaaa tgcggcccga tgatcgccat gacaatcaaa atcgataaga
      601 cgaccaagct cagcatcgcc cctttattct tcttgaggcg gatccagctt tccttgaaca
      661 tgctcatttg cggccgggtg atgacttcct gtgattccga ttgattgccg acgacttcaa
      721 agtccttgtc atcgaactcc ataccggcca attcgtccag tccgccctca tattctttaa
      781 cgttgtttgc catcatcctc accccttcga ctctgtcaaa cgtattcgcg gatcaatgat
      841 tccatataat aaatcgacga ctaaaatggt caggatataa aacgcactgt aaaacagcgt
      901 caatcccatg atagtcgtgt aatcattcac tttgatcgag tcaacgaaca agcttccgat
      961 ccccggaatg ccgaaaatat tttcgataac gagagaaccc gtgagcaagc tgacggtgat
     1021 cggccccaat atcgtaatga ttggtatcag tgaattcctg agcgtatggt tgaagatgac
     1081 actccacctt gaaagccctt ttgattttgc ggttaaaata taatcctgct gcagcacttc
     1141 tagcatttca ttccgcgtga accgtgcgat gagggcaatg accatcatcg ccagggcgat
     1201 tgtgggcaac accgaactgc tatagctctc ccagaaagcg acgggaagga ttccccattt
     1261 caatccgata taatattgca gcaacgccgc aaaaacaaag ttcggtatgg aaacgccgat
     1321 gacggcaatg aacatcgtga agtaatccca agcggtatta tgcctgaacg cagcgaccat
     1381 acccagaatg aggccgatgg acattcctaa aatgaccgcc tgcaagccaa gcaatgcgga
     1441 aggcccgatc ctatctttga tgagcgattc aaccttttgc cccttgaagt aaaaggacgt
     1501 tcccaagtcg cctttcacca agttttccat atagattccg tattgcatga tcagcggttt
     1561 atccaatccg tatttggcat agaactcaag cctcgcctct tccgtcagac ccgccagctt
     1621 ctcttcatca aagggtgatc ccggcaataa tttcatcaag aaaaatgtgg cggtcgcaat
     1681 cacgactaat gtcaagatga tattaacgac tctgacgaca atgaatttct tcatcccttt
     1741 caccccttca catgaatatc ctattcataa gttcgtctta cactttttgg taaatggaaa
     1801 acacgggctt cagcatgccg accttctctt tccttgcatt caacagagta gggaaatgat
     1861 aagactcaag tgcctcggct tccttttcat caatcagatc catctggctg aggatttcca
     1921 taatgacggc gtttgtcgca cgactattgc catcctcaat cttgacggcg atgccgatgc
     1981 ccgctttcgg cacgcctaca caataaaccc cttccgcacc gactttgccg aacatcttcc
     2041 cgtccatatg cttcatgaaa tccgtgcaga aacgggaggt gccgccaacc atttcgggcg
     2101 cattcatcat ggcgtccgtc acgcggcgga tcgattgctg ccgtgtttct tccagcccgc
     2161 ttggatcagc catccttgca aaaccgagcg ctaagttccg caatgggatc ccatggaccg
     2221 ggacgccgca cccgtcgatg ccgatctgga tatcgtccac tggcacatcc gtcaactcgc
     2281 taatgacaga taggattctc tgttgcacgg ggtgttcgat tttataataa tcgtccactg
     2341 tttcattcat atgtttcgct gtcgccaaca ttccgctatg ttttcccgaa cagttattat
     2401 ggatcggcgt tacctcaccg ccgcttttga tcagttcttc ataagcctcc tgccttcttg
     2461 gaggatgtgt accgcacttt aaattggcca cttccaaatc caatcgggct aaaatggatg
     2521 cggctctttc cgtatgctgc ctttcgccgt tatgcgaagc gcaagccaat gccaaatccc
     2581 catcatcaaa ttgatattga tcggccgcgc ccgtt
//
LOCUS       DASULI010000089         6741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_746849, whole
            genome shotgun sequence.
ACCESSION   DASULI010000089 DASULI010000000
VERSION     DASULI010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746849"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /gene="speD"
                     /locus_tag="VFX34_01995"
     CDS             <1..693
                     /gene="speD"
                     /locus_tag="VFX34_01995"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX5563706.1"
                     /translation="DEQYNSERLTNILYDVTEIVGAHVLNVSKQDYDPQGASVTILIT
                     EETLPVAVIDESCNRGEIDILKTRDSVVGHLDKSHVTVHTYPEYHPDNSIATFRVDIE
                     VSTCGEISPLNALDYLIGSFDSDIITTDYRVRGFTRDDKGKKLFMDHKMTSIQDYIDS
                     ETLQKYDAIDVNVYQSNIFHTKLLIKDINLQNYLFNTDVYEIPPKERLNITNNLRKEM
                     IEIFSGSNIYEE"
     gene            737..2164
                     /locus_tag="VFX34_02000"
     CDS             737..2164
                     /locus_tag="VFX34_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5563707.1"
                     /translation="MEALNNYKKMRVVPFDVPGHKRGRGNEELTSFLGENCMSVDVNS
                     MKPLDNLIHPVSVIREAEELAAEAFGAKHAFFMVNGTTSAVQAMVMTACKAGEKIIMP
                     RNVHRSAINALILSGAIPVYVNPGVNNELGIPLGMSVKEVEKTILEHPDAKAILINNP
                     TYYGICSNMQAITDLAHRHGMLVLVDEAHGTHFYFSDDLPASAMSVGADMASVSMHKS
                     GGSLTQSSLLLINNEVSEGYTRQIINLTQTTSGSYLLMSSLDISRKNLALNGKEIFRK
                     VGEMAQYTRDEINKIGGYYAFSDELKNGDTIFDFDTTKLSVHTLEIGLAGVEVYDILR
                     DEYDIQIEFGDIGNILAYISVGDRRLDLERLVSALAEIKRRYSKDKSGLFDHEYITPQ
                     VIYTPQKAFYAPKETLPINESAGRIASEFVMAYPPGIPIVAPGERITPEILEYIQYCK
                     DKGSFMTGTEDSKIEYINVLKEAEQ"
     gene            2161..3012
                     /gene="speE"
                     /locus_tag="VFX34_02005"
     CDS             2161..3012
                     /gene="speE"
                     /locus_tag="VFX34_02005"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX5563708.1"
                     /translation="MNLWFVENHSPNVQFSMRVIEHLYTGKSEFQKIDVLQTAEFGRV
                     LTLDGYVMVTEKDEFIYHDMITHVPMATNPGIKKVLVIGAGDGGTIRELTKYDSIEHI
                     DMVEIDEMVVDVCREFLPQTASKLDDPRVHLHFEDGLKFVRSRNDEYDLIIVDSTDPF
                     GPGEGLFTREFYGNCYKALNEDGILVNQHESPFYEEDALGMQRAHKRIIGFFPVCEVY
                     QLHIPTYPSGHWLFGFASKKYDPIKDLDADAWNQLGLQTKYYNTDIHVGSFALPNYVK
                     EQLKDVE"
     gene            3002..3853
                     /gene="speB"
                     /locus_tag="VFX34_02010"
     CDS             3002..3853
                     /gene="speB"
                     /locus_tag="VFX34_02010"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010650547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX5563709.1"
                     /translation="MLNKNIETFIGCDNEYDESGIVVFGAPFDSTTSFRPGTRFASKV
                     MRGESFGIETYSPYQDKDLEDINVYDGGDLELSFGNTERALGQIEEYTRKVVQDGKIP
                     CMIGGEHLVTLGSVRAVAEQFPDLHIIQFDAHADLRDDYLDEKLSHATVIHRVWDIVG
                     DDRIFQFGIRSGDRSEFQWGKDRVFTNKFNFNGLEDIVERLQGKPVYLTIDLDVLDPS
                     VFPGTGTPEAGGVSFMELLQAILTVSGLNIVACDVNELSPIYDQSGVSTAVACKVLRE
                     LILAIHS"
     gene            3878..5077
                     /locus_tag="VFX34_02015"
     CDS             3878..5077
                     /locus_tag="VFX34_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEX5563710.1"
                     /translation="MGKALIIGAGGVASVVVHKCVQVPDVFEEICIASRTKSKCDALK
                     EKLDGGRTKIQTAQVDADNVEELVELINSFKPDIVINVALPYQDLTIMDACLETGVHY
                     LDTANYEPLDTAKFEYKWQWAYREKFEEAGLTAILGCGFDPGVTGVFSAYAQKHYFDE
                     IHQIDIVDANAGDHGYPFATNFNPEINIREITANGRYWENGEFVETPPLSEKRVYDLP
                     EIGPKDVYLLYHEELESLAENIKGIKKIRFWMTFSEKYLTHLKVLENVGMTSIEPINF
                     EGQEIVPLQFLKAVLPDPASLGPRTKGKTNIGCIFQGVKDGEEKTYYVYNVSDHQECY
                     REVGSQAISYTTGVPAMIGAMLFLKGEWKKPGVYNVEEFNPDPFMEALNEYGLPWQED
                     FNPELID"
     gene            5095..6237
                     /gene="nspC"
                     /locus_tag="VFX34_02020"
     CDS             5095..6237
                     /gene="nspC"
                     /locus_tag="VFX34_02020"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEX5563711.1"
                     /translation="MKLNFDPYAVPSPSYVVDEGLLIKNLELLKSVIDRTGCKILLAQ
                     KGFSMFSVYPLIGQYLDGVTSSGLLEAKLGYEEMGKEVHTYSPAFSEADFEEILSYSN
                     HLVFNSFDQWRRFRDKVKNYPKPIECGIRINPEYSEIEVDMYNPCFTFSRFGVTLENF
                     EPEELEGLSGLHFHTMCEQNSDTLARTIQVVDEKFGKYLKGMKWINFGGGHHITRPDY
                     DIETLIESILFMKEKYGLDVYLEPGEAIALNTGFLVTTVLDTMHNGMPLAIVDTSASC
                     HMPDVLEMPYRPEIVDAGMPNEKAITYRLGGPTCLAGDVIGDYSFDHPLTAGDRLVFC
                     DMAHYSMVKNNTFNGMELPAIVLNTVADGPKVIKEFGYEDFKERLS"
     gene            complement(6491..>6741)
                     /locus_tag="VFX34_02025"
     CDS             complement(6491..>6741)
                     /locus_tag="VFX34_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563712.1"
                     /translation="DLLKGKDLEIIAASLLVLGKLKVDSVILYRNSPTVTVNLLGAFQ
                     EESKNGNKMADFLNENGDITIDEIFEGIQSRMNKGQGR"
BASE COUNT         1881 a         1461 c         1647 g         1752 t
ORIGIN      
        1 gatgagcagt acaattcgga acgattgacg aacattctat atgatgtgac ggagatcgtc
       61 ggagcccatg ttttgaacgt gagcaaacag gattatgatc cgcaaggtgc aagtgtcacc
      121 attcttatta cagaagagac actacctgtc gcagtgatcg atgaatcttg caatcgcggg
      181 gaaatcgata ttttgaagac ccgggattcg gttgttggcc atttggataa aagccatgtc
      241 accgtccata cgtaccctga atatcacccg gataattcaa tcgccacttt ccgtgtcgat
      301 atcgaagtgt cgacttgtgg tgaaatttcc cctttgaacg cattggatta tctcatcggc
      361 agcttcgact cggacattat cacgacggac tatcgcgtac gcggttttac tcgtgatgat
      421 aaagggaaga agttattcat ggaccataaa atgacgtcca tccaagatta tatcgacagt
      481 gagacgttgc agaaatacga tgccatcgat gtcaatgtat atcaatcaaa tatttttcat
      541 acaaaactgc tgatcaaaga catcaattta caaaattacc ttttcaacac ggatgtgtat
      601 gagatacctc cgaaagaaag gctcaatatt acaaacaact tgcgtaaaga gatgattgaa
      661 attttcagcg gttccaatat atacgaagag tgagaggacg gtgttggaca ttctttcaca
      721 acagaaagcc ccgatcatgg aggctctaaa caattataaa aagatgcggg tcgttccttt
      781 tgatgttccc ggccataaac gtggcagagg aaatgaggag ctgacctctt ttttaggtga
      841 gaattgcatg tcggtcgatg tcaattcgat gaagccatta gataatctga tccaccccgt
      901 ttcagtcata agggaagccg aggagcttgc agctgaagca ttcggagcga agcatgcctt
      961 tttcatggtg aacgggacga cttccgctgt acaagcgatg gtgatgacgg catgcaaggc
     1021 gggcgaaaag atcatcatgc cccgcaatgt gcaccgcagt gccatcaatg cactgattct
     1081 gagcggagct attcctgtct atgtcaatcc aggcgtgaat aatgaactcg gaattccttt
     1141 aggcatgtcc gtgaaggaag tggaaaaaac gattcttgag catcccgatg cgaaagcgat
     1201 tttgatcaat aacccgacct attatggaat ctgttcaaat atgcaggcga tcacggatct
     1261 tgcccatcgc cacggaatgc tcgtccttgt agacgaagcg catggtactc acttttattt
     1321 cagtgacgac ttgcctgcct ccgcaatgtc ggtcggtgcc gacatggctt cagtgagcat
     1381 gcacaaatcc ggtggttcat tgacccaaag ctcattgctt ttgatcaata atgaggtgag
     1441 cgaaggctat acgaggcaaa tcatcaattt gacgcaaacg acgagcggct cgtatttgct
     1501 catgtcgtcc ctcgatattt cacggaaaaa ccttgcgttg aatggcaaag agattttccg
     1561 gaaagtcggt gaaatggcac agtatacccg tgatgaaatc aacaagatcg gcggttacta
     1621 tgctttttcg gatgaactga aaaatgggga cacgattttt gattttgata cgacgaagct
     1681 ttccgtccat acacttgaga ttggactggc gggcgtcgaa gtgtatgaca ttttgcggga
     1741 tgaatatgac attcaaatcg agttcggaga catcggcaat attctggcat acatatccgt
     1801 cggagaccgc cgtctggatc tcgaacgcct cgtttcggca ctagctgaaa tcaagaggcg
     1861 ctacagcaag gacaaaagcg gcttgtttga tcatgaatat attacgcctc aggtcatcta
     1921 tacgcctcaa aaagcgttct atgcaccaaa ggagacgctt ccgatcaacg aaagcgccgg
     1981 ccgtattgcc agcgagttcg tgatggccta tccgcctggc ataccgattg ttgcgccagg
     2041 tgaacggatc actcccgaga ttttggaata tatccaatac tgtaaggaca aagggagctt
     2101 catgacgggt acagaagata gcaaaatcga atatatcaat gtgttgaagg aggccgagca
     2161 atgaacttat ggtttgtgga aaaccactct ccgaatgtcc aattttcgat gagggtcatt
     2221 gaacatctct acacgggaaa aagcgaattc cagaaaatcg atgtgctcca aaccgctgaa
     2281 ttcgggcgtg tcctcacatt ggacggatat gtcatggtca cggaaaaaga tgaattcatt
     2341 tatcacgata tgattaccca tgttcctatg gcgaccaacc ccggcattaa gaaagttctg
     2401 gtcattggtg ccggagatgg cgggacgatc cgtgaattga cgaaatacga ttcaatcgag
     2461 catattgaca tggttgaaat cgatgaaatg gttgtcgatg tatgcaggga gttccttccc
     2521 caaaccgcaa gcaagctcga tgatccacga gtccatctcc atttcgagga cgggctgaaa
     2581 tttgtccgtt cacgcaatga tgaatacgac ctgatcatcg tcgattcgac ggacccattc
     2641 ggacctggag aaggcttgtt tactagggag ttttacggca actgctataa ggcgctcaat
     2701 gaggatggca tccttgtcaa tcaacacgag agccctttct atgaagaaga cgcactcggg
     2761 atgcagcggg ctcataaacg gatcatcggt ttcttcccgg tatgcgaagt gtaccaattg
     2821 catatcccga cgtatccatc gggccattgg ctgttcggtt ttgcatcgaa aaaatacgat
     2881 ccgatcaagg atctggatgc agatgcttgg aatcagctcg gattgcaaac gaagtattat
     2941 aacaccgaca tccatgtcgg ctcatttgca ttaccgaact acgtaaagga gcaattgaaa
     3001 gatgttgaat aaaaatatcg aaacgttcat cggatgtgac aatgaatatg acgaatcagg
     3061 catcgtcgtt ttcggggccc ctttcgattc gacgacgtca ttccgtcctg gtacacggtt
     3121 cgcgagcaag gtgatgcgcg gtgaatcatt cggaatagaa acatacagtc cttatcagga
     3181 taaagatctg gaagacatca acgtctatga tggcggagat cttgagttaa gtttcggaaa
     3241 tacagaacgg gctttaggac aaatcgagga atacacgcga aaagtcgtgc aagacggcaa
     3301 aatcccttgc atgatcggcg gtgagcattt agtaacgctc ggatcggtcc gagctgtggc
     3361 ggagcaattc cctgatttgc atatcatcca attcgatgcg catgcggatt tgcgtgacga
     3421 ttacctagat gagaaattat cacatgcaac agtcatccat cgtgtatggg acattgtcgg
     3481 ggatgaccgg attttccaat tcggcatccg ttccggtgac cgcagcgagt tccaatgggg
     3541 caaggatcgt gtcttcacga ataaattcaa tttcaatggt ctggaagaca tcgttgaaag
     3601 attgcaaggg aaaccggtgt atttgacgat cgatctggat gtattggacc cttctgtctt
     3661 tcctggtacg gggacgcctg aagctggcgg cgtcagtttc atggaattat tgcaagcgat
     3721 tttgacagtc agcggcctga atattgtcgc ctgcgatgtc aacgaactgt cgccgatcta
     3781 tgatcaaagc ggtgtttcga cagcggtcgc ttgtaaagtg ctgcgtgaac taattttagc
     3841 aattcattca taaaaataat attgggtggt gttttgattg ggtaaggcgt taatcattgg
     3901 agcaggtgga gtagcaagtg ttgttgtgca taaatgtgtg caagttcctg atgtatttga
     3961 agaaatttgc atcgcgagcc gtacaaaatc gaagtgtgat gcgttgaagg aaaagcttga
     4021 cggcggccgc acgaaaatcc agacggcaca agtcgacgca gacaatgtag aagaattggt
     4081 tgaactgatc aacagcttca agccggacat cgtcatcaac gtcgcattgc cataccaaga
     4141 tttgacgatc atggatgcat gccttgagac tggcgtccac tatttggata cagcaaacta
     4201 tgagccgctc gatacggcta aattcgaata caaatggcaa tgggcatacc gcgaaaagtt
     4261 tgaggaagca ggcttgactg caatcctcgg ctgcggcttc gacccgggcg taaccggcgt
     4321 cttctccgct tatgcacaaa agcattattt tgacgaaatc catcagattg atattgtgga
     4381 tgcgaatgcc ggtgaccacg gttatccatt cgccacgaac ttcaacccgg agatcaatat
     4441 ccgtgaaatt acggcaaacg gccgttactg ggaaaacggc gagttcgtcg aaacaccgcc
     4501 actttccgaa aaacgggtat acgacctgcc ggaaatcggt cctaaagatg tttaccttct
     4561 ctaccatgaa gaattggagt cacttgcgga aaatattaaa ggcatcaaga aaatccgttt
     4621 ctggatgacg ttctcggaga aatacttgac gcatttgaaa gtgcttgaaa atgtcggcat
     4681 gacatcaatc gagccgatca atttcgaagg acaggaaatc gttccattgc aattcctgaa
     4741 ggcggttctt cctgaccctg cttccctcgg accacgcacg aaaggaaaaa cgaatatcgg
     4801 ttgtattttc caaggcgtga aagacggcga agagaagacg tattacgtct ataatgtatc
     4861 cgaccaccag gagtgttacc gcgaagtcgg ttcccaagca atttcgtata cgactggcgt
     4921 acctgcgatg atcggcgcga tgctcttcct gaaaggcgaa tggaaaaaac ctggcgttta
     4981 taacgtcgag gagttcaatc cggatccatt catggaagca ttgaacgaat atggtctgcc
     5041 ttggcaggaa gatttcaacc cagagctgat cgattgatag gaggcgtgca tcaaatgaaa
     5101 ttgaatttcg atccatatgc agtcccctcc ccttcgtatg ttgttgatga agggctgttg
     5161 attaaaaacc ttgaactgct gaagtcggtg atcgacagga caggctgtaa aattttgtta
     5221 gcccaaaaag ggttttcgat gttctccgtc tatccgctga tcggacaata tttggatggg
     5281 gtgacgtcaa gcggtttgtt ggaagcgaaa ttaggatacg aagagatggg gaaagaagtg
     5341 catacgtatt cccctgcttt ttcagaagct gattttgagg aaatcctatc gtactccaat
     5401 catcttgtgt tcaactcgtt tgatcaatgg agacgtttca gggataaagt gaaaaactac
     5461 ccgaaaccga ttgaatgcgg gattcggatc aatcctgagt actcggagat tgaagtcgat
     5521 atgtataacc cgtgcttcac attttcacgt ttcggcgtga cgcttgagaa tttcgaaccg
     5581 gaggagctgg aaggactcag cggcctgcat ttccatacga tgtgcgagca gaactccgac
     5641 acgcttgccc ggacgattca agttgtcgat gaaaaattcg gcaagtattt gaaaggcatg
     5701 aagtggatca atttcggcgg cggccatcat attacccgtc cagattacga tattgaaaca
     5761 cttatcgaat caattctttt catgaaagaa aagtatggac ttgatgtcta tttggaacct
     5821 ggtgaagcga ttgccttgaa cacgggattc ctcgtaacga cagtgctgga tacgatgcat
     5881 aacggaatgc cgcttgccat agtggataca tctgcttcgt gccatatgcc tgacgtactg
     5941 gaaatgccgt accgtcctga aatcgtggat gccgggatgc cgaatgagaa agcgatcacg
     6001 taccggttgg gcggacctac ttgcctcgca ggtgatgtca tcggcgatta ctcattcgac
     6061 catccattaa cggcaggtga caggcttgtc ttctgtgaca tggcccatta ttccatggtg
     6121 aagaacaaca cattcaacgg gatggagtta cctgcaattg tattgaatac tgttgctgat
     6181 ggtccgaaag tgatcaagga attcggctat gaggatttta aagagcgttt gtcgtaaaat
     6241 aaaagggttg cccatgactt gaagttaagt ctggcagccc ttttactata ctgatgatac
     6301 ccgttgtctt accattaagg tgcaccggta agtttgctaa tatcagtaga accaaagaag
     6361 actaataaca ataaaacaat tagaatgact acgtttatga atgaacttcc cgaaaaacct
     6421 gtcgtgttgc ccgtctcgtt tcctggcgtg ttgcctatcg tgctattgct cacgtaacgg
     6481 cgtaccattc ttatctcccc tgtcctttat tcatccgact ttgaatccct tcaaagattt
     6541 catctatagt aatatcccca ttttcattta aaaaatcagc cattttatta ccatttttcg
     6601 actcttcctg gaaagctccg agtaaattca ccgtaactgt cgggctattc cggtaaagga
     6661 tgacagaatc tacttttagc ttgcccaaaa ctagaagtga tgctgcaatg atttccaaat
     6721 ctttcccttt taataggtcg t
//
LOCUS       DASULI010000090         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1229564,
            whole genome shotgun sequence.
ACCESSION   DASULI010000090 DASULI010000000
VERSION     DASULI010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229564"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /locus_tag="VFX34_02030"
     CDS             complement(<1..2124)
                     /locus_tag="VFX34_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HEX5563713.1"
                     /translation="MSLRFITGRSGTGKTMFIQKEVAEELERDPGGRPIFVVVPDQMS
                     FSMEHSLAVKFGLKGIIRAQVATFKRLAWRVLQETGGITRREVDGFGYRMLVRSVLEE
                     HKDEFKLFRQAASKRGFTDQIEDLLKEFSRYCLDCYTMTDLHGVVAEAGAPRPLLDKA
                     DDLNLLMTKIEERLGTQYVDSEGHLALLASQIKHSELIKQADVYIDGFVTFTTREIEI
                     VAELMKHANRVTIALPMEDDLAGYADHHLFFNPVRTALHLRDLARAESVDIEEGVHFV
                     EPKRFMNEDLIHLEKEFDTYPTSEKAADGAVKVVEAADRRAEIHAVARNIRKLMMEGK
                     RYREMAILYRQPEVYDELIETIFPQYDIPVFISRKKPMLHHPLIEFSRSVLEAVTSGW
                     TYESIFRAVKTDLFFPLGDDVKLWRERADRLENFVLAQGIYGERWFDDTRWRVKRYRG
                     LELHTNVQTDEEKQMEQEIHLVRDLIREPLANFEARLKRAKNGRDVAEALFVFMEGLN
                     VYEKIVNLRIQEEKADQLLAATEHDQAWNEWINVLDQFVLMFGEKEEDPRQFIRILDE
                     GFDSLEFARIPPSIDQVTVMTVDISRLMHIDVVFVVGVTDGVLPKRIDQEGILSDTDR
                     EWFSQIGFALAPTTKERLMDETYMAYRAFTTPREKLFVSYPIADEEGKALIPSLYITR
                     IKQLLPGTETIPAVTDPSELPEGEDQ"
     gene            complement(2256..2882)
                     /locus_tag="VFX34_02035"
     CDS             complement(2256..2882)
                     /locus_tag="VFX34_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX5563714.1"
                     /translation="MEQWFDIDKILELSKEYRALGPLVGILFPFIESFLPFLPLFVFV
                     FANASAYGLWFGFILSWAGTVAGSYAVFLLIRKYGRNRLLRFLTRSKKVQKLIHWVER
                     NGFGPLFLLICFPFTPSALVNLVAGLSDMKKKRYLVTLMAGKFVMIFTISFIGHDLKA
                     LLTQPIRTAIVVLVIFFLWLIGKQFEHRLNKRVEEDFRSSKSRSKEER"
BASE COUNT          787 a          782 c          602 g          735 t
ORIGIN      
        1 ctggtcttca ccctctggaa gttcagatgg atcggtaacc gctggaatgg tctccgtacc
       61 cggcaataac tgcttgattc tcgtaatgta taaggacgga atgagcgcct ttccttcctc
      121 atctgcaatg ggatacgaga caaaaagctt ctcccgaggc gttgtaaacg cacggtaggc
      181 catataggtt tcatccatca gccgctcttt cgtagtcggt gccaacgcaa atccaatctg
      241 ggaaaaccat tcccggtcgg tatccgataa gattccttcc tgatcaatcc gctttggcag
      301 gacgccatcc gtaacgccaa cgacaaacac aacgtcaata tgcatcagcc gtgaaatgtc
      361 gaccgtcatc actgttacct gatcgatgga cggcgggatt ctggcaaatt ccaatgaatc
      421 aaatccttcg tccaagattc ggataaattg gcgcgggtcc tcctctttct ccccaaacat
      481 gagaacgaac tgatcaagaa cattaatcca ttcattccac gcctggtcat gctcggttgc
      541 ggcaagcagc tgatccgcct tctcctcctg gattcggaga ttgacgattt tttcgtatac
      601 attcagccct tccataaaga cgaacagcgc ttccgccacg tcacgtccat tcttcgcacg
      661 cttaagcctt gcctcgaaat tggcaagcgg ctcacgaatc aaatcacgga caaggtggat
      721 ttcttgttcc atctgctttt cttcatccgt ctggacattc gtatgtaact caagacctcg
      781 ataccgtttc acacgccaac gagtatcatc aaaccaacgc tccccgtaaa tcccctgtgc
      841 caacacaaag ttttcaagcc ggtccgctcg ttcgcgccat agcttgacat catctcctag
      901 cgggaaaaac aaatccgttt tcacggcacg gaaaatggat tcatatgtcc aaccagatgt
      961 caccgcttcc aggacggaac gggagaactc gatcagcgga tggtgaagca taggcttttt
     1021 cctgctaata aaaacgggga tatcatactg cgggaaaatc gtctcaatca gctcatcata
     1081 tacttccggc tgccggtaca agatggccat ctcccggtac cgtttccctt ccatcatcag
     1141 cttccggatg ttgcgggcaa ctgcgtggat ttccgcccgt cgatcggctg cttcgacgac
     1201 cttcacagct ccgtctgctg ccttttcaga cgtcgggtat gtatcaaatt ccttctccaa
     1261 atgaatcaga tcttcattca taaagcgctt cggctccacg aaatggacac cttcctcgat
     1321 gtctacggac tctgcacgtg caagatcacg gagatggaga gccgtccgca ccgggttaaa
     1381 gaataagtga tggtctgcgt accctgccag atcatcctcc atcggcaagg caatcgtcac
     1441 ccggttcgcg tgcttcatta attccgcaac aatttcaatt tcccgcgttg taaatgtcac
     1501 aaatccatct atatagacat ctgcttgttt gataagctct gaatgtttga tttgcgaagc
     1561 gagaagcgcc aaatgccctt cggaatcaac atattgcgtt ccaagacgct cctcgatttt
     1621 tgtcataagc aagttcaggt catccgcctt atcaagcaaa gggcgaggtg cccctgcttc
     1681 cgccacaaca ccatgcaaat ccgtcattgt gtaacaatcc aggcagtagc ggctgaattc
     1741 cttcaacaga tcctcaatct gatccgtaaa tccacgtttg ctggcagcct gacgaaacag
     1801 cttaaattca tccttatgct cttccaacac actgcggaca agcatccgat acccaaaccc
     1861 atccacttcc cggcgcgtaa tgcctcctgt ttcctgcaac acccgccaag cgagccgttt
     1921 gaaggtagcg acttgcgctc ggatgatccc ttttaatcca aacttgacag caaggctatg
     1981 ctccatggaa aaagacatct gatcaggtac aacaacaaaa atcggccgac cgcccggatc
     2041 tcgttccagt tcttccgcga cttccttttg aataaacatc gtctttccag taccggacct
     2101 accggtaata aaacgcaatg acataacggt tcctcctttg ccttgtcgtc tagttctagc
     2161 atctatgaaa aatgaacagt tgaaatgtaa aaatcctggc accctttctt tctattgtac
     2221 agaaagaaaa ggatgacagg aacaaaaaac atagcttagc gttcttcctt tgagcgtgac
     2281 ttggaggaac ggaaatcttc ctctactctc ttatttaatc tgtgttcaaa ctgcttacca
     2341 attaaccaaa ggaaaaagat gacgagtact acaatcgcgg tccgtattgg ttgtgtcaac
     2401 agtgctttta agtcgtggcc aataaacgag atcgtaaaaa tcataacgaa ttttcccgcc
     2461 atgagtgtga caagatatcg tttcttcttc atatcggata atccagcgac caggttgacg
     2521 agagccgatg gcgtaaacgg gaaacagatg agaaggaaca gaggaccaaa gccgtttctc
     2581 tctacccaat ggatcaattt ttgtactttt ttgcttctcg ttaaaaaacg tagcaaccgg
     2641 tttctaccgt atttacgaat caaaaggaaa actgcatagg atccggcgac tgttccagcc
     2701 caggagagga taaacccaaa ccataatccg tatgcactcg cattagcgaa aacaaagacg
     2761 aatagaggaa gaaacgggag aaaggattcg atgaacggga atagaattcc gaccaacgga
     2821 cctagcgcac ggtattcctt tgatagctca agtattttgt ctatatcaaa ccattgctcc
     2881 aaagtcgatc accctttacg tatgta
//
LOCUS       DASULI010000091         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1520879,
            whole genome shotgun sequence.
ACCESSION   DASULI010000091 DASULI010000000
VERSION     DASULI010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520879"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFX34_02040"
     CDS             <1..263
                     /locus_tag="VFX34_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX5563715.1"
                     /translation="RFTKEEYHQRFGLTVEREKMMKPGSIIMHPAPVNRDVEIAGSLV
                     EGRRSRIYKQVENGVYIRAAILEVLLKGRELTWRKSLRVPMY"
     gene            230..1528
                     /locus_tag="VFX34_02045"
     CDS             230..1528
                     /locus_tag="VFX34_02045"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX5563716.1"
                     /translation="MEKIIKSAYVLNEEGEMVLSDVRINGERITEIGTDLDTGNAEII
                     DADGLLLAPGFIDVHVHLREPGGEHKETIETGTLAAAKGGYTTICAMPNTRPVPDTKE
                     NLAHVNKLIEEKAHIRVLPYASITIREAGKERTNLKELKENGAFAFTDDGVGVQQAGM
                     MYEAMQDAAAIGMPIVAHCEDNTLIYGGAMHEGKRNKELGLPGIPSIAESVHIARDIL
                     LAEAAGAHYHVCHVSTKESVRVIRDAKKAGIHITAEVSPHHLILSEDDIPGDDADWKM
                     NPPLRGLEDLHALQEGLMDGTLDFIATDHAPHTAEEKSAGFAKAPFGITGFETAFPLL
                     YTHFVKEGKWSLKQLIEWMTEKPADVFGLPYGKIEVGAQADLVLLDLNKEQTIDRNTF
                     LSKGKNTPFDGWTCAGWPVKTIYGGNIVWQVGEVLQENSK"
     gene            1584..>2098
                     /locus_tag="VFX34_02050"
     CDS             1584..>2098
                     /locus_tag="VFX34_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5563717.1"
                     /translation="MPKRQDIETILVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYRV
                     ILINSNPATIMTDTEIADKVYIEPITLDFVSRIIRKERPDALLPTLGGQTALNMAIEL
                     QESGILDEYEIEIIGTNLEAIQKAEDRDHFRSLMKELDEPVPDSEIIHNLEEAYAFVK
                     KIGYPVIVRPA"
BASE COUNT          653 a          427 c          539 g          479 t
ORIGIN      
        1 caaggtttac gaaagaagaa taccatcaac gattcgggtt gacggttgaa agggaaaaaa
       61 tgatgaaacc tggatcgatc atcatgcatc cggcaccggt taaccgggat gtggaaatag
      121 cgggaagtct tgttgaaggc agacgttcaa gaatctataa acaagttgaa aacggcgtat
      181 acatacgtgc cgccatatta gaagtcttat tgaaaggaag ggaattgaca tggagaaaat
      241 cattaagagt gcctatgtat taaatgaaga aggtgaaatg gttctgtcgg atgtccgtat
      301 taatggagag aggataactg aaatcggcac tgatctggat actggaaatg ccgaaatcat
      361 cgatgcagat ggacttttgt tagcaccagg attcattgat gtccatgtcc atttgcgcga
      421 acctggcgga gagcataaag agacgataga aacaggaacg cttgcagcag ctaaaggcgg
      481 ttatacaacg atttgtgcaa tgccgaatac gagaccggtt ccggacacga aggaaaattt
      541 agcgcatgtc aataaattga ttgaagaaaa agcgcatata cgcgtacttc catacgcatc
      601 gatcacgatc agggaagccg gcaaagaacg gaccaatttg aaagagctga aggagaacgg
      661 ggctttcgca tttacggatg acggtgtcgg cgtccagcag gcgggaatga tgtatgaagc
      721 gatgcaggac gctgcagcga tcggcatgcc gattgtcgcc cattgtgaag ataatacact
      781 gatttacggt ggggcgatgc atgaagggaa gcgtaataag gagctcggtc ttccgggcat
      841 cccatccatt gctgaatcgg ttcatatcgc tcgcgatatt cttctggctg aagcagcagg
      901 cgctcactac catgtttgcc atgtgagcac gaaagaatcg gtacgggtta tccgcgatgc
      961 gaaaaaagcc ggcattcaca taactgcaga agtgagtcca catcatctga ttttatcgga
     1021 ggatgacatc cctggtgatg atgctgattg gaaaatgaac ccaccacttc gcggattgga
     1081 ggatttgcac gcattgcagg aagggttgat ggacgggaca ttggacttca tcgcgacgga
     1141 tcatgcacca cacacggcag aggagaaaag tgccggtttt gcaaaggcac cattcggaat
     1201 aactggattc gaaacggcgt ttccacttct ttacacgcac ttcgttaaag aaggaaagtg
     1261 gtcgctaaag caattgattg agtggatgac tgaaaagccc gcagatgtat tcggcctgcc
     1321 ttatggcaaa attgaagtcg gagcacaagc agatcttgtt ctcctggatt tgaacaaaga
     1381 acagacaatc gaccggaaca ccttcctatc aaaaggaaaa aacactcctt tcgatggctg
     1441 gacttgcgct ggctggcctg taaaaacaat ttacggtggg aacatcgtct ggcaggttgg
     1501 tgaagtactg caagaaaata gtaaataact ttaatttcac atttgcgtaa tgataaatgc
     1561 aagcaacgga aaggggaagt acaatgccaa aacgccagga tattgaaacg attctagtaa
     1621 tcggatccgg tccgatcatc atcggtcagg cggccgaatt tgactacgca gggacacagg
     1681 cctgcctttc attaaaggaa gaaggatacc gggtaatcct tattaattcg aacccggcaa
     1741 caattatgac ggataccgaa attgcagata aagtttacat cgaaccaata acgctcgatt
     1801 tcgtcagccg cattatccgc aaagaaagac cggatgccct tcttccgaca ctaggtggtc
     1861 aaacggcatt aaacatggct attgaacttc aagaatcagg gattttagat gaatatgaaa
     1921 ttgaaatcat aggtacgaat ctcgaagcaa tccaaaaggc agaagatcgt gaccacttcc
     1981 gttctctaat gaaggaactg gacgaaccgg ttcccgatag tgaaattatc cacaatttag
     2041 aggaagctta tgcgtttgta aaaaagattg gttacccggt aatagtccgc cccgcctt
//
LOCUS       DASULI010000092         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1716812,
            whole genome shotgun sequence.
ACCESSION   DASULI010000092 DASULI010000000
VERSION     DASULI010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716812"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2091
                     /gene="mutS"
                     /locus_tag="VFX34_02055"
     CDS             <1..2091
                     /gene="mutS"
                     /locus_tag="VFX34_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5563718.1"
                     /translation="TLIGEIEALGMKEIVVRERLHITLNEWMAKRDIVLSMENGEEDN
                     GSMNIQANVPSELNEACDILVSYLKRTQKSSLDHLRPFEYIQKSSKLSIDANSMRNLE
                     LVQSIRNGSKEGTLFWLLDETSTAMGARKLRIWIHQPLADKAAIEDRLTIVSDLIEDY
                     FLRDDLKTSLKEVYDLERLSGRISMGSASGRDLAQLRNSLRRVPHLKNALQQSGHERL
                     QRFSQQIEPCSEVLDLLEASIAENPPLSVREGGIIKDGYNEKLDQYRDASRNGKEWLA
                     GLEREERERTGIKNLKIGYNRIFGYFIEITKSNIHLADLSRYERKQTLANAERYITSE
                     LKEKEDLILNAEAEGQELEYTLFCTVRDEMKNHIRKVQRLASVLSELDVLLSFAEVSE
                     KQRFVRPQFHDGRALEIKNGRHPVVEKMMDHSLYVPNSCKLTEDANMQLITGPNMSGK
                     STFMRQVALTVIMAQIGCYVPCESALLPVTDQIFTRIGAADDLASGQSTFMMEMMESK
                     YAIANATGNSLLLFDEIGRGTSTYDGMSLAQAMMEFIHDEIGANTLFSTHYHELTKLD
                     LELERLVNVHVAAMEQEGRVVFLHKVMAGPADKSYGIHVAELAGLPDGIVVRAKELLD
                     AFESGEQAVAATAASEQLTFFDLQEEPDPIAEMDKSVLDQMKQTDLMNLTPMQAMNLI
                     LDWQKQLGSKEGTD"
     gene            2132..>3373
                     /gene="mutL"
                     /locus_tag="VFX34_02060"
     CDS             2132..>3373
                     /gene="mutL"
                     /locus_tag="VFX34_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX5563719.1"
                     /translation="MDIISVMDDSLSNKIAAGEVVERPASIVKELVENSIDANSTSIE
                     IALEEAGLTSIRVTDNGKGMSRRDAVLSFERHATSKISNEHDLFRIRTLGFRGEALAS
                     IASVSKVNLFTSDGESEGTELQLDGGVVVKQSNAAFRKGTDMTVSHLFYNTPARLKYM
                     KTIQTELGHTIDLVNRIALSHPHIAFKLSHHSQTILHTSGSGDRRKVLTDIYGMAVAR
                     KMVPFAGENADFKVDGYVTLPEMTRASRNYMTLIVNGRWVKSYAANQAVLDGLHTYLP
                     IGRFPIAVINVETDPFLTDVNVHPSKQHIRLSKEGELFDLIKSSVRDAVKRSIIVPDA
                     VKREPVKRIPSEQVTIWNEFQPASVRKETEVDHRRLEVEQKEPRFDTNLFEKKPLQTV
                     YEPAKQEPVQTIQPEKVMEVHE"
BASE COUNT          988 a          727 c          874 g          784 t
ORIGIN      
        1 acattgattg gcgaaatcga agcgcttgga atgaaagaaa ttgttgtcag agaacgattg
       61 catatcacgt tgaatgaatg gatggcaaaa agagatattg tcctttcaat ggaaaatgga
      121 gaagaggaca acggttccat gaacattcaa gcaaatgttc ctagtgagtt gaacgaggca
      181 tgcgatattc ttgtttccta tttgaaacgt acgcaaaagt cctcgttgga ccatttacgt
      241 ccatttgaat atatacagaa atccagtaaa ttatcgatcg atgccaactc gatgcgcaat
      301 cttgagcttg tccaatcgat caggaacgga agcaaagaag gaacgctttt ctggctattg
      361 gatgagacgt caactgcgat gggcgcaaga aaattaagaa tatggatcca ccagcccctt
      421 gccgacaaag ccgcgattga ggacaggctt acaatcgtgt ccgacttgat cgaggattat
      481 tttcttcgcg atgatttgaa gacatcattg aaggaagtat atgaccttga acgattgtcg
      541 ggaaggattt ccatgggtag tgcaagcgga agggatttgg cgcagctgcg aaattcactt
      601 cggcgggtgc cccatttgaa aaatgcactg cagcagtccg gccatgaacg actgcaacga
      661 ttttcccaac agattgagcc ttgttcggaa gtcctcgatt tattggaggc ttcgattgct
      721 gaaaatccac cgctttctgt cagggaaggc ggtatcatca aagacggcta taacgaaaag
      781 ctggatcagt acagggatgc ttcaagaaac ggcaaggaat ggctagcggg actcgaacgc
      841 gaggaacggg agcggacagg catcaaaaac ttgaaaatcg gttataaccg tattttcggc
      901 tatttcattg aaattacgaa atccaatatc caccttgctg atctaagccg ttatgagcgc
      961 aaacagacgc ttgctaatgc ggagcgctat atcacttccg agctgaaaga gaaggaagat
     1021 ttgatcctga acgccgaagc ggaaggtcag gagctggaat acaccctgtt ctgcacagtg
     1081 cgcgatgaaa tgaaaaatca tatccggaaa gttcaacgac tggccagcgt tctttctgag
     1141 cttgacgtgc tcctttcttt cgcggaagtg tccgaaaagc aacggttcgt cagaccgcaa
     1201 ttccatgacg gacgggcatt ggagattaaa aacggaagac atccagtcgt agagaagatg
     1261 atggatcatt cattatacgt tccgaatagc tgcaaattga cggaagatgc caacatgcaa
     1321 ctgattacag gtccgaacat gtccggtaaa agtacattca tgcggcaagt cgccttgacg
     1381 gtcatcatgg cccaaatcgg ctgctatgtg ccttgcgagt ccgcattgct tcctgtgacg
     1441 gatcagattt tcacaaggat tggtgcggcg gatgatcttg catccggcca aagtacgttc
     1501 atgatggaaa tgatggaatc gaaatatgca attgcaaatg caaccggaaa cagtttgctc
     1561 ctctttgatg aaatcggcag gggaacatca acgtatgacg gaatgtcgct tgctcaggca
     1621 atgatggagt tcatccatga tgaaattggg gcaaacacat tgttttcgac ccattatcat
     1681 gagctgacaa agcttgacct ggaattggag aggcttgtga atgtccatgt tgctgcgatg
     1741 gaacaagaag ggagagtcgt cttcctccac aaggtgatgg cggggccggc ggataaaagt
     1801 tacggtatcc atgttgctga gctggccggt cttccggatg gaatcgtagt ccgtgcgaaa
     1861 gagctgctcg acgcttttga aagcggagaa caggcggttg ctgcaaccgc ggcatccgaa
     1921 caattgacgt tcttcgactt gcaggaagag cccgatccga tagcggaaat ggacaagtcc
     1981 gttttagatc agatgaaaca gacagattta atgaacctga cgccgatgca ggcgatgaac
     2041 ttgatccttg attggcaaaa acaacttggc tcaaaggaag gaacggactg aaactattta
     2101 cgtatgatca acggagataa ggagggattc gatggatatc atttcagtca tggatgattc
     2161 attatccaat aaaattgctg cgggagaagt cgtagagcga ccggcctcga ttgtgaaaga
     2221 actagtcgag aactcgattg acgctaatag cacatcaatt gaaatagcgc ttgaagaagc
     2281 ggggctcact tcgattaggg tgaccgataa tggaaaggga atgtcgcgtc gcgatgctgt
     2341 cctttcattt gaacgccacg caacaagcaa aatatcgaat gaacacgact tgttccgtat
     2401 cagaacgctc ggattcaggg gggaggcgct cgccagtatc gcttccgtat ccaaggtcaa
     2461 cctcttcact tcagacgggg aatcagaagg gacagagctg cagctggacg gtggagttgt
     2521 tgttaagcaa tccaacgccg cattccgaaa ggggacggac atgacggttt cccacctttt
     2581 ttataacacg cccgcgaggt tgaaatatat gaagacgatc cagacggagc ttggacatac
     2641 gatcgatctt gtcaaccgga ttgcgcttag ccatccccat atcgctttca aattgtccca
     2701 tcatagccaa acgatactgc atacatccgg cagcggagat cgccggaagg tcctcactga
     2761 tatttacgga atggcggttg cacggaaaat ggtgcccttt gcaggagaga atgccgattt
     2821 caaagtggat ggatacgtca ctctgcccga aatgacaaga gcatccagaa actatatgac
     2881 tctcatcgtc aatggaagat gggtgaaaag ctatgcagca aaccaagccg tgctcgatgg
     2941 acttcacacc tatttaccga tcggccgctt tccaattgcc gtcatcaacg ttgaaacgga
     3001 cccatttttg acagacgtaa atgtacaccc ttccaaacag catattcgcc taagcaaaga
     3061 gggagagctc tttgacctga tcaaatcatc tgttcgtgat gcggtgaagc gatcaataat
     3121 tgttccggat gctgtgaagc gggaacctgt caaacgaata ccttccgagc aagtaacgat
     3181 ttggaatgaa ttccagccag cgagcgttcg gaaggaaacg gaagttgacc acaggagatt
     3241 ggaagtggag cagaaagagc ctaggtttga tacgaatcta tttgagaaga aaccgctcca
     3301 aacagtttat gaacctgcca agcaagaacc tgtgcaaacg attcagcctg aaaaagtaat
     3361 ggaagtccat gag
//
LOCUS       DASULI010000093         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1622187,
            whole genome shotgun sequence.
ACCESSION   DASULI010000093 DASULI010000000
VERSION     DASULI010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622187"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..967
                     /locus_tag="VFX34_02065"
     CDS             236..967
                     /locus_tag="VFX34_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007201358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX5563720.1"
                     /translation="MRSANPSLGEDTFKGLRADGGMSMTISGTVNKTFILFLLLCVTA
                     FFTWHQYFAGNDIQALLWIGLGGGFVVALITIFVKKAAPYTAPLYALLEGLVIGGISA
                     MFESQFEGITTQAILLTLGTLFSLLLAYRSGFIKVTENFKLGVVAATGAIFFVYLADF
                     VLRFFGMEVPFIHETGIVGILISVAIVVVAALNLVLDFDFIEKGAEQHAPKYMEWYGA
                     FGLMVTLVWLYLEILRLLSKLRSNK"
     gene            complement(1138..2505)
                     /gene="rlmD"
                     /locus_tag="VFX34_02070"
     CDS             complement(1138..2505)
                     /gene="rlmD"
                     /locus_tag="VFX34_02070"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018661655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5563721.1"
                     /translation="MVVDIDHLDGKGNGQAVVWRENELGNKRKLKLIIPQSLPGETVQ
                     VTVDDPTMRWQKVLPDEILAANPDRMEAPCPHFELCGGCVWQHWSYEGQLKHKTEHVK
                     KALESVGMDPGLVEDTIGMDEPWHYRNKMEFTFSPEGDLGLHEQGNFRSIIPLETCLI
                     AGRNMVDATMEVSGWVKEFGLSGYNKDTREGLLRHLMVRESFETGEVMLALFATEAPA
                     GELAAAVEDLIERITKNFPNVKSLMWLVNTDIADRTQSEETFVLAGRDFIYDELGGYR
                     YRLWFDTFFQTNPVQAQKLVDLALEMGNPQEDEKMIDLFCGVGTFSLPFAARVKELAG
                     IEIVESSIESAKRNAIDNDLHNTTFLARDARRGIDEMHESWGLADMLLIDPPRCGAGG
                     KVMRRIGRAQPKRIVYVSCNPDTFATDTAELLQFGYTLQKVQPVDLFPHTVHVECVAL
                     LTLNN"
BASE COUNT          559 a          655 c          629 g          663 t
ORIGIN      
        1 tgcaagttct tcaaatgaca gacgcaaccg ttccagcatc atggcaatcc cccaattctt
       61 atacttcatt tcagtaaatc ttttgttttg ttttcaagtc caatgatcat caattatacc
      121 aatatacaga cagtaatggt gcgaagtcga aaaacttgaa accaaaccct ttctcccccg
      181 tatactctac tatcaatcta tttcacacgc ctgaattaaa ggaaggggat gcatgatgag
      241 atcagcgaat ccgagtttag gagaagatac gttcaaaggg ctgcgggcgg acggcggcat
      301 gtcgatgacc atttcaggga ctgtgaacaa gacgttcatc ctgtttttat tgctatgtgt
      361 gacggccttc ttcacgtggc atcagtactt tgcagggaat gatattcaag cgcttctttg
      421 gatcggcctc ggcggtgggt ttgtcgtcgc gctcattacg attttcgtga aaaaggcggc
      481 tccttacaca gcaccgcttt atgcgctgtt ggagggactc gtgatcggcg ggatttcggc
      541 gatgttcgaa tcccagttcg aagggatcac gacgcaggcg atcctgttga cgctaggaac
      601 gttgttttca ttgttgcttg cgtaccggtc ggggttcatc aaggtgacgg agaacttcaa
      661 gcttggggtt gtcgctgcga cgggggcgat cttctttgtg tacttggccg atttcgtcct
      721 ccgcttcttc ggcatggagg ttccgttcat ccatgagacg gggattgtcg gcattctgat
      781 ttcggtcgca atcgtcgttg tcgcggcatt gaatctcgtg ctcgattttg atttcatcga
      841 aaagggtgcg gagcagcatg cgccgaagta tatggagtgg tacggcgcat tcggcctcat
      901 ggtcacgctt gtatggctgt atttagagat tctcaggctg ctttcgaagt tgcggagcaa
      961 taaataggca agaaattaag gtgcctgtgt agcacacatt tatgacaatt caataagagt
     1021 tatagtaata cagaaagaag ccaaccgaat gttgatatgg ttggcttctg tttttatttt
     1081 atgcaattgt agtgcatagt ctgaattgtt cgttgcacag gcacccaaac aattcaatca
     1141 attattcaac gtcaacagtg caacgcattc gacatgcacg gtgtgcggga agaggtcgac
     1201 cggctgcact ttttggagtg tgtagccgaa ctgaagcaat tccgctgtat ccgttgcgaa
     1261 tgtgtcgggg ttgcatgata catagacgat ccgcttcggt tgggcgcggc cgatccggcg
     1321 cattactttg ccgccagcac cgcagcgcgg cgggtcgatc agtaacatgt ccgccaagcc
     1381 ccagctttca tgcatttcat cgattccccg gcgcgcatcg cgggcgagga atgtcgtgtt
     1441 atgcaaatca ttgtcaatcg cattccgttt tgcggattca atcgacgact cgacaatctc
     1501 gatgccagcc aattccttca cgcgcgcggc gaatggcagt gagaatgtcc cgacgccgca
     1561 aaacaggtcg atcatttttt cgtcttcctg cggattgccc atctccagcg caaggtcaac
     1621 cagcttctgc gcttggacag ggttcgtctg gaagaacgtg tcgaaccaga ggcggtagcg
     1681 atagccgccc aattcatcat agatgaagtc gcggcctgcg agcacgaacg tctcctcgga
     1741 ttgcgtacgg tctgcgatat ccgtgttcac gagccacatc aagcttttca cattcgggaa
     1801 gttcttcgtg atccgttcaa tcaaatcttc gacagcagcc gccaattcac cggcaggcgc
     1861 ttctgtcgcg aacaatgcaa gcatgacttc accggtctca aacgattcac ggaccatcag
     1921 atggcggaga agcccttcac gcgtatcttt gttatagccg gacaggccga actccttcac
     1981 ccatccgctc acttccatcg tcgcatcgac catgttccgg cctgcgatca ggcaagtttc
     2041 aagcgggatg atgctgcgga aattcccttg ctcatgcagc ccgagatccc cttccggcga
     2101 aaaggtgaat tccattttat tgcggtaatg ccaaggctcg tccatgccga tcgtgtcctc
     2161 gacgagaccc ggatccattc cgacgctttc caacgctttc ttgacatgtt ccgtcttatg
     2221 cttcagctgg ccttcgtaac tccaatgctg ccagacacag ccgccgcaaa gctcgaaatg
     2281 cgggcaaggc gcttccatgc ggtctgggtt cgcagccagg atctcgtcag gcaatacctt
     2341 ctgccatctc atcgtcggat catccacagt cacttgcacg gtctcccccg gtaatgattg
     2401 cggaatgatg agcttcagct ttctcttgtt gcctaattca ttctcacgcc agacgaccgc
     2461 ttgaccattt cctttcccat ccaaatggtc gatatcgaca accatt
//
LOCUS       DASULI010000094         7612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_9798, whole
            genome shotgun sequence.
ACCESSION   DASULI010000094 DASULI010000000
VERSION     DASULI010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9798"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /gene="ftsZ"
                     /locus_tag="VFX34_02075"
     CDS             complement(<1..897)
                     /gene="ftsZ"
                     /locus_tag="VFX34_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5563722.1"
                     /translation="MLDFDTNIDSLAVIKVIGVGGGGNNAVNRMIEHGVQGVEFIAVN
                     TDAQALNLSEAEVKLQIGAKLTRGLGAGANPDVGKKAAEESREQIEEALRGADMVFVT
                     AGMGGGTGTGAAPVIAQVAKDLGALTVGVVTRPFTFEGRKRQTQAIGGIAAMKESVDT
                     LIVIPNDKLLEIVDKNTPMLEAFREADNVLRQGVQGISDLIAVPGLINLDFADVKTIM
                     SNKGSALMGIGMSTGENRAAEAAKKAISSPLLETSIDGAKGVLMNITGGSNLSLFEVQ
                     EAADIVASASDEDVNMIFGSVIN"
     gene            complement(939..2222)
                     /gene="ftsA"
                     /locus_tag="VFX34_02080"
     CDS             complement(939..2222)
                     /gene="ftsA"
                     /locus_tag="VFX34_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5563723.1"
                     /translation="MSQSTIYVSLDIGSSSVKVLIGEVDGGSLHVIGVGNVQSAGIRK
                     GTIIDIDATVQSIRKAVEQAERMTGMQIREVILGIPANGVHLQDVKGVVAVNSENREI
                     TDDDLDRVMKSAQVMSVPPEREIVNIIPKQFIVDDYDEIKDPRGMIGVRLEMDGTLIT
                     TSKTLVHNILRCVERAGLVIREIYLQPLAAGQFALTEDEMNHGTAFIDIGGGSTTIAI
                     FGDNRFISTAVIPVGGDHITKDLSIILKTPTEQARKIKHQYGHAFYDDASDDELFEVP
                     VIGADSKDQYSQRYISEIIGVRLEELFDLVLEELYRMGIQDLPGGVVLTGGTTKLDGI
                     LQLARHVLRTRVRIHTPEYIGVREPMYSTAVGLIRYASMEDSYFGTVAESQTVATNSE
                     MDYQPSSKKKVAVRPKEKKTSFMSKTKKFFDGFFE"
     gene            complement(2317..2688)
                     /locus_tag="VFX34_02085"
     CDS             complement(2317..2688)
                     /locus_tag="VFX34_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEX5563724.1"
                     /translation="MWLPLLGLLLGVSLGLLSDLHIPQIYSNYLSIAVLAALDTLFGG
                     IRAQLQQIYDDKVFISGFFFNIVLAAGLAFLGVHLGVDLYLAAVFAFGVRLFQNIAVI
                     RRILLTKWSDRSKVSETKVQE"
     gene            complement(2691..3410)
                     /locus_tag="VFX34_02090"
     CDS             complement(2691..3410)
                     /locus_tag="VFX34_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX5563725.1"
                     /translation="MKKSINWKFTIVLLLIGFMIAVQYNTMEHPKERDTRDIWAIRQE
                     LAEEKKIHSELLTEIRELDRTISSYRTLSESSTGQALRDTVDKLHKKAGTTDISGPGF
                     TIEVRPSVESIAYGIPITSISSDLLTRFVNEVNRFKGIHMEIDGKRYTMMSSIRVING
                     ATTVNGLNVSTPPFTIKIISPTMSESEKLYNYLLASSIHDDFYLDNLVLEINQPEQDV
                     TVRGYPDKFKNLYLHELPKGE"
     gene            complement(3436..4161)
                     /locus_tag="VFX34_02095"
     CDS             complement(3436..4161)
                     /locus_tag="VFX34_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX5563726.1"
                     /translation="MKLAKKMSEDYKTRGRYILYSFVFLILGFIFAFSYRTLGKDSGE
                     SAQSPAFHQEEQYREELIAQKERNKELADEIHAKQDEIRDFEKSFSENEDNHTVLVEE
                     AKDLRLLLGIIPASGSGVRVTLKDGDYDPVEQNPNDYIVHESHVLRVVNELKISGAQG
                     IAINGQRISANSYIKCTGPVITIDGRTLPAPFVIEAVGDTDVLSAALNLGGGVIDGLV
                     HDNIVVTLEQLKEIKLPALRDEA"
     gene            complement(4136..4930)
                     /locus_tag="VFX34_02100"
     CDS             complement(4136..4930)
                     /locus_tag="VFX34_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX5563727.1"
                     /translation="MDKVIDIEERIPSMREKRRRKTNKKFLFIIAVFCIALLVLLYFQ
                     SPFSKVGEVKIQGAALHDEQYYYEKSGILEGASFWGFETKDVEEELLKLEGVSNVAVT
                     RKWMRDIDISIDEWKTVAYMESGGEYGLLLENGSIFPSDGNYPNVPILNHVDNPDLRK
                     RLTNQLLKMDKNVLQLISEIIYTGTEENPDTIVVYMDDGYEVHAILQTFAEKMDYYPD
                     IISQLGDHEKGIVNMEVGIFFTPFSKVYGKADVEEGEGDEVGEENE"
     gene            complement(4945..6036)
                     /gene="ftsW"
                     /locus_tag="VFX34_02105"
     CDS             complement(4945..6036)
                     /gene="ftsW"
                     /locus_tag="VFX34_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX5563728.1"
                     /translation="MRSLDRKLKVAFVISAVSLSLIGLVFVHSAGAYWGQVHYADSSP
                     FIVKQGIYMAVSIFVAFMLMKGSWTSSESFWTAFYYITILLLVAVMIPGIGAVRNGSQ
                     SWIALGPFSIQPAEFVKVALIGKLACNMKQSAGKNPFKLSHFVLILLPAALIMLQPDL
                     GSAVIMIVSAFVILFLAGYPLTFFGILGFAGIGAFVALIAAAPYRLDRIKSYIDPWND
                     PLGAGFQGIQSLFAIAPGGLFGHGYGNSRQKYLYLPEPQNDFIFSIIAEETGFIGATF
                     ILVLFSVLLLSAFGIAIRAHDYKSFLMTAGMASMIIFQTFLNVGVVSGLLPVTGVTLP
                     FISYGGSSLLTTWMAAGTILHFAFYRQRS"
     gene            complement(6077..7426)
                     /gene="murD"
                     /locus_tag="VFX34_02110"
     CDS             complement(6077..7426)
                     /gene="murD"
                     /locus_tag="VFX34_02110"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5563729.1"
                     /translation="MGKRDEFLGKKVLVLGLAKSGYAAADLLHLLGAVVVVNDSSPET
                     GNVEAQTLRSKGISVICGGHPATILDEGFDFIVKNPGIPYHNPVIQQAERKGIPIWTE
                     IELSYLVSEAPIIAITGSNGKTTTTTLLYHILNIGGKKPLIAGNIGTVACTVAQNAKS
                     DEVIVMEVSSFQLAGTVNFRPKIAVWTNLYDAHLDYHGTSEEYALAKAKVTDNQTEED
                     FFIYNADQPEMQPYINRSAARKIPFSLQGKQAEGISADDEMIYWQGEPFVKRSIIKLL
                     GRHNLENILAATASAILMDCDKETIENVLSSFTGVRHRMQFVKELKGRTFYNDSKATN
                     TLATKSALSSFQSPVVLIAGGLDRGHSFEELRPYMSRVKAVLALGETADRLLDFAASC
                     GVEHAVKADSMEEAVQEAFSISLEGDQILLSPACASWDQYPNFEERGDAFIDAVMKL"
     gene            complement(7427..>7612)
                     /locus_tag="VFX34_02115"
     CDS             complement(7427..>7612)
                     /locus_tag="VFX34_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5563730.1"
                     /translation="IETLSVMIQVTSFKLTGKRVFKMSPIHHHFELSGWSEWKIVLVF
                     WGVALFAAMIPVLWEVM"
BASE COUNT         2020 a         1987 c         1492 g         2113 t
ORIGIN      
        1 attgatgaca gaaccgaaaa tcatgttgac gtcttcatct gaagcggaag caacgatatc
       61 cgcagcctct tgaacttcga acaagctcaa gttggatcca cctgtaatat tcatcaacac
      121 gccttttgct ccgtcgatcg acgtttccaa aagcggactt gaaattgctt tctttgcagc
      181 ttccgccgca cggttttcgc ctgtggacat accaattccc atcagcgcgg aacctttatt
      241 cgacatgatc gtttttacgt ctgcaaagtc aaggttgata agaccaggga cagcgatcaa
      301 gtcggaaatg ccttgcaccc cttgacggag aacattgtcg gcttcccgga acgcttcaag
      361 catcggcgtg ttcttatcaa cgatttccaa taatttatca ttgggaatga caatcagcgt
      421 atcgactgat tctttcattg ccgcaattcc gccaatcgct tgcgtttgac gttttcttcc
      481 ttcaaatgtg aatggacgtg taacgacacc taccgtcaaa gctccaagat cctttgccac
      541 ttgtgcgatg acaggtgctg cccctgttcc agttccaccg cccatacccg cggtaacgaa
      601 caccatatca gcgccgcgga gcgcctcttc tatctgctcc cggctctctt cagccgcttt
      661 ttttcctaca tcggggtttg cacccgcacc tagtccgcga gtcaattttg ctccgatttg
      721 caattttact tcggcttcgg ataaattaag cgcttgcgcg tccgtattca cagcgatgaa
      781 ttccacacct tgtacaccat gttcaatcat gcggttaacc gcgttatttc ctccgccacc
      841 gactccaatc accttgatga ccgccagtga atcgatattt gtatcaaaat ccagcatgct
      901 ctctcctcct aatccctata tgaccatatt tagaccagtc actcaaaaaa tccatcgaaa
      961 aatttctttg ttttgctcat gaagcttgtc tttttctctt taggtcggac ggcgaccttc
     1021 tttttactgg aaggctgata gtccatctcg ctgttcgtag cgacagtttg agattccgcc
     1081 actgtcccaa aatagctatc ttccatacta gcataacgga taagacctac ggcagtcgaa
     1141 tacatcggtt cacgcactcc gatatattca ggtgtatgga tgcgcaccct cgttctcaat
     1201 acatgtcttg cgagttgtaa aatcccatcg agcttcgtcg tgccgcctgt caatacgacg
     1261 ccgcccggca aatcttggat acccatacga tagagctctt ccaggaccaa gtcgaatagt
     1321 tcttccaatc ggacaccaat gatctccgat atgtatcttt gactatattg atctttcgaa
     1381 tcggcaccga tgacaggtac ttcaaaaagt tcatcatctg atgcatcatc gtaaaaagca
     1441 tgtccatatt gatgcttgat ttttcttgcc tgctcagttg gtgtcttcag aatgatcgac
     1501 aagtcctttg taatatggtc cccgcccact ggtatgacag cagttgaaat gaagcggtta
     1561 tccccgaaga tggcaatggt cgtcgaacct ccgccaatat cgataaatgc cgtgccatgg
     1621 ttcatttcat cctcagtcaa cgcaaactgt ccggctgcga gcggctgtag atagatttca
     1681 cggattacaa gacccgcccg ttcgacgcat cgcagaatat tatgcactaa cgttttggaa
     1741 gtcgtaatca gtgtaccgtc catttcaagg cggacgccga tcattcccct tggatctttt
     1801 atttcatcat aatcatccac aatgaattgc ttcggaataa tatttacgat ttcccgttca
     1861 ggcggaaccg acatgacttg cgcggatttc ataacccgat ccaggtcatc atcggtgatt
     1921 tccctgttct ccgaattgac ggctacaacg cctttgacgt cttgaagatg cacaccattt
     1981 gccgggatgc ctagaatgac ctcccggatc tgcatgcctg tcatccgttc agcctgctcc
     2041 accgctttgc ggatggattg gacagtcgca tcaatgtcga taatcgtccc tttacgaatg
     2101 cctgcagatt ggacgttacc taccccgatt acgtggaggg atccgccatc cacttcgccg
     2161 atcagcactt tgactgacga agatcctata tctagtgata cataaattgt agattgactc
     2221 aattcccggc acctcccatt tcagtcctta tcttacattc tattgtattc gagtcgtatt
     2281 gtctaaatat tttctccatc cgatgaccaa ttcctttcat tcctgaacct tcgtttcaga
     2341 aactttactt cgatctgacc atttcgtcag caaaatcctt ctaatgacag cgatattctg
     2401 gaataacctt actccaaatg cgaatacggc agccaaatat aagtccactc cgagatgcac
     2461 accgaggaaa gccaatcctg ccgctagtac gatattgaag aagaatccgg aaatgaacac
     2521 tttatcatca taaatttgtt gcagttgcgc ccgtatccct ccgaaaagcg tatccaatgc
     2581 agctaacacg gcgatggaca aatagttgct gtaaatttgc gggatatgca gatctgataa
     2641 cagacccagc gaaactccga gtagtagacc gagcagtggc aaccacattg tcattcccct
     2701 ttcggaagtt catgtaaata aagattttta aatttgtcgg gatacccccg gacagtcacg
     2761 tcctgttccg gctgattgat ttccagtaca agattatcaa ggtaaaaatc gtcgtggatg
     2821 gacgatgcca acagataatt atacagtttc tcactctctg acattgtagg agaaattatt
     2881 ttaatcgtaa agggcggggt cgagacattc aatccattca cggtggtcgc gccattaatg
     2941 acccgtatcg aactcatcat cgtataccgt ttgccatcga tctccatatg aatgccttta
     3001 aagcggttca cttcgttgac gaaacgggtc agtaaatcgg atgaaatact cgtgattggt
     3061 attccatacg cgatactttc caccgaaggc cggacttcga ttgtaaaacc aggtcccgaa
     3121 atatccgtcg ttcccgcctt tttgtgcagc ttgtccacag tgtccctcag cgcttgtcct
     3181 gtgctcgatt cacttagcgt tctgtaagaa gaaatcgttc tatccaattc acggatttcg
     3241 gtaagaagct cagaatggat tttcttctct tctgccaact cttggcgaat tgcccaaata
     3301 tccctcgtat cacgttcctt aggatgttcc atcgtattat attgaacagc aatcataaat
     3361 ccaattagca ggaggacaat tgtgaatttc caattgatag attttttcaa cgagcaccct
     3421 ccctcttact attcattatg cttcatccct taatgcagga agtttgattt ctttcaactg
     3481 ttccaatgtg acgacgatat tgtcatgtac caatccatcg atcaccccgc cgcccaagtt
     3541 caatgctgcc gacaggacat ctgtatcgcc taccgcctca atgacgaacg gagccggaag
     3601 cgttctgccg tcaattgtga tgacagggcc tgtacatttg atatatgaat tagccgaaat
     3661 gcgctgccca tttatggcaa tcccttgcgc gccggaaatc ttcaattcat ttacaactcg
     3721 gagtacatgg ctttcatgga cgatataatc attcggattt tgttcgaccg ggtcatagtc
     3781 gccatctttc aaagttacac gcaccccaga accggaagca ggaatgatgc ctagaagcaa
     3841 tctgagatcc tttgcctcct cgacaagcac ggtatgatta tcttcattct ccgaaaagga
     3901 tttctcaaag tcccggattt catcttgctt tgcatggatt tcatcggcaa gctccttatt
     3961 tctttccttt tgcgcaatca actcttcccg atattgctct tcctgatgaa atgcagggga
     4021 ttgtgcggat tcgccgctgt ccttccctaa cgttcgatag gagaacgcga agatgaaacc
     4081 gagtatgagg aaaacgaaag agtataatat gtatctccct ctagttttat agtcctcact
     4141 cattttcttc gccaacttca tcaccttccc cttcctctac atcggctttt ccgtaaactt
     4201 tgctaaaagg tgtgaagaaa atgcccactt ccatattgac aatccctttt tcatgatccc
     4261 ccaattggga aatgatgtcc ggataataat ccatcttctc tgcaaaggtc tgaagaatcg
     4321 catggacttc atatccatca tccatataaa caacaatcgt atcgggattc tcctctgtcc
     4381 cagtatagat gatttcggaa atcaattgca gaacattttt atccattttc agcaattggt
     4441 ttgtcagtct ttttcgcagg tcagggttgt ccacatgatt taaaatcggc acgttcgggt
     4501 aattcccatc acttgggaaa atcgacccgt tttcaagcaa taaaccatat tccccgccac
     4561 tttccatata ggcaacggtt ttccactcat ctatggaaat atcgatgtcc ctcatccatt
     4621 ttctcgtgac ggcaacatta ctgacgcctt caagcttcaa aagttcttct tctacgtctt
     4681 ttgtttcaaa tccccaaaat gacgcccctt ctaatatccc acttttttca taatagtatt
     4741 gttcatcatg aagcgcagcc ccttggattt tcacttcccc taccttacta aaaggagatt
     4801 ggaaatataa aagcacaagg agggcgatac agaatacggc tatgatgaat aaaaacttct
     4861 tattcgtctt ccttctccgt ttttccctca tggatggtat cctctcttcg atgtctatca
     4921 ctttatccat gtgccgccct cctctcaact tcgttgtcta taaaaagcga aatgtaaaat
     4981 agtgcccgca gccatccaag tcgtaagcaa tgatgagccg ccataactga tgaacggcaa
     5041 tgtcacacct gttacaggca ataagcccga gacgaccccg acattcagga atgtttgaaa
     5101 gatgatcatt gaggccattc cggctgtcat caaaaatgat ttgtaatcat gcgcacggat
     5161 cgcaatgccg aacgcggaca gaaggaggac tgaaaatagg acgagaatga atgtcgctcc
     5221 gataaaaccc gtctcctcag caatgatcga aaatatgaag tcattttgcg gttccggcaa
     5281 gtacaagtat ttctgcctgc tattgccata tccatgacca aacaatccac ccggcgcaat
     5341 tgcaaataat gactggatcc cttgaaagcc cgctccgagc ggatcattcc acggatcgat
     5401 atacgacttg atgcgatcca gacgataggg tgctgcagcg ataagggcaa cgaacgcccc
     5461 gattcccgca aagccgagaa tcccgaaaaa tgttagcgga tatccggcca aaaacaaaat
     5521 gacgaaagcc gaaacgatca tgatcaccgc agaaccaaga tcgggctgaa gcatgatcaa
     5581 tgcggcaggc aataatataa gcacaaaatg gcttagtttg aatgggttct tccccgcgga
     5641 ttgtttcatg ttgcaagcaa gctttccaat aagcgccact ttgacgaatt ccgccggctg
     5701 gatgctgaac ggaccgagtg caatccaact ttgggatccg ttccttactg caccgatacc
     5761 tggaatcata accgcaacca acaacaggat cgttatgtaa taaaaggcgg tccaaaaaga
     5821 ttcactggat gtccatgatc ctttcatcaa cataaaagct acaaatatag aaacagccat
     5881 gtaaatccct tgcttgacga taaaggggga tgaatccgca tagtgcacct gcccccagta
     5941 cgcacctgcc gaatggacaa aaaccaaacc tatcaatgac aatgacactg ccgaaatgac
     6001 gaaggcaact tttaatttcc gatccagtga acgcatcctt ccgtcttatt ggtaagtccc
     6061 agtgtgaacg aattacttaa agtttcatga ctgcatctat aaatgcatcc ccacgctctt
     6121 caaaattcgg atattgatcc cagcttgcac atgccggcga cagcaggatc tgatcgcctt
     6181 caagggaaat tgaaaatgcc tcttgaacag cttcctccat cgagtccgct ttcactgcat
     6241 gttcaacccc gcatgaagct gcaaaatcga gaagcctgtc agcagtttcc cctaatgcaa
     6301 gaacagcttt caccctgctc atgtacggtc tcagttcctc gaacgaatgc cctctatcca
     6361 atccgccggc aatcagtaca acgggcgatt ggaacgaaga taaggcactt tttgtcgcca
     6421 aggtgttcgt cgctttcgaa tcgttataga acgtcctgcc tttcaattct tttacaaact
     6481 gcatgcgatg ccggactccc gtgaaagagc ttaacacatt ttcgattgtt tccttatcac
     6541 aatccattag aatcgccgat gcagtggcag ctaaaatatt ctccagatta tggcgtccga
     6601 gcaatttgat gatcgatctt ttcacaaacg gctcgccttg ccagtagatc atttcatcat
     6661 ctgccgaaat cccttccgct tgcttccctt gtaaggaaaa cggaattttc cgggcagcag
     6721 accgattaat atacggttgc atttcaggtt gatccgcatt gtatataaag aaatcctctt
     6781 cggtctgatt atctgtcacc ttcgcttttg ccaaggcgta ttcttccgac gttccatgat
     6841 aatcgagatg tgcatcatat aaatttgtcc agacagctat cttcggacgg aagtttaccg
     6901 ttccagccaa ctggaaagag gatacttcca tgacaatcac ttcatcagat tttgcattct
     6961 gtgcgacagt acacgcaaca gtcccgatat ttcctgcaat caaaggcttt ttgcctccaa
     7021 tattcaaaat atggtacaga agcgttgtcg ttgttgtctt accgttcgat cctgtgatgg
     7081 cgatgatcgg tgcttcactt accaaataag aaagctctat ttcagtccaa atcggaatcc
     7141 ccttccgttc cgcttgctga atgactggat tgtgataggg gatgccggga tttttgacga
     7201 taaaatcaaa gccttcatcc aaaatagttg ccggatgccc gccgcaaata actgaaatcc
     7261 cttttgaccg caaggtttgt gcttccacat taccagtctc gggagatgaa tcattgacga
     7321 ccacaaccgc cccaagcaaa tgcagaagat ctgccgcggc atagccgctc ttggcaagac
     7381 cgagcacaag aaccttcttg cccaagaact catctcgctt tcccatttac atcacctccc
     7441 acagtacggg tatcattgcg gcaaataacg ccactcccca aaaaacgaga acaattttcc
     7501 attccgacca tccggacaac tcaaaatggt gatgaatcgg actcatttta aatacccgtt
     7561 tcccggtcaa tttaaagctt gtcacttgga tcattaccga caatgtttcg at
//
LOCUS       DASULI010000095         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1697809,
            whole genome shotgun sequence.
ACCESSION   DASULI010000095 DASULI010000000
VERSION     DASULI010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697809"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VFX34_02120"
     CDS             complement(<1..1485)
                     /locus_tag="VFX34_02120"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX5563731.1"
                     /translation="MRLLERLKDNVLVADGAMGTLLYSYGIDFSYEELNVEKPEIIEK
                     IHREYIDAGADVIQTNTYGANSLKLARYGLESRVKEFNEAAIRIAKHAAAPGNQYVLG
                     TIGGLRGIRKSDASLAEVLTIVREQADALLSGDPDGLLLETYYDFEELSTVVTELRKL
                     TDVPLIAQVAMDEPGVLQNGMHLNDALHQLESLGADIVGVNCRLGPFHTIQAFENVTI
                     PERAFLSAAPNASLLDVEDGRIVYASEADYFGRAATFLREEGVRLIGGCCGTTPKHIA
                     AAKKQLTGVPPVIEKTVSPQKPIIIREAEPAKQVPLHIKAKTERTVIVELDTPRHLET
                     EQYFEGAKQLHATGIDAVTLADNSLASPRISNMAMGSMLKHEHHIRPLVHLTCRDHNL
                     IGLQSHLMGLDALGIHDILAVTGDPTKVGDFPGATSVYDVSSMELLQLIKQLNEGISF
                     TGKPLRKKAKFSVAAAFNPNVRVLDRAVQRLEKKIECGADYFISQ"
     regulatory      complement(1751..1857)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1972..2157
                     /locus_tag="VFX34_02125"
     CDS             1972..2157
                     /locus_tag="VFX34_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5563732.1"
                     /translation="MRIFKFAVPIALLVAGISWWMLSKNFQEVPGTSRVYITIGAAIL
                     SGIIAHFLFPKSEDKER"
BASE COUNT          567 a          592 c          525 g          563 t
ORIGIN      
        1 ctggctaatg aaataatccg caccgcattc gattttcttt tcgagccgct gtaccgcgcg
       61 gtcaaggacg cggacgttcg ggttgaacgc tgcggcgaca gagaatttcg cctttttgcg
      121 gagcggtttg cctgtgaatg aaatcccttc attcaattgt ttgatgagct gcaagagttc
      181 catgctggag acgtcataga cgctcgttgc cccggggaag tcgccaactt tcgtcgggtc
      241 gcccgtcacg gcaagaatat catggattcc gagcgcatcg agtcccatta gatgggattg
      301 cagtccgatc aggttatggt cgcggcacgt caaatggacg agcggccgga tatgatgctc
      361 atgcttcaac atcgatccca tcgccatatt gctgatacga ggggaggcga gtgagttatc
      421 cgccaacgtg acggcatcga tcccggtggc atgcagttgt ttcgcgcctt cgaagtattg
      481 ctccgtttcc aagtgacgag gtgtatcaag ctctacgatg accgtccgct ccgtcttcgc
      541 ttttatatgg agcggcacct gttttgccgg ctccgcctcc cggatgatga tcggcttctg
      601 cggagaaacg gttttctcta ttactggcgg cactcctgtc agctgctttt tcgccgcggc
      661 gatatgtttc ggcgtcgttc cgcagcatcc accgatcaga cggacgcctt cttcgcggag
      721 gaatgttgcc gcgcgaccga aataatcggc ttcggacgca taaacgatcc ggccatcctc
      781 gacatctagc aagcttgcat tcggggcggc ggacaaaaat gcccgctccg gaatcgtcac
      841 attttcaaat gcctgtatcg tatggaaagg accgagcctg caatttactc cgacaatatc
      901 tgcaccaagc gattcgagct gatgcagggc atcattgaga tgcattccat tttgcaacac
      961 accgggctca tccatcgcga cttgcgcaat aagcggcaca tctgtcagct tccgaagctc
     1021 ggtgacgacc gtcgacagct cttcgaaatc ataatacgtt tcaagcagca atccatctgg
     1081 atcgccggaa agcaaagcat ccgcctgttc gcgcacgatt gtcagcactt cggcaagcga
     1141 agcatcgctt ttccggatgc ccctcagccc gccgatcgtc ccaagcacat actgattgcc
     1201 gggtgctgca gcatgcttcg caatccggat cgccgcctcg ttgaattcct tcacacggct
     1261 ctccaatccg taacgggcca gtttcagcga gttggcgcca tatgtattcg tctggatgac
     1321 atccgccccg gcgtcgatat actcgcggtg gatcttctcg atgatttcag gcttctcaac
     1381 gttcaactct tcatagctga agtcgatgcc atatgaataa agcagcgtcc ccatcgcccc
     1441 atccgccacc agtacattat ctttcaaccg ttcaagcaat ctcataagaa atcatccttt
     1501 ctccattcga tataatgcat gcaaaaacgc ataaatccca taaatgacgc ataaatcctt
     1561 aaagtcacgc ataaacaccg gcagatgcgc ataaatccgg aaatctacgc ataaacgact
     1621 ccaaaagcgc ataaaacccg aaattcacgc atatcttgca tttcccatac aaaaaattta
     1681 gacagaattg aaaaataaaa aagccttcgc tgaaaaaaga aggctttgaa tatgcgatgt
     1741 ggctcgctcc ttctcatctg ccaggccgat agcgacctgc tggaattagc acctgaccga
     1801 taacggctgg ttgctgaagc ttcgacgggc cagtccctca gcttctcttg ataagaaatt
     1861 gttatgcagt tgtttgaata atttaaaaca tcatatcgtt aattgtttga tacgtcaata
     1921 gccgaattgt gatagaatcg gcatatgaac aaaaagtaga aggtgaatac gttgaggatt
     1981 ttcaaatttg ccgttccgat cgcactcctt gtcgccggga tttcatggtg gatgctctct
     2041 aaaaactttc aggaagttcc agggacatcc agagtgtata ttacaatagg cgcagccatt
     2101 ctaagcggga tcattgccca ttttttattt ccgaaaagcg aagacaaaga acgttgatac
     2161 aaagaccgat agccaaataa gctgtcggtt tttttattcc gcagcgatgg ttaacccaaa
     2221 atgaaaatag gttgacacaa tttgagt
//
LOCUS       DASULI010000096         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_677621, whole
            genome shotgun sequence.
ACCESSION   DASULI010000096 DASULI010000000
VERSION     DASULI010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677621"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..503)
                     /locus_tag="VFX34_02130"
     CDS             complement(57..503)
                     /locus_tag="VFX34_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX5563733.1"
                     /translation="MLIEKIIGNIGDREEIPGKKIEWVELEWEELSKRILRTETTEGT
                     DIALRIDQEEPLKYGDLLFEDDNRAVVIRTKMEPVIVIRPKDMVEMGKAAFELGNRHT
                     PSLIEKDEIIVRADHTLAPLLDEVGVDYETTERRFKQPFKYRGHSH"
     gene            complement(754..2469)
                     /gene="ureC"
                     /locus_tag="VFX34_02135"
     CDS             complement(754..2469)
                     /gene="ureC"
                     /locus_tag="VFX34_02135"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX5563734.1"
                     /translation="MRITHEQYAKMFGPTVGDKVRLADTDLWIEIEKDYTTYGDEGIF
                     GGGKSLRVSMGQNGEKTSEDGVLDTVITNATIIDHTGIVKADIGIKNGRIAGIGKAGN
                     PNTMDGVDQGMIIGVGTEVYAGEGLIATAGAIDTHIHLISPQQVDVALNAGTTTFIGG
                     GTGPAAGSTATTVTPGKWHLHRMLQQIEDIPLNIGIFGKGSAAFPAPLIEQIRAGAIG
                     MKIHEDWGATPSALDQSLTIADEYDVQIALHSDTLNEAGFVEDTINAINGRVIHVFHT
                     EGAGGGHAPDQLKMASLPNVLPASTNPTKPFTVNTIDEHMDMLMVCHHLKRDVAEDVA
                     FADSRIRPETIAAEDIMQDMGILSIMSSDSQAMGRVGEVAIRTWQTADKMKKQRGPLP
                     QDEGKDNDNYRVKRYISKYTINPAIAQGISHEVGSIEDGKLADIVLWDPAFFGVKAQV
                     VIKGGIAVYAMTGDPNASIPTPQPMMGRRMYGYHGQGPQNAAMTFLPKIAVEEGLPEE
                     LGLKKMIGTVQNCRNVSKADMVHNSEMPVIDVDPETYEVKIDGELATCEPVDVLPMAQ
                     RYFLF"
     gene            complement(2470..2799)
                     /gene="ureB"
                     /locus_tag="VFX34_02140"
     CDS             complement(2470..2799)
                     /gene="ureB"
                     /locus_tag="VFX34_02140"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX5563735.1"
                     /translation="MEINVGRDTKTVKVANTGDRPIQVGSHFHFIEVNKFLEFDREHA
                     VGMHLNIPSGTAVRFEPGEEKEVELVEFGGRRHVFGLNKLTEGSTHKKDEIVEKASDG
                     GFKGADS"
BASE COUNT          602 a          823 c          613 g          762 t
ORIGIN      
        1 atgtgccgat cgggaatgcc gaatcgtgga tttggagcag acgtaaaaat tgtatatcag
       61 tgggaatggc cgcggtattt gaaaggttgt ttgaatcgtc gttcagttgt ttcatagtcc
      121 acacccacct catccaataa tggcgccaac gtgtgatctg cgcggacgat gatttcatct
      181 ttttcgatta gtgaaggagt atggcggttg ccaagctcga atgcggcttt gcccatttcg
      241 accatatcct tcgggcggat gacgatgacc ggttccattt tcgtccggat cacaacggcg
      301 cgattgtcat cttcgaacaa tagatcgccg tatttcaacg gctcttcctg gtcgattcgc
      361 aatgcgatat ccgtcccttc cgtcgtctcc gtccgcaaaa tccgcttgct caactcttcc
      421 cattccagtt cgacccattc gattttcttt cctgggattt cttctctatc accgatattg
      481 cctatgattt tttcgattag catcttgcat ttctccttct ttgcaaagac tcctgacaac
      541 tgttgatttc cgttccaggc ggacgctccg tgagcctagt cgaacagcga agcgaagggt
      601 tcgataggcc ttatcccgct ccctttgggt cacaaaaaac attgctcgca gcgctgtgct
      661 tttgctcgca aacgccgttc tatgtgacgg cgttcgcatg agtcaccgcc ttacactcca
      721 atcaactaac tacctgaaat taattataaa gaattaaaac aggaaatacc gttgtgccat
      781 cgggagtacg tcgactggtt cgcatgtggc gagttccccg tcgattttca cttcatatgt
      841 ctccggatcg acgtcgatga ccggcatttc gctattgtgc accatatccg cttttgatac
      901 gttgcggcaa ttttggaccg ttccgatcat cttcttaaga ccaagctctt ccggaagtcc
      961 ttcttccact gcaattttcg gcaggaaggt catcgccgca ttttgcggac cttgtccatg
     1021 gtagccgtac atcctgcggc ccatcatcgg ctgcggtgtc ggaatggaag cattcggatc
     1081 acctgtcatc gcgtagacag cgatgccgcc tttaatgacg acttgcgctt tgacgccgaa
     1141 aaatgccgga tcccataaaa cgatatccgc caatttcccg tcctcgatcg aaccgacttc
     1201 atgtgaaatc ccttgggcga ttgcagggtt gatcgtatat tttgagatat aacgcttgac
     1261 gcggtaatta tcattgtctt ttccttcatc ttgcgggagc ggaccgcgct gcttcttcat
     1321 cttatccgct gtctgccatg tccggatcgc cacttcaccg acccggccca tcgcttggga
     1381 gtcggaagac atgatactta aaatgcccat gtcctgcatg atgtcctcag ccgcaatcgt
     1441 ctcgggacgg atacgcgaat ccgcgaatgc gacatcctcc gccacatcac gtttcaaatg
     1501 gtggcacacc atcaacatgt ccatatgctc gtcaatcgta ttgaccgtaa atggcttcgt
     1561 cgggttcgtc gacgccggca atacattcgg caatgacgcc attttcaact ggtcaggcgc
     1621 atggcccccg cctgctcctt ccgtatggaa tacgtggatg acccgtccat tgatcgcatt
     1681 gattgtatcc tcgacgaatc ctgcctcgtt caacgtatca gagtgcaagg cgatctgaac
     1741 gtcatactca tccgcaatcg tcaagctttg atccaatgcc gacggagtcg ccccccagtc
     1801 ctcatggatt ttcatgccga ttgcgcctgc ccggatctgt tcaatgagag gcgcagggaa
     1861 agccgcgctt ccctttccga atataccgat attcaacgga atgtcttcaa tttgctgaag
     1921 catccgatgc aaatgccatt tccccggtgt cactgtcgtc gcagtggaac ccgccgcagg
     1981 gcctgtcccg ccgccgatga atgtcgtcgt accggcattc agcgccacgt ccacttgctg
     2041 cgggctgatg agatggatat gcgtgtcaat tgcacctgct gtcgcaatca acccttcacc
     2101 cgcatatact tcggtgccga caccgatgat catgccctgg tcgacgccgt ccatcgtatt
     2161 cgggtttccc gccttcccga tgcctgcaat cctgccgttc ttgattccga tatccgcctt
     2221 cacgatccct gtatgatcaa tgattgtcgc attcgtaatg actgtatcca acacgccatc
     2281 ttctgacgtc ttttcgccgt tctgccccat cgacacgcga agggacttcc cgccgccaaa
     2341 aataccctca tccccgtacg tcgtataatc cttctcgatt tcaatccata aatcggtatc
     2401 cgccaaacgg accttgtcgc ccacagtcgg gccgaacatc ttagcgtact gctcgtgtgt
     2461 aattctcact taactgtcag ctcctttaaa tccgccatca gacgccttct caacaatttc
     2521 atctttttta tgagtggaac cttccgtcaa tttattcaaa ccaaatacat ggcgccttcc
     2581 gccaaactca acgagctcca cttccttctc ctcgcccggc tcaaaacgta cagccgtacc
     2641 ggatggaata ttcaaatgca tgccgaccgc atgttcacga tcgaactcga ggaacttatt
     2701 cacctcgatg aaatggaaat gagacccgac ttgaatcggc cggtcacctg tattcgcaac
     2761 cttgaccgtc ttcgtatcac gtcccacatt gatttcaatc
//
LOCUS       DASULI010000097         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1598704,
            whole genome shotgun sequence.
ACCESSION   DASULI010000097 DASULI010000000
VERSION     DASULI010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598704"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFX34_02145"
     CDS             complement(<1..577)
                     /locus_tag="VFX34_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014459159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5563736.1"
                     /translation="MEDLLKLKGKNIVVMGVANERSIAWGVAKSLFDVGANVIFTYRK
                     ERSLAKLEKALESNGLEPKMIVQCDVNEDESIRNAFTSIGEALGTIHGIVHSIAFAHA
                     EDLKNDFVETTRSGYAFAQDSSSYSLVATAREARRFMTEGGSIITMSYLGAERVLDGY
                     NVMGVAKAALEASVRYLANDLGKEAIRVNAIS"
     gene            777..1811
                     /gene="argC"
                     /locus_tag="VFX34_02150"
     CDS             777..1811
                     /gene="argC"
                     /locus_tag="VFX34_02150"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX5563737.1"
                     /translation="MKIGIIGATGYGGLELLRLLHNHPEVEQLDLFTSSDEGTSFSVK
                     FPHTTNLYDQPLLKIDYEKSTGYDIIFASTPAGVTSRLLPPLIGNGPKLIDLSGDFRL
                     KDTMEYERWYKKTAAPIEGIEQSAYGLTEWNSEAIQSAQLIANPGCYPTAVLLSLLPL
                     LKERLIDPSALIIDAKSGVSGAGNTPGPMTHFSETNENFTIYKIHEHQHIPEIEQGIE
                     QFGAQKTTVTFTTHLVPMTRGIMATSYAPVNAGVTEEQLVSCLKETYAGRPFVRVMNE
                     STKFCTKQVAGSNYCDIHVKVDPRTNRATIVSVIDNLVKGAAGQAVQNMNIQFGLDET
                     TGLTQVPLFI"
     gene            1826..>2144
                     /locus_tag="VFX34_02155"
     CDS             1826..>2144
                     /locus_tag="VFX34_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX5563738.1"
                     /translation="MAVAVQQLKRISGKNIATPKGFSAVGIHCGLKHKKHDLAVLLSE
                     VPASVAGVFTTNAIQAAPLLVTKEVVYQTGKMQAVIVNSGNANACTGKQGMIDALTMQ
                     EKTA"
BASE COUNT          618 a          495 c          499 g          532 t
ORIGIN      
        1 cggaaatcgc attcacacgg attgcttctt tccccaaatc attcgccaag tagcggacgg
       61 acgcctcaag cgcagccttc gcaacaccca ttacattgta cccgtcaagc acgcgttcag
      121 cgcctaaata gctcatcgtg atgattgaac cgccttcggt catgaaacga cgagcttctc
      181 tagctgtcgc gaccaacgaa taggagcttg aatcttgtgc gaatgcataa ccgcttctcg
      241 ttgtttcaac gaagtcattc ttcagatcct cagcgtgcgc aaaagcgatc gagtgaacga
      301 ttccatggat tgtgccaagc gcttctccga ttgaagtaaa tgcattccgg atgctttcgt
      361 cttcattcac atcgcattgg acgatcattt tcggttcgag tccgttgctt tccaatgctt
      421 tctcaagctt ggcaagtgaa cgttccttgc gatatgtgaa aatgacattc gctcctacgt
      481 caaaaaggga cttcgctacg ccccatgcaa tgcttcgctc gttcgccaca cccattacga
      541 caatattctt tcctttaagc ttcaataaat cttccatagt gacctcctca aacggtagaa
      601 atctatcacc taatattagt acctgataat aactatagta tagcatatcc ttcttcaatt
      661 caaaactcca attctcgaac aggtgaatta aaattcgaca caaaatttat attaatacag
      721 ttgactttat atttatgcat acgtatacta attctaacga ttaaaggagt ggggatatga
      781 agataggaat cataggtgct accggctacg gcggacttga attgctccgc ttgctgcaca
      841 atcacccgga agtggaacaa ctcgatttat tcacatcgtc agatgaaggg acatcgtttt
      901 ccgttaaatt tccgcataca acgaacttgt atgatcagcc tcttctgaaa atcgattatg
      961 agaagtcgac cggctatgac atcatttttg ccagtacgcc agccggagtg acaagtcgat
     1021 tgctgccgcc gctcatcggc aatgggccga agctgatcga tttatcggga gacttccgtt
     1081 tgaaggacac aatggaatat gaacgttggt ataagaaaac agctgcacca attgaaggaa
     1141 tcgagcaaag tgcatacgga ttgacggaat ggaattcgga ggcaatccaa agcgcacagc
     1201 tgattgcgaa ccccggctgc tatccgaccg cggttctcct ttctttattg ccattattaa
     1261 aagaaagact aatagatcca tcagcactta tcatcgatgc gaaaagcggt gtatcaggag
     1321 ccgggaatac acctggaccg atgacacatt tcagtgagac gaatgaaaac ttcaccatct
     1381 ataaaatcca cgagcaccag catatcccgg aaattgaaca aggaattgaa cagtttggag
     1441 ctcaaaaaac aacagtgaca tttacaacac atcttgtccc gatgactagg ggcattatgg
     1501 cgacaagcta tgcgcctgta aacgccggcg ttacggaaga gcagcttgtc agttgcttga
     1561 aggaaacata tgcgggaagg ccatttgtga gggtgatgaa tgaatccaca aagttttgca
     1621 cgaagcaagt ggcgggctcg aattactgtg atatccatgt gaaagtggat cctcggacga
     1681 atcgggctac aattgtttca gtcatcgata acttggtgaa gggggctgcc gggcaggcgg
     1741 tccaaaatat gaatatacaa ttcgggttgg atgaaacgac aggtttgacg caagttccat
     1801 tattcattta agaggaggaa ttggtatggc ggtagcagtt caacaattga aacgcatttc
     1861 aggaaagaat atcgcaacac cgaaagggtt cagcgcggtc ggtatccatt gcggactgaa
     1921 acataagaag cacgacttgg cggtattgct cagtgaagtc cctgcaagcg tggcgggtgt
     1981 ttttacgacg aacgccatcc aggcagctcc tcttctggtt acaaaggaag ttgtgtacca
     2041 gaccgggaaa atgcaagcgg tcatcgtcaa ttccggcaac gcgaatgcat gcaccgggaa
     2101 gcaggggatg atcgatgcac ttacgatgca agaaaagacg gccg
//
LOCUS       DASULI010000098         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1204634,
            whole genome shotgun sequence.
ACCESSION   DASULI010000098 DASULI010000000
VERSION     DASULI010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204634"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..942)
                     /gene="minD"
                     /locus_tag="VFX34_02160"
     CDS             complement(145..942)
                     /gene="minD"
                     /locus_tag="VFX34_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEX5563739.1"
                     /translation="MGEAIVITSGKGGVGKTTTSANLGTALALQGKKVCLVDTDIGLR
                     NLDVILGLENRIIYDLVDVIEGRCKIHQALVKDKRFEDGLFLLPAAQTTDKNAVEPEQ
                     MKELITELKRDYDYILIDCPAGIEQGYKNAVAGADRAIVVTTPEISAVRDADRIIGLL
                     EQEDIEPPKLIINRIKSRLVNSGDALDVNEITTHLSIDLLGIVLDDEKVISSSNKGEP
                     VVMDPSNPAAIGYRNIARRILGESVPLMSLDEGKPGFFGKLKTLFAK"
     gene            complement(944..1615)
                     /gene="minC"
                     /locus_tag="VFX34_02165"
     CDS             complement(944..1615)
                     /gene="minC"
                     /locus_tag="VFX34_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEX5563740.1"
                     /translation="MTKQHYVTIKGTKDGLVLRLDDKCAYSDMIAELRNKVSENSLEG
                     LAEVKVHTGNRFCNEEELKEIMNVVHESPHLRVSKIQSEVISIEECNRRVLENQSETY
                     IGIVRSGQVVKADGDLVVIGDVNPNGRVVAAGSIYILGRLKGIAHAGATGNEEAVIAA
                     SWLEATHLMIADKLETMTDELTILSEQPEMECAYIHQNGFIAIDRLQELRILRPNLST
                     FKGGS"
     gene            complement(1640..2158)
                     /gene="mreD"
                     /locus_tag="VFX34_02170"
     CDS             complement(1640..2158)
                     /gene="mreD"
                     /locus_tag="VFX34_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5563741.1"
                     /translation="MIRFIIPLIAVILFFLEPVFSLFSPISIADVSYTLVPRFVIVYL
                     IFITAYYGRRRAILYGIVLGLLYDMYHIDIIGVYTFLYPVICYLASLIIRQVHRHIIS
                     VMVLSLAMVALLELLSFFFASLIALTAIGFEEFIVLRLMPTMVANSIFVGMFGYLFKR
                     LIDEFGLQKAGI"
     gene            complement(2155..3051)
                     /gene="mreC"
                     /locus_tag="VFX34_02175"
     CDS             complement(2155..3051)
                     /gene="mreC"
                     /locus_tag="VFX34_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5563742.1"
                     /translation="MPRFFSNKRLILLLVGVIVLVALISFSLKDRQNATLPEQIVKDV
                     VGFGQSIFSKPAHFITGTLDKIDGILNTYEENKHLKARLKEYASTQAELADVKADNKK
                     LREIIDKKDSLRDYEPIQATVISRNPDQWEEKIVIDKGSVAGVKLNRAVITASGLIGK
                     VVLVTPFKSTVELLSTENSNFRVAAVIPGEEAIFGLIEGYDPARGELIMKRIDSNFDV
                     KEGQKVESSGLGGIFPKGLLIGEVTEVSTDDFGLTKLAYIRPAADFSMLDHVMVANRS
                     TGVTSGTDGEVDGEEEEGEDGS"
     gene            complement(3070..>3797)
                     /locus_tag="VFX34_02180"
     CDS             complement(3070..>3797)
                     /locus_tag="VFX34_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5563743.1"
                     /translation="WKKPNVMICVPYGITSVEQRAVTDAAKQAGAKDAFTIEEPFAAA
                     IGANLPVWEPTGSMVVDIGGGTTEVAVISLGGIVTSESIRVGGDSMDGAIIGYIRKMY
                     NMTIGERTAEAIKIEIGSAKVSAKPEKMEIRGRDLLTGLPKTLEISSDEIVEALRESI
                     TQIIEGVRKTLEATPPELSADVMERGIVLTGGGALLENLDKVISDETNMPVFIAEDPL
                     DCVAIGTGKALDNFEVVKKMQAR"
BASE COUNT          986 a          979 c          777 g         1055 t
ORIGIN      
        1 tgacccgccc ggaacggaaa tcaacgtcgc gtcagtctat cgtttttgca tcaatgcgtc
       61 taataagaca aaagggatgg gaatcaattc gatttccatc ccttttgtct acagtctgaa
      121 aaacccctta gtaagggttc ctggttattt cgcgaataat gttttcagtt tcccgaaaaa
      181 accgggtttt ccttcgtcta gtgacataag aggcacggat tcgcctaaaa tacggcgggc
      241 gatgttccgg tagccgattg ctgccgggtt cgaaggatcc atgacgacag gctccccttt
      301 attcgaagag gagatgactt tctcatcatc gaggacgatt ccaaggagat caatcgataa
      361 atgagtcgtg atttcattga catccagagc atcgccgcta ttgacgagac ggcttttgat
      421 ccgattgatg atcagtttcg gaggctcgat atcctcctgc tccaacaatc cgatgatccg
      481 atccgcgtcc ctcactgccg aaatttcagg tgttgtgaca acgatggccc ggtccgcgcc
      541 tgcgacggca ttcttatatc cttgctcgat gcctgcaggg cagtcaatca aaatatagtc
      601 gtagtcccgc ttcaattcag taatcaattc cttcatttgt tccggttcga ctgcattttt
      661 atctgtagtc tgggcagccg gaagtaagaa gagtccatct tcaaaacgct tgtccttgac
      721 gagtgcctga tgaattttgc atcgtccttc tattacatcg acgagatcat aaatgatacg
      781 gttttcaaga ccgaggatga cgtccagatt acggaggccg atatccgtat caacaagaca
      841 taccttttta ccttgtagag ccaatgctgt cccaagattt gcagaagtcg tcgttttacc
      901 gactccacct tttccagacg ttattacgat tgcttcaccc acactagctc cctcctttga
      961 atgttgacag atttggccgc agaatccgta gctcctgtaa acggtcgatg gcaatgaagc
     1021 cgttctgatg tatgtatgcg cattccatct ccggttgttc cgacaaaatc gtcagttcat
     1081 cagtcatcgt ttccaactta tccgctatca tcaaatgcgt cgcttcaagc cacgaagccg
     1141 caatgaccgc ctcttcattg ccagttgcac ctgcatgtgc gattcctttt aatctaccta
     1201 atatataaat gcttcccgca gccactacac gaccgttcgg attaacgtcg ccgatgacga
     1261 caagatctcc gtccgccttt acgacttgcc ctgagcggac gattccgata tatgtctccg
     1321 attgattctc aagaaccctc cgattgcatt cttcgatcga aatgacttca ctttgaatct
     1381 ttgaaacacg taaatgtggc gattcgtgta cgacattcat gatttccttc agttcctctt
     1441 cattacaaaa gcggtttccg gtatggacct ttacttctgc aagcccttcc agactgtttt
     1501 cgctgacttt attgcggagt tctgcaatca tgtccgaata agcgcatttg tcgtcaagtc
     1561 ggaggacaag cccgtctttt gtccctttta ttgttacata gtgctgtttc gtcatttgcg
     1621 tccctcacag tttccttgat tatattccgg ctttttgcag tccgaattca tcgatcagcc
     1681 gtttgaacag atagccgaac atccctacaa agatggaatt ggcgaccatt gtcggcatga
     1741 gccgcaatac gatgaattct tcaaaaccga ttgcggtcag tgcaatcagg ctcgcgaaaa
     1801 agaatgacag caattcaagc agcgcaacca tcgccaacga taaaaccatg actgagatga
     1861 tatggcgatg gacttgccta atgatcaaag aggcaagata acaaatcacc ggatataaaa
     1921 aagtatatac tccaataata tcaatgtgat acatatcata caagagtcca agtacgattc
     1981 cgtaaaggat tgcgcgtctg cggccatagt aggctgtgat gaaaatcaga taaacgataa
     2041 caaaacgggg aacgagcgtg tatgatacgt cagcgatcga tatcggtgag aaaaggctga
     2101 ataccggttc gaggaaaaat aatatgacag caataagagg gattatgaac ctgatcacga
     2161 tccatcctcc ccctcttctt cctcaccgtc gacttcgcca tccgttccgc ttgtgacgcc
     2221 agttgaccgg tttgcaacca ttacatgatc caacatggag aaatcagcgg ccggacgaat
     2281 ataggccaat ttcgtcaatc cgaaatcatc cgttgaaact tcggtcactt ccccgatcag
     2341 caatcctttc gggaaaatac cgcctaatcc agaagattcg actttctgtc cttccttgac
     2401 gtcaaaattc gaatcgatgc gcttcattat caattcgcca cgtgctggat catatccttc
     2461 tatcaaaccg aaaatcgctt cttcaccagg aatgactgca gctacccgga agttgctgtt
     2521 ttcagtcgac agcaattcaa ctgtcgattt aaaaggagtg acgagcacca cttttccaat
     2581 cagccctgat gcggttatga cagccctgtt caacttcaca cctgcaacgc tgcctttatc
     2641 gatgacaatt ttctcttccc attgatcggg attccgggaa atgacagtcg cctggatcgg
     2701 ctcgtaatca cggaggctat ctttcttgtc tatgatttca cgcagctttt tattatccgc
     2761 tttgacatct gccagctccg cctgagtgga agcatattct ttcaatcgcg ctttcaaatg
     2821 cttgttttct tcatatgtat tcaaaatacc gtcgatttta tccaatgtgc ctgtaataaa
     2881 atgtgccggt ttcgagaaaa tcgattgccc gaaaccgacg acatctttca caatttgctc
     2941 aggaagggtg gcgttctgcc tgtcttttaa ggagaaggaa atcaatgcca ctaggacaat
     3001 gacgcccaca agcagcaaaa tcaaccgttt attcgaaaaa aatcgcggca ttgcccaaat
     3061 cctccctcat tacctagctt gcatcttttt gaccacttca aaattatcga gcgctttccc
     3121 cgttccgatc gctacacaat caagcggatc ttcagcgatg aagacaggca tgttcgtttc
     3181 atccgaaatg accttgtcca agttttcaag caatgctccc ccgccagtca agacgatccc
     3241 tcgttccatg acatccgcag aaagttcagg aggagtagct tccaacgttt ttctgacacc
     3301 ttctataatt tgcgtgatgg attcacgaag cgcttcgacg atttcgtcag aagagatttc
     3361 caatgttttc ggaagacctg tcaaaagatc tcgccctctg atttccatct tttcaggttt
     3421 agcggaaact ttcgctgaac cgatttcaat cttaatcgct tccgcagtac gttcaccgat
     3481 tgtcatatta tacattttac gaatataacc gataattgcg ccgtccatgg aatcaccgcc
     3541 gacacggatt gactcgcttg taacgatgcc gccgagcgaa atgactgcaa cctcggtcgt
     3601 accgccacct atatcgacga ccatactgcc cgtaggttcc caaacgggta aatttgcacc
     3661 aattgcagcc gcaaagggct cttcaatcgt gaatgcatct ttagcacctg cttgtttagc
     3721 tgcatccgtc acagcacgtt gttcgacaga agtaatcccg taaggaacac agatcataac
     3781 attcggtttt ttccatg
//
LOCUS       DASULI010000099         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1016536,
            whole genome shotgun sequence.
ACCESSION   DASULI010000099 DASULI010000000
VERSION     DASULI010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016536"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="VFX34_02185"
     CDS             <1..1878
                     /locus_tag="VFX34_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX5563744.1"
                     /translation="LEHLREEENKLKWEGTFAEYLDILRERKEVAQTAHSRVYEMIKS
                     HGETKENGYTKFRFFENEIYGLEDTIERLVNEYFHPAAKRLDVRKRILLLMGPVSGGK
                     STIVTLLKRGLEQYSKTDEGAVYAIKGCPMHEDPLHLIPDYLRDDFFHEFGIRIEGSL
                     SPLNTMRLEKEYDGRIEDVLIERIFFSEDKRVGIGTFTPSDPKSQDIADLTGSIDFST
                     IAEYGSESDPRAYRFDGELNKANRGLMEFQEMLKLDEKFLWHLLSLTQEGNFKAGRFA
                     LISADELIVAHTNETEYRAFISNKKNEALHSRIIVMPIPYNLKVSQEEKIYEKMIRES
                     DMAHVHIAPHALRVAAIFSVLTRLKISKKQGIDIVKKMRLYDGENVEGFNTADLEELK
                     KEFPNEGMDGIDPRYVINRISSAIIRKEVDAINALDVLRSLKEGLDQHASISKDDKEE
                     YLNYISIARKEFDEIAKKEVQKAFVYSYEESARTLMDNYLDNVEAFCNKHKMKDPLTG
                     EEMNPDEKLMRSIEEQIGISENAKRAFREEILIRISAYARKGKRFDYKSHERLREAIQ
                     KKLFADLKDVVKITTSSATPDETHLKKINEVVARLIDEYGYNSISANELLRYVGSLLN
                     R"
     gene            1957..>3036
                     /gene="yhbH"
                     /locus_tag="VFX34_02190"
     CDS             1957..>3036
                     /gene="yhbH"
                     /locus_tag="VFX34_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HEX5563745.1"
                     /translation="MHEEQSESFVVSQENWSLHRKGYQDQKRHLDKVKKAIHNNLPEL
                     ISEESIVMSDGRDVIKIPIRSLDEYKIRYNHNKSKHVGQGSGDSQVGDVVASDGSQSP
                     GKGKQAGDQPGQDYYEAEVSIAEIEETLFKELELPNLEKREQAEITEEDIQFNDIRKK
                     GLIGNIDKKRTIIAAFKRNAMEGHADIMPIYNDDLRFKTWDEVVKPQSKAVVLMMMDT
                     SASMGTFEKYMARSFYFWMTKFLRTKYEKVDIEFIAHHTIAKVVTEVDFFSKGESGGT
                     ICSTAYEKALDLIAQKYNPSRYNIYPFHFSDGENMSSDNGKCMSLVEELMEVSNMFGY
                     GEVNAHSRYSTLMNTYKKIEDPKFRH"
BASE COUNT          957 a          610 c          775 g          694 t
ORIGIN      
        1 ttggaacatc tccgtgaaga ggaaaacaag ttgaagtggg aagggacctt tgccgaatac
       61 ctggacattt taagggaacg taaagaggtt gcgcaaacgg cacattcaag agtgtatgaa
      121 atgattaaaa gccatggaga aacgaaggaa aacggctata caaaattccg cttttttgaa
      181 aatgaaattt atggtctcga agatacgatc gagcggctcg tgaacgaata tttccatcca
      241 gcagcgaaac ggctggatgt gagaaagagg atcctattgc tgatgggtcc cgtcagtggc
      301 ggaaaatcaa ccattgtcac attattgaag cgcgggttgg agcaatattc gaagacggat
      361 gaaggtgcgg tctacgccat taaagggtgc ccgatgcacg aagatcccct tcatctgatt
      421 ccggattatt tacgtgacga tttcttccat gaatttggca tccggattga aggaagctta
      481 tccccattga acacgatgcg tcttgaaaag gaatacgacg gccggattga agatgtgctg
      541 atcgaaagga tcttcttttc ggaagataag cgggtcggca tcggaacgtt cacaccttcc
      601 gatccgaaat cccaggacat tgcggatttg acagggagta tcgacttttc gacaattgcc
      661 gaatatggct cggaatcgga cccgcgcgcg taccgttttg acggtgagct gaacaaggca
      721 aatcgcggat tgatggagtt ccaggaaatg ttgaagcttg atgagaaatt cctgtggcat
      781 ctactatcgt tgacacaaga agggaacttc aaagcaggca gatttgcgtt gatcagtgca
      841 gacgaattga tcgtcgccca tacgaatgaa actgaatatc gggctttcat ctccaataaa
      901 aagaatgaag ccctccattc cagaatcatt gtcatgccga tcccttacaa cttgaaagtg
      961 agccaggaag agaaaatcta tgaaaagatg atccgcgaaa gcgacatggc ccatgtgcat
     1021 atcgctccac atgccctaag agtcgccgcc atcttctccg tgctgacacg cttgaagatc
     1081 tcgaagaaac aaggaatcga tattgtgaag aaaatgcggt tgtatgacgg cgaaaatgtg
     1141 gaaggcttca atacggcaga tttggaagag ttgaaaaagg aatttccgaa tgaaggcatg
     1201 gacggcatcg atccaagata tgtcatcaac cggatatcat cagccatcat ccgcaaagaa
     1261 gtggatgcca tcaatgcatt ggacgtactt cgatccttga aggaaggcct ggatcagcat
     1321 gcgtccattt caaaggacga caaagaggag tatctcaatt atatatcgat cgcgcgcaag
     1381 gaattcgatg aaattgcgaa gaaggaagtg cagaaggcat tcgtctattc gtatgaagag
     1441 tctgcccgca cgttgatgga taactatttg gataatgtcg aggcattttg caataaacat
     1501 aaaatgaaag atccccttac tggcgaggag atgaatcccg atgaaaagct catgcgctcc
     1561 attgaagagc agatcgggat atcggaaaat gcaaaaaggg cgttccgtga agaaatcctc
     1621 atccggattt ccgcatatgc aaggaaaggc aagcgttttg attataaatc tcatgagcgg
     1681 ctgcgcgagg cgattcagaa gaaattgttc gccgacttga aggacgttgt caaaatcacc
     1741 acgtcatcgg caacgcctga cgaaacgcat ttgaagaaaa tcaatgaagt cgttgcaagg
     1801 ctcattgatg agtatggcta caattcaatt tcggcaaatg aattgttgcg gtacgtcggc
     1861 agtctcttga atcgataagc aaacccaccc atatcaatat cccgccagtc gttgcatata
     1921 atacaaaaat gagtggactg gagtaggtga atagaaatgc acgaagagca gagcgaatct
     1981 tttgtcgtct cccaggaaaa ctggtcgctt caccgaaaag gatatcagga tcagaaacgt
     2041 catttggata aagtgaagaa agcaatccac aataacctgc cagagcttat cagtgaagaa
     2101 agcattgtca tgtcggatgg cagggacgtc ataaaaatcc cgatacgatc tttggatgaa
     2161 tacaaaatcc ggtataacca caacaaatcg aaacatgtcg gccaaggcag cggggatagt
     2221 caagtggggg acgttgtcgc aagtgacgga agtcaaagcc caggaaaagg taagcaggca
     2281 ggcgatcagc ctggccagga ttattatgaa gcggaagtgt caatcgccga aatcgaagag
     2341 acgttattca aagagcttga attgccgaat cttgaaaaaa gggaacaggc ggagatcact
     2401 gaagaggaca ttcaattcaa tgatatccgg aagaaagggc tcatcggcaa tattgataaa
     2461 aagcggacga tcatcgcagc attcaaacgg aatgcaatgg aaggccatgc cgatatcatg
     2521 ccgatctata atgatgattt gcgtttcaag acatgggatg aagtcgtcaa gccgcaatcg
     2581 aaagcggtcg tgctcatgat gatggatacg agtgcttcga tgggcacttt tgaaaaatat
     2641 atggccagaa gcttctactt ctggatgacg aagtttttgc ggacgaaata cgaaaaagtg
     2701 gatatcgaat tcatcgccca tcatacaatt gcgaaagtcg tcacggaagt cgacttcttc
     2761 tcgaaagggg agagcggagg cacgatttgt tcgaccgcct atgaaaaagc gctcgatctc
     2821 attgcacaga aatataatcc gtcacgctat aatatatatc ctttccattt ctctgatggg
     2881 gaaaatatgt cctcggataa cgggaaatgc atgtcgttag tggaggagct catggaagta
     2941 tcgaatatgt tcggctatgg ggaagtgaat gcgcatagcc gctattcgac gctgatgaat
     3001 acgtataaaa aaatcgagga cccgaaattc cgtcat
//
LOCUS       DASULI010000100         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_235842, whole
            genome shotgun sequence.
ACCESSION   DASULI010000100 DASULI010000000
VERSION     DASULI010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235842"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /gene="cyoE"
                     /locus_tag="VFX34_02195"
     CDS             complement(<1..789)
                     /gene="cyoE"
                     /locus_tag="VFX34_02195"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX5563746.1"
                     /translation="MSNGRAISSSVEPDIGSTSILKDFLALIKIGIVNSNLITTFTGL
                     FLAFQFTGKSFISNLDLLLYGLFGTGLIIAGSAALNNLIDRDIDPIMSRTKSRPTVTG
                     RFKAPAVLALAFTFIALGEVMLFSASLAAGLWGLAGVFSYVVLYSMWSKRKHVSNTVV
                     GSISGAIPPLIGWAAVEPSLGMGAWALFLIMFAWQPPHFYALAMRRTEEYREAGIPML
                     PVVKGFERTKKSILGWVLLLFPLPFLLSELGIGFIALATALNIGW"
     gene            1127..2038
                     /locus_tag="VFX34_02200"
     CDS             1127..2038
                     /locus_tag="VFX34_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HEX5563747.1"
                     /translation="MRYNRYLKWFGVASTIGMLLILLGGALVTKTDSGMGCGRHWPGC
                     NGQLIPDEITPEVLIEFSHRLVTGSVGILIVVLAVWSWKAIGHKRETKLLSFLALFFL
                     ILQALIGAAQVLWGQGDFILALHFGISLISFASVFLLTLLVFEVDQKFDADKLAIGRK
                     LRFHTIGVTIYSYIVVYTGALVRHTESELSCTDWPMCKNGDFSLPANFWEWVQMSHRT
                     AAALIFIWLGIIAIHAIRNYKDQRVIYWGWITAFILVTLQVLAGGAVILTRLNLYLAL
                     AHSLFVILLFGLLTYMVLLVSRSRQQK"
     gene            complement(2084..>3049)
                     /locus_tag="VFX34_02205"
     CDS             complement(2084..>3049)
                     /locus_tag="VFX34_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5563748.1"
                     /translation="SEVYVHEMPGGQYSNLQQQAKAVGLGERWEEVKEMYSRVNMLFG
                     DVVKVTPSSKVVGDMALFMVQNDLNEETVITRGQSIDFPESVIEFFGGYIGQPYGGFP
                     KELQQVILKGRTPITVRPGELMDDVDFEALQVKTEELLGHPSTMKDALAYALYPKVFE
                     EYSAMKKEFGNISVINTPEFLYGMRLGQEIEVEIERGKTLIIKLVSIGEPQTDGTRII
                     YFELNGQPREVIIQDINVESDVARKQKADPSNDSHIAATMPGTVLNVAVSKGAKVRRG
                     EHLLITEAMKMETTIQAPFDGIVKDIHVSPGDAISTGDLLLELEH"
BASE COUNT          751 a          728 c          695 g          875 t
ORIGIN      
        1 ccaaccgata ttcagagccg tcgccagcgc aatgaatcca attccgagtt ccgaaagaag
       61 gaatggcaat gggaacaata ggagcaccca gcctaaaatc gacttcttcg tcctttcaaa
      121 ccctttcacg actggaagca tcggaatgcc ggcttcccgg tattcttcag tccttcgcat
      181 cgccaatgca tagaaatggg gaggctgcca tgcaaacatg atgaggaaca gcgcccatgc
      241 acccatacca agagatggtt cgaccgccgc ccaaccgatc aatggcggta tggccccaga
      301 gatgctgcct acaaccgtat tgctgacatg ctttcttttc gaccacattg aatagagtac
      361 aacatagctg aacacacctg caagtcccca tagtcctgcc gccagtgacg ccgaaaataa
      421 catcacttcg cctaaggcaa tgaaagtgaa cgccagtgcc aagacagccg gtgctttaaa
      481 tctgccagta accgttggac gtgactttgt tctggacatg atcggatcga tatcccgatc
      541 aataagattg ttcaaagccg ccgagcctgc gatgatcaat cccgtaccga aaagtccata
      601 cagaagcaag tccaagttgc tgatgaaact tttgcccgtg aattggaatg ctagaaaaag
      661 accggtgaat gttgtaatca ggtttgaatt cacaatgccg attttaataa gtgctagaaa
      721 atccttcaat atggatgtcg atccgatatc cggttcaacg gatgaagaaa tcgctcgacc
      781 gtttgacata tgtaccctcc tttcaaggat tgtcggcaag gaccgctata cttcactata
      841 tcgaattccg gcaataattt ctatatataa cccgaatttc gcctatatgt aacccgatat
      901 gtgaagtata tatgtcttta tgaattgttt cgccttatct atagtaaatc atttccccat
      961 gccttttgtg aagttaaagg gtctatttta tgaacaattt gtgaaaaagc ggtacccaaa
     1021 ctcaaatttg gccaatatgc gttgtttttt cgcttttttt tcgctatcat caacaaacgg
     1081 ggcctttttt cataatggtc atcatctctt aaagaaggtg tcttgtttgc gatataatag
     1141 atatttgaaa tggttcggag tcgcctccac aatcggcatg ctgctgatcc tgttgggcgg
     1201 ggctcttgtt acgaagacgg atagcgggat gggatgcgga aggcattggc ccggctgtaa
     1261 cggtcagttg atcccggatg aaatcacccc tgaagtcctt attgaatttt cgcatcgcct
     1321 cgttactgga tcggttggca ttttaatcgt tgtcctggca gtatggtcat ggaaagcgat
     1381 cggacataaa agggagacaa agcttctttc ctttttggca ctattcttct tgattttgca
     1441 ggcattgatc ggagcagcgc aagtcctttg gggacaaggc gacttcatcc tagcacttca
     1501 tttcggcatt tcgctcattt cctttgcatc cgtgtttctt ctcacattac tggtctttga
     1561 agtcgatcag aaatttgatg cggataagct ggcaatcggt aggaagctta gattccacac
     1621 gatcggcgta accatctatt cctatattgt cgtgtacacg ggagcacttg tgcgtcatac
     1681 tgaatccgaa ttgagctgta cggattggcc gatgtgcaag aacggggatt tctccctgcc
     1741 cgctaacttc tgggaatggg tgcagatgag tcatcggacg gcagccgctt tgatattcat
     1801 ttggcttggg attattgcga ttcacgcaat cagaaattat aaagaccaac gggtcatcta
     1861 ttggggatgg attaccgctt tcattcttgt gactttgcag gtgttagcag gcggagctgt
     1921 cattcttacg cgtttgaatc tctatttggc tttggctcac tcgttgttcg tgattctcct
     1981 gttcggactt ttaacttata tggtgcttct agtatcgagg agcaggcagc aaaaataacg
     2041 gaagcacccg cttggatatc caggcgggtg cttttttcaa tcattaatgc tctagctcta
     2101 gtagtagatc tcctgttgag atggcatcac ctggggagac gtggatgtct ttgacgattc
     2161 catcgaaagg tgcctggatc gtcgtttcca tcttcattgc ttccgtaata agcaagtgtt
     2221 cccctcttct gactttcgca cctttggaga ccgcgacatt caatacggta ccaggcattg
     2281 tcgctgcaat atgggaatcg ttggacgggt ccgctttttg cttgcgggct acatccgatt
     2341 ccacattgat atcttggatg attacttccc taggctggcc gttcagctcg aagtagatga
     2401 tacgcgtccc atctgtttgc ggctcaccga tggagacgag cttgatgatg agggtcttcc
     2461 ctctttcgat ctccacttcg atttcctgac ccaacctcat tccgtaaagg aattctggtg
     2521 tgttgatgac ggaaatgttt ccgaattcct ttttcatggc ggaatattct tcgaacactt
     2581 tcggatagag cgcataggct agcgcatctt tcatcgtgga cggatgtccg agaagttctt
     2641 ccgttttaac ctgaagtgct tcaaagtcca catcatccat caattctccc ggacggacag
     2701 taatcggtgt acgcccttta aggatgactt gctgcaattc tttcgggaac cctccgtatg
     2761 gctggccgat atagcctccg aaaaactcga tgacggattc agggaagtca atcgattgcc
     2821 cgcgtgtaat gacggtttcc tcattcaaat cattttggac catgaataac gccatatccc
     2881 ctacaacttt tgaagaagga gtcactttaa caacatcacc gaaaagcata ttgacacgcg
     2941 aatacatttc cttgacttct tcccatcttt caccgagacc gacagctttc gcctgctgct
     3001 gcaagttgga gtattggcca cccggcatct catgtacata cacttcgga
//
LOCUS       DASULI010000101         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_304326, whole
            genome shotgun sequence.
ACCESSION   DASULI010000101 DASULI010000000
VERSION     DASULI010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304326"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /gene="dapB"
                     /locus_tag="VFX34_02210"
     CDS             <1..659
                     /gene="dapB"
                     /locus_tag="VFX34_02210"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX5563749.1"
                     /translation="NEEKQGIPIYTSLSILCAEHQPAVLLDVTDPDAVFNNVKDALAL
                     GVHPVVGTSGLTKQELESIFKMVESTQVGGIIAPNFSIGAVLMMKFAAQAARYLGDVE
                     ILEMHHDRKLDAPSGTAVKTADMIMANRPAHIQGHPEEQVHLEGARGADVEGMKIHSI
                     RLPGLLAHQQVLLGGEGELLTIRHDSFDRKSFMPGIMMAIREVIDRKDLVYGLENIID
                     "
     gene            684..1817
                     /gene="bshA"
                     /locus_tag="VFX34_02215"
     CDS             684..1817
                     /gene="bshA"
                     /locus_tag="VFX34_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEX5563750.1"
                     /translation="MKKLKVGIICYPTLGGSGVVATELGLKMAERGHEMHFITSSRPF
                     RITEGHPNIHFHEVKIDGYAVFKYPPYDIALANRIARVIEEEELDLLHVHYAVPHAIS
                     AALAKDMANSDIGVITTLHGTDVTILGHDPALKNTVRYGIEKSDLTTAVSESLRQDTL
                     SLIEPQKEILTIYNFIDEEKYRPVDPGSLKSDLGIREDEKVLIHISNFRSVKRISDII
                     DSFKLLRKEIDSKLVLVGTGPEKLEMMEKAKKEGLEEEIIFTGKRDDLPELLAISDVM
                     FLLSEKEAFGLVLLEGFACGVPAIATNIGGIPEVIEDGSNGFLVELGDVETAAKKAKL
                     LLEDPVMHKRFRESALHTVSNKFNSSSIVLRYEELYYRVAGMQ"
     gene            1814..>2505
                     /locus_tag="VFX34_02220"
     CDS             1814..>2505
                     /locus_tag="VFX34_02220"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX5563751.1"
                     /translation="MTIHFGTESSRKVVRELEEAGYEAVFVGGAVRDHLLGKEPSDFD
                     IATSAMPEEVKRVFKHTIDVGIAHGTVMVMMDNEPIEVTTYRTESTYTDHRRPDEVNF
                     VRSLKEDLQRRDFTMNALALTLTGELIDPFGGMEDLRACKIRAVGNASDRFHEDALRM
                     IRAIRFSSVLGFDIEEQTLQAIEENAASIRHVSIERIKAEMDKLWIGANPGKALASIV
                     DTRLSTHLPLFP"
BASE COUNT          701 a          521 c          668 g          615 t
ORIGIN      
        1 taaatgaaga gaaacaagga atcccgatct acacatcgct atccatactt tgtgcggaac
       61 atcaaccggc cgtgctgttg gatgtaaccg atccggatgc cgtattcaat aatgtaaaag
      121 atgcattggc acttggcgtc catcccgttg tgggcacgtc cggtttgacg aagcaggaat
      181 tggaatccat tttcaaaatg gtggaatcga ctcaagtcgg agggattatc gctccaaact
      241 tttccatcgg cgctgtgctc atgatgaaat ttgccgcaca ggccgcccgt tacttagggg
      301 atgtggagat tcttgaaatg catcacgatc ggaaattgga tgcgccttcc ggaacggctg
      361 ttaaaacggc agatatgata atggcaaaca ggccggcgca tattcaaggt catccggagg
      421 agcaagtcca tttggaaggc gcgcgcggcg cagatgtgga aggtatgaaa atccatagta
      481 ttcgtttgcc tggtctgctt gcacatcagc aagtccttct tggaggagaa ggggaattat
      541 tgacgattcg acatgattca tttgatagga aaagctttat gcccgggatt atgatggcca
      601 tacgggaagt gatcgaccga aaggaccttg tgtacggatt ggaaaacatt attgattaac
      661 gttgtgcgga agaggtgcag tttatgaaga aattgaaagt cggtatcatt tgctatccta
      721 cattaggggg atccggggtt gttgcaacgg aactcggact aaaaatggcg gaaagagggc
      781 atgaaatgca tttcatcact tccagtcggc ctttccgtat tacagagggc caccccaata
      841 ttcatttcca cgaagtgaaa atcgacggtt atgccgtttt caaataccct ccgtacgata
      901 tcgctttagc gaaccggatt gctcgggtga tcgaggagga agagctggat ctattgcacg
      961 tccattacgc cgtcccgcat gccatttccg ctgcactggc aaaggatatg gcgaactcgg
     1021 atattggtgt cattacgaca ttgcatggga cggacgtgac cattcttggc catgaccctg
     1081 ctttgaaaaa cacagtccgt tatggcatcg aaaagtccga tttgacgaca gctgtttccg
     1141 aatcccttcg gcaggataca ctttcattaa ttgaaccgca aaaagaaata ctgacaatct
     1201 ataacttcat cgacgaagag aaataccgac ctgtcgatcc tggttccttg aagagcgatt
     1261 tgggaatccg ggaggatgaa aaagtcctca ttcatatttc gaatttccga agtgtcaaaa
     1321 ggatttcgga tattattgac agcttcaaac ttctccgaaa ggaaatcgat tcgaaattag
     1381 tgttggtagg tacgggtcct gaaaaacttg aaatgatgga aaaagcaaaa aaagaaggtt
     1441 tagaagagga gattatcttt acaggaaagc gagatgactt gcctgaacta ttagcaataa
     1501 gcgatgtcat gttcctatta tctgaaaaag aagcgttcgg ccttgtcctt ctcgaaggct
     1561 tcgcttgcgg cgtgcctgca atcgcgacaa acatcggcgg gattcccgaa gtgatcgagg
     1621 acggctcgaa cggtttcctc gtagagttgg gtgatgtgga gacggctgcg aaaaaagcga
     1681 agctgcttct ggaagacccg gtcatgcata agcgcttccg ggaaagtgca ttgcatacag
     1741 tcagcaataa attcaattca tcctcaatcg ttcttcgata cgaagaactg tattaccgag
     1801 tggcgggtat gcaatgacaa tacatttcgg aacagagtcg agccggaaag tcgtccgtga
     1861 attggaggaa gcgggttatg aagccgtctt tgtaggaggg gcggttcggg accatttgct
     1921 tggcaaagag ccatcggatt tcgatatcgc cacgtcagca atgccggaag aagtgaagcg
     1981 agtcttcaaa cacacgatcg atgtaggcat cgcacatggg acggtcatgg tgatgatgga
     2041 caatgaaccg attgaagtga ctacgtaccg taccgaaagt acatatacgg accataggag
     2101 acctgatgaa gtgaactttg tcaggtcgtt gaaagaggac ttgcaacgaa gggatttcac
     2161 aatgaacgct ttagccttga cactgaccgg tgaactgatc gacccattcg gggggatgga
     2221 agatctgagg gcttgtaaaa tccgggcagt cgggaatgca tcagataggt ttcatgaaga
     2281 tgcgctgcga atgatccgtg caatccgatt ttcgtcggtg ctaggatttg atatcgaaga
     2341 acagacctta caggctattg aagaaaatgc tgcaagtatc aggcatgtct ccattgaacg
     2401 gatcaaggcg gaaatggata agttatggat tggcgcgaat ccaggcaaag cgcttgcatc
     2461 gatagtggat acccgccttt ctacgcattt gccgcttttt ccgaa
//
LOCUS       DASULI010000102         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_570779, whole
            genome shotgun sequence.
ACCESSION   DASULI010000102 DASULI010000000
VERSION     DASULI010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570779"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFX34_02225"
     CDS             <1..331
                     /locus_tag="VFX34_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEX5563752.1"
                     /translation="ARKLITGEELVVREVRSGNARLVIVSEDASKNTLKKVNDKCSFY
                     NVEKHVFGSREALGHAIGKESRVVLALTDAGFAGKLSGLLNEYNRGWANDEDTCTRIR
                     ETSESDK"
     gene            279..2735
                     /gene="infB"
                     /locus_tag="VFX34_02230"
     CDS             279..2735
                     /gene="infB"
                     /locus_tag="VFX34_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5563753.1"
                     /translation="MTKIRVHEYAKQVNRTSKEVIEELGKLNVNVTNHMSMLDKEATS
                     KLDQRFGKGGSTPSDKPRSGQNDRKPSSNASRPAGNAQDNKRQGGQRQGQGGNRPAAQ
                     GGQRQGQGAQGGNRPAQGGQRQGQGSQGGNRPAQGGQRQGQGAQGGNRPAQGGQRQGQ
                     GAQGSNRPAQGGQRQGQGVQGGNRPAQGGQRQGQGPQGGNRPAGRGGRPPARGINQGR
                     RRHRPAPMPKVEKPLPEKITFYESLTVGELANKLGREPSEIIKKLFMLGVMATINQEL
                     DKDAIELICAEYDVEVEEEIRIDVTDLEIYFEESEEPEGELTERPPVVTIMGHVDHGK
                     TTLLDSIRDTKVTQGEAGGITQHIGAYQIEAEGKKITFLDTPGHAAFTTMRARGAKVT
                     DITILVVAADDGVMPQTIEAINHAKAAEVPIIVAVNKMDKPTANPDRVMQELTEHGLL
                     SEAWGGDTIFVPISALKGEGIDQLLEMVLLVAEVAELKASKNIRARGTVIEAQLDRGR
                     GSVATLLVQDGTLRVGDPIVVGNTFGKVRAMINDLGRRVKEAGPSTPVEITGLSDVPQ
                     AGDRFVVFEDEKTARQIGESRAGDALQEQRGEKTRVTLDNLFDQMKQGEMKELNLIVK
                     ADVQGTVEAMAASLMKIEVEGVNVRIIHTGAGAINESDISLAAASNAIVIGFNVRPDV
                     NAKRAAEEEGVDIRLHRVIYKVIEEIESAMKGLLDPEYEEKVIGQAEVRETFKVSKIG
                     TIAGSYVTEGKITRDAGVRVLRDNIVIFEGELDTLKRFKDDVKEVARGYECGITVKNF
                     NDIKEGDIFEAFIMEEIKRT"
     gene            2732..3010
                     /locus_tag="VFX34_02235"
     CDS             2732..3010
                     /locus_tag="VFX34_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX5563754.1"
                     /translation="MIVYAECTFFIPDVHSLKGKRSVLKRMTDRVKNEFNVSIAEIDH
                     QDLWQRTTIALVSVAASKDAAEGETRRAIRFLESNPEWEMTEMILDYY"
     gene            3033..3380
                     /gene="rbfA"
                     /locus_tag="VFX34_02240"
     CDS             3033..3380
                     /gene="rbfA"
                     /locus_tag="VFX34_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5563755.1"
                     /translation="MSMRANRVAEQMKKELGGIIGQKLKDPRIGFVTVTDVEVTGDLQ
                     QATIFISVLGNESEKEATLAGLTKAKGFIRTEIGQRIRLRKTPEIEFAFDDSVEYGNR
                     IESLLRQVNTEEE"
BASE COUNT         1052 a          780 c          967 g          711 t
ORIGIN      
        1 agcccggaaa ctgattaccg gcgaagagtt ggtcgtcagg gaagttcgtt cgggcaatgc
       61 gcgtttagtt atcgtttctg aagatgcttc aaaaaacacg cttaaaaaag tgaatgataa
      121 atgcagtttt tacaacgttg agaagcatgt attcgggagc cgggaagctc tcggccatgc
      181 aatcggaaaa gagtcgcggg tcgtactcgc gttaacggat gccggctttg ccggaaaatt
      241 atccgggctc ctcaacgaat ataaccgggg gtgggctaat gacgaagata cgtgtacacg
      301 aatacgcgaa acaagtgaat cggacaagta aagaagttat cgaagagctt ggaaaactaa
      361 atgtgaatgt aacgaaccac atgtcaatgc ttgataaaga ggcgacatca aaattggatc
      421 aacgttttgg taaaggcggt tctacgccgt ccgataagcc tagaagcgga caaaatgacc
      481 ggaagccgtc ttcgaatgct tctcgtcccg caggcaatgc acaggacaac aagagacaag
      541 gcggccagcg tcaagggcaa ggcggcaatc gtccagcagc acaaggaggc caacgtcaag
      601 ggcaaggcgc acaaggcggc aaccgtccgg cacaaggcgg tcaacgccaa ggccaaggat
      661 cccagggcgg caaccgacca gcacaaggcg gtcagcgcca aggacaaggc gcacaaggcg
      721 gcaaccgacc agcacaaggc ggtcagcgtc aaggacaagg cgcgcaaggc agcaaccgac
      781 cagctcaagg cggccaacgt caagggcaag gtgtgcaagg cggcaaccgt ccggcacaag
      841 gcggtcaacg tcaagggcaa ggaccacaag gcggcaatcg cccagctgga cgtggtggaa
      901 gaccaccggc acgtggcata aaccaagggc gcagaagaca tcgtccagct ccaatgccta
      961 aagtggagaa gccgttgccg gaaaaaatca ctttctatga atcattgaca gtcggtgaac
     1021 ttgcgaacaa actgggcaga gaaccgtctg aaatcatcaa aaagctattc atgcttggag
     1081 tcatggcaac aattaaccaa gagttggata aagatgcaat cgagctgatt tgtgcggaat
     1141 acgacgtgga agtggaagaa gaaatccgca tcgatgtcac tgatctggaa atttactttg
     1201 aggaatcgga agagcccgaa ggtgaactga cagaacgtcc tccagtcgta acaatcatgg
     1261 gacacgttga ccacggtaaa acgacgcttc tcgattcgat ccgtgacacg aaagtgactc
     1321 agggcgaagc gggcggcatc actcagcata tcggtgctta tcaaatcgaa gctgaaggca
     1381 agaaaatcac gttcctcgat actcctggac acgcggcgtt tacaacaatg cgtgcgcgtg
     1441 gagcgaaagt gactgatatt acaattctcg tcgttgcagc ggatgacggt gtcatgcctc
     1501 aaacaattga agcgatcaac catgcgaaag ccgctgaagt gccgatcatc gtcgcggtga
     1561 ataaaatgga taagccgact gcaaacccgg accgagtcat gcaggagttg acagagcatg
     1621 gcctattgtc ggaagcgtgg ggcggagata cgatctttgt accgatttcc gcgttaaaag
     1681 gcgaagggat cgatcaactg cttgaaatgg tcttattagt agcggaagtg gctgagttga
     1741 aggctagcaa aaacattcgc gcaagaggga ctgtcatcga agcccaattg gaccgaggcc
     1801 gtggatctgt cgcaaccctt ctcgttcaag atggtacact tcgcgtaggt gacccgatcg
     1861 tcgtcgggaa cactttcggt aaagtgcgtg cgatgatcaa cgaccttggc cgccgtgtga
     1921 aagaagcagg tccgtccacg ccggttgaaa ttaccggtct cagcgatgtg ccgcaagcgg
     1981 gtgatcgttt cgtcgtattc gaagacgaaa agacagcccg tcaaatcggt gaatcgagag
     2041 caggagacgc attgcaggag cagcgtggcg agaaaacacg cgtgacgctt gataatctat
     2101 ttgatcagat gaaacaaggg gaaatgaagg aattgaacct tatcgtcaaa gccgacgttc
     2161 aaggaactgt cgaggcgatg gccgcttccc ttatgaaaat cgaagtcgaa ggcgtcaacg
     2221 tacggatcat tcataccgga gcaggagcca tcaatgagtc ggacatttcc ctagccgcag
     2281 catccaatgc aatcgtcatc ggtttcaacg tgcgtccaga tgtcaatgct aaacgtgcag
     2341 ccgaagagga aggcgtcgat atccgattgc accgcgtcat ctataaagtg atcgaggaaa
     2401 tcgagtcggc gatgaaaggt ctattggatc ctgaatacga ggaaaaagtg atcggccaag
     2461 ccgaagtccg tgaaacattc aaagtttcga aaatcggaac gattgccgga agttatgtta
     2521 cagaaggtaa aattacccgt gacgctggcg tacgcgtcct ccgtgacaat attgtcattt
     2581 tcgaaggcga acttgacaca ttgaagcgat tcaaggatga cgtcaaagaa gttgcgagag
     2641 gttacgaatg tggaatcacc gttaaaaact tcaatgatat taaggaaggc gacatttttg
     2701 aagccttcat catggaggaa atcaaacgga catgatcgtc tatgcggaat gtacattctt
     2761 catacctgac gtccattcat taaaaggcaa acgatctgtt ttgaaaagaa tgacggatcg
     2821 agtgaaaaat gagttcaacg tttccatagc cgaaatcgat caccaagact tatggcaacg
     2881 aactacgatc gctctcgttt cagtcgctgc ctcaaaagac gcagcggagg gagaaacgag
     2941 aagagccatc cgttttttgg aatcgaatcc ggaatgggaa atgacagaaa tgatacttga
     3001 ctactattga tacggaatcg gggtgaccat ccatgtctat gcgtgcgaat cgtgttgctg
     3061 aacagatgaa gaaagaatta ggtggaatca tcggccaaaa attgaaagac cctcgcatcg
     3121 gctttgttac tgtgacggac gtcgaagtga caggcgatct tcaacaggcg accattttca
     3181 tctcggtatt aggaaatgaa tctgaaaaag aggcgactct tgccggtctt acgaaggcga
     3241 aagggtttat ccgcactgaa atcgggcaac ggatcagact caggaagaca cctgaaatcg
     3301 aatttgcatt tgatgattct gtcgaatacg gaaatcgtat tgaatcgctg cttcgtcaag
     3361 tgaatactga agaagaatga acgatgggag gaagtcggcg tccgaatgga ctttccggct
     3421 tcctcctttt tattactaaa aacaaagtga ggaggatgaa tatggacggg attcttcctt
     3481 tgtggaagca aaaagggatg acttcgcatg
//
LOCUS       DASULI010000103         6992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_218385, whole
            genome shotgun sequence.
ACCESSION   DASULI010000103 DASULI010000000
VERSION     DASULI010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218385"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /gene="aspS"
                     /locus_tag="VFX34_02245"
     CDS             <1..1227
                     /gene="aspS"
                     /locus_tag="VFX34_02245"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5563756.1"
                     /translation="RDYLVPSRVHPGEFYALPQSPQLFKQMLMVSGFDRYYQIARCFR
                     DEDLRADRQPEFTQIDMEMSFMSIEDIMELNERLMKQVMKDVKGIDIEIPFKRMPYDE
                     AMGRFGSDKPDTRFGMELQDVSEIVKDSSFKVFAGAVESGGQVKAINVKGAADNYSRK
                     DIDALGEYASRYGAKGLAWLKVDAEGLKGPIAKFFEGEAGEALASSLGAEAGDLLLFV
                     ADKKSVVADALGALRLKLGKELNLIDETKFNFLWVTEWPLFEYDEEEGRYYAAHHPFT
                     MPADVAGLESDPSGVKAQAYDLVLNGFELGGGSLRIYQREVQEKMFKALGFTEEQARE
                     QFGFLLDAFEYGTPPHGGIAFGLDRIVMLLSGSTNLRDTIAFPKTASASDLLTEAPSS
                     VDGSQLTELGISIQSK"
     gene            1322..1503
                     /gene="ssrS"
                     /locus_tag="VFX34_02250"
     ncRNA           1322..1503
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFX34_02250"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1601..2368
                     /locus_tag="VFX34_02255"
     CDS             1601..2368
                     /locus_tag="VFX34_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX5563757.1"
                     /translation="MLHQFSRNELAIGKEGVDLMRQTTVAILGVGGVGSFAAEACARS
                     GVGRIILIDKDTVDITNVNRQLVATLSTIGKSKVEVMKERIADVNKDCEVIGLHMFYT
                     EETYEEFFSYEPDYVIDASDTISYKIHLIKECVARGIKIISSMGAANKLDPTRFQIAD
                     ISKTHTDPIAKVIRLRLRKEGIKKGVPVVFSDESPIVVREDVVDTVGKPDAQIRKAKM
                     PPASNSYVPSVAGLVCASWVLNDIVKDIHIERVKDKK"
     gene            2505..3410
                     /locus_tag="VFX34_02260"
     CDS             2505..3410
                     /locus_tag="VFX34_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEX5563758.1"
                     /translation="MQKQDGILLESGTNELEIVEFQMGQNRYGINVIKVKEIILPTPI
                     TIIPHAHPSIEGIIQLRGAVLPVINMEKVMGLPETIGKNEGKYIVAAFNRQEVVFHVH
                     EVAQIHRVSWDQIEKPADLYSGDASQVIGVIKRESDMLLLLDFEKIVVDINPDSGIQV
                     EQVRKLGVRERSNKKILVAEDSPLLRKLLSDTLTEAGYGNIEFFENGKDALTFLESLA
                     SEGKKVSEEIQLVITDIEMPQMDGHHFTKRIRSNKALEMLPIIIFSSLITDELKHKGI
                     SVGANDQVSKPEIAELVLKMDKHIL"
     gene            complement(3459..4745)
                     /locus_tag="VFX34_02265"
     CDS             complement(3459..4745)
                     /locus_tag="VFX34_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5563759.1"
                     /translation="MHNEPLAFRMRPKNIDEVAGQQHIIGKDTALYRMISKGHVPSML
                     LYGEPGIGKTSLAYAIAGTSHLPFIPLNATVSGKKDVEQVVAEARITGKVILFLDEIH
                     RFNKLQQDTLLPHVEKGSIVLIGATTENPFHDVNPAIRSRCGEILQLNRLEPDDLVEL
                     IERALHDEDRGLGKMKIEMTDSQIRKIAEGVNGDARKALTLLESIISASDEEEGNVIV
                     EDWLIDNLIGRIGLVGDKKGSHHYNLLSALQKSVRGSDVNAAIFYLANLLEIGDLIAV
                     SRRLLVMAYEDIGLAAPEVGPHVLAATEAAVRLGMPEARIPLANAVIEMCLASKSNSA
                     YKAVDAAVKAINDGKTGDIPLHLRDTHYAGAAELGHEGYRYPHDTPIGTFGGWVNQQY
                     LPDKINQAEFYEPVLAGEEKRLAGIYERLKDFKKEK"
     gene            4902..5321
                     /locus_tag="VFX34_02270"
     CDS             4902..5321
                     /locus_tag="VFX34_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5563760.1"
                     /translation="MRISTKGRYGLTVVVELGKKYGEGPLPLRKIAEEHNLSEAYLEQ
                     LIPALRNSGIVKSVRGAYGGYKLAKPPGEITAGDVIRLLEGPIQVVEGIDDDKVPQQS
                     LWNRIGDAIREVLDTTTIKDLVESDESTDLDGYMFYI"
     gene            5347..6489
                     /locus_tag="VFX34_02275"
     CDS             5347..6489
                     /locus_tag="VFX34_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5563761.1"
                     /translation="MQTIYLDHAATTPIHPEVSTVYVKAMDTVFGNPSSIHSFGRSSR
                     KLLDDARKAIAEAIHAEPNEIIFTSGGTEADNVAIFGTAAAMKEKGNHIITTAIEHHA
                     VLNSCKELEKNGFHVTYLPVDENGRVSAEQVAEALTDQTVLVTVMYGNNEVGTIQPIK
                     EIGEIVKDHQAVFHTDAVQAFGIMDLDVNELQVDLLSASAHKLNGPKGVGFLYQRKGL
                     QTTPLQFGGEQERKRRAGTENVPAIAGFAEAIKIARNEMDENHRKYDRYKTILTDVFI
                     QEGIVFEVNADKVERLPHVVNISFPGTEIESLLVNLDMAGVAVSSGSACTAGSLEPSH
                     VLSAMWGSDSAKLRNSVRFSFGLGLDEDAVREAAMKTAQIIKRLVK"
     gene            6510..>6992
                     /locus_tag="VFX34_02280"
     CDS             6510..>6992
                     /locus_tag="VFX34_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX5563762.1"
                     /translation="MRTANKPISETRVVIGMSGGVDSSVAALLLKEQGYDVIGIFMKN
                     WDDTDEFGVCTATEDYEDVISVCNEIGIPYYAVNFEQQYWDKVFTYFLDEYKAGRTPN
                     PDVMCNKEIKFKAFLDHAMSLGADFLATGHYAQVVETDQGVAMLRGKDENKDQTYFLN
                     Q"
BASE COUNT         1990 a         1546 c         1697 g         1759 t
ORIGIN      
        1 agggactacc ttgtgccgag ccgggtgcat cctggagaat tttacgctct gccgcagtct
       61 cctcaacttt tcaaacaaat gctcatggtg tcaggtttcg acagatacta tcaaatcgct
      121 cgctgcttcc gtgatgaaga cctacgcgca gaccgtcaac ctgaattcac tcaaatcgat
      181 atggaaatga gcttcatgtc catcgaagac attatggaac tgaacgagcg cctaatgaag
      241 caagtgatga aggatgtcaa aggaattgac attgaaattc cgttcaagcg tatgccatat
      301 gatgaagcaa tgggacgttt cggttcagat aagccggata cccgtttcgg catggagctg
      361 caggatgtgt ccgaaatcgt gaaagactct tccttcaaag tatttgcagg tgctgttgaa
      421 tccggcggac aagtgaaagc gatcaatgtg aaaggcgcag cggataatta ttcccggaaa
      481 gatatcgatg cgcttggcga atacgcttca cgttatggag caaaagggct tgcctggttg
      541 aaagttgacg cggaaggctt aaaagggccg attgccaaat tctttgaagg tgaagcggga
      601 gaggctttag caagttcact tggcgcagaa gctggcgacc ttcttttatt tgtagcggac
      661 aagaaatcgg ttgtcgcgga tgcactggga gcactccgat tgaaactagg aaaagagttg
      721 aatctcatcg atgaaacaaa attcaatttc ctatgggtta cagaatggcc attattcgaa
      781 tatgacgaag aagaagggcg gtattatgca gcccatcacc cattcacgat gccggctgat
      841 gtggctggtc ttgagtcgga tccgagcggt gtcaaggcgc aagcgtatga cctcgtattg
      901 aatggttttg agctgggcgg aggatcattg cggatctatc aacgtgaggt ccaagagaaa
      961 atgttcaagg cacttggctt cacggaagaa caagcccgtg agcagttcgg attcctgctt
     1021 gatgcatttg aatatggcac gcctcctcac gggggaattg cattcggatt ggaccggatc
     1081 gtcatgttat tatccggttc gacaaatctc cgcgatacca tcgcatttcc gaagaccgca
     1141 agtgccagcg acctgttgac agaagcacct agcagtgtag atggctcaca acttaccgaa
     1201 ttaggaattt ccatccagtc gaaatgagtg gaacggacaa gctcctgaaa gaaaattgta
     1261 ataaattgcg gatgtgaaat aattgaacgc aattcttaag tgtggtagga tacaattaat
     1321 acgaatcctg aagtgttcgt ttcttgcaaa tcagttttga ccgaacatat ttatcgtagg
     1381 gagctgtcat tctgttttgg atggaatgcc ctcgtcaggg acttcagaaa agacggatgg
     1441 agcacccacc tgctgagtgc gggttcaaaa cgatactaca aagacggcac atttgggatt
     1501 cgttcctatt acttcactaa catttagtag actatattcc cctctggcgt tcttgcggag
     1561 gggttttgtt ttgagtcata tatgaaaggg cggaatcctt atgttacacc aattttcccg
     1621 gaatgagctg gccatcggta aggaaggcgt cgatttgatg cgtcaaacga ctgtcgctat
     1681 actaggcgtc ggaggtgtcg gttcatttgc agccgaagcc tgcgcccgaa gcggtgtcgg
     1741 ccgaatcatc ctgatcgata aagacacagt cgatattaca aacgtcaatc gccagctcgt
     1801 tgcaacattg tcgacgatcg gcaagtccaa ggtggaagta atgaaagaac gcattgcgga
     1861 cgtgaacaaa gattgcgaag tgatcggtct tcacatgttc tatacggaag agacatacga
     1921 agagtttttc agctatgaac cggattatgt aatcgatgca tcagatacaa tcagttataa
     1981 aatccatttg attaaagaat gtgttgctag aggcatcaaa atcatctcca gcatgggtgc
     2041 tgcgaacaaa cttgatccaa cccgctttca aatagcggac atttccaaaa cgcatacaga
     2101 tccgattgca aaagtgatca gactgcgatt gagaaaagaa ggaatcaaaa aaggcgttcc
     2161 tgtcgtattt tccgatgaaa gcccaattgt cgtcagggaa gatgttgtag atacagtcgg
     2221 taaacccgat gcacaaatcc gaaaagcgaa aatgccgccc gcatcaaatt cctacgtccc
     2281 atccgtcgca ggcctcgttt gcgcaagctg ggtactcaac gacatcgtaa aagacatcca
     2341 catcgaacga gtaaaggaca agaagtaata aaaagctgtt ttcctataaa tcgggaaaac
     2401 agcttttact attgttccat cattccctac ctgttacaat ctatgtaggt aaaaaatcat
     2461 aaaacttatg tattatagta taggaatcgg aagggctgaa tcaattgcaa aaacaggacg
     2521 gaattttact cgaaagcgga acgaacgagc ttgaaatcgt cgaattccaa atgggccaga
     2581 accgctacgg catcaatgtc atcaaagtca aagaaatcat cctgcctaca ccaatcacta
     2641 tcatcccgca cgctcacccg tccatcgaag ggatcatcca gctccgcgga gccgtccttc
     2701 ctgtaatcaa catggaaaaa gtgatggggc tgcctgaaac aatcggcaaa aacgaaggga
     2761 aatatatcgt cgccgccttc aacagacagg aagtcgtctt ccacgtccac gaagtggcac
     2821 aaatccatcg tgtttcgtgg gatcaaattg aaaagccggc agatctgtat tccggagatg
     2881 cctcccaagt cattggtgtc atcaaacggg aaagtgatat gcttctgctt cttgatttcg
     2941 aaaaaatcgt cgtcgatatt aacccggata gtggaattca ggttgaacag gtaagaaaac
     3001 tgggcgtaag ggaaaggtcc aataaaaaaa tcctcgttgc agaggattca ccattgctcc
     3061 gtaaattatt gagcgataca ttgacagaag caggctacgg aaatatcgag ttttttgaaa
     3121 acgggaaaga tgcactgaca tttttggaaa gtctcgcttc cgaaggcaaa aaggtatctg
     3181 aagaaattca acttgtcatc accgatattg aaatgcctca aatggatgga catcatttca
     3241 cgaaaagaat ccgttcaaat aaagcactgg aaatgcttcc gatcatcatc ttctcatcat
     3301 tgatcactga tgaactgaaa cacaaaggaa tcagtgtcgg tgcgaatgat caagtgagca
     3361 aaccggaaat tgcagaactt gtccttaaaa tggataaaca tattttataa aaaagtgagc
     3421 accgtcctta ttataaagac ggtgcttttc attcgccgtt atttttcctt tttgaaatcc
     3481 ttcagtcgtt cgtatatccc tgcaagtctt ttctcttcgc ctgcaaggac aggttcgtaa
     3541 aactccgctt gattaatttt atccggtaag tattgctggt tcacccagcc tccgaatgtt
     3601 ccgatcgggg tgtcatgagg gtagcggtac ccttcatggc cgagctcagc ggctcctgcg
     3661 taatgggtat cccgtaaatg gagagggata tctcctgttt tcccatcgtt gatcgctttc
     3721 acggcggcgt cgaccgcctt atatgccgaa ttcgatttgg aagcgaggca catctcgatg
     3781 actgcgtttg ccaatggaat gcgcgcttcc ggcataccga gacgaacagc cgcctcggta
     3841 gctgcgagca catggggacc tacttccggt gcagccaatc cgatgtcttc atacgccatg
     3901 acgagcagtc ggcgcgatac cgcaatcagg tcacctatct caagcagatt tgccaaatag
     3961 aagatagctg cattcacatc gctgccacgc actgacttct gcaacgcgga aagcagatta
     4021 taatgatgcg atcccttctt atccccgaca agcccgatcc ttccgatcaa attatcgatc
     4081 aaccagtctt caacaatgac attgccttct tcttcatcgc ttgcggaaat gatggattcc
     4141 aataatgtga gcgcctttcg tgcatctccg tttacacctt cagcaatttt ccttatctgg
     4201 ctatcagtca tctcgatttt cattttcccg agaccgcggt cttcatcatg caaggcacgt
     4261 tcgattaatt caacaagatc atcaggctcc agccggttca attgaagaat ctcgccgcat
     4321 ctcgaccgaa ttgccggatt cacatcgtgg aaaggatttt ctgtcgttgc accgatcagc
     4381 acgatcgatc ctttttcgac atggggcaat agtgtgtctt gctgcagttt attaaaacga
     4441 tggatctcat ccaagaacaa gatgacctta cctgtgatcc gggcttctgc aacaacttgc
     4501 tctacgtcct tcttgccgga tactgtcgca ttcagtggga tgaacggcaa atggctcgta
     4561 cctgcaatcg cgtatgccaa tgatgttttt ccgattcctg gctcgccgta tagcagcatg
     4621 gatggcacat gccctttgct gatcatccgg tacagcgcag tatctttccc gataatatgt
     4681 tgctgtcctg caacttcgtc tatgttttta ggtctcatcc gaaatgctaa tggttcatta
     4741 tgcaatcaaa ttcactcccc cgtacatccg ttccactatt atacacaacg cagagcagga
     4801 agtcatttga aggaagcgcc agagcagtta tgctataatg ttttgaagtg tattgatgaa
     4861 attacggcct gaattggacg agaaaaggac gttggttata tatgaggatt tctacgaaag
     4921 gccgctatgg tctgactgta gttgtggaac ttggtaaaaa atacggggaa ggccctctgc
     4981 cattgcgcaa aatcgcagaa gagcataacc tgtcggaagc gtatttggag caactcatcc
     5041 ctgcattgcg gaatagtgga attgtaaaaa gcgtccgtgg tgcatacggg ggctataaac
     5101 ttgcaaagcc gcccggtgaa atcaccgcag gggacgtgat caggttgctt gaaggtccta
     5161 tacaggttgt cgaaggaatc gatgacgaca aagtaccgca acagtcatta tggaatcgga
     5221 tcggtgatgc catccgcgaa gttttggata caacaacaat caaagatttg gtggaatcgg
     5281 acgaatccac tgatctcgat ggatatatgt tctacatttg aagctataca ctaaaggagt
     5341 cttactatgc aaacgatata tttggatcat gccgccacta caccgatcca tccggaagtg
     5401 agcacggtat atgtgaaagc gatggatacg gtttttggca atccgtcaag catccatagc
     5461 tttggacggt cttcccgcaa attgctggac gatgcacgta aagcgatagc ggaggcaatc
     5521 catgccgagc cgaatgaaat catctttaca tccggcggca cggaagctga taatgtcgcc
     5581 atttttggta ccgccgctgc aatgaaggaa aagggaaatc atattattac gactgccatt
     5641 gagcatcatg ccgttctgaa ctcttgcaag gaattggaga agaacggctt tcacgtcacc
     5701 tatttgccgg tcgatgaaaa tggtagggtc agtgccgaac aggtagctga ggcgcttacc
     5761 gaccaaacgg tgctcgtcac ggtcatgtac ggcaataatg aagtcggtac tatacagccg
     5821 atcaaggaga ttggcgaaat cgtcaaggac catcaagcag ttttccatac ggatgcagtc
     5881 caagcattcg gcattatgga tctggatgtg aatgaactgc aagtcgatct gctgagcgct
     5941 tctgcccata aattgaatgg acccaaaggg gttggtttcc tgtaccaacg gaaagggctc
     6001 cagacaacac cacttcaatt tggaggggaa caggaacgga aaaggcgtgc aggaactgaa
     6061 aatgtccctg caattgcagg gtttgcagaa gctataaaga ttgccagaaa tgaaatggat
     6121 gaaaatcatc gaaaatatga tcggtataaa acgattctta ctgacgtttt tattcaagaa
     6181 ggaatcgtct ttgaagtgaa tgcagataaa gtggagcggc tgccgcatgt tgtgaacatc
     6241 agcttccccg gaaccgaaat tgaatccttg cttgtcaact tggacatggc gggtgtcgcg
     6301 gtatcgagcg gttcggcttg tacagcaggg tcactagagc catcacatgt attatcagcg
     6361 atgtggggaa gcgattccgc aaaattgcgc aattcggtgc gcttcagttt cggtttgggg
     6421 ctcgatgaag atgcggtccg tgaagccgca atgaaaacgg cgcaaattat taagcgtctt
     6481 gtgaaatgaa atgaaaaagg atgaatgata tgagaactgc aaataaacca atctccgaaa
     6541 ctcgggtagt cattggaatg tccggaggag tcgactcgtc tgtcgccgcc ctgttattaa
     6601 aggaacaagg ttatgacgtc atcggcatct tcatgaaaaa ctgggacgat actgacgagt
     6661 tcggtgtttg cacagcaacc gaagattacg aagacgtcat tagcgtgtgc aatgaaatcg
     6721 gaattcccta ttatgccgtt aacttcgaac agcaatattg ggataaagtc ttcacttatt
     6781 tcttagatga atataaagcc ggcagaacgc cgaatccgga tgtgatgtgt aacaaggaaa
     6841 taaaatttaa agctttcctt gaccatgcaa tgagccttgg ggcggatttc cttgcgacag
     6901 gtcactatgc ccaagtcgtc gaaacggatc aaggtgtggc gatgctccga gggaaagatg
     6961 agaacaagga tcagacgtac ttccttaacc ag
//
LOCUS       DASULI010000104         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_40412, whole
            genome shotgun sequence.
ACCESSION   DASULI010000104 DASULI010000000
VERSION     DASULI010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40412"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1839
                     /gene="fliF"
                     /locus_tag="VFX34_02285"
     CDS             265..1839
                     /gene="fliF"
                     /locus_tag="VFX34_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX5563763.1"
                     /translation="MKERLTKIGTDLKQFWSSRTKRQKTTYIGSAVAVIIFAALLTYF
                     LSRTEFVPLYSDVSRAEIGRIKEELDVQGVPSQIAPGGTSILVPKERVDDLLVTLAAE
                     GYPNTGTIDYSFFSQNAGFGTTDNEFNMIKLDTMQNELANLIKGIQGVKDANVMITLP
                     TPSVFLNETVQNASAAIMLKTEPGHQFTESQITALYNLVSKSVPNLSTDDIVIMNQYS
                     EYFDLKSSDQSIGMNVVNQMDLKKTIERDLQRKVQMMLGTMIGQDKVIVSVTTDIDFK
                     QENRQENLVDRVDEDLDSLAISVQRITESFSGDGAVAGGVPEAEDPTDNRTNFVEGNT
                     ANGDYERLEETINNEVNRIRKDIVESPYKIRDIGIQVMMEPSEGINVDQDIRNILGTI
                     IKTSIDKNYVPTDANEQYWQGKIAITTQVFNGKMEMVPQKGQSIIPWWVYAVGAALLV
                     VIAILIYAYFRKRRQEAELEEAMILEEQREALHVEDINMEHETEATLRKKQLEKMAKD
                     KPEEFAKLLRTWIAEE"
     gene            1854..2867
                     /gene="fliG"
                     /locus_tag="VFX34_02290"
     CDS             1854..2867
                     /gene="fliG"
                     /locus_tag="VFX34_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX5563764.1"
                     /translation="MVRKDKGMTGKQKAALLLISLGPEVSAAVYKHLTEEEIERLTLE
                     ISGVKKVDSSVKEEIIEEFHNIALAQDYISQGGIGYAKTVLEKALGKEHAQAIINRLT
                     SSLQVRPFDFARRAEPSQILNFIQNEHPQTIALILSYLEAEQAGMILSSLPQEMQADI
                     AKRIATMDSTSPEVISEIEAVLERKLSSTVTQDFTETGGVDAVVQVLNGVDRATEKTI
                     LDALEIQDPELAEEIRKRMFVFEDIVTLDNRSIQRIVRECENEDLILSMKVSSEEVRE
                     ILFKNMSQRMAESFQEEMEVMGPVRLRDVEEAQARIVSIIRRLEDSGEIIIARGGGDD
                     IIV"
     gene            2860..>3537
                     /locus_tag="VFX34_02295"
     CDS             2860..>3537
                     /locus_tag="VFX34_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HEX5563765.1"
                     /translation="MSNIFRSSRTISDEGSVKEITIRNLNMPTIDGKEEVISKEALFL
                     ERDRLLNEARRQIEIEKVELEQLRQMTQEDIASMQAAWEQEKAALQQQAYEEGFQVGY
                     EEGRNKSISDMSDSVKAANEVTAQSKANASKYLDEQERIILEIGMKTAERILGKTLEE
                     DEEAFLSIVKRGLKEAREMKEIKLYVPVSCYELVSSNRAELASIFPPDIPFLIFANED
                     FDRDDCII"
BASE COUNT         1109 a          716 c          890 g          822 t
ORIGIN      
        1 gtgctcgtaa cgcttcactt ttactcgcaa acgccgttct tcgtaacggc agagcaaaga
       61 tgtgctcgta acgcttcgct tttactcgca aacgccgttc ttcgtaacgg cagggcaaag
      121 atgtgctcgt aacgcttcac ttttactcgc aaacgccgtt cttcgtaacg gcagagcaaa
      181 gatgtgctcg taacgcttca cttttactcg caaacgccgt tcttcgtaac ggcgttgact
      241 atgtaagcag accgggggat cagaatgaaa gaaagattga cgaaaattgg gactgacttg
      301 aaacagttct ggtcgagtcg cacgaaacga caaaaaacaa catatatcgg atccgcggtt
      361 gctgtcatta ttttcgctgc acttttgaca tatttcctat cgaggacgga atttgtccct
      421 ctttatagtg acgtttcacg tgcggaaata ggccggataa aagaagagct ggatgtgcaa
      481 ggtgttccga gccaaattgc acccgggggt acatccatcc tcgttccaaa agaacgtgtg
      541 gacgaccttc ttgtgaccct ggccgcggaa ggatatccga atacgggaac tatcgattat
      601 tcgttcttct cccagaacgc cggttttgga acgacggata atgaattcaa tatgatcaaa
      661 ctcgatacaa tgcaaaatga gttggcgaat ttgatcaaag gaattcaagg cgtcaaagat
      721 gcgaatgtga tgatcacatt gccgacgcca agtgtctttt taaatgaaac tgtccaaaat
      781 gcaagcgctg caatcatgtt gaagacggaa cctggtcatc agtttacgga atcccaaatt
      841 actgccttat ataatttagt atcaaaaagt gtaccgaatt tatctactga cgatatcgtc
      901 attatgaacc agtattccga atacttcgat ttaaaatcat ctgaccaatc catcggtatg
      961 aatgttgtga accaaatgga tttgaaaaag acgattgaac gtgatcttca gcgaaaagtg
     1021 caaatgatgc taggtacgat gatcggtcaa gacaaagtaa tcgtctcggt tacgaccgat
     1081 atcgacttca agcaggaaaa caggcaggag aaccttgtcg accgtgtgga tgaggacttg
     1141 gacagtctgg caatcagcgt gcagcggata acggagtcat tctccggaga tggcgcagta
     1201 gcagggggcg taccggaagc ggaagacccg acggataacc gtacaaactt cgttgaaggc
     1261 aacacagcaa acggagatta tgaacgttta gaagagacca tcaataatga agtgaaccga
     1321 attcgaaaag atatcgttga gagtccttac aaaattcgtg atatcggcat acaagttatg
     1381 atggaaccgt ccgaagggat taacgtagac caagacatac gtaacattct agggacaata
     1441 attaagactt caattgataa aaactatgtg ccgacggacg caaatgaaca gtattggcaa
     1501 ggcaagattg caattaccac acaagttttc aatgggaaaa tggaaatggt tcctcaaaaa
     1561 ggccaaagca tcatcccatg gtgggtatac gcagtcggtg cagctttact ggttgtcata
     1621 gccatcctga tatatgctta cttccgcaaa cgcagacagg aagccgaatt ggaagaagcg
     1681 atgatacttg aagagcagcg tgaagcattg catgtggaag atatcaatat ggaacacgaa
     1741 acggaagcga cacttcgcaa gaagcaactt gaaaagatgg cgaaggataa gccggaagaa
     1801 tttgctaaat tattaagaac gtggattgcc gaggaatgac ggagggatga acagtggtaa
     1861 ggaaagataa agggatgacg ggaaagcaaa aagcggcact cctgctcata tcgctaggtc
     1921 cggaagtttc ggcagccgtc tataagcatt tgaccgaaga agagatcgaa cgtctgacgc
     1981 ttgaaatttc cggcgtcaag aaagtcgact cctccgtaaa agaagagatt atcgaggaat
     2041 tccataatat tgcacttgcc caagattata tctcccaagg cggaatcggc tatgcgaaga
     2101 ctgtgttgga aaaagcgtta gggaaagagc atgcccaagc aatcatcaat cggttgacat
     2161 cctctttaca agtaaggccg ttcgattttg caagaagggc ggaaccgagt caaatactca
     2221 atttcatcca gaatgagcat ccacagacaa tcgctctcat cctgtcatat ctggaagcgg
     2281 aacaggcggg gatgatcctg tcatctctcc cgcaggaaat gcaggcggat atcgcaaaaa
     2341 ggatagcgac catggattcc acttcccccg aggtcatcag cgaaatcgaa gcggttttgg
     2401 aaaggaagct atcttccaca gtcactcaag atttcacgga gacgggcggg gtcgatgccg
     2461 tggtacaagt attgaatgga gtcgacaggg ctaccgaaaa gacgattctg gatgcgctcg
     2521 aaatccagga tccggagctc gcggaagaaa tccggaaaag gatgttcgta ttcgaggata
     2581 tcgtcacatt ggacaatcgt tccattcagc gcattgtacg tgaatgtgaa aatgaagatc
     2641 ttattttatc aatgaaagtg tccagtgaag aagtaaggga gatcctcttt aaaaacatgt
     2701 cccaacggat ggcagaatca ttccaagaag aaatggaagt aatgggtcct gtccgtttgc
     2761 gtgatgtcga agaggcacag gcacgaatcg tatccatcat cagaagactg gaagattcag
     2821 gcgagatcat catagcccgc ggtggagggg atgacatcat tgtctaacat ttttcgttct
     2881 tcccgaacga tttccgatga aggcagtgtc aaggaaatca caatccggaa tttgaatatg
     2941 cctacaatcg atgggaaaga ggaagtgatt tcaaaggaag cactttttct ggagcgcgat
     3001 cggttgttga atgaagccag aaggcagatt gagattgaaa aggttgaatt ggaacaactg
     3061 aggcagatga cacaggaaga tatcgcctcc atgcaagctg cctgggaaca ggagaaggcg
     3121 gcgcttcagc aacaggctta tgaagaggga ttccaagtag gatatgaaga aggaaggaat
     3181 aagtcgatat ccgatatgag cgattctgtg aaagctgcaa atgaagtgac tgcccaatcg
     3241 aaggcaaatg cctcgaaata cttagatgag caggaaagaa tcatattgga aatcggaatg
     3301 aagaccgcgg aacggatttt agggaaaaca cttgaagaag atgaagaagc tttcctgtca
     3361 attgtcaaaa gagggttgaa ggaagcaagg gaaatgaaag aaattaaact atatgtccct
     3421 gtttcctgtt atgaactcgt ctcttcgaat cgtgctgaac ttgcatccat tttcccgccg
     3481 gatatccctt tccttatctt cgcgaatgag gatttcgaca gggacgattg cattatt
//
LOCUS       DASULI010000105         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_53923, whole
            genome shotgun sequence.
ACCESSION   DASULI010000105 DASULI010000000
VERSION     DASULI010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53923"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFX34_02300"
     CDS             complement(<1..609)
                     /locus_tag="VFX34_02300"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5563766.1"
                     /translation="MANVKREDQAVYDAIMAEKKRQQANIELIASENFVSQAVMEAQG
                     SVLTNKYAEGYPGKRYYGGCEHVDVVENIARDRLKEIFGAEYANVQPHSGAQANMAVY
                     FTILEPGDTVLGMNLSHGGHLTHGSPVNFSGIQYNFVEYGVSKDDEKIDYEDVRQKAL
                     EHKPKLIVAGASAYPREIDFAKFREIADEVGAYFMVDMAHIAG"
     gene            complement(620..1180)
                     /locus_tag="VFX34_02305"
     CDS             complement(620..1180)
                     /locus_tag="VFX34_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEX5563767.1"
                     /translation="MDALQLWKLQLEQLLVEIEEQIELEPDSIFVVGCSTSEVAGKRI
                     GTAGALEIAEVFYKPLQLFAERNQLHLAFQGCEHINRALTVERKTARTFHLEPVTVIP
                     DVSAGGSMSAYAYRQMKQPVVVEEIRANAGIDIGQTLIGMHLKRVAVPVRTSISKIGD
                     AIVTAATTRPKLIGGERAIYKLEEGE"
     gene            complement(1201..1638)
                     /gene="rpiB"
                     /locus_tag="VFX34_02310"
     CDS             complement(1201..1638)
                     /gene="rpiB"
                     /locus_tag="VFX34_02310"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX5563768.1"
                     /translation="MKIAISSDHGGNNLRREIISLLTELGIEYEDFGPGSDESVDYPD
                     YAKPVADGVAKGNFDRGILICGTGIGMSIAANKVKGIRCALVHDVFSAKATRGHNDSN
                     VLAMGERVIGPGLAREIVETWLKAPFEGGRHERRIGKLMDMED"
     gene            complement(1811..>2116)
                     /locus_tag="VFX34_02315"
     CDS             complement(1811..>2116)
                     /locus_tag="VFX34_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5563769.1"
                     /translation="DRANETSGNMESMADTVNVMADSVVEITKFIENQLKNIETTATQ
                     SQDVAAIAEETSAAALEVGSATGEQAKSIEQVDEMAVELKKQSEQLYSVISRFDLTK"
BASE COUNT          506 a          580 c          415 g          615 t
ORIGIN      
        1 acccgcgatg tgcgccatat cgaccataaa gtacgcgccg acttcatccg cgatttcacg
       61 gaatttcgca aagtcgattt cacgtgggta cgcacttgca cccgcaacga tcaatttcgg
      121 cttatgttca agtgcttttt gacggacatc ttcgtaatca atcttttcgt cgtctttgct
      181 aacgccgtat tcaacgaaat tgtattgaat tccagagaag ttaaccgggc taccgtgtgt
      241 caagtgaccg ccgtgggaca ggttcatccc aagcaccgtg tcgcccggct caagaattgt
      301 gaaataaacc gccatgttgg cttgtgcacc agagtgtggt tgaacgtttg cgtattccgc
      361 accgaagatt tctttcagac ggtcgcgtgc aatattttca acgacatcta cgtgttcaca
      421 gccaccatag taacgctttc ctggatagcc ttccgcatat ttattcgtca gtacagagcc
      481 ttgtgcttcc atgactgcct gggaaacaaa gttctctgac gcgattaatt cgatgtttgc
      541 ttgctggcgc tttttctccg ccatgatagc atcatataca gcttgatcct cacgttttac
      601 gtttgccaat tccatgatct cattcccctt cttctaattt gtatattgca cgctctccgc
      661 cgattaattt aggtctcgtc gtagcagcag taacaatcgc atcaccaatt ttgctgattg
      721 aggtccgaac cgggaccgcc actctcttca aatgcatccc aattaatgtc tgtccgatgt
      781 cgattcccgc attcgcgcga atttcttcca caacgaccgg ttgcttcatc tgacgatatg
      841 catatgcact cattgatcca ccggcagaca catcgggaat gactgtaacc ggctccaagt
      901 ggaatgtacg agccgttttc cgttcaaccg taagtgcacg attaatatgt tcacagcctt
      961 ggaacgcgag atgaagctga ttacgttcag cgaataattg gagcggcttg taaaacacct
     1021 cggcaatttc aagggcaccc gctgttccaa ttcgctttcc tgcaacttca gaggtagaac
     1081 agcccacaac aaatatgctg tccggctcca actcaatctg ctcttcgatt tccaccagaa
     1141 gctgctccag ttgaagcttc cacaactgca aagcatccat tctgtcgccc ccctgtttgc
     1201 ttagtcttcc atgtccatca acttgccgat tcgacgctca tggcgcccgc cttcaaatgg
     1261 cgcttttagc catgtttcta caatttctct tgcaagaccg ggaccgatga cacgctcccc
     1321 cattgcgagt acgttagagt cattatgccc ccgtgtcgct tttgcgctaa atacgtcatg
     1381 cacaagtgca caacgaatgc ccttcacttt gttcgcagcg atcgacatgc cgattccggt
     1441 tccgcaaatg aggattcctc tatcgaaatt cccttttgca accccgtccg ctaccggttt
     1501 tgcgtagtct ggatagtcaa cagactcatc tgagcccgga ccaaaatctt catactcaat
     1561 tccaagttca gttagcaatg aaatgatttc acggcgaaga ttgtttccgc catgatcgga
     1621 agaaatcgcg attttcatcg tgtccctctt ttcctaattt acttagtatt cattctacca
     1681 tttttcatcg acaaaaaata cagccctgca tttgcagggc tgaaaaaatt atagtatgaa
     1741 taatgttttt tcatcaaatt cattgaacca ttgaccttat tcgcgaataa agtcaataga
     1801 ataaaaccaa ttatttcgtt aaatcaaacc ggctaatgac gctgtatagc tgctcggact
     1861 gcttcttcaa ttcaaccgcc atttcgtcga cctgctcgat ggatttcgct tgttcaccag
     1921 ttgcagagcc gacctcaagt gcagccgccg acgtttcctc tgcgattgcc gctacatcct
     1981 gcgattgcgt tgccgtcgtt tcaatattct ttaattgatt ctcaataaac ttcgtaattt
     2041 caacaacaga gtccgccatg acattcactg tatctgccat cgactccata ttgccgctcg
     2101 tctcgttcgc acggtc
//
LOCUS       DASULI010000106         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_646198, whole
            genome shotgun sequence.
ACCESSION   DASULI010000106 DASULI010000000
VERSION     DASULI010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646198"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFX34_02320"
     CDS             <1..449
                     /locus_tag="VFX34_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEX5563770.1"
                     /translation="KEIVIRSATLFDVDIDANAAVEIARRSRGTPRIANRLLRRVRDY
                     AQVRGDGNITMAIAKEALEMLQVDSHGLDHIDHKLLASMIERFRGGPVGIDTIAASIG
                     EESITIEDVYEPYLLQIGFIQRTPRGRIATRLAYDHFGYEPPEALS"
     gene            494..1534
                     /gene="queA"
                     /locus_tag="VFX34_02325"
     CDS             494..1534
                     /gene="queA"
                     /locus_tag="VFX34_02325"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010303554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX5563771.1"
                     /translation="MDVNDFDFELPESLIAQTPLKDRTASRLMVLDKVTGEVKHRQFR
                     QIVDELQEGDVIVLNDTRVLPARLIGTKEDTGASIEVLLLKETGSDEWETLVKPAKRV
                     KVGTVVTFGDGRLEAECTEILEQGGRIFKFRYEGIFYEILDSLGQMPLPPYITETLDD
                     QSRYQTVFAKERGSAAAPTAGLHFTEEILQEIKEKGVGVEFITLHVGLGTFRPVSVDS
                     IEDHTMHSEYYHVTEKAADAINEAKAKGGNVIAIGTTSARTLETIASQNDGKIVPTSG
                     WTSIFIYPGYQFSILDGMLTNFHLPKSTLIMLISALASREIVLAAYKEAVKEKYRFFS
                     FGDAMFIKPHKK"
     gene            1573..2712
                     /gene="tgt"
                     /locus_tag="VFX34_02330"
     CDS             1573..2712
                     /gene="tgt"
                     /locus_tag="VFX34_02330"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016201679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5563772.1"
                     /translation="MAAITYEHIKTCKQTGARLGIVHTPHGSFETPAFMPVGTQATVK
                     TMSPEELKEMGAGIILGNTYHLWLRPGNDIIKEAGGLHKFMNWDRPILTDSGGFQVFS
                     LSEFRKIEEEGVHFRHHLNGSKLFLSPEKAMEIQNDLGSDIMMAFDECPPYPATYEYM
                     KASVERTSRWAERCLKAHARPEDQGLFGIVQGGEFEELRKQSAKDLISLDFPGYAIGG
                     LSVGEPKDVMNRALEFTTPLLPANKPRYLMGVGSPDSLIDGAIRGVDMFDCVLPTRIA
                     RNGTLMTSEGRLVVKNAKYERDFGPLDPNCDCYTCKNYSRAYIRHLIRANETFGIRLT
                     SYHNLYFLLRLMEQIREAIRNDRLGDFREEFFEQYGFNKPNAKNF"
     gene            2749..>2942
                     /gene="yajC"
                     /locus_tag="VFX34_02335"
     CDS             2749..>2942
                     /gene="yajC"
                     /locus_tag="VFX34_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5563773.1"
                     /translation="MNGDMLGTLLPIIAMFAIMWFLLIRPAQKRQKQTKTMQEGLKRG
                     DKVITIGGLHGVIDAVDDTSV"
BASE COUNT          841 a          668 c          756 g          677 t
ORIGIN      
        1 tgaaggaaat cgtcatcaga agcgcaaccc tgttcgatgt cgatatcgat gccaatgcgg
       61 cggttgaaat cgcaaggcga tcgaggggta cgccccgtat tgccaaccgg ctcctgcgaa
      121 gagtgcggga ttacgcccaa gtgcgtggtg acggaaacat tacaatggct atcgcgaagg
      181 aagcgcttga aatgctgcaa gtcgattcac acggtctcga tcatatcgac cataagttgc
      241 tcgccagcat gatcgaacga ttccgcggag gacctgtcgg aattgatacg attgccgcaa
      301 gcatcggtga agagtccatc acaatcgagg acgtctatga gccctacttg ctgcaaatcg
      361 gtttcatcca aagaacccct cgcggcagga ttgcaaccag gctcgcatac gatcacttcg
      421 gctacgaacc gcctgaagca cttagctgat gttagatgtt caatatttga tatagaagga
      481 gttcacccgc attatggatg taaatgattt tgattttgaa ttgccggaaa gtctcatcgc
      541 ccaaacgccg cttaaagacc gaactgcgag caggctaatg gttctggata aagtgacagg
      601 cgaagtcaaa catcgccagt tccgccaaat tgtcgacgag ctgcaagaag gagacgtcat
      661 cgtactgaat gatacacggg tcctgcctgc ccgtttgatt ggcacaaaag aagacactgg
      721 cgcatcaatc gaagtgcttc ttttgaaaga gaccggctca gacgaatggg agacactcgt
      781 aaagcccgca aaacgcgtga aagtcggaac ggtcgtaacg ttcggggacg gcagattgga
      841 agccgaatgc accgaaatcc tcgagcaagg cggaaggata ttcaaattcc gctatgaagg
      901 cattttctat gaaatactcg attccctagg acagatgccc cttcctcctt atattacgga
      961 gacccttgat gatcaatcgc gctaccagac cgtctttgcg aaagaaaggg ggtcggcggc
     1021 ggctcctact gccggccttc atttcaccga agaaatcctg caggaaatca aggaaaaagg
     1081 tgtcggtgtc gagttcatca cgttgcacgt cggtttggga acattccgac ctgtgagtgt
     1141 cgattcgatc gaggatcata caatgcattc agagtattat cacgtgaccg agaaggcggc
     1201 agacgcaatt aacgaagcca aagcaaaagg cggaaacgtc atcgcaatcg gcactacttc
     1261 agcgaggacg ctggaaacga tcgcttcgca aaacgacgga aaaatcgtcc ctactagcgg
     1321 gtggacttcg attttcatct acccagggta ccagttcagc attcttgacg gtatgctgac
     1381 gaatttccat ttgccgaaat cgacgttgat catgctcatt tccgcgcttg cctcacgaga
     1441 aattgtgcta gcagcctata aagaggcagt gaaggaaaaa taccgcttct tcagtttcgg
     1501 ggatgcgatg ttcatcaagc cccacaaaaa gtaatgaggt actaatcctc aaaatagaaa
     1561 ggatcacacg aaatggcagc aattacttac gaacatataa aaacgtgcaa acagacaggg
     1621 gccaggctcg gcatcgtcca tacgccccac ggctcttttg agacgccggc attcatgcct
     1681 gtcgggacgc aagcaacggt aaagacgatg tcaccggagg aactgaagga gatgggagcc
     1741 ggcataatcc tcggcaatac ctatcatctt tggctgcgac cgggaaatga tattattaaa
     1801 gaggcgggcg gcctccataa attcatgaat tgggatcgtc cgattctgac ggattcaggc
     1861 ggcttccaag tgttttcatt gagtgaattc cggaaaattg aagaggaagg tgtccatttc
     1921 cgtcatcatt tgaatggcag caagcttttc ctaagtcctg aaaaagctat ggaaatccag
     1981 aatgatcttg gttcagatat tatgatggca ttcgatgaat gtccgccata tcccgcgaca
     2041 tacgaatata tgaaggcaag tgtggaaaga acttcaagat gggcggaacg ttgcctaaaa
     2101 gcgcatgcac gtccggagga tcaaggacta ttcggaattg tccaaggggg cgaattcgag
     2161 gagcttcgaa aacaaagcgc gaaagatctc atctcacttg actttcctgg ctatgcaatc
     2221 ggcggattat ccgtcggtga gccgaaagat gtcatgaacc gagctcttga atttacgact
     2281 ccgctgttgc cggcaaacaa accgcgttat ttgatgggcg tcggatcgcc ggattccttg
     2341 attgacgggg caatccgagg tgtggacatg ttcgactgcg tattgccgac acgtatcgcc
     2401 aggaacggga cattgatgac aagcgaaggg cgtctcgtcg tgaaaaatgc aaaatacgaa
     2461 cgtgatttcg gtccattgga tccgaattgc gattgctata catgcaaaaa ctatagcagg
     2521 gcatatatcc gccacttgat ccgtgcaaat gaaacttttg gaatcaggtt gacgtcctat
     2581 cataatctgt acttcttatt gcgattgatg gaacagatca gggaagcgat ccgcaatgac
     2641 cggctcggtg atttccggga agagtttttc gaacaatatg gatttaataa accaaatgca
     2701 aaaaactttt gaatcttgta taataaacca agaagagggg ggaataaaat gaatggtgat
     2761 atgttaggta cgttgcttcc aattattgca atgttcgcaa tcatgtggtt cttattgatt
     2821 cgacctgcac aaaagagaca aaagcagaca aagacgatgc aagaaggttt gaagcgtggc
     2881 gataaagtca ttacaattgg cggtcttcac ggcgttatcg atgcagtcga cgatacatcc
     2941 gt
//
LOCUS       DASULI010000107         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_646137, whole
            genome shotgun sequence.
ACCESSION   DASULI010000107 DASULI010000000
VERSION     DASULI010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646137"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2031)
                     /gene="fdhF"
                     /locus_tag="VFX34_02340"
     CDS             complement(<1..>2031)
                     /gene="fdhF"
                     /locus_tag="VFX34_02340"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX5563774.1"
                     /translation="IDLVKDVEPGYSGIMAISDAEAAMRETRTKKTKTVCTFCGVGCS
                     FEVWTKDRKILKIQPVSEAPVNAISTCVKGKFGWDFINSEERLTTPLIRKGDSFVEAT
                     WNEALDLVAERLGAIHKEYGEDSVGVISSSKITNEENYLIQKLARQVFKTNNVDNCSR
                     YCQSPATDGLFRTVGMGGDAGTIKDISEAGLVIIIGANPAEGHPVLATRVKRAHKLHG
                     QKLIVADLRKHEMAERSDIFISPKQGTDQVWLMAVTKYMIDKGWHDSAFIQENVHHFE
                     EFKDVLKTYTLDFAEHITGVPKDMLIRTAEMIRDADGTCILWGMGVTQNTGGSDTSAA
                     ISNLLLATGNYRRPGAGAYPLRGHNNVQGACDMGTLPGWLPGYQHVTDNAAREKFERA
                     YGVEIGNKPGLDNIQMLHAVDEGIMKGMYIVGEDMALVDSNANHVHDVLSKLDFLVVQ
                     DIFFSRTARYADVILPAVPSLEKDGTFTNTERRVQRLYKALPELGDAKADWWIVQEIA
                     TRLGADWQFTHPSEIFAEMASLSPLFSKADYSNMENWDSFLWGSLNGDSTPLLYVDGF
                     NFPDGKARFALSDWVEPAHYDDEFDLHINNGRILEHFHEGNLTNKSGGIQSKVPEVFV
                     EVSPELAKERGVLSGSLVRLVSPHGALRLPALVTDRVKGNELFLPMNSVEKES"
BASE COUNT          511 a          552 c          415 g          553 t
ORIGIN      
        1 gattcttttt cgactgagtt catcggaagg aataattcat tccctttcac cctgtcagtc
       61 actagagcgg gcagccggag ggctccgtgc ggtgacacaa gccgtactag cgaaccgctg
      121 agcacgcctc tttccttcgc aagttccgga gaaacttcaa cgaaaacttc aggtacctta
      181 gactggatgc ctcccgattt gtttgtcaga ttcccttcat ggaaatgctc aagtatccgg
      241 ccattattga tgtgcaaatc aaattcatcg tcataatgtg caggctccac ccaatcggat
      301 aaggcaaatc tcgctttccc atccgggaaa ttgaatccgt cgacatagag caatggcgtg
      361 ctgtctccat tcaagcttcc ccacaaaaag ctatcccagt tttccatatt gctgtaatcc
      421 gccttgctga ataatggcga aagactggcc atttcagcaa agatttcact tggatgtgta
      481 aactgccaat cggccccgag tcttgtagcg atttcctgga caatccacca atccgccttc
      541 gcatccccta attccggcaa tgctttatac aatcgctgca ctcttcgttc cgtattcgta
      601 aatgtcccat ccttctccaa agaaggaact gccggaagga tgacatctgc atatctagct
      661 gtcctcgaga agaagatatc ctggacgact aggaagtcga gtttcgaaag aacatcatga
      721 acatgattcg cgttggaatc gacgagagcc atgtcttccc cgacgatata catccctttc
      781 ataatgcctt catcgaccgc atggagcatc tggatattat ccagtcccgg tttattgcca
      841 atttcaaccc cgtatgcgcg ctcgaatttt tcacgtgcag cattatccgt cacgtgctga
      901 taacccggca gccaacctgg gagagtcccc atatcacaag caccttggac attgttatgg
      961 ccacgaagcg ggtaagcacc tgcgcctggc ctgcgataat taccagtagc aagaagtaaa
     1021 ttggatatcg cagcggaagt atccgatcca ccggtatttt gagtgactcc cattccccac
     1081 aaaatacatg ttccatctgc atcgcggatc atttcagccg tgcggatcaa catatctttc
     1141 ggtacacctg taatatgctc agcaaaatca agcgtatatg ttttcagtac atctttgaat
     1201 tcctcaaaat gatggacatt ttcttggatg aatgccgaat catgccaacc tttatcaatc
     1261 atatattttg taacggccat cagccaaact tggtcagttc cctgcttagg gctaatgaag
     1321 atatcagatc gctccgccat ttcatgtttg cgcaaatcag cgacgatcaa tttctgtcca
     1381 tgcagtttat gggcccgttt tacacgcgtg gcgaggacgg gatgcccttc agccggattc
     1441 gctccaatga tgatgacaag gcccgcctct gaaatgtctt taatcgtgcc tgcgtcaccg
     1501 cccattccaa ccgtccggaa taacccatcg gtcgccggtg attgacagta gcgcgaacag
     1561 ttgtcgacat tattcgtctt aaacacttgc cgagccagct tttgaatcaa atagttttcc
     1621 tcattggtga ttttagaaga tgaaatgaca cccacactat cttccccata ttccttatgg
     1681 atggccccta aacgctctgc aaccaaatcc aatgcttcat tccaagtggc ttcaacgaaa
     1741 gaatcccctt tccggatgag cggagtcgtc aatcgttctt cgctgttaat gaaatcccag
     1801 ccgaattttc cttttacgca agtcgagatt gcattgacag gcgcttcgga aactggttgg
     1861 atcttaagga ttttcctgtc ctttgtccat acttcaaatg aacacccgac cccacagaat
     1921 gtacataccg tcttcgtttt cttcgtcctt gtttcccgca tcgcagcttc tgcatctgaa
     1981 atggccatga tgccgctata gccaggctcg acatccttaa caaggtcaat c
//
LOCUS       DASULI010000108         6264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_4806, whole
            genome shotgun sequence.
ACCESSION   DASULI010000108 DASULI010000000
VERSION     DASULI010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4806"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFX34_02345"
     CDS             complement(<1..554)
                     /locus_tag="VFX34_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5563775.1"
                     /translation="MGTGKRLVHYALAYKKLLITGIILLGFAVAADLMGPMIAKKIID
                     DHIATSVDQTINFTPIAKLLAVFFSLAVVTAILRYFQYLLLQNAANRIIQKMRNELYG
                     HIQTLPIRYFDNLPAGKVVARITNDTEAIRNLYVTVVSQFAISGMYMLGIFIALFYLD
                     PKMGAITLFVLPVLYIWMKLYRRFA"
     gene            complement(556..2295)
                     /locus_tag="VFX34_02350"
     CDS             complement(556..2295)
                     /locus_tag="VFX34_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563776.1"
                     /translation="MFSVLFKLKWFFKENRKRYTVALILLMMTNVLVILPPWLIGKSI
                     DTIYTNTMTTHLLALFIGAMFLIMLCSYIGNFVWQYQLFGGAYVIERQLRSRLMRHFL
                     KMTPTFYEKNKTGDLMARSTNDLRAISETAGFGIMTLVDSTLYLGTLIITMGFVVSWK
                     LTLAAILPLPILAFILQVLGKKIHERYMTAQEAFGDLNDNVLEAVAGVRVVRAYVQER
                     ASEQRFADMTEDVYKKNMHVEKIDALFMPFSKVLTALTYMIGLGYGAYLVSAGEMTLG
                     NLVTFNVYLGMIVWPMFAIGELINVLQRGNASLDRVMETLNYEEDVKDPDEPIDIDQP
                     ESVGFNDVSFTYPMSHAVNLDHIELQLDRGQTLGIVGKTGSGKTTFVKQLLREYPAGD
                     GEIVFSDVALQSLKKDQVRDWIGYVPQDHVLFSRSVRDNILFGRPEATEEDIAESIRL
                     SYFEKDLQMLPAGLDTLVGEKGVALSGGQKQRISIARALVKNPEILILDDSLSAVDAK
                     TESKIIENIQSERSGKTTIITTHRLSAIQHADWIIVLDDGRVIEEGRHEDLLAKNGWY
                     KEQFDRQQIGEVE"
     gene            complement(2777..3958)
                     /gene="chrA"
                     /locus_tag="VFX34_02355"
     CDS             complement(2777..3958)
                     /gene="chrA"
                     /locus_tag="VFX34_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX5563777.1"
                     /translation="MKKQKNASEILRTSLKLGLTSFGGPAAHIGYFRDEYVKKKKWLD
                     DKMYADIVALCQFLPGPASSQVGIAIGLMRGGLLGGILSWIGFTLPSVLLLMAFAWLI
                     SSTATFDIGWIKGLKIVAVAVVAHALVGMSKSLTPDRARITIALIAAVLTLVIPTAVG
                     QIAIIVGAGLVGYALYRKEKAPKPESIDLSFGKRTGAAAWTIFFLLLIAVPAIRSFVE
                     NTFLSIFDIFYRVGSIVFGGGHVVLPMLEREVVPAGWMDAETFIAGYGAAQAVPGPLF
                     TLSGYLGQLMNGPSGAAIAVIAMFLPSFLLVIGTLPFWSAIRSKPGIQAALKGVNAAV
                     VGILLAALYDPVFTSSIGHPIDFGIAIIAFGLLMYYRMAPWLVVFITAALGALSHLLL
                     G"
     gene            4124..5458
                     /locus_tag="VFX34_02360"
     CDS             4124..5458
                     /locus_tag="VFX34_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5563778.1"
                     /translation="MEIAIRLAAFAALIALTAFFVASEFAIVKVRTTRINQLVAEGNR
                     RAITAKKVISNLDEYLSACQLGITITALGLGMLGEPTVKLLMKPVFSHFEIAPHAATI
                     LSFIIAFTVVTFLHVVVGELAPKTIAIQRAEAITLASAKPLILFYFIMFPVIKGMNGA
                     ARSIIRLFGFKTASESEMVHSEDELRLILTDSLKGGEINQSEYKFVNKIFEFDDRVAK
                     EIMVPRTEMMTIEKDVTLREMFEMMGVEQFTRYPVTDGDKDHVIGLVNMKNLLTAFIK
                     DPSTGDQPVSEYMQPIIRVIETMAIGDLLLKIQRERIHMAILMDEYGGTSGLVTIEDI
                     LEEIVGEIQDEFDMDEVPEVQMINENHYILDAKMLIENVNDVLDIDIDEEDIDTIGGW
                     FMSERFEAVPGEKIIEQGYEFTVKDVEGHHILYLEAMKNEKTNGTNDLQVES"
     gene            complement(5643..>6264)
                     /locus_tag="VFX34_02365"
     CDS             complement(5643..>6264)
                     /locus_tag="VFX34_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5563779.1"
                     /translation="NVNIIGGSHFVEEEDEEIYNIAYLFRRDGSIEKQYKIHITPNER
                     KWWGISQGDEIKVFDTDCGKIAIQVCYDIEFPELARIATDKGANIIFTPFCTEDRQGY
                     LRVRYCAQARAIENQIYTVISGTVGNLPQTENMDIQYAQSAIFSPSDFEFARDGIVGE
                     TNANLEMVLIGDVDLEILRRQRQDGTVKQLKDRRHDVYRVVYKKEQ"
BASE COUNT         1674 a         1596 c         1375 g         1619 t
ORIGIN      
        1 gcgaatcttc tatataactt catccaaata tagaggactg gcaagacgaa cagcgtaatg
       61 gcccccattt tcggatccaa atagaatagg gcgatgaaaa tcccgagcat atacatgccg
      121 ctgatcgcaa actgtgaaac gaccgtcaca tacaaattcc ggatcgcttc cgtgtcattc
      181 gtaatccgcg cgacgacctt ccctgccggc aaattatcga aataacggat cggcaacgtc
      241 tggatatgcc catacagttc gttccgcatc ttctggataa tccggtttgc tgcattttgc
      301 agcaataaat attggaagta tcgcagaatt gccgttacga ctgccagact gaaaaacaca
      361 gccagaagtt tggcgatcgg tgtgaaattg atcgtctgat caaccgaagt cgcaatatga
      421 tcgtcaatga tcttcttcgc aatcatcggc cccatcaagt ccgccgccac agcgaagcca
      481 agaaggatga ttcctgtaat taaaagcttc ttataagcaa gtgcataatg cactaaacgt
      541 tttccagtac ccatcttatt caacctcccc gatctgctgg cggtcgaatt gctctttata
      601 ccatccgttc ttcgcaagca agtcttcatg tcttccttct tcaatgacgc gcccatcatc
      661 gagcacgatg atccagtccg cgtgttggat tgcagataaa cggtgcgttg tgatgatcgt
      721 cgttttccct gaacgttccg attggatgtt ctcgatgatc ttcgattccg tcttcgcatc
      781 gactgccgaa agcgagtcat ccaggatgag gatttcagga tttttcacaa gcgcacgggc
      841 gattgaaatc cgttgctttt ggccgccgga taacgcaaca cccttttcgc cgaccaatgt
      901 atcgagtcct gctggcagca tctgcaaatc cttttcaaaa taggataagc ggatcgattc
      961 cgcaatatct tcttcggtag cttccggacg tccgaacagg atgttatccc ggacggaacg
     1021 cgagaatagc acatgatcct gtggcacata gccgatccaa tcacggactt gatccttctt
     1081 caatgactga agcgcaacat ccgagaagac gatttccccg tcacccgccg gatactcacg
     1141 caacagctgc ttgacgaacg tcgtcttccc gcttccggtc ttcccgacga tgccgagcgt
     1201 ctgcccccgg tccaattgga gttcgatatg atccaaattg accgcatgcg acatcgggta
     1261 cgtgaaactg acatcattga acccgacgct ttcaggctga tcgatatcga tcggttcgtc
     1321 aggatctttt acatcctcct cataattgag cgtctccatg acgcggtcca aggaagcatt
     1381 cccacgctgc aacacattga tcaattcgcc gattgcaaac atcggccaaa cgatcatgcc
     1441 caagtaaacg ttgaacgtaa caagattccc cagcgtcatt tcgcccgccg acactaaata
     1501 ggccccgtat ccaagcccga tcatatacgt caatgccgtc aacactttcg aaaacggcat
     1561 gaacagtgca tcgatctttt caacatgcat attcttcttg tatacatctt ccgtcatatc
     1621 cgcaaaccgc tgctccgaag cacgttcttg cacataagca cggacgaccc gtacacccgc
     1681 cacggcctcg agaacattat cattcaaatc cccgaatgcc tcttgagccg tcatatagcg
     1741 ttcgtggatc ttcttcccga gcacttgcaa aatgaaggcc aaaatcggca gtggcagaat
     1801 cgctgcaagt gtcagcttcc acgagacgac aaatcccatc gtgatgatca gcgtacctaa
     1861 atacaatgtc gagtcgacga gcgtcatgat tccgaagcct gccgtctctg aaattgcacg
     1921 tagatcattc gtcgaacgcg ccatcaggtc gcctgttttg tttttctcgt aaaatgtcgg
     1981 cgtcatcttg aggaaatggc gcatcagcct actcctcagc tgcctttcaa taacgtaggc
     2041 cccaccgaat agctgatatt gccagacgaa gtttccgata tacgaacaaa gcatgatgag
     2101 gaacattgca cctatgaata gcgcaagcag atgtgttgtc atcgtattcg tataaatcgt
     2161 gtctatcgac ttaccgatca gccacggagg caaaatgact agcacgtttg tcatcattaa
     2221 gaggatgagt gctaccgtgt atcttttgcg gttttctttg aaaaaccact ttaatttgaa
     2281 taaaaccgaa aacatgtctt caaccttctc tcttcaattc tataaactat ccactttcta
     2341 gagattccct cttcaatagt ttcctttgct gatttgcaat tttcatgatc agcgctcctt
     2401 tccaacattt ttatatctca cacgttaaac gtggaaacac ctgaagttta ttccaaagac
     2461 aaaaaaggca cacgcctcct ataagcgtgt gctcagaaaa agggacgact aagtcatgcc
     2521 gccctgttga atttcataga ctagagaaag tgcaggtcag cacggaattg atatttgatt
     2581 gttgtttgat aaaaaggact tttttcattt tccgcacctt cctcactatt tcgaatttcg
     2641 aataattcca cagtaccact gataatttcc atttgtcaat acatttctgt atatttacat
     2701 ggattgtttc aaaacgggca tgccaggaga agtcctcctg gcatttttca ttgtatgcac
     2761 gtaatgctct ccttattcac cctaacaata gatgagagag tgctcccaaa gccgccgtga
     2821 tgaacacaac aagccaaggt gccattctgt aatacattaa tagtccgaat gcgatgatgg
     2881 cgattccgaa atcgattgga tggccgatcg aacttgtgaa taccgggtca tacaatgcag
     2941 caagcaaaat cccgacgacg gctgcattca cacctttcag cgcagcctga atgccaggct
     3001 tcgaacggat ggcggaccaa aatggcaagg tgccgatcac taacaggaat gatggcagga
     3061 acatggcaat caccgctatc gctgctcccg atggtccatt catcagctgt cccaaatagc
     3121 cggacagcgt gaagagcggt ccaggcacgg cttgtgccgc cccataccca gcgatgaacg
     3181 tctctgcgtc catccagccc gccggcacga cttcccgttc aagcatcggc aatacgacgt
     3241 gacctccgcc aaaaacaatc gaaccgacac gatagaaaat atcaaaaatc gataggaatg
     3301 tattttcaac gaaagacctg attgccggga cggctataag caacaggaaa aatatcgtcc
     3361 atgccgccgc tcccgtcctc tttccgaatg acagatcgat cgattcgggc ttcggtgcct
     3421 tttccttccg gtacagtgcg tacccgacga gccccgcgcc tacgatgatg gcgatttgtc
     3481 cgactgcggt cggtatcact aatgtcagga cagccgcaat gagtgcgatt gttattcttg
     3541 cccggtcagg cgtcaatgat ttgctcatcc cgaccaatgc atgcgcaact acagcaactg
     3601 cgacaatctt caatcctttg atccacccga tgtcgaatgt tgccgttgag gatataagcc
     3661 aggcaaatgc cattaacagc agcaccgacg gcagcgtgaa accgatccag gaaaggattc
     3721 ctccgagtaa acctcctcgc atcaacccga ttgcaatccc gacttgcgag cttgcggggc
     3781 cgggcaggaa ctggcacaac gccacgatgt ccgcatacat tttatcatcg agccatttct
     3841 ttttctttac atattcatcc cggaaataac cgatatgggc tgcagggccg ccgaaagacg
     3901 tcagcccaag cttcagggat gtgcgtaaaa tctctgatgc atttttctgc ttcttcacaa
     3961 ctacacctcc atatgtactt tgtccattat acaagactta ggcaatacca tttgtttttt
     4021 tcttgtaaat aatttattat tatcttaact aaccatttta accttacagg aggtgtgcac
     4081 tttgttctac ccctcattcg ggcgcggggc aaagtaaact tatttggaaa ttgccatacg
     4141 attggcagct tttgctgcct tgatcgcact caccgcattt tttgttgcga gtgaatttgc
     4201 aatagtgaaa gtacgaacaa cgaggatcaa ccagcttgtt gcggaaggga accgccgggc
     4261 gatcacagca aagaaagtca tcagcaatct cgatgagtat ttatcagcct gtcaattggg
     4321 cattacaata acggctttag gacttggtat gcttggtgaa ccaaccgtga aactgcttat
     4381 gaagccggtt ttctcccatt ttgaaattgc cccgcatgct gcgacgattc tatctttcat
     4441 catcgcattt actgtcgtaa cctttttaca tgtggtagtt ggtgaattgg ctcctaaaac
     4501 gatcgcaatt caacgggcgg aagccattac cctggcgtca gcgaaaccgc tcattttatt
     4561 ttatttcatc atgttccctg tcattaaagg gatgaatgga gcagcacgtt ccatcatccg
     4621 cctattcggc ttcaaaacgg cttccgaatc ggaaatggtc cactccgagg atgaattgag
     4681 actgattctg accgatagcc tgaagggcgg ggaaatcaac cagtcggaat ataaattcgt
     4741 caataagata ttcgagttcg acgacagggt cgcgaaagag atcatggtgc cgcgtaccga
     4801 aatgatgaca atcgagaaag acgtcacatt gcgggaaatg ttcgaaatga tgggtgtcga
     4861 gcaatttaca agataccctg tcacggatgg cgacaaggac catgtcattg gcttggtcaa
     4921 tatgaaaaac ctgctgacgg ctttcatcaa agacccttct acgggagatc agcctgtcag
     4981 tgaatatatg cagccgatca tcagggtcat cgaaacgatg gcgatcggtg atctgttgtt
     5041 gaaaatccaa cgcgaacgga tccatatggc cattcttatg gatgaatacg gaggaacttc
     5101 cggactcgtg acgatcgaag atattttaga agagattgtc ggggaaatcc aggatgagtt
     5161 cgatatggac gaagtgcctg aggttcagat gatcaatgaa aaccactata ttctggacgc
     5221 gaaaatgtta attgaaaacg tgaatgacgt actcgatatc gatattgatg aagaggatat
     5281 cgacacgatc ggcggatggt tcatgtccga acgttttgaa gcggtccctg gtgaaaagat
     5341 cattgaacaa ggctatgaat tcaccgtgaa agacgtagaa ggtcatcaca tcctttactt
     5401 ggaagcgatg aaaaatgaaa aaacgaatgg aaccaatgac ttacaagttg agtcttaaca
     5461 aaagcgcagg gtgcctgcta gaggcgacag gcaaagccgt cacgaagaac ggcttttgcg
     5521 accacaagcg cagcgttggg agcaagtatt ttgaggatgt ttcttttttt ccagttcccg
     5581 gagtctagac gtaaaattac aaacggcatc tacctctatc tgaggagatg ccgcttttga
     5641 tttcactgtt ctttcttata aacgacccgg tacacatcgt gtctccgatc cttcaactgc
     5701 ttcacggttc cgtcctggcg ctggcgtcgc aagatctcga gatcgacatc cccgatcagg
     5761 accatctcca aattggcatt cgtttccccg acgattccat cccttgcaaa ttcgaaatcg
     5821 gacggagaaa atatggccga ctgcgcatat tggatatcca tattttccgt ttgcggcaaa
     5881 ttgccgaccg ttccggaaat gactgtgtag atctggtttt caatcgcccg ggcttgtgca
     5941 caataacgga cgcgcaaata tccttggcga tcttccgtac agaatggggt gaagatgata
     6001 ttcgcaccct tatcagtcgc aatccgcgca agctccggaa attcgatgtc ataacagact
     6061 tggattgcga tttttccgca gtcggtgtca aacaccttga tttcatcgcc ttggctgatc
     6121 ccccaccatt ttcgttcatt cggcgtaata tgaatcttgt attgcttctc gatcgaccca
     6181 tcccttctga aaaggtaggc gatattgtaa atctcctcat cctcttcctc gacgaaatgg
     6241 gatccgccga taatgttcac attg
//
LOCUS       DASULI010000109         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1226768,
            whole genome shotgun sequence.
ACCESSION   DASULI010000109 DASULI010000000
VERSION     DASULI010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226768"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFX34_02370"
     CDS             complement(<1..679)
                     /locus_tag="VFX34_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019715026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5563780.1"
                     /translation="MSKRDYYEVLGVPKTASKEEIRKAYRKLSKQYHPDLNKEAGAEE
                     KFKEVTEAFETLSDESKRAQYDQFGHADPNQGFGGFGGFGGGSADGFGFEDIFSTFFG
                     GATRRRDPNAPRKGDDLQYSMTIDFMEAVFGKETEVELPREETCDTCDGTGAKKGTPV
                     NTCSHCGGSGQISVTQNTPLGQMVNRRTCPHCQGTGKIIPEKCGTCHGSGRVTKRKKI
                     KVTIPAGV"
     gene            complement(887..2725)
                     /gene="dnaK"
                     /locus_tag="VFX34_02375"
     CDS             complement(887..2725)
                     /gene="dnaK"
                     /locus_tag="VFX34_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5563781.1"
                     /translation="MSKIIGIDLGTTNSVVAVYEGGEAKVIPNPEGNRTTPSVVAFKN
                     GERQVGEVAKRQSITNPNTIMSVKRHMGSDYKVKVEDKEYTPQEVSAMILQYMKGYAE
                     EYLGEKVTKAVITVPAYFNDAQRQATKDAGTIAGLEVERIINEPTAAALAYGLDKTDE
                     DETILVYDLGGGTFDVSILELGDGVFQVRATAGDNKLGGDDFDDVVIDYLVQEFRKEN
                     AIDLSKDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEISLSRAKFD
                     ELTAHLVERSMVPTRQAMKDADLSPSEIDRVILVGGSTRIPAVQEAIKKETGKEPFKG
                     VNPDEVVAMGAAVQGSILRGDVKDVVLLDVTPLSLGIETMGSVFTKLIERNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPPAPRGIPQIEVTFDIDK
                     NGIVTVKAKDLGTQKEQNITIQSSSGLSDEEIDRMVKDAEANAEEDKRRKEEADLKND
                     ADQLVFMAEKTLKDLEGKVSEDEVKKVEDAKEELKSAVEAGNLDDMRTKKQALEELVQ
                     QLSVKLYEQAAAEAQAQGGPAESGGQPQDDGVVDADFEEVDDDKKN"
     gene            complement(2766..3374)
                     /gene="grpE"
                     /locus_tag="VFX34_02380"
     CDS             complement(2766..3374)
                     /gene="grpE"
                     /locus_tag="VFX34_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5563782.1"
                     /translation="MTDKKEAAEKEEMQEIATEELEAVAVEEVSSEQQATAEEGSDSE
                     EVAEEIDEKDQRISELESKLQDEENKLLRVLADFENAKRRNALDQEALVKYKAQSLLT
                     SILPVLDNFERALAVEVKVEEAQSIMTGMEMIYRNLVEALKGEGLVEIEALDKEFDPN
                     FHQAVMTGNDPDKSSGIVLDELQKGYMLKDRVLRPTMVKVNE"
     gene            complement(3374..4420)
                     /gene="hrcA"
                     /locus_tag="VFX34_02385"
     CDS             complement(3374..4420)
                     /gene="hrcA"
                     /locus_tag="VFX34_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5563783.1"
                     /translation="MLTNRQLLILQLTVNDFIESAQPVGSRQLSKKPEAPYSPATIRN
                     DMADLEELGYLEKTHTSSGRVPSEKGYRFYVDHLLTPEKLTLADSMQLRSIFQDRVLE
                     TEELIRNSAKIISELTNYTSILLGPDMSMHSVKRFSIVPLDDTKAVAIIVTDNGHVEN
                     RVFDVPQGMLASDIEKMVNILNERLVGTPLSFLQNKLAHETKTVFEQHVHHAGELFAS
                     FQRAMTIKPEERLYFGGKMNMMKQPEFNDLQKMKMFFELMENGAPALTFFQDDSKGIH
                     VRIGSENKHNAMEDCSVITANYSAGENMAGSIAIIGPKRMDYGRVITMLDILSDNLSQ
                     ALSKMTIGMDRRND"
     gene            complement(4516..>5038)
                     /locus_tag="VFX34_02390"
     CDS             complement(4516..>5038)
                     /locus_tag="VFX34_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496923.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX5563784.1"
                     /translation="KGKLPLPGEDIEAEMFSMLIDRMAEAGRQQYEISNFALPGNESI
                     HNLIYWDNDEYAGIGAGAHGYLKGRRYANYGPLKKYMESMETGMRPVVEEHDVTDTEA
                     MEEEMFLGLRKMEGVSLHSFESKFSKPLKDVYGDVLKELNRKGLIYESEGSIKLTGKG
                     VFRGNEVFQQFLK"
BASE COUNT         1154 a         1291 c         1006 g         1587 t
ORIGIN      
        1 cgacgcctgc cgggatggtc actttgattt tcttgcgttt cgtcacgcgt ccggagccat
       61 ggcaagtacc gcatttttca ggaatgattt ttcctgtccc ttgacagtga ggacatgtcc
      121 ggcgattgac catctgacca agcggagtgt tttgcgtgac agaaatttgt ccggatccgc
      181 cacagtgaga gcatgtattg accggtgtgc cctttttggc cccggttccg tcgcatgtgt
      241 cacaagtttc ttctcttggc aactcaactt cagtttcttt accaaaaaca gcttccatga
      301 aatcgattgt catggaatac tgaaggtcat cgcctttcct aggcgcattc gggtcgcgac
      361 gtcttgtagc gcctccgaag aaagtgctga aaatatcctc gaagccgaaa ccgtctgcag
      421 aaccacctcc aaagcctccg aatcctccaa agccctggtt gggatccgca tggccgaatt
      481 ggtcgtactg cgcacgttta ctctcgtcgc tcaatgtctc aaacgcttcc gtcacttctt
      541 tgaatttctc ttctgctccc gcttccttgt tcaaatcggg atgatattgt ttcgataatt
      601 tccggtaggc tttcctgatt tcctctttac ttgcagtttt cggcacgccg agtacttcat
      661 aataatcacg cttgctcatt attccactct cccatgaatc tttgacattc gcatttcacg
      721 aacagtaatc cgtgctgtaa accgttccat ttccaagtta cgactcattg taacacgcac
      781 agaaaaaaga gacaaaactg cgtaagccgg attacaattg ctcaacaaaa gaaaaagtca
      841 aagccggaat atcggacttt gacttttccg tcaatcgttc gttcaattag tttttcttgt
      901 catcgtccac ttcttcaaag tcggcatcga caacaccatc atcttgcggt tggccgccag
      961 attctgctgg gccgccttgt gcttgggctt cagcagctgc ttgctcatac aatttgacag
     1021 acaattgttg gacaagctct tccaacgctt gcttcttcgt gcgcatatcg tccaggttgc
     1081 ctgcttcaac cgcggacttc aattcttctt ttgcatcctc gacttttttc acttcgtctt
     1141 cggatacttt gccttcaagg tctttcaatg tcttttctgc catgaagacg agttggtctg
     1201 cgtcattttt caagtccgct tcctctttgc ggcgcttatc ctcttccgca ttcgcttcag
     1261 catccttcac catgcgatcg atttcctcat cggaaaggcc ggagcttgat tgaattgtga
     1321 tgttctgctc tttttgcgtt ccaagatctt tcgcttttac agttacaata ccgtttttat
     1381 cgatatcgaa tgtaacttcg atttgcggga ttccgcgtgg tgcaggtggg atatccgtca
     1441 attggaaacg gccgagtgtt ttattgtctg cagccatcgg acgctcacct tgcaatacgt
     1501 gaatatctac agcaggttgg ttgtctgcag ccgtcgagaa tacttgtgat ttacttgtag
     1561 ggattgtcgt atttctttcg attaactttg taaacacgct tcccatcgtt tcaataccta
     1621 aagagagtgg cgtcacgtca agcaagacga catctttcac gtctccgcga agtattgaac
     1681 cttgaacagc ggcacccatt gctacaactt cgtccgggtt aacgcctttg aacggctctt
     1741 tgcccgtctc ttttttgata gcttcttgta cagccggaat acgagtggat cctccaacta
     1801 ggatgacacg atcgatttcc gaaggggaca agtcggcatc tttcattgct tgacgagttg
     1861 gaaccattga acgttctacg agatgcgcag tcaactcatc gaatttcgca cgcgacaatg
     1921 aaatttcaag gtgaagagga ccagcttctc ctgctgtgat gaacggcaat gagatttgtg
     1981 ttgaagtaac tccagaaagg tcttttttcg ctttttcagc ggcatccttc agacgttgca
     2041 ttgccatttt atcttttgaa aggtcgatcg cattttcttt acggaattcc tgtactaagt
     2101 aatcaatgac tacatcgtcg aagtcgtctc cacccaattt attgtcgcct gctgttgcgc
     2161 gaacttggaa cacaccatcg ccgagctcaa ggattgaaac gtcgaatgta ccgccgccaa
     2221 ggtcatagac aaggatggtt tcgtcttcat ccgtcttatc aagaccataa gcaagtgcag
     2281 ctgcagtcgg ttcgttaatg atcctttcga cttcaaggcc tgcaattgtg cccgcatcct
     2341 tcgtcgcttg acgctgcgca tcattgaagt acgctggaac cgtgatgaca gctttcgtca
     2401 ctttttcgcc caagtattct tccgcatatc ctttcatata ttgaaggatc attgccgata
     2461 cttcttgcgg agtatattcc ttatcttcaa ctttcacttt ataatcggat cccatatgtc
     2521 tttttacaga catgatcgta ttcggatttg taatggattg gcgctttgcc acttccccga
     2581 cttgacgctc tccgtttttg aatgcaacaa ctgacggcgt cgtacggttg ccttccggat
     2641 tcggaatgac tttcgcctct ccgccttcat aaactgccac tacagagttc gttgttccca
     2701 agtcgatacc gatgattttg ctcatttaaa ttcctcctaa ttaatgcgtc ttcaattagc
     2761 tagattcatt cgtttacttt gaccatagta ggtcgaagta cacgatcttt caacatatac
     2821 cccttttgaa gttcatcaag cacgatgccc gacgacttat caggatcatt tcctgtcatg
     2881 accgcctgat ggaaattcgg atcgaattcc ttatccaacg cttcgatttc gacaagaccc
     2941 tcgcctttta gtgcttcgac aaggttgcga taaatcattt ccattccagt catgatcgat
     3001 tgagcctctt ccactttaac ttccactgca agcgcacgtt cgaagttgtc caagacaggc
     3061 aaaatagacg tcagcaagct ttgtgcttta tacttcacaa gagcttcctg atcaagcgcg
     3121 ttccttcttt tggcattctc aaaatccgca aggacgcgca gaagtttatt ttcctcgtct
     3181 tgtaatttgg actcaagctc gctgattctc tgatccttct cgtctatctc ttcagcaact
     3241 tcttccgagt cacttccctc ttcggcagtt gcttgttgtt cgcttgaaac ttcctcaacc
     3301 gctactgctt ccaattcttc agttgcgatt tcctgcattt cttctttttc agctgcttct
     3361 tttttatccg tcactagtcg ttcctcctat ccattccgat ggtcattttc gataatgctt
     3421 gagacaaatt atcgcttaaa atatcaagca tcgtaatgac cctaccgtaa tccatacgtt
     3481 tcggtccgat gatcgctatg gagccggcca tattttcgcc ggcggagtaa ttcgcagtga
     3541 tcacactgca gtcttccatt gcattatgtt tgttttccga accgatccgt acatggatgc
     3601 cctttgagtc atcctggaaa aacgtcagtg ccggtgcacc gttttccatc aactcgaaaa
     3661 acatcttcat tttctgaagg tcattgaatt ccggctgttt catcatattc attttgccgc
     3721 cgaaatacag acgttcttca ggtttgattg tcatggccct ttgaaatgag gcgaacagtt
     3781 cccccgcatg atggacatgc tgttcaaaaa ccgttttcgt ctcgtgggca agcttgttct
     3841 gcaggaagct caatggcgtc ccgacaagcc tttcatttaa aatattgacc atcttttcaa
     3901 tatccgaagc aagcatgcct tgcggtacgt cgaagaccct attttccaca tgtccattat
     3961 cggtcacaat gatggcaacc gcttttgtgt catccagcgg aacgatggaa aaacgtttca
     4021 ctgaatgcat ggacatgtcc ggacctaata atatggacgt gtaattcgtc aattcggata
     4081 tgatttttgc ggaattcctt attagttcct ccgtctctag aacccgatcc tgaaaaatgg
     4141 aacgtagttg catgctgtcc gccaatgtca gcttttcggg tgtcaataaa tgatcgacat
     4201 agaaacggta ccctttttcg gaagggactc tgcctgatga agtatgggtc ttttcgagat
     4261 aacccagttc ctccaaatcg gccatgtcat ttcgaatggt cgcgggactg taaggggcct
     4321 ccggtttttt agataactgc cttgatccga caggttgggc ggattcgatg aaatcgttaa
     4381 ccgtcagttg caaaataagc aattgtctgt ttgtcaacat aatcatcact cctgttagca
     4441 ctctttaagg atgagtgcta atactactca taatttaaca aatcgaattg tcagtgtcaa
     4501 tgaattaacc ctcggtcatt tgagaaattg ctggaacact tcattccctc tgaaaacgcc
     4561 ttttcctgtc aattttatac taccctcaga ttcgtaaatc agtccttttc tgtttagctc
     4621 tttcagcaca tctccataca cgtctttcag tggtttgcta aatttagatt cgaatgagtg
     4681 aagggatact ccttccattt tacgcagccc taaaaacatt tcctcttcca tcgcttcagt
     4741 atctgtcacg tcatgctctt ccacgacagg acgcattccg gtttccatcg attccatgta
     4801 tttttttaat gggccataat tcgcatatct acgacctttc aagtatccat gtgcgccagc
     4861 tccgatgcct gcgtactcat cattatccca gtaaatgaga ttatggatcg attcattccc
     4921 aggcaacgca aaattactga tttcgtattg ctgtcttccc gcttcagcca tgcggtcgat
     4981 cagcatggaa aacatctccg cttcaatatc ctctccaggc agaggcagtt tcccttta
//
LOCUS       DASULI010000110         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_159598, whole
            genome shotgun sequence.
ACCESSION   DASULI010000110 DASULI010000000
VERSION     DASULI010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159598"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="secA2"
                     /locus_tag="VFX34_02395"
     CDS             complement(<1..880)
                     /gene="secA2"
                     /locus_tag="VFX34_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292886.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, especially gram positive
                     pathogens, have paralogs that have been found to be
                     nonessential but do function in secretion of a subset of
                     exported proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEX5563785.1"
                     /translation="MLSIFKRSNQTSERQLRKYRKVVRHINDLESKYETLSDEELAHM
                     TEVFKVQIQEGENVQAILPDAFAVVREASKRVLGMRHFDVQLIGGMVLAEGNIAEMPT
                     GEGKTLVASLPSYLRALEGKGVHVITVNDYLARRDYDQIGQIHRFLGLTVGLNVPMMQ
                     GPEKQDAYMADITYGVGTEFGFDYLRDNMVQHPSQKVQRPYHFAIIDEVDSVLIDEAK
                     TPLIVAGKMQADPDLHFIAARLAKRFKKGVDFEFDDETKATSLTEAGIEKVEKAFGID
                     NLYELEHQTLYHYMIQA"
     gene            1111..2154
                     /locus_tag="VFX34_02400"
     CDS             1111..2154
                     /locus_tag="VFX34_02400"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004230902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX5563786.1"
                     /translation="MLFLAMAAAFIASIILTPLVIKFAFRIGAVDHPNYRKVHASVMP
                     RIGGMAVFGAFVIGYLLLRPSDEHAVGILVGAVIIIITGFLDDMLEITAKAKLFGQLA
                     AAIVVVTWGGLQIEFINLPFFGPFDFGYLSIPITILWIIGITNAINLIDGLDGLAAGV
                     STIALISITVMAVIMGDVFVVATAAILAASTLGFLFYNFHPAKIFMGDTGALFLGYMI
                     SVLALLGFKNVAVVSLVIPIIMLGVPISDTFFAIVRRVRMKQPISAPDKSHLHHCLLR
                     AGFSHRQTVLIIYGLAILFGVAAVLFSQATVWGAIVLIAVMLIAIELFVEIIGLAGTN
                     YRPLLNLVRMIGK"
     gene            complement(2281..>2566)
                     /locus_tag="VFX34_02405"
     CDS             complement(2281..>2566)
                     /locus_tag="VFX34_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEX5563787.1"
                     /translation="GDNMKTDMTFKEMTSFWEYAKGGMPQIDTINLKGYDDWSKGPYY
                     YQLDETDLANVSQLLKAHLGLIPDSSSLTDSSGANGMGTADETAEGSDGY"
BASE COUNT          595 a          603 c          599 g          769 t
ORIGIN      
        1 cggcttggat catataatgg tagagtgtct ggtgctcaag ctcgtaaagg ttgtcgatgc
       61 cgaatgcctt ctccactttc tcgattccgg cttccgtaag tgaagtcgct tttgtttcat
      121 catcaaattc aaaatcgacg ccttttttga atcgctttgc aagtctggcg gcgatgaaat
      181 gaagatccgg atccgcctgc attttccccg cgacgatcaa cggtgttttc gcctcatcga
      241 tcaagacgct gtccacttcg tcaatgatgg cgaagtgata agggcgctgc accttttggg
      301 aaggatgctg gaccatatta tcacgcaggt aatcaaaacc gaactcggtg ccgacgccat
      361 atgtaatatc ggccatatag gcatcctgct tctcaggtcc ttgcatcatc ggtacgttca
      421 agccgaccgt caatccgagg aaacggtgga tctggccgat ttgatcatag tcgcgccttg
      481 caaggtaatc gttcactgta atgacgtgga cgccttttcc ttcaagcgcg cgcaagtagg
      541 agggaaggga agcgactagc gtctttcctt cacccgtcgg catttccgcg atattgcctt
      601 cagccaatac catgccgccg atcagctgaa cgtcaaaatg acgcatgccc aatacccgct
      661 ttgatgcttc acggacgacg gcgaatgcgt ctggaaggat ggcttgcaca ttttcgcctt
      721 cctggatctg cactttgaac acttctgtca tatgggcaag ttcttcatct gacaatgttt
      781 cgtattttga ttccaagtca tttatgtgac ggacaacttt gcggtatttg cgaagttgcc
      841 tttcgctcgt ttgatttgaa cgtttgaaaa ttgataacat ataattggcg ctcctttatc
      901 tcatccttct attgaaagga tacaagttct attttatcaa actataaacg aaaagactac
      961 cttgttaaga aattggaagg atacatcctt tgctaaacgg gtatagtcgt tcagagtgcc
     1021 cagtttcaca aacatggatg ctttccattg acagctgtgc tataatactt gtggctaact
     1081 aaattattgt catttacagg gaggaaggac atgttatttc ttgcaatggc tgctgcattc
     1141 atagcttcca tcatactgac tccacttgtc ataaaattcg cgttccgcat cggagcggtc
     1201 gatcatccta actatcggaa ggtgcatgca tccgtcatgc cgcgtatcgg ggggatggca
     1261 gttttcggcg cttttgtcat agggtatcta ttactgcgtc cttcagatga gcatgctgtc
     1321 ggaatccttg tcggagcggt tatcatcatt attacaggct tcctcgatga tatgcttgaa
     1381 atcaccgcca aggcgaagct gttcggccaa ctggccgcgg caatcgtcgt tgtgacatgg
     1441 ggcggtttac aaatcgaatt cattaacttg ccatttttcg gcccgtttga ctttggatat
     1501 ttaagtattc caatcacgat actttggatc atcggcatta caaatgctat caatctcatt
     1561 gatggtttgg atggtcttgc ggcgggcgtt tcgacaatcg cacttatttc catcacggtc
     1621 atggccgtga tcatgggcga cgtattcgtt gtagcgacag ctgccatcct ggctgcgagc
     1681 acactcggat ttctgttcta caattttcat cccgcgaaga ttttcatggg ggataccggg
     1741 gcgctgttcc ttggctacat gatttcagta ttggcgttgt taggttttaa gaatgtcgcg
     1801 gttgtgtcac ttgtgatccc gattattatg ctgggtgttc cgatttcaga tacattcttc
     1861 gcaatcgtcc gccgggtgcg gatgaagcag ccgatttcag caccggacaa atcgcacttg
     1921 caccattgtc tgctccgtgc aggcttctca catagacaga ctgtcctgat catctatgga
     1981 ctggctatcc ttttcggtgt agctgcagtg ctattttcgc aagcgactgt gtggggcgcc
     2041 atcgtcttga tcgctgtcat gcttattgca attgagctgt tcgtcgaaat catcggactt
     2101 gcaggaacga attaccggcc attattgaat ttggttcgta tgataggaaa ataaatatgc
     2161 aacagtggtg atatgcgaaa tttcgcatat cgcttttttg tatctaaaga aaaggacaac
     2221 gaaaaaaatc ccgctgaaat gaattcagcc gggatttgaa gttccattga tgtattctat
     2281 ttaataacca tcgctgcctt cagcagtttc gtctgctgtc cccatgccat tggctccgct
     2341 gctgtcggtc aacgaggacg aatcggggat caaaccgaga tgggctttca gcagctggct
     2401 cacattcgct aaatcagttt cgtccaactg ataataatag ggccctttcg accaatcatc
     2461 atagcctttc aaattaatcg tatcgatttg cggcatgccg ccttttgcat actcccagaa
     2521 ggaagtcatt tctttgaacg tcatgtcggt tttcatatta tcgccg
//
LOCUS       DASULI010000111         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_296529, whole
            genome shotgun sequence.
ACCESSION   DASULI010000111 DASULI010000000
VERSION     DASULI010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296529"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFX34_02410"
     CDS             <1..541
                     /locus_tag="VFX34_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563788.1"
                     /translation="HEKVGTYSLGMRQRLGLAQALLHRPKFLILDEPTNGLDPAGIRE
                     FRQYLRNIAETEGVSVFVSSHMLSEIELMCDRIAIIQNGKLIDIREMSEMRESFYYIE
                     ASPSEKAKQLLLDGGYTVEAFKDGMLVNAEQQQIPRLVESFIAEGLQVYAVQQHRKTL
                     EDEFLEMTGGGQIAEAYAK"
     gene            510..1286
                     /locus_tag="VFX34_02415"
     CDS             510..1286
                     /locus_tag="VFX34_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563789.1"
                     /translation="MARLLKLMQNEWMKLWHKKGTWVMVGILIVITAGMMGLMKYIST
                     QNMGGSFTKEGMIQDPSSLGGTVLLLTVIVAAGIVASEFSQGTIKMLLTRPVKRWKIL
                     LSKFLTVNLFGMLLMLIGYIVYILLAILLFKSDAGASLSSWSVWSKSLYMLLLSFGSV
                     FVTATFAFTVGSVFRSSSLAIGLSLFIYFTGSTISALLSQYEIAKYLLFTHMDLTQFE
                     TGMMLIPDLTMPFSFAVLAVYMVAFLVISFSTFIKRDVTA"
     gene            1520..2809
                     /locus_tag="VFX34_02420"
     CDS             1520..2809
                     /locus_tag="VFX34_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX5563790.1"
                     /translation="MKKPLKVLAAIAIMASMLGPAGSVSAKDDFRDFKEDQMKQRGET
                     FRVLIKATNDQELQQLQQNYELRNEFDGHSYTTDVTAKEFSALQNMPHITVEKVDVLS
                     IQIDPASLKVNDEMEAAAASQSVPWGIKAIYNDNNLTYTTGGSNVRIAVLDTGVNVNH
                     NDLYYNAEQCKDFTQSASLVNNTCTDRNGHGTHVAGTALGEGGDDRRGVYGVAPNAKL
                     WAYKVLNDSGSGYADDIANAIRHAADQAYSQQVKVVINMSLGSNGESSLITSAVNYAY
                     DRGVLIVAAAGNDGFSPGSIDYPAALPSAIAVANLENRLENGTFRVANSSSRGHTRTA
                     GDYTIQTGDVELSAPGTSIFSTWYNGGYATISGTSMATPHVAGLAAKVWAENPSFTNV
                     QLRSNLQNRAKSYDILGGYHAGSGDDIASGFGFARVR"
     gene            2942..>3336
                     /locus_tag="VFX34_02425"
     CDS             2942..>3336
                     /locus_tag="VFX34_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5563791.1"
                     /translation="MNIGSILKYYRMKNNMTQSELCKGVCSISHLSKIESNKYIPHTE
                     TINELFKRMGIEWEREVDTYAYWNEKLETFIMHSVYYDLKSMEEVYKELATQEDYLQS
                     TDLVNRYELYKLRYYLFKQEIPRATQQVS"
BASE COUNT          927 a          736 c          842 g          831 t
ORIGIN      
        1 ccatgagaaa gtagggacgt attcattggg gatgcgccag cgtctcggtt tggcacaggc
       61 actcctgcat cgcccgaaat tcttgatttt ggatgaaccg acaaatggac tggatcctgc
      121 gggaatccgc gaattccgcc aatatttgcg taatattgcc gagacggaag gggtttccgt
      181 tttcgtttca agccatatgc tatccgaaat cgagctcatg tgcgatcgga ttgccatcat
      241 tcaaaatggc aagctcatcg acatccgtga aatgtccgag atgcgggaat cgttctatta
      301 tatcgaagct tcaccatcgg aaaaggcgaa gcaactgctt ctcgacggag gatatacggt
      361 ggaagcgttt aaagacggaa tgctcgtcaa tgcggagcag cagcaaattc cgcgcttggt
      421 cgagtcgttc attgccgaag ggctgcaagt gtatgcggtt cagcagcacc ggaagacgtt
      481 ggaagatgag ttcctagaga tgactggagg tggccagatt gctgaagctt atgcaaaatg
      541 aatggatgaa attgtggcat aaaaaaggga cgtgggtcat ggtcggcatt ctcattgtga
      601 ttacggcggg catgatgggg ctgatgaaat atattagtac gcagaatatg ggcggcagtt
      661 ttacgaagga agggatgatc caggatccgt cttcacttgg cggaaccgtc ctattgctga
      721 ccgtcatcgt tgcagcgggt attgttgcgt ccgagttctc gcaggggacg atcaagatgc
      781 tgctgacacg cccggtgaaa cgttggaaga ttcttttgtc caaattcctt acagtgaatc
      841 tgtttggcat gcttcttatg ctgatcggct atattgtcta tatccttttg gcgattctat
      901 tgttcaaatc ggatgcgggc gctagtctgt caagttggtc ggtttggagt aaaagtctgt
      961 atatgctgct cctatcattc ggcagcgtat tcgtcacagc gaccttcgcg ttcacagtcg
     1021 gcagtgtctt ccggtcaagc tcgcttgcaa tcggcttgtc gctgttcatt tatttcaccg
     1081 gctcgacgat cagcgcgctg ctttcgcaat atgaaatcgc gaagtacttg cttttcacac
     1141 atatggattt gactcaattc gaaacaggca tgatgttaat ccctgactta acgatgccat
     1201 tttcattcgc cgtattggct gtttacatgg tcgctttcct cgtcatcagc ttttcgacgt
     1261 tcataaaacg agatgttacg gcttgattgg taggtataat cagataggaa atgcccgatt
     1321 tgcccaacac cggctgtagt cagagccggt gttttctagt ccattcgaat caaaaaactg
     1381 acagaacagg ggtggataga tagagaagga agcgcgtaaa ctgtcgaatc ctatcagatt
     1441 tttcccaatt tcctatagtc ggaatgtttg ctacagtgaa ggtagcaaca ccgaatctat
     1501 taggaaaaga ggagaatgaa tgaagaaacc attgaaggtg ctcgcagcaa ttgcaattat
     1561 ggcaagtatg ctcggtccag cgggaagcgt atcggcaaag gacgatttcc gggattttaa
     1621 agaggatcag atgaagcaaa ggggcgaaac gttccgcgtg ctcatcaagg caacgaatga
     1681 ccaggaattg caacagcttc aacaaaacta tgagctgcgc aatgaattcg acggccattc
     1741 ctacacgacg gacgtgacag cgaaagaatt ctcggctttg caaaatatgc cgcatatcac
     1801 tgtcgaaaaa gtggacgtcc tttccatcca gatcgatcca gcatccttga aggtgaatga
     1861 tgaaatggaa gcagcagctg ccagccagtc cgttccttgg ggaatcaaag cgatctataa
     1921 cgacaataat ttaacgtata cgacaggcgg ctcgaacgta agaatcgccg ttcttgatac
     1981 aggtgtgaat gtgaatcata acgatttata ttacaacgcg gaacaatgca aggatttcac
     2041 gcaaagcgca tcgcttgtga ataatacgtg taccgaccga aacgggcacg gaacccacgt
     2101 tgcaggaact gccttaggcg agggcggcga tgaccgtaga ggcgtttacg gagtggcccc
     2161 gaatgcgaag ctttgggcgt ataaagtatt gaacgactcc ggaagcggct atgcggatga
     2221 tatcgcgaat gcaattcgac acgcggcaga tcaagcatat tcccaacaag tgaaagtcgt
     2281 catcaacatg tcccttggtt cgaacggaga aagctcgtta atcacgagtg ctgtcaatta
     2341 cgcttatgat cgtggcgtct tgattgtggc agcagcagga aacgatggct tttcacctgg
     2401 ctcgatcgat tacccggcag ctttgccatc tgcaattgct gtcgctaacc ttgaaaatcg
     2461 gttggaaaac ggcacattcc gtgtcgccaa ctcttcttcg cgtggccata cgagaacggc
     2521 cggcgactat acgatccaaa caggagatgt tgaattgtca gcacctggta catccatttt
     2581 ctcgacttgg tacaacggcg ggtacgcgac aatcagcggt acatccatgg caacaccgca
     2641 tgtggcagga ttggcggcga aagtatgggc ggaaaaccca agcttcacta acgttcagct
     2701 tcgctccaac ctgcaaaatc gtgcgaaatc atatgatatc ctcggcggtt atcatgccgg
     2761 aagcggagac gacattgcat ccggattcgg attcgcaaga gtacgttaac atagcctcct
     2821 aatgaaaatc ccatacatta ggccgaaaac agtcccttac tcccctggga ctgttttctc
     2881 atgtattcaa ttaatggaaa tgatatgttt tgaatagtaa gaaatcaaag gggtgctaat
     2941 gatgaatatc ggatccatcc taaaatacta cagaatgaaa aataatatga cacaaagcga
     3001 gctatgcaaa ggagtctgct ccatttccca tttaagcaaa atcgagtcga ataaatatat
     3061 tccccataca gaaacgatca atgaactgtt caagcggatg ggcatcgaat gggagaggga
     3121 agtggataca tatgcatact ggaatgagaa attggaaacg ttcatcatgc actcagtcta
     3181 ctacgaccta aaatcaatgg aggaggtata caaagagctt gccacgcaag aagactattt
     3241 gcaatcaacg gatttggtca atcggtatga gctctataaa ttgcgttatt atttattcaa
     3301 acaggaaatt ccccgcgcca cccagcaagt ttccat
//
LOCUS       DASULI010000112         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1384082,
            whole genome shotgun sequence.
ACCESSION   DASULI010000112 DASULI010000000
VERSION     DASULI010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384082"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFX34_02430"
     CDS             <1..199
                     /locus_tag="VFX34_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426793.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEX5563792.1"
                     /translation="GIVLDDEKVISSSNRGEPVVMDPSNPAAIGYRNIARRILGESVP
                     LMSLDEGKPGFFGKLKSLFAK"
     gene            337..888
                     /locus_tag="VFX34_02435"
     CDS             337..888
                     /locus_tag="VFX34_02435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5563793.1"
                     /translation="MKWKTKWVIAIILAIGVFSFAKLEEIGIINKPVTQYITTGKDFI
                     VMKKWVASIVGDSDEDTITVTANPEEEIPFAAYESMQPYKDGVIASYAHPMPITAQGD
                     GLIVFTGFTRQSGKTITVLYDDGDEVTYGFVGSISKLPYSTVKKGDTLALMNEEAIYL
                     KVKREGVVLEAAVLATYFSEDTK"
     gene            885..1496
                     /locus_tag="VFX34_02440"
     CDS             885..1496
                     /locus_tag="VFX34_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5563794.1"
                     /translation="MKLRLHPILAPFFLVLMITGGMAFYALVFISLLFHEAGHIFAAR
                     MMGMKVRSCTIMPYGGELRIPDRNSYGKRQRVFVAIGGPAATAILLLSTLIEFPGNEQ
                     FMRIQLVILSLNLLPILPLDGGQAISAMLETEEKKYKVRSSFLLFSIAVLSAVCLLLL
                     EGLPETVLFLALSIFLLIQNISAFRFRKYEKAYEQLKRKQLTP"
     misc_feature    1538..1611
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1625..1933
                     /gene="rplU"
                     /locus_tag="VFX34_02445"
     CDS             1625..1933
                     /gene="rplU"
                     /locus_tag="VFX34_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5563795.1"
                     /translation="MYAIIETGGKQIKVEQGQEVYIEKVVGEAEETVTFDKVLFVGGE
                     DVKVGAPFVEGATVTGKIVKHGRGKKITVFKYKPKKNYHKKQGHRQPYTKVVIEGINL
                     "
     gene            1940..2266
                     /locus_tag="VFX34_02450"
     CDS             1940..2266
                     /locus_tag="VFX34_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEX5563796.1"
                     /translation="MIKISIDEQPSGRIDSFEMKGHADFAEHGKDLVCAGASAVSFGA
                     VNAIIALTGITPHIQQGSDGGYLKVVFPETEKDEEIQLIVKAMIVSLQTIEQDYGQHI
                     KLIFNR"
     gene            2279..2569
                     /gene="rpmA"
                     /locus_tag="VFX34_02455"
     CDS             2279..2569
                     /gene="rpmA"
                     /locus_tag="VFX34_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5563797.1"
                     /translation="MLRLDLQFFASKKGVGSTKNGRDSESKRLGAKRADGQFVSGGSI
                     LYRQRGTKIHPGENVGRGGDDTLFAKVDGVVRFERFGRDKKKVSVYPVAQEA"
     gene            2791..3309
                     /locus_tag="VFX34_02460"
     CDS             2791..3309
                     /locus_tag="VFX34_02460"
                     /inference="COORDINATES: protein motif:HMM:NF026040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEX5563798.1"
                     /translation="MDSENLTIGKALKFARHDFLNDLQLMLLYMDLGKMPEARRTLLE
                     ATERMRHLSMLEKLRMPKTEIWLSTFEWRHTVFAKKLQCNIIAGNRTAQDETIADYLE
                     KLIGIVEEAVDPMEEYIVHIVVKAAEEKWSIQLTIEGHLNGTPELPQTDGGFEVIGVL
                     QDKEWTFTISGR"
     gene            3321..>3565
                     /locus_tag="VFX34_02465"
     CDS             3321..>3565
                     /locus_tag="VFX34_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX5563799.1"
                     /translation="MFVDHVKVYVKGGDGGDGMVAFRREKYVAYGGPAGGDGGKGGDV
                     VFIVDEGLRTLMDFRYQRHFKATRGEHGGSKNRHGKGA"
BASE COUNT         1016 a          732 c          901 g          916 t
ORIGIN      
        1 tggaatcgta ttggatgatg agaaagtcat atcctcttcg aatagagggg agcctgttgt
       61 catggatcct tcgaatcctg cagcgatcgg ctaccggaac attgctcggc gtatcttggg
      121 cgaatcggta ccgctcatgt cactggacga aggaaaaccc ggattctttg gtaaactgaa
      181 atcattattc gcaaaataac tagagccctt gtctaatcag gggttctttt tttgcgacaa
      241 tattaccttg tgcataataa agagtatact tggacatttg cccataatat tcatagtaca
      301 cccgtcatat actgagaaaa aaggtggtgt ggcggggtga aatggaaaac aaaatgggtc
      361 attgcaatca tactagcgat tggtgtattc agctttgcca aattggagga aatcggaatc
      421 atcaacaagc ctgttactca atatattacg acaggcaaag atttcattgt catgaagaag
      481 tgggtcgcct ccatcgttgg agattctgac gaggatacga tcacagttac tgccaatcct
      541 gaagaggaaa ttccctttgc ggcctatgaa tcgatgcagc cctataaaga tggcgtaatc
      601 gcttcatatg cccatccgat gccgattaca gcacaagggg acgggcttat cgtcttcacc
      661 ggttttaccc ggcaatcagg aaagacgatc accgttttgt acgatgatgg ggacgaagtg
      721 acatacggat ttgtgggatc gatctcgaag cttccttatt caactgtgaa aaagggggac
      781 acgcttgccc tcatgaatga agaggcgatt tacctgaaag tgaaacggga aggggtcgtg
      841 ttggaagctg cggttttggc tacttatttc tcggaagata cgaaatgaag cttcgcctcc
      901 atcccatttt ggcacctttt ttccttgtgc tgatgattac tggaggaatg gcgttttatg
      961 cgcttgtctt catttcactt ctcttccatg aggcgggcca tatttttgct gcgagaatga
     1021 tgggcatgaa agtcaggtcg tgcacaatca tgccttatgg aggagaactg cggatacccg
     1081 accgaaattc ttacgggaaa aggcaaagag tgttcgttgc gatcggcgga cctgccgcga
     1141 cagcaattct tctcctatcg acacttattg aatttcctgg aaatgaacaa ttcatgcgca
     1201 ttcaattagt gatcctgtca ctgaacttat tgcctatttt gccgcttgac ggtggacagg
     1261 ccatcagcgc catgcttgaa acggaagaaa agaaatacaa agtgagatca tcatttctcc
     1321 tcttttccat agcggttttg tcagctgtct gcctattgct tcttgaggga cttccggaga
     1381 ctgtcctttt ccttgcgctt tccatatttt tattgattca aaacatttcc gctttccggt
     1441 tcaggaaata tgaaaaggca tatgagcaat taaaaagaaa acagttgacg ccatgacctg
     1501 cgcgtggtag tattttaatg ttattgtttg tagcgcacct gtgctacaac cgcgctgaca
     1561 aggtacaagc atccggaagg atcacctttg aaggcgagtc tgagtctatt gaggaggtgc
     1621 aagtatgtac gcaatcattg aaactggtgg aaaacaaatc aaagtcgaac aaggccaaga
     1681 agtctacatc gaaaaagtag tcggcgaagc tgaagaaact gtaacattcg acaaagttct
     1741 attcgttggt ggagaagacg tgaaagtcgg cgctccattc gtggaaggtg caacggttac
     1801 tggtaaaatc gttaagcatg gccgcggcaa gaaaatcacg gttttcaaat acaagccgaa
     1861 aaagaactac cacaaaaagc aaggtcatcg tcagccatac acaaaagttg tcatcgaagg
     1921 catcaatctg taagccggca tgatcaaaat cagtattgat gaacaaccat ccggccgcat
     1981 cgactctttt gagatgaaag gccatgccga tttcgcagaa catggaaaag acctcgtctg
     2041 tgcgggcgca tccgcggttt cattcggagc cgtcaatgcc atcatcgcac tcactggcat
     2101 aacccctcat atccagcaag gatccgacgg aggttatttg aaagtggttt ttccggaaac
     2161 ggagaaggac gaagaaatcc aattgatcgt gaaggcgatg attgtttcat tacagacgat
     2221 tgaacaagat tacgggcaac atataaaact tattttcaac cgataggagg tggcactgat
     2281 gttacgtttg gatctccagt ttttcgcatc gaaaaaaggg gtaggttcta caaagaacgg
     2341 ccgtgactct gaatcgaaac gtcttggcgc taaacgtgct gatggacaat tcgtttccgg
     2401 cggctctatc ctttatcgcc aacgcggaac aaaaattcac ccaggtgaaa acgttggccg
     2461 cggcggtgac gatactcttt tcgcaaaagt tgacggcgta gttcgttttg aacgctttgg
     2521 ccgcgacaag aaaaaagtta gcgtataccc tgtagctcaa gaagcttaac tgaattcatt
     2581 cagttaagcc tccggcggat gtcacagatt tttaaatgaa tttatcgagc aagctcgata
     2641 aaaatctgga cgcacttacg ccgaggcgta agtgattgtt atcactaaca ttggaaggac
     2701 tgcattttat tgtgcagtcc ttttttattt tcgtgaattt ttcgggttgg gtgttatact
     2761 gtagtatatg aaacagttgg cggtgagaaa atggattcgg aaaacctgac gattggaaag
     2821 gcactgaagt ttgcgcgcca tgatttttta aatgacctgc aattaatgtt attatatatg
     2881 gatctcggga aaatgcccga agcgcgaaga acactgttgg aagcgacgga acgcatgcgt
     2941 cacttgtcca tgctggaaaa gctccgcatg ccgaagacgg aaatctggct cagtacgttc
     3001 gaatggagac atactgtgtt tgcgaaaaag cttcaatgca acatcattgc cgggaaccgc
     3061 acagcacaag acgagacgat tgccgattac ctggaaaaat tgatcggcat tgtcgaagaa
     3121 gcggtcgatc cgatggagga atacatcgtt catatagttg tgaaagctgc tgaagagaaa
     3181 tggtcaattc aattgacaat cgaagggcac ctcaatggaa caccggaatt gccacaaacg
     3241 gatggcggat ttgaagtgat tggagttctg caggataagg aatggacgtt cacaattagt
     3301 ggacgatagg aggaaaaaac atgtttgtcg atcacgttaa agtatatgta aaaggcggcg
     3361 acggcggcga tggaatggtt gcattccgcc gcgagaagta tgttgcatac ggaggtccgg
     3421 ccggaggaga tggcggaaaa ggcggagatg tcgttttcat cgtcgatgaa ggtttgagga
     3481 cgcttatgga tttccgttac caacgccatt tcaaagctac tcgcggagag catggcggca
     3541 gtaaaaatag gcacggcaaa ggcgc
//
LOCUS       DASULI010000113         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1872106,
            whole genome shotgun sequence.
ACCESSION   DASULI010000113 DASULI010000000
VERSION     DASULI010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872106"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /gene="modA"
                     /locus_tag="VFX34_02470"
     CDS             <1..320
                     /gene="modA"
                     /locus_tag="VFX34_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5563800.1"
                     /translation="YAKEVLEHLNLWKPLQDKLVMGSDVRQVLTYVEMGNAEYGIVYS
                     TDAFASDKVVVLAEASPSWHTPIVYPGAVVKDSRHHDEAQDFLDFLSGEEGKAILQKY
                     GFK"
     gene            343..1038
                     /gene="modB"
                     /locus_tag="VFX34_02475"
     CDS             343..1038
                     /gene="modB"
                     /locus_tag="VFX34_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX5563801.1"
                     /translation="MTDIDYSPLLLSLKVAAISTFIVFILGVFFARIFSRKQFFGKTV
                     IESVFMLPLVLPPTVVGFALLLAFGKHGWIGKWLMDWFGVQIVFTWLGAVIASIVVSF
                     PLMYQSAAAAFDSLDEKLENAARTLGASEWRVFWTIAFPLAWPGLLAGLVLAFARGLG
                     EFGATLMIAGYIPGKTDTIPMAIYFAVESGHMEKATFWVVIIVALGFSTIMWLNWWSR
                     RNMRRYTDQNRNR"
     gene            1043..1681
                     /locus_tag="VFX34_02480"
     CDS             1043..1681
                     /locus_tag="VFX34_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563802.1"
                     /translation="MLEVNIKKRLSHYELDVDFSVGDEILVLFGPSGSGKTTILHMIA
                     GLMQPDEGQIKLRHEHYYNGENSNMPVQDRNVGMLFQDYALFPHMTAEKNIQYGMKHK
                     SGDSLLMIDQLLQVLGIQKLLTKYPHEISGGEKQRVALARALATEPAILLLDEPLSAL
                     DKQTRIDCQNELLRLHDMWKIPFIIVTHDMDEAEKLGDRILFLDHGKIVDKK"
     gene            complement(1750..2358)
                     /locus_tag="VFX34_02485"
     CDS             complement(1750..2358)
                     /locus_tag="VFX34_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5563803.1"
                     /translation="MSIIGIYLAAGKSRRMGQNKLALPVGKMALGSLALDTAVQSKLD
                     GICVVTNADDDLSWLPDQLKSHEKCHVVTCLDADDGQSASLRCGIEYAVEAGASAIVI
                     LLADQPFLTIRMIDEMVLCLNRHPDSKYIATAHDGTMKPPILFTRELFPALLTIQGDQ
                     GARNLLRHVLIDEGKQLPCEDARLVFDVDDANDYASLLSLLE"
     gene            complement(2334..3344)
                     /locus_tag="VFX34_02490"
     CDS             complement(2334..3344)
                     /locus_tag="VFX34_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5563804.1"
                     /translation="MGSIPKLVDILFENGKAAVLASVIHVEGSAYRKEGAWMFFFEDG
                     GKIGLLSGGCLEYDLQCRANEMFHTGDVKICEFDLSAEDDLGWGRGAGCNGIITVMLR
                     AVDDVFKKSLRFLQEQLKRGNPVHFLQSLESGFEFCFQSGLERYGTLMETYSMDHLKP
                     FQHHAGRKWMNDASYYHQWIWPPPSVYLFGAGADARPFAALASSVGYDVHICDWREAN
                     CTKENFPSAASFHVGPIHELLERITFTELDSVVIMTHDFRADQQILLTLQKEKLLYVG
                     LLGSVKRTERLLGRDTPEWLHSPIGLPIGADGPEEIAVSVIAEMIAIRKRRAACPSLA
                     SI"
     gene            3496..>3715
                     /locus_tag="VFX34_02495"
     CDS             3496..>3715
                     /locus_tag="VFX34_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563805.1"
                     /translation="MQFGNRTIVGFAEQRIDAVEKSTGNVKYLGDKFFQGMLHAKVAT
                     STEAHARIKSIDTSEAWKVPGVRAIVTGD"
BASE COUNT          980 a          857 c          909 g          969 t
ORIGIN      
        1 gatatgcgaa ggaagttttg gagcatctga atttatggaa gccattgcaa gacaagcttg
       61 tcatgggatc ggacgtcaga caagtgttga cctatgttga aatgggaaat gcggagtatg
      121 gcatcgtgta ttcgacagat gcatttgcat cggataaagt ggttgttttg gcagaggcaa
      181 gcccgtcttg gcatacaccg atcgtctatc cgggggccgt cgtgaaagac tccagacatc
      241 atgacgaagc acaggatttt ctggatttct tgtcgggcga agagggaaaa gcgattcttc
      301 agaagtacgg attcaagtaa gggatgaaag aaggggaaag tattgacaga cattgattat
      361 tcacctttac tgctttcctt gaaagtggct gccatttcga cgtttatcgt attcattcta
      421 ggcgtctttt tcgcacgtat tttttccagg aaacaatttt ttggcaagac ggtaattgag
      481 tctgtcttca tgcttcctct cgttttgcca cctactgtag tcggttttgc actgttgctc
      541 gcttttggaa agcatggttg gattggcaaa tggctgatgg attggttcgg tgtccagatt
      601 gtgttcacat ggcttggcgc ggttatcgct tccattgtcg tctcattccc tttgatgtat
      661 caaagtgcag cggctgcatt cgacagcctc gatgagaagc ttgaaaatgc agcacggaca
      721 ttaggagcat ccgaatggcg tgtattttgg acaatcgctt tcccgcttgc atggccagga
      781 ttgttagcag ggctcgtcct tgcatttgca cggggactcg gcgaattcgg tgccactcta
      841 atgattgccg gctacattcc agggaagacg gatacgattc caatggcaat ctactttgcg
      901 gtggaatcgg gtcatatgga gaaggcgacg ttctgggttg tcatcattgt cgctttaggt
      961 tttagcacta tcatgtggct caactggtgg agccgccgga atatgagacg ctatacggat
     1021 caaaatcgga ataggtgatc gaatgcttga agtgaatatt aaaaaacgtt tatcccacta
     1081 tgaattggac gtcgatttca gcgtgggaga tgaaatcctt gttcttttcg gtccatcggg
     1141 ctccggaaaa acaacgattc tacatatgat tgcagggttg atgcaacctg acgaagggca
     1201 aatcaaatta aggcacgagc attactacaa tggtgaaaac agcaatatgc cagtgcagga
     1261 tcgcaacgtt ggaatgctgt tccaagacta tgcgttgttc ccgcatatga cagcggagaa
     1321 gaacatccaa tatggcatga aacataaaag tggggacagc ttgcttatga tcgatcagct
     1381 gctgcaagtt cttggcatcc aaaaactatt gacaaaatac ccccatgaaa tatccggcgg
     1441 cgaaaagcaa cgtgtcgctc tcgcacgagc attggcgacg gaacctgcca tcctgctgtt
     1501 ggatgagccg ctttctgcac tggataagca gacacgtatc gattgccaaa acgaattgct
     1561 tcgcttgcat gacatgtgga aaatcccatt catcatcgtt acacatgata tggatgaagc
     1621 cgaaaaacta ggagatcgga ttttatttct cgatcatgga aagattgttg acaaaaaata
     1681 gataagatac cctataggaa tgccctgcga ctctaatcgg tgagcgcagg gcatttttag
     1741 ttccgttttt tattccaaaa gggaaagcaa ggatgcataa tcatttgcgt catccacatc
     1801 aaatacgagt ctcgcatcct cacatggaag ttgtttacct tcatcgatca aaacgtgccg
     1861 caataaattc cttgcgcctt gatctccctg aatagtcaaa agggcgggga acagctcacg
     1921 tgtaaacagg attgggggtt tcatcgttcc atcatgggca gtagcaatat atttactgtc
     1981 aggatgccgg ttcagacata ataccatttc atcgatcatg cggatcgtaa ggaaaggctg
     2041 atccgccaat aggattacga tagcggaagc gccggcttca actgcatatt ctataccgca
     2101 gcgtagagag gcggactgtc catcatcggc atccaagcat gtgacaacgt ggcatttctc
     2161 atgcgacttc aactgatcag gcagccagga caggtcgtcg tccgcattcg taacgacgca
     2221 gattccatct agcttggatt ggacggcagt gtctagcgcc aggcttccta aagccatctt
     2281 gccgacggga agcgccagtt tgttttggcc catcctccga cttttccctg cagctaaata
     2341 gatgccaatg atggacatgc ggctctcctt ttccggatgg cgatcatttc ggctatgacg
     2401 ctgacggcaa tttcttcagg accatcggca ccgattggca gaccgatagg ggaatggagc
     2461 cattcgggcg tgtcccggcc tagcaaacgt tccgttcttt tcaccgaacc caataaaccg
     2521 acatacaata gcttttcttt ttgtaaagta agcaggattt gctgatcggc ccggaaatca
     2581 tgcgtcatga tgacgactga atcaagttct gtaaacgtaa tccgttccag caactcatga
     2641 ataggaccga cgtggaaaga ggctgcggac ggaaaattct ctttcgtgca atttgcctca
     2701 cgccaatcgc atatatgaac gtcataacca accgaagatg ccaatgctgc gaacggtcga
     2761 gcgtcagcac ctgctccgaa cagatataca gatggaggcg gccaaatcca ttgatgatag
     2821 tagctcgcat cattcatcca tttccgtcca gcatgatgct gaaacggctt caaatggtcc
     2881 attgaatagg tttccatcaa tgttccataa cgctccaggc cgctttggaa gcagaattca
     2941 aatccgcttt ccaatgactg cagaaaatgg actggatttc cacgcttaag ttgttcttgc
     3001 aggaatcgca gcgacttttt gaagacgtca tcgacagcgc gaagcatcac ggttatgatg
     3061 ccgttgcatc ctgcgccacg gccccatccc aagtcatctt ccgcgctcag atcaaattcg
     3121 caaatcttca cgtctccggt atggaacatc tcattagccc tgcattgcag atcatattcc
     3181 agacagccgc cgctaagcaa tccgattttg ccgccatcct cgaaaaagaa catccatgcg
     3241 ccttccttcc ggtaagcgga tccctctacg tggataacgc ttgccaggac ggcagctttt
     3301 ccgttttcaa aaagtatgtc tactaacttc ggaatggaac ccatcaatca tcacctctat
     3361 ttttcattca ataggcccag tctcatcaac ttgatacgta tttattcggg tgaaccttat
     3421 aaatgcggcc cgaactggct aacctgttta acagctgggg ttaaataggt ttagtcagta
     3481 agggaggaag aaatcttgca gtttggaaat cgaacgatcg tcgggtttgc ggaacaacgt
     3541 attgacgccg tcgagaaatc caccggaaat gtcaaatacc tcggagataa attttttcaa
     3601 ggaatgcttc atgcgaaagt ggcaacgagt acggaggcgc atgcccgcat caaatctatt
     3661 gatacgagcg aggcatggaa agtgccaggt gtgcgggcaa ttgtcaccgg tgata
//
LOCUS       DASULI010000114         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_100167, whole
            genome shotgun sequence.
ACCESSION   DASULI010000114 DASULI010000000
VERSION     DASULI010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100167"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..280)
                     /locus_tag="VFX34_02500"
     CDS             complement(38..280)
                     /locus_tag="VFX34_02500"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5563806.1"
                     /translation="MSEETLRFEEAMSKLEGIVQKLESGDVPLEDAITLYKQGMELSA
                     YCHGKLQDAEKQLISIIDTNGQATPFDPSKGGDSNG"
     gene            complement(281..1636)
                     /gene="xseA"
                     /locus_tag="VFX34_02505"
     CDS             complement(281..1636)
                     /gene="xseA"
                     /locus_tag="VFX34_02505"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5563807.1"
                     /translation="MSENTYLSVHSLTKYIKRKFEADPYLRNVFVKGELSNVKIHPSG
                     HIYFTLKDDKSRIQSAMFRSAAGSLKFKPEEGMNVLITGDVNVYESSGQYQLYVQSMQ
                     PDGVGALYLAYEQLKKELAKEGLFDARWKQQLPVLPNKIGVITAQSGAAIRDICSTLQ
                     RRYPLADICLFPAIVQGPQAAPSIVRALELAESHGSIDVLILGRGGGSIEDLWAFNEE
                     AVARAIFSCRIPIISAVGHETDTTIADFVADMRAPTPTAAAEIAVPSKEELFERILER
                     KRSIYVSFSNQIKHERKRLTTYQQSYPLQFPERLYRPFTEKLSGLDERLLRSRNELTA
                     NRKADHHRLDRMLSFYSPMQRIKEEDRNLSLLKERMTRATQNELRNKNDRFGSAIRML
                     KALNPLEVMERGFSIVYKEGNVMNSVNGLGIGDTLKVQLQDGTVETEIKSITLKDEEE
                     N"
     gene            complement(1708..>2154)
                     /locus_tag="VFX34_02510"
     CDS             complement(1708..>2154)
                     /locus_tag="VFX34_02510"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823479.1"
                     /note="catalyzes the formation of
                     5,10-methenyltetrahydrofolate from
                     5,10-methylenetetrahydrofolate and subsequent formation of
                     10-formyltetrahydrofolate from
                     5,10-methenyltetrahydrofolate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEX5563808.1"
                     /translation="HGIMEMLKRSGIEIAGKHAVIIGRSNIVGKPMGQLLLQKDATVT
                     YCHSRTKHLPSFTRQADILIVAIGRAKFVTDEHIKDGAVVIDVGMNRDENGKLCGDVD
                     FEKAKKAASAITPVPGGVGPMTITMLLKNTLQSAEMACGKGSHQAK"
BASE COUNT          522 a          560 c          435 g          637 t
ORIGIN      
        1 taacggcact ttttcagaga taaaagcctg caatcgctta tccattcgaa tcgccgcctt
       61 tggaagggtc aaacggcgtc gcctgcccat tggtatctat aattgaaatc agctgttttt
      121 ccgcatcctg tagtttccca tgacaataag cggaaagctc cattccttgt ttgtataatg
      181 tgatggcgtc ctctaaaggg acgtcacccg attccagttt ttggacaatg ccttccaact
      241 tcgacattgc ttcttcaaat cgaagtgttt cctcgctcat ttagttttcc tcctcatcct
      301 tcagcgtaat cgacttgatt tccgtctcca cagtcccgtc ctgcagttgg acctttaatg
      361 tgtcgccaat cccaaggccg ttgacggaat tcatgacatt tccttcttta tagacaattg
      421 aaaatccgcg ctccatcact tccaatggat ttagcgcttt aagcatcctg attgctgaac
      481 caaatcgatc gtttttgttt cgcaattcat tttgcgttgc ccttgtcatt ctttctttca
      541 gtaaagaaag atttcggtct tcctctttta tccgctgcat cggggaataa aaagatagca
      601 tcctgtccaa acggtgatga tccgcttttc tgtttgctgt cagctcattc ctgctcctta
      661 acagtcgctc atccaatcca ctcagctttt cagtgaaggg gcggtaaagc ctttcaggga
      721 attgcagcgg ataggattgc tgataggttg tcagacgttt gcgttcatgt ttgatttgat
      781 tcgaaaacga gacatagatc gagcgcttcc gttccaatat cctctcgaac agttcctcct
      841 tggaaggcac cgcgatttcc gcggcggctg tcggtgtcgg agccctcata tctgctacaa
      901 aatcggcaat tgtcgtatcc gtctcatggc cgacagcact aataattgga atcctgcaag
      961 aaaaaatcgc cctcgcaacc gcctcttcat taaaagccca aagatcttct atggaaccgc
     1021 ctccacgtcc aaggatcaat acatcgatcg agccatgtga ttcagcaagc tccaatgccc
     1081 ggacaatgga aggtgccgcc tgaggtcctt gcacgattgc cgggaacagg caaatatcgg
     1141 ccaacgggta ccgccgttgc agcgtggaac agatatccct gatggcagct cccgactggg
     1201 ctgtgatgac cccgatctta ttaggcaaaa cgggcagctg ctgtttccaa cgcgcatcaa
     1261 acagtccttc tttcgcaagt tcctttttca attgttcata agcaagatat aaagctccca
     1321 caccgtccgg ttgcattgat tggacataga gctgatattg gccgcttgat tcgtaaacat
     1381 tcacatcgcc ggtgataagg acattcatcc cttcttccgg cttgaatttc aatgagcctg
     1441 ctgctgaacg gaacatggcg gactgtatcc tgcttttatc atcctttagc gtaaaataaa
     1501 tatgtccgct cgggtggatt ttcacattgg aaagttcacc ttttacaaac acatttctta
     1561 aatatggatc cgcttcgaat ttacgtttta tgtattttgt caatgaatgc acggaaagat
     1621 acgtattttc actcaaaaac ttcacccccg ggtttccgac gtatagaaac gctgtctttc
     1681 ctttgaaaaa cagcgccatt ctttttttta tttagcctgg tggctgcctt ttccgcaagc
     1741 catttcagcg ctttgcaatg tattcttcag aagcatcgta atggtcatcg gacctacacc
     1801 gccaggaaca ggtgtaattg ccgaagctgc tttcttcgcc ttctcaaaat caacatcacc
     1861 gcataatttg ccattttcat cccggttcat accgacatca atgacaactg caccatcctt
     1921 aatatgttca tccgtcacga atttcgcacg gccgatcgca acgatcagaa tatccgcctg
     1981 tctagtgaat gatggaaggt gcttcgttct tgaatggcaa tacgttacag tagcatcttt
     2041 ttgaagcagc aattggccca taggtttgcc gacaatgttg ctccttccga taatgacagc
     2101 atgcttccct gcaatttcaa tgccggaacg cttaagcatc tccataattc catg
//
LOCUS       DASULI010000115         4499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_428151, whole
            genome shotgun sequence.
ACCESSION   DASULI010000115 DASULI010000000
VERSION     DASULI010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428151"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1896
                     /locus_tag="VFX34_02515"
     CDS             253..1896
                     /locus_tag="VFX34_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5563809.1"
                     /translation="MSLLSKTVGEVLKDWAKALPKHEAYVYPEHGIRRTYEEFDQETD
                     ELAKAFIGMGIQRGEHIAIWSDNKPQWLLSQFATGKMGAVLVTVNTNYQANELEYLLK
                     QSDATTLILDEGFKGTCYIDIIRSICPELMDSKGGVVSSEKLPELKRIILMSDREESG
                     IYKWSEFLAHADGITDEELAEREKSLTPDDVINIQYTSGTTGFPKGVMLTHHNIVNNA
                     KIIGDFMKLTEKDRLCIPVPFFHCFGAVLSNLAAVTHGSTMVILEQFDPLRVLEAVQE
                     EKCTALNGVPTMFIAELNHPDFKKFDTSTLRTGIMAGSTCPIEVMKQVIHDMGADEIT
                     ICYGQTEASPVITQTQTDDPIEKRVSTVGKPHPYVEVKIIDPLTGDEMPVGSPGELCT
                     RGYHVMKGYYNNAEATREAIDEDGWLRTGDIAVMDEDGYIDITGRIKDVIIRGGENIY
                     PREVEEFLYKHPGISDVQIVGVPDPKYGEELMAWIIPKKGAAIDEEAVRSFCQGNISY
                     HKIPRYIKFIDEYPMTASGKIQKFKLREMSIAESLSCQI"
     gene            complement(2006..2371)
                     /locus_tag="VFX34_02520"
     CDS             complement(2006..2371)
                     /locus_tag="VFX34_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563810.1"
                     /translation="MPWTKNDYPDSFKNLEPDVRNKAIEIANALLREGNEEGRAISIA
                     TAKAREYVHGDSADRTKYEAKARNEDWILIKEGGERAIFKEDTKEDLLDKAKPYVNDH
                     NGILDVYHTDGSLESTLYE"
     gene            2624..4108
                     /gene="putP"
                     /locus_tag="VFX34_02525"
     CDS             2624..4108
                     /gene="putP"
                     /locus_tag="VFX34_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEX5563811.1"
                     /translation="MSSDFYQLIAIVVYMVAMLFIGWYAYRKTTNLNDYMLGGRSLGP
                     AVTALSAGAADMSGWLLMGLPGAIYAVGLTEAWIAIGLTVGAYLNWLLVAPRLRVYTQ
                     VSSDSITIPSYLESRLKDKSRLLRIASGIIILVFFTFYVSSGMVAGGKFFMSSFGLNY
                     HSGMLIVSAVVIAYTLFGGFLAVSYTDFVQGLTMFFALLLVPIIGLALTGGFAETAAS
                     IREVNPELLSLVKGATVAGVISSVAWGLGYFGQPHIIVRFMAIKSVKETKSARRIGIG
                     WMALSLFGAIATALVGIAYYQQNAGTELVDPETVFIALGQVIFHPFIAGIMLAAVLAA
                     IMSTISSQLIVTSSALIEDLYKAIIKSDATDKQYVFLGRMAVLVVSLIAMALAWPNNE
                     SILKLVSFAWAGFGGAFGPIILLSLYWRKLTATGALWGMVAGAITVGIWGTIDSLSDT
                     LYEIVPGFLICLVVAYFVSLMTYRPNSEIDKEFYDAMVLLKKER"
     gene            4190..>4499
                     /locus_tag="VFX34_02530"
     CDS             4190..>4499
                     /locus_tag="VFX34_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5563812.1"
                     /translation="MKLDHVVYFTSKDPSEIVEEQRNEGNHTVVGGRHEHWGTHNALM
                     YVKNAYVEWLSVERPEFAAQVDHPLPQLLLHDLKEKDGWGTICLSVTDIEQFNEDITN
                     K"
BASE COUNT         1157 a          935 c         1182 g         1225 t
ORIGIN      
        1 tatattttga cgatgcgtga aggcttgttt cggattgcag cagctgcggg actcgattca
       61 ccgacaaaat tcgagcggaa tcacgttgtt tacaaagacg aaagaggacg tgttttccct
      121 gttgaataaa gaacttgagt cgttttgcct aatcggcgaa acgacttttt ttgaagatga
      181 atacctaaga caccttctga attttgtgat aatatgtaaa taactataga atcatagaaa
      241 ggggttagat ggatgtcttt gttgagtaaa acggtgggcg aggttttaaa ggattgggcg
      301 aaggcgttgc caaagcatga ggcctatgtg tatccggagc atggtatcag aaggacctat
      361 gaagagtttg atcaagagac ggatgagctg gcgaaggcat tcatcgggat ggggatccag
      421 cgcggggagc atatcgccat ttggtccgat aacaaaccgc aatggttgct gagtcagttt
      481 gctacgggaa aaatgggggc tgtcctcgtt acggtgaata cgaattacca agcgaacgag
      541 ctcgaatatt tactgaaaca atcggacgcg acaacgctta ttctggatga agggttcaaa
      601 ggaacgtgct atatcgacat tatccgctcc atttgtccgg aattgatgga tagcaaaggc
      661 ggtgtcgttt ccagtgaaaa gcttcctgaa ttaaaaagaa tcatactcat gtcggatcga
      721 gaggaatccg ggatttataa atggtcggaa ttcctagccc atgctgatgg cattacggat
      781 gaggagttgg ctgagcggga gaagagtctg acgcctgacg atgtcatcaa tattcaatac
      841 acatccggga cgacaggttt tccgaaaggc gtcatgttga cccaccataa tattgtaaat
      901 aacgcaaaga tcatcgggga tttcatgaaa ctgacggaga aagaccgtct ttgcatcccc
      961 gtgccgttct tccattgctt cggcgcagtt ttgagcaatt tggcggcggt gacgcatggt
     1021 tcgacaatgg tcatcctgga gcagtttgac ccgctgcgtg tattggaggc ggtacaggaa
     1081 gagaaatgta cggcccttaa cggagtgccg acgatgttca ttgctgaatt gaaccatccc
     1141 gacttcaaaa aattcgacac atcgacattg cggaccggca tcatggcggg ctccacttgt
     1201 ccgatagagg tgatgaaaca ggtgatccat gacatgggtg cagatgaaat tacgatttgc
     1261 tacggacaga cggaagcatc acctgtcatt acacagacgc aaaccgatga tccgattgaa
     1321 aagcgagtgt ccactgtcgg aaaaccgcat ccttatgtcg aggtgaaaat catcgatccg
     1381 ttgacgggag acgagatgcc tgtcggcagt ccgggtgaac tgtgcacgcg cggctatcat
     1441 gtgatgaaag ggtactataa taatgcagaa gcgacgcgcg aggcgattga tgaggacggc
     1501 tggctccgca caggggacat cgccgtcatg gatgaagacg gctatataga cattacggga
     1561 cggataaaag acgtcatcat ccgcggtggg gaaaacatct acccacggga agtggaagag
     1621 ttcctttata aacatcctgg aatatcggat gttcaaatcg tgggggtgcc ggacccgaaa
     1681 tacggtgagg aattgatggc gtggatcatt cctaaaaagg gtgccgccat tgacgaggag
     1741 gctgtacggt cattttgcca aggcaatatt tcgtaccata aaattccgag gtacattaaa
     1801 ttcatcgacg aatatccgat gacagcatca gggaagatcc agaaattcaa acttcgtgaa
     1861 atgtcaattg ctgaatcttt gagttgccaa atatgaaacc caacaggcgg gggaactgta
     1921 aacgatccct cgcctgttta tttatttgca tcctatcatt ggtttgaaat tagcaatatg
     1981 gtccatccta aagggtgttg cgggatcatt catataacgt cgattcgagt gatccgtcag
     2041 tgtgatagac gtctagaatt ccattatgat cattgacgta tggcttggct ttatcaagta
     2101 gatcttcctt tgtgtcttcc ttgaaaatcg cccgctcgcc gccttctttt atgagaatcc
     2161 agtcctcatt gcgtgctttc gcctcatatt tcgtacggtc ggcactgtcg ccatgcacgt
     2221 attcccttgc ttttgcagtt gcgatggaaa tcgcacgtcc ttcttcgttt ccttcacgca
     2281 ataatgcatt cgctatttcg attgccttat tccttacgtc aggttcaagg tttttgaatg
     2341 aatcagggta atcgtttttt gtccacggca tgagggatcg cctccttacg taggtaattc
     2401 ccgctttctg cctgcttaaa cactaatccc atgtaattat tctgaatggg gtagaagtat
     2461 agtcaatgct gtcgaatcct gtgcatttcc cgggaaatgt gtaggaaatg atatgaattt
     2521 gtcccatatt ttccatcgca ggaataatga aattattttt cagaaattgc ttttaattga
     2581 taaaaagatg ttattataaa gaaaaatgaa taggagggaa attatgagca gtgattttta
     2641 tcaattgatt gccattgttg tgtacatggt ggccatgctc ttcatcggct ggtatgcata
     2701 caggaaaacg acaaatctga acgactacat gctgggagga cgttcactcg gacctgcagt
     2761 cactgcacta agtgccggcg cggcagacat gtcgggttgg ctgctcatgg gacttccggg
     2821 cgccatttat gcagttgggc tgacggaagc ttggattgcc atcggtttga cagtaggtgc
     2881 ctatttgaac tggctcctcg ttgcgccaag actccgcgtc tatacacaag tatcgagcga
     2941 ctcgattaca atcccaagtt atttggaaag ccggctgaag gataagtcgc gacttctacg
     3001 aattgcttcc ggaattatca tccttgtttt cttcacattc tatgtatctt caggcatggt
     3061 tgccggcggt aaattcttca tgagttcatt cggactgaat tatcattccg gaatgttgat
     3121 tgtttctgcg gtcgtcatcg cctatacgct ttttggagga tttctggccg taagttatac
     3181 cgatttcgtg caaggtctca caatgttctt cgctctttta ttggtaccga taatcggatt
     3241 ggcattgaca ggcggtttcg cggaaacggc ggcaagcatc cgagaagtga atcccgaatt
     3301 gctcagcctt gtcaaagggg cgacggtagc gggagtcatc tcttccgttg cttgggggct
     3361 cggttatttt gggcaaccgc atattatcgt ccgttttatg gcgatcaaat cggtgaagga
     3421 gacgaaaagc gctcgccgga tcggaatcgg ctggatggcg ctgagcttgt tcggtgctat
     3481 cgccactgcg ctggttggga ttgcgtatta ccagcaaaac gctggcacgg aattggtcga
     3541 tccggaaacg gtgttcattg cattagggca ggtcattttc catccattca ttgcgggaat
     3601 catgcttgca gcggtcctag ccgctattat gagtacgatt tcttcacagt tgatcgtcac
     3661 gtcatcggct cttattgaag atttgtacaa ggcgatcatc aaatcggatg cgacagataa
     3721 gcaatatgtc ttccttggcc ggatggcggt tctcgtcgtt tcattaatcg cgatggcact
     3781 ggcgtggccg aacaacgagt cgatcctgaa actcgtatca tttgcatggg ccggcttcgg
     3841 cggtgcattc ggaccgatca tcttactgtc cctttattgg cgcaagctta cagcgactgg
     3901 cgcattatgg ggaatggtgg caggtgcaat cacagtcgga atttggggaa cgatcgattc
     3961 actgtcggat actctttatg aaattgtgcc gggcttcctt atctgcctag tcgtcgcgta
     4021 ttttgtcagc ttgatgacgt accgtccgaa ctcggaaatc gacaaggaat tctatgacgc
     4081 aatggtttta ttgaaaaaag aaagataatg caaacgacct cccgcaaatg tcgcggaggt
     4141 cgtatttttt tggatatggt aatctattca gtagaggggg atgtatcaga tgaagcttga
     4201 tcatgttgtt tatttcacat cgaaagatcc tagtgaaatc gttgaagagc aaaggaatga
     4261 aggcaatcac actgtcgtgg gcggcaggca tgaacattgg gggacgcaca atgcgttgat
     4321 gtatgtgaaa aatgcatatg tggaatggct atccgtggag cgccccgaat ttgcggcgca
     4381 agtggaccat ccgttgcccc agctgctgct gcatgatttg aaagagaagg atggatgggg
     4441 cacaatatgt ttatcggtaa ccgatattga gcaatttaat gaggatatta cgaacaaag
//
LOCUS       DASULI010000116         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1117018,
            whole genome shotgun sequence.
ACCESSION   DASULI010000116 DASULI010000000
VERSION     DASULI010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117018"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /gene="walK"
                     /locus_tag="VFX34_02535"
     CDS             <1..1380
                     /gene="walK"
                     /locus_tag="VFX34_02535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEX5563813.1"
                     /translation="RVTPIINNNREVVGTLYVTANIEKVYKQINEINQILAGGVAVSL
                     TFTIIIGIFIARTFTRPISDMRRQAQAMAQGNFSRKVHVYGNDEMGQLAIAFNHLTNQ
                     LQESQSTTESEQRKLASVLENMTDGVISTDRKGRVSLINDSALMMLRMTRDLVLNRPI
                     ASILGLEQEYTFEDLIQVKDSIALDFSTEKEPYILRATFSVTQRETGFVNGLIVVLHD
                     NTEQEKIDMERREFVSNVSHELRTPLTTMRSYLDALAEGAWKNEEIAPSFLRVTQTET
                     ERMIRLVNDLLKLSRMDSKEYELNTEWVEFNHFFNSIIERFEFSKSQNVHFKRLLPSA
                     DLFVEIDTDKMTQVIDNIISNALKYSPDGGDVRFGVTTSGTYIKVMISDDGMGIPQSN
                     VNRIFDRFYRADRARSRAMGGTGLGLAIAKEMIIAHGGEIWAESEEGKGTTIFFTLPF
                     EQQEEGEWD"
     gene            1371..2714
                     /gene="yycH"
                     /locus_tag="VFX34_02540"
     CDS             1371..2714
                     /gene="yycH"
                     /locus_tag="VFX34_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HEX5563814.1"
                     /translation="MGLKYIEPIKSIILLLLVLLSVTFTFSIWTYSPQLDTIEQSPTV
                     DISIGASKQVEDIIKPYKFLIKYDESLNGTTEQEWINHILREMDTWKVSQLVREPNEM
                     NKGQMKAFFEQTNQFTLFFQGEVPLPLYDDILAIGDDQLSRIPEFSFDRLVVRWNPKE
                     IAFEAHFINRATGIHYHGKIAASDTMTSYRQIVQEGENLPAYVEVNAQDSAYLAVPAE
                     PVIMLRSTYFQEEISPTRFRDALFNDPNAVRRSQVDATHEEFGDDHALLGVNTETKML
                     NFAHPAAYSGEPAAYPSDLLMDAIDSINEHGGWTDSYRFSYISPYYRSVKFQLYIHGY
                     PVFSEPPLTGTTEIELFYGENRVFRYIRPYYTIDVSLNDEEEELPSGMTIVNELMEIE
                     NFDFGTVEEITTGYLMKHDPELRIYIMEPIWFYFAKGDWIPYTPELGGGEQIGLE"
     gene            2701..3537
                     /gene="yycI"
                     /locus_tag="VFX34_02545"
     CDS             2701..3537
                     /gene="yycI"
                     /locus_tag="VFX34_02545"
                     /inference="COORDINATES: protein motif:HMM:NF021186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEX5563815.1"
                     /translation="MDWNKTKTIFIVVFSILNIFLYSLYFNQRTEAQNVQVAGKTSIE
                     DLMKQENITYNVPQVVKNDFSYISANIKTFSEEELEPLEDQSIKIIDNTRIESEMDTP
                     VSILNSKEEYYFKDFLTKYVWNGAEYEFWEVDEEAQKAIFFQKVNGEPIFYSSNATLT
                     IYWDENYEVTHYEQRMLEEFSSEYHKKEVLTQDEAVGSLVTRGFLRPDSKVLKVTPGY
                     SSLVQLTGKQVFAPTWNIRVELKDGTIEHHFISAIEGKVIDFKLDKLEEEELDEAEDE
                     KE"
     gene            3550..>4213
                     /locus_tag="VFX34_02550"
     CDS             3550..>4213
                     /locus_tag="VFX34_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5563816.1"
                     /translation="MRFSVLASGSTGNSIYIETEEHAFLVDAGLSAKRMEQQLENIGR
                     SMTNVDGIFVTHEHSDHIKGIGVLARRYKVPIYANEKTWLAMNGLVGEIPTEQRFHFD
                     METNMSFGSLDIQSFAVSHDAADPMFYVFHENDRKLSIITDTGYVSDRMKGHIQGSDA
                     FVFESNHDVSMLQMGRYPWSVKRRILSDVGHVSNEDAAVAMSEVVAQKETRIYLSHLS
                     KDN"
BASE COUNT         1218 a          850 c         1086 g         1059 t
ORIGIN      
        1 cgtgtgacac cgattataaa caataatagg gaagtcgtcg gcacgctata tgtgacagcc
       61 aatattgaaa aagtgtacaa acagattaat gaaatcaatc aaatactcgc aggtggagtg
      121 gcggtttcct taacatttac aattattatc ggcatcttca tcgcccggac cttcacgaga
      181 ccgatttcgg atatgaggcg ccaggcacaa gcgatggcgc aaggcaactt ctccaggaag
      241 gttcacgtgt acgggaatga tgagatgggc cagctggcaa tcgctttcaa tcatttaacg
      301 aatcagctgc aagaatcaca gtcgacgacg gaaagtgagc aaaggaagct cgcctcggtt
      361 cttgaaaata tgacggacgg cgtcatttca acggatcgta aaggcagggt gagtctgatc
      421 aatgactctg cgctcatgat gctgaggatg acacgcgacc tcgtgctgaa tcggccgatc
      481 gcaagcatcc taggtttgga gcaggagtat acgttcgagg acctcattca agtgaaggat
      541 tccattgcgc tcgacttcag tacggaaaaa gagccttaca tcctgcgggc cactttttct
      601 gtgacgcagc gggagacggg ctttgtgaat ggtcttatcg tcgtcctgca tgacaatacc
      661 gagcaggaga agatcgatat ggagaggcgg gaattcgtct ccaacgtttc ccatgagctt
      721 cgcacgccgc tgacgacgat gcgcagctat ttggatgcat tggcggaagg tgcttggaag
      781 aacgaggaga tcgcgccttc cttcctccga gtgacacaga cggagacaga gcggatgatc
      841 cggctcgtca atgacctgtt gaaactttcc aggatggaca gtaaagaata tgaattgaac
      901 accgaatggg ttgagttcaa tcatttcttc aactcgatca ttgaacggtt tgaattctcg
      961 aagtcgcaga acgtgcattt caaacgtctg ctgccttctg cagatctttt tgttgaaatc
     1021 gatacggata agatgacaca ggtcatcgat aatatcattt cgaatgcgct taaatattct
     1081 ccggatggcg gggatgtacg ctttggagtg acgacatcag ggacttacat caaagtgatg
     1141 atttccgacg atggaatggg tatcccgcag tcgaatgtga accgtatttt cgataggttc
     1201 tatagagccg atcgcgcaag atcgcgagcg atgggcggga caggccttgg ccttgccatc
     1261 gcaaaggaaa tgatcattgc acatggcggg gaaatatggg cggaaagtga agaaggcaag
     1321 gggacaacaa tattctttac attgcctttt gaacaacaag aggaaggtga gtgggattga
     1381 agtatatcga gccgattaaa tcgatcatct tactattgct cgtcttattg agcgtcacat
     1441 tcaccttttc catatggacg tattctccgc agttggatac gattgaacaa tcgccgaccg
     1501 ttgacatttc catcggtgcg tcgaagcaag tagaggatat tatcaagccc tataagtttc
     1561 taattaaata tgatgaatcg cttaacggaa caaccgagca ggagtggatc aatcatattt
     1621 tgagggaaat ggacacgtgg aaagtctctc aactggtgcg ggaaccaaac gagatgaaca
     1681 agggacagat gaaggctttt ttcgaacaga cgaatcagtt cacattgttt ttccaaggtg
     1741 aagtgccact gccgttatac gatgatattt tggcaattgg ggatgatcaa ctttcccgaa
     1801 ttccggaatt cagctttgac cgacttgtcg tgagatggaa tccgaaagaa atcgcctttg
     1861 aagctcattt catcaaccgt gcaacgggaa ttcattacca tggaaaaata gcagcctcag
     1921 atacgatgac gtcgtatcgt caaatcgtcc aggaagggga aaacctgcct gcctacgtgg
     1981 aagtgaatgc tcaggattct gcttatttgg cggtgccggc tgaaccggtc ataatgctac
     2041 gaagcacata ttttcaggaa gaaatcagtc cgacccgctt ccgggatgct ctattcaatg
     2101 atccgaatgc agtgcgtcgc agccaagtgg atgcgacgca tgaagaattc ggtgacgatc
     2161 atgcgctgct aggcgtgaat acggaaacga agatgctgaa ttttgcccat cccgctgcgt
     2221 atagcgggga accggcagca tacccttccg acttgttgat ggatgccatt gattccatta
     2281 atgagcatgg cggctggaca gattcgtaca ggttctcgta tataagtcct tattacagat
     2341 ctgtgaaatt ccaactgtac attcatggat atccggtttt cagcgagccc ccgcttacgg
     2401 gcacgacgga aattgaacta ttttatggag aaaatcgggt tttccgatat atccggccgt
     2461 actacacgat agatgtgtcg ctgaatgatg aagaggaaga gcttccttca gggatgacaa
     2521 ttgtaaacga actaatggaa attgagaact tcgattttgg gacggtcgag gaaatcacca
     2581 ccggctattt gatgaaacat gacccggaat taaggattta tataatggag cctatctggt
     2641 tttattttgc caaaggagat tggattccat atacacctga actaggcggg ggtgagcaga
     2701 ttggattgga ataaaacgaa aacaatcttc atcgtcgttt tttcgatatt gaatattttc
     2761 ctttattcct tgtattttaa tcagcgaacc gaggcgcaaa atgtgcaggt cgctggaaaa
     2821 acgtcaattg aggatttgat gaaacaggag aatattacgt acaatgtacc gcaagtggtg
     2881 aaaaatgact tttcctatat ctccgcaaat ataaagacct tctcggagga ggaattggag
     2941 ccgcttgagg atcagtccat taaaatcatc gacaatacaa ggattgagtc cgaaatggac
     3001 actcctgtat ctatcctgaa ttcaaaagaa gaatattatt tcaaggattt tctgacgaag
     3061 tatgtttgga acggagccga atatgaattt tgggaagtgg atgaggaagc acagaaagcc
     3121 atcttctttc aaaaagtaaa tggggaaccg atattttaca gctcgaatgc gacgttgaca
     3181 atctattggg acgagaacta tgaagtgacc cattacgaac agcggatgct ggaggaattt
     3241 tcaagcgaat accataaaaa agaagtgttg acgcaagatg aagcggtcgg atcactcgta
     3301 acgcgggggt ttttgaggcc ggattccaaa gtgttgaaag tgacgccagg atactcgtcg
     3361 ctcgtccaat tgaccggaaa gcaagtattt gccccgacgt ggaatattcg tgtagaatta
     3421 aaggatggca ccatcgaaca tcacttcatc agtgccattg aaggtaaagt gatagacttc
     3481 aaattggaca agttggaaga ggaagaactt gatgaggctg aggatgaaaa agaataagga
     3541 gtttttcata tgcgtttcag tgtactcgca agcggaagta cgggcaattc catatatata
     3601 gagaccgaag agcatgcttt tctggtcgat gctggactaa gtgcaaagag gatggagcag
     3661 cagcttgaaa atatcggccg ctccatgacg aatgtagacg gcatttttgt cacccatgag
     3721 catagcgatc atattaaagg gattggtgtg ctcgctagaa gatataaagt acccatttat
     3781 gcgaatgaaa agacatggct tgcgatgaat ggacttgtcg gtgagatacc gacagagcag
     3841 cgtttccatt tcgatatgga gacgaatatg tcatttggtt cgctcgatat ccagtcattc
     3901 gctgtttccc atgacgcagc ggatccgatg ttctacgttt tccatgaaaa cgatcgaaaa
     3961 ctatcgatca tcacggatac ggggtatgtg agcgaccgga tgaaagggca tatccaaggg
     4021 tcggatgcat tcgttttcga aagcaatcat gacgtcagca tgcttcaaat gggacgttat
     4081 ccttggtctg tcaaacgccg gatcctgagc gacgtcgggc acgtgtcgaa tgaagatgcc
     4141 gccgttgcga tgagtgaagt cgttgcccaa aaagagacga gaatttattt atcgcatctg
     4201 agcaaggata ata
//
LOCUS       DASULI010000117         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1609492,
            whole genome shotgun sequence.
ACCESSION   DASULI010000117 DASULI010000000
VERSION     DASULI010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609492"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1026
                     /locus_tag="VFX34_02555"
     CDS             46..1026
                     /locus_tag="VFX34_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563817.1"
                     /translation="MMKRLTACILFIFLLSGCNPKPQEEVEEVLEDDIVTIKNVEKLV
                     EVETFFKTRDFQLEGLERMYRYYGNDFPAEEQERIAIYEDFERISYVDFMDLMAEHGI
                     DTLPSYKKAEDIDRILKEGKPVYANFSLINGERWDVLFYGYSEEDFAYYNLVSGVKKT
                     IEKKRIEALGEFDTVIPYKVGELTAAEKEKSYLYVRQRLTDAYWKDDPIKMRKYLDIA
                     EQNKWLDGLNYGYYFIYHYLFHDPQPERAAKYVEAELNRWREPATLEAALKYYILTND
                     ELEIKRMIRDIQIMPWHRRETLQFIIDNGADYGYPEKATEATEILKKKKQ"
     gene            1435..2016
                     /gene="fapR"
                     /locus_tag="VFX34_02560"
     CDS             1435..2016
                     /gene="fapR"
                     /locus_tag="VFX34_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HEX5563818.1"
                     /translation="MRVPKRERQRQLEMSLEQNPFLTDEELSKQFKVSVQTIRLDRLE
                     CGIPELRERLKTVAARTIDEEVKSLQSDEVIGEIIDIELDKRAVSIFDVTEDHVFRRS
                     GIARGHHLFAQANSLAVAVLDDDLALTVTSNLHFVKPVKSGDRVIARAIVDKERSNDK
                     RTFVDVVSTVENVTVFTGEFQMYQTTDHSRREI"
     gene            2016..>2991
                     /gene="plsX"
                     /locus_tag="VFX34_02565"
     CDS             2016..>2991
                     /gene="plsX"
                     /locus_tag="VFX34_02565"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX5563819.1"
                     /translation="MIIALDGMGGDHAPGEIIEGALLSLSEFEDIHIHIYGNEEKMAP
                     YLKDHARLKVIHCTERIEADDEPVRAIRRKKDASMVRMAQSVKDGKADACVSAGNTGA
                     LMAAGLFIVGRIEGIDRPALSPTLPTIDGQGFVFLDVGANSDAKPGHLQQYAIMGSIY
                     AEKVRGIQKPRVGLLNIGTEEGKGNELTKAAYDLLSEAPIHFVGNVESRDILNGAADV
                     IVTDGFTGNMVLKTIEGTAAGFFSMLKEVYGASLKSKLSAAMVKDDLRGLKKKMDYTE
                     YGGAGLFGLNSPVIKAHGSSNANAILNAIRQARTMVEFDVCGTIRKTVG"
BASE COUNT          915 a          545 c          773 g          758 t
ORIGIN      
        1 cggaacggtg aataagattg agtcggatag gttagaggtt ctcagatgat gaaacgatta
       61 actgcctgca tactatttat atttctcctt agcgggtgca atccaaaacc acaggaagaa
      121 gttgaagagg tacttgagga cgatatcgtg acaattaaaa atgtggaaaa actggtagaa
      181 gttgaaacat tctttaagac tagggatttt cagttagaag gattagagcg tatgtatcga
      241 tactatggaa atgactttcc cgctgaagag caggagagaa tagcgatata cgaagatttt
      301 gaaagaatct cctacgttga tttcatggat cttatggctg aacatggcat agatacattg
      361 ccctcttaca aaaaagctga agatattgat cggatcctta aagaagggaa gccggtatat
      421 gcaaattttt cgttgattaa tggagaaagg tgggatgtcc tcttttatgg ctattcggag
      481 gaggacttcg catattacaa tttagtttca ggcgtgaaga aaacaatcga gaaaaaacgc
      541 attgaggcgc ttggagaatt cgacacggtt attccgtata aagtgggaga acttacagcg
      601 gccgaaaaag agaaatccta tctgtacgtt aggcaaaggc taacagatgc ttattggaaa
      661 gacgatccaa tcaaaatgag aaagtattta gatattgctg aacaaaacaa gtggttggat
      721 ggacttaact acggttatta ctttatctac cattatctat ttcatgaccc tcagccggag
      781 cgtgccgcta aatatgttga agcggaactg aataggtggc gtgaaccggc cacacttgaa
      841 gccgctttga aatattacat tttgacgaat gacgaattgg aaattaaacg gatgatacgc
      901 gatattcaaa tcatgccgtg gcatcgcagg gaaacgcttc aattcattat cgacaatggc
      961 gccgactatg gctatccgga aaaagcgaca gaagcaacag agatattaaa gaaaaagaag
     1021 caatgaaaaa acacagactt tcccttgggt gggaagggag gctgtgtttt tctgtgctta
     1081 taaactgtac tctttgaccg gaggcgggaa ctaatcgcgg cgaaacgtat ataaaagcga
     1141 taattcaatc tgttctcgat gtgaaagcgt taaggaattt cgttacacgc gattcaatat
     1201 atcaaatgcg ggaaatggcg ctaatctttt ataccgatcg gcgccaatag tatatttaag
     1261 gtccatttat tatgtgattt ggttactgat ataaatgcgc cctccggaaa gcgtgccgtt
     1321 tggagcgtaa atcaaccggt taaagattac atgcaatgtt ttgtagaaat cgatgaggaa
     1381 ctatgataat atactggtat tagtaccaag tgctaatccg gaagggtgaa tcgagtgaga
     1441 gtgccaaaaa gggagaggca gcgccagctt gaaatgagcc tcgaacaaaa tccttttctg
     1501 acagacgaag aattatccaa acaatttaaa gtaagcgttc aaacaatccg gttggatcga
     1561 ttggaatgtg gaatcccgga attgcgtgaa cgattgaaaa cggtagctgc acgaacgatc
     1621 gatgaagagg tgaaatccct ccaatccgat gaagtgattg gtgaaatcat cgatatcgag
     1681 ctggacaaga gggctgtttc catattcgac gtaacggaag atcatgtctt ccgaaggagc
     1741 ggaatcgctc gtggccatca tctattcgct caggcaaatt ctttggcagt ggccgttctc
     1801 gacgacgatc ttgcattgac tgtcacatcg aaccttcatt tcgtgaagcc tgtcaaatcg
     1861 ggtgaccggg tcattgcacg tgccatcgtt gataaagagc gttcaaatga taaaaggact
     1921 tttgtcgatg ttgtctctac cgtcgaaaac gtgacggttt tcaccggtga attccagatg
     1981 tatcaaacta cggaccatag taggagggaa atatgatgat cattgcatta gatgggatgg
     2041 gcggagatca tgcaccgggt gaaattattg aaggcgcact tctgagtctc tcggaatttg
     2101 aggatataca tatacatatt tatggaaatg aagaaaagat ggctccttat ttgaaagacc
     2161 atgcacgctt gaaagtgatt cattgcacag aaagaatcga agcagacgat gagccggtcc
     2221 gcgcgataag acgtaagaag gatgcctcca tggtcaggat ggcacaatct gtaaaagacg
     2281 gaaaagccga tgcctgtgtt tccgcgggga acacgggtgc acttatggcg gcaggccttt
     2341 tcattgtcgg gcggattgaa ggcatcgatc gtcctgcatt atcaccgacc ttgccgacga
     2401 tcgatggaca aggtttcgtc ttcttggatg tcggtgcgaa ttcagatgca aagccgggcc
     2461 atttgcagca atatgcaatc atgggcagca tctatgcgga aaaggtccgt ggcattcaaa
     2521 agccccgcgt tggactgttg aatatcggaa ctgaagaagg caaagggaat gaattgacga
     2581 aagcggcata cgatcttctt tcagaagcgc caattcattt tgtcggtaat gttgaatcaa
     2641 gggatatttt aaacggtgcg gcagatgtca ttgtgacgga cggatttaca gggaatatgg
     2701 tgctgaagac gattgaaggg acagctgcag gattcttttc aatgctgaaa gaagtatatg
     2761 gagcctcatt gaagtccaag ctgtctgcgg caatggtgaa agacgacctc cgtggcttga
     2821 agaagaaaat ggattacacc gaatatggcg gggcgggatt gtttgggctg aattcgcctg
     2881 tcatcaaggc gcatggctcc tcgaatgcca atgcgatttt aaatgcaatc agacaagcca
     2941 ggacaatggt cgaatttgat gtgtgcggga cgatccgaaa gacagttgga a
//
LOCUS       DASULI010000118         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1071999,
            whole genome shotgun sequence.
ACCESSION   DASULI010000118 DASULI010000000
VERSION     DASULI010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071999"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VFX34_02570"
     CDS             <1..1354
                     /locus_tag="VFX34_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX5563820.1"
                     /translation="GELPFSAETAVAIALKHLQEETPSVRIAFPGIPQSVENVILKAT
                     AKDATYRYQSADEMYDDLLTVLSAERQHEEKFAIPYDDDRTRVIPVIADTSKADHSEK
                     TKIIDPVIPEPAPIQPKKKRKKWPLIVGLTALLLAVAALLIIFWPTLFGPKTALIPDV
                     VGLEEANAVSLLEENGFVVEDRVEDYSDEFPAGQVFGTNPEEGKKREIGSPITIYVSI
                     GKQTTELTDYVGRDYEEIKNFLEGFKFRNIDPEYVYSDEPKGKILKQTPEAGEKVVPE
                     ETDLVFTVSAGKEMKTLEDLTKYNEGELKDYAKRSGFNIRIVDRVESDTVKEGYVISQ
                     DPSANSELEKGARVDVVLSKGKAKKPVKYFVDTVYIPYEPSNNDDEENSNPEPQTIKI
                     YIQDSKHSMTDPVAEFTITEDVQREITIQLEEGMRGGYRVHRDSSIIAEKTFDYDDVE
                     "
     gene            1369..2253
                     /gene="rsgA"
                     /locus_tag="VFX34_02575"
     CDS             1369..2253
                     /gene="rsgA"
                     /locus_tag="VFX34_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017151228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX5563821.1"
                     /translation="MPEGQIRKAISGFYYVKSEDGNLVQCKGRGVFKIRGISPLVGDF
                     VTIVQDGDNDATITEVHERKNELVRPPIANVDQALLVFSIVEPDFSLHLLDRFLTVIE
                     SVGIKPVICLTKKDVAGEKDLQQAESATDYYRGIGYDVIGTFIGDPELSPLLQPYLEG
                     KTTVLAGQSGVGKSTLLNTLIPELSLKTGEISEALGRGRHTTRHVELLEVAGGLVADT
                     PGFSSLDFDHIEKEELRDYFIEMESVAHACKFRGCLHLKEPGCAVKEKVETGEILRSR
                     YTNYLQFLQEITDRKPRY"
BASE COUNT          699 a          455 c          599 g          561 t
ORIGIN      
        1 gggcgaactt cctttctccg cagagactgc ggttgcgatt gcattaaaac atttacagga
       61 agagactcct tctgtccgaa ttgcgtttcc tggcattccg caaagtgttg aaaatgtcat
      121 tttgaaagcg acagcgaaag atgctacata tagataccaa tcggctgacg agatgtatga
      181 tgatttgctg acggttttat cagccgaaag gcagcatgaa gagaaattcg cgattcccta
      241 tgatgatgac cgtactcgag tcatccctgt tatagctgat acttcaaagg cggatcactc
      301 ggaaaagacg aaaatcatcg atccagtcat tcccgaacct gctccaattc agccaaagaa
      361 gaagcggaaa aaatggccgc tcatcgtcgg ccttactgca ttgctgttgg cggttgccgc
      421 tctcctgatc attttttggc caaccttatt cggtccaaaa actgccttga ttccggatgt
      481 ggtcggtttg gaggaagcaa atgctgtttc attattggag gaaaatggct ttgttgttga
      541 agatagagtg gaggattatt cggatgaatt ccccgctgga caagtttttg gaacaaatcc
      601 cgaggaaggg aaaaaacgtg agatcggatc gccgatcact atatacgtta gcatcggcaa
      661 gcaaacgacg gaactgaccg attatgtcgg tagagattat gaggaaataa agaatttctt
      721 ggaaggattc aaattcagga atatcgaccc tgaatatgta tacagcgatg aaccgaaagg
      781 gaagatcttg aagcagacgc ctgaggcagg agaaaaggta gttccggaag aaacagatct
      841 tgtctttaca gtcagtgcgg ggaaagaaat gaagacgctt gaggacttga ccaaatacaa
      901 cgagggagaa ttaaaggatt atgcgaaaag atccggcttc aatatccgga tcgtggacag
      961 agtagaatct gatacggtga aggaaggcta tgtcatttca caagatcctt ctgcgaactc
     1021 tgaactggaa aaaggtgcga gggtggatgt cgtcctttcg aaaggcaagg cgaaaaaacc
     1081 agtaaagtat ttcgtggata cggtttacat tccatatgaa ccatcgaaca atgatgatga
     1141 agaaaactcg aacccggaac cgcaaacaat caaaatctat atccaggaca gcaagcattc
     1201 gatgacggat ccggttgccg aatttactat aacggaagat gtacaaagag agataacaat
     1261 ccaattggaa gaaggaatgc gcggcggtta cagagtccac cgtgactcat cgatcattgc
     1321 agaaaaaaca ttcgattatg atgatgtcga ataagaaaga gggattggat gccggaagga
     1381 caaatcagaa aagcgatcag cggtttttat tatgtcaaaa gtgaagatgg gaatctcgtc
     1441 cagtgcaaag gtcgaggtgt tttcaaaatc aggggcatat caccccttgt cggcgatttt
     1501 gtcacgattg tccaagacgg tgataatgat gcgacaatca cggaagtgca cgaaaggaaa
     1561 aacgagctcg tgcgaccgcc catcgccaat gtggatcagg ctctgcttgt gttttcgatt
     1621 gtagagcctg atttctccct tcaccttttg gacagattcc ttactgtaat tgagtcggtc
     1681 ggcatcaagc cggtcatctg tctgacaaaa aaagatgtgg cgggggaaaa ggatcttcaa
     1741 caagcagaat ccgcaacaga ctactaccgg ggaatcggct acgatgtgat cggaactttt
     1801 atcggagatc cggaactttc ccctttgtta cagccttatt tggaaggaaa gacgacagtt
     1861 cttgcgggtc agtccggtgt cgggaagtcg actttgctga atacgttaat acccgagcta
     1921 tcattgaaga ccggcgagat ttccgaagca cttggcagag gaaggcatac aacccgtcat
     1981 gtggagcttt tggaagtggc gggtggcctt gtcgctgata cgcccggttt tagttcactt
     2041 gatttcgatc atatcgaaaa agaggaatta agggactatt tcattgaaat ggaaagcgtg
     2101 gcgcatgcgt gcaaattcag agggtgtctg catctgaaag agccaggatg tgcagtgaaa
     2161 gaaaaagtgg aaacggggga aatactgcga tcccgttaca caaattattt acagtttcta
     2221 caagagatta ctgaccgaaa gccgaggtat tgacaatgat taaaatagct ccgtccattt
     2281 tggcggcgga tttttcaaag cttgcagatg aagt
//
LOCUS       DASULI010000119         4470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1525126,
            whole genome shotgun sequence.
ACCESSION   DASULI010000119 DASULI010000000
VERSION     DASULI010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525126"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFX34_02580"
     CDS             complement(<1..474)
                     /locus_tag="VFX34_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX5563822.1"
                     /translation="MAITSGGLVLLKEMRHVLPPSEQKIADYILENPQDIVTMTVSEL
                     GEVSQTSGAAVTRLCKSLQLKGFQELKLRINGDLGRKLEITTRDIQPNEPVATTIQKV
                     TEQAVHTLLETAELLDPKQLEHAVDAIVQAKNIHFFGVGASGIAAIDAQQKFLRIH"
     gene            complement(494..1441)
                     /locus_tag="VFX34_02585"
     CDS             complement(494..1441)
                     /locus_tag="VFX34_02585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007132711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5563823.1"
                     /translation="MYRTFQSGDERGIVELWNDVLIHDPVNEKRFRTQVLLDANFDPE
                     GLIVAVDGERIVGAILAITRKLPMMGTDLEPDTGWITFFMVHPDVGRQGIGRGLMEAA
                     SDYIRNQGAKKVFFSSYAPNYFLPGIDETAYPSGFNFLQKEGFRRAYSPVAMHRALTD
                     YVYPEAVKAVKAKREQEGYVFESVQDGDLPAVIRFANTVFNPDWGRAIREGLMQGLDP
                     SQVLIAKKDGKVVGFAMYGGYEGIRERFGPFGVDDSEQGKGLGKILLHESLHSMKQRT
                     IQGAWFLWTSEKSSAGHLYVKNGFKTYRTFHVMMKELTE"
     gene            complement(1447..2370)
                     /gene="murQ"
                     /locus_tag="VFX34_02590"
     CDS             complement(1447..2370)
                     /gene="murQ"
                     /locus_tag="VFX34_02590"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX5563824.1"
                     /translation="MEKLSLLMTEGSNPKTSRLDEMSIGDILKTMNDEDQTVALAVRQ
                     VIPQIEKAVEKVAEAFKSGGRLLYVGAGTSGRVGVMDAVECPPTFGTSPDLVKAVLAG
                     GKDAMYEAVEGAEDDEALGAKDLEDLQITGHDVVIGIAASGRTPYVKGALEYANSCGA
                     TTVSLSNNENSLISNYADIPIEVVTGPEILTGSTRLRAATSHKLILNMISTTAMVKTG
                     KVYQNLMVDVNASNFKLRERAKKMVCTITELDEDKAEAVLEETGYNVKQAIVMILAEV
                     DKEKAQVLIETANGFVGKAVKLASLEEREER"
     gene            complement(2382..3554)
                     /locus_tag="VFX34_02595"
     CDS             complement(2382..3554)
                     /locus_tag="VFX34_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX5563825.1"
                     /translation="MMMERNLVKLGVDVFFERDLSELRGKRLGLLTNQTGINAELTST
                     IRLFHEHPELQLTALFAPEHGLLTNAKEGEKFTDSVHRSTGIPVFSLYGQTKKPSDAM
                     MEQVDVAVFDIQDIGARYYTYIYTMAYMMEACARTGKRMIVLDRPNPIGGEGVEGNLI
                     DPDFTSFVGLYPIPNRHGMTVGELAVYFNEEFRIGCDLEVVGMEGWKRSSFHTENGLP
                     WIPPSPNTTSLDMMLLYPGTCLLEGTNLSEGRGTTQPFEIMGAPFIDGEMLQDHVNAL
                     GLEGGRARALAFTPTYQKYAGELCEGIQLHLTDRRAFKPVESVARILAVIASIYADKL
                     EFLEYGNLRHPMFDLLAGNNALRHGLLKGDIHPYLQQCGRDAEKFMETRERYLRYR"
     gene            complement(3551..>4470)
                     /locus_tag="VFX34_02600"
     CDS             complement(3551..>4470)
                     /locus_tag="VFX34_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX5563826.1"
                     /translation="TVFAVVSLLPLYWVFSTSLQLSSYQSQDMERPVSYVDSNPPKMY
                     PMGIPLYFKHWGEAREAAKAGDVQEEAYHRDMMDHIVSNSFGAFKTLFKKHDVGKWFF
                     NSIYIAVVVTIGILLIDSMAGYVLAKKKFPGRNLIFWIIISTMMIPGQVTLVPLFIMV
                     GNLGLMDTHWALILPDLSMVFGVFLMRQFMYSIPDELLEAARMDGASEWRTFWTVVLP
                     LARPGLAALGIFTFMNVWNSFLWPIIVLNTAELYTLPVGLKTLQDANLASFKLLMSGA
                     AVAAIPMIIVFILFQRYFVQGLTLGGVKE"
BASE COUNT         1062 a         1314 c          957 g         1137 t
ORIGIN      
        1 atggatccgc aaaaacttct gttgggcgtc gattgcggct atccctgaag cccctacacc
       61 gaaaaagtgg atattcttcg cttggacaat cgcgtcgaca gcatgttcca attgtttcgg
      121 atcgagcaat tccgcagttt cgagcaatgt atggaccgct tgctccgtca ctttctggat
      181 cgtcgttgcc accggttcat tcggctggat gtcccgtgtc gtaatttcca gtttccgacc
      241 gagatcgcca ttgatgcgta atttcaattc ttggaagcct ttcaattgca aagatttaca
      301 tagcctcgta acagctgcac cactcgtttg gctcacttct ccaagttccg aaacggtcat
      361 cgtcacgatg tcctgcgggt tctccaaaat gtaatcggca attttctgtt ccgaaggagg
      421 gagcacatgc ctcatttctt tcaacagcac taagccgccg ctcgttatcg ccatctccgt
      481 ccctcctctt tcttcattct gtcaattcct tcatcattac atgaaatgtc cggtatgtct
      541 taaatccgtt tttcacatat aaatgccccg ccgagctttt ttcactcgtc cagaggaacc
      601 aagccccttg gatcgtcctt tgcttcattg aatgaaggga ttcatgcaat aagatcttcc
      661 cgagcccttt gccttgctcg ctgtcgtcca ctccgaaagg tccgaaccgt tcccggatcc
      721 cttcgtagcc gccatacatt gcgaagccga cgactttccc atcctttttt gcaatcaaca
      781 cttgggaagg atcgagtcct tgcattaagc cttcgcggat tgccctgccc caatccggat
      841 tgaatactgt atttgcgaac cggatgacag caggcaaatc gccgtcctgg acggattcaa
      901 atacatagcc ttcctgctcc cgcttcgcct tcaccgcctt cactgcttcg ggatagacat
      961 aatccgttaa cgcccggtgc atggcgacag gtgaataggc acgcctgaat ccctcctttt
     1021 gaaggaaatt gaaaccggag ggataggctg tttcatcaat cccgggaagg aaataattcg
     1081 gcgcataaga agagaagaac accttcttcg ccccttgatt gcggatgtaa tcacttgccg
     1141 cttccatcag cccccggcca atcccttgcc tccccacatc gggatgcacc atgaaaaagg
     1201 tgatccagcc cgtatcaggt tcaagatcgg tccccatcat cggcagtttc cgggtgatgg
     1261 ccaagatcgc cccgacaatc cgctctccgt caacggcgac aatcaagcct tccgggtcga
     1321 agttcgcatc gagcagcact tgtgtcctga agcgcttttc attgaccgga tcgtggatca
     1381 atacatcatt ccacaattcc acaatccctc tctcatcacc gctttgaaat gtccgataca
     1441 tggagctcac ctctcttccc tttcttccaa tgaagcaagt ttaacggctt tgcctacgaa
     1501 cccatttgcc gtttcaatca aaacttgggc cttttcctta tcgacttccg caaggatcat
     1561 gacaatcgct tgcttcacat tgtagccggt ctcttcgagt acggcttccg ccttgtcttc
     1621 atccagttcc gtaatcgtgc aaaccatctt tttcgcacgc tctcgcaatt tgaaattgct
     1681 tgcattcaca tcgaccatca agttttgata caccttgccg gtcttcacca tcgccgtcgt
     1741 ggaaatcata tttaaaatca atttatgtga tgtcgctgca cgcagccttg ttgaccccgt
     1801 cagtatttcc ggaccggtaa cgacttcgat cggtatgtcc gcatagttgc tgatgagcga
     1861 gttttcatta ttggaaaggc tgacggtcgt tgcgccgcat gaattcgcgt actcgagcgc
     1921 ccccttcaca taaggtgtcc ggccgcttgc ggcgatgccg atgacgacat catgccctgt
     1981 gatttgaagg tcctccaaat ccttcgcccc aagcgcctca tcatcctcgg caccttcgac
     2041 tgcctcatac atcgcatctt tgccgcctgc caacactgcc tttacgaggt cgggggaagt
     2101 gccgaacgtc ggaggacatt ccactgcgtc catgacaccg accctgccgc tcgttcctgc
     2161 cccgacataa aggagcctgc cgccactctt aaaagcctcc gccacttttt caaccgcctt
     2221 ttcaatttgc gggatcactt gccggacagc taaagcaacg gtctgatcct cgtcattcat
     2281 cgttttcagt atgtccccga tcgacatttc atccaaccgc gacgtctttg gattggatcc
     2341 ttcagtcatc agaagtgata atttctccat cgtccgcctt cctatctata tcgtaagtaa
     2401 cgttctctcg tttccatgaa tttttccgcg tcccggccgc attgctgtaa atacggatga
     2461 atatcccctt tcagcaaacc atgccgcaat gcattattcc ctgccagcag gtcgaacatc
     2521 ggatgccgca aattgccata ttccagaaac tcgagcttat cggcatatat cgatgcaatg
     2581 actgcaagaa tccgggcgac cgactccacc ggcttgaacg cccgccgatc cgtaagatgc
     2641 agctggatcc cttcgcaaag ctctccagca tacttttgat acgtcggcgt gaaggcgagc
     2701 gcacgcgccc ggcccccttc caaaccgagg gcatttacat gatcctgcag catctcgccg
     2761 tcaatgaaag gggctcccat aatttcaaaa ggctgcgtcg tcccgcgtcc ttccgataga
     2821 ttcgtccctt ccaacaaaca tgttcccggg tacaacaaca tcatatcgag cgatgtcgta
     2881 ttcggcgaag gcggaatcca aggcagccca ttctccgtgt ggaatgaact tcgtttccag
     2941 ccttccatcc cgacgacttc caaatcgcat ccgatgcgaa actcttcatt gaaatagaca
     3001 gccaattcac cgactgtcat cccgtgccga ttcggaatcg gatagagccc gacgaaggaa
     3061 gtaaaatccg gatcgattaa attcccttcg accccctccc cgccgatcgg attcggccga
     3121 tcgaggacga tcatcctttt gcccgtccgc gcacaagctt ccatcatata cgccatcgtg
     3181 tatatatacg tgtaatacct tgcgccgata tcctgtatat cgaaaacggc aacatcgacc
     3241 tgctccatca ttgcatctga cggtttcttc gtctgcccat aaaggctgaa tacaggaatg
     3301 ccagttgatc gatggacgga atcggtgaat ttctccccct ccttcgcatt tgtcagcagt
     3361 ccgtgttccg gggcaaaaag ggccgtcagc tgcagctccg gatgttcatg gaaaagccgg
     3421 attgtcgaag tcaattcggc atttatgcct gtttgattcg tcaataaacc gagccttttc
     3481 ccccgcagtt ccgacagatc cctttcgaag aatacgtcaa caccgagttt taccaggttt
     3541 cgctccatca tcattcctta acgccgccaa gcgtcaagcc ttggacaaaa tagcgctgga
     3601 acaggatgaa tacgatgatc atcggaatcg ctgcaaccgc agcaccgctc atgagcaatt
     3661 taaagcttgc caagtttgcg tcctgcaatg ttttcaagcc aaccggcaat gtatacagtt
     3721 ctgcggtgtt cagcacgatg atcggccaaa ggaatgaatt ccagacattc atgaacgtga
     3781 agatgccgag cgcggcaaga cctggtcttg caagcggcaa aacaaccgtc caaaatgtcc
     3841 gccattcact tgcaccgtcc attcgtgcag cctccagcaa ttcatccgga atggaataca
     3901 tgaactgcct catgaggaag accccgaata ccatcgaaag atcgggcagg atcaacgccc
     3961 aatgcgtgtc catgagacca aggttcccga ccattataaa taatggtaca agcgtcactt
     4021 gaccgggaat catcatcgtt gaaatgatga tccagaagat caggttccgc cccgggaact
     4081 tcttctttgc cagaacatat cccgccatcg agtcgatgag caagatacct atcgtgacga
     4141 cgaccgcaat ataaatgctg ttgaaaaacc atttcccgac atcatgcttc ttgaacaacg
     4201 tcttgaatgc tccgaacgaa ttcgagacga tatgatccat catgtcgcga tggtacgctt
     4261 cttcttgtac atcaccagct ttagccgctt ccctcgcctc gccccaatgt ttgaagtaaa
     4321 gcggaatccc catcggatac attttcggcg gatttgaatc gacatacgaa accggccgct
     4381 ccatatcttg ggattggtag ctgcttaatt gaagggacgt cgagaacacc caatatagcg
     4441 gaagcaaaga aacaacagcg aaaacagtta
//
LOCUS       DASULI010000120         4116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1607674,
            whole genome shotgun sequence.
ACCESSION   DASULI010000120 DASULI010000000
VERSION     DASULI010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607674"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFX34_02605"
     CDS             <1..806
                     /locus_tag="VFX34_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563827.1"
                     /translation="GYPKTLFLNTRTMREFDFQPQSLKMGRMFRENVQSPRLVGPVGW
                     KVISDSLVASGWKDGEPLLIVSPTPVYGIGLIETVLHSYVYPLRAVGVPVNEILDFEA
                     WKYNGKGFSHFLEKIFEWRPSHTIILSGDVHYSSGVTTCVKSNGSSARIVQFTSSPSK
                     NMSFSGIWGFLMKRMIAFNAWKRRGKKIRRHCDESFVIVKEEKHTSCPETPEWTEVLE
                     YVPMNKKSIVETENTIGKLTLFPNIVENTLLHMKGDTVEEFNFERVELD"
     gene            complement(905..1723)
                     /gene="kynA"
                     /locus_tag="VFX34_02610"
     CDS             complement(905..1723)
                     /gene="kynA"
                     /locus_tag="VFX34_02610"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEX5563828.1"
                     /translation="MNEQGFHIDFKENMTYGEYLQLDKILSSQNRLSGHHDEMLFIII
                     HQVSELWMKLILHELHSAIEQIEKDDLPEAFKKLARVSRIQSQIIQAWDVLSTLTPSE
                     YMEFRGSLGRASGFQSYQNRLIEFALGYKKPHIIKIYEKSPELAQELEEAYQAPSIYD
                     VTIKALARAGFVINPDILERDVSNNYTGDETVADAWLEVYRNVDQYWDLYQLAEKLVD
                     IEDCHQQWRFRHMKTVERIIGNKTGTGGSSGVNYLKSVLDHRFFSELWDVRTKL"
     gene            complement(1716..2360)
                     /gene="kynB"
                     /locus_tag="VFX34_02615"
     CDS             complement(1716..2360)
                     /gene="kynB"
                     /locus_tag="VFX34_02615"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HEX5563829.1"
                     /translation="MAGKWIDISQPLRNGMAEWPGDTKFSYEVRFAKSMTGSVNIGQL
                     ITSTHMGTHIDAPFHFDDNGLKVLDLPLDIYIGRARVIDMTGKGSIGKTDLEDVDFEE
                     AERILLKTGSRPDSMVFPETFTFLRADIGPFLKERGVKLLGIDTPSVDPETSKTMDAH
                     HSLNDNGVLILENIVLDHVSPGDYELIALPLPLADGDGSPVRAVLRPLGGQADE"
     gene            complement(2360..3631)
                     /gene="kynU"
                     /locus_tag="VFX34_02620"
     CDS             complement(2360..3631)
                     /gene="kynU"
                     /locus_tag="VFX34_02620"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX5563830.1"
                     /translation="MEKLGIDMARMRDGKDRLAPYKSEFYVSPNTIYMDGNSLGLLSK
                     RAESSVLQLLESWKLLGIDGWTEGDNPWFYLSETVGERMAPLVGGNKGEVIATGSTTV
                     NLHQLLATFYKPSGNKTKILADELNFPSDIYAMKSQLAMRGYDPKEHLVRVKSRNGRI
                     LMEDDIIDAMTDDIAIIILPGVLYRSGQILDMERLTKAAAERGIPIGFDLCHSIGSVP
                     HDLHGWGTDFAFWCTYKHLNGGPGSVGGLFVHEKHFGTAPGLAGWFGSKKETQFDMEH
                     TMDPATDAAAYQIGTPHVLSLAPVIGALEMFEEVGIDAIREKSLALTSFLLDCIKQEL
                     NQYHFEIGNPTDLTRGGHVLLEHPEAARICKALKAEGVIPDFRAPNGIRLAPVALYNS
                     FEDVWNTVQILKTIMEEERYTKFENKRGVVA"
     gene            complement(3892..>4116)
                     /locus_tag="VFX34_02625"
     CDS             complement(3892..>4116)
                     /locus_tag="VFX34_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563831.1"
                     /translation="KSIEEFLALFKGIHVKRDFSVKEGKGFKYSARINDRSVIFLHDY
                     IKIDKRYYKADDVLPEDKIEEFYEGHVSSD"
BASE COUNT         1040 a         1041 c          912 g         1123 t
ORIGIN      
        1 cggggtatcc gaagacgtta ttcctaaata ctcggacgat gcgtgaattt gatttccaac
       61 cacagtcgtt gaaaatgggg aggatgttcc gtgaaaatgt gcagagtcct cggttagtgg
      121 gccccgtcgg atggaaagtc atctcggatt cactcgtggc gtcagggtgg aaggacggcg
      181 aaccactcct cattgtgtcg cctacaccgg tatatggaat cgggttgatt gaaacggtcc
      241 tccactcgta tgtgtaccct cttcgggcag ttggggttcc tgtgaatgaa atcctcgact
      301 ttgaagcatg gaagtacaac ggcaaaggat ttagtcattt cctggagaag atttttgaat
      361 ggagaccttc ccatacgatt attttgtcgg gtgatgtcca ttattcctca ggggttacga
      421 cttgtgtaaa atcgaatgga tcatccgctc ggattgtcca attcacaagc agcccatcaa
      481 aaaatatgag cttctctgga atctggggtt ttttgatgaa acggatgatt gctttcaatg
      541 catggaagcg cagaggaaag aagattcgcc gacactgtga cgagtcgttt gtcattgtaa
      601 aagaagagaa gcatacgagt tgtccagaga cgcccgaatg gacagaagtg ctggagtatg
      661 tgccgatgaa caagaagtcc attgtggaaa cggaaaacac gatcggcaag cttacgctat
      721 tcccaaatat cgtcgaaaat actctgttgc atatgaaggg cgatacggta gaggaattta
      781 actttgaacg ggtggagctg gactgagcgg atacaccttt agcgtccaat tcctcctgcg
      841 gaccggcgaa ttctggaccg aaatagtaga accccctccc actcaacatg ggcgggggtt
      901 tcgttcaaag cttcgttcgg acatcccata gttcagagaa gaagcggtga tcaaggacgg
      961 atttcaagta gttcactccg gacgaaccac ctgttccggt tttattcccg ataatgcgtt
     1021 ctacggtttt catatggcgg aagcgccact gttggtgaca atcctcaatg tcgacgagct
     1081 tttcagccag ctggtacaag tcccaatatt gatcaacgtt gcgatacact tcgagccatg
     1141 cgtcggcgac ggtttcatct ccggtatagt tgtttgacac atcacgttcc aggatatccg
     1201 ggttaatcac gaagccggca cgtgccagtg ccttaatggt cacatcgtaa atgcttggcg
     1261 cctggtaggc ttcctctaac tcttgagcta gttctggact cttttcatag attttgataa
     1321 tgtgcggctt cttatagcca agcgcaaatt caatgagccg attttggtac gattggaatc
     1381 ccgatgccct cccaaggctc ccacgaaact ccatatattc ggacggtgtc agcgttgaca
     1441 agacatccca tgcttggatg atctgcgatt ggatgcgcga caccctcgcc agcttcttga
     1501 acgcctccgg caggtcatct ttctcaatct gttcaattgc cgaatgaagt tcgtgcagaa
     1561 tcaatttcat ccacagctcg cttacttggt ggataataat aaacagcatc tcgtcatgat
     1621 gtccggacag acgattttgg cttgatagaa tcttatccag ctgtaaatac tcgccgtatg
     1681 tcatgttctc cttgaaatca atatgaaacc cctgttcatt catctgcttg ccctccaagc
     1741 ggccgaagta ccgcgcgaac gggactgcca tctccgtcgg caagcggaag tggaagtgcg
     1801 atcaattcat aatcaccagg agacacatgg tcaaggacga tgttctctaa aatgagaacg
     1861 ccattgtcat ttagggaatg atgcgcatcc atcgttttgc tcgtttccgg atcaacggat
     1921 ggcgtgtcaa tcccaagtaa tttcacccct ctttctttca gaaatggacc gatatccgct
     1981 cgaaggaatg taaaggtttc aggaaacacc atcgaatccg gacggcttcc tgttttcaat
     2041 aaaatccgtt ccgcttcttc gaaatcaaca tcttccaaat cagtttttcc gatactccct
     2101 ttgcccgtca tatcaatgac acgcgctctt cctatataga tgtcgagcgg caaatcaaga
     2161 accttcaatc cgttgtcatc aaaatggaat ggcgcatcaa tgtgcgtccc catgtgtgta
     2221 ctcgttatca actgcccaat atttacggaa cccgtcattg atttcgcaaa tcgcacttca
     2281 tatgagaatt tcgtgtcccc tggccattct gccatgccat tccgaagtgg ctgtgaaata
     2341 tcaatccatt tccctgccat tacgcgacaa ctcctctctt gttctcgaac ttcgtatacc
     2401 gttcctcttc catgattgtt ttcaagattt ggacggtgtt ccagacatcc tcaaatgaat
     2461 tatagagcgc gacaggtgca agtcggatgc cgttcggtgc acggaaatcc ggtatgaccc
     2521 cctctgcttt cagcgcttta caaatgcgtg ctgcctccgg atgctcaaga aggacatgtc
     2581 ctccgcgcgt tagatctgtc gggttcccga tttcaaaatg atattgattc agctcttgtt
     2641 tgatacaatc cagtaagaag gatgtcaggg caagcgactt ttcgcgtatt gcatcgatcc
     2701 cgacttcctc aaacatctcc aatgcaccaa taacaggtgc caagctgagc acatgcggcg
     2761 ttccaatctg ataggccgct gcatctgtag ccgggtccat cgtatgctcc atgtcgaact
     2821 gcgtttcctt cttggatcca aaccaaccgg ctaacccagg agctgttcca aaatgttttt
     2881 catggacgaa taagccgccc acggatccag gccccccatt tagatgttta tatgtacacc
     2941 aaaatgcaaa gtccgttccc cacccatgaa ggtcatgcgg gacggaaccg atagaatggc
     3001 aaagatcaaa gccaattgga atccctcgtt ctgctgcagc ctttgtcaaa cgttccatat
     3061 cgagaatctg cccgctccgg tacaacacgc ccggtaagat aatgatggca atgtcatctg
     3121 tcattgcatc gatgatgtca tcctccataa gtatgcgtcc gttccggctt ttcacgcgaa
     3181 ccagatgttc cttcgggtcg tagccccgca tcgccagctg gctcttcatc gcatagatat
     3241 cagaaggaaa attcaattcg tccgcgagga ttttggtttt atttccggag ggcttataaa
     3301 acgtcgccaa cagttgatga aggtttacgg tcgtcgaacc tgttgcgatc acttcccctt
     3361 tgttcccacc aacaagtggc gccatccgct ccccgactgt ttcggacaaa taaaaccaag
     3421 gattgtcgcc ttccgtccag ccatcgatgc cgagtagctt ccacgattca agcaactgaa
     3481 ggacggatga ttccgctctc ttcgacaaga gccctaacga gtttccgtcc atataaatgg
     3541 tgttcggcga gacataaaac tctgatttgt aaggtgctaa ccgatctttc ccatctcgca
     3601 ttcttgccat gtcaatcccc agtttctcca ttcctcatcc tccattcaga actatctgac
     3661 tttatgagta gggtagcata tcgtactctc gaaatctcgt atgcatggtc cgaggcagaa
     3721 aaaacagccc catttcccta gaaatagagc tgttagtttt ttcgttgtaa atatcgtaat
     3781 agattatcga tcgccgaaag agaaaggatt gcaatgtttt ccgagaggat tccaacgtta
     3841 ttaggattca ccgatctttt acacataacc ttgttaccta tagaacccgt ttcaatccga
     3901 agatacatgc ccctcgtaaa actcctctat cttatcttca ggcaatacgt catctgcttt
     3961 gtaatagcgt ttgtcgattt taatgtagtc gtgcagaaag atgacactcc gatcgttgat
     4021 ccgcgcagaa tacttgaagc ctttcccttc tttcactgaa aagtcccgtt tcacatgaat
     4081 tcctttaaat aacgccaaaa attcctcaat actctt
//
LOCUS       DASULI010000121         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1209354,
            whole genome shotgun sequence.
ACCESSION   DASULI010000121 DASULI010000000
VERSION     DASULI010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209354"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFX34_02630"
     CDS             complement(<1..308)
                     /locus_tag="VFX34_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5563832.1"
                     /translation="MTKILIVDDHQLFREGVKRILDFEDSFEVVGEGDDGNEVVELYR
                     RFTPDVVLMDINMPGMNGVEATEILKNEFPDSKVIMLSIHDDESYVSHALKSGALGYM
                     L"
     gene            complement(330..1484)
                     /locus_tag="VFX34_02635"
     CDS             complement(330..1484)
                     /locus_tag="VFX34_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5563833.1"
                     /translation="MAEKTMDIQNLEKIFDNMVNVMDQSKNDIFTISEQSRQTFQEMQ
                     TEIVIIKEDISRVITEGDYLEDMTRQSRRRLADVSKNFMNYSEEQVRQAYEVANDLLV
                     RLSINRMEEKQLRVRRDELDRRIAALLETIEKADHLVNQVTTVITYLTSDLKKVGEVL
                     ETARQKQEFAIQIIQAQEEERKRLSRDIHDGPAQMLANVLLRSGLIEKTFAERGPNAA
                     LSELNQLKDMVRNALLEVRRIIYDLRPMALDDLGLIPTLRKYSSTVMEYEKGATIHFV
                     NNGTEKRFESSIEVAIFRLVQECITNALKHGKSRDVWVKVEWLRDTMNIVVKDNGKGF
                     EPSQTKDKSFGIIGMRERVELLKGEMKIMSAIGNGTTVLFRIPLHENIIE"
     gene            1630..2262
                     /locus_tag="VFX34_02640"
     CDS             1630..2262
                     /locus_tag="VFX34_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEX5563834.1"
                     /translation="MRADYKTVKLSGESEIVIQKSRFLTYVKRVETEEEAIDFIQDIK
                     KMHHTATHNCSAYIIGEHDQIQKANDDGEPSGTAGFPMLEVLKKQGLKDTAVVVTRYF
                     GGIKLGGGGLIRAYGRATTEGIAATGVVERKLHRLMKVSIDYTWLGKVENEARQSPYP
                     LEEILYEEDVSLLLYVPMEETEAFVGWMTELTNGQAEISEVSTSFLEFDL"
     gene            2427..>3219
                     /locus_tag="VFX34_02645"
     CDS             2427..>3219
                     /locus_tag="VFX34_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5563835.1"
                     /translation="MKRRDYKKMKKQTSKARLAIKIGLLVSLTALLSIAAYGLSLKQQ
                     AEQAVDRAYEAVPETNKSGVRGEAKVEPSKDNVSILLIGVDDSEARMEGNSRSDALLV
                     ATFNPEEKSVKMLSIPRDSYVYIPKVGYKDRINHAHAFGGTHAAIETVEEMLDIPIDY
                     YVKMNFNAFIEVVDALGGIEVDVPYNRLEKDENDKDTIQLVKGRHVLNGQYALALART
                     RKADSDLERGKRQQMILQAIIKEMVSVKSITKYGDVIEAVGDNMKT"
BASE COUNT          877 a          757 c          727 g          858 t
ORIGIN      
        1 agcatataac cgagcgcacc cgatttcaat gcgtgcgaca cataggattc atcatcatgg
       61 atggagagca tgatcacttt cgaatccgga aactcatttt tgaggatttc cgtcgcttcc
      121 actccattca ttccaggcat attaatatcc atcaggacga cgtccggcgt aaagcggcgg
      181 tacagttcaa ccacttcatt gccatcgtcc ccttctccca ccacttcgaa tgagtcctca
      241 aaatcaagga tccttttcac tccttcacgg aacagctggt ggtcatctac aattaaaatt
      301 ttcgtcatgt tcatttccct cccaataccc tattcaatta tgttttcatg taacggaata
      361 cggaataaca cagtggtgcc attgccaatt gccgacatga ttttcatctc gccttttagc
      421 agttcgacgc gctcccgcat ccctataatg ccaaatgatt tatctttagt ttgactcggt
      481 tcgaaccctt tgccgttatc cttaacgaca atattcattg tatcacgaag ccattcgact
      541 ttcacccaaa catctcttga ttttccatgt ttaagtgcat ttgtgataca ttcctggacg
      601 agccggaaaa tcgccacctc gatgctggac tcgaatctct tctccgttcc attgttgacg
      661 aaatggattg tggcaccttt ttcatattcc atgaccgtcg aagaatactt tcggagcgtc
      721 ggaatcaatc caaggtcatc caatgccatc gggcgcagat catagatgat ccgtcggact
      781 tcaagaaggg cattcctgac catatccttc aattggttca gctcggaaag cgcagcattc
      841 gggcctcttt cagcaaaagt cttttcaatg agccccgatc tgagcagcac gttggcaagc
      901 atttgcgcgg gtccgtcatg gatgtcgcgt gatagacgtt tacgttcctc ttcctgcgct
      961 tggatgattt gaatggcgaa ctcctgtttc tgccgggccg tttcgagaac ttctccgacc
     1021 tttttcaaat cggatgtcaa atatgtaatg accgtggtca cttggttgac aagatgatcc
     1081 gccttttcaa tcgtttccaa aagggcggcg atgcgccggt ccaactcgtc ccgccggaca
     1141 cgcaattgtt tttcttccat tcggttgata gatagcctga cgagtaaatc gttagcgact
     1201 tcgtaagctt gacgcacttg ttcctcagag tagttcatga agtttttgga aacatctgcc
     1261 aatcgtcgtc gagattggcg cgtcatatcc tccaaataat ccccctctgt gattacccgg
     1321 gaaatatcct ctttgatgat aacgatttca gtttgcatct cttggaaggt ttgacggctt
     1381 tgttcactta tagtgaagat gtcatttttg gattggtcca tcacattaac catattatcg
     1441 aatatttttt ccaagttctg gatatccatt gtcttttctg ccatctcaac ccctccctcc
     1501 tggcaagcga aaaaatctac acataggcaa tgttgcagta ttccttaatt ccttatacac
     1561 tttattatta tatcatcata aaactaattg cgtattacga ttgtattaca agtgacagga
     1621 gtgctgcaaa tgcgagcgga ttacaaaacg gtcaaattat caggtgaaag tgaaatcgtc
     1681 atccaaaagt ccagattcct cacttatgtc aagcgtgtgg agaccgagga ggaagcgatc
     1741 gacttcattc aagatatcaa aaagatgcat catactgcca cacataattg ctccgcctat
     1801 attatcggcg aacacgatca gatacagaag gcgaatgacg acggagagcc ttcaggcacg
     1861 gcaggcttcc ctatgcttga agtactaaag aagcaagggt tgaaagatac cgccgttgtc
     1921 gtgacccggt atttcggcgg aatcaaatta ggcggcggcg gactgatccg cgcatacgga
     1981 cgggccacta ccgaagggat tgccgcgaca ggcgtcgttg agcgcaagct tcaccggctc
     2041 atgaaagtgt ccatcgatta tacatggctc ggcaaagttg agaatgaagc gagacaatca
     2101 ccttacccac tcgaagagat cctctacgaa gaagacgtca gcctgctgct gtatgttcca
     2161 atggaggaaa ccgaggcatt tgtcggttgg atgacggaat tgacgaacgg gcaagcggaa
     2221 atttcagaag tatcgacttc tttccttgaa ttcgatttgt gataatagta tacgaccaag
     2281 gattttaata gtataatggt gaaagttgac gattcattgt atattctgcg gattccatac
     2341 atgtttgcgt caactttccg tttttcaagg aatattaaat tcgcttggga agattcctta
     2401 gccgatggtg gaggcaaata caagccatga aaagaagaga ttataaaaaa atgaaaaagc
     2461 aaacatccaa agcgaggctc gctattaaaa tcgggctgct ggtttcgctg actgccttgc
     2521 tttcaattgc agcttacggc ctttcgttga agcagcaagc agagcaagca gtcgatagag
     2581 cttacgaggc agttcctgaa acaaataaat ccggagtgag gggcgaggcg aaagtggagc
     2641 cttcgaagga taacgtgtcc atcctcctga tcggagtaga tgatagcgag gcgcgtatgg
     2701 aaggcaatag ccgttcagat gcccttcttg tcgctacatt caatcccgaa gagaaatcag
     2761 tgaaaatgct aagcattccg cgcgactcct atgtctatat cccgaaggtc ggctataaag
     2821 accggatcaa tcacgcccat gcgttcggcg gaacacatgc ggcaatcgaa acggtagaag
     2881 aaatgctgga catcccgatc gattattacg ttaaaatgaa cttcaatgct ttcatcgaag
     2941 tcgttgatgc acttggcggt attgaagtcg acgttccgta caaccgactt gaaaaagatg
     3001 aaaacgataa agacacgatt caactggtta aagggcggca tgtgcttaac ggccaatatg
     3061 cgctagcgtt ggcgcggact cgtaaggcgg acagcgatct cgaacgcgga aaacgccagc
     3121 agatgatttt acaggcgatc atcaaagaga tggtatcggt gaaatccatc acgaaatacg
     3181 gcgatgtcat cgaagcagtc ggcgataata tgaaaaccg
//
LOCUS       DASULI010000122         4757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_284383, whole
            genome shotgun sequence.
ACCESSION   DASULI010000122 DASULI010000000
VERSION     DASULI010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284383"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="VFX34_02650"
     CDS             <1..1604
                     /locus_tag="VFX34_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5563836.1"
                     /translation="RGKSRGTADYQMVHDEYRYAIRYELMQDLSKETITFRNHRKMRA
                     LPMRQLGSFLLGENLGGAGTHWNGHTFRFLPYDFQIKTMTDQKYGKNKLSADYLLQDW
                     GVTYDELEPYFDKFEKTAGISGENTNPFAGKRSDEYPTPPMKRTPILKKFETAASSMG
                     YHPYMLPSANLSEVYENPDGETIGACQYCGFCERFGCEYGAKTSPEITVVPTAMKTGN
                     FDVTFHANVVEVLKSGNKVTGVRYVDTQTGEEFIQPADVVVLTSYVMNNAKLLMVSKI
                     GEQYNPETGRGTLGKNYGYQILPGATGFFDEQMNVFMGAGALGMTIDDFNGDSFDHSD
                     LDFIHGASLSMTQAGVRPIQTNPIQPDVPAWGSEFKKASIENYTRVLSIGGQGASMPH
                     KENFMSLDPNYRDAYGVPLLQLTYNFTEQDRNLHKYLTDRATEIMKEMGAKTVVPGNP
                     ISDYDIVPYQTTHNTGGTIMGTDPANSVVNKYLQHWDAENLFVVGAGNFPHNGGYNPT
                     GTIGALAYRAAEAILKYSKTGGSLV"
     gene            1683..1877
                     /gene="tatA"
                     /locus_tag="VFX34_02655"
     CDS             1683..1877
                     /gene="tatA"
                     /locus_tag="VFX34_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009792201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5563837.1"
                     /translation="MSLASIGVPGLIIILVIVLILFGPRKLPEVGSAVGKTLAEFKKS
                     AREVLEDEEVKEIPKKTDIS"
     gene            1890..2756
                     /gene="tatC"
                     /locus_tag="VFX34_02660"
     CDS             1890..2756
                     /gene="tatC"
                     /locus_tag="VFX34_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5563838.1"
                     /translation="MDPYGDHNRKVSSPLDMKQVERQLETKAVEKLAMETDQEQESPL
                     ETTESDIEKMESDPTLVEHLTDLRKQLIKSAFVFMTFFIGVFATVNLWFPVVTRGHEL
                     IVLGPMEVVVFYMSISTALAFGLSLPFLCHFLWQFVKPGLNPKERQFLSLYSPVMFLL
                     FLGGLAFGYFVVNPLSYSFLVSLGAMNFKVMVSAQEYAKFLLMTTMPIGLLFELPIVA
                     MFLAAIGVLTAATLRKVRKWSYLILAVVSALITPPDFFSQLIVLIPMILLYETSIYIV
                     LHMERRNVAVNE"
     gene            complement(2795..2992)
                     /locus_tag="VFX34_02665"
     CDS             complement(2795..2992)
                     /locus_tag="VFX34_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563839.1"
                     /translation="MNKKMALAALGLGAAYLMRNKDARNKLSKQFDSFGSTPMRGEKS
                     SRTSSSTSSNRNKGLLGSLFG"
     gene            complement(3122..3262)
                     /locus_tag="VFX34_02670"
     CDS             complement(3122..3262)
                     /locus_tag="VFX34_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019381409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563840.1"
                     /translation="MNKMAIAALGLGAAFLMRNKKVRGKLMKQFESLAEPFTSKNGSN
                     LS"
     gene            3428..4477
                     /locus_tag="VFX34_02675"
     CDS             3428..4477
                     /locus_tag="VFX34_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563841.1"
                     /translation="MERDNLRLNVSLLRRRVPNLTSAAKSVGLRPATVSNLCTGKISV
                     GKSEVRTLVALADLAECSLDELIIRGEKIEMIETKIKTLDLFAPLAKGGTVGLVARKQ
                     MGQLVVMSELFYRLKSEGYTTFLLMPEGNYPELEDILENVDITTKTIDETYEKMASLG
                     VDKEIAFAADKSHVVAGGIYELQERLQQIGMDAVTTFLLDLTGEAVDEELPYGPLETL
                     WQFDADLSARHRYPAVNPIASTSSVLEGSHMDQNHFFIQQRAQKLLRRYRELRSLVAV
                     GGEEKLPPSEAQTYKRGERLEAYLTQPFYVAEEYTGQKGQSVGLQDTLKDVQAILDGS
                     FDSQDVGSLSYIGKL"
     gene            4573..>4757
                     /locus_tag="VFX34_02680"
     CDS             4573..>4757
                     /locus_tag="VFX34_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563842.1"
                     /translation="MEACRKAVEWMKDFERPWGVAGGWAIDLFLGNQTREHSDIEIAI
                     LREDQHRMKNSLADWSF"
BASE COUNT         1328 a          979 c         1213 g         1237 t
ORIGIN      
        1 aaagggggaa atcgcgtggt acagcggatt atcaaatggt gcatgatgaa tatcgctatg
       61 caatccgcta tgagctgatg caggatttgt cgaaggagac gattacgttc cgcaaccatc
      121 gcaaaatgcg tgcgcttccg atgcggcagt taggttcatt tctcctcggt gaaaatcttg
      181 gcggtgcggg gacacactgg aatggccata cgttccgctt tttgccgtac gattttcaaa
      241 ttaagacaat gaccgaccaa aagtatggga aaaacaaact ttcagcagat tatttactgc
      301 aggattgggg agtcacgtac gacgaattgg agccctactt cgataaattc gaaaagacgg
      361 cgggcatatc gggagagaat acaaatcctt tcgcgggcaa gcgttcggat gagtatccta
      421 ctcctccgat gaaacggaca ccgattctga aaaagtttga aactgccgct tcttcaatgg
      481 gctaccatcc gtacatgctg ccgtccgcca atctgtcgga agtatacgaa aacccggatg
      541 gagagacaat cggcgcttgc cagtactgcg gattctgcga acgtttcggc tgtgaatatg
      601 gggcaaagac gtcgccggaa attactgttg taccgacagc gatgaaaaca ggaaatttcg
      661 atgtgacatt ccacgcaaac gtcgttgagg tcttgaagtc cggcaataaa gtgacaggcg
      721 tgaggtacgt ggatacgcaa accggtgagg agttcatcca accagccgat gtcgtcgtgt
      781 tgacgagcta tgtgatgaat aatgcaaaat tgctcatggt ttctaaaatt ggcgagcaat
      841 ataaccccga aacaggaagg ggaacgctgg gcaaaaatta tggatatcaa attttgccgg
      901 gagcgaccgg atttttcgat gagcagatga atgtgttcat gggtgcaggt gcgctgggca
      961 tgacaatcga tgatttcaat ggcgacagtt ttgatcattc cgaccttgat ttcatccacg
     1021 gtgcgagtct atcaatgacg caagccggag tcagaccgat tcaaacaaat cctattcagc
     1081 cggatgtgcc ggcctgggga tccgaattca aaaaggcgtc cattgaaaat tatacacgag
     1141 tgctatcaat tggcggacaa ggggcttcca tgccgcataa ggaaaacttc atgtcgctcg
     1201 accctaacta tcgggatgcg tacggcgtgc cgcttttgca gttgacgtac aatttcaccg
     1261 aacaggaccg gaatttgcat aagtacctta cagaccgggc tacggaaatc atgaaggaaa
     1321 tgggcgcgaa aaccgttgtg cctggaaatc cgatctccga ctatgacata gttccttacc
     1381 aaacgacaca taatacaggt ggaacaatca tgggcacgga tcctgccaat agcgttgtga
     1441 ataaatattt gcagcattgg gacgcggaaa atctgttcgt cgtcggcgcg gggaacttcc
     1501 cgcataatgg cggttataac ccgacaggta cgattggagc attggcctat cgcgctgcgg
     1561 aagccatttt gaagtacagc aaaacggggg gttctcttgt ataagaggaa gttcagacag
     1621 ccgtaaataa aatctgagat taatttgcat gaagcgtaaa tgatagaagg gagaatgtca
     1681 ccgtgagctt agcgagcatc ggagtaccgg gcttaatcat catcttagtg atcgtcctta
     1741 ttctttttgg gccgcgtaaa ttgccggaag tcggttccgc agtcggcaag acattggcag
     1801 agttcaagaa gtctgcgcgt gaagttttgg aagatgaaga agtgaaggaa ataccgaaga
     1861 aaacggatat ttcgtaagca ggtgatgaaa tggatccgta cggagatcat aatcgcaaag
     1921 taagtagccc gctcgatatg aagcaggtgg aaaggcaact ggaaacgaag gctgtggaaa
     1981 agcttgctat ggagacggac caagagcaag aaagcccgct tgaaacgacg gagagcgaca
     2041 ttgagaagat ggaaagtgat ccaacacttg tcgagcactt gactgatctg cgcaaacagc
     2101 tcattaaaag tgccttcgta ttcatgacat tttttatcgg tgtctttgcg accgtcaatc
     2161 tatggtttcc agttgtcacg agagggcatg aattgattgt cctcggacca atggaagttg
     2221 tcgtcttcta catgtcgatt tcgacggcat tggcatttgg attgtcgctt ccgttcctct
     2281 gtcatttctt atggcaattc gtgaagccgg gactgaaccc gaaggaaagg cagtttctaa
     2341 gcctgtattc accggtcatg ttcctattgt tcctaggagg cttagcattc ggctacttcg
     2401 tcgttaatcc gttaagttac agcttccttg tatcgttagg tgcgatgaac ttcaaggtaa
     2461 tggtgtctgc acaggaatat gcaaagttcc tgctcatgac aacgatgccg attggactct
     2521 tattcgagct gccgatcgtt gccatgtttc ttgccgcaat cggcgtgttg accgctgcca
     2581 cattacggaa agtgagaaag tggtcctatc tcattctggc agttgtttca gcgctcatta
     2641 caccacccga tttcttcagt cagcttatcg tcctgattcc gatgatttta ctgtatgaaa
     2701 cgagcattta catcgttctt cacatggaaa ggcgaaatgt tgcagtgaat gaataaaagg
     2761 agaggacgcc atcttatgcg tcctctcctt tctttcagcc gaacaaactg cccagcaatc
     2821 ctttatttcg attggatgaa gtggaactgg aagtgcggga gcttttctcg cctctcatcg
     2881 gcgttgagcc gaatgaatca aactgcttcg ataatttatt tcgtgcgtcc ttgtttctca
     2941 tcaaatatgc tgcgcctaaa ccgagtgcgg ccaatgccat ttttttattc atcatcgtaa
     3001 cctcctgatt ttttgcttat caaatacttt cccgatttga aagagactaa acaaaaggaa
     3061 atccatattc aaaaaaaatc ggcacccata aaggatgccg attttcccgt gagaaagatt
     3121 tttatgaaag atttgatcca ttttttgagg tgaatggttc tgcgagcgat tcaaactgct
     3181 tcatcagctt tcctcggact tttttattcc gcatcagaaa agccgctccg agtcctaatg
     3241 ccgctatcgc cattttattc ataaaaacgt caccttcctc acattgaatt ttggatcatt
     3301 gttacgatgc cctatttcgt attttctatc cgttctttag tactgaaaaa taaattacgt
     3361 tgtagagtaa attcttattg cattacagca taaaataaat tactatacaa agcaaggggt
     3421 gagaaaattg gaaagagaca atcttcggtt gaatgtttca ttgttgagga gaagggtgcc
     3481 taatctgacg agtgcagcta aatcagtagg tcttcggcca gcgacagtgt cgaatctgtg
     3541 caccggtaag atttcggtcg gcaaatcgga agtgaggacg cttgtagcgc tagcggattt
     3601 ggcggaatgc agtttggacg aattgataat ccggggggag aagattgaga tgatcgaaac
     3661 gaagatcaag acgttggatt tatttgcacc attggcaaaa ggcgggacag tcggcctggt
     3721 cgctcggaaa caaatgggtc agctggtcgt catgagtgaa ttattctacc gattgaagag
     3781 tgaaggctat acgacattcc tattaatgcc cgaggggaat taccctgagt tggaagatat
     3841 tttggaaaac gtggatatta caacgaaaac gattgacgaa acgtatgaga aaatggcatc
     3901 tctcggcgtt gataaagaaa ttgcatttgc tgcagacaaa agccatgtag tggctggtgg
     3961 aatttacgag ttgcaagagc gcttgcagca aatcggaatg gacgccgtaa cgaccttcct
     4021 gttggattta acgggagaag cggtagatga agaactgcca tatggcccgc ttgaaacgct
     4081 ttggcaattt gacgctgatt tgtcggcaag acaccgatat cctgcagtca atccgattgc
     4141 ctccacttca tctgttctgg aaggctcaca catggatcag aatcatttct tcattcagca
     4201 aagagcacaa aaattattaa gacgttaccg ggaattgcgt tcacttgttg ctgtaggagg
     4261 agaagagaaa ctgccgccat ccgaagcgca aacgtataaa cgtggagaac gtttggaagc
     4321 gtatttgacc cagccattct atgttgctga agaatatacc gggcaaaagg gccaatccgt
     4381 gggtcttcaa gatacattga aagatgtgca agccatattg gacggctcat ttgattcgca
     4441 agatgtagga agtctaagct atatcggaaa attgtaataa cagtttgtga gaattccgat
     4501 gttcagataa aatgaaaaga caacaaagga gtgacgcggt cgctcctttt ttcgtgtata
     4561 gggggaatga tcttggaggc gtgtcgaaaa gcggtggaat ggatgaagga tttcgaacga
     4621 ccgtggggag ttgcgggcgg ttgggcgatt gacctttttc ttgggaatca aacaagggag
     4681 cattcagata ttgaaatcgc tatccttcgg gaagatcagc atcggatgaa aaatagcttg
     4741 gctgactggt cctttca
//
LOCUS       DASULI010000123         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_890763, whole
            genome shotgun sequence.
ACCESSION   DASULI010000123 DASULI010000000
VERSION     DASULI010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890763"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1403)
                     /locus_tag="VFX34_02685"
     CDS             complement(258..1403)
                     /locus_tag="VFX34_02685"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX5563843.1"
                     /translation="MSTLFNNYARRPVHLVKGKGTVVEDANGKKYLDFTSGIAVVSLG
                     HAHPAIVNAIQAQSEKLWHISNLFESPEQEKLAASLMEDTHFSHSFFCNSGAEANEAA
                     IKLARKHTGKSTIITFDQSFHGRTFGAMSATGQEKIKQGFGPMLETFKTVPFNDPVKL
                     DAAIDDDVAAIMLEVIQGEGGIHAVSPEFASVISDICQSKGILCIVDEVQTGIGRTGT
                     RYAYEQAGLKPDILTLAKGLGGGFPIGAMLGTAELFDSFGPGTHGTTFGGNPLAVAVA
                     QTVVDYVFDVDFLKVVRHKSNALTQKLKESLPSERFEIRGQGLLIGIDCKEDVAPFIR
                     QAEEAGLLVVQAGSNVIRLLPPLTVTEQEIDQALDILTSILLVPSTL"
     gene            complement(1400..2179)
                     /gene="argB"
                     /locus_tag="VFX34_02690"
     CDS             complement(1400..2179)
                     /gene="argB"
                     /locus_tag="VFX34_02690"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5563844.1"
                     /translation="MTTSKSMLHTDHKRIIIKLGGSMLEGLQSGFPKKLHEMQREGYE
                     IVIVHGGGPSINAALQKNAIETNIVNGIRVTCEKSIAIVKDVLIGDVNPSLVHQLNRE
                     GIEAIGLSGFDGKLLTCTLLDEERFGLVGDIQAVNKRLLEKLLAAGIVPIVSCIGATD
                     CGKPLNINADTVASKVALAIGASSLMFVTDTPGIKIGNDIQSTVSPSEIETWIEAGDI
                     YGGMIPKVHAAIACLDAGVPSVQIVNQYLEGTIIGLEEVLN"
     gene            complement(2142..>3041)
                     /gene="argJ"
                     /locus_tag="VFX34_02695"
     CDS             complement(2142..>3041)
                     /gene="argJ"
                     /locus_tag="VFX34_02695"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX5563845.1"
                     /translation="GIAPNLVGVASTGVIGEKMNMAPVLKGIEQLKPTSTLEGSIDFS
                     QAILTTDTVMKNVTYRTEVDGKEVIIAGTAKGSGMIEPNMATMLGFITTDAKIDSGHL
                     QEALKSITDVTFNSITVDGDTSTNDMVLVLANGMADNQPLSPAHPDWNPFIQTLHAVA
                     QDLAKMIAKDGEGATKLIEVEVDGAVTDVEARKIAKTVVGSPLVKTAVFGCDANWGRV
                     IAAVGYSGATIDPLGITIKIGDTPVVKDGEPVIFSEEQLLIYLKQPEVKIFVGLNKGS
                     GHGIAWGCDLTYDYVQINASYRS"
BASE COUNT          688 a          828 c          687 g          838 t
ORIGIN      
        1 cggaccgatt atcgggccaa ccgatgtcaa aggtgagcga agtgtcgacg cgatgaaaat
       61 aatgccgatg atcaataacc attttttctt ttcaagccta tgtattgttg agttgctttg
      121 attttcctca aacttcatcg tatcgcctcc cttttcgtat acatgcccta tgagcatacc
      181 acattgggca ttctatcaga ttaacggcaa aaacggatct ccttgttacg tgagatccgt
      241 ttttttcgat aaataactta tagagtcgac ggtacaagca aaatggatgt caggatgtca
      301 agagcttgat cgatttcctg ttcagtcact gtcaacggag gcagtagtcg gatcacattc
      361 gaaccggcct gcacaacgag tagccccgcc tcctccgctt ggcggatgaa aggcgcgacg
      421 tcttccttgc aatcgattcc gataagcaaa ccttggcctc ggatttcgaa tcgttcagac
      481 ggcaggcttt ccttcaactt ttgagtcaaa gcattggatt tatgacggac gaccttcagg
      541 aaatccacgt cgaaaacgta atccactacg gtttgggcaa ctgcgacagc tagggggttc
      601 ccgccgaatg ttgtgccatg cgtcccgggg ccgaatgaat cgaacagttc ggctgttccg
      661 agcatcgccc caatcgggaa gcccccgccc aatccttttg ccagtgtcag tatatccggt
      721 ttcaatcccg cctgctcgta tgcataacgg gtgccggtcc gtccgatgcc ggtctgcact
      781 tcatcgacaa tgcataaaat tcccttcgac tggcaaatgt ccgaaatgac tgaagcgaac
      841 tccggcgaga ctgcatggat gcccccttcg ccttggatca cttccagcat gatagccgcc
      901 acatcgtcat cgatagcggc gtccagtttc acaggatcat tgaaaggcac cgtcttgaac
      961 gtttcaagca tcgggccgaa tccttgtttg atcttctctt ggcccgttgc cgacatcgct
     1021 ccgaatgtgc ggccatggaa tgattggtcg aacgtgatga tcgtcgattt ccctgtatgc
     1081 tttcttgcta gcttgatagc cgcttcattc gcttccgccc cgctgttgca gaagaagcta
     1141 tgggagaaat gcgtgtcttc cattaaggaa gctgcaagct tttcctgctc gggactttcg
     1201 aataaattcg atatatgcca tagcttttca ctttgagcct gaattgcatt gacgattgcc
     1261 ggatgcgcat ggcctaaact gacgacagcg atgccgcttg taaaatcgag gtacttcttg
     1321 ccattcgcat cttcaacgac cgttcccttg ccttttacaa ggtgtaccgg tcttcgcgcg
     1381 tagttgttga ataacgtact caattgagca cctcctcgag tccgataatg gtaccttcca
     1441 aatactgatt gacaatttgg acggatggaa cccctgcatc taggcaggct atcgcagcat
     1501 gcactttcgg aatcatgcct ccgtatatgt cccctgcttc tatccaggtc tctatttcgc
     1561 taggcgaaac ggtcgattga atatcatttc cgatttttat tcccggtgta tccgtcacaa
     1621 acatgagact tgacgcccca attgcgagtg cgactttgct tgcgactgta tctgcattta
     1681 tattgagcgg ctttccgcaa tctgttgcgc cgatacacga gacgatcgga acgattccgg
     1741 ctgccagcaa tttttcaagc aggcgtttat tgactgcttg gatgtcaccg acgagaccga
     1801 atctttcttc atcgagcaat gtacacgtca atagcttgcc gtcgaaaccg ctaagcccga
     1861 tcgcttcgat gccttcacga ttcaattggt gaacgagtga tggattcacg tcgccgatca
     1921 acacatcctt cacgatggcg atggactttt cacaagtgac ccggatgcca ttgacgatgt
     1981 tggtctcaat ggcatttttc tgcaaagcag catttatgga aggtccgccg ccatgaacga
     2041 tgacaatctc atagccttcc cgttgcattt cgtggagttt cttaggaaaa ccgctttgca
     2101 atccttcgag catgctgccc ccgagtttaa tgatgatccg tttatgatct gtatgaagca
     2161 ttgatttgga cgtagtcata tgtcaaatca cacccccacg ctattccatg gccgcttcct
     2221 ttattcagcc cgacaaagat cttcacttca ggctgtttta aataaatcag cagctgctct
     2281 tccgaaaaga tgaccggttc tccgtccttc acgacaggcg tgtcgccgat tttaatcgtg
     2341 atgcctagcg gatcgatggt tgcgccgcta taccctacag ctgcaatgac cctcccccag
     2401 ttcgcgtcac agccgaacac tgccgttttg acgagaggag acccaacgac cgtcttggcg
     2461 atttttctcg cttccacatc cgttactgca ccgtccactt ccacttcgat aagcttcgtc
     2521 gccccttccc catccttagc aatcattttt gcaagatctt gggcgaccgc atgcaatgtc
     2581 tggatgaatg gattccagtc cgggtgggcc ggcgataatg gttgattgtc cgccatgccg
     2641 ttcgccagca caaggaccat gtcgttcgtg gaggtatccc catctaccgt aatcgagttg
     2701 aacgtcacgt cagtaataga cttcaacgct tcctgcaaat gacctgaatc gattttggca
     2761 tcggtcgtta tgaagccgag catcgtcgcc atattcggtt caatcatccc cgaacctttc
     2821 gctgttcccg cgatgatgac ttccttgcca tccacttccg tacgatatgt cacgtttttc
     2881 ataacagtat ctgtcgtcaa aatcgcttga gaaaaatcga tagatccttc aagggtactt
     2941 gtcggcttca gctgctcaat ccctttcagc acaggtgcca tattcatttt ctcgccgatg
     3001 acacctgttg acgctactcc gaccaagttg ggtgcaatgc c
//
LOCUS       DASULI010000124         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1557577,
            whole genome shotgun sequence.
ACCESSION   DASULI010000124 DASULI010000000
VERSION     DASULI010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557577"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFX34_02700"
     CDS             complement(<1..420)
                     /locus_tag="VFX34_02700"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5563846.1"
                     /translation="MNLPTAFHEKIINCFGEAGENWLGTLEETLNGIADTWGLTLESP
                     VTNLSYNYVIHVTDQNNDPYILKMGLPGFDFNNEIRTLQLYNGQGCARLVRADEERGA
                     MLLEKLQPGTMLCIETDEETAIQTFSHVWKQIRRPLPK"
     gene            complement(455..961)
                     /locus_tag="VFX34_02705"
     CDS             complement(455..961)
                     /locus_tag="VFX34_02705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5563847.1"
                     /translation="MRVREALPGDAEGIARVHVDSWRTTYKGIVSESVLQNLSTEQRA
                     ENWRRGIGKNPLYVAEDESGRIVGFATGGKERTGNYEADGELYAIYLLHEVHGKGIGK
                     KLTQLIAQNLKEQGFTSMLVWVLEQNPSKKFYESLGGQPVDEAMIEIGEEDFKEIAYY
                     WDNIETLA"
     gene            complement(1182..2585)
                     /locus_tag="VFX34_02710"
     CDS             complement(1182..2585)
                     /locus_tag="VFX34_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX5563848.1"
                     /translation="MTEHKALQYAVEEITEIAKGFGLDFYPMRYEICPADIIYTFGAY
                     GMPTRFSHWSFGKQFHKMKLQYDLGLSQIYELVINSDPCYAFLLESNTLIQNKLIIAH
                     VLAHCDFFKNNSRFANTRRDMVESMTATAERIADYEMLYGNDEVERFLDAVLAIQEHV
                     DPSLIRWRHGNEEVEQEDEVPIVRKTEYDDLWGMDHKRLKPPAETKRRKTPPAPEKDL
                     LLFILENSRELEEWQRDILTMIREEMLYFWPQMETKIMNEGWATYWHQRIIREMDLTT
                     DETIEFAKLNANVIQPSRTSINPYYLGLKIFEDIERRYDHPTEEMIELGVNPGSGREK
                     MFEVREMDSDISFIRNYLTKDLVQKEDLYVFEKKGSHYKISDKEYRNVQNRLIAQRVN
                     GGFPYIVVHDGDYVRNGELYLVHRYEETELDVPYLENVLPYIHQLWGRIVHLETYVDN
                     KQVVFSYDGKKVHRRFA"
     gene            complement(2582..>2962)
                     /locus_tag="VFX34_02715"
     CDS             complement(2582..>2962)
                     /locus_tag="VFX34_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX5563849.1"
                     /translation="KVVTEVDFFSKGESGGTICSTAYEKALDLIAQKYNPSRYNIYPF
                     HFSDGENMSSDNGKCMSLVEELMEVSNMFGYGEVNAHSRYSTLMNTYKKIEDPKFRHY
                     VLKENKDVYYALKKFFHKRLEGSV"
BASE COUNT          706 a          772 c          573 g          911 t
ORIGIN      
        1 tttcggcaat ggccgacgga tctgtttcca tacatgagaa aatgtttgta tggcagtttc
       61 ctcatctgtt tctatgcaga gcatcgtccc gggttgaagc ttctcaagca gcatggcccc
      121 ccgctcctcg tcggctctca ctaatcttgc acacccttgc ccattgtaga gctgcaatgt
      181 ccggatttca ttgttgaaat cgaatcccgg caagcccatt ttcaatatgt aaggatcgtt
      241 gttttgatcg gttacgtgaa tgacgtagtt ataggaaaga ttcgtaacag ggctttccaa
      301 tgtcaatccc cacgtatctg caatgccgtt tagagtttct tccaatgttc ctagccaatt
      361 ctctcccgct tcaccgaaac agtttattat tttttcatgg aatgcagttg gtaagttcac
      421 actggatcat cttccttcat taataaataa attcttatgc taatgtttca atgttgtccc
      481 agtaataagc aatctccttg aaatcctctt ccccaatttc gatcatcgcc tcatcgacag
      541 gttgtccgcc gagcgattca tagaatttct tcgatggatt ttgctccaat acccatacga
      601 gcattgacgt gaatccttgt tctttcaggt tttgtgcaat taattgtgtc agcttcttgc
      661 cgatcccctt gccatgtact tcatgtaata aataaattgc gtataattcc ccatccgcct
      721 catagttgcc ggtccgttct ttgccccctg ttgcgaaacc gacaattctt ccgctttcgt
      781 cttcagcaac atataaaggg tttttcccaa ttcctctacg ccaattttcg gcacgctgtt
      841 cagttgataa attttgaaga acggactcgg aaacgattcc cttatacgtt gtcctccaac
      901 tatcgacatg tacgcgtgcg attccttctg catcgcctgg tagggcctct ctgactctca
      961 tctatcccac ccctttttcc tttagtttac agtaaatggt gaaataatag aatcggtttt
     1021 acaaatagtc tttggaattt ggagtcgtaa cgatatatcg atcatttccc aggttttatc
     1081 gctcatttcc caagatatat tgtcatttcg tgactaagga cccgcctcca acacaccaaa
     1141 aagccgccac attgcaaccc aatgcggcgg ccacacactc cctatgcaaa tctccgatga
     1201 actttcttgc catcgtatga aaacacgact tgtttattat ccacatatgt ttccaaatgc
     1261 acgattcggc cccatagttg gtggatatat gggaggacat tctccaaata cgggacatcc
     1321 agctcggtct cttcatatct atgcaccaaa tagagttccc cgttgcggac gtaatcgccg
     1381 tcatgcacga caatataagg gaagcccccg ttcacccgct gcgcgatgag gcggttctgc
     1441 acattccgat attccttatc cgatatttta taatggcttc cttttttctc gaacacatat
     1501 aaatcttcct tttgaacgag gtctttcgtc aaatagttgc ggatgaagga aatgtccgaa
     1561 tccatctccc tcacttcgaa cattttctcg cggccggacc ctggattcac tcctaattcg
     1621 atcatctcct ccgtcggatg atcgtatctc cgttcgatgt cctcgaagat tttcaagcct
     1681 aaataatacg ggttgatgga cgttctggaa ggctggatga catttgcgtt gagcttcgca
     1741 aactcgatcg tttcatctgt cgtcaaatcc atttccctaa taatccgttg atgccagtaa
     1801 gtcgcccatc cctcattcat gatctttgtt tccatttgcg gccagaaata aagcatttct
     1861 tcccggatca tcgtcaatat atcccgttgc cactcttcta attccctgct attttccaag
     1921 atgaaaagca gcagatcctt ttcaggggca ggaggcgtct tcctacgttt cgtttccgcc
     1981 ggcggcttca gccttttatg gtccattccc catagatcat cgtattccgt tttgcggaca
     2041 atcggcactt cgtcttcctg ctcgacttct tcatttccat gacgccatct gataagggac
     2101 gggtcgacat gctcctggat ggctagcacc gcgtccaaaa agcgctcgac ctcatcattt
     2161 ccataaagca tttcataatc ggcaatcctc tcggctgtag ccgtcatgct ttcgaccata
     2221 tcccgcctcg tgttcgcgaa gcgggaatta tttttaaaga aatcgcagtg cgcaagaaca
     2281 tgcgcgatga tcagcttgtt ctggatcagc gtattacttt ccagcagaaa agcgtaacaa
     2341 ggatccgaat tgatgacgag ctcgtatatt tgactgaggc ccaaatcata ttgcagcttc
     2401 attttatgga actgtttgcc gaagctccaa tgtgaaaacc gtgtcggcat tccgtatgcg
     2461 ccgaatgtat aaatgatatc agcgggacat atttcatagc gcatcggata aaaatccaag
     2521 ccgaatcctt ttgcaatctc cgtgatttcc tccacggcat attgtagcgc cttgtgctcc
     2581 gtcatacgga cccctccagg cgtttatgga aaaacttctt caatgcatag tacacatcct
     2641 tgttttcctt cagcacataa tggcggaatt tcgggtcttc gattttttta tacgtattca
     2701 tcagtgtcga atagcggcta tgcgcattca cttccccgta gccgaacata ttcgacactt
     2761 ccatgagctc ttccactaac gacatgcatt ttccgttatc cgaggacata ttttccccat
     2821 cagagaaatg gaaaggatat atattatagc gtgatggatt gtatttctgt gcaatgagat
     2881 cgagcgcttt ttcataggcg gtcgaacaaa tcgtgcctcc gctctcccct ttcgagaaga
     2941 agtcgacttc cgtgacgact tt
//
LOCUS       DASULI010000125         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1616703,
            whole genome shotgun sequence.
ACCESSION   DASULI010000125 DASULI010000000
VERSION     DASULI010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616703"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFX34_02720"
     CDS             <1..624
                     /locus_tag="VFX34_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5563850.1"
                     /translation="GALSVIAASGIKLRAFSKLSGVLFLAMIPVTGILYLFRNTIITD
                     GRKGRILSFLNPFDYAQGSGYQIANGYIAIGAGGIKGLGLGNSIQKMGYLPEPQTDVI
                     MAVISEETGVFGTVIVIGGLGFIVMRALYIAMRTKDPQARMLAAGIGSVIGIQTFVNI
                     GGLTGLIPLTGVPLPFISYGGTSMILLSLSLGILMNVSMFVKYQKNK"
     gene            667..>2037
                     /locus_tag="VFX34_02725"
     CDS             667..>2037
                     /locus_tag="VFX34_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5563851.1"
                     /translation="MLVANRGEIAIRIFRACTELEIPTVGIYSAEDRGSFHRYKADES
                     YLVGEGKKPIDAYLDIEGIIKIAKSSGANAVHPGYGFLAENEVFARRLEEEGIIFIGP
                     KSKHLDMFGDKVKAREQAVKAGIPVIPGTDGPVSSVAEVASFGEKYGFPIIIKASLGG
                     GGRGMRIVNSADEVLEAYERARSEAKSAFGSDEVYVEKYINKPKHIEVQILGDTYGNV
                     VHLYERDCSIQRRHQKVVEIAPSISLDDKLRNEICEAAVKLAKNISYINAGTVEFLVA
                     DGSFYFIEVNPRIQVEHTITEMVTGIDIVHAQIYIANGGNLHEGEAAIPKQEDIPLFG
                     YAIQSRVTTEDPLNDFMPDTGKLMVYRSGGGFGVRLDAGNGFQGAVITPYYDSLLVKV
                     STWGTSFRDAAAKMDRNLQEFRIRGIKTNIPFLENVVRHESFLTGNYDTSFIDSTPEL
                     FEFPVRK"
BASE COUNT          564 a          409 c          529 g          535 t
ORIGIN      
        1 ggagctctgt ctgtcattgc agcgagcggc ataaaattga gggctttctc gaaattgagc
       61 ggtgttttat ttctagctat gattcctgtt acaggaatac tttatttgtt tcgaaacaca
      121 atcattacgg atggaaggaa aggacggatt ttgtcgttct tgaacccgtt tgattatgca
      181 caaggctctg gctatcaaat tgcaaatgga tatatcgcaa ttggagcagg tggcatcaaa
      241 ggtctcggtc taggaaactc aattcaaaaa atgggatact tgccagaacc acaaactgat
      301 gtcatcatgg ctgtcatctc ggaagaaacc ggtgtttttg gaacagtgat tgtcatcggc
      361 gggcttggat ttatcgtaat gcgcgccctt tatattgcaa tgcggacgaa ggatccgcag
      421 gctcggatgc tggctgcggg gatcgggagt gtcatcggca tccaaacttt tgtcaacatc
      481 ggaggactga caggacttat tcctcttacg ggagttccgt tgccttttat tagttatggc
      541 ggaacttcga tgattctgtt atctttatct ttaggaatat tgatgaatgt gtcaatgttc
      601 gtgaaatatc aaaagaataa atagaaaagg aagtgctttc atggtggaat tgggtaaaat
      661 aaaaaaattc tagtagcgaa ccgcggtgaa atcgccattc gcatttttcg cgcgtgcact
      721 gaattggaga taccgactgt cggaatttat tcggcagaag ataggggatc tttccaccgt
      781 tacaaagcag atgaatctta tctagtcgga gaaggaaaaa aaccgatcga tgcttatttg
      841 gatatcgaag ggatcatcaa gattgcaaaa tcatcggggg caaatgctgt ccatcccgga
      901 tatggcttcc ttgctgaaaa cgaggtgttt gcacgcaggc ttgaagaaga aggcatcatc
      961 tttatcggac cgaagtcgaa gcatcttgat atgttcggag acaaggtgaa ggccagggag
     1021 caagcggtca aggcgggcat cccagtcata cctggcacag atgggcctgt ttcgtctgtg
     1081 gcggaagtcg cctcgtttgg agagaaatac gggtttccca tcatcatcaa agcttcttta
     1141 ggcggtggag gcaggggaat gcggattgtg aattccgcag acgaggtgct tgaagcatat
     1201 gaacgcgcaa gatctgaagc taaatcggcg tttggttcag atgaagtata tgtagagaaa
     1261 tacattaata agccaaaaca tattgaagtg caaattttag gtgatacata cggcaatgtc
     1321 gtgcacttgt atgaacgcga ttgttcgatt caacgccgtc accagaaagt ggtggaaatc
     1381 gccccatcca tatcattgga cgacaagctg cgcaatgaaa tttgcgaggc ggccgtcaag
     1441 ttggcaaaaa acatttcgta tatcaatgca ggaactgttg aatttctcgt tgcagacgga
     1501 agtttttatt tcatcgaagt gaatccgcgc attcaagtcg agcatacgat cactgaaatg
     1561 gtgacaggca tcgatatcgt ccatgcgcag atttatatcg ccaatggcgg aaaccttcat
     1621 gaaggagaag cggccatacc taaacaggaa gatattcctc tgttcgggta cgcaatccaa
     1681 tcgagggtga caacggaaga tccattgaat gatttcatgc ccgacaccgg caagctgatg
     1741 gtttaccggt cgggtggggg gttcggcgtc cgcctcgacg cggggaatgg cttccaaggt
     1801 gccgtaatca ccccatacta tgattccctt ctcgtcaaag tttcgacttg gggaacttca
     1861 ttcagggatg ccgctgcaaa aatggaccgg aaccttcagg aattccgcat taggggaatc
     1921 aagacgaata ttccattctt agaaaatgtg gtccgtcatg aaagcttcct gactggcaat
     1981 tatgatacaa gcttcatcga ttcgacgcct gaattattcg agttcccggt taggaaa
//
LOCUS       DASULI010000126         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_418291, whole
            genome shotgun sequence.
ACCESSION   DASULI010000126 DASULI010000000
VERSION     DASULI010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418291"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="mazG"
                     /locus_tag="VFX34_02730"
     CDS             <1..968
                     /gene="mazG"
                     /locus_tag="VFX34_02730"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX5563852.1"
                     /translation="FIASEVKLSLMEKYAYDHPVTIVTAAGSSLEKLRTVPLFELDRE
                     TEIDNLTTVYVPPVMEREGRLKEWSTFREIIAALRAPDGCPWDREQTHESLKRYLIEE
                     AHELLEAIDQEDDEGIIEELGDVLLQVFLHAQIGEDDGYFAMEDVLQAIGDKMIRRHP
                     HVFGDRDVENSDEVLKNWQEIKKEEKPQAESLLDGQARHSSSLLTSFNYQKEAAKVGF
                     DWPDITGAFDKFEEEWQEFKDEVENGTKGSQTDELGDVLFTLVNLSRFLKLSPEEAMA
                     HANEKFKRRFSFVEQSVKNGRGEFSAYTLDELEGFWQQAKGEEKK"
     gene            965..1255
                     /locus_tag="VFX34_02735"
     CDS             965..1255
                     /locus_tag="VFX34_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX5563853.1"
                     /translation="MRLDKFLKVSRLIKRRTLAKQVADQGRITINGKVSKASSVVKPG
                     DELAIRFGQKIVTAKVEMLKESTKKEDAAMMYTIVKEEKLEKVEPEFIDDED"
     gene            1491..1787
                     /gene="yabP"
                     /locus_tag="VFX34_02740"
     CDS             1491..1787
                     /gene="yabP"
                     /locus_tag="VFX34_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HEX5563854.1"
                     /translation="MQIQTDNPSYAIPSGDHVVTMRNRKRMDLTSVKTIDRFDQEEFI
                     VRTSQGVLQIRGEELRIVHLDVDKGLLTLEGTIGTMQYDEEDSGSRNFLHKLFG"
     gene            1784..2251
                     /gene="yabQ"
                     /locus_tag="VFX34_02745"
     CDS             1784..2251
                     /gene="yabQ"
                     /locus_tag="VFX34_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HEX5563855.1"
                     /translation="MSLSVQFLSLLAMIGTGIGAGVFMDLIGTGVDATGKKSIIRKYA
                     VPLEVIGWILIGCASFYVLFIVRDGAWRMYDPVAQICGMLLYASIFHRPFRFFGRILN
                     MLIIKPIWFIIMLIVKIISYIYRLIVKIVGFLLSPFVFLYHKVYNSLFKSDKQ"
     gene            2307..2723
                     /locus_tag="VFX34_02750"
     CDS             2307..2723
                     /locus_tag="VFX34_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5563856.1"
                     /translation="MEQRKRKSSTTVASIETEYVRSLRKKEVWRNKQKKRLQKKLFIY
                     AVIAFVVLGGLTNMYIQQQQTLKEKEQQKVETLAKLEEVREEQELLKKQLVKLDDDDY
                     IAKLARKEYFLSDENEIIFSVPENKKKKDEKDDGKE"
     gene            2848..3267
                     /locus_tag="VFX34_02755"
     CDS             2848..3267
                     /locus_tag="VFX34_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HEX5563857.1"
                     /translation="MSIEVGSKLQGKVTGITNFGAFVELPNGSTGLVHISEVADNYVK
                     DINDHLKVGDMVEVKVMNVGTDGKIGLSIRKAKPEAERPQRPQRPRHSGGRPSHNDRP
                     ENFEQKMAKFMKDSEERLSTLKRATESKRGGRGARRG"
     gene            3576..4019
                     /locus_tag="VFX34_02760"
     CDS             3576..4019
                     /locus_tag="VFX34_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5563858.1"
                     /translation="MNEILSNLRKHVEWAESLHTLSEEQADTPYQEGKWTPKEILMHM
                     AEWDRFTLEHRLHRLKESEKLEDVEWGPFNEQAAKLSKEYSFDEVIALAKQHREQIIE
                     WLEGLDETEWTKPFNIGEHVLTLEDYFSDFVWHDSHHKEQIESIR"
     gene            complement(4243..4316)
                     /locus_tag="VFX34_02765"
     tRNA            complement(4243..4316)
                     /locus_tag="VFX34_02765"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4280..4282),aa:Met,seq:cat)
     gene            complement(4320..4394)
                     /locus_tag="VFX34_02770"
     tRNA            complement(4320..4394)
                     /locus_tag="VFX34_02770"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4360..4362),aa:Asn,seq:gtt)
BASE COUNT         1306 a          831 c         1191 g         1116 t
ORIGIN      
        1 cgtttatcgc ttctgaagta aagctctcgc taatggagaa gtatgcatat gaccacccgg
       61 tgacgattgt gacagcggcg ggttcttcat tggaaaagct tcggactgtg ccgttatttg
      121 aattggaccg tgagacggaa atcgataatt tgacgacggt ttatgtacct cctgttatgg
      181 agcgtgaagg gcgtttgaag gaatggtcga cattccggga aattatcgcc gcattgcgtg
      241 ctccggacgg ctgcccatgg gaccgcgaac agacgcatga atcgctgaag cggtatttga
      301 tcgaggaagc gcatgagtta ttggaagcga ttgaccagga ggatgatgaa ggcatcatcg
      361 aagaattggg cgacgtgctt ctgcaagtgt tcttacatgc gcaaatcggt gaggacgatg
      421 gctattttgc aatggaagat gtattacagg caatcggaga taaaatgatc cgccgccatc
      481 cgcatgtgtt cggcgatcgg gatgtagaga attcggatga ggttttgaag aattggcagg
      541 aaatcaagaa ggaagagaag ccgcaagccg aatcgctgtt ggatggacag gctcgccata
      601 gttcgtcgct gctcacttca ttcaattatc aaaaagaagc ggcgaaagtt ggtttcgatt
      661 ggccggatat aacaggcgct ttcgataaat ttgaggaaga atggcaggaa ttcaaggacg
      721 aggtggagaa tggaacgaaa gggagccaga cggatgagct cggcgacgtc ctctttacat
      781 tggtcaatct gtcgaggttc ttgaagctgt ctcctgaaga agcgatggcg catgcaaacg
      841 agaaattcaa acggcgcttt tcgtttgtcg agcaaagcgt aaagaacggc agaggcgaat
      901 tctccgctta tacgcttgac gaactcgaag gattttggca acaggcgaag ggggaagaaa
      961 agaaatgaga ttggataaat ttttgaaagt atcacgcttg attaaacggc ggacattggc
     1021 gaaacaagtc gccgaccaag gccggatcac gattaacggt aaggtatcga aagcatcgtc
     1081 ggtcgtgaaa ccgggcgacg aacttgctat tcgtttcggt caaaaaatcg ttactgcaaa
     1141 ggttgaaatg ttgaaggagt cgacgaaaaa ggaagacgct gcaatgatgt acacgattgt
     1201 gaaagaagag aagttggaaa aggtcgaacc tgagtttatt gatgatgaag attgagtgaa
     1261 atataccgta atagggattt cctcagcttg attgaaaacg cttgttagcc taggcgcgaa
     1321 gtcgagcgaa gacgcgaata gaactaaagt tcatgagcgg acgagcgagg cgagcaacaa
     1381 cggatgcgag cgtttgtcaa caggctgaca tgacaggatt ttttcctgtc atgttttttt
     1441 attcgcccgc ataagatggc agtgaacgta caagcaaagg gaggaatagt atgcaaatcc
     1501 agacggataa cccatcatac gcaatcccat cgggtgatca tgtcgtcacg atgcggaacc
     1561 gaaaaagaat ggatcttaca tctgtaaaaa cgattgaccg gtttgaccaa gaagagttca
     1621 tcgtccggac atcgcaagga gtactgcaaa ttcgtgggga ggagctgcgt atcgtccatt
     1681 tggatgtaga caaagggctt cttactttgg aaggcacgat cggcacgatg caatatgacg
     1741 aagaagattc aggttcacgt aatttcctcc ataaattatt tggatgagtc tctccgtcca
     1801 gtttcttagc cttcttgcaa tgattgggac aggcatcggg gccggggttt tcatggattt
     1861 gatcggaacg ggcgtcgatg cgaccggcaa gaaatcgatc atccggaaat acgcggtccc
     1921 tttggaagtg atcggatgga ttttgatcgg gtgtgcatcg ttctacgttc ttttcatcgt
     1981 ccgggatggg gcgtggagga tgtatgaccc ggttgcccaa atttgcggca tgcttctgta
     2041 tgcttcgatt ttccaccggc ctttcaggtt tttcgggaga atcctgaaca tgttgatcat
     2101 caaaccgatc tggttcatca tcatgctcat agtgaagatc atcagctata tttacaggct
     2161 gattgtcaag attgtgggat tccttctttc tccgttcgtc tttctttatc ataaagtcta
     2221 taactctctc tttaaatctg ataagcaatg atgtataatt actagactta tcggatttcg
     2281 gaatgtaatg agggggagtg caggacatgg agcaaaggaa aaggaaatca tccacgaccg
     2341 ttgcatcgat tgaaactgaa tatgtccgct cgttgcgtaa aaaagaagtt tggagaaaca
     2401 agcaaaaaaa gcggctgcag aagaaactgt tcatctatgc agtgattgca ttcgtggtgc
     2461 tcggcgggtt gacgaatatg tatattcagc aacagcagac actgaaggag aaagaacagc
     2521 aaaaagtgga gacgctcgca aagttggaag aagtgaggga agagcaggaa cttcttaaaa
     2581 aacagcttgt gaaattagat gatgacgact atattgccaa gcttgcgcgg aaagaatatt
     2641 tcttatctga tgaaaatgaa atcatcttct cggttcctga aaataagaag aaaaaagacg
     2701 aaaaagatga cggaaaagag tagtcttatt gacactcttt ttttgttggc tataatgaaa
     2761 gtaaggatct tgcacttgca ggactcgcca tatggtaaag gcatctgaaa agaggccgtt
     2821 taaatcttaa ggaggagcat tttttttatg tcaattgaag taggcagcaa gttacagggg
     2881 aaagttacag ggatcacaaa cttcggtgcg ttcgtcgaac tgccgaacgg ttcaactggt
     2941 ctggtccata ttagtgaagt agcggacaat tatgttaagg atatcaatga ccacctaaaa
     3001 gtaggcgata tggttgaagt gaaagtgatg aatgtcggca ctgacggaaa aatcgggtta
     3061 tcgatcagaa aagcgaaacc ggaagctgaa cgtcctcaac gccctcaacg tcctcgtcac
     3121 agcggtggcc gtcctagcca taatgaccgt ccagagaatt ttgagcaaaa gatggcgaaa
     3181 ttcatgaaag acagtgaaga acgcctttca actttgaaaa gagcaacaga atctaaacgc
     3241 ggtggccgtg gcgcaagaag agggtaactt gctggctgtt tcaattagtg gaaatgaatc
     3301 tcttatgagc gatctgacat ctagttaaga tgttggatcg tttttttgtt tgcaaatacg
     3361 cataaatgcc gttaagcgcg cataaaattg cccagatacg cataaccgcg gtgaaagtcg
     3421 cataaattca tctaaatacg cataaccgtc ccgaaacacg cataaatgat aataatcacg
     3481 cataaaagct tccaaatgct cataaatgat tttattacaa ggatttcaac ctacagctgc
     3541 cgaataagag taatgaaaaa gggggttgta ttggaatgaa tgaaatcttg agcaatctta
     3601 gaaagcatgt agaatgggcg gagtcgctcc ataccttgtc tgaagaacag gcagacacac
     3661 cttatcaaga gggaaaatgg acgccgaagg agattctcat gcatatggcg gaatgggatc
     3721 ggttcacttt ggagcatcgt cttcaccgtt taaaagaatc agagaagctt gaggatgtgg
     3781 aatgggggcc attcaatgag caggctgcga aactttcaaa agaatattct tttgatgaag
     3841 tgattgcatt ggcgaagcag catcgtgaac aaattattga atggcttgaa ggcttggatg
     3901 aaacggaatg gacgaaaccg ttcaacatcg gcgagcatgt tttgacattg gaggattatt
     3961 tctcggattt tgtttggcat gattcgcatc ataaggaaca aatcgagtcg attcgataag
     4021 aagagaagga catttttgat ttatgcgtga ttggaagagt ttatgcgcgt ctgcgggagt
     4081 ttatgcgtat ttggaagaag ttatgcgtga gtttcctgtt ttatgcgtga ttgagcgagt
     4141 ttatgcgtga gtttccagtt ttatgcgcga ttcaggtgat ttatgcgtat ttcggtaata
     4201 aaaaaggcgt ccagtctgaa gactggacgc ctttttgagt tatagcggcg gagggggtcg
     4261 aacccacgac ctttcgggta tgaaccgaac gctctcgcca gctgagctac gccgccgtat
     4321 ggctccacag gtaggattcg aacctacgac cgatcggtta acagccgata gctctaccac
     4381 tgagctactg cggaatggtg ggcctaagtg gactcgaacc accgacctca cgcttatcag
     4441 gcgt
//
LOCUS       DASULI010000127         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1678747,
            whole genome shotgun sequence.
ACCESSION   DASULI010000127 DASULI010000000
VERSION     DASULI010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678747"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFX34_02775"
     CDS             complement(<1..761)
                     /locus_tag="VFX34_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563859.1"
                     /translation="MRNVIELKDVLFRRGNKDILTKIDWKIEKDEHWAILGLNGSGKT
                     SLLNIISAHQFPTEGEVHVLGNRFGETNLPELRKQIGYVSSSLERFAQMFQNDTVERV
                     IISGKFASFGLYEHPSPADWERADELLHDFRLSYLKGKRINLLSEGEKRRVLIARALM
                     NKPQMLIMDEPCSGLDILSREQFLHTLELVTKNGCHLVYVTHHVEELMEDITHVLLLK
                     EGRIVASGRKRDVMTDELLTETYKVPVKIQWDEGRP"
     gene            complement(825..1454)
                     /locus_tag="VFX34_02780"
     CDS             complement(825..1454)
                     /locus_tag="VFX34_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563860.1"
                     /translation="MQKTEAERVAPYERNFEIIGAEKDELIGKVNPAIFATVDRSNEA
                     AEQVESPVYTKEGVKVNLQPGRYMISGYPAGNIFIYDEKGDLVIREIVGHTGGVGSLT
                     VDIEGAYTVFADGGYDHVSFVPVPTALSTDLNAGIWEVGLDIEAGKYTFGSEYGLGYV
                     EIHEEGKEPVLYEVIGGNETGSRSHAELTEGQKIRITGISLVNFKSVKE"
     gene            complement(1582..>2287)
                     /locus_tag="VFX34_02785"
     CDS             complement(1582..>2287)
                     /locus_tag="VFX34_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563861.1"
                     /translation="LPLVPNPYVASNYLPVALGMISGLSFCLFVLIIGSSMLLNDQQH
                     RSVVGGFPISFMQKVGSKVSIHLLQIMLFLGLGVLVGGYYAARKTEWGNFITPVLIYQ
                     DMDFIAVSTMRYLTYMFIAFALIAIFLLLVFVLINVMTENLYATILTMLVILLLPDLM
                     SVVDVKFNWLYPLKFIDISSVLNGDAAMEFGNGKLDFKHSFRWLVGLNLIAGAVLYGR
                     NKLLYIRKREVAFKTN"
BASE COUNT          624 a          598 c          420 g          645 t
ORIGIN      
        1 ggtcttcctt catcccattg gattttcacg gggactttat acgtttccgt tagtaattca
       61 tcggtcatta catccctttt ccttccggaa gccacgatcc tcccttcttt taaaagtagc
      121 acatgtgtga tgtcctccat caattcctcg acgtggtgcg tcacatagac gaggtggcac
      181 ccgtttttcg tcaccaattc aagcgtatgt aaaaattgct ctctcgataa tatatcaaga
      241 cctgaacaag gttcatccat aatgagcatt tgcggtttat tcatgagcgc tcttgcaatt
      301 aaaactctcc gcttttctcc ttctgaaagc aagttaatcc gcttgccttt caaataggat
      361 aaccggaaat catgcaatag ctcatctgcc cgctcccagt cagctggaga tggatgctca
      421 tacaagccga aacttgcaaa tttcccacta atgatgactc gctcgacggt gtcattctga
      481 aacatttgag caaatcgctc caacgaactg ctaacataac cgatctgttt tcgcaattcc
      541 ggtaaattcg tttcgccaaa ccggttccca agaacgtgga cctctccttc ggttggaaat
      601 tgatgtgcag aaataatatt tagcagcgag gtcttgccgg atccattcaa ccctaaaatt
      661 gcccagtgct cgtccttttc aatcttccaa tcgatttttg taagaatgtc cttattgcct
      721 cttctgaaca aaacgtcctt cagttcaatc acgtttcgca tcctcaatca ctctctttca
      781 tcccattaat gaaaaacggc agatcatgtt gatccaccgt ttttttattc tttaaccgac
      841 ttaaaattaa ccaaggatat tccggtaatc ctgatctttt gaccctcagt caactccgca
      901 tggcttcttg aacctgtctc attgccgccg atcacttcat ataggacggg ctccttaccc
      961 tcttcatgga tttccacgta tccgagtccg tactcgctgc cgaaagtata cttacccgcc
     1021 tcaatatcca acccgacttc ccaaatgcct gcattcaaat cggtagagag tgctgtcggc
     1081 accggtacga aactgacgtg gtcataaccg ccatccgcaa aaacggtata cgctccttca
     1141 atatcaaccg tcaaactgcc cacgccccct gtatggccta caatttcgcg tatgacaaga
     1201 tcaccctttt cgtcgtaaat gaaaatatta ccggcaggat atccggaaat catatacctt
     1261 cccggttgaa gattaacctt aactccctct ttcgtataaa ctgggctttc aacttgttca
     1321 gctgcttcgt tgctccgatc aaccgtcgca aagattgctg gattcacctt tccaatcagt
     1381 tcatcctttt cagcgccgat aatttcaaaa ttcctttcat aaggagcgac ccgctcggcc
     1441 tccgtttttt gcaaaggatg gcatcctgac aacatgagca ggcttaaggc cagaaacaaa
     1501 gaaaagccgc ccatcctatt gaatcgtttc tgcatagcgt ttttcatttt cctccaccgt
     1561 tccttatatt atcctgcctt tttagtttgt tttgaatgcg acttcccttt ttcggatgta
     1621 aagcagttta ttccgtccgt agagaacagc tccagctatc agattgagcc caactaacca
     1681 acggaacgaa tgcttaaaat ccaattttcc attgccgaat tccatagccg catccccatt
     1741 cagcacggag ctaatatcga tgaatttcaa aggatataac caattgaatt ttacatctac
     1801 tacgctcatc agatcaggca gcagcagtat gacaagcatc gtcaaaattg ttgcataaag
     1861 attttcggtc atgacattga taaggacgaa tacgagcaaa agaaatatcg caattaacgc
     1921 aaaggcgatg aacatatacg tcagatacct catcgtcgac acggcgatga agtccatgtc
     1981 ttgatagatt agaacagggg tgatgaaatt tccccattcc gtttttcggg cggcataata
     2041 ccctcctaca agaacgccca atcctaggaa cagcataatt tggagcaaat ggatggagac
     2101 cttgctgcca actttttgca tgaatgagat cgggaaaccg ccgacaactg accgatgttg
     2161 ctggtcattc aataacatgc tgcttccgat gattaagacg aacagacaaa atgacaaccc
     2221 actgatcatg cctaaagcaa ccggcaaata attgctggcg acgtaaggat ttgggacgag
     2281 cggcaat
//
LOCUS       DASULI010000128         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1130085,
            whole genome shotgun sequence.
ACCESSION   DASULI010000128 DASULI010000000
VERSION     DASULI010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130085"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFX34_02790"
     CDS             complement(<1..205)
                     /locus_tag="VFX34_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX5563862.1"
                     /translation="MVPFTERVVQIIRDIPAGKVMTYGQVAAAAGNPRGARQVVRVLH
                     SMSAKYDLPWHRIVNAQGGISTPS"
     gene            289..846
                     /locus_tag="VFX34_02795"
     CDS             289..846
                     /locus_tag="VFX34_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563863.1"
                     /translation="MNKLFMKQSFIGYLIKIAGAVVIGWGVIQGIFYLSMMTQMGDPF
                     MNEWGHMHQMHYGGSLSGTALFAFLRIIATHFLYGLLIIGFGEVIDTLQKIYFRMDPE
                     AKRQWDEEQKEEAAFPQKHEVPFWVEQEVKGYYEKMQSNVQSIEKTSDPYVFKISVDD
                     RIEYIEVGNFEPRILSDEEAEKFKE"
     gene            complement(856..1515)
                     /locus_tag="VFX34_02800"
     CDS             complement(856..1515)
                     /locus_tag="VFX34_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5563864.1"
                     /translation="MKESTIRPPVAISFCILFAVAFAITATAIGRGMIRGFDQAIIDA
                     VQGLEAPRLTSFMQLFTAIGDTKVVMLLIVAVTAFLLLGLRNRRQAYFFFFALIGTGV
                     LNQTLKFIFKRARPEFHRLAEAAGYSFPSGHTMMSFSLYALFTYLLWGHIRSTAGRVI
                     LVIFAIFMFSMIAVSRIYLGVHYPSDILGGMFASAFWLTLAALLFAQYQKQRKHGLVQ
                     Q"
     gene            1602..2177
                     /locus_tag="VFX34_02805"
     CDS             1602..2177
                     /locus_tag="VFX34_02805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5563865.1"
                     /translation="MGISIMMICAYLAGNLLTATVVGKMMYRQDIRNSGSGNPGARNA
                     GRVLGKTAFVLTFIGDALKGAIIVLIAKWMGMDEWLQLVCILAVMAGHIFPVVHKFRG
                     GQGVSTFIGGMLAFNPLVVASFVVAFLIFYPFLKNFTAAGLSAMALSPLFLFGLTKEW
                     PQTVAAIIVVAFLLFAHRIDLYSMAKGYRKQ"
     gene            2208..2534
                     /gene="ytxJ"
                     /locus_tag="VFX34_02810"
     CDS             2208..2534
                     /gene="ytxJ"
                     /locus_tag="VFX34_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HEX5563866.1"
                     /translation="MKEIRSMEEWEHVRNQSQNEPVFLMKHSSTCPISSAGYRVFERY
                     ETDIPKYYLIVQRSRSLSNEIESELGIQHESPQLFLMKDGEAAWHASHYKISEYNIKS
                     AVEANG"
     gene            2827..3888
                     /locus_tag="VFX34_02815"
     CDS             2827..3888
                     /locus_tag="VFX34_02815"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5563867.1"
                     /translation="MSKEKTKQLHKNVAPYAKSDLKKSVIQLINTVPPIFLLWFLAYQ
                     SLSVSIWLTFGISVIAAGFVVRTFIIFHDCTHGSFFKNKKANDIVGTITGILTLFPYE
                     KWKREHAIHHATSSNLDKRGVGDIWVMTIEEYVEASNWEKFKYRCYRNPLVMFGFGPL
                     YLVLITGRINRKDARPKERNNTYLTNAVLIALYAFMVWLVGWQAFLIVQGSIMFVAAA
                     LGIWLFYIQHTFEDSYFEEESDWDYVKAAVEGSSYYKLPKVLQWATGNIGFHHVHHLA
                     PRVPNYNLELAHESTPPLQMATTITIKTSLESLRYKLYDPDNKRFITFKEVKNAVLRT
                     KRSIDLNELKPKRTSFESK"
     gene            3962..>4154
                     /locus_tag="VFX34_02820"
     CDS             3962..>4154
                     /locus_tag="VFX34_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5563868.1"
                     /translation="MQKWYSIFPKNPWLSIYAWAVFCLLPFFFIFRSWSAVGISVGTG
                     LLVLYFISYFFSFKSKSGLV"
BASE COUNT         1148 a          931 c         1037 g         1038 t
ORIGIN      
        1 cagatggagt cgagatgccg ccttgcgcat tgacgatccg gtgccaaggc aagtcgtatt
       61 tcgcgctcat cgaatgaagg accctgacga cttgcctcgc gccgcgcgga ttgcccgctg
      121 ctgcagcaac ttgtccatac gtcatgacct tgccggcggg gatatcccgg atgatctgca
      181 caacccgttc tgtaaatgga accatgtcga acctcctcgt gatgaaacta tttagctgac
      241 catatcgtac agtatagaaa tggaaaataa aataggaggg tggtcacatt gaacaagttg
      301 tttatgaagc agagtttcat cggctatctc attaaaatcg ccggagctgt cgtgattgga
      361 tggggcgtca tccaaggaat cttctatttg tcgatgatga cacaaatggg cgatcccttc
      421 atgaatgaat ggggacatat gcatcaaatg cattatggag gcagcctttc aggcactgca
      481 ctgttcgctt ttcttagaat tattgcaacg cacttccttt atggcttgct catcatcggt
      541 ttcggtgaag tgattgatac gttgcaaaag atttatttcc gcatggatcc tgaagcaaag
      601 cggcaatggg atgaagagca gaaggaggag gcggctttcc ctcagaaaca tgaggtgccg
      661 ttttgggtgg agcaggaagt gaagggctat tacgaaaaaa tgcaatcgaa cgtccaatcc
      721 atcgaaaaga cgagcgatcc ctatgtgttc aaaatttcgg tcgatgatcg catcgaatat
      781 attgaagtgg ggaacttcga accgaggatt ttatccgatg aagaggcgga aaaatttaaa
      841 gaataaacgg ccgcgtcact gttggacgag gccgtgtttc ctctgttttt gatattgggc
      901 gaacagcaaa gccgcaagcg tcagccagaa ggcgcttgca aacatgccac cgaggatgtc
      961 actcggataa tggacgccaa ggtagatgcg gctcacggca atcatactga acatgaaaat
     1021 tgcaaagatc accaggatga ccctgcccgc agtcgaacgg atatggcccc atagcaaata
     1081 cgtgaataat gcataaagac tgaagctcat catcgtatgg ccgcttggga agctatagcc
     1141 tgcagcttca gcaagccgat ggaattcagg ccgcgcccgt ttgaaaatga atttcagcgt
     1201 ttgattcaac acgcctgtcc cgatcagcgc aaagaaaaag aaataggctt gtcttctgtt
     1261 gcgcaggccc aataacaaaa aggccgtcac ggcaacaatt agaagcatga caactttcgt
     1321 gtcgccgatg gcagtgaaaa gttgcatgaa cgacgtcaac cgcggtgctt ccaagccttg
     1381 tacggcatcg atgattgcct ggtcgaagcc cctgatcata cctcgtccaa tcgcagtcgc
     1441 ggtgatggca aaggcaactg cgaataaaat acagaaacta atggccacgg gagggcgaat
     1501 ggttgattcc ttcaactaga tcatcctttc tcttgcgaca tattgtacag gaaaagacgg
     1561 gattccgcat attgtaaaga gggaatagga agtgaagatg catgggaatt tccataatga
     1621 tgatttgcgc atatttggcc ggcaacttgc tgacggcaac tgtagtcgga aaaatgatgt
     1681 atcgacagga tatccgcaat tcgggcagcg gcaatcccgg cgcacgcaat gcggggaggg
     1741 tgctcggcaa gaccgcgttt gtcttgacat tcattggaga tgccttaaaa ggcgcgatca
     1801 tcgtcttgat cgccaaatgg atggggatgg atgaatggct gcagttggtg tgtattcttg
     1861 ccgtcatggc aggccatatt tttccggtcg tccataagtt ccgtggcgga cagggggtgt
     1921 cgacattcat cggcgggatg cttgcattca acccgctcgt cgttgctagt tttgtagtcg
     1981 cattcctcat cttctaccca ttcctgaaaa actttacagc tgcgggttta agcgccatgg
     2041 cattgtcgcc gcttttcctg ttcggtttga cgaaggaatg gccgcaaacg gttgccgcta
     2101 taatagtagt ggcttttcta ttattcgccc atcgaataga tttatatagt atggcaaaag
     2161 ggtatagaaa gcagtaaggc gatcatcggg aaaggagagt gcatctaatg aaagagattc
     2221 gatccatgga agaatgggag catgtgcgca atcaatctca aaatgaaccc gtcttcttaa
     2281 tgaaacatag ttcgacatgt ccgatcagtt ccgcgggata ccgggtgttt gagagatatg
     2341 aaacggatat tccgaaatac tatttgattg tccaacgaag ccgctcactg tctaatgaaa
     2401 tcgaaagcga attgggcata cagcatgaaa gcccgcagct gttcttaatg aaggacggcg
     2461 aagcggcatg gcatgcaagc cactacaaaa ttagcgaata caatatcaaa tcagccgtcg
     2521 aagcgaacgg gtaagaaatg cagacatccc ttaacggggt gtctgttttt tagtgatttg
     2581 tggcgagaca aaagtcccga gatgagcata actccgtgag attgcgccat aactcgttcg
     2641 aactcgagca taatacaccc aaactcgagt ataacccatc aaaactcgag caaaacgcca
     2701 attcttgtga atctcttcac aataaatgac acttatcata tgcaaagtta tgacgttcat
     2761 ctattctgca taatgggtat gcagtttagt atggaaataa cgaaaattct cgaggaggcg
     2821 ttcacattga gcaaggaaaa aacgaagcaa ttgcacaaaa atgttgcacc ctatgccaaa
     2881 tcggatttga aaaagagtgt catccaactg atcaatacgg ttccgcccat attccttcta
     2941 tggtttttgg cttatcagag tttatcggtt tccatttggt tgacgtttgg catctctgtc
     3001 atcgcggcgg gctttgtcgt gcggacattc atcattttcc acgactgcac gcacggttca
     3061 tttttcaaaa ataaaaaagc gaatgatatc gtcgggacga ttacgggaat cctcactctg
     3121 ttcccatatg aaaagtggaa acgcgagcat gcgatccacc atgcgacaag ttccaaccta
     3181 gataagcggg gcgtcggcga tatttgggtt atgacaatcg aagaatatgt cgaagcttcg
     3241 aattgggaga aattcaagta tcgctgctac cggaacccac tcgtcatgtt cgggttcgga
     3301 ccattgtatt tggtgctcat tacaggcaga atcaaccgga aagacgcacg tcctaaagaa
     3361 cgcaataaca cctatttgac gaatgccgtc ctcatcgcgc tctacgcgtt catggtatgg
     3421 ctcgttggct ggcaggcatt cctcatcgtg caaggctcga tcatgtttgt cgcggcagcg
     3481 ctcggcatct ggcttttcta tattcagcac acattcgagg attcctattt cgaagaggag
     3541 tccgattggg attatgtgaa ggcggcagtg gaaggcagct cgtattacaa attgccgaaa
     3601 gtccttcagt gggcgacagg caacatcggc ttccaccacg tccaccattt ggcgccgcgc
     3661 gttccgaact acaacttgga actggcgcac gaatcgacac cgccacttca aatggcaacg
     3721 acaatcacga ttaaaacaag cttggaatca ctccgctata aactatacga tccggataac
     3781 aagcgtttca ttacgttcaa agaagtgaaa aacgcggtct tgcggaccaa gagaagcatt
     3841 gatttgaatg aattgaagcc gaaacgaaca agctttgaaa gtaaataaac aaatgcagcc
     3901 gcctttcgat gaggcggctg cgtgtttttg gtacaatgga agaaacaggg cggtgaattg
     3961 gatgcagaaa tggtacagta ttttcccgaa aaatccgtgg ctcagtattt atgcatgggc
     4021 tgttttttgc ctgctgccgt tctttttcat cttccggtca tggtcagcgg tgggaatttc
     4081 agtcggcacc ggactattag tcctttattt catctcctac ttcttttcat tcaaatcaaa
     4141 aagcggtctc gtct
//
LOCUS       DASULI010000129         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1944612,
            whole genome shotgun sequence.
ACCESSION   DASULI010000129 DASULI010000000
VERSION     DASULI010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944612"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..943
                     /locus_tag="VFX34_02825"
     CDS             257..943
                     /locus_tag="VFX34_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563869.1"
                     /translation="MFFDYRFWHFLTRPYDLATQIQTGTMRHFNRRLLILFLAGIALF
                     ALREIWGMGTEALTSLMTTMTTTDYIIARYASLAGTVIWSILYIAFHVFGVAWILSML
                     TKIPYRQLVPLQVLMTGLLLLEKALLFIVFAIKGATVNLSFLSFGPLAVTVMENWYLI
                     LFMNQLTITSALIITLQYRYIRGFLADGRWREHLWILIGLHVAMALITAAVAYIPFDS
                     ILNSLFGGGF"
     gene            944..2218
                     /locus_tag="VFX34_02830"
     CDS             944..2218
                     /locus_tag="VFX34_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5563870.1"
                     /translation="MNKRLSITVAIIVSLFIAVNVHLLFSKKSMVPESVYVHQYERMT
                     AGHHEEELLKEGLITPEDIYTVYVGDDETIDSWLVKEGDPVQVGDEIALLQTERAKSQ
                     LATWEAEEEGLLEQRDSIESTIASLESDRGSAGNNSSHVNTTETPGDSDVELTVDVQV
                     DVHQDGAFAQAIAEAERELAEIDRKLAVVQTQLAQDAARPALISPVNGTVSKVFKHGF
                     TLAADVYSDVKLVTTYVQLEEWQKIEAGDSVRIAENSLETAMEGTVHSVSEVPATDSR
                     WREAYDKLDPKERNNPLDVYEITIKPDEELSSLPFGANVNAAIIVNEADAVSVKIHWL
                     EAYMKDSALAWKLDKNGRAAKTQVTVPFIDDSRAVITEGLTTGDVAIYNRKFDEFNNV
                     SGVFLPIPLELPSKKDWRTFGWKNYLKYGFVE"
     gene            2397..>2768
                     /locus_tag="VFX34_02835"
     CDS             2397..>2768
                     /locus_tag="VFX34_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563871.1"
                     /translation="MKKGIVLAIIFISFILGVKEVTAKEDAQRYIGLHDKMLPIEYIT
                     EIDGDTKVLFKDITKHLYIDVTEEKGALKLNKHGKEIILPMPAKSDAAQTGSPLFIDE
                     SGELLISLSYLADQFEFKLQYF"
BASE COUNT          766 a          683 c          668 g          651 t
ORIGIN      
        1 gaaaacagtg gaaccggtca atccgaacaa gaaatcctgc acagtgaatc gatgtttatc
       61 gtcttgatcc atcaaaatcc gctcctttgt cgatttcccg ggaaatgagg cgtttcaggg
      121 aatgaacttt tctaactaga ctggccaaat tcaccaattt ttatacaaaa ttgcctacta
      181 aaaattgaat aatccacgag atttgataca gttaagtata tagcaactaa aatatctgca
      241 gaaaggattg tcgcgcttgt ttttcgatta ccgattttgg cactttttga cgaggcccta
      301 cgaccttgcc acccagatac aaaccggaac gatgcggcac ttcaatcgaa ggcttctcat
      361 cctcttcctg gcgggcatcg ccctcttcgc gctccgggaa atttggggca tgggcacgga
      421 agcacttacg tcattgatga cgacgatgac gacgacagac tacataattg caagatacgc
      481 ctcacttgcg ggtacggtga tatggtcaat cctatacata gcattccatg ttttcggcgt
      541 cgcatggatc ctgtcgatgc tgacaaaaat cccataccgg cagcttgttc cgctgcaagt
      601 gctcatgacg ggcttgctgc tcctggaaaa ggcgttgctc ttcatcgtct tcgccatcaa
      661 aggcgcgacg gtcaacctat cgttcctatc cttcgggccg ctcgccgtca cggtcatgga
      721 aaattggtat ttgatccttt tcatgaacca attgacgatt acgagcgcac tgatcatcac
      781 attgcaatac cggtacatcc gggggttcct tgcggacggc agatggcggg agcatctgtg
      841 gatattgatc ggcctgcatg tcgcaatggc gctcattaca gcagccgtcg cctacattcc
      901 attcgactcc atcctcaatt cgcttttcgg aggtggcttc tagatgaata aaagattaag
      961 catcactgtc gccattatcg tcagcctgtt tattgccgtg aacgtacacc tgcttttctc
     1021 gaaaaaaagc atggtccctg aatcagtgta tgtccatcag tacgagcgca tgactgccgg
     1081 acatcatgag gaggaattac tgaaagaagg actcatcacg cctgaagaca tctacaccgt
     1141 ctacgtcggc gacgatgaga ctatcgattc atggctcgtc aaggaaggcg atccggttca
     1201 ggtcggggat gaaatcgcgc tcctgcagac tgaacgcgca aagagccagc tcgcgacctg
     1261 ggaagcggaa gaggaggggc tccttgaaca acgggattca atcgaatcaa cgattgcaag
     1321 cctcgaatca gatcggggca gcgccggcaa caattcgtcc catgtgaata cgaccgaaac
     1381 acctggtgat tccgacgtgg aattgacggt cgatgtccaa gtggatgtcc atcaagacgg
     1441 cgcattcgcc caagcaatcg ccgaagcgga gcgtgaactg gcggaaattg acaggaaact
     1501 cgccgtcgtt caaacacagc ttgcccaaga cgccgcccgt cccgcactca tcagtccggt
     1561 gaatggaacg gtttcgaaag tgttcaaaca tggctttacc cttgccgccg atgtctacag
     1621 cgatgtgaaa ctagttacga cttacgtaca attggaagaa tggcagaaaa tcgaagccgg
     1681 tgattccgtc cgcattgcgg agaattcttt ggagacggcg atggaaggca cagtccactc
     1741 cgtatccgaa gtgcccgcga cggacagcag atggcgcgaa gcatacgaca agctggaccc
     1801 gaaagaacgg aacaacccgc tcgatgtgta tgaaatcacg atcaagcctg acgaagaatt
     1861 gtccagcctt cctttcgggg caaacgtcaa tgcagcgatc atcgtgaatg aagcggatgc
     1921 cgtctctgtg aaaatccact ggcttgaagc gtatatgaaa gatagcgccc tcgcatggaa
     1981 gttggataag aatggccgtg ctgccaagac gcaagtcacg gttccattca ttgatgacag
     2041 ccgcgccgtc atcacggaag gcctaacaac cggcgacgtc gccatctaca atcggaaatt
     2101 cgacgaattc aataatgtat caggtgtttt cctgcccatc ccgctggagc ttccgtccaa
     2161 aaaagactgg agaacattcg gctggaagaa ctatttgaaa tacggatttg ttgaataaac
     2221 gccaatgaaa aaaccgccca cgggcggttt ttttacgtca acatatatga tttccaatta
     2281 ttcttttcac tcagaaactt agatcgagcg ggggggctgc ccaattctat tctactttcc
     2341 tttctccatt aatagaatga aacaatgaat tcataataga aatggagaag gtaaagttga
     2401 aaaagggaat cgtactagca atcatattca tctccttcat acttggcgtg aaagaagtga
     2461 cggcgaaaga ggatgcgcag cggtatatcg gccttcacga caagatgctg ccgatcgagt
     2521 acatcaccga gatcgacggg gatacaaaag tgctgttcaa agacattaca aaacatttgt
     2581 acattgacgt caccgaggag aaaggggcac taaaattaaa taaacacggc aaggaaatta
     2641 tacttcccat gcctgccaaa tcggacgcag cacaaacagg aagtccgctt tttattgacg
     2701 agtccggcga attgctgatt agtctatcgt atttggctga ccaatttgaa ttcaagcttc
     2761 aatacttt
//
LOCUS       DASULI010000130         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1327935,
            whole genome shotgun sequence.
ACCESSION   DASULI010000130 DASULI010000000
VERSION     DASULI010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327935"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..465)
                     /gene="gcvH"
                     /locus_tag="VFX34_02840"
     CDS             complement(82..465)
                     /gene="gcvH"
                     /locus_tag="VFX34_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003327010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5563872.1"
                     /translation="MNTPKEFRYSEEHEWVKDEGGKYRIGITHFAQSELGDIVFVELP
                     QVGDEVKADEPFGSVESVKTVSELYAPISGKVVEVNEELEDSPELVNESPFEGAWMIV
                     VEASDESELDALMSAEDYDKMTVGE"
     gene            complement(548..907)
                     /locus_tag="VFX34_02845"
     CDS             complement(548..907)
                     /locus_tag="VFX34_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007200656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEX5563873.1"
                     /translation="MSLTYYGYPKCGTCRKAKKWLENAGLEFEDVNIVENPPSKEELQ
                     QMIENSGLEIKKFFNVSGMKYRELNLKDKLAEMTDDEKVELLATDGMLMKRPLVTDGA
                     KVTVGFKEETYKEVWGA"
     gene            complement(982..2769)
                     /locus_tag="VFX34_02850"
     CDS             complement(982..2769)
                     /locus_tag="VFX34_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5563874.1"
                     /translation="MTESTTKDFIRGGAFLIEDIDASRVFTPEDFSDEQKMIAKTTED
                     YVKNEVLPVVENLENHEFEHSVRLLKSAGDLGLLGADVPEEYDGLGLDKISSALIAEK
                     MSVAGGFSITHGAHVGIGTLPIVLFGNEEQKKKYLPNSVSGDKISAYALTEPGSGSDA
                     LGAKTTAKLNEAGTHYVLNGEKQWITNAGFADVFVVYAKVDGDKFTAFIVEREFPGVS
                     VGAEEKKMGIKSSSTRTLILEDAQVPVENLLGEIGRGHIIAFNILNIGRYKLGVGTVG
                     GSKRAFELAVTYANQRQQFKTPISSFNLTKEKLSTMASKIYATESLIYRTVGYFEERN
                     SQMTPEQQKDGKAVAASIAEYAIECSINKVVGSEVLDYVVDEAVQLHGGYGFMQEYEV
                     ERAYRDSRINRIFEGTNEINRMIVPGTFLKKAMKGELPLLEQAQSLQEELLMMMPEEI
                     GDEVLAQEKVLVKNAKKIGLLAAGMAAQRFGTQLDKEQEVLVNIADIANNIFAMESAL
                     LRTEKAIAKNGEEKEQQKILYTQIFCQEAFEAIEKDAKETLLASVEGDNQRMMLSALR
                     KLTRSNPYNIIAKKREASVKLIDAEKYVV"
     gene            complement(3167..4342)
                     /locus_tag="VFX34_02855"
     CDS             complement(3167..4342)
                     /locus_tag="VFX34_02855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019156207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5563875.1"
                     /translation="MREAVIVVGARTPVGKAGRGSLATVRPDDLGALTIKETLKRANG
                     YDGPIDDLIIGCAMPEAEQGMNVARNIGALAGLPDTTPAITVNRFCSSGLQSIAYAAE
                     RIMLGHSEAIIAGGVESMSMVPMMGNTIRPNFKLAETAPQYYMGMGHTAEQVAMKYGV
                     SREDQDAFAVRSHEKAAAAIAAGKFVDEIVPVEVVKRFVDENGKYQEKTVLFEMDEGV
                     RTGTSMEVLAKLRPAFSVKGSVTAGNASQTSDGAASVLVMDREIAEGKGLKPIAKFRS
                     FAVGGVPPEVMGIGPIEAVPKALKLAGLSIEDIDLWELNEAFASQSLQVVRHLGIDMD
                     KVNVNGGAIALGHPLGATGSVLTLKLMNELKRQGKQFGVVTMCIGGGMGAAGVFEML"
     gene            complement(4373..>4803)
                     /locus_tag="VFX34_02860"
     CDS             complement(4373..>4803)
                     /locus_tag="VFX34_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX5563876.1"
                     /translation="MAKVSTSAEEAKENNFLDFADGISVNPDHLIYDAKQAALSLYEA
                     GYTPPKKEKIPVTGDSGYATMLLGAEGMHLSGYISDHDLKIAKKLAFVLSGRKVPYGT
                     LVDEQYMLDLEREAFLSLIQEPKSQQRMQHMLLKGKPLRN"
BASE COUNT         1078 a         1293 c         1029 g         1403 t
ORIGIN      
        1 gacattccat tcgcacgatt cctcctcttt aaaccgataa aaaaagcgcc ggaatcagat
       61 atccaggcgc cactgcaacg attactcgcc gacagtcatt ttgtcgtagt cttcagctga
      121 catgagtgca tcgagttcgg attcgtccga tgcttccaca acgatcatcc aagcaccttc
      181 aaatggagat tcattcacca attcagggct gtcttccaat tcttcgttca cttcgacgac
      241 tttaccgctg atcggagcat atagttcaga tacagttttt actgattcaa cacttccgaa
      301 tggctcatct gctttcactt catctccaac ttgcggcaat tcaacgaaaa cgatatcgcc
      361 aagttcggat tgtgcgaaat gtgtaatacc gatacgatac tttccgcctt catcttttac
      421 ccactcatgt tcttctgaat aacgaaattc tttaggtgtg ttcatcccgt acccctccat
      481 ttaagtatgc atttattgat ttccattcaa ttttgacata tccggaagct aaagacaaga
      541 aagtatatta ggctccccat acttccttat atgtttcttc cttgaagccg acagtcactt
      601 tcgcgccatc tgtcacaagc ggtctcttca tgagcatgcc gtccgtcgcc agcagctcga
      661 ccttctcgtc atccgtcatc tcggccagct tatccttcaa attcaattca cggtatttca
      721 ttccgctaac attgaagaac ttcttaatct ccaggccgga gttctcgatc atttgttgaa
      781 gctcctcttt tgacggcggg ttttcaacga tattgacgtc ttcaaattcc aaccctgcat
      841 tttccaacca ctttttcgct ttccggcatg ttccgcattt tggatatccg tagtatgtta
      901 aagacatttc gcaacacctc cattttcccc aagtataaca gagaagccgc ccggtataaa
      961 aataacccgg gcggtgtgca atcatactac gtatttttca gcgtcgatca atttgacgga
     1021 tgcttcgcgt tttttcgcga tgatgttgta cggattcgaa cgcgtcaatt tgcgcaatgc
     1081 agacagcatc atgcgctggt tatcgccttc gacggatgca agaagtgttt ctttcgcatc
     1141 cttttcgatt gcttcgaacg cttcctggca gaagatttga gtgtaaagga tcttctgttg
     1201 ttctttctcc tcgccatttt tagctattgc tttttccgta cgtaatagcg cggactccat
     1261 tgcaaagatg ttatttgcga tgtcagcaat attcacgagg acttcttgtt ctttatcaag
     1321 ttgagtgcca aatcgttgtg ccgccattcc agctgcgagc aaaccgattt tcttcgcgtt
     1381 tttaacgagc actttctctt gggcaagcac ctcgtcgccg atttcttccg gcatcatcat
     1441 gagcagctct tcctgcaagc tttgtgcctg ctcaagcaaa ggcaattcgc ctttcatcgc
     1501 tttcttcaag aacgttcctg gaacgatcat gcggttgatt tcatttgttc cttcaaagat
     1561 acggttaatg cgggaatcgc ggtaagcgcg ctccacttca tactcttgca tgaaaccgta
     1621 tccgccatgc aattggacag cttcgtcaac gacgtagtca agcacttcgg agccgacaac
     1681 tttgttgatg gagcattcga ttgcatattc tgcaatggaa gctgcaaccg cttttccgtc
     1741 cttctgctgt tccggagtca tttggctgtt gcgctcttcg aaatacccta ctgtacggta
     1801 gatcaagctt tctgtcgcat agatctttga agccatcgtc gaaagctttt cttttgtaag
     1861 gttgaatgaa gagatcggtg ttttgaactg ttggcgctga ttcgcatacg taaccgccaa
     1921 ttcaaatgca cgtttggaac ctccaactgt acctacacct aatttataac ggccgatgtt
     1981 caagatgttg aatgcgatga tatgccctct gccgatttca cctaacaggt tttcaacagg
     2041 aacttgtgcg tcttcaagga tcaacgtacg cgtggaagaa gacttgatgc ccatcttctt
     2101 ctcttccgct ccgacagaaa cgcctgggaa ttccctttca acgatgaatg ctgtgaattt
     2161 atcgccgtcc actttcgcat agacgacgaa tacgtctgca aaccctgcgt ttgtgatcca
     2221 ttgtttttca ccgttcaaca cataatgcgt gccggcttcg ttcagcttcg ctgttgtctt
     2281 tgcaccaagt gcgtccgatc cggagcctgg ttcagtcaat gcgtaggcgg aaattttatc
     2341 gccggatacg gagttcggca aatacttttt cttctgttct tcattcccga aaaggacgat
     2401 cggcaacgtc ccgattccga cgtgcgctcc gtgtgtgatg gagaagccgc ctgcgacgga
     2461 catcttttct gcaatcaatg cggatgagat tttatcaagg ccgaggccgt cgtattcttc
     2521 aggaacgtcg gcaccgagaa ggccaaggtc cccagcagat ttcagtagtc tgacagagtg
     2581 ttcgaattca tggttctcca ggttttccac gactggaaga acttcgtttt tcacgtagtc
     2641 ctctgttgtt tttgcgatca ttttttgttc gtcggaaaag tcttctggtg taaatacgcg
     2701 tgatgcgtcg atgtcctcga taaggaatgc tcctccgcgg atgaaatctt ttgttgtcga
     2761 ttcagtcatt ataattcctc ccataaatta attttaataa aaagtcctat ataacggatt
     2821 ttcacttcgg gcggacgctt tccgccggca tggcttcagc caatcgaaca gcgaagggtt
     2881 cgatttgccg tatttctgct ccttttgcag aaattaaggc atcatcatcc tgctcgcaac
     2941 actacgcttt tgctcgcaaa agccgttctt cgtgacggct ttggcaggag tcgccgccct
     3001 gcgcttcaat ccttggtttc tatttattga actcataatt cacttcaaat acgcttaaat
     3061 cactgcaaac gcgcataaac ggtcccaatt acgcataatc cttcagaaag attcataact
     3121 tcacgcaaat acgcgtaaaa gcctctaacc gctcataaag caattcttat agcatttcaa
     3181 atacgccggc tgcgcccatt cctccgccta tgcacatggt gacgacaccg aattgcttgc
     3241 cttggcgttt caactcattc atcagcttca atgtcaggac agagcctgtt gcgcctagcg
     3301 ggtggcctaa cgcgatggct ccgccgttga cgttgacttt atccatatcg atgcctaagt
     3361 ggcggacgac ttgcaaggac tgggaagcga aagcttcgtt cagctcccat aggtcgatgt
     3421 cttcgatgga aagtccggca agcttcaatg cttttggaac ggcttcgatc ggtccgatcc
     3481 ccatcacttc aggcggaacg ccccctacgg cgaatgaacg gaattttgcg atcggcttca
     3541 agccctttcc ttccgcgatt tcacggtcca tgacgaggac ggatgccgcg ccatcggaag
     3601 tttgcgacgc attcccggca gtgacggaac ctttcaccga gaaagccggg cgtaatttcg
     3661 ccaacacttc catcgatgtg cccgtgcgga cgccttcgtc catttcaaac aggaccgtct
     3721 tttcctgata tttgccgttt tcatccacaa aacgcttcac gacttcgaca ggtacgattt
     3781 catctacgaa tttgccggct gcgattgctg ctgccgcttt ttcatgtgaa cgaacggcaa
     3841 atgcgtcctg gtcttcacgg ctgacgccgt atttcattgc cacttgttcg gctgtatggc
     3901 ccattcccat atagtactga ggtgctgttt cagccaattt gaagttcggt ctgatcgtat
     3961 tccccatcat cggcaccatg ctcatcgact cgacgccgcc cgcaatgatc gcttcggaat
     4021 gtccgagcat gatccgctct gctgcatagg caatcgattg gagtcccgat gaacagaacc
     4081 ggttgaccgt aatcgcaggc gtcgtatcag gcaaacctgc cagcgcgccg atattgcggg
     4141 cgacgttcat cccttgctcc gcttccggca tcgcacaccc aatgatcaag tcatctatcg
     4201 gtccatcgta tccatttgca cgtttcaaag tttctttaat tgtcaaagcc cctagatcat
     4261 ccgggcgaac agttgccaaa gagccacggc ccgcttttcc gaccggagtc cttgcaccta
     4321 ctacaattac tgcttcacgc atttgacttc ctccttttgc tttatgtgca gatcagttgc
     4381 gcagcggctt ccctttcaga agcatatgct gcatccgctg ttgggatttc ggctcttgaa
     4441 tcaggctcag gaacgcctcc cgctcaaggt ccaacatgta ttgttcgtcg acaagagttc
     4501 cataaggcac ttttctgccc gataaaacaa atgcgagttt cttcgctatt tttaagtcat
     4561 ggtcgctaat atagcctgac aaatgcatgc cttccgctcc gaggagcatc gttgcatacc
     4621 cggaatctcc cgtgaccggg atcttctctt ttttcggcgg tgtatagccg gcttcataca
     4681 aggacagcgc agcctgcttc gcatcataga tcaaatggtc cggattgacg ctgatgccgt
     4741 ccgcgaagtc gaggaagtta ttctctttcg cctcttcagc ggatgtcgac actttcgcca
     4801 tcg
//
LOCUS       DASULI010000131         5889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_234540, whole
            genome shotgun sequence.
ACCESSION   DASULI010000131 DASULI010000000
VERSION     DASULI010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234540"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFX34_02865"
     CDS             <1..618
                     /locus_tag="VFX34_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5563877.1"
                     /translation="IVETLSHEGKTVVSMKDSEGILAVIALKDTLRPEAVQAINDLKQ
                     LGIRSIMLTGDNEKTANAIAKEAGLDRFAAECLPEMKVEELKRLLKEYGEVGMVGDGI
                     NDAPALATATSGIAMGEGTDVALETADVVLMQNDLTRISYAVKVSKKMQRIVKQNVFF
                     SIAVIAVLIISNFMQVVDLPLGVIGHEGSTILVILNGLRMLNKVD"
     gene            complement(639..1625)
                     /locus_tag="VFX34_02870"
     CDS             complement(639..1625)
                     /locus_tag="VFX34_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5563878.1"
                     /translation="MKHKDPLPPKKEKRQTEEEKSGFLANNKIMKFFDDVKDGDLEKF
                     DVTTSNGFWKELLVRIKKVDITGLGSQLAFFFLLSLFPLLIFLITLLPFLNLDESQVF
                     LFIRDYAPDSVYTLIHDTIEEILKNRNGGLLSIGALATIWSASKGMNALTKALNRSYY
                     VEETRSFLISRAMSVVFTIALIGVLIIALILPVFGQQIGMLAFSYFGMEEGFLRLWGS
                     MRWVIPPILIFLVFSALYWAVPNLKIRLKSAMPGALFATAGWILTSLAFSFYVGSFAN
                     YSKTYGSIGGIIVLMMWLYFSAIILMLGGQLNAVMTERKQAKLAKEKSNAVS"
     gene            complement(1649..1996)
                     /locus_tag="VFX34_02875"
     CDS             complement(1649..1996)
                     /locus_tag="VFX34_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX5563879.1"
                     /translation="MLGAILGGAISMFDRSTRYQVKRKSSAMIEQVGYYSKNPDVLKQ
                     KLQQQKDKYLSIYEQFSGDAAYIKNQVEELKTLTPQVKELVVGTKEAFVDSKEEYKEI
                     VNESKEGMETKKM"
     gene            2286..3674
                     /locus_tag="VFX34_02880"
     CDS             2286..3674
                     /locus_tag="VFX34_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEX5563880.1"
                     /translation="MDAITSFLDKASGFIWGPPLLVLLVGTGIFLTVRLGFIQVRLLP
                     YSLKQVFSRKHSKKAEGDISQFQALMTAMAATVGVGNIVGVATAVYMGGPGAIFWMWL
                     AGFFGMATKYGEAILAVKYRVKDSKGEMAGGPMYYLEHGLKQKWLGVAFAIFGALAAF
                     GIGNGTQSKAVADVMKSTFNVPHLVTGIALLIFGALVILGGIKSIGRVTAFFVPIMAL
                     FYFIAGAIVMIMNIELVPAAFGLIFSDAFTGQAAAGGAIGAVIRFGVARGLFSNEAGL
                     GSAPIAAAAAKTDMPGRQALISMTQVLFDTIIICSITGVTIVMSGQWKDKTIDAGALT
                     AQAFGEFLGGAGPIIVAIGIVFFATSTILGWGYYGEKCFQYLFPSNGAVLGYRIAFVA
                     FIIVGSLVSLDIVWLLADVLNGLMAIPNLIGLLGLSGVIVMETKRFRAKIAEEKEAEK
                     MGKTAPVDSTVD"
     gene            3718..3939
                     /locus_tag="VFX34_02885"
     CDS             3718..3939
                     /locus_tag="VFX34_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 domain-containing protein"
                     /protein_id="HEX5563881.1"
                     /translation="MDLSQPTPENVTFMIEKIKEKLRMVNVDAMKAENFNTSQYEDLV
                     YIYEMVMKRDTITPNEMQAIAAELGSLRK"
     gene            complement(3996..4757)
                     /gene="motB"
                     /locus_tag="VFX34_02890"
     CDS             complement(3996..4757)
                     /gene="motB"
                     /locus_tag="VFX34_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX5563882.1"
                     /translation="MSRKRKRRNKEEGHINESWLLPYADLLTLLLAMFIVLFASSTLD
                     EAKFSQISSVFNQIFDGGTGVMDNSAPTPVPIPKDSAGEENENSSYLEDQQSLEEIRD
                     KLDEYIAVHELENQFQTKLTEEGLLITIRDSILFESGKATIKPQYVTLSKDIANLLVF
                     DKPRQIVITGHTDNVPINSAEFPSNWELSMMRAINFLKTVVSNDQLDPQLFSVKGYGE
                     NKPVASNDTAEGRSKNRRVEVLIQPLVLKDGTSVE"
     gene            complement(4741..5556)
                     /gene="motA"
                     /locus_tag="VFX34_02895"
     CDS             complement(4741..5556)
                     /gene="motA"
                     /locus_tag="VFX34_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX5563883.1"
                     /translation="MDLSTLFGLILGMVALFVGMAMKGVTPDNLLNIAAILIIIVGTM
                     AAVVIAFPMKEIKRVPKLFGIIFREQNLPADSDLIKMFSGWADVARREGLLSLEAKAD
                     EITDPFLRNGLGLAIDGQNADYIRDVLTEEVDAMEDRHSAGAQIFSQAGTYAPTLGVL
                     GAVVGLIAALKNMNDIDALGHAISAAFVATLLGIFTGYVLWHPFANKLQRKSKEEVRQ
                     KMMMIEGILSVLEGEAPRVIEQKLASYLSMEERRKLAAKNTSDKGAGQVVEET"
     gene            5774..>5889
                     /locus_tag="VFX34_02900"
     CDS             5774..>5889
                     /locus_tag="VFX34_02900"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5563884.1"
                     /translation="MLSTFEQKLDNYASLIVEVGANIQKDQTLFISASIETA"
BASE COUNT         1561 a         1385 c         1297 g         1646 t
ORIGIN      
        1 atcgtcgaaa ccctttcaca cgaagggaag actgtcgtct ccatgaaaga ctcagaaggg
       61 atcctcgctg tcattgccct gaaggatacg cttcgccctg aagccgtcca ggcgatcaat
      121 gatttgaagc aattggggat ccggtcgatc atgttaaccg gcgataatga gaagacggca
      181 aacgcgattg caaaagaagc aggattggat cggtttgcag cggaatgtct gccggagatg
      241 aaagtcgagg aattgaaacg gcttctaaag gaatatggag aggtcggcat ggtcggagac
      301 ggcatcaatg acgctcctgc attagcgacc gccacatctg gcattgcaat gggtgaaggc
      361 acggatgttg ccttggagac agcggatgtc gtccttatgc aaaacgactt gacccgaatt
      421 tcctacgcgg tgaaagtttc gaagaagatg cagcggatcg tgaagcaaaa cgtattcttt
      481 tcgatcgctg tcatcgccgt cctgatcatc tccaatttca tgcaagtcgt cgatcttcct
      541 ttaggcgtca tcggccatga aggaagcacg atccttgtca tcctgaatgg actgcggatg
      601 ttgaataaag tcgattaaag aaaaaagagc aatgcatgtc agctgactgc attgctcttt
      661 tcctttgcta gcttcgcttg cttgcgttct gtcatgaccg cattcaattg tccgcctagc
      721 atcaagatga tcgctgaaaa ataaagccac atcatcagga cgataatgcc cccgatgctt
      781 ccgtacgtct ttgagtaatt tgcaaagctc ccgacgtaaa acgagaacgc aagagatgtc
      841 aagatccagc ctgctgtcgc gaaaagtgcg ccaggcatcg cgcttttcag ccggatcttc
      901 aaattcggga ccgcccaata gagtgcggaa aaaaccagga aaatcaaaat tggcggaatg
      961 acccatctca tgctccccca taatctcagg aacccttcct ccatgccaaa ataggagaat
     1021 gccagcattc cgatctgttg accgaaaaca ggaagaatca atgcgatgat caaaacaccg
     1081 atcaaagcaa tcgtgaatac gacggacatt gcccttgaaa tcaggaaaga ccttgtttcc
     1141 tcgacataat aagaacggtt caatgccttc gtaagagcat tcatcccttt ggaggcggac
     1201 cagatcgttg ccaatgcccc gatggataat agtcccccgt tccggttttt caatatttcc
     1261 tcgatggtat cgtgaataag tgtatagacg ctatcaggcg cataatccct tatgaacagg
     1321 aatacttggg attcatcgag attcaaaaaa ggcaatagtg taattaagaa gattaataga
     1381 ggaaataaag ataataggaa aaagaacgcc aactgagaac caagtccagt gatatccact
     1441 ttcttgattc tcacgaggag ctccttccaa aatccattcg atgttgtcac atcgaatttc
     1501 tccaaatctc catccttgac atcatcaaag aatttcatga ttttgttatt tgccaggaat
     1561 ccgctttttt cttcttctgt ttgtcgtttt tcttttttcg gaggtaacgg gtctttatgc
     1621 ttcacattcg actcactcct tccgtctatt acatcttttt tgtttccata ccttctttcg
     1681 attcgttgac aatttccttg tactcctctt tcgaatcaac aaaggcttcc ttcgtgccga
     1741 cgacaagttc tttcacttga ggagtcaacg tctttaattc ctcgacttga ttcttaatat
     1801 atgccgcatc gcccgaaaat tgttcgtaaa tggataagta cttgtctttc tgctgttgaa
     1861 gtttctgttt caagacatcc ggattttttg aataataccc tacttgctca atcatcgcgc
     1921 ttgattttct cttcacctgg taacgggtgc ttctatcgaa catgctgatg gctccaccca
     1981 atattgcccc caacatgata aaccttccaa atttgctgtt tcccacgtaa aaccctccaa
     2041 tatcattcta atttgcatat ctcattatac ccctcggatg aaaaaacaaa caatcttccc
     2101 gatttcgcaa aaaactcttg actaaagtga gaaatttgag gaagataatg cgtaacacta
     2161 tcaatattcc caatagtcta atgtgttaga tagtgccgat gaaagggagt caatagtccg
     2221 ggaagctatt gatgggagag ggaatttttt taaaaaaaag acaaactaaa caagggggta
     2281 gtaggatgga tgcaatcaca agctttttgg acaaggcaag cggatttatt tggggaccgc
     2341 cacttcttgt cctacttgtg ggaacgggta tctttttaac tgtaagactc ggatttatcc
     2401 aagtgcgttt attgccgtat tctttgaagc aagtattttc ccgtaagcac agtaaaaagg
     2461 ctgaggggga catttcacaa ttccaggcgc tgatgaccgc aatggccgcc acagtcggtg
     2521 taggtaacat cgtcggtgtc gcaaccgctg tttacatggg cggaccgggt gcaatcttct
     2581 ggatgtggct cgcaggtttc ttcggaatgg cgactaaata tggggaagcc attctagcgg
     2641 taaaatatcg tgtcaaggac tccaaaggcg aaatggccgg cggaccgatg tattatttgg
     2701 aacacggttt aaaacaaaaa tggctaggtg tcgcatttgc gatatttggt gctctcgctg
     2761 cattcggtat cggtaacggt acacaatcga aggcagtcgc agacgttatg aaaagcacat
     2821 tcaatgtgcc gcatttggtt acgggtattg cactgttaat cttcggtgca ctcgtcatcc
     2881 ttggtggaat taaatctatc ggacgggtta cggcattctt cgtaccaatc atggcactct
     2941 tctatttcat cgcgggtgca atcgtgatga tcatgaacat cgaactcgtc ccggcagctt
     3001 tcggtttgat tttcagtgac gcatttacgg ggcaagccgc tgcaggcggt gccatcggtg
     3061 cagttatccg tttcggtgtt gcccgtggtc tattctcaaa cgaagccggt cttggatctg
     3121 ctccgattgc ggctgcagct gcaaaaacgg atatgcctgg ccgtcaagcg ctcatttcca
     3181 tgacgcaagt attattcgat acaattatca tctgttcaat tacaggtgtc acaatcgtca
     3241 tgtctggcca atggaaagac aaaacgattg acgcaggtgc attgactgca caagctttcg
     3301 gtgaattcct cggtggagcc ggcccgatca tcgttgcaat cggtatcgtg ttcttcgcga
     3361 catccacaat tcttggatgg ggttattatg gtgagaaatg cttccaatac ctattccctt
     3421 caaacggagc agtattgggc taccggattg cgttcgtcgc gttcatcatt gtcgggtctc
     3481 ttgtatccct cgacatcgta tggttgcttg cggacgtatt gaatggttta atggcaattc
     3541 ctaacttgat cggtcttctt gggttatctg gtgtgatcgt tatggaaaca aaacgattca
     3601 gagcgaagat tgctgaagaa aaagaagcgg aaaagatggg gaagacagca cctgttgaca
     3661 gcactgtcga ttaatgtgat gattaagtcc gaaagctaat gaagggcgga cgacattatg
     3721 gatctatctc aaccaacacc tgaaaatgta acgtttatga tcgaaaagat caaagagaaa
     3781 ctgcggatgg tcaatgtgga tgcaatgaaa gcggagaact tcaacacttc ccaatatgaa
     3841 gatctcgttt atatatacga aatggtcatg aaacgggata cgatcactcc gaatgaaatg
     3901 caagccattg ctgctgaact aggttctctt agaaaatgaa taatgaaaag ccgatgcaac
     3961 aggattttcc tgaagcatcg gctttttact tgattctatt ctactgatgt cccgtctttt
     4021 aggacaagtg gctgaataag cacttcaact cgtctgttct tgcttcttcc ttctgccgtg
     4081 tcattggaag cgacaggttt attttcacca tagcctttga cgctgaacag ctggggatcc
     4141 agttggtcat tgctgacgac ggttttcagg aaattgattg cccgcatcat gctgagctcc
     4201 cagttcgatg ggaattccgc gctattgatc ggaacgttgt ctgtatgtcc cgtaatgaca
     4261 atttgacgcg gtttgtcaaa gacaagcaga ttggcgatat ctttcgataa tgttacgtat
     4321 tgcggtttga tcgtcgcttt gcctgattcg aataggatgc tgtccctgat tgtgatcagc
     4381 agcccctctt cagtcaattt cgtctggaac tgattttcaa gctcatgaac cgcgatatat
     4441 tcatccagct tgtctcggat ttcctcaagc gattgctggt cttccaaata ggaggagttt
     4501 tcattttcct cccccgcgct gtctttcgga atcggtaccg gagtcggtgc actgttgtcc
     4561 attacacctg tccctccgtc aaaaatctga ttgaacacgg aagatatctg gctgaattta
     4621 gcttcatcca acgtgctgga tgcgaatagg acgataaaca tcgctaacag caatgtaaga
     4681 aggtccgcat aaggcaaaag ccaagattca ttgatatgcc cttcttcctt attccttctc
     4741 ttacgtttcc tcgacaacct gccccgcccc cttgtcgctc gtatttttcg ctgcgagctt
     4801 tcgacgttct tccatcgaca agtaagacgc cagtttctgt tcgatcacac gcggcgcctc
     4861 gccctcgagg actgacagga tgccttcaat catcatcatt ttttggcgaa cttcttcctt
     4921 cgatttacgt tgcagtttgt tggcgaatgg atgccatagt acataccccg taaaaatccc
     4981 taacaacgtc gcaacgaatg ccgccgaaat ggcatgtccc aatgcatcga tgtcattcat
     5041 atttttcaaa gcggcaatca aaccgacaac cgcccccagc actccgagtg tcggcgcgta
     5101 tgtccctgct tgcgaaaaga tttgcgcacc agccgaatgc ctgtcctcca ttgcgtccac
     5161 ctcttcggta agcacatcgc ggatataatc ggcattttgt ccgtcgattg ccagcccaag
     5221 tccatttctt aaaaacggat cagtgatctc atcggctttt gcctctaaag acaataagcc
     5281 ttcacgccta gcaacatctg cccaccccga aaacatcttg atcaaatcgg aatcggcagg
     5341 caaattctgt tccctgaaaa tgatgccgaa cagctttggc acccgcttga tctccttcat
     5401 cggaaatgca atgacaactg ctgccattgt gccgacaatg ataatgagaa tcgcagctat
     5461 atttaacaga ttatccggtg tgacgccctt catcgccata ccgacaaaaa gggccaccat
     5521 tccaaggata agtccaaaaa gggttgataa atccatataa gtacctccaa gtcctactgt
     5581 ctacttcttc ttatttcgac ataatcccat ctttctttag tcctcttcaa taataaatat
     5641 caatcttcaa tgaaatgtca cctatagtac acatattccc gcattattga ttactgagga
     5701 ttttcgcatt tttccctttg cctgttatct gacactggag tactattaat acatatcgtc
     5761 taaggagggt tttatgttgt cgacatttga acagaaattg gataattacg ccagtctgat
     5821 cgtggaagtc ggagccaaca tccaaaagga ccaaacgctc ttcatcagcg cttccattga
     5881 aacggctga
//
LOCUS       DASULI010000132         4061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_148600, whole
            genome shotgun sequence.
ACCESSION   DASULI010000132 DASULI010000000
VERSION     DASULI010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148600"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /gene="aroC"
                     /locus_tag="VFX34_02905"
     CDS             <1..824
                     /gene="aroC"
                     /locus_tag="VFX34_02905"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5563885.1"
                     /translation="KYGHRDLRNVLERSSARETTMRVAIGAVAKQFLRELNIQTVAHV
                     TEIGGITTDPKTYSELSIDELRQVIENDPVYCADTKASTRMVAAIDDIKDRGDTLGGV
                     VEVIVEGCPPGIGSYVQFDRKMDGKLAGAMMSINAFKGVEFGLGFKMAQKPGSEVHDE
                     ISWSKELGYYRKSNRLGGLEGGMTTGMPIVIRGVMKPIPTLYKPLESVDIDTKEPFVA
                     TIERSDPCAVPAASVVAEHVIATEMAKAIMEEFRSDTMDGLKEEIERYRQYVKEF"
     gene            826..1932
                     /gene="aroB"
                     /locus_tag="VFX34_02910"
     CDS             826..1932
                     /gene="aroB"
                     /locus_tag="VFX34_02910"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5563886.1"
                     /translation="MGKLTVQLPTMQYDVHIGEDVYDLFSSDYKGLLNSADRVAIIAD
                     EQAADLHLPVLLDALRKVDIDPLVKIVPPGEKCKTQEVYFECQSYLVRENFTRNSLLI
                     AFGGGACGDLTGFVAATYMRGIKFVQCPTTILAHDSAVGGKTAINLPEGKNMVGSFHQ
                     PSAVLFNTKLLATLSPREIRSGMAELIKHAFISDAQWSESLLKEERFSNPAEEWLAKE
                     LLKGIQVKANIVAEDEYEHSTRKFLNFGHTFGHAVEAVCGFGGLSHGECVMIGMAYSF
                     ILSEYHGNMDASFTCRFIKFARRNGYSFAPILEHSFGSFLVFMEKDKKAAFGEMNFVV
                     LEKLGIPFVKKVSAEECKQVFFELQKRIEMGERS"
     gene            1929..2294
                     /gene="aroH"
                     /locus_tag="VFX34_02915"
     CDS             1929..2294
                     /gene="aroH"
                     /locus_tag="VFX34_02915"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5563887.1"
                     /translation="MTVRGVRGATTVASDQSDIVLEATRTLVIEMAEENGIVPEEIIS
                     VIVSTTRDIESAFPAKAVRTLDGWKYVPVMCTHEMDVPGSMPLCIRILMHVNSETHQK
                     DIKHIYQNEAVQLRPDLQK"
     gene            2321..3415
                     /gene="hisC"
                     /locus_tag="VFX34_02920"
     CDS             2321..3415
                     /gene="hisC"
                     /locus_tag="VFX34_02920"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5563888.1"
                     /translation="MQWKKVLSAMKPYKPGRSIEEVKKAYHLDSVVKLASNENPYGCA
                     SSVSEYLSSASFPFEIYPDGHGATLRTKLAEKHGVDESSILFGNGSDEIIAIISRALL
                     DEKVHTIIPIPSFPQYGHNAKIDGADITEIPLKEGRHDLNDFIEAINDRTAVIWVCNP
                     NNPTGSLIPHDELVSFLERVPENILVVLDEAYFEYVTDSDHVDSITLIDRFPNIIVLR
                     TFSKAYGLASFRIGYAVGSPDSITELNKVRNPFNNNTLAVAVAGKALEDDAFIEKCRK
                     LNEIQRKRYKTFAFEKGLHMFDSETNFVLMEVPTDADEASEFLLRHGYIIRSGNALGT
                     PGYVRITIGSEEQNTGLLEAFTLLLQEKGR"
     gene            3418..>4061
                     /locus_tag="VFX34_02925"
     CDS             3418..>4061
                     /locus_tag="VFX34_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX5563889.1"
                     /translation="MNENVAIIGLGLIGGSLALALKRGSRNIHITGFDRSYATADEAY
                     RRGIIDMIAPSAKAACENADYVIFATPVNTTIALMEEALEWKLKQDVILSDTGSTKNP
                     IMQAAERLQEKGLTFIGGHPMAGSHKSGIAAAKGHLFENAYYVLTPSEGASAEQIDKL
                     SDLLAPSKGKIVILDAAEHDRMTAIVSHFPHIIASSLVGRLADQEKDQPFVKNLA"
BASE COUNT         1167 a          842 c         1041 g         1011 t
ORIGIN      
        1 tgaagtatgg gcaccgcgac ttgcggaacg tactggaaag atcttcggcg agggagacaa
       61 cgatgcgtgt tgcaatcggt gccgtagcta aacagttcct ccgtgaatta aacatccaaa
      121 ccgttgcaca cgtgacggaa attggtggga tcaccaccga tccgaagaca tattcagaat
      181 tgtcgataga tgaattgcga caagttatcg aaaacgatcc ggtctactgc gcagacacga
      241 aagcttcaac ccgtatggtt gctgcaatcg acgatatcaa agaccgcggg gatacgctgg
      301 gaggcgtcgt ggaagtcatc gtggaaggtt gccctccggg aatcggaagc tatgtccaat
      361 tcgatcgcaa gatggatgga aagcttgccg gagcgatgat gagcattaat gccttcaaag
      421 gcgtcgaatt tggattgggc tttaaaatgg cgcaaaaacc gggcagcgaa gtgcatgatg
      481 aaatttcatg gtcgaaagag ctgggctact atcgcaagtc gaatcggctt ggcgggctgg
      541 aaggcggcat gacgaccggc atgccgattg ttatccgagg tgtcatgaaa ccgattccta
      601 ctttgtacaa gccgcttgag agcgtggata tcgatacgaa agagccattc gttgctacta
      661 tagaaagatc ggacccttgt gccgtaccgg cggcttccgt cgttgcggag catgtcattg
      721 caactgaaat ggcaaaagcg atcatggagg aattccgttc ggatacgatg gatggcttga
      781 aggaagagat cgagcggtac agacaatatg taaaggagtt ctgacatggg gaaacttacc
      841 gttcagttgc caactatgca atatgacgtg catatcggtg aagatgtgta cgatttgttt
      901 tcatctgact ataaaggttt gctgaatagc gcagatcggg tagccatcat tgctgatgag
      961 caagcggcag accttcacct gccagtactc cttgatgctt tgcgaaaagt ggatattgac
     1021 cctttggtga agattgttcc tccaggagag aaatgcaaga cacaggaagt gtattttgaa
     1081 tgccaatcct atttagtaag ggaaaacttc acccgtaact ctcttctaat cgcatttggt
     1141 ggaggggctt gcggggattt gacaggattc gtcgcggcca cttacatgag gggcatcaag
     1201 tttgtgcaat gccctacgac catacttgct cacgacagtg cggtaggcgg gaagactgcc
     1261 atcaatctgc cggaagggaa aaacatggtt ggttctttcc atcaaccgtc ggccgtatta
     1321 ttcaatacga agctgttagc gaccctgtca cctcgtgaaa ttcgctccgg tatggctgaa
     1381 ttgataaagc acgcattcat ttccgatgca cagtggtctg aatcgctttt gaaagaagag
     1441 cgtttttcga acccagcgga agaatggttg gcgaaagagc ttttaaaagg aatccaagtg
     1501 aaggcgaata tcgtcgcaga agatgaatac gagcattcaa caaggaagtt tttgaatttc
     1561 ggccatacat tcggccatgc agtagaggcg gtttgcggct tcggtgggct gagccacgga
     1621 gaatgcgtca tgattggcat ggcttacagc ttcatcctaa gtgaatatca cggaaacatg
     1681 gacgcatctt ttacctgccg cttcatcaag ttcgctagaa gaaatggata ttcgtttgcc
     1741 ccaattttgg agcattcttt cggaagtttc cttgtattca tggaaaaaga taaaaaagct
     1801 gcattcggcg aaatgaattt cgttgtattg gaaaagctag gcattccctt tgtgaagaaa
     1861 gtttcggcgg aggagtgtaa acaggttttc tttgaattgc agaaaagaat cgaaatgggg
     1921 gaacggtcat gacagtaagg ggagtaagag gggcaactac cgttgcctcg gatcagtctg
     1981 atatcgtatt ggaagctaca aggacattgg tgattgaaat ggcagaagaa aatggcatcg
     2041 ttccagaaga aatcatttcg gtcatcgttt caactacacg ggacatcgaa tcggcatttc
     2101 ctgcaaaagc agtcaggaca ctggacgggt ggaaatacgt acctgtcatg tgtacacatg
     2161 aaatggacgt tccgggatca atgccgctat gcatccggat tctcatgcat gtcaattccg
     2221 aaacacatca aaaggatatc aagcatattt atcaaaacga agccgttcag ctacgaccag
     2281 atttgcaaaa ataagcgaca tgcagaccgg aggaatacat atgcaatgga aaaaagtttt
     2341 atctgccatg aagccttata aaccgggcag atcgattgaa gaagtgaaaa aggcatatca
     2401 cttggacagt gtcgtgaaat tggcttcaaa tgaaaatcca tacggctgtg cctcatccgt
     2461 atcagagtat ttaagtagtg cgtcgttccc gtttgaaatt tatccggatg gccatggtgc
     2521 aacccttcgg acaaaactgg cggaaaaaca tggtgtggat gaatcgtcaa tcctcttcgg
     2581 caatggttca gacgaaatca ttgcaatcat ttcaagagct ttgcttgatg aaaaagttca
     2641 tacaatcata cctataccat cttttccgca atatggccat aatgcaaaaa ttgatggagc
     2701 ggacataacg gaaatccctt taaaagaagg acgtcatgat ttgaacgatt ttatcgaagc
     2761 gatcaatgac cggaccgcag tcatctgggt gtgcaatccg aacaatccga ccggcagtct
     2821 aattccgcac gatgaattgg taagcttttt ggagcgtgtt cctgaaaata tccttgttgt
     2881 cctggatgaa gcctattttg aatacgtaac ggattccgat catgttgatt ctattacact
     2941 tatcgatcgt tttcctaata tcatcgtctt gcgtacattt tcaaaagcgt acggacttgc
     3001 atctttcaga atagggtatg cggtcggttc acccgatagt atcaccgagc tgaataaagt
     3061 gcgaaaccct ttcaataaca atacactcgc tgtagctgtc gcgggcaagg cgttggaaga
     3121 tgatgcattc atcgagaagt gcaggaagct gaatgaaata cagaggaagc gttacaagac
     3181 attcgctttt gaaaaaggac ttcacatgtt cgattcagaa acgaactttg tgttgatgga
     3241 agtgccgacg gatgctgatg aagcttcgga attcctcctc cggcacggtt atatcatcag
     3301 aagcggcaat gcactcggaa cacccggata tgtcaggatt acaatcggtt cggaagagca
     3361 gaatacaggg ttgcttgaag cgtttactct tttattacaa gaaaaaggta gataacaatg
     3421 aatgaaaatg tagcaattat aggcttgggt cttattggcg gctccctcgc tcttgcattg
     3481 aagcgaggca gccgcaacat acatataacg ggctttgacc gttcatacgc aacagctgat
     3541 gaagcatacc gacgggggat tattgacatg attgcccctt ctgcaaaagc tgcatgtgaa
     3601 aatgccgatt acgtcatctt tgcgacgccc gtgaatacga cgatcgcctt aatggaagag
     3661 gctttggaat ggaaactaaa acaagatgtc attctatcgg atacgggaag cacgaaaaat
     3721 ccgatcatgc aggccgccga aagacttcag gaaaaggggt tgacgttcat aggcgggcat
     3781 ccgatggccg gttcccataa gagtggaatc gcagctgcaa aagggcatct gtttgagaat
     3841 gcgtattatg tattgacacc ttctgaaggt gcaagcgctg aacaaattga taagctatcc
     3901 gacctgttgg ccccttcaaa aggcaaaatc gtcatattgg acgcagcaga acatgatcgg
     3961 atgacggcga tcgtaagtca cttccctcat attatcgcgt cttcacttgt cgggaggctg
     4021 gcggatcagg aaaaagatca gcctttcgta aaaaatcttg c
//
LOCUS       DASULI010000133         5014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1078308,
            whole genome shotgun sequence.
ACCESSION   DASULI010000133 DASULI010000000
VERSION     DASULI010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078308"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..1690)
                     /gene="rho"
                     /locus_tag="VFX34_02930"
     CDS             complement(407..1690)
                     /gene="rho"
                     /locus_tag="VFX34_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5563890.1"
                     /translation="MSILTIASLENMTLKELYELAREYKISYYSKLTKKELIFAILKT
                     RAEQEGFFFMEGVLEIIQSEGYGFLRPINYSPSSEDIYISASQIRRFDLRNGDKVTGK
                     VRPPKENERYYGLLQVEAVNGEDPEVARERVHFPALTPLYPDRHIKLETTPQKLSTRI
                     MDLVSPVGFGQRGLIVAPPKAGKTMLLKEIANAITTNHPEAELIVLLIDERPEEVTDI
                     ERSVNADVVSSTFDEVPENHVKVAELVLERAMRLVEHKRDVVILMDSITRLARAYNLV
                     IPPSGRTLSGGIDPAAFHRPKRFFGAARNLEEGGSLTILATALIDTGSRMDEVIYEEF
                     KGTGNMELHLDRSLAERRIFPALDIRRSGTRKEELLIPSEQLEKLWAIRKTFSDAPDF
                     AERFLKKLRQSKSNEEFFDKLNEEMKAHRNGKGLL"
     gene            complement(2010..2975)
                     /gene="glpX"
                     /locus_tag="VFX34_02935"
     CDS             complement(2010..2975)
                     /gene="glpX"
                     /locus_tag="VFX34_02935"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5563891.1"
                     /translation="MERSLSMELVRVTEAAAVAASRWMGRGLKNEADDAATEAMRTVF
                     DTIPMQGVVVIGEGEMDEAPMLYIGEELGTGQGPEVDVAVDPLEGTNIVASGGWNALA
                     VIAIADKGNLLNAPDMYMNKIAVGPEAVGKIDIDASVTDNLHAVAKAKNKSIDEVVAI
                     VLNRERHSKIIEEIRAAGARIKLINDGDVAAAINTAFDDTGVDILFGLGGAPEGVIAA
                     VGLKCLGGEIQGRLVPSNEEERLRCEKMGIDVNKVLYMDDLVKGDDAIFAATGVTDGE
                     LLRGVQFKGGYSETHSLVMRSKSGTIRFVEGRHSMKKKPNLVMQD"
     gene            complement(3076..4353)
                     /locus_tag="VFX34_02940"
     CDS             complement(3076..4353)
                     /locus_tag="VFX34_02940"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5563892.1"
                     /translation="MDVYKIRGGEPLRGTIKVSGAKNSAVALIPASILADSPVTIEGL
                     PEISDVHTLQALLEDIGGKVEFDAGKMTIDPSEMIAMPLPNGNVKKLRASYYMMGAML
                     GRFKHAAIGLPGGCHLGPRPIDQHIKGFEALGAKVTNEHGAIYLRADELRGAKIYLDV
                     VSVGATINIMLAAVRAKGRTIIENAAKEPEIIDVATLLSNMGANIKGAGTNVIRIEGV
                     DRLHGTKHTIIPDRIEAGTFMIMAAAVGDGITIDNVIPFHVEALTAKLREMGVKVEER
                     EEHIFIPKTENLMAVDVKTLVYPGFPTDLQQPFSVLLTQANGSSVITDTIYSARFKQI
                     DELRRMNADGKVEGQSVILSGPTPLQAATVRATDLRAGAALIIAGLLAEGETEIQEIQ
                     HIERGYSSIIEKLRGIGADIRKESVPAKAAISE"
     gene            complement(4374..>5014)
                     /gene="fsa"
                     /locus_tag="VFX34_02945"
     CDS             complement(4374..>5014)
                     /gene="fsa"
                     /locus_tag="VFX34_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX5563893.1"
                     /translation="FIDTANFEEIKEAHSWGILSGVTTNPSLVAKENISFHDRLREIT
                     SLVPGSVSAEVIALDAEGMIEEGRELAKIAPNITVKLPMTPEGLKACSVFAQEGIKTN
                     VTLIFSANQALLAARAGATYVSPFLGRLDDIGQEGIHLISTIADIFTIHDMDTQIIAA
                     SIRSPQHITDAALAGAHIATTPFNVLKQLFNHPLTDKGIQQFLEDWEARKNK"
BASE COUNT          967 a         1303 c         1332 g         1412 t
ORIGIN      
        1 cgcgggcggt ccgtgagcca atcggacaac ggagagtccg atttgccgta tttctgtggt
       61 ttttacagaa atttatgcat cctgctcgca acgctccgct tttactccct tagggtcgca
      121 ggaaacattg ctcgcaaacg ccgttcttcg tgacggcgct tgcaactatt ttatttcgaa
      181 tatcatactg cgatttccgc tccaggcgga cgctttccgc gggcggtccg tgagccaatc
      241 ggacaacgga gagtccgatt tgccgtattt ctgtggtttt tacagaaatt tatgcatcct
      301 gctcgcaacg ctccgctttt actcgcaaaa gccgttctac gtgacggctt tcgctggagt
      361 cgccgccttc cgctatattc aactcagttc aattaaaatg aaaaattcaa agcagccctt
      421 ttccattgcg atgagctttc atttcctcat tgagcttgtc gaagaattct tcgttcgatt
      481 tcgattggcg tagttttttc aggaagcgtt ctgcgaaatc gggggcatcg gagaacgtct
      541 tccggattgc ccaaagtttt tcgagctgtt cggacgggat gagcaattcc tctttccgcg
      601 ttccggagcg tcggatgtcg agtgcaggga aaatacggcg ttctgcgagg ctgcggtcaa
      661 gatgcagctc catattgcct gtccctttga attcctcgta aatgacttca tccatccgcg
      721 agcctgtgtc gatcaaagcg gttgcgagaa tcgtcaagct gccgccttcc tcgaggttcc
      781 gcgccgcacc gaagaagcgc ttcggacggt ggaatgccgc cgggtcgata ccgcccgaaa
      841 gcgtacggcc gcttggcgga atgacaaggt tgtaggcgcg cgcaagtctc gtgatcgaat
      901 ccatgagaat gacgacatcg cgcttatgtt cgacaaggcg catggcgcgt tcaaggacga
      961 gctctgcgac tttcacgtgg ttttccggca cttcatcgaa tgtcgagctg acgacatcgg
     1021 cattgacgga tcgttcgatg tccgtcactt cctcagggcg ctcgtcgatg aggaggacga
     1081 tcagttccgc ttcgggatga ttcgtcgtga tggcgttggc gatttccttc aacagcatcg
     1141 tcttccctgc tttcggaggt gcgacgataa gtccacgctg gccgaatccg accggcgaca
     1201 ctaagtccat gatgcgggtc gagagtttct gtggtgtcgt ttcaagtttg atatggcggt
     1261 ccgggtaaag cggcgtcagt gccgggaagt ggacgcgttc acgtgcgact tccgggtctt
     1321 ccccgttgac ggcttcgact tgcagcaagc cgtaatagcg ttcgttctct ttcggcggac
     1381 gaacttttcc tgtcaccttg tccccgttac gcaagtcgaa tcgtctgatt tgagaagcgg
     1441 aaatgtagat gtcttcagag cttggcgagt agttgattgg acggaggaac ccgtatcctt
     1501 ccgattggat gatttcgagc acgccttcca tgaagaagaa gccttcctgt tcggctctcg
     1561 ttttcaggat ggcgaaaatc agctcttttt tcgttagttt gctgtaatag gagattttat
     1621 attctcttgc aagctcatat aactctttca gggtcatatt ttccaagctg gcgattgtta
     1681 gaattgacat ggtattgtat gctccactct ttatttgttt ttcgtttgtt agggtttgtt
     1741 ttcattcggg taggtgaatc ggtcaataga aggatgcccg gcggatagcc gggcgttgag
     1801 gcttgttatt ttttatctaa aattaacgtc tagactccgg gtggcagaag ctcgggtcat
     1861 aagccaaagc tgctgtgtgg caaaaagcgc cacttcgcat cttcgtctta tgcctgaggc
     1921 cggcaggaag ccggtcatgc agtcgttgcc gcaggacgcg gcgcacttag actgcgaacc
     1981 tctgctaacc gccaccctac gcttttgtct taatcctgca ttaccaaatt cggttttttc
     2041 ttcatactgt ggcgtccttc tacgaaacgg atggttccgg atttggagcg catgacgagt
     2101 gagtgggttt cgctgtagcc gcctttgaat tggacgccgc gcagcaattc gccgtctgtc
     2161 acgcctgttg ccgcgaagat ggcgtcgtcg cctttgacaa ggtcatccat atagaggact
     2221 ttgttgacgt cgattcccat tttctcgcag cggaggcgct cttcttcatt tgaagggacg
     2281 agccggcctt ggatttcccc gccgaggcat ttgagtccga ctgcggcaat gacgccttca
     2341 ggagctccgc cgagtccgaa caggatgtct acgcccgtgt cgtcgaatgc cgtattgatg
     2401 gccgccgcca cgtctccgtc gttgataagc ttgatgcgtg cgccggcagc tctgatttct
     2461 tcaatgattt tcgaatggcg ttcacggttg aggacaattg ccacaacttc gtcgatcgat
     2521 ttgtttttcg ctttggcgac ggcgtgtaga ttatccgtga cggaagcgtc gatgtcgatt
     2581 ttgcctaccg cttccggtcc tactgcgatt ttgttcatgt acatgtcagg tgcgttgaga
     2641 aggttccctt tatccgcgat tgcaatgact gcaagcgcat tccagccgcc tgatgcgacg
     2701 atgttcgtgc cttcgagcgg gtcgacagcg acgtccactt ccggaccttg gccagttcca
     2761 agctcttcgc cgatatagag catcggtgct tcgtccattt cgccttcgcc gatgacgacg
     2821 accccttgca tcggaatcgt gtcgaacaca gtacgcatcg cttccgtcgc ggcgtcatcc
     2881 gcttcgtttt tcaaaccgcg ccccatccag cgggatgccg ctacggccgc cgcctctgtt
     2941 acacgtacta attccatcga taaactgcgt tccattatga ttgttcctcc cgtttagcgc
     3001 atttcatttt ttattgacaa ataataaacg ctattcatta gctgtaagat tcgtttctag
     3061 tatatcacaa catttttatt cactgatggc cgcttttgca ggtacggatt ctttccggat
     3121 gtccgccccg atgccgcgga gtttctcgat gatcgagctg tagccccgtt cgatatgttg
     3181 gatttcctgg atttccgttt cgccttcagc gagcaggcct gcaatgataa gcgccgctcc
     3241 ggcacggaga tccgtagcgc gtacagtcgc cgcttgaagt ggtgtcggtc cagaaagaat
     3301 gactgattgc ccttccactt tcccgtcggc attcatccgt cgtaattcat cgatttgctt
     3361 gaaccgcgcg gaatagatcg tatcggttat gacggaggag ccgttcgcct gcgtcagcag
     3421 tacggaaaat ggctgctgca ggtcggtagg gaagcccggg tagacgagcg ttttcacatc
     3481 gaccgccatc aaattctccg ttttcggaat gaagatatgc tcttcccttt cctccacttt
     3541 gacgcccatt tcacgcaatt tcgcagtcag cgcttcgacg tggaaaggga tgacattgtc
     3601 gattgtgata ccgtctccga ccgccgctgc catgatcatg aacgtgcctg cctcgattct
     3661 gtccgggatg atcgtatgct tcgttccgtg cagccgatcg acaccttcga tgcggatgac
     3721 gttcgttcct gcacctttaa tgttggcgcc catattcgaa agcaatgtgg cgacatcgat
     3781 gatttccggt tctttcgccg cgttctcgat gatcgtccgc cctttggcac ggactgccgc
     3841 aagcatgata ttgatggttg cgccgaccga gacgacgtcc aaatagattt tggcgccccg
     3901 taattcatct gcacgcaaat agatggcgcc gtgttcgttt gtcacttttg cgcccaaagc
     3961 ttcgaaacct ttgatgtgct gatcgattgg acggggccct aaatggcagc cccccggaag
     4021 tccgattgcc gcgtgtttga agcgtccgag catcgcaccc atcatgtaat acgatgctct
     4081 cagctttttc acgttgccgt tcggaagcgg catcgcaatc atttccgacg ggtcgatcgt
     4141 catcttgccc gcatcgaatt ccactttccc gccgatgtct tcaaggagcg cttgaagtgt
     4201 gtggacgtcc gaaatttccg gaagcccttc gatcgtaacg ggtgaatctg ccaagatgga
     4261 agctggaatc aacgcgacgg cactgttttt cgctccgctc acttttatcg ttccacgtag
     4321 aggttcgccg cctctgattt tataaacgtc catttgagtc tcctttaggc gtttcacttg
     4381 ttctttcttg cttcccaatc ttcaaggaat tgctggatgc ctttgtcggt caacggatgg
     4441 ttgaataatt gcttaagcac gttgaatggc gtcgtcgcga tgtgcgctcc tgcaagggcc
     4501 gcgtctgtaa tatgctgcgg gctgcggatg gaggctgcga tgatttgcgt atccatgtcg
     4561 tggatggtga agatgtccgc gattgtgctg atcagatgga ttccttcttg accgatatca
     4621 tcgagtctgc cgaggaatgg ggatacataa gtggctccag cgcgcgctgc aagcaatgct
     4681 tggttggcac tgaaaatgag ggtcacgttt gttttgatgc cttcttgtgc gaaaacggag
     4741 catgctttca ggccttccgg cgtcattgga agtttgacgg tgatgttcgg tgcgattttc
     4801 gcgagttcgc ggccttcttc gatcatgcct tccgcgtcga gtgcgatgac ttctgcgctg
     4861 acagaaccgg gtacgagtga agtgatttca cgcagtctgt cgtggaatga aatgttttcc
     4921 tttgcgacga gtgatgggtt tgtcgttacg ccggatagga ttccccagct atgtgcttct
     4981 tttatttcct caaagtttgc cgtatcgatg aaaa
//
LOCUS       DASULI010000134         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_584809, whole
            genome shotgun sequence.
ACCESSION   DASULI010000134 DASULI010000000
VERSION     DASULI010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584809"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFX34_02950"
     CDS             <1..626
                     /locus_tag="VFX34_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5563894.1"
                     /translation="GLARIEGQPVGIIANQPKVKGGVLFVDSADKAAKFINLCDAFSI
                     PLLFLADVPGFMIGTKVERAGIIRHGAKLIMAMSSATVPKISVVVRKAYGAGLYAMAG
                     PAFEPDVCIALPTAQIAVMGPEAAVNAVYSNKIEAIEDPKEKIAFVQAKHKEYKEEID
                     IYKMASELIIDEIIAPSDLRNVLADRFRFYSTKELPLPPKKHPVYPV"
     gene            691..1581
                     /locus_tag="VFX34_02955"
     CDS             691..1581
                     /locus_tag="VFX34_02955"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5563895.1"
                     /translation="MDVVVAGAGSIGLLIGSYLADAGMDVVFLVRREEQAAVIREQGI
                     RRVSGDGSEKVMKADAQTDAGKLPQNAPWIIATKYDGVAPTVELMLERNLENAVMFVQ
                     NGYGHFDYVRKTAMPNVFFATVEHGAGRLDDRTVSHNGVGALRIAPCRGDEKSFDFLK
                     SVDTPDFPVTFANDAHQLVLRKVLINCMINPLTAILQVRNGELVENKNAKKLFDQLFD
                     EIMAAFPEMQLDLPKSAVEEICVRTAKNKSSMLTDRLNGNPMEIDTVISSLLQMGEGR
                     GVHLPLLQTLENILLAIDRS"
     gene            1588..1980
                     /locus_tag="VFX34_02960"
     CDS             1588..1980
                     /locus_tag="VFX34_02960"
                     /inference="COORDINATES: protein motif:HMM:NF023305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 family protein"
                     /protein_id="HEX5563896.1"
                     /translation="MVNGLLSILTGIIVLFPFLITIIFLVVMRKMGRAPAAVIGKAAD
                     FTTPFLFLSVYITSWSIFGKGTGFYITSIAILVAIFFAVVERVREKEFQIGRVLQKTW
                     RLYFLLLSLVYVVLLFLGIGFKISEYVK"
     gene            2074..>3642
                     /gene="bshC"
                     /locus_tag="VFX34_02965"
     CDS             2074..>3642
                     /gene="bshC"
                     /locus_tag="VFX34_02965"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEX5563897.1"
                     /translation="MEIKSMELSNGNKVMQAYNHDEQFIHTFFDYRNESRSYPERLKE
                     LSGRPFHRDELSSIIRSFMEPFGISEKASVHLEELSANGVVVIGGQQAGILTGPLYSV
                     HKAITVILLAEEKRKELGVPVIPVFWVAGEDHDLNEINHVYTEAIGGVTKHRYPEKFI
                     LKLMASDAAYSHNEMSAFVEGIFRQFGETEHTRTLLEEVLTAVELEKTFTGFFVRLMN
                     GLFREQGLLFIDSAYKPLRQFEKDHFAQFISQSDRLARLIVEKEEQFSELGFGKPIEA
                     ENEAAHLFYVHETGRVLLSKKDGEFVNESTGIRFTREEILNIAAEKPWLLSNNVATRP
                     IMQDFVFPVLAFVGGPGEIAYWALLKEAFHLFGIRMPIIVPRVSMTLVSKRAKQAMET
                     TSLTLEDVLAGKVQEVREQFIDSLQNERFEKLLAETEMQLKGKYEELTKVSSTAMRQL
                     LEKNLKFHEKQLEFIRKKAEEELLIQHETALRKYSILENELFPEGSLQERIYTPYLFM
                     NQYGPDLVQDLIRQS"
BASE COUNT         1010 a          739 c          926 g          967 t
ORIGIN      
        1 cagggcttgc gcggattgaa gggcagccgg tagggatcat cgcgaaccag ccaaaagtga
       61 aaggaggcgt cctattcgtt gattcggcgg ataaggcggc caaattcata aacctatgcg
      121 atgcattttc gattccgctg cttttcttgg cggatgtccc gggcttcatg atcggaacga
      181 aagtggaacg cgccggcatt atccgccacg gtgcgaaatt gatcatggcg atgagctcgg
      241 cgacggttcc taaaatttct gtcgtcgtcc gaaaagctta tggagcaggg ctttatgcaa
      301 tggcgggccc ggcattcgag cctgatgtct gtattgcatt gccgactgca caaatcgcag
      361 tgatgggacc ggaggctgcg gtcaatgcgg tttattcaaa taaaatcgaa gcaatcgagg
      421 atccgaaaga gaaaatcgct tttgtccagg cgaagcataa agaatataaa gaggaaatcg
      481 atatctacaa gatggcctcc gaattgatca tcgatgaaat cattgcacct tcggacttgc
      541 ggaacgtgct tgcggatcgt ttccgttttt attcgacgaa agaacttcca ttaccaccta
      601 aaaaacatcc tgtatatcct gtataatcaa atataaggaa aacaaagggc gcctttccta
      661 aggtcgccct ttttaaaaag ggggattttc atggatgtcg ttgttgctgg ggcgggttct
      721 atcggtttgc tcatcggttc ctatttggcg gatgcgggta tggacgtcgt ctttttagtg
      781 agacgggaag agcaggcggc agttatccgg gaacagggga tcaggcgggt cagtggggac
      841 gggtcggaaa aggtcatgaa agcggacgca caaaccgatg ccgggaaatt gccgcagaat
      901 gccccttgga ttattgcaac gaagtatgat ggagtggcgc cgaccgtaga attaatgctc
      961 gaaagaaatt tagagaatgc agtcatgttc gtccaaaatg gatatggaca tttcgactat
     1021 gtgcgaaaaa cggcaatgcc aaacgttttt tttgcgacag tcgagcacgg cgcaggccgg
     1081 ttggatgatc ggacggtctc ccataatgga gtcggtgcct tgaggattgc cccttgtagg
     1141 ggagatgaaa aaagctttga cttcttgaag tcagtcgata cacctgattt cccagtgact
     1201 tttgcaaacg acgcgcatca gctcgttttg cgcaaagtgc tgatcaattg catgatcaat
     1261 cccttgacgg ctattttgca agtgaggaac ggcgaattag tggagaacaa aaatgctaaa
     1321 aagctttttg accagttgtt tgatgaaatt atggccgctt ttcctgaaat gcaacttgat
     1381 ttgccgaaat ctgctgttga agagatttgt gtcaggacgg cgaaaaacaa gtcatccatg
     1441 ttgacggacc ggttgaatgg aaatccgatg gaaatagata ctgtcatatc ttcactcctt
     1501 caaatgggtg aaggtcgcgg agtgcacttg ccattattgc aaacgctcga aaatattctg
     1561 ctcgcgatag ataggagttg attgacgatg gtgaatggac ttctatcaat acttacagga
     1621 atcatcgttc tatttccatt tcttattaca atcatattcc tcgtcgtcat gaggaaaatg
     1681 ggaagggctc cagctgccgt catcgggaaa gcggcagatt ttacgacccc ctttttattt
     1741 ttgtccgttt atatcacctc ctggtccata tttggcaaag gtaccggttt ttatattact
     1801 tccattgcca ttctcgtcgc tatctttttt gctgttgttg aaagggtgag ggagaaggaa
     1861 tttcaaatag gtcgtgtttt gcagaagacg tggagactgt attttctgct gctgtccctt
     1921 gtttacgtcg ttctcctctt tttaggaatc ggttttaaaa tatcggaata tgtaaaatga
     1981 agggcaatca atccagaaga caagcgtata tttttcaatc cggtgattga gaatgatata
     2041 ctctgttcat atcagtaaaa aggggcatca atgatggaaa tcaagtcaat ggaattgagt
     2101 aatggaaaca aagtgatgca agcatacaat catgacgaac aattcataca tacgtttttt
     2161 gattatagaa atgaaagccg gtcctatccc gaaagactga aggagctgtc gggacgtccg
     2221 tttcatcggg atgaactttc ttctatcatc cgttccttca tggagccatt cggtatttca
     2281 gagaaagctt ctgtacatct cgaggaactt tcggcaaatg gagttgttgt catcggcggc
     2341 caacaagccg gcatactgac tgggccgctc tattctgttc ataaggcgat tacggtcatc
     2401 cttcttgcag aggaaaaaag gaaagagctt ggagtccctg tcattcccgt gttttgggtt
     2461 gcaggagagg atcatgatct gaatgaaatc aatcatgtct acacggaagc aatcggagga
     2521 gtgactaaac atagatatcc ggagaagttc atcctgaaat tgatggcctc cgatgctgcc
     2581 tattcccata atgaaatgtc agcattcgta gaaggcattt tccgccagtt cggggaaacg
     2641 gaacatacgc gaacgctgct ggaagaagta ttgactgcag tggaattgga aaagacgttc
     2701 accggatttt tcgtcagact gatgaacggt cttttccgcg aacagggcct tctcttcata
     2761 gattcggcct acaaaccgtt gcgtcaattc gaaaaagacc attttgccca attcatttca
     2821 caatccgata gacttgcaag gttgatcgtt gagaaagagg agcaattttc cgaattagga
     2881 ttcggaaaac cgatcgaagc tgaaaatgaa gcagcccact tgttttatgt acatgagact
     2941 ggacgcgtcc ttctttctaa aaaagacgga gaattcgtaa atgagagcac aggcatccga
     3001 ttcacaagag aagaaatact gaacatcgcc gctgaaaagc cttggctgtt aagcaataat
     3061 gttgcgacac gtcctattat gcaggatttc gtctttccgg ttctggcctt cgtcgggggc
     3121 ccaggggaaa ttgcttactg ggccttattg aaggaagcat tccatctttt cggaatccgt
     3181 atgccgatca tcgtgccgcg ggtatcgatg acattggttt ccaagcgggc aaaacaggca
     3241 atggagacaa cttcattgac gctggaagat gtattggccg gaaaagtcca ggaagtccgg
     3301 gaacagttca tcgattcact tcaaaatgaa cgatttgaaa agctgcttgc cgaaacggaa
     3361 atgcaattga aagggaaata tgaagagctt acaaaagttt cgagtacagc gatgcgtcag
     3421 ttgcttgaaa agaatttgaa attccacgaa aagcaattgg agtttatacg gaagaaggcc
     3481 gaagaggagc tgttgatcca acatgaaacg gctctccgaa aatacagcat cctcgagaat
     3541 gagctgtttc cggaaggctc gttgcaagag aggatttaca ctccatatct attcatgaat
     3601 cagtatggtc cggatttagt gcaggatttg attcgccagt ca
//
LOCUS       DASULI010000135         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_974073, whole
            genome shotgun sequence.
ACCESSION   DASULI010000135 DASULI010000000
VERSION     DASULI010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974073"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFX34_02970"
     CDS             complement(<1..407)
                     /locus_tag="VFX34_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEX5563898.1"
                     /translation="MYQLKDSDVKQLNALALAYMGDAVYEKAIREHLLRSGRVKPHVL
                     HREATRYVSAKSQAAIVWAMLESGFLTEEEQAVFRRGRNTKSGSVPKNTDVQTYNASS
                     GFEAILGYLHLRGNEERIQDILVESIRFIEQPK"
     gene            complement(412..1812)
                     /gene="cysS"
                     /locus_tag="VFX34_02975"
     CDS             complement(412..1812)
                     /gene="cysS"
                     /locus_tag="VFX34_02975"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5563899.1"
                     /translation="MSIQIYNTLTRKKEPFIPMEEGKVKMYVCGPTVYNYIHIGNARM
                     AVVFDTVRKYLEYRGFDVAYVSNFTDVDDKIIKTANELGEEVSELTERFINAYIEDIT
                     ALGCSKADVHPRVTEHIEDIINFIQVLIDKGYAYESEGDVYYRTRKFDGYGKLSHQST
                     DELKIGARIEAGEKKENALDFALWKAAKPGEISWESPWGAGRPGWHIECSVMAREHLG
                     DTIDIHAGGQDLTFPHHENEIAQSEAMTGKQFARYWMHNGYLNIDNEKMSKSLGNFVL
                     VNDIRKKIDPQVLRFFILSVHYRHPINFSEDLVESAKNGLERIRTAYNNVKHRLETSA
                     DLGDHQDVWLHKITECKTDFETAMDDDFNTANGMAAIFELAKLANVYLMEKNTQHIVL
                     EEFLSTLDTLMSVLGLPFGTADELLDEEIDALLQERLEARKNRDFARADEIRDELKEK
                     GIILEDTAQGTRWKRG"
     gene            complement(1809..>2010)
                     /locus_tag="VFX34_02980"
     CDS             complement(1809..>2010)
                     /locus_tag="VFX34_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017186384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX5563900.1"
                     /translation="KDVPPNATVVGIPGTVVRLNGVKVEKLDHHKLPDPVAEKCSSLE
                     REITRLKEELAKLEQGREREWR"
BASE COUNT          448 a          532 c          412 g          618 t
ORIGIN      
        1 tccttcggct gttcaatgaa ccgaatggat tctaccagta tgtcttggat gcgttcttca
       61 tttccacgta aatgcaagta tccgaggatc gcttcaaaac ccgagctcgc attgtatgtt
      121 tggacatccg tattttttgg aacagagccg gatttcgtat ttcttcctcg acggaaaacc
      181 gcttgctctt cttccgttag aaagccactt tccagcatag cccacacgat ggcagcctgg
      241 gatttcgcag atacgtaacg ggttgcctcc cggtgcagga cgtgcggctt tacacgcccc
      301 gaccgcagaa gatgttcccg aatcgccttt tcatagactg cgtctcccat gtaggcaagc
      361 gcaagtgcat tcaattgttt tacgtcgcta tctttcagtt gatacatcag tttatccccg
      421 cttccatcgg gttccttgcg ccgtgtcttc caggataatg cccttttctt ttaattcgtc
      481 acgaatctca tctgctctcg caaaatcacg gttcttccgt gcttctaaac gctcttggag
      541 caatgcatca atttcttcat ccagcaactc gtctgctgtt ccgaatggaa gaccgagaac
      601 tgacatcagt gtatccaacg tggagagaaa ttcttccaac acaatgtgct gtgtgttttt
      661 ctccatcaag tacacattgg cgagtttcgc tagttcaaaa attgctgcca ttccgttggc
      721 cgtgttaaag tcgtcatcca tcgctgtttc gaaatctgtt ttgcattccg taatcttatg
      781 cagccaaacg tcttggtggt cgcctaaatc cgcagatgtt tcaaggcggt gcttgacgtt
      841 gttgtatgct gtgcgaatcc gttccaaacc attcttcgcg ctttcgacaa gatcttctga
      901 gaagttgatc ggatgtcgat aatgaacaga cagaataaag aagcgcagca cttgtgggtc
      961 gatctttttc cggatatcgt ttacaagaac gaagtttcct agcgacttgg acatcttttc
     1021 attgtcaata ttcaagtagc cgttatgcat ccagtaccga gcaaattgct tgcctgtcat
     1081 tgcttcggac tgagcaattt cattttcgtg gtgcgggaac gtcaagtctt gaccgcccgc
     1141 gtgaatatca attgtgtccc ctagatgctc acgtgccatg acagagcact cgatatgcca
     1201 gcccgggcgc cctgctcccc atggactttc ccacgagatt tccccaggct ttgccgcttt
     1261 ccaaagcgcg aagtccaagg cattttcctt cttttctccc gcctcaattc gagccccaat
     1321 tttcagttca tctgtcgatt gatgagagag ctttccgtag ccgtcaaact tacgtgtacg
     1381 gtaatacaca tctccttctg actcataggc atatcctttg tcaatcaaca cttgaataaa
     1441 gttgataatg tcttcaatat gttccgtgac gcggggatga acatccgcct tgctgcaccc
     1501 gagtgctgtg atgtcttcga tgtaggcatt aataaatcgt tccgtcagtt cggatacttc
     1561 ttcacccagt tcatttgccg ttttaataat tttatcgtcg acatctgtaa aattcgatac
     1621 gtacgctacg tcaaatccgc gatattccaa atattttcga acagtgtcga acacaaccgc
     1681 catcctagca tttccgatgt ggatatagtt gtagacagtt ggtccgcaga cgtacatttt
     1741 tacttttcct tcttccatcg gaatgaaagg ttctttcttc cgtgtaagcg tgttataaat
     1801 ttgaatactc atcgccattc cctctctctt ccctgttcta actttgcaag ctcttccttt
     1861 agtcgggtga tttcccgctc caacgaacta catttttccg ctaccggatc tggcaatttg
     1921 tgatgatcca gcttctccac tttcactccg ttgagcctga caaccgtccc tggaatcccg
     1981 acgaccgtcg cattcggagg aacgtccttc
//
LOCUS       DASULI010000136         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1932386,
            whole genome shotgun sequence.
ACCESSION   DASULI010000136 DASULI010000000
VERSION     DASULI010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932386"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFX34_02985"
     CDS             complement(<1..650)
                     /locus_tag="VFX34_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5563901.1"
                     /translation="MEKQSNNLALYILMFNMFIAMAGIGLIIPIMPEFLGTFGVAGKV
                     LGFLIAIFSFAQFIFSPISGDLSDRHGRKKIIIFGLIIYGSAQLAFSLSTELWMLFVA
                     RFFSGMGAAFIVPPMMAFVADITTLEGRGRGMGLLGASMSLGFMIGPGIGGFLSKISL
                     LFPFYFAAGAAIFAAILSIFILPNPAPAVSVGKVLVKKENLFEQLARSTKTSYFVM"
     gene            complement(797..1867)
                     /locus_tag="VFX34_02990"
     CDS             complement(797..1867)
                     /locus_tag="VFX34_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5563902.1"
                     /translation="MKSLIQSAILILLVSGILLFINAELSEGSKSGKGTITVYNWGEY
                     IDPDLIKQFEEETGIKVIYETFDSNEGMMSKIEQGGTAYDISVPSEYMIEMMKEKNLL
                     LPIDYSKIPNIKHIDPYFLDLAFDPGNQYSVPYFWGTVGIAFNPTLLEGQTFKSWDDL
                     WDPSLKQRVILVDSARETIGMGLNSLGYSLNSTDLDELREATDKLKTLSPNVKAVIGD
                     EVTQLMINNEAAVSLTWSGQAADMMYENEDIDYVVPEEGSNLWFDNIVIPKTAKNIEG
                     AHAFINFMLDPEVAAQNADYVGYSTPNLTALDLMDPEVVEDERFYPDEETRSHLEVYK
                     NLGLELLGAYNELFLEFKMDMR"
     gene            complement(1864..2664)
                     /locus_tag="VFX34_02995"
     CDS             complement(1864..2664)
                     /locus_tag="VFX34_02995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563903.1"
                     /translation="MEKLGKLPKLYLAIIFIILYAPIFYLIFYSFNSGGGMSNFEDFT
                     WEHYAAVFEDKRLIIIVLNTIIIALLSALLSTAIGVIGAMAIYFMRNKKMRNAVLSLN
                     NILIVSPDVIIGASFLILFTMIGVKLGFASVLISHIAFSIPIVVIMVLPKLAEMNTSL
                     IDAALDLGASRKDVMTRVILPFIKPGIFAGFFLALTYSLDDFAVTFFVTGNGFSTLSV
                     EIYSMARTGISLTINALSGLIFIITVALVLGYYAISRKSKTPLAGVKK"
     gene            complement(2648..>3154)
                     /locus_tag="VFX34_03000"
     CDS             complement(2648..>3154)
                     /locus_tag="VFX34_03000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563904.1"
                     /translation="KTYAFIGLMGLYGPLNAFLETVGIGKQQILFTDFSFVFVSVYIF
                     IPFMILPIFNALDKLNPALIDASHDLGANSWTTFRRVIWPLTTNGVKSGIQVVFIPAL
                     SLFMITRLIAGNKVITLGTAIEQQFLVTQNWGMGSTIAVFLIVFMFIIMLVTNGKEKG
                     ATDNGKAR"
BASE COUNT          910 a          731 c          689 g          824 t
ORIGIN      
        1 aacatgacaa aataggaggt tttcgtcgac cgtgccaact gctcgaatag attttccttc
       61 ttcacgagca ctttcccaac tgaaacggca ggcgccggat tcggtaaaat gaaaatcgag
      121 agaatcgctg caaatattgc agcccccgct gcaaaataaa atggaaagag caagctgatc
      181 ttagagagga atccaccgat tccaggacca atcatgaagc caagtgacat ggatgcccct
      241 aatagcccca tccctctacc cctgccttcc aaagtagtga tgtctgcgac aaacgccatc
      301 attgggggaa cgatgaacgc ggctcccatg ccggaaaaga accgggcgac gaaaagcatc
      361 caaagttcag tcgacaagct gaatgcaagc tgtgcagatc cgtagatgat taaaccgaag
      421 ataatgattt tctttcttcc atgccggtcg gataaatccc ctgaaatagg agagaaaatg
      481 aattgtgcaa acgaaaagat tgcgataagg aatccgagaa cttttccggc gacgccgaat
      541 gtccccaaaa attccggcat gatcggaatg ataagtccga tgcctgccat tgcgatgaac
      601 atattgaaca tcagtatgta taaagccaag ttatttgatt gtttctccat tgaaggccat
      661 ccgcccctct aaacgatttc gtgttattgc aatatcttac catagaagca tacgaacgct
      721 taatggaaag ccttcatccc ttggcatata aataaacgcc cttaaacggt ttgatcgttt
      781 aagggcgatt ggtgaatcat ctcatatcca tcttgaattc caagaaaagt tcattatagg
      841 cgcctaataa ttcaagtcct aaatttttat acacttccaa gtggcttctc gtctcttcat
      901 cgggataaaa acgttcatcc tcgacgactt cgggatccat caagtcaaga gctgtcaaat
      961 tcggagtcga atagcctaca taatcggcgt tttgcgccgc cacttccggg tcgagcatga
     1021 agttgatgaa cgcatgtgca ccctcaatgt ttttcgcggt tttcggaatg acgatattgt
     1081 cgaaccataa attcgatcct tcctctggaa cgacgtaatc gatatcttca ttttcataca
     1141 tcatatcggc tgcctgtcct gaccatgtca gtgagacggc tgcttcatta ttgatcatga
     1201 gctgtgtaac ttcatccccg atgaccgctt tcacattggg gcttaacgtc tttaatttgt
     1261 ccgttgcttc gcgaagttca tcaaggtcag tcgaatttaa ggaatagccc aacgagttca
     1321 gacccatccc gatcgtttcc ctagcgctat cgacaagaat gacacgttgt ttcaaggaag
     1381 gatcccatag atcatcccag ctcttgaacg tttggccttc gagcaatgtc ggattaaatg
     1441 caatcccaac tgtaccccag aagtagggga ctgagtattg attgccggga tcgaaagcca
     1501 aatccaaaaa gtaaggatcg atatgcttga tattagggat tttactgtag tcgatcggca
     1561 acagaagatt cttttccttc atcatttcaa tcatgtattc ggatgggacg gaaatatcgt
     1621 atgctgtgcc gccttgttcg attttgctca tcatgccttc gttcgaatcg aaagtttcat
     1681 agatgacttt gatgcctgtt tcctcttcga attgcttgat caggtcggga tcgatatatt
     1741 ctccccagtt atagaccgtt atcgtccctt tgccagattt gcttccttca ctcaattcgg
     1801 cattaatgaa aagcaatatg ccggatacga gaagaatcag gatggctgat tggataagtg
     1861 atttcatttc ttcacccccg ctagaggcgt cttcgatttc cggctaatcg catagtaacc
     1921 gagcactaaa gcgactgtga tgataaagat gagtccggaa agtgcgttaa tcgttaagct
     1981 gatgcctgtt cttgccatcg aatagatttc aactgatagc gtcgagaagc cattgcccgt
     2041 tacgaagaac gtgacggcga aatcgtcaag cgaatacgtc agagcgagga agaatccagc
     2101 aaatatccca ggtttaatga atggaaggat caccctcgtc atgacatcct tccgtgatgc
     2161 cccaagatct aatgcagcat cgatcagtga agtgttcatc tccgctagtt ttggcaagac
     2221 catgataacg acgatcggta tgctgaacgc aatatgggaa atcagtacag aagcgaatcc
     2281 gagcttcacc ccgatcatcg taaacaagat aaggaatgac gccccgataa tgacatccgg
     2341 gctgacaatc aaaatattat tcagagaaag tacagcattc ctcattttct tattgcgcat
     2401 gaagtagatc gccatagccc cgatcacgcc aattgctgtt gataatagtg cggaaagcag
     2461 ggcgatgatg attgtattga ggacgatgat gatcaaccgc ttgtcctcaa atactgccgc
     2521 ataatgctcc catgtaaaat cttcgaagtt ggacatcccg ccgcccgagt tgaaagagta
     2581 gaaaatcaag tagaagatcg gggcgtacag gataatgaag atgattgcca aatacagttt
     2641 cggaagctta cctagctttt ccattgtctg tagccccctt ttctttaccg ttcgtgacaa
     2701 gcatgataat gaacatgaag acgatgagga agacggcgat tgtcgagccc attccccagt
     2761 tttgcgtcac taaaaactgc tgttctatgg ccgttccgag cgtaatgact ttgttcccgg
     2821 caatgagcct tgtaatcatg aacaaagata acgcaggaat gaatacgact tggatacctg
     2881 atttcacacc gtttgtcgtc aatggccata tgaccctcct gaaagttgtc caggaatttg
     2941 caccaagatc atgcgatgca tcgatcaatg cagggttcaa tttgtcgagt gcattgaata
     3001 tcggcaaaat catgaacggg atgaaaatgt agactgagac gaatacgaaa ctaaagtccg
     3061 taaacaggat ttgctgcttg ccaatgccaa cggtctccaa aaatgcattc aacggcccat
     3121 ataaacccat caacccgatg aaagcgtatg tctt
//
LOCUS       DASULI010000137         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_366099, whole
            genome shotgun sequence.
ACCESSION   DASULI010000137 DASULI010000000
VERSION     DASULI010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366099"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /gene="ybaK"
                     /locus_tag="VFX34_03005"
     CDS             complement(<1..466)
                     /gene="ybaK"
                     /locus_tag="VFX34_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX5563905.1"
                     /translation="MAKQKKSVKTNAVRLLENEQVPYKLMEYDINDGQLDGVSVAAKT
                     GQAEESVFKTLVTIAGPRELFVFIVPVAEELDLKKAAKAAGVKKLDMLPLKDLTKETG
                     YVRGGCSAIGMKKKYPTVIDASAEQLEQFVVSAGRPGLQMKLSPLDLARIAEA"
     gene            complement(522..1121)
                     /locus_tag="VFX34_03010"
     CDS             complement(522..1121)
                     /locus_tag="VFX34_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563906.1"
                     /translation="MNAIMTFASAADQMQRYDAVRRRNAETAYHRQADYNGRNQKMLD
                     DLEALLTGKKEPEVVQESREFKKLDAGQVKQIALPLGKTLEETIDAWRDVRREAMSVQ
                     EPTTADHQLAATASAKIMETEARIALQKREHSQMEAETNRAKAEAFKVASMDLPNDLE
                     REVLLEQKRFAKAISAYSIQVEAKRNGFIAEWPNYNKTA"
     gene            complement(1324..3492)
                     /locus_tag="VFX34_03015"
     CDS             complement(1324..3492)
                     /locus_tag="VFX34_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006638741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEX5563907.1"
                     /translation="MSKSVVLAEKPSVARDIARVLGCNKKGNGFLEGQKYIVTWALGH
                     LVTHADPEGYGNEYKEWKLEYLPIIPDPFKLTPIRQTTKQFNAVKAQLRRNDVKDVII
                     ATDAGREGELVARWILEMAKNRKPVKRLWISSVTDKAIKDGFNNLKDGRQYENLFEAA
                     VARAEADWVVGINATRALTVKYNAQLSTGRVQTPTLAMIAERERQIREFKPKSYYGLQ
                     ALTETARFTWSDKAGQTQSFNKEAIEKLLAKLDGVHSGKVQDVKTAPKQQPAPPLFDL
                     TELQKEAHRRWSWSAKETLSTLQNLYERHKAVTYPRTDSKHLTSDMAGTLKERIKAVD
                     IGPYRKSVNVLLRSNSIKPQKGVIDDKRVSDHHAIIPTEETPIYQNLSDKEQRLYDLI
                     VKRFLAVFFGPFRYEQVTAELAVEGEIFKAKGRTVTDEGWKAVYSTEEDEEDTDRLPA
                     FAKGEEVKIRAVVMTEGQTKPPARFNEGTLLAAMENPAQFMAGESKDLIKTLGDTGGL
                     GTVATRADIIEKLFSSFVIEKRGNDIFTTSKGRQLLELVPEDLKSPALTAEWEQSLTK
                     IATGKMKKEAFMKEMVDFSKKTVKEIKTDDKKFKHDNVTGKMCPDCGKPLLEVNGKRG
                     KMLVCQDRECGHRRNVSQLTNARCPVCKKKLELRGEGDGRIFVCKCGHREKLSAFEKR
                     RSQSGGKKADKRDVQKYLKKQEEEPVNTALADALKKMFDK"
     gene            complement(3724..4287)
                     /locus_tag="VFX34_03020"
     CDS             complement(3724..4287)
                     /locus_tag="VFX34_03020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5563908.1"
                     /translation="MKVLFVDGTIIGSKTGAMLDTVEEYVREASGDLQIERMNLAEYD
                     HQFVDGRPSEQYNEDMQEMVRKFEEADAYVIATPIFQGSIPGVLKNAFDMLHPHAMRY
                     KPVSIVANGGTYQHHLVPEFQLKPILDYFRCLVTPNFVYTHSGHFDQDNRIIDEDVHN
                     RLRELARVFAKYTEMSQDLSKDALDTN"
BASE COUNT          934 a         1312 c          957 g         1230 t
ORIGIN      
        1 aagcttcagc gatacgggca aggtccagcg gagacaattt catctgcaac ccgggcctcc
       61 cagcactgac gacgaactgc tccagctgtt cagcggaagc atcgatcacg gtcggatatt
      121 ttttcttcat gccgatcgcc gaacagccgc cacggacata cccggtctct ttcgtcaaat
      181 cctttaacgg cagcatatcg agcttcttca cacccgctgc cttcgccgcc ttctttaaat
      241 cgagctcctc cgcgaccggg acgatgaaga cgaaaagctc acgcggaccg gcgattgtca
      301 ctaacgtctt gaagactgat tcctccgcct gccccgtctt tgcagcgacc gacacgccat
      361 ccaactgtcc gtcattgata tcatattcca tcagcttata cggtacttgt tcattttcca
      421 atagccgcac tgcgtttgtt tttaccgatt tcttctgctt cgccatacgt tcacaccttc
      481 ctccaatcaa agaaaggccg ccaaatcggc gacccttcct atcaggctgt tttattataa
      541 ttcggccatt cggcaataaa cccgttgcgc ttcgcttcca cttggatcga gtaagcggaa
      601 atcgccttcg caaaacgttt ttgttccaac agcacttccc gctctaaatc gttcggtagg
      661 tccatggatg ccactttgaa cgcttccgcc ttcgcacggt tcgtttccgc ttccatttgt
      721 gagtgctcac gcttctggag ggctatcctc gcttcggtct ccatgatttt cgcagaagcg
      781 gttgccgcaa gctggtgatc tgcggttgtc ggttcctgaa ccgacatcgc ttccctgcgg
      841 acgtcccgcc acgcatcgat cgtctcctcc aacgttttcc caagaggaag ggcgatctgc
      901 ttcacttgcc cggcatccag cttcttgaac tcccggcttt cttgtacgac ttccggctct
      961 ttcttccctg tcagcaacgc ttccaagtca tcgagcatct tctggttccg cccattgtaa
     1021 tccgcctggc gatgataggc cgtttccgca ttcctgcgtc gcactgcgtc ataccgctgc
     1081 atttggtcgg cagcggatgc gaacgtcatg atggcattca tgtcactcac ctcccgatac
     1141 gtgtccactt tcagttatga gtattatagc catttattgc ttgctgaaac atccagcgaa
     1201 agattcaaag actgattatt cgaaaggtat ttccgaccgg ttgtttatgc ctactttttc
     1261 gacattttat ttcttttatt gcacattact tacagagcat ttatcgatta atcccgctaa
     1321 tcgtcactta tcgaacatct ttttcaatgc atccgccaat gccgtgttca caggctcttc
     1381 ctcctgcttt ttcaaatact tctggacgtc acgtttatcc gccttcttgc ctccggattg
     1441 agaacggcgt ttttcaaatg cggataactt ttcacgatgg ccgcatttgc agacgaagat
     1501 tcgtccgtcc ccttctccgc gcagttcaag cttcttctta cagaccgggc agcgcgcatt
     1561 cgtcaactgg gagacgttgc ggcgatggcc gcattccctg tcctggcaga cgagcatctt
     1621 gccgcgtttg ccattcactt ctagcaaagg cttcccgcaa tccgggcaca tcttccctgt
     1681 cacattatca tgcttgaatt tcttatcgtc cgtcttgatt tccttgacgg tcttcttcga
     1741 gaagtccacc atctctttca tgaacgcctc ttttttcatt ttgccggtgg cgatcttcgt
     1801 caggctctgc tcccattcag ctgtcagtgc gggagatttc aaatcttccg gaacgagttc
     1861 aagcagctgt ctgcccttcg atgtcgtaaa gatgtcattc cctctctttt cgatgacgaa
     1921 cgagctgaat aatttttcta tgatgtctgc gcgggtcgcc accgtgccaa ggccaccagt
     1981 gtcgccgagc gtcttgatca gatcctttga ttcgcccgcc atgaactgcg cagggttttc
     2041 catcgccgca agcaatgtcc cttcattgaa acgcgccggc ggttttgtct gcccttccgt
     2101 catgacgacg gcgcggatct tcacttcttc acctttcgca aaagccggca gcctgtctgt
     2161 atcttcttca tcttcctcag tggaataaac cgccttccac ccttcgtccg tgacggtcct
     2221 gcctttcgcc ttgaagattt ctccttcaac agcaagctcc gccgtcactt gctcatagcg
     2281 gaacggaccg aaaaacacag ctaggaaccg tttgacgatc aaatcataca gacgctgctc
     2341 cttgtcggac aagttctgat aaatcggcgt ctcctctgtc gggatgatcg catggtgatc
     2401 ggatacgcgc ttatcgtcaa tgacgccttt ctgcggtttg atcgaattcg agcgcagcaa
     2461 tacattgacc gatttacggt acggtccgat atcgaccgcc ttgatgcgtt ccttcagcgt
     2521 ccccgccata tcagacgtca aatgtttcga atcggtacgc gggtacgtca ccgctttatg
     2581 ccgctcgtac aggttctgca gcgtcgacaa cgtctctttc gcggaccatg accagcgtcg
     2641 atgtgcttcc ttctgcaatt ccgtcaaatc gaataaagga ggagccggct gctgctttgg
     2701 tgccgttttc acgtcctgca cctttccgga atgaaccccg tcaagtttcg ctaatagttt
     2761 ttcaattgct tctttattga acgactgcgt ctgccccgct ttatcggacc acgtgaacct
     2821 cgcagtttcc gtcaacgcct gcaatccata atacgatttc ggcttgaatt cgcggatctg
     2881 cctttcccgc tccgcaatca tggcaagtgt cggcgtctgg acacgccctg tcgacaattg
     2941 cgcattgtat ttcaccgtca aagcgcgcgt cgcattgatg ccgacgaccc agtccgcttc
     3001 tgcacgcgcg accgccgctt caaacaagtt ttcatactgg cggccatcct tcaagttatt
     3061 gaagccgtcc ttgatcgctt tatcggtgac ggatgaaatc cagagccgct tgactggctt
     3121 gcggttcttc gccatctcca atatccagcg ggcgacaagc tccccttcac gcccagcgtc
     3181 cgtcgcaatg atgacatcct tcacatcatt ccggcgaagc tgcgccttga ccgcattgaa
     3241 ctgcttcgtc gtctgtcgga tcggcgtcaa cttgaacgga tccggaatga tcggcaagta
     3301 ttccagcttc cactctttgt attcattgcc gtacccttca ggatcggcgt gtgtcacgag
     3361 gtgtccgagc gcccaagtga cgatatattt ttggccctcc aagaagccat tgcctttttt
     3421 attacagccg agcacccgcg ctatatctct cgcgacggat ggcttttcgg ccaacactac
     3481 tgatttggac atgaataaat cccctttcta catgttttca ttataaggga gaaagcgttc
     3541 cgttgcattc ccaacgaaat ctcttcgaaa actgaacacc ccgtcagaac cgcctaaagt
     3601 cggaacacaa ccgcctaata cctcacctga accgcctaat gacggatgca caagccgtcc
     3661 atcacaaaaa aagctgtcgc agaaagtcga tttccgactt tccgtgacag cccttcaata
     3721 tgttcaattc gtgtccaatg catccttcga aagatcttgg ctcatctctg tatacttcgc
     3781 aaacactcgt gccaattcgc gcagacggtt atgcacatct tcgtcaatga tgcggttatc
     3841 ctggtcgaaa tggcctgaat gggtatagac aaaattcggt gtgacgagac agcggaagta
     3901 atccaaaatc ggcttcaact gaaattcagg aaccaggtga tgctggtatg taccgccatt
     3961 cgcgacaatc gaaaccggct tgtagcgcat cgcatgcgga tgcagcatat cgaatgcgtt
     4021 tttcaatacg cccgggatgg agccttggaa aatcggagtc gcaatgacat acgcatccgc
     4081 ctcttcaaac ttccgtacca tttcctgcat gtcctcgtta tattgttccg acggacgccc
     4141 atcaacgaat tgatgatcat attccgccag attcatccgt tcaatttgga gatctccact
     4201 agcttctctt acatattctt cgaccgtatc aagcatggcg cccgttttgc tgccgataat
     4261 ggtcccgtcc acaaacaaaa ctttcactgg tcatcctcct caacgctgtt tatacaaaac
     4321 aatcgtacca taatgattgg tacgattgcc ttaaaacggc tctatttgga gatcccattt
     4381 gaccttggag cttctccggg gatgtcacgc ttgcctgccg aagaatcttc ggt
//
LOCUS       DASULI010000138         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_147841, whole
            genome shotgun sequence.
ACCESSION   DASULI010000138 DASULI010000000
VERSION     DASULI010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147841"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VFX34_03025"
     CDS             complement(<1..1315)
                     /locus_tag="VFX34_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5563909.1"
                     /translation="MFMTEVLGEYAKHQPEKVFTSYNGREWTYGEFYGKAKKIAGHFQ
                     AEGYEKGDIIALYALNSDHFLVCYFGIQLGGFIVMPVNTKLAPPEVEYIFNHSEAKVL
                     IYDKRLEDVVNQSKHSFKEKLSIQEDIERLLADNEDLYEPVNMNGEDTAVVMYTSGTT
                     GKPKGVMLTHQNIISTAEIWSEAMSITDADRMLISTPLFHCAACHVFMIPVTYKGGTV
                     IIEEAFSPKGTLDLLQKTSPTMFFGVPAMYTIILNLPEIKNLELPSLRLFGYGAAPMP
                     YEILKKLKETFPKVKVQNLYGQTENSPAASTLKDHLALDKIGSVGEALPQTEVRVVDE
                     FGEPLPTGQVGEIVMRGPQVMKGYLKDEEETRRTIKNGWLYTGDLGRMDEDGLLYIVD
                     RKKDMIIRGGENVYPVEVEEVLYQIPGLFEAAVVGVPHEVLGEVPK"
     gene            1491..2429
                     /locus_tag="VFX34_03030"
     CDS             1491..2429
                     /locus_tag="VFX34_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX5563910.1"
                     /translation="MAEDRKQSYRNWLVWAGGSVCLLLIGVICYALFFVNELAETAKE
                     IHEPMERVFSEKREVPITFKEKDPFSVLILGVDEREGDKGRSDTMIVLTVNPMLQSTK
                     MVSIPRDTYSEIVGMGFEDKLNHAYAYGGIDMSLVTVEHFLDIPIDYVVQVNMESFKE
                     IIDAIGGVSVTNTLDFSVDGHSFPEGELILDGEEALVFVRMRMDDPRGDFGRQDRQKQ
                     VIQGVLQKGAKLSSLLKFKSIFDAIGNNVRTNMTFDEMVEVQKNYRDAAKSVEQIKFE
                     KGEGKRMEGIWYYMMDSEELEVVKAELKAHLEMELR"
     gene            2742..>3101
                     /locus_tag="VFX34_03035"
     CDS             2742..>3101
                     /locus_tag="VFX34_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563911.1"
                     /translation="MEDGNLLVYGLKLGDSPSTAEEIWGVPEILEEENTIHGETYHYY
                     PSMDMTVGYYEEKLSTLAVDADEEDLQEITDKFQGDHYRTPEGDAEFFFDPETGQLLI
                     YGTANSVDGNAELRLLMS"
BASE COUNT          811 a          695 c          768 g          827 t
ORIGIN      
        1 cttttggcac ctcccccaac acttcatgcg ggacaccgac gacggccgct tcaaacagcc
       61 ccggaatttg ataaagcact tcttcgactt ccaccgggta gacattttca ccgccgcgga
      121 tgatcatatc tttcttccgg tcgacgatat aaagcagacc gtcttcgtcc attcgtccga
      181 gatcgcccgt ataaagccag ccattcttga tcgtccgcct tgtttcctct tcatctttca
      241 aatagccttt catcacttgc gggccgcgca tgacaatttc ccctacttgg ccggtcggaa
      301 gcggttcgcc gaactcatcg acgacccgga cttctgtctg cggcaaggct tcaccgacag
      361 agccgatttt atcgagtgcc aggtgatctt taagtgtcga agcagccggt gagttttccg
      421 tctgtccata tagattttga actttcactt tcgggaatgt ctctttcagc ttcttcaata
      481 tttcatatgg catcggggca gctccgtaac cgaacaggcg aagggatggc agctcaagat
      541 tctttatttc cggcaagttc aaaatgatgg tatacattgc cggaacaccg aaaaacatcg
      601 ttggcgatgt tttttgcaat aagtccaatg tccccttcgg agagaatgcc tcctcgatga
      661 tgaccgttcc gcctttatac gtgacgggaa tcatgaagac atggcaggct gcgcaatgga
      721 agagcggagt tgagatgagc atcctgtccg catccgtaat cgacatcgcc tccgaccaga
      781 tttccgcggt cgatatgata ttttgatgcg tcagcatgac ccctttcggc ttgcccgtcg
      841 tcccggatgt atacatgacg actgccgtat cttccccatt catatttacg ggttcataaa
      901 gatcctcatt gtctgcaagc aatctttcaa tatcctcttg tatcgacagt ttttctttga
      961 atgaatgctt cgattgattg acgacgtctt ccaatctttt atcataaata agcactttcg
     1021 cttctgaatg gttgaagata tattccactt ccggcggtgc caatttcgta ttgaccggca
     1081 tgacaatgaa gccgcccaac tgtatgccga agtagcagac gagaaagtgg tcggagttca
     1141 gcgcataaag ggcgatgata tccccttttt catacccctc cgcttggaaa tgccccgcga
     1201 tctttttcgc tttcccataa aactcaccat acgtccattc cctgccgtta tatgaagtga
     1261 acactttttc cggctgatgt tttgcatact cccccaacac ttctgtcatg aacatggaca
     1321 atcatcccct ttgcaccaat ttatcatcta cgtatattct agcaaccgat atttcagatt
     1381 acaagttata taattaatat tcagataatg ataaaagtgt ctatttcttc tactatctgc
     1441 gtaaactagt aagcatcatt tttattctga atgactggcg ggagagaaga atggcagaag
     1501 accggaaaca atcgtatagg aattggttgg tatgggcagg cggttcagtt tgcctcctat
     1561 tgatcggcgt catttgctac gcgctgtttt ttgtcaatga actggcggaa acggcgaaag
     1621 agatccatga gccgatggag cgcgtatttt cggaaaaaag ggaggtcccg atcacattta
     1681 aagagaaaga tcccttttcc gtactgattt taggagtgga tgaaagggag ggcgacaaag
     1741 gccggtccga tacgatgatt gtgctaacag tgaatccgat gcttcaatcg acgaaaatgg
     1801 tgagcatccc gcgcgatacg tacagcgaaa ttgtcgggat gggctttgag gataaactga
     1861 atcacgctta tgcttatgga ggtatcgata tgtcgcttgt cactgtggag cattttctcg
     1921 acataccgat cgactatgtc gtgcaagtga atatggaaag cttcaaggaa atcattgatg
     1981 caatcggggg tgtctccgtt acgaatacac ttgatttttc tgtcgatggg cattcatttc
     2041 cggaaggtga actgattttg gacggtgagg aagcactcgt ctttgtccgg atgcgcatgg
     2101 atgacccgcg cggcgatttc gggcggcaag acaggcagaa gcaagtcatt caaggggtgt
     2161 tgcaaaaagg cgcaaagctt tcgagtttat tgaaattcaa aagcatcttt gacgcaatcg
     2221 gcaataatgt acggacgaat atgacgttcg acgaaatggt cgaagtgcaa aaaaattacc
     2281 gggacgccgc taagtccgtt gagcaaatca agtttgagaa gggtgaaggg aaacggatgg
     2341 aaggaatctg gtattatatg atggattccg aagaactcga agtagtgaag gctgagttga
     2401 aggcacattt ggaaatggaa ttacgttgat gcagcccatt gcagaggggc tgtttttttg
     2461 ctttcatttg ataagcaggc ggatgtgttg gataatcttg ttagcgggtg aactcaatta
     2521 tatctaacgg gaggatgatt tcgaatgaaa cgctcacttt ggttattgat tgcagtttca
     2581 attttactag tagcgggatg ttcaaaagat aagctgagca gaagccggtc gaggcggaag
     2641 agacgaacgc atctgataat ggggagctcg acgaacagga cccgacggat gaaggcgctg
     2701 accaaacgaa tgaggcggaa caaaaaccgc cgctctttga tatggaagat ggcaatctgc
     2761 tcgtttatgg cttgaaattg ggggactccc cttcaaccgc ggaagagata tggggcgttc
     2821 cggaaatctt ggaggaagag aacacgattc acggagaaac ctaccattac tatcctagca
     2881 tggacatgac ggtcggttac tatgaagaaa aactttcaac ccttgctgtg gacgcggatg
     2941 aagaagacct tcaggaaatc accgacaagt tccaagggga ccattatcgg actccggaag
     3001 gggatgccga attcttcttc gatccggaaa ccggacaatt gctcatttat gggactgcaa
     3061 acagtgttga cggcaatgcg gaattgcggt tgctcatgtc c
//
LOCUS       DASULI010000139         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_817999, whole
            genome shotgun sequence.
ACCESSION   DASULI010000139 DASULI010000000
VERSION     DASULI010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817999"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFX34_03040"
     CDS             <1..813
                     /locus_tag="VFX34_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX5563912.1"
                     /translation="RLLFKEEALVAICGGGLAPTIEGLPVPMWRPVFIRKGSELLFGA
                     AKAGSRAYLAVAGGLDVPIVLGSRSTYVKAGLGGVEGRALKKGDVLHIGPPAMLSKVI
                     TEELASGRTFQSADWQIAPKLLPDLSNHYEIRVMRGRQYELFDEASRHRFWNELFTVS
                     PQSDRMGYRISGPALQLKEPMELISEAVTYGSIQVPGDGNPIILAADRQTTGGYPKIG
                     QLSAIDFTKLAQAKAGDRLSFKEVTIEESQRLVARREMSLKKLHAAIRVKFR"
     gene            824..1594
                     /locus_tag="VFX34_03045"
     CDS             824..1594
                     /locus_tag="VFX34_03045"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEX5563913.1"
                     /translation="MGYVVDLNCDMGEGFGAYVMGNDKALMNTITSANIACGFHAGDP
                     TTMRRTVRMAHEKNVGIGVHPGLQDLIGFGRREMAISAEEAFNLVVYQIGALSAFVKA
                     EGGRLQHVKPHGALYNMAAVNRDLAEGIAKAVYKVDSELILFGLAGSELVKAGEQIGL
                     KVAHEVFADRTYQADGTLTSRREPNALITDHEVAIRQIVRMVKEGKVLATDGTDVEIK
                     ADTICIHGDGKTAVEFARVIPGALREKGIEVRKIGDFL"
     gene            1618..2082
                     /locus_tag="VFX34_03050"
     CDS             1618..2082
                     /locus_tag="VFX34_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5563914.1"
                     /translation="MTVIFVTGLSGVGTSSALEQLKKEGYNAVDTDYGYIKVIKNGGN
                     EERLLDEEKITQLLEDNKQSHLIISGCYANQGKFYKHFNYVVLLKADLNVMLDRINNR
                     TSNNYGKSPEERQEVIGSFENVLPLLEESSDIIIDTTHHGIAEVCRQLKELL"
     gene            complement(2255..2974)
                     /locus_tag="VFX34_03055"
     CDS             complement(2255..2974)
                     /locus_tag="VFX34_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388566.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5563915.1"
                     /translation="MGRKWNNIKEKKASKDANTSRIYAKFGRELYVAAKQGEPDPELN
                     QNLKMVIERAKTYNVPRAIIDRAIEKAKGGAEENYDELRYEGFGPNGSMVIVDALTNN
                     VNRTASEVRAAFGKNGGNMGVSGSVAYMFDATAVFGLEGKTADEVLELLMEADVEVRD
                     ILDEEDTVIVYAEPDQFHAVQEALKNAGVTEFSVAELTMLAHNDVELDAEAQAQFEKM
                     IDALDDLEDVQQVYHNVDLGE"
     gene            complement(3117..3821)
                     /locus_tag="VFX34_03060"
     CDS             complement(3117..3821)
                     /locus_tag="VFX34_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5563916.1"
                     /translation="MTANGHCRHGDGTAEMHKMCISIVPIFNHLDPSEMNEIVKHTHS
                     ISHPRGHTIYHAGNISDGLYIVHKGRVKIYRLSENGKEQLVRILGPGDFTGELSLFSE
                     TEHDAYAEAMQNVELCVMSRDNFQMFLLKYPAISLKVLGEFANRLANTEKQAARIALE
                     TTDTRIAMYLADLAEDRNSSRIQLPMTRKDLASHIGTSPETISRKLAEFEDAGWIRQL
                     SQREIEILDMDALLLV"
     gene            complement(3835..>4437)
                     /locus_tag="VFX34_03065"
     CDS             complement(3835..>4437)
                     /locus_tag="VFX34_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5563917.1"
                     /translation="VLSIADRIRPEAADALRELRTSGVKQIIMLTGDNRHTAELVGVQ
                     LGLDQVHAELLPEDKVAIIQQLKDAGHRVAMAGDGINDAPAIATADIGLAMGEGGTDI
                     SMETADVVLMADRLDQFSHAYSLAKATVRNMKQNTFFAVGTVALLLVGVLLGKVFLAS
                     GMLIHELSVLLVILNAIRLIRYTKRKAEKRKNLEPDLLPG"
BASE COUNT         1129 a          980 c         1151 g         1177 t
ORIGIN      
        1 cggctattat tcaaggaaga ggcattggtc gcgatttgtg gcggaggttt ggctccgaca
       61 attgaaggct tgcctgtacc gatgtggaga ccggtattca ttcggaaggg aagtgaattg
      121 ctgttcgggg cggccaaggc ggggagcaga gcatatttag cagttgcggg agggcttgat
      181 gtaccgattg tgctcggaag ccggtcgacg tatgtaaagg cggggcttgg cggggtggaa
      241 ggaagggcat tgaagaaggg ggatgtcctt catattggtc cgccagccat gctttcaaag
      301 gtcataacgg aagagcttgc atctggcagg acgtttcagt cggcagattg gcaaattgca
      361 ccgaagcttc ttcccgatct atcgaaccac tatgaaattc gggtcatgcg cggcaggcag
      421 tatgagttat ttgatgaagc gagtcgacat cgtttttgga atgaactctt caccgtcagc
      481 ccgcagtcgg atcggatggg ttaccggatc agtggtcccg ctctgcagct gaaggaacct
      541 atggagctga tttcggaagc agttacttat ggatccatcc aagtgccggg ggatgggaat
      601 ccaatcattc ttgcagcgga tcggcagacg acgggcggtt atccgaaaat tggacagctt
      661 tccgcgattg attttacaaa acttgcgcag gcgaaggcgg gggaccggct ttcattcaaa
      721 gaagtgacaa ttgaagaatc gcaacgactc gttgcaaggc gggaaatgag tttgaaaaag
      781 ttgcatgcgg caatacgggt taaattcaga taggagtggc ggaatgggat atgtagtcga
      841 tttgaattgt gatatgggcg agggcttcgg tgcatatgta atggggaatg ataaggcgct
      901 aatgaacacg attacgtcag cgaatattgc atgcggcttc catgcaggcg atccgacgac
      961 gatgcggagg acggtgcgga tggcgcatga gaaaaacgtc ggaatcggcg tgcacccggg
     1021 tttacaggat ttgattggtt tcggtcgacg tgaaatggcg atttcagcag aagaggcatt
     1081 caatctcgtc gtatatcaaa tcggtgcatt gtccgcattt gtaaaggcgg agggcggacg
     1141 cttgcagcat gtgaagccgc atggcgcgct ttataatatg gccgccgtga accgggactt
     1201 ggccgaggga atcgcgaagg ctgtctataa agtcgattcg gaattgattc tattcggact
     1261 tgctggcagc gagctagtca aggctgggga gcagatcggg ttgaaggtgg cgcatgaagt
     1321 gttcgcggat cggacttatc aagcagacgg gacattgaca tcaagacggg agccgaacgc
     1381 attgatcacc gaccacgaag ttgccatccg ccagattgtc cggatggtga aggaagggaa
     1441 agtgcttgcg accgatggaa cggatgttga aatcaaagcc gatacgattt gcattcatgg
     1501 agatgggaag acggcggtgg aattcgcgag ggtgatacct ggggctttgc gtgagaaagg
     1561 gattgaggta aggaaaatcg gcgacttttt atgaatgaga gtcgaaaggg ggataacatg
     1621 acagtcatat ttgttacagg cttatcgggg gtaggtactt caagtgcatt agaacaatta
     1681 aagaaagaag gatacaatgc agtcgatacg gattatggct acataaaagt aatcaaaaac
     1741 gggggaaatg aagaaagact cttagatgaa gaaaaaataa cacaattact tgaggacaat
     1801 aaacaatctc atttaattat ttcaggctgc tacgctaatc aagggaagtt ttataaacac
     1861 tttaattatg tagttctact aaaggctgat ctgaacgtga tgcttgatag aataaataac
     1921 agaacttcta ataattatgg taagagcccg gaagagagac aagaggttat aggtagtttt
     1981 gaaaacgtat tgccgttatt agaagagagt tctgacatta ttattgacac tacacatcat
     2041 ggaattgccg aagtttgtag acaactaaaa gaattattat aaatactagt gtcttttatg
     2101 aacgaaaggg agctttagtt aaagaagaca ataatatata taaaagaact tccgaaaatc
     2161 ggtagttctt tttatttctc tctatcatga actaacttta acagagccat tcagataaag
     2221 aaaaaaccgc cagtcacggc ggtttttaca aggtttattc tcctaaatca acattatgat
     2281 acacttgttg aacatcttcc aagtcatcca aagcgtcgat cattttctca aattgtgctt
     2341 gagcctcagc gtccaactct acgtcattat gcgcgagcat tgtcaattcc gctacggaga
     2401 attccgtgac accggcgttt ttcaacgctt cttgcaccgc gtggaattga tccggttccg
     2461 cgtagacgat gactgtgtct tcttcatcta aaatatcacg gacttcgacg tctgcttcca
     2521 ttaatagttc caacacttca tccgcagttt taccttctag gccgaagacg gcagtcgcat
     2581 cgaacatgta cgcaacggat ccgctgacgc ccatgtttcc gccgtttttc ccgaatgctg
     2641 cgcgcacttc ggaagcggta cgattgacgt tatttgtcag cgcatcgacg atgaccatcg
     2701 atccgttcgg gccgaagcct tcgtaacgaa gctcgtcata gttttcctcg gcgccgcctt
     2761 tcgccttctc gattgcacgg tcaatgattg ctcttggcac attgtatgtt ttcgcccgtt
     2821 caatgaccat tttgaggttc tggttcaact cgggatcggg ttcaccctgc tttgctgcta
     2881 catataattc acgcccgaac tttgcgtaaa tacggcttgt attcgcatcc ttcgaagctt
     2941 ttttctcttt aatattattc catttccggc ccatcttttt cacactctct ttcactctga
     3001 gcatagattt catcgagcta ttatacaccc gttgcatgaa tgctgacaag cgaaaaatcc
     3061 tttaaatgga atggcgtgag ttctcgttaa tccccggcaa gttctcgttc ctacacttaa
     3121 acaagcagca acgcatccat atccaaaatt tcgatctccc gctggctgag ctgacggatc
     3181 caccctgcgt cctcgaattc cgcaagtttc cggctaattg tttcgggcga tgtcccgata
     3241 tgcgatgcca agtcctttcg cgtcatcggc agttggatcc ggctcgaatt ccggtcttca
     3301 gcaagatcgg caaggtacat ggcgatgcgt gtgtctgtcg tttccaaggc gatccgggct
     3361 gcctgttttt ccgtattcgc aagccgattg gcaaattcgc caagcacttt aagtgaaatg
     3421 gcggggtatt tcaataaaaa catttggaaa ttgtcacggc tcatgacaca tagctcaacg
     3481 ttctgcatcg cctccgcata agcatcgtgc tccgtttcgc tgaataatga aagttcacct
     3541 gtgaaatcac cgggtcctag gatcctcacc aattgctcct tgccattttc agaaagccga
     3601 tagattttca cgcggccttt atggacgata tatagtccgt ctgaaatgtt gcctgcatgg
     3661 tagattgtgt gtccccgggg gtggctgatt gaatgggtgt gcttgacgat ttcattcatt
     3721 tccgaagggt cgagatggtt gaaaatcgga acgatagaga tgcacatttt atgcatctct
     3781 gctgtcccat caccgtgccg gcaatgtccg ttggctgtca tcatcgttca ctccttatcc
     3841 cggaagtagg tcaggctcca agtttttccg tttttccgcc tttcttttag tatagcggat
     3901 taaccggatg gcgttcaaaa taacgagcag cacactcaat tcgtgaatca acattccgga
     3961 agcgagaaac acttttccga gcaacacacc cactagaagc aatgcgacag taccgactgc
     4021 gaagaatgta ttctgcttca tattccggac agtcgctttt gcaagtgaat acgcgtgtga
     4081 aaattggtcg agccggtctg ccataagaac aacatcggca gtttccatcg atatgtcggt
     4141 tccgccttcg cccatcgcaa gtccgatatc cgcggtcgcg attgctggtg cgtcgttgat
     4201 gccatcgcct gccatggcga cgcggtgacc ggcatctttc aattgttgga taatggcgac
     4261 tttgtcttcc ggcaaaagct ctgcatggac ttgatccaaa cctaattgta caccgacgag
     4321 ttcagctgta tggcggttgt cgcctgttaa catgatgatc tgtttcacac cgcttgtgcg
     4381 gagttcgcgg agcgcatctg ccgcttcggg acggatcctg tccgcgattg acagaac
//
LOCUS       DASULI010000140         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_916649, whole
            genome shotgun sequence.
ACCESSION   DASULI010000140 DASULI010000000
VERSION     DASULI010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916649"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFX34_03070"
     CDS             complement(<1..327)
                     /locus_tag="VFX34_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563918.1"
                     /translation="MFQSFLSAIKGLFIKEQSDPTSFLDYALQNMDKLDELQQELLSK
                     SSTLGNPFQSANALSSLNIGTNSNSFHSYDDDFHHDFHHDLHDQIQQDFHNQQFMERM
                     HDPYLNL"
     gene            513..1823
                     /locus_tag="VFX34_03075"
     CDS             513..1823
                     /locus_tag="VFX34_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEX5563919.1"
                     /translation="MTRLTDHLLVLSFDCLSSLDIPFIEQLPNFQRLLDNSSYCQSVE
                     TIYPSVTYPCHTTIVTGKFPARHGIVNNTLLQPGRPSPDWYWHRRHIKGTTLYDEAKK
                     AGMTTAALLWPVTAKANIDYHMPEIFANRPWHHQVPVSLMNGNVRYQLEMNKRFGHIR
                     NGLAQPQLDDFVLESTVHTIKTKKPNLMLVHFVDVDAHRHLHGFSSDEAFAGIRRHDT
                     RLGRIFEALEESDMLDKTTIVALGDHSALDESKAVKLNVLLRENGFIEVNKKGRVKTW
                     KAYCKSCDGSAYIYLKDEKDTRTREAVQALLHSLVVNEANGIEWVLTGEEAGKRGADP
                     KAAFMVEARRGFYITEHMEGDYIDDITPEDVKAKKYTVTSHGYSPEKENYTTVFIAKG
                     KGIRPNVSLPAMHLVDEGPTFARLLGLDLGQTDGRVLDEILATE"
     gene            1907..3196
                     /locus_tag="VFX34_03080"
     CDS             1907..3196
                     /locus_tag="VFX34_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020862408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5563920.1"
                     /translation="MKLRYTKEEKSWMYYDWANSAYSIIITTAVFPLFYKSVARNAGV
                     ADAMSTAYLGYTIAIATFILAMLGPILGTIADYEGMKKKFFGFFFTMGLAATVSLAFI
                     PGEQWLPLLIVYVFAAVGFTGANVFYDAFIVDVTNESRMDNVSARGYGLGYIGSTIPF
                     AISIAIILLSGEGMLPFSSTVATKIAFLITAVWWGLFSIPMLKHVHQKHFIQRESNVV
                     RQSFKRLGNTLKDIRQYRGLFLFLLAYFFYIDGVGTIIGMSTAYGSDLKISPESLLII
                     LFVTQVVAAPFAVIYGKLADRFTGKKMLYVGIVVYIIVCIYAFFLKTTLDFWVLAMLV
                     ATSQGGIQALSRSYFGKLVPKEKANEFFGFYNIFGKFAAIMGPLLVGATAQITGNSAY
                     GVFSLVILFIVGLILLIRVPDPDAETSIPRGAGVVSS"
     gene            complement(3349..>4237)
                     /gene="ligD"
                     /locus_tag="VFX34_03085"
     CDS             complement(3349..>4237)
                     /gene="ligD"
                     /locus_tag="VFX34_03085"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5563921.1"
                     /translation="IPEKVEITHPDKPVWPELGLQKDDFLLYLQTAAPHLLPFLKDRL
                     LTVIRFPHGMLGEGFYQKNVPDYVPDFVQTKVDEDIEYIVCNDLETLLWLGNQLALEM
                     HIPFQTINTKNPTEIVFDLDPPSVNDFSLAVEAAVRMKVIFDQFSLPSYIKTSGGKGL
                     QLYIPLEVDAFTYDDTRVFMKFVCNFLIEQEPQLFTTERLKKNRGNKLYLDYVQHHEG
                     KTIVAPYSTRGNEKGLVATPLRWEEVNGSLRPDAFTIPAVVERLRKEGNPFRDFREKA
                     EGQRLRDVIAHIRDLTNET"
BASE COUNT         1128 a          906 c         1027 g         1176 t
ORIGIN      
        1 aagatttagg taggggtcat gcattcgttc catgaattgt tggttatgaa aatcttgttg
       61 tatttgatca tggagatcat gatgaaaatc gtggtggaaa tcatcatcat agctatggaa
      121 tgaattactg tttgtgccaa tattcaagct gctcaacgca ttggctgatt gaaatggatt
      181 tcccagagtg gagctttttg ataagagttc ctgctggagc tcgtcaagct tgtccatatt
      241 ctgcagagca taatctagaa atgaagtagg atcactttgc tcttttatga aaagtccttt
      301 gattgcagag agaaacgatt gaaacatgtc gttgcctcct tccgatatgg cagacctagt
      361 aaagaatcac tattctttgg ataatcatag caatcggata agcaacttac aatgcattcc
      421 ttgcataatt aaaaaaattc aatcacttct ttcgctccac ccattgttcc cgtatactca
      481 aagtactagt tttatagcac aggaggatac aaatgactcg acttacggac cacttgcttg
      541 tcctttcgtt tgactgtctg tcttccttgg acattccgtt cattgaacag ctgccaaact
      601 ttcaacgact gctcgataac agctcctact gccaaagcgt agagacgatt tatccgtccg
      661 ttacctaccc gtgccacacg accattgtga caggcaaatt cccggctcgt catggcatcg
      721 tcaataacac gctgcttcag cctggccgcc cgtcgcctga ctggtattgg caccgtcgcc
      781 atataaaagg aacgacattg tacgacgaag caaaaaaggc aggaatgacg actgcggcgt
      841 tactttggcc tgtcaccgcg aaggcgaaca ttgattacca tatgccggaa atctttgcga
      901 accgaccgtg gcatcaccag gttccagttt cgttgatgaa tggaaatgtt cgctatcagc
      961 ttgaaatgaa caaacggttt ggccacattc ggaatgggtt ggcacagccg caacttgatg
     1021 actttgtgtt ggaatcaacc gtccatacaa ttaaaacaaa aaagccgaat ctgatgctcg
     1081 tacactttgt cgatgtcgac gcacatcgcc atttgcatgg gttctcttca gatgaagcat
     1141 tcgccggtat ccgtcgacat gacacgcggc ttggcagaat atttgaggcg cttgaagaga
     1201 gtgacatgct tgataagaca accattgtcg cgctcggaga ccacagtgct ctggatgaat
     1261 caaaagctgt gaaactcaat gtgttgctcc gcgaaaatgg gttcattgag gtcaacaaaa
     1321 agggcagggt gaagacgtgg aaagcctatt gcaagagctg tgacggctcc gcatatattt
     1381 atctaaagga tgagaaggac acgcggacaa gggaagctgt acaagccctt ctccattcgc
     1441 tcgttgtgaa cgaagccaac gggattgagt gggtgctcac tggggaggag gcagggaaga
     1501 ggggtgcaga tccgaaggcg gcatttatgg ttgaggcacg gagaggcttc tacataacgg
     1561 aacatatgga gggcgactac attgatgaca ttactccaga agacgtgaaa gccaaaaagt
     1621 acacagttac atcccatggg tactccccag agaaggaaaa ctatacaact gtttttattg
     1681 cgaaggggaa aggaattcgg ccaaatgtaa gccttcctgc catgcatctc gttgatgaag
     1741 gaccgacatt tgcaaggttg ctcggccttg atttaggaca gacggatgga cgagttctgg
     1801 atgagattct agcgacggag taagaattat aaaaataatt aaactattag tttggtagtt
     1861 cttgtataat gggagaaagg gagaaatgaa aaagggggga actgctatga agctgcgtta
     1921 tacaaaggaa gaaaaaagct ggatgtatta tgactgggcg aactcagcgt attccatcat
     1981 tattacgacc gctgtctttc cgctgttcta taagtcggtg gcacgaaatg caggggtagc
     2041 ggacgcaatg tcgaccgcgt atttggggta caccattgcg attgcgacat ttatattagc
     2101 gatgctggga ccgattttag ggacgattgc ggattacgaa ggaatgaaga aaaagttctt
     2161 cggatttttc tttacaatgg gattggctgc gacggtatca cttgccttta ttccaggtga
     2221 acaatggcta ccgttgctca ttgtgtatgt gtttgcggca gtcggattta cgggggccaa
     2281 tgtgttctat gacgcgttta ttgttgatgt cacgaatgaa agccgaatgg acaatgtatc
     2341 ggctcgtggc tatgggctcg gctatatcgg aagtacaatt ccgtttgcca tcagtatcgc
     2401 aatcatttta ttgtcgggag aagggatgct gccgttttcg tcaactgttg cgactaagat
     2461 tgccttcttg attacggcgg tctggtgggg gttgttcagt attccgatgc tgaagcatgt
     2521 tcatcaaaag cactttatac aacgtgaatc aaacgttgtc cgacaaagct ttaagcgcct
     2581 tgggaatacc ctaaaagata ttcgccaata ccggggattg ttcttattct tactcgcata
     2641 cttcttctat attgatgggg tcggcacaat cattggaatg tctactgcgt atgggtcaga
     2701 cctaaaaata agcccggaaa gcctgctgat cattttattc gtaacgcaag tcgttgcggc
     2761 accgtttgcc gtgatttatg ggaaattggc ggatcgcttc actggtaaga aaatgcttta
     2821 tgttggaatc gtcgtttata ttatcgtttg tatttatgcg tttttcctaa aaacgacgct
     2881 ggatttctgg gttcttgcca tgctcgtcgc gacgtcccag ggaggaatcc aggcattgag
     2941 ccgatcctat ttcgggaaac tcgtgccaaa agagaaggca aatgaattct tcggtttcta
     3001 caatattttc gggaagtttg cagccatcat ggggccgctc ttggtcgggg cgactgcaca
     3061 gattacaggg aactctgcat acggggtatt cagcttagtc atcctgttca tcgttgggct
     3121 cattctctta attcgtgttc cggatcctga tgcagaaaca agcattcctc gcggagcagg
     3181 agttgtatct tcataacgaa aaaaaagcag tcaccgatgt gactgctttt ttgtatgctt
     3241 cggcttgagg attccaacgt ttcacgaaag gattacaacg ctcggcctta ggattccaac
     3301 gtttggcttg aggattccaa cgctcgaatt ctcaccttta aaaaattctc atgtttcatt
     3361 tgtgaggtct cgtatatgcg caatgacatc acggagtctt tgtccctctg ctttctcccg
     3421 gaagtcacga aacggattgc cttccttgcg caatcgttct actacagcag ggatggtaaa
     3481 ggcatccggc cgaagcgatc cgttgacttc ctcccaacgc aatggcgttg cgaccaatcc
     3541 tttttcgttt cctctcgtgg aatagggggc cacgattgtc ttgccttcat gatgttgcac
     3601 gtaatccaga tagagcttgt tgccacggtt tttcttcaac ctctctgtcg taaacaactg
     3661 tggctcctgc tcaattaaga aattacaaac gaacttcatg aacactcgtg tatcgtcgta
     3721 ggtaaaggcg tcgacttcta acggaatgta gagctgcaat ccttttccgc ctgacgtttt
     3781 aatataggaa ggcaacgaaa attgatcaaa aatcactttc attcgaaccg ctgcctcaac
     3841 tgctaatgaa aaatcattaa ctgaaggtgg gtctaggtca aacacaattt ctgtcggatt
     3901 tttcgtgttg attgtctgaa acggaatgtg catctcgagt gcgagctgat ttccaagcca
     3961 aagtaacgtc tccaaatcat tgcacacgat atattcaatg tcttcgtcaa cttttgtttg
     4021 aacaaaatca ggtacataat cgggaacgtt tttttggtaa aagccctcgc ccaacattcc
     4081 gtgcgggaaa cggatcaccg ttagcaaccg gtcttttaaa aaaggaagca aatggggggc
     4141 ggcggtttgc aaatacaaaa gaaagtcatc tttctgaagc ccaagctctg gccatacagg
     4201 cttgtcagga tgggtgattt ccaccttttc aggaatc
//
LOCUS       DASULI010000141         3463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_370797, whole
            genome shotgun sequence.
ACCESSION   DASULI010000141 DASULI010000000
VERSION     DASULI010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370797"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..659)
                     /locus_tag="VFX34_03090"
     CDS             complement(114..659)
                     /locus_tag="VFX34_03090"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5563922.1"
                     /translation="MILLEGRTCYLRILTEDDAAIFTELLKANREYWTVFEPRHEKAY
                     YTVSVQRDKIRESIYQMRDRREYNFGIFDAETSRLIGHISLYSIKRLPFSSGFVGYSI
                     DERETGRGLATEAVKIVTKFAFETVALHRVEAFVSPRNAGSVRVLENAGFVREGLLRK
                     LLYINGFWEDHFMYAKLAEDY"
     gene            complement(667..2472)
                     /locus_tag="VFX34_03095"
     CDS             complement(667..2472)
                     /locus_tag="VFX34_03095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017187446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5563923.1"
                     /translation="MTTTKTYSQVWDLEVFFKGGSDSEELRSHLDAVKEQIGALENLA
                     AATQTPTSVEDADQILHVIDEIKNTAKNLREAGAVIGCFIAANTKDKKALLLQAEVGS
                     LGARFQTAILKIKQVVAKTEETVWNELLTTEQLSQFDFVLQEWREEVQLKLSEEEESL
                     ITALSVDGYQAWGQLYDQLVGDISIEMNVDGEEKKLSVGQANNLSSHANIEVRKEAFE
                     KLEEAWESKEDFFASALNHLAGFRLAVYEKRGWDSVLKEPLMRNRMSQETLDAMWGAI
                     SANKAPFAEYLKRKAELLGNENMNWYDLDAPVTSSTKEMPFQEGAEFILEHFGKFGPE
                     LESFSRKAFEDSWIEAEDRADKRPGGFCTGMPVSEQSRIFMTYSGSMSNVATLAHELG
                     HAFHSYVLRPVHYMNRQYAMGVAETASTFAEMIVADASVKAAETEEEKLALLEDKIQR
                     SVAFFMNIHARFLFETRFYEERKNGVVPAARLNELMAEAQKEAYAGALDTVHPHFWAS
                     KLHFYITGVPFYNFPYTFGYLFSLSIYAKAIAEGESFEQKYMALLRDTAVMSVEDLAM
                     KHLGEDITKEEFWAKGVALCVKDVEEFMKIASKER"
     gene            2633..3382
                     /locus_tag="VFX34_03100"
     CDS             2633..3382
                     /locus_tag="VFX34_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEX5563924.1"
                     /translation="MSRILLMTLSLCTVILLNITASFYSLNGNTTGEITDRLPLLFLP
                     ADYVLIICWLLYIMIFFWIYNFKKTCHKMSSEHRNRVTFLFVISCLLHIAWIFLWHFE
                     LFTGTVITKMALLFALLTFYFTFPKQDNQMLGRAPFSLLTGWIFITTISNVSYLLMIH
                     GWSGFGLSDPLWTVIFLTVSTAFALHFMYHHKDYVMNLVFVWAFIGIAVRNGTEELFI
                     SAAAIFLSAVIVTCIFLFSGRRTDNKNRVGA"
BASE COUNT          860 a          867 c          684 g         1052 t
ORIGIN      
        1 accatttgct cgtaacgcta cgcttttact cgcaaaaata aaaagtcgat tcttccttag
       61 aagaatcgac tttttatttt agctatcagt ttccgccagg cggccttgcc ccttcaataa
      121 tcttccgcca atttggcata cataaaatga tcttcccaaa aaccgttaat gtacaatagc
      181 ttcctcagca aaccttcgcg gacaaatccc gcattctcca gcactctcac tgaaccggca
      241 tttcgtggcg atacaaacgc ctccacccga tgcagtgcaa cggtttcgaa tgcaaatttt
      301 gtaacgatct tcaccgcttc agtcgcaagc cccctgcccg tttcccgttc atcgattgaa
      361 tacccgacga agccgcttga gaacggaagc ctttttatgc tgtataacga tatatgaccg
      421 atcaacctgc ttgtttcggc gtcaaaaatc ccgaaattgt attcgcgccg atctctcatt
      481 tgataaatgg attcccttat cttatcccgt tggacagaaa ccgtatagta ggccttttca
      541 tgacgaggct caaagaccgt ccaatattcc cggttcgctt tgagcagctc ggtgaaaatc
      601 gcggcgtcgt cttctgtcaa tatacgcaaa taacaagttc gcccttcaag cagaatcaat
      661 gcttcttcac ctttctttgg aagctatttt cataaactct tctacatctt tcacacataa
      721 cgccacacct tttgcccaga attcttcttt cgtgatatct tccccgagat gcttcatcgc
      781 aagatcttcg actgacatga cggccgtatc gcgcaatagg gccatatatt tctgttcaaa
      841 cgattcccct tctgcaatgg ctttcgcata aatgctcagc gagaacaagt atccgaacgt
      901 atacgggaag ttatagaatg ggactcccgt aatatagaag tgcagcttcg aagcccagaa
      961 atgcggatga actgtatcaa gcgcccctgc atatgcttct ttttgcgctt cagccatcaa
     1021 ttcattcaat cttgcagctg gtacaacccc atttttcctt tcctcataaa agcgtgtttc
     1081 gaacaggaat cgtgcatgta tattcatgaa gaatgccacg ctgcgctgga ttttatcctc
     1141 cagcaaagca agtttttcct cttccgtttc tgccgctttg acagaagcgt cagcgacgat
     1201 catttccgca aatgtggatg ccgtttctgc gacgcccatt gcatattgac ggttcatata
     1261 atgcactgga cgcaatacat atgaatggaa cgcatggcca agttcatgtg ccaatgtcgc
     1321 aacgttggac atggagccgc tgtacgtcat gaaaatacgg gattgctctg aaaccggcat
     1381 gcctgtacag aagccgccag gacgtttatc agcgcggtct tccgcttcga tccagctatc
     1441 ttcaaatgct tttcttgaaa acgattccag ttccggaccg aactttccga agtgttccaa
     1501 aatgaactct gcgccttcct ggaaaggcat ttccttcgtt gatgaagtga cgggtgcatc
     1561 taaatcatac caattcatat tttcatttcc taatagttca gctttacgct tcaagtattc
     1621 tgcaaatggt gctttattgg cactgatggc cccccacatt gcatcaagcg tttcctggct
     1681 catccgattc cgcatgagag gctccttcaa aacggaatcc cagccgcgct tctcatagac
     1741 agccaagcgg aagcccgcca gatggttcaa tgcggatgca aagaaatcct ctttggattc
     1801 ccaagcctct tcaagctttt caaacgcctc tttacgcact tcgatatttg catgggaact
     1861 taagttattc gcctggccga cggaaagctt cttctcttcc ccgtcaacgt tcatttcgat
     1921 tgaaatgtcg cctacgagct ggtcgtaaag ttgtccccag gcttggtagc catcgacact
     1981 taatgcagtg atcaggcttt cttcttcctc ggataatttc aattggactt cttcgcgcca
     2041 ttcctggaga acaaaatcga attgtgacag ttgctctgtc gtaagcaatt cattccaaac
     2101 agtttcttcc gtcttggcga cgacctgttt gattttcaaa atggctgtct ggaatctggc
     2161 tcccaacgat cccacttcag cttggagaag aagcgctttt ttatctttcg tattagcagc
     2221 gatgaaacag ccgatgaccg cgcccgcttc ccttaagttt ttagcagtgt ttttgatttc
     2281 atcaataaca tgaaggattt gatccgcgtc ctccacggat gtaggcgttt gcgtggctgc
     2341 tgcaaggttt tccaatgcgc caatctgttc ttttacggcg tcaagatgtg aacggagctc
     2401 ctctgaatcg cttccgcctt taaaaaacac ttctaaatcc catacttgtg aatacgtctt
     2461 tgtcgtcgtc aatgataatc cccctaatca tttagaaaat tgtaataaaa tcatgttttt
     2521 attataccat gaatcttcgg gattcgaaat aatggaattg tgacggaaac ttcatacttt
     2581 ccttcgcaca gttccctttt cctatgtata ctgttcagag ggaggatgta ctatgtcaag
     2641 aatacttctg atgaccctgt cgctatgtac agtcatacta ttgaatatta cagcaagctt
     2701 ctactcactg aatggaaaca caacaggcga aattacagat cgtctgccac ttcttttctt
     2761 gccggcggac tatgtattga tcatctgttg gttgctctat atcatgatct ttttctggat
     2821 ttacaatttc aaaaagactt gccacaagat gagctccgaa catcgtaacc gcgtcacatt
     2881 ccttttcgta ataagttgcc ttctgcatat tgcttggatt ttcctttggc actttgaatt
     2941 gttcacgggt accgtcataa cgaaaatggc acttcttttt gcgttgctga ctttctactt
     3001 cacattcccg aagcaggata atcaaatgct tggcagagcg ccattcagtt tgctgacggg
     3061 atggatcttc atcacaacca tctcgaatgt cagttatttg cttatgatcc atggttggtc
     3121 cggattcgga ctgagcgatc cgttatggac agtcatcttt ttaacggttt caactgcgtt
     3181 cgctttacat tttatgtacc atcacaaaga ttatgtgatg aatctcgtat tcgtttgggc
     3241 attcatcggc attgctgtga ggaacgggac tgaagagctg ttcatatccg cagcggccat
     3301 ctttttatct gccgtcatcg tcacgtgcat cttcctgttt tcaggtagac gcacggacaa
     3361 taaaaataga gtaggcgcat gattttatca tatgcctact ccttttttat gctctttgaa
     3421 taagtctagg tgcggaagtg aataaaatga ctgctacgat tgc
//
LOCUS       DASULI010000142         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1816711,
            whole genome shotgun sequence.
ACCESSION   DASULI010000142 DASULI010000000
VERSION     DASULI010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816711"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFX34_03105"
     CDS             <1..199
                     /locus_tag="VFX34_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563925.1"
                     /translation="TLAFIFLVACHAVVEDTLIFVPLGIPVLPLLIIRLVTAFVLTIV
                     VAYMWNQAERKREKEVTVLDG"
     gene            192..842
                     /gene="ppaX"
                     /locus_tag="VFX34_03110"
     CDS             192..842
                     /gene="ppaX"
                     /locus_tag="VFX34_03110"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphatase PpaX"
                     /protein_id="HEX5563926.1"
                     /translation="MDKITTLLFDFDGTLLDTNELIIQTFHTVLNAHYPGRYEREDIL
                     PFLGPTLRETFESIDPEKTEQLMAEYRTWNIANHDAFAIEFDGVSETLRQLKNVGFKM
                     AIVSTKRNDMVMKGLRLMEAEDLFDTIVGLDDVEHAKPHPEPLLVAMERLNSTPEETL
                     MIGDNMHDILGGKNAGVRTAGVAWSAKGEQFIREQNPDYVLQHISDLLHITAGVEA"
     gene            839..1378
                     /locus_tag="VFX34_03115"
     CDS             839..1378
                     /locus_tag="VFX34_03115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003201873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX5563927.1"
                     /translation="MRRTERYRAEGNANSLWQIYKTVSFFKVAKNFIFIQIGRYTPFI
                     PLKNFIYRTFLGMKIGEKTSFALMVMPDTMFPERISVGNNSIIGFNTTILAHEYLIEE
                     YRIGDVVIGDRVMIGANTTILPGVEIGDGAIVSAASLVNRDIPAGAMAGGNPVRIIYT
                     AEQLEERRRNAQQLDIDKK"
     gene            1470..>2702
                     /locus_tag="VFX34_03120"
     CDS             1470..>2702
                     /locus_tag="VFX34_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5563928.1"
                     /translation="MDKKYKNIDKKVVSFIPDGEFYYQKGVKAMQRERFDDAHKYLQR
                     AVELSPNDPLILMQYGILIMEEGMFEEAYEILAAAHGLDPEEEDIIFYLAEVHAHLGL
                     LRDAKIYAEKYIALAPDGPLADDSMEIIDFAEQEEGFFNDEEMPDGEVYFLQEKARRL
                     MEEGSFEEAIEILESIIVDYPDFWAAYNNLALAYFYIGKIEPAKELLFDVLEKNKGNL
                     HALCNLAVFYYYEKDKEELDSMLELLMKIKPYQFVHRYKLGATFALVGKHKEAYGWLR
                     SLQKRGFEGDAGYYFWLAHSAYFSGNEQVAKESYERLVELDPSKAGFEPWKDVHPSSK
                     PESYEQDREFLLNKITNKYRSERLLGFYLLGKSAHKQEIVSHPTYIDLDKLSVIEQLF
                     LAHSLDYEFTTENEFDHSF"
BASE COUNT          745 a          579 c          701 g          677 t
ORIGIN      
        1 gacgctcgct ttcattttcc tcgtagcgtg ccatgccgtc gtcgaagata cactcatttt
       61 cgtacctttg ggcattccgg ttctgccgct gctcatcatc cgccttgtca ccgcgttcgt
      121 attgacgatt gtggtcgcgt acatgtggaa tcaggcagaa cggaaaagag agaaggaggt
      181 tactgtttta gatggataaa atcacaacat tgctatttga cttcgatgga acgttgctcg
      241 acacgaatga gctcatcatc caaacgttcc acacggtttt gaacgcccat tatccgggaa
      301 gatacgaaag ggaagacatc cttccatttc ttggaccgac actgagggaa acgttcgaaa
      361 gcatcgaccc ggaaaaaacg gaacaactga tggccgaata ccgtacatgg aatatagcga
      421 atcacgatgc gttcgccatc gaattcgacg gggtgagcga aacgttgagg cagttgaaaa
      481 acgtcggctt caaaatggca atcgtctcaa cgaaacgaaa tgatatggtc atgaaaggtt
      541 tacggctcat ggaggccgag gatctgttcg acacaatcgt cggcttggat gatgtggagc
      601 acgcgaagcc gcatccggaa ccgctgcttg ttgcaatgga gcgattgaat tcgactccgg
      661 aggaaacgct gatgatcggc gataacatgc acgatattct cggtgggaaa aatgccggtg
      721 ttcgcacggc cggggttgct tggtctgcta aaggcgagca attcatccgg gagcaaaacc
      781 ctgattatgt cttgcagcat atttcggatt tgctgcacat cacagcaggc gtggaagcgt
      841 gagacggacc gagcgatatc gtgccgaagg aaatgcgaac tccctttggc aaatatacaa
      901 aacggtttca ttttttaaag tggcgaagaa cttcattttc atccagatcg gacggtatac
      961 gcctttcatt ccgctgaaga atttcatcta tcggacgttt ttaggaatga agataggaga
     1021 gaagacatca ttcgccttga tggtcatgcc ggacacgatg tttcctgaac gtatttccgt
     1081 cggcaacaat tcgatcatcg gattcaatac gacgatcctc gcccatgagt atttgatcga
     1141 ggaataccgg atcggtgacg tcgtcatcgg tgaccgggtg atgatcggtg ccaatacgac
     1201 catccttccc ggcgtggaaa tcggggatgg cgccatcgta tcggcagcgt cgcttgtcaa
     1261 ccgtgatatc cccgcaggcg ctatggcagg cggcaatccg gtgcggatca tttataccgc
     1321 ggagcaattg gaggagcgtc gaaggaatgc ccaacaattg gacattgaca agaaatagga
     1381 tgacggaaat cgaagttatg atataatgaa gccagttgat tggagggacg tgtcatgcgt
     1441 tccttctaat ttttttcgga ggaatttgat tggacaaaaa gtataagaac atagataaga
     1501 aagtcgtttc ttttatcccg gacggtgaat tctactatca gaaaggcgtt aaagcaatgc
     1561 aacgcgaacg cttcgacgat gcccataaat acttgcagcg ggctgtcgaa ctgagcccga
     1621 atgatccact catcctcatg caatacggca tcctcatcat ggaggaaggc atgttcgagg
     1681 aggcctatga aattttggcg gcggcacatg gcctcgatcc ggaagaagag gacatcatct
     1741 tttacttggc ggaagtccat gcacatctgg gccttcttcg tgatgcgaag atttatgcgg
     1801 aaaagtatat cgcattggca ccggacggac cattggctga tgattcgatg gaaatcatcg
     1861 actttgctga gcaggaggaa ggctttttca atgacgagga aatgccggac ggcgaagtgt
     1921 atttccttca ggaaaaagca agaagactga tggaagaggg ctcgtttgaa gaggctatcg
     1981 aaattttgga gtcgatcatt gtcgactatc ctgatttttg ggctgcctat aataacttgg
     2041 cgctcgccta tttttacatc ggcaagatcg aacctgcgaa ggaattgctg ttcgatgtgc
     2101 ttgagaaaaa caaagggaat ctccatgcac tgtgtaatct cgccgtcttt tattattacg
     2161 agaaagataa agaggaactg gactcgatgc tcgagcttct catgaaaata aagccgtatc
     2221 aattcgtgca tcgctataaa ttaggggcta catttgcatt ggtcggcaag cacaaagaag
     2281 cgtacggctg gcttagaagc ctgcaaaaac ggggatttga aggagatgcc ggctattatt
     2341 tctggcttgc gcattcagct tatttttccg gaaatgagca agtggcgaaa gagtcctatg
     2401 aaaggctggt cgaactagat ccttccaagg ccggttttga accttggaaa gacgttcatc
     2461 catcgtccaa accggagtcc tatgaacagg acagggagtt tttattaaat aaaatcacga
     2521 ataaatatag aagtgaaaga ctgttaggat tttatttatt gggaaaatcc gcacataaac
     2581 aggagattgt ctcacatcct acgtacatcg accttgataa gctcagtgtc atcgagcagc
     2641 tgtttttggc gcatagcctc gattatgaat tcacgacgga aaatgaattt gatcattcat
     2701 tc
//
LOCUS       DASULI010000143         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1237500,
            whole genome shotgun sequence.
ACCESSION   DASULI010000143 DASULI010000000
VERSION     DASULI010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237500"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /gene="sigH"
                     /locus_tag="VFX34_03125"
     CDS             complement(<1..431)
                     /gene="sigH"
                     /locus_tag="VFX34_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX5563929.1"
                     /translation="MTDFGTLSDEEIVGILHDGNTDALDYLITKYRPFVRLKARSYFL
                     IGADREDIIQEGMIGLYKAIRDFKEDRLSSFRAFAELCITRQIITAIKTATRQKHIPL
                     NTSISLDKPVYDEESDRTLLDIISGPNMDDPADLMIHREEFS"
     gene            complement(531..1046)
                     /locus_tag="VFX34_03130"
     CDS             complement(531..1046)
                     /locus_tag="VFX34_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5563930.1"
                     /translation="MKKDILLVDGYNVIGAWKELRTLKNEKLEDARDRLLDRMAEYKA
                     NTGWRVIVVFDAHLSPGIEKLKRRHDVEVIFTRENETADERIEKLVSELSSRKVQLHV
                     ATSDLTEQWVIFAQGALRKSARELEIEMEEIDKAIAKKVQKIQEERPFSKIQLSDEVA
                     EIFEKWRRGIK"
     gene            complement(1043..1798)
                     /gene="rlmB"
                     /locus_tag="VFX34_03135"
     CDS             complement(1043..1798)
                     /gene="rlmB"
                     /locus_tag="VFX34_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5563931.1"
                     /translation="MTEKETELIGGKNPVAEALRSGRELNKIWIAEGLQKGSIGEILS
                     LAKKQGVLVQQVPKKKLDQLLDVNHQGIIASVAAYSYAEFDDLFALAESRGEDPFFLI
                     LDELEDPHNLGSILRTADATGVHGIIIPKRRAVGLTGVVAKASTGAIEHVPVARVTNL
                     SQTVEELKGRGVWIAGTDAKGSVDYRKMDATLPLAVIIGSEGKGMSKLLKEKCDFLYH
                     LPMRGHVTSLNASVAASILMYEVLRKRHPEERQ"
     gene            complement(1795..>2017)
                     /locus_tag="VFX34_03140"
     CDS             complement(1795..>2017)
                     /locus_tag="VFX34_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HEX5563932.1"
                     /translation="GFLTEEEQAIFRRGRNTKSGSVPKNTDVQTYNASSGFEAILGYL
                     HLRGDELRIQDILEESIRFIEQPKEEGTS"
BASE COUNT          468 a          548 c          406 g          595 t
ORIGIN      
        1 gagaattctt cacgatgaat catcaggtct gccggatcat ccatgttcgg ccctgatata
       61 atgtcgagga gtgtgcgatc tgattcctcg tcatacacag gcttatccag tgaaatcgaa
      121 gtattgagcg gaatgtgctt ctgacgcgtt gcagtcttga ttgctgtaat gatttgcctc
      181 gtaatgcaca gctccgcaaa tgcccgaaac gaactgagac gatcttcctt gaagtcacga
      241 atcgccttgt acaagccaat catgccttcc tggatgatat cttccctgtc cgccccaatc
      301 aaaaagtatg aacgggcctt tagccgcaca aatggacggt atttcgtaat caagtaatct
      361 agtgcgtccg tattgccgtc atgaagaatc cccactattt cctcatccga caacgtaccg
      421 aaatctgtca ttccgtcccc aatatacccc ttttccacca actgaatcac cccggtccct
      481 cataggtagt agtccaagta taccgtatgg aaaaattttg cgtcaaccgt tcattttatt
      541 cctcgacgcc atttttcaaa tatttctgcc acttcgtccg agagttggat tttagaaaag
      601 gggcgctctt cttggatttt ttgaactttt ttcgcgatgg ctttatcgat ttcttccatc
      661 tcaatttcaa gttcccgggc tgacttccgg agtgcacctt gtgcgaaaat gacccattgc
      721 tctgttaaat cggaggttgc cacatgaagt tgaacctttc tgctgctcaa ctctgaaact
      781 agcttttcaa tgcgttcatc tgccgtttca ttttcacgtg taaagatcac ctcgacatca
      841 tggcgacgtt ttaacttttc aataccgggc gataagtggg cgtcaaacac aacaatgacg
      901 cgccaccccg tgttcgcctt atattcagcc attcgatcca gcagccgatc tcgtgcatct
      961 tcgagttttt catttttcaa cgtgcgtaat tctttccatg ctccaattac attgtagcca
     1021 tcgaccagta aaatatcctt cttcattgcc tttcttctgg gtgccgttta cgcagcactt
     1081 cgtacatgag gatggaagca gcgaccgacg catttagtga cgttacgtgc cctcgcattg
     1141 gcaaatgata gaggaaatca cacttttctt tcagaagctt cgacatccct tttccttcac
     1201 tgccaatgat gaccgctaac ggcaaagtgg catccatctt tcgataatca accgatcctt
     1261 ttgcatctgt tcccgctatc catacgccgc gtccctttaa ttcttcaact gtttgggaaa
     1321 gattcgttac ccgggcgacc ggtacatgct cgattgcccc ggtagacgcc tttgctacaa
     1381 cgcctgttaa accgaccgct ctccgtttcg ggataatgat cccatgtaca cctgtcgcgt
     1441 cagctgtccg gaggatggac cctaaattat gcggatcctc aagctcgtcc agaatgagga
     1501 aaaacggatc ttctccccgc gattccgcaa gcgcaaacaa atcgtcgaac tctgcgtaac
     1561 tataggcggc gaccgatgca ataatgcctt gatggttaac gtccaatagc tggtcgagtt
     1621 ttttctttgg aacctgttgc acgagcacgc cttgcttttt ggcaagcgaa agaatttcgc
     1681 caatactccc tttttgcaat ccttcggcga tccagatttt atttaattcc ctgccagacc
     1741 gtagtgcctc agcgaccgga ttctttccgc caatcaattc agtttctttt tccgtcacga
     1801 cgtcccctcc tccttcggct gttcaatgaa ccggatggat tcttctagta tatcttggat
     1861 acgcagttca tccccgcgta aatgcaagta cccgaggatg gcctcgaagc ccgaactcgc
     1921 attgtatgtc tgcacatctg tattctttgg cacggatccg gattttgtat ttctacctct
     1981 acggaaaata gcttgttcct cttccgttaa gaaacca
//
LOCUS       DASULI010000144         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_215209, whole
            genome shotgun sequence.
ACCESSION   DASULI010000144 DASULI010000000
VERSION     DASULI010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215209"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFX34_03145"
     CDS             complement(<1..687)
                     /locus_tag="VFX34_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX5563933.1"
                     /translation="MRDQAEALRMKMLKAQGGLAKSIAIVSGKGGVGKSNFSTNFAHS
                     LRLKGKKVIVVDMDIGMGNIHILLGVAPPYSLKDYLTGQQSLENVINTDEQGLTFISG
                     GSGLDTVLDWSEEMFDRLLSAFEHLQKEYDFILFDMGAGATQRSIELIIAVDEVIVIS
                     TTEPTSITDAYSMMKFICLKDPEKKFSIVSNRVSRLDDGNDAVTRLQYAMRKFLDKET
                     SILGFLPEDAA"
     gene            complement(680..1792)
                     /gene="flhF"
                     /locus_tag="VFX34_03150"
     CDS             complement(680..1792)
                     /gene="flhF"
                     /locus_tag="VFX34_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX5563934.1"
                     /translation="MKMKKYTADTMVEAMKKVRADFGEDAVILSSNVITSKGFLGFFR
                     KKSVEVVAGFDEPLHPPSEPLYVPKQAYGFAEPKQDAEIRKEMNEMKRLLQEMKQSAT
                     FVNFPDELKPLLTFLESQELSEDLILQIGNEIFTRMKEEKRDYSNEEQKEIVKAFLTK
                     EFSELPFGGISNSKKYINVLGPTGVGKTTTIAKIAARALLEDKKKIGFITTDTYRIAA
                     IEQLRTYANLLQAPVEIAYNSKDFEEAIMKMKDLDLIFIDTAGRNYKEMKFVDDLKRL
                     IDFSLDMESFLVLSVTSKEEDMKTIINRFTSFPIEKFIFTKVDETGSIGPMFNLMKQY
                     GIGTAYYTDGQEVPEDIAEADLEKMLKLLLAGAEHA"
     gene            complement(1792..>2509)
                     /locus_tag="VFX34_03155"
     CDS             complement(1792..>2509)
                     /locus_tag="VFX34_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEX5563935.1"
                     /translation="FGLPAKWITEDVKEDAEIMGYTVVDPPSVVSTHLTEVIRANAAD
                     LIGRQETKQLVDHVRETYPILVDELTPTPLSIGEIQKVLAKLLNENVSVRNLPIIFET
                     LADYSKYTSDIDLLTEYVRQSLARQITAQYSSGVDALKVLTVSGKVEKLIADHIQQTE
                     QGNYLSIDPSHSQEILESIAKEVERVALMDQSPVILCSPAIRMYLRQITERYFPQIPV
                     LSYNELEASIEVQSVGVVDI"
BASE COUNT          631 a          617 c          506 g          755 t
ORIGIN      
        1 tgcggcgtct tccggtaaaa atcctaatat ggacgtttct ttatccagga atttccgcat
       61 tgcatattgc aatcttgtca ctgcgtcatt tccgtcatcg agtctggaca ctcggttgct
      121 gacgatactg aatttctttt cagggtcctt cagacagatg aatttcatca tggagtaggc
      181 atctgttatt gacgttggct ctgtcgtcga gatgacaatc acttcatcca cggcaatgat
      241 cagttcaatc gagcgttgcg tggcacctgc ccccatatca aagaggatga aatcatattc
      301 cttttgcaag tgttcgaaag cgctcagcaa tctatcgaac atctcctcag accaatcgag
      361 cacagtgtcc agtcccgatc cgcctgaaat gaaagtcagg ccttgctcat ccgtattaat
      421 gacattttcc aaactttgtt gaccggtcaa ataatctttc agactatatg gaggagctac
      481 accaagtaga atatggatat tgcccatccc gatatccata tcaacgacga tgactttctt
      541 ccctttcaaa cgcaaggagt gggcgaagtt cgtcgagaaa ttcgacttgc ccactccccc
      601 tttcccactt actatcgcaa tggatttggc caagccgcct tgggccttca gcattttcat
      661 acgaagtgct tccgcctgat cacgcatgtt cagcacctgc caataacaat ttgagcattt
      721 tctcaagatc cgcttcagct atatcttcag gcacttcctg accatccgtg taataggcgg
      781 taccgatgcc atattgtttc atcaagttga acataggacc gatcgatccc gtttcatcga
      841 ctttcgtaaa gatgaacttt tcaataggaa aggaagtgaa cctattgatg atcgtcttca
      901 tatcctcttc tttcgaagtg accgataaga cgaggaagga ttccatatcc aatgaaaagt
      961 cgatcaaacg ctttaaatca tccacgaatt tcatttcttt ataatttcgg cccgccgtat
     1021 ctatgaaaat caagtcaaga tctttcattt tcataatagc ttcttcaaag tccttgctgt
     1081 tatacgcaat ttcaacaggt gcctgcagca ggttcgcata tgtgcgcaat tgttcgatag
     1141 cagcaatccg atacgtgtct gtagttatga atccgatttt ctttttatct tccaaaagag
     1201 ctctagcggc aattttagcg attgttgtcg tctttccgac tccggtcgga ccgaggacat
     1261 taatatattt tttggaattc gatatccctc cgaaaggcag ttccgagaat tctttcgtta
     1321 agaaagcctt cacgatttcc ttctgctcct cgtttgaata atccctcttt tcctctttca
     1381 tccgtgtaaa gatttcattg ccgatttgaa ggatcaggtc ttcggataac tcctgacttt
     1441 ccaggaaagt gagcaacggc ttcagctcgt ccgggaaatt gacgaatgta gcggactgtt
     1501 tcatttcctg aagcagccgt ttcatttcat tcatctcttt tctgatttcc gcgtcctgct
     1561 taggctctgc aaacccataa gcctgtttag gaacgtagag cggctctgac ggaggatgta
     1621 acggctcatc gaagccggca acaacttcaa ccgatttttt acggaagaat ccgaggaacc
     1681 ctttggatgt gatgacattt gagctaagga tgacagcatc ctccccgaaa tcggctctca
     1741 ctttcttcat cgcctccacc atcgtgtcag ctgtatattt tttcattttc atcagatatc
     1801 caccaccccg acactttgga cttcaattga agcttcgagc tcattgtagg ataaaacagg
     1861 tatttgcggg aagtaacgct ccgttatttg ccttaagtac atccggatcg ccggcgagca
     1921 aagtatgaca ggtgattgat ccatcaacgc caccctttcg acttcttttg caatcgattc
     1981 taatatctct tgagaatgag atgggtcgat cgacaagtaa ttgccttgct ccgtctgctg
     2041 gatatgatcc gctatcaatt tttcgacttt gcctgaaaca gtcaacactt ttagtgcatc
     2101 gactccggat gaatattgtg ctgtgatttg tctagcaagc gactgtcgga catattcagt
     2161 tagcaggtcg atatccgatg tatacttcga ataatccgcc agtgtctcga aaatgatcgg
     2221 caaattccgt acggagacat tttcattcaa taatttagcc aacacttttt gaatctcccc
     2281 aatggaaagt ggtgtcggtg tcaattcatc caccaggatc ggatatgtct cacggacatg
     2341 atcgacaagc tgttttgttt cttggcgccc gatcagatca gctgcattcg ctcggatcac
     2401 ttctgtaaga tgcgtcgaca cgacgctagg aggatcgacg acggtgtatc ccataatttc
     2461 agcatcttct ttgacatcct ccgtaatcca cttcgcagga agaccgaat
//
LOCUS       DASULI010000145         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_39801, whole
            genome shotgun sequence.
ACCESSION   DASULI010000145 DASULI010000000
VERSION     DASULI010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39801"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFX34_03160"
     CDS             <1..184
                     /locus_tag="VFX34_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX5563936.1"
                     /translation="SVGLPVEGIALVLGVDRLLDMVRTAVNITGDAACAVVVAQTEGV
                     LGDPNDDRMPAGTKTA"
     gene            complement(290..1207)
                     /locus_tag="VFX34_03165"
     CDS             complement(290..1207)
                     /locus_tag="VFX34_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5563937.1"
                     /translation="MVRANTNNRYYIQQVSDMTGLSKQVIRKWEERYNLIRPERLPNG
                     YRIYSEKDINLLLHVKTLSEEGMPIRQAVQLAEKMRGETVPSPKEPSLRMDSLNEFVL
                     QLLEKGNRCDELELMITLQQANYEIGLAALLDQVITPFLQEVGDRWERGEWDEYQESV
                     SSLAIRDYLVQLRRNNQHHPEAPVVLAACLPYERHEIPLHIVLLQFMLKGWRTFLVGA
                     SPAPGSIEAVIQKIRPDIVILSASTSIPFEEEPDLLEKLDAFAAKQPNCQFFIGGKGA
                     VAQKAAKELYTIRLTDDFEDVWESIEIGK"
     gene            1421..2485
                     /locus_tag="VFX34_03170"
     CDS             1421..2485
                     /locus_tag="VFX34_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563938.1"
                     /translation="MFLAWNEIQKNKLRFVLITGVLMLVAYLVFFLSGLATGLKDLNR
                     ESVDKWKASSIILTEESDKSLYQSSMFIEQAEKVKAEEIAVLGQLSAIAGNGNQKTNV
                     SLFGIRPDEFIMPEVTEGKPFAGMNEVIAANSLKEEGFSIGDELHLSSSTEKLTIVGF
                     TDKAWFNAAPVLYADLDTFHKVKYGEGAEANNDRINGIVIRNGGGSVIQDDALQVIEI
                     EEFIQNLPGYTEQNLTLTFMIYFLFAISAVIVAIFLYVLTVQKISMFGVMKAQGISNY
                     YLSKSVIAQTFLLAAIGVMIGLALTLSTGAFLPAAVPVSFDFPTMLIYGAVLVVVAIA
                     GAVFSVWTIVRVDPLKAIGG"
     gene            2489..>3023
                     /locus_tag="VFX34_03175"
     CDS             2489..>3023
                     /locus_tag="VFX34_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563939.1"
                     /translation="MRVLELKHVTKTFGSGHKKVDALKKTEFQASRGELIAIIGPSGS
                     GKSTFLSIVGGLLTPTSGDVILNGQNVTTMTEKERAQIRLNEIGFILQASNLIPFLTV
                     AKQMKLLNKVKKGNMNVDEMERLYKDLGIGDLRGKYPSDLSGGERQRVAIAKALYSNP
                     SIILADEPTASLDSDRAY"
BASE COUNT          801 a          654 c          763 g          805 t
ORIGIN      
        1 atctgtcggc ctgcctgtcg aagggattgc actcgtctta ggagtcgacc ggctgctcga
       61 tatggtgcgg acggctgtca acatcacggg agatgcggct tgcgcagttg tcgttgcgca
      121 gacggaggga gtcctcggtg atccgaatga tgaccggatg ccggctggta cgaagaccgc
      181 ctgattcctc ggcaaaataa tcaaaagcat aaataagagc tgtccagaaa gtcagttaaa
      241 actgatttac tgtgacagct ttttacttgc taacaaaaaa gcagtagaat tactttccaa
      301 tttcaatact ctcccaaaca tcttcgaaat catccgtcag gcggattgta taaagctctt
      361 ttgcagcttt ttgtgcgacc gctcctttgc cgccgatgaa aaactggcaa ttgggctgct
      421 ttgccgcgaa tgcatcaagc ttctccaaca ggtcaggctc ctcttcaaaa gggatggaag
      481 tggacgccga gaggatgacg atgtcaggcc ggatcttttg gatcactgcc tcgatggaac
      541 cgggggcggg ggaagcgccg actagaaagg tgcgccatcc cttcagcatg aattgtaaca
      601 ggacgatatg aagcgggatt tcatgccgtt cataagggag gcacgcggcg agcaccaccg
      661 gggcttccgg atgatgctga ttgtttctgc ggagctgtac gagataatca cggatggcga
      721 ggcttgagac cgattcctgg tattcatccc attctcctct ttcccatcga tcaccgactt
      781 cttgtaaaaa aggtgttatt acttgatcca acaaggcggc tagcccgatt tcataattgg
      841 cctgttgaag ggtgatcatc aattcgagtt catcgcatct attccctttt tccaataatt
      901 ggaggacgaa ctcattcaaa ctgtccatcc ggagcgatgg ttccttgggg gagggaactg
      961 tctctcctct cattttctca gccagttgaa ccgcttgccg gatcggcatg ccttcttcag
     1021 aaagtgtttt gacgtgcaag aggagattga tgtctttctc gctatagata cggtagccgt
     1081 ttggaagccg ttcggggcgg ataagattgt accgctcttc ccatttgcgg atgacctgct
     1141 tggaaagccc cgtcatgtct gaaacctgct ggatataata acgattattt gtattggctc
     1201 ttaccaaacc tgcgcctcct aacgaatgtt cattgcgtaa agtatagcgg aaattacggg
     1261 tttgtacaat gttggatggt taaccttata catgactgtt aatgtggtgt acactatttt
     1321 tgttttaacg ctatacaaac ctttgacaac cggttctcat ctcttcataa tgaacataca
     1381 atcattgaat aaataataga agcggcaagg agtgtgtaaa atgtttctcg cgtggaatga
     1441 aatccaaaag aataagctaa ggtttgtact gatcacgggt gtcctcatgc tagttgctta
     1501 tctcgtcttc ttcctgtcag gcctagcgac aggattgaag gatctgaacc gggaatcggt
     1561 tgataaatgg aaagcgtctt caattatctt aacggaggaa tcggataaga gtctctatca
     1621 atcctctatg ttcatcgagc aagctgaaaa ggtgaaggct gaagaaatcg cggtcttagg
     1681 ccaactgagt gcgattgccg gaaatggtaa tcaaaagaca aatgtatcac tctttggaat
     1741 tcgaccagac gaattcatca tgccggaagt gacggaaggc aagcctttcg cgggaatgaa
     1801 cgaagtgatc gcggccaatt cattaaaaga agaagggttc agcattgggg atgaacttca
     1861 tttatcatcc tctacagaaa aattaacgat tgtcggcttt accgataaag cctggttcaa
     1921 tgcggcgccg gttctttacg ccgaccttga cactttccac aaagttaagt acggagaagg
     1981 tgcggaagca aataatgatc gcatcaatgg aattgtcatc cggaatggag ggggtagtgt
     2041 cattcaagac gatgctttgc aagtcattga aatagaagaa ttcattcaaa acttgcctgg
     2101 ctacaccgaa cagaatttga cgttgacgtt catgatctat ttcctatttg ccatatcagc
     2161 agtcattgta gccattttcc tttacgtgtt aactgtccag aagatcagta tgttcggggt
     2221 gatgaaagcg caagggatct ccaattatta tttatcgaaa tcggtcattg cgcagacctt
     2281 tctattagcg gcaatcggcg tcatgatcgg attggcgcta acattgtcaa cgggggcatt
     2341 cctaccagcc gctgttccag tctcctttga ctttccgacg atgctcattt acggggcagt
     2401 tctcgtcgtc gtagctattg cgggagccgt tttttcagta tggacgatcg tccgggtaga
     2461 tccattgaaa gcgattgggg ggtgaatgat gagagtactg gagctaaaac atgtaacgaa
     2521 aacttttggc agcggccata aaaaggtgga tgcgctgaag aaaacggaat tccaggcaag
     2581 ccgaggggaa ttaattgcaa ttatcggacc ttcaggttcc gggaaaagta cgtttctaag
     2641 cattgtcggg ggattgctta ctccgacttc cggggatgtc atcttgaatg ggcagaatgt
     2701 cacgacgatg actgaaaaag agcgtgcgca aattcggttg aatgaaatcg ggttcatcct
     2761 tcaagcttca aaccttatcc cttttttgac agtggcaaaa caaatgaagc tattaaacaa
     2821 agtgaaaaag ggcaatatga atgtagacga gatggaacgg ctttataaag acttaggcat
     2881 tggcgacttg cgaggcaagt acccatccga cttgtccggt ggtgagcggc aacgtgtcgc
     2941 cattgcaaag gccttatata gcaacccgtc tattatcctg gctgatgaac cgacagcatc
     3001 tttagactct gaccgggcgt acg
//
LOCUS       DASULI010000146         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_459827, whole
            genome shotgun sequence.
ACCESSION   DASULI010000146 DASULI010000000
VERSION     DASULI010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459827"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFX34_03180"
     CDS             <1..217
                     /locus_tag="VFX34_03180"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5563940.1"
                     /translation="PLLREFDHIRTCCRELGAYGMTVSGAGPSIFVAVKEGTQEEIAG
                     RLEKEFPYYRAIAVTPAPIGSTIQKDA"
     gene            complement(310..402)
                     /locus_tag="VFX34_03185"
     tRNA            complement(310..402)
                     /locus_tag="VFX34_03185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Ser,seq:tga)
     gene            655..923
                     /gene="ffs"
                     /locus_tag="VFX34_03190"
     ncRNA           655..923
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFX34_03190"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1211..2977
                     /gene="dnaX"
                     /locus_tag="VFX34_03195"
     CDS             1211..2977
                     /gene="dnaX"
                     /locus_tag="VFX34_03195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5563941.1"
                     /translation="MVYQAFYRVYRPQSFSEMSGQRHVKQTLQNALQHNKTSHAYLFS
                     GPRGTGKTSAAKVFAKALNCENGPASEPCNECPTCISVTEGSNTDVIEFDAASNSRVE
                     EIRDIIEKVRFAPSNARFKVYIIDEVHMLSNSAFNALLKTLEEPPPHAVFILATTEPH
                     KLPLTIISRCQRFDFKPITSAEIVDRMKQVLDDAGIGADEGALKVIAQAASGGMRDAL
                     SMLDQVVSFSGDRLTAESALLVTGSIGEDIFYQLAEALLSKDAAAALTLLDQLIAEGK
                     DVSRLAEDLITFFRDLLLLRTAPDLIELLELISGDERFVSMSNAFEPETLYSFIDILS
                     KTQQEMRFSNHAKVYVESALLKMIHLEGPAVQAGGNQAIDPHLAQKVESLERVIADLQ
                     QQLANGVRPAEGAPAAAQPKRNASKTSQSFRIPTGKINEVLKGATRQDIQVIREEWAT
                     MMQTLQRSHSALLEETEPVAASDNAFVLKFKYEIHCLMASENSSLRSGLSEALRSRTG
                     KAYDVVYVPEEGWLKVREEFIRNNGLDKQQDGTDEATGEGNEDAGEVPTFVQEAEQLN
                     DDPIVAEAERMFGKEFIQVHDD"
     gene            3002..3322
                     /locus_tag="VFX34_03200"
     CDS             3002..3322
                     /locus_tag="VFX34_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5563942.1"
                     /translation="MRGMGNMQGMMKQMQKMQKKMAEAQEELAEKRLEGTAGGGMVKV
                     IVSGQKEVLEVVIDPSVVDPEDVEMLQDLVVIATNEAMKQADELTNSTMGQFTKGLNL
                     PGMF"
     gene            3334..3930
                     /gene="recR"
                     /locus_tag="VFX34_03205"
     CDS             3334..3930
                     /gene="recR"
                     /locus_tag="VFX34_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5563943.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAGRLAFFVLSMKEDTVLEFA
                     KALVDAKRNLRFCSVCGHITDIDPCQICQDQSRDRSMICVVQDPKDVIAMEKMRDYNG
                     MYHVLHGAISPMDGIGPEDINVPSLLTRLQDEEVEELILATNPTIEGEATAMYISRLV
                     KPSGITTTRIAHGLPVGGDLEYADEVTLSKALEGRREL"
     gene            3944..4162
                     /locus_tag="VFX34_03210"
     CDS             3944..4162
                     /locus_tag="VFX34_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019712989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HEX5563944.1"
                     /translation="MFGRKRKLKREFDAQLYNLLLDTKEEWERAREIEKYLNDYDQEV
                     MVRKKIAESKYFHLFKEAKERQLRIDRG"
     gene            4251..4514
                     /locus_tag="VFX34_03215"
     CDS             4251..4514
                     /locus_tag="VFX34_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HEX5563945.1"
                     /translation="MKIIGIAIIGVSLLFLLMMDKKQIKKLSERLSVFWFRLAFAFLA
                     LFMLNIVGGFIGIYFPVNIASGLLLAILGIPGLAALFAFAVFL"
BASE COUNT         1414 a         1056 c         1304 g         1144 t
ORIGIN      
        1 accattactc cgagagtttg atcatatacg cacttgctgc cgtgaattgg gtgcgtacgg
       61 gatgacagtc agcggtgctg gcccttctat tttcgttgca gtaaaggaag ggacacagga
      121 agagattgcg ggacggctcg aaaaggaatt tccatattac agagcgattg cagttactcc
      181 tgctcctatc ggttccacaa tacaaaaaga tgcctagcca atggatggcg gggcatcttt
      241 tttcataaaa aaaatccatt acatttaatg taacagactt ctccaactta tgcgcttttt
      301 taaaatgaat ggcggaggaa gagggattcg aacccccgcg ggctttaaca cccctgtcgg
      361 ttttcaagac cgatcccttc agccaggctt gggtattcct ccgtatcaac agaacataat
      421 ttatcatgat tcgatggaga gtgtcaacta catatatgca ctcatcctaa gtaagtttat
      481 tcatagaagt ctgtcttttg caagggaaac gagaactatt tgggcgtaaa cgtgaactct
      541 ttaacggaga acgagaaata agtcgtcata aacgagaaat cttctcctag agaagagaga
      601 agtttcagag gggacaggtt gattttatcc ataaaaaaat gtatactaag aaagccgtgc
      661 taggtgggga ggtagcggtg ccctgtaact tgcaagccgc tctagcaaga ctgaatccct
      721 tctctgaggc tgtttccgca tgtagggtct gcctcttgca cgtagtgttg acgtctgggt
      781 cctgcgcaat gggaacccat gaaccatgtc aggtccggaa ggaagcagca ttaagtggta
      841 tttctcatgt gccgcggggc agcctaggct gagctgtcga caggagtaac gcttatgtgc
      901 ggcagtcgaa ggaaggtgca cggctttact tatacaaatt caactcgtcc gattatcggg
      961 cgggtttttt tatttctaaa gcgaaagccg tcacgaagaa cggcttttgc gagtaaaagc
     1021 gcagcgttac gagcacattg cggcctgcct agatgcgaca ggcataaggc ggacatgcga
     1081 accggcgctt tttgccgtgc agcagggctg acttatgacc cccgagcatc tggtcgacga
     1141 agctggatgt tacgttcatc tatgctataa taaaaacact attgcatcga aaaggaaagg
     1201 agggggaatg gtggtttatc aggcttttta ccgtgtctac cgcccgcagt cgttcagcga
     1261 aatgtccggc cagcgacacg tcaagcaaac attgcaaaac gccttgcagc acaataagac
     1321 gtcccatgcc tatctgtttt cagggcctag ggggacgggg aagacgagtg ccgcaaaggt
     1381 ttttgcaaag gcattgaatt gtgagaacgg tccagccagc gagccgtgca atgaatgccc
     1441 gacttgcatt agcgtgacgg aagggtcgaa tacggatgtc atcgaattcg atgccgcctc
     1501 gaactcgcgt gtggaagaaa tacgggacat tatcgagaaa gtccgattcg ccccatcgaa
     1561 tgcccgattc aaagtgtata ttatcgatga agtgcatatg ttatccaatt cggcattcaa
     1621 tgcactatta aagacattgg aagaaccgcc gcctcacgcg gtgttcatat tggcaacgac
     1681 agagccccat aagcttccgt tgaccatcat ttcccgctgc caacgtttcg atttcaagcc
     1741 gatcacatcg gcggaaatcg tcgaccggat gaaacaggtg ttggacgacg ccggaatcgg
     1801 ggcggatgaa ggggcattga aggttatcgc acaggctgcg tcaggcggaa tgcgcgatgc
     1861 actcagcatg cttgaccaag tcgtgtcctt cagcggcgat cgcctgacag cggagagcgc
     1921 attgcttgtc acaggatcga tcggggaaga tatattctac caactggcag aagcgcttct
     1981 ttcaaaagac gccgcagctg ccctgacgct gctcgatcag ctcatcgccg aggggaaaga
     2041 tgtatcgcga ttagcagagg atctgatcac cttcttcagg gatctccttc tattaagaac
     2101 ggctccggat ttgatcgaac tgctggaatt gatttcaggg gatgaacgat tcgtatccat
     2161 gtcaaatgca ttcgaaccgg aaacccttta ttcattcata gacatccttt cgaagacaca
     2221 gcaggaaatg cgcttttcaa atcacgcaaa agtgtatgtg gagtcggcat tgctgaagat
     2281 gatccatctg gagggaccgg ctgtacaagc cgggggcaat caagcgattg atccgcatct
     2341 tgcccaaaag gtggagagcc tcgaacgtgt catagccgac ctgcaacagc aactcgcaaa
     2401 cggggtgcgc cctgcggaag gtgctccggc tgccgcacag ccgaagagaa atgcttcaaa
     2461 aacatcccag tcgttcagaa taccgacagg gaaaataaat gaagtgctaa aaggggcaac
     2521 ccgtcaagat atccaagtga tccgggaaga gtgggcgacg atgatgcaga cattgcaaag
     2581 atcccattcg gcgctcctcg aagaaactga accagtagcg gcatccgaca atgcttttgt
     2641 gttaaaattc aagtatgaaa tccattgcct gatggcttcg gaaaactcct cgctcaggtc
     2701 aggcctatcg gaagccctac gctcccggac cggcaaagca tatgatgtcg tgtatgttcc
     2761 ggaggaaggc tggctgaagg tcagggagga gtttatccgc aacaatggtt tggataaaca
     2821 gcaggacggg acggacgagg caactggcga ggggaatgaa gatgcgggag aagtaccgac
     2881 tttcgttcag gaagccgaac agttgaatga cgatccgatc gttgcagaag cggaaagaat
     2941 gtttggcaag gaattcatac aagtacatga tgattaaaca attaatgagg aggaaatcac
     3001 aatgcgtggt atgggaaata tgcaaggtat gatgaagcaa atgcagaaga tgcaaaagaa
     3061 aatggcggaa gctcaagagg aactggctga aaagcgccta gaaggaacag cgggaggcgg
     3121 catggtgaaa gtgatcgttt caggccagaa agaagtgctt gaagtcgtca tcgatccatc
     3181 cgtcgtcgat cctgaagacg tggaaatgct gcaggacctt gtggtgatcg ctacaaatga
     3241 agcgatgaag caagctgatg aactaacgaa ctccacaatg gggcaattca cgaagggatt
     3301 gaacctgcct ggaatgttct aggaggaata actatgcatt atcctgaacc gatatcgaaa
     3361 ctgatcgata gtttcatgaa actgccaggc atcggcccga aaacagcggg ccggctggcg
     3421 ttttttgtat taagtatgaa agaggataca gtactcgaat ttgccaaggc gctagttgac
     3481 gcaaagcgga atctgcgttt ctgctcggta tgcgggcata ttaccgatat agatccatgc
     3541 cagatatgcc aagaccaatc gcgtgaccgg tcgatgatct gcgtcgtgca ggatccgaaa
     3601 gatgtcatcg cgatggagaa gatgcgggac tacaacggaa tgtaccatgt cctgcatggt
     3661 gcaatctccc cgatggacgg catcggacct gaagatatca atgtcccttc attattgacc
     3721 cgacttcaag atgaagaagt ggaggaattg attttggcga cgaacccgac aattgaggga
     3781 gaagcgacgg cgatgtacat ttcgagacta gtaaaaccgt cgggtattac aacgacaaga
     3841 attgcacacg gccttcctgt cggaggagat cttgaatatg cggatgaagt gactttatcg
     3901 aaagcacttg aaggacgcag ggaattatag gaggtcaccg tcgatgtttg gccgaaagag
     3961 aaaattaaaa agggaattcg atgctcagct ttacaaccta ttgctagaca caaaggaaga
     4021 gtgggagcgg gcaagggaaa ttgagaagta cttgaatgat tatgatcaag aagtgatggt
     4081 acgaaagaag atagcggaaa gtaaatattt tcatttattc aaagaagcga aagaacggca
     4141 gctccgtata gatcgcggat agaccgcggc gaaacccggg ctgacatgga gcgtgataat
     4201 ttttcagggg agcgcataga gtggactatt agtcgaaagg ggcgctttcc atgaaaatta
     4261 tcggcatcgc catcatcggt gtcagtcttc tcttcctatt gatgatggat aaaaaacaaa
     4321 tcaagaaact gtccgagcgt ctgtccgtat tttggttccg cttggcattt gcgtttctcg
     4381 cactgttcat gttgaacatt gtcggcggat tcataggaat ctattttccg gtgaacatcg
     4441 catccgggct ccttttagcc atactcggaa ttccgggctt agccgcgtta ttcgcgtttg
     4501 cggtttttct atgatggatg tctcttcttt atagaagggg cattttttta tgggggaatg
     4561 aaatgcgtaa tgaagcaaca atgaagcgaa gatgtcgaaa gaaactatct attgaatagg
     4621 gtattgcatt caaaaagaga tgctgttata gtaataaacg ttgcacttcc gctaagtaaa
     4681 taaccacttc aaaacaacct gaaacttttt gaaaaaagtt gttgacatcg gaattgcaaa
     4741 catggtatta tataaaagtt gcttctgacg gagcaacgca caatgaacct tgaaaactga
     4801 acagcaaaac gtcaacaaat aaagttctgg agccgacccc gtcggcgaaa ggaacaaacg
     4861 aatcttcgga ttcgaaattg acatcttaaa tgatgccagc aagaaactcg agctattc
//
LOCUS       DASULI010000147         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1129642,
            whole genome shotgun sequence.
ACCESSION   DASULI010000147 DASULI010000000
VERSION     DASULI010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129642"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFX34_03220"
     CDS             <1..465
                     /locus_tag="VFX34_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX5563946.1"
                     /translation="LMTNYDIGISIQGVKLQDVELPNADVRAAFTAVTDARETKSTKI
                     NEAEKYRNQRESEAIGERDAIKSRALGQKTARIEQARGDVALFNDLHAEYTKNKAITR
                     QRLIIETLEQVLPHAKIYIMNDNGETLKYLPLQQLESQTPPPAEQKTEGGGN"
     gene            516..1466
                     /locus_tag="VFX34_03225"
     CDS             516..1466
                     /locus_tag="VFX34_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX5563947.1"
                     /translation="MQRTQKKKPSGAEKVVTPKKPFNTRKFVKTAITLTVIFAIAVIL
                     LANVFIVKEGEYRVVRQFGEITRIIEEPGLNMKIPFIQSVTSLPKNQMNYNVLEAEIS
                     TKDKKRIIIDNYAVWKITDARKMISNARNIINAEARMEEFIYSVIRNEMGKLDYVDVV
                     NEENSKRGNLNDLVTQKVNEFLDEGNFGIEVIDVRMKRIDLPEENEHSIYTRMISERE
                     STAQRYLSEGDAEKKIIMAETDRGVQEMLATAKKDAAIIHAEGEAEAAKIYNNAFSKD
                     PEFYNLYRTLQSYSRTIGEDTVIIMPADSPYAKILTGYLE"
     gene            1554..>2847
                     /locus_tag="VFX34_03230"
     CDS             1554..>2847
                     /locus_tag="VFX34_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5563948.1"
                     /translation="MTKKKIVLLDGNSLAYRAFFALPLLTNDTGIHTNAVYGFTMMLQ
                     NILAEEKPTHMLVAWDAGKTTFRHSTFGEYKGGRQKTPPELSEQFPYLRKLLDAFQIT
                     QYELDQYEADDIIGTLSKSGDDEGVEVVIISGDKDLTQLASDHTTVCITRKGITDLEK
                     YTPEHVMEKYGITPPQIIDMKGLMGDASDNIPGVPGVGEKTALKLLKEYGTVEKVYES
                     LDSVSGKKLKENLKENEEQAYMSKKLATIEVDAPITISIDDLAYPGPDEDNVKTIYEE
                     LKFKTLLDKMQPQAESAPVAEIDVQIITDSESIELSDEMAIHVEMMDENYLSTEVTGI
                     SIADGEQTIFVPIGVLQQSDLLKGWLRDEGKRKFATDSKAAYASLLRYGVEVNGFEFD
                     LLLATYISNPSTSYTDVASVAKEFNYSDVQPDEAVYGKG"
BASE COUNT          954 a          552 c          681 g          660 t
ORIGIN      
        1 cttatgacga attatgacat tggcatttcg attcaaggtg tcaaattgca ggatgttgag
       61 ctaccgaatg cggacgtccg agccgcattc actgcagtta cagatgcccg tgaaactaaa
      121 agcaccaaaa ttaatgaagc cgaaaaatac aggaatcagc gggaaagtga agcaatcggg
      181 gaacgggatg caattaagtc ccgtgcgctc ggacaaaaga ctgcacgtat tgagcaagca
      241 cgtggagatg tcgctctatt caacgatctg cacgcagaat acactaaaaa caaggcgatc
      301 acacgtcagc gtctgatcat tgaaacgtta gaacaagtat tacctcatgc gaaaatctat
      361 attatgaatg ataatggcga aacgttgaaa tacttgccgt tgcagcaatt ggaaagccaa
      421 acaccaccac cggctgaaca gaaaacggaa gggggcggga actgatgtcg aacgataaca
      481 atccattcaa aagtattgaa gatagattca aagaaatgca acgtacacaa aagaaaaagc
      541 cgtctggagc ggaaaaggtt gtcactccaa aaaagccgtt taacacaaga aagttcgtaa
      601 agacagcaat tactttgacg gttatctttg caattgcggt cattctactc gcgaatgtat
      661 tcattgtaaa agaaggagaa tatcgggtag tacgccaatt cggtgaaatt acgagaatta
      721 ttgaggaacc aggattgaac atgaaaatac cattcattca atcagtgaca agcttgccta
      781 aaaatcaaat gaattataat gttttagaag cagaaatcag cacaaaagat aaaaagcgga
      841 ttatcattga taattatgct gtatggaaaa ttacggatgc caggaagatg atttccaatg
      901 cgcgaaatat catcaatgcg gaagcacgca tggaggaatt catctattcc gtcattcgaa
      961 acgagatggg taagttggat tacgtagatg tcgtcaatga agagaattca aaacgaggca
     1021 acttaaatga ccttgtcact cagaaagtga acgagtttct tgatgaaggt aattttggca
     1081 ttgaagtgat tgacgtccgg atgaaaagaa ttgatcttcc agaggaaaac gaacattcca
     1141 tttacacacg gatgatttcc gaacgtgaat cgactgctca aagatatctc tcagagggag
     1201 atgcggaaaa gaagataatt atggccgaaa cggatcgggg agtacaagag atgcttgcga
     1261 cagcgaagaa agacgcagcc atcatccatg ctgaagggga agcggaagct gctaaaatct
     1321 ataataacgc attctcgaaa gatcctgagt tctacaatct ataccgaacg ctgcagtcct
     1381 attcgcgaac gatcggcgag gatacggtga tcatcatgcc agccgattcc ccatacgcga
     1441 aaatactgac aggatacttg gaataataga tgacgtcatt ttcatttact cttgcccacg
     1501 tggtaagata aatggagatg acgttttttg aacgaacaag ggagtggagt catgtgacaa
     1561 aaaagaaaat agtgctatta gacggaaaca gcttggcgta tcgagcattt ttcgcattgc
     1621 ctttattgac caacgataca ggcatccata caaatgctgt gtacggcttc acgatgatgc
     1681 tgcaaaacat attggccgaa gaaaagccga cgcatatgct cgtcgcctgg gatgccggga
     1741 agacgacatt caggcattca acattcggcg aatataaagg cggaaggcaa aagacgccgc
     1801 ctgagctatc ggaacaattc ccatacttgc ggaaattgct ggatgccttt caaatcacgc
     1861 aatacgaact cgatcagtat gaagcggatg atatcatcgg tacattaagc aaatcgggtg
     1921 acgatgaagg cgtggaagtt gtcatcattt caggtgataa agacttgacg cagcttgcaa
     1981 gcgaccatac gacggtttgc attacgcgaa aagggattac agaccttgaa aagtacacac
     2041 ctgaacatgt catggagaaa tacggcatca cgccaccgca aatcattgat atgaaaggtc
     2101 tcatgggaga tgcttccgac aatatccctg gcgtaccggg tgtaggtgaa aagactgccc
     2161 ttaaattgct gaaggaatat ggaactgttg aaaaggtcta tgaatcattg gattccgtat
     2221 cagggaagaa attgaaggaa aacttgaaag aaaatgaaga gcaggcatat atgagcaaaa
     2281 aactagcgac aatcgaagtg gatgccccga ttaccatttc gatagatgac ttggcttatc
     2341 cgggaccgga tgaagataac gttaaaacga tttatgaaga actgaagttc aagacattgc
     2401 ttgataaaat gcaaccgcaa gctgagagtg cacctgtagc ggaaatcgat gtacagatca
     2461 ttacggattc cgaatccatt gaactgtcgg atgagatggc gatccatgtg gaaatgatgg
     2521 atgaaaatta tttatctacc gaagttacag gcatcagcat cgcggatggc gagcaaacaa
     2581 ttttcgttcc gatcggggtc ttgcaacaat ctgacctttt aaaaggatgg cttcgcgatg
     2641 aagggaaaag gaaatttgca acagattcaa aagcggctta tgcgtctttg ttgcgctatg
     2701 gcgttgaagt gaatggcttc gaattcgatc tattactggc cacctatatt tcaaaccctt
     2761 caacttccta cacagacgtc gcttccgtcg caaaggaatt caattattct gatgtgcagc
     2821 ccgacgaagc ggtgtatgga aaaggcg
//
LOCUS       DASULI010000148         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1636844,
            whole genome shotgun sequence.
ACCESSION   DASULI010000148 DASULI010000000
VERSION     DASULI010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636844"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VFX34_03235"
     CDS             <1..1072
                     /locus_tag="VFX34_03235"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEX5563949.1"
                     /translation="PGVDIVVGTQDRTKLLGLIDEFKVERQPINAVRNIMKNRVYEEL
                     DVPAFTDRTRASLKIQEGCNNFCTFCIIPWARGLMRSRDPEEVIRQAQQLVDAGYLEI
                     VLTGIHTGGYGEDLKDYNLARLLRDLETQVKGLKRLRISSIEASQLTDEVIEVLREST
                     KIVRHLHIPIQSGSDTVLKRMRRKYTMEFFAERLHRLREALPHLAITSDVIVGFPGET
                     EEEFMDTYNFIRDHRFSELHVFPYSKRTGTPAARMEDQIDEDVKNERVHRLIELNDQL
                     AKQYAAEFEDEVLEVIPEEKYKLDPESGLYEGYTDNYLKIVFPADESMVGKIVKVKIT
                     KAGYPYNEGQFVRVVESEEALA"
     gene            1183..1854
                     /gene="deoC"
                     /locus_tag="VFX34_03240"
     CDS             1183..1854
                     /gene="deoC"
                     /locus_tag="VFX34_03240"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX5563950.1"
                     /translation="MNQQYAAYIDHTLLKADATKEEITQLCEEAKTYSFASVCVNPTW
                     VKTAAELLNGSSSKVCTVIGFPLGASTSAVKAFETKDAIENGAGEIDMVINIGALRSG
                     DDELVKNDIAAVVDSAKGKAIVKVIIETSLLTDQEKRKACELSRAAGADFVKTSTGFS
                     TGGATVEDVKLMRGVVGPEMGVKASGGVRSFEDMEKMIEAGATRIGASSGVQIMQGLQ
                     GDSAY"
     gene            2003..2176
                     /gene="rpsU"
                     /locus_tag="VFX34_03245"
     CDS             2003..2176
                     /gene="rpsU"
                     /locus_tag="VFX34_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5563951.1"
                     /translation="MTKTVVRKNESLEDALRRFKRTVSKSGTIQEVRKREFYEKPSVR
                     RKKKSEAARKRKF"
BASE COUNT          715 a          452 c          613 g          568 t
ORIGIN      
        1 tcctggagtc gacattgtcg tcggtacaca ggaccgtacg aaattgctcg gactgattga
       61 tgaattcaaa gtggaacgcc aaccgatcaa tgccgtccgc aacattatga agaatcgggt
      121 atacgaagag cttgatgtgc ctgcgttcac ggatcgcaca cgggcttcat tgaaaattca
      181 ggaaggctgc aataatttct gcacgttctg tatcatccca tgggcccgcg gtctaatgcg
      241 ctccagggat cctgaagaag tcatccggca agcccagcaa ttggtcgatg caggttatct
      301 tgaaatcgtc ctaacgggaa tccacaccgg cggatatggc gaggacttga aagattataa
      361 cctagcccga ctactgcgag accttgaaac gcaggtaaaa ggtttgaaaa ggctccggat
      421 cagttccatt gaagcaagcc aattgactga cgaagtcatt gaagtgttgc gtgagtcaac
      481 gaagattgtc agacatttgc atattccgat ccaatccgga tcagacacgg tgctgaaacg
      541 gatgagaagg aagtatacga tggaattttt cgctgaacgg ctgcatcgtc tgcgtgaagc
      601 attgccgcat cttgccatca catccgatgt cattgtcggt ttcccgggtg agacggaaga
      661 ggaattcatg gacacctata atttcatcag ggatcatcgt ttttccgaat tgcatgtctt
      721 cccttattca aaacgtacgg gaacaccagc agccaggatg gaagatcaga ttgatgaaga
      781 tgtgaaaaat gaaagggtcc accgtctgat tgagctgaat gatcaattgg cgaagcaata
      841 tgccgcagag tttgaagacg aagtgcttga agtcattccg gaagagaaat ataagcttga
      901 tccggaaagc ggtttgtatg aaggatatac ggacaactac ttgaagatcg ttttcccggc
      961 tgacgagtcg atggtcggca aaattgtcaa agtgaaaatt acgaaggcgg gttatccgta
     1021 taatgaaggg caattcgtcc gtgttgtaga atcggaagag gccctagcgt aagaaaatca
     1081 agaatacagt tcatccgttg ctgggtggac tgttttttgg tgcgaaggaa atcattattt
     1141 gctatgatga aagaggtacg tacaactgga ggaggaatta gtttgaatca acaatatgca
     1201 gcatacatcg accatacatt actgaaagcg gatgcgacaa aagaggagat cacccaactt
     1261 tgtgaagagg ctaaaaccta ttcttttgct tcggtatgcg tcaatccgac atgggttaag
     1321 acagcggcgg agcttttgaa cggatcatca tcaaaagttt gcaccgtgat cggtttccct
     1381 ttaggcgctt ctacaagcgc agtcaaagcg tttgaaacga aggacgcaat tgaaaacggt
     1441 gcaggtgaaa tcgacatggt catcaatatc ggagcgctgc ggagcggcga cgatgaacta
     1501 gtcaaaaatg acattgcggc agttgtcgat tcggcaaaag gcaaagcgat cgtaaaagtg
     1561 atcattgaaa cttcattgct tactgaccag gagaaaagga aagcatgtga attgtcgcgt
     1621 gcagcaggcg ccgattttgt caagacatca acaggattct caactggcgg tgcaactgtg
     1681 gaagatgtga agctgatgcg tggagtcgtc ggtccggaaa tgggtgtcaa agcctccgga
     1741 ggagtacgca gcttcgagga tatggaaaaa atgatcgagg caggcgcgac aaggatcggc
     1801 gcaagttcag gcgtccagat catgcaaggg ctgcagggcg attctgcata ttgatccacc
     1861 agccgtggag aaaagaaatt cctatttcac aaagatattt tgaaagtttt ttattgacca
     1921 ctcgcgtata gtacgatata attttttagt acaaggagta acaccttgtt tcgaagtgtg
     1981 ctcggaggga gggacaagag atatgacgaa aactgtcgtt cgcaaaaatg aatcgcttga
     2041 agatgctctt cgccgcttca aacgtactgt atcaaaaagt ggtacaatac aagaggtaag
     2101 aaagcgtgag ttctacgaga aaccaagtgt tagacgtaaa aagaagtctg aggctgctcg
     2161 taagcgtaaa ttctaatatc tgtccatatt aactaacaaa aataagcatg ttatgaatga
     2221 aaccgggaga tccttttgga ttttccggtt tttgtacggt ttcgaatgaa actttttaac
     2281 aaggcaatac gtaaagaagg tataatggat ataaagaaag aggtgaatga acgaatgcgt
     2341 aaaatgat
//
LOCUS       DASULI010000149         5745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_429015, whole
            genome shotgun sequence.
ACCESSION   DASULI010000149 DASULI010000000
VERSION     DASULI010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429015"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /gene="argS"
                     /locus_tag="VFX34_03250"
     CDS             complement(<1..580)
                     /gene="argS"
                     /locus_tag="VFX34_03250"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5563952.1"
                     /translation="MKKDILHALQSVSPVQLEEHMLERPANSKLGDFALPCFPFAKIL
                     RKSPVAIAEEIAGSLSHPSIRKAEAVNGYVNIFLNRTAIAADLLQNIVDKSATYGSSN
                     EGNGGVVTIDLSSPNIAKPFSMGHLRSTVIGNSIALLLEKNGYQPVKINYIGDWGTQF
                     GKLLTAYRKWGNEEDVRQEPIKTLLKLYIRFHE"
     misc_binding    complement(628..843)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            942..2090
                     /locus_tag="VFX34_03255"
     CDS             942..2090
                     /locus_tag="VFX34_03255"
                     /inference="COORDINATES: protein motif:HMM:NF022953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEX5563953.1"
                     /translation="MKNILSTLFVAALLLGGCATDASPKNAAVEDGERPTLKKVSGRE
                     IIAELKQTVEETPKRDERATIETPPGVEKPSKETVEDLNVKAIEGPAVFEAVEKVYGE
                     GSVHHEGVIFIELQTKGAEQSAVWIGIKEPDERVQQLVDLLQPKVDAGEILAEAIYFF
                     RSPHTEKELYDLKEEVAAALKGLESERGSFDLYIDVITGAIEITHDFLKPDQQEELRK
                     QFADHTINITQNGRMIAEPGESTLISPEQTYTDTPVTEGGFVLAAGDGRIFVAGGTED
                     PVFYKFPEADKLKVGQRVNVEVSGKISKSYPGQGTAKFVEVLPDYRPANAKLSESQAV
                     AKALDIAGEKLSNGFVLIEEVTFDENEKTWVFKLWPDDGEGPVEVEDQ"
     gene            2334..3497
                     /locus_tag="VFX34_03260"
     CDS             2334..3497
                     /locus_tag="VFX34_03260"
                     /inference="COORDINATES: protein motif:HMM:NF022953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEX5563954.1"
                     /translation="MKKILSTLFITALLLGGCGTGESPRHGAVEVSKTLKKVSGREII
                     AELKQTVKESPERDKAAVAQTESHEYKEGLFGEPTPELAEDMAVQAIEGPIAIEAMMK
                     VYGGHESFENEGVLFFENQMSGAEQSGIWIGVKEPDERVQKLLDELQPKVDAGQILAE
                     PIYIFRSPHTQKELYELQNEVGRALKGMKSERGSYGLSVNTITGIIEIDHDFLKPAQQ
                     KELENMFPDRTFQFEQEGRMVAEPGESSIIYPEQTFTDTPVSEGGFILSAGEGKIFVA
                     GGTEGEVYYHFPEADKLNVGQRVKVEASGGIMESYPAQGGAKFVEVLPDYKPAGAVLS
                     ESQAVAKAIEMTDGNTAFGFLVIQEISFDEKEKKWVFKIREDDKEATLEVADQ"
     gene            3967..5223
                     /locus_tag="VFX34_03265"
     CDS             3967..5223
                     /locus_tag="VFX34_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX5563955.1"
                     /translation="MSKRLVSAIREHAFPLNDKALDTVLDAIGDARIVLLGEATHGTS
                     EFYKIRAALSKRLIQEKGFTIIAVEGDWPSAQAVNRYVKGFDKDGKSARQVLAESFNR
                     WPTWMWANEEVEELVEWLKRENAGKDAAGKTGFYGIDMYSLYESIDELLRFLQENPQF
                     KADLELAKKAFSCFEPYNRMPEHYALSTAHFTDECIREVTNLLTTIRSHEDLYSNEQE
                     KDLNVRMNALVAKNAESYYRAMMQDDAISWNIRDMHMVEAIGEILNYHGDDAKIIIWE
                     HNTHIGDASETTMADHDMINVGQLVREKFGREDTFAIGFGTYEGTVIAGESWGEPFQK
                     MNVPPAKFNSWEGQMHAAAAEDQVVLFTEENRHLFNEWIGHRAIGVVYNPEFEAYGNY
                     VSSRVGSRYDGFIFVDQTNALKPFSK"
     gene            complement(5255..5620)
                     /locus_tag="VFX34_03270"
     CDS             complement(5255..5620)
                     /locus_tag="VFX34_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5563956.1"
                     /translation="MADLAALMAWTFTEEVPVPEEIKDLLIDGEVAEVAYKTIRDVAV
                     VTNKRFIIADKQGLTGKKVEIYTIPFKSINMYSTENAGRIDFNAEIELWTKAGKLKLN
                     LNKKVDVRKLDKIIASAIL"
BASE COUNT         1589 a         1167 c         1545 g         1444 t
ORIGIN      
        1 cttcatggaa acggatatac agcttcagca atgtcttgat tggctcttga cggacatcct
       61 cctcgttgcc ccatttccgg tacgcagtga ggagcttccc gaattgtgtt ccccaatcac
      121 cgatgtaatt gattttcacc ggctgatagc catttttctc gagcaataaa gcgatggaat
      181 taccgatgac tgtggaacgt aggtgcccca tcgaaaacgg cttcgcaatg ttgggtgagg
      241 ataaatcgat cgtcaccact ccaccgttgc cttcattgga tgaaccgtat gtggcggatt
      301 tgtccacaat attttgcagg agatcagcgg caatcgcagt acggttcagg aaaatattga
      361 cgtatccatt gacagcttcc gcttttcgga tggatggatg tgataatgaa cccgcgattt
      421 cctccgcaat ggcgaccggc gatttccgca agatttttgc aaacggaaaa caaggcaatg
      481 caaaatcgcc taattttgaa ttggctgggc gttccagcat atgctcctcc aattggactg
      541 ggctgactga ctgtaaagca tgcaaaatat cttttttcat gtacacgaac tcctttttag
      601 gaaaaataaa aagccttcaa tctcaagaaa tgagacgaaa gcttgttccg cggtaccact
      661 caaattgccc ctgtcatgta tgggtagagg cccctttctc ctaacggcga gacaccggct
      721 tctcctactg cattcagaaa agcatctccg aagtgcgctt catgaattca ccccgccagg
      781 ctcacaccaa ccccggctcg ctacggaagt aaagctcatt actctcttca tcatcgatat
      841 tgtagttgaa tgtatcaaat tggagactgg gtgtcaatgg gtgaattgtc gaaactttta
      901 cgcgacttat tcgtctaact taataagtgg gaggtgtcgg aatgaaaaat atcttatcaa
      961 ctctgtttgt cgctgcttta ttgttgggag gatgtgcaac ggatgcatcg ccgaaaaatg
     1021 ctgcggttga agatggcgag cggccaacgt tgaaaaaagt gagtgggcgc gaaatcattg
     1081 ccgagcttaa gcaaaccgtt gaagagacgc ctaaacggga cgagagggcg actatagaaa
     1141 cgccccctgg ggttgagaag ccttcgaaag aaacggtgga ggatttgaat gtcaaagcaa
     1201 ttgaagggcc agctgtcttc gaggcggtgg aaaaagttta cggtgagggt agcgttcatc
     1261 atgaaggcgt catcttcatc gaacttcaga cgaagggtgc tgagcagtcc gccgtgtgga
     1321 tcggaatcaa ggagcccgat gaacgtgttc aacaattagt tgatctattg cagccaaaag
     1381 tggatgcagg cgaaatccta gcggaagcga tttatttctt ccgtagccct catacggaaa
     1441 aggaactgta tgacctgaag gaggaagtcg cggcagcgtt gaaagggttg gaatcggaac
     1501 ggggatcatt cgatctgtac atcgatgtca tcacaggcgc tattgaaatc acccatgatt
     1561 tcctgaaacc ggaccagcaa gaggaactgc gaaagcagtt cgccgaccat acgatcaata
     1621 tcactcagaa tggaaggatg atcgcggagc cgggggaatc aacccttatt tctcctgaac
     1681 aaacatacac ggatactcct gtgactgaag gtggattcgt attggccgcc ggcgatggaa
     1741 gaatttttgt agcaggcgga acggaagacc ctgtgtttta caaattcccg gaagccgaca
     1801 agctgaaagt cggccagcga gtgaatgtgg aagtatcagg aaagatctcg aaatcgtatc
     1861 ctggacaagg aactgcaaag tttgtggaag ttttgccgga ctataggccg gcgaatgcaa
     1921 agctttccga gtcacaagct gtggcgaaag cattggatat agccggtgag aaattgtcga
     1981 atggctttgt tctgatcgag gaagttacgt ttgatgaaaa tgaaaagacg tgggtattca
     2041 agctttggcc ggatgatggg gaagggccgg tggaagttga ggatcaatag aagttttagc
     2101 aaccgtcact tgttgacggt tgttttttat ggggaaattg gtcgaacggt gataaagctt
     2161 gcttgaccgg tgataaaccc cgactgagtg gtgataacgg ttgtctaagc ggtgataaac
     2221 tctctctgag tggtgataaa gtgtgtctga acggtgataa actattttgg aacaaaaaat
     2281 gcgtaacgtt aacttggatt gcccgtctaa tttattgact gggaggtttt ggaatgaaaa
     2341 aaatcttatc aactctattt attactgctt tattgttagg cggatgcgga acaggtgaat
     2401 ccccgcgaca tggcgcggtc gaagttagca agacgttgaa aaaagtgagt ggacgtgaaa
     2461 tcattgctga attgaagcaa acagttaaag aatcgcccga acgggacaag gcagctgttg
     2521 cacaaacgga aagccatgaa tacaaagagg gattattcgg agaaccgact ccggaattgg
     2581 cggaagacat ggcggtgcag gcgattgagg gaccgatcgc aatagaggcg atgatgaagg
     2641 tttatggcgg ccatgaaagc ttcgaaaacg aaggcgtcct cttttttgaa aatcaaatga
     2701 gtggtgcgga gcaatccggc atttggatcg gtgtgaagga gccggatgag cgcgttcaaa
     2761 agctgcttga tgagctgcaa ccgaaagtgg atgcaggcca aatcctggcg gaaccgatct
     2821 atattttccg tagcccgcat acacaaaagg aactctatga actacagaat gaagtcgggc
     2881 gagcgctgaa agggatgaaa tctgaacggg gatcatatgg attatcggta aatacaatta
     2941 caggaattat tgaaatcgac catgacttcc tgaaaccggc gcagcaaaag gagcttgaga
     3001 atatgttccc ggacaggaca ttccaattcg aacaagaagg caggatggtt gctgagcctg
     3061 gagaatcgag cattatctat cctgagcaaa cattcacgga tactcctgtt tcggaaggcg
     3121 gtttcatctt gtccgctggt gaagggaaga tctttgttgc gggcggaaca gaaggtgagg
     3181 tctattatca cttccctgag gcagacaagt tgaacgtcgg gcaacgtgtc aaagtcgagg
     3241 cgtccggtgg catcatggaa tcctatccgg cacagggtgg tgcaaagttt gtagaggtgt
     3301 tgccggatta taagccggct ggtgctgtgc tttccgaatc gcaggctgtt gcgaaggcaa
     3361 tcgaaatgac cgatgggaat acagcatttg gcttcttggt tattcaagaa atttcttttg
     3421 atgagaaaga aaagaagtgg gtctttaaga taagggaaga tgacaaggaa gcaacgctgg
     3481 aagttgcaga tcaataaaat ctgaggcaac cgtcacttgt ggcggttgtt tttatgggaa
     3541 aaagtggatg aacggtgata aagactgctt gagcggtgat aaacctcggc tgagcggtga
     3601 taacggttgt ctaagcggtg ataaactccc tttaagcggt gataaagtgt gtacgaacgg
     3661 tgataaacgt ttttgacact gaaaaccgca ttgagaagcg cggaaattca ttctaaaacc
     3721 cctcgaattg tagtcaatcg gtgataaatt cactagagtc ggtggtaaag tgctccgagt
     3781 cggtggtaaa cgcctgagag tcggtggtaa agtgctccga gtcggtggta aacaccggag
     3841 aatcggtcgt aaacacgcct aagtcggtgg taaccatttt cctgctttga aaatcacttt
     3901 gaattcccct cccccggttt ccgtccgacc cccatgggta tgctatggat gaaggagggg
     3961 attgatttgt cgaaaagatt agtgtctgcg attcgggagc atgctttccc gttgaatgat
     4021 aaagcgcttg atacagtatt ggacgcaatt ggggatgccc ggattgtcct gctaggggaa
     4081 gcgacgcatg gaacctcgga attctacaaa atccgtgccg ccttatcaaa acggctgatt
     4141 caggaaaaag ggttcacgat cattgcagtg gagggcgatt ggccatccgc ccaggcggtc
     4201 aaccgatatg tgaaagggtt tgataaagat gggaaatctg ctcggcaagt gttggcggag
     4261 tccttcaatc gctggccaac gtggatgtgg gcgaatgaag aagtcgagga gcttgtcgaa
     4321 tggctgaaac gtgaaaatgc tggaaaggat gcagccggta agacgggatt ttacggcatt
     4381 gatatgtata gcctttatga atcgatcgat gaacttctgc gatttctcca ggagaatccc
     4441 cagtttaagg cagatttgga gttggcgaaa aaggcattct cctgttttga gccatataac
     4501 cggatgccgg agcattatgc gctgtcgact gcccatttca cggatgaatg cattcgcgaa
     4561 gtgacgaact tgctgacgac gatccgcagt catgaagatc tttattcaaa cgagcaggag
     4621 aaggacttga atgtgagaat gaatgcattg gtagcgaaaa acgcggaatc gtattaccgc
     4681 gcgatgatgc aggatgacgc aatttcgtgg aacatccgcg atatgcatat ggtggaggcg
     4741 attggtgaga ttttgaacta ccatggcgac gacgcgaaaa tcattatctg ggagcataat
     4801 acccatatcg gcgatgcgtc ggaaacaacg atggctgacc acgacatgat caatgtcggt
     4861 cagctcgtcc gggagaagtt tggcagggaa gacacgtttg ccatcggctt tggaacatac
     4921 gaagggaccg tcattgctgg cgaaagctgg ggagagccgt ttcaaaagat gaatgtgcct
     4981 cccgctaagt tcaattcatg ggaagggcag atgcatgctg cggcagccga ggatcaagtg
     5041 gtccttttca cggaggaaaa ccgtcattta ttcaacgaat ggatcggcca tcgcgccatc
     5101 ggggtcgttt ataatccgga gtttgaagct tatgggaatt atgtgtcatc gagagtcggc
     5161 agccgatatg atggatttat ttttgtggat caaacaaatg cattgaagcc attcagcaaa
     5221 taggagccat gcatcgattg cacggctctt tccttcaaag tatggcactg gcgatgattt
     5281 tatcaagttt gcggacatcc acttttttat tcaagttcaa tttgagtttt cctgccttcg
     5341 tccataattc gatttctgcg ttgaaatcga tccgacccgc attttcagtg gaatacatat
     5401 tgatcgactt gaatgggatt gtgtagattt ccaccttttt acccgtaagt ccttgcttgt
     5461 ccgcgatgat gaatcgttta ttcgtaacga ctgctacgtc acgaatagtt ttatacgcaa
     5521 cctctgccac ttcaccgtca atgagcaaat ccttaatctc ctcagggact ggaacttcct
     5581 ccgtaaatgt ccaagccatc agtgcagcta aatcagccat tggaaaccct ccttcttttt
     5641 tatatttatc atcacatatg cgagtaagga agtatagatg ataattgaat tcttgaaaaa
     5701 tatctttcta cctatttacg atttaaacac aacatgttag ggtaa
//
LOCUS       DASULI010000150         4379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1933266,
            whole genome shotgun sequence.
ACCESSION   DASULI010000150 DASULI010000000
VERSION     DASULI010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933266"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFX34_03275"
     CDS             <1..297
                     /locus_tag="VFX34_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5563957.1"
                     /translation="DSEQLYEKPLHPYTQALLSAVPVPDPDFQKEQIIIEGDIPNPAD
                     PPTGCTFHTRCPFKMDICTKVAPHLSEQTTGHSVACHLYASQVDNDIKQLEGSL"
     gene            294..1886
                     /locus_tag="VFX34_03280"
     CDS             294..1886
                     /locus_tag="VFX34_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5563958.1"
                     /translation="MNKKRMWSAALLLMLVLSTVLAACSGGGKDSSGSSKDTLVFGRG
                     GDSTSLDPSRVTEGETFKVTVNLFETLLNFGEQDTTIQPGLAKEWETSEDGLTYTFKL
                     EEGVKFHDDTDFNAEAVVKNFERWANGDADKFPYYNSMFGGFKGDEGHVIDSVTADGD
                     YTVIIKLKRPQAPFLKNIAMSMFAIASPTAFEKGDDQFERNPVGTGPFKFVDWKTNDS
                     ITIEKFDGYWQKGLPKLNKIIFRSIPDNSARLNALIAGEIDLADGINPADGEKIEGNT
                     DLQLIERPSMNVGYLGLTVTRPPFDKKEVRQAMNHAIDKQTIIDSFFEGRADLAKNPM
                     PSSISGYNDDIEGYDYDPEKAKELLAKAGYPDGFEMELWAMPVPRPYMPDGAKVAEVI
                     QSNLADIGVKAKIVSYEWGTYLDKAAKGEADAFMLGWTGDNGDADNFLYVLLDEDNIG
                     SNNYTYFKNDEMHDLFIEAQTEVDEDKRIELYKKAQEIIHEEAPWVPLAHSTPLLAAA
                     KDLTGFVPHPTGSDLLSNVEFK"
     gene            2033..3037
                     /locus_tag="VFX34_03285"
     CDS             2033..3037
                     /locus_tag="VFX34_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563959.1"
                     /translation="MLNYTGRRLLQLIPVLLGMVFIVFMMIRAIPGNPAQIILGQQAT
                     PEAIAAMNARLGLDQPWYIQFFKYLGGILQGDLGESLRTRLPVADEIWPHLAATMELA
                     FFAILIAIIIGVNAGIVSAWFQNSWFDYIAMILALVGVSMPIFWLGLLGQWAFAIEFS
                     WLPTTGREQVRDPVNAITHLYIIDTIIQGRFDQLWQVIRHLMLPGIALATIPMAIIAR
                     MTRSSMLEVMRSDYIRTARAKGQKMFWVVYKHAFKNAVIPVLTIIGLQMGMLLGGAIL
                     TETIFAWPGIGRYIYDAINFRDYPVIQSGILVVAFIFVMINLIVDLLYGLIDSRIKYD
                     "
     gene            3055..3951
                     /gene="nikC"
                     /locus_tag="VFX34_03290"
     CDS             3055..3951
                     /gene="nikC"
                     /locus_tag="VFX34_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HEX5563960.1"
                     /translation="MSELTPKIDGIAEAEIERTTGPWREAWRSFRKSKVAVAGMIIIL
                     FFVLLAIFGPLIAKEGINEQMAADRLQPPSAEYWLGTDDFGRDILSRIIHGARISLSV
                     GFLSVIASVIVGSFLGIVAGYYGRWTDTIISRIFDIMLAFPSILLAIAIVSVLGPGLE
                     NALIAIAIINVPNFGRLIRSKVLSIKEDEYIMAAKAIGMKDARILFSHILPNSMAPVI
                     VQGTLAVATAILEAAALGFLGLGAQAPQPEWGKMLADSKSYLQSAPWTMIFPGLSIML
                     TVLGFNLMGDGLRDALDPKMKN"
     gene            complement(4163..>4379)
                     /locus_tag="VFX34_03295"
     CDS             complement(4163..>4379)
                     /locus_tag="VFX34_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEX5563961.1"
                     /translation="EFIQRQEVMDIFAKEIAITKEEEEFTAYHLLHVLSSLLNYEEQL
                     EYLDRLIVSNQRRHGHEVSNEMQNEIY"
BASE COUNT         1158 a          996 c         1081 g         1144 t
ORIGIN      
        1 gatagtgagc agctttacga aaagccgctg catccatata cacaagcgtt attatcggct
       61 gtacctgtgc cggacccgga ttttcagaag gaacagatca tcatagaagg agacatcccc
      121 aatccggcgg atccgccaac cgggtgcaca ttccatacac gatgcccatt caagatggac
      181 atttgcacaa aagttgcacc gcatctatcg gaacaaacta ccggtcattc tgtcgcctgt
      241 catctttatg catcccaggt cgacaatgat ataaaacaat tggaggggtc tctatgaata
      301 aaaagagaat gtggtcagct gcgttactac tcatgctagt tctatctact gtactcgctg
      361 cctgctcggg aggcggcaaa gatagcagtg gaagctcgaa agatacattg gtcttcggcc
      421 gcggcggcga ctccacatcg cttgatccgt caagggtgac ggaaggcgaa acattcaaag
      481 taacggtcaa tctatttgaa acactcttga atttcggtga acaagatacg actattcaac
      541 cgggactcgc gaaagaatgg gaaacaagtg aggatggatt aacgtacaca ttcaaattgg
      601 aagagggcgt caaattccac gacgacactg atttcaatgc tgaagcagtt gtaaagaact
      661 tcgagcgttg ggcaaatggc gatgcggata aattcccgta ttacaactcc atgttcggcg
      721 gattcaaagg agatgaaggc cacgtcatcg attcggtcac agctgatgga gattacacgg
      781 tcatcatcaa attgaaacgc ccgcaagcgc cgttcttgaa aaacattgcg atgagcatgt
      841 tcgcaatcgc aagtcctact gcgtttgaaa agggagacga ccaatttgaa agaaatccgg
      901 ttggtacagg tcctttcaaa tttgtagatt ggaaaacaaa tgattcaatt acaattgaaa
      961 aattcgatgg ctattggcaa aaaggattgc cgaaattgaa taaaatcatt ttccgttcca
     1021 tcccagacaa ctcagcacgt ttgaatgcac tcattgccgg ggaaatcgat ttagcggacg
     1081 gcatcaatcc tgcagatggt gaaaaaatcg aaggaaatac cgatctgcaa ttgatcgagc
     1141 ggccttccat gaacgtcggc tatctcggat tgacagtgac acgccctcct ttcgataaga
     1201 aagaagtacg ccaagcaatg aaccacgcaa tcgataagca aacaattatc gactcgtttt
     1261 ttgaaggaag agcagatctt gctaaaaacc cgatgccttc ttccatttca ggctataacg
     1321 acgacattga aggttacgat tatgacccgg aaaaagctaa agaactttta gcaaaagcag
     1381 gatatccgga cggctttgaa atggaattat gggcaatgcc tgttccgcgt ccgtatatgc
     1441 cggatggcgc aaaagtagct gaagtgattc aaagcaatct ggcggatatc ggcgtgaaag
     1501 ctaaaattgt aagctacgaa tggggaacgt atttggataa ggcggccaaa ggggaagcgg
     1561 atgctttcat gctaggctgg actggagata atggagacgc agataacttc ctctatgttc
     1621 ttttggatga agataatatc ggcagcaaca actatacgta ttttaaaaac gatgaaatgc
     1681 atgatctttt catagaagca caaacggaag tggacgagga taaacgtatc gaactttata
     1741 agaaagcaca ggaaatcatc catgaagagg cgccatgggt accacttgcg cactctacgc
     1801 cgcttctcgc agcagctaaa gatttgacag gattcgttcc acatccgaca ggttcagatc
     1861 tattatcgaa cgtagaattc aaatgatgga tccgcaattc gcttaacgct gatcggtaag
     1921 ggggagtcgt tcgatctccc ccttttccta tttgctaagt cttccacttt agtaccgtct
     1981 agactccggg tggcacccgc caccctccac ttttgtttgt gaggtgaagg tgatgcttaa
     2041 ttacaccggc agaagattat tacaactgat acccgtatta ctcggcatgg tcttcattgt
     2101 tttcatgatg atccgggcaa tacctgggaa tccggcccag atcattttag ggcagcaggc
     2161 gacacccgag gcaatcgcag cgatgaacgc gagacttggg ttggaccaac cgtggtatat
     2221 tcaattcttc aagtatttag gtgggatttt acaaggcgac ctcggagaat cgctgcgcac
     2281 gcgacttcct gtcgccgacg aaatatggcc tcatctggcc gcaacgatgg agctcgcttt
     2341 ttttgcaatt ttaattgcaa ttatcatagg tgttaatgca ggcatcgtct cggcttggtt
     2401 ccaaaattca tggttcgatt acatagcgat gattttggcg ctcgttggcg tttccatgcc
     2461 gattttttgg ttagggcttc tagggcaatg ggcatttgcc atcgaattca gctggctgcc
     2521 tacaaccggg cgggagcagg tgcgggatcc ggtcaatgcg atcacccatc tgtacatcat
     2581 tgacacgatc atccaaggcc gatttgacca attatggcaa gtgattcgcc atctcatgtt
     2641 gccgggcatt gcattggcga caattccgat ggcgatcatc gctcggatga cgcgttcaag
     2701 tatgcttgaa gtgatgcgtt ccgattacat ccggactgcc cgggcgaagg ggcagaaaat
     2761 gttttgggtc gtttacaagc atgcgttcaa aaatgcagtt attcctgtat tgacgatcat
     2821 cggtcttcaa atggggatgc tgctcggcgg cgcaatttta acggaaacga tttttgcatg
     2881 gccgggcatc ggacggtata tatacgatgc catcaacttc cgggattacc ccgtcatcca
     2941 atccgggatt ctcgtcgtcg ccttcatctt cgtcatgatc aacctgattg tggacttgct
     3001 ttatggtctg atcgattcga ggattaaata tgactgagga agggggaatg cggaatgtct
     3061 gaattaacgc ctaagataga tggaatagca gaagcggaaa ttgaaaggac gaccggaccg
     3121 tggcgggagg cttggcgaag cttcagaaag agcaaagtgg cggttgccgg aatgatcatc
     3181 atcctttttt tcgtgcttct tgccattttc ggcccgttaa ttgcgaagga aggaatcaat
     3241 gagcagatgg ccgcggaccg gcttcaaccg ccttcagccg aatattggct cggaacggat
     3301 gatttcggaa gggacattct ctctcgtatt attcacggtg cccggatctc gctttctgtc
     3361 ggattcctat cggtcatcgc ctcggttatt gtcggcagtt tccttggtat cgttgccggt
     3421 tattatggcc gttggacaga tacgatcata tccaggatct ttgatatcat gcttgcattt
     3481 ccctcgatcc tgttggcgat cgcaatcgtt tccgttttag gacctgggct tgaaaacgcc
     3541 ttgattgcca tcgcaatcat aaacgttcca aatttcggac gtctgataag atccaaagtg
     3601 ctgagcatca aggaagatga atatattatg gcggcgaagg cgatcggaat gaaagatgcc
     3661 cgtattttat tctcccacat ccttccgaat tcaatggcac ccgtcatcgt gcaggggacg
     3721 ctagccgttg caaccgctat tctggaagcg gcggcgctcg gctttcttgg actcggagca
     3781 caggcacctc agcctgaatg gggtaaaatg ctcgccgact cgaagtcgta tttacagtcc
     3841 gcaccatgga cgatgatttt ccctgggctt tccatcatgc tgacagtatt agggttcaac
     3901 ttgatgggcg acggtttaag ggatgcgctc gacccgaaaa tgaaaaacta actgaaaaaa
     3961 ccatcccaac ctgttggcaa acgctcgcat ccgttgttgc tcgtgtcgct cgtccgctca
     4021 tgaactttag ttctattcgc gtcttcgctc cactttgcgc ctaggatgac aaacgttttc
     4081 aatcaggttg ggggatttcc tgcgtatcat agatttcact aaaaaaacca tccttggaaa
     4141 atagcccaag gatggttttg cgttaataga tttcgttttg catttcattg ctgacttcgt
     4201 gtccgtgcct gcgctgattg gagacgatca gccggtccaa gtactccaac tgttcttcat
     4261 agttcaataa tgaagaaagg acatggagga gatgataggc tgtgaattct tcttcttcct
     4321 ttgtgatggc gatttctttc gcgaaaatat ccatcacttc ttgccgttgg atgaattcc
//
LOCUS       DASULI010000151         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_363875, whole
            genome shotgun sequence.
ACCESSION   DASULI010000151 DASULI010000000
VERSION     DASULI010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363875"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFX34_03300"
     CDS             <1..333
                     /locus_tag="VFX34_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563962.1"
                     /translation="LLFRQDKISTVFDYDIAGDEVLINECIGVAVFLAWHHDFNGNET
                     PEERYQAYIAAYEAERPLANDEMEMIPHLFAIIRAFRYDRIEEGIEKIKKGEGKPFLD
                     ETIVLLRG"
     gene            348..1001
                     /locus_tag="VFX34_03305"
     CDS             348..1001
                     /locus_tag="VFX34_03305"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5563963.1"
                     /translation="MFRIPESIAELGRERIKGFPVEGFVYGEGPQKPELMLIGEAPGE
                     FEAIDGIPFIGRAGKELMKSLDSIGLTREDVYITSTVRSRPYKWGEKRERDGTMTKRK
                     YNRPPTGKEILAHAPVLDYEIANIEPKLIVALGNVGLQRLLGKDAKVTELHGQLLVRP
                     VRFLPDLESTTFEWTDKAYRIVPTFHPAAVFYRPSHRPDLDADWLKIGQFLEDLRNE"
     gene            994..1524
                     /locus_tag="VFX34_03310"
     CDS             994..1524
                     /locus_tag="VFX34_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015843942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEX5563964.1"
                     /translation="MNERSYKRILIIGCSGAGKSTLSVELSRRLQLPVLHLDALFWNE
                     GWVPTPKPEFREKLQSELEKPAWIIDGNFNSTIEMRAQYADLIIFLDFPNWLCLSRVF
                     KRRWMYRGKTRPDMAEGCPEKVDWEFVKFIWTYPKKKRPGVLQMLERADAEVLILKSP
                     GEVENWLAVLNKGDIG"
     gene            1521..2096
                     /locus_tag="VFX34_03315"
     CDS             1521..2096
                     /locus_tag="VFX34_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563965.1"
                     /translation="MKKSLWSGLIFIVLVCFVTLFFPYSPFSFYQSVSVSTELPILQD
                     YKMKLYEFRKVYEENEGQKREEFYSKDEVGYHTEYILQTYEMEMMTTSRSSISKGDLN
                     IMFEDLRFLQDRLLDITFEEAHSSESRKYLDATLRWSRAAEEGILKIRSAPYYSRFHM
                     IYRLEEIQDEIRKSFDMYTEFYNAYYWYEGE"
BASE COUNT          695 a          442 c          652 g          581 t
ORIGIN      
        1 cttttattcc ggcaagataa aatctccacc gtctttgatt acgacattgc aggggatgaa
       61 gttctcatca atgagtgcat aggcgtcgct gttttcttag catggcatca cgacttcaat
      121 ggaaatgaaa cccctgaaga acgataccaa gcatacatag ccgcttatga agctgaaagg
      181 ccactagcaa acgacgaaat ggagatgatc ccccatctat tcgcaatcat ccgtgcattt
      241 cgatatgatc ggatagaaga aggcatcgag aagatcaaaa aaggtgaagg caaaccgttt
      301 ttagatgaaa caatagtgct gttgcgaggc tgaaagggtg tgtttcaatg tttcgaatac
      361 ctgaatcgat tgccgaactt ggcagggaac ggatcaaagg atttccagta gaagggtttg
      421 tctatgggga aggacctcag aagccggagc tgatgttgat tggcgaagcg cctggggaat
      481 tcgaggctat agacggcatc cctttcatcg gcagagccgg caaggaactg atgaagtcgc
      541 tcgattcgat cggattgacg cgagaagatg tctatatcac gagcacggtg cgcagcaggc
      601 cgtataaatg gggagaaaaa cgcgagcggg acggcacgat gacgaagcgc aaatacaatc
      661 gtcctccgac ggggaaagag atcttggcgc atgcgccggt acttgattat gaaatagcaa
      721 atatcgaacc gaagctgatt gtagcgctcg ggaatgtcgg gctacaaaga ctgcttggca
      781 aagacgcgaa agtgacggaa ctgcacggac agttgttggt gcggccggtc cgttttcttc
      841 ccgacctcga aagcacgacg ttcgaatgga cagataaggc ataccgcatc gtgccgacat
      901 tccatccggc cgccgtcttt taccggccat cgcataggcc ggatctggat gcggattggt
      961 tgaagattgg ccagttcttg gaggatttac ggaatgaatg aaagatcata taagcggatt
     1021 ctcatcatcg gatgcagcgg cgctggaaaa tcgacattat cggtcgagct atcgcgccga
     1081 ctgcaattgc ctgtcctgca tttggacgcc cttttttgga atgaaggatg ggtgccgaca
     1141 ccgaagccgg aatttcggga aaaacttcaa agtgaactgg aaaagcccgc atggatcatc
     1201 gatggaaatt tcaactccac gattgagatg cgggctcaat atgctgattt gatcatcttc
     1261 ctcgacttcc cgaattggct atgtttgtcg cgggtgttca agaggagatg gatgtatcgg
     1321 gggaaaacgc gtccagatat ggcggaggga tgtccggaga aagtcgattg ggagtttgtg
     1381 aagttcatat ggacgtatcc gaagaaaaag cggccgggcg ttttgcaaat gcttgaacgt
     1441 gccgacgcgg aagtattaat tttgaaatcg ccgggggaag tggagaattg gttagcggtt
     1501 ttaaacaagg gggatatagg gtgaagaaga gcttgtggag tggcttgatt tttatcgtac
     1561 tggtatgttt tgtgacgtta tttttcccgt attcgccttt cagcttctat caatccgttt
     1621 cagtcagtac ggaacttcct attttgcaag actacaaaat gaaattatac gaatttagga
     1681 aggtgtatga ggagaatgaa ggacagaaac gagaagagtt ttattcgaaa gatgaggtag
     1741 ggtatcatac tgaatacatt ctccaaacgt atgaaatgga gatgatgacg acttccagat
     1801 caagcatcag taaaggtgac ttgaatatta tgtttgaaga ccttagattc ttgcaggaca
     1861 ggttattgga tatcactttt gaggaggcac actcgtcgga atccagaaag tatctagatg
     1921 cgactctccg gtggagccga gcagctgagg aagggatttt gaagattaga agtgcccctt
     1981 attattcgag gttccatatg atctatcgtc ttgaagaaat tcaagatgaa attagaaaga
     2041 gctttgatat gtatacggaa ttttataatg cttattattg gtatgaggga gaataaaacg
     2101 tcgaaacgtg ttcatgcatg tttggacaag gagtatcgtg acgatagtat gagtgtactt
     2161 ttatgggtaa aataagtgtg tacggaaaaa gcatcggagg aaaaaatggc aactaagaaa
     2221 tcgctggcgg attaccaaag agaatatgag gcaatcatac atgcagacca accaagaaga
     2281 gatatgctcc tcgtcggatt gatgactgag atggagaagc aattcaagat ccctatgccg
     2341 agaaataaag agtgggaaag agagaacccc
//
LOCUS       DASULI010000152         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1290574,
            whole genome shotgun sequence.
ACCESSION   DASULI010000152 DASULI010000000
VERSION     DASULI010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290574"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /locus_tag="VFX34_03320"
     CDS             complement(<1..2138)
                     /locus_tag="VFX34_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEX5563966.1"
                     /translation="MKPVILAEKPSQAKAYADAFSVRKHEGFLEINPCPIFPAGAYIT
                     WGVGHLVELKEPHAYNPAWKRWSLGSLPILPDRYEFQVAKGKFKQFQIVKKLIRGTDT
                     VINACDVDREGSNIFYSIYYQTGARNQTIKRLWINSLEVDEVRKGFSNLRDNRKDLQM
                     YEEAKSRQISDWLVGMNASRLYTLLLKAKGVQEVFPIGRVQSPTVYLIYQRQREIETF
                     ISEPFFEVEATFKAEHGTYKGKAKAKEPKREIIQELLAKHGIQPDSPGVITSVTHTDK
                     RTPPPQLHSLSTLQATANRRWKMSPANVLKTMQGLYEKKLVTYPRTDARHITPNEFAY
                     LKDQVGDYQQLIGHPFPVASLAPKKRYVDSSKVQEHYAIIPTKKVPTQAVLGRMSPLE
                     RNLYEEVVRTTLAMFHTDYLYTETKVTTDVNGLPFFTTGKTERDLGWKALFQRSKDEK
                     EDPALPPLRMQETVQSDIGIKEGKTMPPKPYTEGQLIAMMKTCGKLVEDKEETDILKE
                     IEGLGTEATRSGIIETIKKHGYITVSKNIVSITEKGRVLCQAIEGNLLASPSMTAKWE
                     TYLRKIGNGEGSGQHFLDNISKFIAKLLDDVPKQLEAKPIDATKVPPRQSKTRGSYQA
                     VEVAPCPACQNGMILARKGFYGCSNYKNGCKQTLPGIFLKKKLTPAQVKLLCTKGKTN
                     TIKGFTANNGNKFDASLALENGKLNLVFQK"
BASE COUNT          488 a          594 c          516 g          633 t
ORIGIN      
        1 tatttttgaa ataccaaatt cagttttccg ttctccaaag caaggcttgc atcgaactta
       61 ttgccattat tggcggtgaa gcctttgatc gtattcgttt tgcctttcgt gcaaaggagt
      121 ttcacttgcg caggtgtcag cttcttcttc aggaagatgc ccggcaacgt ctgtttacag
      181 ccatttttat aattgctgca gccgtaaaat ccttttcggg ccaaaatcat gccattttga
      241 catgcagggc atggggcaac ctccacggct tgataagagc cgcgcgtttt cgattgccgc
      301 ggcggaacct tcgtcgcatc gatcggcttt gcttccaact gcttcggcac gtcatccaat
      361 agcttcgcga tgaacttcga tatattatcg aggaaatgct gaccgctgcc ttcgccattg
      421 ccgattttcc gcaaataggt ttcccacttc gcggtcatcg atggactggc aaggagattc
      481 ccttcaatcg cctggcaaag gacacgaccc ttctctgtaa tcgatacaat atttttcgac
      541 acggtaatat agccatgctt tttaattgtc tcgataatgc cacttcgggt cgcttccgtc
      601 ccaagtccct cgatttcttt caaaatatcc gtttcctctt tatcttcgac cagttttccg
      661 catgttttca tcatcgcaat gagctgacct tccgtgtacg gcttcggcgg catcgtcttg
      721 ccctctttaa tgccgatatc agactggaca gtctcttgca tgcgaagtgg cggcaaagca
      781 gggtcctctt tttcatcttt tgaccgttgg aataaagcct tccatcccaa gtccctttcc
      841 gttttgccag tcgtaaaaaa cggcaagccg ttcacatccg tcgtcacttt cgtctcggta
      901 tacaaataat cggtatggaa catggcgagc gttgtacgga caacctcttc atacagattc
      961 cgttcgagcg gcgacattct gccaagaacc gcttgtgtcg gcactttttt cgtcgggatg
     1021 attgcgtaat gctcctgcac cttcgaactg tcgacatatc gtttcttcgg ggcaagagaa
     1081 gcgacaggga aggggtgtcc gatcagctgt tggtaatcgc cgacctggtc cttcaagtac
     1141 gcaaattcat tcggcgtaat atggcgcgca tccgtccgcg gataggtgac aagttttttc
     1201 tcatataagc cttgcatcgt cttcaacacg ttcgcagggc tcatcttcca acgtcggttc
     1261 gccgtcgcct gcaaagttga cagggaatgg agctgcggtg gcggcgtccg cttatcggta
     1321 tgcgtgacag aggtgattac gccaggcgaa tccggttgga ttccgtgttt tgcaaggagt
     1381 tcctgaatga tctcccgttt cggctccttc gctttcgcct tccccttata cgtaccatgt
     1441 tccgctttga acgtcgcttc cacttcgaaa aacggttcag agataaatgt ttcaatctcg
     1501 cgctgccgtt gatagatcaa gtacactgta ggcgactgca cacgtccgat tggaaacact
     1561 tcctggaccc ctttcgcttt caataggagt gtatacaatc gggaagcgtt catcccgacg
     1621 agccagtcgc tgatctgacg ggatttcgcc tcttcataca tctgcaaatc cttgcgattg
     1681 tcccgtaagt tcgaaaaccc tttccgcact tcatccactt ctagcgaatt gatccaaagc
     1741 cgcttgatcg tctgattgcg ggcgcccgtt tgataataaa tgctatagaa gatgttcgat
     1801 ccttcccggt ctacgtcaca agcattgatg accgtatccg tcccgcgaat gagcttcttc
     1861 acaatctgaa actgcttgaa cttccccttc gccacttgaa actcgtacct gtcggggagg
     1921 atcggaagac tcccgagcga ccaacgtttc caagcggggt tgtaagcgtg cggctccttc
     1981 aactcgacca agtggccgac gccccatgtg atgtaagctc ctgctgggaa aatcggacac
     2041 gggttgatct ccaagaaccc ttcatgcttc cgcacggaaa aagcgtctgc atacgctttc
     2101 gcttgggaag gtttttcggc taggataacg ggtttcatag gaacacctcc aatcagagta
     2161 catttgttct attagtatct cacaaagagg agaagattgc tatggagttg aggatggtca
     2221 ccttttctaa a
//
LOCUS       DASULI010000153         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_642425, whole
            genome shotgun sequence.
ACCESSION   DASULI010000153 DASULI010000000
VERSION     DASULI010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642425"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFX34_03325"
     CDS             <1..485
                     /locus_tag="VFX34_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5563967.1"
                     /translation="IAMMNGYAFGGGMELALACDFRFAADSAVMGLTETGLAIIPGAG
                     GTQRLPRLIGEAKAMELILTARRLKADDAHMCGMLTGVAPANILQETVDRFVEELLSN
                     GPVAVQQAKFAIKQGMNADLQTGLAIERKAYELTIPTEDRLEALAAFAEKRKPNFKGK
                     "
     gene            complement(622..1029)
                     /locus_tag="VFX34_03330"
     CDS             complement(622..1029)
                     /locus_tag="VFX34_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEX5563968.1"
                     /translation="MTTVDIEKAIQIYKEASASTLSMMEYVLLLLNELQKNIQQCKQA
                     IEAGDTDERDRTLQKAQDFLFELMTTTDHEAERSDRLMTIYLHMNQCLVQTHMTKTNE
                     LLAHLEEMTVQLIASWQVAKQVTRRRTFTGDQL"
     gene            1144..1956
                     /locus_tag="VFX34_03335"
     CDS             1144..1956
                     /locus_tag="VFX34_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX5563969.1"
                     /translation="MRILSSEHIAVSANRSMRNAGQIEKSLLKLGTGTKIAKGSDNAS
                     GLSISETMRAQIRGISRAQSNMQDGLSVLEASNEGLNNVNGLLQRARELAVMSANDTL
                     TVNDRAASQVELDHLLGAVDDTATKLEFNTKKILGQNGSLTLQVGANPGQQMQITLVD
                     VSSKALGLENASLELRDLADKLISKIDEAIKTVSGHLTRIGSNMEAVEHHLTNALTFE
                     NNLTKSLSLLEDTDMAKEMMNFVNLDIRQQGDHLLVKQVNQNIHSVMSLFSK"
     gene            complement(1981..2265)
                     /locus_tag="VFX34_03340"
     CDS             complement(1981..2265)
                     /locus_tag="VFX34_03340"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HEX5563970.1"
                     /translation="MPVYNVMEEVVLGVLQQYENELHLTCNCDRCKNDIMAIALNEIK
                     PRYIVNDKLSPVIRAEHVADRQGATNILSTVVKAARIVTANPRCENAKKE"
     gene            complement(2435..>2618)
                     /locus_tag="VFX34_03345"
     CDS             complement(2435..>2618)
                     /locus_tag="VFX34_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5563971.1"
                     /translation="RKMSVTELTEKVGITMANLSILKNGKAKAIRFSTLEAICKALDC
                     QPGDILEYQEDEDAEA"
BASE COUNT          570 a          539 c          771 g          738 t
ORIGIN      
        1 cgattgcgat gatgaacggc tatgcattcg gcggcgggat ggaacttgct ttagcttgcg
       61 atttcagatt tgccgcggat tcggcagtaa tggggctcac ggagacgggt cttgcaatca
      121 tcccgggggc tggagggacg cagcggctgc cgaggctgat cggcgaagcg aaggcgatgg
      181 agcttatttt aacggcaaga cggttgaagg cggatgacgc gcatatgtgt ggcatgttga
      241 cgggcgtcgc tcccgcgaat attttgcagg agacggtgga ccgttttgtc gaggagttgc
      301 tctcaaacgg accggttgcg gtgcagcaag cgaaatttgc gatcaagcaa ggcatgaacg
      361 ccgatctgca gacggggctt gccattgagc ggaaagcgta cgagttgacg atcccgacgg
      421 aagatcggtt ggaggcactg gcggcttttg cggagaaacg gaaaccgaat tttaaaggaa
      481 aatgatatta gagcgtcctg gggttagcgg gttttgctaa ttcccagggc gttttcgttt
      541 atgtgaatga gttctcgttc tttatcggaa agttctcgtt tacgtgaaaa aagttctcgt
      601 ccgctaccaa aacgtttccg ttcatagttg gtcccctgtg aaagtcctgc ggcgcgtcac
      661 ttgtttggcg acttgccatg acgcgattaa ctgaacggtc atttcctcca gatgagcaag
      721 cagctcgttc gttttagtca tatgcgtctg gacgaggcat tggttcatgt ggaggtagat
      781 cgtcatgagc cggtcgctcc gttcggcttc atggtcggtc gttgtcataa gttcgaagag
      841 gaagtcctgc gctttttgca atgtacggtc acgttcgtcg gtgtcccctg cctcgatcgc
      901 ctgcttgcat tgctggatgt ttttctggag ttcgttgagc agcagaagga cgtattccat
      961 catcgataat gtggaggcgc tcgcttcttt gtagatttgg atggcttttt cgatgtctac
     1021 ggttgtcaat gtgggtgcct cctgtcgctt tttcttttgt atcggctttt ttctgttcat
     1081 ttccatcgtt tttggccgat atatagatgg agataagatg atagttggaa ttggggtgat
     1141 tgaatgcgta ttttgagttc tgagcatatt gcggtttctg cgaaccggtc gatgcggaac
     1201 gcgggacaga ttgagaagag tttgttgaag ctcggaacag gaacgaagat tgcgaagggc
     1261 agcgacaatg cttcgggtct gtcgatttcg gagacgatgc gggcgcagat tcgcgggatt
     1321 tcacgggcac agagcaatat gcaggacggg ctgtctgttc ttgaggcgtc gaatgaaggg
     1381 ctgaataatg tgaacggcct gctgcagcgt gcacgtgagc ttgcggtgat gagtgcgaat
     1441 gatacgctga cagtgaatga ccgtgcggcg agccaggtcg agcttgacca tcttctgggt
     1501 gctgtcgatg atacggcgac gaagcttgag ttcaatacga agaagatttt agggcagaac
     1561 ggatcgttga cgctgcaggt cggggcaaat cccgggcagc agatgcagat tacgcttgtc
     1621 gatgtcagtt cgaaggcgct cggccttgaa aatgcttctt tggaattgcg ggaccttgcg
     1681 gataagttga tttcgaagat cgatgaggcc atcaagacgg tttccgggca tttgacgcgg
     1741 atcggctcga atatggaagc ggtcgaacat catttgacga atgcgttgac attcgagaat
     1801 aatttgacga agtcgctttc gttgcttgag gatacggata tggcgaagga aatgatgaat
     1861 ttcgtcaatc tggatatccg tcagcaaggg gatcatctgc ttgtgaagca ggtgaatcag
     1921 aacatccata gtgtgatgtc gttgttttcg aagtgagtag gcgaagggat ttctccctgc
     1981 ctactctttt tttgcgtttt cgcaacgcgg gtttgcggtg acgatgcggg cagctttcac
     2041 gacggtggag agaatatttg tggcgccttg ccggtcggcg acgtgttccg cgcggatgac
     2101 cggacttagt ttatcgttca cgatatagcg cggcttgatt tcgttgagcg cgatggccat
     2161 gatgtcattt ttgcatcggt cgcaattgca tgtaagatgg agttcgttct cgtattgttg
     2221 gagtacgccg aggacgactt cctccattac gttgtatact ggcatgtttc gtgaccccct
     2281 gatgtggagt gattctattt atcgtaccat ttctaggaaa agcctggaag tgtaggaaag
     2341 ttgtattgac acataaggac tttacaccta ttcgaaagga tgtggatgaa aaaagccgga
     2401 tccctaagga aacgactttt tatcactcac tttatcaagc ttcagcatct tcatcctctt
     2461 ggtattctaa aatatcgcca ggctgacaat ccaaagcctt gcaaatcgcc tctaacgttg
     2521 aaaatcggat ggcttttgcc tttccatttt tcaatataga gaggttcgcc attgtaattc
     2581 cgactttctc tgtaagttct gttacgctca ttttcctt
//
LOCUS       DASULI010000154         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1174760,
            whole genome shotgun sequence.
ACCESSION   DASULI010000154 DASULI010000000
VERSION     DASULI010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174760"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="purM"
                     /locus_tag="VFX34_03350"
     CDS             complement(<1..659)
                     /gene="purM"
                     /locus_tag="VFX34_03350"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5563972.1"
                     /translation="MSKAYEKAGVNIEAGYESVERMKSHVARTARKGVAGAFGGFGGM
                     FDLSELNYKEPVLISGTDGVGTKLKLAFLADKHDTIGIDCVAMCVNDIVAQGAEPLYF
                     LDYIALGKAVPEKVEAIVKGIADGCVQSGAALIGGETAEMPGLYDVEEYDLAGFAVGA
                     CEKSKVVTGEKVAEGDVLVGIASSGIHSNGFSLVRQIVLEEQGYKIDEYIAGFEELGK
                     VG"
     gene            complement(672..2099)
                     /gene="purF"
                     /locus_tag="VFX34_03355"
     CDS             complement(672..2099)
                     /gene="purF"
                     /locus_tag="VFX34_03355"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5563973.1"
                     /translation="MLAELRGLNEECGVFGIWGHEDAAQLSYYGLHALQHRGQEGAGI
                     VTKNGKGLHVVKGEGLVNDVFSGDALKTLTGRAGIGQVRYTTEEGRGAENVQPLVFRS
                     MTGSLAIAHNGNLVNAKDLKESLEKQGSIFQTTSDTEVLAHLIKRSSGGSLTQRDRVK
                     KALSMLKGAFAFVILTEDGLLVAQDPNGLRPLSLGKLGDAWVVASETCAFDLIGAETI
                     RSVEPGELVVINDKGLHSDRFAEPADRAICSMEYVYFSRPDSDIDGINIHMARKRCGK
                     QLAREVNIEADVVTGVPDSSISAAIGFSEESGIPYELGLIKNRYVGRTFIQPTQAMRE
                     RGVKMKLSPVHQVVEGKRVVMVDDSIVRGTTSRRIVKMLRDAGAKEVHVVIAAPPLVS
                     PCFYGVDISTDSELIATGRSIEEVRELIGADSLTFLSAEGMLKAIGRPSEMKNCGQCL
                     ACFTGEYPTEIYADTVLPHEKEIGR"
     gene            complement(2075..>3697)
                     /gene="purL"
                     /locus_tag="VFX34_03360"
     CDS             complement(2075..>3697)
                     /gene="purL"
                     /locus_tag="VFX34_03360"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX5563974.1"
                     /translation="ASGVGNTVLYVGAKTGRDGIHGATMSSAEVAVDKEEELPVMQAG
                     DPYLEKLLMEACLELVKSDALIGIQDMGAAGLTSSSAEMASKAGYGVELNLDLVPQRE
                     EGMTAYEMMLSESQERMLVVVKQGREQEIIDLCKKYGLEAVAIGKVTGDKMLRLTHKG
                     EVVAEILADTLAEDAPVYHKKSEEPAYFREFQAMDNEEPTVEDLKETLSALLQRPTIA
                     SKEWVYSQFDSQARTNTVVAPGSSAGVIRVRGTNKGLAMTADCNSRYIYLDPETGGKI
                     AVAEAARNLICSGAEPIAATDCLNFGSPDKPEVFWQIEKSADGIAEACRTLNAPIIGG
                     NVSMSNEVNGVPVYPTPTMGLVGLVHDLSQVTTTSFKQAGDAIYLIGEAATEFGGSEL
                     QQMVHGKIFGKVPAIDLEVEAKRQQALLKAIQSKLVQSATDLSEGGFAVALCEKAFDA
                     DGLGAEVTVTGSAVTALFSETQSRFLVTVKEELTTAFEAIVQDAVQIGTVTDGDNIVI
                     KGDDETVLIDGTVEEFRSAWKGAIPCLLNSEA"
BASE COUNT          810 a         1161 c          809 g          917 t
ORIGIN      
        1 ccgaccttgc cgagctcctc gaatcccgcg atatattcat caatcttata tccctgctct
       61 tccaagacga tttggcggac gagcgagaat ccgttcgagt ggatgccgct tgaagcgatg
      121 ccgacgagca cgtccccttc agcgactttt tcccctgtga cgactttgct cttttcacaa
      181 gcgccgaccg caaatccggc gaggtcatat tcttcaacat cgtagagccc cggcatttca
      241 gccgtctctc cgccgatcaa tgcagctccc gattgcacac acccgtccgc gattcctttg
      301 acgattgcct cgaccttttc agggaccgct ttcccgagag cgatataatc caagaaataa
      361 agcggttctg cgccttgcgc gacgatatcg ttcacacaca tcgctacaca gtcgatgccg
      421 atcgtatcat gcttatccgc aagaaacgcc aatttcaatt ttgtgccgac gccgtccgta
      481 ccagaaatga gcacaggctc tttgtaattc agctcggaaa gatcgaacat ccctccgaag
      541 ccgccgaatg ccccggccac gcctttacgt gccgtccgcg cgacatgcga tttcatccgt
      601 tcgaccgatt cgtaacccgc ctcgatattc actcccgctt tttcatacgc ctttgacatg
      661 gaagttcctc cttatcgccc gatttctttt tcgtgcggca atactgtatc cgcatagatt
      721 tctgtcgggt attcacccgt gaagcacgca agacattgac cgcaattttt catttcagat
      781 ggtctgccga ttgctttcag catcccttcc gccgacagga acgtcaacga atccgcgccg
      841 atcaattccc gcacttcctc aatagaacgc cctgtcgcaa tcaattccga atccgtactg
      901 atgtcgacgc cataaaagca aggactgacg agcggaggcg ccgcaatgac gacatgcact
      961 tctttcgcac ctgcgtccct cagcattttg acgatccttc tggaagtcgt cccacggaca
     1021 atcgaatcgt caaccatgac aacccgcttg ccctcgacga cttgatgcac cggcgaaagc
     1081 ttcatcttca cgcctcgttc acgcattgcc tgcgtcggtt ggatgaacgt cctgccgaca
     1141 tacctgtttt taatcaaccc taattcatac gggatgccgc tttcctcgga aaatccgatc
     1201 gcagccgaaa tgcttgaatc cggaacaccc gtgacgacat ccgcttcaat gttgacttcc
     1261 cttgccagtt gcttgccgca acgcttccgc gccatatgga tattaatgcc gtcgatgtcc
     1321 gaatccggtc tcgaaaaata gacatattcc atcgagcaga tcgccctgtc tgcaggctct
     1381 gcgaatcgat cggaatgcaa gcctttatcg ttgatgacga ccaattcgcc gggctcaacc
     1441 gaccggatcg tctctgcgcc gatcaagtcg aacgcgcatg tttcggatgc gacaacccat
     1501 gcatccccca atttcccgag cgacaatgga cgcaaaccat ttggatcttg tgcaacgagc
     1561 aaaccgtcct ccgtcaagat gacaaatgcg aaagcgcctt tcaacatcga cagcgccttt
     1621 ttcacacggt cacgctgcgt caatgaaccg ccgctgctcc gcttgatcaa atgggcaagc
     1681 acttcggtat cagacgttgt ctgaaaaata ctcccctgct tttcaagact ttcctttaaa
     1741 tccttcgcat tgaccaagtt gccgttatgc gcaatcgcca aactgcctgt catggaacgg
     1801 aagacgagcg gctgcacatt ttcagcgccc cgcccttcct ccgtcgtgta tcggacttgg
     1861 ccgatccccg cgcgccctgt caaggttttc agcgcatcac cggaaaacac atcattcaca
     1921 agaccttcgc ctttgacgac atgaagccct ttgccattct tcgttacaat ccctgcgcct
     1981 tcctgaccgc ggtgctgcag cgcatgcaag ccataatagc ttagctgcgc cgcatcttca
     2041 tgcccccaaa tgccgaacac gccgcattct tcgttcaggc ctctgagttc agcaagcatg
     2101 gaattgcccc tttccaagca gaacggaatt cttccaccgt cccgtcaatc aaaaccgttt
     2161 cgtcgtcgcc tttgatgacg atgttatctc catcggtaac cgtaccgatt tgcactgcgt
     2221 cttgaacgat cgcttcaaaa gcagtcgtca gctcctcttt caccgtcacg aggaagcggg
     2281 attgcgtttc actgaataaa gccgtcaccg ctgaacccgt tacagtcact tccgcgccaa
     2341 ggccatccgc atcgaatgcc ttctcgcaaa gcgcaacagc gaagccgcct tccgacaaat
     2401 ctgttgctga ctggacgagc ttgctttgga tcgctttcag aagcgcttgt tgacgtttag
     2461 cttccacttc caaatcgatc gctggcactt ttccgaagat cttgccatgg accatttgtt
     2521 gaagttcgct tccgccaaat tccgtcgcgg cttcgccgat caagtagatc gcgtcgcctg
     2581 cttgtttgaa tgaagtcgtc gtcacttgcg acagatcatg cacaagccct accaagccca
     2641 tcgtcggcgt cggatagacc ggaaccccgt tcacttcatt cgacatcgat acgtttccgc
     2701 cgatgatcgg cgcattcaat gtacggcatg cttccgcaat gccatccgct gatttttcga
     2761 tctgccagaa cacttccggc ttgtcggggc tgccgaagtt caggcaatcg gttgcagcaa
     2821 tcggctctgc accagaacag atcaggttgc gggcagcttc agcgactgca atctttccgc
     2881 ccgtttccgg gtcgaggtat atatagcggg aattgcaatc cgccgtcatc gcaaggcctt
     2941 tatttgtacc gcgaacacgg atgaccccag ctgaagaacc tggcgccaca accgtattcg
     3001 tgcgcgcctg cgaatcgaat tggctgtaca cccattcttt tgacgcgatt gtcgggcgtt
     3061 gcaataaagc ggataacgtc tctttcaaat cttcgacagt cggctcttca ttgtccatcg
     3121 cctggaactc cctgaagtag gcaggctctt ccgatttctt atggtagacg ggcgcgtctt
     3181 ccgcaagcgt atctgctaga atttccgcga caacctcacc tttatgtgta agccgaagca
     3241 ttttgtcacc cgttactttc ccgattgcca ctgcttccag cccatatttc ttgcacaagt
     3301 cgatgatttc ctgctcacgc ccttgcttca cgacgacgag catacgctcc tgcgattcgg
     3361 aaagcatcat ttcataagct gtcatgcctt cttcgcgctg cggaacgaga tccaagttca
     3421 actcaacgcc gtagcccgct ttcgaagcca tttccgctga tgaagaagta agtcctgcag
     3481 cacccatgtc ctggattccg attaacgcat cggacttcac caattcaagg caagcttcca
     3541 taagcagctt ttcaagatac ggatcacccg cctgcatgac tggcagttct tcctctttat
     3601 cgaccgccac ttccgcagaa gacatcgtcg caccgtggat gccgtctcgc cccgtttttg
     3661 caccgacgta cagcactgta ttgccgacac ctgatgc
//
LOCUS       DASULI010000155         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_943236, whole
            genome shotgun sequence.
ACCESSION   DASULI010000155 DASULI010000000
VERSION     DASULI010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943236"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VFX34_03365"
     CDS             complement(<1..176)
                     /locus_tag="VFX34_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2759 family protein"
                     /protein_id="HEX5563975.1"
                     /translation="MNILMVIFGLVAIFAVIGTVQAFKERNVLSILFNVAAAVIFGGF
                     TIATIVFQGYPPALV"
     gene            337..972
                     /locus_tag="VFX34_03370"
     CDS             337..972
                     /locus_tag="VFX34_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5563976.1"
                     /translation="MLEIRTYPLGPIQTNCYVIYNREGECLVIDPGEEGDRIIAEIEK
                     MNGKPLAILLTHAHFDHIGAVDRVRNHFDVPVHIHEAEKNWLGNPDLNGSSRYPGLPH
                     VKNKAADQFLQEGELQIGSFRLEVRHTPGHSPGSVSFIFKDAQMAVVGDTLFRGSVGR
                     TDLPGGDTKTLLQSIHEKLLILDDDFIVYPGHGPATTPEEEKDMNPFLNGF"
     gene            complement(1018..1260)
                     /locus_tag="VFX34_03375"
     CDS             complement(1018..1260)
                     /locus_tag="VFX34_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2626 family protein"
                     /protein_id="HEX5563977.1"
                     /translation="MDNMFKLCGFWTGIFAVMFFVGGMMTASVIMVASTIFFLLLGYL
                     NLTERMYMYMFGAYLMIFMVGFSYYATFIHVPGAGH"
     gene            1562..2569
                     /gene="comGA"
                     /locus_tag="VFX34_03380"
     CDS             1562..2569
                     /gene="comGA"
                     /locus_tag="VFX34_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HEX5563978.1"
                     /translation="MENRENLIEKKCFELLERAIADEATDIHLVPMQDRYDISFKKNS
                     KLERSGTFPPQLAARMISFYKFLSSLDISDRRKPQSGSFHKKMHDENFSFRVSTIPSI
                     QMKESVVIRIQRHDQIVPLDMLCIEPVWEMKLRHAAEEGQGLVLLTGPTGSGKTTTIY
                     SLTAHCVNQLERHVISLEDPVENNHSHLLQIQVNERSGMTYAAGLKAILRHSPDVIMI
                     GEIRDSETAKTAVAAALTGHLVLSTVHSKDPEGCFYRMMDFGITPEELRQTVVCISAQ
                     RLIRKKDGEVGAVFEIVQGELLESIADGIISGEQVTVPKDMTIDAIMKKYSDEEMMPY
                     G"
     gene            2643..>3576
                     /gene="comGB"
                     /locus_tag="VFX34_03385"
     CDS             2643..>3576
                     /gene="comGB"
                     /locus_tag="VFX34_03385"
                     /inference="COORDINATES: protein motif:HMM:NF041012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HEX5563979.1"
                     /translation="MLEGYTFHDGLVLLMPYHSKRYSELLEQAERDLKNGLGVTEILR
                     TLGFSSNVLLPVIIAEVDGRLADALKGIAERMKQSEERKRKLRNLLLYPIMLFTFLAI
                     FLFAFRTYFLPNLQAMAVAKNGTATGFASMLPLIVSRIPDVMIGLGFFLAISAGIGIL
                     IYRKLPSPGKIRFLLSIPFAGSLFSKLKTRDFSGEIGSLLQSGQSMQDALDVLIEQEV
                     DPILREIAETIKSHVIYGENFDSAIHMTDGLRKELSAYAKHGSDTGHLGKELLLFSEN
                     LHGMIEEEMARWLSLLQPILFTILSIFILAAYLAL"
BASE COUNT         1055 a          728 c          830 g          963 t
ORIGIN      
        1 acaagtgcag gtggatatcc ttggaagacg attgttgcaa ttgtaaaacc gccaaaaatc
       61 acggcagctg caacattgaa caaaatgctc aacacattgc gctctttgaa agcttgtaca
      121 gtaccaatga cagcgaagat cgcaacgagt ccgaaaatta ccattaagat gttcatgtgg
      181 aagacactcc tttcaataga gaaaaaatga atgccgattt agcaatattc ctcttctatt
      241 gtactgtttc acacaaattt tgtcgaggca tattaatgac gattgtttga aatccagtta
      301 taagttcatt actatcaagt gtaggaggtg tgaataatgt tagaaatacg tacatatcca
      361 ttaggtccga ttcaaacgaa ttgttatgta atctacaatc gtgaagggga atgtttagtg
      421 atcgacccgg gagaagaagg ggatcgaatc attgcagaaa tcgaaaaaat gaatggcaaa
      481 ccgcttgcca tccttttgac acatgcgcat tttgatcata tcggagcggt ggatcgagtg
      541 aggaatcatt ttgatgtacc tgtccatatt cacgaagccg aaaaaaattg gcttggaaac
      601 ccggatctga acggttcctc aagatacccc ggtttgcctc atgtgaaaaa taaggcggcg
      661 gaccaattcc tgcaggaagg tgaattgcag attggttcat tccgattgga ggttcgtcat
      721 actccaggcc attctccagg tagcgtttct ttcattttca aggatgcaca aatggcggtt
      781 gttggggata cacttttccg gggcagcgtc ggcaggacgg atcttcccgg gggcgatacc
      841 aaaactttgc ttcaatccat ccatgagaag cttttgattt tggatgatga tttcatcgtc
      901 tatcccggcc atggcccggc aacaacccct gaagaggaaa aggatatgaa cccgtttttg
      961 aacggatttt aataaaaaga aaaaacagcc gagtggctgt ttttttcttt aggacgatta
     1021 atgtcctgca cctggtacgt gaatgaaagt cgcataatag ctgaatccta ccatgaaaat
     1081 cataaggtaa gctccaaaca tgtacatata catacgctct gtcagattca aatatccaag
     1141 caacaggaag aaaatagtgc tagcgaccat gataacggaa gctgtcatca tgccgccgac
     1201 gaaaaacatt acagcgaaaa tgccagtcca aaaaccgcat agcttgaaca tgttatccat
     1261 aagtgcgtcc ctccttaacg taaaacaata caaagatctc ttattcatta taatggatag
     1321 cagcttgaaa agtaaactcc aatcaagtcg acattgtgac aattgcatgg aacgcaaatt
     1381 gaatcaaatt atagtgaaat tgatttttga gcagaaaacg ccatgtgaaa tttatatttg
     1441 gggcggaaac ggcagtaaaa attcgatttt atcgggaagt attccctttt cgattgcttc
     1501 aacgtatact catcttaagg tgaaaagcgg tctttcatct ttattgaaag aggggtgttc
     1561 aatggaaaat cgtgaaaatc tcattgagaa aaagtgcttt gaacttttgg agcgggcgat
     1621 tgccgatgag gcgactgaca tccatctcgt tccgatgcaa gatcgttatg atattagttt
     1681 caaaaagaat tcaaaacttg aacggtccgg cacatttccc ccacagttgg ccgcccgtat
     1741 gatctccttc tataaatttc tctcctcatt agatatcagt gatagacgaa aaccccaaag
     1801 cggttcattt cacaagaaaa tgcatgacga aaacttttcg ttccgtgtat cgacgattcc
     1861 ctctatccaa atgaaagaaa gtgttgtcat ccgtattcaa aggcatgatc aaattgtccc
     1921 actggatatg ctttgcattg agcccgtgtg ggaaatgaag cttcggcatg ccgctgaaga
     1981 ggggcaagga cttgtcttgc tgaccggtcc cactggaagc gggaaaacaa caacaatcta
     2041 ctcccttacc gcacattgcg tcaatcagct tgaaagacac gtgatttcac tcgaagaccc
     2101 agttgaaaac aatcattcac atcttcttca gattcaggtg aatgaaagat caggaatgac
     2161 atatgctgcc ggattgaaag cgatattgag gcattccccg gacgttatca tgattggtga
     2221 aatccgggat tccgaaactg ctaaaactgc cgtggcggca gcgttgacgg gacatctcgt
     2281 cctgtctacc gtacactcga aagatcccga aggctgcttc tatcggatga tggatttcgg
     2341 gatcactccg gaggaactga ggcaaacggt cgtatgcatt tcggcgcaac gtcttattcg
     2401 gaaaaaagat ggcgaagtcg gggctgtgtt tgaaatcgtt caaggcgaac tgttggaatc
     2461 gattgcggat ggaatcatat caggtgaaca ggtgactgtg ccgaaggata tgacgattga
     2521 tgcgataatg aaaaaatata gcgatgaaga aatgatgccc tatggttaag gatttgttat
     2581 tcaggcgaac cacacaaagc attgataacc ggccagtatt tttagggagg ctttcgctcc
     2641 tcatgctaga aggctacaca ttccatgatg gattggtctt gcttatgccc taccattcga
     2701 agcggtatag tgaactgctg gagcaggcgg aaagggattt gaagaatggt cttggtgtga
     2761 cggaaatact tagaacgctt ggtttttcat caaacgtcct gttgccagtc atcatagcgg
     2821 aagtggatgg cagattggcg gatgcattga aagggattgc tgaaagaatg aagcaatcgg
     2881 aagaaagaaa gaggaagctg cgaaaccttc tgctctatcc aatcatgctg tttaccttcc
     2941 ttgctatctt tctattcgct ttcagaacct atttccttcc gaatctgcag gcgatggcgg
     3001 tagcaaagaa tggaacggca accggttttg cgtctatgtt gccactcatt gtttcaagga
     3061 ttcctgacgt gatgatagga ttgggttttt ttcttgccat atcggcaggc ataggaatcc
     3121 tgatctatcg taaattacca tcacccggaa agatccgctt ccttttgtcc attccgtttg
     3181 ccggttcatt attttcgaaa ttgaaaacaa gggacttctc aggtgaaatt ggaagccttc
     3241 ttcaatcagg ccaatctatg caggatgcac ttgacgtgct gatagagcaa gaagtagatc
     3301 cgattctacg tgaaattgcg gagacgatca aaagccatgt catctacggg gagaatttcg
     3361 attcggctat ccacatgaca gatggtcttc gaaaggaatt aagtgcgtac gcgaagcatg
     3421 gatccgatac agggcattta ggcaaggaac tgcttctgtt cagtgaaaat cttcatggca
     3481 tgatcgaaga agagatggca agatggctat cgttgctgca gccaatattg ttcacgattt
     3541 tatccatctt cattttagcg gcatatttgg ctttgc
//
LOCUS       DASULI010000156         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1754203,
            whole genome shotgun sequence.
ACCESSION   DASULI010000156 DASULI010000000
VERSION     DASULI010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754203"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFX34_03390"
     CDS             <1..636
                     /locus_tag="VFX34_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEX5563980.1"
                     /translation="HYKTLDIKDVDAVLLLHMCAFQSDGSQFPTPSDFAARMHLTENQ
                     VSEILQRFMQRGFLEIRQGQDENGVLYEQFSMQPLWNRLLNIVLTEQNVKEEQKDKDE
                     EGEIFALFEQEFGRLLSPMECETITMWLDEDGHAVKIIKAALKEAVLAQKLSLRYIDR
                     ILFEWKKKRVRTLNDVEKQSRSFRTSMMRPVQNQETVVKKVPFYNWLEERE"
     gene            647..1297
                     /gene="nth"
                     /locus_tag="VFX34_03395"
     CDS             647..1297
                     /gene="nth"
                     /locus_tag="VFX34_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5563981.1"
                     /translation="MLTKTQWEICLEEFGRMFPDAHCELVHENAFELLVATLLSAQCT
                     DVLVNRVTADLFQKYKTPEDYLAVDIEELQNDIRSIGLYRNKAKNIQALSALLIEEFD
                     GEVPPDRDTLTTFPGVGRKTANVVVSNAFGIPAMAVDTHVERVAKRLGMNRWKDTPLM
                     VEEKIMRWTPMEKWTDTHHQIIFFGRYHCKAQNPACGECPLLTLCREGKKRMKGIA"
     gene            1290..1634
                     /locus_tag="VFX34_03400"
     CDS             1290..1634
                     /locus_tag="VFX34_03400"
                     /inference="COORDINATES: protein motif:HMM:NF044560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563982.1"
                     /translation="MHNSDTYRKEMLYSFWNNWLDMKDRIEEAYKQKNSEAVNMLDKA
                     IENYLAMLQSFVYVGSSTLDRPTSMIEPLNGKERLQFIRTKRRGYFAFVQLDALYTEA
                     MKKAVSRLAMSK"
     gene            complement(1681..4344)
                     /locus_tag="VFX34_03405"
     CDS             complement(1681..4344)
                     /locus_tag="VFX34_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX5563983.1"
                     /translation="MSDQTKSRAARRRQIEAEKKRGKNKKKNNGGIIKKIILSLVAIG
                     VAVLVGGAGLFAFYASSAPELDESLLKDPLTSDIVDRNGNVFMKFGAEKREFVPYDQI
                     PKEMENAILATEDIRFYKHHGMDFWRLGGAVLANFRSGFGSQGASTLTQQVIKNSFLS
                     DEKTLKRKAQEAWLAFKLEREYSKEQIFEMYFNKILMSGNIYGIGTAAKEFYGKDLNE
                     LELHEMALLAGMPQSPNGYNPFKNPERAEKRRNTVLSLMYKHGKITKEQMEKAKAIPV
                     TETLLPEDQLQQNKDKKYLAYVDIVLDELEAAGMMDVLAEGVTIQTALDPAAQQSVEK
                     AINSPIYESDEMQAGMTVLNTKTGEIVAIGGGRNFTGRNLNFASGDNRRMPGSVIKPI
                     LSYGPIIEYENWSTAHIVVDEPYKYKGTNISIRNVDGKFMGPISAREALYRSRNIPAI
                     KVFEEVGTKRAREFADKLGLKYGDMTSTLAIGGGDGEFSTIQMAGAYAPFGNGGIYTK
                     PHAVKKIIFRDGKTERNLSPDPVTVMKDSTAYMVTDMLRDVISFGTGKTAGVSGLDMA
                     GKTGTTNYDAAKMKELGLKSTDVPDSWFTGYTTEYTISVWGGYKNRNTPITTYDRGRY
                     VPQILFRNVMTDLVSGKKTPRFKQPSSVEEAVIVKGSNPPILASNSTPDALKSTELFV
                     RGTVPTEMDEEYITELEAPSGLSAQYDAETNSVSLNWSYDNPDAYMLPEPVQFNISVS
                     VDGSEPQNMKTISENNITFSGVEMGRTYVFYVKAISGEIESSTVSTSLLIEGQTEDDP
                     GMDDGDESGNNGGNNGQNNSGNNGNNNGNNGNSGNNGNDGNDGSNNDGNDEGTDNPPD
                     DGSGSSGDETTDPGTGGSDGSGDGTGSGSGR"
BASE COUNT         1127 a         1039 c          995 g         1303 t
ORIGIN      
        1 cactataaaa cattggatat aaaagatgtc gacgcggtgc tacttttgca tatgtgtgca
       61 tttcagtcag atggttcgca atttccaaca ccatccgatt ttgctgcacg catgcatctt
      121 acggagaatc aagtatccga aatccttcag cgcttcatgc aacgcggatt cttggaaatc
      181 cggcagggac aggatgaaaa tggagtgctc tatgaacaat tttcgatgca gccattatgg
      241 aacaggctat tgaacattgt gcttacagaa cagaacgtaa aagaagagca aaaggataag
      301 gatgaagaag gcgaaatctt tgcgttgttc gagcaggagt tcggccgtct cttatctccg
      361 atggaatgcg aaacgatcac gatgtggttg gatgaagatg gacatgctgt caagatcata
      421 aaagcggcat tgaaagaggc tgtccttgca caaaaactca gtttgcgcta tattgaccgg
      481 atcctctttg aatggaagaa aaaaagggtc agaacattga atgatgtgga aaagcaatcc
      541 aggtcattca ggacatcaat gatgcgtccg gttcaaaatc aggaaaccgt agtaaaaaag
      601 gttccttttt ataattggct ggaagaacgg gaataggtga ttgcatatgt tgacgaagac
      661 acaatgggaa atctgtctcg aagaattcgg aaggatgttt cccgatgctc attgcgagct
      721 ggtgcacgaa aatgcttttg agctgctcgt tgccacattg ctctccgctc aatgtacgga
      781 tgtactcgtg aatcgtgtta ccgcggattt gttccaaaaa tataaaacgc ctgaagatta
      841 tttggcggtt gatattgaag agcttcaaaa cgatattcgg tcgatcggcc tgtatcggaa
      901 caaagcgaaa aatattcaag cattgagcgc gcttttaatc gaagagtttg atggggaggt
      961 cccacccgat agggatacgc ttacgacttt tccgggagtc ggaaggaaga cggccaatgt
     1021 tgtcgtttcc aatgcgttcg gcatccctgc aatggcggtc gacactcatg ttgaacgagt
     1081 tgctaaacgc ctcggcatga atcggtggaa agacacgcca ctcatggtgg aggaaaagat
     1141 catgcgttgg acgcctatgg aaaaatggac agatacgcat catcagatca ttttcttcgg
     1201 gcgatatcat tgcaaagccc aaaatcctgc ctgcggggaa tgcccgctcc tgacgttgtg
     1261 ccgggaagga aagaaacgga tgaagggcat tgcataattc cgatacctac aggaaggaaa
     1321 tgctttattc tttctggaac aattggctgg acatgaaaga cagaatcgag gaagcctata
     1381 aacaaaagaa ttctgaagcc gtaaatatgt tggataaggc aattgaaaat tatttggcta
     1441 tgcttcagtc atttgtgtat gtcggctctt ccactcttga ccgtccaaca tcaatgattg
     1501 aaccgttgaa tgggaaagag cgactgcaat tcatccgcac taaaagaaga gggtattttg
     1561 cattcgtcca attggatgca ttgtacaccg aggcgatgaa aaaagcggtt agccgtttgg
     1621 caatgagtaa ataaaacagc ctgaagtgat gtaaatcatc acttcaggct gttttttaag
     1681 ttatcttcca ctaccactgc cggttccgtc gccggatccg tcactacctc ccgttcccgg
     1741 gtcagttgtc tcgtctcccg atgagccgga tccatcatcg ggcggattgt cagtgccttc
     1801 gtcatttcca tcattgttac tgccgtcatt cccgtcattt ccattgttgc cgctattgcc
     1861 gttgtttccg ttgttatttc cgttattgcc gctattattt tggccgttgt ttcctccgtt
     1921 atttccactt tcatccccat catccatccc agggtcatct tccgtttgtc cttcaattag
     1981 aagggaagtg gaaactgtac tactttcaat ttcccctgaa atcgccttga cgtagaagac
     2041 gtatgtgcgg cccatttcaa ctcctgagaa cgttatgtta ttttccgaaa ttgttttcat
     2101 gttttgaggc tcactgccat ccactgaaac ggagatattg aattgtacag gttccggaag
     2161 catataggca tcaggattgt cataggacca atttaatgaa acagagttcg tctctgcatc
     2221 atattgagca gacaagccgc ttggcgcttc cagttcagta atatactctt catccatttc
     2281 ggtcggaaca gttcctctta caaaaagttc agtgctcttt aacgcatcag gcgtcgaatt
     2341 gctcgcaagg attggcggat tggacccttt tacgataacc gcctcttcaa cagagctcgg
     2401 ctgcttgaac cgtggcgttt tcttaccgga cacaaggtcg gtcatcacgt ttctgaacag
     2461 gatttgtggc acatagcggc cacgatcata cgttgttatc ggtgtattcc ggtttttata
     2521 tccaccccaa acggaaatag tgtattccgt cgtataaccg gtgaaccagc tatccggtac
     2581 gtctgtactt ttcagcccga gttccttcat cttagccgca tcatagtttg tcgtacccgt
     2641 cttaccggcc atatccaatc cggatacgcc tgctgtcttc ccggttccaa aagaaattac
     2701 atcccgtaac atatcagtaa ccatataggc agtagaatct ttcatgaccg tgacaggatc
     2761 cggtgacaag ttacgttcgg tttttccatc acggaaaatg attttcttaa ctgcgtgagg
     2821 ttttgtataa ataccgccat tgccgaacgg agcataggct cctgccattt gaattgtaga
     2881 aaattcacca tctcctccac cgattgcaag agtggaagtc atatccccat atttcagccc
     2941 aagtttatct gcaaattcac gggctctttt tgtcccgact tcctcaaaca ctttaatcgc
     3001 cggaatgttt cttgaacgat acaatgcttc cctcgctgaa atcggtccca tgaatttccc
     3061 atccacgttc cggatagata tgttagtgcc tttgtattta tatggttcgt caacaacgat
     3121 atgggcagtt gaccagttct cgtattcaat gattggacca taggaaagga tcggtttgat
     3181 gaccgagcct ggcattctgc ggttgtcgcc tgaagcgaag ttcagattcc gcccggtaaa
     3241 attccttcct ccgccgattg cgacgatttc gccggttttt gtattaagga ccgtcattcc
     3301 agcctgcatc tcgtcagact cataaatcgg agagttgatc gccttttcga ccgattgctg
     3361 tgccgcagga tcaagtgcgg tctggatcgt tacgccttct gcaaggacat ccatcatgcc
     3421 ggccgcttcc aattcatcca atacgatgtc aacataggca agatactttt tgtccttgtt
     3481 ttgttgaagc tgatcttcgg gaaggagtgt ttccgtgact gggattgctt tcgccttctc
     3541 catctgctcc ttcgttattt ttccgtgttt gtacataagg ctaaggacag tattccggcg
     3601 cttttcggcc ctttcaggat tcttgaacgg attatagccg ttcggggatt gaggcatccc
     3661 tgccaacagc gccatttcat gcaattcaag ttcattcagg tctttgccat agaattcttt
     3721 agcagctgta ccaattccgt atatattgcc tgacataagg attttattga aatacatttc
     3781 gaaaatctgt tcttttgaat actctcgctc tagcttgaag gcgagccacg cttcctgcgc
     3841 tttccgcttc aatgtctttt catccgatag gaaagagttc ttgatgactt gctgcgtgag
     3901 cgtagatgca ccttgcgatc cgaaaccgct tctgaagttt gcaaggaccg ctccgcccag
     3961 tctccagaaa tccattccat gatgcttata gaagcggatg tcttcagttg ctaggatggc
     4021 attttccatc tctttcggaa tttgatcata tggaacgaat tcccgttttt cggctccgaa
     4081 tttcatgaac acatttccat tgcggtcgac aatatcggac gtgagcggat ccttcaataa
     4141 actttcatca agttctggtg cactgctcgc gtagaatgcg aatagacccg cccctccaac
     4201 caatactgca acaccgatcg ccactaggct gagaatgatt ttcttaatga tgccgccatt
     4261 attcttcttt ttattttttc cgcgcttttt ttcagcttct atctgccgac gtctggcagc
     4321 gcgagatttt gtctgatcac tcataagttt tcctgccttt caatcgtttc taattcaggt
     4381 ttctgcaaaa gtgaatccaa cgcggccaaa taatcaagcc gaggattgaa acctgcattt
     4441 atttctatgg atatctcttc catt
//
LOCUS       DASULI010000157         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_708867, whole
            genome shotgun sequence.
ACCESSION   DASULI010000157 DASULI010000000
VERSION     DASULI010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708867"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VFX34_03410"
     CDS             complement(<1..280)
                     /locus_tag="VFX34_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5563984.1"
                     /translation="MKKSFVLTVLTVLVVGILSACGSSASGNKSVKIGYFPNLTHIAT
                     IVALENGYFEEEFGDDVKIETKTVSNGGLFMEAMTTKSIDVGTVGPGPL"
     gene            complement(304..1056)
                     /locus_tag="VFX34_03415"
     CDS             complement(304..1056)
                     /locus_tag="VFX34_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5563985.1"
                     /translation="MTTALRRIIFIAMIAVIWEVTSRLSVLPEFMFPRLTQVLETLFN
                     GLVNGQFLAAIGKSMSRLLIGFAVAIVLGVIMGYLIWRYKLVEDTLGFLVTALQSIPS
                     IVWFPLAIIWFGLNDFSILFIVTIGATWTMTVSATSGFRNVPTLYQRVAKTFGSTGVH
                     FIRTVILPASVPQLLSGLRIAWAFSWRALMAGELLGSGGGLGQLLETGRSLGQMDLVI
                     SVMIIIAVIGTIMDNVVFLRLERSVQKKWGLA"
     gene            complement(989..1831)
                     /locus_tag="VFX34_03420"
     CDS             complement(989..1831)
                     /locus_tag="VFX34_03420"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5563986.1"
                     /translation="MYVTIDGIEKSFINENNEPVHVLDHIDIDMEKGSFVSIVGPSGC
                     GKSTLLYMIAGLEKADKGSIRIAGNEVKKPGPDRVVVFQEAGLFPWLTVLENVKYGLL
                     LKKMPKQEAEAKAIDMLKMVHLSNYIHSYPHQLSGGMKQRVSIARALVMEPDVLLMDE
                     PFSALDEQTRMVLHKELIDIWKKTKVTIFFVTHNIREAVLLSEKVIVFGTRPGKVKEI
                     IPVPTTTDGVLPDSVTLHTEQRILAILEEEIEKVLKEEMGDDYSFKTNHLHRNDSGDM
                     GSHI"
     gene            complement(1897..>2275)
                     /locus_tag="VFX34_03425"
     CDS             complement(1897..>2275)
                     /locus_tag="VFX34_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5563987.1"
                     /translation="TGEYLKDYDSGITVHVAEPAGSPVLSGGKPGKHKLVGTSPGFIP
                     SILNTSVYDEIFKIEDEDAYQTVRELAAREGLLLGPSGGASVYAAMQVAKRLTPEDTV
                     VCIAPDNGERYLSSDLFDNQGEE"
BASE COUNT          561 a          642 c          488 g          584 t
ORIGIN      
        1 gcaatgggcc agggccgact gtcccgacat cgattgattt cgtcgtcatc gcttccatga
       61 aaagaccgcc gttgctcact gtcttcgttt caattttaac atcgtccccg aattcctctt
      121 cgaaataacc gttttcaagg gcgacgatcg tggcgatatg cgtcaagttc gggaagtacc
      181 cgattttcac cgatttgttg cctgacgccg aagaaccgca agcagataag attcccacta
      241 ccaaaaccgt taacactgtc agtacgaatg actttttcat tgtggatttc ccctttgtaa
      301 aggtcatgcc agaccccatt ttttctgtac agaacgctcc aaacgtaaaa acacgacatt
      361 atccattatc gtcccgatca ccgcgatgat gatcatgacc gatatgacga ggtccatttg
      421 accgagcgat cgccctgttt cgagcaattg ccccagccct ccgcccgagc cgagcaattc
      481 ccctgccatc aatgcccgcc aggaaaaagc ccatgcaatg cggagtcccg ataataattg
      541 cggaacggat gccggcaaga tgacggttcg aatgaaatga acccctgtcg agccgaatgt
      601 tttggcgacc cgttggtaca acgtcggcac attccggaat ccactcgtcg cactgacggt
      661 catcgtccat gtcgcgccga ttgtgacgat gaacagaatc gagaagtcat tcaacccgaa
      721 ccagatgatg gcgagcggga accatacgat gctcggaatg gattgcaacg cggtgacgag
      781 aaagccgagt gtatcctcga ccagcttata gcgccagatg agatagccca tgatgacacc
      841 taaaacgatc gctaccgcaa acccgatcag caggcggctc atgcttttcc cgatcgccgc
      901 taaaaactgc ccgttcacga gaccgttgaa aagcgtctcc aatacttgtg tcaaacgtgg
      961 aaacatgaat tccggcaata ccgaaagtct agatgtgact tcccatatca ccgctatcat
     1021 tgcgatgaag atgattcgtc ttaaagctgt agtcatcacc catttcctcc ttcaacacct
     1081 tttcgatttc ctcttccaaa atggctaaga tccgctgctc cgtatgcaaa gtcacactgt
     1141 ccggcaatac gccgtccgtc gttgttggaa ctgggatgat ctccttcact tttccggggc
     1201 gcgtgccgaa gacgatcact ttttccgata ataaaacagc ttcccggata ttatgcgtaa
     1261 cgaaaaagat cgtcactttc gtcttcttcc atatatcaat caattcctta tggagcacca
     1321 tcctcgtctg ttcatcgagt gccgaaaacg gttcatccat caataaaaca tccggctcca
     1381 tcactaacgc tcttgcaatc gaaacccttt gcttcatccc gcccgagagt tggtgcggat
     1441 acgaatggat atagttgctc aggtgcacca ttttcaacat gtcaatcgct tttgcttcgg
     1501 cttcctgttt cggcatcttc tttaacagca atccatactt cacgttttcc aagacggtca
     1561 accacgggaa gagaccagcc tcctggaaaa cgacgacccg atcgggacca ggcttcttca
     1621 cctcattccc cgcaatccgg atactgcctt tatccgcctt ttccaatcct gcaatcatat
     1681 agaggagcgt cgatttgccg catccggaag gaccgacgat ggagacgaag ctcccttttt
     1741 ccatgtcaat atcaatgtgg tccagcacgt ggaccggctc gttgttttca ttgatgaaac
     1801 tcttttcaat cccatcgatt gttacgtaca tcttcaacac ctcgacttat cttattcgta
     1861 tagtcggaat taatgttatg aaaacggaga ggacttttat tcctctcctt ggttatcaaa
     1921 taaatcactg gatagatagc gttctccgtt atcgggtgca atgcagacga ccgtatcttc
     1981 cggcgtcaat cgtttcgcta cttgcatcgc cgcatagacg gaagcccctc cgctcggacc
     2041 gagcagcaga ccttcacgag cagccaattc ccggactgtt tgataggcgt cctcgtcttc
     2101 gatcttgaaa atctcatcat acaccgatgt gttcaatatt gacggaatga aaccggggct
     2161 cgttccgaca agtttgtgtt tccctggctt cccgccggac agtaccgggg atccggcagg
     2221 ttccgctaca tggactgtaa tcccgctatc gtaatccttc agatattcac cggtt
//
LOCUS       DASULI010000158         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_331743, whole
            genome shotgun sequence.
ACCESSION   DASULI010000158 DASULI010000000
VERSION     DASULI010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331743"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFX34_03430"
     CDS             complement(<1..496)
                     /locus_tag="VFX34_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5563988.1"
                     /translation="MIQRIVMVGSGVMGRGIAYVGAVGGYSVTLVDINEMALQHAKDE
                     IEAIFEKGMKYGKISADTAENARQKLTYETKLAEAAGRADLIIEAVPELASIKQQVFE
                     ELEQYASKECYFATNTSTMSPTEIASFGKRPDKTIAMHFFNPVHKMPLVEIVRGLETS
                     DGTVE"
     gene            complement(618..2135)
                     /locus_tag="VFX34_03435"
     CDS             complement(618..2135)
                     /locus_tag="VFX34_03435"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5563989.1"
                     /translation="MTTIQEGTVERESMKRDYYKLFINGEQVDSSSGERTKIYNPATG
                     ELLAEVAKATKEDAEKAVRAARDSFDNGKWKITPAGRRARVLNKIAAIMGERFNELVE
                     LEILNTGKSLAAAKGQIAQAIEDFEFYAGAIVGHGGSVNNVPGQFHNYTEKEPLGVCA
                     QIIPWNYPLMMAAWKIAPAIAAGCSVVIKPASLTPLTAIVLGEICHEAGVPAGVVNIL
                     PGSGSEIGNYLVEHELVNKVAFTGSTSIGKDIMAKAAGTVKRVTLELGGKSPSLVFED
                     ADIDAAVDGSLYGAFNNTGQSCDARSRIYIHENVYDEFVEKFIAKTEKLAIGDPFSKA
                     THMGAVIDQTQLDTVKMYVQSAIDEGAEILTGGKELKPEGFENGFWYAPTIIGNVTQD
                     MKVVREEIFGPVVVVSKFKDEKEAIALANDSDYGLASSVWSTNTATTTRVANQIQAGI
                     VMINTPFSAFPGTPFGGFKQSGFGRELSAGSLDLYTETKSIMSYFGSRPVNPFGL"
     gene            complement(2149..>2542)
                     /locus_tag="VFX34_03440"
     CDS             complement(2149..>2542)
                     /locus_tag="VFX34_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5563990.1"
                     /translation="VNAFIHVGLIPDSGNLYYLTQLVGHAKATELAMLGEKVPAADAV
                     AMGLANRLILADTWEEDVAAFAARLAALPTKAIGLIKRSLKAAPALSFEDYLEQEAQG
                     QRIAGLTKDHREGVNAFMEKRKPVYSGQ"
BASE COUNT          596 a          661 c          535 g          750 t
ORIGIN      
        1 tttccaccgt cccatcactt gtttccaatc cacggacaat ctctacgagc ggcattttat
       61 gtaccggatt gaaaaagtgc attgcaattg ttttatccgg acgtttaccg aaagaagcga
      121 tttctgtagg actcatcgta gatgtatttg ttgcaaaata acactctttc gaagcatatt
      181 gttccagttc ttcaaacact tgttgtttaa tggatgccag ttcaggtacc gcttcgataa
      241 tcaaatcagc gcgtcctgcc gcttccgcta gcttcgtttc atacgtcagt ttttggcgcg
      301 cattctcggc tgtgtctgct gatatttttc catatttcat tcctttttca aaaatagctt
      361 cgatttcatc ctttgcatgt tgaagtgcca tttcgttaat atcgacaagt gtaaccgagt
      421 acccgccaac ggcaccgaca taggcgattc ctctgcccat gacaccggag ccgaccataa
      481 cgatacgctg aatcatccgt attcctcctt ttcacgatga aagaatacgg acaggagatc
      541 aaccattcct gtccgtcatt tttactaata ggtatacgtt tttataaccg agaaaccact
      601 tgcgccttcc gcttttctta tagcccgaat ggatttaccg ggcggctgcc gaagtacgac
      661 atgatgcttt tcgtttctgt gtaaagatca agcgatccgg ctgacagctc acggccgaat
      721 cctgattgtt taaagccgcc gaatggtgta cccgggaatg cagagaacgg cgtgttgatc
      781 attacgatgc ccgcttgaat ctggttcgca acacgtgttg tcgttgcagt atttgtcgac
      841 caaacagatg aggctaaccc ataatccgaa tcatttgcca aagcaattgc ctctttttcg
      901 tctttaaact tcgatacgac gacaaccgga ccaaagattt cttcacggac aactttcatg
      961 tcctgcgtta cgtttccgat aattgtcggc gcataccaaa acccgttttc aaagccttcc
     1021 ggtttcagtt ctttgccgcc cgtcaggatt tcagcacctt catcgattgc agattggaca
     1081 tacattttca ctgtatccaa ctgggtttga tcgattacgg cacccatatg tgttgccttg
     1141 ctaaacggat ctccgattgc gagtttttca gttttcgcaa tgaatttctc aacgaactca
     1201 tcgtacacat tttcatgaat atagatgcgt gaacgcgcat cacaagattg acccgtgtta
     1261 ttgaatgcac cgtatagtga accatcgacg gctgcatcga tatcggcatc ctcgaaaacg
     1321 aggcttggag atttcccgcc aagttccaat gttacgcgtt ttactgttcc tgccgctttc
     1381 gccataatgt cttttccgat tgacgtggaa ccggtgaacg caactttatt cacaagttca
     1441 tgttcaacca agtaattgcc gatctcggaa ccagatcccg gcagaatgtt aacaacgcct
     1501 gctggaacgc ctgcttcatg acaaatttct ccgagaacga tcgctgtaag cggcgtcagt
     1561 gacgcgggct taataacgac cgagcatcct gcagcaatgg caggggcaat cttccaagca
     1621 gccatcatca gcggatagtt ccagggaata atttgtgcac acacgccaag cggttctttt
     1681 tctgtgtagt tatggaattg gccaggtacg ttgtttacgg atccaccgtg tccaacaata
     1741 gcgcccgcgt aaaattcaaa gtcttcgatt gcttgtgcaa tctgcccctt cgcagctgca
     1801 agtgattttc ctgtattcaa gatttccagt tccacaagtt cgttgaagcg ttcgcccatg
     1861 attgccgcaa ttttgttcaa tactcgcgca cggcgtcccg caggagttat tttccacttc
     1921 ccattatcaa atgaatctct cgcagcccgg acagcttttt cagcgtcttc tttcgtcgct
     1981 tttgccactt cggcaagcag ttcacccgtc gcaggattat agatcttcgt gcgctcccca
     2041 ctgctgctat cgacttgttc accatttatg aaaagcttgt aatagtcacg cttcatcgat
     2101 tcccgctcaa ctgttccttc ttgaattgtc gtcattggat gattcctcct attggcctga
     2161 atagacaggt tttctttttt ccatgaatgc gttgacacct tcgcgatgat cttttgtaag
     2221 acccgcaatc cgctgtcctt gggcttcttg ttcaagataa tcctcaaaag atagagcggg
     2281 agccgctttc aatgaacgtt tgatcaaccc gattgctttt gttggcaatg ctgcaagccg
     2341 ggctgcgaat gcggcaacat cttcttccca tgtatctgcc agaatcagcc ggttcgcaag
     2401 gcccattgcc acggcatcag ccgccggcac tttttctccc aacatcgcta gttccgttgc
     2461 tttggcatgt ccgacgagtt gtgtcagata atagagattg ccggaatccg gaatcagacc
     2521 aacatggatg aaggcgttta ca
//
LOCUS       DASULI010000159         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1071295,
            whole genome shotgun sequence.
ACCESSION   DASULI010000159 DASULI010000000
VERSION     DASULI010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071295"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VFX34_03445"
     CDS             complement(<1..1327)
                     /locus_tag="VFX34_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5563991.1"
                     /translation="MTTETKEAMQSPSHWTAHLELVAAIISGVLILAAWLIGKEGSPA
                     LSVSLYMIAFLIGGFAKAKEGIEETIADRQLNVEMLMVFAAIGSAVIGYWAEGAILIF
                     IFAISGALETYTLNKSHKEISALMELQPEEAWLIQEDGSTVNVATSSLSIGSKILVKP
                     GERIPVDGVVIQGTTSVDMSAINGESVPVTKAENDELFAGTVNLSGAIHMEMTKPSSE
                     TLFQKIITLVQNAQSEKSPSQQFIERFEGTYVKIVIFTVIVMMFLPHYLFGWDWTTTF
                     YRAIVLLVVASPCALVASIMPATLAAVSNGAKRGILFKGGVHLEHLGSLKAIAFDKTG
                     TLTSGKPVVTDFIVREHADAHKALAMLSGIEAQSNHPLAVAIVNYAEEQGVFQRENPQ
                     IEDVPGFGIRAWTESGEVLVGNPRFVGKELIDRFNGGIVETLSNEGKTVV"
     gene            complement(1561..>2202)
                     /locus_tag="VFX34_03450"
     CDS             complement(1561..>2202)
                     /locus_tag="VFX34_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5563992.1"
                     /translation="NEGEKLSFEQEEQAEVKQSSRVVELTAPISEEQIRDLKVGDVVK
                     ISGRMYTGRDAIHKHLSENDAPVDLDGHIIYHCGPVVLKNDGGGYEIVAAGPTTSIRE
                     EPYQGDIMKKFGIRAVIGKGGMGPKTLAALQEHGGVYLNAIGGAAQYYADCIKAVEGV
                     DLLQFGIPEAMWHLRVEDFTAVVTMDSHGNSLHADVDQSSLEKLSQFKEKVFS"
BASE COUNT          573 a          545 c          511 g          573 t
ORIGIN      
        1 agacgacagt cttcccttcg tttgaaaggg tttcgacgat acctccattg aatcggtcga
       61 tcaattcctt accgacaaac ctaggattgc cgacgaggac ttcaccggac tctgtccaag
      121 cgcggattcc gaatccggga acatcctcga tttgcgggtt ctccctttgg aacacccctt
      181 gctcttcggc ataattgacg atggcaactg caagcggatg attcgattgt gcctcaatgc
      241 ctgaaagcat ggcgagagct ttgtgggcat ccgcatgttc acggacgatg aaatcggtga
      301 cgaccggctt cccggaagtc aacgtgccgg tcttatcgaa ggcgatggct ttcaatgaac
      361 caagatgctc cagatgaacg ccgcctttaa ataggatgcc gcgcttggcc ccgtttgaaa
      421 cagccgccag cgttgcgggc atgatcgatg cgacgagcgc acaaggggag gcgacaacca
      481 acaggacgat agcacgataa aatgtcgttg tccaatccca accgaagagg taatggggta
      541 aaaacatcat gacgatgaca gtgaaaatga caattttcac gtaggtgcct tcaaatcttt
      601 cgatgaattg ctgggacggc gatttttcac tttgcgcgtt ttggacaagc gtgatgattt
      661 tctggaataa agtttcagaa cttggcttcg tcatttccat atgaatcgcg ccgcttaaat
      721 tgacggtgcc cgcaaataat tcatcgtttt cggcctttgt gacaggaacc gattcaccat
      781 tgatggcgga catgtcgacc gaagtcgtcc cttggatgac aacgccatcg accggaatcc
      841 tctcacccgg tttcacaaga atctttgatc ctatggaaag ggaagaggtg gctacattca
      901 ccgttgaacc atcttcctgg atgagccatg cctcctcagg ctgcagttcc atgagggctg
      961 aaatttcctt atggctcttg tttaatgtgt acgtctcaag ggctccgcta attgcaaaaa
     1021 tgaaaatcag gatggcgcct tcagcccaat agccgataac agcggaaccg atggccgcaa
     1081 acaccatcaa catttcgaca ttcaactgtc tatcggcgat cgtttcctca atgccttctt
     1141 tcgcttttgc gaagccgcca attaaaaagg cgatcatata caaggagact gaaagggcag
     1201 gagacccttc ctttccgatg agccaagccg ccaaaatcag aaccccggaa atgattgcag
     1261 cgacaagctc taaatgtgct gtccaatgag aggggctttg cattgcttcc ttcgtttcgg
     1321 ttgtcattta catacctcct attgagaatt aatctcatct tcaataaatc gctttttatt
     1381 tgaaaacccc atattggtga agaaaaaagg gctgctttga ataaaataat aattgttatg
     1441 atgtgataat tattataaat aatagtatag caaattcaac tagaaagcaa cacccatgac
     1501 tcgacacgaa ggaaaggaca agaaaagccc cgacacgagt tgtgccgggg ctacagaata
     1561 ttaactgaaa accttttctt tgaattggga aagtttttca agggatgatt gatctacatc
     1621 tgcatgcagg ctgtttccgt gtgagtccat cgtgacgaca gccgtgaaat cctcgacacg
     1681 caaatgccac atcgcttcag ggatgccgaa ttggagaagg tctacaccct caaccgcttt
     1741 aatgcagtcc gcataatatt gtgcagctcc gccgattgcg ttcaagtaga caccaccatg
     1801 ctcctgcagc gcggcaagtg ttttcggacc cattccgcct tttccgatga cagcacggat
     1861 gccgaatttc ttcataatat caccttggta gggctcttca cgaatcgagg ttgtcggtcc
     1921 tgcagctacg atttcgtaac cgccgccatc atttttcaat acaaccggac cgcaatggta
     1981 gatgatgtgg ccatccaaat cgacaggtgc atcattttca gaaagatgtt tatggattgc
     2041 atcgcgccct gtatacatgc ggccgctgat tttaacgacg tcgccgactt tgagatcacg
     2101 gatctgctct tcgctgatcg gggcagtcag ttcaacgacc cgtgatgatt gtttcacttc
     2161 tgcttgttct tcttgttcga aggaaagctt ttccccttca tt
//
LOCUS       DASULI010000160         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1292724,
            whole genome shotgun sequence.
ACCESSION   DASULI010000160 DASULI010000000
VERSION     DASULI010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292724"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..277)
                     /gene="atpE"
                     /locus_tag="VFX34_03455"
     CDS             complement(65..277)
                     /gene="atpE"
                     /locus_tag="VFX34_03455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5563993.1"
                     /translation="MGLLAAALAVGLGALGAGIGNGLIVSKTVEGIARQPEARGMLQT
                     TMFIGVALVEALPIIATVIAFIVMNK"
     gene            complement(331..1044)
                     /gene="atpB"
                     /locus_tag="VFX34_03460"
     CDS             complement(331..1044)
                     /gene="atpB"
                     /locus_tag="VFX34_03460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5563994.1"
                     /translation="MNHENPEYLHFLGLNFNPANILMLFVTCLVVFIIAVLATRNLQI
                     KPTGMQNFMEWVMDFVKGIIKNNMDWKTGGRFHVLGITLIMFVFVANMLGLPAAVVWK
                     GELWWKSPTADPVITLTLAATVVILTHYYGVKQLGTKGYFKTYLQPMPFLAPLKIIEE
                     FANTLTLGLRLYGNIFAGEILIGLLATLGAGSAFGLVGALIPSLAWLGFSVFIGAIQA
                     FIFVMLTMVYMAHKVQTDH"
     gene            complement(1074..1448)
                     /locus_tag="VFX34_03465"
     CDS             complement(1074..1448)
                     /locus_tag="VFX34_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX5563995.1"
                     /translation="MHTLRQIFNRQKKALFFLLALFVIGWAFSDFRPFFAGMILGSLF
                     GLYNFFILVRRMDRFDQSVSKGKETKSIGGTALRFGSGVAAAAIALSMPEKFDLIGTV
                     VGLMIPYVLLLVDRIVVHVKHF"
     gene            complement(1977..>2401)
                     /locus_tag="VFX34_03470"
     CDS             complement(1977..>2401)
                     /locus_tag="VFX34_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX5563996.1"
                     /translation="DTQVIYPVHMNPAVREVADEILGGNNRVHLIEPLEVVDFHNFAA
                     RSHIILTDSGGIQEEAPSLGKPVIVLRDTTERPEGIEAGTLKLAGTDEETIFNLTDTL
                     LSDETEYSAMAKASNPYGDGHASERIVSALKEYLSTSD"
BASE COUNT          616 a          565 c          574 g          646 t
ORIGIN      
        1 tacataaatg aatggcgaag ggcatctgcc tcggtcttcg ccattttgta aagttgtttt
       61 cttattattt gttcattacg atgaacgcga taactgtcgc gatgatcgga agggcctcaa
      121 ctaacgctac cccgatgaac attgttgttt gaagcatacc gcgcgcttct ggttgacgag
      181 cgatgccttc tactgtttta gaaacgatca aaccgttacc gatacctgct ccaagtgcac
      241 ctaaaccgac tgcaagtgct gctgctaaaa gacccatagt gtattttcct cctaatagtt
      301 aaataaattt tattgttggt atatatatgc ttaatgatcc gtttggactt tatgcgccat
      361 gtagaccatc gttaacataa cgaaaatgaa tgcttggatc gctccgatga agactgagaa
      421 gcctaaccag gcgagtgatg ggatgagtgc tccgacaagt ccgaatgcac tgcctgcccc
      481 gagtgtcgca agaagtccga tgaggatctc ccctgcgaaa atattaccgt atagacgtag
      541 accaagtgtc aacgtgtttg caaattcttc gatgatctta agcggtgcca ggaatggcat
      601 cggttgaagg tacgttttga agtacccctt cgttccgagt tgtttaacgc catagtaatg
      661 ggttaagata acaaccgtcg cagcgagcgt caatgtaatg acgggatccg ctgtcggcga
      721 cttccaccaa agctcacctt tccagactac cgcagccggc aatccgagca tatttgcaac
      781 gaagacgaac atgatcaggg tgattccgag tacgtggaat cgtccacctg ttttccagtc
      841 catattgttc ttgatgatcc ctttgacgaa atccatgacc cattccatga agttctgcat
      901 gccggtcggt ttgatttgca gattgcgcgt cgctagaacg gcaatgatga atacgactag
      961 acatgttaca aagagcatga ggatgtttgc cgggttaaag tttaatccga gaaaatgaag
     1021 atactccgga ttttcatggt tcacgtttcg ttcacctccc ttatgcgtgt cagttagaaa
     1081 tgctttacgt ggactacgat tctgtcgacg agcaatagaa cgtatggaat catcagtccg
     1141 accactgtac cgatcagatc gaatttctcc ggcatcgaca acgcaatggc tgccgccgcc
     1201 acaccggatc cgaatcgcaa tgcggttcct ccgattgact ttgtttcttt ccctttcgaa
     1261 acgctctgat caaatctatc cattctacgg acaagaataa agaaattata cagtccgaac
     1321 aaggagccta agatcatccc tgcgaaaaac ggtctgaagt cactaaaagc ccatccgatg
     1381 acaaacaatg cgagcaaaaa aaagagtgcc ttcttctgcc tattaaaaat ctgtcgcagt
     1441 gtatgcatga tgggtgattc tcctgattcg tattttccgg gtccggcgat ttcgaaaagc
     1501 ggggtgtcgt acgaacggta taatccgttt ccctagtagg gcttgtactg atagagactc
     1561 cgaaaaaggt cggaacctat gtaagtgaat cgccgtaagc aactgaccac caggaggcgt
     1621 tgccatcgag cacgctcatg gggcatacgt ggaacgacgt ccagtgaagg atgatagaat
     1681 cgcccggaag cttgtcatcg tggcgtttat catatgtatg aatccctatc attcttaccc
     1741 tttgttagca tacaataggg gcattttgat gtcaattgat tcccgtgaaa ttcttcacaa
     1801 agtcttcacg ttgtcaaata atagacatat ttgcacagtc gattatgcca aaatgaagaa
     1861 atgagggatt tccctcagtt tgacccgatg aaaaaatccc tcattgtctt ggttttacgt
     1921 gcgttttcaa taaatgtgca tgcgttgttc aagatgcaca aagatgtcga ctcatgtcag
     1981 tccgatgtgg aaagatattc cttcaacgcg ctgacgatcc tttcggatgc atggccgtcc
     2041 ccgtaaggat tggaagcttt cgccatggcg ctgtattccg tttcgtcgga tagaagtgtg
     2101 tccgtcaagt tgaagattgt ctcttcgtcc gttccggcaa gtttcaatgt gccggcctcg
     2161 atgccttccg gacgctccgt cgtgtcacgg aggacgatga ccggtttgcc gagcgaaggc
     2221 gcttcttcct ggatgccgcc cgaatcggtc aagatgatat gcgagcgcgc tgcgaagttg
     2281 tggaaatcga cgacttcgag cggttcaatc agatggaccc tgttgttgcc gccgaggatt
     2341 tcgtccgcca cttcgcgaac agccggattc atatggaccg gatagatgac ttgggtgtcc
     2401 g
//
LOCUS       DASULI010000161         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1057777,
            whole genome shotgun sequence.
ACCESSION   DASULI010000161 DASULI010000000
VERSION     DASULI010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057777"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFX34_03475"
     CDS             <1..669
                     /locus_tag="VFX34_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5563997.1"
                     /translation="TTGVILIGVSSYLPTYVTGVMEQSAMIAGFTLTAMSIGWPIASS
                     VAGHLLIRFGTFKVSFAGGISLVAGSLLFVTMTGESGPLWAAFSSFFVGVGMGLTSTS
                     FIVTIQGAVSREQRGSATAANMFMRNFGNTVGAAIFGAVLNGSLMSAFRVKDLDYEVD
                     DINLLLTEASRSALETTKMNSLQQALDGSLQWVYVAVLIFAIISLLLILRIPRGKVVL
                     HDNN"
     gene            656..1546
                     /locus_tag="VFX34_03480"
     CDS             656..1546
                     /locus_tag="VFX34_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5563998.1"
                     /translation="MTTTELEIIKALAEEGNMRKAAERLFLSQPALSQRLQTIEKEWG
                     TMLFVRSQKGLEPTAAGELVIAFAKETILKKEETAEMIASLADKVHGTLKIACASIVG
                     QTWLPQVLKEFVQMYPDAKISLMTGWSSEIVKALYEGEAHVGIVRGQVDWKSRKSYLF
                     RDRLYLVDQEITSINELMDAERPFIQFKSDSNYYMEIQRWWQRHFSQYPRRQITVDQI
                     ETCKQLALNGIGYAILPSITLAGDEKVNKIPLLNSEEEFELTRDTWLIGYDSTFELKQ
                     VSAFTEVLNMHAEKLRKELK"
     gene            1627..>2024
                     /locus_tag="VFX34_03485"
     CDS             1627..>2024
                     /locus_tag="VFX34_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5563999.1"
                     /translation="MWKKQWGIIIAIFVFCLFVPAIHASAAPELKVKVTAGFDGKAKF
                     GKGAPISVEIENSGSSAFTGDMVIDTQQSYESGIGEVFPLDIAAGETKTVTFINQKMF
                     DFNMYGMNSKSIFFYEGGWKKGKEINHKGT"
BASE COUNT          561 a          390 c          543 g          530 t
ORIGIN      
        1 acaacaggcg tcatccttat cggtgtttcc tcgtatttgc ccacgtatgt gacaggtgtc
       61 atggaacagt ctgccatgat tgcagggttt acattgacag ccatgtcgat cgggtggccg
      121 atcgcttctt cggttgcggg ccatttactg ataaggttcg gaacttttaa ggtttcattt
      181 gcagggggca tatcacttgt ggcaggatct ctcttgtttg taacgatgac gggggagtca
      241 gggccattat gggctgcatt ttccagcttt tttgtcggag tcgggatggg attgacgagc
      301 acttccttca tagtaactat ccaaggggcc gtcagccgtg aacaaagggg ttcggcgact
      361 gcagctaaca tgttcatgag aaacttcgga aatacggttg gggcggccat ttttggcgca
      421 gtattaaatg gatcattgat gtcggctttc cgggtgaaag atctggatta tgaagtggat
      481 gatataaatc tgttattgac agaggcatca aggtcggcct tggaaacaac aaagatgaat
      541 agtttacagc aagcgctcga tggctcattg caatgggtat atgtcgctgt actcattttt
      601 gcaatcatca gcttgctgct catattgcgg ataccgaggg gaaaggtggt tctacatgac
      661 aacaactgag ctggaaatca ttaaagcatt ggcggaggaa ggcaacatgc gcaaggcggc
      721 cgaacggcta tttctatctc agcccgcttt gtcccaacgc ctgcagacga ttgaaaagga
      781 atggggaacg atgttgttcg tccgttccca aaaagggctg gagccgactg ctgctggtga
      841 gcttgtcatt gcattcgcga aagagaccat cctgaaaaaa gaggaaaccg ccgagatgat
      901 cgcttctttg gcggataaag tccatggaac ccttaaaatt gcatgcgctt ccattgtcgg
      961 ccaaacatgg cttccgcaag tgctgaaaga attcgtacaa atgtatccgg atgcaaagat
     1021 ttcattgatg acgggatgga gttcggaaat tgtgaaagcc ctttatgaag gggaggcgca
     1081 tgtcgggatt gttcgtggac aggtcgattg gaaaagcagg aaatcgtatt tgttcaggga
     1141 ccgcctctat ttggtcgatc aggaaattac gtccatcaat gaattgatgg atgctgagcg
     1201 gccgttcatt caatttaaaa gtgattccaa ttattatatg gagatccagc gatggtggca
     1261 gcgtcacttt tcacagtacc cgagaaggca aattacagtc gaccagattg aaacatgtaa
     1321 gcagcttgct ttgaatggaa tcggctatgc gattttaccg tccattacat tggcgggtga
     1381 tgagaaagtg aataaaattc ctttgttgaa cagtgaggag gaatttgaac tgacgaggga
     1441 cacatggctg atcggctatg attccacttt tgaattgaaa caagtgagcg cctttactga
     1501 ggtacttaac atgcatgccg aaaaattaag aaaagaattg aagtgaaggg aactttccta
     1561 attttgctac gtactaatta ggaaaggtat acagaatatt ggcggtgtcc aataggggga
     1621 atcaacatgt ggaagaagca atggggaatc ataatcgcca tatttgtctt ttgcttattc
     1681 gtgccggcga tccatgcgtc cgctgcgcct gaactaaaag tgaaggtgac cgccggcttt
     1741 gacggaaaag cgaaattcgg caaaggggcg ccgatttcag tcgaaatcga aaatagcgga
     1801 tcgtccgcat ttaccggtga catggtcatt gatacacaac aatcgtatga atctggcata
     1861 ggagaagttt ttccgctgga tatcgctgca ggtgaaacga aaaccgtgac gttcatcaat
     1921 cagaaaatgt tcgatttcaa tatgtatggg atgaacagca aatccatctt tttttatgag
     1981 ggaggatgga aaaagggcaa ggaaatcaat cataaaggaa ccca
//
LOCUS       DASULI010000162         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1832468,
            whole genome shotgun sequence.
ACCESSION   DASULI010000162 DASULI010000000
VERSION     DASULI010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832468"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..981)
                     /locus_tag="VFX34_03490"
     CDS             complement(88..981)
                     /locus_tag="VFX34_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045232 - S-layer organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system S-layer assembly protein"
                     /protein_id="HEX5564000.1"
                     /translation="MKLFNLFKKTEKTGSDSTIDSEEILEGSAQSEGTDEVETTLSLH
                     PQWALSQEQEYVFRFLSNELEPLKPNQISLSGIDIDVEQANGNWLVKAFFRSSLDQPI
                     TIGPVELLLLDENGEVIASQEFDLNELGELPARSARPWVFVFEKSNTFVEQPPKENWK
                     LAFNVQSMAPHKLELDKAWEEGLSAEQKDALAKVVEQLPKLKPREVNFAGFQVKQQDD
                     KSISVSLFIRNGHSKQISVETLPLELLDATGDIVARGSFNLGPLEVKANTSKPWTFIY
                     PKEMVQKDNPDFSKWIIRVPQ"
     gene            complement(984..>2457)
                     /gene="secA2"
                     /locus_tag="VFX34_03495"
     CDS             complement(984..>2457)
                     /gene="secA2"
                     /locus_tag="VFX34_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292886.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, especially gram positive
                     pathogens, have paralogs that have been found to be
                     nonessential but do function in secretion of a subset of
                     exported proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEX5564001.1"
                     /translation="YVIFKRDVDYIVKDEKVELVDMFTGRIMEGRTLSDGLHQAIEAK
                     EGLPITDENKAQAQITIQNYFRMYPKLSGMTGTAKTQEKEFREVYNMEVIQIPTNRPR
                     VRVDSPDRVYQTIDQKYEAVAKEVAERHRKGQPVLVGTTSILQSEKVAEYLDRENLDY
                     HLLNAKSVEQEVDLISQAGQMGHITVATNMAGRGTDIMLGEGVEEIGGLFVLGTEKHE
                     SRRIDNQLRGRSGRQGDHGESQFFISLEDDMFKRFAKDDLEKFNKKVVVDEDGLVQNS
                     DVNELTERTQRIVEGAHFSMREYNLKLDDVINDQRGVLYSLRDKVLERENLLDQLKTM
                     VSEAVEFVVYDSCPEHETPDNFDFDRIERTMDGLLLEPAALPRNLEKPADILKFYEEP
                     VADLFAYLETFAENEQVNEIIPQVMLSHIDTMWVKHLEVMARLKEGIGLRSYGQEDPM
                     RIYQREGLQLFGTHYQKLRRNIASEVIGFMKLISQQQEAQ"
BASE COUNT          561 a          630 c          553 g          713 t
ORIGIN      
        1 gaatctgctt tttgattgtt atttttctgc tcatccgcca aaaaaagaag cctcgacatg
       61 gtggtcgaag cttcttttct gtttttatta ttgggggact cggataatcc acttggagaa
      121 atctggatta tctttttgaa ccatttcttt cggataaatg aatgtccaag gtttcgatgt
      181 atttgccttc acttcaagtg gtcctagatt gaacgaacca cgtgcaacaa tatcgccagt
      241 ggcatccaac aattcaagcg gcaacgtttc gacactgatc tgcttggagt gaccgttacg
      301 gatgaaaagg gacactgaaa tgcttttgtc atcctgctgc tttacttgaa aaccggcgaa
      361 gttgacttcg cgcggcttta atttaggcag ctgttcaacg actttagcga gtgcgtcttt
      421 ctgttcagcc gaaagtccct cttcccacgc cttgtcaagt tcaagtttat gcggtgccat
      481 ggactgaaca ttgaatgcaa gcttccaatt ttccttggga ggctgctcta caaacgtatt
      541 cgatttctcg aaaacgaaca cccaaggacg tgcgctacgg gccgggagct cgcctaattc
      601 gttcaaatcg aattcctggg aagcgatcac ttcgccattt tcatccaaca gcaataattc
      661 aacgggtcca atcgtgatcg gctgatccag tgaagacctg aaaaacgctt tgaccaacca
      721 gtttccattt gcctgttcga catcgatgtc gattccggaa agggaaatct ggttcggctt
      781 caaaggctcc aattcatttg aaaggaaacg gaaaacgtat tcctgctctt gtgaaagcgc
      841 ccactgggga tgaagggaaa gagttgtttc cacttcatct gtaccttccg actgggcact
      901 gccttccaaa atctcttcag aatcgattgt gctatctgaa cccgtttttt cggttttctt
      961 gaataaatta aatagtttca tgattattgg gcctcctgtt gttgtgaaat aagcttcata
     1021 aagccgataa cttcggatgc gatattccga cgaagcttct gatagtgcgt gccgaataat
     1081 tgcaggcctt cacgctggta gatgcgcatc ggatcttctt ggccgtatga tcgcaatccg
     1141 atcccttcct tgaggcgcgc catcacctcc agatgtttca cccacatcgt gtcgatatgg
     1201 ctcaacatca cttgcgggat gatctcgttc acctgttcat tttcagcgaa cgtttcaagg
     1261 tacgcaaaca ggtcggcaac cggctcttca taaaacttca atatgtcagc aggcttctcc
     1321 aaattgcgtg gaagcgcggc cggctccaat aataatccgt ccatcgtgcg ttcgatccga
     1381 tcgaaatcga agttatccgg ggtttcgtgt tccgggcagc tgtcatagac gacgaattcc
     1441 accgcttcgg acaccattgt tttcaattga tcaagaaggt tttcgcgctc aagcacttta
     1501 tcccgcagcg aatagaggac gccgcgctga tcgttgatga catcatcgag tttcaaattg
     1561 tattcacgca tggagaagtg ggcgccttca acaatccgct gcgtccgttc agtaagttcg
     1621 ttcacatcgg aattttgtac gagcccatct tcatccacga caactttttt gttgaatttc
     1681 tctagatcat ccttcgcgaa acgcttgaac atgtcatctt caagcgagat gaagaattgg
     1741 ctttctccat gatccccttg acgtccggaa cgtccgcgca gttggttgtc gatccgacgg
     1801 ctttcgtgct tttccgtacc aaggacgaat aggccgccga tttcctcgac cccttcaccg
     1861 agcatgatgt ccgttcctcg tcccgccata ttcgtcgcaa ctgtaatatg gcccatttgt
     1921 cccgcctggg aaatgagatc cacttcctgc tcgacacttt ttgcgttcaa tagatgatag
     1981 tcaagattct cgcggtccaa atactccgca accttttcag attggaggat ggaagtcgtt
     2041 ccgacgagga ccggttggcc ttttctatgg cgctccgcca cttctttcgc aaccgcttcg
     2101 tatttttgat cgatcgtctg atagacacga tccggagagt cgacgcggac ccgaggacgg
     2161 ttcgtcggga tctggatgac ttccatgttg tatacttcac ggaattcctt ctcctgtgtc
     2221 ttcgctgttc ccgtcatgcc gctaagcttc ggatacatcc ggaaatagtt ctggatcgta
     2281 atttgcgctt gtgctttatt ctcatcagtg atcggcaatc cttccttcgc ctcgatcgcc
     2341 tggtggagtc cgtccgaaag tgtacgtcct tccatgatcc ggcccgtgaa catatcgaca
     2401 agctcgacct tttcatcttt cacgatgtag tcgacatcgc gtttgaagat gacatag
//
LOCUS       DASULI010000163        11032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1889185,
            whole genome shotgun sequence.
ACCESSION   DASULI010000163 DASULI010000000
VERSION     DASULI010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 11032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11032
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889185"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..982)
                     /gene="cysK"
                     /locus_tag="VFX34_03500"
     CDS             complement(56..982)
                     /gene="cysK"
                     /locus_tag="VFX34_03500"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5564002.1"
                     /translation="MSRVGNTIADLVGKTPLVKLNRMASPDSADIYLKLEYFNPGSSV
                     KDRIALAMIEAAEQSGELKEGSTIIEPTSGNTGIGLAMIAAAKGYKAVLVMPDTMSME
                     RRNLLRAYGADLVLTPGSEGMKGAIGKAEELAEKNGWFMPQQFNNEANPEVHRLTTGP
                     EIADALDRVDAFISGIGTGGTITGAGSVLKERFPDVKIIAVEPTDSAVLSGGKPGPHK
                     IQGIGAGFVPKVLDTEIYDEIIQVTNDEAYDTARRAAREEGILGGVSSGAAIFAALQV
                     AEQLGKGKKVVAILPSNGERYLSTPLYHFEEE"
     gene            complement(1161..2084)
                     /locus_tag="VFX34_03505"
     CDS             complement(1161..2084)
                     /locus_tag="VFX34_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5564003.1"
                     /translation="MKYGQDRNVQETGSQKRRLKTKPLLVLIGILLASNILWLIGWLI
                     PDKSKQPDEEVASVAGVAITRGQWMTAMEKEVGREVLLDLVNNKVMETAAKKYGIKVA
                     DEEIELELALITSVDGRSHSGLDIEQTRQKIRSNLILEKVLSNDVVIDDASVKSFYDK
                     NESLYNILTAYRTSVIIVPTKEEAEQAIDELSKGSSFDALAKELSTDLASASLGGDVG
                     YINAETETIDPAIYKAASKQKEGKVGDVVELQDGTYAIILVNEVVEGRAFSFDEVKEH
                     IRRELAIEQLPQSVSPEAFWKEFDAKWFYGK"
     gene            complement(2062..2949)
                     /gene="hslO"
                     /locus_tag="VFX34_03510"
     CDS             complement(2062..2949)
                     /gene="hslO"
                     /locus_tag="VFX34_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX5564004.1"
                     /translation="MADYLIKALAFEGTIRAYAVNSTNAVGEAQKKHHVWPTATAALG
                     RTMTAGLMMGAMLKGDDKLTVKIEGDGPAGPIVIDADARGDVRGYITNPQTHFDLNEQ
                     GKLDVRRAVGTEGMLTVVKDLGLRDFFTGQVPIVSGEIAEDFTQYFVVSEQVPSAVAL
                     GVLVNPDNTVKAAGGFIIQVMPGATEETISLLEQKLANVEPISRMIDRGLTPEEVLGE
                     VLGKDNVEILEKMDVAFRCNCSKERFGNAIIGLGEQEIREMIDEDGQAEAECHFCLEK
                     YLFTKEELEGFIDEIRSRS"
     gene            complement(2965..3729)
                     /locus_tag="VFX34_03515"
     CDS             complement(2965..3729)
                     /locus_tag="VFX34_03515"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5564005.1"
                     /translation="MILVLDTGNTNIVLGVYDKGELKYHWRMETYRQRTEDEYAMSVK
                     ALFTHVGLKFEDINGIIISSVVPPVMFPLEQMCRKYFNLRPLIVGPGVKTGLNIKYEN
                     PREVGADRIVNAVAAIHDYGSPLIIVDFGTATTFCYVNEKGEYMGGSIAPGIGISMEA
                     LFDRASKLPRVELTRPDHIIGKNTVAAMQAGIVYGYVGLVDGIVGRMKEHSKVEPTVI
                     ATGGLAELIASETQVIDIVDNFLTLKGLHLIYERNV"
     gene            complement(4031..6058)
                     /gene="ftsH"
                     /locus_tag="VFX34_03520"
     CDS             complement(4031..6058)
                     /gene="ftsH"
                     /locus_tag="VFX34_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5564006.1"
                     /translation="MNRILRYFLLYGLIFLAIMGIFSSLNNPNPKTKEIRYDEFYTSL
                     EKGDIETLSFQPVRDVLTVEGTMKGYEEGEKFTTNVLMNDTSVMEVIRNIEATGKTNN
                     PQIQIIPAPETSAWVAFFTGLVPFIIIIILFFFLLNQAQGGGGGRVMNFGKSKAKLHS
                     DDRKKVRFTDVAGADEEKAELEEVVDFLKDSRKFAELGARIPKGILLVGPPGTGKTLL
                     ARAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFENAKKNAPCIIFIDEIDAVGRQ
                     RGAGLGGGHDEREQTLNQLLVEMDGFDGNEGIIIVAATNRPDILDPALLRPGRFDRQI
                     TVGRPDVRGREAVLKVHARNKPLDESVNMKALAQRTPGFSGADLENLLNEAALVAARR
                     GKAKIDMADIDEATDRVIAGPAKTSRVISEKERNIVAFHEAGHVVVGLMLDDAEIVHK
                     VTIVPRGQAGGYAVMLPKEDRYFMTKPELLDKIAGLLGGRVAEEIVLGEVSTGAHNDF
                     QRATGIARSMVTEYGMSEKLGPMQFGQAQGGNVFLGRDFNSEQNYSESIAYEIDQEMQ
                     RIIKDQYERTTKILTEHRNLLDLIATTLLEVETLDAEQINHLKDHGTLPERPYEQNGN
                     GSQKKSDDADAEKDTVHSDSTGAPADPSIGDLPKEKDVDQSLPPIDEHRRD"
     gene            complement(6283..6825)
                     /gene="hpt"
                     /locus_tag="VFX34_03525"
     CDS             complement(6283..6825)
                     /gene="hpt"
                     /locus_tag="VFX34_03525"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5564007.1"
                     /translation="MLQNDIQEVLITEEEIQEKIAELGAVLTEDYQDKFPLAIGVLKG
                     ALPFMSDLIKRIDAYIELDFMDVSSYGNATVSSGEVKIVKDLNASVEGRDVLIIEDII
                     DSGKTLNYLVELFKYRKAKSIKIVTLLDKPTGRKVDLKADYVGFLVPDAFVVGYGLDY
                     AEKYRNLPYIGVLKKEIYSF"
     gene            complement(6855..8240)
                     /gene="tilS"
                     /locus_tag="VFX34_03530"
     CDS             complement(6855..8240)
                     /gene="tilS"
                     /locus_tag="VFX34_03530"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5564008.1"
                     /translation="MHVFERKILDFIHKEALLSPDHRVLVACSGGVDSVALLLFMAIM
                     REKLQIEVAAAHVDHMLRGKESADDGKFVKGLCEKLGLPFFGGNVPVPSILSKEGGNV
                     QDVCRSGRYTFFDEVMRAEGYTILATAHHAEDQLETVLIQLSKGSAPLGVPVSRTIDG
                     GTVIRPFLPAMKGELLAYVEEQGAQFREDPSNESDSYLRNRLRHQVAPIILSENPSAA
                     LNVVKMTTGLQEDEMLLDSLVKERFESIITFHEDGLPTFELDLFRSMHTALQKRFIPL
                     LLNYLYSGEIIPVEYNTALLNQLHHHLSASEGNITIDLPKGYRFVREYGTVSIVKNAG
                     ISDASIRKLQKGEWTSWGDMLLYWDDADGHDEELAELMYFDLPDSDLPLYVRGREDGD
                     RIMLPGMAQPKRLSRLFIDEKIGAEKRRKLPVVTTAHGEVCAIPGVRYGIRFRKHKTE
                     RETYIFKMKEK"
     gene            complement(8282..10693)
                     /locus_tag="VFX34_03535"
     CDS             complement(8282..10693)
                     /locus_tag="VFX34_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX5564009.1"
                     /translation="MKMIGNLERPVGQQIRHVFSAVGRRKMYVLVAAFFLLSTFFLSQ
                     AVLFNAAVPFFLPIWALAQMRFRNHLIYVFIGGIAGGAFLGLGQAVIHLLQLLLFNVV
                     SKHPLMRKSIPLTVAGTIIVVQVLWQFVMHSGHTPVEVQLSIGFEAVLALFMTFFLFV
                     AFPHRERIFFGQWSPERLGAVCIVGIMATTGMGDLMVGPISISGLLLHLTILLAALAG
                     GLPFSTTIAMMIAAIAGVAELSFTGMMAVYGMTGFFAGALKRLGKLGIATGGLAVSVF
                     FLLYDFTLPLDTSHFLTIGLATLLFFFIPTKKVEPIGRMITPSAQQDLSVKRQQWLTD
                     RLDEQLSDFQQFAEFMSNLVNDRFASDDGNAVQNIPSICQSCFRYSKCWEGNEEGMAR
                     LLYEWEGTYSATKKAARHRVEERIKYKCIRSSGLISELEESASNHLLMGQLQHGRKML
                     ALQLRDMSNHLEKIMNDIKGELSVNHLAEEELGKHLQSQGIEYYQIDILSEEKGAYRI
                     VVSIPERRSDFETDTTVAERLILPLLEEVYREPFKVEKTAVLQDPFPHIQVMFCSAVR
                     FSLEYGIVATAGGGAFHAGDAYEVFPIHDGLTAVLLSDGMGHDINAYRESRKVIRLMR
                     ECLDRKMDPETAMHTLHYMMSLNGLDDMYATLDLALIDLQDGKLWSWKAGSMSTYIKR
                     GQDFLRLDSKSVPVGFLPTFSVEATNEELKSGDVIVMLTDGVFNGSVTIEKQEDAIYG
                     ILEKYEHLSSEPLADRIMSEMERKFGIVADDRTVLVMKVEHVLPKWTTIKPTNRFISR
                     EKVMG"
     gene            10890..10963
                     /locus_tag="VFX34_03540"
     tRNA            10890..10963
                     /locus_tag="VFX34_03540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10924..10926,aa:Met,seq:cat)
BASE COUNT         2690 a         3070 c         2306 g         2966 t
ORIGIN      
        1 ctagttttag aacgaaaaaa gagagccttg tcagctctcc ttcaacgaac atctcttatt
       61 cttcttcaaa atgataaagc ggggtgctca agtaacgctc gccgttcgac ggaaggatgg
      121 caacgacctt tttgcccttc cctagctgtt ctgcaacttg gagtgctgcg aaaatagctg
      181 ccccggaaga gacgccgccg aggattccct cttcccgtgc tgctcttctg gccgtatcat
      241 acgcttcgtc attcgttact tggataattt catcgtaaat ctccgtatct agcactttcg
      301 gaacgaagcc ggcgccgatc ccttggattt tatgcggacc cggctttcca cccgacaaga
      361 ctgccgaatc ggtcggttcc actgcgatga ttttcacatc cgggaatcgc tctttcaata
      421 cactgcccgc gccggtgatc gtgcctcccg tacctatgcc cgatatgaac gcgtcgacac
      481 ggtcgagcgc atctgcaatt tccggtcctg tcgtcagacg gtgcacttca ggattcgctt
      541 cgttattgaa ctgttgcggc atgaaccagc cgttcttctc ggcaagttct tcagcttttc
      601 caatggcgcc cttcatccct tccgatcccg gagtcaagac caggtccgct ccataagcgc
      661 gcaagaggtt ccggcgctcc atgctcatcg tatccggcat gactaggacc gccttatatc
      721 cttttgctgc agcgatcatc gccaggccga tgcccgtatt cccgctcgtc ggctcaatga
      781 tcgtgcttcc ttccttcaat tccccggatt gttcagcagc ttcgatcatc gctaatgcaa
      841 tcctgtcttt tacgcttgat cccggattga agtattccag cttcagataa atgtccgcgc
      901 tgtcagggct cgccatacgg ttcaacttga cgagaggcgt ttttccaaca aggtctgcta
      961 ttgtgtttcc gactctactc attcctaccc actcctaatc cctacttatt tgatatgttt
     1021 tattgtatta atagtacacc tggattgtga acagtgtcaa tcaataatgc ctcctttaat
     1081 ctttccctct ttccaaatgt ataatctgaa aaagctgttc agaaccactc ctttatcggg
     1141 aggcctgaac agctttcatt tcattttcca tagaaccatt ttgcatcgaa ttctttccag
     1201 aaagcttcgg gactgaccga ttgcgggagc tgttcgatag caagttcacg cctgatatgc
     1261 tccttcactt catcaaagga aaacgctctg ccttcaacca cttcattcac tagaatgata
     1321 gcgtatgtac cgtcctgcag ttcaacaacg tccccgacct tgccttcctt ctgcttggat
     1381 gcagctttat agattgcagg gtcaatcgtc tccgtttcgg cgtttatata gccgacgtca
     1441 ccaccgaggc ttgccgacgc aagatcggtc gacagctctt tcgccaaagc atcgaagctc
     1501 gaccctttcg acaactcatc aatcgcctgc tccgcctctt cttttgtcgg aacgatgatg
     1561 acggaagttc gatatgcggt cagtatatta tagagcgatt cgtttttatc ataaaaggat
     1621 ttcacggagg catcgtcaat gactacgtca ttcgacaata ctttctccaa tatcagattt
     1681 gaccggattt tttgccgcgt ttgttcaata tccaaacccg agtgcgaacg gccgtccacc
     1741 gaagtgatta atgcgagctc aagttcgatt tcctcatcgg ccactttgat gccgtatttc
     1801 tttgcagccg tctccatcac cttattgttg acgagatcga gaagcacttc cctgccgact
     1861 tccttttcca tcgccgtcat ccactgcccc ctcgtaatcg ccacgcccgc aaccgaagca
     1921 acctcctcgt ccggttgctt cgatttgtcg gggatgagcc atccgatgag ccaaaggatg
     1981 ttactagcga gaaggattcc gatcaagaca agaagcggct tcgttttcaa tctccttttt
     2041 tgagagcccg tctcctggac gttacgatct tgaccgtatt tcatcgataa atccttcaag
     2101 ttcttctttc gtgaataaat acttctccaa acagaagtgg cactccgctt ccgcttgtcc
     2161 atcctcatca atcatttccc ggatttcctg ctctcctaga ccgatgatcg cattgccgaa
     2221 acgttccttc gagcaattgc aacggaatgc aacgtccatc ttttcgagga tttcaacatt
     2281 gtctttgccc aaaacttctc caagcacttc ttcaggcgtc aggcctcggt caatcatcct
     2341 ggaaatcggt tcaacatttg caagtttctg ctcaagcagg ctgatcgtct cttccgttgc
     2401 acccggcatc acttgaatga tgaagccgcc tgcagctttg acagtattgt ccggattgac
     2461 aaggacgccc aatgcgaccg ccgaagggac ctgttccgac acgacgaaat attgtgtgaa
     2521 gtcttccgcg atttcccctg aaacaatcgg aacttgccct gtgaaaaagt cccgtaaccc
     2581 aaggtctttc acaactgtca gcattccttc cgtaccgacc gcacgtctga cgtcaagctt
     2641 cccttgttca ttcaaatcaa agtgggtttg cggattcgta atatatcctc ttacatcacc
     2701 gcgcgcatca gcatcaatga caatcggtcc agccgggccg tccccttcga ttttcaccgt
     2761 cagtttgtca tcgcctttca gcattgcacc catcatgagg cccgctgtca tcgtccggcc
     2821 caaagccgct gtagctgtcg gccaaacgtg atgctttttc tgcgcctcac ccactgcgtt
     2881 tgtcgaattc accgcatatg cgcgaatcgt cccttcaaaa gccaatgctt ttatcaaata
     2941 atctgccatt catgtctact cccttcaaac atttctttcg taaattaaat ggagcccctt
     3001 caacgtaagg aaattatcta cgatatctat cacttgcgtc tcactggcaa tcaattcggc
     3061 aaggccgcca gtcgcaatga cagtcggctc caccttactg tgctccttca tcctgccaac
     3121 gataccatcg acgagcccga catacccata aacgatgcct gcttgcatcg cggctacagt
     3181 atttttgccg ataatatggt ccggcctcgt caattcgaca cggggcaatt tcgacgcccg
     3241 gtcgaataat gcctccatgg agatgccgat gccaggggca atcgaacctc ccatatactc
     3301 acctttttca ttcacataac agaatgtcgt cgcagttccg aaatcaacga tgatgagcgg
     3361 tgagccataa tcatggatcg ccgcaaccgc attgacgata cggtccgctc caacttcacg
     3421 gggattttca tattttatat tcaaacctgt tttgacgcca ggaccgacga taagcggacg
     3481 caagttgaaa tacttcctgc acatctgctc cagcgggaac atgaccggcg gaacgacaga
     3541 ggaaatgatg atgccgttga tatcttcaaa tttgaggccg acatgggtga aaagcgcttt
     3601 cacggacatg gcatattcat cttccgttct ttgcctatat gtctccatgc gccaatggta
     3661 tttcaattct cctttatcat aaacgccaag aacgatattc gtatttcccg tatccaatac
     3721 tagaatcatt ttcttttcct cgctaacatt cgtcattttc caaaacatca taacaaaagc
     3781 ggaaggtggc gtctagccaa tcaaacagcg tagggtttga tttgccgtat ttctgcgcac
     3841 tttgcagaaa ttaaggcatc ccctcaaccg atgctcgtaa cgcttcgctt ttactcgcaa
     3901 aagccgttct ccgttacggc tttcgctgcc gcccgcagct ggacgatacc atattggata
     3961 gaaataaaaa cagtcgactg ccttaaaaag cagtcgactg gaatgttatc tttcaagttg
     4021 caccgttacg tcaatctctt ctatgctcat cgattggagg aagggattga tctacatcct
     4081 tctccttcgg cagatctccg atggatggat ctgctggcgc gcctgttgaa tccgaatgta
     4141 cagtgtcttt ttcagcatct gcatcatccg atttcttttg tgagccattt ccattttgtt
     4201 catatggacg ctccggcaaa gtgccatgat ctttcaagtg gttgatctgc tcagcatcca
     4261 acgtttccac ttcgagaagc gtcgttgcga tcaagtcgag caagttgcgg tgctccgtca
     4321 ggatcttcgt tgtgcgctca tattgatctt taatgatgcg ctgcatttcc tggtcgattt
     4381 catacgcaat cgattcggag tagttctgct cggaattgaa gtctctgcca aggaaaacgt
     4441 tgccgccttg cgcctggccg aattgcatcg gtccgagctt ttcgctcata ccgtattccg
     4501 tcaccatgga acgggcaatg cctgtagcgc gctggaagtc attatgggct cccgtggata
     4561 cttccccaag aacgatctct tccgcaacac gccctccgag gagtccggcg attttgtcga
     4621 gaagttccgg tttcgtcatg aagtaacggt cctctttcgg cagcatgacc gcatatccgc
     4681 ccgcctggcc gcgaggaacg atcgtcactt tatggacgat ttcggcgtca tccaacatga
     4741 gtccgacgac aacgtggccc gcttcgtgga acgcgacgat attcctttcc ttttcggaaa
     4801 tgacccggct cgttttagcc ggtcccgcaa tgacgcggtc cgtcgcttca tcgatatccg
     4861 ccatgtcgat tttcgctttg ccgcgtctag ctgcgaccaa tgcagcttcg ttcagcaagt
     4921 tctctaggtc ggcacccgag aatcccggag tgcgttgtgc caaagccttc atattgactg
     4981 attcgtcaag cggcttattg cgcgcatgca ctttaaggac tgcttcacgg cctcttacat
     5041 ccggacggcc gacagtgatc tgacggtcga atcgtcctgg acgaagaagt gcagggtcta
     5101 gaatatccgg acggttcgtc gcggcaacga taatgattcc ttcgtttcca tcgaacccat
     5161 ccatttcaac caatagctgg ttcaacgtct gttcacgctc gtcatgtccg ccaccgagac
     5221 cagcgccccg ttgacgtccg actgcgtcaa tttcatcgat gaagatgata catggcgcat
     5281 tctttttcgc gttttcaaac aagtcacgga cacgtgatgc cccgacccca acgaacattt
     5341 caacgaagtc cgaaccggag atcgagaaga atggcacgcc ggcttcaccc gcaacggctc
     5401 gcgccaaaag cgtcttacct gtacccggag gtccgaccaa aaggataccc tttggaatac
     5461 gtgctccaag ttcagcaaac ttacgagaat ctttcaagaa atccacaact tcttcaagct
     5521 cggctttctc ttcatccgca cccgcaacgt ctgtgaatct gacttttttg cgatcatcgg
     5581 aatggagctt cgccttgctt ttcccaaaat tcatgacccg gccgccaccg ccgccttgcg
     5641 cttgattcaa taggaagaag aataggatga tgatgataat gaatggaacc aatcctgtga
     5701 agaaggctac ccatgcactc gtttctggag caggtatgat ttggatctga gggttattcg
     5761 ttttaccggt agcttcgata ttgcgaatca cttccataac agaagtatcg ttcatcaaaa
     5821 cattcgtcgt gaatttttct ccctcttcat agcccttcat cgtaccttca acagtcagaa
     5881 catcacgcac tggttgaaac gataacgtct caatatcgcc tttttccaac gatgtataaa
     5941 attcatcata ccgaatctct ttcgttttcg gatttggatt atttagtgaa ctgaaaatcc
     6001 ccataattgc aaggaagatc aatccgtata gtagaaagta tcgaagtata cgattcatcc
     6061 caagcctcct catttctttc aacagaaaac tattgtaaat cttatcatat aacagattca
     6121 acctacaaag gaaaagcctt tcttgctaag gtataaatta cggattcacc cttcagccac
     6181 attagcaaac cgcactacga cagaggctga tagtatgcca acttccggtt taatatggaa
     6241 aaagctttcc atcgcccctc gtaaaggttt gttgaaaagc tttcagaaag agtagatttc
     6301 ctttttcaat acaccgatat aaggcaagtt cctatatttt tctgcgtaat caagcccata
     6361 accgacgacg aaagcatccg gtactaggaa tccgacataa tccgctttca aatccacttt
     6421 tcggcccgtc ggtttgtcaa gcaacgtgac gattttaatg gatttcgctt tccggtattt
     6481 gaaaagttcg actaagtaat tcaatgtttt accgctatca atgatgtctt cgatgatcaa
     6541 tacgtcgcgg ccttccacac ttgcattcaa gtctttgacg attttcactt ctccggatga
     6601 cactgtcgca tttccatagc tggatacatc catgaaatcc aattcgatat acgcatcgat
     6661 tcgtttgatc aaatcgctca tgaacggcaa cgctccctta aggacaccga tcgcaagcgg
     6721 gaatttatcc tggtagtcct ccgtcagcac ggcacccaat tccgctattt tctcttggat
     6781 ctcttcttct gtgatgagca cttcttgaat atcgttttgc aacatcgagt tccctctctt
     6841 ttccgttaag attttcactt ttccttcatt ttgaatatgt acgtttcccg ttccgtttta
     6901 tgtttcctga accggatgcc atatcgtacg ccaggaatgg cgcaaacttc accgtgcgca
     6961 gttgtcacaa ccggcagctt ccgccgtttt tcggcgccaa ttttttcatc aatgaacaat
     7021 ctggacaaac gcttcggctg ggccatgccc ggaagcataa ttcggtcccc atcttcccgg
     7081 cctcttacgt aaagcggcaa atcggaatcg ggcaaatcga aatacatcaa ttcggcaagt
     7141 tcctcgtcat gtccgtccgc atcgtcccaa tataagagca tatctcccca gcttgtccat
     7201 tcgccttttt gcagcttccg aatggaagca tcactgattc cggcattttt cacaatcgaa
     7261 acggtgccgt attcccgaac gaaccggtac cctttcggca ggtcgatcgt gatatttccc
     7321 tcacttgcgg acaagtggtg atgcaattgg ttcaataggg ctgtgttgta ttctacaggt
     7381 atgatttccc cggagtatag ataattcaat agtagtggaa taaagcgctt ttgtaaagca
     7441 gtgtgcatgc ttctgaacag atccaattca aaggtcggca gcccgtcttc atggaacgta
     7501 ataatcgact caaaacgctc ttttacaagg ctgtccagca gcatttcatc ctcctgcagc
     7561 cccgtagtca ttttgacgac attcaaggct gcggatggat tttccgacaa gatgatcggc
     7621 gctacttgat gacgcagccg attccgcaaa taggagtcgc tttcgttact cggatcctcc
     7681 ctgaattgcg ctccctgctc ctccacatac gcaagaagct ctcccttcat tgccggaagg
     7741 aaaggtctga tgacggtgcc gccatcgatt gtccggctta ccgggacgcc tagaggagcg
     7801 cttcccttcg acaattgaat gagcaccgtc tccaattgat cctcggcatg gtgggcggtt
     7861 gccagaatag tatagccttc cgccctcatg acctcatcaa aaaaggtata tcttccagat
     7921 ctgcagacgt cctgcacatt cccgccttcc ttgcttagga tggatggaac gggcacattc
     7981 ccgccgaaga aaggaagtcc gagcttctca cataatccct tcacaaattt gccgtcatcg
     8041 gcggattctt ttcctcgcag catatgatcc acatgggcgg cagccacttc aatctgcagt
     8101 ttttcgcgca ttattgccat gaagagcaaa agtgcgactg aatccacccc gcccgaacat
     8161 gcgaccaata cccggtgatc cggtgacagc aatgcctctt tatgaatgaa atcaaggatt
     8221 ttcctttcga atacatgcat attgcgcccc tccgtctgtt atcttctgat ttcctcctat
     8281 tctaccccat aactttttcc cgggaaataa atcgatttgt gggctttatt gtcgtccatt
     8341 tcggtaaaac gtgttcaact ttcattacca atacagttcg atcgtccgca acgatgccga
     8401 acttcctttc catttcggac ataatacggt cggctaaagg ctcgctggac agatgctcgt
     8461 acttttccaa tatcccatag attgcgtctt cctgcttttc aatcgtcacc gaaccgttga
     8521 atacgccatc tgtcaacatg acgataacgt cacccgattt caattcttca tttgtcgctt
     8581 ccacggaaaa cgtcgggagg aatccgacag gaaccgattt gctgtcaagc cggagaaagt
     8641 cctggcccct ctttatatag gtactcatcg aacccgcctt ccaagaccag agctttccat
     8701 cctgcaagtc gatcaaagcc aaatcgagcg ttgcatacat atcatcaagc ccattcaatg
     8761 acatcatata atgcaatgta tgcatcgctg tttcgggatc cattttccgg tccaggcatt
     8821 ccctcatgag acggatcact ttccggcttt cccgataggc atttatatca tgccccattc
     8881 cgtccgacag tagtacagcg gttaggccgt catgaatcgg aaacacttca taggcgtctc
     8941 ccgcatggaa cgcccctccc ccagccgttg ccacaatccc gtattccaat gaaaagcgga
     9001 cagccgaaca gaacatgact tgaatatgcg ggaaagggtc ttgcagcacg gccgtcttct
     9061 ccactttaaa cggctcccga tagacttcct ccaacagcgg caatatgagc cgttccgcca
     9121 cggtcgtatc cgtttcgaag tcggaacgtc tttcagggat ggaaacaacg atccggtagg
     9181 cgcctttctc ctccgataaa atgtcgatct gataatattc gattccttgt gactgcaaat
     9241 gctttcccaa ttcctcctct gcaagatggt tgacagacaa ctcccctttg atatcattca
     9301 tgatcttctc caaatggttg ctcatatccc tcaattgcaa tgcaagcatt ttccgaccgt
     9361 gctgaagctg ccccataaga agatgattgg aagccgattc ctccagttcg gaaatcaaac
     9421 ctgaagagcg gatgcactta tatttaattc gttcctcgac acggtggcgt gccgctttct
     9481 ttgtcgctga atacgtccct tcccactcat aaaggagacg ggccatccct tcttcattcc
     9541 cctcccagca tttcgaatac ctgaaacagg attggcagat cgacgggatg ttctgtacgg
     9601 cattcccatc atccgaagcg aaacggtcat tgacgagatt ggacatgaat tcagcgaact
     9661 gctgaaaatc ggataattgc tcatcaagac gatcggtcag ccattgctgc ctcttcactg
     9721 atagatcctg ttgagcgctg ggtgtgatca tcctaccgat aggttccact ttcttcgtgg
     9781 ggatgaagaa aaacagaagg gttgccaagc cgatcgttaa aaaatgggac gtatcaagcg
     9841 gcaatgtaaa atcatataaa aggaagaaaa cggataccgc caagccgccc gtcgcaatac
     9901 ccaatttccc cagacgcttt aaagcgcctg caaaaaagcc cgtcatgcca tatacggcca
     9961 tcatcccggt aaacgacaat tccgcaactc cagcaattgc ggcgatcatc atcgcaatcg
    10021 tagtggaaaa cggcagaccg cctgcaagcg ctgccaacaa aatggtcaaa tggagaagca
    10081 atcccgaaat tgaaatcggg ccgaccatca gatcccccat cccggttgtc gccataatcc
    10141 cgacaatgca aacagccccg agacgttccg gcgaccattg tccgaaaaag atgcgctccc
    10201 gatgcggaaa tgcgacaaac aggaagaatg tcatgaacag cgccaaaacc gcttcgaaac
    10261 cgatcgacaa ctgcacttcg acaggcgtat gcccgctatg catgacaaac tgccaaagaa
    10321 cctggacgac aatgatcgtc cctgcgaccg tcaaaggaat ggacttccgc atgagcggat
    10381 gcttgctcac gacattgaac aacagtaatt gcaataaatg gatgaccgct tgtcccagcc
    10441 ccagaaaagc ccctcccgca attccgccta tgaatacata gatgagatga ttcctgaatc
    10501 gcatctgtgc caatgcccat atcggcagaa aaaatggaac agctgcgtta aaaagaacag
    10561 cttgcgacaa gaaaaacgta cttagtaaaa agaacgccgc caccaataca tacatcttcc
    10621 gcctgcccac cgcgctgaac acatgacgaa tctgctggcc taccggtctt tcaagattgc
    10681 ctatcatctt catctccaca ttccccctat gtctacttgt ccctgagtat acataggtca
    10741 agccaaagaa tttgtctgtt cctgacaagg gaccccataa atctttcgac gagttccgaa
    10801 gcgtttttct attttacaac gaaaggttga cagaccctgc cgaccgttgt attatgatta
    10861 ttgtcgttca gaaatctttt gaacaacatg gcggcgtagc tcagctggct agagcgttcg
    10921 gttcataccc gaaaggtcgt gggttcgacc ccctccgccg ctataaacga aaagcgtcca
    10981 gtctacagac tggacgcttt ttttattgct gaattgcgca taaatctatg ga
//
LOCUS       DASULI010000164         5804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_216053, whole
            genome shotgun sequence.
ACCESSION   DASULI010000164 DASULI010000000
VERSION     DASULI010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216053"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VFX34_03545"
     CDS             complement(<1..149)
                     /locus_tag="VFX34_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5564010.1"
                     /translation="MTNQPIIRFENVMKKYGDGTTVLDNVSFEMERGKFYTLLGPSGC
                     GKTTIL"
     gene            complement(166..708)
                     /locus_tag="VFX34_03550"
     CDS             complement(166..708)
                     /locus_tag="VFX34_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX5564011.1"
                     /translation="MHIGSKIKSLRLKKGLTQEELGERTDLTKGYISQLERELTSPSI
                     ETLFSLLEVLGTTPKEFFDEPKKNMKVVYSPADQTTYVNDELKYSIRWLVPRSNENEM
                     EPIHITFSKNGEFKQFEPSLAETFIYVLSGKVEIELGNRSFTAIQGDSVYYEASDHHR
                     ISNANDGQSELLLVATESYL"
     gene            complement(865..2325)
                     /locus_tag="VFX34_03555"
     CDS             complement(865..2325)
                     /locus_tag="VFX34_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564012.1"
                     /translation="MPIPMEVEKLNMIRSHMTFILPFGFDQMKRRQTIDSLEANGYTY
                     FHIDEEAKYERAMYGHGIEVVGKELEQYFYPYIEHKLFPGSHERKGFHRYSAPILKGH
                     IMQLRNERYDFYIRSIDIILGPFGIAFLALRIEMEDASADLCDVLDFIQHFRAIEPNL
                     KESKGAMIHIPESGQKFSIHDYLFEHLCPFIKETVLHDKKLKGHFGSLPYFEDERMFV
                     SSFLITETGATISEEQLYRIGNLNGRSPDGAPFISSTNPDYIRRSLAESLHDRWAPHL
                     FMIATEHGFSVVSNRPADELKNELSQFMGTHYYNLLLHYFYKIMLLRVSFEYSEIDWK
                     KDEYYVRSLIQLINVFSSSYYFKEVSTRSEGKELAEMFRSTFLIDPLFNEVSKTLQEL
                     YKSQENSQAGRLNMLLFILTIFTVISGIYGMNLVIPDWEAPSGWKEYPSYTVFEWISL
                     ITAVTGIGLSAYLILSTFGRMYLRRRRRNKFNMHDQ"
     gene            2477..2941
                     /locus_tag="VFX34_03560"
     CDS             2477..2941
                     /locus_tag="VFX34_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5564013.1"
                     /translation="MKLHEQMPELNGATVWLNGRYEKADLIGRSPTLIHFWSISCRLC
                     KESMPYMNNLRSRFKDRLNIIAVHMPRSEEDLNLQQIKKVAAEHNMTHPIFVDGELIL
                     TDAFDNQYVPAYYLFDKKGRLRHYQTERSGMGMLRKRVDRLLNEMDRGDGRI"
     gene            3017..3676
                     /locus_tag="VFX34_03565"
     CDS             3017..3676
                     /locus_tag="VFX34_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX5564014.1"
                     /translation="MKKEFVVIGLGRFGGSIVNELVELSVDVMAIDISPDRVEEFAQI
                     ATTAVAADTTDESALRSLGIRNFEHVVVAIGENIQASILTTLILKEIGVKRITVKAQN
                     DYHAKVLRKIGADQVVHPERDMGKRIANNLVSNNILDYLELSDEYSIAEIRANEKLAG
                     FTLIDLDIRAKYGVNIVAIKREAQILVSPQAIEEIQLNDILIVIGSDLDIHRFEKKAL
                     H"
     gene            complement(3967..5634)
                     /locus_tag="VFX34_03570"
     CDS             complement(3967..5634)
                     /locus_tag="VFX34_03570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5564015.1"
                     /translation="MKLIKNNETAVFALGGLGEIGKNTYCVQFQDEIILIDAGIKFPE
                     DDLLGIDYVIPDYTYLERNVDKIKGLFITHGHEDHIGGIPYLLRKINIPVYGSKLALG
                     LLRNKLDEHGLLRSTKMIEFKDDDVFKFRKTSVSFFRTTHSIPDAFGVVVKTPSGNIV
                     HTGDFKFDFTPVGEPADLAKMAKFGSDGVLCLLSDSTNAEVPNFTMSERKVGDSLNEI
                     FRKVDGRIIFATFASNIHRLQQVIEAAERHGRKIAVFGRSMDNAITIGRELGYINAPK
                     ELFVDTQSLNRLPANEVMILCTGSQGEPMAALSRIANGTHRQVQIQPGDTVIFSSSPI
                     PGNTLSVNKTINALFRAGAEVIHGALNNIHTSGHGSQEEQKLMLRLIKPKYFMPIHGE
                     YRMLTMHKELAIGCDVDPDNIFIMSNGDVLALTEDTARLAGKVPSGDVYIDGSGIGDI
                     GSVVLRDRKILSEDGLVIVVATVDKKRNKVVSGPDIISRGFVYMRESGMMISEAQHLL
                     SKELNKKLANSPAEAGSLKSDIIDILSPYLYDQTKRRPMVLPVVMEV"
     gene            complement(5640..>5804)
                     /locus_tag="VFX34_03575"
     CDS             complement(5640..>5804)
                     /locus_tag="VFX34_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase epsilon subunit"
                     /protein_id="HEX5564016.1"
                     /translation="IRENTKHLYIEADSERSVREKLKNREYNIELIQLLEGNHLEYEQ
                     ASPHFELEQV"
BASE COUNT         1537 a         1280 c         1338 g         1649 t
ORIGIN      
        1 aggattgtcg tctttccgca accggaagga cctaatagtg tgtaaaactt accgcgctcc
       61 atttcaaatg agacattatc caatacggtc gttccgtcgc cgtatttctt cataacgttc
      121 tcaaatcgga taatcggctg atttgtcatt ttggtagttc ccccttcata aataagattc
      181 tgtcgctaca agaagcaatt cgctctggcc gtcgtttgca ttcgatatcc gatgatgatc
      241 ggacgcttcg taatagaccg aatcgccctg tatcgctgta aaagaccggt tccctagctc
      301 gatttccact ttgcccgaaa ggacatagat gaaggtttcg gcaagggatg gttcaaattg
      361 tttgaactcg ccgttcttcg agaatgtaat atggatcggt tccatttcat tttcattcga
      421 acgggggacg agccagcgga tgctatattt cagctcatca ttgacgtagg ttgtttggtc
      481 ggcgggcgag tagacgactt tcatgttttt cttcggttca tcaaaaaatt ccttcggtgt
      541 tgtgcccaac acttccaata gcgaaaagag cgtttcgata gagggggaag tgagttcccg
      601 ctccaattgt gaaatatatc ccttcgtcag gtcggtcctt tccccgagct cctcttgggt
      661 caatcctttt ttcaatcgaa gacttttgat tttactcccg atatgcatgg tcgacaattc
      721 cttcctcgaa tcatggagtt tagtattact gaactttgtg tttactataa gcctatttat
      781 tatacaaaat accacttgga aatcaagatg tttttttaaa aaaatgccgg aatccatttt
      841 caggactccg gcataaagca aaatctattg gtcatgcatg ttaaacttat tccgtcgccg
      901 ccttcgcaag tacatccggc cgaaagtgga aaggatcaaa taagcagata acccgattcc
      961 cgtgacggcg gtaatcaatg aaatccattc gaaaaccgtg taggaggggt attctttcca
     1021 gcctgaaggg gcctcccagt cgggaatgac aaggttcata ccgtaaatac cggaaatcac
     1081 agtgaatatc gtcaaaatga atagaagcat gttcaatcgg ccggcttggc tattttcctg
     1141 gcttttatat aactcttgca acgtcttgct cacctcattg aaaagaggat cgatcagaaa
     1201 ggtactcctg aacatctccg ccaattcttt gccttccgat ctggtgctga cttccttgaa
     1261 ataatatgag gatgaaaaaa cattgatgag ctggatgagt gacctgacgt aatattcatc
     1321 cttcttccaa tcgatttcac tgtactcgaa ggaaacgcgc aaaagcatga ttttatagaa
     1381 gtaatgaagc agcaaattat aataatgcgt ccccataaac tgggataatt cattcttcaa
     1441 ttcgtctgca ggccgattgg atacgacaga gaagccatgc tcggtcgcaa tcataaacaa
     1501 atggggcgcc caacggtcgt gcaatgattc ggccaaggaa cgtcgaatat agtccggatt
     1561 cgtcgaagaa ataaaagggg ctccatcggg gctcctgcca ttcaaatttc cgatccggta
     1621 cagctgctcc tctgaaatcg ttgcgcctgt ttccgtaatt agaaaagaag aaacgaacat
     1681 cctctcatcc tcgaaatacg gaagcgatcc aaaatggcct ttcagtttct tgtcatgtaa
     1741 aacggtctct ttgataaagg ggcataaatg ctcgaataag taatcgtgaa tggagaattt
     1801 ttgtcctgat tcaggaatgt ggatcatagc ccctttactt tccttgagat tcggttcaat
     1861 tgcacggaag tgttgtatga aatccagcac atcgcataag tcagcggacg catcctccat
     1921 ttctatccgc aatgccagga aagcgatgcc gaaaggtcca aggatgatat cgatgctcct
     1981 tatgtaaaaa tcataccgtt catttcttaa ctgcatgata tggcctttga ggataggtgc
     2041 ggagtaacga tggaatcctt tcctttcatg cgaaccaggg aaaagtttat gttctatata
     2101 tggatagaag tattgttcga gttctttgcc gacaacttcg attccatggc catacatggc
     2161 ccgttcatac tttgcttcct catctatatg aaaataagtg tatccgttcg cttccaagct
     2221 atcgattgtt tgccgtcttt tcatctgatc aaagccgaat ggaaggatga acgtcatatg
     2281 agagcggatc atattcaatt tttctacttc catgggtatt ggcatagcat tgccttcctt
     2341 cctccgattc tcattgagtg gcaagccact tattaaacca ttccctttga caaagcacgg
     2401 ctaaacattc tatcccgatt tcaaattcat ccgcatagaa taagcagtgg gagtttttac
     2461 tcggaggaga taaagtatga aattacacga acagatgccg gaattgaatg gtgcgaccgt
     2521 ctggttgaat ggacgatatg aaaaagcgga ccttatcgga agaagtccga cgctcatcca
     2581 tttttggtca atcagctgcc gcctttgtaa ggaatcgatg ccctatatga ataacttgcg
     2641 aagtcgattt aaggacagat tgaatatcat tgcggtgcat atgccgcgat ccgaagaaga
     2701 tttaaacttg cagcagatta aaaaagttgc cgcggaacat aatatgacac acccaatttt
     2761 cgtggacggc gaattgatat taaccgacgc gtttgataac caatacgtcc ctgcctatta
     2821 tcttttcgat aaaaaggggc gactgcgcca ttatcaaaca gaacgaagcg gcatgggcat
     2881 gcttaggaaa cgtgtagacc gcttattgaa tgaaatggat agaggggacg gccgaattta
     2941 ggtcgtcttt tctttttcca gcgaaaataa tatgctacac taattttatc cataaatcgg
     3001 attggagcgg aattgaatga aaaaggaatt tgtcgtcatt ggattaggac gattcggggg
     3061 aagtatcgtc aatgaattgg tggaattaag tgtagatgta atggctatcg atatttcgcc
     3121 tgatcgtgta gaggaatttg cacagattgc aacgactgcg gtagctgccg atacgaccga
     3181 tgaatcggca ctccgatctt taggaatccg gaattttgag cacgtcgtcg tggcgatagg
     3241 ggaaaatatc caagcgagca ttttgacgac actcatcctg aaagaaatcg gcgtcaagag
     3301 aatcaccgtt aaagcgcaga atgactacca cgcgaaagtt ctacggaaaa tcggcgcgga
     3361 tcaagttgtc cacccggagc gagatatggg gaagaggatt gcgaacaatt tagtttccaa
     3421 taatattttg gattacctcg agctgtccga tgaatattcg attgcggaga tccgtgcgaa
     3481 tgagaagctt gcagggttca cgctgattga tcttgatatc cgtgcgaaat acggagtcaa
     3541 catcgtagcg attaagcgcg aagcgcaaat tttagtgtcg ccgcaagcca tcgaagaaat
     3601 ccaattgaat gacattttga ttgtaatcgg ttctgatttg gatattcata gatttgagaa
     3661 gaaggccctt cattgatgaa tggtcttttt ttgtgtaatt aaacggattt ccacttcagg
     3721 cggacgcttt ccaatcgaac agcaaagggt tcgatttgcc gtatttttgc gccttttgcg
     3781 gaaattaagg cagccctaca aaccctgctc ccttaaggtc gcaaaaaaca ttgctcgcaa
     3841 cgctacgctt gtgctcgcaa aagccgttct tcgtaacggc tttcgcagga gtcgccgccc
     3901 tccgtttcaa tcctagctaa atatattaaa aataaaaaag ccctcatatg agggcttatt
     3961 agtaagtcag acttccataa cgacaggcaa taccattggt ctacgttttg tctggtcgta
     4021 taaataaggt gaaaggatat cgataatgtc gctcttcaat gaacctgctt cagcagggga
     4081 gtttgcgagt ttcttgttca attccttcga taataaatgc tgcgcttcgc tgatcatcat
     4141 tccggattcc cgcatataaa caaatccgcg tgaaatgata tccggtccgg aaacaacttt
     4201 attgcgtttc ttatcaactg ttgcaacaac gatgaccaat ccgtcctccg acaggatttt
     4261 acggtcgcgc aagacgacgc ttccgatgtc accgatgcca cttccgtcga tgtaaacgtc
     4321 gccggatggg acttttcctg ctaatctggc cgtatcttcc gtcaaagcaa ggacatcacc
     4381 attgctcatg atgaaaatgt tgtccggatc gacgtcacag ccgatcgcaa gctctttatg
     4441 cattgtcaac atgcggtatt caccgtgaat cggcatgaag tatttcggtt tgatcaaccg
     4501 gagcatgagc ttctgttcct cctgagagcc gtgacccgat gtatggatgt tattcagtgc
     4561 gccatgaatg acttcggcac ccgcacggaa gagggcgttg atcgttttat tgacgctaag
     4621 tgtattccct gggatcggcg aggatgaaaa gatgaccgta tcgccaggtt ggatttgcac
     4681 ttggcggtgt gttccgtttg ctatcctcga taatgcagcc atcggttcgc cttggcttcc
     4741 cgtacagagg atcatcactt cgttcgcagg caatcggtta agcgactggg tatctacgaa
     4801 taactcttta ggtgcgttga tgtacccgag ttcccggccg attgtgattg cattgtccat
     4861 gcttcttccg aatacagcga ttttgcggcc gtggcgttca gcagcttcga tcacttgctg
     4921 taaacggtga atattggacg cgaatgtagc aaagatgatt cttccgtcga ctttccggaa
     4981 gatttcattc aagctatcgc cgactttcct ttccgacatc gtgaagttcg gcacttcagc
     5041 gttcgtactg tccgatagaa gacagagcac accgtcgctg ccgaatttcg ccatcttggc
     5101 gaggtctgca ggttcaccga caggcgtgaa atcaaatttg aagtcacctg tatggacgat
     5161 gttgccggaa ggcgttttga cgacgacacc gaatgcatca ggaatactat gggttgtcct
     5221 gaagaatgag acggaagtct tcctgaactt gaatacatca tcatccttga actcgatcat
     5281 cttggtggat ctgagaaggc cgtgttcatc aagcttattc cggagcaatc cgagcgccaa
     5341 tttactaccg tagaccggta tgttgatctt gcgtagtaaa taggggattc cgccgatgtg
     5401 gtcctcatga ccgtgcgtga tgaagaggcc tttgatttta tcgacattcc gttccaaata
     5461 ggtgtaatcc ggaatgacgt aatcgattcc gagtaaatcg tcctcgggga atttgatgcc
     5521 cgcatcaatc aggatgattt cgtcctggaa ttgcacacaa tatgtgtttt tgccgatttc
     5581 tccaagtccg cctagggcga aaacggctgt ttcgttattt tttattagtt tcataatgct
     5641 tagacctgct ctagctcgaa atgaggggat gcttgctcat attcgaggtg gttgccttcg
     5701 agcagctgga tcagttcaat attgtactcc cggttcttta gcttttcgcg gacagatcgt
     5761 tcagaatcgg cttcgatgta cagatgtttt gtgttttcac ggat
//
LOCUS       DASULI010000165         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1603653,
            whole genome shotgun sequence.
ACCESSION   DASULI010000165 DASULI010000000
VERSION     DASULI010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603653"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFX34_03580"
     CDS             complement(<1..749)
                     /locus_tag="VFX34_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX5564017.1"
                     /translation="MKPLTTAYIRKVISGKLIHGRDDLVIHHGAYRLKQVKHPHTLLF
                     LQRKIVRWDRLKPYYPIAIVTDRELAVSERNENLTIIKVENLQDSYWKFIDNYRASIN
                     IPIIAVTGTSGKTTTKEMIRHLLSISRNVAHTNSTNNSRTAHLTYLLGMDDTTEAAVF
                     ETAVGAPGDITNAAAYLKPTIGIITNIGEHHLNYCKTLEGYIAAKGEMCDALQPDGIL
                     IINADDRNTKKLELRNFTGKIIKVGIHSKSD"
     gene            929..2146
                     /locus_tag="VFX34_03585"
     CDS             929..2146
                     /locus_tag="VFX34_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5564018.1"
                     /translation="MKTIIFIGTNKSGSSREAIRAAERLGYFTVLFTHNDKQIKQRKE
                     YVDVHEMILVDTTNMTAMRKEILSLQSKGNEIMTIASFVDSNIARALKLCEEFCPNGT
                     SSKAADIMENKAGTRTSLNGLPFTPKFLTLPPKAKLESSTIGALPGFPLIVKSASSTG
                     SKDVLQATDLDQLERHIQKLMEKAPDESIIIEEFIAGEQYLVEAIIHEGAVQIAGVIQ
                     QEITYGKRFIITGYGVLAFVPKQLNDSIMEVLNAIIKRFDIQNGAMHVEMRLTETGWR
                     LIEINPRISGGAMNNMLKAAFGFDLVEETLKLYLGEKPSLVQKQRNFVFTQYVIIDQK
                     GTLEKVTGKKRARKMPGVVEVYVKPRKGTQLMPPLSMGHRYAYVIAQGRTLETAKALA
                     KKAAEEIQFHLKQ"
     gene            complement(2440..>2825)
                     /locus_tag="VFX34_03590"
     CDS             complement(2440..>2825)
                     /locus_tag="VFX34_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5564019.1"
                     /translation="DTVHSHPEGVRAAIDVLMNLAKHRKIAIIGQMRELGDIREREYR
                     KLGDYIQQQGIDLLITYGFRTEEIGAQAKRRGMAADKIHHFTDREKLHALLMNLISKD
                     DTILIKGASKTNMFETVKFIDQNYS"
BASE COUNT          772 a          645 c          635 g          773 t
ORIGIN      
        1 aaatcacttt tcgaatggat gccgacttta atgatcttgc cggtgaaatt tctcaactcc
       61 aacttctttg tgttccgatc atccgcgtta atgatgagaa tgccgtctgg ttgaagagcg
      121 tcgcacattt cccctttcgc agcaatatag ccttccaatg tcttgcagta attgagatga
      181 tgttcaccga tgttcgtaat aatccctatt gtcggcttca aataggccgc cgcatttgta
      241 atatcgccgg gcgcccccac cgccgtttca aacaccgcag cttctgtcgt gtcatccatt
      301 ccgagcagat acgtcaaatg tgcggtcctt gaattgtttg tactattcgt atgtgcgaca
      361 ttccgactga tcgataacag atgtcttatc atctccttcg tcgtcgtttt cccggatgta
      421 cctgttacgg cgattatagg gatattgatg gaagcccgat agttatcgat aaacttccaa
      481 taggagtctt gtaggttttc tactttgatg atcgtcaaat tttcattccg ttcgctgact
      541 gccaactcac gatctgtcac aattgctatc ggatagtaag gcttcagacg gtcccacctg
      601 acaattttcc gttgtaaaaa taatagtgta tgtggatgct tcacttgttt aagccggtag
      661 gcaccatgat ggataaccag atcgtctctt ccatgaatta atttgccgga tatgactttc
      721 cggatatatg ctgtcgttag aggcttcaat tggttcccct cccttccccc tttactatcg
      781 tatgccccga cacttcgaga agcctgtacg aaaaacctgt gtaaaccata tcggcgatag
      841 ttactgacct atatgatgta taaccatgtc gataaacgat tccttcataa cctatataga
      901 gttgtcttat atgaaggagt tgggcttgtt gaaaacgatc atctttatcg gaacaaacaa
      961 atcgggttca agcagggagg caatccgggc tgccgagcgg ctcggttact tcacggtgct
     1021 attcacacat aacgataaac agatcaagca aaggaaagaa tatgtggatg tccacgaaat
     1081 gatacttgtc gacacgacca atatgacggc gatgcggaaa gaaatccttt ctttgcaatc
     1141 caagggaaac gaaattatga cgattgcaag ttttgtcgat tccaatattg cccgtgcgtt
     1201 gaaactatgt gaggaattct gtccgaacgg cacctcttca aaagccgcgg atattatgga
     1261 gaacaaagca gggacaagga cttcattgaa tgggctccca ttcaccccaa agttcttgac
     1321 gcttccgcca aaagcgaagc tcgaatcttc aacaattggt gctttgcccg gttttccact
     1381 cattgtaaaa tcagcttcct ctacgggatc aaaagatgtt ctacaagcaa ctgatttgga
     1441 ccagttggag cggcacatcc aaaaattgat ggaaaaggca ccggatgagt ccattattat
     1501 cgaagaattc atcgcagggg agcaatactt agtcgaagcg attatccatg aaggagcagt
     1561 tcaaatcgcc ggcgtcatcc aacaggaaat cacatacggc aaacgcttca tcatcactgg
     1621 ttacggcgtg ctcgcatttg tgccgaaaca attgaatgac agcatcatgg aagtgctgaa
     1681 cgccatcatc aagagattcg atatccaaaa cggagcgatg cacgtcgaaa tgcgtctgac
     1741 ggaaaccgga tggcgcctca tcgaaatcaa tcccaggatt tcaggcggag caatgaataa
     1801 tatgctgaaa gctgctttcg gttttgactt ggtagaagaa acactgaaat tatatttagg
     1861 tgaaaaacct tctttggtac agaaacaacg gaatttcgtc ttcactcagt atgtcatcat
     1921 cgatcaaaaa gggactttgg aaaaagtgac cggtaaaaaa agggcaagaa aaatgcccgg
     1981 agtcgtagaa gtatatgtca agccacggaa agggacgcaa ctgatgccac ccctgtccat
     2041 gggtcatcgg tatgcctatg tgatcgcaca aggcaggacg cttgagactg caaaagcatt
     2101 ggcgaaaaag gccgctgaag aaatccaatt tcatctgaag caataaaaga gaggactccc
     2161 attaagcgga gtcctcattt ttaagcaaaa gcgtaggtcg cctgcctaga tgcgacaggc
     2221 aaagccgtaa cgtagaacgg cttttgcgag tataagcgaa gcgttaggag caatagtttg
     2281 tgcgagtata agcgaagcgt taggagcaat ggtttttgcc atacagcatc tttgacttat
     2341 gaccccgagc aaatcgaact gcgaatggtt cgatttgctg tatttctgcg gtttttgcaa
     2401 aaattaaggc atccttcctg cgaccgaagc tggatgattt caactatagt tttgatcgat
     2461 gaatttaaca gtttcgaaca tattcgtctt actggctcct ttaataagga tcgtgtcatc
     2521 tttactgatc aagttcatca gcaatgcgtg caatttctcc cgatctgtga aatggtggat
     2581 tttgtccgca gccatgcctc tcctcttcgc ttgggcgccg atttcctccg tccggaatcc
     2641 gtaggtgatt aacagatcga tcccttgctg ctgaatatag tctccaagtt tcctgtactc
     2701 cctctcacga atatcgccaa gttccctcat ttgtccgatg atggcgattt tacgatgctt
     2761 tgctaggttc ataagtacat cgattgctgc ccggacccct tccggatggg agtggaccgt
     2821 atcgt
//
LOCUS       DASULI010000166         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1519208,
            whole genome shotgun sequence.
ACCESSION   DASULI010000166 DASULI010000000
VERSION     DASULI010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519208"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFX34_03595"
     CDS             <1..323
                     /locus_tag="VFX34_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5564020.1"
                     /translation="NSVKDGLTATRHSLDTFTEIEHSVNAVNENASIVTEAIKQIRSM
                     TDTIALSSREVKKLAESTALTAQETSAATEEQLAVNEEISTSSQTLAGLAENLQKEVN
                     RFTF"
     gene            complement(387..674)
                     /locus_tag="VFX34_03600"
     CDS             complement(387..674)
                     /locus_tag="VFX34_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006037117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6509 family protein"
                     /protein_id="HEX5564021.1"
                     /translation="MEIIEYSMIELIDPTGILTGNRYEFRIYVNFDEEDELYNEKGTG
                     VRVIYAVDEGEGKVASYHLFDRATDAVLDFDLEDEEVAELATFCSEQLEEE"
     gene            859..>2541
                     /locus_tag="VFX34_03605"
     CDS             859..>2541
                     /locus_tag="VFX34_03605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5564022.1"
                     /translation="MLTFEEFEYKRPDMDSIKQEFNVLLEQFRTAESFKEQNGVIEKL
                     NAIGSNYSTQSNLMYIRSSIDTNDEFYQKEREYFDEIGPEFQELGTAFYKELVKTPYR
                     KELEEKWGTQLFALADNAIKSFSPEVLPLLQQENKLTSEYAKLVASAQIEFDGKTLTL
                     AQLGPYAESTDRGVRKSAMEASYSFYADNGEAFDRIYDDLVKVRHEIATKLGFKNFVE
                     LGYARMNRIGYDADMVKNFRDQVRDYIVPLATKLYERQAERIGVDELKFYDQSLNFLS
                     GNATPKGSPEWIIENGKKMYAELSPETEEFFNYMTDKHLLDLEAKKGKETGGYCTFID
                     DYDSPFIFSNFNGTSGDIDVLTHEAGHAFQVYSSRNIGIPEYVWPTHEGAEIHSMSME
                     FFTWPWMEMFFEDDTEKYKFAHLSSGLLFLPYGVAVDEFQHAVYEKPEMTPAERKAEW
                     KRIEKTYLPMRDYDGNPYLEAGSIWQRQSHIYEVPFYYIDYTLAQICAFQFWKRSFED
                     REAAWKDYLHLCRLGGSKPFTELVSEANLISPFDDGCVESVVGTIEIWLNSVD"
BASE COUNT          744 a          556 c          585 g          656 t
ORIGIN      
        1 gcaacagtgt gaaggacggc ttgacagcca cacgccattc attggataca tttactgaaa
       61 tcgagcattc cgtcaatgca gtgaatgaga atgcatcgat tgttaccgaa gcgattaagc
      121 aaatcagatc gatgactgac acgattgccc tttcatcgag ggaagtgaaa aaactggctg
      181 aatcgactgc attgacagcg caggaaacga gcgcagcgac cgaagagcaa ttggccgtca
      241 atgaagaaat atctacaagc tcgcaaacgc ttgcaggact agccgagaac cttcaaaaag
      301 aagtgaaccg ctttacattt taaactaaaa atcgttgaaa ctggattccc atacagccgg
      361 aaccgtttca acgatttttt cttcgcttac tcttcttcta attgttctga acaaaaagtt
      421 gccagttcgg ccacttcctc atcctctaag tcgaaatcga ggactgcgtc tgttgcacgg
      481 tcgaaaagat gataggacgc cactttccct tcgccttcat caacagcgta gatgacccgg
      541 acgcctgtcc ccttctcgtt atacaattcg tcttcctcat cgaaattaac gtatatgcga
      601 aattcgtacc ggttacctgt caagattcct gttgggtcaa taagctcgat catactatat
      661 tcaataattt ccacgtagtc tcatcctcac aaattatttt tcatactcat cttacagcaa
      721 acggaatcca tggtcacgtt ttcacttcca aaaaaattct gttccggctc gtaatctttc
      781 ttttgcattt tcacaatgat tagttaacaa tgaagatagt gaattgtatt cacactaaga
      841 gattacgggg gaatgaaaat gcttacattt gaagaatttg agtacaaaag acccgacatg
      901 gacagcataa aacaagaatt caatgttctg ctggaacaat tccgcaccgc ggagtcgttt
      961 aaagaacaaa atggcgtcat tgaaaaactg aatgccatcg gaagcaatta ttccacacaa
     1021 tccaacttga tgtacatccg ttcatccatc gatacgaatg atgaattcta tcagaaggaa
     1081 cgggaatatt tcgatgagat cggaccggaa ttccaggagc tagggaccgc tttctacaag
     1141 gaattggtga agacccctta tcgcaaggaa ctggaagaga aatggggaac gcagctgttc
     1201 gcccttgcgg acaatgcaat caaatccttt tctccggaag tccttccgct tctacagcaa
     1261 gagaataagc ttacctccga atatgcaaaa ctcgtagcat ccgctcaaat cgaatttgac
     1321 ggaaaaacat taacgctcgc acagctcgga ccttatgccg agtcgactga cagaggcgtc
     1381 aggaagtcag caatggaagc ttcctattcg ttctatgcgg ataatggaga ggcttttgac
     1441 cgcatctatg atgaccttgt caaagtgaga catgaaatcg ctacgaaatt agggttcaag
     1501 aattttgtgg aactcggata tgcgcggatg aaccggattg gatacgatgc ggatatggtg
     1561 aaaaatttcc gtgaccaagt acgggactat attgtaccgc ttgcgacgaa actatacgaa
     1621 cgccaagcag aacgcatcgg tgtcgatgaa ttgaaattct atgaccagtc ccttaacttc
     1681 ctaagcggga atgcgacccc gaaaggttcg cctgagtgga tcattgaaaa cggtaaaaag
     1741 atgtatgcgg agctttcacc tgaaacggaa gaattcttca actacatgac agacaagcat
     1801 cttcttgatt tggaagcaaa gaaagggaaa gagacgggtg ggtattgcac gttcatcgat
     1861 gattatgatt cgcctttcat cttttcgaac ttcaatggta catcaggcga catcgatgtg
     1921 ctgacacacg aggcgggcca tgctttccaa gtatactcca gccgtaatat cggcattccc
     1981 gaatacgttt ggccgacaca tgaaggagcg gaaatccatt caatgagcat ggaattcttc
     2041 acttggcctt ggatggaaat gttctttgaa gacgatacgg aaaagtataa gttcgcgcat
     2101 ttgagcagcg gcttgctctt tttgccgtat ggtgtagcag ttgatgaatt ccagcatgcg
     2161 gtttatgaaa aaccggaaat gacgccagct gagcgcaagg cggaatggaa gcggattgaa
     2221 aagacatact tgccgatgcg tgattacgat ggcaatcctt atttggaggc aggttcaatc
     2281 tggcaacgcc aatcgcatat ttatgaagtc cccttctact atatcgacta tacattagcc
     2341 caaatttgcg cattccaatt ttggaagcgc tccttcgagg atcgcgaggc tgcatggaag
     2401 gattatttgc atctatgcag gcttggcggg tcgaagccgt ttacggagtt ggtaagcgag
     2461 gcgaatttga tttcgccgtt cgacgatggt tgtgttgagt cggttgtcgg gacgattgaa
     2521 atatggttga attcagtcga c
//
LOCUS       DASULI010000167         5953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1162024,
            whole genome shotgun sequence.
ACCESSION   DASULI010000167 DASULI010000000
VERSION     DASULI010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162024"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1339
                     /locus_tag="VFX34_03610"
     CDS             146..1339
                     /locus_tag="VFX34_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEX5564023.1"
                     /translation="MSVSEKIISQTNKFGANNYHPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPRIIQALKDQADKVTLTSRAFHNDQLGPWYELICKLSGKDMTLP
                     MNTGAEAVETAIKAARRWAYDVKGVEENKAEIIGCNGNFHGRTMTAVSLSSEAEYKRG
                     FGPMLPGIKLVDFGDLKALEEAITPNTAAFIIEPIQGEAGILIPPAGYMKAAYELCKQ
                     HNVLFIADEIQAGLCRTGKMFACEWEGFEPDMYILGKALGGGVFPISCVVANKDILGV
                     FNPGSHGSTFGGNPMACAVSIASLEVLQDEKLADKSLELGNYFIEQLKKISHPSIKEV
                     RGRGLFIGVELTEAARPYCEKLKELGLLCKETHDTVIRFAPPLIITKEELDWALDKIN
                     KVFTN"
     gene            1426..2670
                     /locus_tag="VFX34_03615"
     CDS             1426..2670
                     /locus_tag="VFX34_03615"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5564024.1"
                     /translation="MTENLNLFTSTQVVIKDALDKLGYDEGMYDLLKEPIRMVEVRIP
                     VRMDDGKVKVFTGFRGQHNDAVGPTKGGVRFHPGVTADEVRALSMWMTLKAGIVDLPY
                     GGGKGGIICDPREMSMGELERLSRGYVRALSQVMGPTKDIPAPDVFTNAQIMAWMMDE
                     YSRIDEFNSPGFITGKPIVLGGSQGRDRATAEGVTIIINEAAKKRGIDMKGARVVIQG
                     FGNAGSFLSKFLHDAGAKVIGISDAYGALHDPDGLDIDYLLDRRDSFGTVTTLFDNTI
                     TNAELLELDCDILVPAAIENQITEKNANNIKATIVVEAANGPTTSEATKILTDRGILL
                     VPDVLASAGGVTVSYFEWVQNNMGYYWTEEEVREKMTTKMVDAFENVYNVASTRNIDM
                     RLAAYMIGIRKTAEASRFRGWA"
     gene            complement(2839..3075)
                     /locus_tag="VFX34_03620"
     CDS             complement(2839..3075)
                     /locus_tag="VFX34_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX5564025.1"
                     /translation="MVQKLALAITIIGALNWGVVGLFSFDVVAQFADGHAQPFARFFY
                     IVVGISGLISLGLLFDTVREKQVEHVTVPAPEEV"
     gene            3180..4346
                     /locus_tag="VFX34_03625"
     CDS             3180..4346
                     /locus_tag="VFX34_03625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019715531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX5564026.1"
                     /translation="MKEFVFQNPVKLIFGKNSIAKLATELSAYGDRILVVYGGGSIKR
                     NGLYDEVMAILKEEKKEVFELAGVEPNPRVSTARKGAALCKEHNIDFILAVGGGSVID
                     CTKLIATAAKYDGDPWDFVIKKDSPKDAIPFGTILTLAATGSEMNAGSVITNEETEEK
                     YGWGGPFNYPKFSILDPTYTLSLPKDQTVYGIVDMMSHIFEQYFHSATNTPLQDELCE
                     GALRAIMEAGEKLVDDLQNYELRETILLGGTWGLNGFLGMGNNGGDWGTHDIEHAVSA
                     VYDIPHAGGLAILFPHWMRHTLKLNPARYARLAVKVFGVDPEGKTDEAVAHEGIDRLR
                     AYWTAIGAPEKLSYYGIDDSKVDLMAKKAAINGPLGRYAQLSEEEVKSILEASL"
     gene            complement(4372..5394)
                     /locus_tag="VFX34_03630"
     CDS             complement(4372..5394)
                     /locus_tag="VFX34_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5564027.1"
                     /translation="MKDILRLLKDGNKPSLLAGIVNAAIAILKAGAYMLTGNVAMFAE
                     TLHSLGDAANQFFVYIGSALSKKAPTPNFPNGFGRVVNLVCLGAVIIVGIMSYEAVKE
                     GWHHVLNPTETKGLLINLSVLGLATALEFFVLLKASKEVAHESGQAHSGLKAFAYSLT
                     HLDKAKPATKLVFMEDMVATIGGLLAFVAVLIAHFTGFLRAEGIASMMIGIMMFYVVG
                     RVFLENARGAIGETDEEMLNHIAHIIAEDPDIKDIQKVEVIKEGEYLHVEVVAEVDPS
                     QTVAYIDDVRDRLMALVLKQKGVREALISFDEDDGVKTWQGVKETTMTEEARRKLPEN
                     NKQRAE"
     gene            complement(5410..>5953)
                     /locus_tag="VFX34_03635"
     CDS             complement(5410..>5953)
                     /locus_tag="VFX34_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5564028.1"
                     /translation="AYLVPARDDINVTMDPLRLAAQIVSGVGFLGAGAILRRENDNIT
                     GLTTAAMIWGAASIGIAVGAGFYIEASFAVLSILFVIEVIAPALGKFGPKRIRSKEAA
                     CIVVVSDKSKIDDLIGILNDENMKVENLRIRQVHFDDKPSVHEVDFRLDALPKKGTTQ
                     LYTELTELPYIKSVEIEIFP"
BASE COUNT         1687 a         1317 c         1455 g         1494 t
ORIGIN      
        1 ccggaaccga ctccaaagcg ggcggcccag actacttgca gttacacatg caaggaaaaa
       61 cgacttctga gactctttaa ttaaatgaag gaagcagttc tggtgactgc ttcctgctat
      121 acatacaaat ggggagggga aatacatgtc ggtttctgaa aagatcattt cacaaacgaa
      181 taaattcggg gcgaacaact accatccact gccaatcgtt atttctgaag cggaaggtgt
      241 ttgggtcaag gatcctgagg gaaataaata catggatatg ctatccgcat attccgcagt
      301 gaaccaagga catcgccacc caagaatcat ccaggcattg aaggatcagg cggataaagt
      361 gacattgaca tcacgcgcat tccataacga tcaacttggt ccatggtatg aattgatttg
      421 caaactatcg ggcaaagaca tgacattgcc gatgaataca ggggcagaag ctgtggagac
      481 ggctatcaaa gcagctcgtc gctgggctta tgatgtcaaa ggcgttgaag aaaataaagc
      541 tgagatcatc ggttgcaacg ggaatttcca cgggcggacg atgacagcgg tctccctttc
      601 atcagaagct gaatataaac gtggattcgg tccgatgctt ccaggcatca agttggtaga
      661 cttcggtgat ctaaaagcat tggaagaagc aatcactccg aacaccgctg cattcatcat
      721 cgagccgatc caaggagaag cgggtatcct cattcctcct gcgggctaca tgaaagcagc
      781 ctacgaactt tgtaaacaac ataacgtcct atttattgca gatgaaatcc aggcagggct
      841 ttgccgtacg gggaaaatgt ttgcttgtga gtgggaagga ttcgaacctg acatgtacat
      901 tctaggaaaa gcgttgggtg gcggcgtatt cccgatttct tgcgtggtag cgaataaaga
      961 tattcttgga gtgttcaatc caggttcaca cggatctact ttcggtggaa atccgatggc
     1021 ttgtgctgtt tcaatcgctt ctttggaagt tttgcaggat gagaagcttg cggacaaatc
     1081 acttgaactc ggaaattatt tcattgaaca attaaagaaa atcagccatc cgtcgattaa
     1141 agaagtgcgc ggacgcggtt tgttcatcgg cgtcgaactt acggaagcag cacgtcctta
     1201 ttgtgaaaag ctgaaggaac tcggattgct atgtaaagag acacatgata ccgtcatccg
     1261 ttttgcaccg cctttaatca ttacaaaaga agaactcgat tgggcactgg ataaaatcaa
     1321 taaagttttt acgaactaaa caaaaacttc acaaaatcta ttaggtgtat gatacaatat
     1381 gtgcggtaga aaaaacatta tgaaatcaaa gaggcgaact tttacatgac tgaaaacctg
     1441 aatctgttta cgtctacaca agttgtcatt aaggatgcac ttgacaaact aggctacgat
     1501 gaaggaatgt acgatcttct gaaagaacct atccgtatgg tggaagtaag gatccctgta
     1561 cgcatggatg atggaaaagt gaaggtattc actggtttcc gcggacagca taacgatgca
     1621 gtcggtccga caaaaggcgg cgtccgtttc catccgggag ttactgcgga tgaagtacgc
     1681 gcgttgtcaa tgtggatgac actgaaagct ggaattgtcg acttgccata tggcggcggt
     1741 aaaggcggaa tcatctgtga cccaagagaa atgtcgatgg gtgaattaga gcgtttaagc
     1801 cgtgggtatg tacgtgcgtt aagtcaggta atgggaccga caaaggatat cccggcaccg
     1861 gacgttttca caaatgctca aatcatggct tggatgatgg atgaatatag tagaatcgat
     1921 gaattcaact ctcctggttt cattacaggc aaaccgattg ttcttggcgg atcccaagga
     1981 cgtgaccgtg caactgctga aggtgtaaca atcattatca atgaagcggc gaaaaaacgt
     2041 ggaatcgata tgaaaggtgc acgtgttgtt attcaaggat tcggtaacgc aggaagtttc
     2101 ctttccaaat tccttcatga cgcaggagca aaagttatcg gtatttccga tgcttacggc
     2161 gcgcttcatg atccggacgg cttggacatc gactatctat tggatcgtcg tgacagcttt
     2221 ggaacagtta caacactttt cgataacacg atcacgaacg ctgaactatt ggaattggat
     2281 tgcgatattc tcgttccggc tgctatcgaa aatcaaatca ccgaaaagaa tgctaacaat
     2341 attaaggcga cgatcgttgt ggaagcggca aatggtccaa caacttcaga agccactaaa
     2401 atacttacag accggggaat cctcctcgtg cctgacgtcc ttgcaagtgc aggcggcgta
     2461 acagtttcct acttcgaatg ggttcagaac aatatgggtt actactggac agaagaagaa
     2521 gttcgcgaaa aaatgacgac taaaatggtt gatgcgttcg aaaacgtata taatgttgca
     2581 tcaactagaa atatcgacat gcgtctagct gcatatatga tcggtatccg caaaacggcg
     2641 gaagcatcaa gattccgtgg ttgggcataa acaaaagcgt agagcgcctg cccagaggcg
     2701 aaaggcataa ggcgaagatg cggcgtggcg gtttttgcca cagagcagat ttgacttatg
     2761 accccgagct tctggcgctc ggagctagat gataattaag acaagaaagc atctcctggt
     2821 aaaacgggag atgctttttt acacttcctc cggcgccggg acggtgacgt gctccacctg
     2881 tttttcccgc accgtatcaa acagcaatcc gagcgagata agtcctgaaa tccctacgac
     2941 aatataaaag aagcgtgcga acggttgggc atgcccatcg gcgaattggg ctacgacatc
     3001 gaagctgaac agcccgacga ctccccaatt caaggcgcct atgattgtaa tggccaacgc
     3061 taatttttga accaaaaatg tcacctcctc aggttaggat tcccaatcca aacctttcca
     3121 tacttgcatc gctcaattca ttcttggata atgaacagta gaaataggag gtcatctaaa
     3181 tgaaggaatt tgtttttcaa aaccctgtta aattgatttt tggaaagaac tccatcgcca
     3241 agcttgcaac agaattatcg gcttatgggg accgaatcct cgttgtctat ggcggaggca
     3301 gcattaaaag gaacggcctt tatgatgaag tgatggcaat cctgaaggaa gaaaaaaagg
     3361 aagtgttcga gcttgccggc gttgagccga atccacgggt cagcacagct agaaaaggtg
     3421 ctgccctatg taaagagcat aatatcgatt tcattttggc ggtcggcgga ggatctgtca
     3481 ttgactgtac gaaattgatt gcgacagctg caaagtatga cggggatcca tgggatttcg
     3541 tcattaaaaa agactcgccg aaagacgcaa ttcctttcgg tacgattttg acgctagcag
     3601 ctacaggttc cgaaatgaac gcaggctccg tcattacaaa tgaagaaacg gaagaaaaat
     3661 acggatgggg cggtcccttc aactatccga aattctccat tctcgacccg acatacacgc
     3721 tctccttgcc gaaggaccaa actgtttatg gtattgtcga catgatgtcg catattttcg
     3781 agcagtattt ccatagtgcg acgaatacgc cgcttcaaga tgagttatgc gaaggggcct
     3841 tgcgagctat tatggaagct ggtgaaaaac tcgttgatga tctgcagaac tatgagttgc
     3901 gtgaaaccat cctcttaggg gggacatggg gattgaacgg atttttaggc atggggaata
     3961 atgggggtga ctgggggacg catgacatcg aacatgccgt atctgcagtt tacgacattc
     4021 cgcatgcggg aggattggcg attcttttcc cacactggat gaggcatacg ttaaagttga
     4081 acccggcgcg ttatgcgagg ttggcggtca aagttttcgg agtggatcct gagggtaaaa
     4141 cggatgaggc cgttgcgcat gaagggattg acagactgag ggcgtactgg acagcaatcg
     4201 gtgcaccgga aaagctgtcg tattacggca tcgatgactc gaaggttgac ctgatggcga
     4261 agaaggcggc aattaacgga ccgctcggga gatacgctca attgagtgaa gaagaagtta
     4321 aatccatatt ggaagcttcg ttataacaaa ggaaagcaga gggggaattg ttcattccgc
     4381 cctctgctta ttattttccg gaagtttcct tcgtgcctct tccgtcattg ttgtttcttt
     4441 caccccttgc caagttttga cgccgtcgtc ttcatcgaaa gaaatcaatg cttcccggac
     4501 gcctttctgc ttcaatacca aagccatgag cctgtccctg acatcgtcga tatacgcgac
     4561 agtttgtgac ggatccacct cagcaaccac ttcgacatga aggtattcac cttctttgat
     4621 cacttcgact ttttggatgt ccttgatatc gggatcttct gctatgatgt gcgcaatatg
     4681 attcaacatt tcttcatcgg tttcaccgat tgcacctctt gcgttctcaa ggaacactct
     4741 gccgacgaca taaaacatca tgatgccgat catcatggaa gcaatccctt ccgctcggag
     4801 gaatccggtg aaatgggcaa tcaaaacagc gacgaaagcc agtaagcctc ctattgtcgc
     4861 gaccatgtct tccatgaaga ccaatttggt agcgggcttg gctttatcca gatgggtcaa
     4921 gctgtaggcg aatgctttca accctgaatg cgcttgtcca gactcatgcg caacttcttt
     4981 tgaagccttc agtaacacga aaaactccag cgccgtggca agcccaagga cacttaaatt
     5041 gatgaggaga ccttttgttt cggttggatt caaaacgtga tgccaacctt ccttcaccgc
     5101 ctcgtatgac atgatgccga cgataatgac tgctcctaaa caaacgagat tgacaaccct
     5161 gccgaatccg ttcggaaaat taggggttgg cgccttcttg gacaatgcgg agcctatata
     5221 tacgaaaaac tggttagctg cgtctcctaa agagtgcagc gtttccgcaa acatggccac
     5281 gtttccagtc agcatatagg cgcctgcctt taggatggca atggcggcat tgactatgcc
     5341 ggcaagcaaa gacggcttat tgccatcctt caacagacgt aaaatatctt tcatatacat
     5401 gagcctccat taaggaaaga tttctatttc gaccgatttg atataaggga gttctgtcag
     5461 ttcggtatat aattgtgtag ttcccttttt agggagtgcg tcaagacgaa aatcgacttc
     5521 atgcactgaa ggtttatcgt caaagtgaac ttggcgtata cgcaagtttt ccactttcat
     5581 attttcgtca ttcaaaattc caatcaagtc atcgattttg cttttatccg aaacgacgac
     5641 aatgcatgca gcctcctttg agcggattct tttcggaccg aatttgccaa gggcaggcgc
     5701 aatgacttca atgacgaaga ggatgcttaa cacagcgaac gatgcctcga tataaaatcc
     5761 ggcgccaact gctattccga tactcgctgc tccccaaatc attgccgctg tcgttaaacc
     5821 ggtaatatta tcgttttccc ttcgtagaat ggcacctgca cctaagaagc cgactccact
     5881 gacgatctgc gcggcaagac gcaatggatc catcgtcacg ttaatatcgt ccctggcagg
     5941 tacaagatat gca
//
LOCUS       DASULI010000168         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_107062, whole
            genome shotgun sequence.
ACCESSION   DASULI010000168 DASULI010000000
VERSION     DASULI010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107062"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..648
                     /locus_tag="VFX34_03640"
     CDS             169..648
                     /locus_tag="VFX34_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5564029.1"
                     /translation="MRAQPANRISKKGLKVWRLYGWIQTIILALAATGGGVLVYIFEW
                     PWWIYPIEAIVLVLFAYFIIFLFPKVRWLRWRYEVRESEIELQHGLFIVKRTLIPMVR
                     VQHVDTSQGPILRKYGLAEISISTAATVHTIPALIMSEADELRGRISVLARVAEDDV"
     gene            638..>2101
                     /locus_tag="VFX34_03645"
     CDS             638..>2101
                     /locus_tag="VFX34_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5564030.1"
                     /translation="MMSEQQRYKLHWVTAIIEILKTLKEALLPLVVLIFANGWNQGRT
                     GLWFIDYASFIIFGVLIIFLAVAGIIKWKRFEYWFEDDELRIESGLFVKKKRYIPFDR
                     IQSLDYTEGIFHRPFGLVKVKVETAGSSNAKQAEGELTAITKEAAKRVEIEMAEAKKR
                     QREMPATEGGAAELLEEIPVAAEKKFRNIFTMTTKDLLVLATTSGGVGVILSGVAIFL
                     SQFGELLPFEWMFEEISAFIKFSVLIVAIAVFLGLLGVWILSVGMTLLSHFNFTVEMN
                     EEDIIITRGLLEKKKVTVPLKRIQSVVVVENPFRKLFGYATVAIHSAGGGIGQSSKIN
                     LFPIVKRGKVESYLEDIFPDLDLEEPTKKLTAKGRPFYYRIDFLWMVPVIGALTYFFF
                     PYGLFSLAIIPVIIAYGVWQHRSAAYDIAGNQLTMRFRTFSLQTAYMMKKRIQSMEMK
                     QSYFHKRKGVATVIANIKSGMGVYHATVQHTDIEEAEK"
BASE COUNT          533 a          444 c          579 g          545 t
ORIGIN      
        1 tatttctgtg tattttgcag aaattaaggc actatcagga cgcggtgcac ttaaactgcg
       61 aacctctact aacagccatc cttcgctttt gatttttatt tgcgtccaac gaaacatttt
      121 catcaatgca acgtatacaa tggaagaata gaaagaggtg ttgtggaaat gagggctcaa
      181 ccggcaaaca ggatttctaa gaaaggtttg aaggtatggc gtttatacgg ctggatccaa
      241 acgattattc tcgcattggc agccaccggc ggcggtgtgc ttgtatacat attcgaatgg
      301 ccgtggtgga tttatccgat tgaagcgatt gttcttgtgc tatttgccta tttcatcatc
      361 tttttattcc cgaaggtgag atggctcaga tggcgctatg aagtgaggga atccgaaatc
      421 gaattgcagc acggcctctt cattgtgaag cggacgctga ttccgatggt tcgtgtccag
      481 catgtcgata cttcccaggg accgatcctg cggaaatacg ggttggctga aatctcaatt
      541 tcaacggccg ctacggttca tacaatccct gcactgatca tgtcggaggc ggatgaactg
      601 cgaggcagga tttccgtgct tgcaagggtg gcggaagatg atgtctgaac agcaacgtta
      661 taaattgcat tgggtcactg ccatcattga aatactaaaa acgttgaaag aggctcttct
      721 tccgcttgtc gtgttgatat ttgcgaacgg atggaatcag ggaaggactg gactctggtt
      781 tatcgattac gcgtctttta tcattttcgg tgtcctgatc atcttcttgg ccgttgcggg
      841 aatcatcaag tggaaacgtt ttgagtattg gttcgaggat gatgagcttc ggatcgaatc
      901 gggtttattc gtgaagaaga aacggtatat ccctttcgac cggatccaaa gcctggatta
      961 cacggaaggg attttccacc gtccgttcgg gcttgtgaaa gtgaaagtgg agacggcggg
     1021 cagttctaac gcaaagcaag cggaagggga attgacggcg attacgaagg aagccgccaa
     1081 acgcgtcgag atcgagatgg ccgaggcgaa gaaacgccaa agggaaatgc cggccacaga
     1141 agggggggcg gcagaacttc ttgaagaaat acctgtggca gcagaaaaga agttccgcaa
     1201 catcttcacg atgacgacga aggatctgtt ggtgcttgcg acaacttccg gcggtgtagg
     1261 ggtcatcctg tccggagtgg cgatcttcct atcacagttc ggtgaattgc ttcctttcga
     1321 gtggatgttt gaggagatat cagcattcat caagttcagt gtactgatcg tagcgattgc
     1381 cgtattcctc ggcttgctcg gtgtatggat attatccgtc ggaatgacgc tcctgtccca
     1441 tttcaatttc acggttgaaa tgaatgaaga agacattatc attacgagag gattgcttga
     1501 gaagaagaaa gtgaccgtac ctttgaaaag gatccaaagt gtcgtagtcg tcgagaatcc
     1561 gttccggaag cttttcggct atgctactgt agcgatccat agtgcaggtg gcggcatcgg
     1621 ccaaagctca aagatcaatc tattcccgat tgtgaagagg ggcaaggtgg agtcgtattt
     1681 agaggacatc ttcccggatc tggatttgga ggagccgacg aaaaagctta cggcaaaagg
     1741 caggccgttt tactatcgca tcgactttct gtggatggtg ccggtcatcg gcgcgctcac
     1801 atacttcttt ttcccgtatg gcctgttttc gctcgccatc atccctgtca tcatcgcgta
     1861 tggcgtgtgg cagcatcgtt ccgccgcgta tgatattgcg gggaatcaat tgacgatgcg
     1921 gttcaggaca ttcagtttgc agacggcgta tatgatgaaa aagcggatcc agtcaatgga
     1981 aatgaaacag agctatttcc ataaacgaaa aggggttgcg acggtcatcg ccaatatcaa
     2041 atccggcatg ggcgtctatc acgccacggt gcagcatacg gacatcgaag aggcggaaaa
     2101 g
//
LOCUS       DASULI010000169         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_370808, whole
            genome shotgun sequence.
ACCESSION   DASULI010000169 DASULI010000000
VERSION     DASULI010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370808"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFX34_03650"
     CDS             complement(<1..304)
                     /locus_tag="VFX34_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5564031.1"
                     /translation="MFRDISLQDLFLLKDKQPHTIIDVRSPAEYKEATIPGCINIPVF
                     SDEERAEIGTLYKQVSKEAATERGLEIFSAKLPAFIQHFKTIDTPITVFCWRGGMRS"
     gene            complement(328..1377)
                     /gene="selD"
                     /locus_tag="VFX34_03655"
     CDS             complement(328..1377)
                     /gene="selD"
                     /locus_tag="VFX34_03655"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX5564032.1"
                     /translation="MKVPNAVKLTSLTKKGGCGCKIGPADLSQVLQSLPPAVFDPNLL
                     VSLDTSDDAGVYKLTDEIAIVQTLDFFTPIVDDPYDFGQIAATNAISDVYAMGGKPIT
                     ALNIVAFPISTLDKSILSDILRGAGDKLKEAGVTLVGGHSIDDQEPKFGLAVTGTVHP
                     DKIRTNAGAKTGDRLVLTKPIGVGIYTTSLKRSVLTSEEIKQVTSIMTTLDKTASEVM
                     ASFEVHAATDVTGFGLLGHATEMAKASDKGIRIYAKQVPVLPRTKELAEAGMIPGGTK
                     SNYAHVADVVTYSDELDMLDRYILCDAVTSGGLLLAVSANEAEELVDEMTKKGIKAAI
                     IGEVTDNHPGMITVD"
     gene            1544..>2150
                     /locus_tag="VFX34_03660"
     CDS             1544..>2150
                     /locus_tag="VFX34_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5564033.1"
                     /translation="MNTFSMVIDGKEVGKDLESIDVINPATGESIATVPDGGRKEAVL
                     AVDAAYEAFQKWSKLTTYERSSLLNKWHALIDLHMDELARTMTEEQGKPLAEAVGEMK
                     YANGFISWYAEEAKRIYGEMIPSSHTGKRIFIHKQPVGVTAVITPWNFPAAMITRKVG
                     PALAAGCTVVIKPAEQTPITAIRLAQLALDAGIPAGVINVVT"
BASE COUNT          585 a          477 c          490 g          598 t
ORIGIN      
        1 tgcttcgcat tccgccacgc cagcaaaaga ctgtaattgg agtatctatc gttttaaagt
       61 gttgaatgaa ggcgggaagt tttgctgaaa atatttccag tcctcgttca gtcgccgctt
      121 ctttgctgac ctgtttataa agagtaccaa tctcggcccg ttcttcgtcc gaaaatacag
      181 gaatgttaat acatccaggg attgtcgcct ccttatattc ggcgggtgac ctgacatcga
      241 taatcgtatg tggttgttta tcttttaaaa gaaaaaggtc ttgtaaacta atatcccgaa
      301 acatcttttc accttcaata gtaaaggtca atcaacggta atcattccgg gatgattgtc
      361 tgtaacttcc ccgatgattg ccgctttgat gcccttttta gtcatttcat ccactagctc
      421 ttcagcttca tttgcagata ctgctagtag cagaccgcct gacgtcactg catcacacaa
      481 aatataacga tccaacatat caagttcatc ggaatatgtg accacatcag ccacatgggc
      541 atagttgctt ttcgttcctc caggaatcat acctgcttct gccagctcct ttgtccgggg
      601 aaggacaggt acttgcttcg cataaatccg gatgccctta tcacttgcct ttgccatttc
      661 ggtcgcatgc cctagcaggc caaaaccagt tacatcagtg gcagcatgaa cttcaaagga
      721 tgccataact tctgacgctg tcttgtctaa agtagtcata attgaagtca cttgctttat
      781 ctcttcagat gtgagaacac ttcgttttaa tgatgttgta taaatcccga ctccgatcgg
      841 ctttgtcaac actagccggt cgcctgtttt cgcaccggca tttgtccgga ttttatcagg
      901 gtggacggtt ccggtcactg caaggccaaa tttgggttcc tgatcgtcaa ttgaatgtcc
      961 tccgacaagc gtgacaccag cttccttcaa cttatcacct gctccgcgta aaatatcgga
     1021 aagtatgctt ttgtccaacg ttgaaatagg gaatgctacg atattaagag cagttatcgg
     1081 ctttccgccc attgcgtata catcgcttat tgcattcgtg gcagcaatct gaccaaaatc
     1141 ataagggtca tcgacaatcg gggtaaagaa gtcaagcgtt tgcacgattg cgatctcatc
     1201 ggtcagttta tatacgccag catcgtcact cgtatcaagg cttacgagaa gattgggatc
     1261 aaagactgcg ggaggaaggg attgcagcac ttgtgataaa tcggctggac cgattttaca
     1321 gccgcagcct cctttttttg ttaatgacgt cagttttaca gcattaggta ccttcatttg
     1381 cttgaccgcc tttcaatatg gataaaaata gtttagcaca ctggatacaa atgtatattg
     1441 tgtcatgttt aaaaaattgc tttctccgag atcaaaccat tggaagggaa tcgaataact
     1501 aataatggta catttatata aagatagttg aggaggttaa gagatgaata ctttttcgat
     1561 ggtcatcgat ggtaaagagg tagggaaaga tcttgagtcg atagatgtca tcaatcccgc
     1621 gacgggggaa tctatcgcaa ctgttccaga tggggggagg aaggaagcgg ttttagcagt
     1681 ggatgccgca tatgaagcat ttcaaaaatg gtccaaactt accacatatg agcgcagttc
     1741 acttttaaat aagtggcatg cgctgattga cttgcatatg gatgagctgg cacggacaat
     1801 gacggaagag caagggaaac cgttagcaga agcggtgggc gaaatgaagt acgcgaacgg
     1861 tttcatttcc tggtatgcag aagaagcaaa gcggatttac ggtgaaatga ttccatcaag
     1921 ccatactgga aaacggatat tcatccataa acagccggtc ggcgtaacag ctgtcataac
     1981 accatggaat ttccctgctg caatgatcac aagaaaagtg ggaccagcac tcgctgcagg
     2041 ctgtacagtt gtcatcaaac ccgccgaaca gactccgata acggcaatcc gattggctca
     2101 gctggcgttg gatgctggca ttcctgccgg tgtcattaat gtcgtgacag
//
LOCUS       DASULI010000170         6817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_720725, whole
            genome shotgun sequence.
ACCESSION   DASULI010000170 DASULI010000000
VERSION     DASULI010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720725"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFX34_03665"
     CDS             complement(<1..884)
                     /locus_tag="VFX34_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5564034.1"
                     /translation="MDTGTHIVMGAAICGLTLADPVVASDSVTMTAVFIGVVSGSLIP
                     DVDTVLKLRNNAVYIRNHRGITHSVPAVLLWPLVLSILLSFIFPEAAFYHLWAWTFLA
                     VFLHVFVDIFNAYGTQALRPFSNQWVAIGVINTFDPIIFALHLIAITAWMFGAEPVLT
                     FTLLYILVFFYYLLRFAVKAAVKHAVRNTIPDADDIFIAPTMHFFQWRVAASNKECHY
                     VGRAYGRSVNIYDRFEREPMPKSPYIDAAMEDPNIQAFVSFSPIYRWEVSYVSHLYEV
                     RLIDLRYRSKSYYPFVAVA"
     gene            1007..2077
                     /gene="mutY"
                     /locus_tag="VFX34_03670"
     CDS             1007..2077
                     /gene="mutY"
                     /locus_tag="VFX34_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX5564035.1"
                     /translation="MQLFERKLESEFRKALITWYLEEKRDLPWRRTSNPYHIWVSEVM
                     LQQTRVDTVIPYYERFIEKFPSLADLAYAKEEDVLKMWEGLGYYSRARNLQAGVKEVV
                     EEYGSRIPETRKEISKLKGVGPYTAGAVLSIAFGVPEHAVDGNVMRVLSRILLIEEDI
                     AIPRTRKIFEEVVMALVDKEDPSSFNQGLMELGATICTPRPKCLLCPVREFCTAFHEG
                     RQEELPVKSKKVKMKTVPMLSIVIQNTEGKWLLTQRPSKGLLANMWEFPMVESIPGDL
                     EEMTKNEFGITIGTITSLIEFTHVFSHLTWEMKSYLAVTDDAAPEGFEYFSAEEVEAL
                     PKAVPVMKIWDEAKNRFFVKKT"
     gene            2128..2328
                     /locus_tag="VFX34_03675"
     CDS             2128..2328
                     /locus_tag="VFX34_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004230295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-type small acid-soluble spore protein"
                     /protein_id="HEX5564036.1"
                     /translation="MTNKNRNKSNQQAPQASFSNPNKVKQQIMSEEFGAETDVNEVRQ
                     QNQQSQMNKTSGGAGGFENQSK"
     gene            2467..3015
                     /locus_tag="VFX34_03680"
     CDS             2467..3015
                     /locus_tag="VFX34_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX5564037.1"
                     /translation="MTIPKEGETIQVHSYKHDGNIHRVWQETTVLKGTRNIVIGANER
                     TLVTEADGRTWLTREPSICYFHAEHWFNIICMLREDGVYYYVNMSSPFVYDHQALKYI
                     DYDLDVKVFPDMSYLILDEDEYDDHKKKMNYPEVIDQILKRNMDKLLMWIKQRKGPFA
                     PDFIEVWTSRYQFHKQVKKNDQ"
     gene            3070..4815
                     /locus_tag="VFX34_03685"
     CDS             3070..4815
                     /locus_tag="VFX34_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564038.1"
                     /translation="MGESIKRYLKFVKPYNWQIILTILIGVVKFAIPLFIPYLIKIVI
                     DDIIGADALTDAEKTKQLFLWLGGTVLLFFIIRPPVEYYRQYFAQYVSNKILYDIREN
                     LYAHLQRLGLRYYSNTRAGEVISRVINDVEQTKNFVVIGLMNVWLDLATIVIATCIML
                     TMDVPLTIVTLLAFPFYAYSVKHFFGKLRDLTRKRSQALADVQSYLHERVSGISIIKS
                     FALEDKEQERFDETNGNFLDKAIDHTKWNAKAFAVVNTITDVAPLLVISYAGYQVING
                     SLSVGVMVAFIAYIDRLYNPLRRLVNASTSLTQSFASMDRVFELMEEPYDIKDKSGAK
                     ALPRIEGEIQFDHVGFSYGEAEEPVLRDVNFTVKPGETVALVGMSGGGKSTIVSLIPR
                     FYDVTHGSIRIDGYDLRDVQLKSLRDQIGLVLQDSILFSDSVKSNILMGNPDATEEEM
                     IAAAKAANAHDFIESLPQGYDTTVGERGVKLSGGQKQRIAIARVFLKNPPLLILDEAT
                     SALDLESEALIQESLERLASDRTTVIVAHRLSTITHADKIFVIDHGELKEVGNHEELM
                     AQEGIYYNLFQVQSI"
     gene            4966..5988
                     /locus_tag="VFX34_03690"
     CDS             4966..5988
                     /locus_tag="VFX34_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564039.1"
                     /translation="MNERKKILEVNDLQTTFFTDSGNVPAVDHIDFHLRDGEILGIVG
                     ESGCGKSVTSLSVMGLVPSPPGKITGGEIVYNGRNLIGLSEKEMRQIRGNDIAMIFQE
                     PMTSLNPLFTIGNQMVEAIMIHEKGWTKKKAEERAVEMLKLVGLPRADALMKDYPHQL
                     SGGMRQRVMIAMALVCDPKILIADEPTTALDVTIQAQILKLMKDLNVRLGTAILLITH
                     DLGVVAETCERVIVMYAGQIIEEAPTKLIFEDPQHPYTKGLIQSVPDMRIKKDRLHSI
                     PGNVPKPGSVKNGCRFAARCEFAFDRCFQENPELYETADVHQTRCFLYDEGRVKSDGH
                     KAFIES"
     gene            5960..>6817
                     /locus_tag="VFX34_03695"
     CDS             5960..>6817
                     /locus_tag="VFX34_03695"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5564040.1"
                     /translation="MATKPLLKVENLKKYFPVKKGMFGKTVGHVKAVDDVSFFVNEGE
                     TLGIVGESGCGKSTTGRVLMRLLEPTDGKIEFDGKDLASLSQEEMRKTRRDIQMVFQD
                     PYASLNPRHTIGKILEEPLIVHGLSDPKERKKKVHDFLEIVGLNAYHAKRYPHQFSGG
                     QRQRIGIARALMTNPKLIIADEPVSALDVSIQAQVLNLMQDLQKEFNLTYIFIAHDLG
                     VVRHISDRVAVMYLGKMVEVADSEQLYEKPLHPYTQALLSAVPVPDPDFQKEQIIIEG
                     DIPNPADPPT"
BASE COUNT         1931 a         1440 c         1789 g         1657 t
ORIGIN      
        1 gcaaccgcaa cgaaaggata atagcttttg ctccggtagc gcaaatcgat gaggcgtact
       61 tcatacagat ggctgacata ggagacttcc catctgtaga tcggcgaaaa cgagacgaac
      121 gcctgtatat ttggatcttc catggccgca tcgatataag gggatttcgg catcggctcg
      181 cgttcgaacc tgtcatatat attgacggaa cggccgtatg cacggccgac atagtgacat
      241 tctttattgg aagcggcaac ccgccactgg aagaagtgca tcgtcggcgc gatgaaaata
      301 tcatcggcat ctggaatcgt attccgaacg gcatgcttca ccgccgcttt gacagcaaaa
      361 cgcaataaat aatagaagaa aaccaaaata tacaaaagcg tgaatgtcag aaccggttcc
      421 gctccgaaca tccaagcagt gattgcgata agatgcaatg caaaaatgat cggatcaaat
      481 gtattgatga ctccgatggc gacccattga ttggaaaatg gccgtagcgc ctgtgtgccg
      541 taagcgttga agatatctac gaatacgtgt aaaaacacgg ctaagaacgt ccaagcccac
      601 agatgataga aagccgcttc ggggaaaatg aacgacagca ggatcgatag gacgagcggc
      661 caaagcagga cggcaggcac cgagtgggtg atcccgcgat gattccgtat ataaaccgcg
      721 ttattcctca acttaaggac agtatcgacg tcgggtatca aagagcccga tacgacgcca
      781 atgaacaccg ccgtcattgt gacggagtct gaggcaacga ctggatccgc caatgtcaaa
      841 ccgcaaatgg cggcacccat gacaatatgt gttccggtat ccaaaaaatc actcctttac
      901 atcgcctctg agtcggcaat tgaacatagt tgtacaatta cagttctaca ttaagtatat
      961 accctatttt tcattaacga aaagcatatg caaaacggag gtattcatgc aactttttga
     1021 acggaagctt gaaagtgaat tcaggaaagc attaatcacg tggtatttgg aagaaaagag
     1081 ggatctgccg tggagacgga cgtcaaatcc ttatcatata tgggtgtctg aagtgatgct
     1141 acagcagacg cgtgttgaca cggtcatccc ttattatgaa cggtttatcg aaaaattccc
     1201 gtcgcttgcc gatttggcgt atgcgaagga agaagatgtt ttgaaaatgt gggaaggact
     1261 cggatattat tcccgcgccc ggaacttgca agccggcgtg aaggaagttg ttgaagagta
     1321 tggcagccgg attcccgaaa cgcgaaagga aatttccaag ttgaaaggtg taggcccgta
     1381 tactgcggga gcagtgctca gcatcgcttt cggcgttcct gagcatgctg tcgacgggaa
     1441 tgtgatgagg gtgctgtcac ggattttatt gattgaagaa gatatcgcga ttccgcgcac
     1501 gcgaaaaata ttcgaggaag tcgtcatggc gttggttgac aaggaagatc cttcatcttt
     1561 caatcaaggc ttgatggaac ttggtgcgac gatctgcaca ccgcgtccga aatgtctcct
     1621 ttgcccggtc cgtgaattct gcacggcgtt ccacgaaggc agacaggaag aattgccggt
     1681 gaaatcaaag aaagtgaaga tgaagacagt gccgatgctt tccattgtca tccagaatac
     1741 cgaagggaaa tggctgttga cgcaacggcc gtctaaagga ctgctagcga atatgtggga
     1801 atttccgatg gttgaaagca ttccggggga tttagaggaa atgactaaaa atgaatttgg
     1861 aataacgatc ggaacaatta cttcactgat cgaattcacg catgtctttt cacatctcac
     1921 ttgggaaatg aaaagttatc ttgccgtgac agatgatgca gcgcctgaag gctttgaata
     1981 cttttcggct gaagaggtcg aagcgcttcc gaaagcggtg ccagtcatga aaatttggga
     2041 cgaagcgaag aatcgatttt ttgtcaaaaa aacatagaat aaaacggacc gctttgaaca
     2101 tcatagtggt tacggaggtg aaaatccatg acaaacaaaa atcgaaacaa gtctaaccaa
     2161 caagccccgc aagcatcttt ctcgaaccca aacaaagtga aacaacaaat catgtctgaa
     2221 gagtttggag ccgaaacaga tgtgaacgag gttcgccaac aaaaccagca atcacagatg
     2281 aacaaaacat ctggtggagc tggcggtttc gaaaatcagt ctaagtaatg gatttacatc
     2341 acctgccggc agcttaagct gccggttttt ttgtttatac cgaagcgtaa acgtttcaat
     2401 tttgattggc caattggtat aatgtacgaa tattagggtt tccgacagtt aaaggtggtt
     2461 ttactaatga caataccgaa ggaaggcgaa acgatacagg tacatagcta caagcatgac
     2521 ggcaatatcc accgtgtttg gcaagaaacg actgtgttga aggggacacg gaatattgta
     2581 atcggagcga acgaacggac gctcgttacg gaagcggacg gaaggacatg gctgacaagg
     2641 gagccttcga tctgttactt ccatgcagaa cattggttca atatcatttg catgctgcgg
     2701 gaggacgggg tctattatta cgtgaacatg agttcaccct tcgtctatga ccaccaagcg
     2761 ttgaaatata tcgattacga cctcgatgtg aaagtgtttc cggatatgag ttatttgatt
     2821 ttggatgaag atgaatatga tgatcataaa aagaagatga attatcccga agtgatcgat
     2881 caaatattaa agcgcaatat ggataaactg ctcatgtgga tcaaacagcg caaagggcca
     2941 tttgcaccgg acttcatcga agtatggact tccaggtatc agttccataa acaggtaaag
     3001 aaaaatgatc aatgagagcc tgtctgcgtt tatgcagttg ggctttttat tgtttgaagg
     3061 aggatggaaa tgggagaaag tataaaacga tatttgaaat ttgttaagcc atacaattgg
     3121 caaatcatct tgacgatttt aatcggggtc gtcaaatttg ctataccttt gttcattcca
     3181 taccttatta aaattgtcat cgacgatatt atcggggcgg atgcgctgac cgatgcggag
     3241 aagacaaagc agcttttcct atggctcggg gggactgtat tactgttttt catcatcagg
     3301 ccgcctgttg aatattatcg ccaatatttc gcgcaatacg tgagcaataa aatcctgtac
     3361 gatatccgtg aaaatcttta tgcacatttg caaagactcg ggttgcgcta ttattccaat
     3421 acgagggcgg gcgaggtcat ctcgcgtgtc atcaatgatg tcgaacagac gaagaatttt
     3481 gttgtcatcg gcttgatgaa tgtatggctc gacttggcaa cgattgtcat tgcgacatgc
     3541 atcatgctga cgatggatgt tccgctgacg attgtgacat tgctcgcatt ccctttttat
     3601 gcatacagtg tgaagcattt cttcgggaag ctccgggatc tgacaaggaa acgttcgcag
     3661 gcgcttgcag acgtgcaaag ttatttgcat gaacgggtgt cgggaatcag catcatcaaa
     3721 agcttcgcgc ttgaggataa agagcaggag cgatttgatg aaacgaacgg caatttcctt
     3781 gataaggcca ttgatcatac gaagtggaat gcgaaagcgt ttgcggtcgt caatacgatt
     3841 accgatgtcg ccccgttgct cgtcatttcc tatgcgggct atcaagtgat caacggttct
     3901 ttgtctgtcg gtgtgatggt cgctttcatt gcgtacatcg atcgtctgta caatccattg
     3961 aggcgattgg tcaatgcttc aacgtcattg acgcaatcgt tcgcttcgat ggatagggta
     4021 ttcgagctga tggaggagcc ttacgatatt aaggacaaga gcggtgccaa agcgttgccg
     4081 cggatcgaag gggaaatcca attcgatcat gtcggctttt catatggtga agctgaggag
     4141 ccggtgctgc gtgacgtcaa cttcacggtc aaaccgggtg agacggtggc attggtcggc
     4201 atgagcggag gaggcaagtc aacaatcgtc agcctgattc cgcgatttta tgatgtgacg
     4261 catggctcga tccggatcga tggctatgac ttgcgtgatg ttcaattgaa atcattgcgt
     4321 gaccaaatcg gccttgttct ccaggacagc atcctcttca gcgactcggt gaagagcaat
     4381 attctaatgg gcaatcctga cgcgaccgaa gaagaaatga ttgccgccgc caaagctgca
     4441 aatgcgcatg atttcatcga atcgctgccg caagggtatg acacgaccgt cggagagcgg
     4501 ggcgtgaaat tatcgggcgg acagaaacag cggatagcaa tagcccgtgt attcttgaaa
     4561 aatccgccgc tgctcatttt ggatgaagcg acatccgcac tggaccttga aagtgaagcg
     4621 cttatccaag agtctcttga aaggcttgcg agtgacagga caaccgtgat tgtggcgcac
     4681 cgactatcga cgatcaccca tgcggacaag atctttgtca tcgatcatgg ggaattgaag
     4741 gaagtcggga atcatgaaga actcatggcg caggaaggca tctattacaa tctgtttcag
     4801 gttcaatcga tataaatcgt gaagctaccg gagatccggt agcttttttg tgtccttgaa
     4861 tgatccatgt ctctatgaaa atttcatatt gccttttccc taatagaaag tataatggat
     4921 ataatttaac gtttctgaaa acatataaaa cgaaaaggtg atctgatgaa tgaaaggaag
     4981 aaaatcctcg aggtaaatga cttgcagact acctttttca cagactccgg caatgttccg
     5041 gcagttgatc acatcgattt tcatctccgg gacggcgaga ttttaggaat cgtaggggaa
     5101 tcgggatgcg gaaaaagtgt aacctcctta tccgtcatgg ggcttgtgcc aagtccgccg
     5161 gggaaaatca ccggtgggga aattgtgtat aacggacgca atttgattgg cctgtcggaa
     5221 aaagagatgc ggcagatccg cggaaatgac atcgcgatga tttttcagga gccgatgacg
     5281 tcgttgaatc ctctatttac aatcggcaac cagatggtcg aagcgattat gatccatgaa
     5341 aagggatgga cgaaaaagaa ggcggaggaa cgggccgtcg agatgttgaa gttggtcggc
     5401 ttgccacgtg ccgatgcgtt gatgaaggac tatccacacc aattgtcggg cggcatgcgg
     5461 caaagggtca tgattgcgat ggcgcttgtc tgcgatccta aaatattgat agcggatgaa
     5521 ccgactacgg cgctagatgt gacgattcag gcgcaaatat tgaaactgat gaaagatctg
     5581 aatgtccggc tggggaccgc catattattg attacccatg atcttggggt cgttgcagaa
     5641 acttgtgagc gggtcatcgt catgtatgca gggcaaatca tcgaagaggc cccgaccaag
     5701 ctcatctttg aagatcccca gcatccgtat acgaaaggcc tgatccagtc agtgccggat
     5761 atgcgcataa aaaaagatcg acttcattcc attcccggga atgtgccgaa gcccggttcc
     5821 gtcaagaacg gctgcaggtt cgccgcgcgg tgcgaatttg cattcgaccg gtgctttcag
     5881 gaaaacccgg agctttatga aacagcggat gtccatcaga cgcgatgttt cctatacgac
     5941 gaaggaaggg tgaagtcgga tggccacaaa gcctttattg aaagttgaaa acttgaagaa
     6001 atactttccc gttaaaaaag ggatgttcgg gaagacggtc ggccatgtga aggctgtcga
     6061 tgacgtctcg tttttcgtga atgaggggga gacccttgga atcgtcggtg aaagcggttg
     6121 cggaaaatcg acgactggtc gtgtactcat gcgtttgctg gaaccgacag acggaaaaat
     6181 cgagtttgac gggaaagatc ttgcttcatt aagccaggaa gagatgagaa aaacacggcg
     6241 cgatatccag atggtattcc aagatcctta cgcgtcgttg aaccctcgcc atacgattgg
     6301 caagattttg gaggagccgc tcatcgtcca tggcttgtcg gacccgaagg aaaggaagaa
     6361 gaaggtccac gattttttgg aaatcgtcgg attgaatgcc taccatgcga agcgctatcc
     6421 gcatcagttc agcggagggc agcggcagcg gatcggcatt gcacgggcct tgatgacgaa
     6481 tccgaagctg attatcgcgg atgagcccgt ctcggcgttg gatgtttcca tccaagccca
     6541 agtattgaac ctcatgcaag atctccaaaa ggagttcaat ctcacctata ttttcattgc
     6601 ccatgacctt ggcgtcgtcc ggcatattag cgatcgcgtc gctgtcatgt atttaggaaa
     6661 gatggtggag gttgcggata gtgagcagct ttacgaaaag ccgctgcatc catatacaca
     6721 agcgttatta tcggctgtac ctgtgccgga cccggatttt cagaaggaac agatcatcat
     6781 agaaggagac atccccaatc cggcggatcc gccaacc
//
LOCUS       DASULI010000171         4840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1795106,
            whole genome shotgun sequence.
ACCESSION   DASULI010000171 DASULI010000000
VERSION     DASULI010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795106"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..195
                     /gene="rrf"
                     /locus_tag="VFX34_03700"
     rRNA            97..195
                     /gene="rrf"
                     /locus_tag="VFX34_03700"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(321..821)
                     /locus_tag="VFX34_03705"
     CDS             complement(321..821)
                     /locus_tag="VFX34_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564041.1"
                     /translation="MKRKSLAFIFSTLLILMVGCSENETVKSKFILPEDVPDFVAESD
                     LENIDWDKKAISFSRNIIGNENRSGVIGADMPSITTKQKWMWHLWGIEDPAATNLTVV
                     GLHRASGTVHPILTTGWTTGLAGPNNGADAHAPSNVQIPNAGEWAILLYTDEELFDVL
                     IYEINE"
     gene            966..1301
                     /locus_tag="VFX34_03710"
     CDS             966..1301
                     /locus_tag="VFX34_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564042.1"
                     /translation="MKKALIVILMLLALISAYQYQKPILTTEEAILQAYEYLLNPPEG
                     YGDDLIPTKELEEMWPTHTRLTMKHGFYSEMVNRRSWEVTFRYDGKEPMVILDAITGE
                     FIMIYGPLN"
     gene            1486..2907
                     /locus_tag="VFX34_03715"
     CDS             1486..2907
                     /locus_tag="VFX34_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5564043.1"
                     /translation="MNVQTKPLVHALSQFKEKRPISFHVPGHKNGLLSGLPEEMQSVL
                     QYDLTELEGLDDLHEPSGVIREAEQRLSALYQSDRSFFLVNGSTVGNLAMMYAACTPG
                     DTVIVQRNAHKSIFHAIELTGAKPVFVSPAWDSHSNTPGTVRFAQVKEAIKAYPHAKA
                     VVLTYPTYYGVTGSELTDIIQFSHAKGIPVLVDEAHGAHFVTGDAFPPSALELGADVV
                     VHSAHKTLPAMTMASFLHIRSTIISPERIAHYLGMFQSSSPSYLLMASLDDARAYAES
                     YTKEDQAEFLEKRRTFIDDLFVIPALKIIETDDPLKLLLRVQGYTGFELIARLEAEGV
                     FAELADPFQVLFVLPLLKKGKEYPFSESVQKIAAAVAGLPEKEEMAGNESIVMDEGLS
                     TLTYLPQELLGRDTAWKSIEESVGHVAAASIIPYPPGIPLLIGGETVTVRHIASLLQY
                     LQMNAKFQGAIRANARQIQVIID"
     gene            2920..3558
                     /gene="tmk"
                     /locus_tag="VFX34_03720"
     CDS             2920..3558
                     /gene="tmk"
                     /locus_tag="VFX34_03720"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5564044.1"
                     /translation="MLNKGIFITFEGPEGAGKTTVIAEIYKRLEQEGKKAILTREPGG
                     IRISEKIRGIIHDNEHQEMDGMTEALLYAAARRQHLVEKVVPAIKEGQIVLCDRFIDS
                     SLAYQGYARGLGMENVMTINSFAIGETMPDLTIFFDITPEEGLRRISNSGEREQNRLD
                     NETLQFHEKVYEGYQLLLKEFPDRIVSIDASKPLSEVTENVWKIISSQLINK"
     gene            3762..4091
                     /locus_tag="VFX34_03725"
     CDS             3762..4091
                     /locus_tag="VFX34_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX5564045.1"
                     /translation="MKLIVAVVQDQDSNRLSAALTKNDFRATKLASTGGFLRSGNTTF
                     LIGTDDSLVPKALELIRENCRSRDQMVAPVSPMGGNADSYIPYPVEVEVGGATVFVLP
                     IEQFHHF"
     gene            4112..4552
                     /locus_tag="VFX34_03730"
     CDS             4112..4552
                     /locus_tag="VFX34_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HEX5564046.1"
                     /translation="MKVNGDYRTGPDKFRNDPLRSTQGNARFGQMVLKQEERLHGEQI
                     TRLLGDISSAGDRLARSRNLRDMAKFKMLVRRFLKESVESGMGLKQSHTWNQFGEGRR
                     LNIVQTIDEKLIELAEALLNDEQTSVELLGKIGEIKGLLINIYR"
     gene            4594..>4840
                     /locus_tag="VFX34_03735"
     CDS             4594..>4840
                     /locus_tag="VFX34_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX5564047.1"
                     /translation="MENSIRQRQKAVIDRLEATFRNNRIGHAYLFDGEAGTGKEEAAI
                     HFAKLLLCESPENAVPCETCRNCKRVASGNHPNVTKIE"
BASE COUNT         1381 a         1078 c         1182 g         1199 t
ORIGIN      
        1 aggacttaac cttgaagtta agaagtacgg ttgaacttga gttcagcgac aatgttggtt
       61 ttatccggtt ttgaacgaac aagcatcgtt caatcgtctg gtgatgatgg cgaagaggtc
      121 acacccgttc ccataccgaa cacggaagtt aagctcttca gcgtcgatgg tagtaaggcg
      181 gcgcagccgc tttgcgtaat ttacgccaac ggtgtaaatt acgcagggaa caggcttccc
      241 cctgtgagag tagaacgtcg ccgggccaag gccatcccct gtgggggatg gtttttttgt
      301 gtttaaaata ccttctggtt tcattcattt atttcataga tcaatacatc aaacaattct
      361 tcatcggtat aaagcaatat cgcccattca ccagcattag ggatctgtac attggaaggg
      421 gcgtgagcat ctgctccgtt attcggccct gcaaggcctg tcgtccaacc tgtcgttaaa
      481 ataggatgga cagtgcccga agcacgatgg aggccaacga ctgttagatt ggtcgctgca
      541 ggatcctcaa tcccccaaag atgccacatc catttttgtt ttgtcgtaat gcttggcatg
      601 tcggcgccaa tcacgccgga tctgttttcg ttaccgataa tattacgact gaatgaaatg
      661 gcttttttat cccaatcaat attctccaaa tcactttctg ccacaaagtc cggcacatcc
      721 tcaggcaaaa taaatttgct tttaactgtt tcattttccg agcaaccaac cattaggata
      781 agcaaagtgg aaaaaataaa agctaaagat tttcgtttca aaagttatcc cgccccttca
      841 ttagttatca atttcaaata acggtacgtt ttcaatggta tataagtttc agtaacgatc
      901 gctattgcgg aaagatactg agtagttcat gattagatta ctagagaaag aatggagcaa
      961 atcgaatgaa aaaagccctc atcgttattc ttatgctact cgctctcata tcagcctatc
     1021 aataccaaaa gccgattcta actacggaag aagcgatttt acaggcgtat gaatatttat
     1081 tgaatccgcc agaaggatat ggcgacgact tgatacctac taaagagtta gaagaaatgt
     1141 ggcccactca tacaagatta acgatgaagc atgggttcta ctctgaaatg gtcaaccgcc
     1201 gatcatggga agtgaccttt cgatatgacg gcaaggaacc tatggtaatc ttggatgcca
     1261 taaccggtga attcataatg atatatggtc cactgaatta ggtggagtat gatgtcgtac
     1321 tttgtattac tcggttttgc cgctgtattc ctttccatcg gcttgtcgag aatgaaatat
     1381 gtctaaggcc gggaccccct cggctttttt catttcgaag aaggagtttt cacccttagt
     1441 catgcgactt cctttataat ggatacaaag gaagtgtgaa ttctaatgaa tgtacagact
     1501 aaaccgcttg tccatgcact gagccaattc aaggaaaagc ggccgatctc ctttcatgtg
     1561 ccgggtcata aaaacggttt gctgtccggg ttgcctgaag aaatgcaatc cgtgctgcaa
     1621 tatgacttga cggaattgga agggctggat gatttgcatg agccgtctgg agtgatccgt
     1681 gaagcggagc aacgactttc agctttatat caatccgacc ggagcttctt tctcgtcaat
     1741 ggttctacgg tcggcaattt agcgatgatg tatgcggcat gcacgcctgg cgatactgtc
     1801 attgtgcagc ggaatgcaca taaatcgatc ttccatgcga tcgaattaac aggcgctaag
     1861 ccggtctttg tatcgcccgc ttgggattcc cattcgaata cgccgggtac agtccgattt
     1921 gcacaggtga aggaggcgat taaggcctat ccccatgcca aggcagttgt tctcacctat
     1981 ccgacctact atggggtgac gggctcagag ttgacggata tcattcaatt cagccatgcg
     2041 aagggcatac ctgttttagt ggatgaagcg catggtgccc attttgtcac aggagatgca
     2101 tttccaccct ccgcattgga gctgggggcg gacgtcgtcg tacactcggc ccataaaaca
     2161 ttgcccgcca tgacaatggc atcattttta catatcagat cgactattat ctctcctgaa
     2221 aggattgcgc attacttggg catgttccaa tcgagcagcc cctcctattt gctcatggcg
     2281 tctctcgatg acgcacgggc gtatgccgag tcgtacacga aggaagatca agctgaattc
     2341 ttggagaagc gccgaacttt cattgacgac ctgttcgtca ttccggcatt gaaaatcatt
     2401 gaaacggacg atccattaaa gctattgctg cgagtgcaag gctatacagg gttcgaattg
     2461 attgcaaggc ttgaagcgga aggcgttttc gccgaacttg cggatccttt ccaagtgcta
     2521 ttcgttctgc cattgctgaa aaagggtaaa gagtacccat tcagtgaaag cgtgcaaaaa
     2581 attgccgctg cagttgcagg cttgccggag aaggaagaaa tggcggggaa tgaatcgata
     2641 gtgatggatg aaggattatc gacgctgacc tatctcccgc aagaacttct tggacgtgat
     2701 actgcatgga aatcgattga agagtctgtc gggcatgtgg cggcagcatc catcattcct
     2761 tatccgcccg gcatcccact cctcatcggc ggggaaacag tgacagtgag gcatatcgca
     2821 tcgcttttgc aatacttgca aatgaatgcg aaatttcaag gcgccattcg ggcaaatgct
     2881 agacaaatac aagtgatcat cgattgacgg aggcatacaa tgttgaataa agggatattt
     2941 atcacctttg aaggtccaga aggtgcagga aaaacgactg tgattgcaga aatatataag
     3001 aggctggagc aggaaggcaa aaaagccatc ctgacccgtg aacccggagg catccgcatc
     3061 tcggaaaaaa tccgcggaat cattcatgac aatgaacatc aggaaatgga cggcatgacg
     3121 gaggcgctcc tatatgccgc agcacgcagg caacaccttg tcgaaaaagt tgtaccggca
     3181 atcaaagaag gacaaatcgt cctctgcgac cgtttcatcg acagctcgct cgcctaccaa
     3241 ggctatgcaa gaggactcgg catggagaat gtcatgacga tcaactcatt cgccatcgga
     3301 gaaacgatgc cggacctaac catcttcttc gacatcacac cggaagaagg actccggaga
     3361 atttccaaca gcggcgaacg ggaacaaaac cgcctggaca acgaaacctt acagttccac
     3421 gaaaaagttt acgaaggcta ccaattactg ctaaaagagt tcccggatcg catcgtcagc
     3481 atagatgcaa gcaaaccatt atctgaagta actgaaaatg tttggaaaat tataagttct
     3541 caactcatta ataaatagga ttcatgatat aatgtaggag agggctttta gcttctcgat
     3601 ttggaacttg attgtttatt aataagcatt tgccgttgat tgaagcgaaa gccgtaacga
     3661 agaacggctt ttgcgagtaa aagtgcagcg ttacgagcac cggtgtagcc ggaaatagta
     3721 ttcgtttcta taaaatataa aagctaaaag gagagggtac cgtgaaattg attgtggcag
     3781 ttgtacaaga ccaagatagc aaccgcttat cggcggcttt aacgaaaaat gattttcgcg
     3841 ctacgaaact agccagtact ggagggtttc tgcggtcagg aaacacaacc ttcctcatcg
     3901 gaacggacga cagcctcgtt ccgaaagctt tggaattaat ccgtgaaaat tgccgttcgc
     3961 gcgatcaaat ggtcgcaccc gtctcaccga tgggcggcaa cgccgattct tatatcccct
     4021 acccagtaga agtggaagtg ggcggcgcca cagtatttgt cctgccgatt gaacaatttc
     4081 atcatttctg atgaaatgag gtgatgtaga aatgaaagtg aatggagact accggaccgg
     4141 gccagacaag ttccgaaacg atccattgcg ctcaacgcaa gggaacgccc ggttcggtca
     4201 aatggttctg aaacaggaag aacgcctgca tggggaacaa atcacaagac tgctcggaga
     4261 catctcctca gcaggtgacc gtcttgcccg ttcacggaat ttaagggaca tggcaaaatt
     4321 caagatgctt gtccgccgtt tcctgaaaga gtcggttgaa tcgggaatgg gcttgaaaca
     4381 atcccatacg tggaatcaat tcggcgaagg acgacgcttg aacattgtcc agacaatcga
     4441 cgagaagctt attgaactgg cagaagcctt attgaatgat gagcagacgt cagtcgaatt
     4501 attagggaag atcggggaaa tcaaaggact tctcattaat atatatagat gacccgtgta
     4561 tgtacacgtt ttttgcagca aggagggaca agcgtggaaa acagcatcag gcagaggcaa
     4621 aaagccgtca tcgacagatt ggaagcgaca ttccggaata accggattgg acacgcctat
     4681 ctattcgatg gtgaggcggg cacgggaaaa gaagaggcag ccatccattt tgcgaagctt
     4741 ctgctttgtg aaagtcccga aaatgctgtt ccatgtgaaa catgtcgaaa ctgcaaacgg
     4801 gttgcgtcag ggaatcatcc gaatgtgacg aaaatcgaac
//
LOCUS       DASULI010000172         5180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_533586, whole
            genome shotgun sequence.
ACCESSION   DASULI010000172 DASULI010000000
VERSION     DASULI010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533586"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="ruvB"
                     /locus_tag="VFX34_03740"
     CDS             complement(<1..854)
                     /gene="ruvB"
                     /locus_tag="VFX34_03740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010303555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5564048.1"
                     /translation="MERVISSEQSNYDDSFEQSLRPQTLKQYIGQETVKDNLGIFIQA
                     AKGRSESLDHVLLYGPPGLGKTTLATVIANEMGVNVKMTSGPAIERPGDLAAVVSSLE
                     PGDVLFIDEIHRLNRSIEEVLYPAMEDFCLDIVVGKGPSAKSIRLDLPPFTLIGATTR
                     AGSLSAPLRDRFGVPLRLEYYGEEPLKEIVIRSASLFDVDIDANAAVEIARRSRGTPR
                     IANRLLRRVRDYAQVRGDGNITMAIAKEALEMLQVDSHGLDHIDHKLLVSMIERFRGG
                     PVGIDTIAA"
     gene            complement(885..1490)
                     /gene="ruvA"
                     /locus_tag="VFX34_03745"
     CDS             complement(885..1490)
                     /gene="ruvA"
                     /locus_tag="VFX34_03745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5564049.1"
                     /translation="MYDYIKGFITRVTPEYITLEQGGIGWQIMTPNPYAFHQTEEIQQ
                     VFTYLHVREDVQWLLGFKSLEQRELFKKLITVSGIGPKGALAILASGIPSQVIGAIER
                     EDEAFLVQFPGVGKKTARQMILDLKGKLGDLFTEIEMPDDEVNLLTMAENGALEEAIL
                     ALQALGYSQRELAKVKTEMQKEELDTEGYMKLALRLLLKQK"
     gene            complement(1596..2318)
                     /locus_tag="VFX34_03750"
     CDS             complement(1596..2318)
                     /locus_tag="VFX34_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5564050.1"
                     /translation="MAGHSKWKNIQNRKGAQDAKRGKIFQKMSRELYVAAKSGGGDPE
                     MNPALRLAIEKSKGFNVPNDVIKRAIDKATGAGADENYEEVVYEGYGPGGVAVLVQCL
                     TENRNRTGPNIRVAFNKNGGSLGESGSVGYLFNRKGRLFVERTKEADEEAIMLAALEA
                     GAEDIESTENGFEIITEPSGFMEVKEALEAEGIEFISAEVEMIPTIYTELHGDIADQF
                     EKMIDALEDDDDVQNIYHNASE"
     gene            complement(2330..3217)
                     /locus_tag="VFX34_03755"
     CDS             complement(2330..3217)
                     /locus_tag="VFX34_03755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX5564051.1"
                     /translation="MVADRRMALEKDFRKVKKNVWKMVEGGAAYSLKRYTSRSTAVKV
                     EYVHQQLHGIRFPHIVPLIPTEDPLFIMQPWIEGTKPVNFRRRSDRTDSLSALQALHK
                     TKEQIDWDASPYLHRYQLLDKWRERLDRFIDNRDASGSFLRKQAVDEIIMYGEEALPI
                     VKKNYRKLSGQTLLHGDVVHHNILRDGNGLIRFIDFDLACTGPPGTELALWMHRVLPN
                     VCYNLEFLLSEQSALGDLDAASMSMLLYPNELLREWLHLLSMPEAKRENLARKLIPFT
                     EYALSSWPKLWYDIERNMK"
     gene            complement(3207..4370)
                     /locus_tag="VFX34_03760"
     CDS             complement(3207..4370)
                     /locus_tag="VFX34_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5564052.1"
                     /translation="MDVHIVVKGDTLWKIARQYGIPFEDLKRVNAHLANPDYIVPGMK
                     IFLPKKKTEHMPSGKGKEKMPEKVKQPQAPPQAHEKPGKPKEPMPLPKEKFEMPKPPI
                     PSAPVQKPPAAMTPPAESPMQTLSTVPSLPPMPPPVPMPPMQMPPMQMPQFFQPCMPV
                     PCGWMPIYDADCAPMTFPSFTQQMPAPVMPQQVMPTQEMPMPPAQLGVKPPEIDMTEE
                     LEEFEESPIYPGRGPGVSPPYPIRGWELMESPTEPMESPEQMDHFPPEQGYVPQMVSP
                     EFQNPYGMMQQQPYAFGHPCMPMHHFGCGGCGCGCPPQGFYGVGPNEMQPNFMMPMQV
                     SPMQMHPSQMYPMPMNQMPMNQMPMNQMPMNPMHVNPMQMQPFNPMDNFGCGC"
     gene            complement(4545..>5180)
                     /gene="pheA"
                     /locus_tag="VFX34_03765"
     CDS             complement(4545..>5180)
                     /gene="pheA"
                     /locus_tag="VFX34_03765"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5564053.1"
                     /translation="AEISTPIEQHLMVNKIRKDESGKLASIHSHPVALAQCHKYLQYH
                     YRRTPLIQTTSTAAAAKYISENPELCIAAIGNSLAAEKYGLHIAEKNIHDFHFNHTRF
                     VVLSMRKGQLEEQGHSELPKTTFMVKLPEDDRSGVLHQILSVFAWRKLNLSKIESRPM
                     KTGLGNYFFIIDVLESEEHPMMKGALEELAALNVAVRSFGSYFSYEQRPSR"
BASE COUNT         1188 a         1370 c         1175 g         1447 t
ORIGIN      
        1 gcagcgatag tgtcaatccc gaccggcccg ccacggaacc gttcaatcat gctgacgagc
       61 aatttatggt cgatgtgatc gagaccgtgt gaatcgacct gcagcatttc aagcgcttcc
      121 tttgcgatcg ccattgttat atttccgtct ccacgcactt gggcgtaatc ccgcactctt
      181 cgcaggaggc gattggcaat gcggggcgtt cccctcgatc gtcttgctat ttcaaccgcc
      241 gcgttggcat cgatatcgac atcaaatagg cttgcgcttc ggatgacgat ttccttcaac
      301 ggctcttctc cgtaatattc gagtcggagc ggtacgccga aacggtcacg aagcggtgca
      361 gacaaagaac cggctctcgt cgtcgctccg attagcgtga atggcggcag gtccagccgg
      421 atcgatttgg cagaaggacc ttttccgaca acgatgtcga gacaaaagtc ttccattgcc
      481 ggataaagca cttcctcgat cgaacggttc aatcggtgaa tttcatcgat gaacaggacg
      541 tcgcccggtt ccaatgaaga tacgacagct gccaaatccc ccggccgttc aatcgccggc
      601 ccgctcgtca ttttcacatt gacgcccatt tcgtttgcaa tgaccgttgc aagtgtcgtt
      661 ttaccaaggc ctggaggacc atataaaagc acatgatcca agctttcact tctgcctttt
      721 gcggcttgga tgaaaatgcc cagattgtct ttcacggtct cttgtccgat atattgcttc
      781 aacgtctgag gtcgcaaaga ttgttcgaag ctgtcatcat aattggattg ttcacttgat
      841 atgacccttt ccatacgtag cgcccctcct ttctttttat actattattt ttgcttcaac
      901 agcaaacgca atgcaagttt catataacct tccgtatcca gttcttcctt ctgcatttct
      961 gttttcactt ttgctagttc gcgctgtgag taaccgagtg cttgcaacgc aaggatagct
     1021 tcttccaatg ctccgttctc cgccatcgta agaaggttca cctcgtcgtc cggcatttcg
     1081 atttccgtaa atagatctcc aagcttccct ttcaggtcca ggatcatttg acgggccgtc
     1141 tttttcccga cacccggaaa ctggacgagg aatgcttcgt cttccctttc gattgcaccg
     1201 atcacttggg acgggatgcc actggccaat atggcgagag cccctttcgg accgattccg
     1261 gagactgtga tgagcttctt gaagagttca cgctgttcga gtgatttgaa gccaagcagc
     1321 cattgcacat cttcacggac gtgcaaatag gtgaacactt gctggatctc ttcggtttga
     1381 tggaatgcat atggatttgg cgtcatgatc tgccagccga tgccgccttg ttcgagcgtt
     1441 atgtattcgg gtgtaacccg tgtgatgaat cctttaatat aatcgtacaa gtctttctcc
     1501 ccctaaagta tcgaaactat tatagcatac gaacgggttt tctacctatc gatgtgaata
     1561 gcgccgcaaa taaaaaaagc cgcagacgag gcggctcatt cacttgcgtt atgataaatg
     1621 ttttgcacgt catcatcgtc ttccagtgca tcaatcatct tctcgaattg gtcggcgatg
     1681 tcaccatgca attccgtata gatcgtcggg atcatttcca cttccgctga aatgaattca
     1741 attccttcgg cttccagggc ttctttcact tccatgaagc cggatggttc cgtaatgatt
     1801 tcaaagccgt tttctgtcga ctcaatatct tccgctcccg cttccagtgc cgccagcata
     1861 atcgcctctt catcggcctc cttcgtacgc tcaacgaaca agcgtccctt acggttgaaa
     1921 agatagccga ctgagccgct ttctcccaag ctgcccccgt ttttattgaa agctacacgg
     1981 atattcggcc ctgtcctgtt ccggttctca gtcagacatt gcacaagaac cgctacccct
     2041 ccaggtccat acccttcata gacgacttct tcatagtttt catccgcacc tgcgccggtc
     2101 gctttatcga tagccctttt aatgacatca ttcggcacgt tgaagccttt ggatttctcg
     2161 attgcaagac ggagcgcagg attcatttcg ggatctcctc cgcctgactt ggcagctaca
     2221 tagagttccc tcgacatctt ttgaaagatt ttacctcgtt ttgcgtcttg cgcaccttta
     2281 cggttctgga tatttttcca ctttgaatgg cctgccatta tgcttcctcc tacttcatgt
     2341 tccgttctat atcataccat agcttcggcc acgaagagag agcatattcc gtaaagggga
     2401 taagcttcct cgcgagattt tcccgctttg cttccggcat gctcaatagg tgcagccatt
     2461 ccctcagcaa ttcattcgga taaagcaaca tggacatcga tgctgcatcc agatcgccca
     2521 aagcggactg ttccgacaga aggaactcca agttatagca gacattcggc aatacgcgat
     2581 gcatccaaag cgccaattcg gttccagggg ggccagtgca ggcaaggtcg aaatcgatga
     2641 agcggatcag cccgtttcca tcgcgaagta tattgtgatg gacgacatcc ccatgcagga
     2701 gcgtctgacc cgataacttc cggtaattct tcttcactat cggaagggct tcctctccat
     2761 acatgatgat ttcgtcaacc gcctgtttcc ttaagaaact cccggatgca tctcgattat
     2821 cgatgaaccg atccaatctt tcccgccatt tatccaggag ctgatatcta tgaagataag
     2881 gggaggcgtc ccaatcgatt tgctccttcg ttttgtgcaa tgcttgaagg gccgataagg
     2941 aatcggtccg atcgcttctt cgcctgaaat tcacgggctt cgtcccttca atccatggct
     3001 gcatgatgaa caatggatcc tccgtcggaa tgagcggaac gatatgtgga aatcggattc
     3061 catgcaactg ctggtgtaca tactccactt taacggcggt tgaccgggac gtatacctct
     3121 tcagcgaata ggccgctccc ccctccacca tcttccagac attctttttt actttccgga
     3181 aatccttttc cagcgccata cgccggtcag caaccacacc cgaaattatc catagggttg
     3241 aatggctgca tttgcattgg gttcacatgc atcggattca tcggcatttg gttcattggc
     3301 atttgattca ttggcatttg attcattggc atcggataca tttgtgaagg atgcatttgc
     3361 atcgggctga cttgcatcgg catcatgaag ttcggctgca tttcattcgg accgactccg
     3421 tagaaacctt gaggagggca accacaacca cagccgccac agccaaaatg atgcatcggc
     3481 atgcaaggat ggccgaatgc atatggttgc tgctgcatca tcccgtaagg attttgaaat
     3541 tcaggggata ccatttgagg tacatacccc tgctcaggag ggaaatgatc catctgttcc
     3601 ggtgattcca tcggttccgt cggggactcc atcaattccc aacccctgat cggatatgga
     3661 ggggaaaccc ctggacccct tccagggtaa atcggcgatt cttcaaactc ttccagctct
     3721 tcggtcatgt ctatttcagg aggcttcact ccgagttgcg caggcggcat cggcatttcc
     3781 tgtgtaggca tgacttgttg cggcatgact ggtgccggca tttgttgagt gaagcttggg
     3841 aatgtcatcg gtgcgcagtc tgcatcataa atcggcatcc atccgcaagg taccggcatg
     3901 catggttgga aaaattgagg catctgcatt ggaggcatct gcatcggcgg catcgggacg
     3961 ggtggcggca ttggcggtaa agatggtaca gtcgacaggg tctgcatcgg tgattcggcc
     4021 ggcggcgtca ttgcagcagg cggcttctga acaggtgcac tcggaatagg cggcttcggc
     4081 atttcaaatt tttccttcgg caatggcata ggctccttcg gcttgccagg tttttcatgc
     4141 gcttgcggcg gagcctgagg ttgcttcact ttctccggca tcttttcctt gcctttccca
     4201 cttggcatat gctcggtctt cttcttaggc aagaaaatct tcatccccgg aacgatatag
     4261 tccgggttcg ccaaatgggc attcaccctt ttcaaatctt caaaagggat accgtactgt
     4321 cgtgcaattt tccacaacgt atcccctttt acaacaatat ggacatccac taacaaaatc
     4381 ccccttccat caatccatcg tatgaacgaa cgcccgtcaa tgttacgcaa atttaggaaa
     4441 ggcgccctct ctcttagtat gtatattgtg gaaaaggctt gattattcat tcgaaaaatt
     4501 gatgtactgc aagcttcatg cataaaaaaa ccttcacgcc gatattagcg tgaaggtctt
     4561 tgttcataac tgaaataaga gccgaaagat ctcacagcga cgttaagtgc ggcaagctcc
     4621 tcaagcgccc ctttcatcat tgggtgttct tcactttcca atacatcaat aatgaaaaaa
     4681 taatttccca aaccggtctt catcggcctg gattcgattt tgcttaaatt cagctttcgc
     4741 caagcaaaca ccgaaaggat ttgatggagc acccctgagc ggtcgtcctc aggcaacttc
     4801 accatgaagg tcgtttttgg caattccgaa tgaccctgct cttccaattg ccctttgcgc
     4861 attgacaaga cgacaaacct cgtatggttg aaatgaaagt catggatatt cttttccgcg
     4921 atatgtaatc cgtatttttc agcagccaaa ctgtttccga tcgccgctat gcaaagctcc
     4981 ggattttcgg aaatgtactt tgcagctgcc gcagtcgatg tcgtctggat caatggcgta
     5041 cggcggtaat gatattgcaa atatttatga cactgagcaa gcgctactgg atgggaatga
     5101 atcgatgcta attttcccga ttcatccttt cgtattttat tcaccatgag atgctgttca
     5161 attggagtcg atatttcggc
//
LOCUS       DASULI010000173         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_572568, whole
            genome shotgun sequence.
ACCESSION   DASULI010000173 DASULI010000000
VERSION     DASULI010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572568"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /gene="glmM"
                     /locus_tag="VFX34_03770"
     CDS             complement(<1..986)
                     /gene="glmM"
                     /locus_tag="VFX34_03770"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5564054.1"
                     /translation="MTKYFGTDGVRGIANKELTPELAFKLGRIGGHVLTKDSQETAQV
                     LVGRDTRISGHLLENALIAGLLSMGIEVMRLGIISTPGVAYLTRAMNAQAGVMISASH
                     NPFEDNGIKFFGADGFKLTDEQEDEIESLLHEETDQLPRPVGADVGTVTDYFEGGHKY
                     IQYLKQTVDEDFTDLHVALDCAHGATSSLATHVFADLDADLTTIGASPNGLNINDGVG
                     STHPEGLSQLVVDKGADIGLAFDGDGDRLIAVDELGRIIDGDQIMFIIGRHLQSKGRL
                     KSDTIVSTIMSNLGFYKALERHGMTSVKTAVGDRYVVEEMRKGNYNLGGEQSG"
     gene            complement(1027..2310)
                     /locus_tag="VFX34_03775"
     CDS             complement(1027..2310)
                     /locus_tag="VFX34_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX5564055.1"
                     /translation="MVDNLMDRPWFLRFTALALAIILFFSVQAENEKKMSSTIGDSHD
                     ILRDVPVEVLIDNENFVVTGVPKTVNMAIDGPASLVQSTKLLKDFTLKVDLRSKTMGK
                     HTVEIETENLSDKLDVRLEPATVDVVIEEKVTKTFPVEPEFNERLLAEGFNAVKKEVE
                     PATIQVTGAKSVIEAISFVKVSATGDPGINKSFEQKARVRVLDRDLNKLNVTIVPEEV
                     TVKVEVKENSKEVPVVLRQRGEPGENIVIDSVFTDTKTVTLFGPQKTLESIDELRVDI
                     DISKVKESGTVEMNLPKPTGVSKLSEEKIKVNISVTVVDGEEPPPDVSMEPTTDVEKD
                     VTKEFKNIPVAVKGLDEKYSSTFLKPANGVVDVTVTAKEEVIKSLDSSDFDISVDASG
                     TVQEGEHSYPLTVVGPDNIAWKLSENEVTLEIRLA"
     gene            complement(2303..3139)
                     /gene="cdaA"
                     /locus_tag="VFX34_03780"
     CDS             complement(2303..3139)
                     /gene="cdaA"
                     /locus_tag="VFX34_03780"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX5564056.1"
                     /translation="MPGWEMITNSSPIEILKNIVDVLLVWFVFYKLITIIKGTKAVQL
                     LKGIFVILLARILTEYFGLDTLKWMMDQVLMFGFLAIIILFQPEIRRALEQLGRGRLF
                     ARSAMQEEEERDRMIEAFTKSVSYMAKRRIGALISIERETGLSEYIETGTPLNSIVTS
                     ELLINIFIPNTPLHDGAVILQKNRIAAAGCYLPLSENPFISKELGTRHRAALGISEVT
                     DAITIVVSEETGAVSITANGDINRNLSMDEFEMQLRQIWFGAETETSSTPFWKWGKQK
                     NG"
     gene            complement(3342..3974)
                     /locus_tag="VFX34_03785"
     CDS             complement(3342..3974)
                     /locus_tag="VFX34_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5564057.1"
                     /translation="MNTCQEPVIHLMHAYLDGDISREDEQILQKHLAACDDCREVMDE
                     LKMSALFLKDAAPIQAPEGFVSGVMARLPKEKSQAGFQRLLRRHPLVAAAALFLILMS
                     ATLFSSYSNNQQFSFTKQPNLVVEGETVVVPAGEVVKGDIVVKNGNLRIEGEVDGNVT
                     VIHGTKYMASTAVVTGKSEEIDQAFDWLWYKIKSTVKDIFPSSDEEPQAE"
     gene            complement(3988..4551)
                     /gene="sigW"
                     /locus_tag="VFX34_03790"
     CDS             complement(3988..4551)
                     /gene="sigW"
                     /locus_tag="VFX34_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigW"
                     /protein_id="HEX5564058.1"
                     /translation="MDELVNKRINQVIKGDQDAFEEIVTMFQHRLYHVCYRMLGNPQE
                     AEDIAQEAFVRAYVNIHTYDQKRKFSTWLFRIATNLCIDRIRKKKPDYYLDATVPGTD
                     GLNMYSQIAAAGDLPEEEVERMEAQERVQYEIGRLPEKYRTVIILRYMEELPLQEISD
                     ILELPLGTVKTRVHRGREALRKQMGTM"
     gene            complement(4723..>5102)
                     /locus_tag="VFX34_03795"
     CDS             complement(4723..>5102)
                     /locus_tag="VFX34_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX5564059.1"
                     /translation="SVDPGERILIKDLGMKVFTMHEIDRDGMTSVMKEAIAYLQAKNV
                     DGVHLSLDLDGLDPLYTPGVGTPVPGGISYRESHLAMEMLEDSGMITSAEFVEVNPIL
                     DEKNKTADVAVGLMGSLFGEKLL"
BASE COUNT         1231 a         1369 c         1030 g         1472 t
ORIGIN      
        1 ccggattgct ccccgccaag attgtaattc ccttttctca tctcttctac tacgtaacgg
       61 tcgccgacag cagttttgac actcgtcatc ccatggcgct ccaatgcctt gtagaagccg
      121 aggttgctca tgatcgtgga aacaatcgtg tcggatttca gacggccctt cgattggagg
      181 tggcggccga tgatgaacat gatctggtcc ccatcaatga tccgtcctaa ttcgtctacg
      241 gcaatgagac ggtctccgtc cccgtcaaat gcaagtccga tatccgcacc tttatctacc
      301 actagctggc tgagaccttc cggatgagtc gacccgacac catcattgat gttcaagcca
      361 ttcggagacg cgccgatggt cgtcaagtca gcatccagat cagcaaagac atgcgttgcc
      421 aatgatgaag ttgcaccatg cgcacagtca agggcgacat gaagatcagt gaaatcttcg
      481 tccactgtct gtttcaagta ttggatgtat ttatggccgc cttcaaagta atccgtcact
      541 gtgccgacat ccgcacctac cggacgaggc agttgatccg tctcttcatg taagagggat
      601 tcaatttcat cttcctgctc atccgtcaat ttgaacccgt ctgcaccgaa aaacttgatg
      661 ccgttatctt caaatggatt gtgggatgct gaaatcatga cacctgcctg tgcattcatg
      721 gcgcgcgtca aatatgcgac acctggtgtg ctgatgattc caagcctcat cacttcgatt
      781 cccatcgaca aaaggcccgc aattaaagca ttctccagca aatggccgga aatgcgtgta
      841 tctcgtccaa caaggacttg tgccgtctcc tgggaatctt tcgttaatac atgcccgccg
      901 attctgccaa gtttaaatgc aagctccggc gttaattctt tatttgctat cccacggacg
      961 ccatccgttc cgaaatactt tgtcataatc catactccct ttcaatgctc ttaatcagct
     1021 tcttcatcat gcaagcctaa tttccaatgt cacttcattc tctgaaagct tccaagcaat
     1081 attatcagga cctactacag tcagcggata tgaatgctcc ccttcctgaa ccgtcccgga
     1141 agcatcgacg ctaatatcga aatccgaact gtcgagtgat ttgatgactt cctctttggc
     1201 agtcaccgtc acatcgacaa ctccattcgc aggtttcaaa aaggtgctgc tatatttctc
     1261 atccagccct ttcacagcga cagggatgtt tttaaattcc tttgttacat ccttctccac
     1321 atcagtcgtt ggttccatcg atacatcagg cggcggctcc tctccatcta cgaccgtcac
     1381 gctgatgttc actttgatct tctcctccga aagcttggaa actcccgttg gctttggaag
     1441 gttcatttct acggttcccg attccttcac tttcgagata tcgatatcca cccgaagttc
     1501 gtcaatggac tcgagggtct tctgcgggcc gaacagcgtt actgttttcg tatctgtgaa
     1561 aacagagtcg atgacgatgt tctcgcccgg ctctcctctt tgtctaagga caactggaac
     1621 ctctttgcta ttctctttca cttccacttt gactgtcact tcttcaggta cgatcgtgac
     1681 attcaattta ttcagatccc gatcgagcac ccggacacgc gctttttgtt caaatgattt
     1741 attgatgccc ggatctccgg ttgccgaaac cttcacgaag ctaattgcct cgatcacgct
     1801 tttcgcacca gtcacttgaa tggtcgcagg ctcgacttcc ttcttcacgg cattaaaccc
     1861 ttctgcaagc agccgctcat taaattcagg ctcgactggg aacgtttttg ttactttctc
     1921 ttcgatgacg acatccacag tggccggttc caatctgaca tccagcttat ccgataggtt
     1981 ttctgtttcg atctcgacag tatgctttcc cattgtcttg ctcctaaggt ctaccttcaa
     2041 tgtaaaatcc ttcaacagct ttgtagactg gacgagactg gcaggcccgt cgattgccat
     2101 attgaccgtc tttgggactc cggttacaac gaaattttca ttatcaatca gaacttccac
     2161 aggcacatcg cgaaggatat catgtgaatc accgatggtg gaactcatct ttttttcatt
     2221 ctccgcctgg acggaaaaga ataggatgat cgcaagcgcc aatgccgtga aacggagaaa
     2281 ccatgggcga tccattaaat tatcaaccat ttttttgttt cccccatttc cagaatggag
     2341 tggaagacgt ttcggtttcc gccccaaacc atatttgacg aagctgcatc tcaaactcat
     2401 ccatcgatag attccgattg atgtccccgt tagcggtgat actgacggcg cctgtctctt
     2461 cggaaacgac aattgtgatg gcatcggtca cttcactgat gccgagagcc gcccgatgcc
     2521 gggttccaag ttccttggaa atgaaaggat tttcggagag cggcaaatag caacctgcag
     2581 ccgcaatgcg attcttttgc aaaatgaccg ccccgtcatg caacggcgta ttcggaatga
     2641 agatattgat aagcagttcc gaagtgacaa ttgagttcaa cggggttccg gtttctatgt
     2701 actcacttaa gccggtctcc ctttcaatcg aaataagcgc gccaatccgc cgcttcgcca
     2761 tatagcttac cgacttggtg aacgcttcaa tcatacgatc acgttcctct tcttcctgca
     2821 ttgcagatct cgcaaacaaa cggcctcttc caagttgctc caacgcacgg cgtatttcgg
     2881 gttgaaaaag gataataata gctaggaaac cgaacatcaa cacttgatcc atcatccact
     2941 tcaatgtatc aagcccgaaa tattcagtca ggatacgggc caataaaata acgaaaatcc
     3001 cttttaacag ctgtactgcc ttcgtgcctt ttatgatggt gatcaattta tagaaaacaa
     3061 accaaacgag aagcacatcc actatatttt tcagtatttc aatcggactt gagtttgtta
     3121 tcatttccca accaggcatg cgcttccctc accttcatcg caaattcttt acttcagtat
     3181 atcatacagc cttgtttcaa tgctcttgaa aacaaacagg accggttacg aatgggaacg
     3241 tttttcctga tgttttgttc cctttcctgt atgtctatag tttgttcatt taaattaaaa
     3301 acgaccgctc tcccgtagcc gggaaagcgg tcgcaatcat cctattctgc ttgtggctcc
     3361 tcgtcggagg aagggaagat gtccttgacg gtcgacttga tcttatacca tagccagtcg
     3421 aaagcttgat caatctcttc actttttcct gtaacgacag ctgtggaagc catgtatttc
     3481 gttccgtgga taaccgtcac gttgccatcc acttcccctt cgatccggag attgccgttt
     3541 ttaacgacga tgtccccttt cacgacttcc ccggctggaa ctacaaccgt ctccccttcg
     3601 acgacgaggt tcggctgctt cgtgaaggaa aactgctgat tattgctata acttgaaaag
     3661 agcgtcgcgc tcattaaaat taaaaatagt gcggccgcag ccacaagtgg gtgcctgcgt
     3721 aatagtcgct gaaagcctgc ttgcgatttt tctttcggga gacgtgccat gacgcccgag
     3781 acaaatcctt caggtgcttg gatcggtgca gcatccttca ggaataaagc tgacattttc
     3841 aactcatcca tcacttccct gcaatcatca caggcagcta aatgcttttg cagtatctgc
     3901 tcatcttcgc ggcttatgtc tccgtctaaa tacgcgtgca ttaaatggat aactggttcc
     3961 tgacacgtat tcatacttta ttccctccta catagtaccc atctgtttcc gtagcgcttc
     4021 ccgtccacgg tgaacccggg tttttacggt gccaagtggc aactccaaaa tatcactgat
     4081 ctcctgcaaa ggcaattctt ccatatagcg cagaatgatg acagtgcggt atttttcagg
     4141 cagccgtcca atctcatact gaacacgttc ctgcgcttcc atacgctcca cttcttcctc
     4201 cggcaagtcg cctgccgctg ctatctgcga gtacatgttc aggccgtccg ttccaggcac
     4261 tgttgcatcg agataataat ccggcttctt tttgcggatc ctgtcgatgc ataaatttgt
     4321 tgcaatccga aatagccaag tggagaactt ccttttctga tcatacgtat ggatattcac
     4381 atatgcacgt acaaaagctt cctgggctat atcttccgct tcctggggat tgccgagcat
     4441 tcgataacaa acatgataca gccgatgttg aaacatggtg acaatttctt cgaatgcatc
     4501 ttgatcgcct ttgatcactt gatttattct tttattgacc aattcatcca atgactttca
     4561 tcctccgctc tatgcggctg ttccttctat acgattgaag ggttcaacag gtttcatttt
     4621 ttaatccata ttttttattt taccaaagat tgataattat ttccgttgtt ttggaaaatt
     4681 catttgcaaa aaacgcctgg ataggaatcc aagcgcccaa tcttataata gcttttctcc
     4741 gaacaatgat cccatcagtc cgactgcgac atccgcagtt ttgttctttt catccaggat
     4801 cggattgacc tcgacgaact ctgcagatgt gatcatgcct gaatcctcaa gcatctccat
     4861 tgcaagatgg ctttcgcggt agctgattcc gcccggcacc ggcgttccga ctccaggggt
     4921 gtacaacgga tcgaggccgt ccaaatccaa ggataggtgg acgccatcca cattctttgc
     4981 ttgtaaataa gcaatcgctt ccttcatgac cgaagtcatg ccgtcccgat caatttcatg
     5041 catcgtaaac actttcattc ctagatcttt tatcaatata cgttcgcccg gatcgactga
     5101 ac
//
LOCUS       DASULI010000174         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_559577, whole
            genome shotgun sequence.
ACCESSION   DASULI010000174 DASULI010000000
VERSION     DASULI010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559577"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFX34_03800"
     CDS             <1..819
                     /locus_tag="VFX34_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016716868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5564060.1"
                     /translation="ELGVQGAAISSVGSRVIAMFVFIWLLYQVLEYRIEFKYYITWSK
                     EYVKKILNIGLPSAFEQILYQACQIIFLYYVTYLGTEALAARQYATNISMFSYLFALA
                     IGLGTSIIVGRLVGSGEKDEVYRQVWASLKRALVFTVVLVSLVMTFRYPLMRLFTENE
                     QIIELGASVLLLSCLLETGRTFNMVIINSLRASGDAKFPVLMGAISMVMMSLPLGYFF
                     VFVLDLGLVGVWLAVIMDEWVRAIVMSLRWKSRAWERYALVEPLPEVEAKPEVV"
     gene            complement(886..1176)
                     /locus_tag="VFX34_03805"
     CDS             complement(886..1176)
                     /locus_tag="VFX34_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019720450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564061.1"
                     /translation="MSVQNYHALCNRHIGRAVVIGTRDGRTHRGIIQGVRGNRVFLEP
                     FGRDRNFGGFGYGFRGGFGFGRGFGFGRGFAFGAGIALGSIVTFALLPFIFF"
     gene            complement(1297..2073)
                     /locus_tag="VFX34_03810"
     CDS             complement(1297..2073)
                     /locus_tag="VFX34_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HEX5564062.1"
                     /translation="MAVVRTDNWLEECRGDPSKVCEKLSEHFPTATAAEIHRHLTMFG
                     MYLSAKQGRELSKKLYDKNVWEISSNEMKQLQRKWNGPSVPVFIFPSDIDNKVLVREF
                     NGKSGLAYADKIFLFISPQNTETEIKAILTHEYNHVCRLSKFPKREDDCTLLDTIILE
                     GLAEMAVTERFGKDAASQWTSLYSDQELDRLWKKIVYPNRHHVKDSRIHEKVLYGLGN
                     YPRMAGYAVGYYLVRRFVKESDLDAEDLFTLPSTDIAQLS"
     gene            2214..>2660
                     /locus_tag="VFX34_03815"
     CDS             2214..>2660
                     /locus_tag="VFX34_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5564063.1"
                     /translation="MRVVNNIAELIGEAPLVRLNRLPDQRGADVYVKLEYFNPSRSVK
                     DRAAYNMIIEAEKAGYLQPGDTIIEPTSGNTGIGIAMNAAARGYKTIIVMPDSSTLER
                     INILKAYGATVVLTPSDLKMPGAIQKAEEIAAGIPNSFIPMQFENVA"
BASE COUNT          698 a          631 c          645 g          686 t
ORIGIN      
        1 gagcttggtg tgcagggggc ggcgatatct tcggtcggca gccgggtgat cgcaatgttc
       61 gtcttcattt ggcttctgta ccaggtactt gaatatcgca ttgaatttaa gtactacatt
      121 acgtggtcaa aggaatacgt caaaaagatt ttaaatatcg gtctgccctc agcgttcgag
      181 cagatcctct accaggcgtg ccagatcatc ttcctctact acgtcaccta cttggggacg
      241 gaggcgctcg ccgcgagaca atacgcgacg aacatatcga tgtttagcta tttgtttgca
      301 ctggcgatag gacttggaac gtcgatcatt gtcggacgtc ttgtcggaag cggcgaaaag
      361 gatgaagtat accggcaagt ttgggcgtca ttgaaacggg cgctcgtctt tacggtcgta
      421 ttggtgtcgc ttgtcatgac gttccgttac ccgctcatgc ggctcttcac tgaaaacgag
      481 cagatcatcg agctcggtgc atccgtcctg ctattgtctt gtttgctgga aaccggcagg
      541 acgttcaata tggtcatcat caactcgctc cgtgcatcgg gcgacgcaaa attccctgta
      601 ttgatggggg ccatttcaat ggtcatgatg agccttccgc tcggctactt ctttgtgttc
      661 gtcctcgatc tcgggcttgt cggtgtttgg ctcgccgtca tcatggacga atgggtgcgc
      721 gcaatcgtca tgtcgctccg ctggaaaagc agagcttggg aaagatatgc gcttgtggaa
      781 ccattgcctg aagtggaagc gaagccggaa gttgtgtaat taagaaataa aaagaagcag
      841 gccgctcact agatcgatag tgtgcggcct gctttattat gatcatcaaa agaagataaa
      901 ggggagtaac gcaaaggtta cgattgagcc aagtgcaata cctgcaccga aagcaaaccc
      961 gcgaccaaag ccgaatccgc gaccaaagcc aaatccgcct ctgaatccgt atccgaatcc
     1021 gccaaaattt ctatcgcggc cgaaaggttc caagaaaacc ctattgcctc tcacgccttg
     1081 aatgatccct ctatgtgttc gtccatccct cgttccgatg acgacggccc ttccgatatg
     1141 cctattgcac agagcatgat agttttgaac tgacatcgtg ttgtcacccc atttcgattg
     1201 aacataatac atcatatgtg agggattttt cttcaaggag gacaaacaca ccaatgcaat
     1261 tgtttatagg tgcccttatc tgcacaccct aaccgattag gatagctggg caatatcagt
     1321 tgaagggagt gtgaataaat cctcggcatc caagtctgat tcttttacaa acctgcggac
     1381 taaataatag ccgacggcgt atccagccat acgcggataa ttcccgagac catacagcac
     1441 tttctcatgg atgcggctat cctttacgtg atgccgattc ggatagacaa ttttcttcca
     1501 taacctatcc aactcttggt cagaataaag agatgtccat tgggaggcgg catctttgcc
     1561 gaatctttcc gtgacagcca tttctgccaa cccctccagg atgatcgtat ctaacagcgt
     1621 acagtcgtct tcccgcttgg gaaacttgga cagccggcat acatggttgt actcatgggt
     1681 caatatcgcc ttgatttcag tttccgtatt ttggggcgaa ataaataaaa aaatcttatc
     1741 tgcataagct aaccccgact ttccattgaa ttctcgaacc aacactttat tatctatatc
     1801 tgaagggaag atgaagacgg ggacggaagg accattccat ttacgttgca attgcttcat
     1861 ctcatttgat gagatttccc atacgttttt gtcgtataac ttcttgctga gctcccttcc
     1921 ttgttttgca ctcaaataca tgccgaacat cgttaagtga cgatgaattt cagcagcggt
     1981 agcagtaggg aagtgttcgg atagtttctc gcaaactttg gaagggtctc cgcggcattc
     2041 ctctagccaa ttgtctgtcc ggacaacagc catgtggtga cacccctttt tgaattagca
     2101 tattccggag atgcggggaa ggtccgagaa ataaaaccta ctaaattact atacataata
     2161 ggatttaatc tgctataatc aactcaacca tgaaacacaa ggagctgaac ggcttgcgag
     2221 tagtgaataa tatagccgaa ttgatcggcg aagcaccttt agtgagattg aatcgccttc
     2281 ccgatcagcg gggcgcagac gtgtatgtga aacttgaata tttcaatccg agccgaagcg
     2341 tgaaagaccg ggcggcatac aatatgataa tcgaagcgga aaaagcgggt tacctgcaac
     2401 cgggggatac gatcatcgaa ccgacatccg ggaatacggg aatcggcatc gcgatgaacg
     2461 cagctgcgag agggtataaa acaatcatcg tcatgccgga ctcctcgacg ctcgagcgga
     2521 tcaatatttt aaaagcatac ggtgcaacgg ttgtattgac gccaagtgat ttgaagatgc
     2581 cgggcgccat tcaaaaggcc gaggaaattg cggcgggaat tccgaacagc ttcattccga
     2641 tgcaatttga aaatgtggcc
//
LOCUS       DASULI010000175         4784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_65203, whole
            genome shotgun sequence.
ACCESSION   DASULI010000175 DASULI010000000
VERSION     DASULI010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65203"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFX34_03820"
     CDS             <1..315
                     /locus_tag="VFX34_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HEX5564064.1"
                     /translation="DNIGEEILGKQPDAIFKDAAASSFNNLRTELLMKKADLVIALFG
                     EQYKQWNTAMDASAAVAYGKPLILIRSEQHHHPLKELSRKAHATVETVDQAVKALHYI
                     FE"
     gene            683..1501
                     /gene="pstB"
                     /locus_tag="VFX34_03825"
     CDS             683..1501
                     /gene="pstB"
                     /locus_tag="VFX34_03825"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019376268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5564065.1"
                     /translation="MTLLLDRIKEKPLSVVRNESWHKPIYETNGLNLWYGTSHALKNI
                     DLSINEREVTAIIGPSGCGKSTFLKTLNRMVETAADVSISGNVTFKGNNILGKAMPVE
                     ILRSKVGMVFQKPNPFPKSIYENVAFGPKVHGIRNKATLDMIVEESLRKAALWDEVKD
                     RLHKSAYGLSGGQQQRLCIARCLAIDPEVILMDEPTSALDPVSTHKIEELISSIKNDV
                     TIAIVTHNMQQAARISDRTAFFLNGEVIEEDRTTTIFNNPSDTLTDDYINGRFG"
     gene            1671..2636
                     /locus_tag="VFX34_03830"
     CDS             1671..2636
                     /locus_tag="VFX34_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5564066.1"
                     /translation="MKFKKSLLFMIIAAFAVVLAACGKDGDSADKNNASAKGAELEGS
                     VAIDGSGTVYPLMARLAEEYMVNEQENVSVEVSRAGTSAGFKKFLVENGTDFNDASRQ
                     IKDEEAAGADKLGIEVKELKVALDGLTFVINKDNDWATEMTPEQLISIFLADSTVEKW
                     SDINPDWPDEKINAMGPNENHGTYEFFYESILDKQDMDSSVNLQQDYSTLVNLVSEDK
                     NGIAFFGFGYYVNNQDKLNAVAVDFGNGPVEPALDTIAEDGAYADFTRPVFTYLNVNH
                     AKEKPQVKDFAIYVMNNINKFAGETGFAPIPETEVKENVSFLEGL"
     gene            2823..3776
                     /gene="pstC"
                     /locus_tag="VFX34_03835"
     CDS             2823..3776
                     /gene="pstC"
                     /locus_tag="VFX34_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5564067.1"
                     /translation="MLNQELPKAEKTNIRDMIDQNQRGNSIRRRIERLIPIFLTLIAS
                     LSVLTTIGIIWTLLSETVEFFKRVPFLDFFTGTVLKPLSQNPEFGVLPLLMGTVTSSL
                     IAMAVAVPIGLMAAIYLSEYASPKVRKIVKPVLELLAGVPTIVYGFFAFTFVTPLLRE
                     FIPSLQATNILSPGLVMGVMIIPMIASLSEDAMSSVPNAMREGAYGLGATKLEVTRKV
                     VIPAALSGIIASFVLGISRAIGETMIVTIASGSTKNFTFDITQSMQTMTAYIVEVTGG
                     DAAAGSTVYYSLYAVAMTLFVFTLVMNLIARAVSKKFREEY"
     gene            3778..4662
                     /gene="pstA"
                     /locus_tag="VFX34_03840"
     CDS             3778..4662
                     /gene="pstA"
                     /locus_tag="VFX34_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010678128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5564068.1"
                     /translation="MNQLSEKQHVRRTKRRLFLNRFSKSIFMLSAFFGLFFLVVLLFK
                     VVSDGIGSINFDFLTGKLSTSPERAGIMGAILGTFWLMLVVAPVTMFLGVGTAIYLEM
                     YAKKGRLVSFIQTNISNLAGVPSIVYGILGLTVFVRALDLGNIVLAGGLTMSLLILPI
                     VIVASQEAIRSIPFYLSEASYGLGATKWQTIQRIILPAALPGILTGAILSLSRAIGET
                     APLVVIGIPALLIPFPGGIMDRFTVLPMQIYYWTLDASLVAEYANLAAATIIVLLVAL
                     FVLNSTAIIIRNKFQRVF"
BASE COUNT         1292 a         1148 c         1122 g         1222 t
ORIGIN      
        1 gataatatcg gtgaggagat tttaggcaag cagccggatg caattttcaa ggatgctgcc
       61 gcttccagtt tcaataactt gcggactgaa ctcctcatga aaaaagcgga tcttgtcatt
      121 gcccttttcg gcgagcagta caagcaatgg aacacggcga tggacgcgag tgccgcggtt
      181 gcatacggca agccgcttat cctcatccgt tccgagcagc atcaccatcc tctgaaggag
      241 ctttcccgca aagcgcatgc tacggtcgag acagtggacc aagccgtgaa ggcattgcat
      301 tatatttttg aatgaacgca aaggctatcc aaacgtggat ggccttttta tatttggata
      361 tctttgcgat ttatcaccac tcggaacgat gttatcaccg cttacattga ctttatcacc
      421 gcttagagga actttatcac cactcagaac acctttatca ccacttatcg agattttccg
      481 taactatacc accgattcgg gcgggtttac caccgactct cgccacttta ccaccgattc
      541 ggcaaatttc agtctaatac cgaacagttt ccaggtagaa cccttcactt ctctttacaa
      601 aacctttaca cttcgttcat actccctcaa taatcctccc gtacactaaa cgtaagcctc
      661 aaaacaacta ggaggattcc aaatgacatt actacttgat cgtataaaag aaaaaccatt
      721 atcagtcgtg cgaaacgaaa gctggcacaa gccgatttat gaaacgaacg gcttgaatct
      781 ctggtacggc acgtcgcatg cgttaaagaa catcgacctc tcaataaatg aacgcgaagt
      841 gaccgccatc atcggtccat cgggctgcgg aaaatcgaca ttcctgaaaa cattgaaccg
      901 gatggtggaa acagcagctg acgtctccat ctcaggcaat gtcacgttca aagggaataa
      961 tattttgggc aaggcgatgc ctgtcgaaat tcttcgttcc aaagtcggca tggtgttcca
     1021 aaagccgaac cccttcccga aatcgattta cgaaaacgtc gcattcggac cgaaagtgca
     1081 cggcatccgt aacaaggcga cactcgacat gattgtggaa gaaagcttga gaaaagcggc
     1141 attatgggat gaagtgaaag accggcttca taaaagtgcg tacggccttt ccggcggaca
     1201 acagcagcgt ctatgtattg cccgctgcct cgccatcgat ccggaagtga ttttaatgga
     1261 cgaaccgaca tccgcacttg acccggtttc aacgcataag atcgaggaat tgatcagctc
     1321 cattaaaaac gatgtgacga ttgccatcgt tacgcataat atgcagcaag ccgcacggat
     1381 ttccgatcgt accgccttct tcctgaacgg cgaagtcatc gaagaagatc ggacgactac
     1441 aatcttcaac aatccttcag atacattgac agacgactat attaacggcc gttttggatg
     1501 aatcaaaaac ccgctcaact tagagcgggt ttttaattta ttcattcaac actaatttac
     1561 aataccttta cacattaaca atactcactt aacaataacc ctttaatatg tagttgtgag
     1621 ggagattcct tcatctatat cagaacataa acccagggga ggatttatca atgaagttca
     1681 aaaaatctct tttattcatg atcatcgctg cgtttgcagt tgttcttgca gcatgtggca
     1741 aagatggcga ctccgcggac aaaaacaatg catctgcgaa aggtgcggaa ctggaaggca
     1801 gtgtcgctat tgacggttca ggaactgtgt acccgcttat ggcacgtcta gctgaggagt
     1861 atatggtgaa cgaacaagag aacgtatccg tcgaagtgag ccgtgcaggt acaagtgctg
     1921 gcttcaagaa attcctcgtt gaaaacggaa cggacttcaa cgatgcttcc cgccaaatta
     1981 aagacgaaga agcagccgga gcagataagc ttggcatcga agtgaaagaa ttgaaagttg
     2041 ccctggacgg ccttactttc gtcatcaata aagataatga ttgggctact gaaatgacgc
     2101 ccgaacaatt gatcagcatt ttccttgcag acagcacggt cgaaaaatgg tccgacatca
     2161 atcctgattg gccggatgaa aagatcaatg cgatgggacc gaatgaaaac cacggtactt
     2221 acgagttctt ctatgaaagt atcttagata agcaagatat ggatagctca gtgaacttgc
     2281 aacaggatta ttcaacactt gtaaaccttg tttctgaaga taagaacggc atcgcgttct
     2341 tcggattcgg ctactacgtc aataaccaag acaagttgaa cgctgtcgcg gtcgacttcg
     2401 gaaacggacc tgtcgagccg gcactagata cgatcgctga agatggagcc tatgcagact
     2461 tcacacgtcc ggtcttcaca tacttgaatg tgaaccatgc aaaagaaaag ccacaagtga
     2521 aagactttgc aatttacgtc atgaacaata ttaataaatt cgcaggcgaa acaggattcg
     2581 caccgattcc ggaaactgaa gtgaaagaaa acgtcagctt cctcgaaggc ctgtaataag
     2641 caacaaaagc gtaagcgcct gttcaggggc gacaggcata agacggattg gcgaagaggc
     2701 ggtttttgcc tcaaagccaa tatggcttat gacccgagcc cctaggcgct ggagtctaga
     2761 cgaggattgg atggaggcaa aagccttcat cctcttgcat cttactggaa ggggtatcat
     2821 taatgctaaa tcaagaacta cctaaggcag aaaaaacgaa tatccgtgac atgatcgatc
     2881 aaaaccaacg tggcaattcg atcagaagaa gaattgaacg gctcattccc atcttcctca
     2941 cattgattgc ctccttatct gtccttacga caatcggcat catttggaca ctgctatccg
     3001 aaacggtgga gtttttcaaa cgggtgccgt tcctcgattt ctttacgggc accgtcttga
     3061 agccattgag ccagaatccc gaattcgggg tgcttccatt attgatgggt accgtcactt
     3121 cttctttgat tgcgatggcg gtagccgtgc cgatcgggtt gatggcagcc atttacttga
     3181 gtgaatacgc ttccccgaaa gtacggaaga tcgtgaaacc cgtgcttgag ctgctggcgg
     3241 gcgtgccgac aatcgtctat ggatttttcg cattcacttt tgtcacaccg ctgttgcgtg
     3301 aattcattcc aagccttcaa gcgacgaata ttcttagccc cggcttagtg atgggtgtca
     3361 tgatcatccc gatgatcgct tccctatccg aggacgcgat gagctccgtg ccgaacgcaa
     3421 tgcgtgaagg cgcgtatgga ttaggagcaa caaaactaga ggtgacacgt aaggttgtca
     3481 ttcccgcggc cttatccggc atcatcgctt cgttcgtgct aggaatatcc cgtgcgatcg
     3541 gggaaacgat gattgtgacg atagcgagcg gcagtacgaa aaacttcacg ttcgatatta
     3601 cacaatcgat gcagacgatg accgcctata tcgtcgaagt gacaggcgga gacgccgcag
     3661 ccggctcgac agtctattac agcttgtatg cagtggctat gactcttttt gtattcacac
     3721 ttgtgatgaa cctgattgca agagctgttt ccaaaaagtt cagggaggaa tattaagatg
     3781 aatcaactga gcgaaaaaca acatgtacgg cggacgaagc gccgtttatt cctgaaccgg
     3841 ttctccaagt ccattttcat gctatctgcg ttcttcggac tgtttttcct tgtcgtattg
     3901 cttttcaaag tcgtatcaga tggaatcggt tcaatcaact tcgatttctt gacaggtaaa
     3961 ttatcgacgt cgccagagcg cgcaggcatc atgggcgcga ttctcggaac gttttggctt
     4021 atgctcgttg tcgctcctgt aacgatgttc ttaggagtcg gaacggccat ttacttggag
     4081 atgtatgcaa agaaaggccg cttggtcagt ttcattcaaa cgaatatctc caatttagca
     4141 ggcgtccctt ccattgtgta tgggattctg ggcttgacgg tattcgttcg agcgctggac
     4201 cttgggaata tcgtccttgc cggcggattg acgatgtcat tattaatctt gccgattgtc
     4261 attgtcgcaa gccaggaagc gatccgctcc atccctttct atttgagcga ggcatcttac
     4321 ggattgggtg caacgaaatg gcaaacgatt caacggatca tcttgccagc agctcttccg
     4381 ggaatcttga cgggtgcgat tctatccttg tcccgtgcca tcggggaaac cgcaccgctc
     4441 gtcgtcatcg gaatcccggc attgcttatt ccgttccccg ggggcatcat ggaccgtttt
     4501 acggtgttgc ctatgcagat ttattactgg acgctggatg cttcgttggt cgcggaatat
     4561 gccaatctgg cagccgccac gattattgta ttgctagtgg cgttgttcgt tctaaattcc
     4621 acggcgatca ttatccggaa taaattccag cgtgtatttt aagctccatc gcatccaatt
     4681 gaggatgcga tttttttatt cgtttcgtta ctggtcttgt gccgcctacc gatccatgca
     4741 aaccgcctaa agatggcccg tgaccgccta aacgagacgt ccga
//
LOCUS       DASULI010000176         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1070539,
            whole genome shotgun sequence.
ACCESSION   DASULI010000176 DASULI010000000
VERSION     DASULI010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070539"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VFX34_03845"
     CDS             complement(<1..750)
                     /locus_tag="VFX34_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564069.1"
                     /translation="MEKLSTEQYLQATDFLKSKARPLEKALFEFEFENGSREQVLEEL
                     EVYQNDDGGFGNGLEPDFRCEASSALATTIGLQHLIRIGADESEPMVQRAIQYALDTF
                     DDEKMGWEIVPKEVETAPRAIWWNYGGDWPWGNPSAEMTGLLHHYKGLVPEDFLEKLT
                     AHAVRYINEEATNDFHELQCLVKLIRELPAEEAEKIAVRVREIARHSVTTKPDKWHDY
                     CLLPLQVVSSPSSFLYEDFEEIIPANVGHLAS"
     gene            complement(870..2405)
                     /gene="dacB"
                     /locus_tag="VFX34_03850"
     CDS             complement(870..2405)
                     /gene="dacB"
                     /locus_tag="VFX34_03850"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010648072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX5564070.1"
                     /translation="MSILMKKQCLLKRAFLAMAIALIALFPVAHEGTLSSVAASGDTT
                     PAASIDSTTLDQKLQAILKDPKLQGGITGVSIRKADTGEKIYSHYGDIRLRPASNMKL
                     LTGSTAMDILGPDYQFSTEVLTDGEVKGKMIHGNLYLKGKGDPTLMKKDLDQFAKDLK
                     AKGIDKINGNLIADDSWYDDDRYSQDLNWSDEHNYVGAQVSALTLSPNEDYDAGTVIV
                     EVNAGDKAGDLPKVTLTPETDYVEIVNRATTVAKGEAKSISIAREHGTNRIIIEGKIP
                     VEGTRSQSWSAVWEPTGLTLDVFKKSLAEQGIEFVGNGGMETGIAPGNATVLVSKKSM
                     PLNELFIPFMKLSNNGHAETLVKEMGRVQRGQGSWSAGLAVMKEKLKQFGVNTDTVVL
                     RDGSGMSHKNLVSADELTTLLYSIQGKSWFPDFEASLPIAGVPERMVGGTLRNRMGSG
                     LTARNVTAKTGSITGVSTLSGYVTAKDGTELVFSIMINNYVTGPVTPIEDAIAKVLAE
                     YEF"
     gene            2635..3339
                     /gene="pxpB"
                     /locus_tag="VFX34_03855"
     CDS             2635..3339
                     /gene="pxpB"
                     /locus_tag="VFX34_03855"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003351021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX5564071.1"
                     /translation="MQYTLSQLGDQAVVIEVGEKIDASSERQVRTIVSLLEQKSPPWM
                     IEYIPAYTTVTLIYSIREFLKEDSPYEAVCARIRELLKDVKETAAVQQKVVEIPVLYG
                     GEFGPDLDFVAEHNGLTPDEVIAIHTSGEYMVHMIGFAPGFPFIRGMSEQIAAPRRKT
                     PRPKIPARSVGIAGLQTGVYPIETPGGWQLIGRTPLDLFLPEEDPPSLLSAGNKIRFY
                     KITSEEYKDMRGGAGD"
     gene            3332..>3506
                     /locus_tag="VFX34_03860"
     CDS             3332..>3506
                     /locus_tag="VFX34_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009334499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KipI antagonist"
                     /protein_id="HEX5564072.1"
                     /translation="MIEVTKPGLHDTVQDVGRFGYQKFGVVAGGAMDPFSHRVANMLV
                     GNNETEATLEMTLL"
BASE COUNT          873 a          829 c          819 g          985 t
ORIGIN      
        1 ggaagcaaga tgcccgacgt tagcagggat gatttcctca aagtcttcat acagaaaaga
       61 cgatggggag gacacgactt gaagagggag caggcaatag tcatgccatt tatccggttt
      121 tgtcgttacg ctgtggcggg caatctcgcg aactctaacg gcaatcttct ctgcttcttc
      181 cgcaggcaat tcccttatca atttcaccaa gcactgcaat tcatggaagt cgttcgtcgc
      241 ctcttcgttg atatacctaa cagcgtgggc tgtcagcttt tcgagaaaat cttcaggaac
      301 cagccctttg taatgatgaa gcaaccccgt catttcagcg cttggatttc cccatggcca
      361 atcaccgccg taattccacc agatggcgcg cggcgctgtt tccacttcct tcgggacaat
      421 ttcccatccc attttctcat cgtcaaaggt atcaagtgca tattgaatag ctcgttgcac
      481 catcggctcc gattcatccg ccccaattcg aatcaaatgc tgaagcccaa ttgtcgtcgc
      541 taacgctgaa gatgcctcac acctgaaatc gggctccaat ccatttccga aaccgccatc
      601 gtcattttga taaacctcca attcctccaa cacttgctcg cggctgccat tttcaaattc
      661 aaattcgaat agcgcttttt ccaatgggcg tgcttttgat ttgagaaaat ctgtagcttg
      721 aagatattgt tcagtcgata atttctccaa atgaatcccc actttcgaga gaatgattga
      781 tatggatact ttcgctattg ttatctttaa atccttccca aataaaaata aagacaccca
      841 ttaaatggat gcctttattt gatagtcaat taaaactcat attccgcaag cactttcgca
      901 attgcatctt caatcggcgt gacaggtcca gtgacgtagt tgttgatcat gatcgagaag
      961 accagttctg tgccgtcttt cgcggtgacg tatccggaaa gggtggagac gcctgtgatg
     1021 gagccggttt tcgctgtaac atttctggcc gtcaatccgc tgcccatccg gttgcggagc
     1081 gtgccgccga ccatccgttc agggacgcct gcaattggga gggaagcttc gaaatcaggg
     1141 aaccagcttt ttccttgaat gctgtacagc aaagtcgtta attcatctgc tgaaacgagg
     1201 ttcttatggg acattcctga gccgtcgcga agaactacag tatccgtatt gacgccgaat
     1261 tgcttcagct tctctttcat gacggcgagt cctgcactcc aactgccttg gccgcgctgc
     1321 actctcccca tttccttcac caaagtttca gcgtgcccgt tgttgctcaa cttcatgaat
     1381 ggaatgaaaa gctcattcag cggcatcgac tttttcgata caaggacagt tgcattgcct
     1441 ggtgcaatgc cagtctccat tccaccgttt ccgacaaatt cgatgccttg ttcggcgagc
     1501 gatttcttaa agacgtcgag cgttagccct gtcggctccc aaacagctga ccatgattgg
     1561 gatcttgtac cttctacagg aattttccct tcgattatga ttctgttcgt tccgtgctca
     1621 cgtgcgatgg aaatgctttt cgcttcgcct ttcgcgactg ttgttgcgcg gttgacgatt
     1681 tctacatagt cagtttccgg agtgagtgtc actttcggaa gatctccggc tttatccccg
     1741 gcatttactt cgacgatgac agttcccgca tcatagtctt cgttcggaga aagcgtcagt
     1801 gcagaaactt gtgctccgac gtaattatgc tcatccgacc agttcaaatc ctgtgaatag
     1861 cggtcatcgt cataccagct gtcgtctgca atcaggttgc cgttgatctt atcaatccct
     1921 ttagccttca aatcctttgc gaattggtcc aaatcctttt tcatcaacgt cgggtcgcct
     1981 ttacctttca gataaagatt cccatggatc attttcccct ttacttctcc gtccgtcagc
     2041 acttctgtgg aaaactgata atcagggccc aatatgtcca ttgccgtcga tcctgtcaac
     2101 aatttcatat tggaagcagg gcggagacgg atgtcaccat agtgggaata aatcttttcc
     2161 cccgtatcgg ctttccgaat actgactccg gttattcccc cttggagctt cgggtctttc
     2221 aagattgcct gtaatttttg atctaaagtt gtcgaatcaa tggatgctgc gggagttgta
     2281 tccccggatg cggcgacaga tgaaagagtt ccttcatgtg ctaccggaaa taaggcgatc
     2341 aaggcaatag ccattgccag gaaagccctt ttgagaagac attgcttttt cattaaaatg
     2401 ctcacatcct ttcatccttt gtatttgatt gtacttcaac ttcctgaagg tggacagatt
     2461 aaaatgcaga aaattagcaa ttgaatcatt ttacctaggt tggtaggtgt ttgctattaa
     2521 ttatatgtac agaggtgctt tgcccataca ttcaagaaaa gaaataacag gttgaagcga
     2581 acagtgattc aggtagaata atatatattc atacgataag gaagtgtcgg aaaaatgcag
     2641 tacactttgt ctcaattggg cgaccaagct gttgtcattg aagttggtga aaagattgac
     2701 gcatcatccg aacggcaagt gaggacgatt gtatcgctat tggaacagaa atcaccgcca
     2761 tggatgattg aatatatacc cgcctatacg acagtcaccc ttatttactc gatacgggaa
     2821 ttccttaaag aagatagtcc ttatgaagcg gtttgtgcgc gtatccgtga attgctcaaa
     2881 gatgtgaaag aaacagctgc tgttcaacaa aaggttgtgg aaatcccagt tttgtatggc
     2941 ggagaattcg gaccggatct ggattttgta gcggagcata acgggttaac accggacgaa
     3001 gtcattgcaa tccatacgtc gggtgagtat atggtacata tgattggctt tgcgccgggc
     3061 ttcccgttta tcaggggcat gtcagaacag attgccgctc cgagaaggaa gacgccacgg
     3121 ccgaagattc ctgcccgttc ggtaggaatt gcgggattgc aaacaggggt ttatccaatt
     3181 gaaacgccgg gaggatggca gttgatcggc aggacgccgc ttgacttatt ccttcctgaa
     3241 gaagatcctc caagcttgtt gtcagcgggg aataaaatta ggttttacaa gattacgagc
     3301 gaggagtata aagacatgag aggtggagcg ggtgattgag gttacgaagc cgggccttca
     3361 tgatacagtg caggatgtag ggagattcgg atatcagaag ttcggtgttg ttgcgggcgg
     3421 cgctatggat ccgttctctc accgggtcgc gaatatgctt gttggcaata acgagacgga
     3481 agcaacactt gagatgacac ttctcg
//
LOCUS       DASULI010000177         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1806078,
            whole genome shotgun sequence.
ACCESSION   DASULI010000177 DASULI010000000
VERSION     DASULI010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806078"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="VFX34_03865"
     CDS             complement(<1..1782)
                     /locus_tag="VFX34_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5564073.1"
                     /translation="MNRIWHSIVGKLWATILLLVSFVLFMVTVLLLEFLDNFHTNQAE
                     DSLRREATTIGKIVLDHENDSAMQLIIQDILDNETNAMIIDSEKKVYYSFHRGLNQEK
                     IERKIMSEPKFFSGIQINEKVVKEMILPSLAEENAMEQFLVLMYPYAEVDGTIHSIVM
                     YQSLDAVHRTTKRTTHIVFLSAFIAFVLTTIFAFFLSTKITSPLRGMKQAAFELSKGN
                     FETRLPVMQNDEIGQLATAFNQMGRQLKYQMELIKQEKEQLSSILTSMTDAVITFNRD
                     YSILLSNPQAERLLQKWYFANGADDKVLPKEIFHMLEHAITFAEEVEDELEIGGQFYG
                     ISISPLYSENSIRGAVAVLRDMTDQHKLDKLRSDFIANVSHELRTPISLLQGYSEAII
                     DDVMTSEEERNEMVQIIHDESKRMSRLVTDLLDLARMESGHMRLYKLNFPLVPFLDRV
                     RNKFMQIARDSNVSLTLNFDESDKEIILFADDDRLEQVFTNLIDNAIRHTPDGGKVTV
                     GVERKGDAVEITVADTGSGIPKEDLPFIFERFYKADKARTRGKGGTGLGLAIAKNIID
                     SHEGRITAELGETSGTVFKCILPIGPVA"
     gene            complement(1775..2494)
                     /locus_tag="VFX34_03870"
     CDS             complement(1775..2494)
                     /locus_tag="VFX34_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017553608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5564074.1"
                     /translation="MEETVRLLVVDDEDRIRRLLNMYLSREGFEIDEAVDGAEAIEKA
                     LANHYDCILLDLMMPEKDGLEVLQELRDEKKMTPVILLTAKGEESDRVAGFETGADDY
                     IVKPFSPREVVLRVKAILRRSVTFPDATAANSSKDLVVFPQLTIDHDAHRVTAEGKEV
                     NLTPKEYELLYFLAKSPDKVFDREQLLKEVWHYEFFGDLRTVDTHVKRLREKLSRVSE
                     SAAKMIVTVWGVGYKFEVPNE"
     gene            complement(2598..3782)
                     /gene="ccsB"
                     /locus_tag="VFX34_03875"
     CDS             complement(2598..3782)
                     /gene="ccsB"
                     /locus_tag="VFX34_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX5564075.1"
                     /translation="MGLASLSANLLLVSFIAYLVATLFFGGAVKGAKSEASYKNHRWG
                     NVGITITIIGFITHLGYFITRWMASGHAPVSNMFEFTTAFGMMLVGSFILLFFLYRTP
                     SLGLFALPIAIIIIGYASMFPRDITPLIPALQSYWLTIHVITAALGESILAISAVAGL
                     IYLVKNVDMTKPSRQRFWLEAVMFTLILVLGFVLSSTTFKLTGYEAHFTYIDKNQAPA
                     KIEYNYPPLFGMHEYEAQTPGAMTPWAEMPAVINAKTLTTFVWSVATGTVLYLLLRLI
                     FRRPIASLFQPFAKKANSQLMDEIGYRSVLIGFPVFTLGALIFAMIWAHEAWSRFWGW
                     DPKEVWALITWLFYAAFLHLRLSKGWEGKKSAWLAVIGFVIIMFNLIAVNLIIAGLHS
                     YA"
     gene            complement(3782..>4443)
                     /locus_tag="VFX34_03880"
     CDS             complement(3782..>4443)
                     /locus_tag="VFX34_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX5564076.1"
                     /translation="KFDSYNIFQMDFRQGELKSMTMELIEKKTEKSFGQFTIDLDNPE
                     GIYELNEGARVDLIGYYADYDGVENGEPVSKSPIPNNPAFIFNMITPDKPKGEKSFVA
                     IRQTLETQENAYKVKFVSAETRDISGLTIRKDKTLYVLLLGGLIFMIGVIQGSYWNHR
                     RIWIQKGEGDELLVAAHTNKNWFSLKKELNAVKDYSGLPKYEDRQDNESSTEEEEGEK
                     L"
BASE COUNT         1166 a         1069 c          943 g         1265 t
ORIGIN      
        1 agctacaggg ccgatcggca aaatgcattt gaagaccgtg cctgatgttt caccaagttc
       61 tgctgtaatc cgcccttcat gtgagtcgat gatattcttt gctattgcta gccccaaacc
      121 tgttccgcct ttccctcttg tcctcgcttt atccgcttta taaaaacgtt caaagatgaa
      181 aggcaggtcc tctttcggta tgccgctgcc tgtatcagcg acggtaattt caacggcatc
      241 cccttttctt tcgacaccaa cggtcacttt tcctccatca ggtgtatggc ggatcgcatt
      301 atcgatcaga ttagtaaaaa cttgctctaa tcggtcgtca tctgcgaaaa ggatgatttc
      361 tttatcgctt tcgtcaaagt tcaatgttag cgatacattt gaatctctgg cgatttgcat
      421 gaatttattg cggactctat ccagaaaagg aacaaggggg aaattcaatt tgtataagcg
      481 catatggccg gattccattc gggcaaggtc aagcagatcc gtaacgagcc ggctcatccg
      541 tttcgattca tcgtggatga tttgcaccat ttcattgcgt tcctcttcgc tcgtcatgac
      601 atcatctatg atcgcctcac tgtatccttg caataaggag atcggcgtcc tcagttcgtg
      661 tgatacgttg gctatgaaat cggatctgag cttatccagt ttatgctgat cggtcatgtc
      721 acgcaacacg gcaacagctc cgcgaatact attctcactg tatagcgggc taatggagat
      781 gccgtaaaac tggccgccga tctcgagttc atcctcaact tcctctgcga atgtgatcgc
      841 atgttcaagc atatgaaaaa tttccttcgg caacactttg tcatctgcgc cattggcaaa
      901 ataccatttc tgcaatagcc gttcagcttg ggggttgctt aatagaatcg agtagtcccg
      961 attgaatgtg atgacagcat ctgtcattga ggtgaggatg cttgataatt gttccttttc
     1021 ttgcttgatc aattccattt gatatttcaa ttgcctgccc atctgattga aagcggtcgc
     1081 aagttggccg atttcatcat tctgcatgac tggcaaacgc gtttcaaaat tccctttcga
     1141 caattcaaat gccgcttgct tcattccccg caaaggagaa gtgattttcg tggaaaggaa
     1201 gaaagcgaaa atggtcgtta aaacgaaggc gatgaatgcg gataaaaata cgatatgtgt
     1261 cgtccttttc gtcgtacgat ggacagcatc aagactttgg tacatgacga tagaatggat
     1321 ggttccatcg acctcggcat atggatacat tagaacgaga aactgttcca tggcattttc
     1381 ttccgcaaga gatgggagaa tcatttcttt tacgactttt tcattaatct gaataccaga
     1441 aaaaaacttc ggctccgaca tgattttccg ctcaattttc tcctggttca aacctctatg
     1501 aaacgagtaa tagacctttt tttcagagtc gatgatcatc gcattcgttt cgttatccaa
     1561 aatgtcctgg atgatcagtt gcattgcgga atcgttttca tggtccaaaa cgattttacc
     1621 gatcgttgtc gcttcccgac gcagtgaatc ctcagcttga ttcgtatgga aattatcaag
     1681 gaattcaagg agcaggaccg tgaccatgaa aaggacaaag gaaacgagaa gcaatatggt
     1741 tgcccatagc ttcccgacga ttgaatgcca tattctattc attcggaacc tcgaatttat
     1801 agccgacgcc ccaaactgtg acaatcattt tggcagcact ttccgaaaca cggctaagct
     1861 tttcccgcag ccttttcaca tgtgtatcga ctgtcctcag gtctccgaaa aattcgtaat
     1921 gccatacttc cttcaataat tgctcgcggt cgaatacctt gtcgggcgat tttgcaagga
     1981 aatagagcaa ttcgtattcc ttcggtgtca aattcacttc tttgccttca gccgtcaccc
     2041 ggtgtgcatc atgatcgatt gtcagctgag ggaaaacgac aagatccttt gaactgttcg
     2101 cagccgttgc atccgggaaa gtgaccgatc ttctcaatat cgctttcaca cggagcacaa
     2161 cctcccgcgg actgaaaggt ttgacgatat agtcgtccgc gcctgtttcg aagcccgcaa
     2221 cccggtccga ttcctcgcct ttcgcagtca gaagaatgac gggtgtcatt ttcttttcat
     2281 cacgaagctc ctgcaatact tcaaggccat ctttttccgg catcatcaaa tcaagtaaga
     2341 tgcagtcata atgattagcg agcgcctttt caatcgcttc ggctccatct accgcctcat
     2401 cgatttcgaa gccttcgcgg gataaataca tattgagaag acggcggatc cgatcctcat
     2461 cgtccaccac taataatctt acagtctctt ccataatcaa tttccccttt caactacact
     2521 tctgtcctta ttgtacaagt agtcaataaa aaaagaaact gcacagctcc tcttcggctc
     2581 tgtgcagtcc aatcggttta tgcgtatgaa tgcaaaccgg cgatgatcaa gttcacggca
     2641 atcagattga acatgatgat gacaaatccg ataaccgcaa gccatgcaga tttcttccct
     2701 tcccagcctt ttgaaagccg gagatgcagg aaagctgcgt aaaacagcca tgtaattaat
     2761 gcccatacct ctttcggatc ccagccccaa aatcttgacc aagcttcatg cgcccagatc
     2821 attgcaaaaa tcaaagctcc aagggtgaat actggaaaac cgatcagtac ggaacggtac
     2881 ccgatttcat ccataagctg cgagttcgct tttttggcga acggttggaa caaggatgca
     2941 atcggtctac ggaagataag ccgtaagagt agatatagaa ctgttcctgt agcaaccgac
     3001 caaacaaacg tcgtcaatgt tttcgcattg atgacggcag gcatttcagc ccacggcgtc
     3061 atcgctccgg gggtttgcgc ttcatattcg tgcattccga atagcggcgg gtagttatat
     3121 tcgattttcg caggtgcctg gtttttatcg atatacgtaa aatgggcttc atatcctgtt
     3181 agcttgaaag tcgttgatga tagtacgaat ccaaggacta agatgagtgt gaacatgact
     3241 gcttctagcc aaaatcgttg cctggaaggc ttggtcatat caacattctt cactaggtaa
     3301 ataaggccgg ccactgcact gattgcaagg atagattcac ctagcgctgc cgtgatgacg
     3361 tggattgtca gccagtagct ttgaagtgcc ggtataagcg gtgtgatatc ccgagggaac
     3421 atgctcgcgt atccaatgat gataattgcg ataggcaatg cgaacaatcc taaggatggc
     3481 gtccggtaca agaagaacag caggatgaaa gatcctacaa gcatcatgcc gaatgcagtc
     3541 gtaaattcaa acatattact gacgggggca tggcctgatg ccatccatct cgtaatgaaa
     3601 tatccaagat gggtgatgaa gccgatgatg gtgatcgtaa tcccaacatt tccccaccta
     3661 tgatttttat aggatgcttc cgatttagcg cctttcactg caccgccaaa aaacaaagtg
     3721 gcgaccaaat aggcaatgaa agagactaaa agcaaattcg cgcttaacga tgctaatccc
     3781 attataattt ttctccttcc tcttcctcag ttgacgactc attatcctgc ctgtcttcat
     3841 atttcggcaa gcctgagtaa tcttttacgg cattcaattc ctttttcaac gagaaccagt
     3901 ttttatttgt gtgggcggca accaacaatt catcgccttc ccctttctgg atccaaatcc
     3961 gtctatgatt ccaataagaa ccttggatga cgccgatcat gaaaataagt ccgccaagta
     4021 gaagaacata caatgtcttg tctttccgga tggtcaaccc ggaaatatcc ctggtctccg
     4081 cactgacgaa tttcacttta taagcattct cttgcgtttc cagcgtttgc ctgattgcga
     4141 caaagctctt ttcgcctttt ggtttatcgg gagtgatcat gttgaagatg aaagccgggt
     4201 tgtttggaat cggcgacttg gaaaccggtt cgccattttc aaccccatca taatccgcat
     4261 aatagccgat caaatcgaca cgggctccct cattcagttc ataaatccct tctggattat
     4321 caagatcgat ggtgaattgg ccgaaggatt tctctgtttt cttttcgatg agctccatcg
     4381 tcatcgattt cagttcacct tgtctgaaat ccatctggaa gatgttgtag ctatcgaatt
     4441 tca
//
LOCUS       DASULI010000178         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1208695,
            whole genome shotgun sequence.
ACCESSION   DASULI010000178 DASULI010000000
VERSION     DASULI010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208695"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..825)
                     /gene="recX"
                     /locus_tag="VFX34_03885"
     CDS             complement(13..825)
                     /gene="recX"
                     /locus_tag="VFX34_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEX5564077.1"
                     /translation="MPVITKITQQKRDSERYNIFLDETYAFSVHESVLVKFGLTKGMQ
                     LDEWSMDEITYEDQIEKAFNRALHYLSFRMRSEFEVKKKLLDLEYGEAVVLEAIVKLR
                     RLGFLDDEAFSEALLRTQKNASNKGPKAIQQELQKKGVGKELQQQVLENYSEEEQFAV
                     AKKLAEKAARSNSSIAPAQLKQKIQNALARKGFSFDLISSVMEEIDFGRDEDEWDSII
                     SNVGEKAWRRYQSKHSGYELRKRVKQAMYQKGIPFERIDSFIEKKENEEDGD"
     gene            902..1798
                     /locus_tag="VFX34_03890"
     CDS             902..1798
                     /locus_tag="VFX34_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX5564078.1"
                     /translation="MKVVIAGGSGFIGQHITDLLLKNGHEIIILSRSKKTDSENVKYV
                     LWLQEGAVPEKEIGGADVFINLAGVSINDGRWTETHQKRIYESRMEATDELIRIIGEL
                     DEKPTLFINASAIGIYPASETAIYTEDYSEVATDLLGRTVSDWEKKAASLNGFGIRTV
                     FTRFGVVLWKDEGALPLMVLPYKMFVGGKVGTGRQWVSWVHVKDVAKAVLHIIQNDQL
                     EGAVNVTAPFPQRMDDFGKMIGAALHRPHWFPTPSPLMKLALGKKSSLVLEGQYVTPE
                     KLLANGFEFDFPVLSSALDNLL"
     gene            1856..2659
                     /locus_tag="VFX34_03895"
     CDS             1856..2659
                     /locus_tag="VFX34_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5564079.1"
                     /translation="MVEEHGPGILMAAFVIVVGILFNPFAAKAEEFHWGFKKATNGVP
                     PNAGPALESMLDKHGAIYKGKPDEKVAYLTFDNGYENGYTEKILDALKKEEVPATFFL
                     TGHYLKSAAPLVQRMVKDGHIIGNHSYGHPNMANLSEARMEEEWKKFDELLYQTAGVK
                     RTVYARPPEGIFNDAVLSKGNELGYRHIFWSVAFIDWHANKPRGRDYAYNELMNQLHP
                     GALILMHTVSPDNAQALPDFIRDAKKAGYEFRSLDDLVLEYENVNSILR"
     gene            complement(2825..>3115)
                     /locus_tag="VFX34_03900"
     CDS             complement(2825..>3115)
                     /locus_tag="VFX34_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5564080.1"
                     /translation="ILASLLMLERKFQLRNIKRILVVTNSFHMKRLDLTLRTYMPSWI
                     NFTLCPTQDGVTHRNNWHLSEKGRIRVLTEAEKIINYVKQGSIIDEKIDFTL"
BASE COUNT          787 a          645 c          726 g          957 t
ORIGIN      
        1 tcactatatt ttttagtctc catcttcttc gttctccttt ttttcgatga aactgtcgat
       61 ccgttcgaat ggaatccctt tttggtacat cgcttgtttt acccgtttac ggagttcata
      121 tccggaatgc ttggactgat aacgacgcca cgctttctca ccgacgttgc tgatgattga
      181 atcccattcg tcttcatctc tgccgaagtc gatttcctcc atgacgctgc tgatcaggtc
      241 gaaggagaat ccttttcttg caagtgcatt ttggattttt tgctttagtt gtgcaggagc
      301 gattgaactg ttcgaccttg ccgctttttc ggctagtttt ttcgcgactg caaattgttc
      361 ttcctctgaa tagttttcaa gcacttgctg ttgcagctcc ttcccgaccc cttttttctg
      421 caattcttgt tggatcgctt ttggaccttt attcgatgca tttttctgtg ttcgcagtaa
      481 tgcttcggaa aatgcctcat catcgaggaa tccgagtctt cttaatttga cgatcgcttc
      541 aaggacgacg gcttctccgt attccaagtc gagcagtttc tttttcactt cgaattcgct
      601 tcgcattctg aaggataggt agtggagtgc acggttgaat gctttttcga tttggtcttc
      661 gtatgtgatt tcgtccatcg accattcgtc aagctgcatt ccttttgtca gtccgaattt
      721 gacgaggacg gattcgtgga cgctgaatgc ataggtttcg tccaggaata tattataacg
      781 ttcgctatca cgtttttgtt gtgttatttt cgttataaca ggcaaatcga ttcgacctcc
      841 ctcgctatct ataagtatac atgttctgtt cgaagaggtc gaaccttgga ggttgatcgg
      901 aatgaaagtc gttattgctg ggggttcagg tttcattgga caacacataa cagatttact
      961 attgaaaaac ggccatgaaa ttatcattct ttcgaggagt aagaagacgg acagcgaaaa
     1021 tgtgaagtat gtgctttggt tgcaggaagg tgctgttccg gagaaagaga ttggcggagc
     1081 ggatgtgttt attaatcttg caggagtttc cattaatgat gggcgttgga cggaaaccca
     1141 tcagaaacgg atttatgaaa gcaggatgga agcgacggat gaattgatcc ggatcattgg
     1201 cgagctggat gaaaaaccga ctctctttat taatgcgagt gcaatcggaa tttatcctgc
     1261 ttctgaaacg gccatttata cagaggatta ctctgaagtt gctacggatc tgcttggcag
     1321 gacggtcagt gattgggaga agaaagctgc ttcgctgaac ggcttcggga ttcgtactgt
     1381 tttcacgcgg ttcggagttg ttctttggaa ggatgaaggg gctttgccgc ttatggtctt
     1441 gccgtataaa atgtttgtcg gcggaaaagt cggcaccgga cggcagtggg tttcatgggt
     1501 gcatgtgaag gacgttgcca aggctgttct gcatatcatt caaaatgacc aattggaagg
     1561 tgcagtgaat gttaccgctc cttttccgca gcggatggac gatttcggca agatgatcgg
     1621 tgctgctttg catcgcccgc attggtttcc tacaccttct ccacttatga agctggcgct
     1681 cgggaaaaaa agttccctcg ttctggaagg acaatatgtg acacccgaaa agctgttggc
     1741 gaatggcttt gaattcgatt ttcctgttct atcctcagct ttggataatc ttctgtaata
     1801 ggagtatatt ccatttcaat ccgcacatcc tactcaaaaa ggagatgtgc aatctatggt
     1861 tgaagaacat ggaccaggaa tactgatggc tgccttcgtg attgttgtcg gtattttatt
     1921 taatccgttc gcagcaaaag ctgaagaatt ccattggggc ttcaaaaaag cgacgaatgg
     1981 cgttcctccg aatgctggac ccgctctgga atcaatgctt gataaacatg gcgccattta
     2041 taaaggtaaa ccggacgaaa aagtcgctta tttaacattt gataatggct atgaaaatgg
     2101 ctatacggaa aagattttgg atgcgctgaa aaaagaggaa gtcccagcca cgttcttcct
     2161 gacgggacac tatttgaaaa gtgccgcccc tcttgtccaa cggatggtga aggacgggca
     2221 tatcatcggc aatcattctt atggacatcc gaatatggcg aatctatccg aagcaagaat
     2281 ggaagaggaa tggaaaaagt ttgatgagtt gctttatcaa acggcaggcg tcaaacggac
     2341 ggtgtatgca agaccgcctg aggggatttt caatgatgcc gtattaagca aaggcaatga
     2401 gttgggctac cgccatattt tctggtccgt cgctttcatt gattggcatg ccaataaacc
     2461 gcgcggccgt gactatgctt ataatgagct tatgaatcag cttcatccgg gggcactcat
     2521 actcatgcac actgtttcgc ctgataatgc gcaggcgctg ccggatttca tccgcgacgc
     2581 caagaaagcg ggatatgaat tccggtcatt ggacgatctt gtcctcgaat atgaaaacgt
     2641 caattcgatt ttacgatgaa tccgaaagca tcccctacga taacagatcg taagtagata
     2701 tcacactaca actcaaagac cgccaattcg aattagaatc gcggtctttt ttaacgcctt
     2761 tagttaggca gtgaaacgtg caataatggc ttactttata gccttaactc aattaatgcg
     2821 aaatttacaa tgtaaaatcg attttttcat ctattataga accttgtttg acatagttaa
     2881 tgattttttc cgcctcagtt agaacgcgga ttctcccctt ttcacttaaa tgccagttat
     2941 ttcgatgggt aacgccatcc tgagttggac acaaggtgaa gttgatccaa cttggcatat
     3001 aagttcttaa tgtcagatct aaccttttca tatgaaagga atttgtcact actaaaattc
     3061 tttttatgtt gcgaagttga aacttccttt ctaacatcaa taaagatgct agtat
//
LOCUS       DASULI010000179         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1356871,
            whole genome shotgun sequence.
ACCESSION   DASULI010000179 DASULI010000000
VERSION     DASULI010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356871"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFX34_03905"
     CDS             <1..492
                     /locus_tag="VFX34_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HEX5564081.1"
                     /translation="ALDYIDVIQIGARNMQNFELLKEAGMVNKPVLLKRGLAATIDEF
                     INAAEYIISKGNSQIMLCERGIRTYERATRNTLDISAVPILKQETHLPVFVDVTHSTG
                     RKDLLLPTAKAAIAVGADGVMAEVHPDPAVALSDAAQQMDIKQFDEFYAEIERFMNTH
                     QTV"
     gene            727..1725
                     /gene="ccpA"
                     /locus_tag="VFX34_03910"
     CDS             727..1725
                     /gene="ccpA"
                     /locus_tag="VFX34_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEX5564082.1"
                     /translation="MAVTIYDVAREANVSMATVSRVVNGNQNVKPATRKKVLECIERL
                     GYRPNAVARGLASKKTTTVGVIVPDISKSYYAELSRGIADIATMYEYNIILSNSDKRA
                     TREVELLEDHLGKQVDGLIFMSDSLSDEIRAEMATANVPIVLAGTLGDNTDLPTVNID
                     HEEAAYEAVKKLIDNGHERIVHVSGPMSRDINRLCKKVGYERALKEAGLPLIEEYFIE
                     TDNTYDDAYEKWAVIRELEEKPTAIFAGNDEIAVGVLNGIRDEGYSVPDDYEVICFQH
                     TLLSRAVRPQLTSIIVPLYDLGAVSMRLLTKLMNGEEVDDINVILPYHKEVRQSTK"
     gene            complement(1776..>2423)
                     /locus_tag="VFX34_03915"
     CDS             complement(1776..>2423)
                     /locus_tag="VFX34_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX5564083.1"
                     /translation="AHHGDGVQWTFYDDPNVCTLSIHETGRYLFPGTGAVTERGNGEG
                     YGTSFNFPIDAFTEDESFLEIYKTAIEEVAAFFKPDVILTQNGADAHYFDPLTHLYGT
                     MKIYEQIPRIAREIAERYCGGRWIAVGGGGYDIWRVVPRAWSHIWLAMKQLPAPTGRL
                     PEQWLNAWQAESPVPFIETWKDPDPLYEPIPRKAEITEKNAQMLERALHIVRSQQ"
BASE COUNT          661 a          544 c          618 g          600 t
ORIGIN      
        1 gcgctcgatt atatcgatgt catccaaatc ggtgcgcgca atatgcagaa tttcgaattg
       61 ctgaaggaag cgggcatggt gaataaacca gtccttctga aacgcggatt ggctgcgacg
      121 attgatgaat tcatcaatgc ggctgaatat atcatttcca aagggaatag ccagatcatg
      181 ctatgtgagc gtggaatccg cacatacgag cgggcaacac gaaacacttt ggacatttcc
      241 gcggttccga ttttaaagca ggaaacgcat ttaccggttt tcgtagacgt cactcactcc
      301 acgggcagaa aagacttgtt gcttccgact gccaaagcgg caattgctgt aggtgcggac
      361 ggcgtcatgg cagaagtgca tcctgatccg gctgtcgctc tatccgatgc ggcacagcaa
      421 atggacatca aacagttcga cgagttctac gctgaaattg aaagattcat gaacacccat
      481 caaacagtct aacttaatcc aacccggcca ggctggattg agttagacgc ctccggcgga
      541 tgtcacggat tttaggggag tttatcgaac ttctcgttaa aaatccggac gcaattacgc
      601 caaggcgtaa ttgattggct gctacatttg aattattcta taaacagcgc cggacctttc
      661 aaggatccgg tgttttgttt ataatgaaag taagcgaata catactgatg ggaaggagat
      721 tcgtcaatgg cagtaactat atatgatgta gcaagggaag cgaatgtttc catggctacg
      781 gtttcccgtg ttgtcaatgg caatcagaac gtaaaacctg caacacgtaa aaaggtgctc
      841 gagtgcattg aacgtctcgg ctatcgtccg aatgctgttg caagaggatt ggcaagcaag
      901 aagacgacga cggttggcgt catcgttccg gatatttcga aaagttatta tgcggaacta
      961 tcacgcggaa tcgcagatat tgcgacgatg tacgaataca atatcattct gtccaactcg
     1021 gacaagcggg cgacgaggga agttgaacta ttggaagacc atctcggcaa acaagttgat
     1081 ggtctcattt tcatgagtga ctcactctca gatgaaatcc gtgcggaaat ggcgacagcg
     1141 aacgtgccga ttgttttggc cggcacattg ggggacaata cggatttgcc gaccgtgaac
     1201 atcgatcatg aagaagcagc ttatgaagcc gtgaagaaat tgattgataa tggccatgag
     1261 cgaatcgttc atgtaagcgg tccgatgtcg agggatatca atcggttgtg caagaaagtt
     1321 ggatacgaga gggcattgaa ggaagcgggc ctcccgctca tagaagaata tttcattgaa
     1381 acggataata cgtatgatga cgcttatgaa aagtgggcag tcattcgcga gcttgaagaa
     1441 aagccgaccg ccatttttgc cggaaatgat gaaattgcag taggcgtttt aaatggaatc
     1501 agggatgagg gctattctgt gccggatgac tatgaagtga tctgtttcca acatactttg
     1561 ctgtcgcgtg cagtgagacc gcaattgacc tcgatcattg tcccgttata tgacttggga
     1621 gctgtttcga tgcgattgtt gacaaagctg atgaacggcg aggaagtcga cgatataaat
     1681 gtcatcctgc cgtaccataa ggaagtacgt caatcgacga aatgaaaaag ctgttccgga
     1741 aaaactcccg gaacagcttt ttctcggata aaggctcatt gctgacttcg cacgatatgt
     1801 aatgcgcgtt cgagcatttg ggcatttttc tccgttattt ccgctttgcg tggaattggt
     1861 tcgtacaatg gatcaggatc cttccatgtt tcgatgaatg ggaccgggga ctcggcctgc
     1921 caagcattga gccactgttc cggcaatctt ccagtcggag caggcagttg cttcatggca
     1981 agccaaatat gcgaccacgc cctcgggacg acacgccata tatcatagcc tccgccgccg
     2041 acagcaatcc atcgcccccc gcaatatcgt tcggcaattt cacgcgctat ccgtggtatt
     2101 tgttcataaa tcttcatcgt accgtacaaa tgggtgaggg ggtcaaaata atgtgcatcc
     2161 gccccgtttt gagtcaatat gacatcaggc ttgaaaaaag cggcgacttc ttcaatggcc
     2221 gttttataaa tctcaagaaa ggactcgtcc tccgtaaaag catcaatcgg gaaattgaag
     2281 gaagtcccat aaccttcgcc atttccacgt tccgtgacag cgcccgtccc cggaaaaagg
     2341 tatcttccag tttcgtggat ggacagtgtg cagacattcg gatcatcgta aaatgtccat
     2401 tgcaccccgt cgccatgatg cgc
//
LOCUS       DASULI010000180         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_914662, whole
            genome shotgun sequence.
ACCESSION   DASULI010000180 DASULI010000000
VERSION     DASULI010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914662"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFX34_03920"
     CDS             <1..682
                     /locus_tag="VFX34_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="X-prolyl-dipeptidyl aminopeptidase"
                     /protein_id="HEX5564084.1"
                     /translation="EDIVPFKNEYVFEADVLPAFQAIDLKWIAEPKVASNGSGQLIFS
                     LKELGFNLTGEELADVVVSDTNLPKELAGKTFVGIGSSVLRAVHQAGLLEGFEYKTTR
                     SGHEGLIKATVDANHPLMAGYRQDELFYTTRGSWITAVPEGAEVLATVKATDDFYVAG
                     WWPGHEGAKGQTLAFTQQLDDSTITLFANDLAFRAHTKHYYRMLANSIYAAAEEAPAP
                     KPGAGKQH"
     gene            complement(1103..>2182)
                     /locus_tag="VFX34_03925"
     CDS             complement(1103..>2182)
                     /locus_tag="VFX34_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5564085.1"
                     /translation="LSHNGGSMVSPKSIEADYAAMKAGDAAGTVIATNPVGTGFFKFE
                     SWEPGSQIKLVKNEDYWGDKAHVDSVTFKVVPESAVRNADLEAGYVHIADPVQPNEVA
                     DINSKDFAKINQKASSSLAYLGFNTEKEPFNDPKVRRAISMLVNKDDISEGVYDGFGV
                     AAKGPLAPGIFGYNEDAKPIEYNVEEAKKLLKEAGYEKGFKSSIWTNDNPQRQQIAVL
                     LQNELKKVNVEVEIEVMEFGAYLDKTANGEHDMFILGWSNPTGDADYGMYALFHSSQK
                     GDPGNRSFYENPEVDKLLEEGRRESNPDERIKIYNKVQDILIEDAPMAYLIHTEYLTG
                     VSNKIRGFSIGTDGIYKLKDVQFVE"
BASE COUNT          537 a          529 c          526 g          590 t
ORIGIN      
        1 cgaagacatc gttccgttca agaacgagta tgtattcgaa gcagacgtac tgcctgcctt
       61 ccaagcaatc gatttgaagt ggatcgcaga gccgaaagtg gctagcaacg gatccggcca
      121 attgattttt tcattgaagg aattgggatt caatttgacg ggtgaggaac tggccgacgt
      181 cgtcgtgtcc gacacgaatc tgcctaagga gcttgcgggc aaaacgttcg tcggtatcgg
      241 cagcagcgtt ttgagggcgg ttcatcaagc aggattgctc gaagggttcg aatacaagac
      301 gacaagaagc ggacatgaag ggctgatcaa ggcgacggtc gatgcgaacc acccgttgat
      361 ggccggttat cgtcaagatg agctgttcta tacgacaaga ggttcatgga tcaccgctgt
      421 tcctgaagga gcggaagtgt tggcaacagt taaagcgacc gatgatttct acgtcgccgg
      481 ctggtggccg ggccacgaag gcgcgaaagg acaaaccctt gccttcaccc agcaattgga
      541 cgattcgacc atcacgcttt tcgcaaacga ccttgcattc cgcgcacata cgaaacacta
      601 ctaccgcatg ctggcaaaca gtatttacgc tgcggcggaa gaagccccgg cacctaagcc
      661 cggagctggt aaacaacact gaaatacgaa cagccctgta gactattcta cagggctatt
      721 ttgttttgaa gcgtctctaa atttcggtgc ctgtgcagca aactattcag attatgcact
      781 acaattgcat gaagaattcc aggaatccaa ttataccaat caaagaagag tctaaatatg
      841 tatgaacatc aatctggttg aatattcata aatgcatatt gcacaggcac cgacacaatt
      901 ctgattatgc actacaattg cataatgaaa aaaaggaagc cgaccgtttc aatcgttggt
      961 tggcttcctg ttgcattaat atacttgcgc ttgaattgtc ataaatgcgt cctgcacagg
     1021 caccaacaca atggcaccaa cacaattcac ccgcacgcaa aaaaagaatg ccgccctcaa
     1081 ggcggcatcc ttcccgtcat tattactcca cgaactgaac gtctttcaac ttatagatac
     1141 catccgtgcc gatggagaag cctctgattt tattgctgac gcctgtgagg tattccgtat
     1201 ggatcaaata tgccatcggt gcgtcttcaa tcagaatgtc ttggactttg ttgtagattt
     1261 tgatgcgctc atccggattt gattcacggc ggccttcttc gagaagtttg tcgacttccg
     1321 gattttcata gaatgaacgg tttcctgggt cacccttttg ggatgagtgg aagagtgcgt
     1381 acattccgta gtcggcgtcc cctgtagggt tggaccagcc gaggatgaac atgtcatgct
     1441 cgccgttcgc cgttttatcg agataggcgc cgaattccat tacttcaatt tcgacttcca
     1501 cgttgacctt cttcaattca ttctgcaaaa ggacggcgat ttgttgacgc tgcgggttgt
     1561 cgttcgtcca gatggaagat ttgaagcctt tttcgtagcc tgcttccttc aaaagttttt
     1621 tcgcttcttc cacattgtat tcgatcggtt ttgcatcttc gttatagccg aagataccag
     1681 gggcaagagg tccttttgca gccactccga aaccgtcgta tactccttcg ctgatgtcat
     1741 ctttattcac gagcatggag attgctcggc gcacttttgg atcattgaac ggttcttttt
     1801 ccgtattgaa gcctaaatac gcaaggctgg acgatgcttt ttggttgatt ttcgcaaaat
     1861 ctttactatt gatatcggca acttcgttcg gttggaccgg atctgcgata tggacgtagc
     1921 cagcttcaag gtctgcattc cggaccgcgc tttcaggaac gactttgaat gtcaccgaat
     1981 ctacatgggc tttgtcgccc cagtagtctt cgtttttgac aagcttgatc tgagagccgg
     2041 gctcccagct ttcgaatttg aaaaagcctg ttccgacagg gtttgtggcg atgacagtac
     2101 ctgctgcatc tccagctttc atcgctgcat aatccgcttc gatcgatttc gggctcacca
     2161 tgctgccgcc gttatgcgaa ag
//
LOCUS       DASULI010000181         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1429236,
            whole genome shotgun sequence.
ACCESSION   DASULI010000181 DASULI010000000
VERSION     DASULI010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429236"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFX34_03930"
     CDS             <1..585
                     /locus_tag="VFX34_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HEX5564086.1"
                     /translation="GVGSAPNAAAAASVSHPAKQGLVQMLSVFIDTLLICSATAFMLL
                     SSGVVPTEGMAGVPYAQAALSSTFGEFGIWFITFALFCFAFTSLIGNFFYAESNLKYL
                     NKKEVGKTGLTLFRLAAVVIVFFGAQLDFSVAWNTADVLMGIMALINIPIILILGGTY
                     FKCIDDYTRQKKEGKNPIFKAKSIGLKEKTDFWN"
     gene            607..1671
                     /locus_tag="VFX34_03935"
     CDS             607..1671
                     /locus_tag="VFX34_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HEX5564087.1"
                     /translation="MFPLVRVNKKHLLANVRAVTELCAAKNIAVTGVTKVFGGDPEIA
                     AVYVEGGITRLGDSRVENLKLLKGLDAEKWLIRPPMISEVDDLVAYADASLNSELATI
                     QAINDAAIAKGKVHKVILMVDLGDIREGFIDHDELIDVAVETENMEGVHLYGIGVNLT
                     CFSFIQSDSEKMAALTELSKTVGRRVGRELEVVSGGNSATLDLMMKDGITEGVNNLRL
                     GESLLFGKERANYTFLPGTHKDVFILECEIVELKEKPSMPWGTVGVDSYGNSPTFTDQ
                     GMRMKAICALGKQDFDVETSVPVDQDIIILGASSDHLMLDVTDSKRTYKVGDTIELQL
                     GYFATMRAFTSRYVHKIYVG"
     gene            complement(2001..>2820)
                     /locus_tag="VFX34_03940"
     CDS             complement(2001..>2820)
                     /locus_tag="VFX34_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564088.1"
                     /translation="TMPTEEHTGELTAFNDKDGLFYSVYNSGFTEDGGYASNIRFGNG
                     DGFETIRIPHYLQMLGKEDGDLLMVIGNDTHITLQKMPLQKDVQLEEIVSLGPNGERV
                     GLASILKEGDYYYLLMADTEKLTSDVYRIHEKTKEIETFPFFRYKDLEDYIARIPYNL
                     RNAATIHDGILYYVDGMGDVHAVDSTTGEVDLAFTLEGASQGQRKMSEQTYFSDGKLH
                     FFRYHESSGTHAIDTYDLRTGNLLSELPIEGLDAMFNYARQKKKRIAPYDFIVR"
BASE COUNT          701 a          663 c          718 g          738 t
ORIGIN      
        1 ggtgtcggtt ccgcaccgaa cgcagctgcg gcggcaagcg tatctcatcc ggcgaagcaa
       61 ggtcttgtcc aaatgctgtc ggtgttcatc gatacgctat tgatctgttc ggctacagct
      121 ttcatgctat tgagctcggg tgtcgtgcct accgaaggca tggcgggcgt tccttacgcg
      181 caagcggccc tttcatcgac attcggtgaa ttcggtattt ggttcatcac gtttgcattg
      241 ttctgctttg cgttcacatc gctaatcgga aacttcttct atgcagaatc gaatcttaag
      301 tatttgaata aaaaagaagt aggcaagaca ggtcttacgc tcttcagact ggctgcggtc
      361 gtcatcgtct tcttcggcgc acaacttgat ttctcagttg cttggaacac tgccgacgtc
      421 ctgatgggca tcatggcatt gatcaacatt ccgatcatct tgatcctcgg cggcacttac
      481 ttcaaatgta ttgacgatta tacgagacag aaaaaggaag ggaaaaaccc tatcttcaag
      541 gcgaagtcga tcggtcttaa agagaaaacg gatttctgga attaattatt gattagaggg
      601 attgatatgt ttccgttagt tcgtgtgaat aaaaagcatt tgcttgcaaa tgtccgtgcc
      661 gtcacggagc tgtgcgcagc gaagaatatt gccgtcacag gcgtgacgaa agtgttcgga
      721 ggggaccctg aaattgcggc tgtgtatgtc gagggcggca ttacgcggct cggagattca
      781 cgggtcgaaa atttgaagct actgaaaggg ctggatgcgg agaaatggct catccgcccg
      841 ccgatgataa gcgaagtgga cgacctcgtc gcgtacgcgg acgcttcgct caattcggag
      901 cttgcgacaa ttcaggcgat caatgacgcc gcgattgcaa aagggaaggt ccataaagtg
      961 attctgatgg tggatcttgg cgatatccgt gaagggttca tcgatcacga cgagcttatt
     1021 gatgttgcgg tggaaaccga gaacatggaa ggcgtccatt tgtatggcat cggggtcaac
     1081 ttgacgtgct tttcgttcat ccagtccgac tcggagaaga tggccgcact tacggaactg
     1141 tcgaaaacgg tcggcaggag ggttggcagg gagttggaag tcgtcagcgg cgggaattcc
     1201 gcgaccctcg acttgatgat gaaagatggc atcactgagg gagtcaacaa tttgcgcctc
     1261 ggcgaatccc tgttgttcgg caaggagcgc gccaactaca cgtttctgcc cggaacgcat
     1321 aaagacgtgt tcatattgga atgcgaaatc gtcgaattga aggagaagcc atccatgcca
     1381 tgggggacag tcggtgtcga ttcctacggc aacagcccga cattcaccga ccagggcatg
     1441 cggatgaaag cgatctgcgc ccttggaaaa caggatttcg acgtcgaaac atctgtgccg
     1501 gtcgaccagg acatcatcat tcttggtgcc agctccgacc acctaatgct cgacgtcaca
     1561 gacagcaaga ggacatacaa agtcggcgac accatcgaac tccaactcgg ctacttcgcc
     1621 accatgcgcg ccttcacctc ccgctacgtc cacaaaatct acgtgggttg actagggttg
     1681 agtagaaatt aagtagaaat tgagtaggtg cctgtgcacg gcacatttat gacaattcaa
     1741 ctttcattat aattatccag agagaggcca tccgaagatt gaatcggata gcctctctca
     1801 ttattttata caattgtagt gcatagtcag aattgttccg gtgcctgtgc aaggtgcaat
     1861 tatgataatt cgatatctag aatataaata cataaaaaag ccaatcagta cttgatatga
     1921 ttggctttcg aaatttactt atgcaattgt agtgtattgt ctgaatagtg tgctgtgcag
     1981 gcaccgtatt cacccaatat tcatcttaca ataaaatcat acggggcaat gcgttttttc
     2041 ttttgccggg catagttgaa catggcgtcc agtccttcga tcggcaattc gctgagcaag
     2101 ttgcctgtcc ttaagtcata tgtgtcgatt gcgtgcgttc cggacgattc atggtagcgg
     2161 aagaagtgga gcttaccatc actgaaatac gtttgctcgg acattttcct ttgtccttgg
     2221 ctcgcccctt caagcgtgaa tgcgagatcg acctctccag tagttgaatc gactgcatgc
     2281 acatctccca tcccgtccac ataatacagg atgccgtcat ggattgtggc ggcgttccgg
     2341 aggttatacg gaatgcgggc aatataatct tcgagatcct tgtacctgaa gaaaggaaac
     2401 gtctcgattt ccttcgtctt ttcgtggatg cggtacacat cgcttgtcag cttttccgtg
     2461 tcagccatca atagataata atagtcgcct tcttttagaa tcgatgccaa tcccacccgt
     2521 tcaccattcg ggccgagcga gacgatttct tctagctgga catctttttg aaggggcatc
     2581 ttttgcaatg taatgtgggt atcattaccg atcaccatca gcagatcccc gtcttccttg
     2641 cccaacattt gcaaataatg ggggatgcgg attgtttcga agccatcccc atttccgaac
     2701 cgaatgttgg aagcatagcc gccatcttcg gtgaagccag agttatagac gctgtaaaag
     2761 agaccgtcct tatcattgaa agcagtcaac tcaccagtat gttcctccgt cggcatggtg
//
LOCUS       DASULI010000182         3236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_428276, whole
            genome shotgun sequence.
ACCESSION   DASULI010000182 DASULI010000000
VERSION     DASULI010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428276"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFX34_03945"
     CDS             complement(<1..653)
                     /locus_tag="VFX34_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5564089.1"
                     /translation="MLYDCVIIGGGPAGLNAALVLGRARRNVLLFDDNHARNRVTREA
                     HGFITRDGILPDEFRRLAHRDIGNYPSVEIKSEKVMSIKRLGSIHFELETAEGNSFQT
                     IKIILATGLKDEQPNIPNIEKFYGTSMFSCPYCDGWELRDKALAVIADKNVYKLAKEV
                     FVWSKDLAVFTNGEGRLEADECDKLLSKGIHVYEDSIARLEGENGQLKGICLEDGTL"
     gene            772..921
                     /locus_tag="VFX34_03950"
     CDS             772..921
                     /locus_tag="VFX34_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564090.1"
                     /translation="MEKKMLERGIGLGTCLAVVISWSVHKSILWAIFHGILSWLYVIY
                     YALTR"
     gene            987..1142
                     /locus_tag="VFX34_03955"
     CDS             987..1142
                     /locus_tag="VFX34_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564091.1"
                     /translation="MKSYAGDKDEKPNGLPKDSAGKANDAARKAEEQLESIKQEARNE
                     MKRQQKK"
     gene            1460..2356
                     /locus_tag="VFX34_03960"
     CDS             1460..2356
                     /locus_tag="VFX34_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5564092.1"
                     /translation="MKLRVSAVQYHLHAIRSFDEFAAQCEHYIRNAQEYGAEFVLFPE
                     FFTTQLLSIGDENGKALSIEALPGFTNQYIELFTSLAKETNMHIIGGTHVIQREGRLY
                     NVAHLFFPDGTVEEQAKLHITPFEIEGWNMAAGDELKVFDTEKGRIAILTCYDIEFPE
                     IVRMVKAAGADVIFCPSCTDDRHGFHRVRYTSHARAIENQVYVVLTGTVGSLQTVDFM
                     RANFGQAAIITPNDIPFPPKGLAAEGELNHDMLVTADLDLNLLYEVREKGSVTTWRDR
                     RTDLYTDWGAKPIGSESNGISQ"
     gene            2340..3014
                     /locus_tag="VFX34_03965"
     CDS             2340..3014
                     /locus_tag="VFX34_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564093.1"
                     /translation="MAYRSEVMVSHNGGFIPVVVRNYTRADFNAMIALQADCFPPPFP
                     EELWWNREQLENHVSLFSEGAVCVEYDGRIIGSLTGLLVRFDSEEPDHTWEDMTDGGF
                     IRNHDPDGDTLYIVDICISPEFRKAGLGKWMMQAIYQLAVKLDLERVLGGGRMPGYGK
                     VAGELTAQQYVEKIVQGSLKDPVITFLLRCGRMPVKLIEDYLEDEESHNNALLMEWKN
                     PFRRGK"
     gene            3017..>3236
                     /locus_tag="VFX34_03970"
     CDS             3017..>3236
                     /locus_tag="VFX34_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564094.1"
                     /translation="MEFIRVNNIEDPLFAKMHTLLGKIFPPEEVLEFDLWKEPLNDPS
                     IRVFVAVHEGEVVGATEYRYYPDWNIAMT"
BASE COUNT          797 a          743 c          858 g          838 t
ORIGIN      
        1 atgagcgttc cgtcctccaa gcagatccct ttcagctggc cattttcgcc ctccagtcgg
       61 gcaatactgt cttcataaac atgaatgcct ttcgaaagca gcttatcaca ttcatccgcc
      121 tcgagccgtc cttcgccatt cgtgaacacg gccaaatctt tgctccacac aaaaacttcc
      181 ttcgcaagct tgtatacgtt cttatccgca atgacggcaa gcgccttgtc ccggagctcc
      241 catccgtcgc agtacggaca gctgaacatg ctcgtcccgt agaacttttc gatgttcggg
      301 atgttcggct gctcgtcctt caagcctgtc gccaaaatga tttttatcgt ttggaagctg
      361 ttgccttctg ccgtttccag ttcgaaatgt atactgccga gccgtttgat ggacatgacc
      421 ttttcgcttt tgatttccac agatggatag ttgccgatat cccgatgagc aagtcgccgg
      481 aactcgtcgg gcaggatgcc gtcgcgggta ataaatccat gggcctcgcg cgtcacccgg
      541 ttccgggcat gattatcatc gaatagcagc acgtttcttc tcgctcttcc aagcaccagg
      601 gcggcattca aaccagccgg cccaccgccg ataatgacac agtcgtatag catgaatcca
      661 ttcatccttt ttagtgcaag tttacccttc gtgaatgaaa ataagcgtca tgcaaagtta
      721 actttcatga aagaaggcgg ccgtacttat actggactag ggagggatga gttggagaag
      781 aagatgttgg agcgcggcat cggtcttggc acttgtttgg cagtcgtcat ttcctggagc
      841 gtgcacaaat cgattctttg ggcaatcttc catggaatcc ttagctggct gtatgttatt
      901 tattacgcgt tgacgagatg atctgcaggt ttatgatgga cccttttttg ggaatactat
      961 aggtatcaac aaaaggaggc gcatctatga aaagctacgc tggcgataaa gacgaaaagc
     1021 caaatggact tccgaaagac tctgcgggaa aggcgaacga tgcagcccgg aaagcggagg
     1081 agcagttaga gtcgatcaaa caagaagcgc ggaatgaaat gaaaagacag caaaagaaat
     1141 agagtttacc acggatgcct gccaatttcg cgggcatcct tttcattttt gcgtaatctt
     1201 taccggttat gctcgagttt gaggttgttt tgcgcgaatt cggacaggtt atgcgcaagt
     1261 ttgccgttct tttgcgcgat ttcacctcgg ttatgaccca attacaccga gttatgcgca
     1321 acttttacat acttatgatc atttcgggac ctttgacccg aaagactacc agcccccgat
     1381 caatttccaa aaaaatgaat gactttacca cttccatata ttatgatgtt gctaatcaca
     1441 tataataggg ggtttgtcaa tgaaacttcg tgtttccgct gtgcaatatc atctgcatgc
     1501 catccgttcc tttgacgagt tcgcggcgca atgtgagcac tatatccgaa atgcgcagga
     1561 gtacggcgca gagtttgttt tattcccgga attcttcacg acgcagctgc tttccatcgg
     1621 ggatgaaaac ggaaaagcgc tttcgattga ggcgctgccg ggctttacga atcaatacat
     1681 cgaactgttc acatcgctcg cgaaagaaac gaacatgcat attatcggcg gcactcatgt
     1741 catccagcgt gaggggcgcc tttataatgt cgcccatctt ttcttcccgg atggcacagt
     1801 cgaggagcaa gcgaaattgc acatcacccc attcgaaata gaaggctgga atatggctgc
     1861 cggcgatgaa ttgaaagtgt tcgacacgga aaagggcaga atcgccattt tgacgtgcta
     1921 tgatatcgaa tttcctgaaa tcgtccggat ggtgaaggcg gcgggggcgg atgtcatttt
     1981 ctgtccttcc tgcaccgacg accgtcatgg atttcatcgc gtcagataca cgagccacgc
     2041 gcgggcgatt gaaaaccaag tatatgtcgt gttgacgggg actgtcggtt cgttgcaaac
     2101 ggtcgatttt atgcgtgcga acttcgggca agctgcaatc atcacaccga atgacattcc
     2161 gtttccgccg aaagggttgg ctgcggaagg cgagctgaat cacgatatgc ttgtcacggc
     2221 tgatctcgac ttgaacctgt tgtacgaagt gcgtgagaaa gggtctgtga cgacttggcg
     2281 cgatcgcaga accgatttat atacagattg gggagcaaaa ccgatcggga gcgagagcaa
     2341 tggcatatcg cagtgaagtg atggtctccc ataatggggg cttcattcct gttgtcgtcc
     2401 gcaattatac acgagcggat ttcaatgcca tgattgcttt gcaggcggat tgctttccgc
     2461 cgcctttccc ggaggagctt tggtggaacc gggaacagtt ggaaaatcac gtaagcttgt
     2521 tctcggaggg tgccgtctgt gtcgagtacg atggaagaat aataggttcg cttacgggct
     2581 tgctcgtcag gtttgattcc gaagagcctg accatacgtg ggaagacatg acggatggcg
     2641 ggtttattcg aaaccatgat ccggacgggg acacgctcta tattgtcgac atttgcatta
     2701 gcccggagtt ccggaaggcg gggctcggca aatggatgat gcaggcaatc taccagcttg
     2761 cggtcaagct tgatcttgag agggtcctgg gcggcggccg gatgcctggc tacggaaagg
     2821 tcgccgggga attgactgct cagcaatatg tagagaagat agttcaaggc agtttgaagg
     2881 acccggtcat tacgtttctg ctccgctgcg ggcggatgcc cgttaaattg attgaagatt
     2941 atttagagga tgaagagtcg cataacaatg cattattaat ggaatggaag aatccgtttc
     3001 gtagggggaa ataaagatgg aatttattcg agtgaacaat attgaagatc cgctatttgc
     3061 gaagatgcac acattattgg ggaagatttt tccgccggaa gaggttttgg aatttgactt
     3121 atggaaagag ccgctcaatg acccaagcat ccgtgtcttt gtcgcggtgc atgaagggga
     3181 agtggtcggc gcgaccgaat atcgttatta tccggattgg aatattgcga tgaccg
//
LOCUS       DASULI010000183         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_759904, whole
            genome shotgun sequence.
ACCESSION   DASULI010000183 DASULI010000000
VERSION     DASULI010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759904"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..620
                     /locus_tag="VFX34_03975"
     CDS             180..620
                     /locus_tag="VFX34_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEX5564095.1"
                     /translation="MTMDFNFYMEDITKQARSEMEASGYEQLTTPEEVEAAFKRPGTT
                     LVMVNSVCGCAGGIARPAAAHAIHYDKRPDHLVTVFAGQDKEATAYVRMIFGEDHLPS
                     SPSFALLKDGKLVAEVGRYEIEGHDPMSVVTNLQSQFEEHCEEI"
     gene            complement(650..745)
                     /gene="prli42"
                     /locus_tag="VFX34_03980"
     CDS             complement(650..745)
                     /gene="prli42"
                     /locus_tag="VFX34_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HEX5564096.1"
                     /translation="MSNKKVQKVIVYIMIAAMVASSVLIGLSVFL"
     gene            860..1279
                     /gene="mce"
                     /locus_tag="VFX34_03985"
     CDS             860..1279
                     /gene="mce"
                     /locus_tag="VFX34_03985"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX5564097.1"
                     /translation="MKKVDHIGIAIKDLNNALPYYIETLGLKLLGIEEVKSQKVKVAF
                     IDAGNCKIELLEPIGEDGAIAKFLEKRGEGIHHIAFGVSDIRTRMQELKEKGVQMLQD
                     EPKPGAGGAQVAFMHPKSSFGVLYELCDKGDKPNGHL"
     gene            1266..2813
                     /locus_tag="VFX34_03990"
     CDS             1266..2813
                     /locus_tag="VFX34_03990"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX5564098.1"
                     /translation="MDIYDKINELYDRKREIELGGGDERIARQHEKGKLTARERINLL
                     LDEGTFVELNPFVIHRTRDFGMDKQMGPGDGVVTGYGKIDGRPVYLFSQDFTVFGGAL
                     GEMHAMKIANVMDLAAKNGAPFIGLNDSGGARIQEGVVSLDGYGEIFYRNAIYSGVIP
                     QISVILGPCAGGAVYSPAITDFVFMTDETSQMFITGPKVIETVTGEKISSEDLGGSKV
                     HNSISGNAHFRGKDEKTVLEQVRNLLSYLPQNNEEKAPILEGRFEEDYRNDLADVVPF
                     ETIRPYDVRKVIEQVVDENSFLEVQAEFAKNAVVGFARMKGESVGLICNQPRVMAGGL
                     DINSSDKIARFIRTCDSFNIPLITFEDVTGFFPGVKQEHGGIIRHGAKILYAYSEATV
                     PKITVILRKAFGGAYVALNSKAIGADVVYAWPNAEIAVMGAEGAANIIFAREIANSDD
                     PDSMRAEKIEEYRAKFANPYVAASHGMVDDVIDPRETRIKLLQALEMMRNKQETRPKK
                     KHGNIPL"
     gene            2839..>3123
                     /locus_tag="VFX34_03995"
     CDS             2839..>3123
                     /locus_tag="VFX34_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564099.1"
                     /translation="MVNAQRLLDEFFELVQINSETKHEEGIVTVLKRRMEDLGFTVVE
                     DDSKKVTGHGAGNLIATLKGTVDGADPIYFTCHMDTVVPGKGIKPELREDG"
BASE COUNT          933 a          622 c          800 g          768 t
ORIGIN      
        1 aaaaaagagc tcgtagagga acttgaaaac cggttgctta ccggtgaaat gacggttgcg
       61 ggcgctgtgg attccattct tccgatggaa taacaaaacc aatctttgca atccgctttt
      121 ttgcaagtgt aacaaccaac tgctatgatg aaaatatatt ttgaaagggg ccgatccgaa
      181 tgacaatgga ttttaatttc tatatggaag atattacgaa gcaagcgcgg tcagaaatgg
      241 aagcaagcgg ctacgaacag ctgacaacac cggaggaagt ggaagcggca ttcaaacgcc
      301 cgggcacaac gctcgtcatg gtgaactccg tttgtggatg tgcaggaggc attgcacgtc
      361 ccgctgcagc acacgctatc cattacgata aacggcctga tcatcttgtc actgtgttcg
      421 ctggacaaga caaggaagcg actgcatacg tacgcatgat ttttggtgaa gaccatctgc
      481 cttcgtcccc atcatttgct ttattgaaag atggaaaact tgttgctgaa gtgggccgct
      541 atgagattga aggtcatgat ccaatgtccg tcgtaaccaa cttgcaaagc caatttgaag
      601 agcattgtga ggaaatataa aaaacagtcc accaaggact gtttttttct tataaaaata
      661 cgcttaaacc aataaggaca cttgaagcga ccatagccgc aatcattata tagacgatta
      721 ctttctgcac ttttttattg ctcatcgggc taatctcctc accgttatag atacttccat
      781 tttaacagat tccttaaaat tctcaagatg tagcggattt aattagaaaa cgttacaatg
      841 gaatgaagga ggcgttcaaa tgaaaaaagt ggatcatatc ggaattgcca ttaaagattt
      901 gaataatgct ctgccctatt acatagaaac gttaggattg aaattgcttg gaattgaaga
      961 ggttaaaagc caaaaagtga aagtggcttt catcgatgca ggcaattgta agatcgagct
     1021 acttgagccg atcggagagg atggggcgat tgctaaattc ctcgaaaaac ggggggaagg
     1081 catccatcat atcgcattcg gtgtttccga cattcggaca cgtatgcaag agttgaagga
     1141 gaagggcgtc caaatgctgc aggatgaacc aaaaccagga gctggcggcg ctcaagtggc
     1201 cttcatgcat ccgaaatcat ctttcggggt gctgtatgaa ctatgtgaca aaggggataa
     1261 gccaaatgga catttatgat aaaatcaatg aactgtacga taggaaacgg gaaattgaac
     1321 tgggcggcgg tgatgagcgg attgccagac agcatgaaaa agggaaactg acagcccggg
     1381 aacggatcaa tcttttgctc gacgaaggaa cttttgtcga attgaatcct ttcgtcatac
     1441 ataggacacg cgattttggc atggacaaac agatgggacc tggcgatggc gtcgtcacag
     1501 gatatgggaa gatcgatggg cgtccggtct atttgttctc acaggatttc accgttttcg
     1561 gcggtgcgct tggtgaaatg cacgctatga aaattgcgaa cgtcatggat ttggccgcga
     1621 aaaatggtgc gcctttcatt ggcttgaatg attctggcgg cgctcgtatt caagaagggg
     1681 tcgtttcact ggatggatac ggcgaaatct tctaccgcaa tgcaatttat tcaggcgtca
     1741 ttccacaaat ttccgtcatt ttaggtccat gtgcaggtgg agcggtctat tctccggcta
     1801 tcactgattt cgtgttcatg acagatgaaa cgagccagat gttcatcaca ggacctaaag
     1861 tcattgagac agtgacgggt gaaaaaattt catccgaaga tttgggtgga tcgaaagtac
     1921 ataattccat tagcgggaat gcacacttcc gcggaaaaga cgagaagact gtattggagc
     1981 aagtgcgaaa tcttctttcc tatcttccgc aaaataatga agaaaaggcc cctatccttg
     2041 aagggagatt tgaagaggac tatcgaaacg atttggcgga tgtcgtgcct tttgagacga
     2101 tacgccctta cgatgtccgc aaagtcattg aacaagttgt cgatgaaaat tcatttctgg
     2161 aagtacaggc ggaattcgcc aagaatgcgg ttgtcggctt cgccagaatg aaaggcgaat
     2221 ctgtcgggtt aatatgtaac caaccaagag taatggcagg cggattggat attaattcat
     2281 cagacaagat tgcccggttc atccgaacgt gtgattcatt caatatcccg ctcatcacgt
     2341 ttgaagatgt cactggattc ttcccgggcg tgaagcagga gcacggcggc attatccgtc
     2401 atggtgcgaa aatcctgtat gcttattcgg aagcgacagt ccctaaaatt acagtcattt
     2461 tacgaaaagc attcggcgga gcttatgtcg cattgaactc caaagcgatc ggcgcggacg
     2521 ttgtgtatgc atggccgaat gctgaaatag ccgtcatggg ggctgaaggg gcggcaaaca
     2581 tcatatttgc ccgtgaaatc gccaatagtg atgatccgga ttcgatgagg gcggaaaaaa
     2641 tcgaagaata tcgtgcgaag tttgcaaacc cgtatgtcgc agcttcccac ggaatggtcg
     2701 acgatgtcat cgatccgaga gagacgcgca tcaaattgtt gcaagcgctt gaaatgatga
     2761 ggaataagca agagacaaga ccgaagaaaa aacatggcaa catcccattg taagggaact
     2821 cgaacgaagg agcgatgcat agtgaatgca caacggctat tggatgagtt ttttgagctt
     2881 gtacaaataa attccgaaac gaaacatgaa gaaggaattg tcactgtatt gaagagaaga
     2941 atggaagacc ttgggttcac agttgttgaa gatgactcca aaaaggtgac tgggcatggc
     3001 gccggaaacc ttattgcaac attgaaaggc acagtcgatg gcgcggatcc gatttacttt
     3061 acatgccata tggatactgt cgttccagga aaagggatca aacccgaatt gcgtgaagat
     3121 gga
//
LOCUS       DASULI010000184         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1426693,
            whole genome shotgun sequence.
ACCESSION   DASULI010000184 DASULI010000000
VERSION     DASULI010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426693"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1532)
                     /locus_tag="VFX34_04000"
     CDS             complement(189..1532)
                     /locus_tag="VFX34_04000"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016203805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HEX5564100.1"
                     /translation="MTTYDLIIKNGKIVTTESVIQGDIAIKDGKIQEVSQSLEASAER
                     VIDAEGLHVLPGLIDTHVHFNEPGRTEWEGIATGSRSLAAGGATSFFDMPLNSTPPTI
                     NKENLDLKKACADEKSVVNPYFWGGLVPENIADLKELHENGVIGFKAFMSPSGIADFN
                     HVDDETIFKGMKEIASFGSLLAVHAESTVICDQLAEEKQKQGKTSARDFVESRPIISE
                     IEAVRRIISYAEATGCKLHVVHASSRKVVDIITEAKQRGVDITVETCPHYLSMTVKDL
                     EEKGGLAKCCPPLRDEDEVEDLWAAIANGEINVIASDHSPAPASMKTITDNYFEGWGG
                     ISGAQSTLNIMLTEGHFKRNLPLEKIVELTATNPAKLFGLANKGAIETGFDADIAIVD
                     LNESFELKNEDLFYRHQHSPYVGMTFKGKVKTTIVNGEVVFENDEIVAEKTTVAQ"
     gene            complement(1604..2962)
                     /gene="allB"
                     /locus_tag="VFX34_04005"
     CDS             complement(1604..2962)
                     /gene="allB"
                     /locus_tag="VFX34_04005"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX5564101.1"
                     /translation="MAFDVVIKGGNVVLRDGVYKVDIGIKDEKISCIAERITDDAKEV
                     IDATGQYVMPGMIDTHVHISEPGRSEWEGFETGSKSLAAGGTTSYVEMPLNALPATTN
                     KAALDLKLEAAVDQNYVDYAFYGGLVPGNVDKLKEMSDAGVLAFKCFLSDITSDIPDD
                     FVNVDDFTLYKGMQKLAELDQILCIHAENAAVPSGLAKEFAAEGKNSGVEYAESRPIF
                     TEVEAVQRAILFAKETGCKLHLVHISTSEAIQTILKAREEGVDVTVESCPHYFAFTAD
                     QVDEVGPKAKCQPPIRKAEDQARLWDELLAGNIDWLTSDHSPCTEDLKQGTIWEAWGG
                     ISGAQNNVDLMFDLAVKQRGLPVHEFVKLISTNPSERFNITHKGEIAVSKDADIILVD
                     PNQSYTVKREDLYYKNKHSAYEGRKIDCRVTKTIVRGNVVFDLEKGIVGEPVGKLISA
                     SK"
BASE COUNT          715 a          749 c          655 g          913 t
ORIGIN      
        1 tggactgagc gcagctaggg aagcggctga agccgtgccc gcggaaagtg tccgaccgta
       61 gcggaaatca acctttctag tcttaacgca atgttaatag aaaatgactg taggcaaaca
      121 ccaaaatccc tggtgtttgt ctacagtcta aaaaaaccaa agcccggttg ggctttgatt
      181 tttcattttt attgagcaac ggttgttttc tcagcgacga tttcatcgtt ctcaaagaca
      241 acttccccgt tgacgattgt tgttttcact tttcctttga acgtcatgcc gacatatggt
      301 gaatgctggt gacggtagaa aaggtcttca tttttcagtt cgaagctttc gttcaagtca
      361 acgattgcga tatcagcgtc aaagccggtt tcgattgcac ctttattcgc aaggccgaat
      421 agcttcgcag ggttcgtcgc tgtcaattca acgatttttt ccaatggaag gttacgtttg
      481 aaatgccctt ccgtcaacat gatgttcaat gtggactgtg caccggaaat tccgccccaa
      541 ccttcaaaat agttgtctgt tattgttttc atagatgctg gagcaggaga atggtcggat
      601 gcaatgacat tgatttcccc attggcgatt gcagcccata gatcttcgac ttcgtcttcg
      661 tcgcgcagtg gaggacagca tttcgccaat ccgccttttt cctctaaatc ttttactgtc
      721 atagagaggt agtgagggca agtctccact gtaatatcga cgccgcgttg tttagcttcc
      781 gtgatgatgt ccactacttt tctgctgctg gcatggacaa cgtgcaattt acatccagtc
      841 gcttctgcat acgagatgat tcttctgacg gcttcaattt cagaaatgat cggtcttgat
      901 tcaacaaagt cccttgcgga cgtcttgcct tgcttttgct tttcttcagc aagctgatca
      961 cagatgactg tgctttctgc gtgaactgca agaagcgaac cgaaagaagc gatttctttc
     1021 atccctttga aaatcgtttc atcgtcaaca tggttgaaat cagcgattcc gcttggtgac
     1081 atgaatgctt taaatccgat aaccccattt tcatgaagtt cttttagatc tgcaatgttt
     1141 tccggaacaa gtcctcccca gaaataagga ttaacgacgg atttctcatc ggcacatgcc
     1201 tttttcaagt caagattttc tttattgatc gtcgggggcg tactgttcaa tggcatatcg
     1261 aagaaagaag ttgctccgcc ggcagccagg ctgcggcttc ccgttgcaat cccttcccat
     1321 tctgttcttc ccggctcatt gaagtggaca tgtgtgtcaa tcaaaccagg caacacatgc
     1381 agtccttctg catcgatcac gcgttcagct gacgcttcga gtgattggga aacttcttgg
     1441 attttgccgt ccttaatcgc gatatcccct tggataacgg attctgtagt gacaatctta
     1501 ccgtttttaa taatcaaatc atatgtagtc atctttcatt cctccatgta gatattgccc
     1561 ttcgccattg ccgattctga caatggcgag gggattgaat gaatcattta gaagcagaga
     1621 tcaacttgcc gaccggttcc ccgacgattc ctttttcgag atcgaagacg acgtttccgc
     1681 ggacgattgt ttttgtcacg cggcagtcga tttttctgcc ttcgtatgca ctatgtttgt
     1741 ttttgtagta aaggtcttcc cttttcaccg tatacgattg gttagggtcg actaaaatga
     1801 tgtccgcgtc ttttgaaacg gcaatttcac ctttatgggt aatattgaag cgttcggacg
     1861 ggtttgttga aatcaattta acgaattcat gaaccggcag tccgcgctgc ttcacagcca
     1921 agtcaaacat caagtcgaca ttgttttgcg caccgctgat tccgccccat gcttcccaaa
     1981 tcgttccttg cttcaagtcc tccgtacaag gcgagtggtc ggacgtcagc cagtcgatat
     2041 tcccggctag aagctcgtcc catagtcttg cttggtcttc cgcttttctg attggcggct
     2101 gacattttgc tttcggaccg acttcatcga cttgatcggc agtaaaggca aagtagtgag
     2161 ggcaagattc aaccgttaca tccacgcctt cttcacgcgc tttcaagatt gtctggatcg
     2221 cctctgacgt gctgatgtga acgagatgca atttacagcc tgtttcttta gcgaatagga
     2281 ttgcccgttg aacagcttcc acttccgtga aaatcgggcg ggattccgca tattcaactc
     2341 cggagttttt gccttctgcc gcaaattcct tcgcgaggcc ggatggaaca gctgcgtttt
     2401 ccgcatggat gcagaggatc tgatccaatt cagccaattt ctgcatgcct ttgtacaagg
     2461 tgaaatcatc cacattgacg aaatcatccg ggatatcact tgtaatatcg gataggaagc
     2521 atttgaaagc gaggacgcca gcgtctgaca tctccttcag cttatcgaca ttcccaggga
     2581 ctagtccgcc ataaaatgcg tagtcgacat agttttggtc aacagccgct tccaatttca
     2641 gatccaacgc cgctttgttc gttgtagccg ggagtgcatt cagcggcatt tctacgtagc
     2701 tcgtcgtacc gcctgcagcc aatgatttag aacctgtttc gaagccttcc cattcagaac
     2761 gtcctggttc actgatgtgt acatgcgtgt caatcatgcc aggcatgaca tattggccgg
     2821 tcgcatcaat gacttccttc gcatcatctg taattctttc agcgatgcaa gagatctttt
     2881 catctttaat cccgatatca accttgtaca caccgtcacg caatacaaca tttccgcctt
     2941 taataactac atcaaatgcc atttgaaatt cccctttcaa ctaccaatga ttttatacct
     3001 atcaacttca caccaagcgt cagttttgcc at
//
LOCUS       DASULI010000185         5147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_280724, whole
            genome shotgun sequence.
ACCESSION   DASULI010000185 DASULI010000000
VERSION     DASULI010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280724"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFX34_04010"
     CDS             <1..897
                     /locus_tag="VFX34_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5564102.1"
                     /translation="SDFIAAYDLDGIRLTKLDGFDDAFLNEMIDAIHDAKQGAYVLTN
                     EESGAAFDLKPSDAKMQALRESFVRFDPDSSPMDMFKGNDEHDLLQFDDLTGSRFTYD
                     IVEARMFPPTRWKVAATALFTLPGVPLMTYGSEIAVNGKEAPESHPLFNFKTDMEFKD
                     RIGNLNLLRNESETLRSGDFEMLHNEDGFMVYTRTSEDETWLVAINNTSKTASFAVPA
                     EKIGQNKKLRGLLDSDLIREADDGVFRVVLERELAEVYIADEDKGFNTPYLVASILVY
                     VIFLGFLFLVIKKGRERRRTEK"
     gene            complement(966..1820)
                     /locus_tag="VFX34_04015"
     CDS             complement(966..1820)
                     /locus_tag="VFX34_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5564103.1"
                     /translation="MRIFADSGSDLPKSFFDENDVTLIPLRVLIDDKEGDDVLEIDSK
                     EVYDAIRNGKHPKTSQASPELFHSTWKELAASGEDGLYIAFSSELSGTYNTAVMMKDQ
                     VKEVNPNMNLVIIDSRCASLGHGLLVQEAVRLRDAGDDVRTIERKIRDMANHMEHLFT
                     VEDLDYLAKGGRVSKASAFIGGLLNIKPLLHVEGGKLVPIEKHRGRKKVLRRMLELIE
                     ERGVELSKQTIAISHGDDESTALEMKALIEEKFQPKNVEVQMIGSAIGSHSGPGTIAI
                     FFLNKFNQ"
     gene            complement(1844..2665)
                     /locus_tag="VFX34_04020"
     CDS             complement(1844..2665)
                     /locus_tag="VFX34_04020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX5564104.1"
                     /translation="MKPHLIVLDLDGTLLTDEKVISEETAHTLKRAEEQGHQVMIATG
                     RPYRASAMYYRELGLKTPIVNFNGAFVHNPTDRSWKTVHETISLPVVNDVVEAMQDFR
                     LHNIIAEVMDDVYLQYHDEKLLDIVGFGNPVIKQGDIRESLQVEPTSLLIQADPQNVE
                     PIRRHLAEVHAEAIDHRRWGAPWDVIEIVRHGLNKAVGISHVAKWLGIPRERIIAFGD
                     EDNDLEMIDYAGVGVAMANGIDRLKSIADEVTTLTNNEDGIAEVLKERLLLTKKI"
     gene            2868..3629
                     /locus_tag="VFX34_04025"
     CDS             2868..3629
                     /locus_tag="VFX34_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5564105.1"
                     /translation="MIVNEENWSNIPLLHIVDETIENSKLPTVIFLHGFTSAKEHNLH
                     YAYNIAKKGFRVLLPDAHLHGVRDEGLDEVQLSLRFWEIVLTSIEEMAVLHKELQDRG
                     VPKIGMGGTSMGGITTLGCLTAYPWIEAAAVMMGAPGYVELAKAQMAQFERRGFKLPI
                     TDEERKSLLDTLAIFDLTKQRKKLNNRPVYFWHGENDTTVPFEPTFNFYNAAKKDYAD
                     APERFEFMTDPTAGHAVSRTGMLRTAEWFASYLNE"
     gene            3680..3988
                     /locus_tag="VFX34_04030"
     CDS             3680..3988
                     /locus_tag="VFX34_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX5564106.1"
                     /translation="MSDDMKGSLMGALENVIDPELGIDIVNLGLVYDAELDEEGTAKV
                     TMTLTSMGCPMGPQIVANIKQELMELPEVKDADVNIIWNPPWSRDMMSRYAKMALGVR
                     "
     gene            4155..>5147
                     /locus_tag="VFX34_04035"
     CDS             4155..>5147
                     /locus_tag="VFX34_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5564107.1"
                     /translation="MQMRMDGVQEDQRTPLEQFGRNLVEDVKNGKMDPVIGRDEEIRN
                     VIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVKGDVPEGLKDKQIFELDMSSLIA
                     GAKYRGEFEERLKGVLKQVKDSDGEIILFIDEIHTIVGAGKTEGAMDAGNMLKPMLAR
                     GELYCIGATTLDEYRMYIEKDPALERRFQQVMVREPSVEDTISILRGLKERFELHHGV
                     RIHDRALVAAATLSDRYLTERFMPDKAIDLMDEASAMIRTEIDSMPQELDSVTRRIMQ
                     LEIEEQALRKEKDAISQTRLEALRTELQELKDSSAGMREQWNKEKEALRDIQMKR"
BASE COUNT         1326 a         1186 c         1347 g         1288 t
ORIGIN      
        1 tccgatttca tcgccgctta tgatttggac ggcatccgtc tgacgaagct cgatggtttt
       61 gacgatgcgt tcttgaatga aatgatcgac gcaatccatg acgcaaagca aggcgcttat
      121 gtattgacga atgaagaaag cggagctgcg tttgacttga agccgagcga tgcgaaaatg
      181 caagcgctcc gtgagtcatt cgtgagattc gatccggatt catctccgat ggacatgttc
      241 aaaggaaacg atgagcatga cttgctccaa tttgacgact tgacagggtc gcgcttcacg
      301 tatgatatcg tggaagcacg gatgttcccg ccgacccgtt ggaaggtggc ggcaacagca
      361 ctatttacac ttccgggcgt gccgctcatg acatacggct cggaaattgc ggtcaacgga
      421 aaagaagcgc ctgaaagcca tccgcttttc aattttaaga cggacatgga attcaaggac
      481 cggatcggca acttgaattt gctccgcaat gaatcggaga cactccgcag cggcgatttt
      541 gaaatgctgc acaacgaaga tggtttcatg gtctacacgc gaacttccga agatgaaaca
      601 tggcttgttg ctatcaacaa tacatcgaag acagcaagct ttgcagtgcc agccgagaag
      661 atcggccaga acaaaaagct gcgcggcctg ttggacagcg acctgatccg ggaagcggac
      721 gatggtgttt tccgagtcgt cttggagagg gagcttgcgg aagtgtatat cgcagatgaa
      781 gataaaggct tcaacacgcc ttatctggtt gcatcaatcc tcgtctatgt catcttcctc
      841 ggcttcctgt tcctcgtcat taaaaaagga cgggaaagaa gacggacgga aaaataaaag
      901 catacatgga aaatgccttt tcgggcttgg ttgcgaccat gtccgtaaag gcattttttg
      961 tttatttatt gattgaattt attcaagaag aaaattgcga ttgtgccggg tcctgaatgg
     1021 gatccgatgg cggagccgat catttggact tccacatttt tcggctggaa cttctcttcg
     1081 atgagcgctt tcatttcaag tgctgtgctt tcgtcatcgc cgtgactgat ggcgatcgtc
     1141 tgcttcgata attcaacccc gcgttcctct atcagttcga gcatgcgccg aagcactttt
     1201 ttgcggccgc ggtgtttttc gatcggcacg agctttccgc cttcgacatg aaggaggggt
     1261 ttaatgttca aaagtccgcc gatgaatgcg ctcgccttcg atacgcgtcc gcctttcgcc
     1321 aagtaatcaa ggtcttcgac agtgaatagg tgctccatat gattggccat atcacggatt
     1381 ttgcgttcaa tcgtacgcac atcatcgccc gcatccctga ggcggacagc ttcctggaca
     1441 agaaggccgt gtccaagcga agcgcagcgt gaatcgatga tgacgaggtt catgttcgga
     1501 ttgacttcct tcacttggtc cttcatcatg accgccgtgt tatacgtccc tgaaagctcg
     1561 gatgaaaaag cgatatacag gccgtcttct cctgaagcgg cgagttcttt ccatgttgaa
     1621 tggaaaagtt ccggcgaagc ttgcgatgtt tttggatgtt tgccattgcg gatggcgtca
     1681 tagacttcct tcgaatcgat ctcaaggaca tcatcgccct ccttatcgtc gatcaaaacg
     1741 cgcaatggaa taagtgtcac atcgttttca tcgaaaaatg atttcggtaa atctgaacca
     1801 ctgtcggcaa aaattctcat tgggagtccc ccttattttc tttttatatt ttttttgtta
     1861 ataataatcg ttctttcagc acttctgcaa tgccatcttc attattggtc aacgtcgtca
     1921 cttcgtccgc aatggatttc agcctgtcga tcccgttggc catagcgacg cctacgcccg
     1981 cgtaatcgat catttcaagg tcgttgtctt cgtccccgaa agcgatgatg cgctcccgcg
     2041 ggattccgag ccatttggcg acatgtgaaa tgccgaccgc cttgttcaaa ccgtggcgca
     2101 cgatttcaat gacatcccat ggcgcgcccc aacgtctatg gtcgatcgcc tcagcatgga
     2161 cttccgccag atgtcttcgg atcggttcga cgttttgcgg gtctgcctgt atgagcaaac
     2221 ttgtaggctc gacctgcaag gactcccgaa tgtctccttg cttgatgacg gggtttccga
     2281 agccgacgat atccaatagc ttttcatcgt gatactgcaa atacacatcg tccatcactt
     2341 cggcaatgat attatggagg cggaaatcct gcatcgcttc aacaacatca ttgacgacag
     2401 ggagggaaat cgtttcatgg accgtcttcc aggagcggtc cgtcggattg tggacgaatg
     2461 caccattgaa gtttacaatc ggcgtcttca ggccaagctc gcgatagtac atcgcacttg
     2521 cccgatacgg ccggccggtt gcaatcatca cctggtggcc ttgttcttca gcccgtttca
     2581 atgtatgtgc ggtttcctct gagatcactt tttcatccgt cagcaatgtc ccgtccaagt
     2641 cgaggacaat caaatgcggt ttcatagttc tgcccccttt tggatagttc tttgttatat
     2701 acccattttc ccgggcgctt catctcgttt ttgaagctta ccatgatatg atatccagtt
     2761 tatcgttatc tatcgaggca agtcaaaaca ttccggaaga aaaccgtcct ttttgttttt
     2821 ctactaaaac tttggtacga tttagaaaaa gtacagaagg tgtttgaatg attgtcaacg
     2881 aggaaaattg gagcaatatc ccattattgc atattgtgga tgaaacaatc gaaaatagca
     2941 aactaccaac cgtcatcttc ctgcacggct tcacgagtgc caaagagcat aacttgcatt
     3001 acgcatataa tatcgcaaag aaaggattcc gcgtcctgct gccggatgcc catttgcacg
     3061 gcgtccggga tgaaggcttg gatgaagtcc agctaagcct gcgtttctgg gagatcgtcc
     3121 tgacgtcgat cgaggaaatg gcagtcctgc ataaggaact gcaggataga ggcgtcccaa
     3181 aaatcggtat gggtggaact tcaatgggcg gcattacgac gctcggctgc ctgactgcct
     3241 atccgtggat tgaggcagcc gctgtcatga tgggtgcacc gggatatgta gagcttgcaa
     3301 aagcgcaaat ggctcaattc gaacggcgcg gcttcaaatt gccgatcacc gatgaagaac
     3361 ggaaatcatt gctcgataca cttgccatct ttgatttgac gaaacaaagg aaaaagctca
     3421 acaatcgccc cgtctacttc tggcatggcg aaaatgatac gaccgtccca tttgaaccga
     3481 cattcaattt ctataatgcg gcgaagaagg attatgcaga tgcacctgaa cgattcgaat
     3541 tcatgacgga tccgacagcg ggacacgccg tttcaagaac aggcatgctt cgtaccgccg
     3601 agtggttcgc atcttatttg aatgaatagg gcatccttgc tatgatgaag gaagaacaga
     3661 ttcggaaagg ggaatttcaa tgagtgatga tatgaagggc agcttgatgg gtgctctgga
     3721 aaacgtgatc gatcccgagc tcggaattga catcgtcaac ttggggcttg tttatgatgc
     3781 agagctggat gaagaaggca ctgccaaagt gacaatgaca ttgacatcaa tgggttgtcc
     3841 gatgggaccg caaatcgtcg ccaacatcaa gcaggaactg atggaattgc ctgaggtgaa
     3901 agacgcggac gtcaatatta tctggaaccc gccatggtcg agggatatga tgtcccgcta
     3961 tgcgaagatg gcgcttggtg tacgttaata gaacattaaa aggttgtctg actgtattgt
     4021 caggcagcct ttttgttttg tgtttattcc tatccggaca gggaaaaata agcatgcgga
     4081 atgctttact tattggaatg aaaagcatat aatttaatta atcaaagata gtcaaagtca
     4141 aactgttgag gtgaatgcaa atgagaatgg atggcgtaca ggaagatcaa cgtacaccac
     4201 ttgaacaatt tggacggaat ctcgttgaag atgtgaagaa cggaaagatg gacccggtga
     4261 tcgggcgcga tgaagaaatc cggaatgtca tcaggatttt atcaagaaaa acaaaaaata
     4321 atcccgtgtt gatcggtgaa cccggcgttg gtaagacggc gatcgttgaa gggcttgccc
     4381 aacggatcgt caaaggcgac gtgccggaag ggttgaagga taagcagatt ttcgaactcg
     4441 acatgagctc gcttatcgcc ggtgcgaaat accgagggga attcgaggaa cggttgaaag
     4501 gcgttttgaa gcaagtgaag gacagcgacg gggaaatcat ccttttcatc gatgaaatcc
     4561 acacaatcgt cggcgccgga aagacggaag gcgcgatgga tgctggaaat atgttgaaac
     4621 cgatgcttgc ccgcggtgaa ttgtattgca tcggggcgac gacgctcgac gaataccgga
     4681 tgtatatcga gaaggatccg gcattggagc gccgcttcca gcaggtgatg gtgcgagaac
     4741 cttccgtcga agacacgatt tctatcctgc gcggcttgaa ggaacgtttt gagctgcatc
     4801 acggagtccg catccatgac cgtgcactcg ttgcagcggc aacgttgtcg gatcgctatt
     4861 tgacggaacg gttcatgccg gacaaggcaa ttgacttgat ggatgaagcg agtgcaatga
     4921 tccggacgga aatcgactcg atgccgcagg aactcgattc ggtcacacgt cggatcatgc
     4981 agctggaaat cgaggagcaa gcattgcgga aagagaagga tgcgataagc cagacgcggc
     5041 tcgaagcgct tcgcactgaa ttgcaggagc tgaaggattc ttccgccggc atgcgggagc
     5101 agtggaacaa ggagaaagag gcgctgcgcg atatccagat gaaacgg
//
LOCUS       DASULI010000186         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1471030,
            whole genome shotgun sequence.
ACCESSION   DASULI010000186 DASULI010000000
VERSION     DASULI010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471030"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1338
                     /locus_tag="VFX34_04040"
     CDS             154..1338
                     /locus_tag="VFX34_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5564108.1"
                     /translation="MREKLMQLLESREDEMIQIRRYLHEHPELSFKEENTAQYIADFY
                     KGKDVDVQRNVGNGYGIIVTIKGGKPGKTIALRADFDALPILEETDVPFKSKNEGVMH
                     ACGHDAHTAYLLILADCLIQLKDSLAGTIKIIHQHAEEAPPGGAKSIVESGVLDDVEN
                     IFGIHVFPTHPAGFVGYHSGYSLAGRSFFKLVVQGVGGHGSSPHMANDAIVAAAHFVT
                     AVQTVISRRLNPFDHGVITIGSFDGKGTFNVIKDSVELEGDVRYMTVENQKLIANEVQ
                     RIVKGIEVEFDVQCELTYTADYPPLYNDPEVTEKVVASLKSLDDPDIKEVGEYPMLSG
                     SEDFAYYLEKIPGCFFYIGCKPKGVETAYFNHHPKFDIDEDCLLVAAKSVGQVVCSFY
                     EQ"
     gene            1366..>2527
                     /locus_tag="VFX34_04045"
     CDS             1366..>2527
                     /locus_tag="VFX34_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5564109.1"
                     /translation="MKSQLMELLESRNDEMIEIRRHLHENPELSFHEEKTAQYILDFY
                     KGKDVDVRSNVGNGHGIIVTIKGGKPGKTIGLRADFDALPILEETGLPFASKNEGVMH
                     ACGHDGHTAYLLVLADCLIQLKDQLPGTIKIIHQHAEEVPPGGAKSILESGELDDLDN
                     IFGIHLLPMSPAGVIGYHSGFSMAGRSYLKLGIQGVGGHGSSPHMANDAIVAGSYFVT
                     SIQTVISRRLNPFDMGVITIGSFDGKGTFNVIKDRVELEGDIRYMNKETQGVIEEEVR
                     RMVSGLEIKFDVTCDLTYASDYPPLYNDPEVTAAVKQSLENANDPHIKEVVEYPMMSP
                     SEDFAYYLEKIPGCFFFIGSKPKGVDKPYFNHHPKFDIDEDALIVAAKSVGYV"
BASE COUNT          715 a          550 c          615 g          647 t
ORIGIN      
        1 tcgatcatgg cgtttattcc acgacaaaaa ggcaagggga ttcaagcaac acaaccgcca
       61 ggccagccaa ccatctcaag ataaatgcac tccattaccg agccgcatca cggaggatgg
      121 cctgcagatc atttctaaaa ggagaccatg aacatgagag agaaattaat gcaattactg
      181 gaatcccgtg aggatgaaat gattcaaatt cgtcggtatt tacatgaaca tcccgagttg
      241 tcctttaaag aagaaaatac cgcccaatat attgcggatt tttacaaagg taaggatgtc
      301 gacgttcaaa ggaatgtcgg taatggatac gggatcatcg tgacgatcaa aggcggtaag
      361 ccagggaaga cgattgcgct tcgcgccgat tttgatgccc ttcctattct agaggagacg
      421 gatgtcccct ttaaatcaaa aaacgaaggc gtcatgcacg cttgcggtca cgacgcccat
      481 acagcctatt tactcattct tgccgattgt ctcattcaat taaaggactc gcttgccggc
      541 acaattaaaa tcattcatca gcacgcagag gaggcacctc ccggaggtgc gaaaagcatt
      601 gtagaatccg gtgtgcttga cgatgtggag aacattttcg gcattcacgt cttccctacc
      661 catccagcgg gatttgtcgg ctaccatagc ggctattcgc ttgccggaag atcctttttc
      721 aaactggtcg tccaaggtgt tggcggccat ggctcttccc cgcatatggc gaatgacgca
      781 attgtagctg ccgctcattt tgtcacagcc gtccaaacgg ttattagccg cagactcaat
      841 ccgttcgatc atggagtcat tacgattggc tcctttgatg gaaagggaac ttttaacgtc
      901 ataaaggata gtgtggaact tgaaggcgac gtacgctata tgacggttga aaatcagaag
      961 ttgattgcaa atgaagtgca gcggatcgtc aagggaattg aagttgaatt cgatgttcaa
     1021 tgtgaactga cttataccgc tgattatccg ccattgtaca acgatccgga agtgaccgaa
     1081 aaagtcgtag caagcttaaa aagcctggac gacccagaca tcaaagaagt tggcgaatat
     1141 ccgatgctgt caggttctga agacttcgca tactatctcg aaaagatacc tggctgcttc
     1201 ttttacattg gctgtaaacc aaaaggtgtg gaaactgctt atttcaatca tcatccgaaa
     1261 tttgacatcg atgaagattg ccttcttgtt gcagcgaaat ctgttgggca agtcgtctgc
     1321 agcttttacg agcaatagca gttctcggaa aggatgttac gaactatgaa aagtcaatta
     1381 atggagttat tggaatcccg taatgatgaa atgattgaaa tccgtcgtca tttacacgaa
     1441 aacccggagt tatccttcca tgaggaaaaa acggcgcaat atattttgga tttttacaaa
     1501 gggaaggacg ttgacgttcg ttccaatgtt gggaatggcc atggaatcat tgtgacgatc
     1561 aaaggaggaa aacctggtaa aacgattgga ctacgggcag attttgacgc cctgcctatc
     1621 ctggaagaga caggtctgcc gtttgcatcg aagaatgaag gggtcatgca cgcgtgcggc
     1681 catgatggcc atactgcata tttactcgtt cttgctgatt gtttgatcca gttaaaagac
     1741 caattgcccg gaacgatcaa aatcattcat cagcatgctg aagaagtacc gcctggcggt
     1801 gcgaaaagca ttttggaatc cggcgagctc gatgaccttg ataatatttt cgggattcat
     1861 ctgctcccga tgagcccggc tggtgtcatc ggatatcata gcggtttttc aatggcaggc
     1921 agatcatatt tgaaattggg cattcaaggg gttggcggcc atggctcatc cccgcatatg
     1981 gcaaatgatg caattgttgc cggctcctat tttgtcacat caatccaaac ggtcatcagc
     2041 cgccgtttga atccctttga catgggggtt ataacgattg gatctttcga cgggaagggt
     2101 accttcaatg tcataaaaga tcgtgtcgag ctagaaggcg atatccgtta catgaataag
     2161 gagacacaag gagtaattga ggaggaagtg cgccgcatgg tgagcggact cgagatcaag
     2221 tttgacgtta catgcgactt gacgtacgct tccgattatc cgccgctata caatgatcct
     2281 gaggtgacgg cagctgtaaa acaaagtctt gaaaatgcaa acgacccgca cattaaggaa
     2341 gtggtcgaat atccgatgat gtccccttcc gaagatttcg cttattattt ggaaaagatc
     2401 ccaggttgtt ttttctttat tggcagcaag ccaaaaggag tagacaaacc gtattttaac
     2461 catcatccga aattcgacat cgatgaagat gccctaatcg tcgccgcaaa atcagtcggt
     2521 tatgtcg
//
LOCUS       DASULI010000187         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1802363,
            whole genome shotgun sequence.
ACCESSION   DASULI010000187 DASULI010000000
VERSION     DASULI010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802363"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFX34_04050"
     CDS             <1..199
                     /locus_tag="VFX34_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5564110.1"
                     /translation="MLSMSPTALRFLKAAMNADTDGLAGLQQMAGDATLLYYTTEEAK
                     EGRDAFKEKRKPDFGQFPRFP"
     gene            274..1719
                     /locus_tag="VFX34_04055"
     CDS             274..1719
                     /locus_tag="VFX34_04055"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEX5564111.1"
                     /translation="MIPNWLLQRAYLTPNRMALSYGDKRWTFRQLSDEASLIARKLRW
                     NGLKEGDRIALLGRSTPEMVFIIHGCLLAGLEIVMLNSRLSSKEISWQLDDSEASTLL
                     VDDVFLDRINEISKPKLTFTAIESSGEENFAVTEMWERSRTITIMYTSGTTGFPKGVR
                     QTAGNHTSSALSSVLNLGLAEDDIWLCTMPLFHISGFSILARSVIYGMGVRLYEKFDA
                     ASIVEEIENGTATRISVVATGLQRILEQFEMNGSIAHPSFQTMLAGGGPVPDDYLRRA
                     QAYRLPVLQTYGMTETSSQTATLSSEDALRKSGSAGKPLFFNQIRIRDAEQPGDLGEV
                     LVMGPHVTPGYIGHASERDPLEDGWLPTGDIGYIDGEGYLFIVDRRSDLIISGGENIY
                     PAEIENVLVSHPQVKEAGVCGQDHPEWGSVPIAFIVGDGKLTASDLEGYCKQQLASYK
                     TPKAFHFVDELPRNASNKLLRRELKEWAKEK"
     gene            complement(1751..2716)
                     /locus_tag="VFX34_04060"
     CDS             complement(1751..2716)
                     /locus_tag="VFX34_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX5564112.1"
                     /translation="MKKKSFVLLALVLTLLIGACGNKEDKNKAATGNEGKLSVYTTVY
                     PLKYFTERIGGEFVDVKSIYPAGADEHSFDPTQKDMIALSDADLFFYIGLGLEGFVES
                     AQKTLKNEHVELVATAESISKAKLEEGQHEDEGEHEEHSHEHGQFDPHVWISPVLSID
                     LATSIKESLISKMPDQKEAFEKNFDLLEDDLMELDERFKEMAEKAETKTYFVSHAAFG
                     YLANEYGLNQVAIAGLNSQSEPSQKQLAKIVEQAKEENVQYILFEQNVSSKLTEVVRK
                     EIGAESLMLHNLGVLTQQDIQSDETYFTLMEHNLHVFEQALGVNK"
     gene            complement(2835..3050)
                     /gene="yidD"
                     /locus_tag="VFX34_04065"
     CDS             complement(2835..3050)
                     /gene="yidD"
                     /locus_tag="VFX34_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007201574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5564113.1"
                     /translation="MSIIKAYQKIISPLTPPSCRFYPTCSHYGVEALQKHGALKGAWL
                     TIRRILKCHPFHEGGFDPVPDKIDKKK"
BASE COUNT          798 a          670 c          753 g          830 t
ORIGIN      
        1 gatgctctcc atgagcccga ctgcactccg cttcctcaaa gcagcaatga atgctgatac
       61 ggacggacta gctggtcttc aacaaatggc tggggacgct actttgcttt attacacaac
      121 tgaagaagcg aaagagggac gcgacgcatt caaagagaaa cgcaaacctg acttcggtca
      181 attcccacgt ttcccttaat aatgtaaagt atagtaaaac attcaagctg cctgttcaac
      241 taggcagctt ttctattcga ggaaggtgag tatatgatac ctaattggtt gctccaaagg
      301 gcttatttga caccaaatcg gatggctctc tcttatggag acaagcgatg gacattccgg
      361 cagctttcag acgaagcctc tttaattgcc cggaaattgc gatggaatgg gttgaaagaa
      421 ggggatcgga ttgctttgtt gggacgatcg acacccgaga tggtctttat cattcatggc
      481 tgtctgttgg ctggactcga aatcgtcatg ttgaacagca ggctgtcttc gaaagagatc
      541 tcttggcaat tagatgatag tgaggcatcg actttactgg tggatgatgt attccttgac
      601 cgaatcaatg agataagtaa gccgaagctt acgtttactg caatcgaatc aagcggtgag
      661 gaaaattttg cggttactga gatgtgggaa cgttcaagga cgattacgat tatgtatacg
      721 tcagggacga ctggctttcc gaaaggcgtc cggcaaactg ccggcaatca tacatcaagt
      781 gcgttgtcct ccgtgttgaa tttaggtttg gccgaagatg atatttggct ttgcacaatg
      841 ccgctgttcc atattagcgg tttctccata ttggcaagat ccgtcattta tggaatggga
      901 gtacggctgt atgaaaaatt cgatgccgct tcaattgtag aggaaatcga gaatgggacg
      961 gcgactagaa tatctgttgt cgcgacaggc ttgcaacgga ttcttgagca atttgagatg
     1021 aacggatcga ttgcacatcc ttcatttcaa acgatgttag cgggaggagg cccagtgccg
     1081 gatgattatt tgcgtagagc gcaagcatac aggctccctg ttttgcagac gtacggaatg
     1141 acggaaacga gttcacagac agcaacgctt tcatccgaag atgcactgcg gaagtccggt
     1201 tccgcgggaa agcctttgtt cttcaatcag attcgaatca gggatgcaga acagccggga
     1261 gaccttgggg aagtccttgt aatggggcct cacgtgacac cggggtatat cggacatgca
     1321 tcggaaaggg atccgcttga ggacggatgg ctgccgaccg gggatatcgg ttatatcgac
     1381 ggggaaggct atttattcat tgtcgataga cggtccgacc tgatcatttc cggtggtgaa
     1441 aatatttatc ctgcagaaat cgaaaatgtg ctcgtttcgc atcctcaagt aaaagaggca
     1501 ggcgtttgcg ggcaagatca tcctgaatgg ggcagcgtac caatcgcatt catagtggga
     1561 gatgggaagc tgacagcatc ggatttggaa gggtattgca aacaacagct tgccagttac
     1621 aagacaccga aagcgttcca ttttgtagat gaattgccgc gaaatgcatc gaataaactc
     1681 ctgcgacgcg aactgaagga atgggcgaaa gaaaaataga gagggcttca tataagaacc
     1741 ctctctttgt tcatttattg acgccaaggg cctgttcgaa gacatgcagg ttatgttcca
     1801 ttaacgtgaa gtatgtttca tcgctttgga tatcctgctg cgtaaggact cccaggttat
     1861 ggagcattaa cgattctgcg ccaatttctt ttctgacgac ttccgtcagc ttcgaggaaa
     1921 cattctgttc gaatagaatg tattggacat tttcttcctt ggcctgctca acgatttttg
     1981 cgagttgctt ctgggaaggt tcgctttgcg aattcagtcc ggcaattgcc acttggttca
     2041 atccatattc atttgcaaga tagccgaatg cggcatgcga gacaaaatag gttttcgttt
     2101 ccgctttctc agccatttcc ttaaaccgct catccaattc cattaagtcg tcttcgagaa
     2161 ggtcgaaatt cttttcgaac gcctctttct gatcgggcat ttttgagatg agcgattcct
     2221 tgatggatgt cgccaggtca atgctcaaga caggggatat ccagacatga gggtcgaatt
     2281 gaccgtgttc atggctatgc tcttcatgct ctccctcgtc ctcatgctgt ccttcttcaa
     2341 gctttgcttt tgaaatggac tccgcggttg caaccaattc gacatgttcg ttctttaatg
     2401 tcttttgagc gctctccacg aatccttcca aaccgaggcc aatgtaaaag aacagatccg
     2461 catcggaaag ggcgatcatg tccttttgcg tcggatcgaa tgaatgctca tccgctccag
     2521 ccgggtagat ggatttgaca tcgacaaact cccctccaat ccgctcggtg aaatacttca
     2581 agggatagac tgtcgtatag acggacagct tcccttcatt gccggtcgcc gccttgtttt
     2641 tatcttcttt attcccacag gcaccgatga gcaaagtcaa gacgagtgcc agaagaacaa
     2701 atgatttctt cttcataagt acatcctcca ctagcttaat agtaatgatt acgatttaaa
     2761 cgctttctta taatacatgt taaaaacagt tttagcaact taaaagaaaa aaacggtcta
     2821 cgccggcgaa acttttattt tttcttatcg attttatcgg gaaccggatc gaagccgcct
     2881 tcatggaaag gatggcattt caagatccgt cgaatcgtca gccatgcgcc ttttaacgca
     2941 ccatgcttct gaagcgcctc gacgccataa tgcgagcaag tgggataaaa acggcaggaa
     3001 ggcggcgtga gcggagaaat gattttttga tatgctttta taatgctgat c
//
LOCUS       DASULI010000188         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1089892,
            whole genome shotgun sequence.
ACCESSION   DASULI010000188 DASULI010000000
VERSION     DASULI010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089892"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1839)
                     /gene="recN"
                     /locus_tag="VFX34_04070"
     CDS             complement(139..1839)
                     /gene="recN"
                     /locus_tag="VFX34_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX5564114.1"
                     /translation="MLSEISIHNFAIIEHLEVTFEEGLTVLTGETGAGKSIIIDAVQL
                     LAGARGSQEFIRHGAKKAELEGMFTIDDEHHPVFEKLSEFGIECEDGSIILRRDLNAN
                     GKTTCRVNGKLVTIASLREIGSQLIDIHGQHDNQELMHEKRHIHLLDQFAGSHLAEAH
                     SNYIDIYHQYTKLKRRLETANENEQQVAQRIDLYSFQLKEIDSAELVIGEEEELEEEK
                     LKLQHFNRIFERLNTAYESISGDNHALDWVGSAMSDLEDAASIDKDLESHSEIISTSF
                     YSLQDTAHELKSIIDRMEYDPTRLPIVEDRLAMIVSLKRKYGATIEDILLYREKIADD
                     LDQLLNRDERLGQEQEKLAQLEKDLEVEANELSIIRQEAAVRLEKAIMQQLKELHMEK
                     STFKVSINRKASNSYDSNGFDEIFFLISTNLGEPMKPLVKIASGGELSRIMLALKTIF
                     SKHQGVTSIIFDEVDTGVSGRVAQAIADKIAMIATHSQVLCISHLPQVAAMADHHLLI
                     MKEVKGNRTTTGVVEITELERTKELSRMLSGAEITSLTLQHADELLTMADERKTTFRV
                     "
     gene            complement(1873..2322)
                     /gene="argR"
                     /locus_tag="VFX34_04075"
     CDS             complement(1873..2322)
                     /gene="argR"
                     /locus_tag="VFX34_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HEX5564115.1"
                     /translation="MNKGQRHLRIRDIITNEEVETQDQLVERLKNAGVDVTQATVSRD
                     IKELHLIKVPLPDGRYKYSLPPVHKYNTEEKLRRMLTDAFVGIDSASHFIILKTLPGN
                     AHAVGSLIDNLGWNEMLGTICGDDTCMIICRDESLTVEVKDRLLALI"
     gene            complement(2363..3184)
                     /locus_tag="VFX34_04080"
     CDS             complement(2363..3184)
                     /locus_tag="VFX34_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX5564116.1"
                     /translation="MTNSQPKERVDVLLVQRGLVETREQAKRSIMAGIVFSAETRLDK
                     PGEKIKIDAPLSVKGAALKYVSRGGLKLEKALKVFDISVEDKIVLDIGSSTGGFTDCA
                     LQNGAKHCYALDVGTNQLAWKIRSDERVTVMEKTNFRHAEPGHFMKGTPQVATIDVSF
                     ISLTLIFPPLKRIITEGGDVVALVKPQFEAGKDKVGKKGIVKEKAVHLEVLRKIAESS
                     KLNGFSLRGISFSPVTGGEGNIEFLFHLQSEKDPVNPYDDSAFSALVAEAYETLT"
     gene            complement(3181..>3483)
                     /locus_tag="VFX34_04085"
     CDS             complement(3181..>3483)
                     /locus_tag="VFX34_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019380196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5564117.1"
                     /translation="NARFIKPLDTAMLDQLFNTGKPIVTVEEAALAGGFGSAVIEYAH
                     DVSSNVSISRIGIPDEFVEHGSVEKLLEEIDFTAENVVRTMELAVKGLTHAEREIV"
BASE COUNT          847 a          882 c          682 g         1072 t
ORIGIN      
        1 caaacgaagc ggataacatc agtcgtttac tccatcccat ggttcacctc ctcggctgaa
       61 agatactatt ggtatgtccg ttcaggtgaa tcttatgaaa ggttaaatca tgaaaaaacg
      121 catacatgat gtatgcgcct aaacacggaa agtcgttttc cgttcgtcag ccattgtcaa
      181 taattcatcg gcatgctgca atgtcagcga ggtgatttca gctcccgaca gcatccttga
      241 taattccttt gttctttcca attctgttat ttccacgact cctgtcgttg tacggttccc
      301 tttcacttct ttcatgatga gcaagtgatg gtcagccatt gccgcgactt gaggaagatg
      361 cgatatgcat agcacttgtg aatgcgtggc aatcattgcg attttatcgg caattgcttg
      421 ggcgacacgt ccgcttactc ccgtatcaac ttcatcgaaa atgattgacg ttacgccttg
      481 gtgcttggaa aagattgttt tcaacgccaa catgatcctc gacaactcgc ctccggatgc
      541 gattttcaca agcggcttca ttggttctcc gagatttgtt gaaatgagga agaaaatttc
      601 atcgaaccca ttactgtcat aactgttgga tgcttttcga ttaatggaaa ccttgaatgt
      661 cgacttctcc atatgcagct ctttcagttg ctgcataatc gccttctcta agcggaccgc
      721 cgcttcttgt ctgataatgg ataattcgtt cgcttccact tcgaggtctt tttcaagttg
      781 ggcaagtttt tcctgttctt ggccaagcct ctcatcccga ttgagcagtt ggtcaagatc
      841 gtctgcaatt ttctcccggt aaaggagaat atcttctatc gttgcgccat acttccgctt
      901 taacgataca atcattgcaa gtcgatcttc tacaattggc aatcgggtcg gatcatattc
      961 catacggtca atgatgcttt tcaattcatg tgcggtatct tgcaatgaat aaaagcttgt
     1021 tgaaatgatt tcggagtggg attccagatc cttgtcgatt gaagcggcat cttccaagtc
     1081 gctcattgca gatcctaccc agtccaatgc atggttgtct ccgctgatcg actcatatgc
     1141 agtgttcaag cgttcaaaga tcctattgaa atgttgaagt ttcagctttt cctcttcaag
     1201 ctcttcctct tccccaatga ccagctcagc actgtcaatt tctttcagtt ggaaagaata
     1261 gagatcgatc cgctgcgcaa cttgctgttc attctcgtta gccgtttcaa gtctgcgttt
     1321 caatttcgta tattgatgat aaatatcgat ataattactg tgcgcttcgg ccaaatgtga
     1381 acccgcaaac tgatcgagca gatggatatg ccgcttttca tgcatcaatt cctgattatc
     1441 atgttggcca tgaatatcta tcaattgcga gccgatttcc cttaaacttg caattgtcac
     1501 tagcttgcca tttacccggc aagtcgtttt gccatttgca ttcaaatcac gtcgtaagat
     1561 gatactgcca tcctcgcatt caatgccgaa ttcagagagt ttttcaaata cgggatggtg
     1621 ctcgtcatcg attgtgaaca tcccttccaa ttcagctttt tttgccccat ggcgaatgaa
     1681 ttcctgcgag cccctggccc cggcaagaag ctgaaccgca tcaatgatta tggactttcc
     1741 ggcacctgtt tcacctgtca ggacagtcag tccctcctca aaagtcactt cgagatgctc
     1801 tatgatggca aaattatgaa tggaaatttc acttaacatt caatcatgca cctccttcat
     1861 cgaacgttca gcttatataa gcgctaacag cctatctttc acttcgaccg ttaaagactc
     1921 atccctgcaa atgatcatgc atgtatcgtc tccgcaaatc gtccctagca tttcattcca
     1981 ccctaaatta tcgatgaggg aaccgactgc atgcgcattt ccaggcaatg tcttcagtat
     2041 gatgaaatga ctggcactat ctatacctac gaatgcatcc gtcaacatcc gacgaagctt
     2101 ttcttccgta ttgtatttat ggacaggcgg caggctatat ttataacgtc catcaggcaa
     2161 tgggactttg ataagatgca attctttaat gtcgcgggat actgttgctt gtgtcacgtc
     2221 gactcctgcg ttttttaatc tttcaacaag ctgatcctga gtttccactt cctcgtttgt
     2281 aatgatatcc cggatacgca gatgccgttg tcctttattc aatacaatcc ctcccatact
     2341 tattcacacc tttcttttgg aatcatgtca acgtttcgta agcttcagcc accaatgcac
     2401 tgaaagctga atcatcataa ggattgaccg ggtctttctc cgattgcaaa tggaatagga
     2461 actcgatatt cccttccccg ccagtcactg gagaaaagga aatgccccgt aaagaaaatc
     2521 cgttcaattt ggaggattcc gctatcttac gcaaaacttc caagtggact gccttctcct
     2581 tcacaatccc ctttttcccc actttatctt ttcctgcctc gaattgtggt ttgacaaggg
     2641 caacaacatc tccgccttct gttatgattc tcttcagtgg cggaaaaatc aatgtaagcg
     2701 atatgaaaga gacgtcaatt gtggcaactt gtggcgtccc tttcatgaag tgcccgggtt
     2761 ccgcatgcct gaagttggtt ttctccatga cggtcacccg ctcatcgctt cgtattttcc
     2821 acgcaagctg gtttgttccg acatcaagtg cataacaatg ttttgcgccg ttttgcaatg
     2881 cgcaatccgt aaagccgccc gtagacgaac caatgtccaa aacaatttta tcttcaaccg
     2941 atatatcgaa gacctttagg gctttttcaa gtttcaatcc gccacggctc acgtatttca
     3001 atgctgcccc tttaacggac agcggcgcgt ctatcttgat cttttcgccc ggcttgtcca
     3061 atcttgtttc tgcagagaac acaattcccg ccatgatgga acgtttcgcc tgttctctcg
     3121 tctccactag ccctctttgg acaagtagta catcaacgcg ttcttttggt tgggagttcg
     3181 tcatacgatt tccctttcag catgcgtcaa tccttttaca gccagttcca tcgtccttac
     3241 gacattctcc gcagtaaaat cgatttcctc gagcaatttt tcgacactgc catgttcgac
     3301 gaattcatca gggataccta ttcggctaat ggaaacattt gaagagacat catgcgcgta
     3361 ttcaatgact gcactgccaa acccgcctgc caatgctgct tcttcaactg tgacaatcgg
     3421 tttgccggta ttgaaaagct gatccagcat agcggtatcc aaaggcttga taaaccttgc
     3481 gtt
//
LOCUS       DASULI010000189         3562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1718885,
            whole genome shotgun sequence.
ACCESSION   DASULI010000189 DASULI010000000
VERSION     DASULI010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718885"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFX34_04090"
     CDS             <1..426
                     /locus_tag="VFX34_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564118.1"
                     /translation="VVETKSVPEGLAAILAFNPEAGVAENEKVMSKAAAGVKTGQVTH
                     SIRDTSIDGIDIKKDDFMGLAGSKIVISNPSLDEVMKTLLESLIDEDDEIVTIIYGED
                     VTEQEANEIAEYVESTYDHTEVELYNGKQPLYPYILSVE"
     gene            542..1204
                     /gene="sdaAB"
                     /locus_tag="VFX34_04095"
     CDS             542..1204
                     /gene="sdaAB"
                     /locus_tag="VFX34_04095"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEX5564119.1"
                     /translation="MKYRSVFEIIGPVMIGPSSSHTAGAARIGRVARDLFGRQPEWAR
                     IHLYGSFAETYKGHGTDVAIIGGLLDYDTFDERIKTAFEDAAAAGMTFEFIPEEEEAD
                     HPNTAKIVIGDSKGEMELVGISIGGGTMEVTELNGFPLRLSGHFPALLVVHDDRAGVI
                     ANVSNAIASQGMNIAHMEVGRKEKGKMALMVIEVDQMFDETLINELKSLPNVTQVSTL
                     AN"
     gene            1219..2127
                     /gene="sdaAA"
                     /locus_tag="VFX34_04100"
     CDS             1219..2127
                     /gene="sdaAA"
                     /locus_tag="VFX34_04100"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEX5564120.1"
                     /translation="MEVLFGTVKELVALAEAQNKPISEIMIEQEMQITRRSREEIVAQ
                     MENNLSVMESAVEQGLQGVKSTSGLTGGDAVLIQNYIKSGKSLSGDLVLDAVSKAVAT
                     NEVNAAMGMICATPTAGAAGIVPGTLFAVKDKLNPTREQMVNYLFTSGAFGFVVANNA
                     SISGAAGGCQAETGSAASMAAAAIVEMAGGTPQQSAEAFSIVMKNMLGLVCDPVAGLV
                     EVPCVKRNAMGAAKALVGADMALAGVVSRIPTDEVIEAMHRIGQSMPSALRETAKGGL
                     AATPTGKALEAEIFGKDKAVSDTVNS"
     gene            2108..>3562
                     /locus_tag="VFX34_04105"
     CDS             2108..>3562
                     /locus_tag="VFX34_04105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5564121.1"
                     /translation="MTPSILDSVTTLKGVGKASAEQLQKLGIETIEQLINTFPYRHED
                     FRLKNLAETPHNERVTIEGRVESEPSVLFLGRNKSRLQIRLLAGNHLVKAVFFNQHYL
                     KDRLSIGMVVTVTGKWDRGRQVINVSNFNEGPKTDQADFEPVYSLKNVMYQKTFRKYM
                     RQALDQYKDEIVEVLPLSLRESYMLPSIGEALEMVHFPKSPNDVKQARRRFVYEELLK
                     FQLKMIGMKKIRKENEKGTPIHYDLERLKEFIASLPFELTNAQKKVVNELCAELKSPV
                     RMNRLLQGDVGSGKTVVAAIALFAAITGGFQGALMAPTEILAEQHADSLSAWLEPFGV
                     RVAFLSGSTKSKSRRIIIEKLQTGEIDLLIGTHALIQPDVGFAKLGLVITDEQHRFGV
                     EQRRVLREKGLNPDVLFMTATPIPRTLAITAFGEMDVSILDEMPAGRKKVETYWLKEE
                     SLLPVLRKMEKELQAGRQSYVICPLIEESDKLDVQ"
BASE COUNT         1038 a          738 c          938 g          848 t
ORIGIN      
        1 gtcgttgaaa cgaaatccgt tcccgaagga ttggctgcaa ttctcgcatt caatcccgaa
       61 gctggcgtcg cagaaaatga aaaagtgatg tcgaaagcag cagctggcgt gaagaccgga
      121 caagtcacac attcgatccg ggacacgtcc atcgatggca tcgacattaa aaaagatgat
      181 ttcatggggc ttgcgggcag taaaatcgtg atttcaaacc catctctgga tgaagtgatg
      241 aaaacattac ttgaatcact tattgatgaa gatgatgaaa ttgtcacgat catttacggc
      301 gaggacgtaa cggagcaaga agctaatgaa attgcagagt atgtggaatc cacatacgat
      361 catacggaag tcgaattata caacggaaaa caaccgctgt atccttatat cctatccgtg
      421 gaatgatacg gaaaaacgac ggcacgcctt ttcggggcgt gccgattttc tattttgtct
      481 ttaattgaaa tctacggtaa aatgaaagaa gtgtttgctt cttaagaagg gggtaatttg
      541 tttgaaatac agatctgttt ttgaaattat cggtcccgtt atgatcgggc cttcatcttc
      601 acataccgca ggagctgccc ggataggaag agtcgcccgg gatttgttcg gtcgtcagcc
      661 ggagtgggca agaatccatc tctatggttc tttcgcagaa acctataaag ggcatggaac
      721 ggacgttgcg attatcgggg gattattgga ttacgacacg tttgatgaga gaataaaaac
      781 agctttcgag gatgcggctg cagccggcat gaccttcgag ttcattcccg aagaggaaga
      841 agctgatcat ccgaatacag caaaaatcgt cattggtgac agtaaagggg aaatggaact
      901 tgtcggtatt tcaatcgggg gcggtacgat ggaagtcact gagttgaacg gttttccgct
      961 tcgcctctct ggacatttcc cggcgcttct tgttgtacat gacgatcgcg cgggggtcat
     1021 tgcgaatgtt tccaacgcca tcgcttcaca aggcatgaat atcgcccata tggaagtcgg
     1081 caggaaagaa aaaggtaaaa tggcactgat ggttattgaa gtcgaccaga tgtttgatga
     1141 aacactcata aatgaattga aatcgttgcc gaatgtcacg caagtttcaa cattggcaaa
     1201 ctaaggaggg aaatagcaat ggaagttcta ttcggtacag taaaggaact agtggcactc
     1261 gcagaagcgc aaaataagcc gatttcggaa attatgatcg aacaggaaat gcaaattacg
     1321 agacgttcaa gggaagagat tgtcgctcaa atggaaaata atctttccgt catggaaagc
     1381 gcagttgaac aaggactgca aggcgtgaaa tccacttcag gtttgactgg aggcgatgct
     1441 gtccttatac aaaactatat caagtcgggg aaatccttat ccggagacct tgttttggat
     1501 gctgtcagta aagcggtcgc tacgaatgaa gtgaatgcgg caatggggat gatctgcgcc
     1561 actcctacag caggtgctgc cgggatcgta ccaggaacgc ttttcgcggt caaagacaaa
     1621 ttgaacccga ccagggaaca gatggtgaat tacctgttca cttcgggtgc attcggcttt
     1681 gtcgtcgcaa ataatgcctc gatctcggga gctgcaggcg gatgccaggc agaaacgggt
     1741 tcagccgctt cgatggctgc agccgcaatt gttgaaatgg ccggtgggac acctcagcag
     1801 agcgcggagg ctttttcaat cgttatgaaa aacatgcttg gacttgtttg tgatccagta
     1861 gcaggccttg tggaagtgcc atgtgtgaag cggaatgcaa tgggagccgc aaaagcgctt
     1921 gtcggggctg acatggctct tgcaggagtc gtcagcagga ttccgacgga tgaagtaatt
     1981 gaagcgatgc atagaatcgg acaatcaatg ccttccgcac tcagggaaac cgccaaaggg
     2041 ggcttagccg ccactccgac cggaaaggcg ctggaagcag agatattcgg aaaggataaa
     2101 gcggtaagtg acaccgtcaa ttcttgattc tgtcacaacg ctgaaaggtg ttggcaaagc
     2161 ctctgctgaa caacttcaaa aactgggcat tgaaacgatt gaacaactga ttaatacatt
     2221 tccgtaccgc catgaagatt tcagattgaa gaatttggcc gaaacgccgc ataatgaacg
     2281 ggtcaccatt gaaggaaggg tcgaaagcga gccttccgtt ctttttttag gcagaaataa
     2341 atcacgcctt caaattcggt tgctggctgg caatcatctc gtcaaagcgg ttttcttcaa
     2401 ccaacattat ttgaaggacc gcctttccat cgggatggtt gtaacagtga ccggaaaatg
     2461 ggatcgcggc aggcaagtga tcaacgtaag caacttcaac gaaggaccga aaacggatca
     2521 agccgatttc gaaccagtct atagtttgaa aaatgtcatg taccagaaga cgttccgaaa
     2581 atatatgaga caagcacttg atcagtacaa ggatgaaatc gtcgaagttc ttccgctttc
     2641 attgcgggaa tcctatatgt tgccatcaat aggagaagca cttgaaatgg tgcatttccc
     2701 taaatcgccg aatgatgtga aacaagcacg aagacgcttt gtctatgagg agttattgaa
     2761 atttcagcta aaaatgatag gaatgaaaaa aatccggaaa gagaacgaaa aaggaactcc
     2821 aattcattat gatctcgaac ggttaaagga gtttatcgct tccttgccat ttgaactgac
     2881 aaatgcgcag aaaaaagttg tgaatgaact ttgtgctgaa ctgaaaagtc cagtaagaat
     2941 gaaccgtctt ctgcaagggg atgttggttc cggtaaaacg gtcgtagcgg caatcgccct
     3001 atttgcagcg atcactggag ggtttcaagg tgcgctcatg gcgccgaccg aaattcttgc
     3061 agagcagcat gccgattcgc tttctgcatg gcttgagccg tttggggtga gagtggcttt
     3121 tctctctggg tccacgaaaa gtaaatccag aaggatcatc atagagaaat tacagaccgg
     3181 agaaatcgat ctgctcatcg gaacgcatgc actcattcag ccagacgtcg gctttgcgaa
     3241 attaggactt gtcataacgg atgaacagca tcggtttggt gttgaacaac gccgcgtgct
     3301 ccgtgaaaag ggactgaacc cggacgtcct attcatgacg gcgactccga ttccccggac
     3361 gttggcgatc actgccttcg gagaaatgga tgtgtccatc cttgatgaaa tgccggcggg
     3421 cagaaaaaaa gtcgagacgt attggttaaa agaggaatcc ttattgcctg tgctgcgaaa
     3481 aatggaaaag gagctgcagg ccgggagaca atcatatgtc atttgcccgt tgatcgagga
     3541 atcggacaag ctcgatgtgc aa
//
LOCUS       DASULI010000190         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1699344,
            whole genome shotgun sequence.
ACCESSION   DASULI010000190 DASULI010000000
VERSION     DASULI010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699344"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1081)
                     /locus_tag="VFX34_04110"
     CDS             complement(110..1081)
                     /locus_tag="VFX34_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5564122.1"
                     /translation="MTVKWIGKRMMLGILVLFIVSFLSFFIMHAGPGSSATAYYGGNA
                     QTLTTAEKERISKAFALDRPLIVQYGAWLKETVQGNLGMSAKEGRPVASILGERLPNT
                     LMLFGASMFFIVLGSIWLGMAAGMKPGSLLDRGLSSFSIASSSIPAFWLGILFIYLFA
                     VTLGVLPSSGTRSLGGDGGFVDKMRHLIMPASVVVLTHVGLYARFLQESVKAEIGSYY
                     VMAARANGVDEREIRRGVLRNAFIPYLNYLGMTIPSFFGGSVIVEALFSWSGLGQLLV
                     KSVMVQDYPLLMGGIMLTGLIVVVSLFVIDVLMYMLDPKLRKKELVG"
     gene            complement(1071..>2356)
                     /locus_tag="VFX34_04115"
     CDS             complement(1071..>2356)
                     /locus_tag="VFX34_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5564123.1"
                     /translation="DIRFHDGEPLTAEDVIFTIESVLDDKNASFLKSDFLEVESLKKI
                     DDYEFELKLKHPFTPILDKLTLPILPKHAFEGVDMRTAEFNTHPIGAGPYQFDRWDRG
                     NSLTLKAYEQFHGTKPSIDKVIFKFIPDSNVRALQLASGEVDIALLDPVQVGELEKRD
                     HVKIYDIDTADYRGILFNMQNDLWQDVRVRQAFSYAIDRAQIVKGILKGFGEEAYSPL
                     QKHAYHNENIEKYTYDAAHAEALLDEAGWEKSDDGFRYKDGKKLSFTITAPASDAVRV
                     NMANYVAEGFKSIGADVTVAALDWSAITIEDTDAFMIGWGSPYDADHHTYILFHSAES
                     SLTGSGYNYGNYTNEKVDALLEKGRLSMDEQERKASYMAFQEELANDPAFAFIAYVNA
                     VYGIHGNIEGVKERTLGHHGSGFLWNVEEWKWNDR"
BASE COUNT          613 a          672 c          510 g          561 t
ORIGIN      
        1 tccaaatcca tttcattcac gggctgtgtt gccaaaagat ccgagaacaa tgtcaaggcg
       61 aggatcaggc cgagaagcga gcaccaaaat accagtttcg tccccgattt catccgacca
      121 actccttttt ccggagcttc ggatcgagca tatacatgag gacgtcaatg acaaacaggc
      181 tgacgacgac aatcaagcct gtcagcatga tgccgcccat aagcaatgga taatcctgca
      241 ccatgacgga tttcacgagc aattgcccaa ggcccgacca tgaaaacaaa gcttccacga
      301 tgacggagcc gccgaaaaac gatgggatcg tcatcccgag ataattcaaa taagggatga
      361 aggcgttccg taagacacct cgcctgattt cccgctcgtc caccccgttc gccctcgccg
      421 ccatcacata atagctcccg atttcggctt ttacgctttc ctgaaggaag cgcgcataca
      481 gtccgacatg cgtcagcacg acgacggatg ccggcatgat aaggtgcctc attttatcga
      541 caaaaccgcc gtccccgcca aggcttctcg ttccggacga cggcaagacg ccaagtgtga
      601 cggcaaatag ataaatgaat aaaattccaa gccaaaatgc cgggatggaa gatgaggcga
      661 tgctgaatga agataagccc ctatctagca gcgaccccgg tttcattccc gccgccatcc
      721 cgagccaaat cgaaccaagg acaatgaaaa acatcgaggc accaaacagc attaacgtat
      781 tcggcagccg ctccccaaga atagaagcga caggacggcc ttccttggcg gacatgccaa
      841 gattcccttg cactgtctcc ttcagccacg ccccatattg gacaatcaaa ggccgatcca
      901 gcgcaaacgc cttgctgatc cgctcttttt ctgcggtggt caatgtttgg gcattgcctc
      961 cgtaatatgc cgtcgcggag cttcccggcc ctgcgtgcat aatgaaaaaa gagaggaagc
     1021 tcacgatgaa gaggactaga atccccaaca tcatgcgctt cccaatccat ttaacggtca
     1081 ttccacttcc actcctcgac gttccaaagg aaaccggagc cgtgatggcc aagcgttcgc
     1141 tccttcacgc cttcgatatt tccgtgtatg ccatagaccg cattgacata agcgataaat
     1201 gcgaatgccg gatcgttcgc caattcttcc tggaaggcca tatagcttgc cttcctttcc
     1261 tgctcatcca tcgacaaacg tcctttttca agcaatgcat ccactttttc attcgtataa
     1321 tttccgtaat tataaccaga tcccgtcaaa ctcgattccg ccgagtggaa taagatgtac
     1381 gtatgatgat ccgcgtcata cgggctgccc caaccaatca tgaacgcatc cgtatcttca
     1441 atcgtgatgg cactccagtc aagcgctgca acagtgacat ccgcaccgat tgacttgaag
     1501 ccttccgcga catagttcgc catattgact cgcactgcgt ccgatgcagg cgccgtaatt
     1561 gtgaagctca gcttcttgcc gtccttataa cggaaaccat catctgactt ttcccagcct
     1621 gcttcatcta gcaaagcctc agcatgagca gcgtcatacg tgtacttttc aatgttttca
     1681 ttatgataag catgcttctg aagcggcgaa taggcctctt ccccgaagcc tttcaaaatg
     1741 cctttcacaa tttgcgcccg gtcgatggca tagctgaatg cttgccgcac ccgtacatct
     1801 tgccaaaggt cattttgcat attgaaaagg attccgcggt agtccgcagt atcgatatcg
     1861 tatattttca catggtcgcg cttctccaac tcaccgactt gcactggatc gagcagagca
     1921 atatcaactt cccctgaagc caattgcaat gcccgcacat tgctgtcggg aatgaatttg
     1981 aagatgactt tatcaattga tggcttcgtg ccatggaact gctcatacgc cttcaacgtt
     2041 aagctattcc cgcggtccca tcggtcgaac tgataaggtc ctgcaccaat cgggtgggta
     2101 ttgaattccg ccgtccgcat atccacgcct tcaaatgcat gcttcggcaa aatcggcagt
     2161 gtcagcttat ccaaaatcgg cgtgaaagga tgcttcagct tcagttcgaa ttcatagtca
     2221 tcaatctttt tcaaagattc cacttcaagg aagtcggatt tcaggaacga ggcattcttg
     2281 tcatctaata cactttcaat tgtaaagatg acatcctccg cagtcagcgg ttcaccatcg
     2341 tggaaccgga tgtctt
//
LOCUS       DASULI010000191         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_109768, whole
            genome shotgun sequence.
ACCESSION   DASULI010000191 DASULI010000000
VERSION     DASULI010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109768"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1281
                     /locus_tag="VFX34_04120"
     CDS             4..1281
                     /locus_tag="VFX34_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5564124.1"
                     /translation="MEKKQLNRVLVASLVGSSIEWFDYFLYGTVAALVFNQLFFVNED
                     PTIGLLLAYASFALAFFIRPFGGIIFSHIGDRIGRKKTLVLTLSLMGIATFGMGLLPT
                     YQAIGIWAPILLITLRLIQGLGIGGEWGGALLLAVEYAPPEKRGLFGSVPQMGITIGM
                     MLGTFALSIMTMLPEGSFMTWGWRVPFILSALLVIFGLWIRKGIDETPSFKEVQERGE
                     IPKIPLVTTFKTHWREVLIAIGAKVVETAPFYIFSTFIVSYATSYLDYSRTVTLNAVM
                     IATIVTTILIPIMGNLSDKVGRKPLYVWGSVAMILFAFPYFWMIKQGSVTMLVIATII
                     GLGIIWAPITAVLGTMFSEIFSAEVRYTGISLGYQIGAAVAGGTAPLVATALLVEFNN
                     SYVPVALYIIFTAVVSLIAIWAVKDRKNEALDQ"
     gene            1300..2289
                     /locus_tag="VFX34_04125"
     CDS             1300..2289
                     /locus_tag="VFX34_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5564125.1"
                     /translation="MLVINEREIQATYGMKEALHDVEQILRAKAEDKIANPHRTVIEF
                     PQHSASVLYMPSADSVNEIATIKTVTIFPENPAMGKPTTQGVILVSDAKNGEHLAMMN
                     GSYLTRLRTGALSGIATDRLARKDATVLTVIGTGAMAFEQVLGVLEVRPISKMLLVNP
                     TTAKAERFKERLLAFGIDENIQIELMDNVSEAVSQADIICCSTRSNTPVFDGNDVKPG
                     THVNGVGSYLPHMREMDFTFIQRCSKIVTDDEAGVKEEAGELIHANEQPEWSFDDLHG
                     ELMDVVSGVAAGRADDEEITFFKSVGAAYFDLAVAQGVYRKALEAGIGIEVEV"
     gene            complement(2457..>3469)
                     /locus_tag="VFX34_04130"
     CDS             complement(2457..>3469)
                     /locus_tag="VFX34_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5564126.1"
                     /translation="KSKLAPTLLIVGLLLALAGCVGPLEDEEESTEKATTEQVSTDEK
                     ESLEEKESTQEEKAPAEDTSKVEKEETEKNETKSEAEKTTPVVGAPSAEKKPATTAKP
                     APVKKTETSGTTAQVPVKLVKTIDGDTIKVLYNGKEMNVRYLLIDTPETNHPRLGKQP
                     FGEEAKERNRQLVNSGALTLEFDIGERLDKYDRLLAYVYVDGKSVQKTLLAEGLARVA
                     YVYPPNTRHLTPFEEAQAEAKKKGLGIWSVENYATDSGFNSDAAPKTSSSGSTAVTPK
                     APSTTASKPAATPSTNVAGGQEWFQNCTELRKKYPSGVPQGHAAYQAKMDRDKDGYAC
                     ER"
BASE COUNT          806 a          851 c          884 g          928 t
ORIGIN      
        1 aatgtggaga agaaacaatt aaatagagtg ttggttgcaa gtctcgtcgg aagttcaatc
       61 gaatggttcg actatttcct atatggaaca gttgctgcac ttgttttcaa ccaactcttt
      121 tttgtcaatg aagacccgac aatcggcctg ttattggcgt atgcgtcctt cgcgctcgcc
      181 tttttcatcc ggccattcgg cggcatcatt ttcagtcata tcggagaccg gatcggaagg
      241 aagaaaacgc tcgttttgac attaagcctt atggggattg cgacattcgg gatggggtta
      301 ttgccgactt accaggcaat cggcatctgg gcgccgattc tgttaattac actacgactc
      361 atccaaggtt taggaatcgg cggcgaatgg ggtggagcac ttttactcgc agtcgaatat
      421 gcgccaccgg aaaagcgcgg tcttttcgga tccgttccgc aaatggggat tacaatcggg
      481 atgatgcttg gaacgtttgc attgtcgatc atgacgatgc tgccagaagg ttcattcatg
      541 acatggggct ggcgcgtgcc gttcatctta agtgcattac ttgtcatctt cgggctgtgg
      601 attcgtaaag ggatcgacga aacgccttca ttcaaagagg tccaggaacg gggagagatt
      661 ccgaaaatcc ctctcgtcac aacattcaaa acgcattggc gtgaagtgct gatcgcaata
      721 ggagcgaaag tcgtcgaaac ggcgccgttc tacatattca gtacattcat cgtttcctat
      781 gcaaccagtt atcttgacta ctcgcggact gttacattga atgctgtaat gattgcgaca
      841 atcgtcacca cgattttaat tcccatcatg gggaatctat cagacaaagt cggacgcaaa
      901 ccgctgtatg tatggggctc ggtcgcgatg atcctattcg ctttcccgta cttctggatg
      961 attaagcaag gatctgtcac aatgctagtc atcgcgacaa tcatcggact cggcatcatt
     1021 tgggcgccga tcactgcggt tcttgggacg atgttctctg aaatcttctc ggcggaagtc
     1081 cgctacacag gcatttcgct cggctatcaa atcggtgcag cggttgcagg aggtacagca
     1141 cctcttgtcg ctactgcgct gcttgtcgaa ttcaataatt catatgttcc ggttgcgctt
     1201 tatatcattt tcaccgcggt tgtttcgcta atcgccatct gggcagtgaa agaccgaaag
     1261 aacgaagcgc tcgatcaata agttgaaggg gcgatttcaa tgttagttat caatgaaaga
     1321 gaaattcaag caacgtacgg aatgaaagag gcgttacatg acgtcgaaca gattttacgc
     1381 gcgaaagcgg aagataaaat tgccaatcca catcggaccg tcatcgaatt ccctcagcat
     1441 agcgcatccg tgctctatat gccgagcgct gattcagtga atgaaatagc gacgatcaag
     1501 acggtgacga tttttccgga aaatccggcg atggggaaac cgacgacaca gggcgtcatt
     1561 ctggtatcgg acgcaaagaa cggcgaacat ctggcgatga tgaacggttc ctatttgacg
     1621 aggcttcgga ccggagcgtt aagcggaatc gcaaccgacc gtcttgcgcg gaaagatgcc
     1681 actgtattga ctgtcatcgg aaccggagcg atggcatttg aacaagttct cggcgtattg
     1741 gaagtccgcc cgatctcaaa aatgctgctt gtcaatccga caaccgccaa ggcggaacgt
     1801 ttcaaagaga gactgctcgc gttcggcatc gatgaaaaca ttcagatcga actaatggat
     1861 aatgtgtcgg aagcggtcag ccaggcggat atcatctgct gcagcacccg ttcgaatacg
     1921 ccggtatttg acgggaatga tgtaaagccc ggcacacatg tgaatggcgt cggctcctac
     1981 ttgccccata tgcgtgaaat ggatttcacc ttcattcaac ggtgcagcaa aattgtcacg
     2041 gacgacgagg ccggtgtgaa agaggaagcc ggagagttga tccacgccaa tgagcagcct
     2101 gaatggtctt ttgatgattt gcatggcgag ctgatggacg tggtgtccgg tgttgctgcg
     2161 ggtagggcag acgacgagga aatcaccttc ttcaagtcgg tcggtgcggc gtacttcgat
     2221 cttgctgtag cccaaggtgt ttaccggaaa gcgttggaag caggaattgg gattgaagtg
     2281 gaagtataat gaagcgtaat aaagcggatt gccataacaa caggcaatcc gcttttacgt
     2341 attggaaggt ttaatgtctt tcattgaatc gcgtccaaag cagcgtgctg cgcgtccgat
     2401 gcatgccacc tcaaataaaa aaccggaaga ggggagtcct cttccggttc aaacgattat
     2461 ctctcacacg catatccatc tttgtctcgg tccattttag cctggtaggc agcatggcct
     2521 tgtggaacac cgctcggata tttctttcgg agctccgtgc agttttggaa ccattcctgt
     2581 cctcctgcaa cattcgtgct cggtgtcgct gccggtttgc ttgcagttgt gctcggcgct
     2641 ttcggcgtca cagctgtact gcctgaactt gacgttttcg gcgctgcatc gctattgaat
     2701 cccgaatcgg tcgcataatt ttcgaccgac cagatgccga gccctttttt cttcgcttcc
     2761 gcttgcgctt cctcgaatgg cgtcagatgg cgggtattcg gcggatatac gtaagctaca
     2821 cgggccaacc cttccgcaag caacgtcttt tggacgcttt tcccgtcgac atatacgtaa
     2881 gcgagcagtc gatcgtattt atccaaacgt tccccgatat cgaattcgag cgtaagcgcg
     2941 ccgctattca ccaattgccg gttgcgctcc ttcgcctctt ctccgaacgg ctgcttgccg
     3001 agccgcggat gattcgtttc cggcgtatcg atcaataaat aacggacatt catctctttt
     3061 ccgttataca aaactttgat tgtatcgccg tctatcgtct tcacgagttt gacgggaact
     3121 tgcgccgtcg taccggacgt ctcggtcttc ttcaccggag ccggtttcgc ggtagtagcg
     3181 ggctttttct ctgccgaagg ggctccgact actggagtgg ttttttcggc ttccgatttc
     3241 gtttcgttct tttcggtttc ttccttttca actttagaag tatcttcagc aggcgctttc
     3301 tcttcctgcg tagattcctt ctcttccaat gattccttct cgtctgtaga aacctgctca
     3361 gtcgtcgctt tttcagtaga ctcttcttca tcttctaatg gaccgacaca tcctgcgagt
     3421 gcaagcaata atccgacgat caataaagta ggagccaatt tcgacttcc
//
LOCUS       DASULI010000192         4513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_819645, whole
            genome shotgun sequence.
ACCESSION   DASULI010000192 DASULI010000000
VERSION     DASULI010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819645"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFX34_04135"
     CDS             complement(<1..640)
                     /locus_tag="VFX34_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002175733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX5564127.1"
                     /translation="MTKKPIYFNHDGGVDDLISLFLLLRMDNIELTGVSVIPADCYLE
                     PAVYASRKIIDRFGNGVNLDVAESNSRGKNPFPKDWRMHAFLVDALPILNESGKIDTP
                     VADKPAHLHMIDAIRNTSEKTTLLFTGPLTDLARALDEAPDIEEKIERLVWMGGTFRE
                     DGNVHEPEHDGTAEWNVFWDPEAAHRVWETSIEIDLVALESTNQVPLTLDVRE"
     regulatory      complement(799..900)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(1097..1489)
                     /gene="rpsI"
                     /locus_tag="VFX34_04140"
     CDS             complement(1097..1489)
                     /gene="rpsI"
                     /locus_tag="VFX34_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002009728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5564128.1"
                     /translation="MAQVQYIGTGRRKSSVARVRLVPGEGKIVVNNRDVEDYVPFETL
                     REVIKQPLVATQTLGSYDIHVNVHGGGYTGQAGAIRHGVARALLTVDPDFRGTLKSAG
                     LLTRDSRMKERKKYGLKGARRAPQFSKR"
     gene            complement(1509..1946)
                     /gene="rplM"
                     /locus_tag="VFX34_04145"
     CDS             complement(1509..1946)
                     /gene="rplM"
                     /locus_tag="VFX34_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020063210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5564129.1"
                     /translation="MRTTFMAKGHEVERKWLVVDAEGQTLGRLASEVAAILRGKNKPT
                     FTPHVDTGDHVIIINAEKIQLSGNKLKDKIYYRHTGYTGNMKQRTALEMRTNYPTKML
                     ELAIKGMLPKGPLGRQTIKKLHVYAGPEHPHTAQKPEAYELRG"
     gene            complement(2122..2862)
                     /gene="truA"
                     /locus_tag="VFX34_04150"
     CDS             complement(2122..2862)
                     /gene="truA"
                     /locus_tag="VFX34_04150"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX5564130.1"
                     /translation="MRRVKATVAYDGTGYAGYQFQPNMRTVQSVIDKALVKLHKDSSI
                     FSVASGRTDAGVHAWGQVIHFDTPLELPLDRWQMALNVLLPKDVRIVKTEYVDDDFHA
                     RYSATGKTYMFKWTSSEVQSPFERNYSVHLGKWRPDIERMCEAAKYIIGTHDFTSFCS
                     SKTATENKVRTVRELTVERRGEELIMTIEGDGFLYNMVRTIAGMLFAVGIGWNEPQDM
                     KEILEAKDRKKAGKTAPAHGLYLMEVTY"
     gene            complement(2864..3667)
                     /locus_tag="VFX34_04155"
     CDS             complement(2864..3667)
                     /locus_tag="VFX34_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEX5564131.1"
                     /translation="MLEKMIFGRYVPGDSFVHRLDPRSKLLFVFMFIVAVFLANNTVT
                     YTLLLAFTLFVISIARIRLYFLFNGLKPILFLIIFTLLMHFIFTKEGALLVDWKFIKI
                     YEEGVRQGIYISIRFLVLVLMTTILTLTTSPISITDGMETLLNPLKRIKLPVHELALM
                     MSISLRFIPTLMDETDKILKAQLARGSDLTSGTVKERIRAVVPLLVPLFVSAFKRAED
                     LAIAMEVRGYRGGEGRTRYRQLNWQWRDTLVIIVFTLLVAALVWLRGGN"
     gene            complement(3667..>4513)
                     /locus_tag="VFX34_04160"
     CDS             complement(3667..>4513)
                     /locus_tag="VFX34_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5564132.1"
                     /translation="GYSYAKGTPFEKRALYEVNASIESGSYTAVIGHTGSGKSTLLLH
                     LNGLLKPTEGIVRVGDINITSDTKAKELKTIRKHVGIVFQFPEHQLFEETVEKDIMFG
                     PLNFGIPEEEARARAHELIKLLGLPEDVAQKSPFDLSGGQMRRVAIAGVLAFKPSILI
                     LDEPTAGLDPRGRKEIMELFNKLHKEEGLTTILVTHSMEDAAKYADQILVMHEGRSVM
                     AGTPEEVFGNAEQLQSFRLGLPRSVAFQRDVEKLFGKRLGKLALTEEQLAGAIADALK
                     EEGER"
BASE COUNT         1130 a         1150 c          984 g         1249 t
ORIGIN      
        1 gttcacgcac atccaacgtc aaaggcactt gatttgtgct ttcgagcgcg accaaatcaa
       61 tttcgatgct tgtctcccat acccggtgcg cagcttccgg atcccagaat acattccatt
      121 ccgcagttcc atcatgctcg ggttcatgaa cgtttccatc ttctcggaaa gttccgccca
      181 tccaaacgag tctttcgatt ttctcttcaa tatccggcgc ttcgtccaat gcgcgggcaa
      241 gatcagtaag cggacctgta aacaataaag tcgtcttttc tgaagtgttc cggattgcat
      301 cgatcatatg aagatgtgcc ggtttatccg caactggcgt atcaattttt ccggattcat
      361 ttaaaattgg cagtgcgtcg acaagaaacg catgcattct ccagtcttta ggaaaaggat
      421 tctttccacg cgagttcgat tcagctacat cgaggttgac gccatttcca aagcgatcta
      481 tgattttccg gcttgcataa accgccggct ccaagtaaca gtcggctgga atgaccgata
      541 cgcctgtcag ttcaatatta tccattcgaa gcaataagaa taatgatatg agatcatcta
      601 caccgccatc gtggttgaaa taaataggtt ttttcgtcat gctgatccgt ccttcacttt
      661 gaattgatat ggatagtctc ccgttattag agagaagata ttcatatgtt tcttccgtca
      721 tttcatcttc caatgaaacg ccaaaaaacc gggtataaat tctacccggt tcaccgattc
      781 acgaaaaata cggaaattgt atatttccat cattcatgta gtccggtagt ttacggttac
      841 caggtagaaa cgcttaagcc atattcccaa gcatatacat tcactattca catgttagag
      901 ctgtgcggtt tgcacagctg tttttttact ttacatttat agcatagaaa ctttagcatg
      961 gtcaataaaa acacgaatgt tgttttattt tgataactcg tctttcgtgt ttacgctttc
     1021 agggtaatga aaattctata aacaataaaa aaccccttcc cgataaatag gggaaagggt
     1081 tgaaattgtt cgtagattaa cgttttgaga actgaggtgc acgacgcgcg cctttaagac
     1141 cgtatttttt acgttctttc atgcgggagt cacgagtaag caagcctgca gatttcaatg
     1201 tgccgcggaa gtcaggatct acagtcaata gagcgcgtgc aacgccgtgg cggattgcgc
     1261 ctgcttgacc tgtgtatccg ccgccgtgga cgtttacatg gatgtcatag ctgccaagtg
     1321 tttgagtagc tacaagtggt tgcttgatca cttcacgaag tgtttcgaat ggtacgtagt
     1381 cttctacgtc acggttgttg acgacgattt taccttcgcc aggtacgaga cgtacacgag
     1441 ctactgagct tttacgacgg ccagtgccga tatattgtac ttgtgccaaa agtgtttcct
     1501 cctcttaatt atccgcgaag ctcgtatgct tctggttttt gtgccgtatg tggatgttcc
     1561 ggtccagcat agacatgaag tttcttgatt gtttgacgac ctagagggcc ttttggaagc
     1621 atccctttaa tcgcaagttc caacatttta gttggatagt tcgtacgcat ttcaagcgca
     1681 gtacgttgtt tcatattacc tgtataacca gtgtggcggt agtagatttt atcttttaat
     1741 ttattcccag acagttggat cttctcagcg ttgatgatga tgacgtgatc gccagtgtca
     1801 acgtgtggtg tgaacgtagg tttgtttttg ccgcgcaaaa ttgctgcaac ttcagaagct
     1861 agacgaccaa gcgtctgtcc ttcagcgtcg acaacgagcc atttacgctc tacttcgtga
     1921 cctttagcca tgaatgttgt acgcatgtat tgtttcctcc taaaaatatc aatctgttcc
     1981 gttgttttct ctatccctaa acacaaataa actttccggg gcttattgtg gggttattga
     2041 aataccattt atcatcttat attgaatgca aacataagtc aagcatttta ttagaaaacg
     2101 cctggaaaag ttgacacgag attagtacgt gacctccatc aagtacaaac cgtgtgccgg
     2161 cgcggttttc ccggcctttt tccggtcctt cgcctcaagg atttccttca tatcctgcgg
     2221 ctcgttccag ccgatcccta cggcaaaaag catgcctgcg atcgtccgga ccatattgta
     2281 caaaaaacca tcgccttcaa ttgtcatgat cagctcttcg ccgcgtcgtt ctaccgtcaa
     2341 ttcccggacc gtccgcactt tattctccgt cgcggtcttg gatgaacaga aactcgtaaa
     2401 gtcatgcgtg ccgataatgt acttggctgc ctcgcacatc ctttcgatat ccggccgcca
     2461 cttccccaaa tggacggaat aattccgttc aaacgggctt tgcacttcgc tcgaagtcca
     2521 tttgaacata tacgttttcc ctgtcgcgga atatcgtgcg tggaagtcat catctacgta
     2581 ttccgttttg acaatccgaa catctttcgg caataaaaca ttcagcgcca tttgccagcg
     2641 atcaagcggc agctccagag gcgtatcaaa atgaatcacc tggccccatg catgcacgcc
     2701 agcgtccgtc ctgccactag caaccgagaa aatcgaactg tctttatgga gcttcacaag
     2761 cgccttgtcg atgacggatt gcaccgtccg catattcggc tgaaactggt atcctgcata
     2821 gcctgtaccg tcgtaagcga cagtagcctt gacccgtctc atatcagttc cctcccctca
     2881 accaaacgag agcggcaact agaagagtga aaacgatgat aaccaacgta tcacgccatt
     2941 gccaattcaa ttgacggtac ctcgttctgc cttctccgcc gcggtaccct ctcacttcca
     3001 ttgcgatggc gagatcctcc gcccgtttaa atgcactgac gaaaagaggg acaagaagcg
     3061 gcacgactgc ccgaatcctt tctttgaccg ttccgctcgt caaatccgaa ccgcgggcga
     3121 gctgcgcttt caatatttta tcggtttcat ccattaaagt cgggatgaaa cgaagtgaaa
     3181 tcgacatcat caaggcaagc tcgtgaacag gcaatttaat tcgcttcaag ggattcaaca
     3241 gcgtctccat tccgtccgtg atggaaatgg gagatgtcgt caacgtcaat atggtcgtca
     3301 tcagaacgag gacgaggaac cggatggaaa tatagatccc ttgcctgacc ccttcttcgt
     3361 aaatcttaat gaatttccag tcgacgagca gtgcgccttc cttcgtgaag atgaagtgca
     3421 tgagcaatgt gaaaatgatg aggaatagga tcggcttcaa tccgttgaac aggaaataga
     3481 gccggatacg cgcaatagag atgacgaaca atgtaaatgc caataacaaa gtgtatgtca
     3541 cggtattatt cgctaaaaat accgcgacaa tgaacatgaa gacgaacagc agcttcgatc
     3601 tcggatccag ccgatggacg aacgaatcac ccggtacata tcggccgaag atcatctttt
     3661 ccagcattac cgctcacctt cttccttcag agcatctgca atcgctcccg caagctgctc
     3721 ttccgtcagt gccagctttc ctaaccgctt gccgaacagc ttctccacat cccgttgaaa
     3781 cgcaacagaa cgcggtagac cgagccggaa cgattgcagt tgctcagcat tcccgaacac
     3841 ttcctcgggc gttccggcca tgacagaacg cccttcatgc atgaccagga tctgatcggc
     3901 atatttcgct gcatcctcca tgctgtgcgt gacgagaatg gtcgtcaagc cttcttcttt
     3961 atgcaattta ttgaaaagct ccatgatttc cttccgtccg cgcggatcga ggccggctgt
     4021 cggttcatcc aagatgagga ttgacggttt gaatgcaagg acacccgcaa tcgcaaccct
     4081 tcgcatctgt cctccggata ggtcgaacgg cgatttctgc gcgacatctt ccggcaatcc
     4141 caacagtttg atcaattcat gtgcacgagc tctcgcttcc tcttctggaa tcccgaagtt
     4201 cagcggaccg aacatgatat ccttctcaac ggtttcttcg aagagctgat gctccggaaa
     4261 ttggaacacg atgccgacat gcttgcggat cgtcttcagc tctttcgcct tcgtgtcgga
     4321 agtgatgttt atatcaccga cgcgcacgat cccttccgtc ggcttcagaa ggccattcaa
     4381 atgtaggagc agcgtcgatt ttccggaccc tgtatgcccg atgaccgcag tatacgatcc
     4441 cgattcaatt gaagcattca cctcataaag ggcacgtttt tcaaaaggcg ttccttttgc
     4501 gtatgaatag cct
//
LOCUS       DASULI010000193         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1889884,
            whole genome shotgun sequence.
ACCESSION   DASULI010000193 DASULI010000000
VERSION     DASULI010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889884"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1859
                     /gene="lon"
                     /locus_tag="VFX34_04165"
     CDS             <1..1859
                     /gene="lon"
                     /locus_tag="VFX34_04165"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5564133.1"
                     /translation="IEEPGRLADMIASHLPLKISAKQEILEITDVKKRLEFLINKLHD
                     EQAILALERKINTRVKEAMERTQKEFYLREQMKAIQKELGDKDGKSLEVVELTKKIEE
                     AGMPENVKQAAFRELDRYEKAHSASAESGVIRNYIDWLVALPWSDASKDQLDINRSEE
                     ILNRDHEGLESVKERILEYLAVRQMTNSLRGPILCLDGPPGVGKTSLARSIAESLGRK
                     FVRISLGGVRDESEIRGHRRTYVGAMPGRIIQGMKKAGKINPVFLLDEIDKMSNDFRG
                     DPSSAMLEVLDPEQNSTFSDHYIEEPYDLSNVLFVATSNDLSSIPGPLRDRMEIISIP
                     GYTELEKRAIAKNHLIPKQLKEHGLTKSQVRFNDDAIRNIVRYYTREAGVRGLEREIA
                     RICRRAAKQIVTGEKKSVTVSVNTIETLLGKRKFSYGQAEKKNQIGVATGLAYTTVGG
                     DTLQIEVSLSPGKGKLILTGKLGDVMKESAQTALSYVRSRAVDFHINPNFHESSDIHI
                     HVPEGAVPKDGPSAGVTIATALVSALTKRPIRREVGMTGEITLRGRVLPIGGVKEKSL
                     SAHRAGLTTIILPIANERDIEDIPESVRNELTFKLVSDAEEVLEYALEEASE"
     gene            1856..2437
                     /gene="yihA"
                     /locus_tag="VFX34_04170"
     CDS             1856..2437
                     /gene="yihA"
                     /locus_tag="VFX34_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX5564134.1"
                     /translation="MKVNQVEMIMSAVKPEQYPDDGFPEFALAGRSNVGKSSFINKMI
                     GRKSLARTSSKPGKTQTLNFYKIEEKLFFVDVPGYGYAKVSKSEREAWGKMIERYLTG
                     REQLRAVVLIVDLRHPPTVDDCLMYDFLGHYHIPTIVIATKADKIPRGKWEKHKKIMK
                     NELNMRAGDPIILFSSEKGIGMDEAWREIENRM"
BASE COUNT          734 a          505 c          671 g          583 t
ORIGIN      
        1 ctatcgaaga accgggcaga cttgcagata tgatcgcttc ccatcttccc ttgaagattt
       61 ctgccaaaca agagatcctg gaaatcaccg acgtcaagaa gagattggaa ttcctcatca
      121 ataaacttca tgatgagcag gcgatcctcg cattggaacg gaaaatcaat actcgtgtaa
      181 aagaagcgat ggaacggacg cagaaagaat tttatttgcg cgagcagatg aaggcgatcc
      241 aaaaagagtt gggagataaa gacggcaaga gccttgaagt cgtcgaattg acaaagaaaa
      301 ttgaagaggc tggcatgcct gagaacgtga aacaagctgc attccgtgaa ctggatcggt
      361 atgagaaggc ccattctgca tctgctgaaa gcggcgtcat ccggaactat atcgattggc
      421 tcgtcgcatt accctggtca gatgcatcga aggatcaatt ggacattaat cgttccgagg
      481 aaatactgaa ccgcgaccat gaggggcttg aatcggtgaa ggagcgtatt ctggagtacc
      541 ttgccgtccg tcaaatgaca aactccttgc gggggccgat cctttgtctt gatggaccgc
      601 ccggcgtcgg aaagacttct ttggcacgtt cgatagcgga atcattgggc cgcaaatttg
      661 tacggatctc cctcggcggc gtacgggacg aatctgaaat cagaggtcat cgtcggacat
      721 atgtcggggc gatgccagga cggatcattc aaggaatgaa gaaagcagga aagatcaatc
      781 cggttttcct tctcgacgaa atcgataaga tgtcgaatga tttcaggggt gatccatcat
      841 cagctatgct ggaagtcctt gatcccgagc agaactcgac attcagcgat cactatatag
      901 aagagcctta tgatttgtcg aatgttttat ttgtcgcaac ctccaatgat ctaagttcga
      961 tccccggacc attaagggac cggatggaga ttatctccat accgggatat acggaattgg
     1021 aaaagcgggc cattgcgaaa aatcacctca ttccgaagca gttgaaggag catgggttga
     1081 cgaaatctca agtccgattc aatgacgatg caatccggaa tatcgtacgt tattatacgc
     1141 gcgaagccgg tgtccgcgga ttggagaggg aaattgcgag gatttgcagg agggccgcaa
     1201 agcagattgt gaccggggaa aagaaaagtg tcacagtcag tgtgaataca attgaaacgc
     1261 ttttaggcaa aaggaagttc agctatggac aggccgagaa gaaaaatcag atcggcgtcg
     1321 caactgggct tgcctataca actgttggag gagatacact ccaaattgaa gtttcattgt
     1381 ccccgggcaa aggtaaactg atattgacag gcaagctggg tgatgtcatg aaggaatcgg
     1441 cgcaaactgc attgtcttat gtaagatcaa gggcggtgga ttttcatatc aatccgaact
     1501 tccatgaatc aagtgacatc catatacacg ttccagaagg tgcggttccg aaagatggtc
     1561 cttccgcggg tgttacgatt gcgacagcgc ttgtttcagc cttgacgaag cggccgatcc
     1621 ggcgtgaagt cggaatgacg ggagaaataa cgcttcgagg cagagtgctg cctattggag
     1681 gcgtgaagga aaagtctctg agcgcgcatc gggcaggctt gacgacaatc atcttgccga
     1741 ttgccaatga acgagatatt gaagatatac ctgaaagtgt acgtaatgag ctgacgttca
     1801 agcttgtctc agatgctgag gaagttttgg agtatgcatt ggaggaggca tctgaatgaa
     1861 agtcaatcaa gttgaaatga tcatgagtgc ggttaaaccg gaacaatacc cggatgacgg
     1921 tttcccggaa tttgcattgg cgggacggtc gaatgtgggg aaatcttcgt tcatcaataa
     1981 aatgatcgga cgtaaaagtt tggcccgtac atcctcgaag cccggtaaga cccaaacgtt
     2041 gaatttctat aaaatcgaag aaaaactatt tttcgttgac gttcccggtt atggctatgc
     2101 gaaagtgtcc aaatcggaac gtgaggcatg ggggaaaatg atcgagcggt atttgaccgg
     2161 tcgagagcaa ttacgggcag ttgtcctgat tgtcgacctt agacatcctc ctactgtaga
     2221 cgattgcctg atgtatgatt ttttaggtca ttatcatatt ccaacaattg tcattgcaac
     2281 gaaagcggat aaaatcccga gaggcaagtg ggaaaagcac aaaaaaatca tgaaaaacga
     2341 attgaatatg cgagccggag atccgatcat ccttttctct tccgaaaaag ggattgggat
     2401 ggatgaggca tggcgcgaga ttgaaaatag aatgtaatca cgatccggat gaaaagttca
     2461 tccggatttt cggttaagtg gagtagaaac aca
//
LOCUS       DASULI010000194         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_387314, whole
            genome shotgun sequence.
ACCESSION   DASULI010000194 DASULI010000000
VERSION     DASULI010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387314"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFX34_04175"
     CDS             <1..357
                     /locus_tag="VFX34_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX5564135.1"
                     /translation="VESLAYQSKDVLLTMEKDAGIDIDILRVDGGAVANTFLMQFQSD
                     LLQIEVEQSAYLETTALGAAYLAGLATGFFESCESVEALWAKERTYSPTMDVEERDAL
                     YSGWKKAVSAARAFKL"
     gene            375..2021
                     /locus_tag="VFX34_04180"
                     /pseudo
     CDS             375..2021
                     /locus_tag="VFX34_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
     gene            2018..2932
                     /locus_tag="VFX34_04185"
     CDS             2018..2932
                     /locus_tag="VFX34_04185"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5564136.1"
                     /translation="MNEQMITMSDGFSIYTINAQPEDRPIAHIHLLHGMAEHIGRYED
                     FITYLTNEGFAVSGQDHRGHGQTAAFNGKLGHFGDAAGFNRIVDDAHEIISYYKTKFK
                     APKVILIGHSMGSFIARRYVQRFGKEIDKLICIGTGGDPGASRIGGQALAYMKGQATK
                     FDEPDYFINKLVFGGFNKVVDQPKTSFDWLTSDDSVVEKYINDPLCGFVPTTRFFIDL
                     FNGLAKINDQEKIRQVPIGLPILLLSGLDDPVGNKGKGIWQAARQFTDAGIENVTVML
                     YEGGRHEMLNEVNREQVFNFIKDWIIEE"
     gene            2929..>3030
                     /locus_tag="VFX34_04190"
     CDS             2929..>3030
                     /locus_tag="VFX34_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEX5564137.1"
                     /translation="MKQSERVIAIVGPTASGKTSLSIQLAKRLDGEII"
BASE COUNT          854 a          636 c          798 g          742 t
ORIGIN      
        1 gtcgaatcgc tggcatatca atcgaaggat gtactactga caatggaaaa ggacgccggt
       61 atcgatatcg atatcttgcg tgttgacgga ggcgctgtgg ctaatacatt cctcatgcaa
      121 ttccaaagtg acttgcttca gatcgaggtg gagcagtcgg cctatttgga gacgacggca
      181 cttggagcgg catatcttgc agggcttgca accggcttct ttgaatcatg cgagtcagtt
      241 gaagcgttat gggcgaagga gcggacgtat tcacctacta tggatgtgga agagcgtgac
      301 gccctataca gcggctggaa aaaggctgtc tcggcagcaa gagctttcaa actttgactg
      361 gaggagagcg aaggatgtta tcatcgatca caagaccgaa agtgaaacaa ttcatgcgcg
      421 actatcagtt tgacctgctt gtcattggcg gggggattac cggcgctggc attgcgctgg
      481 atgcggtgac aagaggatta tccgttgcct taattgacat gcaggatttt gcggcgggca
      541 cttccagcag gtcaacgaag ctcatccatg gagggttgag gtatttgaag cagatggaat
      601 tgaaaatcgt ttcggaaact ggcagggaaa gggatatcgt ctatcggaat gcccttcatg
      661 tcacagagcc ctagcggatg cttcttccat tccataaagg cggctctttt ggaaagttga
      721 caacttctgc cggattattc gtctatgata aattggctgg tgtcaaaaaa gaggagcgcc
      781 gtgaaatgct gtctgccgaa gagacgctcg ctatggagcc attggtccgg gaagaaggtt
      841 tgcttggcgg ggggcattat gttgaataca ggacagatga tgcacggctg acgattgaag
      901 cattgaaaaa agccgcgaca aaaggcgcgc tttgcttgaa ctacatgaaa gcagagcagt
      961 ttcaatataa agatgggaaa gtgtcaggtg tcactgcacg agacatgctt tctggtgaag
     1021 tgatgaatat aagcgcctct gtcgttgtca atgcgactgg cccctgggtg gatgaaatgc
     1081 gggaaaagga cgaagtgtca aatgacaaac gtctacgttt gacgaaagga acacatattg
     1141 ttattgacaa ggcaattttc cctttgcgtc aagcgatcta ttttgattca cctgacggaa
     1201 ggatgatctt tgccattccg aggggcaata aagcctatat cgggacgacg gatacatttt
     1261 ttgacggcga tcgtgcctat cccgttgcga gcgaagagga tatttcctat ctccttgatg
     1321 cactccatta tatgttccct tccgtcaaag cgcgtcgcaa tgatatcgaa tcgacttggg
     1381 cgggcgtgcg gccgcttatt tacgaagaag gaaaagacgc atccgaaatt tcgcgtaaag
     1441 atgaaatttg ggaagcaggc acgggtttga tcacaattgc gggcggcaaa ttgacgggct
     1501 accgtaaaat ggcggaaacc gtcgttgatc atgtcgtgaa gcgtttccct actacatcgt
     1561 tcggtccatg catcacggaa catctcccac tttccggcgg agacttcgag agtgaggaac
     1621 agtttgcgaa cttccttgaa agcaaatcga atgaatctga attatacggt cttagcaagg
     1681 aagaaggccg ttcgctcgct tccttatatg ggacgaatgt cgattacgtc ttcaactatg
     1741 tcagtgccgt gtcaacggag agcgctgtgc tgccggcaat atggaaagct gaaatcctat
     1801 atgcgatcca ttatgaaatg gccatgacgc catcggactt tttcattcgc aggaaaggtg
     1861 acctctactt caacatctcg tttgccaaac tgtacaaaga ggcggtcact gattatatgg
     1921 gcgccatcct ttcctacaat gacgaaatga gagagcatca cctatcacaa ttagaacaac
     1981 atataaatga agcagaagga aaggccggtg aacgtatgtg aatgaacaga tgataacgat
     2041 gtccgacggt ttttccatct acacgattaa cgcacaacca gaagatagac ccatcgcgca
     2101 tatccatctt ctccacggta tggcggagca tatcggccgt tacgaagatt tcataacata
     2161 tctcacaaac gaaggatttg ccgtatccgg gcaagatcat cgcggccatg ggcagacagc
     2221 ggcgtttaac gggaaattag gtcatttcgg cgatgctgca ggatttaatc ggattgtcga
     2281 tgacgcgcat gaaatcatca gctattacaa aactaaattc aaagctccaa aagtgattct
     2341 catcggtcac agcatgggtt catttattgc aagaagatac gttcagagat tcggaaagga
     2401 aattgacaag cttatttgca taggaaccgg cggcgatcct ggcgcaagcc gaatcggcgg
     2461 gcaggctcta gcatatatga aaggacaggc aacgaaattc gatgagccgg attatttcat
     2521 taataaattg gttttcggcg ggttcaataa agtggtggat caaccgaaaa cttctttcga
     2581 ctggctaaca tcagatgata gtgtagttga gaaatatatt aatgatccgt tatgcggatt
     2641 cgtcccgaca acccgttttt tcattgactt gttcaatggc ctggcgaaaa tcaatgatca
     2701 ggaaaagatt agacaagtcc ctatcggact gccgattctc cttctttccg gattggacga
     2761 tcctgtcggc aataaaggaa aaggcatctg gcaggctgcg cggcaattta ccgatgccgg
     2821 catcgagaat gtaaccgtca tgttatatga aggcggccgc cacgaaatgt tgaatgaagt
     2881 gaatcgggaa caagtattta atttcataaa ggattggata atagaagaat gaaacaatcg
     2941 gaaagagtga tcgcaattgt cggaccgaca gcatccggca aaacatcatt gagcatccag
     3001 ctagctaaaa ggctcgatgg ggaaatcatt
//
LOCUS       DASULI010000195         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_653406, whole
            genome shotgun sequence.
ACCESSION   DASULI010000195 DASULI010000000
VERSION     DASULI010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653406"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFX34_04195"
     CDS             complement(<1..692)
                     /locus_tag="VFX34_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5564138.1"
                     /translation="MKKVLSALLLTVLVLALAACGAKDKDGASKNAAGGKEESTKLVV
                     GASNTPHAVILEKVKPLLKEKGIDLEIETYQDYVLPNKDLESGLLDANYFQTIPYLES
                     QIADFGYDFVNAGGIHIEPMGVYSKKYKSLDELPDGATVLMSNSVSDHGRVLEMLEKE
                     GLITLDEGVDKVKAELSDIVDNPKNLKFEPDYEAALMPTLYKNDEGDALLINSNYAID
                     AGLNPLEDSIAI"
     gene            complement(747..1415)
                     /locus_tag="VFX34_04200"
     CDS             complement(747..1415)
                     /locus_tag="VFX34_04200"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEX5564139.1"
                     /translation="MNEELFQHVNWDKMWEATVETLYMTGLSTFYTFIFGLLLGVLLF
                     LSSPGQLWQNKLIYGITGAFVNIFRSIPFIILIILIIPFTTLLVGSMRGPEAAIPALV
                     IGAAPFYARMVMIGLREIDRGVVEAARSMGAKTSTIIFKVLLPESMPALISGITVTAI
                     ALVGYTAMAGVIGAGGLGNLAYYEGFQRSRTDVMIVATIIILIIVFAIQIIGDFAVKK
                     MDKR"
     gene            complement(1408..2421)
                     /locus_tag="VFX34_04205"
     CDS             complement(1408..2421)
                     /locus_tag="VFX34_04205"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEX5564140.1"
                     /translation="MIQLKDVKKSFGRTNDEILAVDGVSLTIDEGEIFGIIGYSGAGK
                     STLIRLLNGLETPTTGTIKIGDREISAFTGRELREARQKISMIFQHFNLLWSRTVKQN
                     IAFPLEIAGVKKAERERKVAELIELVGLSGREDAYPSELSGGQKQRVGIARALANDPE
                     VLLCDEATSALDPETTDAILDLLTSINERLGLTIVLITHEMHVIRKICHRVAVMEAGK
                     VVEIGPVLDVFQSPQAPITKRFVSQVTEPAGTDQAIAHLPGGTLIKLVFVGEKTEHPV
                     LASLIRKFDIDVNIVQGNISHTKGGAYGSLILQLLGNENDIDTAIAYLHELGVQTEVI
                     GNE"
     regulatory      complement(2517..2621)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(2678..2977)
                     /locus_tag="VFX34_04210"
     CDS             complement(2678..2977)
                     /locus_tag="VFX34_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5564141.1"
                     /translation="MENWTREQWEMSKNEFPESIYYLYTPMCGTCAVASKMMEVIAAM
                     KPGLPIGKADLNYVEEMAVDYEIESVPCLLIQKDGVVVHKIYAFQSIPHLLEKLG"
     gene            complement(2999..3340)
                     /locus_tag="VFX34_04215"
     CDS             complement(2999..3340)
                     /locus_tag="VFX34_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX5564142.1"
                     /translation="MEYRKVLIVEGGSDRKRLQKVLAEPVEIICTNGTVSPYRIEEIL
                     TPYEDCELFVFLDADESGDKIRSLFKRYYPEATHLYTERTYRQVETTPYRVLAAVLLS
                     ENFEVHPEFLL"
     gene            complement(3582..3965)
                     /gene="gcvH"
                     /locus_tag="VFX34_04220"
     CDS             complement(3582..3965)
                     /gene="gcvH"
                     /locus_tag="VFX34_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003327010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5564143.1"
                     /translation="MSTPKDFRYSEEHEWVKDEGGKYRIGITHFAQSELGDIVFVELP
                     QVGDEVKADEPFGSVESVKTVSELYAPISGKVVEVNEDLEDSPELVNESPFEGAWMIV
                     VEASDDSELDKLMSAEDYDKMTVGE"
     gene            complement(4046..>4289)
                     /locus_tag="VFX34_04225"
     CDS             complement(4046..>4289)
                     /locus_tag="VFX34_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012960355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEX5564144.1"
                     /translation="KEELQQMIENSGLEIKKFFNVSGMKYRELNLKDKLPGMTEAEKV
                     ELLATDGMLMKRPIVTNGSKVTVGFKEEQFEEVWGA"
BASE COUNT         1095 a         1120 c          863 g         1211 t
ORIGIN      
        1 tcaattgcaa tcgaatcttc aagtgggttc aatccagcat cgatcgcata gttggaattg
       61 atgagaagcg catcgccttc atcattttta taaagtgtcg gcattagagc tgcttcataa
      121 tctggttcaa acttcagatt cttcgggttg tccacaatat ctgagagttc cgcttttact
      181 ttatcaacac cttcatcaag tgtgatcaat ccttcttttt caagcatttc aagcacacgt
      241 ccatgatccg aaaccgagtt gctcataagg accgtcgcac cgtccggcag ttcatccaat
      301 gatttatatt tcttcgaata aacacccatc ggttcgatat ggattcctcc cgcgttgaca
      361 aagtcgtagc cgaaatcagc aatttgactc tccaagtaag ggatggtctg gaaatagttc
      421 gcatctagga gacctgattc caagtccttg ttcggcaaga catagtcctg gtatgtttcg
      481 atttccaagt cgatgccttt ttccttcaac aacggcttca ctttttctaa aatgactgca
      541 tgcggtgtat tggaagcgcc gactacgagt ttcgtcgact cttctttgcc gcccgcggca
      601 tttttgcttg ctccatcttt atccttcgct ccacatgccg ctaatgcaag cacgagaaca
      661 gtcagtagta atgccgataa tacttttttc atgttttcct ctccccattc ttagatgata
      721 tttataatta acaggaattc ctgcaattaa cgtttgtcca tttttttcac agcaaagtcg
      781 ccgatgattt ggatcgcaaa gacgataatc aaaatgatga tcgttgcgac aatcatcacg
      841 tccgtccggc ttctctggaa tccttcataa taggcaaggt tcccgagccc gccagcaccg
      901 atgacacctg ccatcgcagt atagccgaca agcgcaattg cagttaccgt aatccccgaa
      961 atgagtgcgg gcatcgattc aggcaataaa accttgaaga tgattgtgct tgttttcgca
     1021 cccatcgatc tcgcggcttc gacaacgcct cgatcgattt cacgcaagcc gatcatgacc
     1081 atccgcgcat aaaacggtgc agcaccgatg acgagagccg ggatggccgc ttccggtccg
     1141 cgcatcgacc ctacgagcaa ggtcgtgaaa ggaataatta gtatgatcaa aataataaaa
     1201 gggatcgacc ggaaaatatt gacgaatgca ccggtgatgc cgtagattaa tttattttgc
     1261 caaagctgtc ccgggcttga taagaataga agtacgccaa gtaagagccc gaatataaat
     1321 gtataaaaag tcgagagccc cgtcatatat agggtttcaa ctgtagcttc ccacatttta
     1381 tcccagttaa catgttgaaa taattcttca ttcattgccg atcacctctg tctgtacgcc
     1441 tagttcatgg agataggcaa tcgctgtatc aatatcattt tcattgccga gaagctgtaa
     1501 aatgagcgag ccataggcac cgccttttgt atgggagata ttcccttgga cgatattcac
     1561 gtcaatatcg aatttccgga tcaagcttgc caacacagga tgctccgtct tctctccgac
     1621 aaagacgagt ttgatgagcg tccctcccgg tagatgggct atcgcttgat cggttccagc
     1681 cggttccgtc acttgggaaa caaatcgctt tgtaatcgga gcttgaggtg actggaacac
     1741 gtccagcaca ggaccgattt caacgacttt acctgcttcc atgaccgcca cgcgatggca
     1801 gattttccgg atgacatgca tttcatgtgt aatcagtacg attgtcaaac cgagccgttc
     1861 attgatactt gtcaacagat ccaggatggc atcagtcgtt tccgggtcaa gcgcggaagt
     1921 cgcttcatcg caaagcagca cttcaggatc atttgccaac gcccgggcaa ttcccacacg
     1981 ttgcttctga ccgccggaaa gttcggatgg ataagcatct tccctgccgc tcaatccgac
     2041 aagctcgatc agttccgcca ctttccgttc gcgttccgct ttcttcactc cggcgatttc
     2101 aagcgggaaa gctatattct gcttcactgt acgggaccaa agcaaattga aatgttggaa
     2161 tatcatgctg atcttttgtc ttgcttcccg cagctctctt ccggtaaacg ccgatatttc
     2221 acgatctcct attttgattg ttccggttgt cggagtctca agaccattca ataggcggat
     2281 caatgtactt tttcctgcac cgctataacc gatgatgccg aaaatttcac cttcatcgat
     2341 cgtcagggag acaccgtcca ccgccaggat ttcatcattc gtccggccga aacttttttt
     2401 cacgtctttc agttggatca tcattatcac ctctaccact atcttgtcca aaataaaaac
     2461 ctttctgcag acaagcagaa aggctaaaac gttctattaa taaacgtcaa tccgatctct
     2521 catctctcaa agcaaacgct ttgtgtgact tggcaccatt tcacttccgt gacggttgcc
     2581 gggcttcata gggcacttcc ctccacctct ctctataaga gcgacatatt cagttaaagt
     2641 taatagatat acttatatca tggatatctg acatatgtca acctaatttc tcgagaagat
     2701 gcggaatgga ttggaatgca tagattttat ggacaactac cccgtccttc tgaatcaaca
     2761 gacaagggac gctttcgatt tcataatcga cagccatttc ctccacataa ttcaagtccg
     2821 ccttcccgat tggaagaccc ggtttcatgg cagcgatgac ttccatcatt ttcgaagcaa
     2881 ctgcacaagt tccgcacatc ggggtatata aataataaat cgattccgga aattcgtttt
     2941 tgctcatttc ccattgttca cgtgtccagt tttcgatgtt ggccactatt gatcaccttc
     3001 aaagtaaaaa ttcaggatgc acttcaaaat tctcagagag gaggacggcg gcaagcacgc
     3061 ggtacggcgt cgtttctact tggcgatacg tccgttccgt ataaagatgg gtcgcttccg
     3121 gatagtacct tttgaatagg gatctgattt tatctccgga ttcatccgca tccagaaaaa
     3181 cgaagagttc gcaatcctca taaggcgtga ggatctcctc gattcgatag gggctcacag
     3241 tcccgttcgt gcaaatgatt tcaaccggtt ccgccagcac tttctgcaat cttttccggt
     3301 ccgaaccgcc ttcgacgatc aagactttcc gatattccac ttgcacgaca cccccttaac
     3361 caaatgcgta gggcggcgat tagcctcgac aggcgctgga gggcttggaa aaaaaggcgg
     3421 tctttgcctt ttcttttcaa gcctgtagcg acccgagagg ctgccacccg aagctagatg
     3481 agactaccac attagaagtt tacccaccct atctggaatt tatcgttact cttccattga
     3541 aaaaagcgcc ggagtcaaag atccaggcgc cactttaacg attactcgcc gacagtcatt
     3601 ttgtcgtagt cttcagctga catgagttta tcgagttcgg agtcgtcgga tgcctctaca
     3661 acgatcatcc aagcaccttc aaatggagat tcgttcacca gttcagggct atcttccaag
     3721 tcctcattca cttcgacaac tttaccgctg atcggagcat acagttcaga aacagtttta
     3781 actgattcaa cgctgccgaa tggctcgtcc gctttcactt cgtccccgac ttgcggcaat
     3841 tcaacgaaaa cgatatcgcc aagttcggat tgtgcgaaat gtgtaatacc gatacgatac
     3901 tttccgcctt cgtcctttac ccactcatgt tcttctgaat aacgaaaatc tttaggtgtg
     3961 ctcatcccgt acccctccat aagtatacaa ttattgattt ccattcaatt ttgacatatt
     4021 ccgatgctaa agacaagaaa gtatattagg ctccccaaac ttcttcaaac tgctcttcct
     4081 taaagcccac agtcactttc gagccatttg tcacaatcgg ccttttcata agcatgccat
     4141 ccgtcgccag cagttcgacc ttctcagcct ccgtcatccc aggcagctta tctttcaaat
     4201 tcaactcacg gtatttcatt ccgctcacat tgaagaactt cttaatctcc agtccggagt
     4261 tctcgatcat ttgttgaagc tcctctttt
//
LOCUS       DASULI010000196         5150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_998127, whole
            genome shotgun sequence.
ACCESSION   DASULI010000196 DASULI010000000
VERSION     DASULI010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998127"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFX34_04230"
     CDS             complement(<1..273)
                     /locus_tag="VFX34_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564145.1"
                     /translation="MPVSFRKHEEFFTFHDPMQDGKLIDRKTEIRFDPLTGETSRVIF
                     DPGAPFMPKDYSELAKATEGEKCPFCAENVSKITPRFPDELVEGGRF"
     gene            complement(345..1364)
                     /locus_tag="VFX34_04235"
     CDS             complement(345..1364)
                     /locus_tag="VFX34_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007130757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEX5564146.1"
                     /translation="MIRYPKPLQQGDTIGVTAPSSGVEETLHHLIHNAKRQFEKRGYQ
                     VNVGDTVWTQIQAASATKEVRAAELMAMFNNPEIHAIIPPWGGEILIEILPLIDWDEI
                     YPKWILGYSDMSTFLFALTVKTGIATAHGPNFVEMRSDEWEPVASKFMDVLQAPAGTS
                     IEQHSSEKFQSKWQHDAPDDPYVYKLDSPTEWKVIGSTNIELEGRLLGGCMDTIRHLV
                     GTPYGDIASFQKNFLDDEPILWYFENCEMNATDFHRTMLQFVYAGWFKNAAGIIFGRS
                     PAGKEVEGFTVIDSMERIKEMTGLPIIYDADVGHVPPQITFVNGAYGKVTVNNGKGFV
                     KTTLR"
     gene            1466..1951
                     /locus_tag="VFX34_04240"
     CDS             1466..1951
                     /locus_tag="VFX34_04240"
                     /inference="COORDINATES: protein motif:HMM:NF044334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564147.1"
                     /translation="MMKQGGFIVTLLAMMFLSGCGHSETNAIYLLPEGYTGSAIAVYN
                     VEGAPVLTYEGDFAVYRINEDGYFATSLPDMQYGTVTDRYYYVDPAGNRTPIDPNCIH
                     LFGTGAVSTDKVDLVYTGIEVTDSCSEEFANSTENFSEEPKMEILNKVAKKFYGVGLQ
                     P"
     gene            1967..2263
                     /locus_tag="VFX34_04245"
     CDS             1967..2263
                     /locus_tag="VFX34_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564148.1"
                     /translation="MALLKYKRMFLLSVLLAAFSIFFLPQTEYGFGWPIPWLEVHGNK
                     TMITGSELLQVMNVKNTFFDLWNLLLGSLIIYGMLFIAYKGLNKLNKDTEIGEK"
     gene            2266..2913
                     /locus_tag="VFX34_04250"
     CDS             2266..2913
                     /locus_tag="VFX34_04250"
                     /inference="COORDINATES: protein motif:HMM:NF037496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEX5564149.1"
                     /translation="MIKSATLYTNNLKALRRFYVNILELDMTESTAEQFTVRIGESQL
                     TFKQSEQPAFYHFAINIPGNQFSMMKYWITDRLTLNREEGRDEVYFPSFDADSMYFED
                     PVGNIVELIGRRKRDLFGDLTKESFLNISEVGMATPHVVEVGDELQDLGIPLRNGTEV
                     EPDELNFLGRGDAFIVLVPLGRRWYFSKKVAESYPLELTWNDTHIKLKEDGRLTF"
     gene            3012..3455
                     /locus_tag="VFX34_04255"
     CDS             3012..3455
                     /locus_tag="VFX34_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010531965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564150.1"
                     /translation="MRKFLRLIGIGITSGIILAVLMQLVFAMTGNEAYILLYNVDYFP
                     LIHIFKGSASFGFAFHLVFCIASVVSLFYVLTFFEWQYSIWPYVAVYTAGSGALYFLT
                     MLTEKPPAADDEMAWFFWTASHFVFSIAVGLMINRFVKSETDARV"
     gene            3596..4744
                     /locus_tag="VFX34_04260"
     CDS             3596..4744
                     /locus_tag="VFX34_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HEX5564151.1"
                     /translation="MDFIWTLLFMALIGALIGGFTNHLAIKMLFRPHEAKYIGNWRLP
                     FTPGLIPKRRDELAVQLGRTVTNYLVTPDVLRNKLLTPEMERKAEAFLQSKFEEHVLQ
                     SDKTLHDWLQVAGATDIVEKAGRKIDALLDAQLVNVRTKLTSGTIEEIVPLEWQEQAK
                     AHIPAMTNYILQRAELFVDSYEGKVIFQKLINDFLESKGTFGGMLNMFFGDSNSLVEK
                     VQREALKFIKAPGTYDLLHSLIDKEFTKLQKRPMAELLGGFNWEGLFTSIKAYANKEL
                     ALNSRLDKTIQEYWPAGSDWTAENLTPKVTQFAFDQAQKQLEESLKKLKLDQIVKEQV
                     DTFPVSVLEDLVLGISRREFKMITVLGALLGGIIGIVQGLIVFFTNYS"
     gene            4757..5107
                     /locus_tag="VFX34_04265"
     CDS             4757..5107
                     /locus_tag="VFX34_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEX5564152.1"
                     /translation="MTVNIYDDLNKLESTLRKTDEYAEVKNAIEEVKKDEQALELFKS
                     FRKIQMTLQQKQMQGEEIAHEELEYAQKTAQLAQQNEKIMQMLQAEMKLSGLIEEVNR
                     VLMKPVQEMYESMQ"
BASE COUNT         1428 a         1095 c         1237 g         1390 t
ORIGIN      
        1 gaaccgtccg ccttcgacga gctcatcggg gaagcgcggt gtaattttgg acacgttctc
       61 agcgcagaac gggcattttt cgccttctgt cgcttttgcg agctccgaat aatccttcgg
      121 cataaatgga gcacccggat cgaaaatgac cctggaagtt tcccctgtca acggatcgaa
      181 tctgatttcc gtctttctgt caatcagctt cccatcttgc atcggatcat ggaatgtgaa
      241 gaattcttca tgtttgcgaa acgaaactgg catagttata gtccccctta aaaaggtctt
      301 tcttccattc tatcgttaac tgacagaaaa gtcagttgga tgaattatcg aagtgtcgtt
      361 ttgacgaaac cttttccatt attcacagtc acttttccgt acgccccatt cacgaacgta
      421 atttgcggag ggacatgtcc gacatctgca tcgtaaataa tcggcagtcc ggtcatctct
      481 tttatccttt ccatcgaatc aattacggtg aatccttcaa cttctttacc ggcaggggag
      541 cggccgaaga tgatgcctgc agcattctta aaccatcccg catagacaaa ctgaagcatc
      601 gtccggtgaa aatcggtagc attcatttca cagttctcga aataccataa aatcggctca
      661 tcgtccaaga agtttttctg gaatgaagcg atatccccgt atggcgttcc cacaagatgc
      721 cggatcgtat ccatacagcc ccctaaaagg cgaccttcca attcgatgtt agtagatccg
      781 atcactttcc attccgtcgg cgaatccagt ttatatacat atggatcatc cggagcgtca
      841 tgttgccatt ttgattggaa tttctcggat gaatgttgct caatgcttgt tcctgcaggg
      901 gcttgtaaga catccatgaa cttgcttgcc acaggctccc attcatcact gcgcatttct
      961 acgaagttcg gtccatgcgc ggtggcaatt ccggttttga cggtcaacgc aaataggaaa
     1021 gtgctcatat ccgaatagcc taaaatccat tttgggtaga tttcatccca atcaatgaga
     1081 ggcaatattt caatcaggat ttccccgccc cagggaggaa tgattgcatg gatttccgga
     1141 ttgttgaaca tggccataag ctctgctgca cgaacttctt tcgttgcaga agctgcctgg
     1201 atttgtgtcc ataccgtatc gccgacattc acttgataac ctcttttttc aaactggcgt
     1261 ttggcgttat ggattaaatg gtgaagggtt tcttccacac cagatgacgg tgctgtcacg
     1321 ccgattgtat ccccttgctg caatggtttc ggataacgga tcataagaat cactccattt
     1381 tctttcataa tttatggtag gatggttgta gagtagttta tcaagtattc ttaaaactgt
     1441 cactactatt taacggggat gattaatgat gaagcagggg gggttcatag tcaccttact
     1501 tgcaatgatg tttttgtccg gttgtggaca cagcgaaacg aatgcaatct atttactccc
     1561 tgagggctac acaggttccg caattgcggt atataacgtg gaaggtgcac cggtactgac
     1621 atatgaaggg gatttcgcag tctatcggat aaacgaagac ggttattttg caacgtcctt
     1681 gccggatatg caatatggaa ccgtgacgga tcgctattat tatgtggatc cggcaggtaa
     1741 ccggacacct atcgatccga attgcataca tctgtttgga actggtgcgg tttctacaga
     1801 taaagtggat cttgtatata caggaataga agttaccgac agttgttcag aggaatttgc
     1861 caacagcaca gaaaatttca gcgaagaacc gaagatggag attttgaata aagtcgcgaa
     1921 gaagttttat ggcgttggat tacaaccata aaaatgggga ggcggaatgg ctttgctgaa
     1981 atataaaaga atgtttttgc tgagtgtgtt gctcgctgct ttttccattt tctttttgcc
     2041 tcagaccgaa tatggtttcg gttggccaat tccttggttg gaggtacatg gcaataaaac
     2101 aatgattaca ggcagtgagc ttttacaagt gatgaatgta aagaacacat tctttgattt
     2161 atggaatttg ttattaggct ccctcattat ttatggcatg ctattcattg cctataaagg
     2221 attgaataaa ttaaataaag atacagagat aggggaaaag tgaagatgat caagtccgca
     2281 acattgtata caaacaatct caaagcgtta cgacggtttt atgtaaacat actggaactc
     2341 gatatgaccg agtctacagc tgagcagttc acagttcgaa tcggcgaatc gcaactaacc
     2401 ttcaaacagt cagaacaacc ggctttctat catttcgcaa tcaatattcc gggcaaccaa
     2461 ttctccatga tgaaatactg gataacggac cggctcacgt taaaccgtga agagggaaga
     2521 gatgaagtct acttcccgag ctttgacgcc gattcgatgt atttcgaaga tcccgtcggt
     2581 aatatcgtcg aattgatcgg ccgtagaaaa cgtgatcttt tcggagacct gacaaaagaa
     2641 tcgttcttga atattagtga agtcgggatg gcgacacccc atgtcgttga agtcggcgac
     2701 gagctgcaag acctcggaat accgctccgc aacggcaccg aagtggaacc ggacgagctg
     2761 aacttcctcg gacgtgggga tgcgttcatc gttctcgttc cgctaggccg tcgctggtat
     2821 ttttccaaaa aggttgcgga gtcatatcca ctcgaactga cgtggaacga tacacatatt
     2881 aaattgaaag aggatggtag gcttaccttc tagatggaac gtcgaatgcc gtgaacctgg
     2941 catgttcatg tgcaacaggg gaaagctggg gatgacatga aagggtatca agaacgtcgg
     3001 gaggagaacg gatgaggaaa ttcttgcgac tgattggaat cggaatcaca agcggaatca
     3061 ttttggctgt cctcatgcaa ctcgttttcg caatgacagg gaacgaagcg tacattttgc
     3121 tttataatgt agattatttt cctcttattc atatattcaa gggcagcgcc tcattcggct
     3181 ttgcgtttca tctagttttt tgcatcgcta gtgttgttag cctgttttac gtattaacgt
     3241 tctttgaatg gcaatacagc atttggccat atgtcgctgt gtataccgct ggcagtggag
     3301 cgctgtattt tttgacgatg ctaacagaaa agccgcccgc tgcggatgac gagatggcct
     3361 ggttcttctg gacagccagc cacttcgttt tcagcatcgc agtcggcttg atgattaacc
     3421 ggtttgtgaa gtcggaaacc gatgcaagag tatgaccatg tttacacact aaccgattac
     3481 ctatttgggt gatcggttct tttcatgcca gcggtcgccc caaaccgaat gctttttcca
     3541 caagcccggc ttctgttata attgaacccg aatgaatcgg aaataaggtg aagcgatgga
     3601 tttcatatgg actttgttgt ttatggcttt aatcggggcg ttgatcgggg gatttacgaa
     3661 tcacctggcg atcaaaatgc tgttcaggcc ccatgaggca aaatatattg gaaactggcg
     3721 tctacctttc acaccggggt tgattccaaa gcggcgtgat gaacttgccg tgcaactagg
     3781 gagaacggtc accaactatt tagtgacgcc cgatgtgctg cgcaataaat tattgacgcc
     3841 tgaaatggaa aggaaagcgg aagcattcct tcaaagcaag tttgaggaac atgtgctgca
     3901 atcggacaag acgttgcatg attggctgca agtggcaggc gcaaccgaca tcgtagagaa
     3961 agccgggcgg aagatcgatg ctctgctcga tgcacagctc gtcaacgttc gcacgaaatt
     4021 gacatcgggc acgattgaag aaatcgtccc gttggaatgg caggagcagg caaaagccca
     4081 tattcctgca atgacaaact atatcctgca acgggcagag ttgtttgtgg attcctatga
     4141 aggcaaagtg atatttcaaa aattgatcaa tgacttcctc gagtcgaaag ggacgtttgg
     4201 cggcatgttg aacatgttct tcggcgattc caactcgctc gtcgagaagg tccagcggga
     4261 agcgttgaag ttcatcaagg caccaggcac gtacgatctt ttgcattcat tgatcgacaa
     4321 ggaatttacg aagctgcaaa aacgaccgat ggcggaattg cttggcggat tcaattggga
     4381 aggacttttc acctccatca aagcatatgc taacaaggaa cttgcgttaa actcacgctt
     4441 ggataagaca atccaagaat attggccggc tggttccgat tggacagcag aaaatctgac
     4501 gccgaaagtt acgcaattcg cttttgatca agcacaaaag cagttagagg aatcattgaa
     4561 aaaactgaaa ctcgatcaaa ttgtaaaaga acaagtcgat acattcccgg tttctgtcct
     4621 tgaagatctc gtcctcggca tttccaggcg cgaattcaaa atgatcactg tccttggcgc
     4681 gctgttaggt ggtatcatcg ggattgtgca aggattgatc gtctttttca caaattactc
     4741 atagggggaa taaatgatga cagttaatat ttacgatgac ttgaacaaat tggagtcgac
     4801 acttcggaag accgatgaat atgcagaagt gaaaaacgcg atcgaggaag tgaagaaaga
     4861 tgaacaggcg ctggagctgt tcaagagctt ccgtaaaatc caaatgaccc ttcagcaaaa
     4921 gcagatgcaa ggcgaagaaa ttgcacatga agaattggag tatgcacaaa agacagcgca
     4981 attggcacaa caaaacgaaa agatcatgca aatgctccaa gcggaaatga agctgagcgg
     5041 cctgatcgaa gaagtgaacc gtgtcttgat gaagccggtt caggaaatgt atgaatccat
     5101 gcaataaaac cggctcagcc ggtttttttg tgttattatt tcagttagag
//
LOCUS       DASULI010000197         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1246151,
            whole genome shotgun sequence.
ACCESSION   DASULI010000197 DASULI010000000
VERSION     DASULI010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246151"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1296
                     /locus_tag="VFX34_04270"
     CDS             148..1296
                     /locus_tag="VFX34_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5564153.1"
                     /translation="MPRYRFETDEHDLFRKSLRKFLEKEAVPFYEEWEKARLVPTSFW
                     KKLGEMGFLCPQVDEQYGGLGLDFSFGVIIGEEMERVGSGLTGVGLHNDIVVPYLESY
                     GSDEQKKKWLPGCVTGDFITAIAMTEPGAGSDLAGIQTTAIRDGDHYVVNGQKTFITN
                     GINGNLFLVVVKTDPAAEPRHRGISLLMIEEGTPGFSKGRKLDKVGMHSQDTAELYFE
                     DCRVPVENLIGEEGKGFRYLMEKLQQERLVVALAAQTAMEDMLEMTIDYVKTRKAFGK
                     PVSSFQNTQFKLAEMATKVEIGKTFLESLIEDHLAGKDIVTKVSMAKYWLTETARDIS
                     VECMQLHGGYGYMEEYKIARRYRDIPVMSIYAGTNEIMKVIIAKNMGL"
     gene            1300..>2142
                     /locus_tag="VFX34_04275"
     CDS             1300..>2142
                     /locus_tag="VFX34_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5564154.1"
                     /translation="MKMLEGIKILDVTYYLPGPYASMRLAEMGADVIKVEPPEGDPAR
                     TLSGGVVHRANNKGKQFRFLDLKSEVGKEEMLELIRGTDVLIESFRPGVMERLGFGYK
                     TCAAYNPGLIYCSMTGYGQTGPIAECGSHDLNYMALSGLLSQLTDQQGHPIHPRNTIA
                     DYVGAMAVTEAILAALVQRGKTGKGAYLDVSITDALQAFQGTHRAYAEEGLSNRGIPE
                     IDGSYVSYGIYETKDKRHVVLAALEPKFWRNFCAFAERPDWLEHNQARIGSEIHREIE
                     QSFAA"
BASE COUNT          584 a          430 c          616 g          512 t
ORIGIN      
        1 agcaataaga tctgattcgt cgaattagct accgaaccct taaatacagg agtgggtcag
       61 ttgagtcaga cggagacagt cgaatacaga gatgtgtcag tgtctaccgt attggctgtc
      121 caaaaacaag agaatggggg attgttcatg cctcgatacc gctttgaaac agatgagcat
      181 gatttatttc ggaaatcact tcggaagttt cttgaaaagg aagctgttcc gttttacgag
      241 gaatgggaga aagcgcggct cgtccccaca tccttttgga agaagctcgg ggaaatgggg
      301 tttctctgtc cgcaagtcga tgagcaatac ggaggattgg gtcttgattt cagctttggc
      361 gttattatcg gcgaagagat ggagcgtgtc gggtcaggat tgactggagt tggcctccat
      421 aacgatatcg ttgtccctta tttagagtcg tacggttcag atgaacaaaa gaaaaaatgg
      481 ctgccaggtt gtgtaaccgg tgattttatc accgcgattg ccatgacgga accgggagcg
      541 ggctccgatt tggctggcat ccagacgacc gccattcgtg acggcgacca ttacgttgtc
      601 aacggacaga aaacgtttat tacaaatgga atcaacggaa atctctttct cgttgtggtg
      661 aaaaccgatc cggctgcaga accgagacat cgcggaatta gccttctcat gattgaagaa
      721 gggacgccag ggttctcaaa gggaagaaag ctcgataaag tcgggatgca ttcgcaggat
      781 acggccgagc tttactttga agattgccgg gttccagttg agaacttgat tggcgaagaa
      841 ggaaaagggt tccgctatct catggaaaag cttcaacagg agcggctggt cgtggcgctt
      901 gctgcacaaa cagcaatgga agatatgttg gagatgacga tcgactatgt gaaaacgcga
      961 aaggcgtttg gcaagccggt tagttcattc caaaacacgc agttcaaact ggcggaaatg
     1021 gcgacgaaag tggagattgg aaaaacattt ttggaatcgc tcattgaaga tcatctggct
     1081 gggaaagaca tcgtgacgaa ggtgtcgatg gcaaagtatt ggctaacgga aacagcgcgg
     1141 gacatttcgg tcgagtgcat gcagttgcac ggcgggtacg gctacatgga agaatacaag
     1201 attgccagac ggtaccggga tattccggtc atgtcgattt atgcaggaac gaatgaaatt
     1261 atgaaggtca tcattgcgaa aaacatgggg ttgtagcaaa tgaagatgct tgaaggaatc
     1321 aagattctcg acgttacgta ctatcttccg ggaccttatg cttcgatgag gttggcggag
     1381 atgggagcgg atgtgatcaa agtggagcca ccggagggag accctgcccg tacattaagc
     1441 ggcggggttg tccaccgtgc aaacaataaa ggcaagcagt ttcgtttcct tgatttgaaa
     1501 tctgaagtag ggaaggagga gatgttggaa ctgatccggg gaacagacgt gttgattgag
     1561 tcgttcagac cgggagtcat ggagcgcctc ggatttggct ataaaacgtg tgctgcatac
     1621 aatcccggac tcatctattg ttccatgacc ggttatggcc agacaggtcc gatcgcagaa
     1681 tgcggcagcc atgatttgaa ctacatggcg ttatcgggac ttctctcgca attgacggat
     1741 caacagggac atcctattca cccacgtaat acgattgcgg attacgtcgg ggctatggcg
     1801 gttacagaag ctattctcgc tgcccttgtc caaaggggga agaccggaaa aggtgcctat
     1861 ctcgatgtgt cgataaccga tgcgcttcaa gcattccaag gcacccatcg tgcatatgct
     1921 gaagagggat tgtctaatcg gggaattccg gaaatcgatg gatcctatgt ttcgtacgga
     1981 atctatgaaa caaaggacaa acgacacgtt gttctcgcag cgttagagcc aaaattttgg
     2041 cggaacttct gtgcatttgc ggagcgtccg gattggttgg agcataatca agcccggata
     2101 ggaagtgaga ttcataggga aattgaacaa tcttttgcgg ct
//
LOCUS       DASULI010000198         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_681302, whole
            genome shotgun sequence.
ACCESSION   DASULI010000198 DASULI010000000
VERSION     DASULI010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681302"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1146
                     /gene="pheS"
                     /locus_tag="VFX34_04280"
     CDS             109..1146
                     /gene="pheS"
                     /locus_tag="VFX34_04280"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5564155.1"
                     /translation="MEQQLQQLKEEALTKIQEATTVKELNDVRVAYLGKKGPITDLLK
                     GMGKLPAEERPKMGALVNVVREAVTESLEAKMTKLEEEAINAQLEKEAIDVTLPGRPV
                     PTGNHHPLTRVVEEIEDFFISMGYEIAEGPEVEKDYYNFEALNLPKGHPARDMQDSFY
                     ISEEVLLRTHTSPVQARTMEAKGGSAIKIICPGKVYRRDNDDATHSHQFTQIEGLVVG
                     EDIRMSDLKGTLDLFAKKMFGSDREIRLRPSFFPFTEPSVEMDISCFKCGGDGCNVCK
                     KTGWIEILGAGMVHPNVLKMAGYDPSVVTGFAFGMGPERIAMLKYGVEDIRHFYTNDV
                     RFISQFHRTEA"
     gene            1160..3571
                     /gene="pheT"
                     /locus_tag="VFX34_04285"
     CDS             1160..3571
                     /gene="pheT"
                     /locus_tag="VFX34_04285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5564156.1"
                     /translation="MLVSTKWLNEYVNINGINPADLAERITRSGIEVDSIIDRSQGMT
                     NIVVGHVVECVKHPEADKLNICQVNVGEETTQIICGAPNIAEGQKVIVARPGAVLPGG
                     MKIKKAKLRGEESNGMICSLQELGIEGKLVPKAYAEGIYVLPQDVLPGENALPLLGLD
                     DTVLEFDLTPNRSDALSMLGVAYEVSAILSQGIALPKIDYPTATEKAEDVLKLRVDAH
                     ADNPMYVAKVVKNIKVAESPLWLQNALMASGVRPHNNVVDITNYVLMEYGQPLHAFDY
                     DRLETGEIVVRHAEEGEMMTTLDDAERKLQSHNLVITNGKEPVALAGVMGGANSEVHD
                     GTTTVVIESAYFAPSSVRRTSKEVGLRSDASTRFEKGVDPNRVQEAAERAAQLIAELA
                     GGEVLAGSVIFDELDKTAERITVSPDYINSRLGMKISMDEMLTILERLRIPTQAINGQ
                     LVIDAPTRRQDLKIREDIIEEIARMHGYDEIPKTLPVSESTPGGLTPYQAKRRIVRQF
                     LEGAGLYQAVTYSLTSKEQAQAFALETAPVTELLMPMSEDRSILRQSLIPHLLEAATY
                     NVARRIDSVALYETGSVFLGEEEDGLPREVEHVAAVLTGKWVDHPWQAETKKVDFFVV
                     KGIVEGMMDLLGLLDGLSFEKTVVEGMHPGRTANVLLHGERIGLIGQIHPTEQKKRDL
                     KETYVMELNLKAILSVETDELLYVPVPRYPSISRDIALVVASETSAGTLEGIIKQAGG
                     KLLKDVKLFDLFEGDNVEEGKKSLAFSLTYFDPERTLTDEEVVKAHEKVLNALTAEAA
                     AQLRG"
BASE COUNT         1056 a          837 c         1057 g          941 t
ORIGIN      
        1 ccaaatgaag tgattatgcg tttctgcata ataatcaggg tggtaccgcg aactcgtcct
       61 cgtccctttt tgggggcggg gcttttttat tttgaaagga gaatgacaat ggaacagcaa
      121 ttgcagcaat tgaaagaaga agcgttgacg aaaatccagg aagcaacgac tgtcaaagaa
      181 ttgaatgacg tccgtgtcgc ttacctcggc aagaaaggtc ccattaccga tttattgaaa
      241 gggatgggga aactgcctgc tgaagagcgt cctaaaatgg gtgctctcgt caatgtcgtc
      301 cgtgaagccg tgacggaatc actggaagcg aaaatgacga aactcgaaga agaagcgatc
      361 aatgcacaac tggaaaagga agcgatcgac gtcacattgc ctggccgacc tgtaccaact
      421 ggcaatcacc atccgctgac acgcgttgtt gaagagatcg aagacttctt catcagcatg
      481 ggctatgaaa tcgcggaagg accggaagtt gaaaaggatt attataactt tgaagcgctg
      541 aacttgccga aagggcatcc ggcgcgtgac atgcaggatt ccttctatat atccgaagag
      601 gtccttcttc gcacacatac gtcgccggtc caagcgagaa cgatggaagc taaaggcgga
      661 agtgcgatca aaatcatctg cccgggcaaa gtgtatcgcc gggataatga cgatgcaaca
      721 cactcccacc aattcacgca aatcgaaggg cttgttgtcg gtgaggatat ccgaatgagc
      781 gacctgaaag ggacgcttga cctttttgcg aagaaaatgt tcggctccga ccgtgaaatc
      841 cgattgcgtc ctagcttctt cccattcacg gagccgtctg tagagatgga tatctcttgc
      901 ttcaaatgtg gcggggacgg ctgtaatgta tgcaagaaga caggttggat tgaaattttg
      961 ggagcaggca tggtacatcc aaatgtgctg aagatggctg gctacgaccc atccgtcgta
     1021 actggatttg cattcggcat ggggcctgag cggattgcga tgctgaaata tggagtggaa
     1081 gatatccgtc atttctatac gaatgacgtt cgtttcatat cacaattcca tcggacggaa
     1141 gcttgaggag gagattacat tgttagtatc aacaaaatgg ctaaatgagt atgtcaatat
     1201 aaacgggata aatccagcgg acttagcgga aaggattaca cgttcaggca ttgaggtgga
     1261 ttccatcatc gaccgttcgc aaggcatgac gaatatcgtt gtcggacatg tggtggagtg
     1321 tgtgaagcat ccggaagcgg ataaattgaa catatgccaa gtcaacgtcg gggaagagac
     1381 aacacaaatc atttgtggag cgccgaatat cgcggaaggt caaaaagtga ttgtcgcgcg
     1441 accaggtgct gttcttccag gcggcatgaa aatcaaaaaa gcgaaacttc gcggcgaaga
     1501 atcgaacggg atgatctgtt ctttgcaaga gcttggaatt gaagggaaac tcgttccgaa
     1561 agcttatgca gaaggtatat atgtattgcc tcaagatgta ttgccgggtg aaaatgcact
     1621 gccattgctt ggcttggacg atacagtgct tgagttcgac ttgacgccga atcgctcgga
     1681 tgcgctaagc atgttagggg ttgcttatga agttagcgca attttatcac aaggaattgc
     1741 cttgccaaaa attgattatc ctacagcgac tgaaaaagcg gaagacgtgc tgaagctccg
     1801 tgtggacgcg cacgctgata atccgatgta tgtagcgaaa gtcgtgaaga atatcaaagt
     1861 ggcggaatct ccattatggc tgcagaatgc tttgatggct tcaggcgtcc gtcctcataa
     1921 taatgtagtc gacattacga actacgtatt gatggaatat ggccaaccgt tgcacgcgtt
     1981 tgactatgac cgacttgaaa ctggcgaaat cgtcgtccgc cacgctgaag aaggtgaaat
     2041 gatgactacg ctggatgatg ccgaacggaa attgcaatcg cataaccttg tcattacgaa
     2101 tggtaaagag cctgtcgctt tggccggtgt catgggtggg gcaaattcag aggtccatga
     2161 tggaacgaca actgtcgtca ttgaatccgc ttatttcgct ccttcatcag ttcgccgcac
     2221 ttccaaggaa gtcggactac gtagtgatgc aagcacacgt ttcgaaaaag gcgttgaccc
     2281 gaaccgggtt caggaagcgg cagaacgtgc agcacagctt attgctgaac tagcgggcgg
     2341 ggaagtcctt gcgggatcgg tcatctttga cgaacttgat aaaacggctg aacgcataac
     2401 agtttcgcct gactatatca acagccgcct aggcatgaaa atatcgatgg atgaaatgct
     2461 caccattttg gaacgcctcc gtattccgac gcaagcgatc aatggccagc ttgtcatcga
     2521 tgcaccgaca cgccggcagg atttgaagat cagggaagac attatcgagg aaatcgcgcg
     2581 tatgcatggt tatgacgaaa ttccgaaaac acttcctgtt tccgaatcga cgccaggcgg
     2641 attgacgcca taccaggcga agcgccggat tgtccgccag ttccttgaag gagcgggact
     2701 ttatcaggca gtcacgtatt cattgacatc aaaggagcag gcgcaggcat ttgcactgga
     2761 aacagctccg gtgacagaat tgcttatgcc gatgagcgaa gatcggagca tcctccgaca
     2821 aagcttgatt ccacatcttt tggaggcggc tacgtataac gttgcacgtc gtatcgattc
     2881 agtcgcgctt tacgaaacag gctcagtttt cctcggggaa gaagaggacg gccttccgcg
     2941 tgaagtggag catgtggcgg ctgtcttgac aggcaagtgg gtcgatcacc cgtggcaagc
     3001 agagacgaag aaagttgatt tcttcgttgt gaagggaatt gtagagggca tgatggatct
     3061 tcttggtctg cttgatggct tgtcatttga aaagactgtg gtagaaggta tgcatcctgg
     3121 acggacggct aacgtgctat tgcacggaga gcgcattggc ttgattggtc aaatccatcc
     3181 gaccgaacag aagaaacggg atctgaaaga gacgtacgtg atggaattga atctgaaagc
     3241 gatcttatct gttgaaacgg atgagctgct atatgtgcct gttccgcgtt acccttctat
     3301 ttcacgggat attgcactcg tcgttgcaag cgaaacttcc gctggtacgt tggaaggcat
     3361 catcaaacag gcgggcggca aactgttgaa agatgtgaag ctcttcgatt tatttgaagg
     3421 ggacaatgtt gaggaaggca agaaatcact cgctttctca ttgacgtatt tcgacccgga
     3481 gcgtacgttg acagacgaag aagtcgtcaa agcacatgaa aaagtattga atgctttgac
     3541 tgcagaagca gccgcacagc ttcgcggata aaacgaaaga ccgcttatgg ggattggttc
     3601 tccataagtg gtcttttttg tgtcgttgtc caaaatgttt gtattcaggc ggtcgcggtg
     3661 caacattagg cggttcgacc aggttattag gcggtcgtag tgcagcatca ggcggtcaga
     3721 cagagttttt aggcggtctc aatacaacct cgggcgatgc gaactagata tccgaggcgt
     3781 ttcgagttca aagcaatcac cttttcatcg ctgcaatctt tttcgctttc tcggcattgg
     3841 caaaatgcca tttcgtcatc gatttcaacg tcgcttctcc atttttcaat a
//
LOCUS       DASULI010000199         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_272967, whole
            genome shotgun sequence.
ACCESSION   DASULI010000199 DASULI010000000
VERSION     DASULI010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272967"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VFX34_04290"
     CDS             complement(<1..1083)
                     /locus_tag="VFX34_04290"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5564157.1"
                     /translation="METGYLFSSLPIKKVEGVLPDTVTDIAVDSRSVNAGGVFVCIKG
                     FTVDGHAFVPKAIDNGARVIVASRPIEVNLDKVAVVYVEDTARAIALLAPRFMNYPSK
                     RMTMIGVTGTNGKTSVSNMLHSVLRHAGEKSAVTGTIGFNLDGVLFETENTTADVLTT
                     QQMIKQAADAGCSTMTMEVSSHGLIEGRLAGTEFDIAIFTNLSHDHLDFHGTMENYGH
                     AKGLLFAQLGQDLEQSKFGVVNVDDPWGVRMKEMTSFPVWTYGVHNEAHFRAMDVKLT
                     VDSTSFYLDSPAGGFDVKMSLIGEFNVYNALAVIAALYAKGLEMEVILEGLAVIPSAK
                     GRMEKVETELPLTIYVDYAHSPDAIEK"
     gene            complement(1282..2331)
                     /locus_tag="VFX34_04295"
     CDS             complement(1282..2331)
                     /locus_tag="VFX34_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX5564158.1"
                     /translation="MHQFNESRTIELLKRLVETPSPSGFTEKVMQVIGDELGEIGVPF
                     KRTNKGAVVATLKGKDDSRHRLLTAHTDTLGAMVKEIKSNGRLKLTMIGGFNWNAVEG
                     EYCHIHTASGKEVRGTILMHQTTVHVYRNSGSAERNADNIEVRIDEKVKNAAETRALG
                     IEVGDFVSFDPRFEVTPAGFVKSRHLDDKASTALLLELVRSLKENGTELPNTTHFYIS
                     NNEEIGYGGNSNIPDETVEYIAVDMGAIGDGQSSDEYTVSICAKDSSGPYHYALTRHL
                     TALCEKGSVPYKLDIYPYYGSDASAAIRAGFDVRHALFGPGIESSHALERTHVDSLKA
                     TAQLLHAYVLSEMLK"
     gene            2429..4039
                     /locus_tag="VFX34_04300"
     CDS             2429..4039
                     /locus_tag="VFX34_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5564159.1"
                     /translation="MENGHPVQSKNKYLIISFGFIVFIQLISSPFFDNAAYLLPIAAA
                     AVFNLSLLGLLHYSKIDQKIISWLIIFGSNFYILSLLFVNSDFPYLFFLFFPIIASLV
                     YSDKYLHITMLIVTTIELTVILSVLGPYFHPIRDAKMVIHYSFLFAIVMTLLFIFTMK
                     IGPEMKKLMIEKEVMSATLSSKEGYLDLFFEHAQDAIAVFTLDQKLLAVNPAFEEMYG
                     WKKDECIGKSIQLTPSSEFEGIAERQKYLLQGRSFKNVRSKELRKNGTVFDAETTVAP
                     IYDKNKEIIALSLITRDITERLQAERLRIEAIKLNAIGEIAATVAHEVRNPMTSVNGF
                     VQLMNEDPENPYRAYTEIMESEIDRINLIANEFLILSKPNIKQRREIDVENKIIEVID
                     LFEEELKIRQINCSLYLAEYPTTVFGNEESLKQVFINLMKNSIEAVPDGGNIMFFNSI
                     VNNMISITLSDTGHGMDEEIFKQLFMPFFTTKEGATGLGLVITKKIVLDHGGNIEFNE
                     LQRHGTELTVKFPIYSLNKYKDLTKAME"
     gene            complement(4112..>4365)
                     /locus_tag="VFX34_04305"
     CDS             complement(4112..>4365)
                     /locus_tag="VFX34_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX5564160.1"
                     /translation="QEYFLEHLSFGGGCINDTISHVGNIHLPFGGVGASGMNAYHGKA
                     SFDLFTHSKSILKKSTKVPIRLGYPPYGNTLQIVKPLLR"
BASE COUNT         1185 a         1048 c          895 g         1237 t
ORIGIN      
        1 cttttcgatc gcatcaggtg aatgagcgta atcgacatag attgtcagcg gcaattccgt
       61 ttcaactttc tccatcctgc cttttgcgga aggaatgacc gctagccctt caaggatgac
      121 ttccatttca agtcctttcg catataaagc tgcaataacg gcgagtgcat tgtatacgtt
      181 gaattcaccg atcaatgaca ttttcacatc gaagccgccg gcaggcgaat ccaagtaaaa
      241 ggaagtgctg tcaacagtaa gcttgacatc catcgcacgg aaatgcgctt cgttgtgaac
      301 accatatgtc cataccggaa aagaggtcat ttccttcatc cgcacccccc atgggtcgtc
      361 cacattgaca acgccgaact tcgactgttc cagatcttgt ccaagctgcg cgaataaaag
      421 cccctttgca tgcccgtaat tttccatcgt cccgtggaag tccaaatggt catgcgataa
      481 gttcgtgaaa atcgcaatat caaattcggt tcccgccaat cggccttcga taagcccatg
      541 tgaagatact tccatcgtca tcgtcgaaca tcctgcgtca gctgcctgtt tgatcatttg
      601 ctgggttgtc agcacatcgg ccgttgtatt ttcagtttca aataaaacgc catccaaatt
      661 gaatccgatt gtccccgtca cagcagattt ttcgcccgca tggcgcaata cggaatgtag
      721 catgttgctg acgctcgttt ttccgtttgt ccctgtcacg ccgatcatcg tcatccgttt
      781 ggaaggatag ttcatgaatc ggggagcaag caaggcgatc gcccttgccg tatcttccac
      841 gtagacgacc gcaaccttgt ccaagttgac ctcgatcgga cgggacgcca caatcactct
      901 tgcgccattg tcaatcgctt ttggaacgaa tgcatgtcca tccacggtaa accctttgat
      961 gcagacaaag acgcccccag cgttaactga gcgtgaatct acggcgatgt ctgtaacagt
     1021 atcaggtaat actccttcca ctttctttat tggcaaactt gaaaataaat aaccggtttc
     1081 catcaccagt cctcctctta ttgtctaact tcagacgctt aacggatacc ttaatttccg
     1141 caaaaaccgc agaaatacgg caaatcgaac ccttcgcagt tcgattcgct cgggtcataa
     1201 gtcaatttta ctttgtggca aaaggcgcca cgacgcaacc ttgccttata cctgtcgcat
     1261 cttagcaagc gtgttacgct tttacttcaa catttctgat aatacatatg catgcagtaa
     1321 ttgtgctgtc gctttcaatg aatcgacatg agtcctctcc agcgcatggg acgattcgat
     1381 tccaggaccg aacaatgcat gccgcacgtc aaaaccggcc cggattgcag cggatgcatc
     1441 agacccatag taagggtaaa tatcaagctt gtatgggaca ctcccttttt cacagagggc
     1501 tgtcaaatgc cttgtcaaag cataatgata aggtccgctt gaatctttcg cgcaaatgga
     1561 taccgtgtac tcatcggatg attgtccatc accgattgct cccatatcga ctgcaatata
     1621 ttcgaccgtc tcgtcaggga tattggaatt cccgccataa ccgatttctt cattgtttga
     1681 gatgtaaaaa tgggtcgtgt tcggtagttc cgtcccgttt tccttcaatg aacggaccaa
     1741 ttccaataga agtgcagtgc tcgctttatc atcaagatgt cttgatttta caaatccggc
     1801 cggtgtcact tcaaatcttg gatcgaatga aacgaagtct cccacctcaa tgccgagcgc
     1861 cctcgtttcg gctgcattct tcactttttc atcgatacgc acctcgatat tgtccgcgtt
     1921 ccgttcagcg cttccagaat tgcggtacac atggactgtc gtctgatgca tcaaaatcgt
     1981 acctctgact tccttgccgg aagccgtatg tatatggcaa tactcgcctt ccacagcatt
     2041 ccagttaaat ccgccgatca tcgtcagttt caatcttcca ttgcttttga tctctttcac
     2101 catcgctcca agcgtatccg tatgggcggt taacagccga tgtcttgaat catctttccc
     2161 cttcaaagtc gcgacgaccg cacctttatt cgtccgcttg aaaggaactc caatttcacc
     2221 aagttcatca ccgatgactt gcatcacttt ttccgtaaag cctgatggac ttggcgtttc
     2281 cactagcctc ttcaataatt cgattgtccg tgactcatta aattgatgca tcttccattc
     2341 cccttttcct ttttctgaaa tttccgtttc cattgtaaat caaatgatgt atgatactta
     2401 taatactact actttctgag gtgtatcaat ggaaaacggt catcctgtcc aatcgaaaaa
     2461 taagtatcta atcatttcgt tcggttttat agttttcatt caactgatct cttcgccctt
     2521 ctttgacaat gcggcttatt tattgccaat cgcagccgcg gcagtattca acctaagtct
     2581 tcttgggctt ctgcattata gcaagattga ccaaaagatc atcagctggc tgatcatttt
     2641 tggaagcaat ttttatatcc tttccttgtt gttcgtcaac tcagactttc catatctctt
     2701 ctttttgttt ttccctatca ttgcatctct cgtctacagt gataaatact tgcacattac
     2761 catgttaatt gtaacaacga ttgaactaac agtaatatta agtgtcttag gtccatattt
     2821 ccatcctatt cgagacgcaa aaatggtcat ccactacagt ttcctcttcg ccatcgtcat
     2881 gacgctgctc ttcattttca ccatgaagat cggaccggaa atgaaaaagc tgatgattga
     2941 aaaagaagtg atgagcgcaa ctctatcatc aaaagaaggc tatctggatc tgtttttcga
     3001 acatgcacaa gacgcgattg ctgtattcac tctcgaccaa aagctgctcg ccgtgaatcc
     3061 tgcattcgaa gagatgtacg gatggaaaaa agacgagtgc atcggtaaat ccattcaatt
     3121 gacaccttca tcagaatttg aaggtatagc agagcgtcaa aaataccttc tgcaagggag
     3181 aagctttaaa aatgtccggt caaaggaact tcgtaaaaac gggacagttt ttgatgcaga
     3241 aacaacagtt gcaccgatct atgataaaaa caaggaaatt atcgcattgt ccctcatcac
     3301 gcgcgacatt acggaaagac tgcaagcgga gcgactaaga atagaagcca taaagctaaa
     3361 tgcaatcgga gaaatcgctg cgactgtcgc ccacgaagtg cggaacccga tgacgtccgt
     3421 caatggattt gtgcaattga tgaatgaaga tccggaaaat ccttatcgcg cctatacaga
     3481 gattatggaa agtgaaatcg atcggatcaa tttgattgcg aacgaatttt tgattttatc
     3541 gaagccgaat attaagcagc gaagggaaat agacgttgaa aataagatca ttgaagtcat
     3601 cgatcttttt gaagaggagt tgaaaatacg acagatcaat tgctcgctct atctagcgga
     3661 atatccgacc actgttttcg gcaacgaaga aagtctgaag caggtgttta tcaatttgat
     3721 gaaaaattca atagaagcgg tgccggacgg agggaatata atgtttttca actccatcgt
     3781 caataatatg atttccatta cgttgagcga tactggacac gggatggatg aggagatttt
     3841 caaacaattg ttcatgccgt ttttcacaac gaaggaaggg gctacgggac tcgggcttgt
     3901 tatcacaaaa aaaatcgtgc tggatcacgg cggtaatatt gaatttaatg agctgcaaag
     3961 acatggtacc gagttgaccg tgaagttccc gatttactca ttgaacaaat ataaggacct
     4021 tacgaaggca atggaatgac ggccgacaat tttaccatac taaatgaggc gaaggaacat
     4081 gcgatgcatc ccttcgcctt atctatggct cttatcttaa aagaggcttt acgatctgca
     4141 gtgtattgcc gtaaggcgga taaccaaggc ggatcggcac ttttgtgctt ttctttaaaa
     4201 ttgatttcga atgggtgaac aggtcaaagc ttgctttccc atggtatgcg ttcatgcctg
     4261 atgctccgac tcccccaaaa ggcagatgga tatttccgac atgggaaatt gtatcattta
     4321 tgcagcctcc gccgaatgat aagtgttcca agaaatattc ctgcg
//
LOCUS       DASULI010000200         5299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_673576, whole
            genome shotgun sequence.
ACCESSION   DASULI010000200 DASULI010000000
VERSION     DASULI010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673576"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..608
                     /locus_tag="VFX34_04310"
     CDS             366..608
                     /locus_tag="VFX34_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HEX5564161.1"
                     /translation="MRFSDLQKKEVIEVKKGSFLGFVQDATIDMKNGKIDVLQIGGAE
                     RALFLESKMKDLKQIKYDDVLTIGKDIVLVGKKTDK"
     gene            680..1375
                     /locus_tag="VFX34_04315"
     CDS             680..1375
                     /locus_tag="VFX34_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5564162.1"
                     /translation="MDTLQQRMRKIEEEIQAACDRSGRKRQDVTVVAVTKQVSATRTQ
                     EVIDIGITDIGENRPEGLLAKRESIQSDNLDWHFIGNVQSRRVRDIINEVDYLHSLDR
                     LSIAKEIQKRAASPVDCFVQVNVSGEESKSGLQPEELEDFVLQLAEYDNIRVVGLMTM
                     APFTQDEEWIRKIFRSLRDLRDQLAAKEMEHAPCTLLSMGMSNDFIIAIEEGATHVRI
                     GTALVGTESEGDE"
     gene            1372..1824
                     /locus_tag="VFX34_04320"
     CDS             1372..1824
                     /locus_tag="VFX34_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX5564163.1"
                     /translation="MSIKRRFEKWFYLDEEEEFQQGQPVREQQPVQEPQQTRRAVSRK
                     PQVAENQQQPGTVVSLQSIQKSSKVVLFEPRVYAEAQDISEHLKNKRAVVVNLQRIER
                     DQGIRIVDFLSGTVYALGGDIQRIGTDIFLCVPENVEVAGTISDFFER"
     gene            1844..2140
                     /locus_tag="VFX34_04325"
     CDS             1844..2140
                     /locus_tag="VFX34_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5564164.1"
                     /translation="MELAAIITLLFKFLLNVIDIYMILLIVYILMSWVPASRETKFGR
                     FLGRITEPYLGFFRKFIPPLGMIDISPIVAIFVLNYLIRRGVGEVYYLIIASLT"
     gene            2168..2944
                     /locus_tag="VFX34_04330"
     CDS             2168..2944
                     /locus_tag="VFX34_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5564165.1"
                     /translation="MDSIIQHFRKDEQPFIETVIGWTREVEDTYSPKLTGFLDPRQIF
                     ILSSIVRSSGLQFGVHGAFLEPERQRALIYPDYYEPEAEDFQVSVFGIRYPAKFMTLE
                     HRDVLGSLMALGIDRSKFGDIRLEGDVAQFAAAEEMKDYLTANLTSIGKSKVRVEELG
                     ASDELIVIAESWKEELHTISSLRLDTLVASLVNCPRQKAAALIQNDKVKVNHVVRDQQ
                     AFEVNESDILSIRGSGRFKIMSIEGRTRKDKIRLLIGRLE"
     gene            3039..3545
                     /locus_tag="VFX34_04335"
     CDS             3039..3545
                     /locus_tag="VFX34_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX5564166.1"
                     /translation="MALSPLDIHNKEFSRGFRGYDEDEVNEFLEQIMKDYENVLDENK
                     TLKNSLKETKEKISHFNTIEETLQKSILIAQEAAEDVRRNSVQESKLIVKEAEKNADR
                     IVNEALSRARKIAMDIEDLKKQSKVFRNRFRMLVEAQLDMIKTDDWEKLLEYEPDTEH
                     LESAADVE"
     misc_binding    3602..3814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3855..>5299
                     /gene="ileS"
                     /locus_tag="VFX34_04340"
     CDS             3855..>5299
                     /gene="ileS"
                     /locus_tag="VFX34_04340"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5564167.1"
                     /translation="MDYKDTLLMPKTEFPMRGNLPNKEPDMQAKWEEMNIYEKVQKRT
                     EGRPFFVLHDGPPYANGDLHMGHALNKVLKDMIVRHKSMTGFHAPYVPGWDTHGLPIE
                     QALVNKGVNRKEHSVAEFRKMCEEYAYSQIDSQRTQFKRIGVRGNWENPYITLKPEFE
                     ARQIQVFGEMAKKGYIYKGLKPVYWSPSSESALAEAEIEYKDKKSPSIYVAFPIKDGL
                     GVVDEDVRIIIWTTTPWTIPANLGISVHPEFTYAIVNVDGNKFLLAKDLVEFVAKEIG
                     WETYEIVKELKGSELDRVIAKHPLYDRDSLVMLGEHVTAEAGTGCVHTAPGHGEDDFY
                     VSKQYGIDALSPINDRGVMTEEAPGFEGLFYEDANKEVTKKLEEEGALLKLSFITHSY
                     PHDWRTKKPVIFRATAQWFASIESFRDELLEAIRNTKFTPTWGETRLYNMVRDRGDWC
                     ISRQRVWGVPIPVFYAENGEAILTDETIAHVA"
BASE COUNT         1576 a         1066 c         1350 g         1307 t
ORIGIN      
        1 aatcgaacca cgaagagttc gatatgctta tttctgcgct ttttttgcag aaatttaggc
       61 atccatccgg ctcgtaacgc ccagctatta ctcgcaaaag ccgttctttg taacagcttt
      121 cgcaggagtc tcgtacttgc acttcaagca tcagctacaa agtaatagaa atttttttat
      181 gtaaagttac tgtcttttaa tttgttgatt gtaacggaag gcggcgactc ctgcgggaaa
      241 gcgggacagg ggagaccccg caggtagcaa agcgtagcgt agcggacgag gaggctcacg
      301 cccgcccgtg gaaagcgtcc gcctgaagtg gaaatcaatt tatatttatt agaggaggga
      361 ttactatgcg tttttcggat ttgcagaaga aagaagttat cgaagtaaag aagggctcgt
      421 ttctaggatt tgtacaggac gcgacaattg atatgaaaaa cggtaaaatt gacgtgttgc
      481 aaattggcgg agctgaaaga gccctgttcc tggaatcaaa aatgaaggat ttgaaacaaa
      541 tcaagtatga tgacgtcctt acaatcggga aagacatcgt attagtaggt aaaaagactg
      601 ataaatgaag acttatcatt gatatgtgtc catccaattg atacaataag gcaaagtaag
      661 cagaaaagta gtgatttcat tggacacact tcaacaacgt atgcgaaaaa tagaagaaga
      721 aattcaagcg gcttgtgacc gttcaggaag gaaacgccag gacgtaactg ttgtggctgt
      781 gacaaagcaa gtatccgcca caagaactca ggaagtcatt gatattggca ttacggatat
      841 tggtgaaaac cggccggagg gactcctcgc caagcgggag agcattcagt ccgataatct
      901 tgactggcat ttcatcggca atgtgcagag cagaagggta agggatatta ttaatgaagt
      961 cgattatctt cattcactgg accggctcag cattgcaaaa gagattcaaa aacgggcagc
     1021 atcaccggtt gattgcttcg tgcaggtgaa tgtttccgga gaagagtcaa aatcgggcct
     1081 tcagcctgaa gaattggagg atttcgtcct tcaattggcc gaatatgata atatccgtgt
     1141 cgtcggtttg atgacgatgg cgccttttac gcaggatgag gaatggatcc gaaagatttt
     1201 ccgttcattg cgcgaccttc gtgaccagct ggctgcgaaa gaaatggaac atgcaccatg
     1261 cacactatta tccatgggaa tgtcaaatga ttttatcatc gcaatcgaag aaggcgcaac
     1321 tcacgttaga atcggaaccg cattagtcgg aacggagagc gagggagacg aatgagcata
     1381 aaaagacgat ttgaaaaatg gttctatctt gatgaagaag aggagttcca gcaagggcag
     1441 cctgtcaggg aacaacagcc tgtacaagag ccacaacaga cgagaagagc ggtttcacgt
     1501 aagccgcaag ttgcggagaa tcagcagcag ccgggaacgg ttgtcagctt gcaaagcatc
     1561 cagaagtctt caaaagtcgt tctttttgaa ccgcgtgtct acgcagaagc gcaggacatt
     1621 tcggagcatt taaagaacaa aagagcggtt gtcgtcaatt tacagaggat cgagcgcgat
     1681 caagggatac gcatcgtcga tttcctcagc gggaccgtct atgcacttgg cggggatatt
     1741 cagcggatcg gcactgacat tttcctatgc gtgcctgaga acgtcgaagt ggctgggacc
     1801 atttcggatt tttttgaaag atagtattgg atgaggtgta cttatggaat tagcggctat
     1861 aataacgtta ttatttaaat ttttgctcaa tgtaatagat atttatatga tactccttat
     1921 cgtatatata ttgatgtcat gggtacctgc atcgagagaa acgaaattcg gaaggttttt
     1981 aggaagaatt actgagccat atttagggtt cttccgtaaa tttatccctc cgctcggaat
     2041 gatcgatatt tctccaattg tggccatctt tgttttgaat tatttaattc gaagaggagt
     2101 aggcgaagtt tattatttaa ttattgcctc actgacgtga ggttttttca ataaggagca
     2161 gttgacgatg gattcgatca tacaacattt cagaaaagac gagcagccgt tcatcgaaac
     2221 ggtgattggg tggacacgtg aagttgagga cacttattcc ccgaagctta ccggttttct
     2281 cgatccaagg cagattttca ttttgagctc aatcgtcaga agttcgggtc tgcaattcgg
     2341 ggtccatggc gcattccttg aacccgaacg gcagcgggca ttgatttatc ccgattatta
     2401 cgaaccggaa gctgaagatt tccaagtatc cgtttttgga ataagatatc ctgcgaaatt
     2461 catgacgctt gagcaccggg atgttttagg ttctctcatg gcgcttggca tcgaccgttc
     2521 caagttcggt gatatccgct tggagggaga tgtcgcccaa ttcgccgccg cggaggaaat
     2581 gaaagattat ctgaccgcta atttgacgtc tatcggaaaa tcgaaagtcc gtgtcgaaga
     2641 acttggagcc tctgatgaac ttatcgtaat tgcagaatcg tggaaggaag agcttcatac
     2701 aataagctca ttgcgccttg acacgcttgt cgcttcgtta gtgaattgtc cgcgtcaaaa
     2761 agcggcagcg ctcattcaga atgataaagt gaaagtgaac catgtcgtac gggatcagca
     2821 agcattcgag gtgaatgagt cggacatcct ttcaatcaga ggatcaggcc gttttaaaat
     2881 aatgtcgata gaaggaagga cccgaaaaga caaaatacgg ttgttgattg gtaggttaga
     2941 atgatttttc agacaaccgg ccgaaaaact gctttcttat ctgatgtggg catgtataat
     3001 agggaataga agatctactc gaaagggagg aacagaagat ggcattgtca ccactcgata
     3061 tacataacaa agaattcagc cgcggcttcc gcggatacga tgaagatgaa gtgaacgagt
     3121 ttctcgaaca gatcatgaag gattatgaaa acgtacttga tgagaataag acgttgaaaa
     3181 acagtttgaa ggaaacgaaa gaaaagattt cgcatttcaa tacaattgaa gagaccttgc
     3241 agaaatccat tttgatcgcc caagaagctg ctgaagatgt aagacggaac tcggtgcagg
     3301 aatcgaaact gattgtgaaa gaagcggaga agaacgcaga ccgtattgtg aatgaagcgc
     3361 tttcgcgtgc ccgcaagatt gcgatggata tcgaagactt gaagaaacaa tcgaaagtgt
     3421 tcaggaaccg tttccgcatg ttggttgaag cccagctcga tatgatcaaa acagatgatt
     3481 gggaaaagct gcttgaatat gaaccggata ctgagcatct tgaatctgca gcggacgtgg
     3541 aatgacgcgg aagcttgaca ttccattgcc ttcgcacata taataattac catataagta
     3601 ggaacaggcg ttgaaagaga cagtactttc cgatattcga gcatagcgag ccgggggcgg
     3661 tggaagcctg gcctgaaacg gaaacgaaaa tcactccaga gccggattga caaaatcaat
     3721 cccggatgca ccccgttatg gtgttctgag aggcagtatg atgatttatg ctgcaactag
     3781 ggtggtaacg cgagaatgat tcctcgtccc tttactaggg acgggttttt tttattttca
     3841 aaggaggaaa agatatggat tacaaagaca cattgctcat gccgaaaacg gaatttccga
     3901 tgcgcggaaa tttgccgaac aaagaacctg atatgcaagc aaaatgggaa gagatgaaca
     3961 tctacgagaa agtgcaaaaa cgcacggaag gtcgtccgtt cttcgtcctt cacgacggac
     4021 cgccgtatgc aaatggcgac ttgcatatgg gccacgccct taacaaagtg ctgaaagaca
     4081 tgattgttcg ccacaaatcg atgacaggct tccacgcacc ttacgtgccg ggctgggata
     4141 cacacggact tccgatcgag caagctcttg tcaataaagg cgtcaaccga aaagagcact
     4201 ccgttgcaga attccggaaa atgtgtgagg aatatgcata cagccaaatc gatagccaac
     4261 gtacacaatt caagcggatc ggtgtacgcg gcaactggga aaacccatat atcacattga
     4321 agcctgaatt cgaagcgaga caaattcaag tgttcggtga aatggccaag aaaggctata
     4381 tttacaaagg tctcaagcct gtctactggt cgccttcaag tgaatcggct cttgccgagg
     4441 cggaaatcga atacaaggat aaaaaatccc catccatcta tgttgctttt ccaatcaagg
     4501 atggtctagg cgttgttgac gaggatgtcc gaatcatcat ctggacgacg actccttgga
     4561 cgattcccgc taacctaggg atttcggtcc atcctgaatt cacgtatgca atcgtcaatg
     4621 tggacggaaa taaattcctc ctggcgaaag atctcgtcga gttcgttgcg aaagagatcg
     4681 gttgggaaac atatgagatt gtgaaagaac ttaaaggctc cgaactggat cgggtaattg
     4741 cgaaacaccc tctatatgat cgcgattctc tagtcatgct aggagagcac gtcactgcgg
     4801 aagccggtac gggatgcgtc catactgctc ctggtcacgg tgaagacgac ttttacgtat
     4861 cgaaacaata tggaatcgat gcattgtcgc cgatcaatga ccgaggcgtc atgacagaag
     4921 aggctccggg ctttgaaggt ttattctatg aagatgcgaa taaagaagtg acgaaaaagt
     4981 tggaggaaga aggcgcccta ttgaagcttt ccttcatcac tcactcctat ccgcatgatt
     5041 ggcgtacaaa gaagccggtc attttccgtg caactgctca atggttcgct tccattgaat
     5101 cattccgcga cgaattgctg gaagcaatcc gcaatacgaa gttcacaccg acttggggtg
     5161 aaacacggct gtacaatatg gtgcgagacc gtggagattg gtgtatttcc cgtcagcgtg
     5221 tatggggagt tccgatcccg gtgttttacg cggaaaacgg agaagcgatc cttaccgatg
     5281 aaacaattgc acatgttgc
//
LOCUS       DASULI010000201         4768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1849188,
            whole genome shotgun sequence.
ACCESSION   DASULI010000201 DASULI010000000
VERSION     DASULI010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849188"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFX34_04345"
     CDS             complement(<1..214)
                     /locus_tag="VFX34_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5564168.1"
                     /translation="MKATGIVVEYNPLHNGHVYHAQQARMATNADVIIAVMSGNFLQR
                     GEPAFVDKWTRTKMALASGVDIVFELP"
     gene            342..875
                     /locus_tag="VFX34_04350"
     CDS             342..875
                     /locus_tag="VFX34_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX5564169.1"
                     /translation="MKWSIHQLQKYRQGALPLDEAVNLDSVKKRNPEIRDIKPVHVTG
                     SCNIGSKKLTCHFHLEGVMTLPCSRTWEDVEYPFSVETDEQFSWDEATLATDDMIHPV
                     IGEIIDPTPVFEELVLLEVPLQVFSVNAEKMTSAEGKGWSYSTNEEHEERLRQEKENK
                     VDPRLAGLADFFNTDKE"
     gene            899..1072
                     /gene="rpmF"
                     /locus_tag="VFX34_04355"
     CDS             899..1072
                     /gene="rpmF"
                     /locus_tag="VFX34_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5564170.1"
                     /translation="MAVPKRRTSKTAKRLRRTHFKLQVPGMTTCENCGEMKLAHRVCK
                     SCGHYKGKDVVGE"
     gene            1147..1905
                     /locus_tag="VFX34_04360"
     CDS             1147..1905
                     /locus_tag="VFX34_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5564171.1"
                     /translation="MSYKIEMNNGIAKFIINRPEMRNAINHAVMDGLETFLSKVESDE
                     SIAFAVITGEGDRAFCSGGDLSEFHGFRTADEAYPMLSKMAGLLYRLSILPVPVIALV
                     NGAAVGGGCEIATACDYRLVSSKSKAGFIQGTLAITSGWGGASQLFIKMNNHDKAMQF
                     LSEARVHSAEQLHAFGWATYIYEGDAQAGLEQFISDMKRIHPSVHRAYKRLAILKWEQ
                     SGLGIHMLEEAAQCAKLWESDAHHEAVAKFLNKK"
     gene            2244..3605
                     /locus_tag="VFX34_04365"
     CDS             2244..3605
                     /locus_tag="VFX34_04365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5564172.1"
                     /translation="MNKILIANRGEIALRIMKTCKRLGIQTVAVHSEADEHMPFVEYA
                     DEAYVIGPGPVLQSYLKADDIINMALKVGADAIHPGYGLMSENAAFARKVRDAGLKFI
                     GPDAETIELMGDKIASRMKMKNAGVPVVPGTDEGVASLEDAIKSAEMIGYPIMLKASA
                     GGGGIGMVRCENEQALSQHFQSVKTRAKSYFGDDVVFLEKFVENARHIEVQIFGDDHG
                     NIVHLFERNCSVQRRNQKVIEESPSPSLNEDVGERLYEAAIAAGKAVDYRNAGTVEFI
                     LSENDEFYFLEMNTRLQVEHPVTEEVTGYDLVEWQIEVARGNELPVKEQRQIKSAGHA
                     IEFRIYAEDPATFMPSPGTIRTMKWNESANVRIDSGYEEGGSVTPFYDPMIAKVIVHA
                     SGRQEAIKEAEEFFASFTIEGVKTNIPLFQKFIGSDEFTNGNYNTNVLAEWLKTEKEE
                     VKK"
     gene            3602..3817
                     /locus_tag="VFX34_04370"
     CDS             3602..3817
                     /locus_tag="VFX34_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEX5564173.1"
                     /translation="MTQLKAAMAGTVFTVNVAEGEEVQAGQVIIVLESMKMEIPVETE
                     VGGKVTSINVQVGDFVNEEDVLAVIGE"
     gene            3881..>4768
                     /locus_tag="VFX34_04375"
     CDS             3881..>4768
                     /locus_tag="VFX34_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5564174.1"
                     /translation="MTEQKSYNEKLNERLEKVLSGGHPKYHEKLKEQNKLFVRDRLKL
                     LFDDGKYMEDGRFANCEAEDLPADGVVTAMGKVNGQTVCVMANDSTVKAGSWGSRTVE
                     KIIRIQEIAEKNRVPMLYLVDSAGARITDQLEMFPNRRGAGKIFHNQVRLSGFIPQIC
                     LLFGPSAAGGAYIPAFCDVVIMVDGNASMYLGSPRMAEKVIGEKVTLEEMGGARMHCS
                     VSGCGDVLAANEEEAISEARRYLSFFPANFTKKPLVKDAVAAKAGRSLEEIIPENQNA
                     PFDMYEAIDALIDEGSFFDI"
BASE COUNT         1429 a          955 c         1266 g         1118 t
ORIGIN      
        1 atggaagctc gaatacaatg tccacgccgg aagcgagcgc catcttcgta cgcgtccatt
       61 tatcgacaaa tgccggttcg cctctttgca ggaaattgcc gctcatgacc gcgatgatga
      121 catcggcgtt tgtagccatt ctagcttgct gtgcatggta gacatgtcca ttatgcaatg
      181 gattatattc aacgacgata cctgtagctt tcattatgtc caccccttcg atataatcta
      241 attgaattat acgttgtcgc gatttgggtg acaagaaaat atcttgacat ccctttttat
      301 ccattatata atcaacagtg ttgtcttgag gtgatataga cgtgaaatgg tccattcatc
      361 aactacagaa atacagacaa ggagcattgc cgcttgacga agcggtcaac cttgactccg
      421 tcaaaaaacg gaatccggaa atccgggaca ttaagcctgt ccacgtaacg ggtagctgca
      481 atatcggttc gaaaaaactg acttgccatt ttcatctcga aggtgtgatg acattgcctt
      541 gctcacgaac atgggaagat gtcgaatatc cattttcagt ggaaacggac gagcagttca
      601 gttgggatga agccactctt gcgacggatg acatgatcca cccggttatc ggcgaaatca
      661 tcgacccgac cccggtcttt gaagaactcg ttcttctaga agtgcctttg caggttttca
      721 gcgttaatgc cgaaaagatg acaagtgccg aggggaaagg ctggtcttac tcgaccaacg
      781 aagaacacga agagcggctc cgtcaagaaa aggaaaataa agttgatccg cgtttggcgg
      841 gtttggctga ttttttcaat acggacaagg aatagacgaa actaaggagg tgcctcaaat
      901 ggcagtacca aaaagaagaa cttcaaaaac agctaaaaga cttcgtcgta cgcatttcaa
      961 actacaagtt ccaggcatga caacttgtga aaattgcggc gaaatgaaac ttgcgcaccg
     1021 cgtttgcaaa tcttgcggtc attacaaagg aaaagacgta gtaggcgaat aagttcacct
     1081 gcttaaaaac caccgtgagg ccatggctcc ggtggttttt gttttatcca aaaaggaggg
     1141 gttaacatgt cctataaaat tgaaatgaat aatggaatcg ccaaattcat catcaatcgt
     1201 ccagaaatga gaaatgcgat caaccatgca gtcatggatg gacttgaaac gttcttatcc
     1261 aaagtcgaat cggatgaaag catcgcattt gcagtcatta caggcgaggg tgaccgtgca
     1321 ttttgttcag gcggcgacct atcggaattc cacggattcc ggactgcaga tgaagcgtac
     1381 ccgatgctga gcaagatggc tggattgttg tacagattat ccattttacc tgtgccagtc
     1441 attgcacttg tcaacggagc ggcagtcggt gggggatgtg agattgccac tgcgtgcgat
     1501 tacaggctcg tatcttcaaa atcgaaggcg ggattcatcc aagggacact tgccataaca
     1561 agcggctggg gcggcgcatc gcaattattt ataaaaatga acaatcatga taaagcgatg
     1621 cagttcttat cggaagcgag agtgcattcg gcggaacagc tacacgcatt tggttgggct
     1681 acgtacattt atgaagggga tgcacaagct ggcttggagc aattcatttc agatatgaag
     1741 agaatacacc catccgtaca tcgggcttat aaaaggttgg cgatcttaaa atgggagcaa
     1801 agcggactgg ggatccacat gcttgaagaa gcggcacaat gcgcaaaact atgggaaagc
     1861 gatgcccacc acgaagctgt agcaaaattc cttaataaga aatgaaaaaa tagtgcagct
     1921 atcaggattg gagcgaaagc cgtaacgaaa gaacggcttt tgcgagtata agcgtagcgt
     1981 tacgagcaat gttttttgcg accctacggg agcaggaagt agaagtagct gccttaattt
     2041 ctgcaaaact gcagaaatac ggcaaatcga actcttcgtg gttcgattgg aaagcatccg
     2101 cctgaagcgg aaatccatta cagaggtcta tccaaggtca gattttcaaa ggaattcaga
     2161 caaatagttg aaaacggttt cattcatcgt ataaactata agtggggaga agttcaaaca
     2221 actcagctta gggggattac aatatgaata agatacttat tgcgaatcgt ggggaaatcg
     2281 cattgagaat catgaagacc tgcaaacggc tcggcatcca aacagtcgcc gtccactcgg
     2341 aggccgacga acatatgcca ttcgtggaat atgcagacga ggcttatgta atcggacctg
     2401 gcccagtact gcaatcctac ttgaaagcgg atgacattat taacatggca ttaaaagtgg
     2461 gggcggacgc aattcatccc ggctatgggc ttatgtccga aaatgccgct ttcgctcgaa
     2521 aagtgaggga tgcgggtttg aaattcatcg gcccggacgc agaaacaatc gaattgatgg
     2581 gtgataaaat cgcttcaaga atgaagatga aaaatgcagg ggtgccggtt gttccaggaa
     2641 ctgatgaagg agtcgcctct ttggaggatg cgataaaatc cgcagaaatg atcggatatc
     2701 cgattatgct gaaagcgagt gccggcgggg gcggcatcgg aatggtccga tgtgaaaatg
     2761 agcaagcgct cagtcaacat tttcaatccg tcaaaacccg ggcaaaatcc tatttcgggg
     2821 acgatgtggt ttttcttgag aaattcgtag aaaatgcgcg ccatatcgaa gtgcagattt
     2881 tcggggacga ccatgggaat atcgtccatc tattcgaacg gaattgctca gtacaaaggc
     2941 ggaatcagaa agtgatcgaa gaatcgccgt ctccttcctt gaacgaagac gtcggagagc
     3001 ggctatacga agctgcgatt gctgctggaa aagcggttga ctatcggaac gccggaacag
     3061 tggaattcat cttatcggaa aacgatgaat tttactttct agaaatgaac acccggctac
     3121 aagtggagca tccagtcact gaggaagtga ccggttatga tcttgtggaa tggcagattg
     3181 aagttgcacg gggcaatgaa ttgccggtca aagaacaacg gcaaattaaa agcgcgggcc
     3241 atgcaattga attccgcatt tatgcagaag atccggcaac cttcatgcca tcaccgggga
     3301 caatccgaac aatgaaatgg aatgagtcgg ctaatgtgcg cattgattcg ggatatgaag
     3361 agggcggaag cgtaacgcct ttctatgatc cgatgattgc aaaagtgatc gtccatgcga
     3421 gcgggcgtca agaagcgata aaggaagcgg aagaattctt cgcttcgttt acgattgaag
     3481 gagtaaaaac gaatattcca ctctttcaaa agttcatagg ttcagatgaa tttacgaatg
     3541 gaaattataa tacgaatgtg ttagcggaat ggctgaaaac agaaaaggag gaagtgaaga
     3601 aatgacacaa ttaaaagctg ccatggcagg tacagtattt acagtgaatg ttgctgaagg
     3661 cgaggaagta caggctggcc aagtgattat cgtgcttgag tcaatgaaaa tggagattcc
     3721 cgtagaaacg gaagtgggag gcaaagtgac atccatcaat gtgcaagtcg gcgatttcgt
     3781 gaatgaagag gatgtacttg cagttatcgg ggaataaggg cagctggcag atgtgccctt
     3841 aagggtgaat gtgatcggaa ggggtaaaac gatgacgaat atgactgaac agaaaagcta
     3901 taatgaaaaa ttgaatgaga gacttgaaaa agtgttgtcg ggtggtcatc cgaaatatca
     3961 tgaaaaattg aaggagcaga ataagctgtt tgtacgagat cgccttaagc tccttttcga
     4021 tgacggaaaa tatatggagg atggccgctt cgcaaattgt gaagcagaag atctgcctgc
     4081 agacggcgta gtaacagcga tggggaaagt gaatgggcaa accgtctgcg tcatggcgaa
     4141 tgattcgaca gtaaaggctg gttcttgggg ctcgaggacg gtcgaaaaaa ttatccgtat
     4201 ccaggagatt gcggaaaaga atagagtgcc gatgttgtac cttgtcgatt ctgcaggagc
     4261 acggattaca gatcaacttg aaatgttccc gaatcgccgc ggtgcgggaa agatcttcca
     4321 taaccaggtg aggctttcgg gtttcatacc gcaaatctgc ctattgtttg ggccttcggc
     4381 tgcaggcggg gcatacatcc cggccttctg cgatgtcgtc atcatggtgg acggcaacgc
     4441 atccatgtat ttagggtccc ctaggatggc ggaaaaggtg atcggcgaaa aagtgacatt
     4501 ggaggagatg ggcggcgcac ggatgcattg ttccgtcagt ggttgcggcg atgtcctcgc
     4561 agcaaacgaa gaggaagcaa ttagcgaagc acgtcgctat ctaagctttt tccctgcaaa
     4621 cttcactaaa aagcctttag tcaaagacgc agtagccgca aaggcgggaa ggtctctgga
     4681 agagatcatc ccggaaaacc agaatgcgcc gttcgatatg tatgaagcga tcgatgcatt
     4741 gattgatgaa ggcagtttct ttgatatt
//
LOCUS       DASULI010000202         3553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_402482, whole
            genome shotgun sequence.
ACCESSION   DASULI010000202 DASULI010000000
VERSION     DASULI010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402482"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..2160)
                     /gene="scpA"
                     /locus_tag="VFX34_04380"
     CDS             complement(7..2160)
                     /gene="scpA"
                     /locus_tag="VFX34_04380"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEX5564175.1"
                     /translation="MNKPDFNKVDISKLNKAVNPSGNETSFLTNEGISIKSSYTNKDL
                     EGISHLNDMPGIAPNTRGPYPTMYVARPWTVRQYAGFSTAEESNAFYRRNLAMGQKGL
                     SVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDGIPLDQMSVSMTMNGAV
                     LPIMAFYIVAAEEQGVSPEQLAGTIQNDILKEYMVRNTYIFPPEMSMRIIADIFEYTS
                     KNMPKFNSISISGYHMQEAGATADIELAYTLADGLEYVRTGLKAGIDIDSFAPRLSFF
                     WAIGMNYFMEIAKMRAARKIWAQMMQSFHPSNPKSLALRTHSQTSGWSLTEQDPFNNV
                     TRTLVEANAAAMGHTQSLHTNALDEAIALPTDFSARIARNTQLFLQEETNMTKTIDPW
                     GGSYYVERLTADLMEKAWELIEEIEDLGGMAKAIETGLPKMKIEEAAAKRQAKIDSRT
                     ETIVGVNKYRLEKEEPIDILEIDNTVVRQKQIDRINQMKGMRDEAKVTEALETLTEAA
                     RNGNGNLLALSVNAARARATIGEISDAIEKVSGRHKAVIRSVSGVYSSNFSNAEEIEE
                     VKAMADEFLDNEGRRPRILIAKMGQDGHDRGAKVIASSFADLGFDVDIGPLFQTPEET
                     ALQAAENDVHVIGVSSLAAGHKTLVPALRDELSKIGRADILIVVGGVIPAQDYDFLRE
                     NGATAIFGPGTVIPVAAQKVIEDIYRALGYEEVTE"
     gene            complement(2157..>3553)
                     /locus_tag="VFX34_04385"
     CDS             complement(2157..>3553)
                     /locus_tag="VFX34_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5564176.1"
                     /translation="KGNEAIVYDGQQPFDWNDDQLQSLSSIIEKFPVFLFNVRNDDPI
                     LRAFDFVHHEVRESVTGIIQVADWTVPVGYNKLRTAGADLWKIHHGGADAVTELAIAL
                     SEASRIASSFESFQAFADNFFVRFPIDTHFFMEIAKLRAFRVLWQAFAQAYAIDEPPF
                     IPVFAVTSLRSFSKLDPYVNLLRAGNETFAAILGGADVIQAHPHNMLTGPNASSIRYS
                     RNMQLVLKEEAHVEKVLDPAGGSYFIEQLTEELVEKSWKLFLEIESKGGIDKFLESGR
                     LQENLLQLQYETATGKRALIGTNVYAELTTTNFDDWSGMNSTARLAEPFEKLRQSFSE
                     KQPKTVILTFGKLKDFKPRADFVTSFLASGGIKSRWSPAMESIEQAVEWLDNEKPDYA
                     IICGNDEIVSAVMESLLEKLPADIMLDVAGKIDEKTFHKWEQLGLDGMIFNGQNKIEK
                     LQGVLTSWQGGTRK"
BASE COUNT          857 a          910 c          755 g         1031 t
ORIGIN      
        1 tcttgttcac tccgtcactt cctcataacc gagtgctctg taaatatcct cgatcacttt
       61 ctgtgcagcg acaggtatga ctgttcctgg gccaaaaatg gctgtcgcgc cgttttccct
      121 gaggaaatca taatcctgcg caggaatcac tccgcctaca acgattaaaa tgtctgccct
      181 tccaattttt gaaagctcat cgcgtaatgc cggaacaagc gttttatgcc ccgcggcaag
      241 tgaactgacg ccgattacat ggacatcatt ttccgccgcc tgcaaagctg tctcttcagg
      301 agtctggaat aaaggaccga tatccacgtc aaatcctaaa tctgcaaagg aagatgcaat
      361 cactttcgct ccgcggtcat ggccgtcctg ccccattttc gcgataagga tgcgcgggcg
      421 tctgccttca ttatccaaaa actcgtctgc catcgcctta acttcttcta tctcttcggc
      481 attggagaaa ttggaactgt aaacgccgct tacagaccgg atcaccgctt tatgccttcc
      541 cgatactttt tcaatcgcgt cggaaatttc gccgattgtc gcccttgccc tggcagcatt
      601 cacggataac gctaggagat ttccatttcc gtttcgcgca gcctctgtca aagtttccaa
      661 agcttcggtg acctttgctt catctcgcat gcctttcatt tgattgatgc gatcgatctg
      721 tttttggcgg acaactgtat tgtcgatttc caatatatcg ataggttctt ccttttccag
      781 ccgatattta ttgactccga caatcgtttc agtgcgtgag tcgattttcg cttgacgttt
      841 ggcagcggct tcctcgattt tcatcttcgg caaaccggtt tcgattgcct ttgccatccc
      901 gcctaagtct tcgatttcct cgatcagttc ccatgctttt tccataagat cggcggtcaa
      961 cctttcaaca tagtaggaac cgccccaagg gtcaatcgtc ttcgtcatat tcgtttcctc
     1021 ttgcaggaac aattgcgtat tccgtgcaat cctagccgag aaatccgtcg gcaacgcaat
     1081 cgcttcatcg agtgcatttg tatgcaaaga ttgtgtatgg cccatggcgg ctgcattcgc
     1141 ttcaaccaat gtgcgcgtca cattgttgaa aggatcctgc tccgtcaaac tccatccaga
     1201 cgtctgggag tgcgtccgca atgcgaggga tttagggttg gacggatgga aggactgcat
     1261 catttgtgcc caaatttttc gcgccgcccg catttttgca atttccatga aataattcat
     1321 gccgatcgcc caaaagaatg aaagacgagg cgcgaatgaa tcgatgtcga tgcctgcctt
     1381 caaaccggtg cgaacgtact caagcccatc cgccaatgtg tacgcaagct cgatatccgc
     1441 cgttgccccc gcttcctgca tatggtaacc cgaaattgaa atcgaattga atttcggcat
     1501 gtttttggat gtgtattcaa aaatgtcggc aatgatgcgc atcgacattt ccggcggaaa
     1561 aatgtatgtg ttcctcacca tatattcttt taaaatatcg ttttgaatcg tgcccgcgag
     1621 ctgctcaggg gacacaccct gttcctccgc cgcaacgatg taaaaagcca tgatcggcag
     1681 aacagcgcca ttcatcgtca tcgatacgga catctggtca agcggtatgc cgtcgaaaag
     1741 gattttcatg tcttcaacgg aatcgattgc aaccccggcc ttcccgacat cacctgtcac
     1801 acggggatga tcggaatcat agccacggtg tgtcgccaag tcaaacgcaa ccgaaaggcc
     1861 tttctgcccc atcgccaagt ttcttctata aaatgcatta ctctcttctg ctgttgaaaa
     1921 tccggcatat tgacggacag tccaaggtcg agctacgtac atcgtcgggt atggacctct
     1981 cgtattcggg gcgatgcccg gcatgtcatt caaatgtgat atgccttcca gatctttatt
     2041 tgtgtaacta gatttgattg aaataccttc attcgtaaga aatgacgttt cgtttccgga
     2101 aggatttaca gctttgttca gtttgcttat gtcaacttta ttgaagtcag gtttattcat
     2161 ttccgggtcc ctccttgcca cgaagtcaat acgccctgca atttctctat tttgttctgg
     2221 ccgttgaaaa tcatcccgtc aaggccgagt tgctcccatt tgtgaaaagt cttttcgtcg
     2281 attttccctg ccacatccag cattatgtcc gctggcagct tttcaaggag actctccatg
     2341 acagccgata caatttcatc attgccgcaa ataattgcgt aatcgggttt ttcattatcc
     2401 aaccattcga ccgcctgttc aatgctttcc atagcagggc tccatctgct tttgattccg
     2461 ccggaagcta agaaactagt gacgaaatcc gctcgcggct taaagtcctt tagtttgccg
     2521 aatgtaagaa ttaccgtttt aggttgtttt tcactgaatg attgacggag tttttcaaaa
     2581 ggctccgcca gacgggctgt cgaattcatg ccactccaat catcgaagtt tgtcgttgta
     2641 agctcagcgt aaacattcgt gccgattagc gcccgtttac cagttgcagt ctcatattga
     2701 agttgtaata agttttcctg taatcggccg ctttccagga atttatcgat tccacccttg
     2761 ctctcaattt ccaaaaacaa cttccaagat ttttcgacga gttcttcagt cagttgctca
     2821 ataaaataag agcctcctgc cggatcaaga actttttcca catgcgcttc ttcttttaaa
     2881 acaagttgca tattacggct gtaccttatg gaacttgcgt taggaccggt aagcatattg
     2941 tgtggatgcg cttgtataac atcggctccc cctaaaatag cggcaaatgt ttcattccct
     3001 gcacgaagca ggttgacgta tggatccagc tttgaaaaag aacgcaaaga tgtaacggca
     3061 aacactggaa taaacggtgg ctcgtcgata gcataggctt gtgcgaacgc ttgccataat
     3121 actcggaaag ctcgtagctt agcgatttcc atgaaaaaat gagtgtctat tggaaagcgc
     3181 acgaaaaagt tgtctgcaaa tgcctggaag ctttcgaagc ttgaagcaat ccgtgaagct
     3241 tcagacaatg caatcgctag ttcagtgacc gcatctgcac ctccatggtg tattttccat
     3301 aagtccgctc ctgcagtccg gagtttgtta tacccaactg gaacagtcca atctgccacc
     3361 tgtatgattc ctgttactga ctcccgaact tcatgatgca caaaatcgaa tgcccttaaa
     3421 atcgggtcgt cgtttcgcac attgaaaagg aatacaggga atttttcgat gattgaagat
     3481 aaactttgca gctgatcatc attccagtca aatggttgtt gaccatcata gacgattgcc
     3541 tcgttccctt ttt
//
LOCUS       DASULI010000203         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_864053, whole
            genome shotgun sequence.
ACCESSION   DASULI010000203 DASULI010000000
VERSION     DASULI010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864053"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFX34_04390"
     CDS             <1..189
                     /locus_tag="VFX34_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564177.1"
                     /translation="TISMLGNAKQLFVYVDSRNRAGRAFYEKQGFELLEVFEESFEGY
                     PVETAQYVYNIHKPVLAY"
     gene            complement(232..702)
                     /locus_tag="VFX34_04395"
     CDS             complement(232..702)
                     /locus_tag="VFX34_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEX5564178.1"
                     /translation="MNRTAEKVLSIIALVLNVIGAIVAFIGLAVFNLLKNDPSFKQEF
                     EMEMMADPALTSADIEAMYAVMNVLGGFIWLIIIGIIISIVLNIIGIVNIWKNKNAKL
                     AGIMFIIAGALGGLLSLQSILLYVAAVLCFTKKPPLQDNLQYADQYDGDGMRPL"
     gene            complement(1058..1339)
                     /locus_tag="VFX34_04400"
     CDS             complement(1058..1339)
                     /locus_tag="VFX34_04400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564179.1"
                     /translation="MEFEFVELGGDAFAFRHEKDGKMLAEITWTLLADVMVMDHTYVS
                     QELRGGGVAKQLLDRAAEYAREKGYKMEAVCSYVVTAFNRYKEYDDVKA"
     gene            complement(1386..2249)
                     /locus_tag="VFX34_04405"
     CDS             complement(1386..2249)
                     /locus_tag="VFX34_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEX5564180.1"
                     /translation="MAFLLIIGGLLPTNAKADEAIDEKLGVPIVVMGANLTDAEKQSV
                     KKSLKVEENAEVEEITVDGNDLVKYIKDGNSQARMYSSAKITRQDKGKGLVINIVTPD
                     NITQVTNEMYATAMMTAGIEDAIVEVAAPKPVTGHSALVGIYKAYEVSGETLDTERTD
                     VANDELNLATKFAKDSGIDQDKVAELLTEIKKAIADNKPATKEEVEKIVNDQLAKLNI
                     ELSEADRQLLIDLMDRIRKLDIDFGKLSEQLSDMATTIKEKFGEIDPGFWDKVKQFFA
                     DLFESIKSWFK"
     gene            complement(2333..>2514)
                     /locus_tag="VFX34_04410"
     CDS             complement(2333..>2514)
                     /locus_tag="VFX34_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX5564181.1"
                     /translation="ETLYRYLTDVGGKMPFSDKSTPDEISEMFGMSKGAFKRALGKLM
                     KDGKVSQSEGWTTLK"
BASE COUNT          624 a          630 c          496 g          764 t
ORIGIN      
        1 acaatcagca tgctcggcaa tgctaaacag ctattcgtgt acgtagatag ccggaataga
       61 gccggacgag cattttacga gaagcaagga tttgaattgc tggaggtatt cgaggagtct
      121 ttcgaaggat accctgttga aacagcccag tatgtctaca atattcataa accggtacta
      181 gcctattaag gcaaaagacg cttccccgtc aagggcaagc gtctttttag tttacagcgg
      241 tctcattccg tcgccgtcat attgatccgc atattgaaga ttgtcctgca atggcggctt
      301 tttcgtaaag caaaggaccg ctgcgacata taacaggatg gattgcaaag aaagaagccc
      361 tcctaaagca cctgcgatga tgaacataat ccctgcaagt ttggcattct tgtttttcca
      421 gatgttgacg atgccaatga tattcaacac gatgctgata atgattccga tgatgatcag
      481 ccagatgaag ccgcccaaaa cattcatgac cgcatacatc gcttcaatgt cagctgacgt
      541 caacgccgga tcggccatca tttccatttc aaattcctgt ttgaacgacg gatcgttttt
      601 caataaatta aagactgcta atcctataaa tgcaacgata gcgccgatta cattcaggac
      661 caatgcgata atggataata ccttttctgc tgtacgattc aagtgaagca ccctttctct
      721 ctacattttt tctatcataa caagtatacg agagattaaa caataagttt cgtttacagt
      781 ccattttttc acaaggtaag ttttgggttt gtttattgtg ggtactatac tcctatgaag
      841 gtaaaacatc atgaagcgag aggagagcat gaaatatgag ggacaaaata atgttcaact
      901 cgacggacat gctttatacc gtcgcaggcg catctgcact gacgagcatc ttatatttcg
      961 tcacaattgc atggggactt taattcatat ttggcaaaat taaaagacaa tcctttattc
     1021 attgaaggat tgcctttttt tgcgtttttg tttgtagtta tgcttttaca tcatcgtatt
     1081 ccttgtatcg attaaacgca gtgactacgt aggagcagac ggcttccatc ttatatcctt
     1141 tttcgcgggc gtactccgca gcacggtcga gcagttgttt cgctacgcct ccgccgcgca
     1201 attcttgtga cacatatgtg tggtccatga ccatgacatc tgcgagaagc gtccatgtga
     1261 tttcagctag cattttaccg tctttttcat gacgaaacgc gaatgcgtca ccgccaagct
     1321 cgacaaattc aaattccatt gtgtctcact ccttttccaa tattctatca tattgttcag
     1381 ggttttcact tgaaccatga cttgatcgat tcaaaaagat ccgcaaagaa ctgtttcact
     1441 ttatcccaga agccgggatc gatttcgccg aacttctctt tgattgtcgt tgccatatcg
     1501 gataattgct cggacaattt gccgaagtcg atatccaatt tccggatcct gtccatgaga
     1561 tcgatcaaca attgccgatc ggcctcgctc agctcgatat tcagcttcgc caattggtca
     1621 ttgacaattt tttcgacttc ttccttcgtt gcgggcttgt tatcggcaat cgccttcttg
     1681 atttccgtta ggagctccgc cactttgtcc tggtcgatgc cggaatcctt cgcaaacttc
     1741 gtcgccaaat tcaattcatc atttgcaaca tccgtccgct cggtatcaag cgtttctccg
     1801 gacacttcgt atgccttgta gatccccact aatgcggaat gtcccgtcac cggcttcggc
     1861 gccgccactt cgacgatggc atcctcgatt ccggcagtca tcatcgccgt tgcatacatt
     1921 tcgttcgtca cttgtgtaat attgtcaggt gtgacgatgt tgatgacgag acctttccct
     1981 ttatcctgcc ttgtaatttt ggcggatgaa tacattctcg cctggctgtt tccgtctttg
     2041 atatatttca caagatcatt tccatcgacc gtaatctctt cgacttcggc attctcttcg
     2101 actttcaagc tctttttaac gctctgcttt tccgcgtctg tcaagtttgc ccccattaca
     2161 acaataggca cacccagttt ttcatcaatt gcctcatcgg ctttcgcatt tgtcggcagc
     2221 aagcccccga tgatcagcaa aaatgccatc gccaccatca cgattctctt cataagcttt
     2281 cccctcctgt actatgaagt agtacgacgt gcaatcaaaa aagttccaac tctcatttca
     2341 aggtcgtcca accttcgctt tgtgatactt tgccatcctt catcaatttg ccaagcgcac
     2401 gtttgaatgc acccttgctc ataccgaaca tttcgctgat ttcatcgggg gtcgatttat
     2461 ccgagaacgg cattttgccg ccaacatccg tcaagtaccg gtacaacgtt tccg
//
LOCUS       DASULI010000204         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1144671,
            whole genome shotgun sequence.
ACCESSION   DASULI010000204 DASULI010000000
VERSION     DASULI010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144671"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFX34_04415"
     CDS             complement(<1..554)
                     /locus_tag="VFX34_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX5564182.1"
                     /translation="MSTFIRGTAILMIAVFMSKLLGFVYRIQFMRVAGEEAVGIYMTA
                     YPAFIFFLSLVQLGTPIAVAKVIAELKVKGETAKLSAVMRTATFITIFTGTVFIPLSI
                     LFVPYLAETLLGNAASSTTLYVGIAIIPIASVCGLIRGYFQGIVRIEETAWSQIIEQL
                     FRIVLITWLLPIMLIPDNNPMNAA"
     gene            651..962
                     /locus_tag="VFX34_04420"
     CDS             651..962
                     /locus_tag="VFX34_04420"
                     /inference="COORDINATES: protein motif:HMM:NF025169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-transcriptional regulator"
                     /protein_id="HEX5564183.1"
                     /translation="MTIQFEQIFKHALPAIQSKRDEFRLYGYPTVTNEEIWKYCIRKK
                     WRKKNVSTMRIHEIVNGVLALTPAEFMTYTQIEEQRDSNWFSDLNSDELQLLLSPKSK
                     S"
     gene            1093..3360
                     /gene="secDF"
                     /locus_tag="VFX34_04425"
     CDS             1093..3360
                     /gene="secDF"
                     /locus_tag="VFX34_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HEX5564184.1"
                     /translation="MKKRSRIVAFLLLLVMFGSLIGVTANPIIKDVRLGLDLQGGFEV
                     LYEVQPLKEGQKITEDIVTDTAGALRNRVDVLGVSEPVIQVESNNRIRVQLAGVEDQE
                     SARELLSTQANLTFRDADDNLMLDGNDLKEGKAKANFGQNGNPVVTLEMKDPDKFGEV
                     TAEIKAKAPENVMVIWMDFEEGVDSFKEERMKEKPKFISAPYVSHVINSSNVEISGSF
                     TVEETKKLAGILNAGALPVHLEEIYSTSVGAQFGAQALDKTILAGIVGIALIFLFMLI
                     YYRVPGFVAVITLSVYIYLILTVFTSINGVLTLPGIAALMLGVGMAVDANILTYERIR
                     EELRVGKSVKTAFSVGAKSAFTAILDANITTLLAAIVLFIFGTSSVKGFATMLIISIL
                     VSFLTAVWGSRLLLGLLVNSGMLDGKTGLFGISKRRIHAPEENVDTLDLTTKFDRFDF
                     VHSRKKFYVLSMALIIAGIIVLGIFKLNLGIDFAGGTRVEILSDTPLTAEEVSGTLEE
                     IGHPTTDIVISGDKHNIGVARYKEEFKQETVNKFKKDLESHYGADPNISTVSDTVGRE
                     LAGNALKALLVAALGIVIYVAFRFEWRMGVSSIIGLIHDAFFMVAVFSITRLEVDITF
                     IAAVLTIVGYSINDTIVTFDRIRENLHKVGKINNANELADIVNKSLRQTLGRSVNTVL
                     TVVFVVVALIALGAEAIRPFSIALLIGLLAGTYSSIFISAQIWFDLKKKELEKTGTLK
                     VDKEKKQWGSDEPVV"
BASE COUNT          994 a          703 c          806 g          947 t
ORIGIN      
        1 taagctgcat tcatcgggtt gttatcaggg atgagcataa ttggcaacaa ccacgtgatt
       61 agcacgatcc ggaatagttg ttcgatgatc tgggaccaag ccgtttcttc tattcttaca
      121 attccttgaa aatatccgcg gatcaaaccg catacagaag cgatcgggat gattgcaatc
      181 cctacatata aggtagtgga agaagccgca ttgcccaaca aagtttcggc taaatacggt
      241 acgaataaaa tggatagcgg gatgaatact gtacctgtaa aaatcgtaat gaacgtcgcc
      301 gtcctcatga ctgcggaaag ttttgcggtt tcgcctttca ctttcaactc cgcaatcact
      361 ttggcaaccg caatcggtgt tccaagctgc acaagcgaca gaaagaatat gaaagcggga
      421 tatgcggtca tatagattcc gaccgcttcc tcgcccgcca cacgcatgaa ttggatacga
      481 tagacgaatc ctaatagctt ggacatgaaa acagcaatca tcagtatggc agttccacgg
      541 atgaaagtgg acaataaaaa caactccttc tcaattccgg tcatttcgta tacactaaat
      601 ctatgcaaag gtttgtctat ttacgactga acgggaaagg gatggagaag gtgacgatac
      661 aattcgagca gatattcaaa catgcattgc ctgctattca gagcaagaga gatgaatttc
      721 gcctttatgg ctatccgact gtgacaaatg aagagatatg gaaatattgc atacgaaaaa
      781 aatggcggaa gaaaaatgtt tcgacgatgc ggatccatga aatcgtgaat ggcgtgttgg
      841 cattgacgcc tgcggaattc atgacgtata cacaaatcga ggaacaaagg gactcgaact
      901 ggttctcgga cctgaacagc gatgaactcc agctgctttt atcaccgaaa agcaagtcct
      961 gacacgagtt ttgtgaatgt ccaactattt gacagatgaa aatctctgtt tcataattgg
     1021 attgttgtat tttcttaaaa actgtacata gcaatcttct caccgctcca cgtacagagg
     1081 gggaactaac ttatgaaaaa aagaagccgg attgtagcgt ttctattatt gcttgtcatg
     1141 tttggatctt tgataggcgt tacggcaaat ccgattataa aagatgttcg gctcggtctt
     1201 gacctacaag ggggcttcga agtcctttac gaggtgcagc ctcttaaaga aggtcaaaaa
     1261 attacggaag atatcgtcac cgacacagca ggtgctttac gtaaccgggt tgacgtgctt
     1321 ggtgtaagtg aaccagtcat ccaggtcgag tccaataaca ggatccgtgt gcaattggcc
     1381 ggtgtggaag accaggagtc agcacgtgaa ctattatcca cacaggcaaa cctgacattc
     1441 agggatgcag acgacaatct gatgttggat ggaaatgatt tgaaagaagg aaaggcgaaa
     1501 gccaacttcg gtcaaaatgg taatccagtt gttacactcg agatgaagga tccagataag
     1561 tttggggaag taacagctga aataaaagca aaagcgcccg aaaacgtaat ggtcatctgg
     1621 atggattttg aagaaggtgt cgattcattc aaagaggaaa gaatgaagga aaaaccaaaa
     1681 ttcatttctg cgccttatgt aagtcatgtt atcaactcat ccaatgttga aatttcagga
     1741 agcttcacag tggaagagac caagaaattg gcgggcatct tgaatgccgg agcactccca
     1801 gtccaccttg aagaaatata ctctacttct gttggtgctc aattcggtgc acaggcattg
     1861 gataaaacaa ttctggctgg gattgtcggt atagctctaa ttttcctatt catgttgatt
     1921 tattaccgcg ttcctggatt tgtggctgta attacacttt ctgtctatat ttatcttatc
     1981 cttactgtct tcacaagcat taacggagtt cttacacttc cgggaatcgc agccctaatg
     2041 ctcggggttg gtatggcagt cgatgcgaat atcctgacgt acgagaggat tagagaagaa
     2101 cttcgtgtag ggaaatcagt gaagacggca ttctcggtcg gtgcgaaatc agcattcacc
     2161 gcaatcttgg atgccaatat aacaactttg ctcgcagcta tcgtcctttt cattttcggg
     2221 acaagctccg tcaaaggctt tgcaacaatg ttgatcataa gcatcctcgt cagcttcttg
     2281 acagctgttt ggggttcacg tctactgttg ggtcttctcg tcaatagcgg gatgcttgat
     2341 ggcaagactg gtctattcgg aatctcgaaa cgccggatcc atgcgccaga agaaaatgtg
     2401 gacacgcttg atcttacaac gaaattcgat cggtttgact ttgttcattc ccgtaagaaa
     2461 ttctatgtcc tttccatggc attgattatt gccggtataa ttgttctcgg gattttcaaa
     2521 ttgaatcttg ggatcgactt tgcgggcggt acgcgtgttg aaattctatc ggacactcct
     2581 ctaacggctg aggaagtctc tggaacgctt gaagaaattg gtcacccgac tactgacatt
     2641 gtcatttccg gtgataaaca caatattggc gttgctcgtt ataaagaaga atttaagcaa
     2701 gaaactgtca ataaattcaa aaaagatttg gaatcccact acggtgcgga cccgaatatt
     2761 tcaacagttt ccgatacagt tggtagagaa ctcgcaggaa atgcgttgaa agcattgcta
     2821 gtcgccgcac tcggaatcgt tatctatgtt gcattccgtt tcgaatggcg tatgggtgtt
     2881 tcgtccatta ttggtctgat ccatgatgca ttcttcatgg tagcggtctt cagtataacg
     2941 aggcttgaag tggatatcac attcattgca gccgtattga caattgtcgg gtattccatc
     3001 aatgatacaa tcgttacgtt cgaccggatc agggaaaacc tgcataaagt cggcaaaatc
     3061 aataatgcga atgaattggc ggacatcgtc aataaatcac ttcgccaaac gttgggccgt
     3121 tcagtgaaca ccgtcttgac agtcgtcttt gtagttgttg cactgattgc tcttggcgcg
     3181 gaagcgattc gtccattctc gatcgcattg ctgatcggat tgcttgccgg tacgtattct
     3241 tccatcttca tctcagctca aatctggttt gacctgaaga agaaagagct tgaaaaaacg
     3301 ggaacgttga aagtggataa agagaaaaaa caatggggtt ctgacgagcc ggtagtctga
     3361 taacacaagc ttgactggcg tttcatacct ctatggaaca gggtatacct agtgtatatc
     3421 ctgttttttg tttttatatt tagaaaattt
//
LOCUS       DASULI010000205         4094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_625399, whole
            genome shotgun sequence.
ACCESSION   DASULI010000205 DASULI010000000
VERSION     DASULI010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625399"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /gene="dnaX"
                     /locus_tag="VFX34_04430"
     CDS             <1..1598
                     /gene="dnaX"
                     /locus_tag="VFX34_04430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5564185.1"
                     /translation="TSAAKIFAKTINCEQAPVAEPCNECPTCLSITEGSNTDVIEFDA
                     ASNSRVEEMRDIIEKVRFAPSNARFKVYIIDEVHMLSNSAFNALLKTLEEPPAHVVFI
                     LATTEPYKLPLTIISRCQRFDFKPHTISDIIARLRKVLDDSGIKSDEGSLKIIAQAAS
                     GGMRDALSILEQVVSFSGDELTVEDALLVTGAIGEDIFYQLAEALLGKDAGTALSLLD
                     QLIAEGKDVSRLAEDMITFFRDLLLLRTAPALKDLLELVTGDERFEEMAKQFNPDVLY
                     SYIDILSKTQQEMRFSNYAKVYLESALLKMVNAAQDLSTGTAAQSGDLVEKVQNLERM
                     IAELQRKLAEGVPVAAVPADTAQPRKPASRPTSSYKIPVGKINEVLKEATKQDIMTIR
                     EQWANMMQTMQKSHAALLEETEPVAASERAFVLKFKYEIHCGMASDNPSLQSSLTQAL
                     LSRTGKAYDVLFVPEEGWLKVREDFIRNSGLAKQSDQQAEDEQTADEMPSFMEEAEAS
                     TSDPLVSEAEKLFGKEFVEVYDD"
     gene            1620..1940
                     /locus_tag="VFX34_04435"
     CDS             1620..1940
                     /locus_tag="VFX34_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5564186.1"
                     /translation="MRGMGNMQGMMKQMQKMQKKMAEAQEKLGEERLEGTAGGGMVKV
                     VVSGHKEVVDVVIDPSVVDPEDVEMLQDLLIVATNDAMSKAEELTNSTMGQFTKGLNL
                     PGMF"
     gene            1956..2552
                     /gene="recR"
                     /locus_tag="VFX34_04440"
     CDS             1956..2552
                     /gene="recR"
                     /locus_tag="VFX34_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5564187.1"
                     /translation="MHYPEPISKLIDSFMRLPGIGPKTAARLAFFVLSMKEDNVLEFA
                     KALVDAKRNLQYCSNCGHITDVDPCQICQDKSRDGSMICVVQDPKDVIAMEKMRDYNG
                     YYHVLHGAISPMDGIGPEDINVPSLLTRLQNEEVQELILATNPTIEGEATAMYISRLV
                     KPSGIKTTRIAHGLPVGGDLEYADEVTLSKALEGRREL"
     gene            2567..2788
                     /locus_tag="VFX34_04445"
     CDS             2567..2788
                     /locus_tag="VFX34_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HEX5564188.1"
                     /translation="MFGRKRKLKKEFDEKLYSLMLATKEDWEKAKSVEGLLRDYDEEV
                     IVSRKIAESKYFYLFKEAKARQLWNDGPK"
     gene            2815..3324
                     /locus_tag="VFX34_04450"
     CDS             2815..3324
                     /locus_tag="VFX34_04450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564189.1"
                     /translation="MEIRKLIPKDAEAYLKIRLQALQDCPEAFGSSFEEEKEDTAEKY
                     RTRFSTHQPVITFGAFDEEEHLMGVVTLRREHLMKLKHRANVVAMYVKKEKWGAGVGK
                     ALLDVLLTEVRSVSGLEQIYLTVVTTNERAIRLYKSAGFEVFATENRALKVGADYFDE
                     HHMVLFLLS"
     gene            3519..3782
                     /locus_tag="VFX34_04455"
     CDS             3519..3782
                     /locus_tag="VFX34_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HEX5564190.1"
                     /translation="MKLAGILFIVGLFLLLFLMDNKYVHKVLERISIYWFRFAFAFLL
                     LFAMNIIGGFFGFFIPVNIASGIVIAVLGIPGIASIFAFAFFL"
     gene            4006..>4094
                     /locus_tag="VFX34_04460"
     rRNA            4006..>4094
                     /locus_tag="VFX34_04460"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1249 a          816 c          997 g         1032 t
ORIGIN      
        1 aaacgagtgc ggcaaaaata tttgcaaaaa cgatcaactg cgaacaggct ccggtcgcgg
       61 aaccatgtaa tgaatgcccg acctgcctta gcattactga gggctccaat acagatgtca
      121 ttgaatttga cgctgcgtca aattcacgtg tagaggagat gcgggatatt atcgaaaagg
      181 tacggttcgc tccgtccaac gcccgcttta aggtctacat cattgacgag gttcatatgt
      241 tatccaactc ggcatttaac gccttgttga aaacattgga agaaccgcct gctcatgttg
      301 tctttatttt agcgacaact gagccttata aacttccatt gaccatcata tctagatgtc
      361 aacggtttga tttcaagcct cacacgatat ctgatattat tgcaaggctc cgaaaagtac
      421 ttgacgattc cggaatcaaa tcagatgagg gttcattgaa aattattgcc caggcggcat
      481 caggcggaat gcgggatgca ctcagcatac tagaacaggt tgtctccttt agcggcgatg
      541 aactgactgt ggaggatgct cttctcgtga caggagccat cggagaagac attttctatc
      601 aactcgccga agcgctttta ggaaaggatg cgggaacagc actgtccctg cttgatcaat
      661 tgattgctga agggaaagat gtttccagac tcgccgagga tatgatcacg ttcttccggg
      721 atctgctctt gttgcgcact gcgccggcgt taaaagattt acttgaactc gttacaggtg
      781 atgagcgctt tgaggaaatg gcaaagcaat ttaatccgga tgttctgtat tcttatattg
      841 atattctttc aaaaacacag caggagatgc gcttttctaa ctatgcaaag gtataccttg
      901 aatccgccct cctgaaaatg gtcaatgctg cccaagacct gtctacagga acggcagcac
      961 aaagcgggga tctcgtggag aaagtgcaaa atctcgaacg gatgattgcc gaactgcagc
     1021 gtaaattagc tgagggagtt ccagtggcgg cagtccctgc ggatacggca cagccacgaa
     1081 aaccagcctc cagaccaaca agttcgtata aaatacctgt tggaaagata aatgaagtct
     1141 tgaaagaggc gacaaagcaa gacatcatga cgatacgtga acaatgggcg aacatgatgc
     1201 agacgatgca aaaatcgcac gctgcacttc tggaggaaac ggaaccggta gcggcatctg
     1261 agcgtgcgtt tgtgttaaaa ttcaaatatg aaatccattg cggaatggct tctgataatc
     1321 catccttaca atcgagcctg acacaagcat tactttcgag aacagggaag gcctatgatg
     1381 ttctttttgt tccggaagag ggctggctga aagttcgaga ggattttatc cggaatagcg
     1441 ggttggcaaa acagagtgat caacaagcag aggatgaaca aacagccgac gaaatgccat
     1501 cctttatgga agaagcagag gcttcaacct ctgatccact cgtatcggaa gcagaaaaac
     1561 tattcggcaa ggaattcgtt gaagtatatg atgactaatt attccgagga ggaactgaca
     1621 tgcgtggtat gggaaatatg caaggtatga tgaaacaaat gcaaaaaatg caaaagaaaa
     1681 tggcggaggc acaagaaaaa ctaggtgaag agcgcctgga aggtacagct ggaggcggaa
     1741 tggtaaaggt tgttgtatcc ggccataaag aagtcgttga cgtagtaatt gatccgtctg
     1801 tcgtagatcc ggaagacgta gaaatgctgc aagacctttt gattgttgca acaaatgatg
     1861 caatgtcgaa agcggaagaa ttaacgaact ccacgatggg gcaattcacg aaaggattga
     1921 accttcctgg catgttctag gaggaaagat gaatcgtgca ctatccagaa ccgatttcaa
     1981 aactgattga cagttttatg agattgccgg gcatcgggcc gaaaacagcg gcccggctgg
     2041 cattttttgt tttaagtatg aaagaagata atgtactaga gtttgcgaaa gcgctagtcg
     2101 atgcgaaacg gaatcttcaa tattgctcca actgcgggca tattaccgac gtagatccat
     2161 gtcaaatctg ccaggataag agcagagatg gctctatgat ctgtgttgtc caagatccaa
     2221 aagacgtgat cgcaatggaa aagatgcggg actataacgg gtactatcat gtattgcatg
     2281 gggcaatctc tccaatggat ggaatcggtc cggaagatat caatgtccca tctctcttaa
     2341 ctcgattaca gaatgaagaa gtacaagaat tgattttggc gacaaaccct accattgaag
     2401 gcgaagcaac ggcaatgtat atttcccgtc tcgtaaagcc ttcgggaatt aagacaacta
     2461 gaatagccca cggtcttccg gttggcggag acctcgaata tgcggatgaa gttacacttt
     2521 ccaaggcact cgaagggcgt cgtgaactgt aaagggggca acgccaatgt tcggacgaaa
     2581 aagaaagtta aagaaagagt tcgacgagaa gctctactcc ttaatgttgg ctacaaaaga
     2641 ggactgggaa aaggcaaagt ccgttgaagg gctactacgt gactacgacg aagaagtgat
     2701 agtcagtaga aagatagctg aaagcaagta tttctatcta tttaaagaag cgaaagctag
     2761 gcaactatgg aatgacggac caaaataaca atggaagcat tggaggcaat gcagttggaa
     2821 atacgaaaac tcattccgaa ggatgcggag gcatacttga aaatccgcct tcaagcattg
     2881 caagactgcc ctgaagcatt cggctcaagc ttcgaagaag agaaagagga tacagctgag
     2941 aaatatcgaa ccagattttc aactcaccaa cctgtcatca cattcggtgc atttgacgaa
     3001 gaggaacatc tgatgggcgt tgtcacgctg cgtagggaac acctaatgaa gctgaagcat
     3061 cgtgctaacg tcgtcgccat gtatgtgaaa aaggaaaagt ggggcgcagg agttgggaag
     3121 gcacttttag atgttttact cactgaagtc agatcagtaa gtggcctcga acagatttac
     3181 ttgactgttg ttacaacgaa tgaacgagcg attcgtttat ataaatcggc tggatttgaa
     3241 gtgttcgcta cagaaaatag agcactcaag gtgggcgccg actatttcga tgagcatcat
     3301 atggttcttt ttcttctatc ataaaagtta tgcctccaga aaaaatgttc tggaggtttt
     3361 ttaattttga cccatcgtcc aaatagacac acggtaagcg gcaaggattc taattcaatc
     3421 tatatagtgt ggctgtctcg tttcattaaa taaggacata atttaatcta ggtaatgcat
     3481 ataatgggct attacaatgt taagaaggag cgttacacgt gaaacttgca ggcatcctat
     3541 ttattgtagg tctttttctt ttactgtttt taatggacaa taaatatgta cacaaagtac
     3601 tggaacgtat ttcaatctat tggttccgat tcgcatttgc atttctattg ctgtttgcaa
     3661 tgaacattat tggaggattt ttcgggttct ttattcccgt caatatagct tccggcattg
     3721 tcatagccgt gcttggcatc ccaggaatcg catctatttt tgcgttcgca ttttttcttt
     3781 aaaaaaagat gtcattttag tgttgacgtg cgtacaataa tgttgtattc tattaaaggt
     3841 cgctgctaca gcgaacgaca tgaaccttga aaactgaaca gcaaaacgtc aagcaaataa
     3901 agtctgggcg ccgaccccgt cggtgaaaca gacacaacga atctccggat tcaaaattga
     3961 catcattatg atgccagcaa aattatgagc aaatcataat tctcttttat ggagagtttg
     4021 atcctggctc aggacgaacg ctggcggcgt gcctaataca tgcaagtcga gcggacggat
     4081 gggagcttgc tccc
//
LOCUS       DASULI010000206         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_673608, whole
            genome shotgun sequence.
ACCESSION   DASULI010000206 DASULI010000000
VERSION     DASULI010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673608"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /locus_tag="VFX34_04465"
     CDS             complement(<1..2069)
                     /locus_tag="VFX34_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5564191.1"
                     /translation="MDIRIEEPLWLLLLVPIALYMAYAWKSSNMRLASKGTILYILRS
                     LVILCIVFALTVPYMLLPDDEEQILFIVDRSVSVEEAGQTAEQWIADSLKARNENQSV
                     GIYSFAESFRTDIRLTDTKFEVPKLETMDSNGSTNIANALDLSAAVAKSGVATRIVLL
                     SDGLETEGSVEELLPKYENSHVQIDTVVLRRTNAADASISLFESPRTAFEGERQLLRV
                     EVESSTRTAGQLLIYLNDEEIISEQVVLDEGANQFAFHHETSGTGLLKYEAKLIVPDD
                     GLLENNRMLSVTMLEQSPRVLVVETERSPSVIPSLLDRNAMDVDVVDAEELPETLSGY
                     LGFSAIIFDNVPGHLVGEHKMTVIEQAVKSFGTGFMMIGGDESYGLGGYFKSPIERLL
                     PVEMEVKGKEQLPSLGLVITMDRSGSMSGSKIVIAREAAARAVELLRNDDTFGFTAFD
                     HEIWEVIPVGPLQDKQDAMDQILSIPAGGGTDIFPSVAKAYEDLLDLKLQRKHIILLT
                     DGQSSMPPDYEDIIAEGKKTNVTLSTVAIGTDADSTLLEELAELGGGRFYNAVDESTI
                     PAILTRETSMLTRTYIEDDPFYVSLGNVPEWTSIFREGVPQMNAYIATTPKGTATIVA
                     ESPKEDPVIAEWMYGLGRTIAFTSDSTGKWSGDLARWEGYSDFWNTAVARLLPSYEDV
                     PYLITHDR"
     gene            complement(2053..>3344)
                     /locus_tag="VFX34_04470"
     CDS             complement(2053..>3344)
                     /locus_tag="VFX34_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564192.1"
                     /translation="LNYEHEHLGRSLEFVRSIASQKGADVHVFTDYLDRSSFMESESG
                     ITWTVHNNDQPVDNISIGKFGAVHTKDGTEAIVKLSNQTLSKRTGKVIVNNGLTGGKL
                     TEQEFSIDEKDDTLLSFRNLPELPAFHLHMEVEDGYSVDNEAYIIVGNESSEVIVDNQ
                     LHELVKKAFEAIGLSVSSGAINEMTAARDHAMIVTNETSFLEEGTEPILLIGRNDSTA
                     EPVTGTVESTNDALFSIASIADVYISALYPPFEGYSTLAAVDGEPFIQRSPRGDIIVL
                     TDIGLTDWPLHPSFPLFFWSSMEMLRSGSDVAGTFLPNERKALLTGSGTEGIEVFTID
                     DKYVSSYPSGGSFVAPSRPGIYKLMDGGTERYLTVQLEQDEKSVAYGSSYKLGSDMSD
                     ENGKEEGKQMIGWLFLLPVLLLLLIEWEVQRHRGYPN"
BASE COUNT          837 a          908 c          717 g          882 t
ORIGIN      
        1 cggtcatggg tgatcagata aggaacatcc tcataagacg gaagcaatcg agctacagct
       61 gtgttccaga agtctgaata gccctcccat cttgccagat cacccgacca ttttccggtc
      121 gaatcggagg tgaaggcaat cgtgcgtcct agtccgtaca tccattccgc aatgaccgga
      181 tcctcctttg ggctttccgc tacgattgtc gccgttcctt tcggtgttgt cgcaatatag
      241 gcattcattt gggggacgcc ttcccggaag atggacgtcc attccggaac gttgccaagc
      301 gaaacataga atggatcatc ctcgatgtat gtccgtgtca gcatcgatgt ttcccgggtc
      361 aatattgcgg gaatcgtcga ctcgtcaacg gcgttgtaga accgcccgcc gccgagttcc
      421 gccaattcct ccaatagtgt gctatccgca tcggttccga tcgccactgt cgacaatgtg
      481 acattcgtct ttttaccttc cgctatgatg tcttcatagt ccggaggcat tgacgactgg
      541 ccatccgtca acaggatgat atgcttccgt tgaagcttca aatccaacag gtcttcatat
      601 gcttttgcga cagatgggaa aatgtcggtt cctcctcctg ccggaatgga aagaatttga
      661 tccatggcgt cctgcttatc ctgtaaaggt ccgacaggga tgacttccca aatttcatgg
      721 tcgaatgcag tgaaaccgaa tgtatcatca tttctgagca actccaccgc tcgggcagcc
      781 gcttccctcg caatgacaat cttcgacccg gacatactgc cggaacggtc catcgtaatg
      841 actagcccaa gggatggaag ctgctctttc cccttcactt ccatttcgac tggcaataaa
      901 cgctcgatcg gtgacttgaa atagccccct aagccatagc tttcatcgcc cccgatcatc
      961 atgaatcccg taccgaagct tttgaccgcc tgctcaatga cggtcatttt atgttccccg
     1021 acaagatgcc caggtacatt gtcaaaaatg attgcgctga agcctaaata accggataat
     1081 gtttcaggta attcctccgc atccactacg tcgacgtcca tcgcgttcct gtcgagcaat
     1141 gacgggatga cggaggggct cctttcggtt tctacgacta gcactctcgg ggactgctcg
     1201 agcatcgtca cggaaagcat ccggttgttt tcaagaaggc cgtcgtcagg tacgatcagc
     1261 tttgcctcat atttcaatag acccgttccg cttgtctcat gatggaatgc aaattgattc
     1321 gcaccttcat caagaaccac ctgttcactg atgatctcct cgtcattcaa gtagatcagc
     1381 aattggcctg cggttcgggt ggatgactcc acttccacgc gcaataactg cctctcccct
     1441 tcaaaggcgg tccttggcga ctcgaataaa gagatggaag catctgctgc attcgttcgc
     1501 cgtaatacga ctgtatcgat ttggacatgg ctgttttcat atttcggcaa aagctcctct
     1561 acggatcctt ccgtctccag tccatcggag agtaagacga tcctcgtcgc tacacctgat
     1621 tttgcaaccg ccgcggataa atcaagcgca tttgcaatat ttgttgaacc attcgagtcc
     1681 atcgtttcca gcttcggcac ttcaaacttg gtatctgtca gtcggatatc cgtccggaat
     1741 gattcggcaa aagagtaaat tccgacggac tgattctcgt ttctcgcctt taagctatct
     1801 gcaatccatt gttcagctgt ttggcccgcc tcttccacgg atacggatcg atcgacgatg
     1861 aataggatct gctcctcgtc atcgggcaac agcatataag gtacagtaag ggcaaaaacg
     1921 atacagagaa tgaccaggct ccttaggatg tagagtatag tccctttact cgcaagccgc
     1981 atattggaag atttccaagc atacgccatg taaagggcta tcgggacaag cagcaagagc
     2041 cataaaggct cctcaattcg gatatccacg atgtctctgc acctcccact ctatcaacaa
     2101 aagaaggagg actggcaata ggaaaagcca tccgatcatt tgcttccctt cctctttccc
     2161 attttcatca gacatatctg acccaagttt ataactggag ccataggcaa ccgatttctc
     2221 atcctgctcc agttgaacgg ttaaatacct ttccgtcccg ccgtccatca atttataaat
     2281 gcccggtcta gatggggcga caaaacttcc gcctgaagga taagatgaaa catacttatc
     2341 atcgattgtg aagacctcta tcccttccgt accgcttcct gtcaataacg ctttcctctc
     2401 attcgggaga aatgttccag ctacatcact tccggaacgc agcatctcca tcgagctcca
     2461 aaaaaacaac gggaacgagg gatgcaatgg ccaatcggtc aaaccgatat ctgtcaagac
     2521 gataatatcg cctctcggtg atcgttggat gaatggctcg ccatctactg ctgccaaagt
     2581 tgaatatcct tcaaatggcg gatagagggc acttatataa acgtcagcaa tcgaagcgat
     2641 tgagaacaat gcatcattcg tagattccac cgtacccgta accggctcgg ccgttgaatc
     2701 attcctccct atcaacagaa tcggttcagt tccttcctcc aaaaacgacg tttcattcgt
     2761 cacaatcatt gcatggtccc ttgcagccgt catttcattg atggcacccg aactgacaga
     2821 cagccctatc gcttcaaaag cctttttcac caattcatgc agctggttat cgacaatcac
     2881 ttccgaagat tcattgccaa cgatgatata cgcctcattg tcaacggaat acccatcttc
     2941 cacttccata tgcaaatgga atgccggcaa ctcaggcagg ttcctgaatg aaaggagagt
     3001 atcatctttt tcgtcaattg aaaattcctg ttcagtcaat ttaccgccag tcaacccatt
     3061 attcacaatc accttgccag ttcgttttga aagtgtttgg ttggaaagct tcacaatcgc
     3121 ctctgttcca tcctttgtat gtacggcacc gaatttaccg atggaaatat tgtctaccgg
     3181 ttgatcattg ttatggacag tccaggtgat gccactttcg ctttccatga acgatgaccg
     3241 gtcaagataa tcagtgaaaa catggacatc cgcccccttt tgcgaggcaa ttgatcggac
     3301 aaactcgagg gaccggccaa gatgttcatg ttcatagttc aatt
//
LOCUS       DASULI010000207         3553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1485266,
            whole genome shotgun sequence.
ACCESSION   DASULI010000207 DASULI010000000
VERSION     DASULI010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485266"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFX34_04475"
     CDS             complement(<1..464)
                     /locus_tag="VFX34_04475"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5564193.1"
                     /translation="MKIINAADFTVEIEQRNKQSMDLEFDRKVLSIINDVRINGDAAL
                     HLYTERFDGVKLESFIVTDDEYDEAYTLVSQSFIASLLKAKQRITDFHEEQKERSWFI
                     RQEKGVMLGQQVTPIERVGIYVPGGKAAYPSTALMNVIPAKIAGVGHIAIATP"
     gene            complement(461..1096)
                     /gene="hisG"
                     /locus_tag="VFX34_04480"
     CDS             complement(461..1096)
                     /gene="hisG"
                     /locus_tag="VFX34_04480"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX5564194.1"
                     /translation="MDYLTVAMAKGRIANQAIALLEEAGILFSDFDDKSRKLIFKDDA
                     GSIKLIFVKAVDVPTYVELGAADIGVIGKDVLMEDPKDVYELLDLLIGRCKLSVAGYP
                     EKKTTELTQLTVASKYPNIARLHFEQKGIRTDIIELNGSVELAPLIGLADVIVDIVET
                     GTTLKENGLVVLEDIAEVSARLIVNKASFAIKTERIQNFIADVKAGLEVYS"
     gene            complement(1096..2073)
                     /gene="hisZ"
                     /locus_tag="VFX34_04485"
     CDS             complement(1096..2073)
                     /gene="hisZ"
                     /locus_tag="VFX34_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX5564195.1"
                     /translation="MNPDLIEVEKFEQVISTLSERFSTYGFQRIKTPAFERYDLYSKV
                     TGSVNRKEMVKVIDPSGEVLVLRPDVTIPITQELSHAHRKLTSEHRFYYIQEVFRRSS
                     DPTEKIERTQAGIEYFCKSSPGVDADTILLALNTMKDLKFTDIKIEIGHTAFFQEMIS
                     QLPLTELQLNQLKACIQAKNSVEIGTLLENVDADTAIKNAVAQIPTLYGKPEEVLKRA
                     EEISTTSRMRETLSYIGEVCRRITANGLEEHLVIDFGLINRMDYYSGILFQGYVGRFG
                     KPVLMGGRYDKLGHEFGADLPAIGFACEIESLLKASDNQKMFNETKGDA"
     gene            2173..2967
                     /locus_tag="VFX34_04490"
     CDS             2173..2967
                     /locus_tag="VFX34_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HEX5564196.1"
                     /translation="MFDYHMHSSFSGDCNVDMEDMVKGSLAKGVAEICFTDHIDYDYP
                     DASIEFDFDKRKYEERIDELRKRYAGQIRIKKGVEIGVQPHLLERYDEMLKAESFDFI
                     ICSMHTVDRKGLHYGDLFKGITVEEASQKYYDELLYCVKNFTNYSILGHVDLIKRYAP
                     EVVDNDFHDVLREIFNVIIPAGKGIELNTSGVRYGLPNGLPSDDVLKLYKECGGEIIT
                     LGSDAHKPEDISFDFKESLERLQSIGFDYIAAFEGLKPTFHPIDKL"
     gene            complement(3055..>3553)
                     /locus_tag="VFX34_04495"
     CDS             complement(3055..>3553)
                     /locus_tag="VFX34_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5564197.1"
                     /translation="WMTDGLKPLADGSFEYAIILGAKVNGETPSLTLRYRLEKALSYA
                     ETYPDVKFILSGGQGQDEDIAEGEAMRRYLTENGVEEERLLLETESTSTYENLLYSKR
                     MLPENVSGVTIITSDFHLARARMIADSLGLETDALPAKTPQSVEAKHHIRERLALIKT
                     MIVGK"
BASE COUNT          880 a          846 c          779 g         1048 t
ORIGIN      
        1 ggtgtcgcaa tcgcaatatg gccgacccct gcgattttag cgggaatcac attcataagt
       61 gctgtggatg gatacgccgc tttcccgccc ggtacgtaaa tgcctacacg ttcaattgga
      121 gtcacttgct ggccaagcat aacgcctttc tcctgtctaa tgaaccatga gcgctctttc
      181 tgttcctcat gaaaatcggt gattctctgt ttcgctttta aaagtgatgc gataaagctt
      241 tgcgaaacga gcgtataagc ttcatcgtat tcatcatccg tgacaatgaa gctctccaat
      301 ttcacgccat cgaaacgctc ggtatacaga tgaagagccg catccccgtt tatacgaacg
      361 tcgttgatga tcgacaatac ttttcgatca aactccaaat ccatggactg tttgtttcgt
      421 tgctcaattt ccactgtaaa gtcggcggcg ttgataattt tcatgaatac acctccagcc
      481 ctgccttgac gtcggcaatg aaattctgaa tacgctccgt cttgattgcg aagcttgctt
      541 tgttcacaat caaccttgca ctcacttccg caatatcttc gagaacaact aacccattct
      601 ccttcaatgt tgtgcctgtt tcgacgatgt cgacaatgac gtccgccagt ccgatgagcg
      661 gcgccaattc aaccgacccg ttcagttcga tgatgtcagt gcggatgcct ttttgttcga
      721 aatggagtct tgctatattc ggatacttgg aagcaaccgt caactgggtc agttcggtgg
      781 tctttttttc cggatatccc gcgacagaca atttgcatct ccctatgaga agatctaata
      841 attcataaac gtccttcgga tcttccatca gaacatcttt cccaatcacg ccgatatctg
      901 ctgcgcctag ttccacgtat gtcggcacat cgaccgcttt cacgaatatc aacttgatgg
      961 atcccgcatc atccttgaag atgagtttcc tgcttttatc gtcaaagtcc gagaagagaa
     1021 tgcccgcttc ctccaaaagc gcaatcgcct gattcgcaat ccttcctttt gccatcgcga
     1081 ctgtcaaata gtccatcatg catccccctt tgtttcattg aacattttct ggttatcgga
     1141 agccttcaat aaggattcga tttcacaagc gaatccaatt gcaggcaggt cagctccaaa
     1201 ttcatggcct agtttgtcat accttccgcc catcaatacc ggtttgccga agcgtccgac
     1261 atatccttga aaaaggatgc ccgagtagta atccatccgg ttgatcaacc cgaaatcgat
     1321 gacgagatgc tcttccaatc catttgccgt tatacgccgg caaacttcac cgatatagga
     1381 tagtgtttcc cgcatgcggg atgtagtgga aatctcttct gcgcgcttca gcacttcttc
     1441 cggctttcca tagagagttg gaatttgcgc gacggcattt tttatagccg tgtctgcatc
     1501 cacattttca agcagtgtgc cgatttcaac actgtttttc gcctgtatac atgctttcaa
     1561 ctgatttagt tgtaattcgg ttaacggaag ttgggaaatc atttcttgga agaaggccgt
     1621 atgcccgatt tcgattttga tgtccgtgaa cttcaaatct ttcatcgtat ttagcgccaa
     1681 taaaatcgta tccgcatcga cccctggcga actcttgcaa aaatattcga tccccgcttg
     1741 cgtccgctcg attttttcag ttgggtcgga tgacctccga aacacttcct gtatataata
     1801 aaagcggtgt tcacttgtca gcttgcggtg agcgtgagac aattcctgtg taatcggtat
     1861 cgtcacatcg gggcgaagga caagaacttc cccagatgga tcgatgactt tcaccatctc
     1921 tttccggttg acggaacccg tcactttgga ataaagatca taacgctcaa aagccggtgt
     1981 cttaatccgt tggaacccat atgttgaaaa tcgctcgctt aacgtactga tcacttgctc
     2041 gaacttttcc acttcaatta aatcgggatt catttgcata ccacctttac ttcatcactt
     2101 tactacgtta aagttataac gtactataaa actatattta tcgtttgtca accgatagga
     2161 ggaatttttt taatgtttga ttaccatatg cacagttcat tttcaggaga ttgcaatgta
     2221 gatatggaag atatggtcaa aggatcgctt gcaaaaggcg ttgcggaaat ctgcttcacg
     2281 gatcatatcg attatgatta tccggatgca tcgattgaat ttgatttcga taaacggaag
     2341 tatgaagagc ggattgatga actgcgcaag cgatatgccg ggcagatccg catcaagaag
     2401 ggcgtcgaaa ttggggtgca gccgcattta ttggaacgct atgacgaaat gctaaaagcg
     2461 gagtcgtttg atttcatcat ttgctcgatg catacagttg atcggaaagg tttgcactac
     2521 ggagatcttt tcaaagggat tacggtggaa gaagcctctc agaagtatta cgatgaattg
     2581 ctgtattgcg tgaagaactt tacgaactat tccattttag gacatgttga tttgattaaa
     2641 cggtatgctc ctgaagtcgt cgacaacgac ttccatgatg tgttgcgtga aatatttaac
     2701 gtaattattc cagctggaaa agggattgag ttgaatacgt ccggtgtacg ttatggtctg
     2761 ccgaacggat tgccgagtga tgatgtgttg aagttgtata aggaatgtgg cggggagatc
     2821 attacgttag gatctgatgc acataagccg gaagatatct cgtttgattt caaggagtcg
     2881 cttgagcgat tgcaatcgat cggctttgat tatatcgcag cctttgaggg gttgaaaccg
     2941 actttccatc cgattgataa gctttgacgt aatagtcgtg ctcgctttga gcatggaaca
     3001 ttttattttg agcgcaaaac agcccgcttt ggatgcgggc tgtttgcttt gatttcactt
     3061 tccaacaatc atcgtcttga tgagggcaag tctttccctt atgtggtgtt tagcttccac
     3121 tgactgcggt gtcttcgccg gcaatgcatc tgtctctaga ccaaggctgt ccgcaatcat
     3181 ccgtgcacgc gcgagatgaa aatcactcgt gatgatcgtg acaccactca cattctccgg
     3241 aagcattctc ttcgaataca ggaggttctc gtaagtcgat gtcgactcgg tttcaagcag
     3301 gagcctttcc tcttccacgc cattctccgt caagtatcga cgcatcgctt ccccttccgc
     3361 aatgtcttca tcttggccct gaccgcccga taagatgaat ttcacatcag ggtaggtttc
     3421 cgcgtaagat aatgcctttt ccaaccgata gcgcagagta agggaaggtg tctccccgtt
     3481 cactttagcg ccgaggatga tggcatattc aaatgagcca tccgcaagag gtttcagtcc
     3541 atccgtcatc cat
//
LOCUS       DASULI010000208         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_732174, whole
            genome shotgun sequence.
ACCESSION   DASULI010000208 DASULI010000000
VERSION     DASULI010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732174"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFX34_04500"
     CDS             <1..683
                     /locus_tag="VFX34_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX5564198.1"
                     /translation="EMDKEKNRLILSHRAVLQEEKASKKEEVLEGLKEGQVLEGTVQR
                     IASFGAFVDIGGVDGLVHISQLSHEHVEKVSDVLKEGEKVNVKVLSVDRDSERISLSI
                     KETLPGPWENIEERAPKGSVLEGTVKRLVSYGAFVEVFPGVEGLVHISRISHQHIGTP
                     HEVLEEGQKVDVKVVEVNAEEKRLSLSIKDLLEKEENESFGDYEMKEETGFSLSDVIG
                     DQLKKFTE"
     gene            806..2116
                     /gene="der"
                     /locus_tag="VFX34_04505"
     CDS             806..2116
                     /gene="der"
                     /locus_tag="VFX34_04505"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5564199.1"
                     /translation="MNKPTVAIVGRPNVGKSTIFNRIVGERISIVEDVAGVTRDRIYS
                     SADWLSHDFNLIDTGGIDIGDEPFLEQIRAQAEIAIEEADVIIFLTNGREGVTDADEH
                     VAKILYKTKKPVVLAVNKIDNPDMRDMIYDFYSLGFGDPYPISGSHGIGLGDLLDAVA
                     ASFPEAGEEVEEDDVIRFSLIGRPNVGKSSLVNALLGEERVIVSDVAGTTTDAIDTTY
                     EFEGQKYKIIDTAGMRKKGKVYENMEKYSVLRALKAIDRSDVVLIVINGEEGIREQDK
                     RIAGYAEEAGKGIMFVVNKWDAVEKDDKTMNRFTEDIRKNFLFLDYAPIAFVSAKTKQ
                     RVTTLFDQIKLISENHAMRIQSSVLNEVIEDAVARNPAPSDKGRRLRIYYATQVAVKP
                     PVFIVFVNEPELMHFSYERFLQNRLRESFGFEGTPIRLITRARS"
     gene            2203..>2520
                     /locus_tag="VFX34_04510"
     CDS             2203..>2520
                     /locus_tag="VFX34_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5564200.1"
                     /translation="MERVSVIGAGSWGTAIAFVLAENGHDCLLWTRRAEQAAEINERH
                     TNASYLPGITLPANLKAISNLEKAVQHAEMIVIAVPTKAIRSICAEINELAGNSKLII
                     HVSK"
BASE COUNT          748 a          499 c          655 g          618 t
ORIGIN      
        1 tggaaatgga caaggagaaa aatcgcctca tcctttcaca ccgcgcagtc cttcaagagg
       61 aaaaggcgag caaaaaagaa gaagtgcttg agggacttaa agaaggacag gtgcttgaag
      121 ggacagtcca acgcatcgcc tcgtttggag ctttcgttga tatcggcggc gttgacggac
      181 tcgtccatat ttcacagcta tcccatgaac atgtggaaaa agtgtccgat gtcctgaaag
      241 aaggcgaaaa agtgaatgtg aaagtccttt ccgtagatcg tgattcggag cggatttcat
      301 tatccattaa agagacattg cctggaccat gggaaaacat tgaagaaagg gcgccaaaag
      361 ggtctgtgct ggaaggcact gttaagcgtc ttgtctccta tggcgcattc gtcgaagtat
      421 tccctggcgt tgaaggcctt gtccatattt cacggatttc ccaccagcat atcggaaccc
      481 ctcatgaggt tttggaggaa ggacaaaaag tcgatgtgaa agttgtcgaa gtgaatgcag
      541 aagagaaacg actttccctc agcatcaaag accttcttga gaaggaagaa aacgaatcat
      601 tcggcgatta tgaaatgaag gaagagacgg gcttctcctt gagcgatgtc atcggagatc
      661 aattgaaaaa gttcactgaa tgatatttgt caaattatcg aacagacgaa tgaatcatat
      721 ctgtatgggt gatggtgctt acataggcat catcatcttt tttgtgtata atactcaaga
      781 gatatattgg aagggtgtta aatgaatgaa taaaccaacc gtagcgatcg ttggaaggcc
      841 gaacgtagga aagtcgacta tcttcaaccg tatcgttgga gaacgtattt cgatcgtcga
      901 ggacgttgcc ggcgtgacga gggaccggat atacagctcg gcagattggc tctcgcatga
      961 tttcaatctg atcgatacag gcggaatcga catcggtgat gagccattcc tggagcagat
     1021 cagagcgcag gcggaaattg cgatcgaaga agcagatgtc atcatctttt tgacaaatgg
     1081 ccgggaaggt gtgaccgatg ccgacgagca tgttgctaaa atcctttata aaacgaagaa
     1141 acctgtagtt ttagctgtca acaaaattga taatccagat atgcgcgata tgatctatga
     1201 cttttattcg ctcggctttg gagatccgta tccgatttcc ggttcccatg gaatcgggct
     1261 cggggatttg ctggacgcag ttgcagcaag ttttccggaa gccggggaag aagtcgaaga
     1321 ggatgatgtc atccgttttt cattgatcgg tagaccgaat gtcggtaaat cttctctcgt
     1381 aaacgccttg ctcggagaag aaagggtcat cgtcagtgac gttgccggta cgacaacaga
     1441 tgcgatcgat acaacgtatg agttcgaagg acagaagtat aagatcattg atacagccgg
     1501 tatgcgaaaa aaaggaaaag tgtatgaaaa catggagaaa tacagtgttt tacgtgcatt
     1561 gaaagcaatc gaccgatcag atgtagtgct cattgtcatt aacggcgagg aagggatccg
     1621 tgaacaggat aaacggattg cgggctatgc agaagaagcg ggaaagggca tcatgtttgt
     1681 tgtgaataaa tgggatgctg tcgagaaaga cgataaaacg atgaatcgtt tcactgaaga
     1741 tatccgcaag aatttcctgt tcctcgatta tgcaccgatc gctttcgttt cggcgaagac
     1801 gaaacagcgc gtcacaactc tgttcgatca aataaaactg atcagtgaaa atcatgcaat
     1861 gcgcatccaa tcgagcgtct tgaatgaagt gatcgaggat gctgttgcgc gcaatccagc
     1921 tccttcagat aaaggacgca gactgcgcat ctattatgcg acacaagtgg cggtcaagcc
     1981 tccagtcttc atcgtatttg tcaatgaacc ggaattgatg catttctcat atgaaaggtt
     2041 tttgcaaaac cgcctgaggg agtctttcgg atttgaaggg acgccgatcc gactgattac
     2101 acgtgcaaga agctgataaa ccgaattcag ccactatttt tgtactgaaa tcgaagcatc
     2161 ggatacagaa attttttcgt aattactaat gaggtgaatg tattggaaag agtgtcagtg
     2221 atcggggcag gaagctgggg gacagcaatc gcattcgttc ttgctgaaaa cggccatgac
     2281 tgcctgcttt ggacaaggag ggcggaacag gcagccgaaa tcaacgaacg acatacaaat
     2341 gcctcatatt taccggggat cacattgcct gccaatctga aggcgatttc caatttggaa
     2401 aaggccgttc aacatgcaga gatgattgtc attgccgttc cgacaaaagc catccggtct
     2461 atttgtgctg aaatcaacga gttagcaggc aattcgaagt tgatcatcca tgtttcaaag
//
LOCUS       DASULI010000209         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1252218,
            whole genome shotgun sequence.
ACCESSION   DASULI010000209 DASULI010000000
VERSION     DASULI010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252218"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VFX34_04515"
     CDS             <1..1352
                     /locus_tag="VFX34_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX5564201.1"
                     /translation="IRLAPEHIAYVINHAEDEILLVDDNLFPHLEKLAPMLKTVKHYV
                     IMGDSAEVPETTLENVHSYEALLAEASDQYDYPEDLDENTPAGMCYTSATTGNPKGVV
                     YTHRGIVLHSFALGLADAMGLREGDVIMPIVPMFHVNAWGMPFAAVFYGSTQVLPGPG
                     FNPQLILDLIEQEKVTLTAGVPTIWLAVLKEQQQNPRNLSSLRGIVSGGSASPKGLIR
                     AFEEGLGVPFYVGYGMTETSPLVSLSVYTSGMEDLPMDERIDIRAMQGLIMPGLEVKV
                     VNEDGEVPWDGKTMGELAVRGPWIASEYYKDDRTAEAFKDGWLYTGDIAVMTDIGYLK
                     LMDRTKDLIKSGGEWISSVDLENALMTHEAVFEAAVIAIPHEKWVERPLACVVLKEGK
                     TADDALKAELLDYLAGQFAKLWVPDDVVFLDEIPKTSVGKFLKAALRDQLKGHVLEV"
     gene            1396..1797
                     /locus_tag="VFX34_04520"
     CDS             1396..1797
                     /locus_tag="VFX34_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564202.1"
                     /translation="MGFFMENRRVYGNSVVNGCESVVKRPESVVKHRESVVNGLSGDK
                     PHLSGDKVIASGDNVSTIGNHHPKGDFQLELQSNGDEFSRVGDKTSRVGDKLPRVGDN
                     TSKVGDKLYSHTPALISSEKGIAYREECSIT"
     gene            1809..3092
                     /locus_tag="VFX34_04525"
     CDS             1809..3092
                     /locus_tag="VFX34_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX5564203.1"
                     /translation="MLNRIPKSETTACSLTVYNDGFALVKDTRSIPQFTENDEVHFVG
                     VARKLKPDSIIVEGLDVSELVYEYDLMDELRIYERYLGRQLTIKDPYTKQDRAYTLLR
                     GNGPIIMQDVETKEIIINPRGEIRFPEIEGGNHVEPTLVWKVAGQRGEEVCVSYLTGG
                     VTWEADYVISMHGDRFDLSGWLSMKNYSGVSFNDAKLRLISGKLSKAGNENEEKSAEL
                     SRRGQQKTEAFSDFHTYTFPRDIDLEDRQQKQLRLLSSTNSKARVIYEINEQTENPDI
                     FIEFDNTVENGLGFPLPAGVFKMYRTNPDDGSAEFIGEYAIKHTEVNEKVRLKSGEAC
                     DIEVKAKKSGKYKEGGYEYTEYEYDICNTKGTPVEAVIRQQVPGHLWTVDESTHEWQK
                     KRDKILLPVHVPVGVEEKVKFTIRHDRSARRTIGF"
     gene            3426..4037
                     /locus_tag="VFX34_04530"
     CDS             3426..4037
                     /locus_tag="VFX34_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX5564204.1"
                     /translation="MENWITEFIGEQGYFAVLLLLLIENIFPPIPSEIILTFSGYATG
                     FTSMTRTGVIIAATIGAVVGAMILYSIGLLVDVARLEKFVDRWGRWLRLSRKDLRRAE
                     KWFEKYGPWTVFFCRLVPLVRSLISVPAGMAHMNFLLFITLTTIGSLIWNTILVTIGF
                     AVGDNWESIVRYMDVYSNIVYVLLAIGGIAVCIGYIRFRRKRA"
     gene            4041..>4271
                     /locus_tag="VFX34_04535"
     CDS             4041..>4271
                     /locus_tag="VFX34_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5564205.1"
                     /translation="MDLFELVKALILGFVEGMTEFAPVSSTGHLIIVDDMWLKTEEFL
                     GKYPANTFKIVIQLGSILAVVVVFWKRLLSLVG"
BASE COUNT         1118 a          898 c         1187 g         1068 t
ORIGIN      
        1 acatccgctt ggcgccggag catattgcgt atgtcatcaa ccacgcggaa gatgaaatcc
       61 tgctcgtcga tgataatctt ttccctcatc ttgaaaagct ggcgccgatg ctcaagacgg
      121 tgaagcatta cgtaattatg ggcgatagtg cggaagtgcc ggaaacgacg ctcgagaatg
      181 tccattccta tgaggctttg cttgcggagg catctgatca gtatgattat ccggaggact
      241 tggatgaaaa cacgcctgcc ggaatgtgtt acacatccgc aacgacgggc aatccgaaag
      301 gggttgtcta cacgcaccgc ggcatcgtcc ttcatagctt tgcgcttggg ttggcggacg
      361 caatggggct gcgagaaggc gatgtcatca tgccgattgt tccgatgttc catgtcaatg
      421 cttgggggat gccttttgcc gcagtattct acggttcgac acaagtgctc ccgggacctg
      481 gcttcaatcc gcagctcatc cttgatttga ttgaacagga gaaagtgacg ctgacggcgg
      541 gcgtgccgac gatttggcta gcggttttga aagagcagca gcaaaatccg cggaatcttt
      601 catcgcttcg aggcatcgta agcggcggct ccgcatctcc gaaaggattg atccgtgcgt
      661 tcgaagaggg gcttggggtt ccgttctacg tcggatacgg catgactgag acatcgccgc
      721 tcgtaagttt gtcggtctac acatcaggca tggaggactt gccgatggac gaacgaatcg
      781 atatccgcgc gatgcaaggg ttgatcatgc cggggcttga agtgaaagtt gtgaatgaag
      841 acggtgaagt gccatgggat ggcaagacga tgggtgaact tgcggtgcgc ggtccttgga
      901 ttgcaagtga atattacaag gacgaccgga cggcggaagc attcaaggac ggctggcttt
      961 acacgggcga catcgccgtc atgacggaca tcggttattt gaaactgatg gaccggacga
     1021 aggatttgat caagagtggt ggggagtgga tttcctccgt cgatttggag aacgcattaa
     1081 tgacgcatga agccgtgttc gaggccgccg tcatcgcgat cccgcatgaa aagtgggtgg
     1141 aacgcccgct tgcctgcgtt gttttgaaag agggcaaaac ggcggacgat gcattgaaag
     1201 ccgaactgct cgactacttg gctggccagt tcgcgaagct gtgggttccg gacgacgtcg
     1261 tgttcctgga cgaaatcccg aaaacatctg tcggcaaatt cctgaaagcc gccctgcgcg
     1321 atcaattgaa aggccatgtg ttggaagtgt gaatttgtgt gttgccaccc actattggta
     1381 gcgggtggtt ttttgatggg attttttatg gagaatcgcc gcgtttacgg caactcggtg
     1441 gtaaacggct gcgaatcggt ggtaaagcgt ccggagtcgg tggtaaagca tcgagaatcg
     1501 gtggtaaacg gtctaagcgg tgataaacct catctaagtg gtgataaagt gatagcgagt
     1561 ggtgataacg tatctaccat tggaaatcat catccaaaag gcgattttca acttgagttg
     1621 cagtcaaatg gtgatgaatt ttcccgagtc ggtgataaaa catcgagagt cggtgataaa
     1681 ctaccccgag tcggtgataa cacctccaaa gtcggtgata aactttattc tcataccccc
     1741 gcattaattt catcagaaaa gggaatagcg tatagggagg aatgttcgat cacataggag
     1801 ggtttaccat gctgaacaga attccgaaat ccgagacgac agcttgttcc ctaactgttt
     1861 acaatgatgg ctttgcgctt gtgaaagata cacgttccat tccgcagttt acggaaaatg
     1921 acgaggtcca ctttgttggt gtggcgcgga agttgaagcc cgattcgatc attgttgaag
     1981 ggctggatgt gtctgagctt gtgtatgaat atgatttgat ggatgaattg aggatttatg
     2041 aaaggtatct tggccgccaa ctgacaatca aggatccata caccaagcaa gatcgggcgt
     2101 acacactgct gcgcgggaac gggccgatca tcatgcagga tgtcgagacg aaagagatta
     2161 tcatcaaccc gagaggcgaa attcgattcc cggaaatcga gggtgggaat catgtggagc
     2221 cgacgcttgt ctggaaagtc gccgggcaac ggggcgaaga agtatgcgtt tcgtacttga
     2281 cgggcggcgt tacatgggaa gcggattatg tcatttccat gcatggcgac cgtttcgatt
     2341 tatcgggttg gctgtcgatg aagaattatt ccggtgtctc tttcaacgat gcgaaacttc
     2401 gcctcatatc agggaagctg agcaaagcag gcaatgaaaa cgaagagaaa agtgctgaat
     2461 tgtcgagacg tggacaacag aaaacggagg ctttctccga tttccatacg tatacgttcc
     2521 ctcgggatat cgacctggag gaccgtcagc agaagcagct gcggcttctg tcatctacca
     2581 attcgaaagc gcgtgtcatc tatgaaatca atgaacagac ggaaaacccg gacatcttca
     2641 tcgaattcga taatacggtg gagaacggac taggtttccc attgccggca ggggttttca
     2701 agatgtaccg gacgaacccg gacgatggca gtgcagaatt catcggcgag tacgccatca
     2761 aacataccga agtgaatgaa aaagtccgtt tgaaatcggg agaagcttgc gacatcgaag
     2821 tgaaggcgaa aaaatcgggc aaatataaag aaggcggtta cgaatacact gaatatgaat
     2881 atgacatctg caatacgaaa ggcaccccgg tcgaagcggt catccgtcaa caagtgcctg
     2941 gccacttatg gacggtggat gaatcgacgc atgaatggca gaagaaacgc gataagattt
     3001 tattgcctgt ccacgttccg gttggcgtcg aggaaaaagt gaagttcacc atccgccacg
     3061 atcgctccgc aagaaggacg attggtttct aatgaataac attccaaact gtgcaattct
     3121 ctaaagaaat gtggaaaaac acccgatgcc gggaatgaaa accggctgca cgggtgtttt
     3181 ttattaacat gtggataaca ttccgtcaaa accccttact tttcagaaat tataaacata
     3241 taaacaatct tacccacagt tttcggtgta taagtccaaa atagcggact tatccacgac
     3301 aagcccattc atactcttcc ttttttctac gtacaatcca tcaggagata ttggaacttc
     3361 caagtgaaga ccgacgtctg ttagaatgga gatatgtcgt attttttacg ggcaaggaga
     3421 gtgcaatgga aaactggata acggaattca taggggaaca aggatatttt gcagtgcttc
     3481 tcctactatt aatagaaaat atattccctc cgattccgtc agaaatcatt ttgactttta
     3541 gtggatatgc gacaggcttt acatcgatga cgaggacagg tgtcatcatc gctgctacaa
     3601 tcggtgcagt cgtcggtgcg atgattctat atagcatcgg cttgcttgtc gacgtagcga
     3661 gactggagaa gtttgttgac agatggggac gctggctgcg gctgtcgagg aaggatttgc
     3721 ggagagctga aaaatggttt gaaaagtacg gtccatggac agtgtttttt tgccgtctcg
     3781 ttccgctcgt gcgcagtctg atttcagttc cagccgggat ggcgcatatg aatttcctgc
     3841 tgttcatcac actgaccacg attggaagct taatttggaa caccattctt gttacaatag
     3901 ggtttgcagt cggtgataat tgggagtcga tcgtccgtta tatggacgtc tattcgaaca
     3961 ttgtgtatgt gttgttagca attggcggaa tcgcggtttg catcggttac atccgtttcc
     4021 gcagaaagag ggcttagtag atggatttat ttgaattagt aaaagcgttg attcttggtt
     4081 ttgtggaagg gatgacggag tttgcgccgg tctcctcgac aggccatctc attatcgtgg
     4141 acgatatgtg gttgaagacg gaagagttcc taggaaaata tccggctaac acatttaaaa
     4201 tcgtcataca gctaggatcg atattagcag ttgtcgtcgt gttctggaaa aggttgctca
     4261 gtttggtcgg c
//
LOCUS       DASULI010000210         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_382452, whole
            genome shotgun sequence.
ACCESSION   DASULI010000210 DASULI010000000
VERSION     DASULI010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382452"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFX34_04540"
     CDS             <1..546
                     /locus_tag="VFX34_04540"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5564206.1"
                     /translation="FHSKMDRFNITFQQIPSKIAAVGTQTAAALEKIGFTVEFIPSIF
                     SADVFVKQFNPTEDRKLRVLFLKGNLAGSIIREELPFQVEEWTVYETGPQPDSVTSII
                     DLLNTEESITVLFASPSAVHVFEEKAVPVIGWSGYTVGAIGHVTEKALLEAGANVDIK
                     PEIYTLKELVNALARRKEEIQ"
     gene            543..1523
                     /gene="hemB"
                     /locus_tag="VFX34_04545"
     CDS             543..1523
                     /gene="hemB"
                     /locus_tag="VFX34_04545"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5564207.1"
                     /translation="MTQLQFRRNRRLRNSATLRSMVRETILQKEDFIYPLFIIEGENV
                     KNEISSMPGIYQLSLDRLMEEVDEVVSLGIPSVILFGIPAVKDATGTGAFDDEGIVQR
                     ATRLIKGNHPDLVVIADTCLCEYTDHGHCGVVHGSDVLNDPSLELLAKTAVSQAKAGA
                     DIIAPSNMMDGFVVAIRQALDEEGFENVPIMSYAVKYASAYYGPFRDAAESAPQFGDR
                     KTYQMDPANRMEALREAESDVMEGADFLIVKPALSYLDIMRDVKNNVLLPVVAYNVSG
                     EYSMVKAAAQNGWINEKEIVLETLTSMKRAGADLIMTYHAKDAARWLEGK"
     gene            1524..2816
                     /gene="hemL"
                     /locus_tag="VFX34_04550"
     CDS             1524..2816
                     /gene="hemL"
                     /locus_tag="VFX34_04550"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5564208.1"
                     /translation="MGKSYEKSKQAFAEAVKLMPGGVNSPVRAFKSVNMDPIFMESGK
                     GPIIKDIDGNEYIDYVLSWGPLILGHADPDVVEGIKKVAETGSSFGAPTLLENELAKI
                     VIDRVPSIEVVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSG
                     VATLGLPDSPGVPEGIAKNTITVAYNDMDGVRAAFEQFGDDIAGVIVEPVAGNMGVVP
                     PEPGFLEGLREITEKNGTLLIFDEVMTGFRVDYNCAQGYFGITPDLTCLGKVIGGGLP
                     VGAYGGKREIMENIAPAGTVYQAGTLSGNPMAMTAGIETLKKLTPASYDHFKKLGDQL
                     EAGFREAATKYNIPHTVNRAGSMIGFFFTNEKVYNYDQAKTSDLELFAEYFRLMAEEG
                     IFLPPSQFEGMFLSSSHTEEHIEQTVQAFHRVFEKLAQ"
     gene            2923..>3269
                     /locus_tag="VFX34_04555"
     CDS             2923..>3269
                     /locus_tag="VFX34_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
                     /protein_id="HEX5564209.1"
                     /translation="MKKLQWNMEEVFYFPENVGVPKDARLVKVKAGFTEKRSEDAVRL
                     SGIYHIAAKVDFAEGQRSEAIPNDAVYVDDVEIEGDTGYFEYAVPLYIDLPPEVEHPL
                     HIEATDIKPTFDG"
BASE COUNT          894 a          719 c          848 g          808 t
ORIGIN      
        1 ttccattcaa aaatggaccg tttcaatatt acatttcaac aaattccctc caaaatagct
       61 gctgtcggta cacagaccgc cgccgcattg gagaaaatcg gcttcactgt tgaattcatc
      121 ccgtccattt tcagtgccga tgtgtttgta aaacaattca atccgaccga agaccggaag
      181 cttcgggtcc tgtttcttaa agggaatctt gcaggcagca tcatccgtga ggaactgccg
      241 tttcaagtcg aggaatggac cgtatatgaa acaggtcccc agccggattc ggtcacttcg
      301 atcattgatt tgctgaacac ggaggaaagc ataactgttt tattcgccag tccatcagct
      361 gttcacgtct ttgaagaaaa agcggttccc gtgatcggtt ggagtggcta tacagtcggt
      421 gcgattggac atgtcactga aaaggcattg cttgaagctg gcgccaacgt ggacatcaag
      481 cctgaaatat atactcttaa ggaactcgtc aatgcattgg cgagacgaaa ggaagaaatc
      541 caatgactca acttcaattc agacgtaatc gccgtctgcg caactcggcg acattgcgct
      601 ctatggtaag ggaaacaatc ttgcagaagg aggatttcat ttatccgctt ttcatcattg
      661 aaggcgaaaa cgtgaaaaat gaaatcagct cgatgccggg catttatcaa ttgtcacttg
      721 atcgattgat ggaggaagtc gatgaagtcg tttcattagg catcccatcc gtcatcctct
      781 tcggcatccc agcagtgaaa gatgcgacag gtacaggtgc ttttgatgat gaagggattg
      841 tacagcgtgc gacgcgtctt atcaagggaa atcatcctga cctcgtcgta attgcggaca
      901 cttgcttatg tgagtatacc gaccatggtc actgcggagt tgtgcatgga tccgacgtat
      961 tgaatgatcc gtcccttgaa ctgttggcaa aaactgctgt cagccaagcg aaagcgggcg
     1021 cagacatcat tgctccatcc aatatgatgg acggctttgt cgtcgctatc agacaagcgc
     1081 ttgatgaaga gggctttgag aatgttccaa tcatgtccta cgcggtcaaa tatgcatctg
     1141 catactacgg accattccgc gatgctgcag aatcggctcc gcagtttgga gatcgcaaga
     1201 catatcagat ggatcctgcc aaccgcatgg aagcactccg cgaggcggag tcggatgtca
     1261 tggaaggtgc ggatttccta atcgtcaaac ctgcattgtc gtatttggat atcatgcggg
     1321 atgtgaagaa caatgtcctt cttcctgtag tcgcttacaa tgtgagcgga gaatattcga
     1381 tggtgaaggc tgcggcacaa aacggttgga tcaatgaaaa agaaatcgta ttggaaacgt
     1441 tgacaagtat gaaacgcgca ggtgcagatc tcatcatgac ataccatgca aaagacgcag
     1501 cgcgttggct ggagggaaaa taattgggta aaagctatga aaaatccaag caggcgttcg
     1561 cggaagcagt gaagttaatg ccaggtggcg tgaactctcc tgtccgtgct tttaaatcgg
     1621 tcaatatgga tccgattttc atggaaagcg gaaaagggcc aatcattaag gatattgatg
     1681 gcaatgaata tatcgactat gtcctatcat ggggtccact gattttaggg catgccgatc
     1741 cagatgtcgt ggaaggcatc aagaaagtgg cggaaacagg ttcgagcttc ggcgcgccta
     1801 ctttgcttga aaatgaactc gcgaaaatcg tcatcgaccg tgtaccttcg attgaggttg
     1861 tccgaatggt ttcgtctgga acagaggcta cgatgagcgc attgcgtctc gcgcgcggat
     1921 atacgggacg caataagatc ttgaagtttg aaggatgcta ccatggccac ggagattcat
     1981 tgcttattaa agcgggatct ggcgtcgcta cgctgggcct gccggatagc cccggagtac
     2041 ctgaaggtat tgcaaagaat acgattacag tggcgtacaa tgacatggat ggcgtccgtg
     2101 ccgcattcga acaattcggc gatgacattg ctggtgtaat tgtcgagccg gttgccggaa
     2161 acatgggagt tgtccctcca gagccaggtt tccttgaagg attgcgtgaa atcacagaga
     2221 agaacggcac tttgctcatt ttcgacgaag ttatgacggg cttccgtgtt gattacaatt
     2281 gcgcgcaagg ctatttcgga atcactcccg acctcacttg tcttggaaaa gtgatcggcg
     2341 gcggacttcc ggttggggca tatggcggaa agcgtgaaat catggaaaat atcgcacctg
     2401 caggaacggt ctaccaagca gggacattat ccggaaatcc aatggcgatg acagcaggca
     2461 ttgaaacatt gaaaaaattg acaccggctt cgtacgatca tttcaagaag cttggtgatc
     2521 agctggaggc tggcttccgt gaggcggcga cgaaatacaa tatcccgcat accgtgaacc
     2581 gtgccggctc catgatcggt ttcttcttta caaatgaaaa agtatacaat tatgaccaag
     2641 cgaagacgtc cgacctggaa ctgtttgcgg agtatttccg cttgatggca gaagaaggca
     2701 tcttcctgcc gccttcccag ttcgaaggaa tgttcctatc ctcttcacat acagaggagc
     2761 atattgaaca gactgtccaa gcattccatc gcgtctttga aaaattggca caataatatt
     2821 acaccaccca ttgtcatttc gttgacaatg ggtggttttt attctaccta ccccaaccga
     2881 tgcatataat ggcaagtgaa tgaaacaggg gagggaaacg gattgaaaaa attacaatgg
     2941 aatatggaag aggtctttta ttttccggaa aatgtcggag tacccaagga tgcgagacta
     3001 gttaaagtga aagcggggtt cacggagaag cggtcagaag acgcggtcag gctttccggc
     3061 atttatcaca ttgccgctaa agtggacttt gctgagggac aacggagtga agcgattccc
     3121 aatgatgcag tctatgtcga tgatgttgag attgaagggg atactggcta ttttgaatac
     3181 gccgtgccgt tatatatcga cttgcctcct gaagtcgagc atccattgca tatcgaagcg
     3241 acagacatca aacccacctt tgacggaca
//
LOCUS       DASULI010000211         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_819214, whole
            genome shotgun sequence.
ACCESSION   DASULI010000211 DASULI010000000
VERSION     DASULI010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819214"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1058)
                     /gene="fni"
                     /locus_tag="VFX34_04560"
     CDS             complement(24..1058)
                     /gene="fni"
                     /locus_tag="VFX34_04560"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX5564210.1"
                     /translation="MTGSIHQRKAEHIEITLNEKVTGNHITTGLERVHFIHNALPEID
                     FNEISIESKFVGQVNPTPFMISSMTGGAVFAETINRNLALAAEEKGWILALGSMRALI
                     ESQEHRASFQVRQYASSVPIIANLGAVQLNYGFGVEQCRQIIEMTDANALVLHLNSLQ
                     EVIQPNGDLNFKDLLVKIEELTKVLEVPVGIKEVGWGIDGMTAKKLTDVGVSFIDVAG
                     AGGTSWSQVEKYRSTDPVKQAAAETFSVWGIPTVDCVVSVREKIGEQSIVASGGMSTG
                     LDAAKTIALGADVVGFGRSILKEATQSPEDVMRVMETKELELRMAMFGIGASTLAELQ
                     ATDRVQYMKR"
     regulatory      1264..1411
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1471..2556
                     /gene="ribD"
                     /locus_tag="VFX34_04565"
     CDS             1471..2556
                     /gene="ribD"
                     /locus_tag="VFX34_04565"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012958396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5564211.1"
                     /translation="MDHADYMKLAIDLAKLAEGQTSPNPLVGAVCVKDGQIVGTGSHM
                     RAGTPHAEVHALNMAGSNAAGADLYVTLEPCAHVGKTPACTTAILSSGIRRVFIASND
                     PNPAVNGKGIRLLRDAGIEVITDLCKDEADFSNRAFFHFIQHKKPYVTLKAAATLDGR
                     LATQNGNSKWITSESSRQDVHTLRHTHDAILVGVNTILHDNPFLTTRLPHGGKNPIRI
                     ILDRHLRTPKTANVIKDKAAETIIFTLESNELHTFADFPLVQVESIPEHANFLDEVLT
                     RLAKKGIMTLLVEGGSQVHSSFIQSRLANELYLYMAPKLVGNGPSLFMDQSRSSIAES
                     EPLRILDVRRFGEDIRLHALFPIGGAS"
     gene            2557..3207
                     /gene="ribE"
                     /locus_tag="VFX34_04570"
     CDS             2557..3207
                     /gene="ribE"
                     /locus_tag="VFX34_04570"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017187126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5564212.1"
                     /translation="MFTGIVEELGTVHAIKKGSTSMQLTIRCRHVLSDVKKGDSISVN
                     GVCLTVSEFSKTEFIADVMPETFKATTLFTLKSGSSVNLERAMAANGRFGGHFVTGHV
                     DCIGEIVAVRPIENAVYMDIQMEPELHSHLVPKGSITVDGTSLTIFGISEQGFTISLI
                     PVTQADSVLGHKRVGDYVNVECDILAKYLERIFSRKQTEPTGGLTMDVLAKSGFLD"
     gene            3220..>3981
                     /gene="ribB"
                     /locus_tag="VFX34_04575"
     CDS             3220..>3981
                     /gene="ribB"
                     /locus_tag="VFX34_04575"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX5564213.1"
                     /translation="MFSTVEKAIEELKKGKAIIVVDDESRENEGDFVALADFATPGMI
                     NFMASEGRGLICVPIEQQLAEHLELAPMTNMNTDAHGTAFTVSIDHASCHTGISAFER
                     SETIMKMLQSNAKPSDFRRPGHIFPLVAKAGGVLERPGHTEAAVDLARLAGTTPAGVI
                     CEIMNGDGTMARGKELVEIAKQHELVILTIEQLIAYRKRNETLIERVVDIHLPTQYGD
                     FTAITYIEKLTGREHMALVKGRLKEIDSMGAPLVRI"
BASE COUNT         1078 a          961 c          908 g         1034 t
ORIGIN      
        1 ttgcaagtgg ccaactaaat cattcatctt ttcatatatt gaacccgatc ggtcgcttga
       61 agttcagcca acgtagaagc tccaatcccg aacatcgcca tacgcagctc caattctttc
      121 gtttccatca ctctcatgac atcttcggga gattgtgttg cttctttcaa tatggatctc
      181 ccgaagccga caacatcggc acccaatgca atcgttttcg cagcgtccag tccagtggac
      241 atgccaccac ttgcgacaat cgattgctcg ccgatctttt cacgtacaga cacgacacag
      301 tcaaccgttg gtattcccca tacgctgaac gtttcggcgg cagcctgttt aacagggtcg
      361 gtggaacggt atttttccac ttggctccaa gatgttccgc ctgccccagc tacatcgatg
      421 aaagaaacac cgacatccgt aagcttttta gccgtcatgc catcgattcc ccaaccgact
      481 tcctttatgc ctactggaac ttccaatact ttagtcagct cttcaatttt cactaataaa
      541 tccttgaaat tcaaatctcc atttggttga atgacttcct gaagactgtt caggtgaagt
      601 accaacgcat ttgcatccgt catctcgatg atttgccggc attgttcaac gccaaatccg
      661 taatttaatt gaacagcgcc caaattggcg ataatcggaa cggatgacgc gtattgccgc
      721 acttggaatg aagcccgatg ctcttggctt tctattaacg cccgcataga tcccaaagct
      781 aggatccaac ctttctcctc ggcagcgagt gctaaattac ggttgatcgt ttcggcgaat
      841 accgcccctc cagtcatgga actgatcatg aaaggtgttg ggttcacttg gccaacgaat
      901 ttactttcaa ttgatatttc attgaagtca atttcaggca aagcattatg aatgaaatga
      961 accctttcca gacctgtcgt aatatggttc cctgtcactt tttcattgag cgtgatttca
     1021 atatgctccg ctttacgttg atggatagaa ccagtcattt accaacatcc ctcttcccta
     1081 gcacgaagat acccttcacg cttcaatcct cgttcacgct ttttatttcc atatcaatta
     1141 taaattaacc aaattattat ttcagctatt tgtcatcata tcatgccttt aaaacattta
     1201 aaataaatca gtaacttggc aaccattccc gaagtgtgtt aaatttgaat cggtcaattc
     1261 aaaacgactc ttcggggcaa ggtgaaattc cttaccggcg gtgatcaagc gcatagcttg
     1321 tcagtccgtg acccgttctt ccctatggaa gacggtggag ctggtgtaac tccggcaccg
     1381 acagttaaag tctggatggg agaagttgtt cgtatttgat ggccatgccc aactatatcc
     1441 gccctttcat gaaaagaaag ggggaattta atggaccacg ccgattatat gaagttagcc
     1501 atagatcttg caaagcttgc ggaaggccag acttcaccga atccacttgt cggtgctgtc
     1561 tgtgttaaag acggccagat tgtcgggaca ggctcgcata tgcgggctgg aacgcctcat
     1621 gcagaagtgc acgccctcaa tatggccggg tcgaatgcgg ccggtgcaga tctatatgtc
     1681 actttggaac cgtgtgcaca tgtcgggaag acgccagcat gcactacagc aatcctatca
     1741 agcggcattc gccgtgtttt catcgcgtcc aatgatccga atcccgccgt aaatggaaaa
     1801 gggattaggc ttcttagaga cgccggaatt gaagtgatca ccgatttatg caaagacgag
     1861 gcggatttta gcaaccgggc attttttcat tttattcaac acaaaaaacc ctacgtcaca
     1921 ttaaaggcag ccgccactct cgacggccgt cttgcaacgc aaaatgggaa cagcaaatgg
     1981 attacgtctg aaagttcaag acaagacgtc cataccctcc gccataccca tgatgcaatt
     2041 ctcgtagggg tgaatacaat tcttcacgat aaccctttcc tcaccacccg tttgccccat
     2101 ggtggaaaga acccaatacg aattatttta gatcggcatc ttaggacgcc gaaaactgcg
     2161 aacgtcataa aggacaaagc agcggaaaca atcatcttca cgctcgaatc gaatgaattg
     2221 catacctttg ccgatttccc actcgttcaa gtggaatcga ttccggaaca tgccaacttt
     2281 ttggatgaag tcctcacgcg gcttgcaaaa aaaggcatca tgacgctatt agtggaaggc
     2341 ggaagccagg tccattcaag cttcattcaa tcacggcttg cgaatgagct gtatttgtat
     2401 atggcaccaa aattagtcgg aaacggccct tccctattca tggatcagag ccgatcatcc
     2461 attgcagaat cggagccgct ccgtatcctc gatgtcagac ggtttggaga agatatccgg
     2521 cttcatgcac ttttcccgat tggaggtgca agctgattgt ttacaggcat tgttgaagaa
     2581 ttaggaaccg ttcatgcgat aaaaaaaggt tctacttcga tgcagttgac cattagatgc
     2641 agacatgtgc tatctgacgt gaaaaaagga gatagcatat ccgtcaatgg cgtatgtctt
     2701 accgtatctg agttttcgaa aaccgaattc atagcggatg tcatgccgga aacattcaaa
     2761 gctacaacgc ttttcacatt aaagtccggc agcagtgtga acctggagcg cgcaatggcc
     2821 gcgaacggcc gatttggagg ccattttgtg acgggtcatg tcgattgcat cggtgaaatt
     2881 gttgcagtcc gaccgattga aaatgcagtc tatatggaca tccaaatgga accggaactt
     2941 cattcgcatc tagttccgaa aggttccata acagttgacg gcacttcttt gaccatattc
     3001 ggcatctcgg aacaagggtt tacgatatcc cttattcccg taacgcaagc cgattccgtt
     3061 cttggacata agcgtgtcgg agactacgtc aatgtggaat gcgatatact tgcgaaatat
     3121 ttggagcgaa tcttctccag gaaacaaacc gaaccgaccg gcgggttgac gatggatgta
     3181 cttgccaaaa gcggattttt ggattgagag gtgttggaat tgtttagtac ggtagaaaaa
     3241 gcgatcgagg aattgaaaaa agggaaagcg atcatcgttg tggatgatga aagccgtgaa
     3301 aacgaaggtg atttcgtagc gcttgccgat tttgcgacgc ctgggatgat caatttcatg
     3361 gcttcggaag gtcgcggact catatgcgta ccgattgaac aacagctggc ggaacatctg
     3421 gaattggcgc ctatgacgaa tatgaatacc gatgcccatg ggactgcatt cacagtcagc
     3481 attgatcatg catcttgcca tacgggtatt tctgcatttg aacgttccga aacgatcatg
     3541 aaaatgcttc aatcaaatgc gaagccttcc gatttcagaa gaccggggca cattttcccg
     3601 ttagtggcga aagccggcgg tgtattggag cgcccgggac ataccgaagc agcggtcgac
     3661 cttgcccgtc ttgcaggaac aactccagcg ggcgtcatat gcgaaatcat gaatggcgac
     3721 ggcacaatgg cccgtggaaa agaactcgtg gaaatcgcca agcaacatga gctcgtcatt
     3781 ttgacgattg aacaattgat tgcatacagg aagcgtaatg aaacactcat tgaaagggtc
     3841 gtcgatattc acttgcctac tcaatatggt gatttcaccg ctatcacata tatcgaaaag
     3901 ttgacaggcc gggagcatat ggctttagtg aaaggccggc tgaaggagat cgattccatg
     3961 ggtgccccac tcgtccgaat a
//
LOCUS       DASULI010000212         4194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_241018, whole
            genome shotgun sequence.
ACCESSION   DASULI010000212 DASULI010000000
VERSION     DASULI010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241018"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="VFX34_04580"
     CDS             <1..1136
                     /locus_tag="VFX34_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5564214.1"
                     /translation="KVHQMLALRERDYINGKMKGHLSHISYFLSGATRKAYWEGHVEL
                     IPNVFQEMFRLLQKTTNKPMVMAVASPMDEHGYFSLGTNADYAVDFIGDVPFVLEVNK
                     HMPRTFGANQIHISQIAGFIENDLPLTEEVAPVITEKDQKIAEYITEDIQDGDTLQIG
                     IGAIPNAVMKMLKDRRHLGIHTEMLTDGIVDLVEAGAVDGMKKSSHKGKIIATFAFGS
                     QRLYDFLNNNPSVEFQPVSVVNDAYEIAKEDHMVSINATTEVDLYGQCASETVAGRYY
                     SSTGGQADFARGVRLSKYGKGYVCMHSTAKNDTISRIKLQLAPGSVVTTSKNYVDNIV
                     TEYGIARLHGKSLSERAKALISIAHPKFREELMQEAKEFGLIE"
     gene            1594..1947
                     /locus_tag="VFX34_04585"
     CDS             1594..1947
                     /locus_tag="VFX34_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5564215.1"
                     /translation="MEWIIILAIVAFFAWRMMPAKGVKSISTDELKPMLKDKSKQFID
                     VRTPAEYKGRHIKEFENIPLNLLKSKLDKLDKNKEIVVICQSGMRSSQAAGILKKSGF
                     TGVTNVRGGMSAWLG"
     gene            2139..2960
                     /locus_tag="VFX34_04590"
     CDS             2139..2960
                     /locus_tag="VFX34_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5564216.1"
                     /translation="MNKDKATIGRDPYEIEQREWKQRQSKERYKQLLTIASPIFLLLL
                     WEVMSRTGIMDIRFFPPPSAILGTFGKMIASGAMADHIGVSLYRIFAGFLAGVIPGVV
                     IGLLMGLYSPIRHFVQPIVMALMPIPTLALLPIIIILFGIGDLSKVVTIAGSVFFPVV
                     INTAAGVMNIDRIYLDVADNYGAGKMQFFFKIALPGALPVMLEGIQMGQAIALLTIVA
                     AEMMGATSGIGYLIWTSYKAFLLKEMYVGLILISFFGYLFSLMLRGLQKKLLPWR"
     gene            2965..3753
                     /locus_tag="VFX34_04595"
     CDS             2965..3753
                     /locus_tag="VFX34_04595"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564217.1"
                     /translation="MRKKAKIEIRNLTKAFYKKQSSVTALDDISLSIGEGEFVCIVGP
                     SGCGKTTLLRILAGLEQPSVGEFEIRSEQEGRPLQSMVFQERGVIPWLTVKENVAFGL
                     KMRKLPKEVIRERTDYYLQKTGLDRFSNLYPKELSGGMKQRVSIARAFANDPEILLMD
                     EPFAALDEQNKFILQEELLSIWSETKKTVIFITHSIDEALLLSDRILLMSAQPGKIIQ
                     QIRIDTPRPRTMEDIRKDSTLSEQFVDIWKHLQDEVQRSRKENR"
     gene            3750..>4194
                     /locus_tag="VFX34_04600"
     CDS             3750..>4194
                     /locus_tag="VFX34_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5564218.1"
                     /translation="MKWGEKVKGFKYGWMMILAAMLLLAGACAKKEPAPQTKVETPPG
                     DTVIDPPSGDLAPLDKRVKVLIAEDGAASGAGFYIAKEKGYFEDYNIEVEFADFANSD
                     DMLPALAAGEVDIAGGVSTASFFNAIAQGIDVRIIADKGHNMPGKS"
BASE COUNT         1149 a          862 c         1131 g         1052 t
ORIGIN      
        1 ttaaagtgca tcaaatgttg gcattgcgtg aacgtgatta tataaatggt aagatgaaag
       61 gccatctttc tcatatttcg tacttcctga gtggagcgac gaggaaggca tattgggaag
      121 ggcatgttga attaattccc aatgtattcc aggaaatgtt tcgattgctg caaaagacga
      181 cgaacaaacc gatggtgatg gcagtcgcgt cgccgatgga cgaacatggc tacttttcgc
      241 ttggaacgaa tgcggattac gctgtggatt ttatcgggga cgttcctttt gttttggaag
      301 ttaataagca tatgccacgc actttcgggg cgaatcaaat ccatattagc caaattgccg
      361 ggttcattga aaatgatttg ccattgactg aagaggtggc gccggtcatt acggagaagg
      421 atcagaaaat tgcggagtat atcacggagg atatccaaga tggcgacacg ctccagatcg
      481 gcatcggggc cattccgaat gctgtcatga agatgctcaa agaccgcaga catttaggca
      541 tccatactga aatgctgacg gacggcatcg tcgatcttgt tgaagcgggc gctgtggatg
      601 gaatgaaaaa gtcgtcccat aaagggaaga tcattgcgac attcgctttc ggttcccaac
      661 gcttgtatga tttcctgaac aacaatcctt ccgtggagtt ccagccggta agcgtcgtca
      721 atgatgcata cgaaattgca aaagaggatc atatggtgtc gatcaatgcg acgaccgaag
      781 tagatttata tggccaatgt gcatcggaaa ccgtggcggg acgttattac tcgtctacag
      841 gcggccaggc cgattttgct agaggcgtca ggctgtcgaa atacggcaaa gggtatgtat
      901 gcatgcattc gacggcgaaa aatgacacga tttctagaat caaactgcag ttggcacctg
      961 gttccgttgt aacgacatca aaaaactatg tcgataacat cgtaacggag tatggaatcg
     1021 ccagattgca cggcaaatct ttgtccgagc gtgcaaaagc attgatcagc atcgcgcacc
     1081 cgaagttcag ggaagaatta atgcaggaag caaaagaatt tgggttgatt gagtagggag
     1141 gagcctatag ggcttctctt tttttgtgta gttgaccaat ttacagttga tttgtagtcg
     1201 aacggtgata aatttagtcg agtcggtggt aaagtcgtct gagtcggtgg taagacgtgg
     1261 agattcggtg gtaaagccgt ctgaatcggt ggtaaagtcg cctgaatcgg tggtaacacg
     1321 ccccgagtcg gtgataatgc ttgaaaagcc gttggaagtt gaagaaaagc cccactcggc
     1381 gagggaaata ttggccaagt ggtgataaac tccgtctgag tggtgataaa ctccagttaa
     1441 ccggtgataa agtccatcta agcggtgata actacttcct aagcggtgat aaacataatt
     1501 gttgcctcct ttatacccat ggtggtatag tgaattcata gtttaaatac tgcgtggggt
     1561 atattaagtg acgaccctaa ggagggaaaa ccaatggaat ggatcatcat cctagcaatc
     1621 gtcgcatttt tcgcatggcg catgatgccg gcaaaaggcg taaaatcgat atcaaccgat
     1681 gaattgaagc caatgttgaa agataagtcg aagcaattca tcgacgtccg cacgcctgcg
     1741 gaatacaaag ggcgccatat caaggaattc gaaaatattc cattgaactt gttaaagtcc
     1801 aagttggaca aactcgataa gaataaggaa atcgtcgtca tttgtcaaag cggcatgcga
     1861 agttcacaag cagcgggcat cctgaagaag tcgggtttca caggtgttac taatgttagg
     1921 ggcggcatga gcgcatggct cggataagga ataaaacact aaagcatctg ctgcggaatt
     1981 cgcggcaggt gcttttttgc gtccaaagtc aatagccccg catccaaccc cattcccatc
     2041 cacgcacaca aacctcgccc attcgctaaa aaccgcgcca tttgcccaac caaacccata
     2101 ggcgtacaca tattattgtt atgaagaggt ggtgaacgtt gaataaagac aaagcgacta
     2161 ttggacgtga tccgtatgag atcgaacagc gtgaatggaa gcagagacaa tcgaaagaac
     2221 gctataagca gctattgacg atcgcttcgc caatcttcct tttgctgtta tgggaagtga
     2281 tgtcgaggac gggaatcatg gatatccggt ttttcccacc gccttccgcc attttaggga
     2341 catttggcaa aatgattgcg agcggggcga tggcagacca tatcggggtt tctttgtacc
     2401 ggatttttgc agggtttctc gcaggtgtca tacctggggt ggttatcggt ctgctcatgg
     2461 ggctttattc gccgatccgg cactttgtcc agcccattgt catggcgcta atgccgattc
     2521 cgacgcttgc attgcttccg atcatcatca tcctgtttgg aatcggagat ctgtcgaaag
     2581 ttgttacgat tgcagggagc gtgttttttc cagtcgtcat caatacggcg gcgggtgtca
     2641 tgaacatcga tcggatctat ttggatgtcg cggataatta cggagccggt aaaatgcagt
     2701 tctttttcaa aattgctttg cctggcgcat tgccggtcat gttggaaggg attcaaatgg
     2761 gccaggcgat tgctttgctc acgattgtcg ctgcagaaat gatgggggcg acttcaggga
     2821 tcggatattt aatttggacg tcgtataagg cattccttct gaaggaaatg tacgtcggac
     2881 tcatcctcat ttcatttttc ggctatctct tctccttgat gcttcgcggt cttcagaaaa
     2941 agttgttacc ttggaggtga ttggatgaga aagaaggcga aaatcgaaat taggaatttg
     3001 acgaaagctt tttacaagaa acaatcgagc gtgacagctc tcgacgatat ttccctctca
     3061 atcggagaag gggaatttgt ctgcattgtc ggaccgagcg gctgtggcaa aacaacatta
     3121 ctgcggatct tggcaggact tgaacagccg agtgtaggtg aattcgaaat ccgttcggag
     3181 caggaagggc gccctctcca atcgatggtc ttccaggaaa ggggcgtcat cccgtggctc
     3241 actgtaaagg agaatgtcgc tttcgggtta aaaatgcgaa aattgccgaa ggaagtcatt
     3301 cgtgagagga cggactatta cttacaaaag acgggacttg accggttttc aaatctctac
     3361 ccgaaggaac tgtccggggg gatgaagcag cgggtgagca ttgcccgtgc gttcgcgaat
     3421 gacccggaaa tcctacttat ggatgaacca ttcgctgcat tggatgaaca gaataaattc
     3481 atccttcagg aagaattgct ctccatatgg tcggaaacga aaaagacggt gattttcatt
     3541 acacatagca tcgatgaagc attattgctg agcgatcgga tcttgctgat gagcgcacaa
     3601 cctggcaaga tcattcaaca aatcaggatc gacacgccgc ggccgaggac gatggaagat
     3661 atccggaaag attcaacgct ctcggagcaa tttgtcgaca tttggaaaca cttgcaagat
     3721 gaagtgcaaa ggtccaggaa agagaataga tgaaatgggg agaaaaggtg aaagggttca
     3781 aatatggttg gatgatgatt ttggctgcca tgcttttact tgccggagca tgcgccaaaa
     3841 aagagcccgc tccgcagacg aaagtggaaa caccgcctgg cgatacagtc attgatccgc
     3901 catccggaga tttagcgccg ctggataaac gagtgaaggt gttgattgcg gaagacggtg
     3961 cggcttccgg ggcagggttc tatatcgcaa aggaaaaagg gtattttgag gattacaata
     4021 ttgaagtgga atttgctgat ttcgctaata gtgatgacat gctgccggca cttgctgctg
     4081 gggaagtcga tattgcgggc ggcgtctcga ctgcatcatt cttcaacgct attgcgcaag
     4141 gcatcgacgt ccggatcatt gcggataagg ggcataatat gcctgggaaa tcgt
//
LOCUS       DASULI010000213         4251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_117672, whole
            genome shotgun sequence.
ACCESSION   DASULI010000213 DASULI010000000
VERSION     DASULI010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117672"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..645)
                     /gene="spoVT"
                     /locus_tag="VFX34_04605"
     CDS             complement(109..645)
                     /gene="spoVT"
                     /locus_tag="VFX34_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HEX5564219.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDRDGEV
                     ILKKYSPISELGEFAKEYAETLYETLGIPALISDRDEMIAVAGLSKKDYLNRQLTREV
                     EEMLSSRKMVTEKLEKSVEWVPGQVEQVKSYCIAPIIAGGDTIGGVYLLSKVHFIGDV
                     EQKAAETAAHFLAKQMEQ"
     gene            complement(749..>4251)
                     /gene="mfd"
                     /locus_tag="VFX34_04610"
     CDS             complement(749..>4251)
                     /gene="mfd"
                     /locus_tag="VFX34_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014822296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5564220.1"
                     /translation="FLREKEIGTIVEELENGKDRQLIAGLSGGAKPIYIHALKEMLHK
                     PMLIVSPNLLQAQRIYEDLARLVGEENVHLYPAEELIAADFSFSSFDLRAQRIETLDH
                     MGRIGKGIYITPIAGMRKFLPRKERFFSSCLETAVGDELSIDDVLNKLVQMGYSRQQM
                     VTAPGEFAHRGGILDVYPLYMENPVRIELFDTDVDSIRTFSAEDQRSISKLETVRILP
                     STEFVFERLQYYELAEQLEKSLGKSLKKMTNPEAKENMTLHIQGDIELMRTGEPPEQI
                     GKYVSLLPEAPSFLGDYFPSDGIVLFDEIGRVLEVIQSLEKEEHEWFVSLLEEGKIVH
                     DAKLSFTFTEFHSMLEQAKVYFSLFIRSIPGIQVKRTLSISCKPMQHFHGQMNLLKNE
                     MERWQHTGFTIFIVADGKERMMKVQSVLADYGMETELSGKSSKSGHITVIDGDLSAGF
                     EFPLQRLAVVTDAELFKQKTKKKARPQKMTNAERIKSYSEIKPGDYIVHVHHGIGKYI
                     GLETLEVNGTHKDYLHIRYRADDKLFVPADQIDLIQKYVPSGEKEPKLHKLGGTEWKK
                     TKTKVSAAVKDIADDLIKLYAKREAEKGFAFSQDDDMQRAFENAFPYDETEDQLKSIE
                     EVKKDMERDRPMDRLLCGDVGYGKTEVAIRAAFKAVGDGKQVAFLVPTTILAQQHYET
                     MKERFSGFPVEVALMNRFRTKKEQDETLKGLKNGTVDVVVGTHRLLSKDVVYKDLGLL
                     IVDEEQRFGVTHKEKIKQLRTNVDVLTLTATPIPRTLHMSMIGVRDLSVIETPPANRF
                     PVQTYVMEHNYALIREAIERELSRGGQVFYLYNRVEDMTRKVEEIKDLVPDARVGYAH
                     GKMTERELESVILSFLEGEYDVLVTTTIIETGIDIPNVNTLLVHDADRMGLSQLYQLR
                     GRVGRSNRVAYAYFLYQRDKVLTDVAEKRLQAIKEFTELGSGFKIAMRDLSIRGAGNL
                     LGSQQHGFIDSVGFDLYSQMLQEAIDERRNGTSKEDVPDLEISLPIDAYIPDEYVKDG
                     YQKIQMYKRVKAIDSEEDYNELIDEMTDRFGDMPLETDLLLRIARIKVWGRQAGVESI
                     KKQRNIIEVRLSEEGTAKTDGGKLVSESMQFGRAVGFSMDGKQLVLTIDERHTGKRTS
                     FDILEAMMKLLPESGKEATA"
BASE COUNT         1034 a         1119 c          840 g         1258 t
ORIGIN      
        1 atgccccagg attccaacgc tacgcccttt atgcggtgta ttccggcgaa ttataaaaaa
       61 ccgtcccgca gctcaaattt cgcttcagga cggttttttc agtgctgatc attgttccat
      121 ttgttttgcg aggaaatggg cggctgtttc agcggccttt tgctcgacgt caccaataaa
      181 gtggactttg gagagtaaat ataccccgcc aatcgtatct ccgcctgcaa taatcggggc
      241 gatacaatag gatttcactt gctctacttg tccaggtacc cattcaacgg acttctccag
      301 cttttcggta accatttttc gactcgataa catttcttcc acttctcgtg tcagctgccg
      361 attgagataa tccttctttg aaagacctgc aacagcaatc atttcatctc gatcactgat
      421 caatgcaggt attccaagag tttcgtacaa ggtttcggcg tattctttcg caaattcccc
      481 gagttcggat atcggagagt atttcttcag aatcacttca ccatcgcgat ccgtaaaaat
      541 ttccaaaggg tccccttctc gaatgcgaag tgtcctacgg atttctttcg gaatgacgac
      601 tcttccaaga tcatcgattc tgcggactat tccggttgcc ttcattcttg atgcctcact
      661 ttcgccaaag ttaaaactct gtttcatttc agccctagta tgcatcgttt ttggacaaaa
      721 tatgcaacca gtccagatag tcggtatgtt atgcagttgc ttccttacct gattcaggca
      781 acagcttcat catcgcttcc aatatatcga atgacgttcg ttttcccgta tgtcgttcat
      841 ctatagtgag aacgagctgt ttgccatcca ttgaaaatcc aactgctcgg ccgaactgca
      901 tggattccga cacgagtttt cctccgtcgg tttttgctgt tccttcttcg gacaatcgaa
      961 cttctatgat attgcgttgc ttcttaatcg actcgacgcc tgcttgacgg ccccacacct
     1021 ttattctcgc aatccgaagc aataaatccg tctccagcgg catgtcgccg aatcggtctg
     1081 tcatttcgtc aatcagttcg ttgtaatctt cttcactatc aattgctttt acccgtttgt
     1141 acatctggat tttttgatag ccatccttaa cgtattcatc cgggatatat gcatcaatcg
     1201 gcaacgagat ttccaaatca ggtacgtcct ctttactagt gccgttccta cgttcgtcaa
     1261 ttgcttcctg gagcatttga gaatataggt caaacccgac agagtcaata aatccatgct
     1321 gctgggaacc caacaagttt cctgcgcctc ggatcgacaa atcccgcatg gcaatcttga
     1381 acccggagcc aagctcggta aattctttta tcgcttgcaa ccgcttctct gcgacatctg
     1441 tgagcacctt gtcgcgctga taaaggaaat acgcatatgc cacacgattc gagcggccga
     1501 ctcgtccacg aagctggtag agctgggaca agcccatccg atctgcatca tggacaagta
     1561 atgtatttac attcggaata tcaattcctg tttcaatgat tgtcgttgtc acaaggacat
     1621 cgtactcgcc ttccaaaaag ctgaggataa ccgattcaag ctcgcgttct gtcattttac
     1681 catgtgcata gccgactctc gcatccggca caaggtcttt gatttcttcg accttccgtg
     1741 tcatgtcttc cacccggtta tagagataga agacttggcc accacggctc aattcacgtt
     1801 caatcgcttc ccgaatcaac gcatagttat gctccatgac atacgtttga accgggaacc
     1861 ggttggccgg cggcgtttca attaccgata agtcccgtac gccaatcatg gacatatgaa
     1921 gggttcgcgg aatcggtgtt gccgttaacg tcagaacatc aacgtttgtc cgcagctgtt
     1981 taattttctc cttgtgcgta acaccaaaac gctgctcctc gtcgacaatt aaaagaccta
     2041 aatctttata aacaacgtct ttggataaca gtcggtgcgt tccgacgaca acatccaccg
     2101 ttccattctt taatcctttt aacgtctcgt cctgctcttt tttcgtacgg aagcggttca
     2161 tgagtgccac ttcgactgga aaccctgaaa aacgttcttt catcgtttca tagtgctgtt
     2221 gcgcaaggat tgttgttggg actagaaacg cgacttgctt gccatcgcca accgctttga
     2281 acgccgcccg aatggcaact tccgtttttc catagcccac atcgccacac agaagacgat
     2341 ccatcggccg gtctcgttcc atgtctttct taacttcttc aattgatttc aactgatctt
     2401 cggtttcgtc gtacggaaat gcattttcga acgcgcgttg catatcatcg tcctgcgaga
     2461 aagcaaagcc cttttcggct tcccgctttg catacaactt gattaaatca tctgcaatat
     2521 ccttaactgc cgccgatacc tttgttttcg tctttttcca ttccgttccg cctagcttgt
     2581 gcaacttcgg ctctttttca cctgacggaa catatttttg aatcaaatcg atttggtcag
     2641 ccggaacaaa cagtttatcg tccgcgcggt agcggatatg aagataatcc ttatgggtac
     2701 cgtttacctc aagcgtctcc aaaccgatgt attttccgat tccatgatga acgtggacga
     2761 tgtaatcgcc cggtttgatt tcagaataac ttttaatccg ctccgcatta gtcatctttt
     2821 gcggtcgtgc ttttttcttc gttttctgtt taaacaattc cgcatctgtt acaacggcga
     2881 gtcgttgaag tggaaactca aaaccggcag acagatcacc gtcaattact gtaatgtggc
     2941 cactcttgga actcttaccg gacagttccg tttccatccc ataatccgct aagacagact
     3001 ggaccttcat cattcgctct ttgccgtccg caacaatgaa tatcgtaaag ccagtatgtt
     3061 gccagcgttc catttcattt tttaataggt tcatctgacc atggaaatgc tgcatcggct
     3121 tacacgagat cgagagagtt ctcttcactt gaatgcctgg aatggagcgt atgaataagg
     3181 agaaatacac ctttgcctgc tccagcatgg aatgaaactc tgtaaacgta aacgaaagct
     3241 ttgcatcgtg gacaatcttc ccttcctcaa gcagggatac aaaccactca tgttcttcct
     3301 tttcgagtga ttgaatcact tccaggacac ggccgatctc atcaaataac acaattccgt
     3361 cagacgggaa ataatccccg aggaaagaag gtgcttccgg taacaatgaa acatactttc
     3421 cgatttgctc cggcggttct ccggttcgca tcagctcgat gtctccctga atgtggagcg
     3481 tcatgttctc ctttgcttcc ggattcgtca tcttttttaa actttttccg agcgatttct
     3541 caagttgttc tgcaagctca tagtactgca atcgttcgaa gacaaactcc gttgaaggca
     3601 aaattcgcac agtttcaagt ttcgaaatgg aacgttggtc ttctgccgag aacgttcgga
     3661 tcgagtcaac gtccgtatca aacaactcaa tacggacggg attttccata tataacggat
     3721 acacgtccaa aataccgcca cgatgcgcaa attcaccagg agcggtcacc atttgttgac
     3781 gagaatatcc catttgaact aatttgttca gcacgtcatc aatcgaaagc tcatccccca
     3841 ccgcagtttc cagacagctt gaaaaaaacc gctctttccg cggcaagaat ttccgcattc
     3901 cagcgattgg tgtaatatag attcctttcc cgattcgccc catatgatcc agtgtttcaa
     3961 tccgctgcgc ccgaaggtca aagcttgaga aggaaaagtc agctgctatc aattcctcag
     4021 ccgggtacag atgaacattc tcttctccta ccaacctcgc cagatcctcg tatatgcgct
     4081 gtgcttgtaa tagattcggt gaaacaatca acatgggttt gtgcagcatc tcttttaatg
     4141 catgaatata aatcggtttt gcgccgccgg aaagtcctgc aattagctgc cgatccttcc
     4201 cattctccaa ttcttccact attgtcccaa tctctttttc ccgtagaaat a
//
LOCUS       DASULI010000214         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_312113, whole
            genome shotgun sequence.
ACCESSION   DASULI010000214 DASULI010000000
VERSION     DASULI010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312113"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFX34_04615"
     CDS             complement(<1..206)
                     /locus_tag="VFX34_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564221.1"
                     /translation="MRVVIGGAHNGKREYVKRILKDEPNQWVDCSVGDLEIPTGCKVV
                     VDRIEHWLARAELPEAEAVEFIMK"
     gene            complement(158..751)
                     /locus_tag="VFX34_04620"
     CDS             complement(158..751)
                     /locus_tag="VFX34_04620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5564222.1"
                     /translation="MAGDHHLYLIRHLPTVGNRERKYIGWTDEQIGPVEETRRFKKVD
                     SRIVYGSDLLRARQTAANLMPDADYVADARLRECHFGGFEGKTYADLEMNKDYRSWID
                     DPYSFAPPGGESLVDVEKRVMEALVSLPDGAVVITHGGPIRIALTRFSPEPQDFWSWQ
                     IPHGSIWKLEWRNHDELKEGGRCVSLSEVPITGKGSM"
     gene            complement(724..1488)
                     /gene="cobS"
                     /locus_tag="VFX34_04625"
     CDS             complement(724..1488)
                     /gene="cobS"
                     /locus_tag="VFX34_04625"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX5564223.1"
                     /translation="MNGFMLALQFFTSIPIRKELPMERKDVTAMYISLPIVGGLIGIA
                     MYGVAVLFTDVIGTGSLLASVFVVVAGIALTGGLHLDGFADMGDAFFSYRDKEKRLEI
                     LDDPRIGAFGAMALFLLIIVKIALLEELIHRSEGLLPYFIAVPLLARAGMNVYFSTTR
                     LAKEKGIAHFFKGKLATMELMVWSVIKAIAVAVVLGFVLHSIIVPVVLLIVVCAAVFL
                     FRSWSMKHFGGVSGDLCGAFIEGTEALLWLAIIICI"
     gene            complement(1485..>2126)
                     /locus_tag="VFX34_04630"
     CDS             complement(1485..>2126)
                     /locus_tag="VFX34_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5564224.1"
                     /translation="QAEAARTKPVIEMNPILLKPLGNMKADVRFIGEKFEAVDGMEYR
                     KQFFDRALKAIRTSLDRLAESFDVLVIEGAGSPAEVNLNDREIVNMRIADMADVPVLL
                     VADIDRGGAIASIVGTLQLLSPEHRARVKGIIINKFHGDAALFQEGVDFIESYTGIRV
                     AGVIPHKMNHGIEEEDMDRPLTVAPAGTDIYAAWAEHVNAHIDWPFVLGLVEE"
BASE COUNT          522 a          669 c          459 g          476 t
ORIGIN      
        1 tctttcatga tgaattctac agcttccgct tccggcagtt ccgcccgtgc gagccaatgc
       61 tcgatgcggt caacgactac tttgcacccg gttggaatct ccaaatcgcc gacggaacaa
      121 tcgacccatt gattcggttc atccttgagg atccttttca catactccct tttcccgtta
      181 tgggcacctc cgataacgac acgcatcgtc cgccctcctt caattcgtca tgattgcgcc
      241 attcgagctt ccaaatcgag ccgtgtggaa tttgccatga ccagaaatcc tgcggttccg
      301 gcgaaaaccg ggtgagcgcg atgcggatcg gtccgccatg tgtgatgacg accgcgccat
      361 ccggcaatga aaccaatgct tccatcacac gcttttccac gtcaaccaaa ctttccccgc
      421 caggcggtgc gaaggaatac ggatcatcaa tccaacttcg gtaatcctta ttcatttcaa
      481 ggtccgcata cgttttccct tcaaatccac cgaaatggca ttcacgcaat cttgcatccg
      541 ccacatagtc tgcatcaggc atcaagttcg ccgcggtttg tctcgccctc agcagatcac
      601 tcccatacac gattcgactg tccacttttt tgaacctgcg ggtctcttcc accggcccga
      661 tctgctcatc cgtccacccg atatatttcc tctcgcgatt tccaacggtc ggcaaatgac
      721 ggatcaaata caaatgatga tcgccagcca taacaacgcc tccgtacctt cgatgaacgc
      781 cccgcataag tcacccgaaa cgcccccgaa atgtttcatt gaccagcttc tgaaaaggaa
      841 tacagctgca catacgacaa tgaggagaac gaccggcacg atgatggaat gaagaacgaa
      901 gccaagcacc acggcaacag cgattgcctt aataacagac cacaccatca actccattgt
      961 cgccagcttc cctttgaaga aatgggcgat ccccttctct ttcgcaaggc gcgtcgttga
     1021 aaagtaaacg ttcatgcctg cccgtgcgag cagcgggacg gcaataaagt acggcagcag
     1081 cccctcgctt cgatgaatca attcttccag caatgcgatt ttcacaatga tcaacaggaa
     1141 aagcgccatc gccccgaacg caccgatacg cggatcgtca agaatttcaa gccgcttttc
     1201 cttgtcgcga tacgaaaaaa acgcgtctcc catatccgcg aagccatcca gatgcaaacc
     1261 gcccgtcaat gcgatgcccg ccacaacgac gaaaaccgac gcgagcagcg agcccgttcc
     1321 gataacatcc gtaaacaata ccgccacgcc gtacatcgca atgccaataa gtccgccaac
     1381 aatcggcagc gagatgtaca ttgccgtcac gtctttccgt tccatcggca gctctttacg
     1441 gattgggatg gatgtgaaaa attgaagtgc aagcataaaa ccattcattc ttccaccagc
     1501 cctaggacaa atggccaatc gatatgcgca ttcacatgct ccgcccaagc ggcgtaaata
     1561 tccgttccgg ccggcgccac tgtcagtggc cggtccatat cctcttcttc gatcccatga
     1621 ttcatcttat gcggaatgac ccccgccacg cgtatgcccg tgtacgactc gataaagtcg
     1681 acgccttcct ggaataaggc ggcgtctcca tggaatttat tgatgatgat ccctttcacg
     1741 cgcgcccgat gttcaggcga cagcaattga agcgtcccga caatcgaagc gatggcgccg
     1801 ccccggtcga tgtcagcgac aagcagcaca ggcacatccg ccatgtccgc tatccgcata
     1861 ttgacaattt ccctgtcgtt caaattcact tccgcaggac ttccagctcc ttcaatgacg
     1921 agtacatcaa acgattctgc gaggcggtca agcgacgtcc ggattgcttt cagggcacgg
     1981 tcgaaaaact gcttccgata ttccatcccg tccaccgctt cgaatttctc tccgataaac
     2041 cggacgtcag ccttcatatt gccaagtggc ttcaacagaa tcgggttcat ttcaatgacc
     2101 ggctttgtcc gcgcggcttc cgcctg
//
LOCUS       DASULI010000215         7891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_327645, whole
            genome shotgun sequence.
ACCESSION   DASULI010000215 DASULI010000000
VERSION     DASULI010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327645"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1340
                     /locus_tag="VFX34_04635"
     CDS             159..1340
                     /locus_tag="VFX34_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016126542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX5564225.1"
                     /translation="MKFLLFIAALLVIFFLAFIVSNGKRQIKYKSVITMVVLQLVLAF
                     ILLNTEFGLVLIKGVANVFDKLLAYAAEGINFVFGGLANEGSFPFFMNVLLPIVFISV
                     LIGIAQYIGLLPIIIRFLGVVLSKVNGLGKLESFNAVASAIFGQSEVFISIKKQLPHI
                     PKHRMYTMCTSAMSTVSASILGAYMTMIEPRYVITALVLNLFGGFIIANIINPYKVSE
                     EEDIIDVPQERKQSFFEMLGEYIMDGFKVAVIVGAMLIGFVALIGMINSIFEAAVGVS
                     FSTALGYVFAPFAFIVGIPASEVVQAGTIMATKLLTNEFVAMMDLANIASSLSDRTVG
                     IISVFLVSFANFSSIGIIAGAVKGLHEEQGNTVARFGLKLLYGATLVSLLTAAVTGIM
                     L"
     gene            1697..2335
                     /locus_tag="VFX34_04640"
     CDS             1697..2335
                     /locus_tag="VFX34_04640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5564226.1"
                     /translation="MSFLKKTREFIDRHYKTPKGIIGMFIGEKMVRQHKTETNWSLEL
                     MNVQKGDRILELGCGAGFAIKLILEKDLAEEIVGLDNSPTIIRSAKIRNKKAINEKRA
                     KLVQADFNKLPFHNENFNTVFSIQTIYFWTDIAKTLSEIFRVLKPEGVVILTFSDGKE
                     DETWEEIRGITENKVIPYMKYAGFKDVSLFKGPNSRGYHTVAVRGTKYNQTS"
     gene            2633..3655
                     /locus_tag="VFX34_04645"
     CDS             2633..3655
                     /locus_tag="VFX34_04645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5564227.1"
                     /translation="MQLINEVAKKFNGIPLSILDLAMINEGSNAGQSFKNSVDLAQHA
                     EKWGYNRFWLAEHHNMPGVASSATSVIIGHVAGATETIRVGAGGVMLPNHAPLVIAEQ
                     FGTLDALYPGRIDLGLGRAPGSDQATAYALRRTLQSSGNDFPEQLDELRAYFKHDPTA
                     RVRAFPGEGQDIPIWLLGSSDFSARLAAELGLPFSFASHFAPTYMMHALNLYHRNFKP
                     SKDLGKPYTMLGINIIAADTDEKAQWLATSHQQMFLNIRRGITTEFKPPIDDVDAVWN
                     DLEKSAVAELLEWPSTIVGSPETVKKKLEAFMDMTKADECIISSGIFNHEDRLRSYEI
                     VSEMMA"
     gene            complement(3716..4936)
                     /locus_tag="VFX34_04650"
     CDS             complement(3716..4936)
                     /locus_tag="VFX34_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX5564228.1"
                     /translation="MKNIFMAYYRASLILKISVALVLGIIAGFLLGEQAAHLAPLGDL
                     LLRLLTFLIIPLILFTLIVGVNQTNIANLGRMGSKVFIYYTVTSALAIIVGLLVASIL
                     RPGTGMHLTGNETFDVPENPGIIHVLLNIVPSNIATAFTEMNLLGIIFTALAFGIAIS
                     VMRSSEKHGGLGDSLLTNVTALNEASLIILKAILQYVPIGIFAIIANTVGSQGIDTLV
                     SLGGMIGVFYIALLVHIVLYIILLLLFGIKPGPFFKYARTPMITAFVTQSSTGTLPLT
                     LDAAKKMGIPKSLYGFSLPLGATVNMDGAAIRIAVSAVFAANVAGTPLTIPDMLMVVL
                     IGTLASIGTAGVPGAGIVMIATVFVQLGLPMEAVALLTAIDALVGMGATMLNITGDLV
                     GTKLIAQQEQRRKT"
     gene            5082..5450
                     /locus_tag="VFX34_04655"
     CDS             5082..5450
                     /locus_tag="VFX34_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564229.1"
                     /translation="MVQVQEQADTVERNEFREAPVTTVFKKFGNNKDVSLVFGDPIEV
                     GLTKVVPVAKLRYGFGGGGDGKGGDGGGGGFVVNPIGVYEITPDRVRFKPARSGGLVI
                     GLALVSGLFFFLGRKSCKKR"
     gene            complement(5495..6811)
                     /gene="brnQ"
                     /locus_tag="VFX34_04660"
     CDS             complement(5495..6811)
                     /gene="brnQ"
                     /locus_tag="VFX34_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEX5564230.1"
                     /translation="MNDFSNKETLKLGLLMFALFFGAGNMIFPPLVGQLAGGNVWIAI
                     AGFLITGVGLPFLGVVAVAMSGSQLRDLAGKAHPLFGLVFAIVVYLALGPFFAVPRTA
                     TVTFEMGLAPFVSDSAERMALLLFTVLFFAVTAWLSLKPNKLVDRFGNVLTPVLLALV
                     AFIVIAGIIKPDGTSGPPQEPYIQHPFIKGFIEGYLTMDAMAALVFGIVVINAIKARG
                     ISDRRVTLAITMKAGVFAVVGLCLVYVGLAYLGTTSRLVAPNAANGGEILTQLVYSLL
                     GTTGQVLLGTAVFLACMTTSVGLVSSCAAFFSGTFPKLTYRYVVLIVCVFSTFIANIG
                     LTHLISLTLPVLIGIYPLAIVLITISFFHNWFNGYREVYLFALAAAGFVGFFDMLKAF
                     GLPISPIVHVLRHLPLYEEGIGWIVPAIVFGFIGFVIGSIRKRVRP"
     gene            7090..>7891
                     /locus_tag="VFX34_04665"
     CDS             7090..>7891
                     /locus_tag="VFX34_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5564231.1"
                     /translation="MLNVTENRFANYPQQPHGGKLVDKVLTGTEREEELERARLMPSI
                     MVDLEAVITLEMIATGVLSPNEGFMNEVDYKSVLNEGRLSNGTVWPVPLSFAPIGNRN
                     KEIIKTLSVGDEVALSDEQNEPVAILKIEDIFDYDKDYRASHLFGTTDRNHPGVDAIF
                     RRMGDVALGGPITLLRRVDWGPFEKLRLEPKDTWHEFYEVKKFRSAAGFITGANPLHR
                     GHEYIHKNALEEIDGLLLQPLVEMAKREYTRHEFRMLSYRSVLDTYYPK"
BASE COUNT         2221 a         1701 c         2021 g         1948 t
ORIGIN      
        1 aagttggaaa aagacatatg acattttgct ttgtatataa acacctgatt tgtcgaataa
       61 ctacagtgat tacttaaaat tattttcggg aagatacaag aaaatggcat tcgttgctcc
      121 atactacagt tcatgcatct agagaggggg aaaccgcttt gaaattccta ttattcatcg
      181 cggcgttact tgttatcttt ttccttgcat tcatcgtgag caatggtaaa aggcaaatca
      241 aatataaatc ggttattacg atggtcgtct tacagcttgt gttggctttc atcctattga
      301 atactgaatt cggacttgtt ctcatcaagg gagtggcgaa tgtattcgat aagctattgg
      361 catatgccgc agaagggatc aactttgttt ttggcggtct tgccaatgaa ggttctttcc
      421 cctttttcat gaatgtatta ttgccgattg tattcatatc agtactcatc ggtatcgcgc
      481 agtacatagg gctccttccg atcatcatcc gttttctcgg agtcgtattg agcaaagtca
      541 atggtcttgg aaaattggag tcgttcaatg cggtcgcctc tgcgattttt ggacaatccg
      601 aggtgttcat ctccattaaa aaacagctgc cgcatattcc gaagcatcgg atgtatacga
      661 tgtgtacgtc ggcgatgtcg acggtatctg catcgattct aggtgcgtac atgacgatga
      721 tcgaaccgag atacgtcatt acggcacttg tcctgaacct tttcggcgga ttcatcatcg
      781 cgaatatcat caacccttac aaagtgtcgg aggaagaaga tattatcgac gtgccgcaag
      841 aaagaaagca gtccttcttt gaaatgttgg gcgagtacat tatggacggc tttaaagtgg
      901 cggtcattgt cggcgccatg ctgatcggtt ttgtcgcatt gatcggcatg attaacagca
      961 tatttgaggc ggcggtcggc gtcagcttct cgacggcgtt aggatatgtc tttgcgccat
     1021 ttgcattcat cgtcggcatt ccggcatcgg aagttgtaca agccggtacg atcatggcga
     1081 cgaaacttct gacgaacgag ttcgtggcga tgatggactt ggcgaatatc gcgtcatcat
     1141 tatcggacag gacagtcggc atcatttccg tattccttgt gtcatttgcc aatttctctt
     1201 caatcgggat cattgccgga gctgtcaaag gcttgcatga agaacaaggg aatacagtcg
     1261 ccagattcgg tttgaaattg ctttatggag cgacgttggt cagtttattg acagcagcgg
     1321 ttacaggcat catgttataa gggagtcccc aatccgtaac gggttcggtt gatggggaga
     1381 aaacaaaaaa aacacagcat tttgggacac aattcgcata tgaattggtg tccctatttt
     1441 gttgttgtat caacttttga tgttgctaat tggatatcgg aaagaaaaat atagaaggaa
     1501 ggtgagaaat ctatgctaaa ataaatcaaa gattgtgaaa taaggttatt caactgacgg
     1561 ggcaggattg tataataaga catataaaag gtataccact ttattaattt tgttaatgtg
     1621 aaagcccaga atatagaagg aacacacaaa ctttgtttaa ttataaggtc ttctacgatt
     1681 cgaagaggag atatgtatga gtttcttaaa aaaaactaga gaatttatcg accgtcatta
     1741 taaaacacca aaaggtataa taggtatgtt tattggtgaa aaaatggtaa gacagcacaa
     1801 gactgaaaca aattggtcgt tagagctaat gaatgttcaa aaaggagaca ggattttaga
     1861 gttggggtgt ggggcaggtt ttgcaattaa attaattttg gaaaaggatt tagccgaaga
     1921 aatagttggt ttggacaatt ctccaacaat tattcgttcg gcaaaaatta gaaacaaaaa
     1981 ggcaataaat gaaaagagag caaaactagt tcaagcagat ttcaataaat tgccctttca
     2041 taatgaaaat ttcaacactg tcttcagtat ccaaactatt tatttttgga ctgatatagc
     2101 taagacatta tcagaaatct ttagggttct aaagccagaa ggagttgtaa tccttacttt
     2161 ttctgacggg aaagaagatg aaacttggga agagataaga ggcattactg aaaataaagt
     2221 aattccgtat atgaagtatg caggatttaa ggacgtttct ttatttaaag gcccaaattc
     2281 aaggggatat cataccgttg cagtgagggg aactaaatat aatcaaactt cttagtcttc
     2341 agctaaaggg tgctttagat caataaggat aatagaatat tactttaaaa gcgctcagaa
     2401 atctgagcgc tttcacttgt ttttattagc tatttcactg ttgaaagagt tgctgattaa
     2461 cctgttaatt acattgttaa tcgtactttt cttgcctcaa tcgaacccgt tgccctttcc
     2521 ggggggctct tttttatgcg gttattccgt cagttccctt caatggaagt ggatgagccg
     2581 tagcggtatc atggaatagt aggaataacg ccgattaaaa ctattggagt gaatgcaatt
     2641 gataaatgag gtagcgaaga agttcaacgg catcccccta tcgatcctcg atcttgcgat
     2701 gatcaacgag ggaagtaatg ccggccaatc tttcaagaat agcgtcgatc ttgcccagca
     2761 tgccgaaaag tggggatata accgcttctg gcttgctgaa catcacaata tgcccggcgt
     2821 cgcaagttcg gcgacatctg tcatcatcgg gcatgtggcg ggtgcgacgg agacgatccg
     2881 tgtcggggct ggcggcgtca tgctgccgaa ccatgcgccg ctcgtcatcg cggagcagtt
     2941 cggaacgctc gacgcgttat atccgggcag aatcgatctg ggtcttggcc gcgcccccgg
     3001 cagcgaccag gcaacggcct atgcgctgag acggacattg caaagcagcg gcaatgattt
     3061 cccagagcag ctggacgagc tgcgcgccta tttcaagcat gatccgactg cgcgtgtccg
     3121 cgcgtttcct ggagaagggc aagatatacc gatctggctt cttggatcga gcgatttcag
     3181 tgcgcgtctt gcagcggaac tcggcttgcc gttttcattc gccagccatt tcgctccgac
     3241 gtatatgatg catgcgctga acttgtacca tcgcaatttc aagccttcga aggatctcgg
     3301 aaaaccgtac acgatgctag gcatcaacat catcgccgcg gataccgatg aaaaggcgca
     3361 atggctggca acttcccacc agcagatgtt cctgaatatc cgaagaggga tcacgacgga
     3421 attcaagcct ccgatcgatg acgtggatgc tgtctggaat gatcttgaaa aatcggcagt
     3481 cgccgaatta ttggagtggc catcgacgat tgtcggcagt cctgaaacgg tcaaaaagaa
     3541 attggaagcg ttcatggaca tgacgaaggc ggatgaatgc attatcagct cggggatctt
     3601 caaccatgaa gaccgtctcc gctcctatga aatcgtctcg gaaatgatgg catgacgaag
     3661 atgacttttg aataaaatag ccaagtatcg gacagcaccc gatgcttggc tattttcatg
     3721 ttttccttct ctgttcttgc tgagcaatca actttgtccc gacaaggtcg cccgtaatgt
     3781 tcagcattgt cgcccccatt ccgacgagcg cgtcgatggc ggtcagcaac gccactgctt
     3841 ccatcggcaa gccgagttgg acgaacacgg ttgcaatcat gacgatgcca gcgcctggaa
     3901 cccctgctgt acctatcgaa gcgagcgtcc cgatcaacac gaccatcaac atatcgggaa
     3961 tcgtaagcgg ggtgcctgca acattggcgg caaagacggc ggaaacggct attcggatcg
     4021 cagcgccgtc catattgaca gtagcgccga gcggcaggct gaagccgtat aagctttttg
     4081 gaatccccat ttttttcgcc gcatccagtg taagcggcaa tgtacctgtg ctgctttgcg
     4141 tgacaaatgc cgtgatcatt ggtgtgcgtg catatttgaa gaaaggtccg ggtttgattc
     4201 cgaacagaag cagtaggatg atgtacagca cgatatgaac gagcagtgca atataaaaga
     4261 cgccgatcat tccgcctaaa gaaacgagtg tatcgattcc ttggctgccg accgtatttg
     4321 cgatgatcgc aaaaatgccg atcggcacgt attgaaggat cgcttttaag atgatcaggg
     4381 atgcttcatt caaagccgta acgttcgtca ataatgaatc tccgagcccg ccatgttttt
     4441 cagatgagcg catgacggaa atcgcaatgc cgaatgcgag cgcagtgaag atgatgccaa
     4501 gcagattcat ttccgtaaat gcggtggcga tattggacgg aacgatgtta aggagcacat
     4561 gaatgatgcc ggggttttcc gggacatcga atgtttcatt ccccgtaaga tgcattcccg
     4621 tgccgggccg caaaatgctt gcaacgagca gcccgacgat aatcgcgagc gccgaagtga
     4681 cggtgtagta gatgaagact ttgctgccca tgcgccctag gttggcgata ttcgtctgat
     4741 tcaccccaac gatgagtgtg aacaaaataa gcgggatgat caggaatgtg agcagacgga
     4801 gaagcagatc gccgagcggt gcaagatgag ctgcttgttc ccctaacagg aaacctgcaa
     4861 tgattccgag aactaaggcg accgaaattt ttaaaatcaa tgaagcccgg tagtatgcca
     4921 tgaatatgtt tttcatcttg ccgcctcctt ttcagagaat caagtattga acgaattctg
     4981 aattctacgt taatacagtg cggtcggcgt gtcaaaatac atgctttggg taaagggaag
     5041 tcggtgcaac atgtcgaagg aataggagag gagggatcgt gatggtgcaa gtgcaagagc
     5101 aagcggatac ggttgaacgg aacgaattca gggaggcgcc tgttacgacg gtgttcaaga
     5161 agttcggaaa caataaggac gtctcgctcg ttttcgggga tccgattgaa gtgggactga
     5221 caaaagtggt gcctgttgcg aaattgagat acggattcgg cggcggaggc gatggcaagg
     5281 gtggtgacgg cggcggcggg ggctttgtcg ttaatccgat cggtgtctat gaaatcacgc
     5341 ccgatcgggt gaggttcaag ccggcacgca gcggtgggct agtgatcggt ttggcattgg
     5401 tgtccggtct attctttttc ctcggaagga aatcatgtaa aaaacggtga aagaacaagg
     5461 actctttttt tatgcaagag tcctttttcg tttcctatgg acggacgcgt ttccggatag
     5521 agccgatgac aaagccgata aagccgaata caatggcagg gacgatccag ccgatccctt
     5581 cttcgtaaag gggcaaatgg cggagcacat ggacaatcgg tgaaatgggc aggccgaacg
     5641 ccttcagcat atcgaaaaac ccgacaaatc cagctgcggc tagcgcgaat aaatacactt
     5701 ccctgtagcc gttgaaccag ttatgaaaga aactgatggt aatcaagaca atagccagcg
     5761 ggtaaatccc gataagaacc ggcaatgtaa gtgaaattaa gtgcgtaagc ccgatgttgg
     5821 caatgaacgt gctgaagacg cagacgatca atacgacata acggtacgtc aatttcggga
     5881 atgtgcccga gaaaaaggcg gcgcatgatg aaacaagtcc gaccgaagtc gtcatgcaag
     5941 caaggaagac agctgtgcca agcaaaactt ggccagtcgt cccgagcagt gaatagacga
     6001 gctgcgttaa aatttctccg ccatttgcag cattcggtgc aacgagccga cttgttgtcc
     6061 cgagataagc gagcccgaca tacacgaggc aaagcccgac aacggcaaag acgcctgcct
     6121 tcatcgtgat ggcgagggtc acccttcggt ccgaaatgcc tcttgcctta atcgcgttga
     6181 tgacgacgat gccgaacacg agtgcagcca tcgcgtccat cgtcaaataa ccctcgatga
     6241 atcctttgat gaatggatgt tggatgtatg gctcctgcgg cggtccactt gtcccgtcag
     6301 gtttgataat ccctgctatg acgatgaaag cgacgagggc gagaagaacc ggcgtcagca
     6361 cattaccgaa acggtcgacc aatttattcg gctttaatga tagccaagcg gtgaccgcga
     6421 aaaagaggac cgtaaataaa agcaaagcca tcctttctgc tgaatcggaa acgaagggag
     6481 ccagtcccat ttcaaacgtg accgttgccg tgcgcggaac tgcaaaaaat ggtccgagcg
     6541 ccaaatagac aacgatcgca aagacgaggc cgaacaatgg atgggccttg cccgcgaggt
     6601 ccctcaactg gctgccgctc atcgcgaccg caacgacacc gagaaacgga agaccgacac
     6661 cggtgatgag aaacccggca atcgcaatcc atacattgcc tcccgccagc tgcccgacga
     6721 gcggcggaaa gatcatattg ccggcgccga aaaacagcgc aaacatcaat aagcctaact
     6781 tcaatgtttc cttattcgaa aaatcattca taacaattcc ctccgacatt caaaaagcgc
     6841 ataactcgcg ctaaacacgc ataaatccaa tccctcaaaa gtccataaac gtaattaaag
     6901 gcgcataatt cttaataaaa ctccataaga gagtttacca catcccatac catatccatt
     6961 cctgacaatc attaaaaaga gaaaaattat taatttctga taataatcta tatacaagtt
     7021 tgaaagtatg ttaatattca gaagagagtt cgattttatt aaaatgtttt ggaatgagga
     7081 ggaataggga tgttgaatgt aacggaaaat cggtttgcga attatccgca gcagccacat
     7141 ggggggaaac tggtcgacaa agtgttgacg gggacggaaa gggaagagga gctggaaagg
     7201 gcgagattga tgccatccat catggtcgac ttggaagcgg tcatcacgct tgagatgatt
     7261 gcaacaggtg tgctatcgcc gaatgaaggg ttcatgaatg aagtcgacta taaatccgtc
     7321 ttgaatgaag gcaggctctc gaacgggacg gtttggccgg ttccgctcag cttcgcaccg
     7381 atcggcaacc gaaacaaaga aatcatcaaa acgctatccg tgggggacga agtggcgctt
     7441 tccgatgaac agaatgagcc tgtagcgatt ttgaagattg aagacatttt cgactatgat
     7501 aaagactatc gcgcttctca cctattcggc acaactgatc gcaaccatcc cggggtggat
     7561 gcgatattca ggcgcatggg cgatgtagcg ctaggcggac cgatcacgtt attgcgccgg
     7621 gtcgattggg ggccgttcga aaaacttcgt ttggagccga aagatacgtg gcatgaattt
     7681 tatgaagtga agaagttccg ttccgcggca gggttcatca caggggcgaa ccctttgcat
     7741 cggggccatg aatatatcca taaaaatgcg ttggaagaaa tcgatggcct gctccttcag
     7801 ccgttggtcg agatggcgaa acgcgaatat acgcgccatg agttccggat gctgtcgtat
     7861 cgtagtgtgt tggacacgta ttatccgaag g
//
LOCUS       DASULI010000216         4031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_653417, whole
            genome shotgun sequence.
ACCESSION   DASULI010000216 DASULI010000000
VERSION     DASULI010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653417"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFX34_04670"
     CDS             complement(<1..346)
                     /locus_tag="VFX34_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5564232.1"
                     /translation="MKRVPEVIRFDNVSFRYPDDEDWIFDNLSFSIDRGEKVVLTGPS
                     GSGKTTMLYLCNRLYPENCDGVVKGSVVLFDKESRSYLPGEVNHRIATVFQDPDAQFC
                     MPTVEEELAFTLE"
     gene            complement(330..911)
                     /locus_tag="VFX34_04675"
     CDS             complement(330..911)
                     /locus_tag="VFX34_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX5564233.1"
                     /translation="MLKSWKLKEVVLMSLFSVVFGIVYLLFLHVGNIWSGFIGPIAYE
                     WIFGIWFIVSIICMYIIRKPGAAVISETIAAAVEVLLGNAIGPRLILVGVIQGLGAEA
                     AFAATRYRRFDLWVLMLAGVGSSIFSFVYGYFLGGFTVFSTGYVLLMLGIRIVSGAVI
                     AGLGGKMVVDGLLATGSLRGYAITRVKKGEARA"
     gene            complement(913..1494)
                     /locus_tag="VFX34_04680"
     CDS             complement(913..1494)
                     /locus_tag="VFX34_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HEX5564234.1"
                     /translation="MECGLSPIVGFRFSLYPMADDFVSVIKGALEQTDTSNVWMHTDD
                     VSTVIRGKQVHVFNVAKAVALHAAKTGKHIALSGTFSAGCPGDTAGDAYLDKGEKIAN
                     TDATKQYVSSQFALYPMNNPAYMDIIYREVDRAKERGVYNESMHYASGIHGDIHDVFA
                     FYEEAFSKARSEEHKHLVMTVSMSINSPSHGGL"
     regulatory      complement(1573..1679)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1949..2431)
                     /locus_tag="VFX34_04685"
     CDS             complement(1949..2431)
                     /locus_tag="VFX34_04685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5564235.1"
                     /translation="MAMPTHIVACGGLVEDRNGNLLIVKTHHGGWVFPGGQVEVGENL
                     IDGLVREVKEESGIDVEVSHLIGVYTNSAIYKWYDGVTDVPTKVMFDFACKPVGGELC
                     TSEETSDCRWVPKDEVLEYIIQPAIRARIEEYFKFDGKVEYQEYVTHPEFDVKLNRKV
                     "
     gene            complement(2548..3546)
                     /locus_tag="VFX34_04690"
     CDS             complement(2548..3546)
                     /locus_tag="VFX34_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5564236.1"
                     /translation="MIGRNDPCPCGSGKKYKKCCQSKETITVETVQMEELEGILQTFY
                     DVYPERKDIPDYLELANEWKRSLNTYLPEEEMIEAICLDEFFFHHRPDIWTGYLDRQK
                     KKQVRPSVLKVIDTWRQPQAIIGEVIDVEEGYITVKSIFNGEVIRLRRESEKPVPVGV
                     HLFCFMLPDGTGVESQHLAVSSLIFFPTDHADVFKEFAEQFAAYEERDISKFLKENAM
                     KFWQLLGQNGYQGGEFTEFEAGVLLDAMDFLEKHDRMSRQLLDVVEDFLVEQQPNARK
                     ETAIAAGAIRFGQEHSFFEPLSMTLKEIAEWFDVSPSSMNKYYKELDVYYSSKVVS"
     gene            3765..>4031
                     /locus_tag="VFX34_04695"
     CDS             3765..>4031
                     /locus_tag="VFX34_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5564237.1"
                     /translation="MAATSNFIRLVGEHLRMIWESKGMNQEQLAEKAGIARARISEIE
                     NGKGNATLGTLAKVMEALEIAPMEIFNFQNLSDTNDITDKKLLIE"
BASE COUNT         1101 a         1086 c          776 g         1068 t
ORIGIN      
        1 tttcaagcgt gaatgcaagc tcctcttcga cggtcggcat gcagaactgt gcgtccggat
       61 cttgaaaaac agtcgcaatc cgatgattca cttctcccgg taaataggaa cggctttctt
      121 tatcaaatag cacaaccgag ccctttacaa ccccatcgca gttttccgga tacaaccggt
      181 tgcataaata gagcatcgtt gttttgccgc tcccgcttgg acccgtaaga acgactttct
      241 ccccgcggtc tatggagaat gaaagattgt cgaaaatcca atcctcatca tcgggataac
      301 ggaaactgac attgtcaaac cggatcactt caggcacgcg cttcaccctt tttcacccgg
      361 gtgattgcat acccgcggag cgagccggtc gcaagcaatc catcgacgac cattttaccg
      421 ccaagcccag caatcaccgc tccgctgacg atccggatgc caagcatgag cagtacatag
      481 cctgtgctga atactgtgaa accacctagg aaatagccgt agacgaagct gaagatcgaa
      541 gaaccgaccc ctgccagcat caacacccat agatcgaagc gccgatagcg cgttgcagcg
      601 aatgccgcct cggcgcctaa tccttgtatt acacctacaa gtatcaaacg tggaccgatt
      661 gcattcccca acagcacttc gacagccgcg gcgattgttt ccgaaatgac agccgccccc
      721 ggtttccgga taatatacat gcagatgatc gacacgatga accaaatgcc gaaaatccat
      781 tcataagcga ttggcccgat gaaccccgac catatattgc caacatgtag aaacagcaaa
      841 tagacgatcc cgaagacaac cgaaaacagc gacatgagga cgacctcttt caacttccat
      901 gacttcaaca tgttacagcc ctccatgcga cggactgttg atgctcatcg aaaccgtcat
      961 gacgaggtgc ttatgctctt ccgaacgcgc tttgctgaaa gcctcttcgt aaaatgcgaa
     1021 cacatcatgg atatctccgt ggatgccgct tgcataatgc attgattcgt tatagacgcc
     1081 tctctctttc gcccggtcca cttctcgata aatgatgtcc atatacgccg gattattcat
     1141 cggatagagc gcaaattggg aggagacata ttgcttcgtc gcatccgtat tcgcgatctt
     1201 ctcgccttta tccaaatacg catcgcccgc cgtatcgcct gggcagccag ccgaaaacgt
     1261 acccgataac gcaatatgct tgcccgtttt cgcggcatgc agcgcaaccg ccttcgccac
     1321 gttgaacaca tggacttgct ttccgcgaat gacggtggat acgtcatcgg tatgcatcca
     1381 cacatttgac gtatccgttt gttctagcgc ccctttaatt acacttacaa aatcatctgc
     1441 catcggatat aacgaaaagc gaaatccgac gattgggctt aatccacatt ccatttcctt
     1501 cgacctcccg aaaaaatttc ataaaaaaaa ctacatcccg gaagatgtag cacttctcca
     1561 atatgaaagc atcggaaagc cactacactt ccccacgcag gtattatcct gatcgggtca
     1621 taagggatcg gagctcttgc tcgcatctca gccagtattg ggcaccccta gtgcagaaac
     1681 ggtatgtagt tgttatttat gttgccatta gtatagacga agaatttttt attgtaaatg
     1741 aaatctggaa aactcgtact tttgtccgta tcgtatattc atatacagta cctttcttgc
     1801 agatacgcat aaaatcgttt attcgcgcat aaatcaaatc tttcacgcat aaattcatga
     1861 aagcgcgcat aaacgtaact acatgctcat aaatcataat aatggcgcat aaagctattt
     1921 attcacgcat aaatgcttcg gccatcactt acactttcct gttcaatttc acatcaaatt
     1981 cagggtgcgt gacatattcc tgatattcca ctttcccgtc aaacttgaaa tactcctcta
     2041 tgcgtgcccg gatcgcgggt tggataatat actcaagcac ttcatcctta ggaacccatc
     2101 ggcaatccga cgtttcctca gacgtgcaca attcccctcc gacaggtttg catgcaaagt
     2161 cgaacatcac tttcgtcggg acgtccgtca ctccgtcata ccatttataa atggccgagt
     2221 tcgtatacac tccaatcaga tgggacactt ccacgtcgat gccgctttcc tctttcactt
     2281 cccggaccag cccatcgatc aggttctccc cgacttcaac ctgcccgccc ggaaataccc
     2341 atcctccatg atgcgttttg acaatcaaca aattcccatt cctatcttca acaagccccc
     2401 cacaggctac gatatgcgtc ggcattgcca tttccccatc cccttttctc tgcatataaa
     2461 ctaatactac caattttccg ccaataaaga aagattctaa aaaatccacc tatcgcaatg
     2521 aaagcaatag gtggatcgtt ttttgtatta actcactact ttggagctgt aataaacatc
     2581 caattcttta taatatttat tcatagatga aggggagaca tcaaaccact cggcgatttc
     2641 cttcaatgtc atcgaaaggg gttcgaaaaa cgaatgctcc tgcccgaatc ggatcgcccc
     2701 ggcggcaatc gctgtttctt tacgggcatt cggctgttgt tccactaaaa agtcttcgac
     2761 aacatcgagt aattgccttg acattcgatc atgtttttca agaaaatcca ttgcatcgag
     2821 cagcacaccc gcctcaaact cagtgaactc tccgccttgg tatccgtttt ggccgagcag
     2881 ctgccagaat ttcatcgcat tttctttcaa gaacttgctg atgtcgcgtt cttcataggc
     2941 tgcaaactgc tctgcaaatt ccttgaagac atccgcatga tctgtcggga aaaagatcag
     3001 gctcgacacg gccaaatgct gactttccac tcctgtaccg tcaggcagca taaagcagaa
     3061 taagtgcaca ccgactggca ccggcttctc actttcccgt cgcaaccgga tgacttcacc
     3121 gttaaagatt gatttcaccg taatgtaacc ctcttcaaca tcaatcactt cgcctatgat
     3181 tgcttgcggt tgccgccacg tgtcgatcac ctttaacacg gaagggcgta cttgcttttt
     3241 cttctgtctg tccaaatagc cggtccaaat gtctgggcga tgatggaaaa agaattcatc
     3301 caaacaaatc gcttcaatca tttcttcttc cggcaaatat gtattcaatg aacgtttcca
     3361 ttcattcgcc agttccaaat agtccgggat gtctttccgc tcaggataca catcataaaa
     3421 tgtctgcagg atcccttcca attcttccat ctgaaccgtt tcaacggtaa tcgtctcttt
     3481 cgattgacag catttcttat atttcttccc gctgccgcaa gggcaaggat cattacgtcc
     3541 aatcatttca aacacttcct tcataaaaat agaacctatc cagtttacca tttttctggc
     3601 gtttctggaa cggtcccaat ttcatgtttt gcggaatgaa ttacttcttc ctagaatgtg
     3661 cttttactgt acgttatata gtacattgat gattctttgt attttttatg cggataatca
     3721 ttttccatct attcctctaa cctcaaaggt agaggtgata cgttgtggca gctacttcaa
     3781 acttcatacg gcttgtcgga gaacatcttc ggatgatctg ggaatcaaag ggaatgaacc
     3841 aagaacaatt ggccgaaaaa gcgggaattg caagggcccg aatttctgaa atcgagaacg
     3901 gaaaaggcaa tgccacatta ggtacgctgg caaaagtcat ggaagcgctg gaaatcgcac
     3961 cgatggaaat attcaacttc cagaacttat ccgacacaaa tgatatcaca gacaagaagc
     4021 tattgatcga a
//
LOCUS       DASULI010000217         4558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1480637,
            whole genome shotgun sequence.
ACCESSION   DASULI010000217 DASULI010000000
VERSION     DASULI010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480637"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFX34_04700"
     CDS             complement(<1..299)
                     /locus_tag="VFX34_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5564238.1"
                     /translation="MDFRVFIIAISTILVGLVELIVGGILPIIADDLNVSIGTAGQLI
                     TVYALVFAIAGPVLLSLTAKVERKKLYLLSMAVFFAGNILTYLSPTFAFLMIARIL"
     regulatory      complement(376..475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            590..1105
                     /locus_tag="VFX34_04705"
     CDS             590..1105
                     /locus_tag="VFX34_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564239.1"
                     /translation="MHDPTIFENLKVAFENRIYDLDTIKREIRITHRSDQMDFAVLAR
                     KFAIQFTLFDQEEVSAEIILGASLRDLADEILETPGGNPGTSLTVRFQKHVQEAATQC
                     PQIEEVLSDIWENEVLVIQTLSFMYGDDEFGYLNNIEVKFRTQINEENMQEIPDFLHS
                     VLDALRVLHAI"
     gene            1345..2901
                     /locus_tag="VFX34_04710"
     CDS             1345..2901
                     /locus_tag="VFX34_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX5564240.1"
                     /translation="MSLLTVSNLSHGFGDRAIFDDVSFRLLQGEHIGLIGANGEGKST
                     FMNIITRKLEPDAGTVAWAKRVRVGYLDQHVVLQQGMSIRDVLRTAFQYLYDLEAEMN
                     ALFAKMGEVDADELEKLLEETGQMQDELTNNDFYIIDSKVDEVANGLGLDEFGLERDV
                     HDLSGGQRTKVLLGKLLLEKPDILLLDEPTNYLDVEHIEWLRNYLQNYDNAFILISHD
                     IPFLNSVINLIYHMENQQITRYAGDYDEFLRVHEMKKQQVEAAYKKQQKEIANLKDFV
                     ARNKANAATSRMAMSRQKKLDKMDIIELDAEKPKPQFDFKLARTPGRYLFQTKGLVIG
                     YDEPLSRELDLTMERGQKIALSGANGIGKTTLLRSILGEIPSLAGSVELGDHLEIGYF
                     EQEMKTESNRTCLEEVWDEFPHFTQYEVRAALARCGLTTKHIESKVKVLSGGERAKVR
                     LCKLINRETNLLVLDEPTNHLDVDAKTELKRALKEFKGSVLLISHEPDFYEGVVTDVW
                     NGENWTTKMF"
     gene            complement(3081..3299)
                     /locus_tag="VFX34_04715"
     CDS             complement(3081..3299)
                     /locus_tag="VFX34_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein tlp"
                     /protein_id="HEX5564241.1"
                     /translation="MPRPLPNDSADNVDRLKKTISNMEAANEAAKTAEGAELASIKES
                     NERRKEAIEGLEEEIQQENKSRINGYIE"
     gene            3356..4219
                     /locus_tag="VFX34_04720"
     CDS             3356..4219
                     /locus_tag="VFX34_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564242.1"
                     /translation="MQTAKLVLFFMTTPIKRQEPSNNWYVRQEDKKCRGDLFMKNTWK
                     KTGAALLIAGLTVSGTGYALADGGKVTEIIDLSKLDENTVEETPVVPVKEVEETTDTE
                     TDVNEDADTETDNVTDSETDKEEGTETDKEDGSKESDQEEATETDELPEIPEGYTAGN
                     LTALAKAYEQAGNPTAKAAIKRNMERSIAKWEAKQPVVETPEEEVKEEAPATEDTTKV
                     EEPVKEEVPVQEEKPAKVKEKNPQAELNAKHKEQREELKTAQKAERDALKEERKAAKE
                     EQKQEKKEKKN"
     gene            4304..>4558
                     /locus_tag="VFX34_04725"
     CDS             4304..>4558
                     /locus_tag="VFX34_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HEX5564243.1"
                     /translation="MLLSIIQGIFNKKKSDTALNELVWKAKSGDEKVMNDLLIAFTPF
                     MKKTASFVWDRFIDESDEEFSVAMVGFHEAVLKYKPEENAS"
BASE COUNT         1388 a          903 c         1156 g         1111 t
ORIGIN      
        1 agtatacggg cgatcatgag aaatgcgaaa gtcgggctga gataggtcaa tatattgccg
       61 gcaaaaaaga ctgccattga tagtaaataa agttttttcc tctcgacttt tgcggttaat
      121 gatagtaaaa ccggaccggc aatggcaaac acgagcgcgt aaacggttat gagctgcccg
      181 gccgtcccga tggaaacgtt caaatcatct gcaataatcg gcaaaatccc gccgacgatc
      241 aattcaacga gaccgactag aatcgttgat atggcaatga tgaaaactct aaagtccatg
      301 ggaactcctc ctgttggtct atttatagtg tgaacctttt atgcaaaata attttcggaa
      361 aataaaaaac ccgactacaa agtttgtttc ctttgtagtc aggagttcat ggttcctggt
      421 agagaccctc aaaccgtatt attgaggtta tacatatatg ttgtctttat gaaattttcc
      481 attcaccaat ctaacatgat tcgagatata attcaagagc aatttccttt ttatatccga
      541 ttatggttac atagaaaata acaatcaaat ttgttctgga ggaatcgata tgcacgatcc
      601 aaccatattt gagaacttaa aagtcgcatt tgaaaaccgg atatacgatc tagatacaat
      661 caagcgcgaa ataaggatca cccatcgatc ggatcaaatg gattttgctg tcctggcaag
      721 gaagttcgct attcaattta cgcttttcga tcaagaagag gtgtcggccg agatcatact
      781 gggggcatct ttacgcgatt tggcggatga aatactggaa acgccagggg gaaatcccgg
      841 cacttcattg actgtacgct tccaaaagca tgttcaagaa gcagctacgc aatgtccgca
      901 aattgaagaa gtgttaagtg acatttggga aaatgaagtg ctcgtcattc aaacattgag
      961 tttcatgtac ggcgatgatg agttcggtta tttgaacaat attgaagtga aattcaggac
     1021 gcaaatcaat gaggagaata tgcaggaaat ccccgacttc ctgcatagtg tccttgatgc
     1081 gttgcgtgtg ctgcatgcca tttgaaaaaa acctatgccg atgagtggaa tcgctatata
     1141 atgaaggaca aatgcgttat gcgcatttgt gagcatttat gcgtcgattt tgcgatttat
     1201 gcgctaaaaa atcggtttat gagcttttgg agtgttttat gcgggtattt gcggttttat
     1261 gcgtgtttcg gcggctttat gcgtggttac aaagatttat gcgtgtttgg aattaatttt
     1321 aacgaaggaa taggtgcaca atatatgagt ttattgacag taagtaattt aagccatggt
     1381 ttcggggatc gggcgatttt cgatgatgtg tcgttccgtt tattgcaagg cgagcatatc
     1441 gggttgatcg gtgcgaacgg ggaagggaaa tccacgttca tgaacatcat cacccggaag
     1501 ttggagccgg acgcgggaac agtcgcatgg gcgaagcgcg tgcgcgtcgg ttatttggat
     1561 cagcatgtcg tgctacagca ggggatgagc atccgtgatg tgctccggac agcatttcaa
     1621 tatttgtatg atctcgaagc ggaaatgaat gccttgttcg cgaagatggg cgaagtcgat
     1681 gcggacgagc ttgagaagct gctagaggaa acggggcaga tgcaggacga gctgacgaac
     1741 aatgactttt acatcatcga ttcgaaggtt gacgaggtgg cgaacggact cggcttggat
     1801 gaattcggcc tcgagcgcga tgtccatgat ctaagcggcg ggcagcggac gaaagtgctg
     1861 cttggcaagc tattgcttga gaagccggac atcctcctcc tggacgagcc gacgaactat
     1921 ttggatgtcg agcatatcga atggctgcga aattatttgc agaactatga taatgcgttc
     1981 atcctcattt cgcatgatat cccgttcctg aacagcgtca tcaacttgat ctaccatatg
     2041 gagaaccagc agattacacg gtacgccggg gattatgatg agtttttacg tgtccatgaa
     2101 atgaagaagc agcaagtgga agcggcttat aagaagcaac agaaggaaat cgccaacttg
     2161 aaggactttg tggcgcgcaa taaagcgaat gccgcgacga gccggatggc gatgtcacgc
     2221 cagaagaagc tagataagat ggacatcatc gaactagacg cggagaagcc gaagccgcaa
     2281 ttcgatttca agcttgcccg tacgccggga cgttatttat tccaaacgaa aggccttgtc
     2341 atcgggtacg atgagccgct atcgagggaa ctcgatctga cgatggagcg cggacagaag
     2401 attgcgctgt ccggtgcaaa tggaatcggg aagacgacgc tattgcggag catccttggt
     2461 gaaatcccgt ctcttgcggg ttctgtcgaa cttggcgacc atcttgaaat cggctatttc
     2521 gagcaggaaa tgaaaacgga atcgaaccgc acttgtttgg aagaagtgtg ggatgaattc
     2581 cctcatttca cacagtatga agtgcgtgcc gcgttggcgc gatgcggctt gacgacgaag
     2641 catatcgaga gcaaggtgaa agtgctgagc gggggagaac gggcgaaagt gcgcctctgc
     2701 aagctcatca accgggaaac gaatctgctt gtcctcgacg agccgacgaa ccacctcgac
     2761 gtcgatgcga aaaccgagtt aaaacgcgct ttgaaggaat ttaagggcag cgtactgctc
     2821 atttcccatg aacctgattt ttatgagggc gtcgtgacgg atgtatggaa cggtgaaaat
     2881 tggacgacga aaatgtttta atgcaaaagc gaaagccgtt acggagaacg gcttttgcga
     2941 gcacaagcgc agcgttgcga gcaagaaatg caatcagcgg ttttttgaaa tagagcaact
     3001 ttgacttatg accccgagcc tatggcgcca gacgtgaata aatagagcac ctgagcgaca
     3061 tacgatcgcc caggtgtttt tcattcaatg tagccattga tccgtgattt gttttcctgc
     3121 tggatttcct cttctaatcc ttctatcgct tctttacggc gctcattact ttccttaatg
     3181 gaagcgagct ctgcaccttc cgccgttttc gctgcttcat tcgccgcttc catattgctg
     3241 attgttttct tcaagcggtc gacgttatcg gcagagtcgt ttggcaatgg tctaggcatg
     3301 atcattcctc cttctttatt caatcaaata gtctttccaa gtaaagaaaa aatatgtgca
     3361 aactgctaaa ctcgtactat tttttatgac gacccccata aaacgacaag aaccttcgaa
     3421 taactggtat gtccgacaag aggacaaaaa atgtcgaggt gatttattta tgaaaaacac
     3481 atggaagaaa acgggagcag cattacttat cgcaggattg acagtaagtg ggacaggtta
     3541 tgcactagct gacggaggaa aggtcacgga aatcatcgat ctaagcaaac tagacgaaaa
     3601 cacggttgag gaaacgccag ttgtccctgt taaggaagtt gaagaaacta ctgacacgga
     3661 gacagatgta aatgaagatg ctgacacgga aacagataac gtcactgatt ctgaaactga
     3721 caaggaagaa ggaacagaaa ctgacaaaga agatggatca aaagaatccg atcaagaaga
     3781 agcaactgaa acagatgaac ttcctgaaat tccggaaggc tatacagcag gaaatcttac
     3841 tgctctagca aaagcatatg agcaagctgg aaatccaact gcaaaagctg ccatcaaacg
     3901 caatatggag cgctccattg caaagtggga agcaaagcaa cctgtagtag aaactcctga
     3961 agaggaagtt aaggaagaag cgccagcaac tgaagataca actaaagttg aagagccagt
     4021 gaaagaagaa gtacctgtac aagaagagaa gcctgcaaaa gtgaaagaga aaaatccaca
     4081 agctgaattg aatgcaaaac ataaggagca acgtgaagaa ttgaaaactg ctcaaaaagc
     4141 tgaacgcgat gctttgaaag aagagcgtaa agctgcaaaa gaagagcaaa aacaagagaa
     4201 aaaagagaag aaaaactaat ttaccagagt ttactgcttc ctattttccg ttgccccatt
     4261 tacaatcgaa gcaatcggaa ttgatgggaa cggagggttg tcgatgcttt tgtccatcat
     4321 acaagggatt ttcaacaaga agaagtctga tacagcattg aatgagcttg tatggaaggc
     4381 gaagagtggc gatgagaagg tcatgaacga tcttctcatc gcatttactc cgttcatgaa
     4441 aaaaacggct tctttcgtat gggatcgatt catcgacgaa agcgatgagg aattcagcgt
     4501 ggcaatggtc ggctttcacg aagcggtttt gaaatacaaa ccggaagaaa atgcgtcg
//
LOCUS       DASULI010000218         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_845074, whole
            genome shotgun sequence.
ACCESSION   DASULI010000218 DASULI010000000
VERSION     DASULI010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845074"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFX34_04730"
     CDS             <1..266
                     /locus_tag="VFX34_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX5564244.1"
                     /translation="FAEQPGALLKQGNGLFASDGNLPEAQNMAGVGYSFQQGYLESSN
                     VDAAKAMTDMLAAYRAFEANQKILQAYDRSMEKAVNEIGRVNG"
     gene            292..1128
                     /locus_tag="VFX34_04735"
     CDS             292..1128
                     /locus_tag="VFX34_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HEX5564245.1"
                     /translation="MIRSMTTAVNTMNQLQSQFDIIGNNLANSATNGYKSNEANFHEL
                     LYQQFNNDKADTAQRLSPAGIRYGVGAALGPAKMNWTVGSLQMTGRELDFALTEPKQY
                     FNLIMPGEDGEQTVYTRAGNFYVSPVDGGNLMLVNSDGYPVADSAGQPITFADTVKNY
                     NMLPGGVLEVMHNNGNVQQIEMGVTTLERPNLMSRLSSTLLALPDNLADLGVGAGDVL
                     TEMNGVNRNRIGMENQKLEASNVNYEKEMTDLINVQRSYQFNARTVTLADQMLGLINN
                     IR"
     gene            1151..1486
                     /locus_tag="VFX34_04740"
     CDS             1151..1486
                     /locus_tag="VFX34_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5564246.1"
                     /translation="MIDEKQKHPQWDHMKQKDATERQIVASRVKRSQQKKQKSEEEEA
                     VSKRRIWVQIRLFPIWLRIVLVLLLIAGAAILGAIIGYGYVGDGVPSDVLKKETWTHI
                     LDIINGKES"
     gene            1538..1975
                     /gene="fabZ"
                     /locus_tag="VFX34_04745"
     CDS             1538..1975
                     /gene="fabZ"
                     /locus_tag="VFX34_04745"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5564247.1"
                     /translation="MLNAEQIQEIIPHRYPFLLVDRITEIEEGKRVVGLKNVTINEDF
                     FNGHFPGYPVMPGVLIVEALAQVGAVAILQKEENRGRLAFFAGIDNCRFKRQVVPGDQ
                     LKLEVEITRMRGSIGKGKAVATVDGEIACETEITFALGPVASN"
     gene            2074..2466
                     /locus_tag="VFX34_04750"
     CDS             2074..2466
                     /locus_tag="VFX34_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564248.1"
                     /translation="MYNDLSPNIKSSITRSITQTFEQYMADIGWDEELYDIEDFIDSW
                     REYITTKALWYSRIPEDLKREPKFHEDLAQRINEVIQRIISEPPSDEQIANIQIMQEK
                     LNTHLTYDCKAEAVYVETLLKDRLEKEQ"
     gene            2556..2918
                     /locus_tag="VFX34_04755"
     CDS             2556..2918
                     /locus_tag="VFX34_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564249.1"
                     /translation="MFKKAIPFILVSGLALTACTNNNTVPKNNETPMQNLERDLTPRV
                     NNGAGPNMDGVDNGIDRTNGVNNGVINNGRNGLNNDRLNGDMNGDNTTAPNEEIIIDE
                     NVTTPNGTNTNRNRNNNR"
     gene            complement(3179..>3627)
                     /locus_tag="VFX34_04760"
     CDS             complement(3179..>3627)
                     /locus_tag="VFX34_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwpF family protein"
                     /protein_id="HEX5564250.1"
                     /translation="MVKTFKMISVGILIDDKIQDFPLVDGIIINQENSHRMWILELFI
                     DQEHQQLFEGWKESNELLEARVVISYPENEPAGFRVAVDAVQQIGDHISVLLKGRLKR
                     ARTQYAEHLLEELITEGLSGEELLKRFESDMRERPRLKKDREEAKE"
BASE COUNT         1056 a          920 c          874 g          777 t
ORIGIN      
        1 cattcgcgga gcagccgggc gctctcctca agcaaggaaa cggcctgttt gcaagcgatg
       61 gcaacttgcc tgaagcgcag aatatggcag gcgtcgggta ttcattccag cagggctatc
      121 tggaaagctc caacgtcgac gcggcgaagg cgatgacgga catgcttgca gcataccgtg
      181 cgttcgaagc gaatcaaaag atccttcagg catatgatcg aagcatggaa aaagctgtga
      241 atgaaattgg acgtgtcaac ggatgatgcg ttttgaaagg ggcagaaacg aatgatacga
      301 tcaatgacga cagcagtgaa cacaatgaac caattgcaaa gccaattcga tatcatcgga
      361 aataaccttg caaattcggc gacaaacggg tataaatcga atgaagccaa ttttcatgaa
      421 ctgctttacc agcaattcaa taacgataaa gccgatacgg cacagcgcct atccccggca
      481 ggcatccgct atggcgtggg ggcggcactt ggtccggcaa aaatgaactg gacggtcggc
      541 tctttgcaga tgaccggacg cgaactcgat ttcgcattga ccgagccgaa gcagtacttc
      601 aaccttatca tgcccggaga agacggggag caaacggttt acacacgtgc cgggaatttt
      661 tacgtatcgc ccgttgacgg cggcaacctg atgctcgtca atagtgatgg ttatccagtc
      721 gcggacagcg caggacagcc gatcacattt gccgatacgg tgaaaaatta taatatgctt
      781 ccgggcggcg tcctcgaagt gatgcacaac aacggaaacg tgcagcagat tgaaatgggc
      841 gtcacgacgt tggagcgtcc gaacctgatg agccgcctat cctcgactct actggcgctt
      901 cctgacaact tggcggacct aggcgtgggc gccggtgacg tcctgacgga aatgaacggc
      961 gtaaaccgga accgcatcgg catggaaaac cagaaactcg aggcatccaa cgtcaattac
     1021 gaaaaagaga tgacagacct gatcaatgtc cagcgttctt accaattcaa cgcgcggacg
     1081 gtcacgcttg ccgatcaaat gttaggcttg atcaataaca ttcgataagc cgcgctaggg
     1141 agaatccgat atgatagatg aaaaacagaa acatcctcaa tgggaccata tgaaacaaaa
     1201 agacgccacc gagcggcaaa tcgtcgcttc gcgcgtcaaa cgcagccaac agaagaagca
     1261 gaagtccgaa gaagaggaag cggtcagcaa gcggcgtata tgggtccaaa tcagactgtt
     1321 cccgatctgg ctgaggattg tgcttgtcct gctgttgatc gcgggcgctg cgattcttgg
     1381 agccatcatc ggctatggct atgtcggaga cggcgtgcct tccgatgtac tgaaaaaaga
     1441 gacgtggacg cacatactcg atatcataaa cgggaaagag tcgtaacggc tctttcgttt
     1501 attttaaatt acataaattg tcgggaggat aaaagtcatg ctgaacgcag aacagattca
     1561 agaaatcatt ccccatcgct atccattcct attggtcgat aggattacgg aaatcgaaga
     1621 agggaaacgg gtcgtcggtc tgaaaaacgt caccatcaac gaagactttt tcaacggcca
     1681 ctttccgggc tacccggtca tgccgggcgt cctgatcgtc gaagcgcttg cccaagtcgg
     1741 cgctgttgcc atccttcaaa aagaggaaaa ccgcggtcga ctcgcattct ttgcgggaat
     1801 cgacaattgc cggttcaaac gccaagtcgt ccccggcgat caattgaagc tggaagtcga
     1861 aatcactcgg atgcgcggct ccattgggaa agggaaagcg gttgcgaccg tagacggcga
     1921 aatcgcttgc gaaacggaaa ttacatttgc actaggacca gtagcttcaa attgatttgc
     1981 gcacgtgcaa aatgtcagct acaatggaaa atgcagtgtg tttattgaaa ctttcagcac
     2041 atgcatcttt acagtgggag gttccaacca attatgtata atgatctttc tccaaatata
     2101 aagagcagca tcactcgctc catcacgcaa acgttcgagc aatacatggc ggacatcgga
     2161 tgggacgaag agctatacga tatcgaagac ttcatcgatt catggcgcga atacatcacg
     2221 acaaaagcac tatggtattc acgaattccg gaagacttga aaagagagcc gaaattccac
     2281 gaagacctcg cccagcgaat caatgaagtc atccagcgca tcatcagcga gccgccatcc
     2341 gatgagcaga tcgccaacat ccagatcatg caggaaaaac tcaacacgca tttgacatac
     2401 gactgtaaag ccgaagccgt ttacgtcgaa acgctgctca aggatcgcct cgaaaaagaa
     2461 caatgaaaaa gtatggatag ttccctcctt tttagccaac ctacaagtat catacgattt
     2521 gtatgataga ggcttgtgga aaggagggaa ttttcatgtt caaaaaagcg ataccgttca
     2581 tactcgtttc cggacttgca ttaacggctt gtaccaacaa caacaccgtt ccgaaaaaca
     2641 acgaaacgcc gatgcagaac ctggaacggg acttgacccc gcgtgtcaat aacggcgcag
     2701 gaccgaacat ggatggcgtc gacaacggaa tcgatcggac caacggtgtt aacaacggag
     2761 ttatcaataa tggccgtaac ggacttaata acgaccgtct taacggagac atgaacggtg
     2821 acaacacaac ggcaccgaac gaagaaatca ttattgatga aaacgtaaca actccaaacg
     2881 gcaccaacac caatcggaac cgcaacaaca accgctaacc gcaagccaaa agaaaaaacc
     2941 gcctcccccg gcggtttttc ttgtttcaca gaatgttcca cggtacctgt gtgacacact
     3001 actcagttta ttcactacaa ttgtatatta gggaaattga aacatagtca tatagagaag
     3061 aggatgacgt ttcattgtat tttcattctt ttcaatgaat tgtcataaat gcgccttgca
     3121 caggtaccga cacaattaag ccaaaagaaa aaccgcctct cccggcggtt tttcttgttt
     3181 attccttcgc ttcttcgcga tccttcttca accgcgggcg ctcccgcata tccgattcga
     3241 agcgcttgag cagctcctcg ccgctcagtc cctcggtgat caattcctct agtagatgtt
     3301 ccgcatactg cgtgcgggcg cgcttcagcc ggcccttcag caggaccgaa atatgatccc
     3361 cgatctgttg cacagcatcg accgcaacgc ggaatccagc cggctcgttt tccggatacg
     3421 aaatgacgac gcgcgcctcc aataactcgt tcgactcctt ccaaccctcg aacagctgct
     3481 gatgctcctg atcgatgaaa agttccaaaa tccacatccg gtggctattc tcctgattga
     3541 tgataatgcc gtcgaccagc ggaaagtcct ggattttatc atctatcaat ataccgaccg
     3601 aaatcatctt aaatgtcttc accatgc
//
LOCUS       DASULI010000219         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1096745,
            whole genome shotgun sequence.
ACCESSION   DASULI010000219 DASULI010000000
VERSION     DASULI010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096745"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFX34_04765"
     CDS             <1..788
                     /locus_tag="VFX34_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX5564251.1"
                     /translation="IHYGLTMREHQSLIRKQEAIQMEMNVATKVQDTLLKTKMPCVGG
                     LDVGYISQPAKQMNGDYVYFLNKKHESGVAVADVIGKGIPAALCMSMIKFGMDGLPDG
                     NTSPQNVLGIINRIVEKSVDDSMFISMFYGKYNSEDTTFSYASAGHEPALHFDSGNDV
                     FSELDAKGLLLGVKPDAEYEERSVVLNDGDFIVMMTDGVTETRTENGFIDMNTIQNLL
                     RNVKDKTAQQIAEHVYHNLSELQNHQLGDDFTIVIFKKEPEQV"
     gene            861..1202
                     /locus_tag="VFX34_04770"
     CDS             861..1202
                     /locus_tag="VFX34_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301455.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEX5564252.1"
                     /translation="MNLQVELLEQDSVQYFKVVGEIDAFTAPVLRERLAAVENVQGLK
                     AELDLSEVDYMDSTGLGVFVGFYKAVSANGGHVKITGLNKRLNRLFEITGLNEIIDIE
                     QKESEDNNATL"
     gene            1189..1662
                     /gene="rsbW"
                     /locus_tag="VFX34_04775"
     CDS             1189..1662
                     /gene="rsbW"
                     /locus_tag="VFX34_04775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HEX5564253.1"
                     /translation="MQHYDYIEIKIPAKAQSVGVARLMISGIASRLGFTYDDIEDLKI
                     ASSEAITNAVQHAYGEAEDGEVVVGCALYEERLEIMIADHGTSFDFEETKKHIGPYSD
                     TSDARFLREGGLGLYLMETLMDEVKFHHEEGVTVFMTKYLGRERGEEDVKRTANS"
     gene            1637..2422
                     /gene="sigB"
                     /locus_tag="VFX34_04780"
     CDS             1637..2422
                     /gene="sigB"
                     /locus_tag="VFX34_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HEX5564254.1"
                     /translation="MSNEQPTHKPDTKEKVLEWIRQYQETNDDEAQTNLVLHYERLVH
                     SIARKYSNGKPYHEDIVQVGMLGLLGAIRRYDPELGRSFEAFAVPTIVGEIKRFLRDK
                     TWAVHVPRRIKELGPKIKAAVETLTIEMQRSPLVSEIAEYLDTDEELVLEAMEMGRSY
                     QALSMDHTLEADSEGGTVTLFDIIGESDDGYERTDHRLVVANALNVLSEREKQIIQYT
                     YIEQLSQKEAGERLGISQMHVSRLQRKAIKKLQEAIVAAGGAS"
     gene            2422..3018
                     /locus_tag="VFX34_04785"
     CDS             2422..3018
                     /locus_tag="VFX34_04785"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX5564255.1"
                     /translation="MEVFKNDHVEAYIYNAAKHGNIESGDTYYVHSEEEYFICAIADG
                     LGNGAVARQSAEVIPQVLEHYHHESLDELLNRCNEHMVQKRGAAVAIVRVNYKEGTIE
                     YSCVGNVRLYILHGHEKMIYPLPVMGYLSGRPQKMNTQKYKYEVGDLFFLHSDGITLN
                     SPKASLKQSSNAYDLYNTIKKSAQSGDDATFIAGSLLQ"
BASE COUNT          957 a          654 c          849 g          743 t
ORIGIN      
        1 tgatccacta tggtctaaca atgcgggaac accaaagcct catccgtaaa caagaagcga
       61 ttcaaatgga aatgaatgtc gcgacgaaag tgcaggacac acttctgaaa acgaagatgc
      121 catgcgtcgg cggcttggat gtcgggtata tttcccagcc cgctaaacag atgaacggcg
      181 attatgtgta tttcttaaat aagaagcatg aatcaggcgt tgctgtggca gatgtgatcg
      241 gcaaagggat tccggcggca ttatgcatgt ccatgatcaa attcggcatg gatggcttgc
      301 cggatggaaa tacgagtccc caaaacgtgc ttggcatcat taaccggatc gtcgagaaga
      361 gcgttgacga ttcgatgttc atatcgatgt tttacggaaa gtataattcc gaggatacga
      421 cattctccta cgcttctgct ggccatgaac cggcattgca tttcgatagc ggaaatgacg
      481 tcttttcgga attggatgcc aaagggctgc ttctcggcgt caaaccggat gccgagtatg
      541 aggaacgctc ggtcgtgctg aacgacgggg acttcatagt gatgatgacg gacggcgtta
      601 ccgaaacgag gaccgaaaat gggttcattg acatgaatac gatccagaac ctacttcgta
      661 atgtgaaaga caaaaccgcc cagcaaattg cggaacatgt ttatcacaat ctttcggagc
      721 ttcaaaatca tcaactgggc gatgacttca cgattgtcat ttttaaaaag gaaccggaac
      781 aggtttaatt ttttgagtgg cgggtattaa aggatgtagt gaattatcca atacaaaaat
      841 cgagagtcgg ggtgcaggaa atgaatttac aagttgagtt gctagaacaa gatagtgttc
      901 aatatttcaa ggttgtcggg gaaattgatg catttacggc acctgttctc agggaacggc
      961 tagcggcagt ggaaaatgtc caaggcttga aggccgaact ggatctatct gaagtggact
     1021 acatggatag tacaggtctc ggtgtttttg tcggttttta taaagctgtt tctgctaacg
     1081 gcgggcatgt gaagataacg ggcttgaaca aacggttgaa ccgtctattt gaaattaccg
     1141 gtttgaatga aattatcgat atcgaacaga aggaaagtga ggacaataat gcaacattat
     1201 gattatatcg aaatcaaaat acccgcaaaa gcccaatctg tcggtgttgc aagattgatg
     1261 atttcaggaa tcgccagccg gctaggattt acatacgatg acattgaaga cttgaaaatc
     1321 gcatcgagtg aagcaatcac gaacgctgtc cagcatgctt acggtgaggc ggaagatgga
     1381 gaagtcgtcg tcggatgtgc actgtatgaa gagcggttgg aaatcatgat tgcggatcat
     1441 ggcacgagct tcgactttga agagacaaaa aaacatattg gaccatatag cgacacatcg
     1501 gatgctcgtt tcctccgcga gggtggactt gggctttatt tgatggagac gctcatggat
     1561 gaagtgaaat ttcatcatga ggagggcgtg acagtcttta tgactaaata tctcggcaga
     1621 gagcggggcg aagaagatgt caaacgaaca gccaactcat aagccggata cgaaagagaa
     1681 agtattggag tggatcaggc aataccaaga gacgaatgac gatgaggcgc agacgaatct
     1741 tgtccttcat tatgaacggc tcgtccattc catcgcccgc aaatattcaa atgggaaacc
     1801 gtaccatgaa gatatcgtcc aagtcgggat gctcgggctt ctcggagcga tccgaaggta
     1861 tgacccggaa ctgggtcgca gcttcgaagc gtttgccgta ccgacgatcg tcggggaaat
     1921 caaacggttc ttgcgtgata aaacatgggc cgtccacgtg ccaagacgaa tcaaggaact
     1981 tggtccgaaa atcaaggccg cggtcgagac gttgacgatt gaaatgcaac gatcgccgct
     2041 cgtaagtgaa atagcggaat acttggatac tgatgaagaa ttggttcttg aagcgatgga
     2101 aatggggagg agctatcaag ccctttcgat ggaccatacg ctggaagccg attcggaagg
     2161 cggcactgtg accttgttcg atattattgg agaatcggat gacggatatg agaggacgga
     2221 ccatcgactt gtcgtggcca atgcgctcaa tgtgttgtct gaacgtgaaa agcagatcat
     2281 ccaatataca tatatcgagc agctaagcca aaaagaggct ggtgaacgtc tcggtatttc
     2341 gcaaatgcat gtctccagac tgcagcggaa agcgatcaaa aaacttcaag aggccattgt
     2401 ggcagctggt ggtgcatcgt agtggaagtg ttcaaaaatg atcatgtaga ggcgtacatc
     2461 tataacgcgg caaaacatgg caatatcgaa tcgggggata cgtattatgt ccattccgaa
     2521 gaggaatact tcatttgcgc aattgccgat ggacttggaa acggcgcagt tgcaagacaa
     2581 tccgcggaag tcatcccaca ggtcctcgag cattatcatc acgaatcgct cgatgaactg
     2641 cttaaccgct gcaatgaaca tatggttcag aagcgcgggg cggccgtagc gattgtccgt
     2701 gtcaactaca aagaaggcac gattgaatac agctgtgtcg gaaacgtcag gttgtatatt
     2761 ctacatggcc acgaaaagat gatctatcct cttccggtca tgggatattt atcaggacgc
     2821 ccgcaaaaaa tgaacactca aaaatacaag tacgaagtag gcgatctgtt tttccttcat
     2881 tcggacggca tcacgctgaa cagcccgaaa gcgagtttga agcagagctc caacgcctat
     2941 gatctataca acacgataaa aaaatcggcg caatccggcg atgacgccac attcatcgcc
     3001 ggcagcctgc ttcagtaaaa tgaagcaggt tttttatttg caagaatgga gtaagtacag
     3061 cgatcaatgg taaactgtag gaaagaaagg gtgggaatga atggcgaaca agttaataga
     3121 agcaactgca aaaagggcat cgatcagtac gaagcagacc gccaatgtca ttgctcttct
     3181 cgacgaaggc aatacggtcc ctt
//
LOCUS       DASULI010000220         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_276103, whole
            genome shotgun sequence.
ACCESSION   DASULI010000220 DASULI010000000
VERSION     DASULI010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276103"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1746
                     /locus_tag="VFX34_04790"
     CDS             100..1746
                     /locus_tag="VFX34_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEX5564256.1"
                     /translation="MNYDVIVVGAGLAGLVATSEVIKAGKRVLLLDQEPASSMGGQAW
                     WSFGGLFLVDSPEQRRLGIKDSKELAWQDWMGSAGFDRPNDEDHWAMKWAEAYVDFAA
                     NEKREWLHSLGVRFFPVVGWAERGGYLAEGHGNSVPRFHITWGTGPGIVKPFEEMVRE
                     GIAKGLVDYKPRHQVDELLTENGTVVGVKGSELEPSDAERGERSSRTVIGKFTYKAPA
                     VVVTSGGIGANFDIVRENWPARLGEPPKEMISGVPAHVDGRMLAITENAGGRIVNKDR
                     MWHYTEGIRNWSPIWPLHGIRILPGPSSIWLDAEGNRFPAPNFPGFDTLGTLQAIKET
                     GYDYSWFILTEKMIEKEFALSGSEQNPDLTGKSIKKVLSRVLPGPPAPVKAFMDHGED
                     FIVAETLEELVTGMNRVAGNDLINAEKIERQLLARDREMENKFTKDLQITALRGARNY
                     IGDKLIRVAKPHKILDPKNGPLIAVRLNILTRKTLGGLQTDLSGRVLNEEGTPIPGLY
                     AAGEVAGFGGGGVHGYRALEGTFLGGCLFSGRIVGRTLSE"
     gene            1810..>3027
                     /locus_tag="VFX34_04795"
     CDS             1810..>3027
                     /locus_tag="VFX34_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX5564257.1"
                     /translation="MTVQQFLEEQNRVIKDLHVTSASSSWMAATTGEDEWNQKTAEAQ
                     TAFNAYLSNPERFEQVKAYLGQDDLTAVQRRQLESLRTGMAGKQIPEADLKEMVERST
                     ELIGIFNTFRATIDGKPVSENDVRQILIKNNGNAEREEAWHASKQIGKEVEEKLLTLV
                     KKRNEVARSLGFENYHQMSFEHQELDRDEVFSIFQKLKELSDEPFREMKHEMDSELAE
                     RFGISIDELRPWHYADPFFQEAPPSKELDLDPFYDGKDLEQLTIATFESMGMDIKDML
                     ANSDLYPRDKKNQHAFCSDIDREGDVRVLCNNVPSDYWSVTMLHEFGHAVYFKYVDRQ
                     LPFLLRTCAHTLTTEAIAMLYERFGKNPAWLQQFLGLDKAQVEELTPLIEESQRRQVL
                     IACRWIMTFTFFER"
BASE COUNT          797 a          662 c          933 g          635 t
ORIGIN      
        1 ggaagaagta tacgttgctg ggtgtattga aaagaaggga ttggaaagac aagcggcgaa
       61 ataggcaatg tgggatttac tgacattggg ggatgggaaa tgaattatga cgtcattgta
      121 gtgggagccg gccttgccgg attggtggcg acttcggaag tgatcaaagc cggaaaaagg
      181 gtgcttttat tggatcaaga gccggcgtcg tccatgggcg ggcaggcgtg gtggtcgttt
      241 gggggattgt tcttagtcga ttcgccggag cagcggaggc ttggtataaa ggattccaag
      301 gaactcgcct ggcaggattg gatggggagc gcgggattcg accggcccaa cgacgaagat
      361 cattgggcaa tgaaatgggc ggaagcgtat gtggatttcg cggcgaatga aaagagggag
      421 tggctccatt cgcttggcgt ccggtttttc cctgtcgtcg gatgggcgga acgaggaggc
      481 tatcttgccg aagggcacgg caattcagtg ccgcgattcc atattacatg ggggacgggt
      541 ccggggatcg tgaagccgtt cgaagaaatg gtccgcgaag gaattgccaa agggctcgtc
      601 gactacaagc cgcgccatca ggtggatgaa ttgctgacag aaaacggaac ggttgtcggc
      661 gtgaaagggt ccgaactcga gccgagcgac gccgaacgtg gtgaacggag ctcccgaact
      721 gtcatcggga aatttacata caaagcaccc gcagtcgtcg tgacaagcgg cggaatcggt
      781 gcgaatttcg atatcgtccg ggaaaattgg ccggcgcgac tgggggagcc cccgaaagag
      841 atgatttcag gcgtcccggc gcacgtcgac gggcggatgc ttgcgataac ggaaaacgcg
      901 ggcgggcgta ttgtgaataa agatcggatg tggcattaca cggaaggcat acggaattgg
      961 agcccgatct ggccgcttca tggcatccgt attttgcccg gtccttcttc catttggctg
     1021 gacgcggaag gcaaccggtt cccggcaccg aatttcccgg gcttcgacac gttggggacg
     1081 ctgcaagcga taaaggaaac gggctatgac tactcttggt ttattctcac ggaaaagatg
     1141 atcgaaaagg aattcgcgtt gtcgggttct gagcagaatc cggatttgac agggaaaagc
     1201 attaagaagg tgctatcgag agtgcttccg ggtccaccgg ctcccgtgaa agcattcatg
     1261 gatcacggtg aagatttcat cgtagcggag acgcttgagg aattggtgac tggcatgaac
     1321 cgtgtcgcgg gaaatgacct aatcaatgcc gaaaaaatcg agcggcaatt gcttgcaagg
     1381 gaccgggaaa tggaaaacaa attcacgaag gacctgcaaa ttactgctct tcgcggtgca
     1441 aggaattaca ttggagacaa gttgatccgc gttgcgaagc cgcataaaat ccttgatcct
     1501 aaaaacggtc cgttaatcgc ggtaaggctc aatattttga cacggaaaac attgggcgga
     1561 cttcagactg atttgtcagg acgtgtgttg aatgaagaag gcacccctat accgggatta
     1621 tacgcagcag gggaagtcgc cggcttcggc ggcggtggcg tgcatggata ccgcgcgttg
     1681 gaagggactt tcttaggggg ctgcctgttc agcggacgta ttgtcggtcg aaccttgtcg
     1741 gaataaagaa ggagattcgc caggcaatct cgaatgggag gataacgaca gaaaaaaggg
     1801 gaagagaaga tgacggttca acaatttttg gaggaacaaa atagggttat caaagatttg
     1861 catgtgacga gcgctagcag ttcatggatg gcggcgacga ccggggaaga cgagtggaat
     1921 cagaagacag cggaggcgca gaccgcattt aacgcatatc tttcaaaccc ggaacgtttc
     1981 gaacaagtaa aggcgtatct cggccaagac gatttgacgg cagtgcagcg gcgccaactt
     2041 gagagccttc gtaccggcat ggcaggaaag cagatcccgg aagcggattt aaaggaaatg
     2101 gtggaacgtt caacggaatt gatcggcatt ttcaatacat tccgcgcaac aattgacggg
     2161 aaacctgtgt cggaaaacga cgttcggcaa attttgatta aaaacaatgg taacgctgaa
     2221 cgcgaagagg cgtggcacgc aagcaagcag atcgggaagg aagtcgagga aaagctgttg
     2281 accctcgtca aaaaacggaa cgaagtcgcg cgttcactcg gctttgaaaa ctatcaccaa
     2341 atgagcttcg agcatcagga attggaccgc gatgaagtct tttcgatctt ccaaaagctg
     2401 aaagagcttt ccgacgagcc gttccgtgaa atgaaacacg aaatggacag cgaactggca
     2461 gagcgcttcg gcatttcgat cgacgagctg cggccatggc attatgccga tccgtttttc
     2521 caagaggcgc cgccttccaa ggagctggac ttggatccgt tctatgacgg gaaagatctc
     2581 gagcaactga cgattgcaac gttcgaaagc atggggatgg acatcaagga tatgctcgcg
     2641 aacagcgatc tctatccgcg cgacaaaaag aaccagcatg cgttttgttc ggatatcgac
     2701 cgcgaaggcg acgtccgcgt tctctgcaat aatgtgccat ccgattattg gtccgtcacg
     2761 atgctgcatg agttcggcca tgcggtttat ttcaaatatg tcgaccggca gcttccattc
     2821 ctattaagga cttgcgcaca tacgctgacg acggaagcga tcgcaatgct ttatgagcgt
     2881 tttgggaaga atcccgcatg gctgcagcag tttttagggt tggataaagc ccaagtggaa
     2941 gaattgacgc cgctcatcga ggaatcacag cgacggcaag tgctcatcgc atgccgctgg
     3001 atcatgacat tcacattctt tgagcgg
//
LOCUS       DASULI010000221         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_832635, whole
            genome shotgun sequence.
ACCESSION   DASULI010000221 DASULI010000000
VERSION     DASULI010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832635"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /gene="fmt"
                     /locus_tag="VFX34_04800"
     CDS             complement(<1..650)
                     /gene="fmt"
                     /locus_tag="VFX34_04800"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5564258.1"
                     /translation="MTSIVCMGTPSFSVPILTMLHEEGYDIKAVVTQPDRPVGRKRIL
                     TPPPVKEEAVRLGIPVLQPEKLKGSPELEKILSLSPDLVVTAAFGQLLPKELLDAPPL
                     GCINVHASLLPKYRGGAPIHKAIIDGETETGVTIMYMVEKLDAGNIISQVTVPIEETD
                     HTGILFDKLSAAGTELLKKTLPSIVDRKNYSIPQDESLVSFARNISREQERIDWKQP"
     gene            complement(653..1156)
                     /gene="def"
                     /locus_tag="VFX34_04805"
     CDS             complement(653..1156)
                     /gene="def"
                     /locus_tag="VFX34_04805"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5564259.1"
                     /translation="MAVRKIVTHPSPILTQPCKEVTAFDDKLATLLDDMYDTMIANDG
                     IGLAAPQVGESIRVAIVDLGEGQEVIEMVNPVVVAIGGSEIEIEGCLSFPDLYGEVER
                     PYYVKIEAQERDGSIYELEAEDYEARAILHEIDHLNGVLFDSKIIRVVDPSEFEEVED
                     EDEGEEN"
     gene            complement(1181..>3226)
                     /gene="priA"
                     /locus_tag="VFX34_04810"
     CDS             complement(1181..>3226)
                     /gene="priA"
                     /locus_tag="VFX34_04810"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5564260.1"
                     /translation="VTDPALVQFLAGRKRVAMKEAIHPGLLETIKAYAKEGVLTVDTA
                     VSQKTDIRKVRSIVLASTETLASIADSLARNAKRQIELIEWMMQHAGETVLAKDLFEQ
                     TGTSPAALKALIEKGAATEKMVEVYREPDAPMMKDTAIPEQLTPEQEVVMETIQEVID
                     AEKQETFLLHGITGSGKTEIYLRTIQDVLNRGKEAIVLVPEISLTPQMTSRFKARFGP
                     LVAVMHSGLSAGEKYDEWRKIHRGEVKVVVGARSAIFSPFTNLGVIILDEEHESTYKQ
                     EDTPRYHARDVAIWRAAYAKCPVILGSATPSLESFARAQKGVYTLLTLSKRAKEQALP
                     AVTVVDMREELRKGNRSMFSIPLAEAIRKRIERGEQTVLFLNKRGYSSFVLCRDCGTV
                     VECPNCDISLTYHRAHERLKCHYCGHEERVPTTCPQCESEHIRFFGTGTQRAEEEIAR
                     LFPEARVLRMDVDTTRQKGAHERLLTAFSEGKADILLGTQMIAKGLDFPGITLVGVLA
                     ADTTLHLADFRAAEKTFQLLTQVGGRAGRHELPGEVFIQTYSPEHYAIELAKDQLYEP
                     FYNREMGTRKLFGYPPFYFITLVQFTHEDVLTVADYAERATGFLRENLSSDTVIIGPT
                     ASAISRVNNRYRYQCLIKYKKEPKLTEALKKLIRMYRTDWIKAGLTMSVDVEPASIT"
BASE COUNT          804 a          837 c          657 g          928 t
ORIGIN      
        1 ggttgcttcc aatcaatccg ttcctgctcc ctagaaatat tacgggcaaa cgaaacaaga
       61 gattcgtcct gtggaatact gtaattctta cggtcgacaa tagaaggaag cgttttcttc
      121 aacagctcgg tgcctgctgc agacaattta tcgaataaaa tgcctgtatg atctgtttcc
      181 tcaatcggca cagttacttg tgaaataata tttccggcat ccaatttctc gaccatgtac
      241 ataatggtta cacctgtttc cgtttcacca tcaatgattg ccttgtggat gggagcccct
      301 cctctgtact tcggtaggag ggacgcatgc acattaatgc aaccaagcgg tggcgcatct
      361 aaaagctcct tcggtaacag ctggccaaac gcagcagtca ccacaagatc aggagataga
      421 gaaaggattt tttctagttc aggagatccc ttaagctttt ctggttggag aaccggtatt
      481 ccgagacgaa cagcttcctc ttttaccggc ggcggtgtca agattcgctt tcttccgact
      541 ggacggtccg gctgtgtaac aaccgcttta atgtcatatc cttcttcatg aagcatagta
      601 agaatcggga ccgaaaagga aggtgtgccc atacacacaa tggatgtcat atttagttct
      661 cctccccttc atcctcgtct tcaacttctt caaattcaga tgggtcgaca acccgtatga
      721 ttttcgaatc aaacaagact ccattgagat gatcgatttc atgtagaatc gcacgcgctt
      781 cgtaatcttc cgcctctaat tcataaatcg agccgtcacg ctcctgcgct tcaatcttca
      841 catagtacgg gcgctccact tcaccataca aatctggaaa gcttaggcac ccttctattt
      901 caatttccga tccgccaatt gcgactacga ccggattgac catttcaatt acctcttgcc
      961 cttcgcctaa atctacaatt gcaacacgaa tcgactcgcc aacttgtggt gctgcgagcc
     1021 caatcccatc attcgcaatc atcgtgtcgt acatatcatc caaaagtgta gctagcttgt
     1081 catcaaacgc tgtgacttct ttgcatggtt gcgtaagaat tggtgaagga tgcgtaacaa
     1141 tttttcgtac tgccaaaaca attcctcttt tctatgttgt tcacgtaata gaagccggtt
     1201 ctacgtcgac tgacatcgtc aagccagcct tgatccaatc tgtccggtac atccggatca
     1261 gctttttcag tgcttctgta agcttcggtt cttttttgta ttttatcaga cattgatagc
     1321 gatatctatt gttcacacgg cttatagccg aagctgtcgg accgatgata acggtgtccg
     1381 atgataggtt ttcccgaaga aaaccagtcg ctcgttcggc ataatccgca acggtcaata
     1441 cgtcctcatg tgtaaattgc acaagagtta taaaataaaa tggcggatag ccaaacagtt
     1501 tcctcgtccc catttcccga ttataaaatg gctcatacag ctgatctttt gcaagttcaa
     1561 tggcataatg ctcaggagaa tacgtttgaa taaacacttc acctggaagc tcatgccggc
     1621 ctgcacggcc accgacttgc gttaggagct ggaatgtctt ctctgccgcc cggaaatcgg
     1681 ccaaatgaag tgttgtatcc gctgcaagta ctccaacaag cgtaatccct gggaaatcaa
     1741 gcccttttgc aatcatctgg gttcccaaca agatatcggc ctttccttca ctaaatgcag
     1801 ttaaaagtcg ttcatgtgca cctttttgcc gtgtggtgtc aacatccata cgaaggacac
     1861 gcgcctccgg aaacagtctt gcaatttctt cttcggctct ttgtgtccct gtaccgaaaa
     1921 atcgaatgtg ttcactttca cattgaggac atgttgtggg aaccctttcc tcatgcccac
     1981 aatagtggca tttcaatcgc tcatgggccc ggtgatatgt taaggaaata tcgcagttcg
     2041 gacattccac aactgttcca cagtctcggc acaagacaaa tgaagaataa ccgcgtttat
     2101 tcaaaaagag gaccgtttgc tctccccgtt cgattcgttt tcggattgct tctgccaatg
     2161 gaattgaaaa catggaacgg ttgccttttc gtaactcttc tcgcatatcc acaacagtta
     2221 ccgctggaag tgcctgttcc ttagcacgct tggagagtgt caataatgtg tagacacctt
     2281 tttgagcacg cgcaaatgac tccagggacg gagtggcact gcctaaaatc acaggacatt
     2341 ttgcataggc tgcacgccaa attgccacat cccttgcgtg ataccgggga gtgtcctcct
     2401 gtttatacgt ggactcatgt tcttcatcca aaataatgac acctagattt gtaaatgggg
     2461 agaaaattgc agaccttgca cctacaacga ctttcacttc tcccctatgg attttcctcc
     2521 actcgtcata cttttcacca gccgaaaggc cagagtgcat gacagcaacg agtggaccaa
     2581 accttgcttt aaatcgagat gtcatttgtg gcgtcaatga aatctctggt acgagcacaa
     2641 tcgcttcttt gccacggttt aaaacatctt gaatcgtccg caagtaaatt tccgtcttcc
     2701 cacttccggt tatcccatgt agaagaaacg tctcctgttt ttcagcatca attacctctt
     2761 gaatcgtctc catgacaact tcttgttcag gcgtcaattg ctccggaatc gcagtatcct
     2821 tcatcatggg agcatccggt tcccggtaca cttccaccat cttttcagtg gccgcgcctt
     2881 tttcaatcaa ggccttcaat gctgcaggtg atgtcccggt ttgttcgaat agatccttcg
     2941 ccaacacggt ttccccggca tgctgcatca tccattctat taattcgatt tgccgttttg
     3001 catttcgggc caatgaatct gcaatacttg caagcgtttc agttgatgct agaacaattg
     3061 accgtacttt ccggatatct gttttctgac tcactgcagt atcgactgtg aggacgcctt
     3121 ctttcgcgta cgccttaatt gtctctaaca gtcccgggtg aattgcttcc ttcattgcca
     3181 cacgttttcg accagccaaa aactgtacca gcgccgggtc tgtcac
//
LOCUS       DASULI010000222         5895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1110080,
            whole genome shotgun sequence.
ACCESSION   DASULI010000222 DASULI010000000
VERSION     DASULI010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110080"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFX34_04815"
     CDS             complement(<1..207)
                     /locus_tag="VFX34_04815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5564261.1"
                     /translation="MYNSLWLQTAYRRDSYPTLDKDVSCDVCIIGGGLSGIANAYFLA
                     KEGKDVVLLEKDEILCGATGNSTGK"
     gene            310..1017
                     /locus_tag="VFX34_04820"
     CDS             310..1017
                     /locus_tag="VFX34_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX5564262.1"
                     /translation="MKIEIWSDYVCPFCYIGKRRLEEALQETNLGDQVEVEFKAFQLD
                     PNTAVGAGGSVPQYLAEKYSISMDEAKNMLGNVAAQAKTVGLDFDIDNMVGANTFDAH
                     RLAKLAESEGKGAVVSERLLQGHFVNGEAVDNEDTLLAIAEGAGISKERIKEVLQSDE
                     FSNDVKRDIAEAGQLGVQGVPFFVINRKYAISGAQPVEAFTEALKKVAAEEGIQPKLK
                     VLGIDGQGVCADDNCDI"
     gene            complement(1314..1700)
                     /locus_tag="VFX34_04825"
     CDS             complement(1314..1700)
                     /locus_tag="VFX34_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HEX5564263.1"
                     /translation="MSVNEIGEFIGALLILTGGIASVISAFGLIRLPDIYTRSHAATK
                     SSTLAVLLTLSGTFLYFLFREQFVSVRVLLGIVFVFLTAPVAGHLLVRAAYRSKVKLA
                     DISTEDELYEVLYGKENENDGSSDKS"
     gene            complement(1678..1962)
                     /locus_tag="VFX34_04830"
     CDS             complement(1678..1962)
                     /locus_tag="VFX34_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit F1"
                     /protein_id="HEX5564264.1"
                     /translation="MIETMITISLVLFSITISIAVLRIILGPSMPDRVIALDMIGVNL
                     IAMIAVVSILLKTKAFLEVILILGILSFIGTIAFSKAIERGVIIERKRDR"
     gene            complement(1959..2441)
                     /locus_tag="VFX34_04835"
     CDS             complement(1959..2441)
                     /locus_tag="VFX34_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX5564265.1"
                     /translation="MPAQLLLNLFIAFLWMTLMDEDELRFTTFFAGFLVGIGIIFFMH
                     RFFGTQFYLRRLFSSFKLLLIFISELTQSSIVVLRQILSPELKIKPGIFKYETVLKSD
                     VEVTMLSLLLTLTPGSVVMEVSPEGNTLYIHAMDVEQSRAGLIKQLKNFEKAIMEVTR
                     "
     gene            complement(2465..3949)
                     /locus_tag="VFX34_04840"
     CDS             complement(2465..3949)
                     /locus_tag="VFX34_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HEX5564266.1"
                     /translation="MNNIVAMPMVIPLLTGIILVFLRHYTKTQRFLSILSMIATVAVS
                     LVILHRIQQEGILRLDFGGWLPPYGILFVADSFAMLLVVTASLVTGILLIYAFSSIGK
                     QYEHMFFYPFVFFLVAGVNGSFLTGDLFNLFVCFEVMLLSSYVLITMGGRKIQLIESI
                     KYVTINVLSSWFFLMGIAYLYGTIGTLNMAHLSIRIAEVGQGPLLTVISLIFLIVFSL
                     KAGLLLYFWLPGSYSAPPTVIAALFGALLTKVGIYAMFRVFTLIFYHEPVITHTAIGV
                     MAILTMIGGSVGALAYNDIRKIVSYNVVIAVGFILAGLAISSEIAFQGAIFYLIHDMV
                     VKALLFLLAGTMIYLTGKAKIEEMSGLIRNYPMLGWLFFITMLSLAGVPPLSGFIGKV
                     FIGQGAIETGSFILLTVAFLSGLFVLYSLLRIFLNCFWGETIISEDDDVPLKKGMLVP
                     CILFAVLTVIIGIGVEGLAPYVSDAARTLMDPEIYIDAVLQGNR"
     gene            complement(3942..4280)
                     /locus_tag="VFX34_04845"
     CDS             complement(3942..4280)
                     /locus_tag="VFX34_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit C"
                     /protein_id="HEX5564267.1"
                     /translation="METLITILVGFLVMIAVYLLLSRNLIRVILGTAVLSHAAHLLLM
                     TMGGLKKGSAPLLSEDAESYTDALPQALILTAIVISFAVTAFLLVLAYRTYKKSGTDN
                     LQELRGFEDE"
     gene            complement(4284..4706)
                     /locus_tag="VFX34_04850"
     CDS             complement(4284..4706)
                     /locus_tag="VFX34_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011067505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HEX5564268.1"
                     /translation="MKINDVILQTVTRIVVFIILTFGVELFLSGHNNPGGGFIGGLVL
                     SSAFVLLYLVNDIETVHKGIPFDFKKIAALGAFLAVGTGIGSVLFGKDFLTQASRHFN
                     LPVFGETELSTVIVFEAGVALTVVGVVVTIISTISEDV"
     gene            complement(4693..>5895)
                     /gene="mbhE"
                     /locus_tag="VFX34_04855"
     CDS             complement(4693..>5895)
                     /gene="mbhE"
                     /locus_tag="VFX34_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HEX5564269.1"
                     /translation="FLSKEMFFESLVNARDLNLFEVGTMGTLFLIVAWLASIFTFVYS
                     MIIVFQTFFGEFQPERIEKPSREAPFGMLIAPSILALLVVGIFFFPNVIGDYLLRPAL
                     EAIYPSVEGVERLVPKISAWHGFNTALMMTVGVIVLGIILYRFMRYWKGIYSIAPLNW
                     NLDAAFNATLILLEKLSHFFTRLYMKGYLKDYLVYIFTFFILAVGGAIVYTGSYSFDF
                     SDDSPITTNEYLIVLAMTVAGISILFAKSRMTAIILNGVLGYSIAFFFVVFRAPDLAL
                     TQAVVETVTTALFLLCFYFLPEWKKERSPISTKLANGLIAFSVGTIVTVLALTVQGNK
                     MFESISVYFEDAYNLAGGRNIVNTILGDFRAFDTMLEVVVLFIAGIGVFTLIKLKVRK
                     EEKDIEDQ"
BASE COUNT         1780 a         1427 c         1392 g         1296 t
ORIGIN      
        1 tttgcctgtt gaattgcctg tagccccgca taggatttca tccttctcaa gcaggacgac
       61 atcttttcct tctttcgcca gaaaatacgc gtttgcaatg ccgctcaaac ctccgccgat
      121 aatgcacaca tcgcatgaaa cgtctttatc cagagtgggg taggaatccc gccgatacgc
      181 ggtctggagc catagtgaat tgtacatgtc atgccctcct attggtcttc gataacgata
      241 gcatcaggca tattcattgt tgccatttta attgaaatct aaaatggaag atgaaaggtg
      301 gttaaggtcg tgaaaataga aatctggtca gactatgttt gcccgttttg ttacatcggg
      361 aaacggagat tggaagaagc gttacaggaa acaaacttgg gagatcaagt ggaagtggag
      421 ttcaaagcgt tccaattgga tccgaacacg gctgtaggcg cgggaggctc cgtaccacaa
      481 tacttggcgg agaaatacag catcagcatg gatgaagcga agaacatgct tgggaatgtt
      541 gcagcgcagg cgaaaacagt cggactcgac tttgacatcg acaacatggt cggagcaaac
      601 acattcgacg cgcaccgact ggctaaactg gccgaatcgg aaggcaaggg ggctgtagta
      661 tcggaacgtc ttctgcaagg ccacttcgta aatggagaag cagtcgacaa tgaagatacg
      721 ttgctcgcta ttgcggaagg ggcagggatt tcgaaagaac gtataaaaga ggtgcttcag
      781 tcagatgaat tttcaaatga cgtgaaaagg gatatcgcag aagctggaca actcggcgtt
      841 caaggcgtcc cattcttcgt catcaaccgc aaatacgcca tttcaggggc acagcctgtt
      901 gaagcattta cagaagcgct gaaaaaagtg gcagcagagg aaggtatcca acctaagttg
      961 aaagtgttag gtatcgatgg tcaaggcgtg tgcgcagatg acaattgcga catttgatta
     1021 atgtaacggc tttctatgat taaaggtgtc ccattgattg aagcgaaagc ggcgactcct
     1081 gggggattag cgaacgccgt cacgaagaac ggcgtttgct agcacaagcg cagcgttgcg
     1141 agcgatgttt cctgcgacct taagggagca ggatgcctta atttctgcaa ggaacgcaga
     1201 aattaaggca aatcgaaccc ttcgctgttc gattggctca acgcccgccc cccggaaagc
     1261 gtccgcctgt agcggaaatc aacatcattg aatagccaat aaaaaacgac ggtctagctt
     1321 ttatcgctag aaccgtcatt ttcgttttcc ttcccatata aaacttcata caactcatct
     1381 tccgttgaaa tatccgcaag cttcaccttc gagcggtacg ccgccctgac aagcaagtga
     1441 cctgccaccg gtgcagtgag gaagacgaaa acgataccga ggagcacccg cacactgaca
     1501 aactgctccc ggaacaagaa atagagaaaa gtgcccgata acgtcagcaa aactgccagg
     1561 gtcgagctct tcgtcgcagc gtgcgacctt gtgtagatat ccggcaagcg gataagcccg
     1621 aaagcactaa tcacactcgc aatcccgccc gtcaaaatca acagggcccc tatgaattca
     1681 ccgatctcgt ttacgctcaa tgatgacccc cctctcaatg gctttcgaaa aggcgatcgt
     1741 tccgatgaag gacaggatcc ctaaaatcaa aatgacctcc aaaaaagctt tcgtcttcaa
     1801 gaggatcgag acaacagcaa tcatcgcaat caaattgaca ccgatcatat ccagtgcgat
     1861 gacacggtcc ggcatggagg ggccgaggat gatccgtaac acggcgatgg aaattgtgat
     1921 ggaaaacagg acgagtgaaa tcgttatcat tgtttcaatc atcgtgtcac ctccataatc
     1981 gctttttcaa aattcttcag ttgtttgatc aaaccggcac gggattgctc gacatccatc
     2041 gcatggatat aaagcgtatt gccttcaggg gacacttcca taacgacaga ccccggcgtc
     2101 aaggtgagga gaagtgacag catcgtcact tccacatcac ttttcaaaac ggtttcgtat
     2161 ttaaagatgc cgggcttgat tttcaattcg ggacttaaaa tctgccgcaa tacgacgatg
     2221 ctggactggg tcaactccga gatgaaaatc aacagcaatt taaaggaaga aaataaccgg
     2281 cgcaaataaa attgcgtgcc gaaaaagcgg tgcatgaaaa agatgattcc gattccgaca
     2341 aggaaaccgg cgaagaaggt cgtgaacctc aattcatctt catccatcaa agtcatccat
     2401 aaaaaagcga tgaacagatt taataataac tgggctggca ctcatcttca cctcaacttt
     2461 ccacttaacg attcccttgt aaaacagcat caatatagat ttccgggtcc attagcgtcc
     2521 gagctgcatc gctgacataa ggagcgaggc cttcgacgcc gattccgatt ataaccgtca
     2581 agacggcaaa taggatgcaa ggaacgagca tccctttttt caatggaaca tcgtcgtcct
     2641 cactgatgat cgtctcaccc caaaaacaat tcaggaagat gcgcaacagc gaatacagga
     2701 cgaaaaggcc ggataagaag gctacggtca aaaggatgaa ggagcccgtt tcgattgccc
     2761 cctggccgat gaacaccttg ccgatgaacc cgcttaacgg cggtactccc gccaatgaca
     2821 gcatcgtgat gaagaagagc cagccgagca tcggatagtt ccggatcaaa ccgctcatct
     2881 cttcaatctt cgctttcccg gtcaaataga tcatcgtacc cgcaagaagg aacagcagag
     2941 ccttgacgac catatcgtgg atcaaataaa agattgcccc ctggaatgcg atttccgatg
     3001 atattgcaag tcctgccaaa atgaaaccga cagcaatgac aacgttatac gaaacgattt
     3061 ttcggatgtc gttataagca agcgctccga cgcttccgcc gatcatcgta agaatcgcca
     3121 tgacaccgat tgcggtatgc gtaatgaccg gttcatggta aaagatgagg gtgaagacgc
     3181 ggaacatcgc gtaaatcccc actttcgtaa gcaaggcgcc gaagagggcc gcaatgacgg
     3241 tcggtggtgc gctgtacgat cccggcagcc agaaatacag cagcagccct gccttcaaac
     3301 tgaagacgat caaaaagatg aggctgatca ccgtcagcag cgggccttgt ccgacttcag
     3361 caattctgat cgataaatgg gccatgttca acgtgccgat cgttccgtat aaatacgcga
     3421 tccccattaa gaagaaccat gaggaaagga cattgatcgt gacatatttg atggactcga
     3481 tcagttggat cttcctgcct cccattgtaa tgaggacata ggaggaaagg agcatcactt
     3541 cgaaacagac gaataggttg aacaaatccc ctgtcaggaa tgaaccgttg actcctgcaa
     3601 cgaggaagaa gacgaacggg taaaaaaaca tatgctcata ttgctttcca atcgatgaaa
     3661 aagcgtaaat gagaagaatg cctgttacga gcgaagctgt cacgacgagc agcatggcaa
     3721 aagaatctgc aacgaataaa atgccgtaag gtggaagcca gcccccgaaa tcgagccgta
     3781 gaatcccttc ctgctggatt ctgtgtaaaa tgacgaggct gacagctact gtcgcgatca
     3841 tcgacaggat gctgagaaaa cgctgcgtct tcgtgtaatg acgtaaaaaa actaggatga
     3901 tccccgttag taaagggatg accattggca tggcgactat gttattcatc ttcgaacccc
     3961 ctcaactctt ggaggttatc cgttccggac tttttgtatg tacgataggc aagtacaagc
     4021 aggaatgcgg tgacggcgaa actgattacg atcgctgtca ggatcagtgc ctgtggcaag
     4081 gcatccgtat agctttccgc atcctcgctg agcagcggtg cactgccttt ctttaatcct
     4141 cccatagtca tcaatagcag atgcgccgca tgcgatagga cggcagtccc taaaatcacg
     4201 cgaatcaaat tacgggacag cagcaaatag acagcaatca ttacaagaaa gccgacaagt
     4261 attgtaatta acgtttccat ctgctacaca tcctcactaa tggttgaaat aattgtcacg
     4321 actacaccga caaccgtcaa tgccaccccc gcttcgaata cgatcactgt cgaaagttcg
     4381 gtttcgccga aaacgggtaa attgaaatgg cgggaagcct gagtcaggaa atccttgccg
     4441 aacagtaccg agcctatgcc ggtaccgacg gcaagaaacg cccctagagc cgcaattttc
     4501 ttaaagtcga acggaatgcc tttgtgcacc gtttcgatat cgttaactaa gtaaagcaat
     4561 acgaatgcgg aggaaaggac gagaccgccg atgaaaccgc cgccgggatt gttatgccct
     4621 gacaggaata gctcgacgcc gaacgtcaat atgatgaaaa caacgattct cgtcacggtt
     4681 tgtaagatca catcattgat cttcaatgtc cttttcctcc tttcgcactt tcaatttaat
     4741 aagcgtgaac acgccgatcc cggctatgaa aagtacaaca acttccagca ttgtatcgaa
     4801 cgcacggaaa tctcccaata tggtattgac aatatttctg cccccggcca ggttgtatgc
     4861 atcctcaaag taaaccgaaa tggattcgaa cattttattc ccctgaacag tgagggcgag
     4921 taccgtcaca atcgtaccga cggaaaaagc gatcaatcca ttcgccaatt tcgtactgat
     4981 cggcgaacgc tccttcttcc attccggcaa aaagtagaaa caaagaagga atagggcggt
     5041 tgtcactgtt tcaacaacag cttgtgtcag cgccaaatcc ggtgcccgga agacgacgaa
     5101 gaagaaagca atggaataac cgagtacgcc atttagaatg attgccgtca tccgggattt
     5161 tgcgaagagg attgaaatcc ccgcaaccgt catggcaagc acaatcagat attcattcgt
     5221 cgtgatcggt gagtcatccg aaaagtcgaa tgaataggaa cctgtataaa caatcgctcc
     5281 accgaccgcc aaaatgaaga aagtgaaaat atatacgaga taatccttca aatacccttt
     5341 catatacagg cgggtaaaga agtgggacag cttttcaagc aggattaaag tggcattgaa
     5401 agcagcatcc agattccagt tcaatggtgc aatcgaataa atgcctttcc aataacgcat
     5461 gaatctgtaa aggatgattc ccagaacgat gacaccgacc gtcatcatca aggcggtatt
     5521 gaaaccgtgc catgcggaaa ttttcggtac caatctctca acaccttcaa ctgaagggta
     5581 gatggcttcc aatgcggggc gcagcaaata gtcaccgatg acattcggaa agaaaaagat
     5641 cccgacgact agaagagcca aaatggaagg cgcgataagc attccgaatg gagcttccct
     5701 ggatggtttc tcgatccgct ccggttggaa ttccccgaaa aacgtttgaa aaacgataat
     5761 catgctatat acaaaagtga agatgctcgc gagccaggcg acaatgagga agagcgtccc
     5821 catcgtgccc acttcgaata gattcaaatc acgcgcattc accaacgact caaaaaacat
     5881 ttccttgctt aggaa
//
LOCUS       DASULI010000223         7978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_822368, whole
            genome shotgun sequence.
ACCESSION   DASULI010000223 DASULI010000000
VERSION     DASULI010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822368"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /locus_tag="VFX34_04860"
     CDS             complement(<1..1934)
                     /locus_tag="VFX34_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX5564270.1"
                     /translation="MFDSLLFDLMLVILIGMLSQWIAWKYRMPAIVVMALAGLLVGPV
                     FGLINPQESMGDLFGPIITFAVAIILFEGSLNLDVREIKGFSKPVGRIVTIGAFIAWI
                     AGSLAAHYLAGLSWEVAFIIGGLFIVTGPTVILPLLRQARLKPRPAAILKWEGIVVDP
                     FGALLAVFAFEFIKFINAEVTLKSLLLFFAASAFAVLLGWFAALFIGNSFERGSIPEF
                     LKAPILFVVVLFTFVFADEIMHETGLLAVTAMGMKMANMRLTTLADVRHFKENISVLL
                     ISGIFVMLTASLDPKILIEIFNPAIMTYVAAMLFVVRPLSIWISTIGTELNVREKLLI
                     GWIAPRGIVALTVSGYFAGILLENGYEDAELLTALTFALVLSTVVLHGFSIGFIAKKL
                     NLTTTDESGVAIIGGTRFAAEMAQSIKDTGNEVLLIDQSWAALADARRRGLDSYVGDI
                     LSEQIEYHLDLTPYRYMLAMTKMDLYNAHVCADFEPDLGRDHLFQTAFHVGKDVEAFT
                     VVGGQTLFSPSISIYSLEERMHAGHVIRKTLITKQYSYTQYLRERDDKSILLYILRAD
                     GSIEFYSPNVELQANAGDAVIALSSPAKTIERVKERLEGENGDSEIPYEKLEELFTED
                     PSEGKRDIPGEAPKSNGDSV"
     gene            2071..3126
                     /locus_tag="VFX34_04865"
     CDS             2071..3126
                     /locus_tag="VFX34_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564271.1"
                     /translation="MIPIENSKKKTVAFYIARSDGKGSYPARRASELARDIVKEVNVL
                     FIVGENSPSPPDGFKSITIGKKANLIQTLTLIRPDLLIRDTGPTVSEEVEKIRSIVPS
                     ILHFDDFGDGGRQADFVLQTLIQESIDEHAENIVVDRQLFMADPSMRSFEKSGLRKSP
                     PGPLPHLVISFGEEDEGNLTYRALRHVSQLQIPLKVTVLVGERYPHDVSTLRMMALGR
                     RNTIVQQPPYSVPEVYATADVILCASGYMPYEVAVMGIPCVVLSQNDFELGLAFPKEQ
                     HGFVHLGLGRKVKQSNLLNAIMEPLLHEPLRKRSIERQLSLGLGDGKDKVIETIQYLL
                     EHPKHGAGGKAMSDMLH"
     gene            3151..3339
                     /locus_tag="VFX34_04870"
     CDS             3151..3339
                     /locus_tag="VFX34_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SE1832 family protein"
                     /protein_id="HEX5564272.1"
                     /translation="MNKRQIESAIAELKMDYISLQGDVEKLESTGHADSVHKAEQRLA
                     AMEERLAELNKQLADISE"
     gene            complement(3517..4968)
                     /locus_tag="VFX34_04875"
     CDS             complement(3517..4968)
                     /locus_tag="VFX34_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX5564273.1"
                     /translation="MKKMKKWVQPKWIVLMAILLFLFLYVGVITWHTYKPLPEGVNYE
                     GDIHWTDDVEMLTDLTYAQNKKGDGMVHELNIFDEIYNMIDEAEEFIVLDFFLMDHYT
                     DENIDFPKIAETLTEKLIQKKKENPEMKIIYITDPLNTGYGSYESKWFSRLEDAGIEV
                     VYSDLDKLRDSTPIYSGLYRTIFRWVNFEKEGWIANAMSSKAPKMTLRSYLILLNVKA
                     NHRKTVVTDKSAIVTSGNPHDASGLHGNVALKVKGTVLNDILEAEEAVVRYTNGGSLP
                     RADVEKEHDGDYAVQYLTEKKIHDALLGDIAAAQEGDTIRMGMFFIAMPDIVQAIVDA
                     SNRGVHVQMILDPNENSFGNEKSGLPNRPVLQKMMEETDGKLDVRWYNTVVGQYHTKL
                     VVIQTKQETYITNGSANLTDRTLNNYNLEANLRVIAPNDSELSKEIDAYFDRLWNNDD
                     ALYTLDFEEYQDDFTFFQRGIYRLQELLKLTTY"
     gene            complement(5088..5633)
                     /locus_tag="VFX34_04880"
     CDS             complement(5088..5633)
                     /locus_tag="VFX34_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5564274.1"
                     /translation="MKIDLTELYDEYGRYIYHLCLKLTRNKEEAEDLMQDVWVKVVRY
                     SGKMDSVDRMKAWLTTICMNTFRDRYRKDVRRSKHVMHQPETLDVPILDLVPSDNPTP
                     GEMIEQNDIQLMVQQKIGQLDGIYRTTIEYFYVYQYSLIEIADLMKVSIGTVKSRLFR
                     AKKYLKELMMDDTTVREYVTA"
     gene            complement(6082..7335)
                     /locus_tag="VFX34_04885"
     CDS             complement(6082..7335)
                     /locus_tag="VFX34_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX5564275.1"
                     /translation="MFIALGFFLLMSFFLSGSETALTAVSRMKVQLRAEQGDPKSIKL
                     RDLISKPDKMITAILIGNNVANIMMPTIVTMIAIDRGLKVGVMTGILTVVLIVFGEVL
                     PKTIAATFADRIAYVVAPTIAVFVKLLTPLTILLSLFTNIFIRIISKGAVTEATLTKE
                     ELRSMVDIASTEGTFEQEESLRLKGVLDFPEKDVSDVMETHRTDVIGLPIESTYEEVR
                     DTILEYFYTRYPIYEESIDRIVGIFYSKTLIEWSMNPEKKLADLMDEEPLFVVQTASV
                     EKVFKLMLAHKKHMAIVLDEYGGTLGIVTHEDIIEEMIGQDIEDETDFDEEVLVYEKN
                     EELLICHGRLEIVDAIDLLDVELETEHETIGGFVMQELGHVPDEGERFTYENLYFEIE
                     EMDRSRIIKMKITKRHDQENDRPAE"
     gene            7502..>7978
                     /locus_tag="VFX34_04890"
     CDS             7502..>7978
                     /locus_tag="VFX34_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5564276.1"
                     /translation="MSNLEKLDAYFTSKREEHLEQLKDFLRIPSISALSEHKSDMQKA
                     AEWLKDSFRKAGLENMSIDETEGHPVVYGDWLHAEGKPTILFDGHYDVQPVDPLNLWE
                     SGPFDPEVRDNKLYARGASDDKGQVFMHIKAVEALMNEDGSLPVNVKFIIEGEEEIG"
BASE COUNT         2034 a         2076 c         1831 g         2037 t
ORIGIN      
        1 ttaacagagt ccccgttcga cttcggcgcc tctcccggaa tgtcccgctt gccttcggaa
       61 ggatcctcgg tgaacaattc ttccaatttt tcatatggaa tctcagagtc gccattttcc
      121 ccttccaacc gctccttcac acgttcgatc gtcttcgcag gcgaagatag cgcaataacc
      181 gcatctcccg cattcgcctg cagttcaacg ttcggcgagt agaactcaat ggagccgtca
      241 gcccgcagga tatagagcaa gattgattta tcatcccgtt cgcgcaaata ttgcgtgtag
      301 ctatattgct tcgtaatgag cgtcttccgg atgacatgcc cggcgtgcat ccgctcctcc
      361 agactgtaga tcgagatgga tggggaaaat aacgtctgcc cgcctacgac cgtgaatgcc
      421 tcgacatctt ttccgacatg gaaagccgtc tggaataaat gatcacgccc cagatccggc
      481 tcaaagtccg cacaaacatg tgcattgtac agatccatct ttgtcatcgc caacatatac
      541 ctgtaaggcg tcagatcaag atggtattcg atctgctccg acagaatatc ccctacataa
      601 ctatccagcc cgcgccttct cgcatctgca agtgcagccc atgactggtc gatcagcagg
      661 acctcgttcc ctgtgtcttt gatcgactgc gccatttcag ccgcaaaacg cgtgccgccg
      721 atgatggcga cgcccgattc gtccgtcgtc gtcaaattca gctttttggc aatgaacccg
      781 attgaaaaac cgtgcaggac aaccgtcgaa agaacgagcg caaacgtcaa cgcagtaagc
      841 agctccgcgt cttcatatcc gttctccagc aaaatgcctg caaaataacc agatacagtc
      901 agcgccacga tgccccgcgg cgcaatccag ccgatcagca gcttttcacg gacattcaac
      961 tccgtcccga ttgtggaaat ccatatcgac aacggccgga cgacaaaaag catcgcagcg
     1021 acatacgtca tgattgcagg attaaaaatt tcaatcaaga ttttcggatc aagtgatgct
     1081 gtgagcatga cgaatatgcc cgagatgagc aatacggaaa tattttcctt gaaatgacgc
     1141 acatcggcca aggtcgtcaa gcgcatattc gccatcttca tccccattgc cgtgacagcc
     1201 aataaacccg tctcatgcat gatttcatct gcaaatacga atgtgaacag cacgacgacg
     1261 aaaaggattg gtgccttcaa aaactcgggg atgcttcccc gttcgaatga attcccgatg
     1321 aacaatgccg caaaccaccc gagaaggaca gcaaacgcgg aagctgcgaa aaacagcaag
     1381 agcgacttca acgtcacttc ggcattaatg aatttaatga attcaaatgc aaagacagcg
     1441 agcagcgccc cgaacggatc gacgacaatt ccttcccatt tcaagattgc cgcaggccgt
     1501 ggcttcaacc tcgcctgtct caataaaggc aaaatcactg ttgggccggt tacgatgaac
     1561 agccctccga tgatgaaagc gacttcccat gaaaggcccg ccaaataatg cgccgcgagc
     1621 gaaccggcaa tccaggcgat gaaagccccg atcgtgacaa tcctcccgac cggcttgctg
     1681 aatcctttta tttctctcac atccaaattc aagcttcctt caaaaaggat gatcgcgaca
     1741 gcaaatgtaa taatcgggcc gaatagatcc cccatgcttt cctgcgggtt gatcaatccg
     1801 aataccggtc cgacgagcaa tcctgcaagc gccatgacga cgattgcagg catcctatat
     1861 ttccacgcaa tccattggga aagcatcccg atcagaatga caagcatcag gtcaaataat
     1921 aaagaatcga acaacggaaa caccccgttc cccttcagaa taaatgctag tttaccacat
     1981 ttcctttttc attcaacgta tttgtacata actcgttttt tccaaacata gggtgaaaag
     2041 gagagtagtt tatacacagg aatagaaagg gtgattccaa tagaaaactc aaagaagaaa
     2101 actgttgcat tttacattgc gcggtcggac ggaaaaggtt cgtaccccgc aaggagagca
     2161 tctgagctcg ctcgggacat cgtaaaagaa gtgaacgtcc tattcatcgt cggtgaaaac
     2221 tccccttcac ctccggatgg cttcaaatcg atcacgatcg gtaaaaaagc caatctcata
     2281 cagacattga ctctcatccg tcctgacctc ctcattcgcg atacaggccc tacggtttcc
     2341 gaggaagtgg aaaagatccg cagcatcgtc ccttccattt tgcatttcga tgatttcggc
     2401 gatggaggca gacaagcgga tttcgtcttg cagaccctca tccaggaatc aatcgacgag
     2461 catgctgaga atattgtcgt cgatcggcag ctattcatgg ccgacccatc gatgcggtca
     2521 tttgaaaaat ccggtttgcg gaaatccccc ccgggtcccc ttccccatct cgtcatttca
     2581 ttcggggaag aagatgaagg gaatttgacg taccgcgcac tccggcatgt ttcccagcta
     2641 cagatcccgt tgaaagtgac cgtccttgtc ggggagcgct acccgcatga cgtcagcacg
     2701 ctccggatga tggcgctcgg aagacggaat acaatcgtcc agcagccgcc atacagcgtg
     2761 ccggaggtat atgcgacagc cgatgtcatt ttatgcgcat cgggctatat gccatacgaa
     2821 gtcgccgtca tgggaatccc gtgcgtcgtg ctatcccaga atgatttcga gctgggactt
     2881 gcatttccga aggaacagca cggcttcgtc catctcgggc tcggaaggaa agtgaagcag
     2941 tcgaatctgc tgaatgcgat catggagccg ctgctgcatg aaccgttgag aaaacgttca
     3001 atcgaacgcc aactatcgct cggactcggg gacgggaagg acaaagtcat cgagacgatc
     3061 cagtatttat tggaacaccc gaaacacgga gcgggcggaa aagcgatgtc ggatatgcta
     3121 cactaaggga aaatgattgg agttttccat atgaataaac gacagatcga atcggcgatt
     3181 gcagaattga aaatggatta catcagcttg cagggcgatg tcgagaaatt ggaatcgaca
     3241 ggccacgcgg actcggtcca taaagcggag caacggcttg ccgcgatgga agaaagactg
     3301 gctgaactga acaaacagct tgcagatatt tccgaatgaa aaaaaggctg ccgacgaacg
     3361 ggattcctgt tcgtggacag ccttttgctc taatcaatta cgcctcggcg taattgcgtc
     3421 cagattttgc gaatcgaaca gcgcagggtt ctattcgcaa ttcactccct tcaaaatctg
     3481 tgacatccgc cggaggcatt aacttgcttc agcaagttag taggttgtca gtttcaacaa
     3541 ctcctgaagg cggtagatgc cgcgttggaa gaatgtgaag tcatcctggt actcctcgaa
     3601 gtcgagtgtg taaagggcgt catcattatt ccaaagccgg tcgaaatagg catcgatctc
     3661 cttggacaac tcgctgtcat tcggcgcgat gacccttaag ttcgcttcga gattgtaatt
     3721 gttcaatgta cggtctgtaa gattcgcgga gccgttcgta atatacgtct cctgtttcgt
     3781 ctggatgacg acgagcttcg tatggtattg gccgactacc gtattatacc agcggacatc
     3841 gagctttccg tccgtctcct ccatcatctt ctggagtacg ggacggttcg gaaggcccga
     3901 cttctcattg ccgaacgagt tttcattcgg atccaaaatc atctggacgt gaactccccg
     3961 attcgaagca tcaacaatcg cctgcacgat atcgggcatc gcaatgaaaa acatgcccat
     4021 ccggatcgta tccccttcct gcgccgccgc aatatcgccg agaagcgcat catgaatctt
     4081 cttttccgtc aaatattgga ctgcataatc gccgtcatgc tccttttcaa catccgcacg
     4141 cggcagcgag ccgccattcg tgtagcggac gaccgcctct tccgcctcca aaatatcatt
     4201 caatacggtc cctttcactt tcaaggcgac attgccatgg aggccgctcg cgtcatgcgg
     4261 attgcccgac gtcacgatgg ccgacttgtc cgtcacgacc gtcttccgat ggttcgcctt
     4321 cacattcagg aggatcaagt acgatcgcag cgtcattttc ggcgctttgc tcgacatcgc
     4381 attggcaatc cagccttctt tttcgaaatt gacccagcgg aaaatggtcc ggtagagtcc
     4441 cgaatagatc ggcgtcgagt cccgcagctt atcgagatca gaatagacga cttcgatgcc
     4501 ggcatcctcc agccggctga accatttcga ttcataagag ccatatcctg tgttgagggg
     4561 atcagtaata taaataattt tcatttccgg attctctttt ttcttttgaa tcaatttctc
     4621 cgtcaacgtc tccgcgattt tcggaaagtc gatgttttca tccgtgtaat ggtccatgag
     4681 gaagaaatca aggacgatga actcctccgc ctcgtcgatc atgttgtaga tttcgtcaaa
     4741 gatgttgagc tcatgcacca tcccatcgcc ttttttgttt tgggcgtacg tcagatcggt
     4801 cagcatttca acgtcatcgg tccaatggat gtcgccttcg tagttgaccc cttccggaag
     4861 cggtttatat gtatgccagg tgatgacccc gacgtatagg aacaagaata gcaggatggc
     4921 catgaggacg atccacttcg gctgcaccca tttcttcatt tttttcattc aatcaacccc
     4981 gcattctact tgtctattca aattccccta acttgctgga tgcaaactat gttgttggaa
     5041 aaaataaaat gcccctattg atagaggcat tccatacaga tattcaatta agcagtgaca
     5101 tattctctca ctgtcgtgtc gtccatcatt agttctttca agtacttttt cgcgcggaac
     5161 aggcgtgatt tcactgtgcc gattgaaact ttcatcaaat ccgcgatttc gatcaaggaa
     5221 tattggtaga cgtagaagta ttcgatcgtt gtccggtaaa tgccgtccaa ttggccgatt
     5281 ttctgctgca ccatgagctg gatatcgttt tgctcgatca tttcgccagg tgtcggattg
     5341 tcgctgggaa ccaaatccaa gatcggaacg tccaatgttt caggttggtg cataacgtgc
     5401 ttgcttcgtc tgacatcctt ccggtaacga tcgcggaatg tgttcatgca gattgtcgtc
     5461 agccaagctt tcatgcggtc cacactgtcc atctttccgc tgtagcggac aaccttgacc
     5521 catacgtctt gcataaggtc ctcggcttcc tctttattgc gcgtcagttt taaacataaa
     5581 tggtatatgt atcggccata ttcgtcatat agctctgtca agtcgatttt catgcgataa
     5641 ctcctccgtt ttgtaactgt tatcctcatt atcaatgaat ctcccattac aaactatcgc
     5701 tttgtctttc actaaactta caaatgtgta agcaaacaaa agcggagggc gcttgctatt
     5761 ctgctcgtaa cgctgcgctt ttactcgcaa aagctgttct tcgttacagc tttagctgcg
     5821 acaggcataa ggcgaagatg cgaagtggcg ctttttgcca cacagcagct acgacttatg
     5881 acccgagcag ctagcgcccg tagctggatg tctcgaaagt tttgctcgag ttcggcagtg
     5941 ttatggcgca ttcttgatga gttatggcgc aattgggcgg aattatgctc gagtttggac
     6001 cagttatggc gcaatctcgg caagttatgc tcatctcgtg acctatagcc tagcgaatcc
     6061 acatgaaaaa acaccccgca atcattctgc ggggcgatca ttttcctgat catgcctttt
     6121 cgtgatcttc atcttgatga tgcggctgcg atccatttcc tcgatttcga agtacaggtt
     6181 ttcgtatgta aaacgttccc cttcatcggg tacatgacca agctcttgca tgacgaatcc
     6241 accgatcgtt tcatgctccg tctccaattc gacgtccagc aggtcgatgg catcgacaat
     6301 ttcaaggcgt ccatgacaga tgagcagttc ctcgttcttt tcatagacga gcacttcttc
     6361 atcgaagtcg gtttcatcct cgatatcctg cccgatcatc tcctcgatga tatcctcatg
     6421 cgtgacgatg ccgagcgttc cgccatattc atcaagcacg atggccatat gttttttatg
     6481 cgcaagcatc agcttgaaca ctttttcgac gcttgctgtc tgtacgacga ataaaggctc
     6541 ttcgtccatc aagtcggcaa gcttcttttc aggattcatg gaccattcga tcagcgtctt
     6601 cgaataaaag attccgacga tccggtcgat gctttcctcg taaatcggat agcgtgtata
     6661 gaagtattcc aaaatcgtat cgcgcacttc ttcgtatgtc gattcgatcg gcagcccgat
     6721 cacatcggta cggtgcgttt ccatcacatc ggagacgtct ttttcaggaa aatcgagcac
     6781 acctttcaac cggagcgatt cctcctgttc gaatgtccct tccgtcgaag cgatgtccac
     6841 catggaacgg agctcttcct tcgtcaacgt cgcttccgtc acggcacctt tcgagatgat
     6901 gcgtatgaag atgttcgtga ataacgagag gagaatcgtc agcggcgtca gcagtttgac
     6961 gaataccgca attgtaggcg cgacgacata agcgatccga tcggcaaatg tcgctgcaat
     7021 cgtcttcggc aatacctccc cgaatacgat aagcacgacc gtcaaaatcc ctgtcatgac
     7081 tcccactttc aagccacgat cgatcgcgat cattgtgacg attgtcggca tcattatatt
     7141 tgctacgtta tttccaatga gaatggccgt gatcattttg tccggctttg atatcaagtc
     7201 tctgagcttg atcgatttcg gatcgccttg ttcggcacga agctggactt tcatgcggct
     7261 gaccgcggtc agtgccgttt cactccccga aaggaagaac gacataagta aaaagaaccc
     7321 aagtgctata aacaaaagtg attcctcccg gagtttctgt acttcaacag ttcgttcatt
     7381 tagcaacttt tacatatcat aacacaatga tagcaaattc gtccttttgg ataacttgat
     7441 tccctagcga catgctatac tatttttatt cacgaaataa ttttgaaatg aggggttcat
     7501 tttgagtaat ttggaaaagc ttgacgctta tttcacatcc aaacgggaag agcatttgga
     7561 gcaattgaaa gatttccttc gtatcccgag cattagtgcg ttgtctgagc ataagtcgga
     7621 catgcagaaa gcggctgaat ggttgaagga ttcattccgc aaggcgggat tggagaacat
     7681 gtcgatcgat gagacggaag gacatccggt cgtctacgga gactggctcc acgcggaagg
     7741 caaaccgaca atcctctttg atggacacta cgacgtccaa cctgtcgacc ctttgaacct
     7801 ttgggaaagc ggtccgtttg atccggaagt gcgcgacaat aaattgtatg cgcgcggggc
     7861 aagtgatgac aaaggccaag tttttatgca tattaaagcc gtcgaagctc ttatgaatga
     7921 agacggaagc ttgccggtga acgtcaaatt cattattgaa ggcgaagagg aaatcggc
//
LOCUS       DASULI010000224         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1227993,
            whole genome shotgun sequence.
ACCESSION   DASULI010000224 DASULI010000000
VERSION     DASULI010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227993"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3415)
                     /gene="mfd"
                     /locus_tag="VFX34_04895"
     CDS             complement(<1..3415)
                     /gene="mfd"
                     /locus_tag="VFX34_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX5564277.1"
                     /translation="MEALTDLFLREKEIGTIVEELENGKDRQLIAGLSGGAKPIFIHA
                     LKKMLHKPMLIVSPNLLQAQRIYEDLAKLVGEENVHLYPAEELIAADFSFSSFDLRAQ
                     RIETLDHMGRIGKGIYITPIAGMRKFLPQKERFFSSCLETSVGDELAIEDVLTKLVQM
                     GYSRQQMVTAPGEFALRGGILDVYPLYMENPVRIELFDTDVDSIRTFSADDQRSISKL
                     ETVRILPATEFVLQKSEYYEIAEQLEKSLGNSLKKMTNSEAKENMTLHIQGDIELMRT
                     GEPPEQIGKYVSLLPEAPSFLGDYFPADGIVVFDEIGRVLEVIQSLEKEEHEWFVSLL
                     EEGKIVHDAKLSFTFSEFHSMLEQAKVYLSLFIRSIPGIQVKRTLSISCKPMQHFHGQ
                     MNLLKNEMERWQHSGFTIFIVADGKERMMKVQSVLADYGMETELSGKTSKSGQIVLVD
                     GDLSAGFELPLQRIAVMTDAELFKQKTKKKARPQKMTNAERIKSYSEIKPGDYIVHVH
                     HGIGKYVGLETLEVNGTHKDYLHIRYRADDKLFVPADQIDLIQKYVPSGEKEPKLHKL
                     GGTEWKKTKTKVSAAVKDIADDLIKLYAKREAEKGFAFSPDDDMQRAFENAFPYDETE
                     DQLKSIEEVKIDMERDRPMDRLLCGDVGYGKTEVAIRAAFKAVTDGKQVAFLVPTTIL
                     AQQHYETMKERFAGFPVEVALMNRFRTKKEQDETLKGLKAGTVDVVVGTHRLLSKDII
                     YKDLGLLIVDEEQRFGVTHKEKIKQLRTNVDVLTLTATPIPRTLHMSMIGVRDLSVIE
                     TPPANRFPVQTYVMEHNYALIREAIERELSRGGQVFYLYNRVEDMTRKVEEIKDLVPD
                     ARVGYAHGKMTERELESVILSFLEGEYDVLVTTTIIETGIDIPNVNTLIVHDADRMGL
                     SQLYQLRGRVGRSNRVAYAYFLYQRDKVLTDVAEKRLQAIKEFTELGSGFKIAMRDLS
                     IRGAGNLLGSQQHGFIDSVGFDLYSQMLQEAIDERRSGTNKEEEPDLEISLPVDAYIP
                     DEYVKDGYQKIQMYKRVKAIESEEDYNELIDEMTDRFGDMPLETDLLLRIARIKVWGR
                     QAGVESIKKHRNLIEVRLSEEGTAKTDGGKLVSESMKFGRAVGFSMDGKQ"
     gene            complement(3482..3712)
                     /locus_tag="VFX34_04900"
     CDS             complement(3482..3712)
                     /locus_tag="VFX34_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-F factor Fin"
                     /protein_id="HEX5564278.1"
                     /translation="MAIRYKCRHCATEVGSLPFGSAKETVRMLHQLNEGEEENFVTTC
                     EKGHLTVQCICEQCEKSLREFPDYYSLGKWLQ"
     gene            complement(3768..>4049)
                     /locus_tag="VFX34_04905"
     CDS             complement(3768..>4049)
                     /locus_tag="VFX34_04905"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5564279.1"
                     /translation="LPPGQIRLRQKGSAGGHNGMKSIIQHIGTQEFNRIRIGIGRPPA
                     GMKVADYVLSSFLKEESAVIEDMIKKSADACIDWIGKPFLAVMNDFNGS"
BASE COUNT          996 a         1053 c          796 g         1204 t
ORIGIN      
        1 gctgtttacc gtccattgaa aatccgaccg cgcgaccgaa tttcattgat tccgacacaa
       61 gttttcctcc atcggttttt gccgttcctt cttcagataa tcgaacttcg ataaggttcc
      121 gatgcttttt aatcgactcg acgcctgctt gacgacccca caccttaatt cttgcaatcc
      181 gcagcaacaa gtccgtttcc aatggcatgt caccgaatcg gtctgtcatt tcatcgatca
      241 gttcgttgta atcctcttca ctttcaatcg ctttaacccg tttgtacatc tggatttttt
      301 ggtagccgtc cttgacgtac tcatccggga tatatgcatc aaccggcaat gagatttcca
      361 gatcaggttc ttcttcttta ttcgttccgc ttctacgttc gtcaattgct tcctggagca
      421 tttgagaata tagatcgaac ccgacagagt caataaatcc atgttgctgg gaacccaaca
      481 agtttcctgc tccccggatg gataaatccc tcatggcaat cttaaacccg gacccaagct
      541 ctgtaaattc cttgattgct tgcaaacgct tctctgctac gtctgttagc accttgtcgc
      601 gctgatagag gaaataggca tatgccacac gattcgagcg gccgactcgt ccacgaagct
      661 ggtagagctg ggacaagccc atccgatctg catcatggac gatcaatgta ttaacatttg
      721 gtatatcaat tcctgtttca atgattgtcg ttgttacgag gacatcatac tcaccttcca
      781 aaaagctaag gattactgat tcaagctctc gttctgtcat ttttccatgt gcgtagccga
      841 ctcttgcatc cggcacaagg tctttgattt cctcaacctt ccgcgtcatg tcctccaccc
      901 ggttgtagag atagaagact tggccaccac ggcttaattc ccgttcaatc gcttcccgaa
      961 tcaaggcgta attatgttcc atcacatacg tttgaaccgg gaaccggttt gccggcggcg
     1021 tttcaattac cgataagtcc cgtacgccaa tcatggacat atgaagggtc cgcggaatcg
     1081 gcgttgcagt caacgtcaga acatcaacgt ttgtccgcaa ttgtttaatc ttctccttgt
     1141 gggttacacc gaaacgctgc tcctcgtcga caattaaaag acctaaatct ttatagataa
     1201 tgtctttgga taaaagacgg tgcgttccta caacaacatc aaccgttcca gcctttaatc
     1261 cttttaacgt ttcgtcctgc tcttttttcg tacggaaacg gttcatgagt gccacttcta
     1321 ccggaaaccc tgcaaaacgt tccttcattg tttcataatg ctgctgggcg aggatggttg
     1381 tcggaacaag aaacgcgact tgcttcccat ccgttaccgc tttgaaagcg gcgcgaatgg
     1441 caacttccgt tttaccgtag ccgacatcac cgcaaagaag acggtccatc ggtcgatccc
     1501 gctccatatc tatcttcact tcttcaatcg acttcaactg atcttccgtt tcatcatatg
     1561 gaaacgcatt ttcaaatgcc cgttgcatat catcgtcggg tgagaaggca aagccctttt
     1621 ccgcttcccg tttcgcatac agctttatta agtcgtccgc aatgtcctta actgccgctg
     1681 ataccttcgt ttttgtcttt ttccattctg ttccgcctag cttgtgcaac ttcggctctt
     1741 tttcacctga cggaacatat ttctgaatca aatcaatttg atcagccgga acaaacagct
     1801 tatcgtctgc gcgatagcgg atgtgaagat aatccttatg cgttccgttt acttcaagcg
     1861 tttcaaggcc aacgtatttc ccgattccat gatgaacgtg gacaatgtag tcgccaggtt
     1921 tgatttcaga atagctttta atccgctcgg catttgtcat cttttgcggt cgagcttttt
     1981 tcttcgtttt ttgtttaaac agttcggcat cggtcatgac tgctatccgt tggagaggca
     2041 gctcaaatcc agcggacaaa tcaccgtcca ccaatacaat ttgtccgctt tttgaggttt
     2101 ttccggataa ctccgtttcc atcccataat ccgctaagac cgattggact ttcatcatac
     2161 gctctttacc atctgcaaca atgaatatag taaaaccgct atgctgccaa cgttccattt
     2221 cattttttaa taggttcatt tgaccatgga aatgctgcat cggcttacat gagattgaga
     2281 gtgtcctttt tacttgaata cctggaatgg agcgaatgaa taatgacaaa tacacctttg
     2341 cctgctccag catggaatgg aattcagaaa acgtaaatga aagtttcgca tcatgtacaa
     2401 tcttaccttc ctcaagaaga gacacaaacc actcatgctc ttctttttcg agtgattgaa
     2461 tgacctccag gacgcgaccg atttcatcga aaactacaat accgtctgcc gggaaataat
     2521 caccgaggaa agaaggtgct tctggtaaca atgaaacata ctttccgatt tgctccggtg
     2581 gttctccggt tcgcatcagc tcgatgtctc cctgaatgtg gagcgtcata ttttccttcg
     2641 cttctgaatt cgtcattttc ttcaaactgt ttccgagcga cttctcaagt tgttctgcaa
     2701 tctcgtagta ctctgatttt tgaagaacaa attctgtcgc cggtaaaatc cgtacagtct
     2761 ccagcttcga aatggagcgt tggtcatccg ccgagaaagt ccggatggag tcaacatccg
     2821 tatcaaacag ctctatacgt actgggtttt ccatatataa agggtacaca tctaaaatgc
     2881 cgccacgcag cgcaaactcg ccaggtgcag tgaccatttg ttgacgcgaa taccccatct
     2941 gcactaattt ggtcagcacg tcctcaatgg ccagttcatc tccgaccgaa gtttccagac
     3001 agctcgaaaa aaaccgctcc ttctgcggca agaatttccg cattccagcg attggcgtaa
     3061 tatagattcc ttttccgata cgccccatat gatcaagtgt ttcaattcgt tgcgcccgta
     3121 ggtcaaagct cgaaaaggag aagtcagctg caatcaattc ctctgcagga tacagatgta
     3181 cattctcttc ccctaccaac tttgctagat cctcgtatat acgctgtgct tgcagtagat
     3241 tcggtgaaac gatcaacatg ggtttgtgga gcatcttctt taatgcatga ataaatatcg
     3301 gttttgcgcc gccggaaagt cctgcaatca gctgccgatc cttcccattc tccaattctt
     3361 ccactattgt cccaatctct ttttcccgta gaaataaatc ggtcaacgcc tccaaagcgg
     3421 cacttccttt cttatgttaa agcagaaaaa gctctggacg ccttgcagcg ccaaagcgat
     3481 tctattgcag ccattttccg agtgaatagt aatccgggaa ttcccgcaat gatttttcac
     3541 attgttcgca aatgcattgt acggtcaaat ggcctttttc acacgttgtc acaaagttct
     3601 cttcttcccc ttcgttcagt tgatgcaaca tccgcaccgt ttctttcgct gaaccaaatg
     3661 gaagactgcc tacctccgta gcacaatgcc tgcatttata acggattgcc atcgattgat
     3721 ccgccccttt tttacaaagt ataggcggaa cgaatgtctg ttattcctta cgagccattg
     3781 aaatcattca tgacggcgag gaatggttta ccaatccaat cgatgcacgc atcggcactc
     3841 ttcttaatca tatcttctat aacggcagat tcctctttta gaaatgaaga caatacataa
     3901 tctgctacct tcattccagc aggagggcgg ccgattccaa tccgaatccg attaaattcc
     3961 tgtgtgccga tatgttgaat aatcgatttc attccattat gcccgccggc actgcctttt
     4021 tggcggaggc gaatttggcc gggtggcaa
//
LOCUS       DASULI010000225         4551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_274354, whole
            genome shotgun sequence.
ACCESSION   DASULI010000225 DASULI010000000
VERSION     DASULI010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274354"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..369)
                     /locus_tag="VFX34_04910"
     tRNA            complement(295..369)
                     /locus_tag="VFX34_04910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(335..337),aa:Gln,seq:ttg)
     gene            complement(395..479)
                     /locus_tag="VFX34_04915"
     tRNA            complement(395..479)
                     /locus_tag="VFX34_04915"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(443..445),aa:Tyr,seq:gta)
     gene            complement(564..638)
                     /locus_tag="VFX34_04920"
     tRNA            complement(564..638)
                     /locus_tag="VFX34_04920"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Glu,seq:ttc)
     gene            complement(794..869)
                     /locus_tag="VFX34_04925"
     tRNA            complement(794..869)
                     /locus_tag="VFX34_04925"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(834..836),aa:Asn,seq:gtt)
     gene            complement(877..951)
                     /locus_tag="VFX34_04930"
     tRNA            complement(877..951)
                     /locus_tag="VFX34_04930"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(917..919),aa:Asn,seq:gtt)
     gene            1123..1788
                     /locus_tag="VFX34_04935"
     CDS             1123..1788
                     /locus_tag="VFX34_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564280.1"
                     /translation="MRAKLLLLIFVCCAAALFFPSMPGSGSLKSVFRLTDEPNEVIVK
                     GMSGSALTINISFGDKEVEQLVRELQAPYPLLLLDMDWAERFPEIVQLIKKRNIPTGL
                     LGHAGMDYEQNTPLLIEQLDKYENLFGSKPLWFRTSDEQFPYSLRGALWDSEVNALGS
                     SVRWHTGEPPPVIEGEIISLPHHREERITLTDFKKLTETRQNKSLEDVLFSMSIKTKK
                     MPE"
     gene            complement(1832..2509)
                     /locus_tag="VFX34_04940"
     CDS             complement(1832..2509)
                     /locus_tag="VFX34_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KinB-signaling pathway activation protein"
                     /protein_id="HEX5564281.1"
                     /translation="MTIRNWVKFFFTALIIGGVVTGILGLIVRWEFFSQYLSTGEYME
                     FLGALLWMIFLGFTMSVVAQMGFFAYLTVHQFGVNLFKSLTLWNWVQLLIIAVVIFDL
                     VFFRFKLTAGETGRTILYLTLLFSLLAVSIVTAYLKAKWTKKHTLISALFFMIVITTL
                     EWLPALMVRSGNIDSWVTILLFPLLAVNAYQLLMLPKYNRQSDEDKARLDARKQARNS
                     AKTAKAK"
     gene            2652..3275
                     /gene="gerD"
                     /locus_tag="VFX34_04945"
     CDS             2652..3275
                     /gene="gerD"
                     /locus_tag="VFX34_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination lipoprotein GerD"
                     /protein_id="HEX5564282.1"
                     /translation="MMKKWVHLLVLAILLSGCSMQQQGPNYDEMKKMMTDALQTDDGK
                     KAVRKMLSDPEFRELMVLEQSEVKKSIEDTLLSKKGEDFWKHAFEDPKFSEAIAKSMK
                     EQQQDIMTKLINDSSFQKEMVEFFGQPDMQKQMETILQSANMKKQMEKSIEETINSPL
                     LKAKWQELILKAGEVTPTGKGGGGESGGGGGGGQSGGGNEKSGGGGK"
     gene            complement(3292..4350)
                     /locus_tag="VFX34_04950"
     CDS             complement(3292..4350)
                     /locus_tag="VFX34_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX5564283.1"
                     /translation="MINEQNVRDVVGELKDPFLHKTLAETDGIVSVSVKPEKKHVSVK
                     LAIAKVNTAEQMNLQMKVVEVIKAAGADSVGIRFEELPAETLEKFRGTATEAEAQDLL
                     SPLNDIEFISIASGKGGVGKSTVSVNLAVALARLGKKVGLIDADIYGFSVPDMMGITE
                     LPVVKENRIIPVDRFGVKVISMGFFVEDNAPVVWRGPMLGKVLDQFFRDVDWGPLDYL
                     ILDLPPGTGDVALDVHQMIPSSKEIVVTTPHPTAAFVAARAGAMALQTDHALLGVIEN
                     MAWFESKLTNEKEYVFGQGGGTRLAEELRTELLGQIPLGQPDWNETDFAPSVYAEHHP
                     IGETYMKIAQAVIDKTQK"
     gene            complement(4429..4539)
                     /locus_tag="VFX34_04955"
     CDS             complement(4429..4539)
                     /locus_tag="VFX34_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564284.1"
                     /translation="MSNPEERALLTDPAYHGKVADAIVDGIVQYRMSDKQ"
BASE COUNT         1272 a         1083 c         1006 g         1190 t
ORIGIN      
        1 ctagcttctc tactatacct ttctatgata cccgtaaatc tatataagat gggttagctg
       61 aatgaacatt tttcgaaagt gttgggggat tttaggcaat aaaaaaaatc ctatcttttg
      121 atggatttga gtgggtttgt atgtggtttg tggggttgtt atttatgtta atttctaatc
      181 aataaaaccg ttttgctcct aattccttcg gaaattttaa taaaaccgat ttgctcgcaa
      241 ttaccttgcg gcattgctcg caaaactttt ttatcgcgaa gcgctaaaaa agtttggctg
      301 gggtagctgg attcgaacca acgaatgacg gagtcaaagt ccgttgcctt accgcttggc
      361 tataccccaa cagcagcgat taagtatgta taaatggtgg tgggggacgg attcgaaccg
      421 ccgaacccgg agggagcgga tttacagtcc gccgcgttta gccacttcgc taccccaccg
      481 tattaaaaat gcgatctgct tgcttctccc aatttttatc gggaatttat gataagtata
      541 tgtcttgaaa aaaggaacaa acatggtgac ccctacggga ttcgaacccg tgttaccgcc
      601 gtgaaagggc ggtgtcttaa ccgcttgacc aaggggccat ctactaattt aataatgttt
      661 catgaatcgc acaaatgtta tagtaacaaa tttagattca ttatgcaagc ctatttttca
      721 tttcttccgc tgcagcagca ggcggacata aaaaaagcgt cttccctatg aaacagaaat
      781 cgcaagtttc atctggagct tccaaccgga ttcgaaccgg tgacatcttc cttaacatgg
      841 aagcactcta cctactgagc tatggaagca taataatggc tccgcaggta ggactcgaac
      901 ctacgaccga tcggttaaca gccgattgct ctaccactga gctactgcgg aatcgtaatt
      961 aattttccca acgacattat tcattttatc ggaaagccgg gtgaaaatca accttaattt
     1021 cattgccccg taacgacttt cttatattag catgttccaa tcatattggt caagccatta
     1081 tcataaagta ccctatagat tccgtttcaa aggggatgtg acttgagagc aaaactatta
     1141 cttctcattt tcgtttgctg cgctgccgca ctattttttc caagcatgcc cggatcgggt
     1201 tcattgaagt ctgttttccg cttgacggac gagccgaatg aagtcatcgt caaaggaatg
     1261 tcgggatccg ctttgacaat taatatttca ttcggggaca aggaagtgga gcagctggtc
     1321 cgtgaattac aggcacccta tccactgctt cttctggata tggattgggc cgaacgcttt
     1381 ccggaaatcg tacaattgat caagaaacgc aacatcccga caggcttatt gggacatgcc
     1441 gggatggact atgagcagaa cacacctctt ctgattgagc agctagataa atacgagaat
     1501 cttttcggct ccaaaccatt gtggttcaga acgtccgatg agcagttccc ttattctttg
     1561 cgaggggcac tttgggattc cgaggtgaac gcccttggct catctgtccg ctggcacacg
     1621 ggagagccgc cgccggtcat tgaaggagaa atcatttcat tgccacatca tcgtgaggag
     1681 cggatcacgt taacagattt caaaaaactg accgaaacgc ggcaaaataa atcattggaa
     1741 gacgtcttat tcagcatgtc gatcaagacg aaaaaaatgc cggaataaac aaaagcgcag
     1801 ttcccatcca tttgagggac tgcgcttttc atcatttcgc ctttgctgtt tttgccgaat
     1861 tacgggcctg tttacgtgca tctagccttg ccttgtcctc atcggattgg cggttgtatt
     1921 ttggcagcat tagaagctga taggcattta ccgcgagaag cgggaataat aaaatcgtca
     1981 cccatgaatc gatattgcct gaacgcacca tcaatgccgg cagccattca agcgtcgtga
     2041 tgacaatcat gaaaaacaat gcggaaatta gcgtgtgctt tttcgtccat ttcgccttca
     2101 aatacgctgt gacgatggac actgccagta atgaaaacag caacgtcaaa taaaggattg
     2161 tacgccctgt ttctcctgct gtcagcttga atctgaaaaa taccaagtca aagataacaa
     2221 ccgcgatgat cagcagttgc acccaattcc ataacgtcaa tgacttgaat aagtttacgc
     2281 cgaactggtg caccgtcaag taggcgaaga agcccatttg cgctacgaca ctcatcgtga
     2341 acccaagaaa gatcatccat aagagagcac cgaggaactc catatattct ccggtagata
     2401 gatattgtga aaagaattcc caacggacga tcaaccctag gatccccgtg acaactcccc
     2461 cgattatcaa agcagtaaaa aagaatttaa cccaatttcg aatggtcacg atgctgttcc
     2521 cccgttttta attactctta ccagtgtatc aacgttttgc cgaaaaatct atttcattgg
     2581 atgcacaatg catattcctc ttcatattgt gaacaataat tgaaaacgca ttcggaaggg
     2641 aaattgatct catgatgaaa aaatgggttc atttacttgt acttgccata ctcttatcgg
     2701 gctgcagcat gcaacagcaa ggaccgaatt atgatgaaat gaagaagatg atgacggatg
     2761 ccctgcagac ggatgacggg aaaaaggctg tccgtaaaat gctttccgat cccgagttca
     2821 gggaactgat ggtgttggag cagtcggaag tgaaaaaatc catcgaggat acgttattgt
     2881 cgaagaaagg cgaggatttt tggaagcatg cattcgaaga tccaaaattc agcgaagcaa
     2941 tcgcaaaaag catgaaagag cagcaacagg atatcatgac caagttaatt aacgactctt
     3001 cattccaaaa ggaaatggtg gaattcttcg gccagccaga tatgcaaaaa cagatggaaa
     3061 ccatattgca atcggcgaat atgaaaaaac aaatggagaa gtccattgaa gagacgatta
     3121 acagtccatt gctcaaagcg aagtggcagg aactgatcct caaagcaggt gaagtgacac
     3181 cgaccggaaa aggcggtggc ggtgaatccg gcggcggtgg tggcggcgga caatccggag
     3241 gtggaaacga aaaaagcggc ggcggaggca aatgacctcc accacccgct tttatttttg
     3301 tgttttatcg ataactgcct gtgcaatttt catgtacgtt tcgccaattg gatgatgctc
     3361 cgcatagacg gatggggcaa aatcggtttc attccaatcc ggttgtccga gtggaatctg
     3421 gccaagaagc tcggtacgta attcttctgc caaccgagtg cctccgcctt gtccgaatac
     3481 gtattccttt tcgtttgtca gcttcgactc aaaccaagcc atgttttcaa taacgcctaa
     3541 cagagcatga tccgtttgga gagccatcgc ccccgcacgc gccgccacaa atgcggcagt
     3601 cggatgagga gtcgtcacga caatctcctt cgaggaagga atcatttggt ggacatcaag
     3661 cgcaacatcc ccagttcctg gcggcaagtc caggataaga tagtccagcg gtccccaatc
     3721 gacatcgcgg aagaactggt caagcacttt tccaagcatc ggaccgcgcc atacgaccgg
     3781 agcattgtcc tctacaaaga accccatgga aatcactttt acaccaaagc gatctaccgg
     3841 aatgatccgg ttttccttga cgaccggcag ctcggtgatc cccatcatat ccggcacact
     3901 gaacccgtaa atatccgcgt cgatcaatcc tacctttttc ccaaggcgcg cgagtgctac
     3961 cgccaagttc acggaaacgg tcgacttccc gactccgcct ttcccggatg caattgaaat
     4021 gaattcaata tcattcagcg gagacaataa atcctgtgct tccgcctccg ttgctgtacc
     4081 gcggaatttt tctagcgttt cggctggaag ctcttcaaat cggataccga ctgaatccgc
     4141 acctgccgcc tttatcactt caactacttt catctgcaaa ttcatttgtt ccgccgtgtt
     4201 gactttagcg atggccaatt ttacactgac atgcttcttc tcgggcttga ccgatacgct
     4261 tacaattcca tcggtttccg ccaaagtttt atgtaaaaac ggatccttca actcgcctac
     4321 aacatcacgg acattttgtt cgtttatcat tttggccact ccttagaggt ttattcattc
     4381 tcctctaagt ataccatatt gattagtctg caaagttagg aatcagcttc actgtttatc
     4441 ggacatacga tattgaacga tgccatccac gatggcatcc gccactttcc catgataggc
     4501 agggtccgtc aacaatgccc gctcctcagg attggaaatg aaacccgttt c
//
LOCUS       DASULI010000226         4968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_831, whole
            genome shotgun sequence.
ACCESSION   DASULI010000226 DASULI010000000
VERSION     DASULI010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /gene="typA"
                     /locus_tag="VFX34_04960"
     CDS             complement(<1..1500)
                     /gene="typA"
                     /locus_tag="VFX34_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5564285.1"
                     /translation="MTNNRNDLRNIAIIAHVDHGKTTLVDQLLKQSGIFRSNEHVEER
                     AMDSNDLERERGITILAKNTAVEYKETKINILDTPGHADFGGEVERILRMVDGVVLVV
                     DSFEGCMPQTRFVLKKALESNIKPIVVVNKIDRDFARPEEVVDEVLELFIELDANDEQ
                     LEFPVVYASGFNGTASLSPDPADQDDTLQVLFDAIVEHIPAPIDNREEPLQFQVSLLD
                     YNDYVGRIGIGRVFRGTMKVGQQVTVLKRDGSTKNFRVTKMYGFLGLKRIEIQEAYAG
                     DLIAISGMEDLDVGETVCPVDHLEALPELHIDEPTLQMTFLVNNSPFAGKEGKWVTAR
                     KVEERLEQQLQTDVSLRVDPTDSPDAWVVSGRGELHLSILIENMRREGFELQVSKPEV
                     IIRTIDGVRSEPVERVQIDVPEEYTGSIIESIGERKGEMQDMINNGNGQVRLIFLVPA
                     RGLIGYSTEFLSLTRGYGIINHTFDSYKPVAQGRVGGRRHGVLVSMETGT"
     gene            1597..1782
                     /locus_tag="VFX34_04965"
     CDS             1597..1782
                     /locus_tag="VFX34_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017554426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HEX5564286.1"
                     /translation="MKDIKWVFVFYSLAAVAAMCGIGIAVAMRSIPVAIIAILALIFV
                     MGNGFKTKKKMREQGIL"
     gene            complement(1947..2738)
                     /locus_tag="VFX34_04970"
     CDS             complement(1947..2738)
                     /locus_tag="VFX34_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5564287.1"
                     /translation="MNWGAIDRYAKSLIKEAGHKIRISFFSTIDVDSKAEANDLVTNI
                     DQEIERFFISRIKRDFPGHRIFGEEGFGDEIESLDGIIWMLDPIDGTMNFVHQKRNFA
                     ISLGIYADGIGMLGYIYDVMNDDFYHAVKDEGAYFNDERLPVLEETPLAEAIIGVNAS
                     WVAPNRRIENGPIAEMVRHCRGTRSYGSAAIELAYVSSGRIDAYLSLRLSPWDIAGGI
                     VIAQEVGAVTTNLKGEQPHLLGQDTFIVARPGLHDEILRNYIKLK"
     gene            complement(2877..3074)
                     /locus_tag="VFX34_04975"
     CDS             complement(2877..3074)
                     /locus_tag="VFX34_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564288.1"
                     /translation="MKQVVHIIRKAEIEKEYVKLLHLELDYELATLYDAVQENDSKQV
                     EKSKTRLTEIHGELETFNAFA"
     gene            3234..3860
                     /locus_tag="VFX34_04980"
     CDS             3234..3860
                     /locus_tag="VFX34_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1054 domain-containing protein"
                     /protein_id="HEX5564289.1"
                     /translation="MTQGFWTSDDFNVFSIEGLEHRMNGLQTKIQPKFQVIGEQISMY
                     LSSRGTGEFFPHVAKHARRTVNPPNDSWVAFAPAKRGYKALPHFQIGLWGSHLFIILA
                     VIYENPDKKGIAERLSQKVNVLTSLPEDFVVSGDHMKPEAFRINDIGTEGVERLLERL
                     SSVKKAEFIIGKHLVRDDATTLSKKEFFNFAEETIDRLFPIYNIIIGR"
     gene            complement(3899..4171)
                     /locus_tag="VFX34_04985"
     CDS             complement(3899..4171)
                     /locus_tag="VFX34_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEX5564290.1"
                     /translation="MDYNYPLSHHWTTEEIIDVVALFQAVEKAYEAQIERSTFMAAYK
                     KFKKVVPSIAEEKTIFKEFEEASGLNSYRIVKKAKEGSDGDYISFK"
     gene            complement(4190..>4968)
                     /locus_tag="VFX34_04990"
     CDS             complement(4190..>4968)
                     /locus_tag="VFX34_04990"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX5564291.1"
                     /translation="VRELTAKPFGVNFAIGQHGRPYEHLLDVALEEKVKVLSVTGGNP
                     APFFDYVGDADVKKLVLVAAKRQAVKAESLGADAVMVVGQEGGGHLGRDDIGTMVLVP
                     QVVDAVKIPVIASGGIGDGRGWMAAHALGAEGIEMGTRFIATKECVHASEEYKQALLQ
                     TEETGTVVIKRSLGAPARALSGSWTEKILRMEAENADYDKLKDYISGVANRRFIHNGD
                     EQSGFGWAGQVAGMIEDVPSVAELIVRMVKQAEDIRRKWS"
BASE COUNT         1245 a         1244 c         1001 g         1478 t
ORIGIN      
        1 tgttccagtc tccatagata cgagcacgcc atgtctacgg ccgccgactc tgccttgcgc
       61 tacaggttta taggaatcaa acgtatggtt gatgatccca tatcctcgcg taagcgatag
      121 gaactctgta gaataaccaa tcagaccacg agcaggaact aggaagatca aacggacttg
      181 tccattgccg ttattgatca tatcctgcat ttcgcctttt cgctcaccga tcgattcaat
      241 gatggatccg gtatattctt ccggtacatc aatctgtaca cgttcgacag gctcagaacg
      301 aactccatcg attgttctga taataacttc aggttttgac acttgaagtt cgaatccttc
      361 gcgtcgcata ttttcaatca agatggaaag gtgcagctct ccacgtcccg atacaaccca
      421 tgcatccggt gaatctgtcg gatcaacacg taaagaaaca tctgtctgca attgctgttc
      481 aagtcgttct tcgacctttc tcgcagtcac ccatttgcct tctttccctg caaacggact
      541 attattgacg aggaaagtca tttggagtgt cggctcatca atatgaagct ccggcaacgc
      601 ctccagatga tcgaccggac aaaccgtttc acctacgtca agatcttcca tacctgaaat
      661 agcgatcaaa tcacctgcat aggcttcttg gatttcgatt cttttcaacc ctaagaatcc
      721 atacattttc gtcactcgga agttttttgt tgatccatca cgttttagga ctgtcacttg
      781 ctggccgact ttcatcgtcc ctctaaatac acgtccaata ccgatacggc cgacataatc
      841 attataatcg agcaaggaca cttggaattg aagcggttct tcccggttat caatcggagc
      901 tggaatatgt tcaactatcg cgtcgaacaa tacttgcaac gtatcgtcct gatcagcagg
      961 gtccggtgaa aggctcgctg ttccgttaaa accggacgca tacacaactg ggaattcaag
     1021 ttgttcgtca tttgcatcca attcgatgaa taattcaagc acttcgtcaa ctacttcttc
     1081 gggacgggca aaatcgcggt cgattttatt gactacgacg attggtttta tattggattc
     1141 caatgctttt ttcaatacaa accgggtttg cggcatacat ccctcaaagg agtcgacgac
     1201 taagacgaca ccatcgacca ttcgtaagat ccgttccact tctccgccga aatcggcatg
     1261 gcccggtgta tcaagaatat tgattttcgt ttctttatat tcgaccgctg tatttttcgc
     1321 caggatcgta attccgcgtt cacgttctag atcatttgag tccatagccc gttcttcaac
     1381 atgttcattc gagcggaata ttccggattg ctttaaaagt tgatcgacta acgttgtttt
     1441 tccgtggtca acgtgggcga tgatcgctat atttcgtaaa tcattacgat tgtttgtcat
     1501 gttttcactc cgttatcttt tatcagatga ttaactgtgt tattatagca catgagaaca
     1561 gttttaaaaa acattttatt cgggagatgt ttcacattga aggatattaa atgggttttc
     1621 gtattctatt cccttgcagc ggtcgctgca atgtgtggaa tcggcatcgc agtcgctatg
     1681 cgtagtatcc ccgttgccat aattgctatc cttgctctta ttttcgtcat gggaaatggt
     1741 ttcaagacga aaaagaaaat gcgggaacaa ggtattttgt aatttcaact tatgaaaggg
     1801 actgcagtca tgcagcccct tttttattat ccagcttcgg gtggcagctg ctcgggtcat
     1861 aagtcagctc tgctgcacgg caaagatcgc cctttcgcag atccgcctta tgcctgtcac
     1921 agcttaccgc caccctccgc ttttgtttat ttgagtttaa tatagtttcg tagaatttcg
     1981 tcatgcagtc cgggccttgc gacgataaag gtatcctggc cgagcaaatg cggttgttcg
     2041 cctttcaaat tcgtagtgac tgcgccaact tcctgcgcaa tgactatacc ccctgcaatg
     2101 tcccacgggg atagccgcaa agataaatag gcgtcaatcc ggccggaaga aacatatgca
     2161 agctctatgg ccgctgagcc gtaagatctt gttcccctgc agtgacgcac catctctgca
     2221 atcggtccat tttcaattct tcggttgggc gcgacccagc tcgcatttac accaatgatc
     2281 gcctctgcaa gcggtgtttc ctcgagcacc ggcagtctct catcgttgaa ataagcccct
     2341 tcatctttaa ctgcgtgata aaaatcatca ttcatcacat catatatata tccgagcatg
     2401 ccgattccgt cagcgtaaat tccgagggag attgcaaaat tccttttctg gtggacgaaa
     2461 ttcattgttc cgtcaatcgg gtcaagcatc catataatgc catctagaga ttcgatttcg
     2521 tcaccgaatc cctcttcacc gaatattcgg tgtccaggaa agtccctctt gatccggctt
     2581 atgaaaaaac gctcgatctc ctgatcgata tttgtaacga gatcatttgc ttccgctttc
     2641 gaatcaacat cgatcgtact aaagaaagaa atccggattt tatgccctgc ctctttaatt
     2701 aaagattttg cataccggtc aattgctccc caattcatgt caacacctct tcatctgcat
     2761 gcatgttatt ttattatagc atatagccaa tacccaaaca aaaaggcgcc cttgcactgt
     2821 ccccccggtt cgaagagttt cagtgaaggc gcctaattcg gttcaacgac tttcgattaa
     2881 gcgaaagcat tgaaagtctc cagctcacca tgtatttcag tgagtctcgt tttgcttttc
     2941 tcgacttgtt tggagtcatt ttcctgtacg gcatcataca gtgtggccag ttcataatcc
     3001 aattcgagat gcagaagttt tacatactcc ttttcaattt cagctttgcg aataatatgg
     3061 acgacttgtt tcattactaa cacccctttg aatggtattt tctccaacga tgcatttact
     3121 tacttctaac ttatgctgtc cgggttgctt tggttagtta taagccttct tgtataagat
     3181 tgttccccat tttctgttac gataaacgtg gatattgaaa tggaggtatt aaattgacgc
     3241 aagggttttg gacaagtgat gattttaatg tcttttcaat tgagggatta gaacatagga
     3301 tgaatggcct tcaaacaaag atccagccga agtttcaggt gattggcgaa cagatttcga
     3361 tgtatttatc gtcccgtggg actggtgagt ttttccccca tgttgcaaaa catgcgagaa
     3421 ggacggtcaa tccgccgaat gacagctggg ttgcatttgc tccggctaag cgcggatata
     3481 aggcgctgcc ccatttccaa atcggcttat ggggttcaca tcttttcatc attttggcgg
     3541 tcatttacga gaatccggac aaaaaaggaa ttgcagagag gcttagccaa aaagttaatg
     3601 tgctaacttc cctacccgag gacttcgtcg tatcgggcga ccatatgaaa cccgaggcat
     3661 tcaggataaa tgatatcggt acagaaggtg tggaacgttt attagaaaga ttatcatcgg
     3721 ttaaaaaggc ggaattcatc atcggcaaac atctagtccg cgatgatgcg actacacttt
     3781 caaaaaagga attctttaac ttcgcagaag aaacgattga tagactcttc cctatttaca
     3841 acattatcat tggcaggtaa aaaaacagag cggatgcata attatgcatc cgccttgttc
     3901 atttgaaact gatgtaatct ccatcgctgc cttcttttgc tttcttgacg atccgataac
     3961 tgtttaatcc gctcgcctct tcaaattcct tgaagattgt cttctcctcc gcaatggaag
     4021 ggacaacttt cttgaatttc ttataggcag ccatgaaagt cgacctctcg atttgtgctt
     4081 catatgcttt ctctaccgct tgaaatagag cgaccacgtc aatgatttct tcggttgtcc
     4141 aatgatgact gagtggataa ttataatcca atttcttcat cgcctttcct tatgaccatt
     4201 ttcttctgat gtcttccgct tgctttacca ttcgaacgat caattccgct acagatggta
     4261 catcttcaat catgccggca acttgccccg cccagccaaa gccgctttgc tcatctccat
     4321 tgtggatgaa ccttcgattg gcgacaccgc taatatagtc ctttaattta tcataatcag
     4381 cattttccgc ttccatacgt aaaatctttt ccgtccaact tcccgaaagc gcccttgctg
     4441 gagcaccgag tgaacgttta ataacaacgg tacccgtttc ttctgtttga agtagtgcct
     4501 gtttgtattc ctccgaagca tgcacacatt cttttgtggc gatgaaacgc gtacccattt
     4561 cgatcccttc agctcccaat gcgtgcgccg ccatccaccc ccgtccgtct ccgatgcctc
     4621 cggacgcgat cacaggaatt ttcacagcat cgaccacttg gggaactaag accattgtgc
     4681 cgatatcatc acggcccaga tggcctcctc cctcctgccc gacgaccatc actgcgtctg
     4741 cgccgagcga ttcagctttg accgcctgcc ttttggcagc gaccaatacc agcttcttca
     4801 cgtccgcatc cccaacataa tcgaagaaag gcgcaggatt gcctcctgtt accgaaagta
     4861 cctttacctt ttcctccaat gcgacatcca atagatgttc atacggccgt ccgtgctgac
     4921 cgattgcaaa gttgactccg aaaggcttcg ctgtcagctc cctgacct
//
LOCUS       DASULI010000227         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1063268,
            whole genome shotgun sequence.
ACCESSION   DASULI010000227 DASULI010000000
VERSION     DASULI010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063268"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VFX34_04995"
     CDS             <1..428
                     /locus_tag="VFX34_04995"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766344.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX5564292.1"
                     /translation="LRIDVATKGIRNGYLMAVAPNSSTSVIAGSTASIDPIFKAFYHE
                     EKKDYKLPVIAPDLDHNTYDVYRRSAYIVDQRWSIKQNAARQRHIDQAISFNVYVPNN
                     IRASVLLDLHIQAWKSGMKTTYYMRSTASDIEECEWCHS"
     gene            413..862
                     /locus_tag="VFX34_05000"
     CDS             413..862
                     /locus_tag="VFX34_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX5564293.1"
                     /translation="MVSFLIVYASWSGNTEEVAEIVEETLLSEGMDVAVHRIGIGPMP
                     NPGEFDAMVIGSFTWDKGSTPDEVKDFVADVGYKPRNVYVFGTGDTQFGGDELFCGAA
                     VKLAKFYHSTYEPLKIEQSPRGMQEKKVIEWSKGVVQHWQHLHALKY"
     gene            808..1869
                     /locus_tag="VFX34_05005"
     CDS             808..1869
                     /locus_tag="VFX34_05005"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010648524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX5564294.1"
                     /translation="MVERSSSTLATLTRVKVLNPAHPNKSTAIFGGEASGILNWNDLA
                     HPHFYTLRQRIRSLFWTANEVDMTQDVKQFPSLSQAEQKAFLKIIGLLATLDGPQTVI
                     AMKIADFTTDPSVKSIMATIADQESEHNHSYAYVLSSVTTLDKQMASFEMGRTDEVLM
                     KRNERIVDIYNEFAENPTIETVLKAMVYTTLLEGLFFYSGFAFFYNLARHQKMVGTST
                     MISYINRDELQHGKAISDIFRAALAENPEYNTEAFTEWIYDQFRHSVEQEIIWSRYVL
                     GEIDGIDLDEMAGYVKYRANKMLRMLGLSEIYPEYTDNPMKWIRAYVDNFDDTKTDFF
                     EQTSRQYVKTSDLNGFDDL"
     gene            complement(1918..>2202)
                     /locus_tag="VFX34_05010"
     CDS             complement(1918..>2202)
                     /locus_tag="VFX34_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-alpha domain-containing protein"
                     /protein_id="HEX5564295.1"
                     /translation="MPILVQNTGYTGFYFRVLKEGIVSPSDELVHISRPSLAITVTEA
                     NRLMHHDRDDLEGIRNLLKVDELSESWRKTFEKRLAGNKADTSERLTGSK"
BASE COUNT          589 a          504 c          569 g          540 t
ORIGIN      
        1 agctgcgtat cgatgttgcg acaaagggta ttcggaacgg ctatttgatg gcggttgcgc
       61 cgaacagctc gacgtctgtc attgcagggt caacggcttc catcgatccg attttcaagg
      121 cgttctatca tgaagaaaag aaagactata agcttccggt cattgcgccg gatcttgatc
      181 acaacactta tgatgtgtac cgccgctccg cgtatatcgt cgatcagcgc tggtcgatca
      241 agcagaatgc ggcaaggcag cgccatatcg accaggcgat ttcgttcaac gtctatgtac
      301 cgaataacat ccgcgcgtcc gtcctcctcg atttgcatat ccaggcatgg aaatcgggca
      361 tgaagacgac gtattatatg cggtcgaccg cttccgacat cgaggagtgc gaatggtgtc
      421 attcctgatt gtgtatgcat catggagcgg caatacggag gaagtggcgg aaatcgtgga
      481 ggagacgctg ctgtcggaag gaatggatgt ggctgtgcat cggatcggga ttggtccgat
      541 gccgaatccg ggcgagttcg atgcgatggt catcggttca ttcacatggg acaaggggtc
      601 cacgcctgat gaggtgaaag attttgtcgc ggatgtcggc tacaagccaa gaaatgtgta
      661 tgtcttcgga accggggata cgcagttcgg tggagatgaa ctattttgcg gtgccgcggt
      721 caaactcgcg aaattttatc actcaacata tgaacctttg aaaatcgagc aaagtccccg
      781 tggtatgcag gagaagaaag taattgaatg gtcgaaagga gtagttcaac attggcaaca
      841 cttacacgcg ttaaagtact gaatccggca catccgaata aatcaacagc gattttcggc
      901 ggggaagcaa gtggcatcct gaactggaat gatcttgccc accctcattt ctacacattg
      961 cgacaaagga tccgttccct tttttggacg gcgaatgaag tcgatatgac gcaggacgtg
     1021 aagcagtttc cgagcttgtc gcaggccgag cagaaggctt tccttaaaat catcggattg
     1081 cttgcaactt tggatggccc tcaaaccgtc atcgctatga agattgcgga tttcacaaca
     1141 gatccttcgg tgaaatcaat tatggcgaca attgctgacc aggaaagcga acataatcac
     1201 agttatgcgt atgtattgtc gtccgtcaca acgctcgaca agcagatggc ttcgtttgaa
     1261 atgggccgca ccgacgaagt gctcatgaaa cggaatgagc ggattgtcga catctataat
     1321 gaattcgcgg agaatccgac gattgaaacg gtattgaaag cgatggtgta cacgacatta
     1381 ttggaagggc tgttcttcta tagcggcttt gcatttttct ataatctcgc acgccatcag
     1441 aaaatggtgg gcacgtcgac aatgatttcc tacataaatc gggatgaatt gcagcacggc
     1501 aaagcgatca gtgacatttt ccgcgccgca cttgccgaaa accccgagta taatactgaa
     1561 gcttttacgg agtggattta tgatcaattc cgccactccg ttgaacagga aatcatttgg
     1621 agccgctatg tgctaggaga gatagatgga atcgacctgg acgaaatggc aggctacgta
     1681 aaatatcgcg caaataaaat gctgcgcatg ctaggactta gcgaaatcta cccggaatac
     1741 acggacaatc cgatgaaatg gatccgcgcg tacgtcgata acttcgacga tacgaaaacg
     1801 gatttctttg agcagacgtc gaggcaatat gtgaaaacga gtgatttgaa cggatttgac
     1861 gatttataag aatgaaaaga acgctggcta ttcgacgtgg ccatgcgttc ttttttatca
     1921 tttgctcccc gtcaaccgtt cgctcgtatc cgctttattg cccgctagcc gcttctcaaa
     1981 ggtttttctc cagctctccg acagttcgtc taccttaagc aaattccgga ttccttccaa
     2041 gtcatctctg tcatgatgca taagccgatt tgcctccgtc acagtgatgg ccaaggacgg
     2101 tcgcgaaata tgcaccaact catcggaagg ggagacgatg ccttccttta acacacggaa
     2161 ataaaaacca gtatagcccg tgttttgcac aaggatgggc at
//
LOCUS       DASULI010000228         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1280021,
            whole genome shotgun sequence.
ACCESSION   DASULI010000228 DASULI010000000
VERSION     DASULI010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280021"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VFX34_05015"
     CDS             <1..356
                     /locus_tag="VFX34_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564296.1"
                     /translation="DIELILLCIGILLIILSFFIGKSTRKNEEEIENISISLHQETNQ
                     LKKRLKAVEEELMIGVGPALNGQKRKTKPVHEIIVNQILSLKAQGYSIPEIAKRASLP
                     DEEVVDVLRSRGVAL"
     gene            356..838
                     /locus_tag="VFX34_05020"
     CDS             356..838
                     /locus_tag="VFX34_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564297.1"
                     /translation="MKDILRGVGIGCILAGGILYFTNNGQTPSDDEQYKTQLDELQRE
                     LDKVKKELAVAQTLSSSNSGNTAGQDSKASDKETETKPDIEPASKPITKIILTIESGS
                     TSATVADKLERAGIIDSAKGLEQYLIDNGLAGRIQIGVHEVDTTMDLGTIARIITNTK
                     "
     gene            complement(884..3079)
                     /locus_tag="VFX34_05025"
     CDS             complement(884..3079)
                     /locus_tag="VFX34_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5564298.1"
                     /translation="MKKPMRKVDQAKIRQRKHTAFRMNLLFFSIFILFSLLIFRLGYL
                     QIVKGEKYTRLLEQKEEIAVNTSVPRGRIYDRTGKVLVDNKPMNAITYTKTSSTTAHD
                     MLEIAEGLSELIHQDTKRVTIGDKRDFWILLHPEEAEAKVSAEEKLEIGKDGTVSKQD
                     IQREVTRLTRERITDEELNSFTDHELEVLAIYREMMSGYAYSPQIIKSGNVTEEEFAA
                     VSERLSEFEGVDTTTDWDRIKFSDSTILGTMTSPIEGIPRSHLDYYLARDYSRNDRIG
                     RSYFEQYYEELLRGQKTIVKNIKDRTGQVIETKTVREGEPGRDLVLSMDSELQESLEE
                     LLSEKLLEMKKDPRSGLLDRAFLVMMDPNNGELLSLVGKRLVKDEDTGRWEVRDYAYG
                     TFTSAYEVGSTVKVATVLAGYSEGVLSVGDVKIDEPMNIAGQNKRSLFNQHGRYAIDD
                     ITALGRSSNVYMFKIAISIGKGVYRPYKALPIDIAGFDRLRNAYASFGLGVKTGIDLP
                     GEFAGIPGSDTRSGKLLDFAIGQFDTYTPLQLVQYISTVANGGNRVAPKVLKEIREPS
                     PDGETLGPLLEETEVKVLNRINNTQKEIEQVKKGMKYVYYGPNGTAPNLFKDASYTAA
                     GKTGTAQSFYYGDDRSKYNMASVNLTHVGFAPADNPEVAYAVVIPYASTDRGNYRTQG
                     RAMLDIVRTSLDTYFELKEERAKTNAPETTDQKIDRRFEREKENETNKK"
     gene            complement(3185..3787)
                     /gene="sodA"
                     /locus_tag="VFX34_05030"
     CDS             complement(3185..3787)
                     /gene="sodA"
                     /locus_tag="VFX34_05030"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HEX5564299.1"
                     /translation="MAYKLPELPYAYDALEPHIDKETMNIHHTKHHNTYVTNVNNALE
                     GHDDLAAKSVEELISDLDAVPENIRTAVRNNGGGHANHTFFWNILSPSGGGNPTGALA
                     EAIDKKFGSFDAFKEEFAKAAATRFGSGWAWLVSNNGELEIMSTPNQDSPLMEGKTPI
                     LGLDVWEHAYYLNYQNRRPDYVSAFWNVVNWDEVAKNFGK"
     gene            complement(3872..>4123)
                     /locus_tag="VFX34_05035"
     CDS             complement(3872..>4123)
                     /locus_tag="VFX34_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX5564300.1"
                     /translation="GSKAGMWGSTIGLLVGPFVIPVAGILIGPFLGAFLSELLVTRTG
                     VKQSLKTGIGSIVGFFTSVAAKGVVMLLMIGVFILYVAI"
BASE COUNT         1090 a         1009 c          854 g         1170 t
ORIGIN      
        1 tggatattga attaatttta ctatgcatcg gcatactact catcatttta tcatttttca
       61 tcggaaaatc cacccggaaa aacgaagagg aaatcgaaaa catttccatc agtcttcacc
      121 aggagacaaa tcaattgaaa aagcggttga aagcggttga agaggaattg atgatcggtg
      181 tcggccctgc actgaacgga cagaagcgaa aaactaaacc tgtccacgaa atcatcgtca
      241 atcaaatctt gtccttgaag gcgcaaggtt attcaattcc tgaaattgcc aaacgcgctt
      301 ccctaccgga cgaggaagtc gtcgatgtct tgagatcccg aggtgtcgca ctatgatgaa
      361 ggacatttta cggggagtcg gaataggctg catcctcgca ggcggcatcc tttatttcac
      421 gaataatggc cagactccgt cggatgatga acagtataaa acccagttgg acgagttgca
      481 aagagaattg gataaggtga aaaaagagct ggcagtcgcc caaacgctat cttcatcaaa
      541 ttccggaaat accgctggcc aagattcgaa ggcaagcgat aaagagacgg aaacaaagcc
      601 tgacatcgag ccagcttcaa aacctattac aaaaataatt ttaacaatcg aatcaggttc
      661 aacatctgca actgtagccg ataagcttga acgcgcaggg atcatcgaca gtgcgaaagg
      721 attggaacaa tacctcattg acaacggctt agcaggacgg attcaaatcg gcgtgcacga
      781 agttgacaca acgatggacc ttggcacaat cgcccgcatt ataacaaata caaaataaag
      841 aaaaaaagct gcccttcgcc gattatcgcg atggcagctt ttctcatttc ttattcgtct
      901 cattttcttt ttcccgttca aatcggcggt caattttctg atcggtcgtt tcaggggcat
      961 tggtcttcgc ccgctcttcc ttcaactcaa aataagtatc taaagatgtt ctgacgatat
     1021 caagcattgc tctcccttga gtacggtaat tgcctcgatc tgtcgaagcg tatggaatga
     1081 caacggcgta tgcaacttcg gggttatcag ctggcgcaaa gccgacatgc gttaaattta
     1141 cagatgccat gttatattta cttcgatcat ctccgtaata aaaggattgg gcagtacccg
     1201 tcttccctgc cgccgtataa gaagcatctt tgaaaaggtt tggcgcagtc ccattcggtc
     1261 cgtaatacac gtatttcata ccttttttga cctgttcaat ctctttttgc gtattattga
     1321 tgcgattaag gacttttact tccgtttcct ctagcaaagg tccaagtgtt tcaccatccg
     1381 gggatggttc ccttatttcc ttcaaaactt tcggagcaac acggtttcct ccattcgcta
     1441 cagtcgatat atattgtaca agctgtagcg gggtgtacgt atcgaattgg ccgatggcaa
     1501 agtcaagcaa cttaccggaa cgcgtatccg aacctggaat gcccgcgaat tcaccaggca
     1561 aatcgatccc tgttttcacc cctaagccga acgaggcgta tgcatttcgg agcctgtcaa
     1621 agccggcaat gtctattgga agcgccttat aaggtctgta tacgccttta cctatcgaaa
     1681 tggcaatttt gaacatataa acgttggaag accttccaag cgccgttata tcatcgatgg
     1741 cataccgtcc gtgctgattg aatagggatc ttttattctg accggcaata ttcatcggtt
     1801 catcgatttt cacatcacca accgacagga ccccttcact atagcccgcc aaaactgttg
     1861 ccaccttcac agtggaacca acttcatagg cggaagtgaa ggtcccatac gcataatccc
     1921 gcacttccca tctgcccgta tcctcatctt tcaccaatcg ttttccaact aatgaaagca
     1981 attcgccatt attcggatcc atcatgacaa ggaatgccct atccaacaat ccagatcgcg
     2041 ggtccttctt catttcaaga agcttttcgg aaaggagttc ctccaatgat tcctgcaatt
     2101 cactatccat tgataggaca aggtctctgc ccggctcccc ttccctgact gttttcgttt
     2161 cgatgacttg gccggtccga tctttaatat ttttgacgat tgttttttgt cctcttaaca
     2221 gttcttcata atattgttcg aaatagcttc ggccgatcct gtcattccgt gaataatccc
     2281 gcgccaaata atagtctaga tgactgcgtg gaataccttc gatcggactt gtcatcgtac
     2341 ctaaaatcgt actgtcggaa aatttaatac ggtcccaatc ggttgtcgta tcgactcctt
     2401 caaattcgct caatctttcc gatacggcag cgaattcctc ctcagtgaca tttccgctct
     2461 taatgatttg tggcgaatac gcatagcccg acatcatttc ccgatagatg gccaacactt
     2521 caagctcatg gtccgtgaag gaattcagtt cttcatccgt aatcctttca cgtgtcaaac
     2581 gagtcacttc gcgttggata tcctgttttg aaacagtgcc gtctttgccg atttcaagtt
     2641 tttcttcagc acttactttc gcctccgctt cttccggatg caataaaatc cagaaatcac
     2701 gtttatcacc gatcgtgact cttttcgtat cctgatggat caattcggat aggccttccg
     2761 cgatctccag catgtcgtgt gccgttgtag aagaagtttt cgtatatgtt atggcattca
     2821 taggtttatt atcgacgagg acctttcctg tccggtcata gatccttccg cgaggaacgc
     2881 ttgtattgac cgcaatttcc tctttctgct ccaatagcct cgtgtacttt tcgcctttga
     2941 cgatctgtaa gtatccaagt cggaaaatga gcaaggaaaa caggatgaaa atcgaaaaga
     3001 ataataggtt catcctgaag gcggtatgtt tccgttgtcg gatttttgct tggtccactt
     3061 tgcgcatcgg ctttttcata tgtattcccc ttacattttt agtccagatt gaattatagc
     3121 attctaaaca taaaaagcat atatcctgac gaacaggata tatgcttggt tgcattactc
     3181 tggattattt accgaagttt ttagcgactt catcccagtt tactacgttc cagaatgcgg
     3241 aaacgtaatc agggcgtctg ttttggtagt tcaaataata tgcatgctcc caaacgtcga
     3301 gtccaagaat aggagttttc ccttccatca gtggagaatc ttggttagga gtcgacatga
     3361 tttcaagctc gccgttgttt gatacgagcc aagcccaacc tgaaccgaaa cgagttgcag
     3421 ccgcttttgc gaattcctct ttgaaagcat caaagctgcc gaacttcttg tcgatcgctt
     3481 cagctagtgc tcctgtcggg tttccaccac cagatggtga aaggatgttc cagaagaacg
     3541 tatggttcgc atgacctcca ccattattgc ggacagccgt acggatgttt tcaggaacag
     3601 catccaggtc ggaaatcagc tcttcaacag actttgcagc caagtcgtcg tgcccttcca
     3661 atgcattgtt cacgtttgtc acatacgtat tgtgatgttt cgtgtggtgg atgttcatcg
     3721 tttctttgtc gatgtgaggc tccagcgcat cgtaagcgta tggtagttct ggtaatttgt
     3781 aagccatttg tccattcctc ccaattgatt cataatttat cctacactat aaacattatc
     3841 aaaattaggg aatccattca attgaaatga actatatcgc cacgtagagg atgaaaactc
     3901 cgatcatcag cagcatgacc acacctttgg cggctactga cgtgaagaag cctacgatgg
     3961 atccaatccc cgtcttcaat gactgtttca ccccggtacg cgtcacaagc aattccgaca
     4021 gaaaagcacc gaggaatggc ccgattagga ttcccgccac tggaatgacg aagggtccta
     4081 cgagtaatcc gatcgtactt ccccacattc ctgccttcga acc
//
LOCUS       DASULI010000229         4685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1093631,
            whole genome shotgun sequence.
ACCESSION   DASULI010000229 DASULI010000000
VERSION     DASULI010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093631"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFX34_05040"
     CDS             complement(<1..580)
                     /locus_tag="VFX34_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5564301.1"
                     /translation="MKDGKFALEDYIHFLKVERQLSQNTVASYKRDLSDYILQLEKAG
                     VESIDDIDRSAILQHLHAMKEQGKSSRTIARHISSIRSFHQFLLREKVTTQDPTVHLE
                     MPKLEQKLPSVLSVREVDKLISMPESSKPQGKRDIALLELLYGTGMRVSELIGLNVDD
                     IHISMGFVRVFGKGGKERIVPLGGGAMKACTTY"
     gene            complement(630..1088)
                     /locus_tag="VFX34_05045"
     CDS             complement(630..1088)
                     /locus_tag="VFX34_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX5564302.1"
                     /translation="MESRIERIKKQLHGASYKLTPQREATVLVLLENEEDHLSAEDVF
                     LLVKEKAPEIGLATVYRTLELLTELKVVDKINFGDGVSRYDLRQEGASRFHHHLICIE
                     CGKVAEIQEDLLGDVEKIVEGRYGFSVKDHWLTFHGICESCKSAGNDSKK"
     gene            complement(1742..2287)
                     /locus_tag="VFX34_05050"
     CDS             complement(1742..2287)
                     /locus_tag="VFX34_05050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5564303.1"
                     /translation="MNRFEEKTVASEKIFEGKIISLRVDDVELPDGKHAKRELVDHPG
                     AVAIIPITDEGKLILVEQYRKALNRSIIEIPAGKIEPGEDIEVTALRELEEETGYGAN
                     KFDYLQSFATSPGFANEIIHIFVAQELYTIDKPAAGDEDEFIQLYECTIEEAEQMVLD
                     QRIYDAKTAFAILHAKKLLKQ"
     gene            2393..3295
                     /locus_tag="VFX34_05055"
     CDS             2393..3295
                     /locus_tag="VFX34_05055"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5564304.1"
                     /translation="MKKREIGNSGMYVSELGLGCMSFPENLEEVKNIVDAALHAGINF
                     FDTADLYAAGKNESLVGEALKGRRNDIILATKVGNRMIPGEDGWVWDASKEYIMEAVK
                     DSLRRLGTNYIDLYQLHGGTMEDDVTETIEAFEALKKEGVIRQYGISSIRPNVIERFL
                     TNSSAVSVMMQYSLLDRRPEEWFPMILKAGASVITRGTLAKGLLTDEGPTRVRKMDGF
                     VAYDFEKLKSTVDKLHESANDVHALAQAFNLKEEVVASALIGASSKDQLLDSIVAYEK
                     AVEQSELASVAQLTEAHQYEQHRP"
     gene            3399..3629
                     /locus_tag="VFX34_05060"
     CDS             3399..3629
                     /locus_tag="VFX34_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564305.1"
                     /translation="MKLLTENKKWLKVGAATFLSVGLLAACADGEDDTDVDVNNPPAD
                     VDDGVNDGTDTDVDLDVDDDADTDTDTDDSSQ"
     gene            complement(3684..>4685)
                     /locus_tag="VFX34_05065"
     CDS             complement(3684..>4685)
                     /locus_tag="VFX34_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HEX5564306.1"
                     /translation="LIIVLISTFFGKLIKFFPPVVTGSVVTIIGITLIPVALNNMGGG
                     FGAPDFGSSTNIILSFGTLLFIILLYRFTTGFIRSISILLGMAAGTVAAMMFGIVDFS
                     AVNEASAFHLVTPFYLATPTFQLIPILTMTLVAMVSLVESTGVYFALSDICKKDINEK
                     DLAKGYRSEGLASLIGGIFNAFPYTTFSQNVGLVQMSGVKSRRVILITAIMLVSLGFM
                     PKIAAMATVIPTSVLGGAMVAMFGMVISQGIKMLSKVVGESQENAMIIACSVGLGLGV
                     SVVPDIFASLPASIQMLTSNGIVAGSLTAIVLNIMFNMLPSKKRKKAMQVQLTEQEV"
BASE COUNT         1383 a         1082 c          989 g         1231 t
ORIGIN      
        1 tgtaagttgt acatgctttc attgcgccgc cgcctaacgg cacgatcctt tcctttccgc
       61 cttttccgaa gacgcggaca aaccccatgg atatatgaat atcgtccaca ttcaaaccga
      121 tcagctcact gacacgcatt ccagtcccat aaagcaactc cagcaacgca atgtcccttt
      181 tcccctgtgg cttactgctt tccggcatgg aaatcaattt gtccacttcc cttacagaaa
      241 ggacagaggg caacttttgt tcaagcttcg gcatttccaa atggacggtc ggatcttgtg
      301 tcgtcacttt ttcccgtaat aggaattggt ggaatgagcg aattgaagaa atatgccttg
      361 caatcgtcct ggacgatttc ccttgctctt tcatagcatg caaatgttgc agaattgctg
      421 aacggtcgat atcatcaatc gactcgacac ccgccttttc caactggagg atataatcgg
      481 aaaggtctct tttataagaa gcaaccgtgt tttgcgacag ttgccgttcc actttcaaaa
      541 agtggatgta gtcttctaat gcaaacttgc catctttcat acatttcacc ccaaatgaca
      601 aaaagggaca gcatcacgcc atccccattt cactttttac tatcatttcc cgcagatttg
      661 cagctttcgc aaatcccatg gaaggtcagc caatgatcct tcacactgaa tccatatcgg
      721 ccttcgacaa tcttctcgac atcgcccaaa aggtcttcct gtatttcagc gacttttccg
      781 cattcaatgc aaataagatg atgatgaaaa cgcgatgccc cttcttgacg taaatcataa
      841 cgggagacgc catcaccaaa attaatcttg tcgacgacct tgagctccgt cagcagttcc
      901 aaagtccgat agacagtcgc aaggcctatt tccggcgctt tctctttgac aagtaaaaaa
      961 acatcttctg cgcttaaatg atcttcttca ttctcaagaa ggaccaaaac cgtagcttcc
     1021 cgctgaggcg tcaacttata gctggccccg tgcaattgct ttttaatccg ttcaatccgg
     1081 ctctccatat gacgcccccc ctcaacactg ataccattat accaaagagg cgttctttgt
     1141 gacaacagaa aggaaatagt agacttccag ccaatcagcg aatgagaaaa tggaatatcg
     1201 taatttccaa taaaaaaacg actaaagaaa atcccgccaa tacgggaagc gcttgaagat
     1261 tcctagcttt cctcgatact ttattgaaat aaggaggact taacactaca caatagataa
     1321 gaaatataag acaggaaatg aactggaacg ggaaccacca aattgcatat tcccatattt
     1381 tcattcctga atataagagg aaagaggatg acaatccaaa caaaacacat tttatcgcac
     1441 cgaaaatcaa aattgtatgc ctcgttttgc tgttcattgc cagaaggaat gtaacaataa
     1501 aaaacaacag cattattaaa aagggtctta atatagatcc atcggtggat gaaatgactc
     1561 tagggtcgaa aaagccgatc catttttcgg atgcagcaac cggaatttcc ctgaacaaca
     1621 atgccccgcc gatatatccg acggctagta tgataaatag atggataaaa gttaaggaac
     1681 ggaccacagg cttcacctcc gcatcgtttc tataacgtat gcttgtccgt cccaaaatag
     1741 ctcattgttt cagaagtttt ttcgcatgca ggatggcgaa tgccgttttc gcgtcgtaaa
     1801 tccgctgatc cagcaccatc tgttcagctt cttcgatcgt gcattcatac agttggatga
     1861 attcatcttc atcccccgca gcgggcttgt ctattgtata tagttcttgt gctacgaaaa
     1921 tatgaatgat ttcgtttgca aatccaggtg acgttgcaaa tgactgtaag tagtcgaatt
     1981 tatttgcccc gtagcccgtc tcttcctcca attcacgaag ggctgtcacc tcaatatctt
     2041 caccgggctc gatcttgcct gcaggaattt caatgatgga ccgattcaac gccttcctgt
     2101 actgctcaac aaggatcagc ttcccttcat cagtaatcgg aatgatggcg acagctcccg
     2161 gatgatctac gagttccctt ttcgcatgct ttccgtccgg cagttcgaca tcatcgacac
     2221 gaagggagat gatttttcct tcaaatattt tttcggatgc aacagtcttt tcctcaaaac
     2281 gattcattgt gacacgctcc ttttcgttaa aataagcaac aaacttcccc tatcaacttc
     2341 tacattgtac agtttatttt aagtggatga aaacaattac ggaggtgctc caatgaaaaa
     2401 aagggagatt ggaaatagcg gcatgtatgt atccgaactg ggcctcgggt gcatgtcatt
     2461 cccggaaaac ttggaagaag tgaagaacat tgtcgacgca gcattacatg ccggcatcaa
     2521 tttcttcgac acagcagact tatatgctgc tggcaaaaat gaatctctag ttggcgaagc
     2581 gttgaaaggt cggcgcaatg atattattct agcgacgaag gtcggtaacc ggatgattcc
     2641 gggagaagat ggatgggtct gggatgcctc gaaggaatat ataatggaag ccgtaaagga
     2701 cagtctgaga cgccttggca ctaactacat cgacctttac cagttgcatg gtgggacgat
     2761 ggaagatgat gtgacggaga caatcgaggc gttcgaagca ttgaaaaagg aaggggtcat
     2821 ccgacagtac ggcatttcgt ccatccgacc gaatgtcatc gaacgctttc taacgaacag
     2881 ttctgcagtt tccgttatga tgcaatacag cctgctggat cgacggcctg aagaatggtt
     2941 ccctatgatc cttaaagcag gagcgtccgt catcactcga ggcacattag caaaagggtt
     3001 gttgacagat gaaggcccaa ctcgagttag aaagatggac ggctttgtcg catatgattt
     3061 cgaaaagtta aaaagtacag tcgacaagtt gcatgaatcc gcaaatgatg tgcatgcgct
     3121 tgcccaagca ttcaatctga aggaagaagt ggttgcttcc gcattgatcg gcgccagctc
     3181 aaaggatcag cttcttgatt ccatcgttgc gtatgaaaag gcagtggagc aatccgagct
     3241 ggcctccgtt gcccaattga ctgaagcaca tcaatacgaa cagcatcgac cgtaagcttg
     3301 taatattttt gcaataaatc tgtaatgatt aggaaactat ttcaagggta tctaacaagt
     3361 aggcaacaat tatagattac accttggagg agatttcaat gaaactactt acagaaaaca
     3421 agaaatggtt gaaggttgga gcggctacgt tcctttccgt cggacttctc gcagcttgtg
     3481 cggatggcga agatgatacg gacgtagatg tcaataatcc acctgctgat gtagatgatg
     3541 gtgtcaatga tggcactgat acagatgtcg atcttgatgt agacgatgat gcagatactg
     3601 acacggatac agacgatagc agccaataag agattgaaaa acgagactcc gctatggagt
     3661 ctcgtttttt gttctgtata agattatact tcttgctcag tcaactgaac ttgcattgcc
     3721 ttcttacgct ttttcgaggg cagcatattg aacatgatat ttaaaacgat tgccgtcaaa
     3781 ctgccagcta caatgccgtt gcttgttagc atctggatgc ttgcaggaag gcttgcgaaa
     3841 atgtcaggaa cgacggagac tccaagtcct aggcctaccg aacaagcgat gatcatcgcg
     3901 ttttcctgtg attcacctac gaccttgctc aacattttga ttccttgaga aataaccatg
     3961 ccgaacattg ccaccattgc gccacctaaa acagacgtcg gaatgacggt tgccatcgca
     4021 gcgattttcg gcatgaagcc caatgagacg agcatgatag ctgtaataag aatgactcta
     4081 cgagatttaa cgcctgacat ttgtaccaaa ccgacgtttt gagaaaatgt cgtatagggg
     4141 aatgcattaa aaatcccacc gatcaacgaa gccaaccctt cggaacgata gcctttcgcc
     4201 aaatctttct cattgatgtc cttcttacaa atgtcgctta atgcaaagta gacgcctgta
     4261 gattccacta gcgaaaccat cgcgaccaac gtcatcgtta agattggaat aagttgaaac
     4321 gtcggtgttg cgagatagaa tggcgtaacc aaatggaatg ccgaagcttc gttcactgca
     4381 ctgaaatcta caataccaaa catcatcgct gctactgtac cggcagccat cccgagtagt
     4441 attgaaattg atctaatgaa accagttgta aaacggtata aaagaataat aaatagcaat
     4501 gttccaaatg agagaatgat gttagtggat gaaccgaaat caggcgcacc aaaacctccg
     4561 cccatattgt tcaaagccac aggtattaac gtaattccga tgattgtaac tactgatcct
     4621 gttacgactg gcgggaagaa tttaattaac ttcccgaaaa acgtactaat aagaacgata
     4681 attag
//
LOCUS       DASULI010000230         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1888100,
            whole genome shotgun sequence.
ACCESSION   DASULI010000230 DASULI010000000
VERSION     DASULI010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888100"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..3130)
                     /locus_tag="VFX34_05070"
     CDS             complement(74..3130)
                     /locus_tag="VFX34_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5564307.1"
                     /translation="MKVLEFIVKKKVLVGLLTVLIVALGSFAVVKLDKEIMPSAGMDG
                     AFVHIDAGDMAAVDMERFITSPLEQKLAGIEGVEEVRSTTTVGSSSLNITIARGQSEK
                     VMKEVDIAVSETKAERPEIREAMASQYGVQQGYEFFMDLSGDDLQAMTAFARDVLEPR
                     LEGLKEVHDVHLSGVSEYEVSIAFKRDEMKKHGLDISQVAGIIAQANSEATLGELQDG
                     KNASLLRWNSKLTDIDGVKRIEIPAAEGVIQLKDIADVTLAPLENSSFVWKNGTKELV
                     FIQVGRAGNITQIDMAKAVRAEIQKIRDEGLVKGFTLNEMVAQADIVQESIDGVVVNI
                     LIGSIVAIAILFLFLRNVRATLIIAVSIPTSVLLTFLTVYVLGYSLNMLTLIGLGLGV
                     GMMVDSSIVILESIYRKREMGLAKFEAVLAGTKEVAGAVFASMLTTIVVFLPIGFIGG
                     GLGEFMIILAIVVAITLISSVLVAFTLIPALSENFLRYRGVRDARKENRFGNGYNAVV
                     SWIVGKKRRCVLVLAVFFGLFAGSLFLVNKIPMTVMPDVFNRYSEIVVALETGVEPAE
                     KERLAHLMNDKLADIEDVESSYVLDGSGNQLFVIVNMTKGEDVVLEQKEVNEQILRSL
                     REFQDSEPIRAVQSAMSEVSGAPVQVMIKGDDFEQLQAIAGDFMGQLGKVDGIVAVTN
                     SMERTSEEKRVVLNEKAIADAGLSQMQVKQFIEQAFLEMPVGQMMLEGENVPLMVSWG
                     TKTDTQGALFELEIPTMEGDKKLSDLLSLEAVRTPNEITHIGGERFITVSADIEGRDL
                     GAINRDVQNVISEFNAPAGYAIDVAGDMEQQQQLMIEMLLVLGIALFLVYFVMAVQFN
                     HLGHPLIVMSVIPMAVTGVILGLFVTQMELNLLSGMGIIMLIGIVLNNAILLIDRTNQ
                     LRAEGMSVQEALVEAGRNRIRPIFMTTLTTVGGMLPLALASGASGNYQAPLATVLISG
                     LLFATFITLLLIPSVYRLFTKTENVAEAVTAKRKRRVATRLAEPVK"
BASE COUNT          837 a          994 c          672 g          829 t
ORIGIN      
        1 gtcttacttg tctgatacaa gcggaaaaac acgatctcca ttctccatga agatcgtgct
       61 cgcactaccc tattcatttt acaggttcag ccaatcgtgt cgccacgcgt ctctttcgtt
      121 tggcggtcac cgcctccgcc acattctccg tcttcgtaaa caaacgatac accgatggaa
      181 tcaatagcag cgttatgaac gtcgcaaaca gcaatccgga aatcaacacc gtagcgagcg
      241 gcgcctggta attgcccgat gcacccgaag caagcgcaag cggcagcatt ccgccgaccg
      301 tcgtcaacgt cgtcatgaag atcgggcgaa tccggttcct gcctgcctcg accaacgcct
      361 cctgtacaga catcccttcc gcgcgaagct ggttcgtacg gtcaatcaag agaatcgcgt
      421 tattcaacac gataccgatc agcatgatga tgcccatccc ggataacaga ttcagctcca
      481 tttgcgtcac gaacaacccg aggatgacgc cggtgactgc catcggaatg acggacatga
      541 caatgagtgg atgccccaga tgattgaact ggaccgccat gacgaaatag acaaggaata
      601 acgcgattcc aagcactaga agcatttcaa tcatgagctg ctgttgctgt tccatatcac
      661 cagcgacatc gattgcataa cccgcgggcg cattgaattc gctaatgaca ttttgcacat
      721 cgcgattgat tgcacctaaa tcccttccct ctatatccgc cgataccgtg atgaatcgtt
      781 ccccgccgat atgtgtgatt tcgtttggcg tgcgtacggc ttccaaacta agtaaatccg
      841 ataacttctt atcgccttcc atcgtaggaa tctccaactc gaatagcgca ccttgcgtat
      901 ccgttttcgt gccccaactc accataagag gcacattctc tccctccagc atcatttgac
      961 cgacaggcat ttcaaggaaa gcctgctcga tgaattgctt cacctgcatt tgtgataagc
     1021 cggcatccgc aatcgccttc tcattcagca ctaccctttt ctcctcggag gtgcgttcca
     1081 tcgaatttgt tacagcgacg attccatcaa ccttgccgag ctgccccata aaatcccctg
     1141 cgattgcctg cagctgctca aaatcgtccc ctttgatcat cacttgaaca ggcgctccgc
     1201 taacttcgga cattgccgat tgaaccgccc gtatcggttc cgagtcctgg aattcacgca
     1261 acgatcgtaa tatctgttca ttcacttctt tctgctcaag taccacgtct tcaccttttg
     1321 tcatattgac gatgacaaac agttggttcc cgctaccgtc caatacatag ctggactcga
     1381 catcctcaat gtccgccaac ttgtcgttca ttagatgtgc gagccgctct ttttcggcag
     1441 gctccacccc tgtttccagc gcaacgacga tttcactgta ccggttaaag acgtccggca
     1501 tgacggtcat cggaatttta ttcactagga aaagcgatcc ggcaaacagt ccgaaaaaga
     1561 ctgcgagcac tagaacacac cgacgttttt tcccgacaat ccaagaaacg accgcattgt
     1621 atccattacc gaaacggttc tccttcctag catcgcggac gccacggtag cgaagaaaat
     1681 tctccgaaag tgcagggatc agcgtgaatg caacgagcac cgagctgatc aacgtaatcg
     1741 cgacaacaat cgccaggatg atcatgaact cccctagccc cccgccgata aaaccgatcg
     1801 gcaggaatac gacgattgtt gtcagcatgg acgcgaaaac tgcgcccgcc acttcctttg
     1861 tccccgcaag tacagcctca aacttcgcaa gacccatctc ccgtttccgg taaatcgatt
     1921 ccaaaataac gatcgacgaa tccaccatca tcccgactcc aagtcccaga ccgatgagcg
     1981 tcagcatatt aaggctgtag ccgagcacgt ataccgtcaa gaaggtcaac agaaccgacg
     2041 tcggaatcga taccgcgatg atcagcgttg cacggacatt tcgaaggaat aaaaacagaa
     2101 tcgcaatcgc cacaatgctg ccgatcagga tattgacgac gaccccatcg atcgattcct
     2161 gtacgatgtc cgcctgtgcc accatttcat tcagcgtaaa gcctttcaca agcccttcat
     2221 cacgtatctt ctggatctcg gcacgaaccg ctttcgccat atcaatctgc gtaatgttcc
     2281 cagcccgccc tacttggatg aacaccaatt ccttcgtccc gttcttccat acgaatgacg
     2341 aattttcaag aggcgctagc gtaacatccg caatgtcctt cagttgaata accccttccg
     2401 ctgcaggaat ttcaatccgc ttcactccgt caatgtctgt aagcttcgaa ttccagcgta
     2461 gcagtgaagc attcttgcca tcctgcagct cgccaagcgt cgcttccgag ttcgcttgcg
     2521 caatgatccc tgcaacttgc gaaatatcca gtccatgctt tttcatttca tcccgcttga
     2581 acgcaatgga cacttcgtat tccgacacgc cggacagatg gacatcatga acttccttta
     2641 agccctcaag ccttggttca agcacatctc tggcgaatgc agtcatcgct tgaagatcat
     2701 cgcctgataa atccatgaaa aattcgtatc cttgttggac gccatattga ctcgccatcg
     2761 cctcccgtat ttcagggcgc tccgcttttg tttcacttac cgctatgtcg acttctttca
     2821 tgaccttttc gctctgcccg cgggcaatcg ttatattcag ggaactactc ccgactgtcg
     2881 tcgtcgaccg tacttcctca acaccttcga tccctgcaag cttctgttcc agcggcgaag
     2941 tgatgaaccg ctccatatca actgctgcca tatcccctgc gtcaatatgg acgaatgcgc
     3001 catccattcc ggcggacggc atgatttcct tatccagttt aacaactgca aagctcccca
     3061 aagctacaat caacacggtc aataaaccga ccaatacctt ctttttcaca atgaactcca
     3121 ataccttcac aaccattccc ctttccggcc tcattgttca ttagcttaac aagtttttcc
     3181 acttcctata acgtgcttca gccggatttc ggctcggact ttggatgtag agagtttaga
     3241 gaagtggtat acttttgatt agtcagaacg gttcggggtg aaattatgaa tgtaaaagag
     3301 tcatccaact ttttattgca tgcaaaaagc aa
//
LOCUS       DASULI010000231         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1130572,
            whole genome shotgun sequence.
ACCESSION   DASULI010000231 DASULI010000000
VERSION     DASULI010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130572"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFX34_05075"
     CDS             <1..315
                     /locus_tag="VFX34_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEX5564308.1"
                     /translation="LSMGHGRTLLGLRKKEQILLIAEKTINEGLNVRQLEKLVHRINE
                     NVPRETKPEKKNIFLEEQESHLREYFGTNVTIKKSKNKGRIEIEFFTEEDLERILDLL
                     NE"
     gene            379..1086
                     /locus_tag="VFX34_05080"
     CDS             379..1086
                     /locus_tag="VFX34_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEX5564309.1"
                     /translation="MILFGSIVNACLIIIGALLGRFLSNIPERMKETVMYGIGLAVTA
                     IGIQMTFESTQILIVIISIVIGAIIGEWLNLDDKINGLGKWIEKKLPSKKEGPGIAQG
                     FVTATLIFVVGSMAIIGAIDSGLRNDHDVLIMKGIIDGFTSVILSSTLGIGVMISAVP
                     VFLYQGLITIFSTQISRFVPDELLQFFISEMTATGGLMIVGIGLNLIGITKIRVANLI
                     PGIAVVAVIVTVVHFLF"
     gene            complement(1055..1633)
                     /gene="yyaC"
                     /locus_tag="VFX34_05085"
     CDS             complement(1055..1633)
                     /gene="yyaC"
                     /locus_tag="VFX34_05085"
                     /inference="COORDINATES: protein motif:HMM:NF018564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HEX5564310.1"
                     /translation="MHTALSTDYDYRLHYNETGAVWKLSTYFLEQIPFHEDSLTFFCI
                     GTDRSTGDALGPLTGSHLAESLLFPFHVVGTLEKPLHALNLQEQLDERLAMDPSPFIV
                     AIDACLGRSDSIGHLLFHQGPLYPGKAVGKELPPVGNLSIKGVVNISGFMEQAVLQST
                     RLHLPFEMSRIISRSLQLAYGRFKTGNERLSL"
     gene            1967..2620
                     /locus_tag="VFX34_05090"
     CDS             1967..2620
                     /locus_tag="VFX34_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5564311.1"
                     /translation="MLKLLENTLSYVVYFSAILAVLQEFNIDVKGLLAGAGVLGLAVG
                     FGAQSLVKDVISGFFILFEDQFSVGDYVKIGTAEGMVEEIGLRTTKIKNFTGEIFILP
                     NGTITQVVNYSMKNSLAIVDVTIPFELGVEKVEKSIDIFLKSMSKDNPDLLGKPKLIG
                     AHDFTEDSVIERIIAETRPMRHFDIARNISMGIKKQLEQEGIEIPYPTLVSKSFNNQ"
     gene            2650..2856
                     /locus_tag="VFX34_05095"
     CDS             2650..2856
                     /locus_tag="VFX34_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEX5564312.1"
                     /translation="MAEKEFHLNDVVEMKKPHPCGTNAWKIIRMGADIRIKCEGCGHS
                     VMIPRNEFAKKMKKVLVKADAGQN"
     gene            2938..>4015
                     /gene="ychF"
                     /locus_tag="VFX34_05100"
     CDS             2938..>4015
                     /gene="ychF"
                     /locus_tag="VFX34_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002166369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5564313.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFCTIDPNVGIV
                     EVPDERLQKLTELVVPKKTVPTAFEFTDIAGIVEGASKGEGLGNKFLSHIREVDAICQ
                     VVRCFDDENITHVSGKVNPIDDIEVINLELILADMESVDKRLTRVSKMAKQKDKDAMA
                     EEPVLLKLKEAFENEKPARSVELTPEEYAIVKGMHLLTIKPMLYVANVAEDEIADAEE
                     NKYVKMVRDFAAKDNAEVIVVSAKVEEEMAELDDEEKAMFLEELGIKESGLDQLIKAS
                     YQLLGLATYFTAGVQEVRAWTFRKGMKAPQCAGIIHTDFERGFIRAETVSYDALVEAG
                     SMAAAKEAGKVRLEGKEYIVQDGDV"
BASE COUNT         1256 a          760 c          986 g         1013 t
ORIGIN      
        1 ctttcaatgg gacatgggcg cacattgctc ggcctccgaa agaaggaaca aattctatta
       61 attgctgaaa agacaattaa tgaaggattg aatgtccgtc aattggaaaa gctagtccat
      121 agaataaatg aaaatgttcc acgtgaaaca aaacccgaaa agaaaaacat cttcctggaa
      181 gagcaagaat cgcatttaag ggaatatttc ggcacgaatg tgacgataaa gaaatcgaag
      241 aacaagggta ggatcgaaat cgagtttttc acagaagaag accttgaaag aatcctggat
      301 cttttgaatg aataagcctg tacctatttt gcttgatgca aaataggtat tatttttaca
      361 gatggaagtg aaaaaacgat gatcttattc ggatcgattg taaatgcctg tcttatcatt
      421 atcggcgctt tgcttggccg ttttttatct aatatccccg aacggatgaa agaaaccgtc
      481 atgtatggaa tcggattggc ggtcacggcc atcggcatcc agatgacatt tgaaagtacg
      541 caaatattaa ttgtcatcat cagcatcgtt atcggtgcga ttattgggga atggttgaat
      601 ctagacgaca aaatcaacgg attaggaaaa tggattgaaa agaaactccc ttcgaaaaaa
      661 gaagggcctg gcattgcaca aggatttgtg acggcaacgc tcatcttcgt agtcggttcc
      721 atggccatta tcggagcgat agacagcggc ttgcggaatg accacgatgt gttgatcatg
      781 aaggggatca tagatggatt tacttctgtc atccttagtt ccacgcttgg tatcggtgtc
      841 atgatatctg cagttccggt gttcctttac caaggtctca ttacaatttt ctcaacacaa
      901 ataagccgct ttgtcccgga tgaactattg caatttttca tttctgaaat gactgcgaca
      961 ggcggcttga tgattgtcgg aatcggattg aacttaattg gcattacgaa aatccgggtg
     1021 gcaaatctaa ttccagggat cgctgtcgtc gctgttatag tgacagtcgt tcatttcctg
     1081 ttttgaatct cccataggct agttgaaggg aacgggaaat gatccggctc atttcaaaag
     1141 gcaggtgcag ccgggtgctt tgcaggacag cctgttccat gaaaccggaa atattgacga
     1201 cacctttaat ggacagattg ccgacaggag gtaattcctt accgactgct ttgccgggat
     1261 agagaggccc ttgatggaaa agtaaatggc cgattgaatc acttcttcct aagcatgcat
     1321 caatggcaac gataaaaggg gatgggtcca tcgcaagccg ttcatctaat tgctcttgta
     1381 aatttaacgc atgtaaaggc ttttccaacg ttccgacgac atgaaacgga aacaataaag
     1441 actcggctaa atgtgagccg gtcaacggtc ccaatgcatc accggtcgac cggtctgtgc
     1501 cgatgcaaaa gaatgtcagg gagtcctcat ggaaagggat ttgttcaagg aaatatgtgc
     1561 tgagcttcca aactgcaccg gtttcattat aatgaagacg gtaatcataa tcagtggaaa
     1621 gtgctgtgtg catgacaaaa ctccattctc atataatagt tcaataattg attgtgtagc
     1681 agtatacgcg gcttcatgag gatttatacg gaaaggcatg atacggatga caaacgcgct
     1741 aacagaaagt ataaaaaaac tgtttcttgg cgaagaaaca gagaaaatag gcaaatttat
     1801 tttctccgaa gaaacatggg tcggtttcat agttattgct atcaagattg cctttatcat
     1861 cgcggtctct tttattgttg tacaagtggg caagtatatt attcgaaaaa tctttagaat
     1921 taaattaaag ggacctattc ggaaagaaga gcgacgggaa caaacattgc tcaagttgct
     1981 cgaaaacacg cttagttatg tagtttattt ttcggcgata ctagctgtcc ttcaagaatt
     2041 taatattgac gtaaaaggac ttcttgcagg ggcaggggta ctcggcctcg ctgtcggttt
     2101 cggtgcccaa agccttgtga aggatgtcat ttcgggtttt ttcatccttt tcgaagacca
     2161 gttttcagtt ggagattatg ttaagatcgg cacagcagaa ggtatggtgg aagagatcgg
     2221 tttgaggaca acgaagatta aaaactttac cggggaaatc ttcattttgc caaatgggac
     2281 gattactcag gtcgtcaatt attcaatgaa aaattcattg gccatcgtcg atgtaaccat
     2341 tccatttgaa ctgggtgttg agaaagtgga gaagtcgata gatatatttc ttaaatcgat
     2401 gagcaaggac aatcccgatc ttcttggaaa gccgaaattg atcggtgccc acgatttcac
     2461 ggaggactcc gtcatagagc gtattattgc tgaaacaagg ccgatgcgcc atttcgatat
     2521 tgcaaggaac ataagcatgg ggataaaaaa gcagttggaa caggaaggaa tcgaaattcc
     2581 atatcctaca ctcgtgtcaa aatcatttaa taaccaatag gttagatgaa taagggagga
     2641 aagagataga tggcggagaa ggaatttcat ttgaatgatg tcgtggaaat gaaaaaaccg
     2701 catccatgcg gaacaaatgc ctggaaaatt atcaggatgg gtgctgatat ccggataaaa
     2761 tgcgaaggtt gcggccatag tgtgatgatt ccacgaaatg aatttgcaaa gaaaatgaaa
     2821 aaggtgctcg ttaaagcgga tgccgggcaa aattagacag gaacgccaac tattcttata
     2881 atagaagagt tgcaaatatg attatgaatt tgacctaaat gaaaggttgg gaaataaatg
     2941 gcgttaacag ctggtatcgt cggattgccg aacgtaggaa agtcgacact attcaacgca
     3001 ataacgaagg cgggagcgga ggctgccaac tatccgttct gtacaatcga tccgaacgtt
     3061 gggattgtag aagtgccgga tgaacgactc cagaaattga cggagctcgt cgtaccgaaa
     3121 aagacagtgc cgacagcttt tgaattcacg gatatcgcag ggattgtcga aggggcaagt
     3181 aaaggagaag ggctcgggaa taaattcctt tctcatatcc gcgaagtcga tgcgatttgc
     3241 caagtcgtcc gttgttttga cgatgaaaac attacacatg tatcaggaaa agtaaatccg
     3301 atcgacgaca tcgaagtcat caatttggaa ctgatccttg ctgatatgga gagcgtggac
     3361 aagcgtttga cacgcgtttc caaaatggcg aagcagaaag ataaggacgc aatggcagag
     3421 gagccagtat tgctgaaact gaaggaagct tttgaaaatg aaaagcctgc ccgctcggtt
     3481 gaattgactc cggaagaata cgccattgta aaaggcatgc atttattgac gatcaagccg
     3541 atgctttacg ttgctaacgt cgcggaagat gaaatcgccg atgctgaaga aaacaagtat
     3601 gtcaaaatgg tccgcgactt tgccgcaaaa gacaatgccg aagtaattgt cgttagtgca
     3661 aaagtcgagg aagagatggc tgaactagat gatgaagaga aagctatgtt ccttgaggaa
     3721 ttgggcatca aggagtcggg cctcgatcaa ctcatcaaag cctcatatca actattagga
     3781 ctcgccacat acttcacggc aggcgttcaa gaagtccgtg catggacatt ccgaaaaggg
     3841 atgaaggcac cacaatgtgc aggcatcatc cacacagact tcgaacgtgg cttcatccgc
     3901 gcagaaaccg tatcctatga cgctctcgta gaagccggct ccatggcagc tgcaaaagaa
     3961 gcgggcaaag tccgtctaga aggaaaagaa tacatcgtcc aagacggaga cgtca
//
LOCUS       DASULI010000232         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_639162, whole
            genome shotgun sequence.
ACCESSION   DASULI010000232 DASULI010000000
VERSION     DASULI010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639162"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFX34_05105"
     CDS             <1..714
                     /locus_tag="VFX34_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5564314.1"
                     /translation="EGDDFVINGSKVYITNASHAKHLALTAITGHENGKKEISAIIVP
                     TDAEGFTVIDNYEKMGLNASNTTELVLDNVRVPKENLLGKRGDGFKQFLVTLDGGRIG
                     IGAMAVGIAQAAFDRALRYSKERKQFGKALSEFQITQFKLADMALKIELARNMVYKAA
                     WLKDQGRPFTKEAAMCKLYASEIAMEVADQAIQIHGGYGYMKEYEVERYMRDAKLLEI
                     GEGTSEVQRMVIARQIGCQ"
     gene            850..1227
                     /locus_tag="VFX34_05110"
     CDS             850..1227
                     /locus_tag="VFX34_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5564315.1"
                     /translation="MKNNPVVPYILIFALGLGLIFFMSLYGIDQKKEIAAKDEEGKTE
                     ETTDGAASEDFDPEAFAQGKCIGCHGGDLTGGMGPSLHGLALSQDEIHDIIKNGKDGM
                     PPFGSQISDEQIDQLSEYILSLK"
     gene            1337..2035
                     /locus_tag="VFX34_05115"
     CDS             1337..2035
                     /locus_tag="VFX34_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HEX5564316.1"
                     /translation="MNSENLSTRLNAVASFVEEGATLADIGSDHAYLPCHLVKLGRIR
                     KAIAGEVVKGPFESALKNVRKKGLSDEITVRLANGLEAIHESDGVDTITIAGMGGPLI
                     ASILSDGNEKLRNVKRIITQPNIHASSIREWAVRNGWKIIDEEILKEDNKIYEIIVLE
                     KGQAEYDELEMTVGPYLLDKRSDVFIEKWKREADEWKRVLISLENAGNTNEINEKKEQ
                     LRKKIDLIGGVLTN"
     gene            2032..>2317
                     /locus_tag="VFX34_05120"
     CDS             2032..>2317
                     /locus_tag="VFX34_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5564317.1"
                     /translation="MKKVNGYEIITLFEQWSPKRFAEDWDPIGLHIGQLNRPVEKAIV
                     TLDVNEAVVDEAIEKGANLIIAHHPPIFSPLKHIWTDTPQGRLIEKCIKHD"
BASE COUNT          741 a          425 c          589 g          562 t
ORIGIN      
        1 gaaggcgatg attttgtcat caatggatcg aaagtatata ttacaaatgc aagccatgcc
       61 aaacatttag cgcttacagc tattactgga cacgaaaatg gcaagaagga gatcagtgcc
      121 atcatcgtac cgacggatgc ggaaggattc actgttatcg acaactatga aaaaatgggc
      181 ttgaatgctt ccaacacgac ggaattggtg cttgacaatg tcagggttcc taaagagaac
      241 ctccttggca agcgcggaga cggctttaag cagtttctcg tcacgttgga cggaggcagg
      301 atcggcatcg gtgcaatggc tgtagggatc gcccaggctg catttgatag ggcgttgagg
      361 tattcgaaag aacggaaaca gttcggcaag gcgctatccg aattccaaat tacacaattt
      421 aagcttgcgg atatggccct gaaaatcgag ttggcaagaa atatggtcta caaggctgca
      481 tggttgaaag accaaggacg tccgtttacg aaagaagccg ccatgtgcaa gctgtatgct
      541 tccgaaatag cgatggaggt tgccgaccag gcgattcaaa ttcatggcgg ctatggttat
      601 atgaaagaat atgaagtcga gcgctatatg agggatgcga aattgcttga aataggcgaa
      661 ggcacatccg aagtgcaacg gatggttata gcaaggcaaa tcggttgcca ataagaaagt
      721 ttgtccaaat tgtcacaaag tctgaaatgt atagggataa ctctttcagt tttcttgcgg
      781 atacagtaca atattagatg tgcactgtat acttttacag aataattcct ggtgaaggag
      841 ggtaattcga tgaaaaacaa tcctgtagtg ccttatatcc tgattttcgc gctaggatta
      901 ggacttattt tcttcatgtc cttatacggc atcgatcaga aaaaagagat cgctgcaaag
      961 gatgaggaag ggaaaactga agaaacgact gacggagcag catccgaaga tttcgatcct
     1021 gaggcctttg ctcaaggaaa atgtatcggc tgtcacggcg gtgacttgac cggtggaatg
     1081 ggaccgagcc ttcatggttt ggcattatcc caagacgaaa tccatgatat cattaaaaac
     1141 ggtaaagacg gaatgcctcc attcggtagt caaatctcgg acgaacaaat cgaccaattg
     1201 tcagagtaca tcctatcact taaataagaa cgggaagcca tcggggcgtt atgaattcgc
     1261 ctccgatggc tttttcaatt ctggcaaaga tcatgaagtt cccttacaat aaggatattg
     1321 gaaaggcggg gatctattga attccgaaaa tttatcaaca aggctcaatg cggtcgcatc
     1381 atttgtcgag gaaggcgcca ccctcgctga tattggaagc gatcatgctt atttgccttg
     1441 tcatttagtg aaacttggaa gaatacgaaa agcaattgca ggagaagtag tgaaaggacc
     1501 atttgaatct gcactgaaaa atgtccggaa gaaaggcctt tcagatgaaa tcaccgtccg
     1561 tcttgcgaac ggattggaag ccatacatga aagtgatgga gtcgatacga ttactatcgc
     1621 gggaatggga ggaccgttga tagcatccat tttaagcgat gggaatgaaa aactgcgaaa
     1681 cgtcaaacgg atcatcacac aaccgaatat tcacgcttca tcgatccgtg agtgggctgt
     1741 tcgaaatggc tggaagataa ttgatgagga gattctgaag gaagacaata aaatctacga
     1801 aatcattgta cttgaaaaag ggcaggcaga gtatgacgaa ctggaaatga cggttggccc
     1861 ttacctactt gataaaagat cagatgtttt catcgaaaag tggaagcgtg aagcagatga
     1921 atggaaaagg gtcctcattt ctttggaaaa tgcgggaaat acgaatgaga tcaatgagaa
     1981 aaaggaacaa ttacggaaga aaatcgattt gatcggaggc gttttgacaa attgaaaaaa
     2041 gtgaatggtt acgaaatcat tacccttttt gaacaatggt cgccaaaaag gtttgcagaa
     2101 gattgggatc cgattggatt gcatatcggt cagttgaacc gcccggtgga aaaagccatt
     2161 gtcacattgg atgtcaacga agcagtagtg gatgaggcaa ttgaaaaagg ggccaatttg
     2221 atcattgcgc accatcctcc aatttttagt ccgttgaaac atatttggac ggatacgcca
     2281 caaggaaggc taattgaaaa atgtattaaa catgaca
//
LOCUS       DASULI010000233         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1305905,
            whole genome shotgun sequence.
ACCESSION   DASULI010000233 DASULI010000000
VERSION     DASULI010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305905"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFX34_05125"
     CDS             <1..553
                     /locus_tag="VFX34_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003320451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5564318.1"
                     /translation="SVIVATFVSYFVVRQNSAKLDFIASIPLVVPGIAFGIALIQTFN
                     TAPLQLTGTAVLLIIAYTIRRLPYMVRSTMGSMRAIKQDIEEAAVNLGATPLTAAITI
                     VGPLMLPGIAAGSVLVFITVIKEASVSILLAPPEWAPMSLAIFQNILRAEYYSAAAMS
                     IVLVVLVLILQALANLIGKKQRL"
     gene            625..1401
                     /locus_tag="VFX34_05130"
     CDS             625..1401
                     /locus_tag="VFX34_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEX5564319.1"
                     /translation="MLKGFIFDLDGTIYLGDHIIDGAPEVIRALKARGDKVVFLTNKS
                     IANRKEYVEKLNRLGIEVELDEVINSNYITAHYLKTVMGEDDSAYVIGEEPLFEELAN
                     ENIKLADIPASASHVVLGWDRQFTYDKLNHAFQAWRNGAEIIATNPDRTCPVKDGEIP
                     DCGAMIGALEGATGEPVKMITGKPSSLMAEYVLDNVLGMSADQCFMVGDRLETDIKMA
                     NDAGIHSVLVLSGITTEGMIAGSEHRPELILNSVKDLITL"
     gene            complement(1396..1959)
                     /locus_tag="VFX34_05135"
     CDS             complement(1396..1959)
                     /locus_tag="VFX34_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEX5564320.1"
                     /translation="MSLVDMVESQMIASVKNEEDLQHALTSNSNIVFLLTGNLMTIKD
                     YIQQLRAADKHVFLHLDFIDGLSNSKNALNYVAEYWNPTGIITTKSHIVKMANEVGLQ
                     TIQRIFLLDWAAINKGIEMVKSCKPDAVEILPGIIPKVIDQLSRDLDFPIIAGGLISD
                     ISEVHAALEAGALAVSSGNPSMWNFDL"
     gene            2130..>2638
                     /locus_tag="VFX34_05140"
     CDS             2130..>2638
                     /locus_tag="VFX34_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5564321.1"
                     /translation="MKSFSFTGRRELLGRIENEPYDVIIIGGGITGTGIALDCVTRGL
                     KTLLIEMQDFAAGTSSRSTKLVHGGLRYLKQFEVGMVAEVGKEREVVYRNAPHVTHPE
                     WMLLPIYREGTFGKYSTSLGLKVYDFLAGVKKKERRRMLSVQEALRKEPLLNRKRLLG
                     AGYYVEYRT"
BASE COUNT          664 a          548 c          756 g          670 t
ORIGIN      
        1 aagcgttatc gtcgcaacgt tcgtatcgta tttcgttgtg aggcaaaatt cggcgaagct
       61 ggatttcatc gcttccattc cgttggttgt gccgggcatc gctttcggga tcgcgctgat
      121 ccaaacgttc aatacggcgc ctctccaatt gacggggaca gctgtgctat tgatcatcgc
      181 ttatacgatc agaagacttc cttatatggt ccgttcgacg atgggatcga tgcgggcgat
      241 caaacaggat atcgaagaag cagcggtgaa cctcggggca accccgctga ccgcggcgat
      301 tacgatcgta gggcctttga tgctgccggg gattgcagcc ggatccgtac ttgttttcat
      361 tacggtcatc aaagaggcga gtgtttcgat cttgcttgca ccgcctgaat gggcgccgat
      421 gagtttggcg atcttccaga acatccttcg ggcagagtac tactcagcag cggcgatgtc
      481 gatcgtcctc gttgtcttgg tgctgatctt gcaggcgcta gccaatctga tcggtaaaaa
      541 acaacggctt tgattggaag aatgaagtgc gggtgctagc ttgcagacaa ataatggttt
      601 aggcataaat gacttggagt gagggtgttg aaagggttta tctttgatct ggatggaacg
      661 atttatctgg gtgaccatat tatcgatggg gcgccggaag tgataagagc gttgaaagcg
      721 cgcggggata aagtggtgtt cctgacgaat aaatcaattg ccaatcgcaa agagtacgtt
      781 gagaagttga atcgtttggg aattgaagtt gagcttgacg aggtcatcaa ttcgaattat
      841 atcactgcgc attatttgaa gacagtgatg ggtgaggacg actcagccta cgtgatcggc
      901 gaagagcctt tattcgaaga actggcgaat gaaaatataa agctggcgga cattcccgcc
      961 agcgcatctc atgtcgtatt aggttgggat cggcaattca cgtatgacaa gctgaatcat
     1021 gccttccaag cctggcggaa tggggccgaa atcattgcga ccaatccgga tcggacgtgt
     1081 ccggtcaagg acggggaaat cccggattgc ggagcgatga tcggggcgct tgaaggcgcg
     1141 acgggcgagc cggtgaagat gatcactggc aagccttcgt cgttgatggc tgaatacgtt
     1201 ttggataatg tgttaggcat gagtgcggat caatgcttta tggtagggga tcgtctggaa
     1261 acggatatta agatggcaaa cgacgccggc atccattccg tccttgtcct gtccggcatt
     1321 acgactgaag gaatgatagc tggttccgag catcgacctg aactcatatt gaatagcgtc
     1381 aaagatctta taacattata agtcgaaatt ccacatggat ggattgcctg aagagacggc
     1441 caaggcgcct gcttcaagtg ccgcatggac ctctgaaata tcggagatga gcccgccggc
     1501 aatgatcggg aagtccaggt cacgtgatag ttggtcgatg actttcggga tgatgccggg
     1561 caggatttcc acagcatccg gcttgcacga tttcaccatt tcgatccctt tattaatggc
     1621 agcccaatcc aacaggaaaa tccgctgaat cgtttgtagg ccgacttcat tggccatttt
     1681 gacaatatgg cttttcgttg tgatgattcc tgtcggattc cagtattcgg cgacgtagtt
     1741 gagcgcattt ttggaattgg aaagcccgtc gataaagtcg agatggagaa acacatgctt
     1801 gtccgctgcg cgcagctgtt gaatatagtc cttaatggtc atgagattcc cggtaagaag
     1861 aaagacgatg ttggaattgg atgtaagcgc gtgctgcaag tcctcctcgt tctttacgga
     1921 ggcaatcatt tgggattcga ccatgtcaac gagactcaag tttattcatc ctttcaaaaa
     1981 taggaaatat actatctata ataccatagc caatagctag taaaagaata atttaatacg
     2041 gtcggagatt atgagagaag gccattacaa tccctgcatg gagcagggct attgtaatgg
     2101 tctttttcaa tgataggagc gttttacaga tgaaatcatt ttcatttacc ggcagacggg
     2161 agctgcttgg caggattgaa aacgagccat acgatgtcat tattatcggc ggcggaatca
     2221 ccggaacggg catcgccctg gattgtgtga caagggggtt gaagaccctt ttaatcgaaa
     2281 tgcaggattt tgcagccggg acgtcaagcc ggtcgacgaa gcttgtccat ggaggactcc
     2341 gctatttgaa gcagtttgaa gtcggcatgg tggcggaagt cgggaaggag agggaagtcg
     2401 tctatcggaa tgctccccac gtcacgcacc cggaatggat gctcttgccg atttaccgcg
     2461 agggtacgtt tggcaagtat tcgacttcgc tgggattgaa agtgtatgac tttctggcgg
     2521 gtgtgaagaa gaaagagcgc cggagaatgt tatcggtgca ggaagcgctg cggaaagagc
     2581 ctttattaaa taggaaacga ttgcttggcg cgggctatta tgtggaatat cggacgga
//
LOCUS       DASULI010000234         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1794915,
            whole genome shotgun sequence.
ACCESSION   DASULI010000234 DASULI010000000
VERSION     DASULI010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794915"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..683)
                     /gene="ilvN"
                     /locus_tag="VFX34_05145"
     CDS             complement(168..683)
                     /gene="ilvN"
                     /locus_tag="VFX34_05145"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5564322.1"
                     /translation="MRRVITVTVINQSGVLNRVTGLLMKRQFNIESITVGHSEQPGMS
                     KMTFIVHVEDERKIEQLVKQLQKQIDVIKVNDITEKAIVMRELALVKVVSPPHARSEI
                     NSIVEPFRATVIDSGKNVVTYQVTGAPEKIEAFIDLVKPYGIKELARTGATAFTREIQ
                     KTNSPQLSILK"
     gene            complement(680..2440)
                     /gene="ilvB"
                     /locus_tag="VFX34_05150"
     CDS             complement(680..2440)
                     /gene="ilvB"
                     /locus_tag="VFX34_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX5564323.1"
                     /translation="MSAEVQTKQAVMEMAPESTVEKQNNQPMDGSAVLIQGLKDQGVE
                     VIFGYPGGAVLPIYDALYKDPIPHVLARHEQGAIHAAEGYARVSGKPGVVIATSGPGA
                     TNLVTGITDAMMDSLPLVVFTGQVASTVIGTDAFQEADVIGITQPITKHNYQVQDVAD
                     LPRIIKEAFHIASTGRRGPVVVDIPKNVATEIFVTEEERNAEVNLPGYQPTTTPNYLQ
                     IQKAAEAIRFAHKPLILAGAGVLHGQAMEELKELVETKRIPVTNTLLGLGSIAGDHEL
                     FLGMAGMHGTYTANMAISECDVLINVGARFDDRLTGNLDSFAPHAKVIHIDIDPAEIG
                     KNVPTEIPIVADSKEALKALLKQEFHAPETEEWLDYLNGLQEEAPLWYNTDEHEILPQ
                     EAIQMIHRITEGDAIVTTDVGQHQMWAAQYYSLNNPDHWVTSGGLGTMGFGFPAAIGA
                     QLAKPDERVVAIVGDGGFQMTAQELSLLQEMRIPVKVVILNNGALGMVRQWQETFYDE
                     RYSQSLIPVQPDFVKLAEAYDLKGVRITKKEDAEQLFREALLSDEPVLIDCRVKMLEN
                     VYPMVAPGKGLQEMIGVKSE"
BASE COUNT          593 a          782 c          672 g          860 t
ORIGIN      
        1 tcattgatat cctggttgta atacatttta gccattgtaa tttcctcctc gattttgggt
       61 tttattttaa tttaactgtt gatttccact cagtgtggcg agaggtgatt cctgctcgct
      121 gcacttcttt tgcttcaatc aactagaatt ccaaatttca atagacttta ttttaaaatg
      181 gacaactgtg gcgaattggt tttttggatt tcacgggtga atgccgttgc tccggtcctt
      241 gccaactctt tgatgccata tggcttgacg agatcgatga atgcctcgat cttctcgggc
      301 gcgccggtca cttggtaggt gacgacgttt ttcccggagt cgatgaccgt tgcgcggaac
      361 ggttcgacga tgctgttgat ttcactgcgc gcatgcggcg gcgagacgac tttcacgagc
      421 gccagttccc gcatgacgat cgctttttcg gtgatgtcat tcactttgat gacgtcgatc
      481 tgtttttgca gctgtttgac gagctgctca attttccgtt catcctccac atggacgatg
      541 aatgtcattt tcgacatgcc cggctgttcg gagtgtccga cggtgatgct ttcgatattg
      601 aattgccgtt tcataagcaa tcctgtcacc cggttcaata ctccgctttg attgatgact
      661 gtgaccgtaa tgactcttct cattcgctct tcaccccgat catttcttgt agtccttttc
      721 ctggcgcaac catcggatag acgttttcca gcattttcac ccggcaatcg ataagtaccg
      781 gttcgtccga caggagagcc tccctgaaga gttgctcggc atcctctttt ttcgtgatgc
      841 gaaccccttt caggtcatac gcttccgcga gcttgacgaa gtccggctgg actggaatca
      901 atgactgcga gtagcgttca tcgtagaagg tctcctgcca ttggcgcacc attccgagcg
      961 ctccgttgtt caagatgacg actttcaccg gaatcctcat ttcctgcagg agggatagct
     1021 cctgcgctgt catttggaat ccgccatcgc cgacgatagc gacgactctt tcatccggct
     1081 tcgcgagttg cgcgccgata gctgccggga atccgaagcc catcgttccg agaccgcccg
     1141 acgtgaccca atgatcggga ttgttcaatg aataatattg ggccgcccac atctgatgct
     1201 gcccgacgtc tgttgtgacg attgcatcgc cctcggtaat gcggtgaatc atctggatcg
     1261 cttcctgcgg caggatttca tgctcgtcgg tgttgtacca taatggcgcc tcttcctgta
     1321 gaccgttcaa atagtcgagc cattcttcag tttcaggcgc atggaattcc tgtttgagca
     1381 atgctttcaa cgcttccttc gaatccgcta cgatagggat ttccgtcggg acattctttc
     1441 cgatttccgc agggtcgata tcgatatgga tgacttttgc atgcggagcg aaactatcca
     1501 gattgcctgt cagcctgtcg tcgaatcggg cacctacgtt gatcaatacg tcgcactcgc
     1561 ttattgccat attcgccgta tacgttccgt gcatgcctgc catgccaagg aataattcat
     1621 ggtctccggc aatgctgcca agtccgagca atgtgttcgt aaccgggatc cgtttcgttt
     1681 ccaccaattc ttttaactct tccatcgcct gaccgtgcag cacgccagct ccagcaagga
     1741 tgagcggctt gtgtgcgaat cggatcgctt ccgccgcctt ctgtatttgc aagtaattcg
     1801 gagtggtcgt cggttggtag cccggcaaat tcacttcggc atttcgttct tcctccgtga
     1861 cgaaaatttc cgtagcgacg tttttgggga tgtcgacgac aacaggtcca cgtcttcctg
     1921 ttgaagcgat atggaatgct tctttgatga tccttggaag atccgcaacg tcctgcacct
     1981 ggtagttatg ctttgtgatt ggctgtgtta tgccgatcac atccgcctcc tggaatgcat
     2041 ccgtcccaat gacggtgctt gccacctgtc ccgtgaaaac aacgagcggc aatgaatcca
     2101 tcatcgcatc cgtaattcca gtcaccagat ttgtcgcacc tggacccgat gttgcaatga
     2161 caacacccgg tttgccggat actcgcgcat agccctccgc cgcgtggatg gctccctgtt
     2221 catgacgggc aagcacatgg gggatcggat ccttgtataa tgcgtcgtaa atcggcagga
     2281 cggcacctcc agggtaaccg aaaatcactt cgacaccttg atccttcaac ccttgaatca
     2341 agactgcaga accatccatc ggttgattat tctgtttttc gactgtagat tccggtgcca
     2401 tttccatgac tgcttgcttc gtttgaactt ccgcactcat atacgaactc ctccttactt
     2461 ttttctcatt gattgtttta tataaaagaa aaagcctttt caccccacac aaagaaacct
     2521 tggttcttcg tataagggat gaaaaagctt ttcatggtac cacccttgtt cgcagcattt
     2581 gccgcctcgt gaatgaacct acaaaaggtt cattcgctga taacgagcct gtttatcaca
     2641 agcccgggat tgcctactca gttccctttt cagcagtcca ctccggggag atatcgcgga
     2701 tagttgcatt gccggcttcc agcacgaccg gctctctgtg aatgcaagga tctatcccct
     2761 tttgccccat catcgattta ttatatcttc atgacgccgc ctgtacttgc ggacgtcact
     2821 agttttgaat atctggccaa atagcctttt ttgattttcg gttcaaatgg ctgcagtttt
     2881 gttctgcgct ccgccaattc ttcatca
//
LOCUS       DASULI010000235         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_196458, whole
            genome shotgun sequence.
ACCESSION   DASULI010000235 DASULI010000000
VERSION     DASULI010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196458"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFX34_05155"
     CDS             complement(<1..308)
                     /locus_tag="VFX34_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX5564324.1"
                     /translation="MRHKVPGSVLDEYIAYIRLEKNYSSRTVLEYSNDIQEFLAFLSQ
                     EGIADLNNVEYSEARLYITTLYDKGLARKTISRKISSIRSFYKFGNARFGINDNAFRL
                     "
     gene            complement(609..1928)
                     /gene="trmFO"
                     /locus_tag="VFX34_05160"
     CDS             complement(609..1928)
                     /gene="trmFO"
                     /locus_tag="VFX34_05160"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEX5564325.1"
                     /translation="MTPIVNVIGAGLAGSEAAWQIASRGVQVRLYEMRPVKQTPAHHT
                     DKFAELVCSNSLRANSLTNAVGIIKEEMRRLDSLIINAADACAVPAGGALAVDRHEFA
                     GNVTDRIRNHPLIEVINEEVTDLDEMMEGITVIATGPLTSPALADRIRELTGEEYLYF
                     YDAAAPIVEKDSIDMDKVYLKSRYDKGEAAYLNCPMNEEEFKRFHEALINAEVAPLKE
                     FEKEVYFEACMPVEVMAQRGEKTLLFGPMKPVGLEDPKTGKRPKAVVQLRQDDAAGTL
                     YNIVGFQTHMKWGAQKEVLQLIPGLENVEIVRYGVMHRNTFINSPRVLNSTYQLRENG
                     NIFFAGQMTGVEGYVESAGSGLIAGINAAKTALGKEPIEFPEETALGSMARYITEADP
                     QNFQPMNINFGLFPDLGERIKSKVERAEKHAERAFMALEQFKPRVQA"
     gene            complement(1953..>3935)
                     /gene="topA"
                     /locus_tag="VFX34_05165"
     CDS             complement(1953..>3935)
                     /gene="topA"
                     /locus_tag="VFX34_05165"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5564326.1"
                     /translation="GHLRDLPRSQMGVDTENNYEPKYITIRGKGPILQELKKDAKKAK
                     KIFLAADPDREGEAIAWHLAHQLGVDINSDCRVVFNEITKDAIKESFKNPRPIDMDRV
                     DAQQARRILDRLVGYNISPILWKKVKKGLSAGRVQSVALRLIIDRENEIKAFIPEEYW
                     SITGKFSKDGKTFEASFYGDATKKMKLENKKQVDEILSKMSGDEFEIVNVVKKERKRN
                     PAPPFTTSSLQQEAARKLNFRARKTMMLAQQLYEGINTGKEGIVGLITYMRTDSTRIS
                     DSAKGEAVSFIEKMYGEEFVATSKAKAKESANTQDAHEAVRPTSVMRPPEAMKAFLSR
                     DQHRLYKLIWERFVASQMAPAVLDTVTADLMNGDVKFRATGSEVKFPGFMKVYIEGEH
                     DQEEEKDRILPPLDKGERVKGSDIDPKQHFTQPPPRYSEARLVKTLEELGIGRPSTYA
                     PTLDTIQKRGYVTLDAKRFVPTELGEIVHQAVMQYFPDIINIEFTAEMEKSLDDVEDG
                     EVRWVQVIDEFYKEFEKHVKVADEEMEKIEIKDEPAGEDCEKCGSPMVFKMGRYGKFM
                     ACSNFPDCRNTKAIIKPIGVTCPSCKEGQVVERKSKTKRIFYGCDRYPECEYVSWDKP
                     IARPCPKCQSTLVEKRLKKGVQIQCTECDYKEDAQE"
BASE COUNT          885 a         1011 c          823 g         1216 t
ORIGIN      
        1 aataaacgga atgcattatc atttatcccg aatcgagcat tgccaaactt ataaaaagag
       61 cggatagaag aaatctttct cgaaattgtc ttccttgcca agcctttgtc atacagagtt
      121 gttatataca atcttgcttc ggaatattcg acattattca gatcagcaat tccttcttgg
      181 gataaaaagg caaggaactc ttgaatatca ttgctgtatt ccaaaactgt cctggatgaa
      241 tagttctttt caaggcgtat ataagctatg tattcgtcaa gaacagatcc gggaactttg
      301 tgtctcaagt gcttcacccc catatgaatc actctattaa ttatagcaaa attggaacat
      361 gtttgaatag tggttatttt ttcttttttt caggaccgct tctctattgt taatcgaata
      421 ggaggactaa ttcggaaatt caaaagtcat ccacgattca ctataacgta aagtatattg
      481 ttccggcaac ataatcgtct taggttgtct gacatctatc tttttttggt cacattttgt
      541 tcaattttca tgaacataca cagtaaaaag ctgcacgatg cggatagtcc gtcatgtgca
      601 gcaacacttt acgcttgtac tctgggcttg aattgctcta atgccatgaa tgcgcgttcg
      661 gcatgttttt cggcgcgctc gacttttgac ttgattcgct cgcctaaatc agggaataag
      721 ccgaagttga tattcatagg ctggaagttt tgagggtcag cctcggtaat gtatctggcc
      781 atgcttccta acgcagtctc ttcggggaat tcgattggct ctttgcccaa agccgtcttc
      841 gcagcattga ttccggcaat caagcctgaa cctgccgatt cgacatatcc ttcgacgcca
      901 gtcatttgac cggcaaagaa gatatttcca ttctcacgaa gttggtatgt tgaattaagg
      961 acccgcggag aattgatgaa cgtattccgg tgcatgaccc catatcttac gatttcaaca
     1021 ttttcaagac ccggaatgag ttggagcacc tctttttgcg ctccccactt catatgggtt
     1081 tggaaaccga caatgttata cagagtgccc gcagcatcat cctgtcgcag ttgtacgacc
     1141 gctttcggcc gtttgcccgt tttcgggtct tccagtccga cgggtttcat cgggccgaat
     1201 agaagggtct tctcgccgcg ttgggccata acttcgacag gcatgcacgc ttcgaaatac
     1261 acttcttttt cgaattcctt caatggcgct acttccgcat tgatcaatgc ttcgtggaac
     1321 cgtttgaatt cctcctcatt catcgggcag ttcaaataag cggcttcgcc tttatcgtat
     1381 cgcgatttca aataaacttt atccatatcg atggagtctt tctccacgat cggagcggct
     1441 gcatcgtaga aatagagata ttcctcgcct gtcagttccc ggatgcgatc agccaatgcc
     1501 ggtgaagtca atggcccagt tgcaatgaca gtaatccctt ccatcatttc gtccaggtcc
     1561 gtcacttctt cattgatgac ttcgattaac ggatgattcc tgattctgtc tgttacattt
     1621 ccggcaaatt catggcggtc gaccgcaagc gctcctcccg ccggaacagc gcatgcatcc
     1681 gcagcgttga tgataaggga gtcgagcctg cgcatctctt ccttgataat gcctaccgca
     1741 tttgtcaggc tgttcgctct caaagaatta ctgcagacca gctccgcaaa cttatccgta
     1801 tgatgtgcgg gcgtctgttt cactggtctc atttcataaa gccggacttg aactccacgg
     1861 gaagcgatct gccaagcggc ctcgctcccg gcaagtccag caccgatgac atttacgatt
     1921 ggtgtcattc agccttcctc ttttccatcc tcttattctt gtgcatcctc tttatagtca
     1981 cattccgtac attgaatctg cacacctttt ttcaatcgtt tttcgactaa tgtactttga
     2041 catttaggac acggtctggc aatcggctta tcccaagata cgtattcaca ctccggatat
     2101 ctgtcacagc catagaaaat cctttttgtc ttgctcttcc tctcgacgac ctgcccttct
     2161 ttacatgaag gacaagtgac cccgatcggt ttgatgatcg cttttgtatt tctgcagtcg
     2221 gggaaattcg agcaggccat gaattttcca tatcggccca ttttgaatac catcggggaa
     2281 ccgcatttct cgcaatcctc gccggcgggc tcgtccttga tttcgatttt ttccatctct
     2341 tcatcggcaa ctttgacatg cttctcgaat tctttgtaaa attcgtcaat gacttgcacc
     2401 catcggactt ccccatcttc aacatcatca aggctcttct ccatttctgc agtgaactcg
     2461 atgtttatga tatcagggaa gtactgcatc acagcttggt ggacgatttc cccaagctct
     2521 gtcggaacga accgcttagc atccaatgtg acatagcccc gtttttggat cgtgtcgagt
     2581 gtaggtgcat aagtagacgg acgaccgatt ccaagttcct caagcgtctt gacaagacgt
     2641 gcttccgaat accttggcgg tggctgcgtg aaatgctgct tcgggtcgat gtcgcttcct
     2701 ttgacgcgct ctcctttatc gagcggcggc agaatgcggt ccttctcctc ttcctgatcg
     2761 tgttcccctt cgatatacac tttcataaag cccggaaatt tcacttcaga gccggttgcc
     2821 ctgaatttga cgtcgccgtt catcagatcc gctgtgactg tgtcaagaac ggcaggtgcc
     2881 atctgacttg ctacgaaccg ttcccatatc aatttataga gtctgtgctg atcgcgcgat
     2941 aaaaacgctt tcatcgcttc aggcggcctc atgaccgatg tcggccgtac agcttcatgc
     3001 gcatcttgtg tgttggccga ttccttcgcc ttcgctttgg aagtggcgac aaattcctcg
     3061 ccgtacattt tttcgatgaa ggaaaccgct tctccctttg cactatcgga aatcctcgtc
     3121 gaatccgttc tcatatacgt tatgagaccg acgatgcctt cttttccggt attgatccct
     3181 tcatacagct gttgggcaag catcattgtt ttccgcgccc tgaaattcaa tttgcgtgca
     3241 gcctcctgtt gcaaggagga agtcgtaaat ggaggggcag gattcctttt cctctccttt
     3301 ttgacgacgt tgacgatttc aaattcatcg cctgacattt tggacaagat ttcatcgacc
     3361 tgttttttat tctccagctt catcttcttc gtcgcatcac cgtagaatga agcttcgaat
     3421 gttttcccgt ctttgctgaa tttcccagtg atgctccaat actcttcagg gatgaatgcc
     3481 ttgatttcat tctcccggtc gatgatcaat cgcaatgcga ctgattgcac acgtccggct
     3541 gacaatccct ttttcacttt cttccaaagg atcggactaa tattatagcc tacgagtcta
     3601 tccaaaatcc ttcttgcctg ttgggcatcc acgcgatcca tatcgatcgg ccttgggttt
     3661 ttgaaagatt ccttgatggc atcctttgtg atttcgttga agacgacgcg gcaatccgaa
     3721 ttgatatcaa cgcccaattg gtgtgccaaa tgccatgcaa tcgcttcccc ttctctgtcg
     3781 gggtcagctg cgagaaagat tttcttcgct tttttagcat cttttttcaa ctcttgcaat
     3841 atcgggcctt ttccgcgtat cgtgatgtat ttcggttcat aattgttttc ggtatccaca
     3901 cccatctgac tgcgcggcaa atcccgcaag tgccc
//
LOCUS       DASULI010000236         2638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1919791,
            whole genome shotgun sequence.
ACCESSION   DASULI010000236 DASULI010000000
VERSION     DASULI010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919791"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..843)
                     /gene="tpiA"
                     /locus_tag="VFX34_05170"
     CDS             complement(82..843)
                     /gene="tpiA"
                     /locus_tag="VFX34_05170"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5564327.1"
                     /translation="MRKPIIAGNWKMYKTLEEAKQFAEEVKGLAPDPEKMDAVICSPA
                     PYLAQLVQVLDGYPVAVGAQNMHYEDEGAFTGEVSPVQLKDLSVQYVIIGHSERREYF
                     NETDEAVNKKAHAALKYELTPIICVGETLEEREAGNTEKKVSTQVRAALEGINDSQVI
                     QSVIAYEPIWAIGTGKTATAEDANEVCGAIRKTVAELYNDETAQAVRIQYGGSVKPEN
                     IDELLSMEHIDGALVGGASLKTDSFVKLLEAGSNA"
     gene            complement(856..2043)
                     /locus_tag="VFX34_05175"
     CDS             complement(856..2043)
                     /locus_tag="VFX34_05175"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5564328.1"
                     /translation="MQQKQSINDVQLDGKRVFCRVDFNVPMESGNITDDTRIRAAIPT
                     IEKLIDKGAKVILASHLGRPKGEVNDDMRLTAVGERLSELLGKPVNKLDESIGAEVEA
                     AVNAMGNGDIILLENVRFHKGEEKNDPELAKSFANLADIYVNDAFGAAHRAHASTEGI
                     TKHIPAVSGLLMEKELSVLGSALSSPERPFTAIIGGAKVKDKIGVIEYLLEKVDNLII
                     GGGLSYTFVKANGHEIGKSLLEEDKIDLAKSFIETAKQKGVNLYLPEDAIVAKEFSAD
                     SETKVVEIDAIPSDWMGLDIGPKTVQTYAEVIKNSKTVIWNGPMGVFEMEPFAAGTKG
                     VADAMAETVAYTIIGGGDSAAAVEKFEVADKMDHISTGGGASLEFMEGKELPGVSALN
                     EKE"
     gene            complement(2154..>2638)
                     /locus_tag="VFX34_05180"
     CDS             complement(2154..>2638)
                     /locus_tag="VFX34_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5564329.1"
                     /translation="TTIHSYTNDQRILDLPHSDYRRARAAGQSMIPTSTGAASAVAKV
                     LPELKGKLDGMAVRVPTPNVSLVDFVAELNTNVTIEDVNAALKEASEGELKGFLSYND
                     LPLVSADYNGDKSSSTVDGLSTMVLEDNMVKVLSWYDNETGYSTRCIDLALHMYAKGL
                     "
BASE COUNT          650 a          679 c          520 g          789 t
ORIGIN      
        1 ttcttcgcat gggcgatcgc atttccgaac gtttcctcgc ggagtgcgaa accatccaga
       61 ataatgaggg cgactggaga cttaggcatt tgaaccagcc tccagaagct tgacgaagga
      121 atctgtcttc aaactcgctc ctccgaccaa ggcaccatcg atatgctcca ttgaaagaag
      181 ctcatcgata ttttccggct taacgctgcc gccatattgg attcgtactg cttgggcagt
      241 ctcatcatta tatagttcag caacagtttt tcggattgct ccgcacactt catttgcatc
      301 ctcggcagtt gcagtttttc cagttccgat tgcccagatt ggctcataag caatgaccga
      361 ttgtatgact tggctatcat tgatgccctc aagcgctgct ctaacctgtg tcgatacttt
      421 cttttccgtg ttgcccgctt cgcgttcttc aagagtctct ccgacacaaa taatcggagt
      481 caattcgtat ttcagtgcag catgtgcttt cttattaacc gcttcatcgg tttcgttgaa
      541 atactctcta cgctcggaat gtccgataat cacgtattgc acacttaggt cttttaattg
      601 gacaggactt acttcacctg tgaaagcacc ttcatcctcg taatgcatgt tttgcgcacc
      661 gacagctacc ggatagccat ccaacacttg gacaagctga gctaaatacg gtgcaggtga
      721 acaaatgaca gcatccattt tttcaggatc cggcgcaagc cctttcactt cttcagcaaa
      781 ctgtttagct tcctcaagcg ttttatacat tttccagttg cctgcaataa ttggtttcct
      841 catgccgatt cctccttatt ccttttcatt caacgcactg acacctggca attctttccc
      901 ttccatgaat tccagggaag cccctccgcc agtggaaatg tgatccattt tgtcagccac
      961 ttcaaacttt tcaacagcag ctgcggaatc cccgcctccg ataatcgtgt aagcgacagt
     1021 ttcggccatc gcgtcagcca caccttttgt tccggcagca aatggttcca tttcaaagac
     1081 acccatcgga ccgttccaaa tgactgtttt tgagtttttg atgacttctg catatgtttg
     1141 tacagttttc ggaccgatat caagccccat ccagtctgaa gggatggcat cgatctccac
     1201 taccttcgtt tcgctgtcag cggagaattc ttttgctacg atggcatcct ccggcaaata
     1261 gaggttaacc cctttttgct tcgctgtttc aataaacgat ttagctaagt caatcttatc
     1321 ctcttctaat agagatttac cgatttcgtg gccatttgct tttacaaacg tataagaaag
     1381 accgccaccg atgatcagat tatctacctt ctcaagcaag tattcaatga caccaatttt
     1441 atccttcact ttcgctccac cgataattgc agtaaatgga cgttcaggtg atgacaaagc
     1501 actacctaaa acagacagtt ctttttccat cagtagaccg gaaactgctg gtatatgttt
     1561 ggtaatccct tctgtcgatg cgtgtgcacg gtgagcagca ccgaaagcat cgtttacgta
     1621 gatgtctgct aaattagcaa agctcttcgc cagttcagga tcgttctttt cttcgccttt
     1681 atggaatcgg acattctcca ggaggatgat gtccccatta cccatagcat tgaccgctgc
     1741 ttcgacttcc gccccgatgg actcgtccag tttgttcacc ggcttgccta agagttcgga
     1801 taatcgttct cctacagccg tcagccgcat gtcgtcgttc acttcacctt ttggacgccc
     1861 aaggtggctc gccaagatga ctttcgcgcc tttgtctatc aatttttcga tggtcggaat
     1921 cgcagcccgg attctcgtat cgtctgttat atttccggac tccatcggta cgttaaagtc
     1981 cactcggcaa aatacacgtt tgccatccaa ttgaacatca ttaattgact gtttttgctg
     2041 catgaagaaa aacctcctac atgtatgtaa atgacaaaaa aacaaggaac gggtaacccc
     2101 cgttccttta ccctatttca ttatagaggt tccattgtct gtttggctat tgattataat
     2161 ccctttgcgt acatatgaag agcaaggtcg atacagcgag tagaatagcc tgtttcgtta
     2221 tcgtaccatg aaaggacttt aaccatattg tcttccaata ccattgtcga taatccatca
     2281 acagtcgaag aagatttgtc gccattataa tcagcagaaa ctaacggaag atcgttataa
     2341 ctcaagaaac ctttaagttc accttcggaa gcttccttca gcgctgcatt cacgtcttca
     2401 atcgttacat tcgtattcaa ttctgcaaca aagtctacca atgaaacgtt aggtgtcggt
     2461 acacgaacag ccataccatc caacttacct ttcaactcag gaagaacttt cgcaactgcc
     2521 gatgctgctc ctgtagatgt aggaatcatg gattgtcctg cagcacgtgc acggcggtag
     2581 tcactgtgcg gtaagtcaag gattcgttgg tcatttgtat aggagtgaat agttgtca
//
LOCUS       DASULI010000237         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1322547,
            whole genome shotgun sequence.
ACCESSION   DASULI010000237 DASULI010000000
VERSION     DASULI010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322547"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VFX34_05185"
     CDS             <1..1017
                     /locus_tag="VFX34_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5564330.1"
                     /translation="IIYAIMALGVYLSFRVLDFPDLTVDGSFVSGAAVAATMILFGYH
                     PAIATAEALIVGFLAGCVTGLLHTKGKINPLLSGILMMIALYSINLRIMGLTSENSIG
                     RPNIPLLNAETLFSQFQAFLAPLGVDNMMNQFFSMLGVRYLPSTWGTVFIMAIIVLLI
                     KIVVDWFLKTEVGLAIRATGDNKKMIRSLSANTDTLVIIGLGFSNALVAFSGALFAQY
                     AKYADIGMGIGMIIIGLASVIIGEAIFGTKTIVRTTFAVIAGAIIYRIVLALALRIDF
                     LDTGDMKLITAVIVICALVLPQYFEKRREKARKARRHEERSAERNFSLNKEGNAIVEA
                     RTDK"
     gene            992..1789
                     /locus_tag="VFX34_05190"
     CDS             992..1789
                     /locus_tag="VFX34_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564331.1"
                     /translation="MLKLEQINKVFNEATPDEKIALDQINLELKSGDFVTVIGSNGAG
                     KSTMLNMISGALAPDFGKIEIAGKDVTKLPEYVRSRMIGRVFQDPMAGTAPTMTIEEN
                     LAMAYSRNKKRMLRRGVDKKRREFFRSSLETLHLNLENRLNAKVGMLSGGERQALSLL
                     MATFTQPSILLLDEHTAALDPSRAELITNLTKELVEKDQLTTLMITHNMQQALDLGNR
                     LIMMDKGQIIIEVGAEEKKGLTIDKLMAEFQRIRGEQLTSDKALLSV"
     gene            1973..2230
                     /locus_tag="VFX34_05195"
     CDS             1973..2230
                     /locus_tag="VFX34_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564332.1"
                     /translation="MSNMHEVEMGSLYERSWNGKLDGNICELLSTLPPNSTVAEILVD
                     GLPFAVTAFVNLNTDTKLAYFTSVIGTTLIVDCDSISQVIF"
     gene            2277..2498
                     /locus_tag="VFX34_05200"
     CDS             2277..2498
                     /locus_tag="VFX34_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564333.1"
                     /translation="MRKGDESDMARTAICDLMETFPTNFALTSIVVDGHQQTVTSLTT
                     IDRDRSLAIFNYGGSTVFAECKKISLIEI"
     gene            complement(2665..>3089)
                     /locus_tag="VFX34_05205"
     CDS             complement(2665..>3089)
                     /locus_tag="VFX34_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX5564334.1"
                     /translation="DKEEVERGEVSLHKDVVEEEQSFDVPVMREEVYVERRPVNEYES
                     DVDEFKMMQDDETIRVPITEERLEVKKKPYVSEEIVVGKRQVEDTEMVNETVKREEAR
                     FEQSGNVDVQDEFIDEPTSGSNWEDEPTSRQKWDNERF"
BASE COUNT          835 a          695 c          736 g          823 t
ORIGIN      
        1 atcatctatg caatcatggc actcggagtg tatctatcat tccgagtgct tgatttcccg
       61 gatctgacgg tagatggaag ttttgtatcg ggagctgcag tggctgctac aatgatacta
      121 ttcggctatc atccagccat cgcaacggcc gaagccctca tcgtcggttt cttggccggt
      181 tgtgtaaccg gccttctgca tacaaaagga aagatcaatc ctctcctttc cgggatctta
      241 atgatgattg ctttgtattc cattaatttg cgcattatgg gccttacatc cgaaaactcg
      301 attggccgcc ctaacattcc tttattaaat gccgagacgt tatttagtca atttcaagcg
      361 tttttagctc cactaggcgt tgataatatg atgaatcagt ttttcagtat gctcggtgtc
      421 cgctatttac cttctacgtg gggaactgta ttcatcatgg cgatcatcgt tctactcatt
      481 aaaattgttg tggattggtt cttgaaaact gaagtgggac ttgcgattcg ggcaacggga
      541 gacaataaaa aaatgattag gagtctatcg gcgaacacgg atacactcgt catcatcggc
      601 cttggtttct cgaatgcgct cgtcgccttt tcaggagcgt tattcgcgca atacgcgaag
      661 tatgccgata tcggcatggg aattggaatg atcatcatcg gtttggcctc cgtcattatc
      721 ggtgaagcga tctttggaac aaaaacaatt gtgcggacga cttttgcggt tattgcaggc
      781 gcgatcattt atagaattgt tctggcactt gcgctcagga ttgatttcct cgatacaggg
      841 gatatgaagt tgattacagc ggtgattgtc atctgcgcgc tggtattacc gcagtatttt
      901 gaaaagaggc gtgaaaaagc gagaaaagcg aggcgtcatg aggaacgttc ggccgaacgc
      961 aatttttctc tcaacaagga ggggaatgca attgttgaag ctcgaacaga taaataaagt
     1021 attcaatgaa gcgacgcctg atgaaaagat tgcattggat caaatcaacc tcgagttgaa
     1081 atctggtgat tttgtgaccg tgatcggaag caatggagcc gggaaatcga cgatgttaaa
     1141 tatgatttca ggagctcttg cccccgactt tggcaagatt gaaattgcag gtaaagatgt
     1201 tacaaagctg ccggagtatg tacgttcccg aatgattgga agagtattcc aggacccgat
     1261 ggcaggcacc gcccccacga tgacaatcga ggagaatctg gcaatggcgt attccaggaa
     1321 taaaaagcgt atgttgcgta gaggtgtcga taaaaaacgc cgggaatttt tccgcagctc
     1381 gctcgaaacc ttgcatttga atttggaaaa caggctgaat gcaaaagtgg gcatgttatc
     1441 cggcggagag cgtcaagcgc tgtctttgct aatggcgaca tttacccaac cgtccatttt
     1501 actgctggat gagcatacag ctgcacttga tccttcccgt gcagaattga ttacaaattt
     1561 aacaaaagaa cttgttgaaa aagatcaatt aacaacgctg atgatcacac ataatatgca
     1621 acaggcactg gatctcggga acaggctcat tatgatggat aaaggccaga tcatcataga
     1681 agtgggagcc gaagagaaga aggggctgac aatcgataaa ctgatggctg aattccagcg
     1741 aattcgcggt gaacagttga cgagcgacaa agcgttgcta tccgtttgag tagattggga
     1801 agaactaaac gatcacctaa ctggtgagcg tttagttttt tattgttgag aaaaaagtat
     1861 ggacaaccaa gcggacgtcc tatatgggaa gaaatttcaa caatacagta taaggacccc
     1921 ttgaatgcaa taggggtcag gggttttccg atcgaaaggg ggtgaccaag ccatgagtaa
     1981 catgcatgag gtggaaatgg gcagcttgta tgagcgtagc tggaacggca aactcgacgg
     2041 gaatatatgt gaactgctgt cgacgttgcc gccgaactcc acggtggcgg aaatactcgt
     2101 tgacgggctg ccgttcgcag tgactgcgtt cgtcaacctg aacacggata cgaaacttgc
     2161 atatttcaca tcagtcatcg ggactaccct cattgtagat tgcgatagca tctcccaagt
     2221 gattttctag gattttcttg gatgaatcat ggacatgcta cccaagatgc acatctatga
     2281 ggaaaggaga tgaatcggac atggcaagaa cagcgatttg tgatctaatg gaaacttttc
     2341 cgacgaactt cgcgttgacg agtattgtgg tagatggaca ccagcaaacg gtcacctcat
     2401 tgacgacaat cgatcgagac agatcccttg ctatcttcaa ttacggagga tcgaccgttt
     2461 ttgcagaatg caaaaagatt tccctgatcg aaatctgatt tgtcgacatc gggcagagaa
     2521 ggcattcact tcaccaaaaa atccataggt cctcccaatc atcatccaac ataaaaagtg
     2581 tctcacccca agacaggcac tcgacataga aaaagcccgc taagcatttt tcagctcagc
     2641 gggccccatt tatttttctg ttacttaaaa ccgctcgtta tcccatttct ggcgacttgt
     2701 tggctcatct tcccaattac ttccgctcgt aggctcgtcg atgaattcat cctgcacatc
     2761 gacattcccc gattgctcaa agcgggcttc ctcacgtttg acggtttcat tgaccatttc
     2821 agtgtcctcc acttgtcgtt taccgacgac gatttcctcc gatacatacg gcttcttctt
     2881 cacttcaagc cgctcctcgg taatcggcac gcggatcgtt tcatcgtcct gcatcatttt
     2941 gaactcatcg acatccgatt cgtactcatt gacaggacgg cgctcgacgt aaacttcctc
     3001 gcgcatgact gggacgtcaa aggactgctc ctcctcaacg acatctttat gaagggaaac
     3061 ttcgcccctt tcgacctctt ccttatcca
//
LOCUS       DASULI010000238         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1550285,
            whole genome shotgun sequence.
ACCESSION   DASULI010000238 DASULI010000000
VERSION     DASULI010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550285"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFX34_05210"
     CDS             complement(<1..343)
                     /locus_tag="VFX34_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEAT domain-containing protein"
                     /protein_id="HEX5564335.1"
                     /translation="MRRRVAPWLGAVLLLWAIASMPVGAESLSDGVYAVEYELLQGDS
                     DSVSIANDYFVKPALLKVDGDQRVLQLTMNHSEWVRELQEYNGEDFTDAKIVAEDAEA
                     DTRVIELNVVGE"
     gene            858..1946
                     /locus_tag="VFX34_05215"
     CDS             858..1946
                     /locus_tag="VFX34_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HEX5564336.1"
                     /translation="MNKVGSISILHVILLSMTVIGLKNHVTIIPSLLVHAGRDAWISV
                     FIAAVAMVPWLFLLVFIHRSSKQVSLTDWLQTKIGKGPTAIFRYVTAIFLFLMAIFTM
                     GETIQWIKATFLPDSPFILMILIYTILCIFLAATNLQTIVMVNVLVLFWVVILGFFVA
                     FTNLQVKDYLLLRPFFEHGFKPVVSSLVYPASGFVELLLFLFIQQNVKGKFRWYHFAV
                     MLFILHSLTMGPLVGAITEFGPDEAAKQRYPAYEEWGLVSIGRFIQHLDFLSIYQWLT
                     GAFVRVAFILYVVTDLLQIKGDRIQIWRILAPAFFFMSLALFLLNDQVFLKIKGDYLL
                     IATFFFMLAVTLFLTIMASIKKGTAKEV"
     gene            1973..3412
                     /locus_tag="VFX34_05220"
     CDS             1973..3412
                     /locus_tag="VFX34_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX5564337.1"
                     /translation="MDITSLRQTFEKSADIHFQTYTFNGHTVIFITCEAMVDHQLLHE
                     IIVPRVGHFVSHLENRPIEKTIEAELHIPDLKAGSDLEDIIPLVYTGHVLLFFQDSGL
                     LYTSNIAQKPNRSPEETRLEVPVKGPRDNFIEDVSVNIALLRKRLPTNSLCVEKFELG
                     TRSKTTVALLYFDDIANKEILHGIRKQLQEVDIDIVISPTLLMELVNKSSSLFPRIDY
                     TGRPDNAVQSLVRGRFLIFVDGAAYGVVLPVNLFLLVKTGEDNETPPIYSSMERLMRI
                     FGLSVGLTLPAFWLALTTYHQNQLPLQLLATVVQANTGLPLPSSIEMLLMLLMFELFR
                     EAGLRLPTALGGTIGVVGGLIIGDAAIRAGITSPAMIVVIAISTISTFTLVNQSLVNA
                     VSILRVGFILLTALLGLFGLLMSVFLTIVFLSNIRTFGVPYMNISTDLSFKTIMESFF
                     RLPQNFYTKRPKMMKPTDPTRMKKVDKDE"
     gene            3405..>3645
                     /locus_tag="VFX34_05225"
     CDS             3405..>3645
                     /locus_tag="VFX34_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HEX5564338.1"
                     /translation="MNKRMIGFILLLLAALSLSGCWDNNEPERMLYVNGIGIDYKNGQ
                     YELYAQIINFSNTAKSEQPTNIETTQAEVGHASGKS"
BASE COUNT          968 a          905 c          746 g         1026 t
ORIGIN      
        1 actcgccgac cacgttcagt tcgatgaccc gcgtatctgc ttccgcgtcc tccgcaacga
       61 ttttcgcgtc cgtgaaatct tcaccgttat attcctgcag ctcccggacc cattcgctat
      121 gattcatcgt caactgcaaa acccgctgat cgccatccac cttcaacaac gccggcttca
      181 cgaaataatc attcgcaatc gacaccgaat ccgaatcgcc ctgcagcagc tcatactcca
      241 ctgcatatac accgtcggac aacgattccg cccctacagg catagacgca atcgcccaca
      301 acagcaaaac agcaccaagc caaggcgcca cccgtctcct catgctaacc ctctcccact
      361 agtgagaact gttatcaatt tacaccccca tcataggaca agagcccccc atttgaaagc
      421 cgccccacaa ctattcacac attgttcaca gaaaaaagac cgaattgtgt ccacagcctt
      481 ttgtaggtgc ctgtgcttgg tgcatttatg acaattcaat ccaatcaata aaaatgcaat
      541 aggaagccag ccaaatgcaa atatggcggg cttcctatct tattaatatt caattgtagt
      601 gaatagactg aattgtcccg gtgcctgtgc aaggtgcaat tatgataatt cgtttataag
      661 tataaaaata caccaagatg tctcccaaac ttcgtttcaa caaacttccc acttctcaaa
      721 tatgcaattg tagttaataa acagaatagt ttgttgcaca ggcaccttca tacaaaggaa
      781 ccttccgaaa accgcgctta taatccttcc gtttcgtgtc tagactatgc aatatcatta
      841 gggtgggagt tttgggtatg aataaggttg ggtccatcag tattttgcat gtgattttat
      901 tgtcgatgac ggtcattgga ttgaaaaatc atgtgaccat tattccgtcg cttttggtac
      961 atgcggggcg tgatgcgtgg atatctgttt tcattgcagc agtggcgatg gttccgtggc
     1021 tttttcttct tgtattcatt catcgcagct caaaacaggt ttcgctcacc gattggctgc
     1081 aaacgaagat cggcaaaggc ccgaccgcta ttttccggta tgtcacagcc atttttctct
     1141 ttctgatggc catttttaca atgggcgaga cgatccagtg gattaaagcg acattcctgc
     1201 ccgattcccc ttttatcctc atgatcttga tttacacgat tctatgtatc tttcttgccg
     1261 ccacaaattt gcaaacgatt gtcatggtga atgtcctcgt cttattttgg gttgtcatcc
     1321 tcggcttttt tgtcgcattc accaacttgc aagtgaagga ttatttattg ctgagaccat
     1381 tctttgagca cgggttcaaa cccgtcgtat cttcattggt gtatccggct tcaggcttcg
     1441 ttgagcttct gttattcctg ttcatccagc aaaacgtgaa aggcaagttc cgctggtatc
     1501 attttgcggt catgcttttc atcctgcaca gtttgacgat ggggcctctt gtcggtgcaa
     1561 tcacggaatt cggccctgac gaagcggcaa agcaaaggta tccggcatac gaggaatggg
     1621 ggctcgtttc catcggacgt ttcatccagc atcttgactt cctgtccatt taccaatggc
     1681 tgacgggtgc gttcgtacga gtggcgttca tcctgtatgt cgtcacggat ttattgcaaa
     1741 tcaaaggcga ccgtatacag atttggcgaa tactagctcc tgcgttcttc ttcatgagct
     1801 tggcgctttt tctactaaat gaccaggttt tcctcaaaat aaaaggtgat tatctattaa
     1861 tcgcaacatt cttctttatg ctagcggtga cacttttcct aacaatcatg gcgtcaatta
     1921 aaaaaggaac tgcaaaagaa gtataaacct taactagaaa atggtgagtg ctatggacat
     1981 tacatcgtta agacaaactt tcgagaagtc tgcagatatt cattttcaaa cttacacatt
     2041 caatggccat acagtcatct ttattacatg tgaggctatg gtcgaccacc aactgttgca
     2101 tgaaatcatc gtgccgcgtg tcggccattt cgtcagccac ttggaaaata ggccaatcga
     2161 aaaaacgatt gaagcggaac tgcacatccc cgacttgaaa gcgggaagtg acttagaaga
     2221 tatcattccc ctcgtctaca cagggcatgt cctattattt tttcaggatt ccggacttct
     2281 ctacacaagc aatatcgccc aaaaaccaaa tcgcagtccg gaagaaacac gattggaagt
     2341 gcccgttaaa ggtcctcgtg acaatttcat cgaagacgtg tccgtcaata tcgccttgtt
     2401 aaggaaaaga cttccaacca actcattatg cgtggagaaa ttcgaattgg ggacgcgctc
     2461 caaaacgact gtcgcactat tatactttga tgatattgcg aataaagaaa tcctgcacgg
     2521 catacgtaaa cagctccagg aagtcgatat cgacatcgtt atcagcccta cacttctaat
     2581 ggaacttgta aataaaagct cctccctctt cccacggatc gattatacgg gacgcccgga
     2641 taatgccgtc cagtcgcttg tgagaggccg gttcctcatt ttcgttgacg gggctgcata
     2701 tggcgtcgtt ctgcccgtca acctattttt gcttgtaaag acgggcgagg ataatgaaac
     2761 acctcccatc tatagttcca tggagcgttt aatgcgcata ttcggattgt cagtcggttt
     2821 gacattgccc gcattttggc ttgcactgac aacctatcac cagaaccagc ttccattgca
     2881 attattggca acagtcgtac aagcaaatac agggcttccg ctcccttcat ccattgaaat
     2941 gctgcttatg ctgctcatgt ttgaactgtt ccgggaagcc ggattgcgcc tgccgacagc
     3001 gctaggaggc acaatcggcg ttgtcggcgg cttgatcatc ggagatgccg ccatccgggc
     3061 aggcattaca agccccgcga tgattgtcgt tatcgctatt tcaacaattt caacatttac
     3121 attggtcaac caatcactag tgaatgctgt cagtatatta cgggtcggct tcatcttgct
     3181 caccgctcta ttaggtttat tcggccttct catgtccgtt ttcctgacga ttgtcttctt
     3241 atcaaacata cggactttcg gcgttcctta tatgaacatt tcaactgact taagctttaa
     3301 aacaattatg gaatcattct tccggctccc ccagaatttt tatacaaaac ggcctaaaat
     3361 gatgaaaccg acagacccaa cacgtatgaa gaaggtggat aaggatgaat aaaagaatga
     3421 tcggttttat tctgcttctg ctagcggcgc tgtccttatc cggctgttgg gataataatg
     3481 aacctgaaag gatgttatac gtcaacggaa tcggcatcga ctataaaaac ggtcaatacg
     3541 agctgtatgc ccaaattatt aatttttcca atactgccaa atccgaacag ccaacaaata
     3601 tagaaacaac acaagcagag gtcggtcatg cttccggaaa atcat
//
LOCUS       DASULI010000239         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_202369, whole
            genome shotgun sequence.
ACCESSION   DASULI010000239 DASULI010000000
VERSION     DASULI010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202369"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFX34_05230"
     CDS             complement(<1..385)
                     /locus_tag="VFX34_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5564339.1"
                     /translation="MEKRKLWIVLIGVLLLIAVAGCGKKAATAAKENSLIYASESEFD
                     GLNPILEETNVDALLFRGLFRFDENNEPKADIASSFTMSDDKLTYTFKLQEDIRFHDG
                     EPLTAEDVIFTIESVLDDKNASFLKS"
     gene            complement(401..1210)
                     /locus_tag="VFX34_05235"
     CDS             complement(401..1210)
                     /locus_tag="VFX34_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX5564340.1"
                     /translation="MVSKVARRHHNGKLDMVFELRRGYTRMPHVFQQPPLKASRELYE
                     GTDPTATVYVMESSGGMVAGDRNDISIRLLPGSKARIIQQSALKVYRSHTGETCVQQI
                     DVEVEEEARLEWMPEVIIPFADSKFRMETTIQLAKGATLLWGEIIAPGREMRGEVFDF
                     SSLKSMMKIYVEGELIAFDSLRFVPEDLHLNGLGLLEEALYVGSIWLVSEKADEIDLR
                     AIQEALKVGDGLRAGVTRLAGNAVHCRFLGVNQWRLQQEMKRVFSELSALI"
     gene            complement(1200..1826)
                     /gene="ureG"
                     /locus_tag="VFX34_05240"
     CDS             complement(1200..1826)
                     /gene="ureG"
                     /locus_tag="VFX34_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX5564341.1"
                     /translation="MKPVRIGIGGPVGSGKTSLVDALTRAMHENYNVAVITNDIYTRE
                     DAQYLMKHGVLAEDRILGVETGGCPHTAIREDASMNFAAIDDLKERFNDLDIVFVESG
                     GDNLSATFSPELVDGYIYVIDVAEGQDIPRKGGPALTRSDLLLVNKTDLAPHVGVDLE
                     LYESDVKKMRGGRPYVLADVRTRKNVDQIVNWIKRNMLLEGAGTASGE"
     gene            complement(1845..2585)
                     /locus_tag="VFX34_05245"
     CDS             complement(1845..2585)
                     /locus_tag="VFX34_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HEX5564342.1"
                     /translation="MKQQNADSNNLSNTAAIPTNIQFLRLLQIHDSAFPIGTYTQSYG
                     METYIQEDAIRTKEELIAFCTTFLHQNLVYGDAILIQEAHKAARSRDTDRLLHLETLC
                     GAIKLAKESRDASVNLGRQFIRTVSPLIENDFFDEWKNRISANEIKGHYAILYGIYCA
                     VNDVDSHYAVLSYLFSSVNGLVTNAVRAVPFGQNTGVQAVHQLTEEIVAAAGIVNGLT
                     EADISNNALGIELASMKHEYLYSRLFIS"
     gene            complement(2527..>2833)
                     /locus_tag="VFX34_05250"
     CDS             complement(2527..>2833)
                     /locus_tag="VFX34_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX5564343.1"
                     /translation="LRIDQEEPLKYGDLLFEDDNRAIVIRTKMEPVIVIRPKDMVEMG
                     KAAFELGNRHTPALIEKDEIVVRADHTLAPLLDEVGVDYETTERRFKQPFKYRGHSH"
BASE COUNT          668 a          776 c          627 g          762 t
ORIGIN      
        1 cggatttcag gaacgaggca ttcttgtcat ctaatacact ttcaattgta aagatgacat
       61 cctccgcagt cagcggttca ccatcgtgga accggatgtc ttcctgtaac ttgaatgtat
      121 acgtcaactt gtcatcggac atggtgaatg acgatgcgat atcggctttc ggttcgttgt
      181 tttcatcaaa acggaacagg ccgcggaaaa gaagcgcgtc cacattcgtt tcctcgagaa
      241 tcggattcaa cccgtcaaac tcggactccg aagcgtagat gaggctattt tctttcgcgg
      301 cggttgccgc tttttttcca catcccgcta cggcgatgag cagaagtacc ccgattaaaa
      361 ctatccataa ttttcgtttt tccatagttt cccccgaatt ttaaatgagc gccgacagct
      421 cggaaaagac ccgtttcatt tcctgctgca gtctccattg attgacgccg agaaaacggc
      481 aatgcaccgc atttcccgca agtcttgtta caccagccct taatccgtca ccgactttta
      541 acgcttcctg gattgcccgt agatcgattt catcagcctt ttccgaaaca agccaaatgg
      601 agccgacata caatgcctct tcaagtaaac caagtccatt aagatgcaaa tcttcaggaa
      661 cgaaacggag gctgtcgaat gcgattagct ccccttcgac atagatcttc atcattgatt
      721 tcaatgaaga gaaatcgaat acttctccac gcatctcccg gccgggcgcg atgatttcgc
      781 cccatagcaa tgtagcaccc ttcgcaagtt ggattgtcgt ttccatccgg aacttggaat
      841 ccgcgaacgg gatgatcact tccggcatcc attcgagacg cgcctcttct tcaacttcca
      901 cgtcaatctg ttgcacgcac gtttcacctg tatgcgaccg gtatactttc aatgccgatt
      961 gctggatgat ccgcgctttg cttcccggca ataggcggat tgaaatatca ttgcggtcgc
     1021 ccgccaccat gccgcccgat gattccatta catagaccgt tgctgtaggg tccgtgccct
     1081 catatagctc acggctcgcc ttcaaaggag gctgctggaa tacatgcggc atccgcgtat
     1141 agccccttct cagttcaaac accatatcca gcttaccatt atggtgcctg cgggccactt
     1201 tactcaccac ttgctgtccc tgcgccttct agcaacatgt tccgtttgat ccaattgacg
     1261 atctggtcga catttttccg tgtccggaca tccgctaaca cgtaaggacg accgcctctc
     1321 attttcttca cgtcgctttc gtacagttca aggtcgacgc cgacatgggg tgcaaggtcc
     1381 gttttattga ccaataacag atcggatctc gttaacgccg gaccgccttt tctcggaata
     1441 tcctgtcctt ccgcaacgtc gatcacgtag atatatccat caaccagctc agggctgaat
     1501 gtcgctgaca agttgtcccc gccgctctcg acgaaaacga tgtcaagatc gttgaagcgt
     1561 tctttcaaat catcgattgc tgcgaagttc atggaagcat cttcacggat tgccgtatgc
     1621 gggcatccgc cagtctccac accgaggatc ctgtcttcag ccaacacacc gtgcttcatg
     1681 aggtattgag catcttcgcg tgtataaata tcattcgtaa ttactgcaac gttatagttt
     1741 tcatgcattg cacgggtcaa tgcgtcgacg agcgatgtct tgcccgatcc gacgggaccc
     1801 ccgatgccga tacgtactgg tttcatatat agaaacgcct cctgtcatga aatgaataat
     1861 cttgaatata aatattcgtg cttcatcgat gcaagctcga tgccaagcgc gttattgcta
     1921 atgtccgcct ccgtcaaccc gttgacaatc ccggcagctg caacgatctc ttccgtcaac
     1981 tgatgaactg cctgcacgcc ggtattttgt ccgaatggca ccgcacgtac ggcgtttgtg
     2041 acgagcccgt tcaccgatga gaataaatac gaaaggaccg cataatgcga atccacatcg
     2101 ttgacggcac aataaatacc gtaaaggatt gcataatggc ccttgatttc attcgcactg
     2161 atccggtttt tccactcatc gaaaaaatca ttctcgatga gcggtgaaac cgtacggatg
     2221 aactgcctgc ccaagttgac gctcgcatcc ctggattcct tcgcaagctt gatcgctccg
     2281 caaagggttt ccaaatgcag aagacggtcg gtatccctac tccttgcagc cttatgcgcc
     2341 tcctgaatga ggatcgcatc gccatatacg agattttgat gaaggaatgt cgtacaaaat
     2401 gcaatcagtt cttccttcgt ccggattgca tcttcctgga tatacgtctc catcccgtat
     2461 gactgtgtat acgtgccgat cgggaatgcc gagtcgtgga tttgaagcag tcttagaaat
     2521 tgtatattag tgggaatggc cgcggtattt gaaaggttgt ttgaatctgc gttctgttgt
     2581 ttcatagtcc acacccacct catccaataa tggcgccaac gtgtgatctg cgcggacgac
     2641 aatttcatct ttttcaatta aagcaggcgt atggcggttg ccaagctcaa aggctgcttt
     2701 gcccatctcg accatatcct tcgggcggat gacgatgacc ggttccattt tcgttcggat
     2761 cacgatggcg cgattgtcgt cttcgaacag aagatcgccg tatttcaacg gctcttcctg
     2821 gtcaatgcgc aat
//
LOCUS       DASULI010000240         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_80103, whole
            genome shotgun sequence.
ACCESSION   DASULI010000240 DASULI010000000
VERSION     DASULI010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80103"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFX34_05255"
     CDS             complement(<1..649)
                     /locus_tag="VFX34_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX5564344.1"
                     /translation="MNAKRTSQITAVTGILLAAAIGLHLAGLHDWRQWLLISSTVLAG
                     IPIAIKAFKALKMKAFSIELLVTIAVIGALFIGEYIESAAVTFLFLFGAYLEVRTLEK
                     TRSSLKALVDMAPLEATVVRDGETMTISVDNVEVGDRVIIRSGEKVATDGRIITGNAT
                     LNEAAITGESVPASKTVDDRVFSGTIVDNGFIEVIAERVGDDTTFARIIELVEEAQ"
     gene            complement(664..885)
                     /locus_tag="VFX34_05260"
     CDS             complement(664..885)
                     /locus_tag="VFX34_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX5564345.1"
                     /translation="MKKIVLNLEPLTCPSCIKKIEGALNRMDGVVEAKVLFNSGKVRA
                     QFDESKIAAEAIANTVASLGYPVLSQKVS"
     gene            1166..1846
                     /locus_tag="VFX34_05265"
     CDS             1166..1846
                     /locus_tag="VFX34_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5564346.1"
                     /translation="MELVYKGKTKDVYKLEDGNVLLKFKDDVTGEDGVFDPGANTVGL
                     TIEGAGQSGLRMTKFFFEKLAEKGIPTHYVAADIDAATMTVKPATVFGKGLEVICRYR
                     AVGSFLRRYGAYCEEGQPLDAFVEVTIKDDDRNDPPITKDALAQLGILTNEEYGILEP
                     MTKQISGVVKEELSAKGLELYDIKLEFGRDEEGNIMLIDEISGGNMRVYKDGVYIAPL
                     DLEKLMLA"
     gene            1992..2549
                     /locus_tag="VFX34_05270"
     CDS             1992..2549
                     /locus_tag="VFX34_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX5564347.1"
                     /translation="MKHKLVVLLLISVILVVAGCGKAKQKVPVSGEQMEAIVSPLLNT
                     EGEEIGEVTLVQEAKGVLISVQAEDLPPGTHGIHIHETGVCTPPDFTSAGAHFNPTGK
                     EHGFDNPKGFHLGDLPNIEVPEDGKVAVQLPTAEITLKKGQENSILDKDGSAIVIHEK
                     ADDYKTDPAGDSGARIACAAITGKK"
     gene            complement(2593..>3276)
                     /locus_tag="VFX34_05275"
     CDS             complement(2593..>3276)
                     /locus_tag="VFX34_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5564348.1"
                     /translation="LPDEALLFLSDVLPTAYWSVEHAGVKHGDTVAVLGCGPVGLMAQ
                     KFAWMKGAKRVIAIDHVPYRLRHAVKMNKVEAFNFDDYDNMGAHIKEITRGGVDIVID
                     CVGMDGKKNIAEKVGQKLKMQGGTLSAVEIGYQAVRKFGTIHLTGVYGSLYNMFPLGN
                     MFERNITLKMGQAPVIHYMPTLFDKITKGEFDPTEIITHVVPIEEASAAYHHFYEHED
                     DCIKVILKP"
BASE COUNT          864 a          758 c          824 g          830 t
ORIGIN      
        1 cctgcgcttc ttccacaagt tcgatgatgc gcgcaaatgt cgtatcatca ccaacgcgct
       61 ccgcaatcac ttcgatgaac ccattatcaa cgattgtgcc gctgaaaaca cggtcatcaa
      121 ccgtcttcga agcggggacg gattcgccag ttatcgctgc ttcattcaac gttgcattcc
      181 ctgtgatgat ccgaccatcg gtagcgactt tttcccctga tcggatgatg acccgatctc
      241 ctacttcaac attgtcgacc gaaatcgtca tcgtttcacc gtcgcggacg actgtcgctt
      301 caagcggggc catatcgacg agcgccttta aggaagaacg tgttttttcg agcgtgcgta
      361 cttcgaggta tgcgccgaat aaaaacagga acgtcacggc tgctgactcg atgtattcac
      421 cgatgaacaa tgcgcctata acggcgatcg ttacgagcaa ttcaatacta aaagctttca
      481 tctttaaagc tttaaatgct tttatggcaa ttgggatgcc ggccaatact gtggaagaga
      541 tgagcaacca ttgcctccaa tcatgaagtc ctgcgagatg gagcccaatt gccgccgcaa
      601 gcagaatgcc tgtcacggcg gtaatttggg aagttcgttt ggcattcatt gtgcttcacc
      661 ctttcatgag actttttgtg agaggacagg atatcctagg ctagcaactg tgttcgcgat
      721 ggcttctgcg gcgattttgc tttcatcaaa ttgtgctcgg acttttccgg agttgaacag
      781 tactttcgct tctactacac cgtccatccg gtttaacgcg ccttcgattt tcttgatgca
      841 agatgggcaa gtcaacggct ccaagttcaa tacgattttt ttcaatttcc tctacctcca
      901 tggttttgtt ttgttcttct tgactacatc ataaggcgtt ttccgggatg tagccttgac
      961 ctccgtcaag ttttcacttt ttataggacg aaaagtgcct tatactataa agtgaacaaa
     1021 gaactttgct atataagata gactgagcgc agcgagggag attgaagagt gccttaattt
     1081 ctgcgaaaac gcagaactac ggcaaatcga actctgcgtg gttccgattc actacatttt
     1141 cgaatagaga ggaaacgtgg gcagcatgga attggtttat aaaggaaaga cgaaagatgt
     1201 ttataaattg gaagatggca acgtgttatt gaagtttaaa gatgatgtga ccggggaaga
     1261 cggtgtgttt gatccgggtg cgaatacagt aggattgacg attgaaggtg cggggcaatc
     1321 gggcttgcgc atgacgaagt tcttctttga gaaattagct gaaaaaggga ttccaacaca
     1381 ttatgtcgct gcggatatcg atgcggcgac gatgaccgtg aagccggcga ccgtattcgg
     1441 gaaagggctt gaagtcattt gccgatatcg tgcggtcgga agcttcttgc gtcgatacgg
     1501 ggcgtattgc gaagaaggac aaccgttgga cgcatttgtt gaagtgacaa tcaaggacga
     1561 tgaccgcaat gatccgccaa tcacgaaaga cgcactcgct cagctcggca ttttgacgaa
     1621 cgaggaatat ggcatcctcg agccgatgac aaagcagatt tccggcgtcg tgaaggaaga
     1681 gctctccgcg aaggggcttg aactttacga catcaagctg gaattcggtc gtgacgaaga
     1741 aggcaacatc atgctgatcg atgaaatttc cggcggcaat atgcgtgttt ataaagacgg
     1801 agtttatatt gctccgctag atcttgaaaa attgatgcta gcgtaaatga aaaccgctgt
     1861 tcattccgaa aaatggaatg tgcagcggtt tttatgtttc tttctcgaca aatgaacggg
     1921 aaaagtttcc atacataatt cccgtctagt tggacacact tggcaagagt aaatgttgag
     1981 gaggaatcat agtgaaacac aaactggtag tcctactgct gattagcgtc attctcgtag
     2041 tggcgggatg tgggaaagct aaacaaaagg tgcctgtcag cggggaacag atggaagcga
     2101 tcgtgtctcc tttattgaac acggaagggg aagagattgg tgaagtgaca ttggtccaag
     2161 aggcgaaagg agtgctcatc agtgtgcagg cggaagactt gccgcctggg acgcatggta
     2221 ttcacatcca tgaaactggc gtatgtacac cgccggattt cacttccgct ggagcgcact
     2281 tcaatccgac cgggaaagaa catggctttg ataatccgaa aggtttccat ttaggcgatt
     2341 tgccgaatat cgaagttccg gaagacggga aagtggcggt tcagctgcca acagccgaaa
     2401 tcacgctgaa gaaaggccag gaaaactcaa tccttgataa ggatggaagc gccatcgtca
     2461 ttcacgaaaa ggcggatgac tataagacag atcctgcagg ggattccggc gcacgtatcg
     2521 cctgcgcggc aattacaggg aagaaataaa aaaaacggtc catgcaacga ttgcgtggac
     2581 cgattcttgt gtttacggct tcaatatcac tttaatgcaa tcatcttcat gttcatagaa
     2641 atgatgatac gcggctgagg cttcctcgat cggaacgaca tgcgtgataa tttccgtcgg
     2701 atcgaattcg ccttttgtaa tcttatcaaa caatgtcggc atgtaatgga tgactggagc
     2761 ttgccccatc ttcagcgtaa tattccgctc aaacatattc ccgagcggaa acatgttata
     2821 gagggatccg tagacgcccg taagatggat cgtcccgaac ttacggacgg cctgatagcc
     2881 gatttcaacg gcgcttaacg taccgccttg catcttcaac ttctgtccga ccttctccgc
     2941 gatgttcttt tttccatcca tcccgacaca atcgatgacg atatcaacgc cgccgcgggt
     3001 gatttcttta atatgcgcgc ccatattgtc atagtcatcg aaattgaaag cttccacttt
     3061 gttcatcttc acggcgtggc gcagccgata aggaacatgg tcgatcgcaa tgacgcgttt
     3121 cgctcctttc atccaagcga atttctgcgc catcagcccg acaggaccgc aaccaagcac
     3181 agcaaccgta tctccatgct tcacgccagc atgttcaacg ctccaatagg cggtcggcag
     3241 cacatcggac aggaaaagca gcgcctcgtc gggaag
//
LOCUS       DASULI010000241         7946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1796634,
            whole genome shotgun sequence.
ACCESSION   DASULI010000241 DASULI010000000
VERSION     DASULI010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796634"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFX34_05280"
     CDS             complement(<1..476)
                     /locus_tag="VFX34_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsfA family transcriptional regulator"
                     /protein_id="HEX5564349.1"
                     /translation="MVKVRQDAWLEENDILLAETVLRHVREGSTQLSAFEEVGDALNR
                     TAAACGFRWNAVVRRDYEKELAEAKKERKQAMRVLGADFKRRSQQLYSPLQGVDQDEK
                     TAVPLSALSLDTVIAYLIRLHHTGGGDSESLRWKQTAKMANEKVETLQKEIDKLQQ"
     gene            659..1954
                     /locus_tag="VFX34_05285"
     CDS             659..1954
                     /locus_tag="VFX34_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5564350.1"
                     /translation="MDYKNEKLFEEKVFKDPVHRYIHVRDKVIWDVIGTREFQRLRRI
                     RQLGTTYLVFHGAEHSRFQHSLGVYEIVRRIIDDGFSGRAEWNDEERLLTLCAALLHD
                     LGHGPFSHAFEKVFTLDHEKFTQAILSGDTEVNAVLRRVSPDFPQQVADVIGKTHPDK
                     LVVSMISSQIDADRMDYLQRDAYYTGVSYGHFDMERILRVMRPAEEQVVIKFSGMHAV
                     EDYIMSRYQMYWQVYFHPVSRSAEVILMKILHRATHLYNSGYPFKYDPVHFKSFFEKT
                     FDLEEYISLDEGVLMTYFQWWRKEEDPILADLCDRFLNRRLFQYAEFNPATEYRKIGE
                     LEKLYKEAGLDPEYYLVTDSSSDLPYDFYRPGEENERVPIYLQMPNGELSELSRSSEI
                     VDAISGKRRTDHKMYFPEDVLESKGNPLYKKILQMLRGE"
     gene            1956..2465
                     /locus_tag="VFX34_05290"
     CDS             1956..2465
                     /locus_tag="VFX34_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwgA family protein"
                     /protein_id="HEX5564351.1"
                     /translation="MLQEHARIVQFIALAGEARGRKKLQKMIYIAKKMDFPFAEKYEL
                     HMYGPYSEELTLRVEELCEMGFLKESCTDKGSYVQYSYNVTEEGGRFLETADAPHEKL
                     ADCIDRLNGRSSRFLELVSTLLYFDYLPKDEQIAKVHIVKNKLNFTEDEMQDAFEFIA
                     ELQACSGVA"
     gene            complement(2712..2900)
                     /locus_tag="VFX34_05295"
     CDS             complement(2712..2900)
                     /locus_tag="VFX34_05295"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HEX5564352.1"
                     /translation="MPYVTVKMLEGRTEEQKRNLVEKVTDAVAETTGAPVENVVVFIE
                     EMPKNHYAVAGKRLSDKE"
     gene            2969..3499
                     /locus_tag="VFX34_05300"
     CDS             2969..3499
                     /locus_tag="VFX34_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HEX5564353.1"
                     /translation="MTNEQKPKRKMGFTIIKDDPTDGHGGFGIGSLSLENVSPVIIDI
                     EEGEARIEIGAMHARSDTERGVKFTTNREDSEGGKPYWLVWVTIDFKEDGPYYAGVTA
                     CEMVVNREKRRGYKVLADHVNKMDKSMKRHIMVDHMDDKSKQILADFLSTHNKEMWER
                     SEPKLRIDLGAESPEL"
     gene            complement(3848..5905)
                     /locus_tag="VFX34_05305"
     CDS             complement(3848..5905)
                     /locus_tag="VFX34_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX5564354.1"
                     /translation="MRRVDYIKKKKRHHFVRRIALLFVTAVTAFFTMFLALRLYAQIT
                     GAPSLSVPKASVVLDKNGRQIGDKFSAERRYWVGLDEISPFLLDAVIATEDRNFYKHN
                     GFDYKRIAGAVLKDIKTMRKAEGASSITMQYARNLFLTHEKSWNRKIKESIYAYRLET
                     FYDKDVILEGYLNTIYFGHGMYGVEAASRFYFAKSAAELTLEESALITAIAKGPSVFD
                     PLNNPERSRERQLLILSLMEDQGFITEVQRQRAADVAVTLRTEDWKDMKRVAPYFLDE
                     VWREAERILSKKGRYPAEGGWTIRTTLDPQHQQSAEEYIKKWMPDSGLQVGFVSMETE
                     TGAITSLVGGMDYNESPFNRVTQAKRQPGSAMKPILYAAALEDGYNPLTFLSTERTIF
                     TYDEGRSTYEPQNVNGKFAGHPISLAQAIAISDNIYAVKTLEDIGYKKFNKMANRLGL
                     DVEFQESPAVALGTSTVSLLEMTTAYDRIASGGLEVEPTTIISITDAEGKTVYEQPKK
                     STKRVITEQDAFVLTHMLTGMFDPAFNDYSVATGLSMRSKQKRPYAAKSGTTISDQYL
                     IGYTPSLTAGIWTGYDVGQQLTNLEDKAATKKIWIDFMETVHAGKTPEPFIPPKGVSS
                     AIVDVETGGIAVNECPKQRLMYMKEKDLPQKLCTDKNLREQRNGEDHDSKTFELFPFS
                     FFE"
     gene            6078..6503
                     /locus_tag="VFX34_05310"
     CDS             6078..6503
                     /locus_tag="VFX34_05310"
                     /inference="COORDINATES: protein motif:HMM:NF020711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEX5564355.1"
                     /translation="MESTKESRNVDVRLRSTIRHVGQTADKHELTAAGTLIEKAGKTY
                     LQFTEDLNGQQVRTTVKLEEGDALIMRNGAVQMRLPFSLGEMRPGTYGNGPATFDLLV
                     KTTKLEVGADKFTAHYELHGEGGLLGKHELTITYTEGQK"
     gene            6500..>7946
                     /gene="argS"
                     /locus_tag="VFX34_05315"
     CDS             6500..>7946
                     /gene="argS"
                     /locus_tag="VFX34_05315"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5564356.1"
                     /translation="MNAVEKIQQEIKDAFREAVLASELADESAMPDIILETPKNKENG
                     DYATNIAMQLTKIAKKPPRAIAEAILEKLDTSSTSIKSLEIAGPGFINIRLKSDYLGE
                     VVKTVLAQGADYGRSTYGENLKVQVEFVSANPTGDLHLGHARGASIGDALCNILDFAG
                     FDVSREYYINDAGNQVNNLALSVEARYFQALGLEKKMPEDGYHGQDIIDIGEKLAEEF
                     GDKYVNVGEKERQEFFRKYGLEFELAKLKKDLEDFRVPFDNWFSETSLYTDGKIGIAL
                     DKLRANGHVFEEDGATWFRSTTFGDDKDRVLIKNDGTYTYLTPDIAYHEDKLRRGFDK
                     LINIWGADHHGYIPRMKAAIEALGYDRDTLEVEVAQMVQLYKDGEKFKMSKRTGKAVT
                     LRELVEEVGLDAVRYFFAMRSADSQMDFDLDLAISQSNENPVYYSQYAHARISSILRQ
                     ADEANLAASTDHVDLLQTEKELSLLKKIGDFP"
BASE COUNT         1974 a         1980 c         2062 g         1930 t
ORIGIN      
        1 tcctgctgca gtttgtcgat ctctttttgg agagtctcga ctttttcgtt tgccatttta
       61 gcagtctgct tccatctgag tgattcggag tcgcctccgc ctgtatgatg taaacggatt
      121 aagtaggcaa ttactgtatc aagcgataag gcggataaag ggacagccgt cttttcatcc
      181 tgatcgaccc cttgcaaggg gctatataat tgctggctgc gccgtttgaa gtccgcgccc
      241 aatacgcgca ttgcctgctt ccgttctttt ttcgcttctg ccagttcttt ttcgtaatcg
      301 cgccggacga cagcattcca tctgaaaccg cacgcggccg cggtcctgtt gagcgcatct
      361 ccgacctctt cgaacgcgct tagctgtgta ctgccttccc ggacgtgcct aagcaccgtc
      421 tcggctaata aaatatcatt ttcctcaagc caagcatcct gtctaacttt caccatctcc
      481 ttgcctcctg ccatgttcat agtctctttc ttacggtcag tgtgtacagt gtttaagcct
      541 tttattcatc caaacttgaa cgcattgcaa aataattgca ttctttttga tgaaaacctt
      601 ccttttctct tgcccggttt ttgatatact taagaaaatg cgtcgatgga ggtgcgcgat
      661 ggattataaa aatgaaaagc ttttcgaaga gaaagtcttc aaggatccgg tccaccggta
      721 tatccatgtg cgcgacaaag tcatatggga cgtcatcggg acgcgggaat tccagcggct
      781 gcgcaggatc cggcagctcg ggacaacgta tctcgttttc cacggggcgg aacatagccg
      841 cttccaacac tcgctcggcg tctatgaaat tgtgaggcgg atcattgacg acggcttcag
      901 cggaagagcg gaatggaatg atgaggaacg gctgttgacg ctgtgcgcgg ctttgctgca
      961 tgacctcggg cacggtccgt tttcgcatgc gttcgaaaag gtgttcacgc tcgatcatga
     1021 gaaatttacg caggcgattc tgtcagggga cacggaagtg aatgcagtgc tgcgccgcgt
     1081 ctcgcccgat tttccgcagc aggtggcgga tgtcatcggg aagacgcatc cggacaagct
     1141 tgtcgtcagc atgatttcga gccagatcga tgctgaccgg atggattatt tgcagcgtga
     1201 cgcctattac actggcgttt cgtacggcca tttcgatatg gagcggattt tgcgcgtcat
     1261 gcggccggcc gaagagcaag tagtcatcaa gtttagcggc atgcacgcag tggaagatta
     1321 cataatgagc cgctaccaaa tgtactggca agtctacttc caccccgttt cgcggagcgc
     1381 ggaagtcatt ctcatgaaaa tcctgcaccg tgcaacccat ctatataaca gcggctatcc
     1441 gttcaaatat gatcctgtcc atttcaagtc gtttttcgag aagacattcg acctggagga
     1501 atacatatcg ttggatgagg gcgtgctcat gacgtatttc caatggtggc gcaaagagga
     1561 ggatccaatc ttggcggacc tatgcgaccg tttcctcaac cggaggctgt tccaatatgc
     1621 cgagttcaac ccagcgacgg aataccggaa aatcggcgag ctcgaaaaac tgtacaagga
     1681 agcgggcctt gatccggaat actatctcgt cacggattcc tcttccgatc tcccgtatga
     1741 tttctaccgg cccggcgaag agaatgagcg cgtgccgatc tatttgcaaa tgcccaacgg
     1801 tgaactgagt gagctgtccc ggtcatcgga aatcgtcgac gccatttcgg gcaagcggcg
     1861 taccgaccat aaaatgtatt ttcccgaaga tgtattggaa tcaaagggaa atccgttata
     1921 taagaagatt ttacaaatgt tgagaggtga gtaggttgct gcaggagcat gctagaattg
     1981 tgcaattcat tgccctggcg ggagaagcgc gcgggcggaa gaaattgcag aagatgatct
     2041 atattgcgaa gaagatggat ttccctttcg ctgaaaagta cgagctgcat atgtatggcc
     2101 cgtattcgga agaactgacg ctccgtgtcg aggagctttg cgaaatgggc tttttgaagg
     2161 agagctgcac ggacaagggt tcgtacgtcc aatatagcta caacgtgacg gaggaaggcg
     2221 gccgattcct tgaaacggcg gatgcgcctc acgaaaagtt ggccgactgt attgatcggc
     2281 tgaatggcag gagctcgagg ttcttggagc tggtgtcgac attgctctat ttcgattatt
     2341 tgccgaagga cgagcaaatc gcgaaagtgc atatcgtgaa gaataaactg aatttcaccg
     2401 aagacgaaat gcaagatgct tttgaattta ttgcggagct gcaggcgtgt tcgggtgttg
     2461 cataataaga aaatcccctt ttgtatgagg ggattttttt tgtttgaatc gcgtcggagg
     2521 agttggccgc gctcaaagat ggttcgtccg cgctcaagaa ctccgctccc acgctcaaga
     2581 attcggattc gcgctcaaga actccgcccc cgcgctcaag aattcggatt cacgctcaag
     2641 atttctgccc ccgcgcccaa agccatcccc cagcaaacaa aaaacgatgc gagattctcg
     2701 catcgctcca attattcctt atcactcagg cgcttgcccg cgaccgcgta atggtttttc
     2761 ggcatttcct cgatgaagac gacgacgttc tcaacaggcg cgcccgttgt ttcagcgact
     2821 gcatccgtca ctttttcaac taaattgcgt ttctgctctt ccgtacgccc ttcgagcatt
     2881 ttcaccgtta catatggcat actaattccc cctttcggct aaatttatgt atagtgtata
     2941 taatagcaga atgggagggg ttataggaat gacgaatgag cagaaaccga aacggaaaat
     3001 gggattcacc atcatcaaag atgatccgac cgacggacac ggaggattcg gaatcgggtc
     3061 attgtcactc gaaaacgtct cgccggtcat catcgatatc gaagaagggg aggcgcgcat
     3121 cgaaatcggg gcgatgcatg ctcgaagcga tacggagcgc ggcgtgaaat tcacgacgaa
     3181 ccgcgaagat tcggaaggcg gaaaaccgta ctggctcgtc tgggtgacga tcgatttcaa
     3241 ggaggacggc ccatattatg caggcgtcac cgcatgcgaa atggtcgtga accgcgaaaa
     3301 gcgcagaggc tataaagttc ttgcggatca tgtgaataaa atggataaat caatgaagcg
     3361 gcatataatg gtcgatcata tggacgataa atcgaaacag atccttgcag atttcctgtc
     3421 gacccacaac aaagaaatgt gggagcgcag cgaaccgaaa ttgcgcatcg atcttggagc
     3481 agaatcacct gaattatgaa caaactgtga aatgtgatga cttcctcggc ttccgaggtt
     3541 gaatgtctgc gaaaaaggaa gtacactgaa cttacagctt gcgattgtaa gctaggacaa
     3601 ccggaataat gcgcctcctg tcaattgccg ggtctacata acccaagcat tgatccgaca
     3661 tgcgataggc cgcatttgac ggtaaaaaag ctttgcctga ctgatttaat tcagaaaggc
     3721 aaagcttttt tgattttcgt gtagacttct cattcttcat cgaagagttc tcgttaaagc
     3781 ccctggactc atcgtcaaag acttttcatt aaagccccga gacttctcgt tcccagcacc
     3841 cgcttgttca ttcaaaaaac gaaaacggaa aaagttcgaa tgttttcgag tcatggtctt
     3901 caccgttccg ctgttcacgc aagtttttat cggtgcacag cttttgcggc agatcctttt
     3961 ccttcatata cataaggcgc tgcttcggac attcgttgac cgcgatgccg cctgtttcga
     4021 cgtcgacaat ggcgctgctg acgcctttcg gtggaatgaa cggctccggt gttttgccgg
     4081 cgtggaccgt ttccatgaag tcgatccata ttttcttcgt ggcagccttg tcttccaggt
     4141 tcgtcaattg ctggccgaca tcataacctg tccaaatccc cgccgtcaat gaaggggtat
     4201 agccgatcaa gtattggtcg gaaatggtcg tgcctgattt tgccgcatac ggccgttttt
     4261 gcttcgaccg catcgataat ccggtagcga ctgaataatc attgaaagct gggtcgaaca
     4321 tgccagtcag catatgggtg agcacgaatg catcctgctc ggtgatgacc cgttttgtgc
     4381 tcttcttcgg ctgctcgtac accgttttcc cttccgcgtc ggtaatggaa atgattgtcg
     4441 tcggttccac ctcgagccca cccgaagcaa tccggtcata cgcagtcgtc atttccagca
     4501 aggaaacggt cgaagtaccg agtgcgacag cgggcgattc ctggaactcg acatcgagac
     4561 cgagccggtt cgccatttta ttgaactttt tatacccgat atcctccaat gtcttcacgg
     4621 cgtaaatatt atccgaaatg gcgatcgcct gcgcgaggga aatcggatgg cccgcaaact
     4681 tgccgttgac attttgcggc tcgtacgtcg agcggccttc gtcgtatgtg aaaatggtcc
     4741 gttccgtcga caggaacgtg agcgggttat agccatcttc aagtgccgcg gcgtaaagaa
     4801 tcggcttcat cgcggacccc ggctgccgct tcgcctgtgt cacccggttg aaagggctct
     4861 cattgtaatc catcccgccg acaagtgaag tgatcgctcc ggtttccgtc tccatcgata
     4921 cgaaaccgac ttgcagcccg ctgtccggca tccatttctt gatatattcc tccgcacttt
     4981 gctgatgctg cggatcgagc gtcgtccgga tcgtccagcc gccttcagcc ggatagcgtc
     5041 ctttcttgct tagaatacgc tccgcttcgc gccatacttc atccaaaaag taaggggcga
     5101 cccgcttcat atccttccaa tcctcggtcc tcagcgtcac tgcgacatcc gccgcccgct
     5161 gccgctgaac ttcggtgatg aatccctgat cctccataag cgaaaggatc aataattgcc
     5221 gctcgcggga acgctcggga ttgttcaacg ggtcgaaaac cgatggacct ttcgcaatcg
     5281 ccgtaatgag tgcggattcc tccaatgtca gctccgccgc cgacttcgcg aaataaaacc
     5341 tgcttgccgc ttcgacgcca tacatcccat gtccgaaata gattgtattc aaatagcctt
     5401 ccaaaatgac gtccttatcg taaaaggttt ccaaccggta cgcgtagatg gactctttta
     5461 ttttccgatt ccacgatttt tcatgtgtca gaaataaatt ccgggcatac tgcatagtga
     5521 tggagctcgc gccttccgct ttccgcatcg tcttgatgtc tttcaagacg gccccggcga
     5581 tccgtttgta atcaaacccg ttatgcttat agaaattgcg gtcctccgtt gcgattaccg
     5641 cgtcgagcag gaacggcgaa atttcatcaa gaccgaccca ataccggcgt tccgcggaaa
     5701 acttatcgcc gatctgccgc ccgttcttgt cgaggaccac actcgctttc ggtacgctca
     5761 acgaaggggc ccccgtaatc tgcgcataga ggcgcaaggc taggaacatt gtgaaaaacg
     5821 ccgtcactgc cgtcacaaaa agcagggcaa tccgtcgaac gaagtgatgt cttttctttt
     5881 tctttatata atcgacccgt ctcatacaat cccttctccc accgttcttt gctgtcagta
     5941 tggaaccggc gctcaaaagt taaacggcaa atcggtaaaa acatagattt gattccgaca
     6001 attcgcgatg tattggaata gtgcggggaa aaacgatata atggattgat attttgaacg
     6061 tcaagggcgg tgcaacagtg gaatcaacta aggagagccg aaatgtcgat gtccggctcc
     6121 gatcaacgat ccggcatgtc ggccaaactg cggacaagca tgagctgaca gctgcgggga
     6181 cattgatcga aaaagccgga aagacgtact tgcaattcac ggaagacttg aacggtcagc
     6241 aagttcggac gacggttaaa ttggaagaag gggacgcatt gatcatgcgg aacggggcgg
     6301 tgcagatgcg actgccattt tcgcttggtg aaatgcggcc ggggacgtac ggaaacggtc
     6361 ccgccacttt cgatcttctc gtgaagacga cgaagctgga agtcggggcg gacaaattca
     6421 cggcgcatta cgaactgcat ggtgaaggcg gattgcttgg caaacacgaa cttacgatta
     6481 catatacgga gggacaaaaa tgaacgcagt tgaaaagatc caacaggaaa tcaaagacgc
     6541 attccgcgaa gcggtgctcg cttcggaact tgcggatgaa tcggcaatgc cggatatcat
     6601 cctcgaaacg ccgaaaaaca aagagaacgg cgactatgcg acgaatatcg caatgcagct
     6661 tacgaaaatc gcgaaaaagc cgccgcgtgc aatcgcggaa gccattctcg agaaattgga
     6721 cacatcgtcg acttccatta aatcgttgga aatcgcggga ccgggcttca tcaacatcag
     6781 attgaaaagc gattatttag gcgaagtcgt caaaacggtg cttgcccaag gagccgatta
     6841 cgggcgttct acgtatgggg agaatttaaa ggtccaagtc gaattcgtct ctgcgaatcc
     6901 gacgggcgac ttgcatctgg gacacgcacg aggggcgtcg atcggtgatg cattgtgcaa
     6961 tatcctcgat tttgccggct tcgatgtatc aagggaatat tacatcaatg acgcgggcaa
     7021 tcaagtgaat aatctagccc tttcagttga ggcgcgatat ttccaagcat tgggtcttga
     7081 aaagaaaatg ccggaggacg gctaccatgg acaggacatc atcgatatcg gcgaaaagct
     7141 tgcggaggag ttcggcgaca agtatgtcaa cgtcggtgaa aaggaacgcc aggagttttt
     7201 ccggaagtac ggccttgagt tcgaattggc gaagctgaag aaggacttgg aagatttccg
     7261 tgtaccgttc gacaactggt tttcggaaac atcgctctac acggacggaa agatcggcat
     7321 cgcgctcgat aaattacgtg caaacggcca tgtattcgag gaagatggcg cgacttggtt
     7381 ccgctcgacg acgttcgggg acgataaaga ccgcgtgctt atcaagaacg atggaacata
     7441 cacgtatttg acgccggaca tcgcgtatca tgaggacaaa ttgcgccgcg gtttcgacaa
     7501 gctcatcaac atttggggag ccgaccacca cggctatatt ccgcggatga aagcggcgat
     7561 cgaggcgctc ggctacgacc gtgacacgct tgaagtggaa gttgcgcaaa tggtgcagct
     7621 gtataaggat ggcgagaagt tcaagatgag caagcggaca ggtaaagcgg tcacgctgcg
     7681 cgaacttgtc gaagaagtcg ggttggacgc agtccgttac ttcttcgcga tgcgctcggc
     7741 tgattcgcaa atggacttcg acctcgattt ggcgatttcg cagtcgaatg aaaacccggt
     7801 ctattattcg caatacgcgc atgcacgtat ttcatcgatc ctccgtcaag cggatgaagc
     7861 aaaccttgct gcctcgacgg atcatgtgga tttattgcag acggagaagg aattgtcact
     7921 gctgaagaaa atcggtgact tcccgc
//
LOCUS       DASULI010000242         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_663643, whole
            genome shotgun sequence.
ACCESSION   DASULI010000242 DASULI010000000
VERSION     DASULI010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663643"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFX34_05320"
     CDS             <1..288
                     /locus_tag="VFX34_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX5564357.1"
                     /translation="TEALFRMKIHPGRATERISEKRKRELFREIVDVLQESIDAGGSS
                     ISDYRNINGEAGTMQTRLKMYGKKVCGTCGKETKSMKIAGRTSVYCPQCQR"
     gene            305..901
                     /gene="coaE"
                     /locus_tag="VFX34_05325"
     CDS             305..901
                     /gene="coaE"
                     /locus_tag="VFX34_05325"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407739.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5564358.1"
                     /translation="MIIGLTGSIATGKSTVSRMLKQKGYPIVDADEISRLVVAPGSLV
                     LDQIATAFGTDVLLPDGSLNREKLGSLIFNDVAMREKLNGIIHPAVRAEMLRQKEEWL
                     EKGANTIIMDIPLLFESKLQSYVDKIIVVSATPVIQRKRLIARNGYSAEEADARINSQ
                     LPISEKEKGADAVINNNGTLDETEEQLNGILLKWDAIQ"
     gene            1029..2051
                     /locus_tag="VFX34_05330"
     CDS             1029..2051
                     /locus_tag="VFX34_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX5564359.1"
                     /translation="MTTSIAINGFGRIGRMVFRQIITKDDVDVVAVNASYPAETLAHL
                     IKYDTNHGVFDGKVVVEENALVVNGKRVQLIAERDPSKLPWKELGVDVVIEATGVFNE
                     RSKAALHLDAGAKKVILTAPGKNEDVTVVLGVNDDKLDIEKHDVISNASCTTNCLAPV
                     AKVLNDTFGIENGLMTTVHAYTNDQKNLDNPHKDLRRARACAQSIIPTSTGAAKALSL
                     VLPELEGKIHGMALRVPTPNVSLVDLVVDVKKDVTVEEVNEAFIKAAEGPLSGILRFT
                     TEPLVSIDFNTTTESATIDGLSTIVMGDRKVKVLAWYDNEWGYSARVVELAIKVGNEL
                     KSKVNS"
BASE COUNT          676 a          457 c          570 g          571 t
ORIGIN      
        1 acggaagcgc tctttcgaat gaagatccac cccggcaggg cgacggaacg gatcagtgaa
       61 aaaaggaagc gggaactgtt ccgggaaatt gtcgatgttc tgcaagagag catcgatgcg
      121 ggaggcagtt ccatctcgga ttatcggaat ataaacgggg aggcgggaac gatgcagacc
      181 cgtctgaaga tgtatgggaa gaaagtgtgc ggcacatgcg gcaaagagac aaaaagcatg
      241 aaaatcgccg gaaggacttc tgtctattgc ccgcaatgcc aaaggtgagg aaaggatgat
      301 ttcaatgatc atcggattga caggaagcat cgcgaccggt aaaagcactg tgtcgcggat
      361 gctgaaacaa aaaggatacc cgattgttga cgcggatgag atttcaaggc ttgtcgtggc
      421 accgggaagt cttgtgcttg atcaaattgc cacggctttc ggtaccgacg tactcctgcc
      481 ggacggatcg ctgaatcgag aaaagctcgg aagcctcatc tttaatgatg tggcaatgcg
      541 ggaaaagctg aacggaatca ttcatcctgc tgtccgggca gaaatgttac ggcaaaagga
      601 agaatggttg gaaaaagggg caaataccat catcatggac atcccgctcc tttttgaaag
      661 caaacttcag tcgtatgtag ataaaatcat cgtcgtttcg gcaacccccg tcatccaaag
      721 gaagcgcttg attgcccgaa acggctattc tgcggaagaa gccgatgctc gcatcaattc
      781 ccagctgccg atttcagaga aggaaaaagg cgcagatgca gtcattaata ataatggaac
      841 acttgatgag acggaagaac agctgaatgg gattttattg aaatgggatg caatacaatg
      901 aacttattct ctgaaaagtg gaacattgtt attttgttca caaactcttt gcacgtggtt
      961 aaaatgtgct atactaatta acggataaac gataataagt atataacatt acgcaggagg
     1021 attttttcat gactacttcg atagcgataa acggtttcgg gcgtattggc cgcatggtgt
     1081 tccgccaaat tatcacgaag gatgacgtgg atgtagtagc ggtaaatgca tcttatccgg
     1141 cagagacttt agcacatttg attaagtatg acacaaatca tggagttttt gacgggaaag
     1201 tcgttgtgga ggaaaatgct cttgttgtta acggaaagcg tgtccaatta atagctgagc
     1261 gcgatccgtc caagttgcct tggaaagagc ttggtgtaga tgtcgtcatt gaagctacgg
     1321 gagtattcaa tgaacgtagt aaagccgccc ttcatttgga tgcaggagcg aagaaagtca
     1381 ttttgacagc tccagggaaa aatgaagacg taacagtcgt tcttggtgtg aacgatgaca
     1441 aactagatat cgaaaaacat gatgtcattt ctaatgcaag ctgcacgacg aactgtcttg
     1501 caccggttgc aaaagtgttg aatgatacat tcggcattga aaacggattg atgacaactg
     1561 ttcatgctta tacaaacgat cagaagaact tggataatcc gcataaggac cttcgccgtg
     1621 cacgtgcatg tgcacagtcg atcatcccga cttctactgg tgcggcaaaa gcgttatcgc
     1681 tcgttttgcc ggagcttgaa gggaaaatcc acggaatggc gcttcgggta ccaacgccga
     1741 acgtatcact tgtcgacctt gttgttgacg tgaaaaaaga tgtaacagtg gaagaagtga
     1801 atgaggcatt catcaaagct gcagaaggcc cgctttcagg aatccttcgt ttcacgactg
     1861 aaccgcttgt atcgattgac tttaacacga caaccgaatc tgcaacaatt gacggtcttt
     1921 cgacgattgt catgggcgac cgcaaagtga aagtgctcgc ttggtatgat aatgaatggg
     1981 gctattcggc ccgcgttgtc gaattggcta taaaagtagg aaatgaattg aaatccaaag
     2041 tgaattctta attggaaaat gaaaaacgtc caagtcgatt tttcggcttg gacgtttttg
     2101 tccttttcta ataccaatgt tgattaagga tttataagta aggacccccg gcgaataact
     2161 atttcactga tgactctttg tacttctaat gctctcccta acaaagacaa tagccttttc
     2221 aacagttaac atatttccct tgtaccaatt ttcacccatt tctcttaccg acgg
//
LOCUS       DASULI010000243         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_43699, whole
            genome shotgun sequence.
ACCESSION   DASULI010000243 DASULI010000000
VERSION     DASULI010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43699"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1885)
                     /gene="ilvD"
                     /locus_tag="VFX34_05335"
     CDS             complement(218..1885)
                     /gene="ilvD"
                     /locus_tag="VFX34_05335"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5564360.1"
                     /translation="MRSDMIKKGIDRAPHRSLLYATGVKTKDLEKPFIGVCNSYIDII
                     PGHKHLNKFAEIVKEAIWEAGGVPFEFNTIGVDDGIAMGHIGMRYSLPSRELIADSAE
                     TVINAHWFDGVFYIPNCDKITPGMLMAAVRTNVPSVFVSGGPMEAGVSSSGAPLSLTS
                     VFEGVGAYKSGKMTAEELLDIENNACPTCGSCSGMFTANSMNCLMEMLGMALPGNGTI
                     VATSDERHRLIKEAARNLIRMVKEDVKPRDIITKEAIDDAFALDMAMGGSTNTVLHTL
                     AIAHEAEIEYSVQDINKVAERVPYLAKIMPASDISMDDIFKAGGVSAIINELTKIPGA
                     IHPDRITISGKTMREDVQDYHITNDQVIRTKDNPYSPVGGLSVLFGNIAPDGGVIKVG
                     AVDPSIKQFTGEAIVFESQEDAQENIDNGTVREGHVVVIRYEGPKGGPGMPEMLAPTS
                     AIQGRGLGTKVALITDGRFSGASRGISIGHISPEAAEGGPIALVEDGDIIDIDLTNRT
                     IELKVSDEELAERRAKLQPFEPKIKKGYLARYSKLVTSASTGGVMKI"
BASE COUNT          546 a          580 c          492 g          691 t
ORIGIN      
        1 tgttcgcggc atttgccgcc tcgtgaatgg actcaaatat cgagttccat tcgctgataa
       61 cgagcccaaa tttcatgggc ccgggattgc ctaatcagta accttttcag caatccactc
      121 cggggtgatg tcgcggatag ttgcattgcc ggcttccagc acgaccggct ctctgtgaat
      181 gcaaggatct atcccctttt accccatcat cgagttatta tattttcatg acgccgcccg
      241 tacttgcgga cgtcactagt tttgaatatc tggccaaata gccttttttg attttcggtt
      301 caaatggttg cagtttcgct ctgcgctccg ccaattcttc atctgacact ttcagctcaa
      361 tcgtccggtt cgtcagatcg atgtcgataa tatctccatc ttcgaccaac gcgattggac
      421 cgccttcagc cgcttccggt gaaatgtggc caatggaaat gcctcgggat gctccggaga
      481 aacgaccatc tgtaatgagc gccactttcg ttcctaagcc tcttccttga atcgctgagg
      541 tcggtgcaag catttccggc atgcccggcc cgcctttcgg accttcgtaa cggatgacga
      601 cgacatggcc ttctcggact gtgccgttgt cgatgttctc ctgcgcatct tcctgcgatt
      661 caaagacaat tgcttcaccc gtgaactgct tgatggaagg atcgactgct ccgactttga
      721 tgactccacc gtctggagcg atgtttccga ataggacgga caagccgcct accggactat
      781 atggattatc tttcgtccgg atcacttgat cgttcgtaat atgatagtcc tgaacgtcct
      841 ctctcatcgt ttttcctgaa atcgtgatac gatccggatg gatggctcca ggtatctttg
      901 taagttcatt gatgatggcg ctgacgccgc ctgctttgaa aatatcatcc atcgagatat
      961 cggatgccgg catgattttc gctaaatacg gaacccgctc agcaactttg ttaatatcct
     1021 ggacgctgta ttcgatttcc gcttcatggg caatcgccaa tgtatgtaga accgtattgg
     1081 tcgatccgcc cattgccata tcgagcgcga atgcatcgtc gattgcttcc ttcgtaatga
     1141 tgtcacgcgg tttgacatcc tctttcacca tgcggatcaa attccttgcc gcttctttga
     1201 tcagcctatg gcgctcatca gacgttgcga cgattgttcc gttgcccggc aatgccattc
     1261 ccagcatttc cattaggcag ttcatagagt tcgctgtaaa catgcctgag cacgagccgc
     1321 atgttggaca tgcattgttt tcgatatcaa gaagctcttc agctgtcatc ttgcccgatt
     1381 tgtatgcgcc caccccttcg aatacagatg tgagggatag cggagcaccg gatgaagaga
     1441 cacccgcttc catcggtccg cctgagacga atacggaagg gacattcgtc cgtaccgccg
     1501 ccatgagcat tcctggtgtg atcttgtcac agttcggaat gtagaacacg ccgtcgaacc
     1561 agtgtgcatt gatgactgtt tcagcagaat ccgcaatcaa ttcccggctc ggcagcgagt
     1621 aacgcattcc gatatgcccc atcgcgatgc catcatctac tccgattgtg ttgaattcga
     1681 atggaacacc gccagcttcc cagattgcct ctttaacaat ttcagcaaat ttattcaaat
     1741 gtttatgacc tggaattata tcaatatatg aattacatac accgatgaaa ggtttctcca
     1801 aatctttcgt tttgacgccc gtcgcgtaaa gcagactcct atgaggcgca cggtcgatcc
     1861 cttttttaat catgtcactt ctcattgcaa tctctcctaa tcaaatcatc caagagcttt
     1921 gaactgcgta aattgcgtga aatgaatcac atatctaaat agattgttgc tatcatatat
     1981 cggatatgtg cccacgtcaa ccgtcttttt ataattattg ttcaattatg aattatagtt
     2041 tattttgtat ccgcttacat tagcgatata ttgcatatta taggaattat gacattttaa
     2101 aaatattttt attttatttt tgatgaagaa acctgaaaac gcttttgctg gattgcaaaa
     2161 gcgcataaaa aagaatagac gcacataaat ttgtccagaa acgcataaat cgccacaaac
     2221 acgcataatg attttttttg cgcgactgga gcgatttatg cgtccttatt tggttttatg
     2281 cgtttttgcg gaagttatgc gctttttat
//
LOCUS       DASULI010000244         5705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1389018,
            whole genome shotgun sequence.
ACCESSION   DASULI010000244 DASULI010000000
VERSION     DASULI010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389018"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFX34_05340"
     CDS             <1..379
                     /locus_tag="VFX34_05340"
                     /inference="COORDINATES: protein motif:HMM:NF017328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ger(x)C family spore germination C-terminal
                     domain-containing protein"
                     /protein_id="HEX5564361.1"
                     /translation="YISLVMEKIKVKVKPVIVNGTAKFDIAVSADGVVDTITDGVTPK
                     EIEKLVKNEMEKQILTTYKEALNMDADIYRLSEHLYRKNVKQWKRLHKDGKVELTEDS
                     IQNITINIGKLASKRKTFERTIH"
     gene            464..1330
                     /locus_tag="VFX34_05345"
     CDS             464..1330
                     /locus_tag="VFX34_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEX5564362.1"
                     /translation="MKTQQKAPVWKLFARGAMVILGAVIAAYGLESVLIPNQVSDGGV
                     TGLSIVGSQLFGFPLGLLIAILNIPFVFLGYKQIGKSFALFSVLGIATLAISTSLMHH
                     VPTIIHGDTLLITVVGGIILGFGMGLALRNGGALDGIDMLAVLLSRRMPFGTSDLILF
                     LNVFVFAIVSTVFGLQGAFLSAIAYYIATKVISIVEEGLSGSKMYKIITSEPEDMVET
                     IRDRLGRSATYSIVQGAYSNEQFREITCIINRLEDSKMKEIILEIDPSAFVTVYEVAE
                     VRGGSFSKRNIH"
     gene            complement(1514..2128)
                     /locus_tag="VFX34_05350"
     CDS             complement(1514..2128)
                     /locus_tag="VFX34_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5564363.1"
                     /translation="MLKKLASDALGLSDIGKIIGPEDYDKTDADDYIRHEDDEKIYFL
                     IKTKADEYCFTNIAFIHVDGEKAVSSKRVLKRYPYSQYQFTNVLLETAGKVDLDVEIK
                     FTLGNQSFSIDVDKKQLAKLNDLYKALVYISEKAHENNLLFEMATQSLDKAATVLENS
                     RPSDIQLADQYKQLTEFGFSWLTSSRDKYHVKDFGDVFEKYINN"
     gene            complement(2424..4061)
                     /locus_tag="VFX34_05355"
     CDS             complement(2424..4061)
                     /locus_tag="VFX34_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564364.1"
                     /translation="MNLYHFEEIVSANIISQGIDYYKAGFVTDLARERGGRYTAVVKG
                     ADMYEIAVELDEEGNILSSYCDCPYIMGPVCKHEVAVYYALIDRLKDDDFPGEADHLD
                     LQAVLENLSKEELMTILIELAEKDPVLHNELILNYSFYDDEQREIGRCKRLMDSIIER
                     YMGREGFITYKHVGGFTEELSGVLHKTERLNDPLISFEIAQMLLGEAMKAFQYADDSN
                     GDIGYLIDETIKKMSAITREALDCNVQSELLDKLIHLSKSDIFEGWDDFRIDVLGICL
                     EFAGNEQMRKTLMNELESMIENSSANDYSRYANERIHNLLYDIIEIHGTAEEARQFLK
                     ENIKYPSFRKRLMQYEMKEGNYENVLALASEGERLDKDYRGLVTRWKKWRYKAYRQLN
                     SIDEQMKIGRELLIEGEFDFYRELKELASDETAFYEEVKQELAVKNNWMFVKLIEAEN
                     DVDAILAYVRENPSLIEQYLKYLVSAHKEEAIRLYERHVKEAAENSFNRKGYRAVCQV
                     LKRFGKVAGQNAQMKLAEELEQTYKNRPAFLDELGKL"
     gene            4221..4862
                     /locus_tag="VFX34_05360"
     CDS             4221..4862
                     /locus_tag="VFX34_05360"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5564365.1"
                     /translation="MKGSSHAIIGVTVGAIAAYQVQPDFYTLAIGSVVGGVSALVPDL
                     DTNGVASNRITLSKKFTQWLMQLVGIGILTAIVYQYFTTGYTPSIILYAGIGLLFFIV
                     SRFITQRRTLTLTGIMVMWLGIVMHQSIGILLAGSYIVIASFLPHRSYTHSLLGLAFY
                     AFILNHLQQEWAIEGLFIAGVAGYASHLVADMKLLPMNHRGVKWFLPFSNKEW"
     gene            complement(5183..>5705)
                     /locus_tag="VFX34_05365"
     CDS             complement(5183..>5705)
                     /locus_tag="VFX34_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HEX5564366.1"
                     /translation="LMNGNAESDGRQQELMDAFRSVQAAEETVEVLEKKAVAAKLDGV
                     EGILSIPAIDLEAPVLYGADAETLDRSLGAIENMDHPGKMNGSYAIAGHQAHAFGKYF
                     NRLHEMEVGTKFTFQTAEEEMAFKVFDIKIVKPHEVDVLEPKEGKALLSLITCYPAYS
                     NEYRLVVQGERVD"
BASE COUNT         1537 a         1395 c         1152 g         1621 t
ORIGIN      
        1 ctacatctcc cttgtcatgg aaaagataaa agtgaaagtc aaacccgtca ttgtaaacgg
       61 aacagcgaaa ttcgacattg ccgtatctgc cgatggagtt gtcgatacca ttaccgacgg
      121 ggtaacacct aaggaaatcg aaaaactagt gaaaaatgaa atggaaaaac aaatcctcac
      181 aacctacaaa gaagccctaa acatggatgc cgatatctac cggctatccg agcatctgta
      241 ccgaaaaaac gtaaaacaat ggaaacgact acacaaagac ggcaaagtcg aactgaccga
      301 agactcaatc caaaacatca ccatcaacat tggcaaactt gcatccaaac gaaaaacatt
      361 cgaacgcacc atccactgat ttccctgcga gggtgatgaa ctgacaacat gaagtttgta
      421 ggtgtaaaat ggagtaaatt gtttaaatgg ggaacgacgt atgatgaaaa cacaacagaa
      481 agcacctgta tggaaattat ttgcccgcgg cgccatggtg atacttggtg cggtcattgc
      541 agcatatgga ttggaatctg tgttgattcc caatcaagta tctgatggag gcgtgacagg
      601 tttaagcatc gtcggatctc aattattcgg ttttcctttg ggactgctca ttgcgatttt
      661 gaatattcct tttgtctttt taggctataa acaaatcgga aaaagtttcg ccctcttctc
      721 cgtacttggg atagctacac tggcgatcag cacgagcctg atgcaccatg taccgacaat
      781 cattcacggc gatacgctgc tgatcactgt tgtcggggga atcatcctcg gtttcggaat
      841 gggtttggcg ctgcgtaatg ggggtgcact tgacgggatt gatatgttgg ccgtcttgct
      901 ttcccggaga atgccattcg gtacaagtga cttgatcctc ttcttgaacg tcttcgtttt
      961 tgcaatcgtc tcgactgttt tcggacttca aggagctttc ctatccgcca tcgcttatta
     1021 cattgcgacc aaagtgatta gcattgtcga agaagggctg agcggttcca aaatgtataa
     1081 gatcattacg agcgagccgg aagatatggt ggaaacaatc cgtgaccgtc tgggccggag
     1141 tgcgacttac agtatcgttc aaggtgcgta ttccaatgaa cagttcaggg aaatcacatg
     1201 catcatcaac cggctggaag atagcaaaat gaaggaaatc attcttgaaa ttgatccgtc
     1261 cgcatttgtc acggtctacg aagtcgccga agtacgcggc ggcagcttca gcaaacgcaa
     1321 catccattaa cgaattgtgc gggaattgtg tgggtgcctg tgcaaggtgc aattatgaca
     1381 attcaacttg atttatataa atacaaaggg aagccgatca attgttgaat tgattggctt
     1441 ccctcttttc atttatacaa ttgtagttaa taaactgatt agtgtgctgc acaggcaccg
     1501 gttcaattcc ggttcagtta ttgatatact tctcaaacac atcgccgaag tctttgacgt
     1561 ggtatttgtc acgggacgat gttagccagg agaagccaaa ttcggttaat tgtttgtact
     1621 ggtcggccaa ttggatgtcg gatggtcttg aattctccaa aactgttgca gccttatcga
     1681 gactttgcgt cgccatctcg aataggagat tgttttcgtg ggctttttcc gaaatataca
     1741 ccaatgcttt gtatagatca ttcaatttcg caagctgctt tttatcgaca tcaatgctga
     1801 acgattgatt gccaagggta aacttgattt caacatccaa atcgactttg cccgccgtct
     1861 ctagaagcac attggtgaat tgatattgcg aatatggata gcgtttcaag acgcgtttcg
     1921 aagagaccgc tttctccccg tcaacatgga taaaagcgat attcgtaaag caatactcat
     1981 ctgcttttgt tttgattaaa aaataaatct tttcatcatc ttcatgtcgg atataatcat
     2041 ccgcatctgt tttatcataa tcctccgggc caatgatttt accgatatcc gacaaaccta
     2101 atgcatctga agccaatttt ttcagcattt gaccgacctc ttcctatagt aaaattcata
     2161 cacctaaatt atagcatctc ttcagttgaa tgaaatccat gaattcgaat tgtttctaaa
     2221 ttgttccggt gcctgtgcat ggcacattta tgacaattca acaggattta tataaatgca
     2281 aagggaagcc gatcaaatgt tgacttgatc agcttccctc ttttcataca tgcaattgta
     2341 gtgcattatc tgaatagtgt gttgcacagg caccttctcg tccgcgggca ccatctcgcg
     2401 caattggcac catctcgata gaattaaagc ttccccaatt catccagaaa agccggacga
     2461 tttttgtatg tttgttcaag ctcctccgcc aatttcattt gggcattctg tccggctact
     2521 ttcccgaagc gcttcaacac ttggcacact gccctatatc cctttcggtt gaatgaattt
     2581 tccgcagcct ctttcacgtg gcgttcgtat agccggatcg cctcttcttt atgtgcactt
     2641 acgagatatt tcaaatactg ctcaatcaat gaaggatttt cgcgtacata tgcaaggatg
     2701 gcatcgacgt cgttttcagc ctcgatgagc ttaacaaaca tccaattatt tttcacagcc
     2761 aattcctgct tcacttcttc ataaaaagcg gtttcatccg aggcaagctc ctttaattcc
     2821 cgataaaaat caaattctcc ttctatcagc aattcacggc ctattttcat ttgctcatca
     2881 atagaattca attgtcgata cgccttatag cgccatttct tccaccgcgt caccaagcca
     2941 cgatagtcct tgtccaatct ttccccttca gacgccagtg ccaagacgtt ttcatagttg
     3001 ccttccttca tttcatattg catcaaccgt ttccgaaatg atggatactt gatgttctcc
     3061 tttaaaaact gtctcgcttc ttcagctgtc ccatggattt caataatgtc atacagtaag
     3121 ttgtggatcc gttcatttgc ataccggcta taatcatttg cggacgaatt ctcgatcatt
     3181 gattccaatt cattcatcaa cgtcttgcgc atctgttcat ttcctgcaaa ctccaaacag
     3241 atgccaagta cgtcaatccg gaagtcatcc catccttcaa atatatcgga tttcgataaa
     3301 tgaatgagtt tatccagcaa ctcgctttgc acattacaat ccaaagcttc acgcgtgatc
     3361 gcagacatct ttttgatcgt ttcatctatt aaataaccga tatcgccatt cgaatcatcc
     3421 gcatattgaa aggctttcat cgcttccccc aacaacatct gcgcaatctc aaaggaaatc
     3481 aaaggatcat tcaatctctc tgttttatgg agcactccgg acaattcttc cgtaaagccg
     3541 ccgacatgtt tatatgtaat aaacccttcc ctgcccatat atctctcgat aatcgagtcc
     3601 atcaaccgct tgcagcgacc aatttcccgc tgctcgtcat cgtagaagga gtagtttaaa
     3661 attaattcat tatgtaaaac cggatctttt tccgccaact cgatcagtat agtcatcagt
     3721 tcttctttgg agagattttc aaggacggcc tgtaaatcca gatgatcggc ctccccggga
     3781 aaatcatcat ccttcagcct gtcaatcaat gcatagtaaa ccgcaacctc atgcttacaa
     3841 accggcccca taatatacgg acaatcacaa taagacgaaa ggatattgcc ctcttcgtcg
     3901 agctcgacgg caatctcata catatccgcc cccttgacga ccgctgtgta tcttccgccc
     3961 ctctcacggg caaggtcagt aacaaatccc gctttataat aatcaatacc ttgactgata
     4021 atattagcac ttacgatctc ttcaaaatgg taaagattca tcacacatca ccctctcctc
     4081 atccggtgcg cacacttcca ctataccaaa tacgacacct atccaaaaag tccttcatta
     4141 aagtgctaac ttcagcccat tgcctcccct acccggcgta agggataata agaggattcc
     4201 acaacgagag aggtttacag atgaaaggca gttcacatgc aattatcggc gtcactgtag
     4261 gggcgattgc agcctatcag gtgcagcccg atttctatac actcgcgatc ggctctgtgg
     4321 tcggcggcgt gtcggcgctt gttccggact tggacacaaa cggtgtagca agcaaccgga
     4381 tcaccctatc caagaaattc actcaatggc tgatgcagct cgtcggaatc ggaattttaa
     4441 cggcgatcgt atatcagtat ttcacaacag gctacacccc ctccattatt ctttacgcgg
     4501 gaatcggctt gctctttttc atcgtatcgc gtttcattac acagaggaga acgttgacgc
     4561 tgacgggcat catggtgatg tggcttggaa ttgtgatgca tcaatcgatc ggcatcctgt
     4621 tagccggaag ctacatcgtc atcgcctcat tccttccgca ccgatcctac acccactcgc
     4681 tcctgggcct agcattttat gcgttcattt tgaatcactt gcaacaggag tgggcaatcg
     4741 aaggactttt cattgcggga gtagccgggt atgcaagcca cctggtcgcg gacatgaagc
     4801 tattgccgat gaaccaccga ggcgtaaaat ggtttctccc attctcgaac aaagaatggt
     4861 gaattgtgtc caatttgcgc cggtgcctgt gcaaggtgca tttatgacaa ttcaactgat
     4921 agtattaaaa tacaaaggga agtcaaccga aactaatctg gcagacttcc cttttctacc
     4981 ttatacaatt gtagttaata aacagaattg ttccggtgcc tgtgcaagaa acatttatga
     5041 caattcaata gtatgtatac aaatacgatg aaaagccgat caattattca tttgatcggc
     5101 tttccgtttc ctcattatac aattgtagtt aataaactga aaagtttcct gcacaggcac
     5161 ccacacaatt cccaaacaat tttcaatcaa ccctttcccc ctgaacgacg agtctgtatt
     5221 cgttggaata cgcagggtag caggtgatga gagacagaag agctttacct tcctttggct
     5281 caaggacatc gacttcgtgc ggcttgacga ttttgatgtc gaataccttg aacgccatct
     5341 cttcttcagc tgtttggaat gtgaacttgg tgccgacctc catttcatgc agccgattga
     5401 aatacttgcc gaaagcatgg gcttgatgcc cggcgatggc ataactgccg ttcatcttgc
     5461 cgggatggtc catgttttcg atggcaccga gggatcggtc aagcgtctct gcatccgcgc
     5521 cgtacaggac cggcgcttcc aggtcgattg ccgggattga taagatgcct tccaccccat
     5581 caagtttggc ggctacggct ttcttttcaa gcacttcgac cgtctcctcc gcagcttgaa
     5641 cggaacggaa tgcatccatc aattcttgct ggcgcccatc actttctgca ttgccgttca
     5701 tgagc
//
LOCUS       DASULI010000245         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_977564, whole
            genome shotgun sequence.
ACCESSION   DASULI010000245 DASULI010000000
VERSION     DASULI010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977564"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFX34_05370"
     CDS             <1..538
                     /locus_tag="VFX34_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564367.1"
                     /translation="EMLVVEPLFIEDIIERMIRAGMIVKSGGAYSLTETGKQQLEAGI
                     YVQPPEMEESTVHYSPSHGRFLVGETADSAGEPYRHAGDTRKLKDFRDDEWREALDQL
                     DVAYMDGNVQLVVQAIAAVNELEQKSAPCIEFRLHQTADDRLYARIWNTMTGQWDKTL
                     ENQIMERELSEWREKYLG"
     gene            complement(677..1099)
                     /locus_tag="VFX34_05375"
     CDS             complement(677..1099)
                     /locus_tag="VFX34_05375"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX5564368.1"
                     /translation="MKKTFIGSFPNHERLLSKILELKHEGVEEQDLHIVMKDELAIDE
                     LRSSALQEGEDSPYNLFNHFMGFLAGEHNVRRLLKDSGFTENEAKHYFDAVQEGALLL
                     YMNGKLKKKLSDEPIAVERQYEGYKPIPLDEPEASPDI"
     gene            1252..1989
                     /locus_tag="VFX34_05380"
     CDS             1252..1989
                     /locus_tag="VFX34_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564369.1"
                     /translation="MESSKVHHRDKPTGFPANQDRFVWNPYVEFIREQNGVNAQLMAS
                     VEKLERLCGKILDIVSNQQHVHRNRYFHLRDRIWEVHEKLRAESVRQDAIREELGKQG
                     EAVFRLRKSLQNHRMSMREFTIHQYDDLHDVLDMLDKISTDHVKIGEMQEKVINKLEE
                     QEVQRKREAEEVEKSIGKILLAKKSIGTLLATLPPTYPIQQIVVDGGTIDVDHLLNVD
                     ETKGVAYFSSSTGIVTVSIDKLDAIHW"
     gene            2154..2393
                     /locus_tag="VFX34_05385"
     CDS             2154..2393
                     /locus_tag="VFX34_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5564370.1"
                     /translation="MAKYTVVNQQTCIACGACGASAPGIFDYNDEGLAFVRLDDNKGS
                     AEVDEHLYDDLEDAWEGFPTDSIKVSQHPIILEEA"
BASE COUNT          737 a          559 c          688 g          661 t
ORIGIN      
        1 cgagatgcta gtcgtcgaac cgttattcat tgaagatatc attgaacgga tgatccgggc
       61 gggaatgatt gtgaagagcg gcggcgccta ttcattgacg gagacgggta aacagcagct
      121 cgaagccggc atttacgtcc agccgccgga aatggaggaa tcgacagtcc attatagtcc
      181 gtcgcatgga agattccttg tcggggagac ggcggatagt gctggtgagc cgtaccggca
      241 tgcaggggat acccgcaagc tgaaggattt ccgtgatgat gagtggcggg aagcgctcga
      301 tcaattggac gttgcttata tggatggcaa tgtccaactc gttgtccaag cgattgcagc
      361 agtcaatgaa ctcgaacaaa aatccgcccc ctgcatcgaa ttccgtcttc accaaaccgc
      421 cgatgaccgg ctttatgcgc gcatctggaa cacgatgacg ggtcagtggg ataagacgct
      481 tgaaaatcag atcatggagc gcgaactatc ggagtggcgg gagaagtatc tggggtgacg
      541 gtgcagaccg cctgaagtcg gactgttacc gcctaaagat tcgcttgctc cgcctgaacg
      601 agcaccgcaa ccgcctaata tctcaactgt tacgattgaa aagctgaagg atttcccttc
      661 agctttttct aatacatcaa atatcaggac tcgcctctgg ttcatccaac gggatcggct
      721 tatagccctc gtattgcctt tcaaccgcta tcggctcatc gctaagcttt tttttcagct
      781 taccgttcat gtacagcagc aacgcacctt cctggacggc atcgaaataa tgttttgctt
      841 cattttcggt gaaaccggag tccttcagca agcgtctcac gttatgttca ccggcaagga
      901 atcccatgaa atgattgaaa agattgtacg gagagtcttc gccttcctgc agcgcactgc
      961 tccgtagttc atcgatcgcc aattcatcct tcatgacaat atgcagatcc tgttcctcca
     1021 caccttcatg tttcagctcc agtattttag acagtaatcg ttcgtgattc ggaaatgatc
     1081 cgataaatgt tttcttcatc atggtacctc cttaaaccta tttgtcttcg gaaaataccc
     1141 tcgttgcagg aaaaggaaac aaccaaggtt gccgccttcc ggtaaaacgt gaacaatagg
     1201 cggtccgggt gacatagtat aggagaaaga ctcggtagag gagcaagaga aatggaaagt
     1261 tcgaaggttc accatcgtga caagccgacc ggttttcctg cgaatcaaga tcggttcgtc
     1321 tggaatccgt atgtcgaatt cattagagaa caaaacggtg tcaatgcgca gttgatggca
     1381 tcggttgaaa agttggagag gctttgcggc aagattctgg atatcgtttc caatcagcag
     1441 catgtccatc ggaaccgcta ttttcattta cgggacagga tttgggaagt ccatgaaaaa
     1501 ctgagggcgg aatccgtcag gcaggacgcc atacgggaag agctcggtaa acagggggaa
     1561 gctgttttca ggctgcgtaa aagcttgcaa aaccatcgga tgtcgatgcg tgaatttaca
     1621 atccatcaat atgatgatct gcatgatgtg ctggacatgc tcgacaagat cagtaccgat
     1681 cacgtaaaaa tcggggaaat gcaggagaag gtcatcaata agttggagga gcaagaggtg
     1741 cagcggaaac gggaagcgga ggaagtggag aagtcgatcg gaaaaatcct tctggcaaag
     1801 aaaagtatcg gcacgctgct tgccacgttg ccgccaacgt atccgatcca acaaatcgtg
     1861 gtggatggcg ggacgattga tgtggatcat ttactaaacg tggatgaaac gaagggggtc
     1921 gcctattttt catcctcgac cggcatcgtg acagtgtcaa tcgataagtt ggatgcgatt
     1981 cattggtgag gcggattgac gttcaaatcc ggatcaaaag ttcaacctgg gctgcaagat
     2041 agtggcctgg gttgaactgt ttttttataa gtactatcta ttcatttagt tgaatatcat
     2101 gcaacgtaaa tgaaacgaat gacgaaaata tgacatatgg agaggatgac aaaatggcta
     2161 aatatacggt agtgaatcag caaacctgca ttgcctgcgg agcgtgcggg gcgagtgccc
     2221 caggcatttt tgattataac gatgaagggc ttgcgtttgt acgtttagac gataataaag
     2281 gatctgccga ggtagatgaa catctatacg atgatctgga agatgcttgg gaaggcttcc
     2341 ccaccgattc aataaaagtg tcgcaacacc ctattatcct ggaagaagca tagtagacaa
     2401 gagcagaagt taaatttatc agaatacatt tgcttttaaa atatctatgt attatactgg
     2461 ctttatatat aacactatta ttatcaatcg ttcacatgct gttatgctag acggagagat
     2521 ttcatacctt aggcaaaggg ggattgtata tgtctacttt acacaatacg gctgaagagg
     2581 agcagttagc actctttatg cagcgtattg aggcggggga taagattgaa gcggatgatt
     2641 ggatg
//
LOCUS       DASULI010000246         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_376495, whole
            genome shotgun sequence.
ACCESSION   DASULI010000246 DASULI010000000
VERSION     DASULI010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376495"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="VFX34_05390"
     CDS             complement(<1..1787)
                     /locus_tag="VFX34_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5564371.1"
                     /translation="MKNKVLLLLWATLVTIVLLASLSFARQGTGLIGKDFSDSDDMKW
                     RLEEFYNVIGPSVLNPIDKEEAKKKITVSEREIEEHRTRYGTLADQLANIHDQYAQRI
                     TDATNASNDVLRETLIEERDMKLEDIRKNFEGNAHVEAKIRKAKEKVIENYLNEIINK
                     SYSLHVAYELKNVETGETFSHGDISKPAVYKKKFNANGDYLKASSLPGRNTVWTTPYI
                     NYYEDTHVSDEEITAMANPVQSFEGVVIVPKATFEKGGALYAQYKEFNRGKYGMYAFW
                     ALGLLALIALLTVMKFRKEWVTETPIADWYSDVKIDVKAAAFLMSALILVEYINSESY
                     NLRNLFTHLRLENLVIWLGTFFVGGIAMTALLAFQLVNGVERWRQEGVFVKDVKSSFT
                     LQFFKAWREMFLKKSIGVQSFILLIGFFLAGIGFVGGFIAPFLFIVYIACVLFLGLPA
                     LYVFIRRSAYLNRIIVATEQMASGRLNEDIKIEGRSPLADHARNLNNLREGVRVSMSE
                     QAKSERLKTELITNVSHDLRTPLTSIITYTDLLKDETITPEERAKYVDILDKKSQRLK
                     TLIEDLFEVSKMASGNLELHKQRVDLTQLL"
     gene            complement(1787..1966)
                     /locus_tag="VFX34_05395"
     CDS             complement(1787..1966)
                     /locus_tag="VFX34_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564372.1"
                     /translation="MATKSRSDWGVVLSIFSICIAMVSIGYCFPHILDMIESGWERSS
                     RAIAYFTRIIRGGAA"
     gene            complement(1944..2633)
                     /locus_tag="VFX34_05400"
     CDS             complement(1944..2633)
                     /locus_tag="VFX34_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5564373.1"
                     /translation="MAKFTVLVADDDKEIRDGIEIYLKNEGYSVLKAADGKEALDLIQ
                     TNEVHLLILDIMMPNMDGITATFKIREVQNIPIIMLSAKAEDTDKIHGLSVGADDYVT
                     KPFHPLELMARVKSQLRRYVQFGTFEGRKKIEIDGLALDEDAKELSVDGKPVKLTPIE
                     YKITELLMKNAGRVFSINEIYERVWNEEAYNAENIVAVHIRKIREKIEADPKNPRYVK
                     VVWGIGYKIEK"
BASE COUNT          744 a          744 c          541 g          803 t
ORIGIN      
        1 agcaattgcg tcaaatcgac acgctgctta tgcagctcca aattgccgct cgccattttc
       61 gacacttcga ataaatcttc gatgagtgtc ttcaatcttt gcgatttctt atccaatatg
      121 tcgacatatt tcgcccgctc ctctggtgta atcgtttcat ccttcaaaag atcggtgtac
      181 gtaatgatcg atgtcaacgg cgttcgcaga tcatggctca cattcgtaat caattctgtt
      241 ttcagccgct cgcttttcgc ttgctcactc atggaaaccc gcacgccttc acggagatta
      301 ttcaaattcc ttgcatgatc cgcgagtggc gaccggcctt cgattttgat gtcttcattc
      361 aaacgtccgg aagccatttg ttctgtcgca acgatgattc ggttcaagta cgcagaacgc
      421 ctgatgaaaa catagagcgc aggcaagcca aggaatagga cgcaagcaat atagacgatg
      481 aataaaaatg gagctatgaa tcctccgacg aatcctatac ccgcgaggaa aaatccgata
      541 agcaggatga acgactgaac gccgatcgac tttttcagga acatctcacg ccatgcctta
      601 aagaactgta gcgtaaaact gcttttcaca tccttcacaa aaacaccttc ctgcctccac
      661 cgttccacgc catttacaag ctggaatgcg agcaaggcag tcatggcgat gccgcccaca
      721 aagaaggtgc ctagccaaat aaccaaattc tccagtctta agtgcgtaaa taagttccgc
      781 agattgtagg actcgctgtt aatgtattct actaaaatca gcgccgacat caaaaatgct
      841 gcggctttta catcgatttt tacatcagag taccaatcag caatcggtgt ttctgtcacc
      901 cattccttcc gaaacttcat aacagttaat aaggcaatga gtgctagcaa cccaagagcc
      961 caaaaggcat acatgccata tttgcctctg ttgaactcct tgtattgggc atataaagca
     1021 ccgcctttct caaaggtcgc tttcggaacg atgacaacac cttcaaagga ttgcacggga
     1081 tttgccatag cggttatttc ttcatcagac acatgagtat cttcataata attaatgtat
     1141 ggcgttgtcc acaccgtatt gcgtcccggc aatgagctcg cctttaagta gtcaccattt
     1201 gcattgaact tctttttata tactgcaggc ttcgaaatgt caccatgaga aaacgtctcg
     1261 ccagtttcaa cgttctttaa ttcataggcg acatgcaatg aataggactt attaataatt
     1321 tcattcagat aattttctat caccttctcc ttcgccttac gtattttggc ctctacatgc
     1381 gcattgccct caaagttctt tcggatatcc tccaatttca tatcccgttc ttcaatcaac
     1441 gtctccctta atacgtcatt actagcattc gttgcatcgg taatcctttg tgcgtattgg
     1501 tcatggatat tggcaagctg gtcagccagt gtgccatagc gagtgcggtg ctcctcaatt
     1561 tccctttccg atactgtaat tttcttcttc gcctcttctt tgtcgatcgg gtttaaaacg
     1621 gaaggaccaa ttacattata aaactcttct aaccgccact tcatatcgtc cgaatcggaa
     1681 aaatcctttc caatcaaacc cgttccctgt cttgcaaacg aaaggctggc aagcagcaca
     1741 atcgtaacta gcgtagccca tagtagcaaa agaactttat ttttcatcag gccgcgcccc
     1801 ctctaataat gcgtgtgaaa taggcaatcg cccgtgaaga tcgttcccat ccgctttcga
     1861 tcatatcaag gatgtggggg aaacaataac cgatgctgac catagcgatg caaatggaga
     1921 atatcgacaa gacgacgccc caatcacttc tcgattttgt agccaatgcc ccacaccacc
     1981 tttacatatc tcggattttt cggatccgct tcgattttct cgcgaatttt gcggatatgg
     2041 accgccacaa tattttccgc attgtacgct tcttcattcc acacacgttc gtaaatttca
     2101 tttattgaaa acacacgccc cgcgtttttc ataagcaatt ccgtaatctt atattcaatc
     2161 ggcgtcagtt tcaccggttt cccatcgacc gataactctt tcgcatcttc atcgagcgcc
     2221 aacccgtcaa tctcaatctt tttccggcct tcgaatgtac cgaactggac ataccgacgc
     2281 agctgcgact tgacgcgcgc catcaattca agcgggtgga acggcttcgt cacataatca
     2341 tccgcgccga ccgacaatcc atggatctta tccgtgtcct ccgcctttgc actgagcatg
     2401 atgatcggga tgttctgcac ctcccggatt ttgaacgtcg ccgtaatccc atccatattc
     2461 ggcatcatga tgtcgaggat gagcagatgg acttcattcg tttgaatcaa atcaagcgcc
     2521 tctttcccat ccgccgcctt caacacgctg tatccttcat tttttaaata aatttcaatc
     2581 ccatcgcgga tctctttatc atcatccgcc acgagaactg tgaacttcgc catcatccgc
     2641 acctcacttt cctctatgat accaattgta gtggataaac cttaacattc gatgagggga
     2701 attatgaaga aattcttaag aaagtcgttt actctgaaac aaggcgggtg ttgaaatgac
     2761 actgggcggt gagaaatacc gtctgagtgg taataagctt ctgccggctt ggaaaattgg
     2821 cgcatttgca tg
//
LOCUS       DASULI010000247         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_178816, whole
            genome shotgun sequence.
ACCESSION   DASULI010000247 DASULI010000000
VERSION     DASULI010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178816"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFX34_05405"
     CDS             complement(<1..490)
                     /locus_tag="VFX34_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564374.1"
                     /translation="MVYSVCCLQRNILKRTVFRWHPDICEFIISKMQNPRILRYLIEN
                     TEDEMIKKLANEKLKFKPLTAQEEAMYQGILNYRSIPGMGGFNEAILKDAETKLRDGG
                     TYSVHNPEFLTGANISVTLTDDFMKAVEQDADYELRFPAVEKYSPEEMAAYNEEWHKV
                     GDV"
     gene            complement(769..1590)
                     /locus_tag="VFX34_05410"
                     /pseudo
     CDS             complement(769..1590)
                     /locus_tag="VFX34_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
     gene            complement(1759..>2350)
                     /locus_tag="VFX34_05415"
     CDS             complement(1759..>2350)
                     /locus_tag="VFX34_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEX5564375.1"
                     /translation="VLHEHELTYSVIVTEEYPGMPETVTEAYRVISGGLLEGFRNLGL
                     HAEFSVPVSKEQSEGLKKPKSAVCFDAPSWYELVVEGKKVAGSAQTRQKGVILQHGAI
                     LLGLDEDKLVSLFKFPSEEIRKRMRDALPEKAVAIDRLTDRDISVDECVEAFAKGFET
                     ALDIVLQPIELTEEQLLYVEEIEQRKYANKDWTFKK"
BASE COUNT          565 a          599 c          466 g          720 t
ORIGIN      
        1 gcacatctcc gactttatgc cattcctcat tataagctgc catctcttca ggagagtatt
       61 tttcaactgc agggaaacgt agctcataat ctgcatcttg ctcgactgct ttcatgaagt
      121 cgtctgtcaa tgtgacggaa atattcgcgc ccgtcaggaa ttcaggattg tgtactgaat
      181 acgtcccacc atcacgcaat ttcgtctctg catcttttaa aatcgcttcg ttgaaaccgc
      241 ccatacccgg gatgctgcga tagtttagga ttccttggta catcgcttcc tcttgcgcag
      301 tgagcggttt gaatttcagt ttttcatttg ctaacttctt gatcatttcg tcttctgtgt
      361 tttcgataag ataacgcaga atccttggat tttgcatttt tgaaatgata aattcgcaaa
      421 tgtcaggatg ccacctaaat accgtccttt tcaggatatt tctctgcaag cagcagacgg
      481 agtagactat atcatcaccc tcagcttcat ctgttagggg tcgggcgctt cgggaagaac
      541 tgtacttccc tactctactt ccattcatta tgaatgtttt cgatagtcgt tgaaccttca
      601 cctaaaacgg tgcttggctg cggattgtcc aatgccatta atttttaagc attcacgccc
      661 atcgtttccg atcacgttgt agcttaatgg cctctaagga gttcttcgca attcacccga
      721 ttttctagtc atccattgtc tggacgacgc ggactagcgt attaattaat ccgctaacat
      781 aatcatctgg gcaccacgtc tcgatccacc ttgttcgaca agatgtgtca atttcgcaat
      841 atcatcaagc catgagactg agccggatga tttaccattg acgcctcttg ctaatgtgtt
      901 gcgcggtcgt aatgtggaac cgttcgttcc tactccgccg ccacggctca tgatttccat
      961 cacctgttta cggtgatcgg agatgccttc acgggaatcc gccacgaacg gcatgacgta
     1021 acagttgaaa aatgtcactt ctgtatcaga tccagccccg tacaagacac ggcccgctgg
     1081 aatgaagttc agtctggaga gttgtccata aaactgatca aatgatttct tccgctgctc
     1141 ttccgtcttt tcaacagaag caagccctgt cgcattccgt tttgcaatct gctcgtaaaa
     1201 cacttcaaga ggcttttcga tcacatcgat cggccttgta atgatgccag tttcttgatt
     1261 ttcagggtta tcgattgcac tgcggtattc cttttcgatc caaatatcta ctgttcgact
     1321 tttctcgtcg agagctgtga tgaagccaag ccccctcgca ggaaacttcg gatcctcctt
     1381 caccgtcagg acaacgaaat caccgacctt cagtgttttc ttttccgtat ccttgaaaga
     1441 atatcggtcg atcatgacga gcctcgagac accgctgtgt tcgattttca tgtcttctgt
     1501 aattggatgc acttgcggga attcggcaat atcctcattc aaccgcgcct tgtccagaat
     1561 tggttcctga tgctttgaaa caattaccat tgacgcatcc tccttctggt atatttttgt
     1621 caacacaaca tgttgtgttg aatctattcc ctaatctact acatattgaa acctgtcgca
     1681 atgataagat ttagaaccgg aaaatccgaa gttgatatta tgcggtttac cacaaaaaat
     1741 aatccgaaag ttccagtctc attttttaaa cgtccagtct ttgttcgcat atttcctttg
     1801 ttctatttct tcgacataaa gcagttgctc ttctgtaagt tcgatcggtt gaagaacaat
     1861 atccaaagct gtttcgaagc ctttcgcgaa agcttcgaca cattcatcca cagaaatatc
     1921 ccgatcggtc agtcgatcga tggcgaccgc tttttcgggt agggcatcgc gcatcctttt
     1981 ccgaatttct tcagatggga acttgaataa cgagacgagc ttgtcctcgt ccaaacctaa
     2041 caggattgct ccgtgctgta aaatcacacc tttctgccgg gtttgggcac tccccgccac
     2101 ttttttccct tctacgacaa gttcatacca gctaggtgca tcgaaacaaa cagcactttt
     2161 cggctttttc aacccttcgc tttgttcctt cgacacaggt acagaaaact ccgcatgcag
     2221 accgagattt cggaagcctt ctaacagacc gccggagatg acccgataag cctctgtaac
     2281 tgtttccggc attccgggat attcttctgt aacgatgacg ctatatgtaa gttcgtgctc
     2341 atgcagaaca
//
LOCUS       DASULI010000248         4786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1696761,
            whole genome shotgun sequence.
ACCESSION   DASULI010000248 DASULI010000000
VERSION     DASULI010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696761"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFX34_05420"
     CDS             <1..185
                     /locus_tag="VFX34_05420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391562.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5564376.1"
                     /translation="FHVAEQFTGQKGSYVPVAETVQGFKEILEGRYDHLPEDAFRLVG
                     RIEEVIEKAKGMGVEV"
     gene            210..611
                     /locus_tag="VFX34_05425"
     CDS             210..611
                     /locus_tag="VFX34_05425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX5564377.1"
                     /translation="MKTIKVNIVTPDGPVSETEADMVIAATETGEIGILPGHIAMVAP
                     LQIGALRLKKDNSTVNVAVHGGFIEVRPDVVTVLAQHAELASDIDIARAKRAAKRAEE
                     KLQAKTDKLEAQLAELDLRRAINRINVYEQK"
     gene            1060..1296
                     /locus_tag="VFX34_05430"
     CDS             1060..1296
                     /locus_tag="VFX34_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HEX5564378.1"
                     /translation="MNDVLAHNPMLAIVSHIFFIGLSFYALQAIMPEKIIKKNHVFQA
                     QLLFILLSFAIGSTVSNFFLDISYWSGRIPNLFN"
     gene            1703..3004
                     /gene="murA"
                     /locus_tag="VFX34_05435"
     CDS             1703..3004
                     /gene="murA"
                     /locus_tag="VFX34_05435"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5564379.1"
                     /translation="MDKIIINGGRVLNGHVRVEGAKNAVLPILAAALLASDGPNIIRD
                     VPNLSDVSTINEVVKSLNAKVEYDPSRGQVIIDSTEELASEAHFDYVRKMRASILVMG
                     PLLARNGFARVALPGGCAIGSRPIDQHLKGFEAMGAEITFGHGHVEAKAENGLTGAKI
                     YLDFPSVGATENIMTAAALAKGTTIIENAAKEPEIVDLANFINEMGGRVVGAGTDAIR
                     IEGVEKLHGTIHHIIPDRIETGTFMVAAAITGGDVTIENAVPEHNAALISKLGEMGVI
                     ITELDEGLRIRAKHPLKAVDLKTMPHPGFPTDMQSQMMALMLTASGTGVLTETVFENR
                     FMHVEEFRRMNASVKIEGRSVIISGPSNLQGAEVAATDLRAAAALILAGLVAEGVTRV
                     TELDHLDRGYVDFHKKLKALGADIERVSTEEKKTEIEKQLV"
     gene            3077..4075
                     /gene="spoIID"
                     /locus_tag="VFX34_05440"
     CDS             3077..4075
                     /gene="spoIID"
                     /locus_tag="VFX34_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HEX5564380.1"
                     /translation="MNETRGFIIQSNMDKLLAVLFIVLLFFIPILMKPTAVPKENEAM
                     EDPCAITITVEGVDKPMPLEEYVVGVVAGEMPIDFHDEALRAQAIAARTYVLRATDGG
                     KKPIGATVSAQVYKTAAERKERWGKKFKQNEKKLRGIVATTEGDTIVYGDEMITAMFF
                     STSNGKTETAKNYSGSQIPYLQSVESPGEELVAAKVERKIEMPISEWNEALGSVWKAD
                     NFKSLQLVRNQTGRVQKAVSTGFEMSGRDMREALGLPSTDFNIAYDVTNQIVLITTKG
                     YGHGVGMSQYGAEAFARKGWTAENILTYYYSGTTIKKFEVDESECLKTPTLANNSK"
     gene            4086..4739
                     /locus_tag="VFX34_05445"
     CDS             4086..4739
                     /locus_tag="VFX34_05445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5564381.1"
                     /translation="MREEKPKSPSQKNKSQKNRWFWPAIYAGIAIVFVGMIWGYNALI
                     QKGPNEQADVATDPNAPPLVETNASNEVLKFPFNEDQLDSMAIIQDYYDVEASEEDRE
                     NALLVFNQTYYTNKGVSISMEGQPFEVVAAMSGTVTEVVIDQFKGAEVKIEHANGLTT
                     LYGSLTGILVKEGDEVVQGQSLGTATSNEWNAKAGTHLHFEVHKDGNPINPRSMLGF"
BASE COUNT         1408 a         1141 c         1195 g         1042 t
ORIGIN      
        1 acttccacgt tgccgagcag ttcacaggcc aaaagggttc ttacgtgccg gttgctgaaa
       61 ctgttcaagg cttcaaggaa atcttggaag gccgctatga ccaccttccg gaagatgcat
      121 tccgcctcgt cggacgcatc gaagaagtta tcgaaaaagc aaaaggcatg ggtgtagaag
      181 tataatctac aggatcagga gggaaacaaa tgaagacaat caaagtcaat atcgtcactc
      241 ccgacggacc tgttagtgaa actgaagcgg atatggtgat cgccgccacg gaaactggtg
      301 aaatcggaat ccttccgggc catatcgcaa tggttgcacc actccagatc ggtgcccttc
      361 gcctgaaaaa agacaactcg actgtaaatg tcgccgtcca tggcgggttc atcgaagttc
      421 gtccggatgt tgtcaccgtt ttagcacaac atgctgaatt ggcttccgac atcgacatcg
      481 cccgcgccaa acgcgcagcg aaacgtgcgg aagaaaaact acaagccaaa acagacaaac
      541 ttgaagcgca gttggcggaa ctcgacctca gacgggccat caaccgaatc aacgtttacg
      601 aacagaaata atagatgtga ctagttggcg ggcagaggga gtctttcttc tgcccgcatt
      661 ttcttgactg ggaaacttag ctaggcgagg cattttcagg ttgaataaat tgacgcgctc
      721 ataaacagcg aactcgcgct caacaaatcg ttttcgcgct cataaacggc ggaattgcgc
      781 tcaacaatcc gatttcgcgc tcataaaccg tgtgattgcg ctcaaaaatc cattttcgcg
      841 ctcataaacc atgagaatgc gctcaacaat ccgatttcgc gctcataaac catgggaatg
      901 cgctcgacaa tccgatttcg cgctcataaa ttgcggaatc gcgctcaaca atccgttttc
      961 gcgctcataa accattggaa tgcgctcaac aattcatttc cgcgctcata aatcgcgaca
     1021 cacgcttata aattcagttt tccaaaagga ggcaggacta tgaatgacgt tcttgcacac
     1081 aacccgatgc tggccatcgt ctcgcatatt tttttcatcg gcctgtcatt ttatgcgctg
     1141 caagcgatca tgccggaaaa aatcattaaa aagaaccatg ttttccaagc gcaactgctc
     1201 ttcattttat tgagttttgc aattggttcg accgtatcaa atttcttttt ggacatatcc
     1261 tattggtcag gaaggattcc aaatttattt aattgacatt gtttggatgg gtgaaaaacg
     1321 ggtacgtagt cagtaggcta acgtcacagt aggaactgcc catatgccac tggatccatg
     1381 tcatcgccgc gtctacatac ggatgtgtac ggggcgatgg catgaaatga aataaatgtt
     1441 taattcccga cagtactcgg gaattacatt aagtgtcaat tgacaattgc ctacctagga
     1501 aagcacacaa atcgacaaat gggtacatat tatagactat cttgtagata gacgggaaaa
     1561 atagctctgt agttacttgt ttaataatgt agagcattgt agaatagtat gtgtttttat
     1621 gtttaatttt atgacgaaaa aaatcaagtg aaaagaaaat agtatggcaa acagtataaa
     1681 ttttgagtcg gaggggcttt ttgtggacaa aataattatc aacggtggcc gtgtcctaaa
     1741 tggacacgtg cgtgtggaag gcgctaaaaa tgcggtattg ccgatcctgg cagctgcatt
     1801 gctcgcgtcg gatggtccca atatcatacg cgatgtgccg aatctttcgg acgtctccac
     1861 aatcaacgaa gtagtcaaga gcctgaatgc gaaggtcgaa tacgatccat ccagagggca
     1921 agtgatcatt gattcaacgg aagagctggc aagcgaagca catttcgatt atgtacgtaa
     1981 aatgcgcgcg tcaattttgg tgatggggcc attgctcgct cgcaacggtt ttgcacgcgt
     2041 agctttaccg ggcggatgcg caatcggatc cagaccgatc gaccagcacc ttaaagggtt
     2101 cgaagcgatg ggcgcggaaa tcacatttgg acatggacat gtcgaagcga aagcggaaaa
     2161 tggcctgaca ggcgctaaaa tctatttgga cttcccgagc gttggcgcta cggaaaatat
     2221 catgaccgcg gctgctcttg caaaagggac gacaattatc gaaaacgcgg cgaaagagcc
     2281 ggaaattgtc gatttagcga acttcatcaa tgaaatgggc ggaagagtgg tcggtgcggg
     2341 tacggatgca atccggatcg aaggcgtcga gaagcttcac ggaacaatcc accatatcat
     2401 cccggaccgt atcgaaacgg gcacattcat ggttgccgca gcaatcacgg gcggtgatgt
     2461 gacaatcgaa aacgctgttc ctgagcataa cgcagcgttg atttccaagc tcggcgaaat
     2521 gggcgtcatc atcacggaat tggacgaagg actgcgcatc cgggcgaagc atcctttgaa
     2581 agcggtcgat ctgaagacga tgccgcatcc tggattcccg acagacatgc aatcgcaaat
     2641 gatggcactc atgctgacgg catccggtac tggcgtactc acggaaacgg tattcgaaaa
     2701 ccgcttcatg cacgtcgagg aattccgtcg catgaacgca agtgttaaaa tcgaaggacg
     2761 ctcggtcatc atctcagggc catccaatct gcaaggtgca gaagtcgcag cgacagatct
     2821 tcgcgcggca gctgccctca tcctggcagg cctcgtagcg gaaggtgtca cacgcgtcac
     2881 ggaactggat cacctcgacc ggggctatgt cgatttccat aagaaattga aagcgctcgg
     2941 cgcagacatc gaacgcgttt caacggaaga aaagaaaaca gaaatagaga agcaactcgt
     3001 ttaaatcgta ctcatcctac accttattcg aagcttccat aactgctcct tgccctaacg
     3061 gcgaggggca gtttttttga atgaaacacg aggcttcatc atacaatcga atatggacaa
     3121 actactagcc gttttattca tcgtactact atttttcatt cccatcctta tgaaaccaac
     3181 agccgtacca aaggagaacg aagcaatgga agatccttgc gcaatcacaa tcactgtgga
     3241 aggggtggac aagcctatgc cgcttgagga gtacgtcgta ggggtcgtcg caggcgaaat
     3301 gccgatcgac ttccatgacg aagccttgag ggcacaagcg attgcggcta ggacatatgt
     3361 attgcgggcg acagatggcg gcaagaagcc gatcggggcg accgtctcag cccaagtgta
     3421 caaaaccgca gcggaacgga aagaacgctg ggggaagaag ttcaagcaga acgagaagaa
     3481 gctgcgtgga attgtcgcga cgaccgaagg ggatacaatc gtttatggtg atgaaatgat
     3541 cactgccatg tttttttcga cgtcgaacgg caagactgaa actgcaaaaa actacagcgg
     3601 cagccagatc ccgtacttgc agagcgtcga aagcccgggg gaagagctcg ttgcagccaa
     3661 agtggaacgg aagatcgaaa tgccgatctc ggagtggaat gaagcgctcg gatctgtttg
     3721 gaaagccgat aacttcaaaa gccttcagct cgtccgcaat caaacagggc gcgtgcaaaa
     3781 agcggtatcc accggcttcg aaatgagcgg ccgggacatg cgtgaagcgc tcggacttcc
     3841 ctccacggat ttcaacatcg cctacgacgt tacgaatcag atcgtcctga tcacgacgaa
     3901 gggatatggg cacggagtcg gcatgagcca atatggtgcc gaagccttcg cccgaaaagg
     3961 atggacagct gaaaacatac tgacctacta ttattccggc acaacaataa aaaagtttga
     4021 agtagacgaa tctgaatgtt taaaaacgcc aacacttgca aacaatagca agtgaggtga
     4081 tgataatgcg agaagaaaaa ccgaaatccc cttctcagaa gaataagagt cagaagaata
     4141 gatggttctg gccggccatc tacgcgggaa tcgcgattgt cttcgtcggc atgatttggg
     4201 gctacaatgc cttgatccaa aagggtccga acgaacaagc cgacgttgca acagatccga
     4261 acgccccgcc gctagtggaa acaaacgctt caaacgaagt actcaaattc ccgttcaacg
     4321 aagaccaact tgacagcatg gccattatac aagactacta tgatgtagag gctagtgaag
     4381 aagaccggga aaacgcacta ctcgtcttca atcaaacgta ttatacaaac aaaggcgtct
     4441 cgatctcgat ggaaggccaa ccatttgaag tcgtcgcagc catgagcgga acagttacag
     4501 aagtcgtaat cgatcaattc aaaggcgccg aagtcaaaat cgaacacgcc aacggattga
     4561 caacactgta cggctccttg accggcatcc tcgtcaaaga aggcgatgaa gtcgtccaag
     4621 gccaatcact cggcaccgcc acaagcaacg aatggaatgc caaagccgga acacacctgc
     4681 atttcgaagt gcacaaagac ggcaacccaa tcaacccgcg ctcaatgcta ggattctaat
     4741 gaaccaaacc actctctttg ggagagtggt tttttcatgt atcgcg
//
LOCUS       DASULI010000249         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1799642,
            whole genome shotgun sequence.
ACCESSION   DASULI010000249 DASULI010000000
VERSION     DASULI010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799642"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="VFX34_05450"
     CDS             <1..1556
                     /locus_tag="VFX34_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5564382.1"
                     /translation="LLLKEVAKKVPHALAGDIEMMARIGGDEFAILTANLQSQENVER
                     IAKTILHLFELPFEVKSYQLYLTTSIGISIYPESGEDSGTLMKNAGLGVYLAKKGGHH
                     TYQIYSPSANVETYKLFYLRNELQYALHNDQFLVYYQPIVQAGTDEIVTVEALIRWNH
                     PVWGIVRPDEFIPLAEESGLIIKIGEWVLKTVCRKLRAWHDAGYMVKGSVNLSLTQFL
                     QTDLIGVIERTLDENGLDPEWLILEITETAMWVQKDKALEKIAQLRALGITISLDDFG
                     AGYASFKNLLEIKPDTLKIDRSLVKNIPEDKDSAEVLTSLVQLAHRLSILVVVEGVET
                     EEQKEFVLGLEVDRMQGYLFSRPVSEENMQKLLERTAKPEIETAPGEERRKYFRIEFP
                     YPLEAMMTISELNGKKVQLGNTKILVENLGPGGLRFLSTIKLPVGSDMTLKFQTVIED
                     EEMIVYGKIIHESEFKELNRYGVEFHIDEEGREHLIKSFNQLQLQLRKNPLPPGCKFH
                     TENVSAYFE"
     gene            complement(1733..>2326)
                     /locus_tag="VFX34_05455"
     CDS             complement(1733..>2326)
                     /locus_tag="VFX34_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564383.1"
                     /translation="EDSYETASVQRDTHFTDFLLNKLKEEERVTGTPANQPVDKITSM
                     DLEIIVDDNTNPRHLIRFYHAPYTPLRKDSLGYAAHLKERQEWFTRLKTIIEQQNDAA
                     QKAESPIETFETQVHAETPKPVVTQEKSRLTVDVDMDRYALSLYKDAKDPSEANPEIK
                     QDAPKHEQVTEKPKSYFEQLLEKNRRQLRGDYSDEEN"
BASE COUNT          619 a          493 c          587 g          627 t
ORIGIN      
        1 atcttttatt gaaggaagtt gctaaaaaag taccccatgc acttgctggc gatatcgaaa
       61 tgatggcgag gatcggcgga gacgagttcg ccattttaac ggccaacctt caatcccagg
      121 agaatgtcga gcggatcgca aaaacaattt tacacctatt tgagctgccg tttgaagtga
      181 agagctatca gctatatctg acgacgagca tcgggatcag catttatccc gaatccggcg
      241 aagactcggg cacgcttatg aagaacgctg ggctcggggt ctacctcgcg aaaaaaggcg
      301 ggcatcatac gtatcagatc tattcccctt ctgccaatgt cgagacgtac aaattatttt
      361 atttgcggaa tgaactgcag tacgctttgc ataatgatca attccttgtt tattaccaac
      421 cgattgtaca agcggggacg gatgagattg tcacggtgga ggcgcttatc cggtggaatc
      481 atccagtgtg ggggatcgtc aggcccgacg aattcatccc gctggcagag gaatcggggt
      541 tgatcataaa gatcggcgag tgggtgttga aaacggtctg ccggaaattg cgagcgtggc
      601 atgatgccgg gtatatggtg aagggttccg tcaatctttc attgacccaa tttctgcaga
      661 cagatttgat cggcgtgatt gaaaggactt tagacgaaaa tgggctggat ccggaatggc
      721 tcatccttga aattacggaa actgcgatgt gggtgcaaaa agataaggcg ctggagaaaa
      781 tcgcccaatt acgtgcactt ggaataacca tatcgctcga cgatttcggt gcagggtatg
      841 cttcatttaa aaatctattg gaaatcaaac cagatacatt gaaaatcgat cgctccttag
      901 tgaaaaatat accagaggat aaggatagtg ctgaagtttt gacgtccctt gttcaacttg
      961 cccaccggct ttccatactc gtcgtcgtgg aaggggtcga aacggaagag cagaaagaat
     1021 ttgtattggg gctcgaagtc gataggatgc aagggtattt gttcagcaga ccggtttctg
     1081 aagaaaatat gcagaaatta ttggagagaa cggcaaaacc ggagattgaa acggcccccg
     1141 gggaagagcg caggaaatat ttcagaatcg aattcccata cccgctcgaa gcgatgatga
     1201 caatttcaga actgaacggg aaaaaagtgc agttagggaa cacgaaaatc ctggtggaaa
     1261 atctcggacc cggcggcttg cgtttcttat cgaccatcaa gctgcctgta ggttcggata
     1321 tgaccctaaa gttccaaacg gtgattgagg atgaagagat gatagtatac gggaaaatca
     1381 tccatgagtc cgagttcaaa gagctgaatc gatacggcgt cgaatttcat attgatgagg
     1441 aaggcaggga gcacctcatt aaaagcttca atcagctgca actccagtta cgaaaaaacc
     1501 ccctcccgcc tggctgtaaa tttcacactg agaatgtgtc tgcctatttt gagtaaaatt
     1561 cagaatagtg tcggtgcctg tgcagcgaac gattcagata atacactaca attgtatatg
     1621 gtaaaatgaa aagccaatca cctcaacggt ggattggctt ttgcacgttt atttattcat
     1681 caggttgaat tgtcataatt gcaccgtgca caggcaccgg cacaattcag gctcaattct
     1741 cttcgtcgga gtagtcgccg cgaagttgtc ttctgttttt ctctagtagt tgctcgaagt
     1801 aggattttgg tttttcggtg acttgttcat gtttcggagc gtcctgcttg atttctggat
     1861 ttgcttccga agggtccttc gcatccttat acaatgacaa tgcataccgg tccatgtcga
     1921 cgtccactgt aagccgcgat ttttcttgcg ttacaaccgg tttcggagtt tccgcatgaa
     1981 cttgagtctc gaacgtttca atcgggctct ctgccttctg tgcagcatca ttctgttgct
     2041 cgatgatggt tttcagccta gtaaaccatt cctggcgctc ttttaaatgc gccgcatagc
     2101 cgagtgaatc ttttcgcaac ggggtgtaag gagcatgata aaaacgaatg agatgtctcg
     2161 ggttcgtatt gtcgtcaacg atgatttcaa ggtccatcga cgtgattttg tcgacgggtt
     2221 gattggctgg agtccccgtc accctttctt cctcctttaa tttattcaat aggaagtcgg
     2281 tgaagtgtgt atctctttga accgatgcag tttcataact atcttc
//
LOCUS       DASULI010000250         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1599765,
            whole genome shotgun sequence.
ACCESSION   DASULI010000250 DASULI010000000
VERSION     DASULI010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599765"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFX34_05460"
     CDS             <1..883
                     /locus_tag="VFX34_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5564384.1"
                     /translation="LVFPFLKDQFANLVEEFPTYFNQMLINIDTFLRTSIFSSYYKEM
                     DININTLLDNGKGNIGELITDTVGGIASGITSFISALTGFILAIVTVPFILFYLLKDG
                     EKLPQMIKRMLPPRLRDDAEVILQDADHQISAYIQGQILVAISIGIMVSIGFRIIGME
                     YALLLGALAMFTSVVPYLGPLIAITPAVIIAIVTSPFMLVKLAAVWTIVQLIDGKFIS
                     PQIMGKSLKIHPITIIFVLLTAGSLFGVAGVVLGIPGYALLKVVFTHLFKLLKVRYNV
                     YEPDQAMHYEDSNEQMK"
     gene            complement(996..1985)
                     /locus_tag="VFX34_05465"
     CDS             complement(996..1985)
                     /locus_tag="VFX34_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020060758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5564385.1"
                     /translation="MTNHQAASYTTEEIADHLKVSKLTVYDLIKKGDLRAYRVGRQMR
                     IDAEDLEAYKDKTKSGGVIEKQAAAPSLKPEKPIQRTESPAPPRPYQSAPLQKLIISG
                     QDISLDLLANAIGRTGAVHNPLRSYTGSLNSLLAMYNGEADIVSTHLFDGDTGTYNLP
                     YINRILSGREYIVVNLLSRSAGLFVQKNNPKNIRTWKDFANPTIKMVNREIGSGARIL
                     LDEKLNLEGIVPRHINGYHSEEMNHIAVASKVASGIADVGVGIEKAASMVGVDFIPLI
                     HERYDLVMYKTAENEFLRESILSILRSPEFQNEIGALGGYDLSLTGTIMYETE"
BASE COUNT          591 a          471 c          501 g          712 t
ORIGIN      
        1 tctcgttttc ccatttttga aagaccagtt tgcaaacttg gttgaagagt ttccaacgta
       61 tttcaatcaa atgcttatca atatagatac atttttacgg acttcgattt tttcatccta
      121 ttataaggaa atggatatta acatcaacac ccttttagac aatggaaaag ggaatatcgg
      181 agaattgata accgatactg tcggcggaat cgcttccggc attacgtctt ttatttctgc
      241 gttgacgggt tttattttgg caattgtcac tgtgcctttc atccttttct atttattgaa
      301 ggatggagaa aagcttccgc aaatgattaa acgaatgttg ccgccacgcc tccgtgatga
      361 tgctgaagtg attctccagg atgcagacca tcagatcagc gcctatatac aagggcaaat
      421 ccttgtcgcc atcagcatag gaatcatggt gtcgatcgga ttccggatca tcggcatgga
      481 atatgcgctg ttattgggtg cgttagctat gtttacaagc gttgtgccct atttgggacc
      541 gctcattgca atcacgcctg cagtcatcat cgccatcgtc acttcgcctt tcatgctcgt
      601 gaaattggct gcagtctgga caatcgtgca gttgatagac ggaaaattca tatcgccgca
      661 aataatgggg aagtcgttga aaatccatcc gattacaatc attttcgtcc tattgactgc
      721 gggatctttg ttcggtgtcg caggggtcgt ccttgggata cccggatatg cattgttgaa
      781 agtggtcttc acacatttat ttaaattgct gaaggtcagg tacaatgttt acgaacctga
      841 tcaggcaatg cattatgaag atagtaatga acagatgaaa tgaacgtccg aattgggcgt
      901 tcatttttta gcttaaataa tattattatg taaactagtg atgaactaat aaagaaaaat
      961 gaaaaagggc acccatttct ctcagtgccc ttttcctatt ccgtttcgta catgattgtt
     1021 ccagtcaatg aaaggtcata tcctcctaac gcaccgattt cattttgaaa ttcaggtgag
     1081 cgcaagatgg ataggatcga ttcacgcaaa aattcattct ctgcagtttt atacatgaca
     1141 aggtcgtagc gctcatggat caaagggata aaatcgacgc ccaccattga tgcagccttt
     1201 tcgatgccaa ctccgacgtc cgcaattccg gatgccactt tgcttgcaac agcgatatga
     1261 ttcatttcct ctgaatgata accattgata tgccgtggga cgattccttc aagattcaat
     1321 ttctcatcga gcagaattcg tgcccctgat ccgatttccc gattgaccat tttgatagtc
     1381 ggattggcga aatctttcca tgtccggata ttcttcggat tgtttttttg gacgaacaag
     1441 cctgctgacc ttgacaataa gttgacgaca atgtattcac ggcccgaaag gattctgttg
     1501 atgtacggaa gattatacgt tcctgtgtct ccatcgaaca gatgcgtact cacgatgtct
     1561 gcttctccgt tatacatggc aagcagactg ttgagactgc cggtatatga acggagtggg
     1621 ttgtggacag cgccggttct cccaatcgca tttgccagca gatccaggct gatgtcctgt
     1681 ccactgatga tgagtttctg cagcggggcg gactggtatg gccttggggg agcgggggat
     1741 tcggttctct ggattggctt ttccggttta agcgaaggtg ctgcagcttg tttttcgatt
     1801 accccgccgc ttttcgtttt atctttatac gcttccaggt cctctgcgtc gatacgcatc
     1861 tgtcttccga cgcggtatgc gcggaggtct cccttcttaa tcaaatcata gacggtcagt
     1921 ttggagactt tcagatgatc cgctatttct tctgtcgtat atgaagctgc ctgatgattt
     1981 gtcataagtg tccctcctat acatttctac tattattttc acctataatg aaagtaaatg
     2041 caatctattc agatataacg aatgataact aatagtatga agttatattt aattataagt
     2101 agatatatca agttatatta tgttatatat aaatatagat agtattatcg ggttgaaagg
     2161 agagtcagat tgaaaaagtt tgttcaaggg atgcttctat tcgtcatgtt agccattcct
     2221 ctaggttgcg acacaaaaac gaatgaacgc gcgggaagga ctgagttgtc aattt
//
LOCUS       DASULI010000251         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1161742,
            whole genome shotgun sequence.
ACCESSION   DASULI010000251 DASULI010000000
VERSION     DASULI010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161742"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1383)
                     /locus_tag="VFX34_05470"
     CDS             complement(190..1383)
                     /locus_tag="VFX34_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX5564386.1"
                     /translation="MERVVGTVVRGLRCPIINKGDNIEEIVVDSVLKAAEVEGFAIND
                     RDIVTITESIVARAQDNYATVDHIAKDIQAKFGDSTIGVIFPILSRNRFAICLRGIAK
                     GAAKKIVLMLSYPSDEVGNHLVDIDTLDEKGVNPWTDVLTEEEFRGHFGYNKHTFTGI
                     DYIDYYKSIIEEYGVECEVIFSNNAKTILDYTKDVLTCDIHSRFRTKRILKANGGEKI
                     YSLDNILAESVDGSGFNEAYGLLGSNKATEDSVKLFPRNCQPVVDTIQASLEEKTGKT
                     VEVMVYGDGAFKDPVGKIWELADPVVSPAYTAGLDGTPNEIKLKYLADNNFADLRGEE
                     LKKAINEFIENKEEDLVGSMESQGTTPRKLTDLIGSLSDLTSGSGDKGTPIVFIQGYF
                     DNYTK"
     gene            1495..2376
                     /locus_tag="VFX34_05475"
     CDS             1495..2376
                     /locus_tag="VFX34_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5564387.1"
                     /translation="MVSKLDLYRIFCVVGKNESFSGAAKELFMTQPAISQSIMQLERE
                     LDTRLFNRTPKGVTLTNEGSLLLEYVNSAINLIEIGEEKIAEFKNLTTGELRIGVGDT
                     ISKYFLLPQLEAFRARYPNIKLKIENGTTDELISFLKSGEVDIVVCNLPVTDPTLNVM
                     PCMDIQDTFVYGDKYRKLLARPVGFHELAKLPLIFLEPSSNSRKYVEDHFLSKGVKIS
                     PEFELGSHDLVVEFARMNMGIACVTKEFTEDYLERGILHEVELTEQIPHRSIGVCSLK
                     SVPLSPASTRFLELLAG"
     gene            2575..3108
                     /locus_tag="VFX34_05480"
     CDS             2575..3108
                     /locus_tag="VFX34_05480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5564388.1"
                     /translation="MFTYKIDENTELRLLELRHAEQLFRLTDRSRESLRQWLPWLDFT
                     KSVADSRNFIGSTLQQFSRNDGFQAGIWYNGEIAGVIGLHGINWSNQSTTIGYWLGTG
                     FEGKGLMTKACKAVVDYCFRELELNRIEIRTATGNVKSAAIPQRLGFKQEGRLEQAEW
                     LYDKFVDHYVFGLVKEQ"
     gene            complement(3146..>3716)
                     /locus_tag="VFX34_05485"
     CDS             complement(3146..>3716)
                     /locus_tag="VFX34_05485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016717049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5564389.1"
                     /translation="GHAFREELKLMLSLMKPTYFVPIHGEYRMLQLHKEMAESIGVES
                     ENIFILNNGDVLDIQDGVARQTRNVEAGNVFVDGLGIGDVGRVVLRDRKLLSEEGMLI
                     IVVSMSKADGRIISDPDTISRGFVYERDAEDLYKEVNELVVSTIKEAKPSTRYRRNDL
                     KHAIRKAVEKLLYTRTKRRPMILPFIIEI"
BASE COUNT          944 a          845 c          884 g         1043 t
ORIGIN      
        1 atatatctcg ggaatgatcg ataacttctg ggaaatgaac gatatctctc cgaaaatgat
       61 cgatatccga cgggaaatgg tcaattaacc tcgggaaatg atcgatatcc ggaaaaccgc
      121 caccggcagc gcgtcatcca cctaaaatca acacgaaaaa gccctcagtc aatgactaag
      181 ggctgcatct tactttgtat agttatcaaa atatccttga atgaacacaa tcggagtgcc
      241 tttatcgccg ctgcctgaag tcaggtcgga taaagaaccg ataaggtcag tcagtttacg
      301 aggcgtcgtc ccttgggatt ccatggaacc aactagatct tcctctttgt tctcaatgaa
      361 ttcattgatt gcctttttca gctcttcgcc gcgcaggtca gcgaagttat tgtctgcaag
      421 gtatttcaat ttgatttcat tcggagttcc atcaaggcca gctgtgtagg caggggatac
      481 gactggatcc gcaagctccc agatttttcc gactgggtct ttgaacgctc cgtcgccata
      541 taccatgact tcgactgttt ttccggtttt ttcctccaaa gaagcttgga tcgtatcgac
      601 gaccggctgg caattgcgag ggaagagctt cacgctatct tcggtcgcct tgttggagcc
      661 aagcagtccg tatgcttcat taaaaccgct cccatcgaca gactcggcaa ggatattatc
      721 gaggctgtag atcttttcgc cgccgttcgc tttcaggatg cgtttcgttc tgaaacgtga
      781 atggatatca caagtgagca cgtctttcgt gtaatcaaga atcgtcttcg cgttattcga
      841 gaagatgact tcgcattcaa cgccgtactc ttcaatgatg gatttataat aatcgatgta
      901 atcgataccg gtaaatgtat gtttattgta gccaaaatgg ccacggaatt cttcttcagt
      961 caatacgtcc gtccatggat tgacgccttt ttcgtcgagc gtgtcgatat caacgagatg
     1021 gttgccgact tcatcggatg gatagctgag catcaaaacg attttcttcg ctgcaccttt
     1081 cgcaataccg cgaaggcaga tggcgaatcg attccggctt aaaatcggga aaatgacacc
     1141 gattgtgctg tcgccgaatt tcgcttggat gtctttcgca atatgatcga ctgtggcgta
     1201 attgtcttgg gcgcgagcta cgatggattc cgtgatggtg acgatatccc tgtcattgat
     1261 ggcaaatcct tcaacctctg ccgctttcaa tacgctatct acaacgattt cttcaatatt
     1321 gtccccttta ttgataatcg ggcaacgaag acctctgaca accgttccaa ctactctttc
     1381 caatgttatc gctccttatt gtataactta actgacgatc tacttgtact atactaccgt
     1441 ttaatggtat aagtaaaatt aatagttcta atgatgggta taaggggtgg ttatatggtt
     1501 agcaaattgg atttgtaccg tattttttgt gttgtcggca agaatgaaag cttttcaggg
     1561 gccgcaaagg agctattcat gacccagccc gccattagcc aatcaattat gcaactggaa
     1621 cgcgagctcg atacgcgcct gttcaatcgt acacccaagg gagtcacact gacgaatgaa
     1681 ggaagccttc ttttggagta tgttaattcg gccatcaatt taatcgaaat cggggaagaa
     1741 aagatagccg agttcaaaaa cctgacgacc ggcgaactgc ggatcggtgt cggggacacg
     1801 atttccaaat actttctcct tccccaactg gaagccttcc gtgctcgata cccgaatatc
     1861 aaattaaaaa tcgagaatgg cacgacggac gaacttattt cattcctgaa gtcgggggag
     1921 gtcgatatcg tcgtctgcaa tttgcctgtc acggatccca cattgaacgt gatgccttgc
     1981 atggatattc aggacacatt cgtctatggg gataaatatc gaaagctgct tgcgcgaccg
     2041 gtaggattcc atgaactcgc caagttgccg ctcatttttc ttgagccgag ttcgaattca
     2101 aggaaatatg tcgaggatca ttttttatct aagggcgtga aaatctcgcc cgagttcgaa
     2161 ttgggctccc atgatctcgt cgttgaattc gcgcggatga atatggggat tgcctgtgtg
     2221 acaaaggaat tcacggaaga ttatttggaa cgtgggattc ttcacgaagt cgagctgact
     2281 gagcagatac ctcatcgaag catcggcgtt tgctcgttga aaagtgtacc actttcgccg
     2341 gcatcgacga ggtttttgga gctactggca ggatagttat atggaagtat tgagtttgcc
     2401 attgacttat gcgcaattat tttcgtttat gcgcgtttag cgagatttat gagtatttat
     2461 ttccatttat gcgtttttgg aaagaattat gcgtatataa gtgggtttat gcgtgctttt
     2521 caggatttat gcgtttctgg cacggttgaa tcaaacaaag ggggagtcag tagaatgttt
     2581 acgtataaaa ttgatgaaaa tactgagctg cggttattgg aactgagaca tgctgagcag
     2641 ttattccgat tgaccgatcg atcacgggaa agccttcgcc aatggcttcc gtggctcgat
     2701 ttcacgaagt cggttgccga ttcaaggaac ttcatcggta gtacattgca gcagttcagc
     2761 cggaatgacg gcttccaggc aggcatctgg tacaacgggg agattgccgg cgtgatcggc
     2821 ttgcatggca ttaattggtc gaaccaatcg acgacgatcg gctattggct aggtaccgga
     2881 ttcgaaggca aaggcttgat gacgaaagca tgcaaggcgg ttgttgatta ttgtttccgg
     2941 gaattggagc tgaatcggat tgaaatccgt acggcgactg gaaatgtaaa aagcgcagcc
     3001 atcccgcaac gactcggctt caagcaggaa ggccgtttgg aacaagcaga gtggctgtat
     3061 gataagtttg ttgaccacta tgtgttcggc ttagtgaaag aacaataata aagcggagga
     3121 cgcccgatgt cctccgcttc attttttata tctcaataat aaaaggcaag atcataggcc
     3181 ttcttttcgt tcttgtataa agcagctttt ctaccgcttt ccggatggca tgtttcagat
     3241 cgttccgccg atagcgggtg gatggttttg cttccttgat cgttgagacg accagctcgt
     3301 tcacttcttt gtaaaggtcc tccgcatcgc gttcgtagac gaatccccgt gaaatcgtgt
     3361 ccggatccga aatgatccgg ccatccgctt tgctcatcga aacgacgatg atgagcatcc
     3421 cttcctctga aagcaatttg cgatcgcgca aaacgaccct tccaacatca ccgataccca
     3481 gcccgtccac aaagacattc ccggcctcca cattcctcgt ttggcgggcg actccgtcct
     3541 gaatgtccaa gacatcaccg ttattcagaa tgaagatatt ttcactttcc acaccgatcg
     3601 actctgccat ttctttatgc agctgcagca tgcggtattc accatggatg ggaacgaagt
     3661 acgtcggttt cattaatgaa agcatcagct tcaattcttc ccggaaagca tgtccg
//
LOCUS       DASULI010000252         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1887967,
            whole genome shotgun sequence.
ACCESSION   DASULI010000252 DASULI010000000
VERSION     DASULI010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887967"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFX34_05490"
     CDS             <1..339
                     /locus_tag="VFX34_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5564390.1"
                     /translation="YSGDDALTLPLLSIGGDGVISVASHLVGAEMQQMINAFKEGDTA
                     KAAWMHRTLLPLFHAVFSAPNPVPVKYGLAKIGIETGGVRMPLTAFGKDSAAFDEVWN
                     KFKKNEHVFQ"
     gene            583..2250
                     /locus_tag="VFX34_05495"
     CDS             583..2250
                     /locus_tag="VFX34_05495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5564391.1"
                     /translation="MAKTKNEIIKVIPLGGVGEIGKAMYVVEIDDELFIVDAGLMFPE
                     GEMLGIDIVIPDLTYIEENKDRVKGIFLTHGHEDAIGSIAYLLQKVQAPVYGSKLTLA
                     LAKEHLKEMPAPAGVKFFEVTNKSRMNFKNTYVTFFHTTHSIPDSLGIVFHTSEGAIV
                     HTGEFKFDQSAKGKYRPDIAKMAALGEEGVFILMSDSNEAERPGYTTSETVIEDRLSN
                     TFHSAEGRILVALYASNFIRIQQVFDKALESGKKVAVVGKSLESYFEVGMRLGYLQID
                     EETVVSVKDIGKYSDDQLVIIVTGNQGEPLDALEKIVRKHHKDIKIKDTDTVLITFTP
                     SPTMEVSMYQMANELSKAGANVLTASKNVHVSGHGSQEDLKLMLNLMQPKYFIPIQGE
                     YKMLIAHSKLAQQVGLQKSQIFIADKGDIVEYKNDKMRMSGRVQAGNILIDGIGVGDV
                     GNIVLRDRKLLSQDGIFTVVITLSRRQKRIAAGPEIVSRGFVYVRESEELLEESRNLV
                     RKIVEKYVNKDTFEWTNIKQEIRDTLSSYLFQQTKRRPMIIPIIMEY"
     gene            2362..>4156
                     /locus_tag="VFX34_05500"
     CDS             2362..>4156
                     /locus_tag="VFX34_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX5564392.1"
                     /translation="MSKKSKKKKRAAASRKKNDGLHPLVYEVAGLTMIGFAIIIIFEF
                     GIVGRGLSSFARLLFGNWHGAVPLLLIVQALLFMVKRKTVGWKNRIVGGSLFILASLL
                     LFSHIYLFKELYESKVLMSNSAFKETWKVLVTNEGIASRSGALGGGLAGAMLFALFHS
                     LFDSAGATVAGVLLLLIGIILLTGKALVPYIAELYPKLLASMKNKVDRKGKKASKKGN
                     QSAMSRTAMRSKQPAPIEDDKQQDMGGTAAAIEEQEEILSTPIISAFNERIEPIQSSD
                     GHVAVIEKETEAQPAIDSDTVDVPTYSGVSGEEENVSYVLPPPSLLRQTPSNDQSAEY
                     DSIHANAQKLERTFLSFGVKAKVTQVHLGPAVTKYEVLPDTGVKVSRIVSLADDIALA
                     LAASDIRIEAPIPGKSAVGIEVPNKAVAIVSLREVIDSKENNRPDSKLSIVLGRDVTG
                     QAMMAELNKMPHVLVAGSTGSGKSVCINGIIISILMRAKPHEVKMMMIDPKMVELNVY
                     NGVPHLLAPVVTDPRKASQALKKVVSEMERRYELFSHTGTRNIEGYNEHIEQWNEEND
                     EKHPRMPYIVVIVDELADLMMVASSDVEDSIT"
BASE COUNT         1261 a          866 c         1035 g          994 t
ORIGIN      
        1 tacagtggag acgatgcatt gacattgccg ttattatcga taggtggaga cggcgtcatt
       61 tctgtcgctt cccatcttgt cggggccgaa atgcagcaaa tgatcaatgc tttcaaagaa
      121 ggcgatacgg caaaggcagc ttggatgcac agaacattgc tgccattatt ccacgcagta
      181 ttttccgctc cgaatcccgt cccggtcaaa tacggcctgg ccaagatcgg tattgaaacg
      241 ggtggagtcc ggatgccgtt gacagctttc ggaaaagact ccgccgcctt cgacgaagta
      301 tggaataaat tcaaaaaaaa cgagcatgtt tttcaataaa ttaaagccgg tagcaatcac
      361 cggctttttt tgtttgtgta gtaggggcat tctcacgtat aatgaaactt agtcgttgtg
      421 gacgggtatt caaataacaa acaacaacat aatcaaatga aaatggacag ttggaattca
      481 ataagaaatt ataggcgaac acaatgaacg ccagtagagc atacatagac ggttcaaagt
      541 ccaacaccgg tctttttcaa acaaagtata ggaggaaaaa atgtggcaaa aacgaaaaac
      601 gaaataataa aagtgatacc gctaggaggt gtcggggaaa tcggaaaagc gatgtatgtc
      661 gttgaaatcg acgatgagct tttcatcgtt gacgcggggc tcatgttccc tgaaggcgaa
      721 atgctcggca ttgacatcgt cattccggac ctgacataca tcgaggaaaa taaagatcgg
      781 gtgaaaggga tcttcctgac gcacggtcat gaagatgcga tcggatccat cgcctacctg
      841 ctgcaaaaag tgcaggctcc cgtttacggt tccaaactga cgcttgcctt agcgaaggag
      901 catctgaaag agatgccggc tcctgcaggc gtgaaattct ttgaagtgac gaataaaagc
      961 cggatgaact tcaaaaacac ctatgtgact tttttccata cgacacatag catcccggat
     1021 tctctcggca tcgttttcca tacgagcgaa ggtgcaatcg tccatacagg cgaattcaag
     1081 ttcgaccaat ccgcaaaagg gaaataccgt ccggacatcg caaaaatggc ggctttgggc
     1141 gaagaaggcg tcttcatttt aatgtccgat tcaaatgaag ccgaacgtcc aggatatacg
     1201 acctccgaaa ctgtcataga agatcggttg tcaaatacat tccatagcgc ggaaggacga
     1261 attcttgttg ctttatatgc atccaatttc atccggatcc aacaggtttt cgataaagcg
     1321 cttgaatccg gaaagaaggt tgcggtcgtc ggcaagagcc tggaaagcta ttttgaagtt
     1381 ggcatgcgat taggctattt gcaaattgat gaagaaacag tcgtctccgt aaaagacatc
     1441 ggcaaatatt ccgatgatca acttgtcatc atcgttacag gcaatcaagg cgagccgctt
     1501 gatgcgcttg aaaagattgt ccggaaacac cataaggata ttaaaatcaa agatacggac
     1561 acagtcctta tcacattcac accatcgcct acgatggaag tttccatgta tcaaatggca
     1621 aatgagctat cgaaagccgg agcaaatgtc ctcactgcct caaagaatgt ccacgtatcc
     1681 ggtcacggca gccaggaaga tttgaagctc atgctgaatt tgatgcagcc gaagtatttc
     1741 attcctatcc aaggggaata taaaatgctg attgcgcatt cgaaacttgc ccaacaggtc
     1801 ggattgcaaa aatcccaaat cttcatagca gacaaagggg atatcgtcga atacaaaaat
     1861 gataaaatgc ggatgagcgg ccgagtacaa gccggaaata tcctcattga cggaatcggc
     1921 gtcggcgatg tcggcaatat cgtattgcgt gacaggaaac tgttgtcgca agatggcatt
     1981 tttacagtcg tcattacgtt aagccgcaga cagaaaagga ttgctgcagg accggaaatc
     2041 gtttcccgcg gtttcgtcta tgtaagggaa tccgaggagc ttctcgaaga gtcgaggaac
     2101 ctcgtacgga aaatcgtgga aaagtatgtg aataaagata cgtttgaatg gacaaacata
     2161 aagcaagaga tccgtgatac attaagctct tatttattcc aacagacaaa gagaagaccg
     2221 atgatcattc ctattatcat ggagtactga acgagcgtct acgcggaata aaacaacgac
     2281 acggaaaaac aacaaacttg agaaaaggat ttccatgccg accggacggc gggaaatcct
     2341 tttttcgaaa ggaggcgtga catgtccaaa aaatcgaaga aaaagaaaag ggcggccgca
     2401 tcaagaaaga agaatgacgg tctccatcca ttagtgtacg aagtggccgg tttgacgatg
     2461 atcggcttcg caatcatcat catttttgag ttcggcattg tcgggagagg cttatcgtct
     2521 tttgcaagac tgctattcgg gaattggcat ggggctgtac ctttgctgtt aatcgtccag
     2581 gcacttctgt tcatggtgaa acggaagacg gtaggttgga aaaaccgcat tgtcggcggc
     2641 agcctattca tcttggcaag tttgctatta ttcagccaca tttatttatt caaggagtta
     2701 tatgaaagta aagtgctcat gtccaattcg gcttttaaag aaacttggaa agtactcgtc
     2761 acaaacgaag ggatcgcttc aagaagtgga gctcttgggg gaggattggc aggagcaatg
     2821 ctttttgcac tcttccattc attatttgat tcggctggtg cgacagtggc gggtgtgctt
     2881 ctactattga tcggtattat tttgcttaca ggaaaggcgc tcgttccgta tattgcagag
     2941 ctttatccga aactgctcgc ctcaatgaaa aacaaagtgg atcgaaaagg aaagaaagca
     3001 tccaaaaaag gaaatcaatc tgccatgtca aggactgcga tgcgttcgaa acaaccggct
     3061 cctattgaag atgacaagca acaggatatg ggagggacgg cagctgcaat agaggaacag
     3121 gaagagattt tgtctactcc gatcatttct gcattcaatg aacgaatcga gccgatacaa
     3181 tcaagcgatg gacatgttgc ggtcatcgag aaggaaacgg aggcgcagcc ggctatcgat
     3241 tcggatacag tcgacgttcc gacctattcc ggcgtgtctg gcgaggagga aaatgtttca
     3301 tatgttttgc cgccgccatc attgttaagg cagacgcctt cgaatgatca atccgcagaa
     3361 tatgactcca ttcacgcgaa tgcccagaag cttgaaagga ctttcttaag cttcggcgtt
     3421 aaggcgaaag tgactcaagt ccatttagga cctgctgtaa cgaaatatga agtgctgccc
     3481 gatacggggg tcaaagtaag caggatcgtc agcttggccg atgatattgc gcttgccctc
     3541 gcagcaagtg atatacggat agaagcaccg attcctggaa agtcggcagt cggtattgaa
     3601 gtgccgaaca aagcggtagc catcgtcagc ttgcgggaag tgatcgattc aaaagagaat
     3661 aatcgcccgg attcgaagct gagcatcgtc cttggaagag atgtgacggg tcaggcaatg
     3721 atggccgaat tgaataaaat gccgcatgtc ctcgttgcag gttcgacggg tagcggaaaa
     3781 agtgtttgta tcaatggaat catcatcagt attttgatgc gcgccaaacc acatgaagtg
     3841 aagatgatga tgatcgaccc taaaatggtt gaattgaatg tctacaatgg tgttccacat
     3901 ctgttggcgc ctgtcgtcac cgatccgcgg aaagcatcac aagcactaaa aaaagtcgta
     3961 tccgaaatgg aaagaagata tgagctgttt tcgcatacag gcactcgtaa tattgaaggg
     4021 tacaacgaac atattgaaca gtggaatgaa gaaaatgacg aaaagcatcc gcgtatgcct
     4081 tatatcgtcg tcatcgtaga tgagcttgca gatttgatga tggttgcttc aagtgatgta
     4141 gaagactcca tcacac
//
LOCUS       DASULI010000253         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1790070,
            whole genome shotgun sequence.
ACCESSION   DASULI010000253 DASULI010000000
VERSION     DASULI010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790070"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFX34_05505"
     CDS             complement(<1..193)
                     /locus_tag="VFX34_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564393.1"
                     /translation="MESFADLDTFAWSGDWLPEEVVRDERGFVYTGREVPMDHWSDGR
                     PPAALETTVPGIFAAGDLRA"
     gene            619..1239
                     /locus_tag="VFX34_05510"
     CDS             619..1239
                     /locus_tag="VFX34_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEX5564394.1"
                     /translation="MFSPRKTDPFFAALLEIAEHVQEAMHYANDFKVTSVADLKEVSI
                     KLKEYETSGDDMIHDLISKLNISFMTPIEREDILQLAIKMDDILDGIEHFAANLEMFS
                     LTEIDEYVQKFMDNIVKSSDEIVKAMELLAKKKLVQMRDHAVLIKEYERICDEVLRTS
                     IKQLFIREKDPIRIIQFKDIYELLEDIADHCQDVANTIETIIMRNA"
     gene            1253..2251
                     /locus_tag="VFX34_05515"
     CDS             1253..2251
                     /locus_tag="VFX34_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX5564395.1"
                     /translation="MDTILVLTIAVVVFALAFDFINGFHDTANAIATSVSTRALKPRT
                     AVYMAAIMNFIGALTFTGVAKTISKDIVDPFLLDNGALVILAALTAAIAWNLITWYYG
                     IPSSSSHALIGSIAGAAISAAGFGILNYGGFLKIMQALIVSPFIAIAVGFLMMSLFKV
                     LLKNRNLFKANKRIRYLQIGTAALQSFTHGTNDAQKAMGIITMALIAAQMQTGDDIQL
                     WVRIAAATAMGLGTSIGGYKIIKTVGGKIMKIRPVNGAAADLSSAMIIFGATLIHLPV
                     STTHVISSAIMGVGSAQRVKGVKWGVAKKIVLTWVITLPISAALAAIVYQILNLFF"
     gene            2422..2622
                     /locus_tag="VFX34_05520"
     CDS             2422..2622
                     /locus_tag="VFX34_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX5564396.1"
                     /translation="MEQGKVKWFNAEKGYGFIEREDGDDVFVHFSAIQGDGFKTLEEG
                     QDVTFEIEQGQRGLQATNVEKN"
BASE COUNT          793 a          633 c          645 g          700 t
ORIGIN      
        1 ccgctctcag gtcaccggcg gcgaagatgc ccggcaccgt ggtctccagg gcggcgggcg
       61 gccggccgtc agaccagtgg tccatcggca cctcccggcc ggtgtagacg aaaccccgct
      121 cgtcgcgcac gacctcctcg ggcagccagt cgccgctcca agcgaaagta tcaagatcag
      181 caaatgattc cactctgtcg ttaactccaa aatatacccg actgcagatg taaatgaaat
      241 gaatgcaaga atgactgcgg gatccaaaag tgggctcgca tcgcctatcc cctctgcaat
      301 atcactgaag agaatataca gcacggttgc caatcccgcg atgataagca cggtcaaata
      361 gacattgata accggcattc cgaacacttc catcctctca cctcgccttc ttcaatttga
      421 attcaactat acatacgggc ggccgataat aaagtttcaa tattcccgga aaaatttttc
      481 ggactttcca acgtaaagat ttagcaaaac tgtcacttgc aaaaagaaaa aaggaaggat
      541 ttctgtactt atttttattg ttttttgcta tgataggact actaaaaatt gattccggaa
      601 acggatggag gcttaaaaat gttcagtcca cgtaaaaccg atcctttttt cgcagctctt
      661 ttggagattg ccgagcatgt tcaagaggcg atgcactatg cgaatgactt taaagtgaca
      721 tcagtagcag acttaaaaga agtgagcatc aagctgaagg aatacgaaac atcaggcgat
      781 gatatgatcc acgatctcat ttccaagctt aatatctcat tcatgacgcc gattgaacgg
      841 gaagacattt tacaattggc cattaaaatg gatgatattc tggatggcat cgaacatttc
      901 gcggcaaacc tagaaatgtt ctcgctcacg gaaattgatg aatacgtcca gaagttcatg
      961 gataatatcg tgaaaagctc cgatgaaatc gtcaaggcga tggagctatt agcgaagaag
     1021 aagcttgtcc aaatgcggga tcatgctgtt ttgatcaaag aatatgagcg catttgcgac
     1081 gaagtccttc gcacatcgat taaacagttg ttcatccgcg aaaaggatcc gattcgcatc
     1141 atccaattca aggatatcta tgagctgttg gaagacatcg cggatcattg ccaggatgtg
     1201 gccaacacaa tcgaaacaat catcatgcga aacgcgtaag gggtagctat tcatggatac
     1261 gatattagtt ctcacgatag cggtcgtggt ttttgcattg gctttcgatt tcatcaatgg
     1321 attccacgac accgcgaacg ccatagctac ttcggtttcg acgcgcgcat tgaagccgcg
     1381 cacagcggtc tacatggcag caatcatgaa tttcatcggc gcgttgacgt ttactggcgt
     1441 tgcaaagacg atttccaagg atatcgtcga tccgtttcta ttggataatg gagcgcttgt
     1501 catcttggcg gcccttacgg cggcaattgc ctggaatttg atcacgtggt attacggcat
     1561 tccgtcaagt tcatcacatg cgttgatcgg ttcgattgca ggtgctgcca tctcagcggc
     1621 aggattcggc attttaaatt acggcgggtt tcttaaaatc atgcaagccc tcatcgtgtc
     1681 accgttcatc gctattgcag tcggtttcct catgatgtcg ttgttcaagg tattattgaa
     1741 aaacaggaat ttatttaagg cgaataaacg gatccgctat ttacagatcg gaacagccgc
     1801 cctccaatcg ttcacacacg gtacgaacga cgctcaaaag gcaatgggta tcattacgat
     1861 ggcattgatt gcggcacaaa tgcagacagg cgatgatatc cagctatggg tccggattgc
     1921 cgctgcaaca gcgatgggcc ttggtacatc aatcggcggt tataagatca tcaagacggt
     1981 cggcggaaag atcatgaaaa tccgccctgt caacggcgct gctgccgatt tgagttctgc
     2041 aatgatcatt ttcggtgcaa cgctcatcca tttgcccgtc agcacgacgc atgtcatatc
     2101 ctccgctatc atgggggtcg gctcagccca acgtgtcaaa ggcgtaaaat ggggagtcgc
     2161 gaaaaaaatc gtcctcactt gggtgatcac attgccgatt tcagctgccc tagccgcaat
     2221 cgtgtatcaa attttaaact tgttcttcta ataaaaaact tttgaaaaac tttttacttg
     2281 cattgccaaa aaacctctgt tatgataaag aagaattatt tttgttcggc gtgatggtca
     2341 ctgtgtaata gcttgaccgc ttcaagactt gagcaaggat agtaacacaa tgtgtgcaca
     2401 gcacacaagg aggaaacaaa catggaacaa ggtaaagtaa aatggtttaa cgcagaaaaa
     2461 ggttatggct tcatcgaacg tgaagatggc gacgatgtat tcgtacactt ctccgctatc
     2521 caaggcgacg gcttcaagac tcttgaagaa ggccaagacg tgactttcga aatcgaacaa
     2581 ggtcaacgcg gacttcaagc tacaaacgtt gaaaaaaact aatttgaact aaccattcaa
     2641 aaccacttcc ttcggggagt ggtttttttc attgttgaag caaagctgac gtcggaatga
     2701 gaactctttg atgggaaatg agaactcttg atgccgggac gagaactctt cgacggagaa
     2761 tgagaactta a
//
LOCUS       DASULI010000254         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_702649, whole
            genome shotgun sequence.
ACCESSION   DASULI010000254 DASULI010000000
VERSION     DASULI010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702649"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFX34_05525"
     CDS             complement(<1..344)
                     /locus_tag="VFX34_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5564397.1"
                     /translation="MHLYGTQSVNNLGHLTIGGVDTVDLAHMYGTPLLVYDIALFKER
                     ATAFKETFAKAGIQAQVAYASKAFSSIAIYELAKKMGLSLDVVSGGELYTAAAADFPR
                     EKIHFHGNNKSYT"
     gene            complement(407..1855)
                     /locus_tag="VFX34_05530"
     CDS             complement(407..1855)
                     /locus_tag="VFX34_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HEX5564398.1"
                     /translation="MAKLFASLKDGEEWFHSKFGIDETFDATAKTINLWGMPVLMYYI
                     NGLIDGQTLTTLLVEMQEGFEKDDGHKDDPEWFLEYFPFYALEDVDDKEKFLTMILSG
                     LVGFVLPNGFVFAADLRSSPGRQPEEPDTEKVIRGARDGFTENIIVNTALIRRRLKTE
                     DLRFEMHQTTVNGKTDVAITYMKGAASEEHLDYIRKRLDDIKHDGLTMTDKSLEEFLF
                     KQRFHPMPFVRFTERPDICAASLLEGRIAIIVDTSPSVILVPMTIFDHLQHAEEYRQA
                     PFIGTFVRWVRLAGATLSLFLLPFWYLLSTYQQYLPISLSYIGPNDPGSIPLLVQLLI
                     ADIGIEVLRMAAIHTPSPMTTAMGLVAAIVIGQVAIDVGLFTGEVILYVAVSAIFTFA
                     IPSFELSITTKIFRIFILILTGILGPSGFFLGVVFLFYYLASLKPMGTPYLWPALPFF
                     PQAMQRVLIRLPMTIDAPRPFIADSPERNRLS"
     gene            complement(1859..2647)
                     /locus_tag="VFX34_05535"
     CDS             complement(1859..2647)
                     /locus_tag="VFX34_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigF/SigG family RNA polymerase sporulation
                     sigma factor"
                     /protein_id="HEX5564399.1"
                     /translation="MDTSVERQDALLSQEKMRLLIQQSQEGDKDARRMMVEGNTRLVW
                     SIVQRFASRGADLEDLFQIGCIGLMKSIDKFDLSYEVKFSTYAVPMIVGEIQRFLRDD
                     GMVKVSRSIRELSFKIRHATDDYIKNHGRSPSVSEIAEVLDVSIDEVILASDALRDPA
                     SLHEQLYENEGDSLTLMDQLRDDRSERFFDHIPLRDVVSKLNKRDQTIIYMRYYLDYT
                     QSDIAERIGISQVQVSRLEKKILAQLKNWMSAQPEESDNKVRTE"
     gene            complement(2651..3097)
                     /gene="spoIIAB"
                     /locus_tag="VFX34_05540"
     CDS             complement(2651..3097)
                     /gene="spoIIAB"
                     /locus_tag="VFX34_05540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HEX5564400.1"
                     /translation="MRNEMTLSFVAIEENEALARMAMTCFITPLDPTLEEISEFKTIV
                     SEAVTNAIIHGYESDGKSMVTIHAVIDENKVTMTVEDKGGGIFDVQQAMEPMFTTKPL
                     MERSGMGFTIMESFSDNLSVESSIGNGTVVRFEKMFSPVTEASRMR"
     gene            complement(3087..3437)
                     /locus_tag="VFX34_05545"
     CDS             complement(3087..3437)
                     /locus_tag="VFX34_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497186.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEX5564401.1"
                     /translation="MAEINQIDGIVVVTLAGELDNLEANRIRSTISSSIFTGKVKGIV
                     WDLSRLGFMDSAGIGLILGRMRDLAPFNGETLILNPSPTMEKIFNFSGLGSNIRHGSV
                     DSVIGEIGGVLHEK"
     gene            complement(3661..3822)
                     /locus_tag="VFX34_05550"
     CDS             complement(3661..3822)
                     /locus_tag="VFX34_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564402.1"
                     /translation="MIGAEVGDSCEHRYEERRLRVKAQRYEQEVKAALISAKNRRNTA
                     NRTLRCSIG"
BASE COUNT         1022 a          953 c          792 g         1097 t
ORIGIN      
        1 gtgtaacttt tattattccc gtgaaaatgg atcttttctc gcgggaagtc agcagctgcc
       61 gccgtgtaga gttctccccc tgaaacaaca tctagcgaga gtcccatttt tttagctagt
      121 tcataaatgg ctatagaaga aaaagctttg ctagcatatg ctacttgagc ctgtattccg
      181 gcttttgcaa aagtttcttt gaaggctgta gcgcgttctt tgaaaagagc aatatcatag
      241 acgagcaaag gtgtgccata catatgagca agatctactg tatctactcc gcctatcgtc
      301 aagtggccaa ggttattcac cgattgagtt ccatatagat gcatattcat tcctccaagt
      361 catgttgtta aaactttatc ataactggaa gccgggaatc aatcctctac gaaagcctat
      421 tccgttccgg agaatctgca atgaaaggtc ttggcgcatc gattgtcatt ggaagacgga
      481 tcaatacacg ttgcattgct tgtgggaaaa acggaagcgc cggccataaa tacggcgtgc
      541 ccatcggttt tagggatgct aaatagtaga aaaggaagac gacgcctaaa aagaatcccg
      601 aaggtcccaa aatccctgtc aaaatcagta tgaaaatcct gaatattttt gtcgtgatgc
      661 tcaattcaaa ggaagggatc gcaaatgtga agatggcgct tactgcaaca tacaaaatga
      721 cttctcctgt gaaaagacct acatctatcg ccacttggcc gatgacgatc gccgcgacga
      781 gtcccattgc cgtcgtcatc ggagatgggg tatgaatggc tgccatccgt aaaacttcta
      841 tgcctatatc cgcaattaat aactggacta aaagagggat ggatcccgga tcattcggtc
      901 caatatagct gagcgaaatt ggcaaatatt gctggtatgt cgaaagcaaa taccaaaatg
      961 ggagtaaaaa aaggctgagt gttgcacctg ccaaacggac ccaacggaca aacgtcccga
     1021 tgaatggtgc ttggcgatat tcttccgcat gctgcaagtg atcgaaaatc gtcataggga
     1081 ctaaaatgac ggaaggggat gtatcgacaa taatggcaat acgcccctcc aaaagagatg
     1141 cggcacaaat gtccggcctt tccgtgaatc ggacaaaggg cattgggtga aacctttgtt
     1201 tgaatagaaa ttcttccaac gacttatccg tcatcgttag accatcatgc ttgatgtcat
     1261 caagcctttt acgaatatag tcaaggtgct cctcgctggc agcacctttc atataagtga
     1321 tggcgacatc tgtttttcca ttcacagtcg tctggtgcat ttcaaagcga aggtcttccg
     1381 tcttcagcct ccttctaata agggctgtat ttacaatgat attttctgtg aatccatccc
     1441 ttgcaccccg aataaccttc tccgtgtcag gttcttccgg ttgtcttccc ggcgagcttc
     1501 ttaaatccgc cgcaaaaaca aatccgttgg gcaaaacaaa tccgacaaga ccacttaaaa
     1561 tcattgtaag gaatttctct ttgtcatcta catcttccaa agcataaaaa gggaaatatt
     1621 caagaaacca ttcggggtca tccttatgtc catcatcctt ttcaaacccc tcttgcatct
     1681 cgaccaataa agttgtcaac gtctgcccat cgataagacc gttaatataa tacatgagaa
     1741 ccggcatgcc ccataagttg atggtttttg cggtagcatc aaatgtttca tctataccga
     1801 atttgctgtg aaaccattct tccccatctt tcaaagatgc gaataatttt gccatgtgtt
     1861 attccgtcct cactttattg tcactttcct ctggttgtgc actcatccaa ttcttcaatt
     1921 gcgccaaaat cttcttttcc aaacgggata cttgcacttg tgatatgccg attcgttcag
     1981 cgatgtcact ttgtgtataa tccaagtaat agcgcatata tatgatggtc tgatcgcgtt
     2041 tattcagctt ggatacaacg tcccgaagcg gtatatgatc gaaaaacctt tccgatcggt
     2101 catccctcaa ctgatccatc aacgtcagac tatccccttc attttcgtac agctgttcat
     2161 gcaaggatgc cggatcgcgc aatgcatccg aagcaagaat gacttcatcg atcgagacat
     2221 ccaacacttc ggcaatctcc gaaacggatg gcgatctgcc atggttttta atatagtcat
     2281 ccgtcgcatg gcgaattttg aaactcagtt cacgaatcga cctgctcact ttcaccatgc
     2341 catcgtcccg aaggaagcgc tggatttccc cgacgatcat tggcacggca tacgttgaaa
     2401 acttcacttc ataagacaaa tcgaatttat caatcgattt catgagacct atgcagccga
     2461 tctgaaatag atcctccaag tccgctcccc gggatgcaaa tcgttgaaca atggaccaga
     2521 caagcctagt attcccctct accatcatcc ttctggcatc cttatcgcct tcttgggatt
     2581 gttggatgag cagtctcatt ttttcctgtg acaatagggc atcttgccgt tcaactgatg
     2641 tatccatcgg tcacctcatt ctgcttgctt ccgtgaccgg agaaaacatt ttctcgaatc
     2701 gcactaccgt tccatttccg atggacgatt ccaccgagag gttgtccgaa aagctttcca
     2761 tgattgtaaa tcccatacct gatcgttcca tgagcggttt tgtcgtgaac attggctcca
     2821 tcgcctgctg cacgtcaaaa atccctccgc ctttgtcttc cactgtcatc gtcactttgt
     2881 tttcgtcgat gactgcatgg attgtgacca tgcttttccc atcactttca tagccatgaa
     2941 tgatggcatt cgtcacagct tccgatacga tcgtcttgaa ttccgagatt tcttcaaggg
     3001 ttggatcgag cggtgttata aagcatgtca tcgccatcct cgccaacgct tcattttctt
     3061 cgattgcaac aaatgataat gtcatttcat ttctcatgta agacccctcc gatttcacca
     3121 attacactgt cgaccgagcc gtgtctgatg ttggaaccta agccggaaaa attgaagatt
     3181 ttctccattg tcggggatgg gttcaggatg agcgtttcac cgttaaacgg ggctaaatca
     3241 cgcatcctgc caagtatgag cccgatgcct gcgctatcca tgaatccgag gcggctcaaa
     3301 tcccagacga ttcctttcac ttttcctgtg aagatggaag atgagatcgt ggaacggatc
     3361 ctgtttgctt caagattgtc caactccccc gccaatgtta cgaccacaat gccatcaatt
     3421 tgattaattt cagccatgct gaatccctcc ttcttgcgga atcattcaac accgggcagg
     3481 gaaaatcctt tcacctgaca taactagtca tgaactttgt gtttttccta tctttcgaca
     3541 aagcattcta cctgaaaaag tctatccatt tttaggacgg tttacacatg ttcggaataa
     3601 atagagctat attataaagt tgatttacgc ttcgagcgga cgctttccgc gggcacggct
     3661 tcagccaatc gaacagcgaa gtgttcgatt tgccgtattt ctgcggtttt ttgcagaaat
     3721 taaggcagcc ttcacttcct gctcgtaacg ctgcgctttt actcgcaaac gccgttcttc
     3781 gtaacggtgt tcgcaggagt cgccgacctc cgctccaatc aacggtatct cctatcaaat
     3841 acttgaggga tagatctgat gagg
//
LOCUS       DASULI010000255         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1870375,
            whole genome shotgun sequence.
ACCESSION   DASULI010000255 DASULI010000000
VERSION     DASULI010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870375"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..125)
                     /gene="prli42"
                     /locus_tag="VFX34_05555"
     CDS             complement(27..125)
                     /gene="prli42"
                     /locus_tag="VFX34_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019154427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HEX5564403.1"
                     /translation="MSNARIRKIIVYIMIAAMVASTVMLGLSVLVG"
     gene            248..658
                     /gene="mce"
                     /locus_tag="VFX34_05560"
     CDS             248..658
                     /gene="mce"
                     /locus_tag="VFX34_05560"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX5564404.1"
                     /translation="MKKVDHIGIAVTNVDEAIHYYIHTHGLTHLGIEEVENQYVRVAF
                     IDAGNIKLELLEPIGGMGPIQTFLEKRGEGIHHIAFDVEGIRQRMEELRTAGVQLLGD
                     EPKTGAGGAEVMFLHPKSSFGVLYELCDKTGKGE"
     gene            665..2212
                     /locus_tag="VFX34_05565"
     CDS             665..2212
                     /locus_tag="VFX34_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5564405.1"
                     /translation="MDIYEKINELYDKKREIEMGGGDERIARQHERGKLTARERIDLL
                     VDPGSFVELNPFVVHRTRDFGMDKQAGPGDGVVTGYGKVDGRPVYLFSQDFTVFGGAL
                     GEMHAMKIANVMDLAAKNGAPFIGLNDSGGARIQEGVVSLDGYGQIFYRNSIYSGVIP
                     QISVIMGPCAGGAVYSPAITDFVFMVDQTSQMFITGPKVIETVTGEQISSEDLGGSKV
                     HNAISGNAHFRGETEEEVLKQVRQLLSYLPQNNEEKPPVLAYDKEDDYRPDLADVVPY
                     EATRPYDVRKVVEQVVDPNSFMEVQPEFARNVVVGFARIKGETVGLVCNQPRVMAGGL
                     DIDSSDKAARFIRFCDSFNIPIITFEDVTGFFPGIKQEHGGIIRHGAKILYAYSEATV
                     PKMTVILRKAYGGAYVALNSKSIGADVVYAWPNAEIAVMGPQGAANIIFAREIAQSED
                     PEATRAEKIEEYRAKFANPYVAASHGMVDDVIDPRETRIKLIQALEMMRNKKETRPAK
                     KHGNIPL"
     gene            2225..>3334
                     /locus_tag="VFX34_05570"
     CDS             2225..>3334
                     /locus_tag="VFX34_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5564406.1"
                     /translation="MNSQRLIDEFFELVQVDSETKNERNIANLLTKKMEELGFTVIED
                     DSAERSGHGAGNLIATMKGTVASADPIYFTCHMDTVVPGKGIKPILKEDGYIYSDGTT
                     ILGADDKAGIAALFEMIRTVQEKGIPHGDLQFVITAGEESGLAGAKAMDPSLIKAKYG
                     YAIDSDGKVGGIVTSAPYQAKLWTTIHGKTAHAGVAPEKGISAINIAAKAVAAMSLGR
                     IDSVTTANIGRFEGGQATNIVCDEVHILAEARSIVPEKLDRQTTHMSETFERVAREMG
                     GSATTEVKLMYPGFSFTESDEVVAIARKAVQNVGRTPQLLTSGGGSDANIFAGAGIPT
                     ATLSVGYEEIHTKNERMPVEELEKLTELLIEIVKVT"
BASE COUNT          984 a          672 c          876 g          802 t
ORIGIN      
        1 ggagtacatc cagtgttttt tgattctcag ccaactagca cacttaagcc taacattacg
       61 gtagacgcaa ccatggcagc aatcataata tacacgatta tttttcggat acgtgcgtta
      121 ctcattgtca aaaccccttc atttccatct atttttattg tagcagaact gtgttaattc
      181 tcaacaagta gcacaaatag ttgggaaaat gtacaatgga agaggataca taaaaggggt
      241 gggtgttatg aaaaaagtcg atcacatcgg tattgccgtt acgaacgtag atgaagcaat
      301 ccactattat atacatacac acggacttac acatctaggg attgaagagg tggaaaatca
      361 gtatgtccgt gtcgcattca tcgacgccgg gaatataaaa ttggagctac tcgagccaat
      421 tggtggcatg ggaccaatcc agacgtttct tgaaaaaaga ggggaaggca tccaccatat
      481 tgcatttgac gtggaaggaa ttcggcaacg aatggaggag cttcgaacag ccggcgtcca
      541 gcttttaggg gacgaaccga agactggggc aggaggagca gaggttatgt tcctgcatcc
      601 gaaatcctcg ttcggagtgc tgtatgaatt atgtgacaaa actggcaagg gagaatgaca
      661 cattatggac atctatgaaa aaattaatga attatatgat aaaaaacgtg aaattgaaat
      721 gggcggagga gatgaacgaa tcgcccgtca acatgagaga ggaaaactga cggcaagaga
      781 acggattgat ctgttggttg atccgggttc gtttgttgag ttgaatccgt ttgttgtcca
      841 ccgtacacgt gactttggta tggacaagca agcagggcca ggggatggcg tcgtaacagg
      901 gtacggaaaa gtcgatggcc gtccggtcta tctgttttct caggatttca ctgtttttgg
      961 tggggccctc ggtgagatgc acgccatgaa aatcgccaac gtcatggact tggcagcaaa
     1021 aaacggggca ccttttatcg ggctaaatga ttctggtggg gcccgaatcc aagagggcgt
     1081 tgtgtctctt gatggatatg gtcaaatttt ttatcggaac tccatctatt caggcgtgat
     1141 tccgcaaatc tctgtcatca tggggccttg cgcaggggga gcggtctatt ctccggcgat
     1201 tactgacttc gtgttcatgg tggatcagac gagccagatg tttattacgg ggccaaaagt
     1261 aattgaaaca gtcacaggtg agcaaatttc ctcggaagat cttggtggat caaaagtaca
     1321 taacgcaatc agtggaaatg cacacttcag aggcgaaact gaagaagagg ttttgaaaca
     1381 ggtccggcaa ttgctgtcgt atctacccca gaacaatgaa gaaaagcctc ctgttctagc
     1441 ctacgacaag gaagacgatt atcgtccaga tttggcggat gttgtgccgt acgaagcgac
     1501 ccgtccttat gacgtgcgga aagtcgttga gcaagtggtg gacccaaatt cctttatgga
     1561 agtccaacct gaattcgctc gcaatgtcgt cgtcggtttc gcacgcatca aaggagagac
     1621 ggtcggactt gtgtgcaatc agccaagagt gatggcaggc ggactcgata tcgactcttc
     1681 tgataaagcg gctcgtttca ttcgcttctg tgactcgttt aatataccga ttattacatt
     1741 tgaagatgta acgggtttct tcccaggcat taagcaggag catggcggca tcatccgtca
     1801 tggggcgaag attctatatg cgtattcaga agccactgtg ccaaaaatga cagttattct
     1861 ccggaaggca tatggtggag catatgtcgc actaaattcc aaatcaattg gagcggatgt
     1921 tgtctatgca tggccgaacg cagaaattgc agttatgggt cctcaaggag cggcaaacat
     1981 tatttttgcg cgggaaatag cacagtcgga agatccggaa gcaacacggg ctgaaaaaat
     2041 tgaagaatac cgtgcgaaat ttgcaaaccc ctatgtcgcc gcttctcatg gaatggtgga
     2101 tgacgtgatt gatccacggg aaacgcggat caagttaatc caagcactcg agatgatgcg
     2161 caataaaaaa gagacaaggc cagcaaaaaa gcatggaaac ataccgttgt aaggagtggg
     2221 cgaagtgaac agccaaagat taattgatga attttttgaa cttgtacaag ttgattccga
     2281 aacgaagaac gagcggaata tagcgaatct gttgactaag aagatggaag agctcggttt
     2341 tactgtgata gaagatgatt ccgcggaacg aagcggacat ggtgctggca atctgattgc
     2401 aacgatgaaa gggacagttg catctgcaga tccaatttat ttcacatgcc acatggatac
     2461 tgtcgtaccg ggaaagggaa tcaaacctat tctaaaagaa gacggctata tttattcgga
     2521 tgggacaacc attcttggcg cggatgataa agcagggatt gcagctttat tcgaaatgat
     2581 acgtaccgtg caagagaagg gaatccctca cggagatcta cagtttgtca ttactgcagg
     2641 cgaagaaagc ggtcttgcgg gcgcgaaagc gatggatcct tcactgatca aagcgaaata
     2701 tggctatgca attgacagtg acggcaaagt cggcggaatt gtcacgtctg ccccgtacca
     2761 agccaaacta tggacaacga ttcatgggaa aacagctcac gcgggagtcg ctcctgaaaa
     2821 agggatatct gccatcaata tcgcggcaaa agcggttgca gcaatgtccc tcggaagaat
     2881 tgattccgtt acaacagcga atatcggccg gttcgaaggc ggacaggcga caaacattgt
     2941 gtgcgatgaa gttcatattc ttgctgaagc acgatccatc gttcccgaga aattggatcg
     3001 ccagacgact catatgtcag aaaccttcga acgtgtcgct cgggaaatgg gaggatctgc
     3061 gacaacggaa gttaagttga tgtacccagg attctcattt actgaatcag atgaagtcgt
     3121 tgcgattgca aggaaagctg ttcaaaatgt tggtcgtaca ccgcaacttc ttacaagcgg
     3181 tggaggtagc gatgcgaata tttttgctgg tgccggaatc ccgacagcta cactttccgt
     3241 cggatacgaa gaaattcata caaaaaatga gcggatgcca gtagaagagc tcgagaagct
     3301 cactgaatta ctcatagaaa ttgtgaaagt aact
//
LOCUS       DASULI010000256         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_255853, whole
            genome shotgun sequence.
ACCESSION   DASULI010000256 DASULI010000000
VERSION     DASULI010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255853"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFX34_05575"
     CDS             complement(<1..836)
                     /locus_tag="VFX34_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5564407.1"
                     /translation="MNEQLFEKLDVAYEEMVVIRRHLHMHPELSFQEEKTAQFIHDFY
                     VDLGVDVRSGVGGNGVVARVKGARPGKTVALRADFDALPIQDEKDVPYKSTVPGVMHA
                     CGHDGHTAALLQLAKAIHELREDLAGEYVFIHQHAEEFAPGGAISMIEDGCLDGVDVI
                     FGTHLWSLIPYKTIEYLQGPVMAAADRFEITIQGRGGHGAAPHQTKDAIVIGAQLVMN
                     LQQLVSRRVDPIESAVLSVGSFIADNAFNVIADSAKLGGTVRTFNPDVRDMMEREMAR
                     VV"
     gene            complement(915..1430)
                     /locus_tag="VFX34_05580"
     CDS             complement(915..1430)
                     /locus_tag="VFX34_05580"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5564408.1"
                     /translation="MYMYLTTGTRDFMEKLKERHADKGMILMHGGGQSLLLHETEGKS
                     VFQTPRRYEVISSIGEMQEEGYYVFNNVPVTDEGKPVFEHRFSADSGRIESQKGFTAF
                     RLLRPLDSDTYIVMTEWEDASDYDRWKHSQAFKESPQSVDMNAFAGHTTHIFSSASYI
                     TTYVAKIDDKA"
     gene            1648..>2507
                     /gene="hemE"
                     /locus_tag="VFX34_05585"
     CDS             1648..>2507
                     /gene="hemE"
                     /locus_tag="VFX34_05585"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004230751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5564409.1"
                     /translation="MTTFNDTLLRAARGEKIDHTPVWFMRQAGRSQPEYRKIKEKYSL
                     EEITHQPELCAYVTKLPVDQYNVDAAILYKDIVTPLPGIGVDVKIKAGVGPVISNPIR
                     SVQDVHNLGELSPEDDIPFVIDTIKILKEQLNVPLIGFAGAPFTLASYMIEGGPSKSY
                     NLTKSFMVSEPEAWFALMNKLGDTIIASIKAQVAAGAAAIQVFDSWVGALNVSDYRVF
                     IKPVMTRIFNELRELNVPLITFGVGASHLANEWHDLPVDVVGLDWRLSVKEAGERGLT
                     KTLQGNLDPA"
BASE COUNT          635 a          632 c          633 g          607 t
ORIGIN      
        1 tccacgaccc gcgccatttc ccgttccatc atgtcgcgga cgtcaggatt gaatgtacgc
       61 accgtgccgc cgagctttgc cgaatcggcg atgacgttga atgcgttgtc cgcgatgaat
      121 gaaccgacgg ataaaacagc cgactcgatc ggatcgacgc ggcgggacac gagctgctga
      181 aggttcatga cgagctgcgc accaatgacg atggcatcct tcgtctggtg cggcgccgct
      241 ccatggccgc ctctgccttg gattgtaatt tcaaaacggt ctgcagccgc catgaccggc
      301 ccttgtaaat attcgatagt tttgtagggg atgagcgacc aaagatgcgt gccgaaaatg
      361 acatcgacac cgtcgaggca gccgtcctcg atcattgaaa tagccccgcc cggcgcaaat
      421 tcctccgcgt gctggtgaat gaagacatat tcccctgcga ggtcttcccg cagctcatgg
      481 atcgctttcg ccaactgcag caatgccgct gtatggccat cgtgtccgca ggcgtgcatg
      541 acgcccggca ctgtcgattt gtaagggaca tccttttcgt cctggatcgg caatgcgtcg
      601 aaatccgcgc gcaaagcgac cgtcttgccg ggacgggccc ctttgacgcg ggcgactaca
      661 ccgttgccgc cgacgcctga acgaacatca acacccagat ctacataaaa gtcatggatg
      721 aattgcgcgg ttttctcttc ttggaatgat aattccgggt gcatatgcag atgtctgcgg
      781 atgacgacca tttcttcata tgcgacatcc aatttctcga acagttgctc attcattcaa
      841 atacccctct cgaaacgcat tatttatatt cacaattata acaatacgtt ccgggggccg
      901 caatgcggtc taaattatgc cttgtcatcg attttcgcga catacgtcgt aatataggat
      961 gcgctcgaaa aaatatgcgt cgtgtggcct gcaaatgcat tcatgtcgac cgattgaggt
     1021 gattccttga atgcctgcga gtgtttccaa cggtcatagt cggaagcatc ctcccattcg
     1081 gtcatgacga tatacgtgtc cgaatcaagg ggacggagca gccggaaagc ggtgaaacct
     1141 ttctggcttt caatccggcc gctgtctgcg gaaaaacgat gttcgaagac aggcttgcct
     1201 tcatcggtga cggggacgtt attgaagacg tagtagccct cttcctgcat ttcaccgatt
     1261 gaactgatca cttcataccg cctcggtgtc tggaagacgg acttcccctc ggtttcatgc
     1321 aaaaggagcg actggccgcc gccatgcatg agaatcatcc ctttatccgc atggcgctcc
     1381 ttaagtttct ccatgaaatc ccttgttccg gtagtcaaat acatatacat ttcccaaaac
     1441 ctccttaatg aatgagtgct acctatattt tcccttaacg gggcaattaa aacaaacatt
     1501 gtcgaaatga tggcggcgag cggaaaatat gagtacaaat tgatgacaaa tgtggccaat
     1561 cactatacat tcaataagga aacgtctata tttgaaagcg aatatgtgaa cttcgataat
     1621 tattataaag aaagtgggaa tcgatttatg acaacattta acgatacact gcttcgtgct
     1681 gcaagaggag aaaaaatcga ccatacgccg gtttggttca tgagacaggc aggacgctcc
     1741 cagcccgagt accgcaagat caaggagaag tattcacttg aagaaattac acatcagccg
     1801 gagctttgcg cttatgtaac gaagcttccc gtcgaccaat acaacgtcga cgcggcgatt
     1861 ttgtataagg atatcgtaac gccgcttccg ggaatcggag tggacgtgaa aataaaagcg
     1921 ggtgtaggcc cggtcatttc aaacccgatc cgttcggtgc aggacgtgca taatcttggc
     1981 gagctttcgc cggaggacga tattcctttc gtcatcgata cgatcaaaat cttgaaagag
     2041 caattgaatg taccgttgat cggcttcgca ggagcgccat tcacactcgc gagctacatg
     2101 atcgagggcg gtccatcgaa aagctataac ttgacgaaat cgttcatggt gtctgagcct
     2161 gaagcgtggt ttgcgcttat gaataaattg ggtgatacga tcatcgcatc gatcaaagca
     2221 caagtggcag cgggagcagc cgccatccaa gttttcgatt catgggttgg cgcattgaat
     2281 gtctccgatt acagagtatt catcaagcct gtcatgacac gcatcttcaa tgagcttcgc
     2341 gaattgaatg tgccattgat cacgttcggc gtcggagcga gccatctggc gaatgaatgg
     2401 catgaccttc cagtcgacgt cgtcggcctg gactggcgtt tatcagtcaa ggaagccggg
     2461 gaaagaggat tgacgaagac attgcaaggg aacttggatc ctgcatt
//
LOCUS       DASULI010000257         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_922986, whole
            genome shotgun sequence.
ACCESSION   DASULI010000257 DASULI010000000
VERSION     DASULI010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922986"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VFX34_05590"
     CDS             complement(<1..1119)
                     /locus_tag="VFX34_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved virulence factor C family protein"
                     /protein_id="HEX5564410.1"
                     /translation="MKIVTIEPTPSPNTMKIVLDQELPFGTSHNYKKEDAATAPYPIS
                     DLLQIDGVKGIYHVADFLAVERNARFDWETILAAVNSIFGQEGETKSGEQQADDHYGE
                     VYVHVQMFKGIPLQVKVFDQQAEERFGLSERFINAMNDMQGDDEENYILQRKWADYGI
                     RYGEKAEIGAQVVQEIEAAYPQERLDDMIRQSKEGRTDTMQVGRKVTLEEFQTDDWEK
                     RFQLLDQMADPDMSDVPLLNQALEDEKMSIRRLATVYLGMIENEDVIPFIEKALQDKS
                     AAVRRTAADCMSDLGFEKFEDAAIRALQDKSKLVRWRAAMFLFESGTENALPALKEAQ
                     EDPEFEVKLQIRMAIARIEEGEEAKGSVWKQMTEARMAS"
     gene            complement(1147..>2420)
                     /locus_tag="VFX34_05595"
     CDS             complement(1147..>2420)
                     /locus_tag="VFX34_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5564411.1"
                     /translation="DVTGAVIFVTHDRYFLDHVATVIYELADKRLYSHTGNYGDYIEA
                     KAQRLEMEAATDAKLRNTYRSELKWIRRGAKARTTKQKARIERFEQLSDQISKDKPIG
                     ELDVSMATSRLGKKVLEGKSVSKRFDSNIILSNFSFLLKQGDRIAITGPNGVGKSTLM
                     GILAGELEPDSGEIDKGATVRIAHFTQHLPEMNPDQRMIEHIREISNDIEDADGVRVS
                     ATQMLERFLFPTSTHGTPIGKLSGGERKRLHLLRLLMEQPNVLLLDEPTNDLDLETLS
                     VLEQFIDTFPGVVVTISHDRFFLDRTSRELWILDGTGEVQKWQGIYSDYLAEEALKKE
                     EVTAPRQEPIREKREQKKKMTLAEKKEWETIESQIESVENQIMDVEEALQQAGADYEK
                     VRLHTEELERLNEQYEQLVERWSYLQEIAEQ"
BASE COUNT          566 a          632 c          478 g          744 t
ORIGIN      
        1 acttgccatc cgagcttctg tcatctgttt ccacacagag ccttttgctt cttcgccctc
       61 ttcaatacgg gcaatcgcca tccggatttg gagcttcact tcaaattccg ggtcctcttg
      121 cgcttctttc aatgccggca atgcgttttc tgttccagat tcaaataaaa acatggccgc
      181 acgccaacga acgagcttgc ttttatcttg taatgctcga attgcagcat cttcaaactt
      241 ttcaaatccg agatcactca tacagtctgc agctgtccga cgcactgccg cacttttatc
      301 ttgcaatgct ttttcgatga acggaatgac gtcttcgttc tcaatcatcc caagataaac
      361 agttgcgagg cgacggattg acattttttc atcttccaat gcttgattta agagggggac
      421 atcggacata tccgggtccg ccatctgatc aagtaattgg aatcgctttt cccaatcgtc
      481 cgtttggaat tcctcaagtg ttaccttacg gccaacttgc atcgtatcgg tacggccttc
      541 cttggattgg cgaatcatat catccaatcg ctcttgtgga tacgctgctt ctatttcttg
      601 gacgacttga gcaccaatct ccgccttttc accataacgg attccgtaat cggcccactt
      661 tcgctggagg atatagtttt cttcgtcatc accttgcata tcattcatcg cattgataaa
      721 tctctcacta agaccgaaac gttcttctgc ttgttggtca aacactttga cttgtagggg
      781 gatgcctttg aacatttgga catggacgta gacttcacca taatgatcgt ccgcttgctg
      841 ttcgccggat ttcgtttctc cttcttgacc aaaaatggaa ttgactgccg ccaatatcgt
      901 ttcccaatca aacctggcat tccgttcaac cgcaaggaaa tcagctacat ggtaaatacc
      961 tttaaccccg tcaatttgaa gtaggtctga aattggataa ggggcagttg ctgcatcttc
     1021 ttttttataa ttgtgactcg ttccaaaagg aagttcttga tcgagtacaa ttttcatcgt
     1081 attggggctt ggtgtcggtt caattgttac aattttcaaa gatatctcct cctgttaccc
     1141 attgtatcac tgctcggcga tttcctgcag atacgaccat ctttccacga gctgttcata
     1201 ttgttcattc aaacgctcga gctcttccgt gtgcaagcga actttttcgt aatcagctcc
     1261 cgcttgctgc aatgcttctt cgacgtccat gatttgattc tcgacagatt cgatctgaga
     1321 ctcaatcgtt tcccattcct tcttttcagc aagcgtcatc tttttctttt gctcacgttt
     1381 ctctctaata ggctcctgcc tcggagcggt aacttcctct tttttcagag cctcttccgc
     1441 taggtaatcg gaatagatgc cttgccactt ttgaacttct ccagttccat ctaatatcca
     1501 taattcacgt gaagtacggt caaggaagaa acggtcatga gaaatcgtca caacaacacc
     1561 tgggaatgtg tcgataaatt gctctaagac agaaagcgtt tcaaggtcca ggtcatttgt
     1621 cggctcgtcc aacaacaaaa cgtttggctg ttccatgagc aggcgaagta aatgcagacg
     1681 tttcctctcc ccgcccgaca gtttgccaat gggtgtacca tgagtggagg tcgggaataa
     1741 aaaccgttcc aacatttgtg tagccgaaac tcgaacacca tccgcatctt caatatcgtt
     1801 cgagatttcc cgtatgtgct caatcattcg ttggtctgga ttcatttccg gaagatgctg
     1861 cgtaaagtgt gcgatgcgga ccgtagcccc tttatcgatt tcaccggagt ccggttccag
     1921 ttcacctgca aggatgccca tcaaggtcga ctttccaact ccgttaggac ctgtaatcgc
     1981 aattcgatcc ccttgcttta acaggaagga gaaattcgaa aggatgatat ttgaatcaaa
     2041 acgcttcgac acactcttgc cttccagcac ctttttccca agccggctcg ttgccatcga
     2101 gacatcaagt tcacctatag gtttatcctt gctaatttgg tctgataatt gttcaaaccg
     2161 ttcaatgcga gccttttgct ttgtcgtcct cgctttcgcc cctcgtcgaa tccactttag
     2221 ctccgaacga tatgtattgc gcagcttcgc atctgttgcc gcttccattt caaggcgctg
     2281 tgccttcgct tcaatataat ctccgtaatt cccggtgtga gaatacagcc tcttatcagc
     2341 taattcgtaa atgaccgttg cgacatgatc taggaaataa cgatcatgcg tcacaaaaat
     2401 aacggcacct gtcacatctg
//
LOCUS       DASULI010000258         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_391553, whole
            genome shotgun sequence.
ACCESSION   DASULI010000258 DASULI010000000
VERSION     DASULI010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391553"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFX34_05600"
     CDS             <1..324
                     /locus_tag="VFX34_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5564412.1"
                     /translation="YDCSGFTYNMLKYAGYFIPRDASDQAKGGEEVPLEQPDEWKKGD
                     LLFFANDEGKGNVRHVGFYYGNGLMLHSPSTGKNIEVLELAGTKLERELCAVRRYGRM
                     GETTR"
     gene            321..1337
                     /locus_tag="VFX34_05605"
     CDS             321..1337
                     /locus_tag="VFX34_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019378187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5564413.1"
                     /translation="MTEQVLLSVQNLKRYFDIGRGEKLKAVDGISFDIYKGETFGLVG
                     ESGCGKSTAGRTILGLYNKTDGDVLFEGADVHTMNEKARKEFLKKMQMIFQDPYASLN
                     PRSTVFEIIAEPMEVHNLYTSRAKMRERVYELLEDVGLNRDHANRYPHEFSGGQRQRI
                     GIARALALDPDFIIADEPISALDVSVQAQVVKLLQRLQKEKGLTYLFIAHDLSMVKYI
                     SDRIGVMYLGHMVELTTSDKLYENPLHPYTEALLSAIPIPDPDIEEERERILLQGELP
                     SPINPPSGCVFRTRCPYAMEVCAELKPAWLEKEEGHFVACHLHDEAVMKRTDAVPVRE
                     AELA"
     gene            1453..1926
                     /locus_tag="VFX34_05610"
     CDS             1453..1926
                     /locus_tag="VFX34_05610"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5564414.1"
                     /translation="MMKRPMSQSRTVQTKLVLPPDTNHHGTIFGGRVLAYIDEIAAIA
                     AMKHSHAAVVTASIDSVDFLSSARVGDVLELEAIVCSTGRSSMEVFVTVHSENLLTGE
                     SKLTTESFLTMVALDESNIPTPVPGVFPETEEETALYETGPARRAHRKQRRELHH"
     gene            2022..>2203
                     /locus_tag="VFX34_05615"
     CDS             2022..>2203
                     /locus_tag="VFX34_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564415.1"
                     /translation="MKHDILERQRDLATRKNECDRLQRRYTAKLAELKEAERLRDQLQ
                     RQLSKEQQDVVKLGKFS"
BASE COUNT          610 a          457 c          602 g          534 t
ORIGIN      
        1 tatgattgct cgggcttcac gtataacatg ctgaagtatg cggggtactt tattccacgc
       61 gacgcgagtg accaggcgaa gggtggggaa gaagttccgc tcgagcaacc ggatgaatgg
      121 aagaaggggg atcttctctt ctttgcgaat gacgaaggga aaggcaacgt ccggcatgtt
      181 gggttttatt atggaaatgg cctcatgctc cactcaccat caaccgggaa gaacattgag
      241 gttttggaat tggcaggaac gaaattagaa cgcgagcttt gcgctgttcg gcgctacggc
      301 aggatggggg agacaacacg atgacagaac aagttctatt gtccgttcag aatttgaagc
      361 gatattttga tatcggccgt ggggagaagt taaaagctgt tgatgggatc agttttgata
      421 tttataaagg ggaaacgttt ggtcttgttg gtgagtccgg ttgtggtaaa tcaactgcgg
      481 ggcggacaat cttaggtctc tataataaaa ccgatggcga cgtcctgttc gaaggtgcgg
      541 atgtccatac gatgaacgag aaggcgagaa aggaattcct gaagaagatg cagatgatct
      601 tccaggatcc gtatgcttcg ctcaatccac gttcgacagt gtttgaaatt attgcagagc
      661 cgatggaagt gcataatctg tatacatcga gagcgaaaat gagagagcga gtctatgaac
      721 ttttagagga cgttgggctc aatcgggatc atgcgaaccg ctatccgcat gagttctccg
      781 gaggacagcg ccaacggatc gggattgcac gggctctcgc actagacccg gactttatta
      841 tcgcagatga accgatttcc gcactggatg tatcggtgca agcccaagtg gtgaagctcc
      901 tgcaacgact tcaaaaggaa aaggggctga cgtatttatt catcgcgcat gacttgtcga
      961 tggttaaata catctccgac cgaatcggtg tcatgtattt ggggcatatg gtcgaattaa
     1021 caacgagtga taagctctac gaaaatcctc ttcatccgta tacggaagcg ttactgtctg
     1081 caattccgat tccagacccg gatattgagg aggagcggga acggatttta ttgcaaggcg
     1141 aactgccaag cccgatcaat ccaccgtccg gatgcgtttt ccgtacacga tgcccgtatg
     1201 ccatggaagt ttgcgccgaa ttaaaaccgg cgtggcttga aaaagaagaa ggccattttg
     1261 tcgcctgtca tctacatgac gaagcggtga tgaagcgaac agatgcggtt cctgttcgag
     1321 aagcggagct tgcataatat attgtctgac tgtcccatag atggggcagt ttttcttttg
     1381 gcgggaatcc atgcgatgac gaggaaaagg ggtatactag gacagacact acgatataga
     1441 ggagtaatcg gtatgatgaa aagaccaatg agtcaatcca gaacagttca aacgaagctc
     1501 gttcttccac cagatacaaa tcaccacggg acaatcttcg ggggaagagt cctcgcgtat
     1561 atagatgaga ttgccgcgat tgcagcaatg aagcattccc acgcggctgt cgtaacggcg
     1621 tccattgact ccgtcgactt tctttcatcc gctcgggtcg gcgatgtctt agagttggag
     1681 gcaatcgtct gctcaacagg ccgttcgtcg atggaagtgt ttgttaccgt acattcggaa
     1741 aatctactta ctggcgaatc caagctaacg acagaatctt tcttaacaat ggtcgcgttg
     1801 gatgaatcga atataccgac accggtgcca ggcgttttcc ctgaaaccga agaggaaaca
     1861 gcattatacg aaacaggacc agcacgccgg gcacaccgga agcagcgcag agaattgcat
     1921 cattgatttt caaggaagac tactgtaaaa ggtagtcttt ttatcttttt atttgaaacc
     1981 tcctagaaat tccatcgtag aacagatagg gggtgtttgg gatgaaacac gatattcttg
     2041 aacgacaacg tgacttggca acaagaaaga atgagtgtga tcgattgcag cggcgctata
     2101 cagcgaaatt agctgaattg aaggaagcgg agcggcttcg tgaccaattg caacggcaat
     2161 tatcgaagga acagcaagat gttgtgaagt tgggcaaatt ttc
//
LOCUS       DASULI010000259         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1944050,
            whole genome shotgun sequence.
ACCESSION   DASULI010000259 DASULI010000000
VERSION     DASULI010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944050"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1660)
                     /locus_tag="VFX34_05620"
     CDS             complement(176..1660)
                     /locus_tag="VFX34_05620"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5564416.1"
                     /translation="MKTEQLLSTLFVKKIEGPLPDEVTDLAVNSRAVSAGGMFVCIEG
                     FTVDGHTFARSAADHGARVIVASKPVDVDTDKVAVVTVEDTSRAISQLAPAFYSYPSK
                     KLKTIGITGTNGKTSVSGIIHSVLRLAGKKSAVSGTIGFDLDGVLYESENTTNDVLTT
                     QQMISRAVNEGCEAMTMEVSSHGLVEGRLAGTEFDIAVFTNLTRDHLDYHGTMEHYGY
                     AKGLLFSQLGQDLSKPKFAVLNADDEWSNRYKEMTSHTVLTYGVTNDAIFRGSDIEYR
                     PDGTSFTLCSPDSEFRVNMSLIGEFTVYNALAAIAALSAYGMNTAEIVRLLGEIAPIK
                     GRMERVETDLPLTIFIDYAHTPDAIEKAISSVLPYKENKLIFLIGTGGNRDRKKRPIM
                     AEKASAADYVILTTDDPRHEPYESILAELEEGMLHKRYACIGDREAAVRHAIEVAEPG
                     DIILFAGKGHEDFQIIGSTKYPHSDADIALEEGMKKFGDLVKRK"
     gene            complement(1682..>2243)
                     /locus_tag="VFX34_05625"
     CDS             complement(1682..>2243)
                     /locus_tag="VFX34_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016082690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5564417.1"
                     /translation="GDFVSFDPRFEVTETGFVKSRHLDDKASTALVVELVRSLKEEGT
                     ELPYTTHFYISNNEEIGYGGNSNVPEQTVEYIAVDMGAIGDGQASDEYTVSICAKDSS
                     GPYHYRLTRHLVELCKTEGIPHKVDIYPYYGSDASAAIRAGYDVKHALIGPGIESSHA
                     LERTHVDSLKATASLLHAYVVSKMID"
BASE COUNT          588 a          569 c          429 g          657 t
ORIGIN      
        1 aaagtgcgcc gcgcagcaat ttcctctttt acgtttctca ttcgttccgt ctcctttttc
       61 ttagcttttc taatcataga gacaacaact tgatttttca atacttaatt accctaaaat
      121 tttcaattca tatgaatgaa aatagggaag caataatgct tccccacttt acaatctatt
      181 ttctcttcac caagtcaccg aactttttca ttccttcttc gagcgcgata tcggcatccg
      241 aatgtggata tttggtggag ccaataattt ggaaatcctc atgccctttg cccgcaaata
      301 gaatgatgtc tcctggttcc gctacctcga ttgcgtgacg gacggccgct tcccgatcgc
      361 cgatacatgc atatcgtttg tggagcatcc cttcctccaa ctccgcgaga atggattcat
      421 acggctcatg tcgaggatca tctgtcgtca agatgacata gtctgctgcc gatgctttct
      481 cagccatgat cggtcgtttc ttccgatctc ggtttccacc tgtaccaatt aagaaaatca
      541 atttattttc cttatacggc aggacgctgg aaatcgcttt ttcaatcgca tccggtgtat
      601 gggcatagtc aataaagatc gtaagaggaa gatcggtttc aacccgctcc atcctccctt
      661 taatcggagc gatttccccg agaagtcgga caatttccgc tgtattcatt ccatatgcgg
      721 ataaagccgc aatggcagcg agggcattgt agacggtaaa ctcgccgatt agactcatat
      781 tgacccgaaa ctcactatct ggggaacata gcgtaaacga tgttccatcc ggtcggtact
      841 caatatcact acctctgaat atcgcatcat ttgtaactcc atacgttaac acagtatggg
      901 acgtcatctc tttatatcgg tttgaccatt catcatccgc gttcaacact gcaaattttg
      961 gcttgcttaa atcttgacca agctgagaga acagaagtcc ctttgcatat ccgtagtgtt
     1021 ccatcgttcc atgatagtcc aaatggtcac gtgttaaatt tgtaaagacg gcaatatcaa
     1081 attctgtgcc agccaaacgt ccttcgacaa gtccgtgtga agaaacttcc attgtcatcg
     1141 cttcgcagcc ttcattcacg gcacggctga tcatttgttg tgtcgtcagt acatcatttg
     1201 ttgtattttc ggattcatat aatacaccgt ctaaatcaaa tccgattgtt ccggaaacag
     1261 cagatttttt tcctgccaac cgaagcaccg aatgaatgat tccacttacg cttgtttttc
     1321 catttgtacc ggtaatcccg attgttttca gtttttttga agggtacgag tagaaagctg
     1381 gtgccaattg gctaatcgcc cttgatgtat cttctaccgt aacaacagca accttatccg
     1441 tatctacgtc cactggcttt gaagcaacga tgacgcgagc accatgatca gctgctgacc
     1501 ttgcgaatgt atgcccatcg accgtaaacc cttcgataca aacgaacata cctcccgcac
     1561 tgacggcacg ggaatttacg gcaagatccg ttacttcatc cggtagcggc ccttcaattt
     1621 tcttcacgaa tagggttgat agcagttgtt ccgttttcaa aatgaaccct ccatttcaat
     1681 atcaatcaat cattttcgaa actacatacg catgcaacaa ggaagcagtt gctttcaagg
     1741 aatccacgtg cgtccgttcc agtgcatgcg atgattcaat ccctggtccg atcaatgcat
     1801 gctttacatc atagcctgcg cgaattgcag cagacgcatc ggaaccataa tatggataaa
     1861 tatcaacttt atgcggaatt ccttctgttt tacaaagctc gacaaggtgc ctcgtcaaac
     1921 gataatggta tggaccgctg gaatcttttg cacaaatgga gacagtgtat tcatcagacg
     1981 cttgtccatc tccaattgcg cccatgtcga cagcgatata ttccaccgtt tgttcaggaa
     2041 catttgaatt tccgccataa ccgatctctt cattattcga gatataaaaa tgagtggtgt
     2101 atggcagttc agtcccctct tccttcaatg atcgaaccaa ttccacgaca agggctgtgc
     2161 tagccttatc atctagatga cgcgatttca caaacccagt ttccgtcact tcaaaacgag
     2221 gatcaaagga cacaaagtcc cca
//
LOCUS       DASULI010000260         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1851829,
            whole genome shotgun sequence.
ACCESSION   DASULI010000260 DASULI010000000
VERSION     DASULI010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851829"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFX34_05630"
     CDS             complement(<1..486)
                     /locus_tag="VFX34_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX5564418.1"
                     /translation="MTLSERYQDLPLEEVIGDRFGRYSKYIIQDRALPDARDGLKPVQ
                     RRILYAMHVEGNTHEKAFRKSAKTVGNVIGNYHPHGDTSVYDAMVRMSQEWKLRHPMI
                     EMHGNNGSVDGDPAAAMRYTEARISAIAGEMLRDIGKETVDFIPNFDDTELEPTVLPS
                     RF"
     gene            complement(483..>2185)
                     /gene="parE"
                     /locus_tag="VFX34_05635"
     CDS             complement(483..>2185)
                     /gene="parE"
                     /locus_tag="VFX34_05635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX5564419.1"
                     /translation="PTTEVIFTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLEV
                     IIHRDGKKFIQRFEHGGKPATTLEPAGTTKETGTLIRFKPDPTIFSTVKYQYETLSER
                     LRESAFLLKGLKIVLKEEATGKEDIFHYESGIEAFVSYLNEEKDVLHEVVYFEGEQAG
                     IEVEFAFQFSDGYSETILSFVNNVRTKDGGTHETGAKSAMTRVFNEYARKIGLLKEKD
                     KNLDGSDIREGISAIVSVRIPEEILQFEGQTKGKLGTSEARTVTDAVVAQKLLYFLEE
                     NAELSASLVRKAIRAQQARDAARKAREDARSGKKRRKSETLLSGKLTPAQSRNAAKNE
                     LYLVEGDSAGGSAKQGRDRTFQAILPLRGKVINTEKAKLEDIMKNEEIATIIHAIGGG
                     VGADFNIDDIAYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYK
                     VFKGSGSKEKVEYAWTEADLEQAIKKIGKGYMLQRYKGLGEMNAGQLWETTMNPETRT
                     LIRVTIEDGAKSERRVTTLMGDKVEPRRKWIENNVDFGMLEDRNILDNEHLIVEEDYE
                     "
BASE COUNT          511 a          551 c          442 g          681 t
ORIGIN      
        1 gaatcgtgaa ggtaatacag ttggctcaag ctccgtatca tcgaagtttg gaataaaatc
       61 gaccgtctct tttccgatat cccgaagcat ttcgcctgct attgcggaaa tacgtgcttc
      121 cgtataacgc atagcagccg cagggtcacc gtctacggac ccgttatttc cgtgcatttc
      181 gatcattggg tggcgaagct tccattcctg gctcatccgc accattgcat cgtaaacaga
      241 tgtatcaccg tgtgggtgat agttaccgat gacgttaccg accgtttttg cggatttacg
      301 gaatgctttt tcatgagtat ttccttctac atgcatcgca tacaaaatac ggcgctgcac
      361 gggttttagt ccatcccggg catcaggtaa ggcgcgatct tgaataatat atttactata
      421 acgaccaaac ctgtcgccga tgacttcctc taacggcagg tcttgatagc gttcagatag
      481 cgtcattcat agtcctcctc aacaatcaaa tgctcattat caaggatatt ccgatcctcg
      541 agcattccaa agtctacgtt gttctcaatc cacttcctgc gtggctcaac cttatcgccc
      601 atcagcgtcg tgacccgtcg ttctgatttt gctccgtctt caattgtcac acgaatcaac
      661 gtacgggttt ccggattcat tgttgtttcc catagttggc cagcattcat ttcaccgaga
      721 cctttatatc gctgaagcat ataacctttc ccgatttttt taatcgcttg ttccaaatcg
      781 gcttcggtcc atgcatattc aaccttctct ttgctgccgg atcccttaaa cactttatag
      841 agaggtggaa gcgcgatata tacttttccg gcttcgatca atggcttcat atagcgatag
      901 aaaaacgtta acaggaggac ttggatgtga gcgccgtctg tatccgcgtc cgtcatgatg
      961 atgattttgt catatgcaat gtcatcaata ttaaaatcag ctccaactcc tccgccaatt
     1021 gcatggatga ttgtagcgat ttcttcgttt ttcataatgt cttctagctt cgccttttcc
     1081 gtattgatga ctttaccacg gagcggcaaa attgcttgga acgtacgatc tcttccctgt
     1141 tttgcggaac cacctgctga atctccctct actaggtaca attcgttctt tgcagcattt
     1201 ctcgattgtg caggtgtcaa tttaccggat aagagcgttt ctgatttccg gcgtttcttt
     1261 ccggaacgtg catcttcacg cgctttccgg gccgcgtctc ttgcttgttg tgcacggatt
     1321 gctttacgga caagagatgc acttagctca gcgttttcct caaggaaata aagcagcttc
     1381 tgcgcaacaa ctgcatctgt tacggttctg gcttcacttg tcccaagttt tcctttcgtt
     1441 tgcccttcaa actgaaggat ctcctcagga atacggactg aaacaatggc agaaatccct
     1501 tcacggatat cagaaccgtc aaggttttta tctttctctt tcaataaacc gatcttccgt
     1561 gcgtactcgt taaacacacg cgtcatagcg ctctttgcgc cagtttcatg tgtaccgccg
     1621 tcttttgtac ggacattatt tacgaatgaa aggattgttt cggaatagcc atcactaaat
     1681 tgaaatgcaa attcgacctc aatccccgct tgttctccct caaaatacac aacctcatga
     1741 aggacatcct tttcctcgtt caggtaggat acgaatgctt ctataccgga ttcatagtgg
     1801 aaaatatcct ctttaccggt ggcttcctct tttaaaacga tttttaatcc ttttaaaagg
     1861 aatgcggatt cacgtaaacg ttcactgagt gtttcatatt ggtatttaac agttgaaaat
     1921 atggtcggat ccggtttgaa gcgaatgagc gtaccagttt cttttgttgt accagccggt
     1981 tctaacgttg tagccggttt ccctccatgc tcaaatcgtt gaataaattt cttgccgtct
     2041 cgatgaataa tcacttccag ccattcggat agcgcgttca cgacagatgc gccgacgccg
     2101 tgcaatcccc cacttgtctt atagccgcct tgtccgaact ttcctccagc gtggagaacg
     2161 gtaaagataa cttcagtcgt tggtt
//
LOCUS       DASULI010000261         4892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_760195, whole
            genome shotgun sequence.
ACCESSION   DASULI010000261 DASULI010000000
VERSION     DASULI010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760195"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..520)
                     /gene="ispF"
                     /locus_tag="VFX34_05640"
     CDS             complement(44..520)
                     /gene="ispF"
                     /locus_tag="VFX34_05640"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5564420.1"
                     /translation="MFRVGQGFDVHKFEEDRPLIIGGITIPHECGLAGHSDADVLLHT
                     VTDAILGAIGEGDIGRHFPDTDAAFKDADSAILLEEIWKLAKERGYVLGNIDCTIIAQ
                     KPKMAPYIQAIRERIADLLESDASQVNVKATTTERLGFTGREEGIASMATVLLVRK"
     gene            complement(520..1212)
                     /gene="ispD"
                     /locus_tag="VFX34_05645"
     CDS             complement(520..1212)
                     /gene="ispD"
                     /locus_tag="VFX34_05645"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5564421.1"
                     /translation="MEYTVFIPAAGSGKRMGAGQNKLFLPLAGKPILIHTLEVFEKDP
                     ACQGIMLAVKPEERADIQRKLANFSITKVKALVDGGSERQHSVAACIHAYDRDGIVLV
                     HDAARPFIRRAVIHSLVKTAQESGAAIAAVRVKDTMKRVYDGTVEETVDRETLWIVQT
                     PQAFRYEVLKQAADSAIAEGFVGTDESMLVERVGFPVKIVESTYDNVKMTTKEDLVYG
                     EAILASRREEEI"
     gene            complement(1237..2340)
                     /locus_tag="VFX34_05650"
     CDS             complement(1237..2340)
                     /locus_tag="VFX34_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEX5564422.1"
                     /translation="MLKRVVQVLFLLIGGTLGVLFLPYLFKLFQFTSNPYVDNPYVTA
                     IIGAALFSLLSLLLTDPIVNFIRWMEDRLLKAPILDLIFGTVGLVIGLSVAFLASFGL
                     SSIQIPVVSAVLPVVLSVILGYLGFQVGFKKREDMIQSFQSIKHTGPKKKDGVDGTAA
                     ATSSVYKLLDTSVIIDGRIADIAETGFLEGVLVVPQFVLTELQHIADSSDTLKRTKGR
                     RGLDILKRLQTLKTPQVQIVDQDFADVQEVDMKLVRLAKDMNGQVVTNDFNLNKVSDL
                     HGVSVLNVNDLANAVKPVVIPGEHMHVVVIKDGKEQNQGVAYLDDGTMIVIEDGKTLI
                     GQATDVVVTSVLQTSAGRMIFAKPKNGKHVKVN"
     gene            complement(2710..4083)
                     /gene="radA"
                     /locus_tag="VFX34_05655"
     CDS             complement(2710..4083)
                     /gene="radA"
                     /locus_tag="VFX34_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5564423.1"
                     /translation="MAKKKTKFMCQSCGYETPKWMGRCPGCGQWNTMVEEVEIVSKGP
                     RGAFLHTVGIRQKAMPISAIETVEEPRIGTELGELNRVLGGGIVPGSLVLIGGDPGIG
                     KSTLLLQVSSLLANKGQRVLYISGEESVRQTKLRAERLGVLSDELYIYSETDLEHIHQ
                     TIEDVQPTFVIVDSIQTIHHPEVTSAPGSVSQVRECTAELMRIAKTRNIAIFLVGHVT
                     KEGQIAGPRLLEHMVDTVLYFEGERHHSYRILRSVKNRFGSTNEIAIFEMLQSGLKEV
                     LNPSELFLEERSKEAAGSTVVASMEGTRPILVEIQALVTPTSFNYPKRMATGLDQNRI
                     SLLMAVLEKRMGMLLQAQDAYIKVAGGVKLDEPAIDLAVLMSIVSSYRDTAVKTTDCF
                     IGEVGLTGDVRRVSRIEQRVGEAAKLGFKRAIIPASNIGGWEFPEGITVVGVETVRDA
                     LRVSFAE"
     gene            complement(4158..>4892)
                     /locus_tag="VFX34_05660"
     CDS             complement(4158..>4892)
                     /locus_tag="VFX34_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5564424.1"
                     /translation="AMIRVDMSEYMEKHSTSRLVGSPPGYVGFEEGGQLTEKVRRKPY
                     SVILLDEIEKAHPDVFNILLQVLEDGRLTDSKGRTVDFRNTVVIMTSNVGAEMLKRNR
                     YVGFNIQDGEKDYEDMKETMLEELKKVFRPEFLNRVDEMIVFHSLEKEHLREIVSLMS
                     NELVKRLKEQDIELTLTEAALDKIAQVGHDPDYGARPLRRALQKHVEDRLSEELLKGT
                     ALTGSKVIVDVEDGEFVVKTEAAAAK"
BASE COUNT         1281 a         1346 c          951 g         1314 t
ORIGIN      
        1 gtgatatttt atcattgccg gacaagtacg acaatggcac cgattatttt cttacaagaa
       61 gaaccgttgc catggaagca attccttcct cccgtccggt aaatccaagt cgttccgtcg
      121 ttgttgcttt tacattaacc tgggaagcgt ccgattccaa tagatccgca atccgttccc
      181 gaatggcttg aatataaggt gccatcttcg gcttttgcgc gataatcgtg caatcaatat
      241 ttccgagcac atagccgcgc tctttcgcaa gcttccaaat ctcctccaat aaaatcgccg
      301 aatctgcgtc cttaaatgca gcatctgtat ccgggaaatg acggccaatg tcgccttccc
      361 caattgctcc aaggatcgca tccgtaacag tatgcaaaag gacatccgca tctgaatgcc
      421 ctgccaggcc gcactcatgc ggtattgtaa ttccaccaat gatcagcggt ctatcctctt
      481 caaacttatg tacatcaaag ccctgcccaa ctctaaacat catatttcct cctctcgccg
      541 gctcgccaaa atcgcttcgc catacacgag atcttctttc gttgtcatct ttacattatc
      601 atacgtactc tcgactattt ttaccgggaa tccaactcgt tcgacaagca tggattcatc
      661 tgttccaaca aacccctctg ctatcgccga atcagccgcc tgcttcaaca cttcatagcg
      721 aaacgcctgt ggagtctgaa caatccagag cgtctccctg tcgaccgttt cctcaaccgt
      781 cccatcatag acacgcttca ttgtatcttt cactcgaacc gccgcaatgg cagctccact
      841 ttcctgtgcc gttttcacga gagaatgaat aactgctctt cgaataaacg gacgcgctgc
      901 atcatggaca aggactattc catcccggtc atatgcatga atacacgccg ctacactatg
      961 ttggcgctcg cttccaccat ccacgagcgc cttcaccttc gttatgctga aatttgcaag
     1021 cttacgctgg atatccgccc gctcttcagg cttcactgcc agcataatcc cctgacaagc
     1081 cgggtctttt tcaaacactt ccaaggtatg aatcaaaatc ggcttgcctg caagcggtaa
     1141 gaatagctta ttctgccccg ctcccatccg ctttccactt cccgccgcgg gaatgaatac
     1201 tgtatattcc acaatcatcg atccttcatc aaaaactcaa ttcactttta catgctttcc
     1261 gtttttcggt ttcgcgaata tcattcgacc cgcagatgtc tgaagtacac tcgtgacgac
     1321 aacatccgtc gcttgaccaa tcaatgtttt accatcctca atgacaatca tcgtgccatc
     1381 atcgagatat gcaacacctt ggttctgttc ttttccgtct tttatgacga cgacgtgcat
     1441 atgctcgcca ggtatcacaa ccggcttcac tgcatttgcc aaatcattga cgttcaaaac
     1501 ggaaacgcca tgcaaatccg atactttatt caaattaaaa tcattcgtga cgacttggcc
     1561 gttcatatcc tttgctagac ggacaagctt catgtccacc tcttgtacat ctgcaaaatc
     1621 ttgatcaaca atttgcacct gtggcgtttt caacgtctgc aaacgcttta aaatatccaa
     1681 tccacggcgc ccttttgttc gtttcagcgt atcagatgag tccgcaatat gctgaagctc
     1741 cgtcaagaca aactgcggta cgacgagaac cccttccagg aaaccggtct ctgcgatgtc
     1801 cgcaatccgc ccatcaataa taacactcgt atcaagtagt ttatacacgc ttgaagtagc
     1861 tgcggctgtc ccgtccactc catccttttt ctttggtcct gtgtgcttaa tggattggaa
     1921 ggactgaatc atatcttctc ttttcttaaa ccctacttgg aaacccaaat aacctaaaat
     1981 gacagacaaa acgaccggga gcaccgcaga aacaaccggg atttgaatgc tgctcaagcc
     2041 aaagcttgca agaaacgcga cactcaaacc aatgaccaaa ccgactgtcc cgaaaatcaa
     2101 atcgagaata ggcgctttta ataaccgatc ttccatccaa cgaatgaaat tcacaatagg
     2161 atctgtgaga agtaaactaa gaagagaaaa aagtgcggct ccaataatgg cagttacata
     2221 tgggttgtca acgtatgggt tggaagtaaa ctggaataat ttaaataaat acggtaagaa
     2281 taaaacccct aacgttccac caattaataa aaataaaacc tgaacaactc gcttcaacac
     2341 aagaacacct cctcgttgat gattatacat gtttgccctt acttacgcct gtttggccct
     2401 aagaaatagg ctgaaaatgg taagtttggc acatgtcgaa ttaaaaaaac ttgcttatga
     2461 agcagctata ctgacgacat accccctcca tttgttcctt tttcataaaa aacggctaca
     2521 acaaaagcgt aagtgggata acaatggagg cgtaatccat cctccaagtt atccaactta
     2581 tgacctcgag acctatgcac tgaagtctag acgtagattc cctataaaca ttcaaaacaa
     2641 cgggcatctc tatcctagcg tacacaaaat cgcacttact ttattcagaa aatgaaatac
     2701 cgatttcctt tattcagcaa aagaaacacg gagcgcatca cgaacagttt cgacgccgac
     2761 gacggtaatt ccttccggaa actcccaccc accaatattc gatgccggaa taattgcgcg
     2821 cttgaaccca agtttcgctg cctcccctac ccgctgctca atgcgggaga cgcgccgaac
     2881 atcgcctgtt aggccaactt ctccgatgaa acagtcggtt gtttttacag ccgtatcccg
     2941 gtagctcgac acgatactca tgagtacagc caggtctatc gccggttcat ccagtttgac
     3001 gccgccggct accttaatat acgcatcttg tgcttgtaac agcataccca ttcttttttc
     3061 tagaactgcc attaaaagcg atatccggtt ttggtcaagt cctgtcgcca tccgtttcgg
     3121 gtaattaaag ctcgttggcg tgacgagcgc ctggatttcc acgagaatcg ggcgagtccc
     3181 ttccattgat gcaacaaccg ttgaaccagc cgcttccttt gatcgctctt caagaaacag
     3241 ttcggacggg ttgaggactt ccttcaagcc gctctgcagc atttcgaaaa tagcaatctc
     3301 atttgttgaa ccgaatctat ttttaacgct gcgtaaaata cgatacgaat gatggcgctc
     3361 accttcgaag tacagaaccg tatccaccat gtgctcaagg agtcttggac ctgcaatttg
     3421 cccttccttc gttacgtggc ctacgaggaa aatcgcaatg tttcttgtct ttgcgatccg
     3481 catgagttcc gccgtacatt cacgcacctg cgacacgctc cctggggccg atgttacttc
     3541 aggatgatgg attgtttgaa tggagtcgac aatgacaaat gtaggttgaa catcttcaat
     3601 tgtctgatga atatgctcga ggtcggtttc ggaataaata tagagctcgt ccgataggac
     3661 accgaggcgt tccgcacgca gcttcgtctg ccgtaccgat tcttcgccgg aaatatagag
     3721 aacacgctgc cctttattcg caagcaacga ggatacttgc aagaggagcg tagacttccc
     3781 aattccagga tcaccgccga tcaaaacaag agagcctggt acaatcccgc cgccaaggac
     3841 ccggttcagt tcaccgagtt cggttccaat ccgcggctct tcaacagttt cgattgcact
     3901 gatcggcatt gccttttgtc gaataccaac cgtatgcaga aatgcccccc gcggtccttt
     3961 ggagacaatt tcaacttctt ccaccatcgt attccattgt ccacaccccg ggcaacgtcc
     4021 catccacttt ggtgtttcat acccacaaga ttgacacata aactttgttt ttttcttagc
     4081 catattatcc cccattaaaa aaggacatcc cggtggcgcc ggatgcttcc ggcgggcgga
     4141 atgtcccgtt cgtagtttta cttcgcagca gctgcttcag tttttacgac aaattcaccg
     4201 tcttcaacat caacaataac cttgctacct gtgagcgctg tccctttcag caattcttca
     4261 gacaagcggt cttccacatg tttctgaagt gcgcggcgga gcggacgtgc tccgtaatcc
     4321 ggatcgtgtc caacttgcgc tattttatcg agcgccgctt cggtcaatgt tagctctata
     4381 tcctgctcct tgagtcgctt cacaagctca tttgacatga gtgaaacgat ttctctcaag
     4441 tgttcttttt cgagtgagtg gaagacaatc atttcatcga cacggttcaa gaactccgga
     4501 cggaatactt tcttcaactc ttccaacatt gtttctttca tgtcttcgta gtccttctcg
     4561 ccatcctgta tgttgaaccc gacatagcga ttgcgtttca acatttccgc tccaacgttg
     4621 gatgtcataa tgacaaccgt atttcggaaa tcaaccgtgc gacccttcga gtccgttaaa
     4681 cgaccatcct ccagtacttg taggagaata ttgaatacat ccgggtgcgc tttctcaatt
     4741 tcatcgagaa gaatgacgga atatggctta cggcgtactt tctccgttaa ttgaccgcct
     4801 tcttcaaatc cgacatagcc cggaggggac ccaacaaggc gggaagtcga atgtttctcc
     4861 atgtattcag acatgtcgac acgaatcatc gc
//
LOCUS       DASULI010000262         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_196701, whole
            genome shotgun sequence.
ACCESSION   DASULI010000262 DASULI010000000
VERSION     DASULI010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196701"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1951
                     /locus_tag="VFX34_05665"
     CDS             <1..1951
                     /locus_tag="VFX34_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017186269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX5564425.1"
                     /translation="VDAQDILETFDKENQFRIEIGRWAYVVTFLAVALTVFHLYTGAT
                     SVLPSQQQGAIHLGTALGIIFLLYPAKKEWRKTQKKVPWYDVLLAFTAMYVAYHKIIF
                     FDSILQSRVSGYSTIDIIISVLGVLLVLEATRRTVGVPIVIVAIIAMLYAMYGNFIPT
                     KILSHQGFAVDRTMTTLWYRESGVFGKPIQISAKFIFLFLFFGVLLVNTPIGRFFNNL
                     AFSLTGRYTGGTAKAAVVASALQGTVSGSSVGNTVATGSFTIPMMKKAGFKPEFAAAT
                     EASASTGGQIMPPMMGAAAFIMMEYLGVSYANIMLAALIPAILYFTGIFIGTHFEAKR
                     LKILGLPKSELPVFKKIFVRDGYMIIPLLVIIVTIMSGFTPQRAALFGILSAVIVWIF
                     NAIYRKESEFGVRRIIYVLEQGGRVALPVISAVATAGIIAGVVSMTGLGAKFASGIIA
                     LSNGYLILALFFTMIACIVLGMGLPTTANYVVTATIAAPALINEFGIAPIAAHMFVFY
                     FDIVADITPPVCLAAYAGAGIAKANPFKTGMTAVKLAIAAFIIPYIFIYNPILVFVDV
                     TPVKLILGIATALIGMIGVSSAVIGYFVRDARIWERIVLFAGGLLLIIPELTTSAIGL
                     LLIAIIWVIQRRRPEEKQSIKEVVA"
BASE COUNT          493 a          534 c          551 g          520 t
ORIGIN      
        1 ggtagatgcg caggatatcc ttgaaacgtt cgataaagaa aatcaattcc gtattgaaat
       61 cggcagatgg gcatacgtcg tcaccttcct ggcagtggcg cttacggtat tccatctcta
      121 tacaggggcc acgagcgtcc tgccgtcgca gcagcagggg gccatccacc tagggactgc
      181 actcggaatc atcttccttc tctatccggc aaaaaaggaa tggagaaaaa cgcagaaaaa
      241 agtcccgtgg tatgatgtac tgctagcgtt tacggcaatg tacgtggctt accataagat
      301 catctttttc gattcgattc tgcaaagcag ggtttccggc tacagcacaa tagatattat
      361 catttccgta cttggcgtat tgctcgtatt ggaggcgaca agaagaactg tcggcgtgcc
      421 gatcgtcatc gttgccatca tcgccatgct gtatgcgatg tacgggaatt tcatccctac
      481 gaaaatctta tcccatcaag gctttgcggt ggaccggaca atgacgacgc tctggtatcg
      541 cgaaagtggt gtattcggca agccgataca gatctccgcg aaattcattt tcctgttcct
      601 gtttttcggg gtgctactcg tcaatacgcc gatcgggagg ttcttcaaca atctggcatt
      661 ctcgttaacc gggcgctata cagggggaac cgccaaggct gccgtcgttg caagcgcgct
      721 gcaggggacg gtatccggaa gctccgtcgg caacacagtg gcgacgggaa gcttcacgat
      781 tcctatgatg aaaaaggcag gattcaagcc tgaatttgcg gcggctacgg aagcatccgc
      841 ttcgaccggc ggccaaatca tgccgccgat gatgggggct gcggccttca tcatgatgga
      901 gtacctcggc gtcagctatg caaatatcat gctggccgcg ctcattccgg caatcctgta
      961 cttcaccgga attttcatcg ggacccattt cgaagcgaag cgcctgaaga tactggggct
     1021 gcccaagtcg gagctgccgg tattcaaaaa gatctttgtg cgggacgggt acatgatcat
     1081 tcctctcttg gtcatcattg ttacaatcat gtccggcttc acaccgcagc gtgcagcctt
     1141 gttcggaatc ctttccgccg tcatcgtatg gattttcaat gcgatctata ggaaggagtc
     1201 tgaattcggt gtaagaagga tcatttatgt attggagcaa gggggaaggg tagcgcttcc
     1261 agtcatttca gctgtagcaa cagccggtat catcgcaggt gttgtcagca tgacgggctt
     1321 gggcgcgaaa ttcgcctccg gcattatcgc cctttcgaac ggctacttga tactggcact
     1381 attcttcacg atgattgcat gcatcgtgct cggaatggga ttgccgacaa cggccaacta
     1441 tgttgtcact gcaacgattg cggcaccggc gctcatcaac gaattcggca tcgcgccgat
     1501 tgctgcgcat atgttcgtct tctatttcga catcgtcgcc gacataacgc ctccggtctg
     1561 cttggcggcc tatgcggggg ccgggattgc aaaggcgaat ccgttcaaga cgggaatgac
     1621 tgctgtaaag ctggcgattg ccgctttcat cattccgtat atattcattt acaatccaat
     1681 cctcgtcttc gtcgatgtga cgccggtaaa gctcatactt ggcatcgcca ccgcattgat
     1741 cggcatgatc ggggtcagca gcgccgtcat cgggtacttc gtacgggatg cacgcatctg
     1801 ggaacggatt gtcctgttcg cgggaggtct gctgctcatc ataccggaac tgacaacgag
     1861 tgccattggc ttactgctca ttgcaatcat ctgggtcatc cagagaagac gaccggaaga
     1921 aaaacagtct attaaagaag tcgttgcata agggaataaa caaaaagctg tcacctggaa
     1981 aatgggtgac agctttttgc ttgaaatcgg ttctgcgctc ataaacgctg catccgcgct
     2041 cataaattcg aaatcgcgat cataaacttg gaaatacgct cataaatgcc gcatccgc
//
LOCUS       DASULI010000263         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1253649,
            whole genome shotgun sequence.
ACCESSION   DASULI010000263 DASULI010000000
VERSION     DASULI010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253649"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1129
                     /locus_tag="VFX34_05670"
     CDS             380..1129
                     /locus_tag="VFX34_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5564426.1"
                     /translation="MITLHNVSKSFSFFQAIQSVSLSIEKGEIHGIIGASGAGKSTLL
                     RLMNLLEVPDEGEVAVNGQTLTNLSGKQLRQARKSIGMIFQHFHLVANKTVYENVVAA
                     LELAKFPRKARRPRVEECLRFVGLGNFIDKYPAQLSGGQKQRVAIARALANNPQVLLC
                     DEPTSSLDPNTTAEILSVLEDINQSFGVTIVIVSHEMEVIKSICNRVTVLNEGQVYDT
                     VSIEPKGIGMKNNSPSFFVEQLTKDGESSHA"
     gene            1122..1784
                     /locus_tag="VFX34_05675"
     CDS             1122..1784
                     /locus_tag="VFX34_05675"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HEX5564427.1"
                     /translation="MPDILLQYEAEIWRSIGETFIMVGVSIAAAVIVGLPVGTLLFLC
                     RKGQIYENRIVFSVLNLLVNIIRSFPFLLLVVFLIPFTRMLIGTAMGTTAATVPLSII
                     AIAHYSRLVEQSLVDVPKGVMEAAIAMGASVRQVIFKFLYVEARSGLVLGLTTSIISF
                     ISYSTIMGVVGGGGIGDFAIRYGYQQFKDDLMMYMIIIMVILVQFIQFTGTTVSRMID
                     KR"
     gene            1799..>2045
                     /locus_tag="VFX34_05680"
     CDS             1799..>2045
                     /locus_tag="VFX34_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5564428.1"
                     /translation="MRKLLMLTALLMVLLAGCGKDKAKDTPAEVQEKEEEVTLKVASL
                     IPPMTEILELVKPTLADEGIDLEIVVLGDNVQPNSALA"
BASE COUNT          563 a          422 c          504 g          556 t
ORIGIN      
        1 ccgatcattt cctcgccaag atagattcct ttggcgtgga agttttcaag gaaattcaac
       61 tgagccagtg acacagcgtt cgacgcgacg aaattcactt ggtcctcccg tacgcgaagt
      121 gaaatagcct tcacccaatt gtctttcgtg attgtttcca aatgcagcat gatcatctcc
      181 ccttctccta gattatagtc gctgatctgg caatctagga agcctgatta tttaaaatag
      241 gaaatttttt taacatattg gtcaactgtt tgaaattaac aataatatta tgcaaacgat
      301 aatatgacaa tctaggcaaa ttaggctata atagtaaacg tcgtaatcac ccttcaaatc
      361 gtggaaaggg gattttttta tgattacatt acataatgtg agcaaaagtt tctcgttttt
      421 tcaggctatt caatcagtct ctttatcaat agaaaaaggc gagatccatg gcattatcgg
      481 tgcaagcggc gccggcaaat caacgcttct gcgattgatg aatttattgg aagttccgga
      541 tgaaggggaa gtggcagtca atggccagac gctgacaaat ctgagtggca aacaactccg
      601 gcaagctagg aaatccattg ggatgatctt tcagcatttt catttggtag cgaataaaac
      661 ggtatatgaa aacgtcgttg cagcgttgga gctggctaaa tttccgagaa aagcgcgccg
      721 gccccgtgtt gaagaatgcc ttcggtttgt cgggttgggt aatttcatcg acaaatatcc
      781 tgctcaatta agtgggggac aaaagcagcg ggtggcgatt gcgagagcat tggcgaacaa
      841 tccgcaagtg ctattatgcg acgagccgac ttcatctctt gatccgaata caaccgctga
      901 aatattgagc gtgctggagg acatcaatca aagcttcggc gttacgattg taattgtcag
      961 ccatgagatg gaagtgatca aaagcatctg caaccgcgtc acggtcttga atgaaggaca
     1021 agtgtacgac acggtttcca ttgagccgaa agggattgga atgaagaata atagcccaag
     1081 tttcttcgtg gaacagctga caaaggacgg tgaatcgagc catgcctgat attttgcttc
     1141 aatatgaagc tgagatttgg cgttcgattg gggagacgtt catcatggtg ggggtttcga
     1201 ttgccgcggc cgtaattgtc ggacttcctg taggaacatt gcttttccta tgcaggaaag
     1261 ggcagatcta cgagaaccgg attgtctttt cggtattgaa tttattggtg aatatcatcc
     1321 gttcattccc attcctctta ttggtcgtct ttctcatccc gttcacgagg atgctcatcg
     1381 gcactgccat ggggactaca gctgcgactg tgccgttgtc aatcatcgct attgcacatt
     1441 actcgcgatt ggtcgagcaa tcattagtcg acgtgccaaa aggtgttatg gaggcggcaa
     1501 ttgcaatggg agcatcggtc aggcaagtca tcttcaaatt cctttatgtc gaagcccgct
     1561 ccggactcgt tttagggttg acgacgtcga tcatcagctt catttcctat tcgacgatca
     1621 tgggcgtcgt cggtggcggc ggcattgggg actttgcaat ccggtacggc taccagcaat
     1681 tcaaggacga tttaatgatg tacatgatta tcattatggt catactcgtg cagttcatcc
     1741 aatttaccgg aacgactgta tcaagaatga tagataaacg ataacaggag gaaacaacat
     1801 gagaaaacta cttatgctga cggcattgtt gatggtttta ttagcaggtt gcggcaagga
     1861 taaagcaaag gatacccctg ctgaagtgca agagaaagag gaagaagtga cgttgaaagt
     1921 cgcttcattg attcctccaa tgacggaaat ccttgaactc gtcaaaccga cacttgctga
     1981 tgaaggaatc gaccttgaaa tcgttgtgtt aggcgataat gtacagccga acagtgcact
     2041 cgcgg
//
LOCUS       DASULI010000264         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1375906,
            whole genome shotgun sequence.
ACCESSION   DASULI010000264 DASULI010000000
VERSION     DASULI010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375906"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFX34_05685"
     CDS             <1..253
                     /locus_tag="VFX34_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HEX5564429.1"
                     /translation="VVRNMTESLTNATYGVLYSEEGDGFINREFLAKHVAEGSDVYVC
                     GPTPFMQAVISALHQIGFPEEKIHFEFFGPKEQLELVNA"
     gene            complement(313..1368)
                     /locus_tag="VFX34_05690"
     CDS             complement(313..1368)
                     /locus_tag="VFX34_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010648664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564430.1"
                     /translation="MKKILKMGSAFIGIIVGAGFASGQEILLYFTSFGVMGIVAAVLC
                     TILFAYLGMVLTRVGSRMRTTSHKEVIYKISGRYLGLIIDYVIIFTLFGTGVVMLAGA
                     GSNLNQQFGLPPIVGTLLMVILVFITILLNVDKVVAVIGSITPFLILAVVVISVYSIA
                     TMDESFSALNSTATAIHTTLPNWFISAINYVSFNIAVGASMALVMGGVEKDEKTAALG
                     GLVGGIGLGIMILLSHLAIFSKVDVVGHADMPMLAIINEISPVLGIFMAFILYGMIFN
                     TAVSMFYAFGARFVKIGTKKFEIFVLITLLVGFALSFRGFKELVGSFYPIVGYAGLFL
                     VGALIVASFRLPAKVRK"
     gene            complement(1569..2081)
                     /locus_tag="VFX34_05695"
     CDS             complement(1569..2081)
                     /locus_tag="VFX34_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX5564431.1"
                     /translation="MAKIATVITNLFEDSEYTEPAKAFEEAGHEVVTIEKEAGKEVKG
                     KQGESTVKIDVGIGDASPDDYDALFIPGGFSPDQLRADDRFVQFAKAFMDEKKPVFAI
                     CHGPQLLLTAKTLEGRDATGYKSIKVDMEYAGAKYADKEVVVCQNQLVTSRTPDDLPA
                     FIRESNKLLK"
     gene            complement(2146..2745)
                     /locus_tag="VFX34_05700"
     CDS             complement(2146..2745)
                     /locus_tag="VFX34_05700"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5564432.1"
                     /translation="MLLYKPVDKSFFEPHVLELARNLVGQYIVHERPDGVIAARIVET
                     EAYHGPEDRAAHSFGNRRTKRTEIMFGTAGLVYTYQMHTHTLMNVVSGPIGTPHAVLI
                     RAAEPVEGFELMQENRGPHLKMKDWTNGPGKLSKALGVTMEYYGHHWADEPLFIAVGP
                     GAGEIETGPRVGIGNTGEAVHYPWRFFEKDNPYVSKYRP"
BASE COUNT          782 a          775 c          540 g          717 t
ORIGIN      
        1 agtagtgcgc aacatgaccg aatcacttac aaatgcaaca tacggagtac tttattccga
       61 agaaggcgat gggttcatca accgtgaatt ccttgcgaaa catgtagcgg aaggcagcga
      121 cgtgtacgtc tgcggaccga ctccattcat gcaagcagtc atctctgcat tacaccaaat
      181 cggattccca gaagaaaaaa tccatttcga attcttcgga ccgaaagaac aattggaact
      241 agtaaatgct taagtaatgg ttgtacacaa aaagcgcgcc tttcttaaat gaaagagacg
      301 cgctttttac tcttatttac gaactttcgc cggtagcctg aaagacgcga caatgagtgc
      361 tccgacaagg aataaaccgg catacccgac gatcggatag aaacttccaa ctaactcttt
      421 aaatcctcga aagcttagtg caaatccgac aagcaacgta atcaaaacaa aaatctcgaa
      481 ttttttcgta ccaatcttga cgaacctcgc accgaaagca taaaacatgc ttacagccgt
      541 attaaagatc attccataca agatgaatgc catgaaaatg ccaagcaccg gcgagatttc
      601 attaatgatt gccagcattg gcatatccgc atgccccact acatccactt tggagaagat
      661 tgccaaatgg cttagcaaaa tcataatacc caagccaata ccaccgacca atccaccaag
      721 ggcagcggtt ttctcatcct tctctactcc ccccatgacc aaggccattg aagcacctac
      781 agcaatgttg aaggatacgt agttgattgc tgaaatgaac cagttcggca aagtcgtatg
      841 tatagctgtt gccgttgaat tcaatgcgct aaatgactca tccatcgtag ctatcgagta
      901 aacagaaatg acaacaaccg ccaggatcag gaacggcgta atacttccta taacagcaac
      961 aaccttatca acattcagca atatcgttat aaagacaagt ataaccatca ataaagtgcc
     1021 tacgataggc ggaagtccaa attgctgatt caagttagaa cccgctcctg ccaacattac
     1081 gactcctgtc ccgaacaacg tgaaaataat gacgtagtca attattaatc cgagatagcg
     1141 gccgctgatt ttgtaaatga cttccttgtg ggacgtcgtt cgcatccggc ttccaaccct
     1201 cgttaacacc attcccaaat aagcaaatag aattgtgcac aatacagctg ccacaattcc
     1261 catcacccca aaacttgtga agtacaatag aatttcctgg ccggaagcaa aaccggcacc
     1321 gacaatgatc ccgatgaacg cactacccat tttcagtatc tttttcatac tgtttttccc
     1381 ccttggtgtc cccgtaaaaa acctttcata tgccaaataa tccgaatttt cgacgcgaag
     1441 aaatttgtat tgttttaatt taacacgcga ttcacctcta ttcaatatgc tattttcttt
     1501 atttttatat tccggtatcc agcaaaaaag aaccgccccg gtcgagggac ggtcctacat
     1561 tacaatcatt acttaagtaa tttattcgat tcgcggatga acgccggtaa atcatcaggt
     1621 gtccggctcg tcaccagttg attttggcac acgacaactt ctttatccgc atacttcgca
     1681 cctgcatatt ccatatcgac ttttatggat ttatagcctg ttgcatcgcg gccttccaac
     1741 gtcttcgccg tcagcagcag ctgaggtcca tggcaaatgg cgaaaaccgg tttcttttca
     1801 tccatgaacg cttttgcgaa ttgaacgaat cgatcgtccg cccgcagctg atccggcgaa
     1861 aatccgcccg gaatgaacag ggcgtcatag tcgtcaggcg aagcatcgcc gattccgacg
     1921 tcaatcttga ccgtcgactc cccttgcttg cccttcactt ccttgccggc ctccttctcg
     1981 atcgtgacaa cttcatgtcc cgcttcttcg aacgcctttg ccggctccgt gtactctgag
     2041 tcttcgaata ggttcgtgat tactgtagca attttagcca ttctagcatc caccctttcg
     2101 tttatgatca ccttactact attccacagt agtgcgacac taaacttatg ggcggtactt
     2161 tgagacataa ggattatcct tttcaaaaaa ccgccatggg taatggacgg cttcaccggt
     2221 atttccaatt cctacacggg gacccgtttc aatctcgcct gctcccggcc caacggctat
     2281 gaaaagcggc tcgtccgccc aatgatgccc ataatactcc atcgtcacac cgagcgcttt
     2341 cgacagcttc ccaggaccgt tcgtccaatc cttcatcttc aaatgcgggc cccggttttc
     2401 ctgcatcagc tcaaaccctt caaccggctc tgcggcgcgg ataagaacag cgtgcggcgt
     2461 tccgatcggt ccactgacga cattcatcaa tgtatgggtg tgcatttggt acgtgtacac
     2521 aagtcccgca gtgccgaaca tgatctccgt ccgcttcgtc cgccggttcc cgaaactgtg
     2581 ggcagcacga tcctcaggac catgatacgc ttccgtctca acaatacgtg ccgcaatcac
     2641 cccatccggc cgctcatgca caatatattg cccgacaaga ttccgcgcga gttcaagcac
     2701 atgcggttca aaaaatgatt tatcaaccgg cttgtacaac agcatccctc cgtccgtgca
     2761 aaactcacca tattttacag tgatctcgtt atgattaact tcctatacat cgct
//
LOCUS       DASULI010000265         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1909598,
            whole genome shotgun sequence.
ACCESSION   DASULI010000265 DASULI010000000
VERSION     DASULI010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909598"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..635
                     /locus_tag="VFX34_05705"
     CDS             123..635
                     /locus_tag="VFX34_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5564433.1"
                     /translation="MILSNTGKYTVVSLLVNGTSHDIMIRSADTLLCTLRNQLGLTGA
                     KQACGTGDCGACTVLIDGKPMQSCLTLTLESLNSGITTIEGLKHSPVRKAFVDNWAIQ
                     CGYCTPGFIVNGHALVTAHPDADDKVIEEWLQSNLCRCTGYQEIKDAIQSALAASKLM
                     ETTGNDNTGQ"
     gene            complement(682..1005)
                     /locus_tag="VFX34_05710"
     CDS             complement(682..1005)
                     /locus_tag="VFX34_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003329419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564434.1"
                     /translation="MVQQNTQQQTIEQQFQTEKKQILQKLQKASQTIQQMSTMFDAQS
                     VQQAQQQLQQASQLLNQLQGAAATQSPSTVQQQLEQVRKQIDQAAQTLQLIETLNSGN
                     KSFKK"
     gene            1142..1858
                     /locus_tag="VFX34_05715"
     CDS             1142..1858
                     /locus_tag="VFX34_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEX5564435.1"
                     /translation="MGIGSGVDSQFVMFSFVHISALVLITFIVFLLFSQRKRLQMNVP
                     RLKKAEHTFGLSLLAIDITYHLWLISTGRWGLDDALPLELCSISLFVSVVLLLTGNRH
                     LIDFVIFAGIGGALQALATPVLDMNFPHFRFFHFFYTHAGIILTGLYFVWVKGYEPTF
                     KGVVKTMLIINALLPCIIAINWLVDGNYMFLHMKPQNGSLLDFLGPYPWYILSLEAMA
                     FIIFTVIWLLLRKKDTKIEN"
     gene            1951..2142
                     /locus_tag="VFX34_05720"
     CDS             1951..2142
                     /locus_tag="VFX34_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564436.1"
                     /translation="MESNKQSGEKAKISLKDAVKQQLEAKKNKTQSTKTGGNPIQQTP
                     KMKSQQSKKVSQSRRKMGV"
     gene            complement(2190..2654)
                     /gene="ribE"
                     /locus_tag="VFX34_05725"
     CDS             complement(2190..2654)
                     /gene="ribE"
                     /locus_tag="VFX34_05725"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5564437.1"
                     /translation="MSLTYEGHLIGTGLKIGIVVSRFNEFISGKLLGGAEDALRRHGV
                     DSEDIEIAWVPGAFEIPLIAKRMAASKKYDAVITLGTVIRGSTPHFDYVCNEVAKGVA
                     ALNMTEGVPVIFGVLTTDSIEQAVERAGTKAGNKGWDAGVSAIEMANLLKQL"
     gene            complement(2669..>3100)
                     /locus_tag="VFX34_05730"
     CDS             complement(2669..>3100)
                     /locus_tag="VFX34_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5564438.1"
                     /translation="CLTGDVFGSCRCDCGPQLHAALAQIEEAGAGILVYMRQEGRGIG
                     LVNKMKAYQLQEQGYDTVEANAQLGFGDDLREYFISAQILADLGCTRIQLLTNNPRKI
                     DGLSEHGITIVNRIPIELPVEDDNRFYMETKKTKLGHLLHN"
BASE COUNT          789 a          702 c          719 g          890 t
ORIGIN      
        1 gaaaaggcga ttgccaaatt gccaacgaac attaaagatg atttcgtggc ttcaaaagac
       61 ttccgccgat ttatcctgat taagctatta accgaaatga gcagtgcatt ggaggagatt
      121 tgatgatcct atcgaatacc ggcaaatata cagtcgtttc tttacttgtc aatgggacat
      181 cacatgacat catgatccgg tcagcagaca cattgctatg tacgttgcgg aatcaacttg
      241 gtttgaccgg cgcgaagcaa gcttgcggca ctggagactg cggggcatgt accgtattga
      301 tcgacggaaa accgatgcaa tcatgcctta cattgacact tgaatctttg aacagcggaa
      361 ttacgacgat agaaggattg aaacattcac cggtgaggaa ggcattcgta gacaactggg
      421 cgatccaatg cgggtattgt acaccgggat tcatcgtcaa tggccatgcg cttgtaacgg
      481 cccatccaga cgcggacgac aaagtgattg aagagtggct gcaatcgaat ctttgcagat
      541 gcaccggcta ccaggaaata aaagatgcaa tccaatccgc ccttgcggca agcaaactga
      601 tggaaaccac aggcaatgac aataccggac agtaaaaaat gaacccttgg tgtttggatt
      661 gccaagggtt catttctatt tctatttttt gaaactctta ttgccgctgt tcaacgtttc
      721 aatcaactgc aatgtttggg ctgcttggtc gatctgtttc cgcacctgtt ccaactgttg
      781 ctgtactgtg gacggcgatt gtgttgccgc cgccccttgc agctgattca aaagctggga
      841 cgcctgttga agctgctgct gagcctgctg gaccgactgt gcatcaaaca ttgtactcat
      901 ttgttgtatc gtctgcgacg ctttttgcaa cttttggaga atctgttttt tctccgtctg
      961 gaattgttgc tcgattgtct gttgctgggt gttttgttgg accatccttt tattcccctt
     1021 tcgttccaat tgctcatagt atgtgtgtat cgaaacaagg ttaaacaaac tattcttttt
     1081 atccttgttg agcgatgcta taatttgggg taatcattat agaccggaat ggggaatggc
     1141 agtgggtatc ggcagtgggg tggattctca gttcgtcatg ttttcttttg tgcatatttc
     1201 ggctcttgtc ttaatcacgt tcatcgtttt cctcttattt agtcaacgga agcgattgca
     1261 aatgaacgtt ccccgattga aaaaggcgga acatactttt gggctctctt tattggcaat
     1321 cgatatcact tatcacttat ggctcatttc aacaggccgg tggggactgg atgacgcgct
     1381 gccgcttgaa ttatgcagca tctctctgtt cgtctcggtt gtgctcttgt tgacggggaa
     1441 tcggcatttg atcgacttcg tcatctttgc gggaattggc ggcgctctgc aagcgttggc
     1501 aacacctgtt ctggatatga atttcccgca cttccgcttc ttccattttt tctatacgca
     1561 tgcagggatc attttgacgg gactttattt tgtgtgggtg aaaggctatg agcctacgtt
     1621 taaaggcgta gtgaaaacaa tgttgatcat caatgcgcta ttgccttgta ttattgcgat
     1681 caactggctc gttgatggga attatatgtt tcttcacatg aaaccccaaa atggaagtct
     1741 acttgatttt ctaggccctt acccttggta tatattgtcg ctggaagcga tggcattcat
     1801 tatattcacg gtcatttggc ttttattgcg gaagaaggat acaaagatag aaaactaact
     1861 ttggtaattt tgtgtatatt ctttgtggac aattattcat cgagaatgct aaaatttact
     1921 aaccgatagc aacgggaggg tgaaagcgaa atggagtcga acaagcaatc aggggaaaaa
     1981 gcgaaaatca gcttgaaaga cgcagtgaaa cagcagcttg aagcaaagaa gaataaaacc
     2041 cagtcaacaa aaacgggtgg aaacccgatt caacaaacac cgaaaatgaa aagccaacag
     2101 tcaaagaaag ttagccaatc ccgccggaaa atgggtgtct gatcacaaaa agcaagtccc
     2161 tgcaaatcgg gacttgcttt tggtttttct tataactgtt tcaataggtt tgccatctca
     2221 atggcggata ctcctgcgtc ccatccttta ttcccagcct tcgtaccggc acgttcaacc
     2281 gcctgttcga ttgaatcagt cgtcaatacg ccaaaaatga cgggaacgcc ttctgtcata
     2341 ttgagtgcgg caaccccttt cgccacttca ttgcatacat agtcaaagtg tggggtagaa
     2401 ccgcgaataa cggtgcccaa tgtaatgacc gcgtcatatt tctttgaagc cgccatccgc
     2461 tttgcaatga gggggatttc aaaagcgcca ggcacccatg cgatttcgat gtcttccgag
     2521 tcaactccgt ggcggcgcaa tgcatcttcc gctccaccaa gtaattttcc tgaaatgaat
     2581 tcattgaatc gggatacgac gattccgatt tttagtcctg ttccgattaa atgtccttcg
     2641 tatgtcaaac tcattttgca tttcctcctt agttgtgcag caaatgtcct aatttcgtct
     2701 ttttcgtctc catatagaac cggttgtcat cttcgacagg caactcgatt gggatccgat
     2761 tcacgattgt tatcccatgt tcagaaaggc catcgatttt acgtggattg ttcgtcaaca
     2821 attgaatgcg ggtgcaccca agatcggcta ggatttgcgc gctaatgaaa tactcccgca
     2881 gatcatcacc gaaccctaat tgggcattcg cttcgacggt gtcataacct tgttcctgca
     2941 gctggtaggc tttcatttta ttgacgaggc cgattccacg gccttcctgg cgcatataga
     3001 cgagaattcc ggccccagcc tcctcgattt gggcaagtgc agcatgaagc tgcgggccgc
     3061 aatcacaacg gcaagaaccg aacacgtcgc ccgtcaagca
//
LOCUS       DASULI010000266         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1069187,
            whole genome shotgun sequence.
ACCESSION   DASULI010000266 DASULI010000000
VERSION     DASULI010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069187"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /gene="thrS"
                     /locus_tag="VFX34_05735"
     CDS             complement(<1..1662)
                     /gene="thrS"
                     /locus_tag="VFX34_05735"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX5564439.1"
                     /translation="MAENIQLKFPDGAIKEFPKGTTTEEVAGSISPGLRKSALAGKIG
                     DQLIDLKTPIENDGEIAIITPQSPEALEILRHSTAHLLAQAVKRKFPDAKLGIGPVID
                     NGFYYDIDSPTPITAEDLPELEKEMKRIIGENLPITRIDVSREEAEKRFKEIEDPYKL
                     ELLEAIPEGEQVSIYEQGEFFDLCRGIHVPSTGKLKEFKLLSIAGAYWRGDSKNKMLQ
                     RIYGTAFFKKDELQEHLRMLEEAKERDHRKIGKELNLFTNSQKVGQGLPLWLPKGATI
                     RRIIERYIVDKEVKLGYDHVYTPVLGSVELYKTSGHWGHYQDGMFPVMSMDNEDLVLR
                     PMNCPHHMMIYKNGIHSYRNLPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFV
                     RPDQIKEEFKRVVQLVIDVYKDFNIEDYSFRLSYRDPEDTEKYFDDDEMWERAQSMLK
                     ETMDELGLPYVEADGEAAFYGPKLDVQVKTAIGMEETLSTVQLDFLLPERFDLTYVGE
                     DGKHHRPVVIHRGVVSTMERFVAFLIEEYKGAFPTWLAPVQVEVIP"
     gene            complement(2014..2961)
                     /gene="dnaI"
                     /locus_tag="VFX34_05740"
     CDS             complement(2014..2961)
                     /gene="dnaI"
                     /locus_tag="VFX34_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEX5564440.1"
                     /translation="MERIGNTMKRVFNASDLTARYEQLRNEVMESPGVQQFISEHSNE
                     IDKAVVDKSLGKLYEYATASHHCEKCPNIEGCINVMKGFEPKLVLAQGFIDLDYIKCP
                     NKLVDDERRNVSNMIDSMYMPKDVMEARLHDVDDLQHNDSRYIIVEAAEQFLSEIRKT
                     GELPERGLYIYGPFGTGKSFILGALANELANLRIRTVVVYLPEFLREMKQSIQDQTLN
                     EKIDFVKRAKVLMLDDFGAESMSAWARDEVIGTILQYRMAEKLPTFFTSNFNFDELQH
                     HLTYTQRGEKEEVKAARIMERIKTIAKPVQLKGKNRRNM"
     gene            complement(2963..>3823)
                     /locus_tag="VFX34_05745"
     CDS             complement(2963..>3823)
                     /locus_tag="VFX34_05745"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEX5564441.1"
                     /translation="SEGMEEGGQMAGRNETSGIPFSYSDFDFHLFRAGLSEQMVPKSA
                     LASVSNKLIEKLAFLYSLTPLDMQKVVMMALDGNTMKIQEDRLRKAAVDYYKMNISQT
                     APSIEKVFTKQPAMPIEEPKSREDKFIFYMENTTPREMLKDYTGKEPLPVDVELAERL
                     VTVHGFPVGVVNVLLHYIIIRNDGKITKNYAERIASHWANNKIATAKQAMEISRKEHD
                     QYIKWQNEGSKKKPASRKPTRVEKVPEWFYKTDDEKEQQPAVDANPAIDEKRRQQLLE
                     KLDAMRSGVE"
BASE COUNT          890 a          923 c          830 g         1180 t
ORIGIN      
        1 agggatcact tcgacttgga ccggtgcaag ccatgtcggg aatgcacctt tgtattcttc
       61 gatcaggaat gcgacgaaac gttccattgt ggatacgact ccgcggtgga tgacgaccgg
      121 gcgatggtgc ttgccgtctt cgcctacgta agtgagatcg aaacgttctg gaagcaggaa
      181 gtccaactgg acagttgaaa gtgtctcttc catgccgatt gccgttttca cttgcacgtc
      241 aagtttcggg ccgtagaatg cagcttcgcc gtccgcttcg acataaggaa ggccaagttc
      301 gtccatagtc tctttcaaca tgctttgtgc gcgttcccac atttcgtcat cgtcgaagta
      361 tttttcagta tcttccggat cgcgatacga gagacggaac gagtagtctt caatattgaa
      421 gtctttatag acatcgatga cgagttgaac gacacgtttg aattcttcct tgatttgatc
      481 aggacggaca aagatatggg cgtcattcaa cgtcatcccg cggacacgct gcagaccgga
      541 taaagcaccc gacatttcat agcggtgcat cgttccgagc tccgcgatgc ggatcggcaa
      601 gtttcgataa gaatgaatgc catttttgta aatcatcata tggtgcgggc agttcatcgg
      661 acggagaacg agatcttcat tatccatgct catgacaggg aacatgccgt cctggtaatg
      721 cccccagtga cctgatgtct tgtaaagttc aacgcttccg agtaccggtg tatagacatg
      781 gtcatagccg agcttcactt ctttatcgac gatataacgt tcgatgatac ggcggatcgt
      841 agcacctttc ggcaaccaaa gcggaaggcc ttggcctact ttctgggagt tcgtgaacaa
      901 attcaattcc ttcccgattt tacggtgatc gcgttccttc gcctcttcga gcatccggag
      961 gtgttcctga agttcgtcct ttttaaagaa tgctgtgccg taaatccgtt gaagcatttt
     1021 gtttttcgag tcgccacgcc aatatgcgcc tgcgatgctc aataatttga attcttttag
     1081 tttgcctgtc gaaggtacat ggatgcctcg gcaaaggtcg aagaattcgc cttgctcata
     1141 gatggacact tgctcgcctt ctggaatggc ttccaacagt tcaagtttgt atggatcttc
     1201 gatttccttg aaacgctttt cggcttcttc acgggacaca tcgattcgtg tgatcggaag
     1261 gttttcaccg atgatccgtt tcatttcttt ctcaagttct ggaagatctt cagctgtgat
     1321 tggcgtcggc gaatcgatat catagtaaaa tccgttgtcg atgaccggtc caattcctaa
     1381 tttagcgtcc ggaaatttac gttttactgc ctgcgcaagc aaatgggctg tactatgacg
     1441 taggatttca agcgcttctg gtgattgtgg tgtgatgatt gcaatttcac catcattttc
     1501 aatcggtgtt ttcaagtcga tgagctggtc accgatttta ccggctaatg cactttttcg
     1561 aagtcctgga ctgatggatc ctgccacttc ttctgttgta gtgcctttcg gaaactcttt
     1621 tatcgcgcca tccggaaatt tcaattgaat attttctgcc atgataaaca ctcctcactt
     1681 atttttttga acaaaaaaag ccccatccct ataaagggac gaggctgttg ctcgtggttc
     1741 cacccttctt ccttccatcc agtcattcaa tgaaaactgg aaagatgaag ctcgaaacgg
     1801 ctaacgggcc gggtccgtca tcggttacta aaagggttcg ccgatgctgc tccaaggtgg
     1861 taaatcgatc ctctgcactt cagggcttcc agccaaatgg ccccgatctc tgtaaagtgg
     1921 atgaaccgat ccatgtcctc ttcaatacat tttcaaatat aatagttatt agtataagga
     1981 aagtctcaga taattgcaag tggttaaatt cacttacata ttcctgcggt ttttcccttt
     2041 taattgcaca ggctttgcga ttgtcttaat ccgttccata atccgcgctg ctttgacctc
     2101 ttctttttcg ccccgctgtg tatatgtcaa atgatgctgc agttcatcga agttgaaatt
     2161 cgatgtaaag aatgtcggaa gtttctccgc catccggtat tgcaatatcg tcccgatcac
     2221 ttcgtcgcgc gcccatgcgg acatggactc cgcaccaaag tcatcaagca tgagtacttt
     2281 agctcttttt acgaaatcga ttttttcatt caacgtctga tcttggattg attgcttcat
     2341 ttccctcagg aattcaggga gataaacgac aactgttcgg attcgaagat tcgcaagctc
     2401 attcgccagc gcaccaagaa taaaggactt ccctgttccg aacggcccgt aaatataaag
     2461 cccccgttct ggcagctcgc ctgttttcct tatctcactc aggaattgct cagctgcttc
     2521 aacgataatg tatctgctgt cgttatgctg cagatcatct acatcgtgca gtcgcgcctc
     2581 cataacgtct ttcggcatat acatgctgtc gatcatattg gatacgttac gtctttcatc
     2641 atcgacgagc ttattcgggc atttgatata gtcgaggtcg atgaaccctt gagcaagaac
     2701 gagcttaggt tcaaatcctt tcatcacatt gatgcaccct tcgatattcg gacatttctc
     2761 gcaatggtgg gaggctgtcg cgtattcata taatttcccg aggcttttgt caacgaccgc
     2821 tttatcaatt tcattcgaat gctcggagat gaattgttgg acgccaggcg attccatcac
     2881 ttcattccgt aattgctcgt atcttgctgt tagatccgaa gcgttgaata cacgcttcat
     2941 tgtatttcca attcgctcca acttactcca ccccacttct cattgcatcc agtttttcta
     3001 gcaactgctg acgacgtttt tcatcaatag ccggatttgc atcaactgcc ggctgctgtt
     3061 ccttttcatc atctgtttta taaaaccact cgggcacctt ttccactctc gtcggctttc
     3121 gggatgccgg tttcttctta cttccttcat tttgccattt aatatattgg tcatgttctt
     3181 tgcgggatat ttccattgcc tgttttgccg tcgcaatttt attattcgcc caatgcgagg
     3241 cgattctttc cgcatagttt tttgtgattt taccgtcatt gcgaataatt atatagtgca
     3301 gcagtacatt taccacgcca actgggaagc cgtgaacagt tacaagtctt tcggcaagtt
     3361 caacgtctac aggaagcggt tcttttccag tatagtcttt cagcatttcc cgaggggtag
     3421 tattttccat atagaaaatg aatttatctt cccgtgattt aggttcttcg attggcatgg
     3481 ccggttgttt cgtgaagact ttttcgatgg atggagcggt ttgtgaaata ttcatcttat
     3541 aataatccac agccgctttt cgcaatcggt cttcctgaat cttcatggtg tttccatcaa
     3601 gcgccatcat gacgactttt tgcatatcaa gtggggtcaa tgaatagagg aaagccaatt
     3661 tctcaatcaa tttattcgaa acagaggcga gcgcggattt tggcaccatc tgctcagata
     3721 accccgcacg gaacaaatgg aaatcgaaat cggaataaga gaacgggata ccgcttgttt
     3781 cattgcggcc agccatttgc ccaccttctt ccattccttc cga
//
LOCUS       DASULI010000267         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_217260, whole
            genome shotgun sequence.
ACCESSION   DASULI010000267 DASULI010000000
VERSION     DASULI010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217260"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..334
                     /locus_tag="VFX34_05750"
     CDS             11..334
                     /locus_tag="VFX34_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX5564442.1"
                     /translation="MNIQFKKGVLNLCVLVLLDKQDRYGYELVQKISDQISISEGSVY
                     PLLRRLTKEGYFTTYLQESTEGPPRKYYKLTDAGRDYLHEQLDEWRSFTDGVNTLIEE
                     GVRHD"
     gene            327..1373
                     /locus_tag="VFX34_05755"
     CDS             327..1373
                     /locus_tag="VFX34_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX5564443.1"
                     /translation="MTENQFINELENALKRLPTDERNDILQDIREYFSNGREDGKAEE
                     EIAASLGSPAKIASDLLDAYPFEDLEVSQGTRLSTSPSSEVVTIQDDSFTKVDIEVFH
                     GKLSVFPSESSETKIELINVNDKLEFSADVLEDTLRVRLKKKGLWLFIFNFNTKEVAL
                     NVFIPKKLYQSISMKSDNGRINAEKLIGKNIEARTDNGRIELTELAATSLHAETDNGR
                     IEISKVQTERLKAKTNNGRVTMRHVEADSIYAESDNGRIEFDQVDGEITAITDNGRIV
                     LAGEHLDRNIDFQTDNGSIEITTTQKATNATILAKTGHGRIDVYGERNSRSRFGAELH
                     QIRLKSDNGRITVR"
     gene            complement(1407..1640)
                     /locus_tag="VFX34_05760"
     CDS             complement(1407..1640)
                     /locus_tag="VFX34_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564444.1"
                     /translation="MKKEEEVRMTIEESERYGRAINARNAAVERNQVYEEEEMIPADQ
                     LPPPLSEDEIKKRVGETNYNYMKDDNVMNSINS"
     gene            1790..2242
                     /locus_tag="VFX34_05765"
     CDS             1790..2242
                     /locus_tag="VFX34_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEX5564445.1"
                     /translation="MVMIFVDADGCPVVNETISIAKQFNLPCTLICDTAHEMHREGAE
                     TIIVSKGADAVDFVLVNKVQKGDIVVTQDYGLAAMVLAKQGHPIDQNGRLYTNENIDQ
                     LLFARHAAQKIRMAGGRLRGPKKRSKESNDKFKEKLRHLCKMLLSPSE"
     gene            2394..>3327
                     /locus_tag="VFX34_05770"
     CDS             2394..>3327
                     /locus_tag="VFX34_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX5564446.1"
                     /translation="MPKILSVSTVLPPHEVKQAEAVELTRSLFSRKFKDIERLLNVFQ
                     NGDIEKRDVCMPLEWYGQAHDFEERNDLYIRHAIDFGVQAVQACLQQSATLAEAVDTS
                     EIDAIFFISSSGIATPSIEARIMNRLPFRDDVKRVPIWGLGCAGGAAGVSRAFEYCKA
                     FPKANVLVLSIELCSLTFQKDDYSKSNLVGVSLFSDGVACALISGDQSEIAVDIPVPS
                     IIGTTSKWMPDSEDVMGWDIKSNGLYVIFSKSIPSIITNWLGPFVHEFLDSYNLGKND
                     ITHFVAHPGGKKVLTAYEDSLGFDSSMTDISRNVL"
BASE COUNT          943 a          725 c          769 g          890 t
ORIGIN      
        1 agggctagtg gtgaatattc aattcaaaaa aggcgtatta aatttgtgcg tccttgttct
       61 attggataaa caagatcgct atggttatga attggtacaa aaaatctccg accagatatc
      121 catttctgaa ggatctgttt atccactttt gcgccggtta acaaaggaag gctattttac
      181 gacctattta caggaatcga cggaagggcc tcctcgaaag tattacaagc tgaccgatgc
      241 cggacgagat tatttgcatg aacagttgga tgaatggcgc agttttacag atggggttaa
      301 tacattaatc gaggagggcg ttcgccatga ctgaaaacca atttatcaat gagcttgaaa
      361 acgctttgaa acgtttgcct actgatgaaa gaaacgatat tctccaagat atccgcgaat
      421 atttttcaaa tggccgggag gatgggaaag ctgaagaaga aattgcagct tctcttggtt
      481 ccccagcgaa aattgcatct gatcttttag atgcgtatcc atttgaagac cttgaagtat
      541 cacaggggac ccgtttgtca acatcccctt caagtgaagt ggtaaccatc caagacgaca
      601 gcttcaccaa ggtggatatc gaagtttttc atgggaagct ttccgtattt ccttcagaga
      661 gctctgagac aaaaattgaa ctgatcaacg tcaatgacaa actggaattt tctgcagacg
      721 ttcttgaaga tacgttgcgg gtcagattga aaaagaaagg actttggtta ttcatcttca
      781 atttcaatac gaaggaggtt gcattgaacg tgttcatccc taaaaagctc tatcaatcca
      841 tttcaatgaa atccgataat ggccgcatca atgctgagaa gttgattggt aaaaatatcg
      901 aagcgcgcac ggataacggc cggattgaat tgactgaatt ggcagccact tcacttcatg
      961 cagagaccga taacggaaga atagaaatct cgaaggttca gactgaacgg ttgaaggcga
     1021 aaacgaataa tggccgggtg acgatgagac atgtcgaagc cgattcaatc tacgcggaat
     1081 cggacaatgg ccgtatcgag ttcgaccaag tcgatggtga gattactgca attactgaca
     1141 atggccgtat tgtcctcgcc ggagaacatt tggatcgcaa catcgatttc cagacggata
     1201 acggcagcat tgaaattacg acaacacaga aggcaaccaa cgctaccatt ttagcgaaaa
     1261 ccggacacgg acggatcgat gtttacggcg aacggaattc acgcagccgg ttcggcgccg
     1321 aattgcacca aatccgtttg aaatcagata acggaaggat aacggttaga taagtgcaca
     1381 aaaaagaggg cgatcatccg ccctctttaa ctgtttatgg aattcatgac gttatcgtct
     1441 ttcatatagt tgtaattcgt ttcaccgacg cgctttttga tttcatcctc gctcaatgga
     1501 ggcggaagtt gatcagccgg aatcatctct tcctcttcat atacttgatt cctctcgacc
     1561 gccgcattcc gggcgttaat cgctcggcca tatcgttcgg actcctcgat cgtcatcctt
     1621 acttcttctt cctttttcat gcctgcttcg ctcctttaac atatttacaa tcaaataccc
     1681 ttgttgaatg aaaagaaaac gaatgaatat gatatagtga ggcatcaatc catgtatagt
     1741 atgattatag aaaatcgctt tcatacacca attgaaaaga ggtcttgcca tggttatgat
     1801 ttttgtggat gcagacggct gtccagtcgt aaatgaaacg atttccattg caaagcaatt
     1861 caatctgcct tgcacgctca tttgcgacac ggcccatgaa atgcatcgtg aaggggcaga
     1921 gacgatcatc gtttccaaag gggcagatgc tgtcgatttt gttcttgtca ataaagttca
     1981 aaaaggagat atcgttgtta cgcaggacta tggattagct gccatggtgt tggcgaagca
     2041 agggcacccg atcgatcaga acggcaggct gtatacgaac gaaaatattg accagctctt
     2101 attcgccaga catgccgcac agaagatcag gatggcgggc ggacgtctgc gcgggcctaa
     2161 gaaacgttcg aaagaaagca atgacaaatt taaagaaaaa ctacgtcatt tgtgcaaaat
     2221 gctactttct ccatccgaat gaagcccgct cttttatcgg gctttcatat tcgttccccc
     2281 ctattcataa actgctgtaa gtgcaactcc catttttcac ctttcaaaat tcaacgatgt
     2341 ttagtaactg actgcaaagg ttagagatta ataaagatat cggaggtcta tccatgccaa
     2401 aaatcctttc cgtcagcacc gtcctgcctc cccatgaggt gaagcaggcg gaagctgttg
     2461 aattgacccg ctctcttttc agccgtaaat tcaaagatat tgaacgactt ttgaatgtct
     2521 ttcaaaacgg cgatattgaa aagcgggatg tttgcatgcc acttgagtgg tatgggcaag
     2581 cccacgattt tgaagagcgg aacgacctgt atatccggca cgcgatcgat ttcggtgttc
     2641 aggctgtaca agcatgcctt caacaatccg ctacattggc tgaagcagtg gatacatcgg
     2701 aaattgatgc aattttcttc atttcaagtt caggcattgc tacaccaagc atagaggccc
     2761 gcatcatgaa cagactccct ttccgtgatg acgtgaagcg tgttccaatc tggggattag
     2821 gctgtgcggg aggggcggcg ggagtcagcc gggcattcga atattgtaaa gcttttccga
     2881 aagcgaatgt gttggtgttg tccatcgagc tttgcagttt gacgttccaa aaggacgact
     2941 attcgaaaag caatcttgtc ggcgtatcct tgttctcgga cggcgtcgcc tgtgcgctta
     3001 tttcagggga tcaatccgaa attgctgtcg acatccccgt cccgtcaatt atcggtacga
     3061 cttcgaaatg gatgcccgac tcagaggacg tcatgggatg ggacattaaa agcaatggtc
     3121 tctatgtcat cttctcgaaa agcatcccat ccattatcac caattggctc gggccattcg
     3181 tgcatgaatt ccttgattcc tataatcttg gaaagaacga tatcacccat ttcgttgcgc
     3241 atcccggtgg caaaaaagta ttgacggcgt atgaggattc attaggattt gacagctcaa
     3301 tgaccgatat ttcaaggaat gtgctgc
//
LOCUS       DASULI010000268         2225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_72768, whole
            genome shotgun sequence.
ACCESSION   DASULI010000268 DASULI010000000
VERSION     DASULI010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72768"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..862)
                     /locus_tag="VFX34_05775"
     CDS             complement(17..862)
                     /locus_tag="VFX34_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5564447.1"
                     /translation="MKEILSLDHVSYSYESEDGESRRAVDDVSFTVNDGEWIAIVGHN
                     GSGKSTLAKLMIGLLFPEEGKVKVFLEELNAENLWEIRSDIGIVFQNPDNQFVGSTVQ
                     DDVAFALENNGIPFEEMVPRVHESLAQVKMEGFLDHEPHHLSGGQKQRVAIAGALAMR
                     PKLLILDEATSMLDPQGRDEVIQVVDELKRRTGLTVISITHDLEEVLLADHVMVMNKG
                     KLMLTGKPGEIFAHGDELEAIGLDLPFAARLSRSLRQAGVAIQGEHMTEEELVNELWT
                     SHFNK"
     gene            complement(1155..1535)
                     /gene="rplQ"
                     /locus_tag="VFX34_05780"
     CDS             complement(1155..1535)
                     /gene="rplQ"
                     /locus_tag="VFX34_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5564448.1"
                     /translation="MGYRKLGRTSSQRKAMLRDLATDLIVHERIQTTEARAKELRSVV
                     EKMITLGKRGDLHARRQAAQFIRRELVTTADEEGNEKEIFALQKLFDNVAPRYADRQG
                     GYTRIMKMGPRRGDGAPVVVIELV"
     gene            complement(1564..>2225)
                     /gene="rpoA"
                     /locus_tag="VFX34_05785"
     CDS             complement(1564..>2225)
                     /gene="rpoA"
                     /locus_tag="VFX34_05785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5564449.1"
                     /translation="VIEIDVKGEGTVTAADITHDSDVEVLNPDLQIATLGKNGHLRMR
                     MYAVRGRGYAPSDQNKREDLAIGVIPIDSIYTPVSRVNFQVENTRVGQSTNFDKLTFD
                     IWTDGSIGPKEAVSLGAKILTEHLNIFVGMTDEAQTAEIMVEKEEDQKEKVLEMTIEE
                     LDLSVRSYNCLKRAGINTVLELANKSEEEMMKVRNLGRKSLEEVKAKLDELNLGLRSE
                     D"
BASE COUNT          540 a          588 c          456 g          641 t
ORIGIN      
        1 tttgcgtatg aatagcctac ttgttgaagt gagatgtcca taattcattc accaactctt
       61 cttccgtcat atgttctccc tgtatcgcga cacctgcttg gcgaagggag cgcgacaatc
      121 tagccgcgaa aggcaaatcc agtccgattg cctccagctc gtccccatgt gcgaaaattt
      181 ctcctggctt gccggtcaac atcaactttc ccttattcat gaccataaca tgatccgcca
      241 acagcacttc ttccagatca tgcgtaatcg agatgactgt cagccctgtt ctccgcttca
      301 gttcatcgac tacttggatc acttcatcgc gcccttgcgg atcaagcatc gatgtcgcct
      361 cgtccaaaat gagcagcttc gggcgcattg caagcgctcc tgcaatggct acacgctgtt
      421 tttgtccgcc ggatagatga tgaggttcat gatccagaaa tccttccatt ttcacttgtg
      481 cgagtgattc gtggacacgc ggcaccatct cctcgaaagg aatgccgttg ttctccaaag
      541 cgaatgcaac atcgtcctgg acggtcgagc cgacgaactg attatccgga ttttgaaaga
      601 cgattccgat gtccgaacgg atttcccaaa ggttttccgc attcagctct tccaggaaga
      661 ctttcacctt tccctcttca gggaataaaa gaccgatcat cagcttcgct aacgtcgatt
      721 tgcctgaacc gttatgaccg acaatggcaa tccattcacc gtcattgaca gtgaaactga
      781 catcatcaac tgccctgcgc gattcaccat cttccgactc ataggaatag gacacatgat
      841 ccaatgacaa aatttccttc acatctttca cctccgggtt tttctgatta tgtatgaaac
      901 gattgaattc tacaattttt tctcggcata aataaaaaaa gcgcgcacct catccgaaga
      961 aatgcgctcc attctattct gatgccgggt gctcaatccg gatcagcgga tgagatgcgt
     1021 agagctagac cgatcggccg aaccgtcgat cataacactc agcttttaat attgtcgtat
     1081 aaatcgttta gataagaaaa gggcgtggct gtgcgtatgc cagacacacc ctcgattgtt
     1141 gatctattta ttgattacac aagctcaata actacaacag gtgctccgtc tccacgacgg
     1201 ggtcccattt tcatgatgcg agtgtagccg ccttggcgat cagcgtagcg tggtgccacg
     1261 ttatcgaaca atttttgaag tgcaaaaatc tctttttcgt tgccttcttc atccgcagtt
     1321 gtcactagct cacgacgaat gaattgtgcc gcttgacgac gtgcatgcaa gtcaccgcgt
     1381 ttaccaagcg tgatcatctt ttcaacgact gaacgtaatt ctttcgcacg cgcttctgtc
     1441 gtttggatgc gctcatgcac gattaggtct gtcgctaagt cgcgaagcat tgctttacgt
     1501 tgtgaacttg tacgtccaag ttttctgtaa cccatagaag tttccctcct tcaatctagt
     1561 tgattaatct tccgagcgta aaccgaggtt caactcgtcc aatttcgcct tcacttcttc
     1621 aagtgattta cggccaaggt tgcgcacttt catcatttct tcctcggact tgttggcaag
     1681 ttcaagtaca gtgttgatgc ctgcgcgttt caggcagtta taagatcgca cagatagatc
     1741 aagctcttcg atagtcatct caagcacttt ttctttctgg tcttcctctt tttccaccat
     1801 gatttcagca gtttgtgctt cgtcagtcat gccaacgaaa atattcagat gctccgtcag
     1861 gatttttgct ccgagcgaaa cagcttcttt cggaccgatg ctgccatctg tccaaatgtc
     1921 aaacgtcaac ttatcaaagt tagtgctttg acctacacga gtattctcga cttggaaatt
     1981 gacgcgtgaa actggagtgt aaatagagtc gatcggaata acaccaatcg caagatcctc
     2041 acgtttgttt tgatcggaag gtgcataccc tcggccccga acagcataca tccgcatacg
     2101 caaatgacca ttcttgccca gtgtagcgat ttgaagatcc ggattcagca cttcaacatc
     2161 actgtcatgg gtgatatccg ctgcagtgac cgttccttca ccttttacat ctatttcaat
     2221 cactt
//
LOCUS       DASULI010000269         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1907554,
            whole genome shotgun sequence.
ACCESSION   DASULI010000269 DASULI010000000
VERSION     DASULI010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907554"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFX34_05790"
     CDS             complement(<1..289)
                     /locus_tag="VFX34_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX5564450.1"
                     /translation="MIIGLFLLVVLSFIHLTQGQADYTVSQLLKEVWVEGRIQDIVLS
                     LRLPRLAIGILAGGTLAVAGAILQTLTNNPLASASTLGINAGAYFFVVVSMI"
     gene            complement(328..1314)
                     /locus_tag="VFX34_05795"
     CDS             complement(328..1314)
                     /locus_tag="VFX34_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5564451.1"
                     /translation="MNRYMKALLLLALSVILVAGCSSKKTDEKGAGGETTENGVTIVG
                     TNGEVKLDKPAKKVVVLEWTYAEDLLALGVQPAGMADIQEYHNWVNIDAELSDDVVDV
                     GGRQEPNLEAIAAIEPDLIIGVSFRHDAMLKDLEKIAPTVIFNPYPEDESIDLYKEMT
                     TTFNEIAKAVDKKDEAEKVLAHLDEKYEEAKAEIEKADLKTKDVILTLAYTGPQAPEI
                     RVFTPNSMASQILEKIGLKNVHVPDQFEVFGSSTFNVEGLTKYEDANYLYTVQEEDNV
                     YEKQLKDNAVWKNLNFVKEDRLYNLGSDTWLYGGPLSAETLLNRIVDTMVNK"
     gene            1490..2314
                     /locus_tag="VFX34_05800"
     CDS             1490..2314
                     /locus_tag="VFX34_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564452.1"
                     /translation="MHPTINTKNLSIGYEDHLLFKNLNLAIPRGEITVFVGSNGCGKS
                     TLLRSIARLLKPTDGSVLLEGKDIHSMSSRNVAKKMGILPQGPVSPEGLTVHDLVKQG
                     RYPHQSWLSRWTAEDTKKVEAAMEATKISDLRDQAIDTLSGGQRQRAWIAMTLAQDTD
                     VILLDEPTTYLDMTHQIEILDLLFELNEQKGRTIVMVLHDLNLASRYAHNIVAIKDGG
                     VYAQGRPENIITCDLVRAVFGMECQVSTDPLFGTPHCVPYGRGRCIVQEVRSNTGA"
BASE COUNT          596 a          616 c          500 g          631 t
ORIGIN      
        1 aaatcatcga cacaacgaca aagaaatacg cgccggcatt gatgccgagc gtacttgcgg
       61 acgccaacgg attattcgtc aacgtctgca agatggcacc cgcaacagcg agcgtacctc
      121 ccgctaaaat accaattgcc agacgaggta gccggaggga cagcacgata tcctggatgc
      181 gcccctccac ccaaacttcc ttcaatagct gggaaactgt gtaatcagct tggccttgcg
      241 tcagatggat gaaggataaa acgaccaata ggaaaagacc gatgatcata acgaacaacg
      301 atcttttccc tgaagccttt cccgccatta tttattgacc atcgtatcga cgatccggtt
      361 caacaatgtt tcggctgaca gcggacctcc atacaaccaa gtgtccgaac cgaggttata
      421 tagtcgatct tccttgacaa aattcaagtt tttccaaact gcattatctt tcaattgctt
      481 ttcgtataca ttgtcttctt cctgcaccgt atacaaatag ttcgcatcct cgtatttcgt
      541 caacccttcg acgttgaatg tgctcgaccc gaatacttca aattgatccg gtacgtgtac
      601 gtttttcaag ccgatctttt cgaggatttg ggaagccatt gaattcggcg taaatacccg
      661 gatttctgga gcttgcggac ctgtataagc gagcgtcaag atgacgtcct tcgtctttaa
      721 gtcggccttt tcgatttcag ctttcgcttc ctcatatttc tcatccaagt gcgccaacac
      781 tttttcagct tcatccttct tgtctacagc tttcgcgatt tcattgaagg tcgtcgtcat
      841 ttccttgtac aaatcgatgg actcgtcctc cggatatgga ttgaagatga ctgtcggagc
      901 gatcttctcc aaatccttca gcatcgcatc gtgacggaag ctgacaccga tgatcaaatc
      961 cggctcaatt gcagcgattg cttccaaatt cggctcttgc cgtccgccga cgtcgactac
     1021 gtcatcactc aactccgcat cgatattcac ccaattgtga tattcctgga tatctgccat
     1081 acctgctggc tgcacgccaa gagcaagcag atcttccgcg tacgtccact cgagtacaac
     1141 gactttcttc gcaggcttat caagcttcac ttcaccgttc gtgccgacga ttgtcactcc
     1201 attttcagtt gtttcgccgc ctgcaccctt ctcatctgtt ttcttcgatg aacaaccggc
     1261 tactaaaatt acacttagag ccagcaacaa caatgctttc atatatctat tcatgtttta
     1321 cctccacgtt gataatcatt atcagttagt agtgtaatct tttagaccat catttgtaaa
     1381 gtgttttcaa caaatcaatg attatcattc tcaattggtt gaaaatatga tatgcttctt
     1441 agtattgagt tcattccttt taggataaat gaaacggagg cattctagta tgcaccctac
     1501 tataaatacg aaaaacctct ccatcggcta tgaagatcat cttcttttca agaatttgaa
     1561 tttggcgatt ccccgcggtg agatcaccgt ctttgtcgga agcaacggct gcggaaaatc
     1621 cacgttactc cgttcgatcg cccgtttgtt gaagcctacg gacggctccg tcctactgga
     1681 agggaaagat atccattcca tgtcttcccg caatgtcgcg aaaaagatgg gcatcctccc
     1741 ccaaggtccc gtctcacctg aagggttgac ggttcacgac ctcgttaaac aaggccgcta
     1801 ccctcaccaa tcgtggcttt cccgctggac ggcggaagat acgaaaaaag tcgaggcagc
     1861 gatggaagca acgaaaatta gcgaccttcg cgaccaggca atcgatacat tatcaggcgg
     1921 acagcgccag cgtgcgtgga ttgcgatgac acttgcccag gacacggacg tcattctgtt
     1981 ggatgagcct acgacatatc ttgacatgac gcaccaaatc gaaattttgg acctcttgtt
     2041 tgagttgaat gaacaaaagg gccgcacaat cgtcatggtg ctccatgatc tgaatttggc
     2101 ttcccgttat gcccataata ttgtcgccat taaagacggc ggagtttatg cgcaaggcag
     2161 accggaaaac atcatcactt gcgacctcgt ccgtgcggtg ttcggcatgg aatgccaagt
     2221 gtcaacggat cccttattcg gaacgccgca ctgcgttcca tacggtcgtg gacgctgcat
     2281 cgtgcaggaa gtaaggagca atacaggtgc ctaagctgaa tgaagagcat atcgcctcgt
     2341 tag
//
LOCUS       DASULI010000270         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1929134,
            whole genome shotgun sequence.
ACCESSION   DASULI010000270 DASULI010000000
VERSION     DASULI010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929134"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1494
                     /locus_tag="VFX34_05805"
     CDS             253..1494
                     /locus_tag="VFX34_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX5564453.1"
                     /translation="MKATGIVVEYNPLHNGHVYHAQQARKATGADVIIAVMSGNFLQR
                     GEPAFVDKWARTKMALAAGVDIVFELPYRFATGNAPVFAQGAIGLLDAAGCGAFCFGS
                     EDGNIEPFQNSLRLVDRHADEYETTVKHAVAEGLSYPMALNKAYKTILAGQSAVPVAD
                     LSLPNNILGFHYMEASQSIGSSMDAVTIKRMGAGYHDETLDSQKIASATGIRKSFFGS
                     KTLDDVADFIPEATRNLLLEWQSERKGFANWESFYPFLRFIILRNGPSRLRSIADVSE
                     GIENLVFRAAQSQDSFQSFMDAVKSKRYTWTRIQRMLTHILTGFTKGMRTEMEKPAYL
                     RLLGMTSEGRSYLSERKKNLPLPLISKVASFSDPSLDMDITASNIYGLALAKGSVESK
                     IGLDYLLPPIMEDHFASNLFK"
     gene            complement(1467..2513)
                     /locus_tag="VFX34_05810"
     CDS             complement(1467..2513)
                     /locus_tag="VFX34_05810"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HEX5564454.1"
                     /translation="MKLKRIGLYAVALVLLLFLFIYPLDAYISKPGGAYDLAPIVEVE
                     GGDKDDVGTFNLMTISIGKATPASYVASKFSDRMKILPIYKVRREGENDKEYNIRQKK
                     LMSDSQFNAITVAFSKAGIPVDITYDGVFVISVLKGGASDGKLEAGDKIRSVDGVQLK
                     EAGQFASSITGKGEGDKVQVTVERDKKLLDIEITLREIPESQGRIGLGVEFQEDRELA
                     TDPKVDIHTADIGGPSAGLMFTLEIMNQLLDEDLTKGYNVAGTGEMLADGTVGRIGGA
                     DFKIMAADREGVEIFFAPNDYLPDEVRAKNPGIMTNYEEAVKTAEKIGTKMKIVPVTS
                     IDDALDYLNKLEAK"
BASE COUNT          713 a          655 c          590 g          756 t
ORIGIN      
        1 aatgctcctt gtctgtattt ctgtagttga tgaatggacc atttcacgtc tatatcacct
       61 caagacaaca ctgttgatta tataatggat aaaaagggat gtcaagatat tttcttgtca
      121 cctaaatcgc atcatcgtat aatgtaaatt aatgaatttc cgctgcggat ggacacattc
      181 cattctaatt caatgcagct tccaccctaa tttttattat actaaataaa gtataacgaa
      241 ggagcaatca taatgaaagc tacaggtatc gtcgtagaat ataatccatt acataatggg
      301 catgtctacc atgcgcagca ggctagaaag gcgactggcg cggatgtcat tattgcagtt
      361 atgagcggta atttcctgca aaggggcgaa ccggcctttg tcgataaatg ggcgaggacc
      421 aagatggcgc tagcagccgg agtagatatc gtgttcgaac tgccatatcg ttttgcaaca
      481 gggaatgccc cagtatttgc acaaggggct atcggcttgt tggatgcagc cggatgcggt
      541 gctttttgtt tcggcagtga ggacggcaat atcgagccgt ttcaaaatag cttacggctg
      601 gtcgaccgcc atgcagatga atatgaaacc acggtgaaac atgcagttgc tgaaggtcta
      661 agctatccaa tggcgttgaa taaagcgtat aagacgatac ttgctggaca atccgcagtt
      721 cctgtcgccg acttgtcgct gccgaataat attttagggt ttcattatat ggaagcttca
      781 cagtcgatcg gttcatcgat ggatgccgta acgatcaagc gaatgggagc cggctaccat
      841 gatgaaacgt tggatagtca gaaaatcgca agtgcaacag gcatccgtaa atcctttttc
      901 gggtcgaaaa cactcgatga tgttgctgac ttcattccag aagccacccg gaacctcctg
      961 cttgaatggc aatcggaaag aaagggattc gccaattggg aaagttttta cccatttctc
     1021 cgattcatca ttttgcggaa cggtccttcc aggctacgca gcattgccga tgtttccgaa
     1081 ggaatcgaaa accttgtttt ccgtgcggca caatcccagg attcattcca atcgttcatg
     1141 gatgcagtta agtccaaacg ttacacttgg acgcggatac agcggatgct gacgcatata
     1201 ctgaccggtt tcacgaaagg tatgcgaacc gaaatggaaa aacccgctta tttgcgtttg
     1261 cttgggatga ccagtgaagg gcgttcctat ctaagcgaaa ggaaaaagaa tctgcctctg
     1321 ccgcttatta gcaaagtcgc atcattttcc gacccctcat tggacatgga tataaccgca
     1381 tctaatattt atggattggc ccttgcaaaa gggagtgttg aatctaaaat cggtttggat
     1441 tatctgcttc cgccgatcat ggaagatcac ttcgcctcca acttgttcaa ataatccaat
     1501 gcatcatcaa ttgacgtcac aggtacgatt ttcatcttcg tgcctatttt ttctgccgtt
     1561 tttactgctt cttcatagtt cgtcatgatg ccggggtttt tcgcccggac ttcatcgggc
     1621 aaataatcgt tgggagcgaa aaagatttcc actccttcgc ggtcggctgc catgattttg
     1681 aaatcggcac ctccaatgcg gccgactgtt ccatcggcaa gcatttcccc ggttcccgca
     1741 acgttgtatc cttttgtcaa atcctcgtcc aacagctgat tcataatttc caacgtgaac
     1801 ataagacccg cggacggccc tccgatatca gctgtgtgga tatcgacttt cggatcggtt
     1861 gccagttcgc ggtcctcctg gaattcgaca cctaaaccga ttcttccctg cgattccggt
     1921 atttcacgta aagtgatttc aatatccaag agcttcttat cccgttctac agtaacctgt
     1981 actttgtctc cttcaccttt cccagtgatt gacgatgcga attgcccggc ctctttcagt
     2041 tgtaccccat cgaccgaccg gattttatct cccgcctcaa gttttccatc cgatgccccg
     2101 cctttaagga cagatatgac aaagacacca tcatacgtaa tatcgacggg tatcccggct
     2161 ttgctgaatg ccactgtaat cgcattgaat tgggaatccg acataagttt tttctgccgg
     2221 atattatact ctttatcatt ttccccttcc cttctgacct tgtagatcgg caaaattttc
     2281 atccggtcag aaaattttga ggcgacgtag gaagcaggag tggcctttcc aattgaaatg
     2341 gtcatgagat tgaatgtacc cacgtcatcc ttatctccgc cctcgacttc cacaatcggt
     2401 gccagatcgt aagctccgcc cggttttgaa atgtatgcat cgagaggata aatgaataaa
     2461 aacaatagta aaactaaagc caccgcatac aatccgatcc ttttcaactt catatccaca
     2521 cacctccatt aatagcctgt acagttagca catatatatt acatagaaaa gcgtaggacg
     2581 cttgcgacta gtgctcgtaa cactccgctt ttactcgcaa aagctgtact ttgtgacagc
     2641 tttagctgcg acaggcataa ggcgaaggtg cgaagtggcg acctttgcca cacagcaact
     2701 ttgacttatg accc
//
LOCUS       DASULI010000271         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1428738,
            whole genome shotgun sequence.
ACCESSION   DASULI010000271 DASULI010000000
VERSION     DASULI010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428738"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFX34_05815"
     CDS             complement(<1..197)
                     /locus_tag="VFX34_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HEX5564455.1"
                     /translation="MINLNEYGWNQFHQENMDTIVEQLKAENYVAGRVTLEHKRMYRV
                     MTSEGEWLSVCSGAFQHAAHER"
     gene            complement(411..1016)
                     /locus_tag="VFX34_05820"
     CDS             complement(411..1016)
                     /locus_tag="VFX34_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5564456.1"
                     /translation="MSLISMYITNMLGYMIVAFPIYVIVRIVLLKRRKQPVNKLHELF
                     LALFVLYSVGIASQTIIPKWVMGVDGTTGKLFFDVYMQRDWVSTNAVPFKTISGYLQV
                     NEYLNGWDSIAISNLLGNIFLFSPFGFFVPLFWRRMDSFTAIVFMGLAVTCFIEGTQY
                     FIGRSTDIDDIMLNTIGVVIGYGVFLVWKVFAVNEKNVDHS"
     gene            complement(1193..>2135)
                     /locus_tag="VFX34_05825"
     CDS             complement(1193..>2135)
                     /locus_tag="VFX34_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5564457.1"
                     /translation="INGDLCEKGPDSLSTVAFARKLSEESERVFITKGNCDVVHDYVF
                     NNVEGIKNYMQQRKNTILNEMLDHHGKSLDDFATLRELGKYYRAYFGEVLDWLEQLPI
                     AYETDDFILIHAGVEDIPDWKMTSREFALYAPSFYEKGHQADKPVIVGHWPVVNYRPD
                     SVSSNCPLIDLEKKVIAMDGGNQIKKDGQLNALIIEGEGITYTYVDGLVEKTITRSHV
                     DETGRVGTVTYPNYELRILQEDDFFTLCENVKLGIKQWIKNEFIHEGNCKNDLSTTFL
                     SVEKGETVSIVDDCHDGYALVKSGTGEVGWIPKDCLV"
BASE COUNT          569 a          535 c          414 g          617 t
ORIGIN      
        1 cgttcgtggg ctgcatgctg gaaagcgccc gagcagacgg agagccattc cccttctgat
       61 gtcatgacac ggtacatccg tttatgttca agcgtcacac gtcctgcgac gtaattttcc
      121 gctttcagtt gttctactat cgtatccata ttttcttgat ggaattgatt ccacccgtat
      181 tcgtttaaat tgatcaatgt taaatcctcc tgttatgttc gtctgcaaat aaaaaagcat
      241 ggttccggat cggacaccat gctctcacgc gcataacaga atagacatgc cgattacgaa
      301 ggcatggcag gtgaaattat ggtgccgatc aattgtgcaa tagccgtatt gatgttcgcc
      361 ataaaacttc accccgcttt ctgaaagttg atttcatctt agcaaatcgc ttatgaatgg
      421 tcaacatttt tttcattcac agcaaatact ttccaaacga gaaagacgcc atacccgata
      481 acaacgccga ttgtattcaa catgatatcg tcaatatccg tgctgcggcc gatgaagtat
      541 tgggtgcctt caatgaagca agtgacagct aaccccatga atacgattgc tgtgaatgaa
      601 tccatcctcc gccaaaatag tggaacgaaa aatccgaacg gggagaataa gaatatattg
      661 cctaacagat ttgagattgc aatactgtcc catccattca agtattcatt cacctggaga
      721 taaccggata tcgtcttgaa cggaactgca ttggtgctga cccagtctct ttgcatgtat
      781 acatcgaaaa agagcttccc ggtagtaccg tcaactccca taacccattt ggggataatc
      841 gtctgggagg ctatccctac tgaatagagt acaaataggg caagaaatag ttcatgcaat
      901 ttattgacgg gttgcttcct tcttttgagg aggacgattc gcacaatcac ataaattggg
      961 aatgcaacga tcatataccc taacatattg gtgatataca tactaattaa cgacataact
     1021 tctccttttg actccgaatt ctgcttatag agcttgacgt tttcattcat gaagatgttc
     1081 cttttagtct acggaaagtt atcggtcatt tccggagagt tatcggtcat ttcatgactt
     1141 ttgactcata agaaaaagct tcgacaggca cctgccttcg aagcttttcg tattacacca
     1201 agcaatcttt cggaatccac cctacttctc cagtgccgga cttcactaat gcatatccat
     1261 catgacaatc atcgacgatt gaaacggttt cccctttttc aacggataag aacgtcgtgc
     1321 tcaagtcatt tttacaattg ccttcatgga tgaattcatt ttttatccac tgtttgatgc
     1381 caagctttac attctcgcac aatgtgaaaa aatcatcttc ttgaaggatg cgcaattcat
     1441 agttcgggta cgtgaccgtt ccgacacgcc cggtttcatc tacatgactc ctcgtaattg
     1501 tcttttcaac caatccgtcc acatatgtat aggtgatgcc ttcgccttca ataatcaacg
     1561 cgttcaactg tccgtccttt ttgatttgat tgccgccatc catcgcaatt acctttttct
     1621 ccagatcgat cagcggacag ttcgagctga cggaatccgg ccgataattg acaaccggcc
     1681 aatgcccaac gatgaccggc ttatccgcct gatgtccctt ttcgtaaaat gaaggggcat
     1741 acaatgcaaa ctcacggctc gtcatcttcc aatccggaat atcctccacc cctgcatgaa
     1801 tgaggatgaa atcatctgtt tcataggcga tcggcagctg ctccagccag tccagcacct
     1861 cgccgaaata tgcacgatag tattttccaa gttcccgaag cgtcgcgaaa tcgtccagcg
     1921 atttcccatg gtggtccagc atttcattca aaatggtgtt cttccgctgc tgcatgtagt
     1981 ttttgatgcc ctcgacatta ttgaaaacat aatcgtggac aacatcgcaa ttccctttcg
     2041 taatgaacac ccgttccgat tcctcactca gttttctcgc aaacgcaacc gtcgacaggc
     2101 tgtccggccc tttttcacaa aggtccccgt tgatg
//
LOCUS       DASULI010000272         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1742479,
            whole genome shotgun sequence.
ACCESSION   DASULI010000272 DASULI010000000
VERSION     DASULI010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742479"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..764)
                     /gene="folK"
                     /locus_tag="VFX34_05830"
     CDS             complement(249..764)
                     /gene="folK"
                     /locus_tag="VFX34_05830"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5564458.1"
                     /translation="MNTAYLSIGSNLGDRLAILQEAVRALDSNEKMDVTSVSAVYETE
                     PVGYTEQPNFLNIVVELQTSLDPFELLAACQSIEQSLGRVRDIRWGPRTADLDILLYN
                     NETIVSENLTVPHPRMHERAFVLIPLLELNPDVKHPVTGKHYREEPAAKEDGVVLYRG
                     FNGVASFWGMK"
     gene            complement(761..1129)
                     /gene="folB"
                     /locus_tag="VFX34_05835"
     CDS             complement(761..1129)
                     /gene="folB"
                     /locus_tag="VFX34_05835"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5564459.1"
                     /translation="MDFIHLNEMEFYGYHGALPEETRLGQRFRVTVSLGCDLSDAGKT
                     DDLSKTVNYAEIYSLCKRIVEGEPVKLIETVAESIAHEILFHYADKVVGCRVIVIKPD
                     PPIPGHYASVSVDITRGRMQ"
     gene            complement(1141..1989)
                     /gene="folP"
                     /locus_tag="VFX34_05840"
     CDS             complement(1141..1989)
                     /gene="folP"
                     /locus_tag="VFX34_05840"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5564460.1"
                     /translation="MELKFAQKAFRFGKTEIDFRQETVIMGILNVTPDSFSDGGKYSR
                     IDAALAHAEKMLRDGAKIIDVGGESTRPGHAPVSVEEELERVVPVVEALTRELGCVIS
                     VDTYKADVAEESLKAGAAILNDIWGAKHEPRIAEVAARYGAPIILMHNRDNTEYGEFW
                     PEVKRDLEESIRIAETAGVEPAHIWLDPGIGFGKTTEHNILMMQHLDKLSEMGHPVLL
                     GTSRKSMIGSVLNLPVSERLEGTGATVCYGIEKGCHIMRVHDVKEMTRMARMMDVLVG
                     KATYNG"
     gene            complement(1989..>2540)
                     /locus_tag="VFX34_05845"
     CDS             complement(1989..>2540)
                     /locus_tag="VFX34_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX5564461.1"
                     /translation="PTFYETPTVIVFQKQLPPTTRGHAKKAIWLETIRNSPEGDVRRK
                     SQNFLNSIKGRFELPSLKDTEGIFLNENGYVAEGVTSNVFWVTNGVLYTPSIRTGILP
                     GTTRAFLLTLAAKSGIPVKEGFYGKEDVKHADELFVTNAIQELIPITEINGVRFAGAE
                     GAMYRALHERYVNEVNKAKEGGN"
BASE COUNT          641 a          695 c          506 g          698 t
ORIGIN      
        1 cggaaaatat catccgctac tgtatctata gcatgcatct attgtacaca atttcatcaa
       61 acgggacaca ttttgcagta attttctatg tgagcagaaa aaaaccgcca cttaaatggc
      121 ggcaaagttg aattttttta acaacacgcg tacctaccac accttgctca tgtttcttcg
      181 catcattctc gaatgtgtgt aagcccgttt tctatcttct agacgtaccg gctgtatagc
      241 aatacgattt acttcattcc ccaaaaagaa gcgaccccat taaaacctct gtaaagaaca
      301 acgccgtcct ccttggcagc aggctcttct cgataatgct tgcctgtgac cggatgtttc
      361 acgtcagggt tcaattccag tagagggatg agaacaaaag ctcgttcatg catgcgagga
      421 tgcggaacgg tcaagttctc cgagacaatc gtttcattgt tatagagcaa aatatcaaga
      481 tcagcagtac gcgggcccca acgaatgtcc cgaacacgtc cgagtgactg ctcaatcgac
      541 tggcaagcgg ccaataattc gaacggatct aagctcgttt gaagctccac aacaatattt
      601 aagaaattcg gctgttctgt gtagcctacc ggttccgttt cataaaccgc tgaaacgcta
      661 gtgacgtcca tcttttcgtt tgaatcaagt gcccggaccg cttcctgcaa tatcgccagc
      721 cggtcaccaa ggttggaacc gatggataga tacgccgtat tcattgcatg cgccctctcg
      781 taatatcaac agagacggag gcgtaatgcc caggaatcgg cggatccggc ttaatgacaa
      841 ttacacggca accgactact ttatcagcat agtgaaagag aatttcatgc gcaattgatt
      901 ccgcgactgt ctcaatgagc tttacaggct ccccttcaac aatccgtttg cataatgagt
      961 aaatctccgc gtaattcacc gttttcgata ggtcgtccgt cttcccggca tccgaaaggt
     1021 cgcagccaag cgaaacggtc acgcgaaacc gttggccaag acgcgtttct tcaggcagtg
     1081 cgccgtgata gccgtaaaac tccatttcat taagatgaat aaaatccata cagatccccc
     1141 ttatccgttg taggttgcct tcccgaccaa cacatccatc atgcgtgcca tccgcgtcat
     1201 ctctttcaca tcatgaacgc gcataatatg gcagcctttc tctatcccat aacaaacggt
     1261 tgcacctgtt ccctctaaac gttccgaaac tggcaaatta agtacactcc caatcattga
     1321 tttacgggat gtgccaagaa gtactggatg gcccatttca gatagcttgt cgaggtgctg
     1381 catcataaga atgttatgct cggtcgtctt tccgaaaccg attcccggat ctaaccatat
     1441 atgtgcgggc tcgactcccg ccgtctccgc aatacggatg ctttcttcca gatcacgctt
     1501 aacttccggc caaaactctc cgtactctgt attgtctcga ttgtgcatta aaataatcgg
     1561 ggcaccgtaa cgcgctgcaa cttcagcgat tctcggttca tgctttgctc cccaaatatc
     1621 atttaggatc gccgctccgg ctttcaaaga ctcctcggca acgtccgctt tgtatgtatc
     1681 cactgaaata acacagccaa gttcgcgtgt aagtgcctcc acgacaggaa caacccgctc
     1741 cagttcctcc tctacggaaa ccggcgcatg tcccggtctt gtcgactccc cgccaacgtc
     1801 gataattttc gccccgtcgc gcagcatctt ctctgcatgc gcaagcgctg catcaatccg
     1861 gctatacttc cctccatcag agaaagaatc cggggttaca tttaaaatcc ccataatgac
     1921 cgtctcttgt cgaaaatcaa tctccgtctt tccaaaacga aacgcctttt gtgcaaattt
     1981 taactccatc agttgccacc ttcttttgct ttgtttactt catttacata tcgttcgtgc
     2041 agtgccctgt acatagcccc ttctgctccc gcaaaccgca caccattgat ttcagttatc
     2101 ggaattaatt cctgaatagc attcgttacg aagagctcat ccgcatgctt tacgtcttct
     2161 tttccgtaga atccttcttt cactggaatg cccgattttg cagcgagcgt aagtaaaaat
     2221 gcacgtgttg tccccggcag aatacccgta cggatcgatg gcgtatacag caccccattg
     2281 gttacccaga acacattgga tgtaacgcct tccgcaacgt agccattttc attcagaaag
     2341 attccctctg tatcctttaa tgaaggaagc tcaaatcgtc cctttatgct attcaaaaag
     2401 ttttgcgatt ttcttcgaac atctccttct gggctgttcc ttatcgtttc gagccaaatc
     2461 gctttcttcg catgtcctct tgtagtagga ggaagctgtt tttgaaacac gatgacagtt
     2521 ggtgtttcgt agaatgtcgg
//
LOCUS       DASULI010000273         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_606290, whole
            genome shotgun sequence.
ACCESSION   DASULI010000273 DASULI010000000
VERSION     DASULI010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606290"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1124)
                     /locus_tag="VFX34_05850"
     CDS             complement(6..1124)
                     /locus_tag="VFX34_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5564462.1"
                     /translation="MKKMSNLLFAILLFAGLMTTIGLSIKNAEAKKNPNTVYVYGPGG
                     PLGPMEELAKRYSKGKGIQVHVTGGPEGKWIEQAKKDGDIIYGGSSYMLQNFSFSHPE
                     LLDEESWVELYSRPAGILVRKGNPKNIHSMKDLAKDGVRIVDVNGAGQLGLWEDLAGR
                     ESLIPDIRKNIATSVKTSAEAIELWKSDSTIDAWITYESWHYRLQDVTDLVKLKDKLY
                     RGTPIALTEKGSKNKQAVKFLAFLQTEEAQEVFRKWGWKGPALQVLSHDKIDDYLADD
                     KGMTLYYFKNDVEGKSNCTGDCLANWPIVKAGEYEVPAGYDKADFGKITREDNGEEQL
                     TYKGYPLYYFTKDISQGDVNGEGVKDVWYVVNDETEFE"
     gene            1584..>2170
                     /locus_tag="VFX34_05855"
     CDS             1584..>2170
                     /locus_tag="VFX34_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5564463.1"
                     /translation="MMDYFLGLSPVYQALIATLFTWGMTAVGAALVFTTKKVNQKLMD
                     GMLGFAGGVMIAASFWSLLSPAIEMAEENSSFPSWFPAAVGFLLGGLFLWVANKVIPH
                     LHPNSPMKDAEGINPESRRRSTLLVFAITLHNIPEGLAVGVAFGAVAAGMSSATIAAA
                     IALAVGIGIQNLPEGTAVSMPLRRDGMSRRKSFFYG"
BASE COUNT          551 a          519 c          460 g          640 t
ORIGIN      
        1 atcgatcatt caaattccgt ttcgtcattc accacgtacc agacgtcctt gacgccttca
       61 ccgttcacat caccttgtga aatatctttt gtaaaataat ataggggata ccctttatat
      121 gtcagttgct cctcgccgtt atcttcccgg gtgatctttc cgaaatcggc tttgtcatat
      181 cctgccggaa cttcgtattc accggctttt acaattggcc aattcgcaag gcagtcaccg
      241 gtgcagttac tctttccttc cacatcattt ttgaaataat aaagtgtcat tcctttatca
      301 tccgctaaat agtcgtcaat cttatcatgc gataaaactt gcagagctgg tcctttccaa
      361 ccccatttac gaaagacttc ctgggcttct tccgtttgta aaaacgctaa aaactttaca
      421 gcttgtttgt tttttgatcc tttttcggtc aatgcgatag gcgtacctct atacagcttg
      481 tccttcaatt tgacaagatc ggttacatcc tggagtcggt aatgccacga ctcgtacgtg
      541 atccaagcgt caatcgtcga atctgatttc cacaactcaa tcgcttcggc acttgttttc
      601 acagatgtcg caatgttttt ccggatatcg gggattagac tttcacgtcc agccagatct
      661 tcccatagtc caagctgacc cgcgccgttc acatcgacaa ttcgcacgcc atctttggct
      721 aggtccttca tcgaatgaat gtttttagga ttccctttcc tgacaaggat gcctgctgga
      781 cgggaataaa gctcgaccca cgactcttca tccagcaatt ccgggtggga aaacgagaag
      841 ttctgcagca tgtaggagga cccgccataa atgatgtccc catctttctt tgcctgttca
      901 atccatttgc cttccggtcc tccagtgacg tggacttgaa tgcctttccc tttggagtat
      961 cgcttcgcaa gttcttccat aggcccgagc ggaccaccag gaccatacac atagacggtg
     1021 ttgggattct ttttcgcctc agcattctta attgacaatc cgattgtcgt catcagacca
     1081 gcgaacagta aaatcgcaaa caatagatta ctcattttct tcatttcaaa tcctcctttg
     1141 gaataaatac cttcactaaa ggtaacgagg ataatggtca aacggtttga aattaatttt
     1201 ctggaccggg caactaaaaa agaagaaagg aaaatatccc tttcttcttc actaagaaag
     1261 tacttattct ttcaacctgg caatcgtcat catcttacac tgacctcatt cgttgtcagt
     1321 acacgcgacg cctcccgtac tttccttgca aaagtgagtg ggtgatcgat cgattcccaa
     1381 tgcatgaact ataaactcca tctgaatttt tataccgagc aacttgttta gtatatgcat
     1441 atggatagta ggggggtgat agaagaccaa agagcagccc gtcagcatcg tttgcatttc
     1501 gtttaaaata aatgttgcac aagggaaaca ttcagcatat aatagcacta ttaccatgat
     1561 caaaatgagt aaggagaata aatatgatgg attatttttt aggactgagt ccagtctacc
     1621 aagcgttgat cgccacttta ttcacgtggg ggatgactgc tgtcggggct gccttggtct
     1681 ttacgacgaa gaaagttaac cagaaattga tggatggaat gcttggattt gcaggcgggg
     1741 tcatgattgc ggcaagtttt tggtcgttgc tttcacctgc cattgaaatg gccgaggaaa
     1801 atagttcctt cccgtcctgg tttccagcag ctgtcggctt tttattggga ggtctctttc
     1861 tatgggtagc aaataaggtc atccctcatt tgcatccgaa ctctccgatg aaagacgcgg
     1921 agggcatcaa ccctgagagc agacgacgca gcacgcttct tgtttttgcc ataaccttgc
     1981 acaatattcc tgaagggctt gcagtcggtg tagctttcgg ggcagtggca gccggtatgt
     2041 catcggcaac aattgccgca gcaattgcgt tggctgttgg aatagggatt cagaaccttc
     2101 ccgaagggac agctgtatca atgccattac ggagagacgg aatgtcccgt aggaaaagtt
     2161 tcttctatgg
//
LOCUS       DASULI010000274         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1269128,
            whole genome shotgun sequence.
ACCESSION   DASULI010000274 DASULI010000000
VERSION     DASULI010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269128"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..444)
                     /locus_tag="VFX34_05860"
     CDS             complement(187..444)
                     /locus_tag="VFX34_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HEX5564464.1"
                     /translation="MATLETKLMLALFLPGLLVVFFTRVTFHHFVGLALTIALIAASV
                     YAGYTHNWILYAADALSLTVGFWYATRMVKKAKKQGSQVDE"
     gene            complement(480..1649)
                     /locus_tag="VFX34_05865"
     CDS             complement(480..1649)
                     /locus_tag="VFX34_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX5564465.1"
                     /translation="MSEQVLMDLLQAIALFFLNPVWLVALVAAVGLGYFRVKRERRSF
                     LVRLHPGLTEMKKLLAESWLPALIVSVLISGIGLTVDAGWIVLFSAVAILSMLSFYYQ
                     VASPVYFAAIAFFGLYALQQWGPDFAYRGWTPGDVDLFGDMALTVPVIAGLFLIAEGI
                     LVRRHTAAYASPFLAKTNRGLRAAAYKSKRLWLLPVVFVIPGDLLSAITPYWPQFTIG
                     AGVFSFLPVPIVIGFSQVARASFPDVLFPKIGQAITVLGSVVVVVGAFAWWMPILGWA
                     ALLIGVVCRAVLSILVAVRERKGGFAAAPQSTGVIIAGILPDSPGEKLGLVPGECIRK
                     VNGIQVSNEKELYDAIQVNAAHCRLQVIGRDGEVRLMQQVIYRHDHHRLGLLVVR"
     gene            complement(1990..>3261)
                     /locus_tag="VFX34_05870"
     CDS             complement(1990..>3261)
                     /locus_tag="VFX34_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5564466.1"
                     /translation="YPVEGDRLIEGALRGMADVIGDPYSTYLSEEEAASHRESLAGEK
                     VGIGAEITRSGGRYIIVAPIKGSPAEKAGLQPYDEIVRINGEALGGLTLQDVVRKIRG
                     KEGTALEMTIYRPDLNKHLELKVMREVIPVKTVTAELMEERGTKFGYIALTMFGDETA
                     AEWKKATDAMIKGGAEALVIDVRGNPGGYLRAVGEVAGSLLKDDTVFAYMQNAKGELT
                     PLVVEPSEKFKFDEKLKKMPVVLLQDKGSASASEVLSGALKDLRRGFIIGQVSFGKGT
                     VQDTTELSNGGEMKLSTHKWLTPKETWIHGKGVNVDLASEQNKLFTEHIRFSTDVYAV
                     GDYHEDIAYGQRILSGLGYELERDDGYFDESTAVAVHEFRVNAKVSPESIMDRKFFSA
                     IRERVEDYRKDKANDIQLQVAIGYVHHLLND"
BASE COUNT          793 a          959 c          741 g          768 t
ORIGIN      
        1 atcgcatttc tcggtggtgg cgtcatgcca gtcgtcctcg gtacgatcgg cgtgcttgcc
       61 atctatagtt ttttcatgtg attctttttt ggggaatcgc gtcatagtga atgatggctg
      121 cgccaatccg tagctgtccg caaaaatcta aaaggccgcc ccgttgccgg agcggccttc
      181 ccttattcat tcatcaacct gacttccttg cttcttcgcc ttcttcacca tccgcgttgc
      241 ataccagaaa ccgaccgtca gcgaaagcgc atccgccgca tacagaatcc agttatgcgt
      301 atagcctgca tacaccgatg ccgcgatgag cgcaatcgtc aacgcaagcc cgacaaaatg
      361 gtggaacgtg acccgcgtaa aaaatacgac gagcaaacca ggcaagaaca aggcgagcat
      421 caatttcgtt tccaatgtgg cgatatccat tttccaccaa acctttttgc tgaattcagt
      481 taacggacga caagcagtcc taaccggtga tgatcatgac ggtaaatcac ttgctgcatg
      541 agccgcactt caccatctcg cccgatcact tgcagacggc aatgagcagc attcacttga
      601 atcgcgtcat acaattcctt ttcattcgac acttgaatgc catttacctt acggatgcat
      661 tcccctggaa ccaatccgag tttttcaccc ggcgaatccg gcaagatgcc cgctatgatg
      721 acgcctgttg attgaggcgc agccgcaaat ccgcccttcc gctcccgtac agcgacaagg
      781 atggaaagca ccgccctgca gacgacaccg atcagcagtg cagcccagcc gagtatcggc
      841 atccaccatg caaacgcccc gacaacgacg acaacagacc ccagtacggt gatcgcttgt
      901 ccgattttcg ggaacagcac atccggaaag cttgcccggg cgacttggga aaacccgatg
      961 acaatcggca ccgggaggaa actgaacacg ccagctccta tcgtgaattg cggccaatag
     1021 ggcgtgattg ccgacaggag gtcccctgga atgacaaaca cgaccggcaa tagccaaagc
     1081 cgtttggatt tataggcagc agcgcgcaat ccccggttcg tctttgccaa gaacggagaa
     1141 gcgtaggctg ctgtatgccg ccggacgagg attccttccg caataaggaa tagccccgca
     1201 atgactggaa ccgtcagcgc catatccccg aacaggtcga cgtcacccgg cgtccatccg
     1261 cggtatgcaa agtcgggacc ccattgctgg agcgcataca gaccgaaaaa tgcgatagcc
     1321 gcaaaataga caggtgatgc aacctgatag taaaacgaga gcatcgataa aatggcgact
     1381 gccgaaaata gcacgatcca gccagcatcg acggtcaagc cgattcccga gatcaatacc
     1441 gaaacaatca gcgccggcag ccacgattcg gcgagcagct ttttcatttc cgtcaagcct
     1501 ggatgaagcc gcacgaggaa gctgcgccgc tcccgcttca ccctgaagta ccctaacccg
     1561 accgccgcca ctagggcaac caaccaaact ggattcagga aaaatagtgc aatagcttgt
     1621 aacagatcca tcagtacttg ttcactcatc aaatcgtcac catcccatcc ccgaaaacat
     1681 gattattccc tattgtatca aaagaatcgt ctcacgtgtg agaccgccga taaaatttct
     1741 atgaattgag ccttctattt caatcgtaga ttactatttg ggcaaaaaaa agactccctg
     1801 ctagagcagg gagcctagaa tggtggagag taattgagcc cttgtgcgat taatggagcc
     1861 tttgttggaa taatttagcc cttgcgagat taatggagcc cttgatgaaa taattgagcc
     1921 cttgcgagat taatggagcc cttgatgaat taatggagcc cttgatcatt taattgagcc
     1981 tttccctctt caatcattca acaaatgatg cacatatcca attgcgacct gcaactgaat
     2041 gtcgttcgct ttatctttgc gatagtcctc gacgcgttcg cggatcgcgg agaagaattt
     2101 gcggtccatg atgctttcag gagacacttt tgcattcacc cgaaattcat ggacggcaac
     2161 ggctgtcgac tcgtcgaaat agccgtcatc ccgttcgagc tcatagccga ggcctgatag
     2221 gatccgttgt ccgtaggcga tatcctcatg atagtcgccg accgcataga catccgtcga
     2281 gaaccggata tgctcggtga aaagcttgtt ttgctcagag gccaagtcta cgttgacgcc
     2341 cttcccgtgg atccacgtct ctttcggcgt cagccattta tgggtcgaca atttcatctc
     2401 gccgccgttc gaaagctccg tcgtatcttg gacggtccct tttccgaagc tgacttgacc
     2461 gatgatgaag cctcttcgca aatctttcaa tgcaccgctc aacacttcgc tcgccgatgc
     2521 gctgcccttg tcttgcagga ggacgaccgg catcttcttc agtttttcat caaacttgaa
     2581 tttctctgaa ggttccacaa cgagcggcgt caattcgcct ttcgcatttt gcatgtaagc
     2641 gaagaccgta tcatccttca ataaactgcc ggcaacttcg ccgacggcac gcaaataacc
     2701 gcccggattg ccgcgcacat caatcacgag tgcctcggca ccgcctttga tcattgcatc
     2761 ggtcgccttt ttccattcgg ccgccgtttc atcaccgaac atcgtcaacg ctatgtaacc
     2821 gaatttcgtg cctctttcct ccatcaattc agctgtcacc gttttcacag gaatgacttc
     2881 gcgcatcact ttcaactcaa gatgcttatt caggtccggc ctgtaaatcg tcatttccaa
     2941 cgcggtccct tccttgcccc ggattttcct gacgacatcc tgcaatgtca atccgcctaa
     3001 cgcttccccg ttaatgcgca cgatttcatc ataaggctgc agacccgctt tttcggctgg
     3061 agatccttta atcggagcaa caatgatata cctgccgccc gaccttgtga tttccgcacc
     3121 gattccgacc ttttcccctg cgagcgactc cctgtgcgac gcggcttcct cctccgaaag
     3181 gtacgtggaa tacggatccc cgatgacgtc cgccatgcct ctcagcgccc cttcgatgag
     3241 cctgtcccct tcgactgggt a
//
LOCUS       DASULI010000275         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_22071, whole
            genome shotgun sequence.
ACCESSION   DASULI010000275 DASULI010000000
VERSION     DASULI010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22071"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFX34_05875"
     CDS             complement(<1..742)
                     /locus_tag="VFX34_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEX5564467.1"
                     /translation="MQESVAAKAQTVNEFIHDGNVDMRSGDIRFDGNVRIGNDVETSM
                     FVGATGKVIIQGAVQKATVEAGTSAIIEGNVLSSTVEVGMQEVLVDGLASQLSGLLVY
                     LDRINKATLEIINIRGVRPAEVDASELKDLVRVTLKEEYLDFQHDKFEFIHKAQNHST
                     QLSSEWMTVVEKLHNIFTDTSLTVVRNAEEFSTLLKEAHMLVEQYSAMGCGGSRLKLP
                     YAINSVLSCNGTIEVTDSGLYNCSVTAKK"
     gene            complement(1011..1898)
                     /locus_tag="VFX34_05880"
     CDS             complement(1011..1898)
                     /locus_tag="VFX34_05880"
                     /inference="COORDINATES: protein motif:HMM:NF042666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6612 family protein"
                     /protein_id="HEX5564468.1"
                     /translation="MKKWLKGLGVGVLALGLAACGSAAEPKKDAETGEEVELENKSEM
                     TAQEVYSKAMEASAEQKSMHAVMDIDQKISIPSQELDMKSKIKMDMDIIVEPLAMHQN
                     MKMDMGEMGKMDMELYMNESGFFMNDPESGQWIKMPKDMYDEMMAQMGGETDPTLDMK
                     MFNEFKDDFKFEQTDDEYILTLSASGDKFSGLMKELMGSALPADMDMTEEDAEMLENM
                     DVKSLEVVMYIDKETFYTNAFDLDMDMTMKVEEEEMRIVQKMKSVISKINEIDEIVVP
                     QEVIDEAVDLEELMNQSQE"
BASE COUNT          539 a          577 c          401 g          723 t
ORIGIN      
        1 gcttttttgc agtgacggag caattgtaaa gaccggaatc cgtcacctca atcgtaccgt
       61 tgcacgaaag cacactgttg atggcatacg gaagtttcag ccgcgaacct ccgcatccca
      121 ttgccgaata ttgctcaaca agcatatggg cttccttcag aagcgttgaa aattcctccg
      181 cgttcctgac aactgtcaaa gacgtatccg taaagatgtt gtgcaatttt tccacgaccg
      241 tcatccattc cgatgataat tgggtggagt gattttgcgc cttatgaatg aattcaaact
      301 tgtcatgttg gaagtctaag tattcctctt ttagagtgac ccgaacaagg tcttttaatt
      361 cgcttgcatc gacttctgca ggccggacac cccgtatgtt gatgatttca agggtcgctt
      421 tgttgattct atccaaataa acaagaagtc cgctcaactg cgatgccaac ccgtctacca
      481 gcacttcctg catgccgacc tcaacggtgg atgaaaggac gttcccttca atgatggcgg
      541 atgttcccgc ctcgacagtc gctttttgga cggccccttg aatgataacc tttcctgttg
      601 ctccgacgaa catcgaggtt tccacgtcat ttccaattcg gacatttcca tcaaatcgga
      661 tatccccgct tctcatgtca acatttccgt catggatgaa ttcattgaca gtctgcgcct
      721 ttgcagccac ggactcctgc atactctcac ctctagccac ttttttatta ttataatagc
      781 aaaaaaaggc ctgttcacct aatgagtgaa cagacctttt cgcatcaaag ccccgcttta
      841 acctcttcaa tcatctcttt catggacaga gtttgttaat tgatcattaa ttcctgattt
      901 gttggtatta tgcggtttat acaattttga cgggatttca acgctttttg cgcatctgaa
      961 aaagcggtca tcgacagctg tttcagctat ccatgaccgc ttgcttatgc ttactcttgg
     1021 ctttgattca tcaattcttc aaggtcaacc gcttcgtcga tcacttcttg aggaacgacg
     1081 atctcatcga tttcgttgat tttcgaaatg acggatttca tcttttgaac aatccgcatt
     1141 tcttcctctt cgactttcat tgtcatatcc atgtctaagt cgaatgcatt tgtgtagaat
     1201 gtttccttgt cgatgtacat gacaacttcg aggcttttca catccatatt ttccaacatt
     1261 tcagcatctt cctccgtcat gtccatatcc gcaggaagtg cagaccccat caattctttc
     1321 atcagaccgc tgaacttatc gcctgaagcg gatagtgtca agatgtactc atcgtctgtc
     1381 tgttcgaatt tgaaatcatc cttgaactca ttgaacattt tcatatccaa tgtcggatcc
     1441 gtttcgccgc ccatttgcgc catcatttca tcgtacatat ccttaggcat cttgatccat
     1501 tgtccggact ccggatcatt catgaagaaa cctgattcat tcatgtacag ctccatgtcc
     1561 attttgccca tctcgcccat gtccatcttc atgttttggt gcattgccaa cggctcgacg
     1621 attatgtcca tatccatctt gattttcgac ttcatatcaa gttcttgact tggaatggat
     1681 attttttggt caatatccat gaccgcatgc atgctctttt gttctgctga cgcttccatc
     1741 gctttgctgt atacttcctg cgccgtcatc tcacttttat tttcaagttc aacttcttca
     1801 ccggtttctg catctttttt tggttctgcc gcggatccgc aggcagccaa gccaagtgcg
     1861 agaacaccga caccaagacc ctttaaccat tttttcattc gattcgcttc ccttctttgt
     1921 ttctctttat gccacatcta atcatacggt ataaaaagaa aagggttcca ccattttagg
     1981 aatatactta aatttgaatc attcctacca caaaaatcac ctgagccttt cccaccagcc
     2041 acaactattt cgattactga agtttttatg tcccttcgga atgaaattcg ctataataac
     2101 ggggacagta gaaaggacat caaactatgc tgaaaaaatt cttttcctat tataagccac
     2161 acaaacggct tttcatcatc gacttttcaa gcgcaatatt cgtcgctttg cttgagctgg
     2221 cgttcccgat ggctgtccaa
//
LOCUS       DASULI010000276         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_177465, whole
            genome shotgun sequence.
ACCESSION   DASULI010000276 DASULI010000000
VERSION     DASULI010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177465"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFX34_05885"
     CDS             complement(<1..271)
                     /locus_tag="VFX34_05885"
                     /inference="COORDINATES: protein motif:HMM:NF044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEX5564469.1"
                     /translation="MDYLLNGMKLVIGLITFMVVIRLLGKKHLAEMTPYDIIYLIMFG
                     GILEETLYDNKVSIWMFLFSITVWAVAIFAIEKLVAKFNPLRILLK"
     gene            423..899
                     /locus_tag="VFX34_05890"
     CDS             423..899
                     /locus_tag="VFX34_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX5564470.1"
                     /translation="MVQRHVVGYYDNESEAVAAIEELKRQGYSSNDISIISKDREHVD
                     YIEEQTGTHAADGAATGAATGGLLGGLGGVLAGIGALAIPGIGPIIAAGPIVAGITGA
                     AAGAGVGGLAGALIGMGIPEEEAHRYNEYFNEGKILVLVDGEYRNTRMDYPDRPLV"
     gene            complement(944..1492)
                     /locus_tag="VFX34_05895"
     CDS             complement(944..1492)
                     /locus_tag="VFX34_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX5564471.1"
                     /translation="MIQSTEGKPFKKRNYRPAIIILSIVLIGVIGLLSGRQGVEDFKA
                     FDVTILPLLNAIFNSFTFLFLVAAQIAIKKRNVDVHRKFIYAAFCTTALFLVSYVAYH
                     YLSPSTPYGGEGILAGIYYFVLITHILLAAAIVPLALTSVARAWNREHERHRKIVRWT
                     MPIWLYVSFTGVLVYVMISPYY"
     gene            complement(1629..2378)
                     /locus_tag="VFX34_05900"
     CDS             complement(1629..2378)
                     /locus_tag="VFX34_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX5564472.1"
                     /translation="MPILEVKNIKKSFGPLEVLKQISFEVNKNDCIAVIGPSGSGKST
                     MLRSLVHLEELDGGSVSVNGNYIVKDGVYSKPQQVKQITSNMGMVFQHFNLFPHLTVI
                     ENLELAPKLLKQNDSAQTRQLSMELLEKVGLSDKADVLPSKLSGGQKQRVAIARALMR
                     KPEILLFDEPTSALDPELTGEVLRVMKDLAEEDMTMVVVTHEMEFAKDVANKAIFMDK
                     GEIVETGSPVELLSNPQMERTKAFLYRTLSK"
     gene            complement(2411..>2947)
                     /locus_tag="VFX34_05905"
     CDS             complement(2411..>2947)
                     /locus_tag="VFX34_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5564473.1"
                     /translation="AKSSIKPLAYLANAYIYLMRGTPLLLQLLFIVFGLPLIPVIGEY
                     LVLDRFVAACLGFILNYAAYFAEIFRGGLLAIDKGQYEAAQVLGLNRWQTMRKVVLPQ
                     MFRIALPAVANESVTLVKDTALLYAVAVPELLHFAQTAVNRDFTITPFLLAGVIYLLI
                     TLCLTMFFKWLERRLRFD"
BASE COUNT          856 a          662 c          622 g          807 t
ORIGIN      
        1 cttttaggag aatacgcaac ggattgaatt tagccaccag cttttcgatt gcgaatatgg
       61 ctaccgccca tactgttatc gaaaaaagga acatccatat ggaaacttta ttgtcgtaca
      121 gggtttcttc aaggattccg ccaaacatga ttaaataaat gatgtcatac ggagtcatct
      181 ctgccaaatg cttcttcccc aacagccgaa tgacgaccat gaaagttatc aacccgatga
      241 cgagcttcat cccatttagt agataatcca tttctatccc tcctattcat cagttagtta
      301 cccataatga aacttttcaa atctatgggg tataaaattg catgtttcaa aatgaatcgt
      361 tagggtattt tctagaatag caataaacta gaacaaattt tattaattgg aggcgtattt
      421 gaatggttca acgacacgta gtaggttatt atgacaatga atcggaagct gtagcagcaa
      481 ttgaagagtt gaaacgtcaa gggtacagtt caaatgatat ttccatcatc agtaaggata
      541 gagaacatgt cgattacatc gaagaacaaa caggaacaca cgcagccgat ggtgcagcta
      601 ctggtgcagc tactggcggc ctgcttggcg gacttggagg cgtgttggca ggaatcggcg
      661 cgcttgcgat tccaggcatc ggcccaatca tcgccgctgg tccaattgtg gcagggatta
      721 ccggggccgc agccggggcc ggagtcggtg ggcttgcagg ggcgttgatc gggatgggta
      781 ttccggaaga ggaagctcat cgctataatg aatatttcaa cgaaggcaag attcttgttt
      841 tagtagatgg cgaatatcgg aatacacgga tggactatcc tgatcgcccg cttgtgtaaa
      901 gttgaaaagg ctgctcccta tgaatgggag cagccttttc agttcaataa tagggtgaaa
      961 tcattacata gacaaggaca cccgtaaagc tgacatataa ccagatcggc attgtccatc
     1021 gcactatttt tcgatgacgc tcatgttccc tattccaagc cctcgcaaca cttgtcaatg
     1081 caagcggaac gatcgccgcg gcgagtagga tatgggtgat caagacaaaa taatagatcc
     1141 ctgccaaaat cccttcccct ccatatggag tggatggcga caaatagtga taagctacgt
     1201 acgagacgag gaataaagct gttgtgcaaa atgcggcgta tatgaatttc cgatggacat
     1261 ccacattcct tttcttaatg gcaatttggg cggcgactag gaataagaat gtgaagctat
     1321 tgaaaatggc attcagcaaa ggcagaatcg taacatcgaa tgccttgaaa tcttccactc
     1381 cttgcctgcc cgacaacagg ccgatcactc ctattaaaac aattgataaa atgatgatgg
     1441 ctggccgata attccttttt ttaaatggct ttccttccgt cgattgaatc atttaaacat
     1501 ctccttagac tctacattcc ataaagaagt gtaacatatt ttaaacttca ttaacccgac
     1561 ttcacattgt tgtcaaaatg aaaaagcagc cgggacaact ttccgactgc gttgtgaaga
     1621 acggttattc atttggataa tgtacgatat aaaaatgctt ttgtcctttc catttgtgga
     1681 ttgctcaata gttccactgg acttccggtt tcgacgattt cccctttatc catgaaaatc
     1741 gctttgtttg caacgtcttt tgcaaattcc atttcatgtg tgacaacgac cattgtcata
     1801 tcctcttcag caagatcctt catgacacgt agtacttccc cggtcaactc agggtcaagg
     1861 gcagatgtcg gttcatcgaa aagaaggatt tcaggctttc tcattaaagc tctggcaatc
     1921 gctacccttt gtttttgacc accggaaagt tttgacggca atacatcagc cttatcggaa
     1981 aggcctactt tctccaataa ttccatgctc aattgtcggg tttgcgcaga atcattctgc
     2041 tttaaaagct ttggagcaag ttcgagattt tcaatgacag tcagatgagg aaacagattg
     2101 aaatgctgaa aaaccattcc catattagag gtgatctgtt tcacttgttg gggtttagag
     2161 tagactccat ccttcacgat atagtttccg ttgaccgaaa cacttccccc atccaattcc
     2221 tctagatgga ccaaacttct gagcatcgtg ctcttccctg acccggaagg tccaatgacc
     2281 gctatacaat cgtttttatt cacttcaaaa ctgatctgct tgagcacttc aagcggaccg
     2341 aatgattttt ttatattttt cacttcaaga atcggcattt cctttcactc ctcaatttcc
     2401 aacactgaat ttaatcaaac cgtaaacgcc tttccagcca tttgaagaac attgtcaaac
     2461 ataacgtaat gagtagatag atgacgcctg ccagtaagaa tggcgttatt gtaaaatcac
     2521 gatttactgc tgtttgtgcg aaatgcaata gttcgggtac tgccaccgca taaagcaaag
     2581 cagtatcttt caccaaagtg accgactcat ttgcaacggc tggcaaagcg attcggaaca
     2641 tctgcggcaa caccactttt cgcatcgtct gccatctgtt caatccaagc acttgcgcgg
     2701 cttcgtattg ccctttatcg atcgcaagca ggccgccacg gaaaatttcc gcaaaataag
     2761 cggcatagtt caatatgaat cctaagcaag cagcgacaaa tcggtccagg actaaatact
     2821 ctccgataac gggaatgagc ggcaaaccga aaacgatgaa aaggagctgc aataagagcg
     2881 gtgttccccg catcagataa atataggcat tcgccaaata agctaacggt ttgatggaac
     2941 ttttagc
//
LOCUS       DASULI010000277         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_839650, whole
            genome shotgun sequence.
ACCESSION   DASULI010000277 DASULI010000000
VERSION     DASULI010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839650"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFX34_05910"
     CDS             <1..220
                     /locus_tag="VFX34_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5564474.1"
                     /translation="KSTLSRKYAAETGESFSAALLKTRIEEAKRLLKETDLPIAEVSV
                     SAGFTHSTYFSRRFKEETGFTPYQFRMR"
     gene            complement(279..830)
                     /locus_tag="VFX34_05915"
     CDS             complement(279..830)
                     /locus_tag="VFX34_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX5564475.1"
                     /translation="MQNIQRSTHSATRAKTFDLVLTAVLSTLVLVSTVFINIKLPIGQ
                     GGLIHLGTAALFIVSILFGPKKGALAGAIGMGLFDIFGGWAIWAPTTIVARALQGYIV
                     GRIAWSKGHQGDHIGLNILGAIASIPVMLAVYFVGQGIMFGNWIQPFASIPGDLIQNF
                     VGLLIAIPLCVALKKTPFFRKKF"
     gene            1022..1867
                     /locus_tag="VFX34_05920"
     CDS             1022..1867
                     /locus_tag="VFX34_05920"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEX5564476.1"
                     /translation="MKKVAVIQDMSSFGKCSLTAAIPVLSVMGVQAVPLPTAILTSQT
                     EYPSYYCEDLTSKMNHFTDEWSKLGAAFDGIHTGFVTGKEQIENIFSFLHTFYKEGTT
                     LLVDPVMGDRGEMYEALSDELIGYMKELAKHADIITPNITECCLLTGLPYEKLHRYRN
                     NEDYLSAIEEAGRKLHSMTDANVIITGLNPPAISETRFVGNMYIDENRSFHSLQEYNG
                     MSYSGTGDLFASVIMGGMMRDQDVAETMKLAEKFLSAAIETTAKEQIPSVAGVNFESF
                     LRMLL"
     gene            2054..3727
                     /gene="hutU"
                     /locus_tag="VFX34_05925"
     CDS             2054..3727
                     /gene="hutU"
                     /locus_tag="VFX34_05925"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX5564477.1"
                     /translation="MKADTVEKVVQYRGTELNTKGWQQEAALRMLMNNLHPDVAEHPE
                     KLVVYGGIGKAARNWESFDAIVRSLKELENDETLLIQSGKPVAIFKSHTDAPRVLIAN
                     SNIVPAYANWDTFHELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFAELAKQHFGE
                     SLKGTITLTAGLGGMGGAQPLAVTMAGGVCIGIEVDETRIDRRIETRYTDVKTDSLDE
                     AIRLAEEAKAEGKALSIGLLGNAADLLPEMIARGFNPDVLTDQTSAHDPLNGYIPSGM
                     SLEEAAELRAANPDEYVKRSKASMAKHVAAMIEMMDKGAITFDYGNNIRQVAKDEGVE
                     RAFDFPGFVPAYIRPQFCEGKGPFRWVALSGDPEDIRKTDEVILREFSYNTHLCNWIR
                     MAQEKIQFQGLPSRICWLGYGERARFGKIINDMVASGELSAPIVIGRDHLDSGSVASP
                     NRETEAMKDGSDVISDWPILNAMINAVGGATWVSVHHGGGVGMGYSQHAGMVIVADGT
                     KEAEARLERVLTSDPGMGIARHVDAGYDLDIQTAKEKGVNIPMMEGGNS"
     gene            3724..>3922
                     /locus_tag="VFX34_05930"
     CDS             3724..>3922
                     /locus_tag="VFX34_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX5564478.1"
                     /translation="MKPIWIKHAAQLATIAGENKPRKRKEMSELGIIEDGSVWIEDGV
                     IAAIGTTEELEHQFGDRASGAD"
BASE COUNT         1058 a          841 c         1105 g          918 t
ORIGIN      
        1 taaaagtaca ttgagcagga aatatgcagc ggaaacgggc gaatctttca gcgcggcatt
       61 gctgaaaacc cgaattgaag aagcgaagcg tctgttgaaa gagactgatt tgccgattgc
      121 tgaagtttcc gtgtccgccg gctttaccca ttccacgtat ttcagccggc gattcaaaga
      181 ggagacaggc tttacgccgt atcagttcag gatgagatag agattgaaca aaaaagcagc
      241 gaagaaagga aactgccctt cttcactgct ttgccatctt aaaatttctt ccggaaaaaa
      301 ggcgtcttct tcaatgccac gcatagcggg atggcgatca gcagaccgac gaagttctga
      361 atgaggtcac ccgggatgga tgcgaacggt tggatccagt tgccgaacat gatgccttgg
      421 ccaacgaagt agactgccag catgaccgga atggatgcga tggcacctaa gatgttgagc
      481 ccgatatgat ctccttggtg gccttttgac caagcgatcc taccgacgat atagccttgc
      541 agagcgcggg cgacaattgt cgtaggcgcc caaattgccc agccgccgaa aatatcgaac
      601 aagcccattc caatggcgcc ggcaagtgcc ccttttttcg gtccgaatag gatcgatacg
      661 atgaataggg cagcggtacc taaatggatc aagccccctt ggccaatcgg gagtttgatg
      721 ttgatgaata cagttgacac taggacaagt gtagatagta cggcagtaag caccaaatcg
      781 aatgtttttg cccttgttgc agaatgagtg cttcgttgta tattttgcat aggtgttttc
      841 ccttcctcgc ttcatagttt tttgattaac tcaatattac taaagagctg accaccacaa
      901 aagtgtcaga tcggtaaaaa tggtagaggt cagattaaat tgtggtcaat gtattcttgc
      961 ctctttctta atgtggtacg atgtagaaaa gcaagtaccg acgagttagg tgggaatttg
     1021 aatgaagaaa gttgcggtca tccaagatat gtcgtccttc ggaaaatgct cgctcacggc
     1081 ggcgattccg gtcttgtccg tcatgggcgt acaggcagtg ccgcttccga ctgccatttt
     1141 gacgtcgcaa accgagtacc cgagctatta ttgtgaggat ctgacgtcca agatgaatca
     1201 ttttacggac gagtggagca agttgggcgc tgcttttgac ggcattcata cgggtttcgt
     1261 gacaggaaaa gagcagattg aaaatatctt ttcgttttta catacgtttt acaaggaagg
     1321 gaccacgctg cttgtcgatc cggtcatggg cgatcgggga gagatgtacg aagctttgtc
     1381 ggatgaactg attggctata tgaaggaact ggcgaagcat gcggatatca ttacacctaa
     1441 cataacagag tgctgtctgc tgacgggact gccgtatgaa aagctgcatc gctatcggaa
     1501 taacgaagat tatttatctg cgatcgaaga ggcgggacgc aaactgcatt caatgaccga
     1561 cgcgaacgtc atcattaccg gcttgaaccc gccggcaatt tcggaaacaa gattcgtcgg
     1621 caatatgtat atcgatgaga atcgctcgtt tcatagtctc caggagtata atggcatgag
     1681 ctattccgga acaggtgatt tatttgcatc ggtcatcatg ggcggaatga tgcgcgatca
     1741 agatgttgca gagacaatga agcttgcgga gaaattctta tctgccgcaa tcgaaacgac
     1801 ggcgaaggaa cagatcccaa gcgtggcagg cgtgaatttc gaatcgtttt taaggatgtt
     1861 gctataacaa taaactaagc gggcaaattc ttgattgaat caagagaatg cccgtttttc
     1921 attaacaatc atcaaaatag ttcataaata aatacttcgg ttgtcaataa agtataattt
     1981 gacaatcaat tcataaacaa ttatctgaac cttccctata gaatgatagt aacgaaaggg
     2041 aggtcatcag aaaatgaaag cggatacagt ggagaaagta gttcaatata gaggcacaga
     2101 attgaatacg aaaggttggc agcaggaggc ggcacttcgg atgctcatga acaatttgca
     2161 tcctgatgtg gcggagcatc ctgagaagct tgtcgtctat gggggaatcg ggaaggcggc
     2221 gcgcaactgg gagagctttg atgccatcgt ccgttcgttg aaggaacttg agaatgatga
     2281 aacattattg atccaatcgg gtaaaccggt tgcgattttc aaatcacata cagatgcgcc
     2341 tcgagtattg attgccaact cgaacatcgt tccggcatac gccaattggg atacattcca
     2401 tgagctcgac aagaagggct tgatgatgta cggacaaatg acggcgggca gctggatcta
     2461 tatcggttcg caagggatcg tgcaagggac gtatgaaacg ttcgcggagc ttgcaaagca
     2521 gcatttcgga gagtccttga aagggacgat cacgttgacg gctggtcttg gcggcatggg
     2581 tggcgcgcag ccattggcgg tgacgatggc aggcggcgtc tgcatcggca tcgaagtcga
     2641 tgaaacacgg attgaccgtc gtattgaaac gcgttataca gatgtgaaga cagattctct
     2701 tgatgaagcg attcgtcttg cggaagaagc gaaggcggaa gggaaagcgc tgtcgattgg
     2761 acttctcggg aacgcggcgg atctattgcc agaaatgatt gcacgcggat tcaatccgga
     2821 cgttttgacg gaccagacat ctgcgcatga cccgttgaac ggctatatcc cgtcgggcat
     2881 gtcgcttgaa gaggcggcgg agctccgtgc tgcaaatccg gatgagtacg tgaagcgttc
     2941 gaaagcatcg atggcgaagc atgttgctgc gatgatcgag atgatggata aaggtgcaat
     3001 cacattcgac tacgggaaca acatccgtca agtggcgaag gatgaaggcg ttgaacgggc
     3061 attcgatttc cctggattcg ttccggcgta tatccgaccg cagttctgcg aagggaaagg
     3121 acctttccgc tgggtggcgc tttccggaga cccggaggat atccggaaaa cggacgaagt
     3181 catcttgcgt gaattcagct ataatacgca tctttgcaat tggatccgga tggcacagga
     3241 gaaaatccaa ttccaagggc ttccttctcg tatttgctgg ttagggtatg gcgagcgcgc
     3301 tcgtttcggg aagatcatca acgacatggt tgcgagcggc gaattaagtg cgccgattgt
     3361 catcggacgc gatcacctcg attcaggttc agtcgcttcc ccgaaccgcg agacggaagc
     3421 aatgaaggac ggatcagatg tcatttcgga ctggccgatc ctgaacgcga tgatcaatgc
     3481 ggtcggcggg gcgacttggg tatccgtcca ccatggcggc ggcgtcggca tgggctattc
     3541 ccagcatgcg gggatggtca ttgtcgcgga tggaacgaag gaagcggaag cgcgtcttga
     3601 acgtgtgttg acatcggatc cgggaatggg catcgcacgc cacgttgacg cgggctatga
     3661 tctcgatatt caaacggcga aggaaaaagg ggttaacatc ccaatgatgg aaggtggaaa
     3721 ttcttgaaac caatctggat caaacatgca gcacaactag ctacaatcgc gggtgagaac
     3781 aagccgcgta agcgcaagga aatgtctgaa ctaggcatca ttgaagacgg cagtgtgtgg
     3841 attgaggatg gtgtcatcgc agccatcggg acgaccgagg agttagagca tcaattcggc
     3901 gaccgggctt ccggtgcgga ca
//
LOCUS       DASULI010000278         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_293088, whole
            genome shotgun sequence.
ACCESSION   DASULI010000278 DASULI010000000
VERSION     DASULI010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293088"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFX34_05935"
     CDS             complement(<1..441)
                     /locus_tag="VFX34_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX5564479.1"
                     /translation="MKFFIDTANFEEIKEAHSWGILSGVTTNPSLVAKENISFHDRLR
                     EITALVPGSVSAEVIALDAEGMIEEGRELAKIAPNITVKLPMTPEGLKACSVFAQEGI
                     KTNVTLIFSANQALLAARAGATYVSPFLGRLDDIGQEGIHLISTI"
     gene            complement(533..1390)
                     /locus_tag="VFX34_05940"
     CDS             complement(533..1390)
                     /locus_tag="VFX34_05940"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003346852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX5564480.1"
                     /translation="MALVSMKEMMIKGKNEGYAIGQFNLNNLEYTQAILQAAQEENSP
                     VILGVSEGAGRYMGGFTTVVNMVKGLMHDYNITVPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASSKPLAENIEITKKVVEYAHAKGVSVEAELGVVGGQEDDVIADGVIYADPAE
                     CKELVDSTDIDCLAPALGSVHGPYKGEPNLGFKEMEEISSQSDLPLVLHGGTGIPTKD
                     IQRAISLGTAKINVNTENQIEGTKAVRETLNADANVYDPRKYLAPMRDAIKKTVIGKM
                     REFGSSGKA"
     gene            complement(1527..1892)
                     /locus_tag="VFX34_05945"
     CDS             complement(1527..1892)
                     /locus_tag="VFX34_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5564481.1"
                     /translation="MKSLLIVDDQAGIRLLLNEVFTKEGFEARLAANGTEALQSVKEK
                     EPDCVLLDMKMPGIDGVEVLKRLKKDWPHIPVIMMTAYGEVELTEEAIENGAEKYFTK
                     PFNIYEVRDAVKEILLHRK"
     gene            2067..>2222
                     /locus_tag="VFX34_05950"
     CDS             2067..>2222
                     /locus_tag="VFX34_05950"
                     /inference="COORDINATES: protein motif:HMM:NF022200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 family protein"
                     /protein_id="HEX5564482.1"
                     /translation="MKILTTQIGGLLQRAGSNNEEAVEETARLLAQATIGEGRVLFAG
                     FDEMEAVG"
BASE COUNT          493 a          504 c          517 g          708 t
ORIGIN      
        1 gattgtgctg atcagatgga ttccttcttg accgatatca tcgagtctgc caaggaatgg
       61 ggatacataa gtggctccag cgcgcgctgc aagcaatgct tgattggcac tgaaaatgag
      121 ggtcacgttt gttttgatgc cttcttgtgc gaaaacggag catgctttca agccttcggg
      181 tgtcattgga agtttgacag tgatgttcgg agcgattttc gctagttcgc gtccttcttc
      241 aatcatgcct tccgcgtcga gtgcgatgac ttcagcgctg acagatccag gtacgagagc
      301 tgtgatttcg cgcagtctgt catggaatga gatgttttct tttgcaacga gtgatgggtt
      361 tgttgtaacg ccggatagga ttccccagct atgtgcttct tttatttcct caaagtttgc
      421 cgtatcgatg aaaaatttca tgtgaaaagt tcctccttca atgaatggaa aagaaggaga
      481 atgcatccgt tttcgatgct tctcctattt atatgtccaa atttattaca agttaagctt
      541 tacctgaaga tccaaactca cgcattttgc cgatgactgt tttcttgatg gcgtctcgca
      601 ttggtgccag gtatttacgt ggatcataga cgtttgcgtc cgcgttcaat gtttcacgga
      661 ctgcttttgt gccttcgatc tggttttcgg tgttgacgtt gatcttcgca gttccaagtg
      721 agatggcgcg ttggatatct ttcgtcggga tgcctgttcc accatgaagg acaagcggaa
      781 ggtccgattg gcttgagatt tcttccatct ctttgaatcc taggttcggt tcgcctttgt
      841 acggaccgtg gaccgatccg agtgccggtg ccaggcagtc gatgtccgtg ctgtcgacta
      901 gctctttaca ttccgccgga tctgcataga tgacgccgtc agcgatgaca tcgtcttcct
      961 gtcctccgac gacaccgagc tccgcttcga cggatacgcc ttttgcatgt gcatattcta
     1021 caactttttt cgtgatttca atgttttctg caagaggttt ggaagatgcg tcgatcatga
     1081 cagatgtgaa gcccgcgtcg atcgcctctt tgcatttttc gaaactggat ccgtggtcca
     1141 gatggatagc gacagggacg gtgatgttgt aatcatgcat caaccctttc accatgttga
     1201 ctactgtcgt gaatccgccc atatagcggc ctgccccttc ggaaacaccg aggatgaccg
     1261 gggaattctc ttcttgcgcc gcttgcagga tcgcctgcgt gtattcaagg ttgttcaaat
     1321 tgaactggcc gattgcatat ccttcatttt tgcctttgat catcatttct ttcattgaaa
     1381 caagtgccat tgcaaaatcc tcctccggat cgttgagatg aatttatgtt tgaatcgata
     1441 tttacctctt ttatcataac aaaaacagac agtatttact actgtctggg ttgtgggcac
     1501 agtgcgtcat cgttttctat ttttatttat ttccgatgaa gcaatatttc tttgaccgcg
     1561 tcgcgcactt cataaatatt gaatggtttc gtaaaatatt tctctgcccc gttttcaatt
     1621 gcctcttctg tcagttcaac ttccccgtaa gcggtcatca tgatgaccgg tatatgaggc
     1681 caatcttttt tcagcctttt caacacttcc actccatcga tccccggcat tttcatgtcg
     1741 agtaaaacgc aatcaggttc tttctcttta accgattgta gagcttctgt gccatttgcg
     1801 gccaatctcg cttcaaaccc ttctttcgtg aatacctcat tcaataaaag tcgtattccg
     1861 gcttgatcgt cgacgattag caaactcttc aaaaggctca cccctttttt attaattgcc
     1921 ccaataagtt atacaatttg acaaaaaacc tgaaaaccct tcttttttta atcattattc
     1981 caatattgtt ttattattcc tggaacgatg ctggattgct tggttttatt gccgggaata
     2041 aatataattg ggacgaggtg gttgagatga agatattgac gacacaaatt ggcgggctgt
     2101 tgcagcgtgc cggttcgaat aatgaggagg ctgtcgagga gacggcgcgg ttgttggcgc
     2161 aggcgacaat cggggaagga cgtgtacttt tcgccggctt cgatgagatg gaagccgtcg
     2221 ga
//
LOCUS       DASULI010000279         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_80506, whole
            genome shotgun sequence.
ACCESSION   DASULI010000279 DASULI010000000
VERSION     DASULI010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80506"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /gene="pckA"
                     /locus_tag="VFX34_05955"
     CDS             <1..1118
                     /gene="pckA"
                     /locus_tag="VFX34_05955"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX5564483.1"
                     /translation="APTFKADPAVDGTKSETFIIVSLEKRIVLIGGTEYAGEMKKSIF
                     SVMNYLLPERGILSMHCSANVGDEGDVALFFGLSGTGKTTLSADANRKLIGDDEHGWS
                     DHGVFNIEGGCYAKTINLSAEKEPEIYGAIRFGSVLENVVLDEKTRKPDYDDNTLTEN
                     TRAAYPIHYIDNIAVPSAAGHPKTIIFLTADAFGVLPPISKLTKEQAMYHFLSGFTSK
                     LAGTERGVTSPEATFSTCFGAPFLPLPATVYAEMLGKKIDEHNAQVFLVNTGWTGGEY
                     GVGKRMALKHTRTMVRAAIAGELNDIQFEQNAIFGLAMPETIDGVPSEVLNPRNAWSD
                     PAAYDQKAKELADLFRENFKKFGHVSEEITQKGGPIA"
     gene            complement(1474..>2242)
                     /locus_tag="VFX34_05960"
     CDS             complement(1474..>2242)
                     /locus_tag="VFX34_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5564484.1"
                     /translation="RRDYPSPNPNIRLEEITYWSDGLKVKGLLAEPVSGGNYEGIIYL
                     RGGIQHVGMVRPARIAQFASQGFVVFAPYYRGNRGGEGRDEFAGDDRQDAVNAVEVLK
                     QSPKLNKDRIHLYAFSRGGIMALWTAILREDITSVVTWAGVSDVVFTYNEREDMRRMM
                     KRVIGGTPNSMPEAYRERTALFRVDEIDAPVLIIHGMKDDNVSFEQAILLEDALREKE
                     KQYETWYFPEYTHYFPTAHNAQIVRDLCDWMRQQGNE"
BASE COUNT          616 a          586 c          493 g          547 t
ORIGIN      
        1 cagctccaac gttcaaagcg gatcctgcag tggacggaac gaaatccgaa accttcatca
       61 ttgtatcact tgagaagcgc attgtgttga ttggcggtac ggaatatgca ggagaaatga
      121 aaaaatcgat tttctctgtc atgaactact tgctccctga gcgcggaatc ctttccatgc
      181 actgctcggc aaacgtaggt gatgaaggtg atgtcgcttt attcttcgga ctttccggta
      241 ctggaaaaac gacactttcc gcagatgcca accgtaaatt gatcggtgac gatgagcacg
      301 gctggtccga tcatggcgta ttcaatatcg aaggcggctg ctacgcaaaa acgatcaatc
      361 tttcagcaga aaaagaaccg gagatttacg gagcaatccg tttcggttcg gtccttgaaa
      421 acgtcgtctt ggatgagaag acgcgcaagc cggactacga tgacaataca ttgactgaaa
      481 atacgcgtgc agcgtacccg atccattata tcgacaatat tgcggttccg tctgcagcag
      541 gacatccaaa gacgatcatc ttcttgactg cagatgcatt cggcgtattg cctccgatct
      601 cgaagttgac gaaagaacaa gcgatgtacc acttcctaag cggcttcact tcgaagcttg
      661 caggaactga gcgcggtgtt acttcaccgg aagcaacatt ctctacgtgc ttcggtgccc
      721 ctttccttcc attgcctgca acggtgtacg cagaaatgct cgggaagaaa atcgatgagc
      781 ataatgcaca agtattcctt gtcaacacgg gatggaccgg cggcgaatac ggggtaggta
      841 aacgtatggc attgaaacat acacgtacaa tggtccgtgc agcaattgcc ggcgaattga
      901 acgatattca gttcgaacag aacgccattt tcggtcttgc tatgcctgaa acgattgacg
      961 gcgtaccgtc cgaagtgttg aaccctcgta atgcatggag cgatccagct gcatatgatc
     1021 aaaaagcgaa ggaattggca gatcttttcc gtgagaattt caagaaattc ggtcatgttt
     1081 ctgaagaaat cactcaaaaa ggcggaccaa tcgcataaac aaaagcggaa gacggcgtta
     1141 agccaatcaa acagcgaagg gtttgatttg ccttaatttc tgcagatctt tgcagaaatt
     1201 aaggcatcca accgtgctcg taacgctacg ctttactcgc aaaagccgtt cttcgtaacg
     1261 gctttggctg ccgtctgtag ctagatgata aaaaaaatcc aggtgcactt ttgcacctgg
     1321 atttttttta tgaaaaccgc ctaataacct tgccgaacca cctatataca agccggaaca
     1381 gcctattaaa ccagcaagac cggctaatcc cgcaccagaa ccgcctaatg acccggcaag
     1441 accgcctata ctcgtatcgc accccgcaac caattattca ttcccctgct gtctcatcca
     1501 atcacataaa tcccttacaa tctgtgcatt atgagcagtt gggaagtaat gggtgtactc
     1561 agggaaatac catgtttcgt attgcttttc cttttcccgt aaagcatcct ccagcaaaat
     1621 tgcctgctca aaagaaacat tatcatcttt catgccatgg ataatcaata caggagcatc
     1681 gatttcatca actctaaata acgctgtgcg ttcccgatat gcctcgggca tgctatttgg
     1741 cgtgccgccg atgacccgtt tcatcatccg ccgcatatct tcccgctcgt tatatgtaaa
     1801 gacgacatcc gagacacctg cccatgttac tacagacgtg atatcctcac gtaaaatcgc
     1861 tgtccacagc gccatgatgc cgccacggga gaaagcatac aggtgaatac ggtccttgtt
     1921 caacttggga ctttgcttca gcacctcaac agcattgact gcatcctggc gatcatcccc
     1981 tgcgaattca tcgcgtcctt caccgcccct attcccacgg taatagggag caaaaacgac
     2041 gaaaccttgt gaagcaaact gggcaatacg cgctggacgg accatcccga catgctgaat
     2101 gcctccccgc aaatagatga ttccttcata attccctccg ctaaccggtt cggcaagcaa
     2161 tcccttcact ttcaatccat ccgaccaata cgttatttcc tcaagacgaa tgttgggatt
     2221 cggcgatgga taatccctcc gg
//
LOCUS       DASULI010000280         5488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_725002, whole
            genome shotgun sequence.
ACCESSION   DASULI010000280 DASULI010000000
VERSION     DASULI010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725002"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..619)
                     /gene="thiT"
                     /locus_tag="VFX34_05965"
     CDS             complement(62..619)
                     /gene="thiT"
                     /locus_tag="VFX34_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEX5564485.1"
                     /translation="MDRKRLQLMLEVAILGALSFVLDNLTLFKMPQGGSVTLSMLPII
                     LMAYRWGVAGGLLTGLISGLLQAVIGGYIIHPVQGALDYFVAYTVVGLAASTHSWLIK
                     SKLAGRKGSMAAAIIAGTVLGGLLRYLAHFIGGMVFFAEYAGDQPVWLYSLVYNGSFM
                     IPSIILCAIVAVILFTSAPRLIQKA"
     regulatory      complement(809..910)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1003..1344
                     /locus_tag="VFX34_05970"
     CDS             1003..1344
                     /locus_tag="VFX34_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX5564486.1"
                     /translation="MTNHKLLSALCYFSIFFSPLLLPAIVYFVTDDHEVKGHAKRSLI
                     SHLVPVVLLIAGFILFSFSMISYESMMHTMMTGRFDFWGLAPFLFMIIYGLLFLFVVI
                     WNVYQGVKVLK"
     gene            1435..2181
                     /gene="map"
                     /locus_tag="VFX34_05975"
     CDS             1435..2181
                     /gene="map"
                     /locus_tag="VFX34_05975"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5564487.1"
                     /translation="MIVKNNEEIEALKKIGRIVAEVREAMKAATVPGITTKEIDELGG
                     RLLAERGAVSAPIDQYEFPGYTCISVNHEVAHGIPGSRVIQDGDLVNIDVSASFGGYF
                     ADTGISFVVGTADEKKQLLCDVAKNAFDRAMTKVKAGSSLNQIGKAVEREARDNGLKV
                     IKNLTGHGIGTSLHDEPQHILNYYDAWDKTLMKEGMVLAVEPFISEKAEHIIELGDGW
                     TFVTPDKSLVAQIEHTIIVTKDEPIILTQL"
     gene            complement(2569..3729)
                     /locus_tag="VFX34_05980"
     CDS             complement(2569..3729)
                     /locus_tag="VFX34_05980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5564488.1"
                     /translation="MSFLEKMDDNIKNKWKFERETPIQSQMIPELLNGRDVVAESPTG
                     TGKTLAYVLPILQQVDGKKMQTQALIMAPSQELAMQIVNVIREWVEGTDITVTQLIGG
                     ANMQRQIERLKKKPTIVVGTPGRLVELVKAKRLKMHDIHYIVLDEGDQLLSRDYRVIV
                     KDLIEAANPERQVAVVSATITDEIELVAKKLMNDPIRLHVSVEDMPLSGKVVHSFVKT
                     DARDKTDLLRGLSHLPSIRALAFMNNVDQLRMKEMKLQYNEAPVAVLYSSMTKFERQE
                     TLENFRKGEIRILIATDLAARGLDIQGLTHVIHVDVPHTIEQYLHRSGRTGRAGEDGE
                     VLTLLSYPEERSYRKLTKGFKPVQKVWYKGKLTEGNSKTVSGQQDVKKTKKK"
     gene            complement(3791..5239)
                     /locus_tag="VFX34_05985"
     CDS             complement(3791..5239)
                     /locus_tag="VFX34_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5564489.1"
                     /translation="MNRKLDEMQFEWLEEATIRQMQERMSTGRLTSEELTVMYMKIIS
                     ERDRNINAVLELNPQALQIARSLDAERRNGIVRSLLHGIPVLLKDNMDTGDGMHTSCG
                     SLAMKNYYAKEDAFLVKKLRDAGAVILGKTNMTEWANFMSDRMRNGWSSRGGQVNNPY
                     GLFDVGGSSSGAAAAIAANMAAVSIGTETSGSIINPSAQNSLVGIKPTVGAISRSGII
                     PLSFTQDTPGPLARTVEDAAITFGLMVGHDETDPVTREARRFEGVDWLSMLDASSLEG
                     KRIGIARSIFVREISTERKEKFEEALRRLDSLGAELVDNIDLGTMEDDLGYDVLLYEF
                     KAALNEYLGKTPMTNPIRTLSDIIQFNKEHPEETLDFGQVMLEKVDRTSGTLTEPEYI
                     EALLRNRHLAGELALDRAFDEHKIDVLVFPQDHGCSFSAAAGYPAITVPSSFTEEGEP
                     FCITLCGKAFSEPELVGYAYAFEQSAKARRKP"
     gene            complement(5259..5411)
                     /locus_tag="VFX34_05990"
     CDS             complement(5259..5411)
                     /locus_tag="VFX34_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fur-regulated basic protein FbpA"
                     /protein_id="HEX5564490.1"
                     /translation="MKKQENPVLENKRDKIIENLVNEGVFKINGKQLYELSLYELMKQ
                     YTMRIE"
BASE COUNT         1378 a         1399 c         1204 g         1507 t
ORIGIN      
        1 aagcgcatgg acaacaaaaa tagagtaggc gcatgaattc atcatatgcc tactcctttt
       61 tttatgcttt ttgaataagc ctaggtgcag atgtgaataa aatcactgct acgatagcgc
      121 acaatatgat ggatggaatc ataaaggaac cattgtatac caaggaataa agccatacgg
      181 gctgatcacc ggcatattca gcaaaaaaca ccatgccgcc aatgaagtgc gccaagtatc
      241 ggaggagccc tcccagtact gttccagcaa ttatcgctgc ggccatcgag cctttacgtc
      301 ccgccaattt gctctttatc aaccaactat gcgttgaagc ggccaaacca actacggtgt
      361 aggcgacaaa gtagtccaat gcaccttgta cagggtgaat gatatagccc ccaatcacag
      421 cttgtagaag tcctgagatc aatcccgtca ataagccgcc ggcaactccc cagcggtacg
      481 ccatcaagat aatcggcagc atcgatagcg tgacagatcc tccttgaggc attttgaata
      541 gtgtcaagtt atccaataca aatgataaag ctcctagaat ggcaacctcc aacattaact
      601 gaagtctttt acgatccaat tgtaactccc cctttttata gtcggaggga tacggaatga
      661 aaaaagcgtt ttgtctgcct cgaccgggca tcacagtcaa tcggcacgtt tgaattatgc
      721 tgatcatggc tacggttagt aaaacagctt cgaaactctt tcgcaacaaa aaaacgacac
      781 cccggataga accgagacat cgatcattgt cagtttccat ccacatccct acgcaagcat
      841 gaacttacag gttcgaaggg tcaggacaga tgtccaatct cagccattga aggcccccct
      901 tgtggtcatg taattcaatt gtcgtatcca ttgtatatgt tacagtactt atatgcaaga
      961 taagtttgta aactacttat tagaaccgcg ggaggtaaac tcatgacgaa tcataaacta
     1021 ttatccgccc tctgctactt cagcattttc ttttcgccgc tgttgctgcc tgcgatcgtc
     1081 tattttgtca cggatgatca tgaggtgaag gggcatgcga aacgttcgct catctcacat
     1141 cttgtaccgg tcgtactcct cattgccggc ttcatcctgt tttctttttc gatgatctcg
     1201 tatgaatcga tgatgcacac aatgatgaca ggacgattcg acttttgggg attggcgcca
     1261 tttctattca tgatcatcta tggcctgcta tttttattcg tcgtcatctg gaacgtctat
     1321 caaggcgtga aagtgttgaa atgataagca ggacaggcat acgtttattt acacatgaat
     1381 aattatgtta taatcagatg gaaaattacg tttcaaagaa gggacctgta aatcatgatt
     1441 gtgaagaaca acgaagagat cgaagcgttg aaaaagatcg ggcgcatcgt cgcagaggtg
     1501 cgggaagcga tgaaagcggc gactgttccg ggcattacga cgaaagagat cgatgaactc
     1561 ggaggacgcc ttctcgccga gcgcggagct gtgtcagcac cgattgacca atatgagttc
     1621 ccgggctata catgcatcag tgtcaatcat gaagttgccc acggcattcc gggatcacgc
     1681 gtcatccagg acggcgacct cgtcaatatc gatgtttccg catcattcgg cgggtacttt
     1741 gcagataccg gtatttcatt tgtcgtcggt acagccgatg aaaagaaaca actgctctgc
     1801 gatgtagcga aaaacgcttt tgaccgcgcg atgacaaaag tgaaagcagg ctcaagcttg
     1861 aaccagatcg ggaaggcagt cgagcgggaa gccagggata acggtctgaa ggtcattaaa
     1921 aacttgaccg ggcacggaat cggcacatct ctgcatgacg agccgcagca tattctcaat
     1981 tattatgatg catgggataa aacattgatg aaagaaggca tggtattggc ggtagagccg
     2041 ttcatctccg aaaaagccga gcacatcatt gaactaggag acggctggac gttcgtcacg
     2101 ccggacaaat ccctcgtcgc tcaaattgaa catacgatca tcgttacaaa agacgagccg
     2161 atcattttga cacaactata agaacaaaag cggagagtcg gctattgcca ctctccgttt
     2221 ttttatcatt gaatgattat taggcggtta tgacaagttt ttaggcggtc ctgttgttgc
     2281 atcaggcggt tctgaagagt ttttaagcgg tcctgttgtt gcatcaggca gttgccgcaa
     2341 ttatcaggta ctcctagggc gcgcgaaaag ttttgctcga gtttgaatgg aaaatgctcg
     2401 agttaggata cattatgctc gagttccagt aagttttgct cgagtttgac atcgttatgg
     2461 cgcaatctcc gacggttatg ctcgtcttat gactttagac acatcctata atgaaaataa
     2521 aggctgtcca gaaagccatt ctgactttct ggacagcccc tattttcatc attttttctt
     2581 cgttttcttc acgtcttgtt gaccgcttac cgtcttcgaa ttcccttcgg tcaacttccc
     2641 tttataccag actttttgga cgggcttgaa gccttttgta agctttctgt acgaccgttc
     2701 ttcaggatag gagagcaatg tgagcacttc cccatcttcc cctgcacgtc ccgtccgtcc
     2761 cgagcgatgt aaatattgct cgatcgtatg cggtacatcg acgtggatga catgggtcag
     2821 tccttgtata tccaatcctc ttgccgccaa atccgtcgct atcaaaatcc ggatttcacc
     2881 tttccggaag ttttccagcg tctcctgcct ttcgaattta gtcatcgaag agtataggac
     2941 cgcaacaggt gcctcattat attgcagctt catctccttc atccgaagct gatcgacatt
     3001 attcataaat gcgagcgccc tgattgaagg caggtgcgac agcccccgaa gcaggtctgt
     3061 cttatcccgt gcatccgtct tcacaaatga atggaccacc ttgcccgaca acggcatatc
     3121 ctcgacagag acatgcagac ggattggatc attcatcagt tttttcgcga caagttcaat
     3181 ttcatccgtg atcgttgcgg agacgacggc gacttgacgt tcgggattcg ccgcttcaat
     3241 cagatctttc acgatgacac gataatcccg tgacagcagc tgatcgcctt catccaatac
     3301 gatatagtgg atatcatgca tttttaaacg cttcgctttc acaagttcaa cgagacgccc
     3361 aggcgttccg acgacaatgg tcggcttctt tttcaaacgt tcgatctggc gttgcatgtt
     3421 cgcgccgccg atcaactggg tcaccgttat atcggttcct tccacccatt cacggatgac
     3481 gttgacaatc tgcatcgcca attcctggga aggagccatg atcaatgctt gcgtctgcat
     3541 ctttttgccg tccacttgct gaaggatcgg caagacatag gcgagcgtct ttcctgtacc
     3601 ggtcggtgat tccgctacaa catcccggcc attcaacaat tccgggatca tttgggattg
     3661 gataggtgtt tccctttcaa atttccactt attcttaatg ttatcgtcca ttttttccaa
     3721 gaaggacatt ctcatccccg ctttcactgt tccttatcta ataatggacc gaatccgttt
     3781 ttctttcaag tcaaggcttt ctccttgctt tcgcagactg ttcaaatgca taggcatagc
     3841 cgaccagttc aggttcggaa aacgccttgc cgcatagcgt aatgcaaaaa ggctcgcctt
     3901 cttcagtgaa ggatgatggg accgtgattg ccggatatcc cgccgccgca ctaaaactgc
     3961 atccatgatc ctgcgggaac acgagcacgt ctatcttgtg ttcgtcaaat gcccggtcaa
     4021 gagccaattc ccccgccaaa tgacggtttc tcaacaatgc ctcgatatat tccggctctg
     4081 tcagcgttcc actcgtccga tccactttct ccaacatcac ttgaccgaag tcaagcgtct
     4141 cttccggatg ctccttattg aattggatga tatcggataa cgtacggatc gggtttgtca
     4201 tcggcgtttt tccgaggtat tcattcaaag ccgctttgaa ttcatacaga aggacatcat
     4261 agcctaaatc gtcttccatc gtccccaaat cgatattgtc gacaagttcc gcacctaaag
     4321 aatcaaggcg gcgcagcgct tcttcgaact tctctttcct ttccgtcgaa atctcccgga
     4381 caaaaatcga acgtgcgatg ccgatccttt tgccttccag cgaagatgcg tcaagcattg
     4441 acagccagtc gactccctcg aatcggcggg cctccctcgt tacaggatcg gtttcatcat
     4501 gaccgaccat caatccgaat gtgatggctg cgtcttccac tgtccgagcg agcggtcccg
     4561 gtgtatcttg ggtgaatgaa agcggaatga taccgcttct gctaatggcc ccgacggtcg
     4621 gcttaatgcc gacgagggaa ttttgggcgg acgggttaat gatggatcca ctcgtttccg
     4681 tcccgatgga gaccgccgcc atattggctg caatagcggc cgcggcgcct gagctcgacc
     4741 ctcccacgtc aaaaaggcca tatggattat tcacttggcc gccgcgcgaa ctccagccat
     4801 tccgcatgcg atccgacatg aaattcgccc attcggtcat attggtcttg cctaaaatga
     4861 ctgcgcccgc atcccttaac ttctttacta aaaaggcgtc ctcttttgca taatagtttt
     4921 tcatcgccaa cgagccgcag ctagtatgca tgccatcgcc agtatccata ttgtctttca
     4981 acagtaccgg tatgccatgc aataatgaac gcacaatccc gttgcgccgt tctgcgtcta
     5041 gcgacctggc gatttgcaag gcttgaggat tcagctccag cacagcattg atattccggt
     5101 ctctctctga aattatcttc atgtacatga cagtcagttc ttccgaagta agacggccgg
     5161 tagacatccg ctcctgcatt tgccggattg ttgcctcctc cagccattca aattgcatct
     5221 catccagttt cctgttcaag caaatccccc catagtcttc actctatcct cattgtatac
     5281 tgtttcatca actcatataa agatagttca tataattgtt ttccgtttat tttaaagacg
     5341 ccttcattca caaggttttc gattatttta tcccttttgt tttcgagcac gggattttcc
     5401 tgctttttca tggtgttagt tcctcctaaa ttgttggcta tcatctattc cacttccatg
     5461 aaagctgtaa actatgaaga attaacca
//
LOCUS       DASULI010000281         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1217591,
            whole genome shotgun sequence.
ACCESSION   DASULI010000281 DASULI010000000
VERSION     DASULI010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217591"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFX34_05995"
     CDS             <1..223
                     /locus_tag="VFX34_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5564491.1"
                     /translation="YYGKITTPVCVVQGKKDGIVPFSAGEHLLRSLGSEQKQLIVSEN
                     GKHHICYSDDSHEWFPKVYEFMKKELDEF"
     gene            581..2065
                     /locus_tag="VFX34_06000"
     CDS             581..2065
                     /locus_tag="VFX34_06000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5564492.1"
                     /translation="MTKFSELNISESTQNALARMGFEEATPIQEGTITFGMEGRDVIG
                     QAQTGTGKTAAFGIPIIEKVDVNNPAVQALVIAPTRELAIQVSEEIYKIGYGKRARVL
                     TVYGGQEIGRQIRALRNNPQIVVGTPGRILDHINRKTLKLNNVNTLVLDEADEMLNMG
                     FIEDINTILAAVPAERQTLLFSATMPPAIRKIAETFMKEPEMVKIKSKEMTVENIDQF
                     FVKAQEREKFDVLSRLLNVHQPELAIIFGRTKRRVDELSHALSIRGYLAEGIHGDLTQ
                     AKRMSVLRQFKDNKIDILVATDVAARGLDISGVTHVYNFDIPQDPESYVHRIGRTGRA
                     GKSGMAITFVTPREMGYLRVVEETTKKRMTPLQPPTEDEALIGQQRLAMEQLTEIIEK
                     NELTDYKPLAAELLEKFDAQDIVAAAIRSLTKEPDDTPVKITEERPLPSRKTGSRDRG
                     GYKGSRGGGRSYGGGRGYGGGGGSRRGSRDGGGRRDRSRGKSER"
BASE COUNT          627 a          489 c          538 g          525 t
ORIGIN      
        1 gtactacgga aagattacga ctcctgtctg cgtcgtgcag gggaaaaagg acggcatcgt
       61 cccgttttcg gcaggagagc atttgttgcg ctctctcggc tccgagcaga agcagctgat
      121 cgtatcggaa aatggaaagc atcatatttg ctacagtgac gactcccatg aatggttccc
      181 gaaggtgtat gaattcatga agaaagagtt ggacgaattc tgattatttc ttttaaagcg
      241 gattgattgc ggatgaccgg caggcgtgat aaactactat gagtattgga tacatttaca
      301 caagactctt ccggtggcga agagtacttt tttttgaata aaacggttta gcgtctcccg
      361 gagcaccatc ccggagcgaa acctgatcgg caaagaccag ctttcctttt cactattgcc
      421 tctgaaattc atgaagttca gccctttgaa cttttgccca ctccagagaa tacaaaatat
      481 cggaaacgtt cccatagaag ttaaaggctc gaatttgccg tgctttcatt tgcaaatgta
      541 accaattgct aaatgaagaa accaaaggag attgagaagt ttgacgaaat tttcagaact
      601 taatattagc gaatccacac aaaatgctct agcgcgcatg gggtttgaag aagcaacacc
      661 gatccaagaa ggcacgatca catttggcat ggaaggccgc gatgttatcg gtcaggcaca
      721 gacaggtact ggtaaaactg ctgctttcgg tattccgatc attgagaaag tcgatgtgaa
      781 caatcctgca gtacaagcac tagtcattgc acctacacgt gaattggcga ttcaagtatc
      841 cgaagagatc tacaaaatcg gttacggcaa acgcgcacgt gtgttgactg tgtacggcgg
      901 ccaggaaatc ggtcggcaaa ttcgagcttt acgcaacaat ccacaaattg ttgttggaac
      961 tccgggacgt attcttgacc acatcaaccg taaaaccttg aaattgaaca atgtgaatac
     1021 gctcgtcctt gacgaagcgg atgaaatgct caacatgggc ttcatcgaag atatcaatac
     1081 gattttggca gccgtaccgg cagaacgcca aacattactg ttctctgcaa caatgcctcc
     1141 agcaatccgt aagattgcgg aaacattcat gaaagagccg gaaatggtga aaatcaagtc
     1201 gaaagaaatg acagttgaaa atatcgatca gttcttcgtt aaagcgcaag aaagagaaaa
     1261 gttcgatgtc ctttcacgtt tgttgaacgt tcaccaacct gaacttgcga tcatctttgg
     1321 acgtacgaag cgtcgtgttg atgagctttc gcatgcatta agcatccgcg gttaccttgc
     1381 tgaaggaatc cacggcgatt tgacgcaagc gaagcgtatg tcagttcttc gccaattcaa
     1441 agataataaa attgatatcc ttgtcgcaac agacgtagct gcacgcggat tggatatttc
     1501 tggtgttaca cacgtttaca acttcgatat tccacaagat ccagaaagct atgtacaccg
     1561 tatcggtcgt acaggtcgtg ctggtaaaag cggtatggcg atcacttttg tcactccgcg
     1621 tgaaatgggt tatctccgtg tcgtagaaga aacgacgaaa aaacggatga ctccacttca
     1681 gccgccgact gaagatgagg cattgatcgg tcagcaacgc cttgcaatgg aacagctaac
     1741 cgaaatcatc gagaaaaacg aattgactga ttacaagcca ttagctgcgg agcttctcga
     1801 gaagttcgac gcgcaagata tcgttgcggc tgctattaga tcgttgacga aagaaccaga
     1861 cgatactcca gttaaaatta cggaagagcg cccgttgcct tcccgtaaaa cgggctcacg
     1921 tgatcgtggc ggctacaaag ggagccgcgg aggcggacgt tcttatggcg gcggtcgcgg
     1981 atatggcgga ggcggcggtt cacgtcgcgg aagccgtgat ggtggcggac gcagagatcg
     2041 ttcaagaggc aaatcggaaa gataatacac acccccggag agatgaaact tcatccccgg
     2101 ggttttttgc gtctagactc cataagtcaa agctgctcag tggcaaagtt cgccacgccg
     2161 cattttcgtc ttatgcctg
//
LOCUS       DASULI010000282         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1435974,
            whole genome shotgun sequence.
ACCESSION   DASULI010000282 DASULI010000000
VERSION     DASULI010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435974"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFX34_06005"
     CDS             <1..377
                     /locus_tag="VFX34_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564493.1"
                     /translation="GDEIRSVLQNPPTVGDIIASLDHSNQPTADITFNGAPVTITNSG
                     ASILLNGKQVDNSVTVNPGDRLTIPENAQRPFLFSDVFLFIDYSLPKNASSSYQLLCN
                     GEPIGFNDPIFSGDALEIKFNS"
     gene            complement(439..1077)
                     /locus_tag="VFX34_06010"
     CDS             complement(439..1077)
                     /locus_tag="VFX34_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX5564494.1"
                     /translation="MTDKLKPNYNDAHYDHRYYGNQEYKNTFGEPSHLPVNYPYRSNT
                     DDFYEDDDSGAGSFLLGALVGGVIGAAAALFLAPKTGREMRDDFSEQAVQLKNKGIEL
                     SAVAKDKATEITSVAKEKTDGLTKTIQEQSGQIVDKVKSMSNKTSVPMDDGTAFSDGE
                     EAIDFTEPTTKEDKKAAQDQSTKNSGVSYDNSENIGEEKNVNQNHISVEEEK"
     gene            complement(1110..1595)
                     /locus_tag="VFX34_06015"
     CDS             complement(1110..1595)
                     /locus_tag="VFX34_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX5564495.1"
                     /translation="MEVLLYIAAIVAAIAFLILCISLAMTLGSLKTTLRSVSETLDGL
                     TNQLEGVTSETTELLHKTNALAEDIQQKSEKLNTVVDAVKGVGSSVNDLNDSVRRVTH
                     SITVEAEKNSDKIAQVVQWSNVALGIIGKVKDMKTERSSGWTVYQPDKGQKRLPSPDG
                     H"
     gene            complement(2047..>2245)
                     /locus_tag="VFX34_06020"
     CDS             complement(2047..>2245)
                     /locus_tag="VFX34_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX5564496.1"
                     /translation="SAREKSGNLTIDDLVSKIPGAKHLELENIDVLDEHGNAVYLFMG
                     AGDIQKYLNVFAEHTKKEETA"
BASE COUNT          537 a          547 c          459 g          702 t
ORIGIN      
        1 acggtgatga aatcaggtca gtgctgcaaa atcccccgac cgtcggggat ataattgctt
       61 ctctggatca ttcgaatcaa ccgaccgcgg acattacgtt caatggggca cctgtaacga
      121 ttacgaacag cggagcatcc attttattga acggcaagca agtggataat tccgtgactg
      181 tcaatccagg agaccggtta acgattccgg aaaatgctca acggccattt ttgttcagcg
      241 atgttttcct atttatcgat tattcattgc cgaagaatgc ttcatcttcc tatcagctgc
      301 tatgcaatgg ggagccgatc ggctttaatg acccgatctt cagcggggat gcattggaaa
      361 tcaaattcaa ctcctgatat tacatgttta taagcaaaag ccagacaacc caataagggc
      421 tgcctggctc ttttgatttc atttctcttc ttcgaccgaa atatgatttt gatttacatt
      481 cttttcttca ccgatgttct cgctattgtc ataggagaca ccactgtttt tcgtcgattg
      541 gtcttgagct gcttttttat cttcctttgt tgtcggctct gtaaaatcga ttgcctcttc
      601 accatcggaa aaagctgtcc catcatccat cggcactgat gttttattcg acatagattt
      661 cactttatct acaatctggc cggattgctc ctgaatcgtt ttcgttagac catccgtctt
      721 ttccttggcc actgaagtga tttccgtcgc tttatccttt gcaactgcac tcaattcgat
      781 tcctttgttt ttcaattgca ccgcttgctc cgaaaaatcg tcgcgcattt ccctgcctgt
      841 tttcggtgcc aggaacaatg cagcagcagc gccgatgact cctcctacaa gggcacctaa
      901 taggaagctt cctgcgccgg aatcgtcatc ttcatagaaa tcgtccgtat tggaacggta
      961 tgggtagttg acaggcaagt gggatggttc cccaaaagtg ttcttatatt cttggttccc
     1021 gtaataacgg tgatcataat gtgcatcgtt atagttcggt ttaagtttat cagtcatgtg
     1081 aattcctcca gtatgatttt ttggcgatat cagtgcccgt caggacttgg caatctcttc
     1141 tgtcccttat ccggttgata gacagtccag cctgatgatc tttccgtctt catatctttc
     1201 actttaccga tgataccgag tgcgacgttg ctccattgaa caacttgggc tattttatca
     1261 ctgttcttct cagcttcgac agtgattgaa tgggtcactc ggcgaactga gtcgttcaag
     1321 tcgttgactg agctccctac ccctttcact gcatcaacga cagtgttcaa tttttcggat
     1381 ttctgttgaa tatcctccgc aagggcattt gtcttatgaa gaagttcagt cgtttccgac
     1441 gtgacccctt ccaactggtt cgtcaagcca tccaatgttt cggagacgct tcgtaacgtt
     1501 gttttcaatg aacctaatgt cattgcaagg ctgatacata aaatcaaaaa tgcgattgcc
     1561 gcaacgattg cagcaatgta cagtaaaact tccatggcaa tcctcctccc gataacatcc
     1621 ataagtaatt tctacccttt aaatgaggta aataaacgcc gcctactcta acctttttcg
     1681 acatcttttg caaatctcct tcatttttcc cgaaataaat tatttctgga tattaagata
     1741 gagattttac atttgattgg aataacgaaa attgccagca cggctccctt tccgtgagtg
     1801 tcgcctcagc caatcgaaca gcgaagggtt cgatttgccg tatttctgcg ttttgtgcag
     1861 atttaaggca tcctgctccc ttaaggtcgc atcaaacatt gctcccaacg cttcgctttt
     1921 ggtcgcaaaa gacgcagtgt tgcgagcacc ttcgcgaagg aagtagctca agccacgccc
     1981 ccggaaagcg tccgtctgaa acggaaatca actttttttc tataaaaaaa cagccgatcc
     2041 atgggatcag gctgtttctt ctttttttgt atgttcggcg aagacgttta aatacttttg
     2101 gatgtcgcca gcgcccatga aaaggtaaac tgcgtttccg tgctcatcga gaacgtcgat
     2161 gttctccaat tcgagatgct ttgctccggg gattttgctg acgagatcgt caatcgtcaa
     2221 attgcctgac ttctcccttg cggaa
//
LOCUS       DASULI010000283         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_547584, whole
            genome shotgun sequence.
ACCESSION   DASULI010000283 DASULI010000000
VERSION     DASULI010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547584"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFX34_06025"
     CDS             complement(<1..313)
                     /locus_tag="VFX34_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5564497.1"
                     /translation="MARRIRYWNPYGYFHVTMRGNNRQAIFQTEEDKLNLMRAFEYAY
                     DKYTFSMVAYCIMPNHYHLLIQSEVELSSVMARINRRYSDYYAKRYDHVGPIYEKRCY
                     SK"
     gene            complement(315..554)
                     /locus_tag="VFX34_06030"
     CDS             complement(315..554)
                     /locus_tag="VFX34_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564498.1"
                     /translation="MVIMSVGLQGMKGHKVAVEASVRVSRNQFVIVGSKLIRIARTIT
                     DSEGLELIPENTVQEAIKWKSMAPGLHRPAVMEGE"
     gene            1228..>3241
                     /locus_tag="VFX34_06035"
     CDS             1228..>3241
                     /locus_tag="VFX34_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="X-prolyl-dipeptidyl aminopeptidase"
                     /protein_id="HEX5564499.1"
                     /translation="MKRFLVGFLALFMAFATAVPLSAQAIQSVETVQVADTVELDVSR
                     SILSLTEIRDVTIEADFGREVDLDNLELQFGGKALDEWKQWDAAARSYSGKPFILVKK
                     EPHYVEGTTKIAAELEFGLPFGTDDLSPRSIRVLYKELLGDYELALVDSENEVKAAAT
                     VELNVYDEFQEWDEIKPTIDQIFEEAHGLNDRYLHYESPGKSVEGEDMHLVVLAKDED
                     AVNNYLGNTLPAALETPEDLLAKLKEGKMGDYQIPIWFNNIHPDEAEGIDFQTGLLKK
                     FALESEVTFTTTNENGIDEEVVLNVDEVLDHLIFIFNFSHNPDGRVHNTRANVNGFDL
                     NRDNAYQTQVETIHLNQVIAKWTPLAMMEGHGYVKGFLIEPATPPHNPNFEYDLLMEG
                     MIPQAHAMGQAGIGSSRLTSYFLASEEYEDGWDDMGPSYTPIFSMLHGTLGHTVEVPT
                     LSQDAYYAMVGAGLGMINYVFENKDDLYARQLEIFDRGVKGEDNRAVDPLLTNAKGES
                     IGRDRGEHENFFPDYYVLPTDSTNQKNVSEVYNMVEYLIRNGVKVERTTKDVKIGQVT
                     YPKGAYIVPMKQAKRGLANAMLYKGDDVSDWNAMYDPVVVNFPALRGFDVVDVRMGGE
                     DVLEGLTEQVTEVTMPSSEVDGKKAMQVLKNVNNDTIRLVNELLQDG"
BASE COUNT          896 a          685 c          822 g          838 t
ORIGIN      
        1 atttggaata acaccttttt tcatagatgg gcccgacatg gtcatagcgt ttggcatagt
       61 agtcactgta ccttcggttg atgcgtgcca tgacgctgct caattccact tcggattgga
      121 tcagcaagtg atagtgattg ggcatgatgc aataggcaac catggaaaaa gtatatttgt
      181 cgtacgcata ttcgaaggcg cgcatcaaat tcaacttatc ttcctcggtt tgaaagatcg
      241 cttgacgatt gttgccccgc atcgtcacgt ggaaataccc atacggattc caatagcgta
      301 ttcttctcgc catatcattc accctccatg actgctggtc tatgcaaccc gggtgccatc
      361 gacttccatt tgattgcttc ctgtacggtg ttttccggga ttagctccaa tccttccgaa
      421 tccgtaattg tccgcgcgat gcggatcagc ttggacccga caatgacgaa ttgattccga
      481 ctcaccctca cacttgcctc aacagcaacc ttatgccctt tcatcccttg cagcccgaca
      541 ctcataatta ccattgcgat tgcacatcac cactttcttt ttctccaacc atatcctctc
      601 gtgaatagac tgacaactgt ccgaattcaa cagaaaacgg aaaaatggat ttttcacgcg
      661 gaaatcgggt cgaaattgat ttttaagcca ggataagcag ggaaaataat attttcagtg
      721 gatttttcat attctcgaaa agttttttgg gtgcctgtgt aacaaactat tccgacaatt
      781 cactacaatt gaataaccaa atacataaac ggcttaattt caacaatcca tattctaatt
      841 catgcattaa tattcccata actgaatagt cataaatgtg tcttgcacag gcactggcac
      901 aattctgaca atacactaca attgcataat agtttaacag gaagtcagct atatccaact
      961 ttggctggct tcttttgtca tttatataca tctcattgaa tagtcataaa tgcaccttgc
     1021 acaggcaccg atgaaagagg aaccaattac cggtgaattg tatggaattt ctaaatgttt
     1081 ggtgatcttg tcctgggtgg tttggggcat tttgaggatt acttcagatg tcgaagtaaa
     1141 tcgctgtcta tctatctgtc aatttgatat agtcgcatta ttgtagacta ttatcctgca
     1201 atacaaaatt gtagagggga tgagatgatg aaacggtttt tggttggatt tttagcattg
     1261 ttcatggcgt ttgcgacagc ggttccgctg tctgcgcaag ctatccagtc ggttgaaaca
     1321 gtccaagttg ccgatacggt tgaactggat gtcagccgct cgatattgtc gctgacggaa
     1381 atacgcgatg tgacgataga agcggatttt ggaagggagg tcgatttaga caacctggaa
     1441 ctgcaattcg gcgggaaagc actcgatgaa tggaagcaat gggatgcggc agcaagatcg
     1501 tattccggga agccattcat ccttgtgaaa aaagagcccc attatgtgga gggaacgacg
     1561 aaaatagcag cggaacttga attcggattg ccgttcggaa cggatgatct ttcacctcgc
     1621 tcgatccggg tgctttacaa agaattgctc ggggactatg agcttgcgtt agtagactca
     1681 gagaatgaag tgaaagctgc tgcgacggtc gagttgaatg tgtatgacga attccaggaa
     1741 tgggatgaaa tcaagcctac catcgaccaa attttcgaag aggctcatgg attgaatgac
     1801 cgctatctgc attatgaaag tccagggaag agtgtggaag gcgaagatat gcaccttgtc
     1861 gtccttgcga aggatgaaga tgcggtgaat aattatttgg gaaacacttt gcctgctgcg
     1921 cttgaaacac ccgaggattt attagcgaag ctgaaagagg ggaagatggg agattaccag
     1981 attccgattt ggttcaacaa tatccacccg gatgaagcgg aaggaatcga tttccaaacc
     2041 ggattgttga agaaatttgc attggaatcg gaagtcacct tcacgacgac gaatgaaaat
     2101 gggatagatg aagaagtcgt gttgaatgtc gatgaagtgc tcgaccatct cattttcatt
     2161 ttcaacttct cgcataaccc ggacggccgt gtccacaata cgcgggcgaa cgtgaacgga
     2221 ttcgacttga accgagacaa tgcctatcag acgcaagtcg aaacaatcca tttgaatcaa
     2281 gtgattgcaa aatggacgcc gctagcgatg atggaagggc atggttatgt aaaagggttc
     2341 ctcatcgagc ctgcgacacc gccgcataat ccgaacttcg aatatgatct tttaatggaa
     2401 gggatgattc cgcaggcgca tgcaatggga caggcgggca tcggcagctc aaggctgacg
     2461 tcgtacttcc tcgcctccga agagtatgaa gacggctggg atgatatggg accttcctat
     2521 acaccgattt tctcgatgct gcacggaacg cttggccata cagtcgaagt gcctacgctc
     2581 agccaggatg cctattatgc gatggttggg gcaggcctcg ggatgattaa ttatgtgttt
     2641 gagaataaag atgatctgta cgcacgtcag ctcgagatct tcgaccgtgg cgttaaaggg
     2701 gaagataatc gcgcggtgga tccgctattg acgaatgcga agggtgaatc gatcgggcga
     2761 gaccgcggag agcatgaaaa cttcttccct gattattatg tgctcccgac cgactcaacg
     2821 aatcagaaga acgtgtcgga agtgtacaat atggtggagt atttgatccg caacggtgtg
     2881 aaagtcgagc ggacgacgaa agatgtcaaa atcgggcagg ttacgtaccc gaaaggtgcc
     2941 tacatcgtac cgatgaaaca ggcgaaaagg ggcttggcga atgccatgct gtacaaaggc
     3001 gatgacgtct ccgactggaa tgcgatgtat gatccggtcg ttgtcaactt cccggcactg
     3061 agaggattcg atgtcgtcga cgtgcgcatg ggtggggaag acgtacttga agggctgacc
     3121 gagcaagtga cggaagtgac gatgccttcg agtgaagtgg atggtaagaa agcgatgcaa
     3181 gttttgaaga atgtcaataa cgacacgatc aggttggtga atgaactgct gcaagacggt
     3241 a
//
LOCUS       DASULI010000284         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_372333, whole
            genome shotgun sequence.
ACCESSION   DASULI010000284 DASULI010000000
VERSION     DASULI010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372333"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="pnp"
                     /locus_tag="VFX34_06040"
     CDS             <1..1129
                     /gene="pnp"
                     /locus_tag="VFX34_06040"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5564500.1"
                     /translation="GILQRTHGSGLFTRGQTQALSVCTLGALGEVQIIDGLGLEESKR
                     FMHHYNFPNFSVGETGPIRGPGRREIGHGALGERALEAIIPDEKEFPYTIRLVAEVLE
                     SNGSSSQASICASTMAMMDAGIPIKAPVAGIAMGLVKKGDNYSVLSDIQGMEDHLGDM
                     DFKVAGTEKGITALQMDIKIEGLSRQILEESLAQAKIGRMHILNHMMATISAPREELS
                     QYAPKIIMIRINPDKIRDVIGPGGKVINKIIEETNVKIDTEQDGTIYISSPNSEMNEK
                     AKTMIENIVREAKAGEYYLGKVKRIEKFGAFLEIFPGKDGLLHISEIQEERTKAVEDV
                     LKMGDELLVKVIEIDNQGRVNLSRKVVLKEEKEAAEKGKNE"
     gene            1221..2462
                     /locus_tag="VFX34_06045"
     CDS             1221..2462
                     /locus_tag="VFX34_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5564501.1"
                     /translation="MQKKICQNGVRIIHEKMPYVRSLAIGLCVKAGSIDETEKEWGLS
                     HFIEHMLFKGTTTRSARMIAEQFDRIGGDINAYTSKEMTCYYATVLDHHAEEALTILA
                     DMFFNSSFNAGDIQKEKSVILDEIASVEDTPDDDVDERLWSVMYPQHTVGRSIGGIKE
                     TIDSFDKVMIEQYISKHYTAENIVISIAGNYDEELIGFIENLFTSFTLKESSDSSVAG
                     RPVFHSGRTTKSKEIEQAHICIGYPGLTLTDERLHDLILLDSIVGGTMSSRMFQKIRE
                     EKGLAYSVFSYYSAYTSTGSFVIYGGSGPEHLEEMVNTMDGIISDIMANGVTVKELHN
                     AKEQLKGSFLLGLESSESRMHRNGKNELILLEHKTIDEVVDLIDKVQLENVNLIARET
                     FARERAISIIAPKKAVKRIRL"
     gene            2529..2804
                     /locus_tag="VFX34_06050"
     CDS             2529..2804
                     /locus_tag="VFX34_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HEX5564502.1"
                     /translation="MLLSEMAQKELIQVEEGIRYGFLADTDLIFNRKTGAIIGFEVKK
                     KFGRFPFRNREQETSEFIPWSEIVLIGEHRILFGKTHSLGEFDIDDN"
     gene            2791..>2978
                     /locus_tag="VFX34_06055"
     CDS             2791..>2978
                     /locus_tag="VFX34_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564503.1"
                     /translation="MTTIDGKWLFIGTDRRLSECSRIMGDRGFDVLHYEGNGYSEELS
                     DILDEISPAHIVFPILQM"
BASE COUNT          898 a          583 c          764 g          733 t
ORIGIN      
        1 cggcatttta caacgaacac atggttccgg cttgttcact cgcggacaaa ctcaagcact
       61 tagcgtatgt acacttggag cccttggaga agtacagatc attgacggcc ttggacttga
      121 agagtcgaag cggttcatgc atcactataa cttcccgaac ttcagcgtag gggaaacagg
      181 accgatccgc ggaccgggcc gtcgtgaaat cggccatggc gctctcggtg aacgtgcgct
      241 tgaagcaatc attccggatg agaaagagtt tccatacacg atccgtctcg tagcggaagt
      301 attggagtcg aacggatctt cctcccaagc gtcaatctgt gcctctacaa tggcaatgat
      361 ggatgcggga atcccgatca aggcaccagt tgcgggaatc gcaatgggac ttgtgaaaaa
      421 gggggacaat tattccgttc tttcagatat ccaagggatg gaagaccatc ttggcgatat
      481 ggactttaaa gttgccggaa ctgaaaaagg gattaccgct ttgcaaatgg atatcaaaat
      541 cgaaggatta tcccgtcaaa ttctagaaga gtccttggct caagcgaaaa ttggccggat
      601 gcatatactg aatcatatga tggcgaccat ttctgcgcct cgtgaagaac tttcacaata
      661 tgcgccgaaa atcatcatga tccggatcaa tcctgacaaa atccgtgatg tcatcggacc
      721 tggcggtaag gttatcaata agatcatcga agaaacgaat gttaaaatcg atacggaaca
      781 agacggaaca atctacattt cttcccctaa ctcggagatg aacgaaaaag cgaaaacgat
      841 gattgaaaac atcgtccgcg aggcgaaagc cggagagtat tatttaggta aagtgaaacg
      901 gattgagaaa ttcggcgcgt tccttgaaat attcccaggt aaagacgggc ttctccatat
      961 ttccgaaatt caggaagaac gtacaaaggc tgttgaggat gtattgaaga tgggcgatga
     1021 acttcttgtc aaagtcattg aaattgacaa tcaaggtcgc gtgaatctat cccgtaaagt
     1081 cgttttgaaa gaagaaaaag aagcggcgga aaaagggaaa aacgaataac gatttgcata
     1141 tgataatgga aagcccctct ttacgatggc gtgaagatgg tggctttttg ttatttcagt
     1201 tagagggggg aaaagaattg atgcaaaaaa agatctgcca aaacggcgtg aggatcattc
     1261 atgaaaagat gccatatgtc cgctctttgg caatcggcct atgtgtgaag gccggttcca
     1321 tagatgagac ggaaaaggag tgggggcttt cccacttcat tgaacatatg cttttcaaag
     1381 ggacgacgac gcgcagcgca agaatgatcg cagagcaatt cgaccggata ggcggggata
     1441 ttaatgcgta cacttccaaa gagatgactt gctattatgc gaccgtgctt gatcatcatg
     1501 cggaagaagc gttaacaata ttagcggaca tgtttttcaa ttccagtttt aacgcgggag
     1561 acatccagaa ggaaaaatca gtcattttgg atgaaatcgc atccgtagaa gatacacctg
     1621 acgatgacgt tgatgaacgt ctttggtcag tcatgtaccc gcagcatacg gttggaaggt
     1681 cgataggcgg gattaaagaa acaattgatt catttgataa ggtaatgatc gagcagtaca
     1741 tatcgaagca ctacacagct gaaaatatcg tcatttcgat tgctggaaat tatgacgaag
     1801 aattgattgg attcatcgaa aatctgttca cttcattcac actgaaagaa tcatctgatt
     1861 ccagtgtggc gggccgccct gtcttccaca gcggcaggac aacgaaatcc aaagaaatcg
     1921 aacaagcaca tatttgcatc ggttacccgg gtcttacgct gacggacgaa cgtctgcacg
     1981 atttgattct gcttgatagt atcgtgggcg gaacaatgtc ttcacggatg ttccagaaga
     2041 tccgggagga gaaagggctt gcttattccg tgttctccta ttattcagcc tatacatcaa
     2101 caggctcatt cgtcatttat gggggaagcg ggcctgaaca tttggaagag atggtgaata
     2161 cgatggatgg catcatcagt gacattatgg cgaatggagt gacggtgaag gagttgcaca
     2221 atgcgaagga acaactgaaa ggcagctttc ttctcggcct tgaaagttcg gaatctagaa
     2281 tgcaccgtaa cggcaagaat gagctgattc ttttagagca taaaacgatt gacgaagtag
     2341 ttgacctgat cgataaagta caattggaaa atgtcaattt gattgcgcgc gagacgttcg
     2401 cccgggaacg ggcgatttca atcatcgccc cgaaaaaagc ggtgaaaaga atccgtcttt
     2461 agaaacgtgt cagctttgcc tcctgcgaat catatgataa gtcaggacat ttaaaagggg
     2521 gcaaatatat gctgctgtct gaaatggcac agaaagaact tatccaagtg gaagaaggaa
     2581 tccggtatgg atttcttgcg gatacggatt tgattttcaa tcggaaaaca ggagcaatca
     2641 tcggcttcga agtgaaaaag aaattcggac gatttccatt ccgcaatagg gaacaggaaa
     2701 cctcggaatt cataccgtgg agtgaaatcg ttttgattgg ggaacatcgg attctttttg
     2761 gaaaaacgca tagtctgggg gaatttgata ttgacgacaa ttgacggaaa atggctgttc
     2821 attggaacgg accgaagatt atcggaatgc agccggatta tgggggatcg tggcttcgat
     2881 gtgcttcatt atgaagggaa cggctattct gaagagttgt ccgatatcct tgatgagatt
     2941 tctccggccc atatcgtttt tccgattttg caaatgga
//
LOCUS       DASULI010000285         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_668090, whole
            genome shotgun sequence.
ACCESSION   DASULI010000285 DASULI010000000
VERSION     DASULI010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668090"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFX34_06060"
     CDS             <1..320
                     /locus_tag="VFX34_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5564504.1"
                     /translation="AFLVSIADSLPLLCIGLFLIPMAAFFLYSNQLFIGVSLALLYIF
                     VVMTRQLAESYMWASAFRVKPIHAFFITVCSLYLFGLPGILLTPFLLFTALKIKKHPV
                     FTG"
     gene            complement(310..693)
                     /locus_tag="VFX34_06065"
     CDS             complement(310..693)
                     /locus_tag="VFX34_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX5564505.1"
                     /translation="MKWLLLLFILVPAAEIMLLLYSGNTIGIMPTLLLILISGIGGAY
                     LAKRQGFKAFMDLRNRMGTMEAPGNAVIDGVCIFFGAILLIMPGFITDVAGLLLLFKG
                     PRNIIRPFIVNWIYKKMKQGRIVIR"
     gene            complement(765..>2506)
                     /gene="pyk"
                     /locus_tag="VFX34_06070"
     CDS             complement(765..>2506)
                     /gene="pyk"
                     /locus_tag="VFX34_06070"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5564506.1"
                     /translation="CTIGPASESPELLEQLIEAGMNVARLNFSHGTHEEHKARIKSIR
                     EASKKTGKVVGILLDTKGPEIRTHSMKNGQVELITGHHIDISMTEVEGDDNVFSVTYE
                     KLIEDVDKGSVILLDDGLIQLEVVGKDLEKGLIHTIVINSGTLKNKKGVNVPGVSVQL
                     PGITEKDKEDILFGIREGVDFIAASFVRRASDVMEIRELLERNDGGHIHIIPKIENGE
                     GVDNLDDILTVSDGLMVARGDLGVEIPAEEVPLVQKKMIEKCNQVGKPVITATQMLDS
                     MQRNPRPTRAEASDVANAILDGSDAIMLSGETAAGLYPVESVRTMDRIAKTAEDAVDY
                     RSVVSTRRREKHGNMTESIGQAVAYTAINLKVKAVLAPTESGHTAKMIAKYRPGCPII
                     AITASEECSRKLSLVWGIYPIIGERVHSIDEILQESVEESVKHQYVTHGDVVIITAGV
                     PVGEAGTTNLMKIHVIGDMLAKGQGIGKSVAFGQAVVAHNAQDALARDMKGKILVTYS
                     SDRDMMPAIEECAGLITEEGGLTSHAAVVGLSLGIPVIVGVEKATELIVDGKDITMDA
                     ESGVIYNGHARVL"
BASE COUNT          595 a          669 c          510 g          732 t
ORIGIN      
        1 gtgcattcct tgtttccatc gccgatagtc tcccgttgtt gtgcatcggt ctattcttaa
       61 ttccgatggc ggccttcttc ctttattcca accaattatt cattggtgta tcgctcgcct
      121 tgctctatat ttttgtcgtt atgaccaggc aattggctga atcatatatg tgggcttccg
      181 catttcgcgt aaagccgatc catgcatttt tcatcactgt ttgctcactc tatctgttcg
      241 ggttgcctgg tattctgtta accccatttt tacttttcac ggcattgaaa ataaaaaagc
      301 atccggtatt caccggatga caattcgacc ttgcttcatt ttcttgtata tccaattgac
      361 gataaacggc ctgatgatat ttcttggccc tttgaataaa agcaaaagcc cagcaacatc
      421 agtaataaag cctggcatga tcaataaaat agcaccaaag aaaatgcaga caccatcgat
      481 tactgcattt cccggggctt ccattgtccc catcctgttc cgcagatcca taaaagcttt
      541 aaatccttgc ctttttgcaa ggtaagcccc tccaattccg ctgatgagaa taagcaggag
      601 ggtcggcatg attccgatcg tgttgcctga atagagcagg agcatgattt ccgcggctgg
      661 tacgagaata aataacagca atagccattt cattccactg caactccaat cctaaaaagg
      721 ctgcttccgc aacaagtcgg aagacagcct catagcgtta ttttttatag tactctcgcg
      781 tgtccattgt aaatgactcc ggattccgca tccattgtta tatctttgcc atctacaatt
      841 aattcagtcg ctttttctac tccgacgatg acggggattc ccaagctgag gccgacgaca
      901 gccgcatgac tagtcaaacc gccctcttcc gttattaagc cggcacattc ctcgatggca
      961 ggcatcatat cacggtccga agaataggtg acgaggatct tccccttcat atcacgcgcc
     1021 aatgcatcct gggcattatg tgcgactacc gcttggccga acgcgaccga cttaccgatt
     1081 ccctggcctt tggcaagcat atcgccaatg acatggattt tcatcaagtt cgtcgttcct
     1141 gcctcaccga ccggtacgcc agcagtgatg atgacgacat caccatgggt gacgtattgg
     1201 tgcttcacac tttcctctac ggattcctgc aaaatctcat cgatggaatg cactctttcg
     1261 cctatgatcg gatagattcc ccatacaagc gatagcttcc tcgaacattc ttccgaagcg
     1321 gtaattgcga taatcgggca tcccgggcga tatttggcaa tcatttttgc agtatgcccg
     1381 ctttcagtag gggcaagcac ggctttcact ttcaaattga tagccgtata agctacggct
     1441 tgaccgatcg attccgtcat attgccatgt ttttcacggc ggcgtgtgga aacgacagaa
     1501 cggtaatcga cggcatcttc agccgttttt gcaatccggt ccatcgtacg gacagattca
     1561 accggataga gtcccgccgc tgtttcccct gaaagcatga tcgcatcgga gccatccaaa
     1621 atggcatttg ctacgtcact tgcttcagct cgtgtaggac gtggattccg ttgcatcgaa
     1681 tcaagcatct gcgtcgctgt gatgaccggt tttccaacct ggttgcactt ctcaatcatt
     1741 ttcttttgga cgagcgggac ttcctccgcc gggatttcca cgcctaagtc gccccgtgca
     1801 accatcaagc cgtcggatac cgtcaagatg tcgtccagat tgtcgacacc ttccccgttc
     1861 tcgatcttcg gaatgatatg gatatggcca ccatcgttcc tctccagcaa ttcccggatc
     1921 tccataacgt ctgaagctct tcgcacaaaa gaggccgcaa tgaaatctac gccttcccta
     1981 atcccaaata gaatgtcctc tttgtccttt tcagtaatcc cgggcagctg taccgaaact
     2041 cctggtacat taaccccttt tttatttttc aatgtaccgg aattgataac aattgtatga
     2101 atcagacctt tttctagatc ttttccgaca acttccaact gaatcagacc gtcatccaaa
     2161 agaatgacag accctttgtc tacatcctcg atcagttttt catatgtaac tgaaaaaaca
     2221 ttatcgtcgc cttccacttc agtcatcgaa atatcgatat gatgaccagt gatcaattcg
     2281 acttgtccat ttttcatgga atgcgtccgg atttcagggc ctttcgtgtc aagcaggatt
     2341 ccgacgactt taccagtctt ctttgacgct tccctgatgg atttgattcg ggctttgtgc
     2401 tcttcgtgtg taccatgtga aaagttaagc cgtgcaacgt tcatacctgc ctcaattaat
     2461 tgttcaagta gctccggtga ttcgctggct ggtccgattg tgcaca
//
LOCUS       DASULI010000286         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_161145, whole
            genome shotgun sequence.
ACCESSION   DASULI010000286 DASULI010000000
VERSION     DASULI010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161145"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VFX34_06075"
     CDS             complement(<1..1101)
                     /locus_tag="VFX34_06075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5564507.1"
                     /translation="MDTKLAVFALGGINEIGKNMYVVQYEDDIVVIDCGSKFPDENLL
                     GVDLIIQDISYLKEHRENVRALIVTHGHEDHIGGIPYFLKQMNIPVYATRLTLGLIEL
                     KLKEHGLLRETKLVLIDGDSVVKLGAMSATFFRTNHSIPDCLGIAFETPEGTVVHTGD
                     FKFDLTPVNDDYADLHKMAEIGKKGVLLLLSESTNAERPGFSQSERVVGKHIADAFRE
                     ADQKIFISTFASNVHRVQQIIEAAHMTNRKIALLGRSMVNVVSVADELGYLDIPEDML
                     IEPQEIDRMNPENVVVICTGSQGEPMAALSRLSTNNFRQVSVYPGDTVIFASSPIPGN
                     EKSVSRIVDNLYLLGAKVIYGSGGSTGMHVSGH"
     gene            complement(1169..1240)
                     /locus_tag="VFX34_06080"
     tRNA            complement(1169..1240)
                     /locus_tag="VFX34_06080"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1206..1208),aa:Asn,seq:gtt)
     gene            complement(1345..1716)
                     /locus_tag="VFX34_06085"
     CDS             complement(1345..1716)
                     /locus_tag="VFX34_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HEX5564508.1"
                     /translation="MGKTLMKISVVYFMIGISFGLYMSFTHVFTLTSVHVHLNLVGWV
                     SLGLAGVFYHLYPCLEESGMAKAHFWLHNIGLPIMMISLATAILGGPEIFFLFATIGG
                     VMTVLGVLCFGFNVLFGLNKN"
     gene            complement(1807..3336)
                     /locus_tag="VFX34_06090"
     CDS             complement(1807..3336)
                     /locus_tag="VFX34_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX5564509.1"
                     /translation="MNMKDAGVKLRHALQELGPTFIKLGQIASSRRDLVPEEIAIELE
                     KLQDHVTSVPFDKIREIVEFELEAPLENIFSRFDENPLATASIGQVHVAYLPSGEKLA
                     VKVQRPDIRPNVETDLAILSDLARFLEDNTDWAKTYHLHEMIIEFSRSLQDELDYRVE
                     GRNCERIGKQFEEDPSIHIPKVHWDLSTDKVLTMDMVRGVKVNDLKKLDAGGYDRKLI
                     ARRIVDSMFHQILDEGFFHGDPHPGNIYILPQNVVSYLDFGMVGRLDNELKYHFASLL
                     IHLREGNTEDMIDTFSAMGILSDDTDMRAFTRDVDDLQTRYYDVALNEVSLGAIFIEL
                     FQVAYRHHIIIPSEISILGKAILTLEGLIAKLDPELSIMKAVEPFGRKLMRERYHPKH
                     LIENSWKDFVDNIEILTNLPKDLKAIATTIRKGKLQLDINVQQVQVFLRRLDRISNRL
                     SFSIILLSFSILMVGLIIGSAIAGQTNMLWQFPIIEAGSIIATLMFLFLIYTIIRSGR
                     M"
     gene            3800..>4038
                     /locus_tag="VFX34_06095"
     CDS             3800..>4038
                     /locus_tag="VFX34_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007129044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX5564510.1"
                     /translation="MDILLFITIILLASILQTGTGFGFSIVATPFLLLLFEARDAIQI
                     NLLLSLVISLAMYRKVKGDIDMGVMRRFIGGSFIG"
BASE COUNT         1069 a          998 c          924 g         1047 t
ORIGIN      
        1 atgtccggaa acatgcatgc ctgttgaacc gccagacccg taaatgactt ttgctccaag
       61 aaggtataga ttatcgacga tgcgcgatac gcttttttca ttccctggaa tcgggctgga
      121 ggcgaagatg accgtatctc caggatagac ggatacttgg cggaaattat tggtcgatag
      181 acgcgacagc gcagccatcg gttcgccttg gctgccggta cagatgacga cgacgttttc
      241 aggattcatc cggtcgattt cctgtggttc gatcagcata tcttcaggga tgtccaaata
      301 cccgagttcg tcagcgacag agacgacatt caccatgctt ctgccgagca gggcgatttt
      361 ccggttcgtc atgtgcgcag cttcaatgat ttgctggaca cgatggacat tcgaagcgaa
      421 cgttgagatg aaaatctttt ggtccgcttc ccggaacgcg tccgcgatat gcttgccgac
      481 aacccgttcg gattgcgaaa accctgggcg ttccgcattc gtgctttcgg ataataaaag
      541 aagaacaccc ttcttcccga tttcggccat tttatgaagg tccgcgtaat catcatttac
      601 cggcgtcaaa tcaaatttga aatcgcccgt atgcacgacc gttccttcag gtgtttcgaa
      661 ggcgatgcca aggcaatcgg gaatactatg attcgtcctg aagaaggttg cggacattgc
      721 tccaagtttg acaactgaat cgccatcgat caatacaagc ttggtctccc gtagcagtcc
      781 gtgctctttt aatttcaact caataagccc cagtgtcagt ctagttgcat acacggggat
      841 attcatttgt ttcaggaaat acggaatgcc gccgatatga tcctcatgtc catgcgtgac
      901 gatgagtgcc cggacatttt cccggtgttc cttcaagtag gagatgtcct ggatgatcaa
      961 atcgacgccc aataaattct cgtccggaaa tttcgaaccg caatcgatga cgacgatatc
     1021 gtcctcgtat tgaacgacgt acatattttt cccgatttca ttgatgccgc ccaaagcaaa
     1081 aaccgctaat tttgtgtcca catcgatgtc ctcccacact agataatgaa aaagggctgt
     1141 cccgtcatat cgacagagac agcctttact ccgaaagtag gattcgaacc tacggcctat
     1201 cggttaacag ccgattgctc caccgctgag ctatttcgga aggatattac taatcctata
     1261 ttgtgcaggt tgaaaagaat tattcatgtt tatttcaaaa aaatcacctc caataacaaa
     1321 tcggaggtgc aaatcaaacg ggtattaatt tttattcaaa ccgaaaagta cgttgaagcc
     1381 gaagcaaagg acacctagca cggtcatcac gccgcctatc gttgcgaaca ggaagaaaat
     1441 ttccggtcct ccgagaatag ccgttgcaag gctgatcatc atgattggca agccgatatt
     1501 atgaagccag aagtgcgcct tcgccatacc cgattcttcc aaacacggat agaggtgata
     1561 gaataccccc gccaatccaa gcgagaccca tccgaccaaa ttcaaatgga cgtgcactga
     1621 cgtcaaggta aacacatgtg taaaagacat gtataagccg aaagaaatgc cgatcatgaa
     1681 atatacgact gagatcttca tcaacgtttt ccccattttc cccacacctc cctccaacat
     1741 agaaatctat ctatactatt ctatgttgga caatcttcat tgaatgaggc tgcggagaag
     1801 aaaacttcac attcgcccgg accggatgat cgtgtagatg aggaatagga acatgagcgt
     1861 ggcaatgatg gaacctgctt caatgatcgg gaattgccaa agcatgttcg tctggcccgc
     1921 gatggcggag ccgatgatca agccgaccat gagaatgctg aaggaaagca ggatgatgct
     1981 gaaggaaagt cgattgctga tccggtcgag cctgcgcaaa aagacttgca cttgctggac
     2041 attgatgtcg agctgcaatt tcccttttcg aattgtcgtt gcaatggctt tcaaatcttt
     2101 cggcagattc gtcaggattt caatattatc aacgaagtct ttccacgaat tctcgattaa
     2161 gtgcttcgga tgatagcgct cccgcatcaa tttcctcccg aacggttcga ccgctttcat
     2221 gatgctcagc tcgggatcca gcttcgcgat caacccttct aatgtaagga tcgcctttcc
     2281 taaaatcgaa atctccgatg ggatgatgat atggtggcgg taggcgactt ggaacagttc
     2341 gatgaaaatg gccccaagac tcacttcatt caatgcaaca tcatagtacc tcgtttgaag
     2401 atcgtccacg tcccttgtaa acgcccgcat atccgtatcg tcagaaagga tgcccatcgc
     2461 ggagaacgtg tcgatcatgt cctccgtatt cccttcacgc aaatggatga ggagggaggc
     2521 gaaatggtat ttcagttcat tatcgagtct gccgaccatc ccgaagtcca aataggagac
     2581 gacattttga gggagaatgt aaatattccc cgggtgcgga tcgccgtgga aaaagccttc
     2641 gtcgaggatt tggtggaaca tcgaatcgac aatccgtctg gcgatcagct tccggtcata
     2701 cccgccggcg tccagctttt ttaaatcatt cactttgacc ccgcggacca tgtccattgt
     2761 aagcaccttg tcagtcgata aatcccaatg gactttcgga atatggatgc tcggatcctc
     2821 ctcgaactgc ttcccgatgc gctcgcagtt gcgcccttcc actcggtaat ccagttcatc
     2881 ttgcaaggac cttgaaaatt caatgatcat ctcatgaaga tgatatgttt ttgcccaatc
     2941 tgtattgtct tcaaggaagc gtgccaggtc gctcaatatc gccaggtccg tctccacgtt
     3001 aggccggatg tcgggacgct gcactttaac agccagtttt tccccgctag gcaagtaggc
     3061 gacatgaact tggccgattg aagcggtggc caatggattt tcatcaaatc tgctgaatat
     3121 gttttcaaga ggcgcctcga gttcgaactc tacaatttca cgaattttat cgaacggaac
     3181 agaagtgacg tgatcctgca gtttttcaag ctctatggcg atttcctctg gtacaagatc
     3241 ccgacgagag cttgcaatct gcccaagttt aatgaaggtc ggtcccaatt cctgcagtgc
     3301 atgacggagt ttgactcctg catctttcat attcatttca ttcccactat cgtttgacga
     3361 gccgcgatcg gttagcccta atcggaaaag aaagtggctg aacccattgc gtagaaagac
     3421 attcattatc tcctgaaacc gtttagtatg ccgaatgcgt attttgaaca aatccattcc
     3481 tccaaatcta tcgcagcagt ttatttttcc atataccctt ttcggggcag ggttagccat
     3541 gggactctgc tgaaaatgtg caggtggaaa gtcccgagat gagcgtaact agtcaaaagt
     3601 cgagcataac tcatgcaaac tcgcgcataa ctcagtccaa ttgcgccata actcatcgag
     3661 attgcgccaa aaccgactga aattcgcgca aaacactgaa tcgaaaatac gcggatgcac
     3721 aatcgaaggg gcagccgctc acaaactttg cgattcaatc cgtgttacac ttaatgaaaa
     3781 tcaaaagagg gtcattcata tggacattct tttattcatc acaatcatac tactggcctc
     3841 catcctacag acagggaccg gcttcggatt ctccattgta gcgacgccgt ttctcctgct
     3901 attattcgag gcgcgggacg ccattcaaat caatcttctc ttgtcattgg tcatttcatt
     3961 ggcgatgtac cggaaagtaa aaggggatat tgatatgggg gtcatgaggc ggttcatcgg
     4021 cgggagcttt atcgggtt
//
LOCUS       DASULI010000287         6497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1335113,
            whole genome shotgun sequence.
ACCESSION   DASULI010000287 DASULI010000000
VERSION     DASULI010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335113"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFX34_06100"
     CDS             complement(<1..701)
                     /locus_tag="VFX34_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564511.1"
                     /translation="MIVTAKQKRTIYILWLLVVPMTFYIALNYFPAKSYNYVNLVIYF
                     AILVATLMTSISLDKVSMTLEKWVIFSLFFQYGIVAEMIFMQIAIFILLYVQKSPTPK
                     AFRFAFNSINFAVVSLISGAIFYAVGGSLDKMTLGAFLLAGFIYASAYSLINSVIIYS
                     LFKWMKIDSAGMKKVVSWDYVIAILLLPFAITFSLLMREFGSEAFLLIGVPFILVLLV
                     TSNYMKSDNMNEILSS"
     gene            complement(720..2423)
                     /locus_tag="VFX34_06105"
     CDS             complement(720..2423)
                     /locus_tag="VFX34_06105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX5564512.1"
                     /translation="MSEKKLSTIILFIIWLLIVPPGIAYFLITQMPESINYAYLLLFI
                     IFGVLTAVFPIMMNGQPITLVMWATLPAFLMYGIAIEMIIMQLSVLAILFVKKSSQKK
                     SHRFFINSIIMFILSAVAAGAFFVVGGKTGSMDFWTLLIGVAVYRLVHTTTNLGLLNM
                     YFEAMGLKSPFTTKDNIFEYSTLILILPLALTFYLMLNMVGIASFLLLGFPFFMTITV
                     IRLYGRSEKINEYIKKAGQIGSELSVMTDEESVSTQFIKKTSELFRADYVFLFTNHDE
                     WLELEKWYEKKRFVDRPFQTVQLGEGIAGMVFEKKEPVIYSKRKDWEQYATNSAPEEL
                     ESVLCIPLNHNNETNGILLLGSNRKSAFGDHQLKILDLLGSYYIVALEKAGYVYEALQ
                     RSERCALTKLYNYRYLEEKLHFEMDRMGMGDIKDLSVVMLDIDHFKKVNDTYGHQSGN
                     DILYGFARILEKALPDNGTIGRYGGEEFVYILPDFSKEEAVDFAERLRLLIRQHAFTI
                     IPDLGEDKYPVKVSITSSVGVASAPTDTDEAMNLLRNADRALYIGAKQAGRDRVAEYV
                     R"
     gene            complement(2508..3716)
                     /locus_tag="VFX34_06110"
     CDS             complement(2508..3716)
                     /locus_tag="VFX34_06110"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX5564513.1"
                     /translation="MIPKFDEYKERFDIESDDEIKPGLDRIRAALKKVGNPEKGLCVI
                     HVAGTNGKGSTIAFMESILKEHGFSTGVFSSPALVDVHDQIRMNGVPIRPEEMDRTFM
                     VMKEAGLSGTLTDFELLTVAAFTTFERLAPDYVLLECGMGGRLDSTNVVMPLVSVIPS
                     IALDHVGFLGGRIEEIAEHKAGIIKEGAPVVVGPMPEDAMNVIRMKVRQMNSSLAVYG
                     DDFTLSEEEFIGGSIRVQIGGRRMKGPHQAVNAAIAIEALLQANILMNEESIAKGVAA
                     ASLPYRFQEIAEGVFFDGAHNPAAAKMLAETIKSEFPGEKVDFVIGMLKTKDIKKTLN
                     ELIPVAASFTFVTFPHPQAATAEELLSSCQYNRKRVTKIEGDTIILRKGNDRKKIVAG
                     SLYLISSLLN"
     gene            3779..4645
                     /locus_tag="VFX34_06115"
     CDS             3779..4645
                     /locus_tag="VFX34_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HEX5564514.1"
                     /translation="MKKCWVIYNGSLTSDKFRDQAELVKDAAERAGVSATLMKNYEVL
                     MNLNEKLENRPDFVVFLDKDILLATFLKNAGIPVFNDPEVIETCDNKAKQYIQLAKNH
                     IAMPETIIAPKVYPKFTIEGTGYYERILEKLGLPMIIKEGHGSFGMKVYLIETEEEFF
                     EKVDELRGVDYVFQQFIATSRGRDIRVNIVGGEVVAAMYRSSETDFRANITNGGVASV
                     IELTEEQKDLAIRAADAVGAEFAGVDLLFGENEEPIVCEVNAAAHIRNIYNVTGINVA
                     DKMISYIVEKLG"
     gene            4642..5439
                     /locus_tag="VFX34_06120"
     CDS             4642..5439
                     /locus_tag="VFX34_06120"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5564515.1"
                     /translation="MKGHVYYSSAEAERNRGFIDDLMKHASRMGISLSLLVDMEEPDV
                     NADFIFFRDRNPVLSEKWESAGFRMINRSEVNKIANDKLRTFELSTLLGVSAVPTTKA
                     KTVLDLPSFPVVLKTVDGHGGNEVFLCHTADEAEAFFADFAGRKVIVQPYIESDATDV
                     RVFMLGGEVLGAVKRTGSDAFKSNYTLGGSVEKYALSAWQEKEVSKIAKALKSDYIGI
                     DFLLLPDGGWMLNEIEDPVGARSLYVSHDFSVAEKVVEYVKMKLESL"
     gene            complement(5510..>6497)
                     /locus_tag="VFX34_06125"
     CDS             complement(5510..>6497)
                     /locus_tag="VFX34_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010288150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5564516.1"
                     /translation="TGSSPGQDLRYSTEKVEAIWNFANKIWNASRFALMNMDGMTYDE
                     IDLSGEKSVADAWILTRLNETIEQVTKLAEKYEFGEVGRALYNFIWDDFCDWYIEMAK
                     LPLYGEDEAAKKTTRSVLAYVLDNTMRLLHPLMPFITEEIWQNLPHEGESITVAAWPV
                     ADPALTDKAKASDMKLLVDLIHAVRNIRAEVNTPMSKKVPLYIAAKDDAAVSVLEANR
                     SYIERFCNPETLEIGVGISAPGKSMSAVITGAELFLPLEGLLNIDEEIERLKKELAKW
                     EGEVKRVQGKLSNERFISKAPEAVVAEERAKEKDYLEKKAAVERRIEELKEI"
BASE COUNT         1804 a         1490 c         1431 g         1772 t
ORIGIN      
        1 gaagacaata tttcgttcat gttgtccgat ttcatataat tactcgtcac tagcaataca
       61 aggatgaacg gaacgcctat caataaaaac gcttcgcttc cgaattctcg cataagtagg
      121 ctgaatgtga tcgcaaaagg aagaaggagg atcgctatga cataatccca tgaaaccact
      181 ttcttcattc ctgccgagtc gatcttcatc catttgaata atgagtagat aatgacgctg
      241 ttaatcaatg aataagctga cgcataaatg aagccggcaa gcaggaatgc acctaatgtc
      301 attttatcta acgagccacc gacggcataa aaaattgcgc cgctgattaa tgaaacgaca
      361 gcaaagttaa tcgaattgaa tgcaaaccga aaagcttttg gcgtaggtga tttctgaacg
      421 taaagaagga tgaaaatggc gatttgcatg aagatcattt ccgctacaat tccatactgg
      481 aagaaaaggg agaaaatcac ccacttttcc aatgtcatgc ttactttgtc caatgaaatc
      541 gaagtcatca aagttgcaac gaggattgca aaatagatga ccaggttcac ataattataa
      601 gatttcgcag gaaagtagtt gagggctatg tagaacgtca taggaacaac aagtagccac
      661 agtatataga tcgttctttt ttgtttggcc gtcactatca attcctatcc cctcctttgt
      721 catcttacgt attctgcaac ccggtctctt cccgcttgct ttgcaccgat atataaggct
      781 cggtccgcgt ttcgcaataa gttcatcgct tcatccgtgt cggtcggtgc gcttgcaacg
      841 ccgacactgg acgtgatgga cactttcacc ggatatttat cctcacctaa atcgggtatg
      901 attgtaaatg catgctgcct aatcaacaat cgcagccgtt ccgcaaaatc gaccgcttcc
      961 tctttggaaa agtccggcaa tatatacaca aattcttcgc ctccataccg cccgatcgtc
     1021 ccattgtccg gcaatgcctt ctccaaaatc cttgcaaatc cgtacaatat atcgtttcca
     1081 ctttgatgtc cgtacgtatc gttcactttt ttgaaatgat cgatatcaag catgacgaca
     1141 gatagatctt tgatatcgcc catgcccata cggtccattt caaagtggag cttttcttcc
     1201 aaatagcgat aattatatag ctttgtcaat gcgcaacgtt cactccgctg caacgcttcg
     1261 taaacatatc ccgccttttc gagtgccaca atataatacg atccgagcaa gtcaagaatc
     1321 ttcagctgat gatccccgaa tgcggatttc cgattggaac ccagaaggag gatgccgttc
     1381 gtttcgttgt tatgattcaa agggatgcac agcacacttt ccaattcctc cggcgcggaa
     1441 tttgtggcat attgctccca gtctttccgc tttgaataga tgaccggttc cttcttctca
     1501 aaaaccatcc ctgcaatccc ttctccgagt tggaccgttt ggaaaggccg atcgacaaaa
     1561 cgctttttct cataccattt ttcaagctca agccattcat catgatttgt aaaaagaaat
     1621 acgtaatcgg ctctgaataa ctcacttgtt ttcttgatga attgagtcga aacggattct
     1681 tcatccgtca tgacagagag ctcacttcca atttgccctg ctttctttat atactcattg
     1741 atcttttcag aacggccata caggcgaata accgttattg tcataaaaaa gggaaagcct
     1801 aaaagtaaaa aagaagcaat gccgaccata ttaagcatca aatagaaagt cagagccaag
     1861 ggtaagataa gaatcagtgt cgaatattca aaaatattat cttttgtcgt aaacgggctt
     1921 ttcaatccca ttgcttcaaa atacatattt aatagaccga gattcgttgt tgtatgtact
     1981 agccgatata ctgcgactcc aattagcaga gtccaaaaat ccatcgaccc tgttttaccg
     2041 cctactacaa aaaacgcgcc agctgcaaca gccgataaaa tgaacatgat gatggaattg
     2101 atgaaaaaac ggtgggattt cttttggctg ctctttttca caaaaagaat ggccaacacg
     2161 gacaactgca taataatcat ttcaatagca attccataca tgaggaaagc aggtaatgtc
     2221 gcccacatga ccaaagtaat tggctgtcca ttcatcataa tcggaaaaac tgctgtcagc
     2281 acaccgaaaa tgatgaagag cagcaaatag gcataattta tgctttcagg catctgagtt
     2341 ataaggaaat aagcgattcc cggtggaacg atgagcaacc aaataataaa taggatgatt
     2401 gtggacaatt tcttttcaga caccgctcat gcacccctta acaagtataa aaatcagtga
     2461 aatgataggg tgtcgtccaa ttgacgatga tcaaatcctg tagcttatta gttcaaaaga
     2521 ctactgatca gataaagaga ccctgcgact attttcttcc tatcatttcc ctttcttagt
     2581 attatagtat caccttcgat ttttgtcacc ctttttctat tatactgaca actggatagt
     2641 aattcctctg cagttgcggc ttgcgggtgt ggaaaagtga cgaaagtgaa ggaagctgcg
     2701 acaggaatca gctcattgag tgttttctta atgtcttttg tttttagcat gccaatgaca
     2761 aaatcgactt tttcaccggg aaattccgat ttgatcgtct ctgcaagcat tttggcggca
     2821 gcggggttat gcgccccatc gaaaaagacc ccttccgcaa tctcctggaa tcgataaggc
     2881 aatgaagcgg cagccacgcc ttttgcaatc gattcctcat tcatcaatat attagcctgt
     2941 agcaacgcct cgattgcaat tgccgcattt acagcttgat gaggtccttt catcctgcgt
     3001 cccccgattt gcacccggat ggatcccccg ataaattcct cttcactcaa tgtgaagtca
     3061 tctccataaa ctgctaagct gctgttcatc tgacgcactt tcatccgaat gacattcatt
     3121 gcatcttcgg gcattggacc gacgacaaca ggtgccccct ctttaatgat ccccgcctta
     3181 tgttccgcaa tctcttcaat tctccctccg agaaacccga catggtccaa cgcaatcgat
     3241 gggataacag agactagcgg cataacgaca ttcgtactgt ccaacctgcc gcccatgccg
     3301 cattccagca ggacataatc cggagccaac ctttcaaaag tggtaaaagc tgcgaccgtc
     3361 agcaattcga agtccgtcaa agtgccgcta agaccagctt ctttcattac cataaacgtc
     3421 ctgtccatct cttcaggacg gatcggcacg ccgttcatgc ggatttggtc atgtacatct
     3481 accaatgccg gtgatgaaaa gacgcctgtc gaaaatccat gctctttcaa aatggattcc
     3541 atgaaggcga ttgtggaacc ttttccgttc gttcctgcca catggatgac acacaggccc
     3601 ttttccgggt ttccgacctt cttaagagca gcacggatcc tatcgagtcc cggctttatt
     3661 tcatcatcac tttctatatc aaaacgctct ttatattcat caaattttgg aatcaaaaca
     3721 ctcgcccctt atggtaaaat cagcataggt taagtatatc acggccggag ggaacgatat
     3781 gaaaaaatgt tgggtcatct ataatggaag tttaacgagc gataagttcc gcgatcaggc
     3841 ggaacttgtg aaggatgcgg cggaacgggc gggagtttcc gcgactctca tgaaaaatta
     3901 tgaagtgctt atgaatttga atgaaaagct ggaaaaccgc cccgattttg tcgtcttcct
     3961 ggacaaggat atcctgctgg cgacattttt gaaaaatgct ggcatccctg ttttcaatga
     4021 ccccgaagtg attgagacgt gtgataataa ggcaaaacaa tatatccaac tggcaaagaa
     4081 tcatatcgcg atgccggaaa cgatcatcgc gccgaaagtg tatccaaaat ttacgattga
     4141 aggaaccggc tattatgaaa ggattcttga aaaactgggc ttgccgatga ttatcaagga
     4201 aggacatgga tcattcggca tgaaagtata tttgattgaa acggaagagg agttctttga
     4261 gaaggtggat gagctgcgtg gggtcgatta tgtgtttcag caattcatcg caacaagcag
     4321 gggacgcgat atccgcgtca atatcgttgg aggtgaagtc gttgcggcaa tgtatcgttc
     4381 ttcagagacg gatttccggg cgaatattac gaatggagga gttgcgagcg ttattgaact
     4441 gaccgaggag cagaaggatt tggcaatccg cgcagctgat gcggtcggtg cagaatttgc
     4501 aggcgtcgat ctattattcg gggaaaatga agagccgatt gtctgtgaag tgaatgccgc
     4561 ggcgcatatc cggaatattt ataacgtgac gggcattaac gtagcggata agatgattag
     4621 ctacatagtg gagaaactgg gatgaaggga catgtttatt attcctcggc ggaagcggag
     4681 cgaaatcggg gatttatcga tgatctgatg aagcacgcgt cgaggatggg gatatcattg
     4741 agtttgctcg tggatatgga agaaccggat gtcaatgcag atttcatttt cttccgcgac
     4801 cggaacccag tgctatcgga aaaatgggag tctgctggat tcagaatgat caatcggtct
     4861 gaagtgaaca agatagcaaa tgataaattg aggacctttg aactgtccac attgttaggg
     4921 gtttcagcgg tccccacgac aaaagcaaag acagtgcttg acctcccctc tttccctgtc
     4981 gtcttgaaaa cggttgatgg acatggcggc aatgaagttt tcctttgcca tacggcagac
     5041 gaagcggagg catttttcgc tgattttgca ggcagaaagg taattgtcca accatatatc
     5101 gaatctgatg caacggatgt acgtgtattc atgcttggag gggaagtcct gggagctgtt
     5161 aaaaggactg gcagtgacgc attcaagtcg aattatacac taggcggttc agtcgagaag
     5221 tatgcacttt cagcttggca ggaaaaagag gtcagcaaaa tagcgaaggc gttgaaaagc
     5281 gattatatag ggattgattt tcttttattg ccggacggcg gttggatgct aaatgaaatt
     5341 gaagacccag tcggcgccag atcgctatat gtgtcgcatg atttttcagt tgcggagaaa
     5401 gtggtggagt atgtgaagat gaaattggag agtttatgat cacttgcgcg agaaaacaaa
     5461 aaaagagttg gtccccctat tatggaggtc gaccaactct ttttcgtcat taaatctctt
     5521 tcaattcttc aatccggcgt tcgactgccg ctttcttctc caagtaatcc ttctccttcg
     5581 cgcgctcttc cgcaacgact gcttcaggcg ctttcgagat gaaccgctca ttggaaagct
     5641 tgccttggac gcgtttcact tcgccttccc attttgccag ttccttcttc aatcgctcga
     5701 tctcttcgtc gatattgagc agcccttcaa gcggaaggaa tagttctgct cctgtaatga
     5761 cagccgacat cgatttgcct ggcgctgaaa tgccgacacc gatttcaagt gtttccggat
     5821 tacagaagcg ttcgatatag cttcggttcg cttccaatac ggaaacagcc gcgtcatctt
     5881 tcgctgcgat ataaagcgga actttcttgc tcatcggcgt gttcacttct gcccggatgt
     5941 tgcggactgc gtggatgagg tcgacaagca acttcatatc cgaagctttt gccttatccg
     6001 tcaatgcagg atcagccacc ggccaagctg ccactgtgat cgattcccct tcatgcggaa
     6061 ggttctgcca gatctcctcc gttatgaacg gcattaacgg gtgtaagagg cgcatcgtat
     6121 tgtcgaggac gtaagcaagt actgaacgag tcgttttctt cgccgcttca tcttctccgt
     6181 acaatggcaa tttcgccatt tcgatgtacc agtcgcagaa atcgtcccag atgaaattgt
     6241 aaagcgcacg gccgacttct ccgaattcgt atttctcggc aagcttcgtc acttgttcaa
     6301 tcgtttcatt caaccgtgta agaatccaag catccgccac ggatttttca ccggagagat
     6361 cgatttcatc gtacgtcatg ccatccatat tcatcaaagc gaaacgggat gcattccaga
     6421 ttttattcgc aaagttccag atcgcttcta ctttttccgt tgaatagcgg agatcctgac
     6481 ctggcgaaga accggtc
//
LOCUS       DASULI010000288         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_310553, whole
            genome shotgun sequence.
ACCESSION   DASULI010000288 DASULI010000000
VERSION     DASULI010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310553"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFX34_06130"
     CDS             complement(<1..491)
                     /locus_tag="VFX34_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX5564517.1"
                     /translation="MKENYLGVNVSPLTYEEIIKEIQSRIEQKKQSTIIAVNPEKVMA
                     AQKNEELKQLINNSTFQIADGVGILLASKLKKGRIRSRVTGVDMMARLLKFAADEKKP
                     IYLYGAKQEVVELATNNMLRDYPGLQIAGTTNGYEKDEEALVQRINESGAELLFVALG
                     SPK"
     gene            702..1826
                     /gene="csaB"
                     /locus_tag="VFX34_06135"
     CDS             702..1826
                     /gene="csaB"
                     /locus_tag="VFX34_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HEX5564518.1"
                     /translation="MKIVLSGFYGLGNTGDEAILKAIVDNLRAELDNPDITVFSLSPE
                     KTAKEYGVKSVYRAWRRENGKKVKALREADLLISGGGGLLQDTYPTKFLFGPLPYYLL
                     IVFLAKLCGTKVMFFSQGIGPVTSKWGKFLMRIFANMADFITVRDERSKEELHALGVT
                     RPETVVTADIVFAFKPTEDHVAYASLGLKGNERLVAVAPRPWFDHEDEYIEKLAWVLD
                     ELIDQRGVLPVFVPMEPPYDTNVSKKVIAKMKNGSKTKLLGETFTPNEFYNFISRTEL
                     TIALRLHALIFAALSNVPHVGLSYDQKVESFLKRSGMWKHSFPLGDFTKEDLLEQSLY
                     ALDHSEEVKQMMEPNVDVLRAEALRNIDLLRERFLDGGKG"
     gene            1827..>2423
                     /locus_tag="VFX34_06140"
     CDS             1827..>2423
                     /locus_tag="VFX34_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5564519.1"
                     /translation="MKILLATAYDYPHLGGLSVHLTTLKAGLESRGHDVDIVSFSDVP
                     KWKRDGVVRGPAFVLNKMKKGSGYVWTLKRRKDELESLIKEKVSGGGYDIINAQDAYT
                     TLAALESDVPVVSTVHGYLTFEGISKGTVMENSAEADELMDAERRSYKSTREVITVDT
                     RIKDYIEKEAGVVGNMIKNFIDVESFKPEVERRDEFREK"
BASE COUNT          553 a          500 c          678 g          692 t
ORIGIN      
        1 tgtttcgggc ttccgagggc gacaaataga agctctgccc cgctttcatt aatccgctgg
       61 acgagcgctt cttcatcctt ttcatagccg tttgtcgtcc cggcgatttg aaggccaggg
      121 tagtcgcgga gcatgttgtt cgttgccagc tcgactacct cttgcttggc gccgtacaaa
      181 tagatcggct tcttttcatc agccgcgaat ttgaggagcc ttgccatcat gtcgacgcct
      241 gtgacacgtg aacggatacg cccttttttc agctttgatg cgagtaggat tccgactccg
      301 tccgcaattt ggaatgtgga gttgttgatg agttgtttca actcctcgtt tttctgcgcc
      361 gccatcactt tttccggatt gacggcgatg atggtcgatt gctttttttg ttcgattctt
      421 gattggatct ctttgatgat ttcttcatat gtgagcggcg aaacattgac gcctaaataa
      481 ttttccttca tgtacaaacc ctctatccct tcgctttcaa ctgtatcagt atagcagatt
      541 tccatggcgg ttgcataggt gctatgaaca gtgttaaaat cttgtaagaa tgctgtaatc
      601 aatagtggcc attcgtatac gtatacacgt tgctttcacg gtgcaagtga aggcttttat
      661 tatgtagtgg gggctttttt cattttacag gaggttattt tatgaagatt gtgctttcgg
      721 gcttttatgg ccttgggaat acaggcgacg aagcgatttt gaaggcgatt gtcgataatt
      781 tgcgtgcgga gctggataat ccggatatta cggttttttc gctttccccg gaaaagaccg
      841 cgaaagaata tggggtcaag tccgtgtatc gtgcttggcg tcgtgagaac ggcaaaaagg
      901 tgaaggcgct gcgtgaagct gatctgctca tttcgggggg cggcggcctg ttgcaggata
      961 catatccgac gaaattttta ttcggaccgc ttccctatta tttattgatc gtatttttgg
     1021 ctaaactttg cggtacgaag gtgatgttct tttcacaagg gatcggaccc gttacaagca
     1081 agtggggcaa gttccttatg cgtatcttcg ccaacatggc cgattttata actgttcgtg
     1141 acgaacgttc gaaagaggag ctgcatgcgc tcggggtgac acgtccggaa acggtcgtta
     1201 cggcggatat cgtgtttgca ttcaaaccga cggaggatca tgtcgcttat gcatcgctcg
     1261 ggctgaaggg caatgagcgt ttggtggctg ttgcgccgcg tccttggttc gatcatgagg
     1321 atgaatatat cgaaaagctt gcgtgggtgt tggacgagct gattgaccag cgcggcgtgc
     1381 tgcctgtttt cgtcccgatg gaacctcctt atgacacgaa cgtctctaag aaagtgatcg
     1441 cgaaaatgaa gaacggctcg aaaacgaaat tattgggtga aactttcacg ccgaatgagt
     1501 tttataattt cataagccgt acggaattga cgatcgcgtt aaggctgcat gcacttattt
     1561 tcgctgcatt gtcgaatgtt ccgcatgtcg ggctcagcta tgatcagaaa gtggaaagct
     1621 tcctgaaacg ttcaggcatg tggaagcatt cattcccact cggcgacttc acgaaggaag
     1681 atctgctgga gcagtctttg tatgccttgg atcattccga ggaagtgaaa cagatgatgg
     1741 agccgaatgt ggatgtgtta cgcgctgaag cgttgcggaa catcgattta ttgcgggagc
     1801 gttttttaga tggaggtaaa ggataaatga agattttact tgcaacagct tatgattatc
     1861 ctcatttggg cggcttgtcc gtccatttga cgacgttgaa ggcggggctc gaatcccgtg
     1921 ggcatgacgt ggatattgtt tcattctccg atgtgccgaa atggaagcgg gatggcgttg
     1981 tccgcggacc ggctttcgtt ttgaataaaa tgaagaaagg ttcgggctat gtctggacgt
     2041 tgaaacgacg gaaggatgag cttgagtcgt tgatcaagga gaaggtttct ggcggcggat
     2101 atgacatcat caatgcgcag gatgcttata cgacgcttgc cgcgcttgaa tcggacgtcc
     2161 ctgtcgtcag tacggtccat ggctatttga cgttcgaggg gatttcaaaa gggacggtca
     2221 tggaaaactc cgctgaagcg gatgagctga tggacgcgga gcgcagatcc tataagtcga
     2281 cacgtgaagt cattacggtg gatacgcgga tcaaggatta tattgaaaaa gaggccggcg
     2341 tcgtcgggaa tatgatcaag aacttcatcg acgttgagtc gttcaagcct gaggttgagc
     2401 gccgggatga gttccgtgaa aaa
//
LOCUS       DASULI010000289         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1548399,
            whole genome shotgun sequence.
ACCESSION   DASULI010000289 DASULI010000000
VERSION     DASULI010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548399"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFX34_06145"
     CDS             complement(<1..459)
                     /locus_tag="VFX34_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5564520.1"
                     /translation="MFGLGSRDVGIDLGTANTLVFIKGKGIVLREPSVVAKNTVTNEI
                     VAVGSAAKNMIGRTPGSIVATRPMKDGVIADFEITTAMIEHYMKEATKAAGSAWKKPN
                     VMVCVPFGITSVEQRAVIDAARQAGAKDAFTIEEPFAAAIGANLPVWEPTG"
     gene            complement(699..1376)
                     /gene="radC"
                     /locus_tag="VFX34_06150"
     CDS             complement(699..1376)
                     /gene="radC"
                     /locus_tag="VFX34_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX5564521.1"
                     /translation="MMIRDVHIADRPRERLIRQGAGSLSNQELIAILLRTGTKEESVL
                     MLANRILKSVDKIQDLKDVNVEEMMAVKGIGQAKAVQLLAAVEIGKRISRKHSEGRYV
                     IKSPEDAAAYLMTDMSSLVQEHFVVLFLNVKNEVLHKQTIFIGSLNSSIVHPREIFRE
                     AVKRSAASIICAHNHPSGNPSPSPEDIEVTRRLVEAGSIIGIELLDHVIIGDHRFISL
                     KEKGYMS"
     gene            complement(1405..2004)
                     /locus_tag="VFX34_06155"
     CDS             complement(1405..2004)
                     /locus_tag="VFX34_06155"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010287697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5564522.1"
                     /translation="MEFFTKGPIILASGSPRRQELLNNIGIPFDTMPSNVEEVVEFVE
                     GDFADYVQKLAVKKGAAVSEMKPDAFIIAADTIVVFDGEVYPKPSSKEQAESFLRKFS
                     GKTHSVFTGVSILGKGISNVFSVETKVTFKELDDQLIRAYVDSGDPMDKAGGYGIQTA
                     GSLLVDKIEGDYLNVVGLPLSKLVETLREEGIVLLKEAE"
BASE COUNT          529 a          569 c          432 g          604 t
ORIGIN      
        1 ccccgtaggt tcccaaactg gtaaattagc accgattgca gcagcaaaag gctcttcaat
       61 cgtaaacgca tcttttgccc ctgcttgtct agccgcgtca atgaccgcac gctgttcaac
      121 ggaagtgatg ccgaaaggta cacataccat gacgttcggt ttcttccatg cgcttcctgc
      181 agcttttgtc gcttctttca tataatgctc gatcattgct gtcgtaattt cgaaatccgc
      241 gatgacgcca tccttcatcg ggcgcgttgc cacgatggag cctggtgtac gtccaatcat
      301 atttttagcc gctgaaccga ctgccacgat ttcatttgtc actgtgtttt tcgctacgac
      361 ggacggttcc cgaagaacaa tcccttttcc tttaatgaaa accaatgtat ttgctgtgcc
      421 gaggtcaatc ccgacatctc ttgatcctaa tccaaacaaa tctgttcttc ctttctattc
      481 gaccgaataa tatggtcttc ttgaaatcat acaccacatt ataacggatt ggagaacaaa
      541 ttcacagtgt caatagcaat agactggatt ttgatgctat cgccttcctt ctccaagcag
      601 ctcaccgagt cacccgtatt cccttcacac tgtagggtca ggcgcctctt ccctatcttc
      661 attgcaaata aaccgattac cagtttagga gtaatttgct aactcatata tcctttttct
      721 ttaagactta tgaatcgatg atcaccgata atgacatgat cgaggagttc gattccgatg
      781 atggatcctg cttcgacaag tcgccttgtc acctcgatgt cctccgggga tggcgaggga
      841 ttcccggaag gatggttatg tgcgcaaata atagaagcag ctgaacgttt caccgcttca
      901 cggaaaatct cgcgtggatg cacgatggaa gaattcagac tgccgatgaa aatcgtctgt
      961 ttgtgcagca cttcattttt cacgttcaga aagaggacga cgaaatgctc ctgaacgagg
     1021 gatgacatat cggtcattaa ataagccgca gcgtcttccg gcgattttat gacataacgg
     1081 ccttccgaat gcttccggga tatccgcttg ccgatttcga ctgcggcaag cagttggacg
     1141 gccttcgctt gtccgatccc tttgacagcc atcatttctt ccacgtttac atcttttaga
     1201 tcctgaattt tatcgactga ctttaagatc ctgtttgcaa gcatcagaac tgactcttcc
     1261 ttcgtgcccg tccgcaatag gatggcaatg agttcctgat tcgacaagct ccccgccccc
     1321 tggcggataa gtctctcacg aggtctatcc gcgatatgta catcccggat catcatctgt
     1381 ttcttcacat caactgtcaa agaatcattc agcctccttc aacaagacga tgccttcttc
     1441 acgcaatgtc tccacaagct tcgataaagg caatccaaca acattcaaat agtccccttc
     1501 aattttatcg acaaggagcg aacccgcggt ttggatgcca tacccgccgg ccttgtccat
     1561 cggatcgccg gaatcgacat atgcacggat cagctggtcg tcaagctcct tgaaagtcac
     1621 tttcgtctca actgaaaaga cgttcgaaat gcctttgccg agtatgctga cacccgtgaa
     1681 tactgaatgg gttttgccgg aaaactttct caggaagctt tctgcctgtt ctttcgatga
     1741 cggtttcgga taaacctcac cgtcaaagac tacaatcgtg tcggcagcaa taataaaagc
     1801 atccggtttc atctccgaga ctgctgcccc ctttttgact gcgagtttct ggacatagtc
     1861 cgcaaagtcc ccctccacaa actcaaccac ttcttcgaca ttgctcggca tcgtgtcgaa
     1921 cggaatgcca atattattta gcagctcctg ccttctaggg gagcctgatg ccaggataat
     1981 cggtcctttt gtaaagaatt ccatttaagc aacctccttt ttctttatca tactgtagaa
     2041 ggaggggatt ggaaattttc acaaatccgc aaaaacgcga ttttaccccg atttcccgtg
     2101 ttaaaaatta attttcacac agccgtcctt atgc
//
LOCUS       DASULI010000290         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1719520,
            whole genome shotgun sequence.
ACCESSION   DASULI010000290 DASULI010000000
VERSION     DASULI010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719520"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="gpmI"
                     /locus_tag="VFX34_06160"
     CDS             <1..1041
                     /gene="gpmI"
                     /locus_tag="VFX34_06160"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5564523.1"
                     /translation="YIEQTEKVMEGLEVGKIATVSGRFYAMDRDKRWDRVERAYKAIV
                     DGTGEQFKNATEGILSSYDKGVHDEFVEPFIIAEEGKPVATVKDGDAVVFFNFRPDRA
                     IQLSKAFTDPAFDGFPTGPETFGNLKFVTFTQYSDEIRADIVFSNENLGNTLGEVISK
                     NGLRQLRIAETEKYPHVTFFMSGGKEDEFEGEKRILIASPKVATYDLKPEMSAVEVTE
                     ALLKEIEDETQDAIILNFANPDMVGHSGMLEPTVKAIETVDACLGRIIDALMLKGGTA
                     IITADHGNSDEVVTMEGKPMTAHTTNPVPVIITKPDIELREGGILSDLAPTMLKLLEI
                     EQPEEMSGTPLF"
     gene            1059..>2283
                     /gene="eno"
                     /locus_tag="VFX34_06165"
     CDS             1059..>2283
                     /gene="eno"
                     /locus_tag="VFX34_06165"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5564524.1"
                     /translation="MPIITHIQAREVLDSRGNPTVEVEVFTESGAFGRAIVPSGASTG
                     EYEAVELRDGDASRYLGKGVLKAVDHVNEIIADELVDNYSVLDQVVVDNALIELDGTK
                     NKGKLGANAILGVSMAVAHAAADYLDLPLYQYLGGVNAKQLPVPMMNILNGGEHADNN
                     VDIQEIMVMPVGAESFRHGLRMGTEIFHSLKDVLKSKGLNTAVGDEGGFAPNLASNEE
                     ALSTIMEAIENAGYKAGEEVLLAMDVASSEFYDSEAKTYRLAGEGITKTSEEMVAWYE
                     ELCSKYPIVSIEDGLDENDWAGHKLLTERLGAKVQLVGDDLFVTNTEKLERGIEEGVG
                     NSILIKVNQIGTLTETFDAIEMAKRAGYTAVISHRSGESEDTTIADIAVATNAGQIKT
                     GAPSRTDRVAKYNQLL"
BASE COUNT          630 a          545 c          647 g          461 t
ORIGIN      
        1 tatatcgagc agaccgaaaa ggtgatggaa ggacttgaag tcggtaaaat cgccaccgtg
       61 tccggacgtt tctacgcaat ggaccgggat aaaagatggg atcgcgttga gcgggcatac
      121 aaagcgatcg tcgacggcac gggtgagcaa ttcaaaaatg ccactgaggg catcctgtct
      181 tcgtatgata aaggcgtcca tgatgagttc gtggaaccgt tcatcattgc ggaggaaggc
      241 aagcccgtcg caacggtgaa agacggagac gcagtcgtct tcttcaattt ccgtccggac
      301 cgcgcgattc aattgtcgaa ggcgtttaca gatccggcgt tcgacggctt ccctaccgga
      361 ccggaaacgt tcggaaactt gaagttcgtc acattcacgc aatatagtga tgaaatccgg
      421 gcggacatcg tgttttcaaa tgaaaacctg gggaacacac tcggtgaagt gatttcgaaa
      481 aacgggctcc ggcagctgcg gattgccgaa acggagaagt acccgcatgt gaccttcttc
      541 atgagcggcg gcaaggaaga cgaattcgaa ggcgaaaaaa ggattctcat cgcgagtccg
      601 aaagtcgcga catatgactt gaagccggaa atgagtgccg ttgaagtgac cgaagcgctg
      661 ttgaaagaaa tcgaagacga aacgcaggac gcaatcatcc tcaatttcgc gaaccccgac
      721 atggtcggtc atagtggtat gctggagccg acggtcaagg cgattgaaac ggtcgatgcg
      781 tgcctcggca ggatcatcga cgccctcatg ctgaaaggcg ggacggcgat catcacggcg
      841 gaccacggga attccgacga ggtcgtgacg atggaaggaa agccgatgac tgcgcatacg
      901 acgaatcctg tgccggtcat cattacaaag cctgatatcg aattgcggga aggcggcatc
      961 ctatcggatc ttgccccaac gatgttgaag cttctagaaa tcgaacagcc ggaagaaatg
     1021 tccggaacac cattattcta ataaagggag agattgaaat gccaatcatt actcatatcc
     1081 aagctagaga agttcttgat tcacgcggta acccgactgt tgaagtcgaa gtgttcacag
     1141 aaagcggagc attcggccgc gccatcgttc cttcaggtgc ttccacaggt gaatatgagg
     1201 cggtcgaact gcgcgacggc gacgcatccc gctaccttgg aaaaggagta ttgaaagcgg
     1261 tcgatcacgt caatgaaatc attgcggatg aactcgtgga caattattcg gttctggatc
     1321 aagtcgttgt ggacaacgcg ctgattgaac tggatggcac gaaaaacaaa ggcaagctcg
     1381 gcgcaaacgc gatccttggc gtttcaatgg cagtcgcaca tgctgcagcg gattatttgg
     1441 accttccgct ttaccaatac cttggcggcg tcaacgcgaa gcaattgccg gttccgatga
     1501 tgaacatctt gaacggcgga gagcacgcgg ataataacgt cgatatccag gagatcatgg
     1561 tcatgccggt aggcgcggaa tcattccgcc acggccttcg catggggact gaaatcttcc
     1621 atagcctgaa agacgtcttg aaatcgaaag gcctaaacac tgcggtcggc gatgaaggcg
     1681 ggttcgcacc gaacttggca tcgaacgaag aagcgctttc gacaatcatg gaagcgatcg
     1741 aaaacgctgg ctacaaagca ggcgaagaag tattgctcgc gatggatgtc gcttcttccg
     1801 agttttacga tagcgaagcg aagacgtacc gtttggcggg tgaaggaatt acgaagacat
     1861 ccgaggaaat ggtcgcttgg tatgaagagc tttgctcgaa atatccgatc gtctccatcg
     1921 aagacggcct tgacgaaaac gactgggcag ggcataagct gttgacggag cgcctcggag
     1981 ctaaagtcca attggtcggg gacgatctgt ttgtcacgaa cactgagaaa ttggagcgcg
     2041 gcattgaaga aggcgtcggc aactcgatcc ttatcaaagt gaaccagatc ggtacattga
     2101 ctgaaacatt cgatgctatc gaaatggcga aacgcgctgg ctatacagca gtcatctccc
     2161 accgctccgg tgaatcagaa gatacgacaa tcgcagacat cgcagtagcg acaaatgcgg
     2221 gtcaaatcaa gacgggagca ccttcacgca cggaccgcgt cgcgaaatac aaccaattgc
     2281 ttc
//
LOCUS       DASULI010000291         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_9008, whole
            genome shotgun sequence.
ACCESSION   DASULI010000291 DASULI010000000
VERSION     DASULI010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9008"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFX34_06170"
     CDS             <1..191
                     /locus_tag="VFX34_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX5564525.1"
                     /translation="KPKTENKTKDDSKARTHIVAANETLYRIAMNYYGSDAGIEKIKK
                     ANGLSSNDIMAGQKLIIP"
     gene            373..1083
                     /locus_tag="VFX34_06175"
     CDS             373..1083
                     /locus_tag="VFX34_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5564526.1"
                     /translation="MFFYANRKNVIHHSVTVREGDTEGKELSVFFISDIHRRRIDGQL
                     LKKVRALGSIDIVIIGGDLAEGGVPLSCIDRNVEMLSRLAPLYFVWGNNDREVGEEEI
                     RSIIENHGGTVLENMDAAIENHPLWGISGTDDPSSGNVDVEAALKNVGNYTYLIVATH
                     TPSLFRKVEKLCRPELMVAGHMHGGQIRLGKYGLHPLGQFKEADGKAKLISNGYGTSI
                     VPLRFGAAPESHVITIKY"
     gene            1128..>2071
                     /locus_tag="VFX34_06180"
     CDS             1128..>2071
                     /locus_tag="VFX34_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HEX5564527.1"
                     /translation="MIYKDAIVIGGGPCGLSAAIELKNIGLDVLIIEKGNIVNSIYRY
                     PTHQTFFSSSIKLSIGDIPFITAKEKPKRNDALVYYRTVAELKGLAINSFEKVETVTK
                     EDGTFTVQTDLASYKAKFVIVATGYYDNPNSLDVPGDSLPKVLHYFKEAHPYFGRKVV
                     VIGGKNSAVDAAIELARAGASVTVIYRGSDYSPSVKPWILPGFDSLVRSGEIDMHFNS
                     IVTEITPSTVTFEKDGVRRTIENDTVFAMVGYHPDHDFLKRMGIRVDEESGRPCFDEN
                     TMESNVEGLYIAGVIAAGNNSNEIFIENGRWHGGLIAD"
BASE COUNT          604 a          397 c          523 g          547 t
ORIGIN      
        1 aaaaaccgaa aaccgaaaac aagaccaaag atgattcgaa agcgagaacg catattgtcg
       61 ccgcgaatga aactctttat cggattgcta tgaattatta tggttctgat gctggaatcg
      121 agaagattaa aaaagcaaac ggtctttcat ccaatgatat tatggcgggt caaaagttga
      181 ttattccgtg attgactcct aagacaggga agaaaaggca aagtcggttc tttgcctttt
      241 ttctttatcc cttattcatt ggaaagaagt tgcccactaa ttaaagtttc acaatatggc
      301 aggtgcagaa tgaaaattgt gttgaatagt ttagcgtgcg ttacagggat attggtgggt
      361 atccttgcat atatgttttt ttatgcaaac cggaaaaacg tcatccatca ttcagttacg
      421 gttcgagaag gcgataccga gggcaaggag ttgtccgtat ttttcatttc ggatattcac
      481 cgaagacgga ttgacggaca attattgaaa aaagtgcggg cgcttggatc gattgacatc
      541 gtcataatag gcggagatct cgctgaaggc ggggttccgc tttcctgtat tgaccggaat
      601 gtggaaatgc tttcccggct cgccccgctc tatttcgttt ggggcaataa cgaccgtgaa
      661 gtcggtgaag aggagatccg gagtattatt gaaaatcatg gcggtacggt gttagagaat
      721 atggacgctg caatcgaaaa ccatccgcta tggggaatta gcggcacgga tgatccttcc
      781 agcggaaatg tggatgtcga ggccgctttg aaaaatgtcg ggaactacac ttatctgatt
      841 gtagccaccc atacgccgtc tttattccgc aaagtcgaaa aactttgccg tccggagctg
      901 atggttgcgg gtcatatgca cggtggacaa ataagacttg gcaaatatgg attacatcca
      961 ttgggtcagt tcaaagaagc ggatggaaaa gcgaaattaa tcagcaacgg atatggcaca
     1021 tccatcgtcc cgctgcgatt tggcgcagct ccggaaagcc atgtgatcac gataaagtat
     1081 tagagtattg aagcaagttt tgtgcgattg taaggagact gatgagattg atttacaaag
     1141 atgcaattgt aatcggcgga gggccctgtg gtctttcggc cgctatcgaa ttgaagaata
     1201 taggtttaga cgtattgatt atagaaaaag ggaatattgt caactcgatt taccgatatc
     1261 cgacccacca gacctttttc agttcaagca taaagctgtc cataggagat atcccattca
     1321 taacagcaaa ggaaaagccg aaaaggaatg acgctctcgt ctattacagg acagttgctg
     1381 aattgaaagg ccttgccatc aatagttttg agaaagtcga aacggtgaca aaagaagacg
     1441 gcacttttac ggtgcaaact gatcttgcta gttacaaggc gaaatttgtc atcgttgcaa
     1501 ccggctatta cgataaccct aattccctgg acgtcccagg agattcattg ccgaaagtgc
     1561 ttcactactt caaagaagcg catccatatt tcggaaggaa ggttgtcgta atcggcggaa
     1621 aaaactccgc agtggatgct gcaatcgaat tggcacgcgc cggtgcttct gtgaccgtca
     1681 tttacagggg atcagattat tcaccaagcg ttaagccttg gatacttccc ggctttgaca
     1741 gcttggttcg aagcggtgaa attgacatgc atttcaattc gattgtcacg gagattacac
     1801 ctagtaccgt tactttcgaa aaagatgggg tacgcagaac gattgaaaat gatactgttt
     1861 ttgcgatggt cggttatcac cctgaccatg attttcttaa aaggatgggg attcgagtag
     1921 acgaggaaag cggtcgccca tgctttgatg aaaacacgat ggaatccaat gtggaaggtt
     1981 tgtatatagc gggagtaatc gctgcgggca ataattcaaa tgagatattc atagagaatg
     2041 gacgttggca tggcggtttg attgccgatc a
//
LOCUS       DASULI010000292         5917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1150957,
            whole genome shotgun sequence.
ACCESSION   DASULI010000292 DASULI010000000
VERSION     DASULI010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150957"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFX34_06185"
     CDS             <1..293
                     /locus_tag="VFX34_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564528.1"
                     /translation="DISSYIHRNPIDTRIPMVRSLEDYRHSSFPYYVDNQESQPRFLN
                     TELVPTFLPKPFETTSHAYQLYCLAYKQDINKVYEEMPPEEVYKFKRKLTNY"
     gene            complement(529..1299)
                     /locus_tag="VFX34_06190"
     CDS             complement(529..1299)
                     /locus_tag="VFX34_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564529.1"
                     /translation="MDIKEVTFSYDKKRDALHEIDSSIDIGKVTTIIGPNGCGKSTLL
                     GVLSNHLQPQIGDVLLDGKSLAEFKPKELARKLAVVHQQNSAPSDMTVEKLTAYGRIP
                     YKNLFSSSTDEDEKAIEWALESTNLLDMRHTPIDSLSGGQMQRVWIAMALTQQTPYLF
                     LDEPTTYLDIFYQYELLELIRSLNKQHGISIVMVLHDINQAIKYSDVIIVMKDGRIAA
                     KGNPQDIITADLIRDIYGVNVIVKHDPAAGTYIVPVGI"
     gene            complement(1289..2272)
                     /locus_tag="VFX34_06195"
     CDS             complement(1289..2272)
                     /locus_tag="VFX34_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017674005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5564530.1"
                     /translation="MNRKTISFIVVALLLAIVFVQSALTGSISVTPFELIHGLLTGTN
                     DDVAIIKDLRLPRILIAVFTGAALSVAGVLLQAVMRNPLADPGIIGVSAGASFMSVLV
                     IAMFPTLFFFVPLFAFIGGALAFFFVYSLSWKSGLDPLRMILIGIAVNALFTGLGQAI
                     GFSGGGLTQSMSQVTTSTLTMKKWSEVEVIALYGTIGLLLSFFVIRWCNYLSLEDRTA
                     KSLGVNVNLARFVISIIAVLLASIATTVAGMFAFVGLLVPHIGRTLVGNDHKVLIPFS
                     ALAGALLILLADTLGRTLIAPNEIPASIIMAVIGGPFLIFLLRKSDRIYGH"
     gene            complement(2296..3174)
                     /gene="isdE"
                     /locus_tag="VFX34_06200"
     CDS             complement(2296..3174)
                     /gene="isdE"
                     /locus_tag="VFX34_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006637679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEX5564531.1"
                     /translation="MKKAIGLFGLLVSVMLAGCSASAGDNANEIGEQDGDRIVATTVA
                     LTEIMDALDIDLVGVPSSYKELPERYAEAKEVGNPMSPDMETLLSLKPTEIYSVTTLK
                     YDLQEMFEERGIDMTYANLESIDAMHEEILKIGEKYDRMGQAEAIVAQFEEKSAAIEK
                     AIEGKDKPSVLILMGIPGSYLVATEHSYIGDLVKRAGGVNVITGEDVEFISSNTEYLQ
                     QTNPDVILRAAHGMPEQVIEMFDKEFRTNDVWKHFDAVKNGRVYDLEETLFGTTGHLA
                     AAEALDELMNMLHEAK"
     gene            complement(3190..4137)
                     /locus_tag="VFX34_06205"
     CDS             complement(3190..4137)
                     /locus_tag="VFX34_06205"
                     /inference="COORDINATES: protein motif:HMM:NF016890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEAT domain-containing protein"
                     /protein_id="HEX5564532.1"
                     /translation="MKRNLVFAMVFAVVIVLFIPVMPLTAAAATFADGEYSVPFTVLK
                     DTSNERSTTADYMVSPAKVQLVNDKATVTVTLKNSSWWQYFKVQNGGSFSDVQVVSEA
                     GDQRVVRFTVSDLTKMVNAKIHIIVTGIPGFEYDNKYDIRFNFNTSSIPAPPVEKPAA
                     TPPPVKETQPETPKKTEAKPVQRQEQKTVQKQEQKPAQKPAVNKTETAKSTESVTKKE
                     EKLTVNETGKDKAAEMEDGVTDKEESEKAAEDADAEEADAVNEEELEELEEAAIEEAA
                     VEEVKDEKSSNTFFIVIVVIGALIGAAVVFAIARKRARK"
     gene            complement(4207..5649)
                     /locus_tag="VFX34_06210"
     CDS             complement(4207..5649)
                     /locus_tag="VFX34_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEAT domain-containing protein"
                     /protein_id="HEX5564533.1"
                     /translation="MKKNLMVLFTAMLLIFTAMPALPAAAETAAAQEASYELPFTVLH
                     ENKDEVSVAGKYVKTPAQVKVEDGKAFVYMTLLDSQYWQSLKIQNGDKFVDVEIVSEN
                     KEEQTRLVKFELIDVKKTLNAKAHIIVTGIPGFEYNETHNIRFQFDASKAPAENEEAP
                     ETPETPAASVKDGDYTVGFNVLHEKEDKESSMTRYIEAPAALSVKDGKQVVTIKLTNN
                     EQITEFQVEQDGGFVDATVVSVDEEANRRTVSFEVADLSKIIDAKVAVSVPAMNHNAN
                     YTIRLAFDKDSLKPVAEEVKVTFKDINNTFAKPYIEALAAKQIVKGKTADTFAPNDNM
                     TRAEFARIISRALNLEKKEYQGTFTDLTKGTSYVYEVEAANRAGIILGDAGKFNPNAQ
                     ITRQEMVAMIVRSIEYKNASLVKNVDTSVKFADAGSVSNYAKEAVQLAAGLGIVDGNV
                     VNGQKVFEPKATATRAHAVKMLYYMLETIK"
     gene            complement(5667..>5917)
                     /locus_tag="VFX34_06215"
     CDS             complement(5667..>5917)
                     /locus_tag="VFX34_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564534.1"
                     /translation="NVVGELAEPFPVKMHVVIDELEIPYDHAYTVRVAVDADSLQETD
                     QEWIDSVSEGSTIWFWIATAIVIAAMIFVALRLFRSKK"
BASE COUNT         1423 a         1640 c         1252 g         1602 t
ORIGIN      
        1 tggacattag tagctacatc catcggaatc cgatcgacac gcggattccg atggtgagat
       61 cgttggaaga ttatcgacac agctcatttc cctactacgt tgataatcag gaatcacaac
      121 cacgcttctt gaacactgag cttgtaccga catttttacc gaagccattc gagacaacaa
      181 gccatgcgta ccaactttac tgtttagcgt acaaacaaga catcaataag gtgtacgagg
      241 aaatgcctcc cgaagaagta tataaattca aacggaagct gaccaattat tgaattggtc
      301 agctttctct ttttcattta tacaattgta gtgtattgtc tgaattgttt tggtgcctgt
      361 gtaagacgca tttatgacaa ttcactatcc ggcatattaa tacacacgaa aactaatcaa
      421 ataatgttat aaacggcttt ctgtttttca aatatacaat tgtagtgtat tatctgaata
      481 gtgcgctaca caggcaccgt caaaaggcac cgccaaaaat ggaatagatc aaatgcctac
      541 gggtacgatg taggtgccgg cggcggggtc atgtttgacg atgacgttga cgccgtagat
      601 gtcgcggatc aggtcggctg tgatgatgtc ttgcgggttg cctttagcgg cgatgcggcc
      661 gtctttcatg acgatgatga cgtcactgta tttgatggcc tggttgatat catgcaatac
      721 catgacgatg gagatgccat gctgtttatt cagactgcgg atgagttcga gcagctcata
      781 ttgatagaaa atatccaagt acgtcgtcgg ttcatccaga aacagatagg gcgtttgctg
      841 ggtcaaagcc atggcgatcc agacgcgctg catttggccg ccggataggc tgtcgatcgg
      901 cgtatgccgc atatcgagca agttcgtgct ttcgagcgcc cattcgattg ccttttcgtc
      961 ttcatcagtc gaggaagaga agaggttctt atacggaatc cgcccatacg ccgtcagctt
     1021 ttccaccgtc atatcggatg gcgcgctgtt ttgctggtgg acgaccgcaa gcttccgggc
     1081 aagttccttc ggcttgaact ccgccaacga tttcccgtcc agcaacacat ccccgatttg
     1141 cggctgaaga tggttggaca ggacgcctag caaagttgat ttcccacatc cattcggccc
     1201 gatgatcgtc gtcactttgc cgatgtcaat cgagctgtca atctcgtgca gcgcatccct
     1261 cttcttgtcg tatgaaaatg tgacttcctt aatgtccata aatgcgatca ctctttctca
     1321 gcaagaatat aaggaatggt ccaccgatca ccgccatgat gatggatgca ggaatttcat
     1381 tcggcgcgat caacgtcctt ccaagcgtat ctgccaataa aatcagcaat gcgccagcca
     1441 acgcagaaaa cgggatcagc actttatggt cattgccgac aagcgtcctt ccgatatgcg
     1501 gcaccaataa cccgacaaac gcaaacatgc ccgcaaccgt cgtcgcaatt gaagccaata
     1561 aaaccgcaat gatcgaaatg acgaacctcg ccaaattcac attgacgccg aggcttttcg
     1621 ccgtcctgtc ctcaagcgac aaataattgc accatctaat tacgaaaaac gacagcaaca
     1681 acccgatcgt cccgtaaagc gcgatcactt ccacttcgct ccattttttc atcgtcagcg
     1741 tcgacgtcgt cacttggctc atcgactgcg ttagaccgcc accgctgaac ccgatcgcct
     1801 gcccgagtcc agtgaacagc gcgtttaccg caatcccgat cagaatcatc cgcagcggat
     1861 ccaatcccga cttccacgat aatgaataca cgaagaaaaa cgccagcgca ccgccgataa
     1921 acgcaaacag cggtacgaaa aagaacaatg ttgggaacat cgcaatcacc agtacagaca
     1981 tgaagcttgc acccgcggaa acgccgatga tcccgggatc cgccaacgga tttcgcatga
     2041 ccgcctgcag caaaacgccc gccacggaca atgccgcccc cgtgaatacc gcaatcaaaa
     2101 tccgcggcag ccgcaagtct ttaatgatgg cgacgtcgtc attcgttccc gtcaacagcc
     2161 cgtgaatcaa ttcgaaaggc gtcaccgaaa tgctgcccgt caacgcagat tgaacgaaaa
     2221 caatcgccaa aagaagtgcc acgactatga aactgatggt tttccgattg attcccgcca
     2281 tgattttacc tgccttcatt tcgcttcatg caacatattc atcaactcat ccaacgcttc
     2341 cgccgcggcc aaatggcctg tcgtcccgaa cagcgtctcc tccagatcat agacgcgccc
     2401 gttcttcacc gcatcgaaat gcttccacac atcattcgtg cggaactcct tgtcgaacat
     2461 ctcgataacc tgctcaggca tgccgtgtgc cgcccgcaaa atgacatccg gattcgtctg
     2521 ctgcaaatac tccgtattgg aggaaatgaa ctcaacatcc tcgcccgtaa tgacattgac
     2581 accgccagcc cgcttcacga ggtccccgat gtatgaatgc tccgtcgcca ccaaatagct
     2641 tcccggaatc cccatgagaa tgagcacgga aggcttatcc ttcccctcga ttgccttttc
     2701 gattgcagca gatttctcct cgaattgcgc cacaatcgct tccgcctgcc ccatccgatc
     2761 gtacttttca ccgatcttca agatttcttc gtgcatcgca tcgatgcttt cgaggttcgc
     2821 atatgtcata tcgatcccgc gttcctcaaa catttcttgc aagtcatatt tcaacgttgt
     2881 caccgaataa atttccgtcg gcttcaacga cagcagcgtt tccatgtcgg ggctcatcgg
     2941 attgccgact tccttagctt cggcgtagcg ctccggcaac tccttgtagc tcgacggaac
     3001 gccaaccaaa tcgatgtcta gcgcgtccat gatttccgtc agcgcgacag ttgttgcaac
     3061 gattcgatcg ccgtcctgct cgccgatttc attcgcatta tccccggcac tcgcagaaca
     3121 gccggcaagc atgacactta ccaacaagcc gaaaaggccg atcgcttttt tcattccagc
     3181 acaactccct tattttcgag cacgtttcct agctatcgcg aatacaaccg cggcaccaat
     3241 gagagcacca atcactacga tgacaatgaa gaatgtattc gaggatttct catccttgac
     3301 ctcttcaacc gccgcctctt cgattgcggc ttcttccagt tcttcaagtt cttcttcatt
     3361 cacagcatca gcttcttcag cgtcagcgtc ctctgccgct ttttccgact cttctttgtc
     3421 ggtcactccg tcttccattt ccgccgcttt atcttttccg gtttcattta cagtcaactt
     3481 ctcttctttt ttcgttacac tttccgtcga tttggctgtc tcggttttat tcacggcagg
     3541 cttctgggct ggcttttgtt cctgcttttg aaccgtcttt tgttcctgcc tttgaaccgg
     3601 ctttgcctca gtcttcttcg gcgtttcagg ttgtgtctcc ttcaccggag gaggcgtcgc
     3661 tgccggcttc tctaccggag gagccggtat ggacgacgta ttgaaattga agcggatgtc
     3721 gtatttattg tcatattcaa accccgggat gcctgtcacg atgatgtgga ttttggcatt
     3781 gaccattttc gtaaggtcag acaccgtaaa ccgcacaaca cgttgatccc ctgcttcgct
     3841 caccacttgc acatccgaaa aactgccgcc gttctgcacc ttgaaatatt gccaccatga
     3901 gctgtttttc aacgtgaccg tcaccgtagc cttgtcattc accagctgga ccttcgcagg
     3961 gctgaccatg taatccgctg tcgtcgaccg ctcgttgcta gtatctttca gcacagtaaa
     4021 cgggaccgaa tattccccgt ccgcaaacgt tgcagccgcc gcagttaacg gcatgactgg
     4081 aatgaaaagc actatgacca ctgcaaagac catcgcaaaa actaggtttc ttttcatttc
     4141 aaactccttt attctacaca caacgccggc tgtcacaaag tgcgtgacgc cggcatcaga
     4201 gtggaattat ttaatcgttt caagcatgta gtaaagcatt ttcactgcat gcgcacgcgt
     4261 cgcggtcgct ttcggttcaa acactttttg gccgttcaca acgttgccgt cgacgatgcc
     4321 aaggccagct gccaattgaa cagcttcctt cgcgtagttg ctcacgctgc ccgcatccgc
     4381 aaatttaaca gacgtgtcaa cgtttttcac gagtgaagca tttttatatt cgattgaacg
     4441 gacaatcatc gcaaccattt cctggcgagt gatttgtgcg ttcgggttga atttcccggc
     4501 atcaccaaga atgattccgg cacggttcgc cgcttccact tcgtacacgt aacttgtacc
     4561 tttcgtcaag tcagtgaacg tcccttggta ttccttcttt tcaagattca atgcgcggga
     4621 aataatgcgg gcaaattccg ctctcgtcat gttgtcgttc ggcgcaaacg tatctgccgt
     4681 tttacctttt acgatctgct tcgcagcaag agcttcgatg taaggttttg caaatgtgtt
     4741 gttaatatct ttgaatgtaa cttttacttc ttctgctacg ggcttaaggc tgtccttatc
     4801 gaatgcaagg cgaattgtgt aatttgcatt atggttcata gccggaacgg aaaccgccac
     4861 tttcgcgtca atgatctttg agagatcagc tacttcgaat gataccgtac ggcggttcgc
     4921 ttcttcatcc acgcttacaa cagtcgcatc gacgaatccg ccatcttgct caacttgaaa
     4981 ttcagtgatt tgctcgttgt ttgtcaattt aattgtaacg acttgtttgc cgtcttttac
     5041 ggacaatgcc gccggagctt caatgtagcg tgtcattgaa gattccttat cttctttctc
     5101 atgcaataca ttgaaaccta ccgtgtaatc gccgtcttta acggatgctg ctggtgtttc
     5161 aggagtttct ggtgcttctt cattctcagc tggagctttg cttgcatcga actggaaacg
     5221 gatgttgtgc gtttcgttgt attcaaatcc aggaatgcct gtcacgataa tatgcgcctt
     5281 tgcattcaat gtttttttga catctattaa ttcaaacttc acaaggcgag tctgctcttc
     5341 tttgttttcg ctcacaattt caacatctac aaatttgtcg ccattttgga ttttcaatga
     5401 ctgccaatat tggctatcca aaagcgtcat gtagacgaac gcctttccgt cttccacttt
     5461 cacttgtgca ggggttttca catatttacc tgcaacagaa acttcgtcct tattttcatg
     5521 cagcacggtg aacggaagct cataagacgc ctcctgtgca gccgccgttt cagcagccgc
     5581 cggaagtgca ggcattgccg taaagatcag caacatcgct gtgaataaaa ccatcaaatt
     5641 cttcttcatt ctagtaatct ccttttctac tttttcgacc ggaacaatcg cagagcaaca
     5701 aaaatcatcg ctgcaataac gattgcagtc gcaatccaaa accagattgt acttccttcg
     5761 ctaacagagt cgatccactc ctggtccgtc tcctgcaagg aatcagcgtc caccgcaact
     5821 cgaacggtat acgcatggtc atacgggatc tccaactcat caatcaccac atgcatcttc
     5881 accggaaacg gctcggccaa ctcgccgacc acgttca
//
LOCUS       DASULI010000293         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1604222,
            whole genome shotgun sequence.
ACCESSION   DASULI010000293 DASULI010000000
VERSION     DASULI010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604222"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..718
                     /locus_tag="VFX34_06220"
     CDS             101..718
                     /locus_tag="VFX34_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HEX5564535.1"
                     /translation="MNQQNCGTSNIAGASFSLHPMSDDFIDIIKSALQEVDTSKVWMK
                     TDDVTTTVRGKMVHIFDVTRAIFLHAAKSGKHVAFQATYSLGCPGDSESDVYLAEDDT
                     PANAAASESIQQYIAAKFSLYPLGSGSYMDVIYQQIEAIKEYATVTPAHYSTKLEGDA
                     FKIFDGLHTVFEEVVNAGSRHTVMTVTISANSPSHRGDQHENMAL"
     gene            699..1277
                     /locus_tag="VFX34_06225"
     CDS             699..1277
                     /locus_tag="VFX34_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX5564536.1"
                     /translation="MKTWRFKEIVVLSALSVVFAIVYLAFLPLGKLLFGFFGPIGYDL
                     IFGIWFIVSIIAAYIIRKPGAAFLSETIAAMVEVLIGNAVGPMLIVVGMVQGIGAEAV
                     FAATRWRLYSTWVLMLAGVGSAVTSFIYGYFMSGYAALGAGYVTAMFVTRMISSALIA
                     GLLGKAISDGLAKTGVLSSYALGKEVRKRKMA"
     gene            1274..>2568
                     /locus_tag="VFX34_06230"
     CDS             1274..>2568
                     /locus_tag="VFX34_06230"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEX5564537.1"
                     /translation="MTPVITVKDLSLRFEFAERDTLSGVSFSLERGESILVLGPSGSG
                     KSTLTLCLNGLYPRELDGSMSGDILIHGKPTTSYEPGQLSRHVGVVFQDPETQFCMLT
                     VEDEVAFGLENIGVPHADMVDRIDQALRLVNMQEFKQSMIATLSGGQKQKLALACILA
                     LEPEVLILDEPTANLDPIARIDVIQTIKRLQEELGFTLLIIEHQLDQWTDLADRCLLF
                     NQTGMIFFDGSLQEAIRTNLPKIKQEGIWLPNVAQHVLPYADPTTQTIPLTLEEFSRQ
                     AHVWKGIEWIKCERNVIPTKEKVFLETKNISFSVGEKSILKEISISLRENEFVAIVGA
                     NGSGKTTLSRVLAGLQKPTEGSVALHGKPLHSWREADLRNEIGYVFQNPEHQFITNTV
                     YEEVAFSLQIKGLPENEIRERVNRILERYGLAAYVDEHP"
BASE COUNT          686 a          571 c          640 g          671 t
ORIGIN      
        1 tcatactgac tataaacgct aactgcattc caccttggat gcagtttttt tgtgtctttg
       61 gccaaagcac atcaatcaaa acaaatgagg aggaagtaga atgaatcaac aaaactgtgg
      121 aacgagtaac attgcgggcg caagcttttc cttgcatccg atgagcgatg actttattga
      181 cataataaaa agtgcacttc aggaagtgga tacgtcaaag gtatggatga aaacagatga
      241 cgtgacaaca acggtgcgcg gtaaaatggt tcatattttt gatgtcacac gcgcaatttt
      301 tctccatgca gccaagtcag gtaagcatgt tgcgtttcaa gcgacatatt cgctcggttg
      361 tcctggggat tccgaaagtg acgtctatct agcggaagac gatactcctg ccaatgcggc
      421 tgcgtctgaa tcaattcaac aatacattgc ggcaaagttt tctctctacc cattagggag
      481 cggttcctat atggatgtta tttatcaaca aattgaagca atcaaagaat acgcgacggt
      541 cacacctgct cattactcaa caaagttgga aggggacgcc tttaaaatct tcgatggcct
      601 gcacacggtc ttcgaagagg ttgtaaatgc cggctcgcgt cacacggtta tgactgtgac
      661 aatttcagcg aatagtcctt cccatcgagg tgatcaacat gaaaacatgg cgctttaaag
      721 agattgttgt tctctccgcc ttatccgtcg tctttgcaat cgtctacttg gcgtttctgc
      781 ctcttggaaa attactcttc ggattttttg gcccgattgg ctacgatttg attttcggaa
      841 tctggtttat tgtttcgatc atcgctgcct atattatccg gaagccggga gcggccttct
      901 tgtccgaaac gatagctgcc atggttgagg ttctgatagg aaatgcggtc ggcccgatgc
      961 tcattgttgt cggtatggta caaggaattg gtgcagaagc ggtctttgcc gctacgagat
     1021 ggagacttta ttccacatgg gttctgatgc ttgcaggggt tgggtctgca gttacgagct
     1081 ttatctacgg atactttatg agcgggtatg cagcacttgg agctggctac gtgacggcaa
     1141 tgtttgtcac tcgcatgatt agcagtgcct taatcgccgg acttctcgga aaagcgatta
     1201 gtgatggact cgctaaaaca ggggtgcttt cgagctatgc gctcgggaaa gaagtgcgaa
     1261 agaggaagat ggcatgacgc ctgttatcac agtaaaggat ctctctttgc gattcgaatt
     1321 cgcagagagg gataccttgt cgggcgtttc cttttcactc gaacggggtg aaagcattct
     1381 tgtactcggt ccgagcggaa gcggaaaaag cactctaacc ctttgtttga atggactcta
     1441 tccccgtgaa ctagatggct cgatgtccgg agacattttg attcacggaa agccgacaac
     1501 atcctatgaa ccgggacagc tcagccgtca tgtcggcgtt gtctttcaag atcctgagac
     1561 gcagttttgc atgctgaccg ttgaggatga agtggcgttc ggattggaaa acataggggt
     1621 ccctcatgca gacatggtgg accggattga ccaggccctg cgtctcgtca atatgcagga
     1681 gtttaaacaa tctatgatcg ctactttgtc cggtgggcaa aagcaaaagc ttgcactcgc
     1741 ctgcattctt gcgctcgaac ccgaagtatt gattctggat gaaccaacag caaatcttga
     1801 tcctattgcg agaatcgatg ttatccaaac gataaaacgg ttacaagagg aactgggctt
     1861 cacccttctc attatcgagc atcaactgga ccaatggaca gacttggcag accgatgtct
     1921 tttattcaat caaaccggta tgattttttt cgatggatcg ctccaagagg caattcggac
     1981 gaatctaccg aaaattaagc aagaaggaat ctggctaccg aacgttgcgc aacacgtatt
     2041 gccttatgcg gatcctacga cccagacaat cccccttacg ttagaggaat tctcacgcca
     2101 agcgcatgtc tggaaaggca ttgagtggat caagtgtgaa cggaacgtaa ttccaactaa
     2161 agaaaaggtg ttccttgaaa caaagaacat ttccttttcg gtcggagaga aatccatttt
     2221 aaaagagata tccatctcac tacgcgaaaa cgaatttgta gccatcgtcg gagcaaacgg
     2281 cagcgggaag acaaccctct cccgcgtact ggcaggttta cagaagccga cggaagggtc
     2341 tgttgcctta catgggaaac cgctccacag ttggcgagag gctgatttgc gcaatgaaat
     2401 cgggtatgta ttccaaaatc ctgagcacca atttattacg aatacagtgt atgaagaagt
     2461 tgccttcagt cttcaaataa aagggttgcc ggaaaacgag atccgtgagc gcgtcaaccg
     2521 gatcctcgaa cggtacggat tggctgctta cgtggatgaa catccgta
//
LOCUS       DASULI010000294         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_394714, whole
            genome shotgun sequence.
ACCESSION   DASULI010000294 DASULI010000000
VERSION     DASULI010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394714"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFX34_06235"
     CDS             complement(<1..578)
                     /locus_tag="VFX34_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564538.1"
                     /translation="MANDNFEKRMEFLKKSYERVPASFDPDEVFRKIDQEEVPQPEKQ
                     KPSKGGIKQTFTIWAMGIASLFILGLIGTGYVFEQKQNVKEDTVDSAELDAYIEDLKA
                     KYEVEKEKRREMLKLDKEHFELYAGSSSISLLSMDSFIKTAKQNGNAKEIVLNQYNRA
                     VDQLKLPSEMIQELKRSPLTDDEAGSIGFIGT"
     gene            complement(568..1122)
                     /locus_tag="VFX34_06240"
     CDS             complement(568..1122)
                     /locus_tag="VFX34_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5564539.1"
                     /translation="MEEADLIARAQNGEREAFAQLMNLHYRTVEKFSYQCGVRLDDIP
                     DVTQEVFIKLYRFLPQFNQQRFTTWLYKITLNTARDYYRKEGRETAKEDRMKKAGSAH
                     AQQSAEFQVLLFEEDRELHAAILQLDEKYRIPLILFYFQDLTYQQIADVMNISLATVK
                     TRIFRAKDSLKKEMDMKGGVTHGQ"
     gene            complement(1226..1486)
                     /locus_tag="VFX34_06245"
     CDS             complement(1226..1486)
                     /locus_tag="VFX34_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1871 family protein"
                     /protein_id="HEX5564540.1"
                     /translation="MQTIEMNKKAIMLLEEWDPFNVGRKAYELEIADVVGELQVLDHP
                     TDLAKRIREIYEHSYEIWIPLENCMQIAYKLLAIKYEAKCIV"
     gene            1588..2742
                     /locus_tag="VFX34_06250"
     CDS             1588..2742
                     /locus_tag="VFX34_06250"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HEX5564541.1"
                     /translation="MIDRKNTRSVKWGNMEAVYGVEDASEVLPMWIADMDFKAPQPVI
                     AAMQERLDHGVFGYSYICAKCKDAVRTWLSSRHGWETENDWMLFHHGVVPAIATVVET
                     FTNAGDNILITSPVYPPFFNVPGHQKRNIVECRLREENGNYSIDFDAFEKSLQQGVKL
                     FILCHPHNPGGVVWTEEELRNILRLCKKYDVLILSDEIHADLVLPGFKHIPLAVLAEE
                     EGGRVITCVAPTKTFNLAGVQAAVMISKEDDIREALQQSALAHGQMELNAFAASALIS
                     AFTECEEWLENLLETISSNMDFVINTLTEAVPGIQIQKPQATYLLWIDYRGTGLSEEE
                     MMDRLLNKGKLALEPGSKYGEAGLGFLRMNVATPRLIVEDGVKRFIKAMQ"
BASE COUNT          758 a          659 c          612 g          888 t
ORIGIN      
        1 taagtgccaa tgaaaccgat gcttccagcc tcatcatccg tcagtggact acgctttaat
       61 tcctgaatca tttctgatgg taatttcaat tgatccaccg ctcgattata ttggttcaat
      121 acgatctctt ttgcgtttcc attctgtttc gccgttttta taaaactgtc catcgaaagc
      181 aatgaaatgg aacttgatcc ggcatacaat tcgaaatgtt ctttgtccaa cttcagcatt
      241 tcccggcgct tttccttttc cacttcgtac ttcgctttca agtcctcaat atatgcatca
      301 agctctgctg agtcgactgt atcctctttt acattctgct tttgctcaaa aacatatccc
      361 gttccaatga gaccgagaat gaatagactc gcgatgccca ttgcccaaat cgtgaaggtt
      421 tgcttaattc caccctttga aggcttttgc ttctccggtt gtggaacttc ctcctgatca
      481 atcttcctga aaacttcatc cggatcaaat gaggcgggaa cacgttcata tgactttttc
      541 aaaaattcca tccgtttctc aaaattgtca ttggccatgt gtcacccctc ccttcatgtc
      601 catctctttt ttcaagctgt cctttgctct gaaaatccgg gttttcactg ttgccaacga
      661 tatattcatg acatctgcga tttgctggta cgttaagtct tgaaaataaa acaagatgag
      721 aggaatccga tatttttcat ccaattgcaa gatggctgca tgcaattcgc gatcttcttc
      781 aaaaaggaga acttggaatt cggctgattg ttgagcatgt gcactgccgg cttttttcat
      841 ccgatcctct ttcgctgtct ccctgccttc tttccgataa taatcgcgcg ctgtatttaa
      901 cgtaattttg tataaccatg tcgtaaacct ctgctggttg aactgcggca ggaaacgata
      961 cagcttgata aacacttctt gtgtcacatc cggtatatca tcaagacgaa caccacattg
     1021 atatgaaaac ttctcgacag ttctataatg caaattcatc agttgggcaa atgcctccct
     1081 ctccccgttt tgcgcacggg cgatcaaatc tgcttcttcc ataatgcccc ctcactctct
     1141 atctcctctg atgatgatac gaaaggaatc cccgtaatgt ttcaactaaa aaaccgcatc
     1201 tacattttcg tagatgcggc cattgttaga ctatacattt cgcttcatat tttatcgcca
     1261 ataacttata agctatttgc atgcaattct ctagtggaat ccaaatttcg taagaatgtt
     1321 cataaatctc acggattcgt ttcgcaagat cggtcgggtg atcaagaact tggagctccc
     1381 caacgacatc ggcaatttca agttcatatg cttttctgcc cacattgaaa ggatcccatt
     1441 cttcaagcag catgattgct tttttattca tttcgattgt ttgcacgacg agtcacctta
     1501 tttttttctt ttctttcatt tatgatagca taggaatgga aaagaaaaaa ggaaatgagg
     1561 gattttggca tgaaagagtt cacacacgtg attgatcgaa aaaatacccg ttcagtgaaa
     1621 tggggcaata tggaagctgt ttacggggtg gaagacgctt cggaagtttt accgatgtgg
     1681 attgcagata tggatttcaa ggctccgcag ccagtcattg cggccatgca agaaaggttg
     1741 gaccatggtg ttttcggcta ttcgtacata tgtgccaaat gtaaagatgc tgtccgtact
     1801 tggctatcat cccgccacgg atgggaaact gaaaatgact ggatgctttt ccatcacggt
     1861 gtcgttcctg caatcgctac ggttgtggag acgttcacga atgccggcga taatatttta
     1921 attacctcgc ccgtgtatcc gccattcttc aacgtgcccg ggcatcaaaa gcggaacatt
     1981 gtcgaatgta gattgcggga agagaacgga aattactcca tcgacttcga tgcatttgaa
     2041 aaaagcttgc agcaaggtgt taagctgttc atcttatgcc atccgcacaa ccccggaggc
     2101 gttgtttgga cagaagagga attgcgcaac atcctccgtc tatgcaagaa atatgatgtg
     2161 ttgatccttt ctgatgaaat tcatgccgac cttgtcctgc ctgggtttaa gcatattcct
     2221 ttggcggtgt tagccgaaga ggaaggcgga cgggtcatca cttgtgtcgc tccgacaaag
     2281 acgttcaact tggcgggcgt acaagcagca gtcatgattt caaaagagga tgacatccgg
     2341 gaagctttac agcaaagtgc gctggctcat ggccaaatgg aattgaatgc atttgccgct
     2401 tcagctctta tttccgcatt caccgagtgt gaagaatggc tggagaactt gcttgaaact
     2461 atttcaagca atatggattt tgtcattaac acattaacgg aggctgttcc gggtatccag
     2521 atccaaaaac cgcaagcgac atacttattg tggatcgatt accgcggcac ggggctttcg
     2581 gaagaagaaa tgatggatag gctattgaat aaaggaaaac ttgcgctcga gccagggtct
     2641 aagtatggtg aagctggctt aggattcctt cgtatgaatg ttgcaactcc tcggttgatt
     2701 gtcgaggatg gggtgaaaag attcattaaa gctatgcaat gaataataaa aatgcatgat
     2761 ccttataatc ggatcatgca tttttcatta aagttcttgt ttttctcttg cttcgcgacg
     2821 ttcgcgcaag atgcgttctt ccaactcgcg tgtttcctct tcttgctttt tcgaattccg
     2881 tctggtccac gaaaatgtga gcacaagaag aatcatg
//
LOCUS       DASULI010000295         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_850088, whole
            genome shotgun sequence.
ACCESSION   DASULI010000295 DASULI010000000
VERSION     DASULI010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850088"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFX34_06255"
     CDS             complement(<1..457)
                     /locus_tag="VFX34_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX5564542.1"
                     /translation="MRSKRWRKRLNGFLAAGLAVTMVVPSVPAIVKAESVASDLFISE
                     YVEGSSFNKAIELYNGTGQAVDLSGYTLELYSNGGTTVSARMTLSGTLNNGETYVLYH
                     KDAAAGIKEKGDLENTTVINFNGDDALVLKKGNTVIDSFGQVGARENWGL"
     gene            complement(988..2733)
                     /locus_tag="VFX34_06260"
     CDS             complement(988..2733)
                     /locus_tag="VFX34_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5564543.1"
                     /translation="MKPNSKFATKIVVLVVLVIGIAASVGWYVVSGANKGMNVSFSEF
                     EKTIQAQEGKPLSLKEKADGTLSLKTNDGLYVTQVRPQSQLAEQLVEQYNLSYTFADA
                     GQYNGLFVGGLIIASFLTLMILHKKGKLGVGVSSMKNGTSKATPLPEITLDDIGGLPE
                     EMKEEIHQTLSIIKDPKRATQVGIQAPKGLLLYGPPGTGKTLLAQAIAREIGATFYSS
                     SGSAFTELFVGVGASRVRTLFQNARKHRPAVIFIDEVDALAGKRKEHGGEESEKTLTE
                     LLVQLDGGNDNEGILFIAATNRKDMLDEAFLRPGRIDYTFNVPLPDTHGRREIIDIHT
                     KGRLLAEEVYASLDVLAESTSGFSGAELSSLFETASKRAIRDGREKIGKGDLDFAIDR
                     TILGSTSRTLNDPDTKRRVAIHEAGHALVAALTKPGSVRKATIIPRGQALGYVAPIQK
                     ELHLQTASELLDQVSMILAGGVAERLYLGEHSIGVSGDVQQAKDIIERMVDTGLLQDG
                     FMLTFNKGEKELKMQALFGEALKQTETLIQENAAQFEELVNVLFQKETLDGSEVQEIV
                     DGKGVEEVKELVIIV"
BASE COUNT          670 a          733 c          615 g          806 t
ORIGIN      
        1 ccaatcccca attctcacga gcaccaactt gaccaaatga atcaatcact gtattgcctt
       61 ttttgaggac aagtgcatca tcaccgttga aattgatgac agttgtattt tcaagatcgc
      121 ccttttcttt aatccccgca gctgcatctt tatgatataa aacatatgtc tcgccattgt
      181 tcaatgtacc ggataatgtc atcctggcag atacagtagt tccaccgttg gaatatagct
      241 ccaacgtata accactcaaa tccacagcct gtcccgttcc gttataaagc tcaatcgcct
      301 tattgaagct gctgccttct acatattcgg aaatgaacag atccgatgca accgattctg
      361 ctttcacaat cgccggaacc gatggcacga ccatcgttac tgctaatcca gctgctaaaa
      421 agccattcaa gcgctttctc catctcttac tccgcatgaa accattctcc tttcaatttt
      481 gaaaaatatg gtgcagtgcg tgaagatcct ccgtaaaccg ctccctttcc acataagttt
      541 ggtagattaa tagactgcat tcccatcgta ccattgcatg tatggcatga ccatgagact
      601 ttttgcaata ttttgcgatt attacatcaa actttcgaca tggattttcg aagggattgg
      661 gtgagaatag ttggagaaag tgattggttg tgttgcgcgg gatgtcggtt gcgatacgag
      721 aggtattcgt ttcgttgcac tgtaagttga ttgtattggg aagtactctg gttgcgttgc
      781 ccatatttga ttgctttgcg agctggattg atttcgttgc gtccgtgttt ggttgcattg
      841 taccagtttg cttgcgttgc gggcaggggt gattgcattg cgcctgcaat tccttgcgtt
      901 ccaccgcaag tttattgtgt tgcaagaaat ccgttccatc ccacaaaaaa ctccccagcc
      961 cattcggact gaggagtctt tcttttatta cacgatgatg acaagctctt ttacttcttc
     1021 tacacctttg ccatcgacga tttcctgtac ttcggatccg tccaatgtct ctttctggaa
     1081 gaggacattg accaattcct cgaactgcgc ggcattttcc tggatgagtg tttcggtttg
     1141 tttcaatgcc tctccgaaga gcgcctgcat tttcaactct ttttcgcctt tattaaacgt
     1201 cagcataaac ccatcttgaa gcaggccggt atcgaccatc cgctcgatga tgtctttcgc
     1261 ctgctgcaca tccccgctga cgccgatgct atgctcaccg aggtaaagcc gctccgcgac
     1321 gcctcccgcc agaatcatgc tcacttgatc gagcagttcg ctcgccgtct gcaaatgaag
     1381 ctctttctgg atcggcgcca cgtatccaag cgcttgtcca cgcggaatga ttgtcgcttt
     1441 ccgtacagaa cccggttttg tcaacgcagc gaccaaagca tggcccgctt cgtggattgc
     1501 gacccttctt ttcgtatcag gatcgttcag cgtacgtgat gtgctgccga gaatcgtccg
     1561 gtcgattgcg aaatcgaggt ctcctttgcc tatcttttca cggccatcac ggattgcccg
     1621 cttgctggcc gtttcgaata aagagcttag ctcggctccg gaaaaaccgg acgtgctttc
     1681 agccaataca tccaatgaag catacacttc ttccgccaac aaccgtcctt tcgtatggat
     1741 gtcgatgatt tcacgacggc catgcgtgtc cggaagcggt acattgaacg tatagtcgat
     1801 acgccctgga cgaaggaatg cttcatcgag catatctttc cggttcgttg ccgcgatgaa
     1861 aaggattcct tcattatcgt tgccgccgtc caattgaacg agaagctccg tcaatgtctt
     1921 ctcggattcc tctccgccat gctctttccg tttgccggca agcgcgtcga cttcatcgat
     1981 gaaaatgacg gctggacggt gtttgcgggc attttggaac aatgtgcgca cacgagacgc
     2041 accgactccg acaaacagct ccgtaaatgc ggaaccgctg gatgaataga aggttgcccc
     2101 aatttcacgg gcaatcgctt gggcaagcaa tgtcttcccg gttcctggag gtccgtagag
     2161 aaggagccct ttcggtgcct ggatcccgac ttgcgttgcg cgtttcggat ctttgatgat
     2221 cgacagcgtc tggtgaatct cttccttcat ctcttccgga aggccgccga tatcatcaag
     2281 agtgatttca ggaagcggag tcgccttcga agtcccattc ttcatcgaag aaacgcctac
     2341 cccgagcttc cctttcttat gaagaatcat taaagtcaaa aatgatgcta taatcaaacc
     2401 tccgacaaac aagccattat attggcctgc atcggcaaat gtatatgaaa ggttatattg
     2461 ttcaactaat tgttctgcca gttggctttg cggacggact tgcgttacgt acagtccatc
     2521 attcgtcttt agtgacaggg tgccgtcagc cttctccttc aaggatagag gctttccttc
     2581 ttgagcctgg atcgtcttct caaattccga aaacgacaca ttcattcctt tatttgcacc
     2641 cgagacgacg taccatccga cacttgctgc tattccaatg accaaaacga ctaggacgac
     2701 aatcttcgtt gcaaattttg aattcggctt caagccatcc ccaactccct taagaaagaa
     2761 tactattatt atataggaaa aagtgtcgac tgaacaagaa gaaagatgta gaaataatga
     2821 caat
//
LOCUS       DASULI010000296         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_7981, whole
            genome shotgun sequence.
ACCESSION   DASULI010000296 DASULI010000000
VERSION     DASULI010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7981"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1446
                     /locus_tag="VFX34_06265"
     CDS             262..1446
                     /locus_tag="VFX34_06265"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5564544.1"
                     /translation="MVAKQTIKDIELDGKRVFCRVDFNVPMENGEVADDTRIRAAVPT
                     MKFLADQGAKVILASHLGRPKGEVNEEMRLTAAGKRLSELLGKDVNKLDSSIGEEVEQ
                     AVADMQAGDIVLLENVRFHAGEEKNDPELAKKFADLADVFVNDAFGAAHRAHASTAGI
                     AQYIPAVSGFLLEKELEVLGKALSNPERPFTAIIGGAKVKDKIGVIDNLLDNVDHLLI
                     GGGLSYTFTRAEGYETGNSLVEEDKIELAKSFIQKAKEKNVKLHLPIDAVVADAFSEN
                     ANVKTVKIDEIPEGWMGLDIGPETAELYADIIKNSKLVIWNGPMGVFEMAPFAAGTEK
                     IAHAMAETEAYTVIGGGDSAAAVEKFDVAEKMDHVSTGGGASLEFMEGKELPGVTALN
                     DK"
     gene            1461..2222
                     /gene="tpiA"
                     /locus_tag="VFX34_06270"
     CDS             1461..2222
                     /gene="tpiA"
                     /locus_tag="VFX34_06270"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX5564545.1"
                     /translation="MRKRIIAGNWKMYKTMGEAKNFVAELKDRLPETDKVDAVICPPS
                     LHLSELVKLTEGTPLKIGAQTMHDADEGAFTGEVSPKMLADLNVPFVILGHSERRQYF
                     NETDEAVNRKTLAAFSKGMTPIVCVGESLEEREAGKTVETVAGQVIKAFEGVNADQAK
                     EAIIAYEPIWAIGTGKTATAQDANEVCGAIRAEIGKLYDDETASAIRIQYGGSVKPGN
                     IGELLSMEHIDGALVGGASLEPESFLEMIGVAANE"
     gene            2215..>2621
                     /locus_tag="VFX34_06275"
     CDS             2215..>2621
                     /locus_tag="VFX34_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279529.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5564546.1"
                     /translation="MSKAPVALIILDGFGLREERFGNAVAQANTPNFDILWNEYPHST
                     LTACGEAVGLPEGQMGNSEVGHLNIGAGRIVYQSLTRINKSIKDGDFMTNPALLGAIE
                     HVNKQDSSLHLMGLLSDGGVHSHYEHLFALLRLA"
BASE COUNT          722 a          588 c          761 g          550 t
ORIGIN      
        1 cttcgctgta cggcaaaggg cgccgtttcg cgaatccgcc ttatgcctgt cgcagctaaa
       61 gctgtcgcga aggacagctt ttgcgagtaa aagcgcagcg ttacgagcag taatagcgag
      121 cgcccttcgc ttttgtatta gccattctca tggcgaccca ttataataga tagatgggta
      181 ggaggaacgg tgaaacccgt tccttttttt tacattcctc gccgtttagg cgctcaactt
      241 tcttggaagg gtgcggttta cttggtagcg aaacaaacaa tcaaagatat tgaattggac
      301 gggaagcgcg tattttgccg tgtcgatttc aatgtaccga tggaaaacgg tgaagtggcg
      361 gatgatacga gaattcgtgc agctgttcca acaatgaaat tcttggcgga tcaaggcgca
      421 aaggtcatcc tcgccagtca tcttggacgt ccaaaaggtg aagtgaacga ggaaatgcga
      481 ctcaccgcgg ctgggaaaag attatcggag ctccttggaa aagacgtgaa caagctggat
      541 tcttccatcg gagaagaagt ggaacaagcc gttgccgaca tgcaggctgg cgacatcgtc
      601 cttctggaaa acgtcagatt ccatgccggg gaagaaaaga atgatccgga gctggcgaaa
      661 aagttcgcgg atctcgcaga tgtattcgtc aacgatgcat tcggcgcggc tcaccgtgca
      721 cacgcttcca cagcgggtat cgcgcagtac attccggcag tgagcggatt cttgcttgag
      781 aaggaattgg aagtcctcgg gaaagcgttg tcaaacccgg agcggccgtt cacagcgatt
      841 atcggcggcg caaaggtgaa ggacaagatc ggcgtcatcg acaatctgtt ggacaatgtg
      901 gaccacctat tgatcggcgg cggactttcg tatacgttca caagggctga agggtatgaa
      961 acaggcaact ctcttgtcga agaagacaaa atcgagcttg cgaaatcatt cattcaaaaa
     1021 gcgaaagaga agaacgtcaa actccatttg ccgatcgatg cggttgtggc ggatgcgttc
     1081 tctgaaaatg cgaatgtgaa aacagtgaag atcgatgaaa tcccggaagg atggatggga
     1141 ctcgatatcg gaccggaaac ggctgaattg tatgcggaca tcatcaagaa ttccaagctt
     1201 gtcatctgga acgggccgat gggtgtattc gaaatggcac cgttcgctgc aggtacggaa
     1261 aagatcgcgc atgcgatggc tgaaacggaa gcgtatacag tgatcggcgg aggcgattcc
     1321 gctgcggcag tcgagaaatt cgacgttgcg gagaaaatgg atcatgtatc aacgggcggc
     1381 ggtgcgtcat tggaattcat ggaagggaaa gaattgcctg gcgtaacggc gctgaatgat
     1441 aagtgacgga ggaattaaaa atgcggaaac gaatcattgc aggaaactgg aaaatgtata
     1501 agacgatggg ggaagcgaaa aactttgtgg cagaactgaa agatcgcttg ccggaaacgg
     1561 acaaggtcga cgcggtcatt tgtccacctt cccttcattt gtcagagctt gtaaaattga
     1621 cagaaggtac accattgaaa attggagccc aaacgatgca tgatgccgat gaaggagcat
     1681 tcacggggga agtaagcccg aaaatgctgg cggacctgaa cgtgccattc gtcatcctcg
     1741 gccattccga acgacgccaa tatttcaatg aaacggacga agcggtgaac cgcaaaacat
     1801 tggcggcgtt ttcaaaaggg atgacgccaa tcgtctgtgt aggtgaaagc cttgaagaac
     1861 gcgaagccgg gaaaacggtt gagacagttg caggccaagt gataaaagcg tttgaaggtg
     1921 tcaatgctga tcaggcgaaa gaagcgatca tcgcgtacga gccgatctgg gcaatcggaa
     1981 caggcaaaac ggcgactgca caagacgcga atgaagtgtg cggggcgatc cgtgctgaaa
     2041 tcggcaagct atacgatgac gagacagcat cagcgatccg catccaatat ggcggcagcg
     2101 tgaaacctgg caatattggc gagctattgt caatggaaca tatcgatggc gcacttgtcg
     2161 gaggagcgag ccttgagccg gaatcattcc ttgaaatgat cggagttgcg gcgaatgagt
     2221 aaagcgcctg tcgcactgat catcctcgat ggcttcgggc tgcgggagga acggttcggc
     2281 aatgcggtcg cacaagcgaa cacaccgaat ttcgacattc tgtggaatga atatccgcat
     2341 tccacattga ccgcttgcgg agaagctgtc ggccttccgg aagggcagat ggggaattcc
     2401 gaagtcggcc atctgaatat cggcgccggc cggatcgtct atcaaagcct gacacggatc
     2461 aacaagtcca ttaaagacgg agacttcatg acgaacccgg cgctgctcgg agcgattgaa
     2521 cacgtcaaca aacaagattc atcccttcat ttgatggggc tgctttcgga cggcggcgta
     2581 catagccatt atgagcattt attcgccctc cttcgcctcg c
//
LOCUS       DASULI010000297         5836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_226016, whole
            genome shotgun sequence.
ACCESSION   DASULI010000297 DASULI010000000
VERSION     DASULI010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226016"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFX34_06280"
     CDS             <1..318
                     /locus_tag="VFX34_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5564547.1"
                     /translation="PILDFQQILYGNSAKDDPNARVVVVQTEDLYIGLLVLDAKEILD
                     IPESKLTSSGLMAYSKTPYFKTVANLEDRMITVVDAEILSRTLTGMDEIGEYVHTQRM
                     QEK"
     gene            378..1568
                     /locus_tag="VFX34_06285"
     CDS             378..1568
                     /locus_tag="VFX34_06285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5564548.1"
                     /translation="MTTDRERQARVILHLDMNSFFASVEQAHDPNLKGIPMAVAGNPK
                     ERRGILVTCSYEARALGIYTTMPVGEAKRICPDLVIVPPDHEKYRIASNAVFTILRSY
                     TELVEPVSIDEAYIDITNIGGLTRASQIAEHMQQRILHELDLPCSIGIAPNKFLAKTA
                     SDMKKPMGITILRKRQVPDILWPLPVIEMHGIGKSTEKRLNEIGISTIGDLAKADESL
                     IKNTLGKNGTRLRNRANGIDFRPVDPDAAEERKSVGSSTTLPIDETELSACLEVFKQL
                     SEKVAQRLDRRQLAGNVIMIQIRTSDWRNQTRSRTVINPLYKAPDLYKEAASLFKQHW
                     DGEPIRLLGITVSNVVPRNELNEQLSFDSFEKHDREAKMDELVSELEKKFGPNSVKRG
                     LGER"
     gene            1578..2516
                     /gene="rnz"
                     /locus_tag="VFX34_06290"
     CDS             1578..2516
                     /gene="rnz"
                     /locus_tag="VFX34_06290"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX5564549.1"
                     /translation="MELFFLGTGAGMPSKQRNTSSLILNLSAEQMGYWMFDCGEATQH
                     QLLRTPIKPRKINKIFITHLHGDHIFGLPGFLGSRSFLGGDDELTIYGPVGIKEWITT
                     TLRLTKTHLTYPLKIEEIQGGCIFEDDWFKVSAKELEHVIECFGFRVEQKPLPGKLLA
                     DKAFEAGVPKGPLLKRLKDGEDIELADGRIVYSKDVTGDAQKGFTVAILGDTKYCSAA
                     IELADEADILIHEATFDNETGDLAKDYGHSTVGDAALTAKEANVKALIANHISARFLQ
                     GDITSLKEQGSAIFPNIHIAEDFSHFEWKNEQLIKK"
     gene            complement(2560..3579)
                     /gene="namA"
                     /locus_tag="VFX34_06295"
     CDS             complement(2560..3579)
                     /gene="namA"
                     /locus_tag="VFX34_06295"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HEX5564550.1"
                     /translation="MAKLFKPYTIRGMEMKNRIVMAPMCMYSSHNLDGKVEDWHKIHY
                     ATRAVGQVGLIIVEATAVLPEGRISDRDLGIWSDDHVEGLSEIVRLMKQHGAKTGIQL
                     AHAGRKATVDGDIYAPSAIRFNEQYKTPVEMSIAEIRETVDAFKQGAIRAKKAGFDCL
                     ELHAAHGYLINEFLSPLTNKREDEYGGPSENRYRILREIIDAVRSVWEGPLFVRISAE
                     DYTEGGMTTTQYIEMVNWMKSQDVDLIDVSSGAVVPAKIDAFPGYQVPFAEQIRKESG
                     IATGAVGLITTGLQAEEILMNGQADVVLLARELLRNPYWAYSAAKELGVSIEPPKQYD
                     RGWTF"
     gene            complement(3605..4414)
                     /gene="proC"
                     /locus_tag="VFX34_06300"
     CDS             complement(3605..4414)
                     /gene="proC"
                     /locus_tag="VFX34_06300"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5564551.1"
                     /translation="MGTIVFVGAGSMAEAIIAGILNNEVMRAKDVYVMNKSDDERLSY
                     MQETYGVSLVCQERNALREADLIVLATKPKDIKEAMHDISRFLNDNTAVLSVIAGVSI
                     ETIENGLGKRPIARSMPNTSATIGKSASGVSMNDLVDSTMRKQILDLLSSIGVVKEVQ
                     EDELHTVTALSGSGPAYIYFMVEALEEAAIAKGLSNEVARELIIQTFEGAAAMLRKTG
                     EEPERLRKNVTSPGGTTEAGLKALENRRFKETIQECINSAERRSRELGALS"
     gene            complement(4727..>5836)
                     /locus_tag="VFX34_06305"
     CDS             complement(4727..>5836)
                     /locus_tag="VFX34_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564552.1"
                     /translation="VDNEFVEDGIIEETEVNQCGSCGGNTVYDPVTRGLNCPFCGTEV
                     EIEATRENTTEHDYLEALDQADHSWSNEKRVFKCDNCGAETLLDNDKVADFCAFCGSS
                     HIAVTEHDAGIKPALVLPFQISKEQAVEKFKKWISKRYFAPSRLNKDYELKKISGAYI
                     PYWTFDSHTKSAYTVRIGTYYYVMETRTVHRDGKTEQVTEQVRKIRWRTEYGRYNKFF
                     DDVLVKASRNVASGLIDKVEPFQLDGLLDYRTEYLSGFLAERYSIPLKEGWFSARGII
                     DQRIRNGILRKETGDEVQIVSVDTSYDGITFKHILLPLWISSFAFNNKIYRFLVNGQT
                     GKVSGNAPISLWKVMFTILMIALVAGIVYLFQDVL"
BASE COUNT         1618 a         1376 c         1278 g         1564 t
ORIGIN      
        1 ccgattttgg attttcagca gatcctttac ggcaattctg ccaaggatga cccgaatgca
       61 agggtcgttg tcgtgcaaac cgaagattta tacatcggct tattagttct ggatgcaaag
      121 gaaattctgg atattccgga aagcaaattg acatcttccg gtctaatggc ttattctaaa
      181 acaccttatt tcaaaacagt cgccaacctt gaagatcgga tgatcacggt tgtggatgcc
      241 gaaattctat caaggacttt gactggcatg gatgaaatcg gagaatatgt gcatacacag
      301 cgaatgcagg aaaaatgaaa gagaaccgga tgggtccatc catccggttt tttaatgatg
      361 caatggagga gaggcaaatg actaccgacc gggaacgaca ggcaagagtc atccttcatc
      421 ttgacatgaa tagttttttc gcttccgttg agcaggccca tgatccaaat ctgaaaggca
      481 taccgatggc tgtcgctgga aatcctaaag aacggagggg catactcgtc acctgttcat
      541 acgaggcaag ggcgcttggc atatatacga cgatgccggt aggtgaggcg aaacgaattt
      601 gtccggacct tgtaatcgtg ccgcctgacc atgaaaaata tcggatcgct tcaaatgctg
      661 tcttcaccat tttaagatca tacacggaac tggtcgaacc tgtctcaatc gatgaagcgt
      721 atattgacat tacaaatatc ggcgggttga cgagagcaag tcaaattgcg gaacatatgc
      781 aacagcggat cctacatgaa ttggacctgc catgctccat cggaattgca ccgaataaat
      841 ttttagcaaa gaccgcttcg gatatgaaaa aaccgatggg cataacgatt ttgcgaaaaa
      901 ggcaagtgcc cgatattttg tggccgctgc ccgtcattga aatgcacgga atcggaaaaa
      961 gcacggaaaa acgattgaat gaaatcggta tttctacaat cggtgatttg gccaaagccg
     1021 atgaatcttt gattaagaac acattgggca aaaacggcac cagacttagg aaccgggcga
     1081 atgggattga tttcaggccg gtggatcctg atgcggcgga agaaaggaaa agcgttggaa
     1141 gctcgacgac attgccgatt gacgaaacgg aactttccgc ctgtcttgag gtgttcaagc
     1201 agttgtccga aaaggttgcc caaagattgg atagaagaca gcttgcagga aatgtcatca
     1261 tgatccagat tcgcacatcc gattggcgaa accagacgcg aagcaggaca gtcatcaatc
     1321 cgctatataa agccccggat ttgtataaag aagcagcatc tctttttaaa cagcattggg
     1381 atggagagcc aatcaggcta ttgggaatta cggtatcgaa tgtcgttccg agaaatgagt
     1441 tgaatgaaca attatcgttt gacagtttcg aaaagcatga cagagaagcg aaaatggatg
     1501 aactcgtttc agaacttgag aagaaattcg gtccgaattc agtaaaacgg ggacttgggg
     1561 aaaggtaggt gaatataatg gaattgtttt tcttaggcac aggtgccggc atgccatcca
     1621 agcaacgcaa cacatcgtcg ctgatcctca acttatccgc ggaacagatg ggctattgga
     1681 tgtttgattg cggagaggcg actcagcacc aactgctcag aacgcccatc aaaccccgta
     1741 aaatcaataa gattttcatc acacatttac atggcgacca tattttcgga ttgccgggct
     1801 tccttggatc gcgctcattt ttaggcggtg atgatgagtt gaccatttac ggtccagttg
     1861 gcataaagga atggatcacg acaacgcttc gtttaacgaa aacacacctg acatatcctt
     1921 tgaaaataga ggagattcaa ggaggctgca tctttgaaga cgactggttc aaggtatcag
     1981 caaaagaact tgagcatgtc atcgaatgtt tcggatttag agttgaacaa aagcctttgc
     2041 ctggcaagct tcttgcagat aaagcattcg aagcgggtgt gccaaaagga cctttgctta
     2101 aaaggttaaa agacggggaa gacattgagc ttgccgatgg gcgaatcgtt tacagcaaag
     2161 acgtaacggg cgatgcgcaa aaaggattta cggttgctat tctcggtgat accaaatact
     2221 gttcagctgc tattgaattg gcggatgaag cagacatcct aatccatgaa gctacttttg
     2281 ataacgaaac cggagacctt gcgaaagact acggtcattc aacggtaggc gatgcagcgc
     2341 taacagcgaa agaggcaaat gtgaaagcac taattgcaaa tcatataagc gcaagattct
     2401 tgcaggggga tattacgagt cttaaggaac aaggcagtgc tattttcccg aacatccaca
     2461 ttgctgaaga cttcagtcat tttgaatgga aaaatgaaca gttgataaag aaataagctg
     2521 ccgcagtcat tattgacaag cggcagcttt atatgttcat taaaaggtcc atcctcggtc
     2581 atattgctta ggcggttcga ttgaaacacc caattccttc gcagcagaat aagcccaata
     2641 tggattacgg agcaattccc ttgctaacag tacgacatcc gcctggccgt tcatcaatat
     2701 ttcctcggcc tgcaaaccgg tcgtgatcaa tccgactgca cctgttgcga taccggactc
     2761 ttttcgaatc tgctccgcaa aaggcacttg atagcccgga aacgcatcga tcttcgcagg
     2821 gacaaccgcg cctgaactga catcgatcag atcgacatcc tgtgatttca tccaattcac
     2881 catttcaatg tattgggtcg tcgtcattcc gccttctgta taatcctccg ctgaaatccg
     2941 cacgaacaat ggaccctccc atacagaacg gactgcatct ataatttcgc gcaaaatacg
     3001 atacctattt tccgaagggc ctccgtattc gtcttctctt ttgtttgtaa gcggcgaaag
     3061 gaattcattg atcaaataac catgggcggc atgcagctca agacaatcaa aacctgcttt
     3121 tttcgcccgg atggcacctt gcttgaaagc gtcaactgtc tcccggattt ctgcaatcga
     3181 catttcgacg ggcgttttat attgttcatt gaaacggata gcgcttggcg cataaatatc
     3241 gccatccacc gttgcctttc tgcctgcatg cgctagctgg atgccagtct tcgctccatg
     3301 ctgtttcatc aaacgaacga tttcagaaag accttctaca tggtcatcgc tccaaatacc
     3361 aaggtcccga tcagatatac ggccttccgg caatacagcg gtcgcctcca cgatgatcag
     3421 tccgacttgt ccgacagcac gtgtggcata atggatctta tgccaatcct caacttttcc
     3481 gtccaggtta tggctggaat acatgcacat tggtgccatg acgatcctgt ttttcatttc
     3541 cattccacga attgtataag gtttaaataa ctttgccaat acaataaccc ccttatccta
     3601 tccatcaaga caaagctccc agttctcggg aacgcctttc cgcactgttg atgcattctt
     3661 gaatcgtctc tttgaatcgt cgattctcta gtgccttcaa tccggcttcc gtcgtgccgc
     3721 cagggcttgt cacgttcttt cttaaccgtt caggttcttc accggttttg cgcaacattg
     3781 cggcagctcc ttcgaatgtc tggatgatca gctcgcgtgc gacttcattg gagagcccct
     3841 ttgcaattgc agcctcttcc aaagcctcta ccataaaata aatatatgcg ggaccactgc
     3901 cggataaagc cgtcactgta tgcagctcat cttcttgaac ctcttttaca accccgatgg
     3961 atgacaatag atccaaaatc tgtttcctca tcgttgaatc cactaaatca ttcatcgaaa
     4021 cgccgcttgc agatttaccg atcgttgcag aagtatttgg catggaacgg gcgatagggc
     4081 gcttgccaag accgttttca atcgtttcaa tcgaaacgcc tgcaatgacc gataatacag
     4141 ctgtattgtc attcagaaac ctgctgatat catgcatcgc ctccttgata tctttcggtt
     4201 ttgtcgccaa cacaatgagg tctgcctccc gtaaagcatt ccgctcttga caaaccagag
     4261 acaccccata cgtttcctgc atgtaggaaa gccgctcatc atccgattta ttcatcacat
     4321 aaacatcttt ggcccgcatg acttcattat tcagtatacc agcaataatc gcctctgcca
     4381 ttgaaccggc tcctacgaac acaatcgtac ccatcctcat ccatcctttc gtatgaataa
     4441 aaaaagcgct tccgtcccga taaaatcagg acgaaaacgc atgtttccgc ggtaccacct
     4501 aaatttgccg aaatcagtcc ggcacaactt actagctttc gtaacgtgaa agggacggcc
     4561 atgtttccat ggcagctcgg aagcaggttc ggggcaggag tgggatagcg gctttcagtc
     4621 aatggccgca tttcctgttt ccttcactgt ccgtactcgt cttcttcaat gcttacgtat
     4681 tgtaaattat agtgattatt gcacgtcgtc agataaaagt caatgattat aaaacatctt
     4741 ggaatagata aacaatgccg gcgacaaggg ctatcatgag aatggtgaac atcactttcc
     4801 acaaacttat cggggcattg ccacttactt ttccggtctg cccattcaca aggaaacggt
     4861 aaattttatt gttgaaggca aaggaggata tccatagcgg caataatata tgcttgaatg
     4921 ttatgccgtc ataggacgta tccacactca ctatttgcac ttcatcgccc gtttcctttc
     4981 gtaagatgcc attccgtatc cgttgatcga taatacctcg ggctgaaaac cagccctctt
     5041 ttaaagggat tgaataacgc tctgccagga agcccgatag gtattctgtt cgataatcta
     5101 gtaagccgtc aagttggaaa ggctcgactt tgtcaatcaa tccggaagcg acattccgtg
     5161 atgccttaac caatacatca tcaaaaaatt tattatatct accatattcg gtgcgccatc
     5221 ttattttcct gacttgttcc gtaacttgct ctgtctttcc gtcacgatgg acagtccttg
     5281 tctccatgac ataataatat gtgccaatcc tgactgtgta ggcggatttt gtatgggagt
     5341 cgaatgtcca ataggggatg tatgccccgc tgattttttt cagttcataa tccttgttta
     5401 atctggaagg tgcgaaatat cgtttggaaa tccatttttt gaatttctca acagcttgtt
     5461 ctttcgaaat ttgaaagggg agcaccaatg cgggtttgat acctgcatca tgctcagtga
     5521 ccgcgatgtg agatgagccg caaaatgcgc agaaatcggc caccttatca ttatccaaca
     5581 aggtttccgc accgcaatta tcacatttga atactcgttt ttcattgctc caactatggt
     5641 ccgcctgatc caatgcctct aaataatcat gttcagtcgt attttcacgg gttgcttcaa
     5701 tttccacttc agttccgcag aaagggcagt tcagtcctcg ggtaacagga tcatatactg
     5761 tatttcctcc gcaagaaccg cattgattta cttctgtttc ttcaatgatt ccatcttcaa
     5821 cgaattcatt atctac
//
LOCUS       DASULI010000298         7144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1860680,
            whole genome shotgun sequence.
ACCESSION   DASULI010000298 DASULI010000000
VERSION     DASULI010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860680"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1546)
                     /locus_tag="VFX34_06310"
     CDS             complement(68..1546)
                     /locus_tag="VFX34_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5564553.1"
                     /translation="MQLNNFIGGSWQGSGGANYAAVTNPANGEELAQVRLSSSEDVDL
                     AVKAAKEAQKKWALVPAPKRADYLYAIGNLMKERKEHLAQVLTKEMGKVIEEGRGEVQ
                     EGIDMAFYMAGEGRRLFGETVPSELQDKFAMSVRAPIGVVGLITPWNFPVAIATWKSF
                     PAIVAGNTFVWKPATETPMMAYEMAKIFEEVGLPAGVANIVFGSGSEVGTAMIEHPDV
                     RVISFTGSTETGRHVAETGGRHLKKVSLEMGGKNAVIVMDDADIDLAVEGILWSAFGT
                     AGQRCTACSRVIVHKDVKEELETKLLAAMEHLTIGDGLDESVKIGPVINGKALEKIQS
                     YVEIGKEEGAKLLAGGNVLTDGDFTKGHYFEPTLFTDVKWDSRLAQEEIFGPVVSLIE
                     VGSLDEAIEVNNSVIYGLSSSIFSKDVNKVFRAQRDLDTGIVYVNAGTTGAEIHLPFG
                     GTKGTGNGHRDSGVAALDVFTEWKSIYVDFSGKLQRAQIDTE"
     gene            complement(1681..2721)
                     /locus_tag="VFX34_06315"
     CDS             complement(1681..2721)
                     /locus_tag="VFX34_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HEX5564554.1"
                     /translation="MIGRNDPCPCGSGKKYKRCCASKAEISNEQLVDEELRRVISGYF
                     ENGISLPAEFAEYDRYERQWRSGLGRFFSSKEIKRVTGEYYLFIARRDLWRSHVLKEL
                     DGTVRSDSRRLLQHWNDPIVLLAKVKGFTEDTVTVEQILDKDTYILPLSPGNELKEGD
                     IFFGIALYDDRKPSTNAQLINGLYRVSDQSGSVQRKIVELAESSGFTNSFDFYNEHML
                     EVYKTLLSRASESDSEEMAVPDVETSLTEPQREVLEIAFAKLDEFHIEESMKNRLEPV
                     FEMFCLKEEPIIRKPEVLAASLFKTAHETGMLGDAQLTQTDAAKLFGVSVSAMMKNVD
                     TLTSYVTEVVQK"
     gene            complement(2770..4308)
                     /locus_tag="VFX34_06320"
     CDS             complement(2770..4308)
                     /locus_tag="VFX34_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX5564555.1"
                     /translation="MLEMDIKKMHEKPPYGMIAILFFGAFVAILNNTLLNIALPVIMN
                     EFAVKPSAVQWLTTGYMLVNGIMIPASAFFVQKFTNRRLFLTAMTLFTIGTTLAIFAP
                     SFGLLVAARMIQASGSALMMPLLMNVMLVAFPVEKRGSAMGMFGLVMFTAPAIGPTLS
                     GWIVEHYSWRTLFEIVLPFAILTIVFALWKLKNITPNTSVKLDVFSLALSSVGFGGLL
                     YGFSSAGDKGWGAPVVYGTIAIGAVALIVFILRQLRMGDPMLDFRIYKYPMFALASVI
                     SIVLSVAMFSGMILTPLYVQEVRGISPFEAGILMLPGAVLMGIMSPITGKLFDKYGAR
                     VLAITGLTITVITTFYLSRLELHSGYYYLMALYTVRMFGMSMVMMPIMTNGLNQLPMK
                     SNPHGTAMNNTLQQVSGAIGSAILLTIMTKRMEKSGESLFNDALASGNIPADKGGMAA
                     MEHAIGLKAMLEGINYSFLIATFISVAALILTLFVKRVRPPKYEGMKSPATDDPIKAK
                     TVRE"
     gene            4494..5378
                     /locus_tag="VFX34_06325"
     CDS             4494..5378
                     /locus_tag="VFX34_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5564556.1"
                     /translation="MNDRKLQVMLTAKKLFIEKGFTSTSIQDILEEAKISKGTFYNYF
                     TSKNECLMAILEDAHNESVIRRRELLVGRDKKDKDILAGQILVRMQVNRELNLLPIYE
                     AVFYSNDEELRNFVKAYHLSELKWIAGRLVDVYGEEAEPHAFDCSVMTLGMIQQLNHF
                     WKAGSSKDHDIEKLVHFTFRRMDALMSDMIEQKDVLLEKHVISSLETPAGDLKEKIQD
                     DLLELRKKLEQEQIGDCLEYTDFLLAELQTEKPRASLLEAVCRSFREAGNGTNLADSV
                     NEVLYPLWKYIDLIKSNS"
     gene            5438..6235
                     /locus_tag="VFX34_06330"
     CDS             5438..6235
                     /locus_tag="VFX34_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX5564557.1"
                     /translation="MNNHEIDFKLYGDDMQFVEVELDPGETVVAEAGSLMMMEDGIEM
                     ETIFGDGSGNSGGGFMGKLMGAGKRLLTGESLFMTTFTNEGSGKKHASFAAPYPGKII
                     PMDLSQYNGKIICQKDAFLAAAKGVSVGIEFQRKIGVGFFGGEGFIMQKLEGDGMAFV
                     HAGGTIIEKTLSPGETLRVDTGCLVAMTQDVDYNIEMVKGVKTALFGGEGLFFATLRG
                     PGKVWIQSLPFSRLASRVFAAAPQVPGGGSRDEGSIAGGLFNMLGGK"
     gene            complement(6428..>7144)
                     /locus_tag="VFX34_06335"
     CDS             complement(6428..>7144)
                     /locus_tag="VFX34_06335"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5564558.1"
                     /translation="TAGVVQPERKPNVDEGRAQMLAYEAYAADSEEERRELAGKAAAI
                     SPELPDALLLAAEVEEDAEKASSLFEKAIRNASKTFEPGENPWMNIPNRPFMRAAFAY
                     GVHLFKMREFDEAADVFKDLIRMNPTDNQGARYEAVACLIHAQRFNEAAEIMVRYEKG
                     SQSDAAYLYLDWKLEHEASEGKSEEAGELLEKAAKANGQVMHLMTFKAKTIDYPRQMN
                     IQPGGEDEARYIWLLLNGSN"
BASE COUNT         1836 a         1967 c         1563 g         1778 t
ORIGIN      
        1 agccagtaaa tgcccgattg gttcaactaa caatcagtgg aaaagctcaa ctgattgaag
       61 tttcacttta ttccgtgtcg atttgtgccc gttgcaattt cccgctgaag tcgacgtaaa
      121 tgctcttcca ctctgtgaat acatcaagcg ccgccactcc ggaatcccgg tgaccattgc
      181 ccgttccctt cgttcccccg aacggaagat ggatttccgc gccagtcgtt cctgcgttga
      241 cgtagacgat tcccgtatcg agatcccgtt gcgcccggaa cactttattc acatccttcg
      301 agaagatgga gctggacagg ccgtaaatga cgctattgtt cacttcaatc gcttcgtcca
      361 ggctgccaac ttcaatgagc gatacgaccg ggccgaaaat ttcctcctgc gccagacggc
      421 tatcccattt cacatccgtg aataacgtcg gttcgaagta atggcctttc gtaaagtcgc
      481 cgtctgtcag cacattcccc cctgccaata gcttcgcgcc ttcctctttt ccgatttcca
      541 catagctttg gatcttctcc aacgccttcc cgttgatgac cggcccgatt ttcacggact
      601 cgtccaagcc atccccgatc gtcaaatgct ccatcgctgc caataatttc gtttccaatt
      661 cttccttaac atctttatgc acgatgaccc ggctgcatgc tgtgcatcgt tggcccgctg
      721 ttccgaatgc gctccaaagg atcccttcca ctgccaagtc gatatccgcg tcatccatga
      781 cgatgaccgc atttttaccg cccatttcaa gcgacacttt cttcagatgg cgcccgcctg
      841 tttcagcgac atggcggccc gtttccgtcg atcccgtgaa tgaaatcaca cggacgtccg
      901 ggtgctcgat catcgcagta ccgacttcgg accccgagcc gaatacaata tttgcaaccc
      961 ccgcaggaag tccgacttcc tcgaaaatct tcgccatttc atacgccatc atcggtgttt
     1021 ccgtcgccgg tttccaaacg aatgtgttcc ccgcaacgat tgccgggaag gacttccatg
     1081 tcgcaatcgc aaccgggaag ttccatggcg tgatcaaacc gacaaccccg atcggagcgc
     1141 ggacgctcat cgcgaattta tcctgcagct cggaaggaac cgtttccccg aacagtctgc
     1201 gtccttcccc agccatatag aacgccatgt cgataccttc ttgcacttcc ccgcggccct
     1261 cttcgatgac tttccccatt tccttcgtca aaacttgagc caaatgctct ttacgttcct
     1321 tcatgagatt gccgattgca tataagtaat ccgcccgttt cggtgccgga acgagcgccc
     1381 attttttctg cgcttccttc gccgccttca cagccagatc gacgtcctcg cttgacgata
     1441 aacgcacttg cgccaactct tccccattcg ccggattcgt gacagccgca tagttcgctc
     1501 ccccgctgcc ctgccaagaa cctccgataa agttgttcaa ttgcatgatt ccatttcccc
     1561 tttccaacac gcactttttg agaccgctta caatctatct atttcgacat tatccttccg
     1621 aatcccttct tctatgaagg aaaaaaaatc ccgcacagga tatctcctgt acgggatttt
     1681 tcatttttga actacttccg tcacgtagga cgtcaatgta tcgacatttt tcatcattgc
     1741 cgatacggat acgccaaaca atttagctgc atccgtttgt gtaagctgag catctccgag
     1801 cattccggtt tcgtgggccg ttttgaagag ggaggccgca agaacttccg gcttccggat
     1861 gatcggctcc tcctttaggc aaaacatttc aaagacgggt tccaacctat ttttcatcga
     1921 ttcttcgatg tgaaattcat ccagtttcgc aaatgcaatt tcaagcactt cgcgttgagg
     1981 ttccgtcaga gacgtttcaa catccggcac tgccatctct tcagaatcag attcgctggc
     2041 gcgcgatagc aatgttttat aaacttccag catatgttca ttatagaaat caaagctgtt
     2101 cgtgaatccg ctcgattcgg ccaattccac gattttccgc tgcaccgaac cggactgatc
     2161 agacacacgg tataagccgt tgatcaactg ggcattcgtt gaaggtttcc gatcatcata
     2221 taaggcgatg ccgaaaaaga tatccccttc cttcaattca ttaccagggg ataatggaag
     2281 tatgtacgta tctttatcca aaatctgttc caccgtcacc gtatcttcag taaacccttt
     2341 cacttttgcc agcaaaacga tggggtcatt ccaatgctgc aacagccgac gggagtctga
     2401 ccgtactgtg ccatccaact ccttcagtac atggcttctc cataagtcgc gccgcgcaat
     2461 gaacaaataa tattcccctg tcacccgttt gatttccttg ctgctgaaaa atctgccaag
     2521 accggagcgc cattgccttt catatcggtc gtactcggcg aattcagcag gaaggctgat
     2581 tccgttttcg aaatagcccg agatgacccg cctcagctct tcatcaacca gttgctcatt
     2641 tgaaatctca gccttcgaag cacagcatct tttatatttc tttccgcttc cgcatgggca
     2701 gggatcattc cttccaatca ttccgctcac acctttcctc ttaattatac caaaagacgg
     2761 cgcaggggac tattcccgca ccgtcttcgc ttttatcgga tcatccgttg ccggtgattt
     2821 cattccttca tattttggag ggcggaccct tttgacaaag agcgtaagga tcaatgcagc
     2881 aacggaaatg aaggtggcaa tgaggaagga gtaattgatg ccctcaagca tcgccttcaa
     2941 gccgatcgca tgctccatcg ctgccattcc acctttatcc gcaggtatat ttccgcttgc
     3001 caatgcgtca ttgaaaaggg attcccccga tttctccatc cgcttcgtca tgatcgttag
     3061 cagaattgcg gatccgattg caccggacac ttgctgcaat gtattgttca tcgccgtacc
     3121 atgcgggttc gacttcatcg gcagctggtt caacccgttc gtcatgatcg gcatcatgac
     3181 catcgacatt ccgaacatcc ggactgtgta tagcgccatc aagtaataat aacctgaatg
     3241 cagttcaagg cggctcaagt aaaacgtcgt gatgactgtg atggtaagcc cggtgatcgc
     3301 aagaacccgc gctccgtact tatcgaacaa cttgcctgtg atcggcgaca tgatccccat
     3361 gagcactgct cccggaagca tcaagatccc cgcttcaaac ggcgaaatgc cgcgcacttc
     3421 ctgaacgtaa agcggcgtca ggatcatccc cgagaacatc gcaaccgaca aaacaatcga
     3481 aatgacggat gcaagagcaa acatcgggta tttataaatt cggaagtcga gcatcgggtc
     3541 ccccatccgc aattggcgaa ggatgaacac gatcagtgca actgctccga ttgcgatagt
     3601 tccataaacg accggagcgc cccatccttt atcacccgcc gaactgaatc catatagaag
     3661 accgccgaaa ccgacgcttg ataaagcgag ggagaagaca tccagcttca ccgatgtatt
     3721 cggcgtaata tttttcagtt tccaaagcgc aaagacaatc gtaagtatcg cgaacggcaa
     3781 cacgatttca aaaagcgttc gccatgaata atgctcgaca atccagcccg acaatgtcgg
     3841 cccgatcgcc ggcgccgtga acatgacaag cccgaacatc cccatcgccg aacccctttt
     3901 ctcaaccggg aaagcgacaa gcatgacatt catcaataac ggcatcatca aagcggagcc
     3961 cgatgcctgg atcatccgtg ccgcaacaag caaaccgaac gacggtgcaa agattgccag
     4021 agtcgttccg attgtaaata acgtcatcgc cgtcaggaat agcctgcggt tcgtgaactt
     4081 ctggacaaag aaagcacttg ccggaatcat gatcccgttc acaagcatat agccggtcgt
     4141 cagccattga acggccgaag gcttcaccgc aaactcattc atgatgaccg gcaaagcaat
     4201 attcaataat gtattgttca agattgcgac gaacgccccg aagaataaaa tcgctatcat
     4261 tccatatgga ggcttttcgt gcatcttctt tatatccatt tccaacatag ttcccccatt
     4321 caatcttaat ctcattaaca cattttcata ttttataccc ccagtccaaa ataatcaaat
     4381 atagaaaccg ttttcaatca aaaaaagttt ttaaccctga cgcatgcgca ttatacatgt
     4441 taaaatcaat ttggactcac agtacaaatt caaattaaaa cagggtgata gtaatgaatg
     4501 atcgtaaact gcaagtcatg ttaaccgcga aaaagctatt tattgaaaaa ggattcacat
     4561 ccacttccat acaagacatt ttagaggaag caaagatttc caaaggtacg ttttataatt
     4621 atttcacatc caaaaacgaa tgcctcatgg ccattttgga agatgcccat aacgagtcgg
     4681 tcatcagaag acgtgaactg ctcgtcgggc gcgataaaaa ggacaaagac atattagccg
     4741 ggcagatcct cgtccggatg caagtgaacc gggaactgaa tctgcttccg atttacgagg
     4801 cagtttttta ttccaatgat gaggaactcc ggaatttcgt gaaagcatac catctgagcg
     4861 agttgaaatg gattgccggc cgcctcgttg atgtctatgg cgaggaagcc gagccccatg
     4921 ccttcgattg ctccgtcatg acgctcggga tgatccaaca actgaaccat ttctggaaag
     4981 ccggctcatc taaagaccac gacatcgaaa agcttgtcca cttcacgttc cgaaggatgg
     5041 acgccctcat gtccgacatg attgaacaga aggacgtgct gcttgaaaaa catgtaatct
     5101 cgtctttgga aacgcctgca ggagatctga aggaaaaaat ccaggatgat cttctggagc
     5161 tgagaaaaaa attggaacag gaacagattg gggactgcct ggaatacaca gacttcctac
     5221 tggctgaatt gcagaccgaa aagcctagag cctccctctt ggaggctgtc tgccgttcat
     5281 tccgcgaagc gggcaacggc acgaaccttg cggacagtgt gaatgaagtg ctgtatccac
     5341 tatggaaata tattgacctg attaaatcaa attcgtgaaa ctttcagagc cgcctgccgt
     5401 acaattaatc agcaactaaa cttttaggag gcattttatg aacaaccacg aaatcgattt
     5461 caaactatac ggggacgata tgcaatttgt agaggtggaa ttggatccgg gcgaaacggt
     5521 cgtcgcggag gcgggttcat tgatgatgat ggaagacggg attgaaatgg agacgatctt
     5581 cggtgacggc tcgggcaatt ccggcggagg cttcatgggt aaattgatgg gagccgggaa
     5641 acgtctattg accggggaaa gtctattcat gacgacattc acgaatgagg gatccggcaa
     5701 aaagcacgct tccttcgcag cgccttatcc agggaaaatc atcccgatgg accttagcca
     5761 atataatggc aagatcatct gccagaaaga cgcattttta gcggcggcca aaggcgtttc
     5821 tgtcggaatc gaattccaaa ggaaaatcgg cgtcggcttt ttcggcggtg aaggcttcat
     5881 catgcaaaag ctcgaagggg acggcatggc attcgtgcac gccggaggaa caatcatcga
     5941 aaagaccctc tccccaggcg agacgttgcg cgtcgatacg ggatgcctcg tcgccatgac
     6001 ccaggatgtg gattacaata tcgaaatggt gaaaggagtc aaaacggcat tgttcggtgg
     6061 cgaaggattg ttcttcgcaa cgttgagagg cccggggaaa gtatggattc aatcattgcc
     6121 gttctcccga ctcgcaagcc gtgtcttcgc agcagcaccg caagttccag gaggcggctc
     6181 gcgtgacgaa ggcagcatcg caggtgggtt gttcaatatg ttgggcggaa aataaataag
     6241 taaaaccgga acgccgcggc gtcccggttt tttattgcgc gaaatacgca taaatttgta
     6301 tacacacgca taactgccgc tagacgcgca taaattcata aatcgacgca taaacgcttc
     6361 cagatgcgca taaatcgcca atttcgctca taaacgccaa aattcacgca taaacggacg
     6421 tattacctca attcgaacca ttcagaagca gccaaatata acgcgcttca tcctcgcccc
     6481 ccggttggat gttcatctgt ctcggatagt cgatcgtctt cgccttgaac gtcatcaaat
     6541 gcatgacctg tccatttgcc ttcgccgctt tttcaagcaa ctctccggct tcttcagact
     6601 tcccttcaga tgcctcatgt tccagtttcc aatcaagata taaataagca gcgtcacttt
     6661 gcgacccttt ttcataacgg accataattt cagcagcttc attgaaccgt tgcgcatgga
     6721 tcaagcaagc aaccgcttca taccgcgcgc cttgattatc cgtcgggttc atacggataa
     6781 gatctttgaa cacatccgcc gcttcatcaa actccctcat tttgaataaa tggacgccat
     6841 acgcgaaagc ggcacgcatg aacggccgat ttgggatgtt catccatgga ttttcgcctg
     6901 gctcgaacgt cttgctcgca ttgcggatcg ccttttcgaa taaggaggat gctttttccg
     6961 catcctcttc aacttccgcc gcaagcaata gtgcatccgg aagctcaggt gagatcgctg
     7021 ccgctttccc agcaagctct cgccgctcct cttcactatc tgccgcatac gcttcatacg
     7081 ccagcatttg cgctcggcct tcgtctacat tcggtttgcg ctccggctgc acaacaccag
     7141 ccgt
//
LOCUS       DASULI010000299         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_814220, whole
            genome shotgun sequence.
ACCESSION   DASULI010000299 DASULI010000000
VERSION     DASULI010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814220"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1024)
                     /locus_tag="VFX34_06340"
     CDS             complement(179..1024)
                     /locus_tag="VFX34_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5564559.1"
                     /translation="MDTYNFHDMKLAWLDGGVNFLDGGTMFGVVPKALWSRKYEVDEN
                     NLIELRTDPIYISYEGKHILIDSGIGKGKLSDKLKRNLGVRAETKVEESLAELGLTPE
                     DIDIVLMTHLHNDHAAGLTGWVDDELVSIFPNAEIHVSQVEWDEMRNPNIRSRNAYWK
                     ENWEPVQHQVKPFEGQVEILPGVEMIHTGGHSDGHSVIKLTRNGETILHMGDIMPTHA
                     HSNPLWVLAYDDYPMTSIYAKEKLMNEALPNGYRFIFYHDAVYRLVKWDESGKEITES
                     VRRTR"
     gene            complement(1048..1692)
                     /gene="trmB"
                     /locus_tag="VFX34_06345"
     CDS             complement(1048..1692)
                     /gene="trmB"
                     /locus_tag="VFX34_06345"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX5564560.1"
                     /translation="MRLRNKPWAKDFMAAHPDVLIHEDEGTAIDWKSVFGNENPIHVE
                     VGTGKGQFIIGMALANPDINYIGIEHFDNVIVSALEKVIEAEKPSNLRLLRGNGANLE
                     GLFQEGELDRIYLNFSDPWPKSRHAKRRLTHESFLARYEKVLKQKGEIHFKTDNRKLF
                     EYSLVSMSAYGMILQEVSLDLHAEMPEDNIMTEYEEKFSAKGQPIYRLEAMFTN"
     gene            complement(1711..2700)
                     /locus_tag="VFX34_06350"
     CDS             complement(1711..2700)
                     /locus_tag="VFX34_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX5564561.1"
                     /translation="MKLAQLVKLLDYVSRYENDLSRYPTQYIRLKNSQWKRMKTQWEH
                     GAYLSEGSHIPEEAEEQAEEGKWYSPLFRLFGNRKERLVEQEANETEEEGQDNADVLD
                     FNPNIIYNPKNEEQLRKLYLDQLFHFQLKWASSTMMEKSNLDAGFMRDSLLRSFTQQL
                     PDSYLLFYYPILKVKKAPVELDILLITPIECLCITVLEGEDAAAFIGEGDRFWTKKYG
                     DKESRVLNPLIGLNRMEKIVSGVFAAQELDFPIKKLVISRNGYIDYPGIPYDTDIIDR
                     KTYEEWFNTLRKSTVPMKYNQFKAAQSILDMGQTTAVSRLFEEQIEEQPETNE"
     gene            complement(2728..>3338)
                     /gene="dat"
                     /locus_tag="VFX34_06355"
     CDS             complement(2728..>3338)
                     /gene="dat"
                     /locus_tag="VFX34_06355"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEX5564562.1"
                     /translation="VKTGHIYLQVTRGASARVHQFPELAVTPVVTAYAIHNPRPLSNI
                     EGGVSVKSVEDVRWLRCDIKSLNLLANVLAKQEAYESGCAEAIFVRDGMVMEGSSSNA
                     FGVKDGVLVTHPATNLILNGITRQVVLKLCNEMGIPVEERAFTPDEMYAMDEFFITST
                     TAEITPVVLIDERPVGSGTPGEITRKLQEAFARQIPLSVARD"
BASE COUNT          834 a          851 c          692 g          961 t
ORIGIN      
        1 tgtaccgtgg aggcattaca agaatccgga atggcgaact ggagcaattt gaatttgcgg
       61 cggactcgca aacaggcaca gtcgttgaat tggtccaaaa ataaaagcgc agagcagcat
      121 aataaaagca acaggtgaag aaggaattct tcaacacctg ttgctttttt gtcactgctt
      181 atcttgtacg tcggaccgat tcggtgatct ctttgccact ttcatcccac ttgacgagac
      241 gatagaccgc atcatgataa aagatgaaac ggtatccgtt cggcaacgct tcattcataa
      301 gcttttcctt cgcatagatc gaagtcatcg ggtaatcatc ataagccaat acccataacg
      361 gattgctgtg cgcatgcgtc ggcatgatat cccccatatg aaggatcgtt tccccgttcc
      421 gggtcagctt gatgacacta tgtccgtcac tatggcctcc ggtatgaatc atttcaacac
      481 ccggaaggat ttcaacctgg ccttcgaatg gtttcacctg atgctggacg ggctcccaat
      541 tttctttcca atatgcattc cttgaacgga tattcggatt ccgcatctca tcccattcga
      601 cttgtgaaac gtggatttct gcatttggaa aaatcgacac aagttcatca tcaacccagc
      661 cagtcaatcc ggctgcatgg tcgttatgca aatgcgtcat aaggacaatg tcgatatctt
      721 cgggcgtcaa accaagctcg gctaagcttt cctccacctt cgtttctgca cggacgccaa
      781 gatttcgctt cagcttgtcc gaaagcttcc ctttcccaat tccggaatct atgaggatat
      841 gcttcccttc ataggaaatg taaatcggat cagtccgtag ctctatcaag ttattttcat
      901 cgacttcata ttttcgggac cataacgctt tcggtacaac tccgaacatt gtacctccat
      961 cgagaaaatt gactccccca tcaagccaag caagtttcat atcgtgaaaa ttatatgtat
     1021 ccaaacaaag cctccccttt cgaaaaatca attagtaaac attgcttcca aacggtaaat
     1081 cggctgccct ttagctgaaa acttctcttc atattccgtc atgatattgt cttcaggcat
     1141 ttctgcatgc aaatcgagcg acacttcctg aagaatcatg ccgtaagccg acatggaaac
     1201 gagcgagtac tcaaatagct tcctgttgtc cgtcttgaaa tggatttccc ctttttgttt
     1261 cagcaccttc tcatagcggg ccaaaaacga ttcgtgggtc aaccggcgtt ttgcatggcg
     1321 ggatttcggc catggatcgg agaaattcaa gtaaatccgg tccaattcgc cttcttggaa
     1381 taaaccctcc aaattggcgc catttcctct taagagacgt agattggaag gcttttcggc
     1441 ctcgatgacc ttttccaatg cggatacgat cacattatcg aaatgctcga tgccgatata
     1501 gttaatatcg gggttggcga gcgccattcc gatgatgaat tgcccttttc ctgttccgac
     1561 ctctacatgt atcggatttt cattcccgaa aaccgatttc caatctattg ctgttccttc
     1621 atcttcatgg atcaacacat caggatgcgc tgccatgaaa tcttttgccc acggtttatt
     1681 gcgaagccgc ataattcatt acactcattt ctattcatta gtttcaggtt gttcctcaat
     1741 ctgctcttca aacaacctgc tcactgcagt agtttgtccc atgtccaaaa tcgattgtgc
     1801 agctttgaat tggttgtact tcatgggtac agtcgattta cggagagtgt tgaaccactc
     1861 ttcatatgtt tttcgatcaa taatatccgt atcgtacggg attcccggat aatcgatata
     1921 gccattcctg gagatgacca gtttctttat ggggaagtcg agttcctgtg ccgcaaaaac
     1981 tcccgacacg attttctcca tccgattcaa accgatcaat ggattcaata cccttgattc
     2041 tttgtctcca tatttcttcg tccaaaaccg atcgccttca ccaatgaaag cggcggcgtc
     2101 ctccccttcc aacaccgtga tgcacaggca ttcaatcggc gtaatcagca ggatgtcgag
     2161 ttcaacgggt gcttttttca ctttcagaat cggataatag aacagaagat agctatccgg
     2221 aagttgctgg gtgaagcttc taagtaaaga atccctcata aaacccgcat ctagattgga
     2281 tttttccatc atcgtcgagc ttgcccattt cagttggaaa tggaatagct ggtccaaata
     2341 tagcttccgg agctgttctt cgtttttagg attgtaaatg atattcggat tgaagtccag
     2401 gacatcggcg ttgtcctgac cctcttcttc cgtttcgttc gcttcctgtt ctactagcct
     2461 ctctttgcga ttgccgaaca gacgaaaaag aggggagtac catttcccct cctccgcctg
     2521 ttcttccgcc tcttcaggga tgtgactccc ttcggaaagg taggcgccat gttcccactg
     2581 ggttttcatg cgtttccact gggagttttt caaacggata tattgtgtcg gataacgcga
     2641 taaatcattt tcgtatcgcg atacataatc aagcagtttg acgagctgcg ccaatttcat
     2701 ccactccgat cagtaagaca atatacatta atcccgcgct acagataacg gaatctgccg
     2761 cgcgaatgct tcttgcagtt ttctcgttat ctctccaggt gtaccggacc caaccggtct
     2821 ttcatcgatt aatacgacag gcgtgatttc agcggtagtg gacgtaatga agaactcatc
     2881 catcgcgtac atctcgtccg gcgtaaatgc tctttcctcg acaggtatgc ccatttcatt
     2941 gcaaagcttc aatacgactt ggcgtgtgat tccgttcaaa atgagatttg ttgccggatg
     3001 tgttacgaga acgccgtctt tcacaccgaa tgcattggat gatgaccctt ccatgaccat
     3061 tccgtcacgg acgaagatcg cctctgcaca gcctgactca tatgcttctt gtttcgccag
     3121 gacattggca agcaaattca aacttttaat atcacaacgg agccaacgca cgtcttcgac
     3181 cgatttcacc gagacgccgc cctcgatatt gctcaatggc ctcgggttat ggatggcata
     3241 cgcagtaacg acaggggtca ctgcaagttc agggaactga tggacccgcg ctgatgctcc
     3301 acgtgtcact tgcaaataaa tatggccggt tttcactt
//
LOCUS       DASULI010000300         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1587278,
            whole genome shotgun sequence.
ACCESSION   DASULI010000300 DASULI010000000
VERSION     DASULI010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587278"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="VFX34_06360"
     CDS             <1..153
                     /locus_tag="VFX34_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829253.1"
                     /note="similar to novel fructose-6-phosphate aldolase from
                     Escherichia coli; enzyme from Methanocaldococcus janaschii
                     shows transaldolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX5564563.1"
                     /translation="RHPQHVTEAALQGAHIGTMPLKVMKQLFNHPLTDKGIEAFLADW
                     EKRSAE"
     gene            174..1451
                     /locus_tag="VFX34_06365"
     CDS             174..1451
                     /locus_tag="VFX34_06365"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5564564.1"
                     /translation="MEVYKIRGGKRLHGTIKVSGAKNSAVALIPSAILADSPVTIEGL
                     PEISDVLTLKTLLEDIGGKVEFENGRMTVDPRGMIAMPMPNGNVKKLRASYYLMGAML
                     GRFKKAVIGLPGGCHLGPRPIDQHIKGFEALGAKVTNEHGAIYLRADELRGAKIYLDV
                     VSVGATINIMLAAVLAKGRTTIENAAKEPEIIDVATLLSNMGANIKGAGTNVIRIEGV
                     EKLHGTNHTIIPDRIEAGTFMIMAAAAGDGITIDNVIPFHVEALTAKLREMGVEVVED
                     EEKIFIPKPTNLQAVDVKTLVYPGFPTDIQQPFSVLLTQATGSSVITDTIYSSRFKHI
                     DELRRMNADARTEGRSAIISGPTELQAAAVRATDLRAGAALVLAGLIADGETEVHDIF
                     HVERGYSDLIEKLRGIGADIRKETVSSETVPKE"
     gene            1551..2516
                     /gene="glpX"
                     /locus_tag="VFX34_06370"
     CDS             1551..2516
                     /gene="glpX"
                     /locus_tag="VFX34_06370"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5564565.1"
                     /translation="MERSLSMELVRVTEAAAVSSARWMGRGLKNEADDAATTAMRTVF
                     DTIPMRGVVVIGEGEMDEAPMLYIGEQLGQGHGPEVDVAVDPLEGTNIVASGGWNALA
                     VLAIADRGNLLHAPDMYMNKIAVGPEAVGMVDIDAPVIDNLRAVAKAKNKDIEDVVAT
                     ILHRERHSKIIEEIRAAGARIKLINDGDVAGAINTAFDETGVDILFGSGGAPEGVIAA
                     VGLKCLGGEIQGRLVPSNNEELERCKKMGIDISRVLRMEDLVKGDDAIFAATGVTDGE
                     LLRGVQFKGAFSETHSLVMRAKSGTVRFIEGRHSLKKKPQLVMKN"
     gene            2703..>3892
                     /gene="rho"
                     /locus_tag="VFX34_06375"
     CDS             2703..>3892
                     /gene="rho"
                     /locus_tag="VFX34_06375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5564566.1"
                     /translation="MSKLTIAALEEMTLKELYTLARKYKISYYGKLTKKELIFAILKT
                     QAEQEGFFFMEGILEIIQSEGYGFLRPINYSPSSEDIYISASQIRRFDLRNGDKVTGK
                     VRPPKESERYYGLLHVEAVNGEDPEVARERVHFPALTPLYPDRHIKLETTPAKLSTRI
                     MDLVAPVGFGQRGLIVAPPKAGKTLLLKEIANAITENHPEAELIVLLIDERPEEVTDI
                     ERSVNADVVSSTFDELPENHVKVAELVLERAMRLVEHKRDVVILMDSITRLARAYNLV
                     IPPSGRTLSGGIDPAAFHRPKRFFGAARNLEEGGSLTILATALIDTGSRMDEVIYEEF
                     KGTGNMELHLDRSLAERRIFPALDIRRSGTRKEELLIKPDQLEKLWAIRKTFSDAPDF
                     AERF"
BASE COUNT         1088 a          827 c         1002 g          975 t
ORIGIN      
        1 cgtcatccac agcacgtaac ggaagctgca ttgcaaggtg cgcatattgg cacaatgccg
       61 ttaaaagtaa tgaagcaatt atttaaccat cctcttacgg ataaaggcat tgaagcattt
      121 ttggcagact gggaaaaacg atcagcagag taatggtcta aaaggagagc gacatggaag
      181 tttataaaat taggggcgga aaacgccttc acggcacaat taaagtgagc ggggctaaga
      241 atagtgcggt cgctctcatt ccatctgcaa tcttggcgga ttccccggtt acaatcgaag
      301 gacttccaga aatttcggat gtactgacat tgaagactct cctggaagac atcggtggga
      361 aagtcgaatt cgagaatgga agaatgacgg ttgatccgag gggcatgatt gcaatgccga
      421 tgcctaacgg gaatgtaaaa aaattgcgtg catcctatta cttgatgggt gcgatgcttg
      481 gccgctttaa gaaggctgtc attggtcttc cgggcggttg ccaccttggt cctcgcccga
      541 ttgatcaaca tattaaaggg ttcgaggcgc ttggcgcaaa agtgacgaat gaacatggcg
      601 cgatttactt gcgcgcggac gagcttcgtg gtgcaaaaat ttatcttgac gtcgttagcg
      661 ttggagcaac cattaacatc atgcttgcag cggtacttgc caaagggcgg acgacaattg
      721 aaaatgccgc aaaagaacct gaaatcatag atgtagcaac gcttttatct aatatgggtg
      781 ccaatattaa aggggcagga acgaatgtca tccgtattga aggcgttgaa aagctgcatg
      841 gaaccaacca tacgattatt ccggaccgaa tcgaagctgg cacgttcatg attatggctg
      901 cagctgccgg agatggaatt acaattgata acgtaatccc gtttcacgtt gaggctttga
      961 ctgcaaagct acgtgaaatg ggtgtcgaag ttgttgaaga tgaagaaaaa atatttattc
     1021 caaagccaac gaatctacag gcagtagatg taaagacgct cgtctaccca ggcttcccga
     1081 ctgacatcca acagcctttc tcagtcctgc ttacccaagc gacaggctct tccgttatca
     1141 cagatacgat ttattcctct cgttttaagc atattgatga attacgtaga atgaatgcgg
     1201 atgcccggac cgaagggaga tcggccatta tttctggacc gacggaattg caggcagctg
     1261 cagttcgggc aactgactta cgtgccggtg cggctctcgt cttagctgga ttaattgctg
     1321 acggcgaaac ggaagtgcat gatattttcc acgttgagcg tggatatagt gatttgattg
     1381 agaaacttcg aggcattgga gcggacattc ggaaggaaac ggtttcctct gaaactgttc
     1441 caaaagagta gaaaaggcat ggattttttg gcataatacc cgtccgtatt atgatagaat
     1501 atggaacgga gtaacacgga gttttttgtg aaacgggagg aaattataca atggaacgca
     1561 gcttatcaat ggaactagta cgggtaaccg aagcagcagc agtctcatct gcaagatgga
     1621 tgggccgcgg tctgaaaaac gaggcggacg acgcagcgac tactgcgatg cgtaccgttt
     1681 ttgatacaat cccgatgcgt ggtgttgtcg tcatcggtga gggagagatg gacgaagcgc
     1741 cgatgctata tatcggcgag cagcttgggc aaggacacgg accggaagtg gacgttgcgg
     1801 ttgacccatt ggaaggaaca aatattgtcg cttccggagg ctggaacgcg cttgccgttc
     1861 ttgcaatcgc ggatcgcgga aaccttctcc acgcaccaga tatgtatatg aacaaaattg
     1921 cggtcggacc tgaagcagtt ggcatggttg atattgatgc acccgtcatt gacaacctgc
     1981 gtgccgtagc aaaagcgaaa aacaaagata ttgaagacgt tgtggcaacg attctgcacc
     2041 gtgaacgcca ttcgaaaatc attgaagaaa ttagggctgc gggtgcgcgg attaaattga
     2101 ttaacgatgg tgatgttgcg ggagcaatca atactgcttt tgatgaaacg ggtgttgata
     2161 ttctatttgg ttccggtgga gctcctgaag gggtaattgc cgcagttgga ttgaaatgcc
     2221 tcggcggtga aatccaaggt cgacttgttc catccaacaa tgaggaattg gaacgatgca
     2281 agaaaatggg gattgatatt tcacgtgttc ttcgcatgga agacctcgtc aaaggtgacg
     2341 atgcgatttt tgcagcaaca ggtgttacgg atggtgaatt gcttcgcggt gttcaattta
     2401 aaggcgcatt cagcgaaaca cattcactag ttatgcgtgc taagtcagga acggtccgct
     2461 ttattgaagg ccgtcatagc ttgaagaaaa aacctcaact tgtcatgaaa aactgactga
     2521 cctctcgccc ggctttacgc cgggcaactt tccttgatag aatcccatca ttcttttgga
     2581 aattgccaac ctaagttcga gtgtagtctt ttcaaatctc ctgatccatt tgtaataaat
     2641 caaccgaatc ctgttttccc cttttaatca atgacaaata ataaaagagt ggtgccaact
     2701 atatgtcaaa gttaacaatt gcagcgcttg aagaaatgac gctgaaagag ctttatacgc
     2761 tcgcccggaa atacaaaatc tcatattatg gtaagttaac aaaaaaagaa ttgatttttg
     2821 ccatcttaaa aacacaagct gaacaagaag gcttcttctt tatggaagga attcttgaaa
     2881 tcattcagtc cgaaggctat ggttttttac gtccaatcaa ctattcaccg agctcagagg
     2941 acatctatat ttctgcttcc caaatccgta gatttgattt gcggaatggc gataaagtaa
     3001 caggaaaagt acgcccgccg aaagagtcgg aacgttacta tggcttattg catgtagaag
     3061 cggtgaacgg tgaagaccca gaggttgcgc gtgagcgtgt ccatttcccg gcactcacac
     3121 cactctatcc agatcgtcat attaaactgg aaacgacgcc agctaaatta tccacacgca
     3181 ttatggattt agttgcacca gtcggctttg gacaacgtgg gttgattgtt gcgccaccga
     3241 aagcaggtaa gacgctcttg ctaaaggaaa ttgccaatgc gatcaccgaa aatcatcctg
     3301 aggcagaatt gattgtcctc ttgattgatg agcgtccaga ggaagtaaca gacattgagc
     3361 gctctgtgaa cgctgatgtc gtcagctcca cattcgatga attgccggag aaccacgtaa
     3421 aagtggctga gctcgtattg gaacgggcta tgcgtcttgt cgagcacaag cgcgacgtcg
     3481 tcattctcat ggattcgatc acacggttag cgcgtgccta taacttggtt attccgccaa
     3541 gcggacggac gctttccggg gggattgacc cggctgcatt ccaccgtcca aaacgattct
     3601 ttggagccgc gcgtaatctt gaagaaggcg gcagcttaac gatattagcg acagccttga
     3661 ttgacacagg atcccgtatg gatgaagtga tctatgaaga gtttaaagga acagggaaca
     3721 tggagcttca tctggatcgt agcctggctg agcgccgtat tttccctgcg cttgatatcc
     3781 gccgctccgg cacgcgtaaa gaagagttgc ttattaaacc ggatcagctg gagaaacttt
     3841 gggctatccg aaagacattc tccgatgcgc ctgactttgc ggaacgcttc tt
//
LOCUS       DASULI010000301         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_241749, whole
            genome shotgun sequence.
ACCESSION   DASULI010000301 DASULI010000000
VERSION     DASULI010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241749"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFX34_06380"
     CDS             <1..665
                     /locus_tag="VFX34_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5564567.1"
                     /translation="FDVPQEKVRVICVEAAPMVLPGFDPELVDYAVRQLKAKGIEFSI
                     GTPVVEATPEGVNIKKGEDEFEFIKAGTVVWAAGVRGNRLIEQTGIENMRARVKVEKD
                     LRAPGHDDVFIVGDCALMINEETNRPYPPTAQIAMQQGDMCANNLIALIKGNPTSEFV
                     PDIKGAIASLGDDDAIGVAFGKKLMGKKASFMKKMVDNRSLYMIGGLGLTIKKGKFNL
                     LK"
     gene            738..1235
                     /locus_tag="VFX34_06385"
     CDS             738..1235
                     /locus_tag="VFX34_06385"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5564568.1"
                     /translation="MANNKRGNVWLGASGIVMNSNGEWLVVKKTYSGLKGMWSLPAGF
                     VEGNETADQAAIREVKEETGLDCELEGMVGFRTGILKGDISDNMAIFLLRPIREDQLL
                     IPQEQEISEVAWKTPFELKDDPNVSAMIHEIAERAIESGLLEMEQMDPGDWFGYTTYK
                     LFYKK"
     gene            1332..1658
                     /locus_tag="VFX34_06390"
     CDS             1332..1658
                     /locus_tag="VFX34_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuiB family protein"
                     /protein_id="HEX5564569.1"
                     /translation="MGTISLTHVVLSIIIFMVMFFGIGFLLNMLLRMTWLMAIVYPIV
                     VILIIDEVGFFEYFTKPGDSFGLLGEKIMSLHAADIIILASGLVGAIISGFVIKLLRK
                     QGYQMF"
     gene            complement(1708..2187)
                     /locus_tag="VFX34_06395"
     CDS             complement(1708..2187)
                     /locus_tag="VFX34_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEX5564570.1"
                     /translation="MSLLQNTPLLAALFAIFFAQFVKIPIHFLLTKKLDWKLMTSTGG
                     MPSSHSAAVSALTTAIAYEVGLGSPLFAVSAIFAVIVMFDASGIRYQAGQQALIINQM
                     RLDFQTFVTEVKGWQQKDGQQKIMELKTLLGHKRSEVFAGSMTGILISVITYSFIVV"
     gene            2340..>2699
                     /locus_tag="VFX34_06400"
     CDS             2340..>2699
                     /locus_tag="VFX34_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5564571.1"
                     /translation="MKTSFIEPDFGNLKSEVLIVGVPEHPENVEGWKTFADSFSPRLM
                     DWVKAGDVKTDFKKIIKMPSLDVRGYERIMFIGLGASKKLTEDRLRQAFAALGKELKS
                     SKATSVAIWTAPFTNEEL"
BASE COUNT          786 a          520 c          676 g          717 t
ORIGIN      
        1 agtttgacgt acctcaagag aaggtccgtg tcatttgcgt agaagcggcc cctatggttt
       61 taccaggatt cgatcctgaa ttggttgact atgcagttag acaattgaag gctaaaggaa
      121 ttgaattctc aatcggtacg ccggtagttg aagcgactcc tgaaggcgtg aacattaaaa
      181 aaggcgaaga tgaatttgaa ttcatcaaag caggcactgt cgtatgggca gctggtgttc
      241 gaggaaaccg tctgatcgaa cagactggca ttgaaaacat gcgtgcacgt gtcaaagtcg
      301 aaaaggatct tcgtgctcca ggacacgacg atgtattcat cgtaggtgac tgcgcattga
      361 tgatcaatga agaaacgaat cgtccatacc ctccgacagc gcaaatcgcg atgcaacaag
      421 gtgatatgtg tgcgaacaac ttgatcgcat tgatcaaagg caacccgact tccgaattcg
      481 ttccggatat taaaggtgcc atcgcatctc ttggtgacga cgatgcaatc ggtgtcgcgt
      541 tcggcaagaa attgatgggt aaaaaagcat cgttcatgaa aaagatggtt gataaccgtt
      601 ccctttacat gatcggtgga ttaggcctga cgattaaaaa aggtaagttc aacttactta
      661 aataataact gcaattatag cctattagac acccgcctat tctgtggcgg gtgtcttttt
      721 caagggggga taggtatatg gcaaataaca aacgcggaaa tgtatggctc ggcgcttccg
      781 gcatcgtcat gaacagcaac ggagaatggc tggtcgtcaa aaagacgtat agcggactga
      841 aaggaatgtg gtcgttgcct gcgggatttg tggaagggaa tgaaacagcc gaccaagccg
      901 caatccggga agtgaaagaa gagaccggcc tggattgtga actggaagga atggtcggtt
      961 tccgcacagg tatcctgaag ggtgatatta gcgataacat ggcaatattc ctattgcgtc
     1021 ccatccgtga agatcagctg ttaattcccc aagagcaaga gatttcggaa gtcgcttgga
     1081 aaacgccatt tgaattgaag gatgatccca atgtctccgc gatgatccat gagattgcgg
     1141 aaagagcgat cgaatcaggg cttctggaaa tggaacagat ggaccctggc gattggttcg
     1201 ggtatacaac gtataagcta ttttataaaa aatgatgttc gaattacgac aaatcattga
     1261 attaacgtca ttttattgaa agcatacatc cgttcctgta aaatgatgaa tggaaagcgg
     1321 aggtaaacaa attgggaaca atttctttga cacatgtcgt tttatccata attattttca
     1381 tggtcatgtt tttcggaata ggcttcttgc tgaatatgct gctccgtatg acgtggttga
     1441 tggcgatcgt ctatccgatt gtcgtcattt tgattattga tgaagtggga ttttttgagt
     1501 atttcacaaa accgggcgat tcattcggat tgcttggcga aaaaatcatg tcccttcatg
     1561 cagccgatat tatcattcta gcaagcgggc tcgttggcgc cattatttca gggtttgtca
     1621 ttaagctgtt aaggaaacaa gggtatcaga tgttctaatg taatcaaaaa agaaggtgtc
     1681 ccaactaggg agcaccttct ttttgattta cacaacaata aatgaataag taatgacgga
     1741 gatgaggatg ccggtcattg aacctgcaaa aacttcgctt cgtttatggc ctagaagggt
     1801 tttcagctcc atgatctttt gctgcccgtc tttttgttgc cagcctttca cttctgtcac
     1861 gaaggtttga aaatccagcc gcatttggtt gatgatcaat gcctgctggc ctgcctgata
     1921 ccggattccg gacgcatcga acatgacgat gaccgcaaaa atggcagata ccgcgaataa
     1981 aggggaacct aggccgactt catacgcgat ggctgtcgtc agtgcagaaa ctgccgccga
     2041 atgggagctc ggcatgccgc cggtggacgt catcaatttc caatccaatt tcttcgtcaa
     2101 taaaaagtgg atcggaattt taacgaattg agcgaaaaag atcgcaaata atgccgcgag
     2161 gagcggagtg ttttgcaata gggacatgat tccactcctt ttctaatgaa aaatatatag
     2221 atagtatacc acatcgattg tcagtgaata ttgaaagtcc gattcttttg aatgattctt
     2281 ttggttcgac atggtattgg tatataataa aggatgtatg aaaagtggga ggtagtcaaa
     2341 tgaaaacatc atttattgag cctgatttcg gtaatttgaa atcggaagtg ttaattgtag
     2401 gggttcctga acacccggaa aatgtggaag gatggaaaac gttcgccgac tcattcagcc
     2461 cgcgtctgat ggattgggtc aaagccggtg atgttaaaac agatttcaag aaaatcatta
     2521 aaatgccctc attggatgtg cgcggttatg agcgtatcat gttcattggc cttggggcat
     2581 caaaaaagct gacggaagat cgtttgcgcc aagcattcgc ggcattgggc aaagaattga
     2641 aatcttccaa agcaacatca gtggccattt ggacggcacc gttcacaaac gaagaactg
//
LOCUS       DASULI010000302         6624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_890811, whole
            genome shotgun sequence.
ACCESSION   DASULI010000302 DASULI010000000
VERSION     DASULI010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890811"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFX34_06405"
     CDS             <1..198
                     /locus_tag="VFX34_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5564572.1"
                     /translation="VHTLGDAHIYLNHMEQVKEQLSRTPKELPTLKLNTEGKSIFELT
                     YEDISVEGYDPYPKIKAPIAV"
     gene            221..709
                     /locus_tag="VFX34_06410"
     CDS             221..709
                     /locus_tag="VFX34_06410"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX5564573.1"
                     /translation="MISLLVAYDLNRVIGIDNKMPWHIPEELQYFKQVTMGKAIVMGR
                     KTFESIGRALPGRLNIIVTRNKDYKAEGAEVFHDLQKAIERGRAYSDEVVIIGGAEIF
                     NLSMDIADRLYITIIRKEYEGDTFFPEHDNNWKLVSESADHYTEDGIPYSYLIFDKAK
                     NA"
     gene            750..1589
                     /locus_tag="VFX34_06415"
     CDS             750..1589
                     /locus_tag="VFX34_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5564574.1"
                     /translation="MRKIHIVTDSTADLIENYTDQYDIHVVPLSIQIDGNTYSDGIDL
                     QPETFIEKMKGSNELPKSSQPAAGVFKELYDRLGEDGSEIISIHMTGGMSGTVKSAEA
                     AAGMTDSKVTVIDSMFISHALTFQVVEAAKMAKDGRSVEEIVKRLADVRRNTTLFVVV
                     DMLDNLVKGGRIGKGKALIGSLLNIKPIATLKDGVYTPVGKARSHKQVVSQLFKSFKE
                     ETAGKIIRSVGISHANGMAMAEPLLKLMEESGLNDVKFSFTSPIISTHTGEGAIGFMY
                     YTD"
     gene            1636..2448
                     /locus_tag="VFX34_06420"
     CDS             1636..2448
                     /locus_tag="VFX34_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5564575.1"
                     /translation="MRRIFLLIVFSFVLTGCQENAIYETSSFSERKSLALFDYSVPDY
                     FIPEKVTVIGLGDSLTQGVGDELKRGGYFGRVALAMNDWKGVEEIDSKNLAKRGRRSD
                     QLIDQLENDDIQAEIKKADVIYMTIGGNDMMKIVKANLFNLTAKPFYVELGKFENRLD
                     EIFKMIRGLNGDAIIVVAGLYNPLSIVTAEANEFETVIDDWNEAIEIQTIMDGKACFV
                     PVVDLFVSNENMVYHSDFFHPNAKGYESMANRFIEKIDQCGLTELSDGKLDM"
     gene            2461..3036
                     /locus_tag="VFX34_06425"
     CDS             2461..3036
                     /locus_tag="VFX34_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEX5564576.1"
                     /translation="MNLWKIAFFVLAGIIASAIIALVIFLEGPGDSDPLPPLNQYTPR
                     GSVLSVKATRNDLEVLANNYIRKAMKGEKLPVLMEVKDDVRLHSELTAFGMTFPLMMH
                     FDPVVLEDGNLMLKQTSMEIADLELPPSAVLSVLRDSVKLPPWMIVRPKEEEIFIHLS
                     ELEIAGDLHVRAKSVDLANDHIELEVTIPID"
     gene            3174..3704
                     /gene="msrA"
                     /locus_tag="VFX34_06430"
     CDS             3174..3704
                     /gene="msrA"
                     /locus_tag="VFX34_06430"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX5564577.1"
                     /translation="MAIQIATFAGGCFWCMVKPFDRYDGVISVVSGYTGGDVENPSYE
                     LVCTNTTGHREAVQITFDDEVISYEELLSVFWRQIDPTDAGGQFFDRGESYRTAIFTH
                     STEQEMLAEKSKQELASSGKFDKPIHTDILPAKTFYPAEEGHQNYYMKNPAHYERYAV
                     GSGRERFKTEHWGDQS"
     gene            3701..4126
                     /gene="msrB"
                     /locus_tag="VFX34_06435"
     CDS             3701..4126
                     /gene="msrB"
                     /locus_tag="VFX34_06435"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5564578.1"
                     /translation="MKENLKKTLTDMQYYVTQENGTEPPFNNEFDSHFEEGIYVDIVS
                     GKPLFSSKDKYDAGCGWPSFTKPIQSEEVTEHFDASHGMRRTEVRSRTADSHLGHVFP
                     DGPGPAGLRYCINSAALRFIPAEKLEEEGYSDYSHLFKD"
     gene            4140..4358
                     /locus_tag="VFX34_06440"
     CDS             4140..4358
                     /locus_tag="VFX34_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010300863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEX5564579.1"
                     /translation="MDRSFYQFVLSYRGGKKDDAKAVFAEAVFNDHSFPKDEKGFDPL
                     SRYLEEKADPEMPSVIFDELYALYKERF"
     gene            4442..5143
                     /gene="deoD"
                     /locus_tag="VFX34_06445"
     CDS             4442..5143
                     /gene="deoD"
                     /locus_tag="VFX34_06445"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX5564580.1"
                     /translation="MSIHINAKKGDIADTILLPGDPLRAKYIAETFLEDVTQYNEVRN
                     MFGYTGTYKGKRISVQGTGMGVPSISIYINELMQEYDVQKLIRVGTCGAIQKDVHVRD
                     VILAQSATTNSPINRTFFNGVDYAPTANFDLLLKAYNAGLEKGLKLKVGNIYTEDFFY
                     NEHAEHEKLAQYGVLAVEMEAAALYTLAAKYGRQALAVLTVSDHILTGEVTTAEERQT
                     TFNDMIVVALEAAIQ"
     gene            5230..>6624
                     /locus_tag="VFX34_06450"
     CDS             5230..>6624
                     /locus_tag="VFX34_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5564581.1"
                     /translation="MDEKEKEKEYEKEKESGHVEETHYQPPAKRYIRMKPFSLVMLIF
                     GLVLATAAVTFFALTTGEDKVVEVVSPQNPTTERKEFKKLYEAYDEMKKTYYNEIDET
                     AVIDGAINGMIEALGDPFSDYLNEKEARQLTESISSSFEGIGAEIQELNGYINIVSPI
                     KNSPAERAGLLPNDLIIAVDGKSIQGMSSSEAVLLIRGKKGTTVTLTVRRGELGEPFD
                     VKIERDVIPIETVYAEMLENNIAHIHITSFSEHTYEELLAALDEMEAKGMEGLIMDVR
                     QNPGGMLNTAIDISDLFVEKDKNLFQYEGKGNNPEVYVASDGRKVKVPVTLLIDDGSA
                     SASEILAGALKESANVPLIGLKTYGKGTVQTPKDLPDGSNLKLTTAKWLTPDGNWIHE
                     KGIEPDIEVPYPSYAMLPFLDPSMEMKEGMVSPAIKTAEEMLSAVGFDPGEIDGLFDQ
                     TTENAVLELQKALSL"
BASE COUNT         2023 a         1294 c         1632 g         1675 t
ORIGIN      
        1 gtccatacac taggtgatgc gcatatttat ttgaatcata tggaacaggt gaaggaacaa
       61 ttatcaagaa cgccgaaaga actgcctacg ttaaaattga ataccgaagg taaatcgata
      121 tttgaattga cctatgaaga catttcagtt gaaggatacg atccttatcc gaaaataaaa
      181 gcgccgattg cagtataaac aaagaaaaga ggatggtaac atgatttctt tacttgtagc
      241 ctatgatttg aaccgtgtga tcggcatcga caacaaaatg ccttggcata tccctgaaga
      301 acttcaatat tttaaacaag tgacaatggg caaggcgatt gtgatgggaa gaaaaacctt
      361 tgaatcaatc ggccgagcgt tacccggccg attgaatatc atcgtcacac gcaacaaaga
      421 ttataaggcc gaaggggcag aagttttcca cgatctccag aaagcgattg aaagagggag
      481 agcgtattcg gatgaggttg taatcatcgg gggcgcggaa attttcaatc tttcgatgga
      541 tatcgcagat cgactttaca tcacaatcat tcggaaagag tatgaagggg atacattttt
      601 ccctgaacat gataacaact ggaagcttgt gtcggagtcg gctgatcatt atacggaaga
      661 tggcatcccg tattcttact tgatttttga taaggcaaaa aatgcctaat tgataactgc
      721 cggcaatata gtaaaggaag tgcttagatt tgaggaaaat tcatatcgtc acggactcga
      781 cagcggattt aattgaaaac tatacggacc aatatgatat tcatgttgtt ccgcttagca
      841 tccagattga cggcaatacg tattcagatg gaatcgatct acaaccggaa actttcattg
      901 aaaagatgaa aggaagcaat gagcttccga aaagttcaca accagctgca ggtgttttca
      961 aagaattata tgaccgccta ggagaagacg gaagcgaaat catatccatt catatgacag
     1021 gcggtatgag cggaactgta aaatcggcag aagctgccgc cggaatgact gactcgaaag
     1081 tgactgtcat tgactctatg tttatttccc atgctttaac gtttcaagtc gttgaagctg
     1141 ccaaaatggc gaaagacggg agaagtgtag aggagattgt aaagagattg gccgatgtgc
     1201 gcagaaatac cacgctattc gtagtagtgg atatgttgga caatctcgtg aaaggcggcc
     1261 gcatcggtaa agggaaagcg ctgattggat cattattgaa tatcaaaccg attgcaactt
     1321 tgaaagacgg cgtctacacg cctgtaggca aagcacgcag ccataagcag gtcgtatcgc
     1381 aactcttcaa atctttcaaa gaagagacgg ctggtaaaat aatccgatca gtcggcatct
     1441 cccatgcaaa tggcatggca atggcagagc ccctattaaa gctaatggaa gaatcgggtt
     1501 tgaatgatgt aaaattctct ttcacttctc cgatcatcag cactcatacg ggagaagggg
     1561 caatcggttt catgtattat accgactgat ctatcttctg gcggttcaat aactaaaaaa
     1621 aggggaactg tgtttatgag aagaattttt ttacttatcg tcttctcatt cgttttaaca
     1681 ggatgccagg aaaatgccat atatgagaca tcgtcttttt ccgaaagaaa aagtttggca
     1741 ctgtttgact atagcgtacc ggattatttc attcctgaaa aggtcactgt catcgggctt
     1801 ggggattcac ttacccaagg ggtgggagat gaactgaaaa ggggcggtta tttcgggcga
     1861 gttgcacttg caatgaatga ttggaagggc gtcgaagaaa tcgattcaaa gaacttggcg
     1921 aaaagaggaa ggcgaagcga ccaattgatc gaccagctgg aaaatgatga tatccaagca
     1981 gaaattaaaa aggcggatgt catttatatg acaatcggcg ggaatgacat gatgaaaatc
     2041 gtcaaggcaa atctcttcaa tttgacggcg aagcctttct atgttgaatt gggcaagttt
     2101 gaaaatagac ttgatgagat attcaaaatg attcgcggat tgaacgggga tgccatcatt
     2161 gtcgtagctg gcttatacaa tccattgtcc atcgttacgg ccgaagcgaa tgaatttgaa
     2221 acggttatcg acgattggaa tgaagcgatt gaaattcaga cgatcatgga tggcaaggct
     2281 tgtttcgttc ccgtcgtcga cttatttgtg agcaatgaaa acatggtata ccactccgat
     2341 ttttttcatc caaatgcaaa aggatatgaa tcgatggcaa atcgttttat cgaaaagatc
     2401 gatcaatgcg gtttgacgga attgtcggat ggaaaattgg atatgtagag gtgaccgttg
     2461 gtgaatcttt ggaaaatagc atttttcgtc cttgctggaa tcatagcttc ggcgattatt
     2521 gcattagtta tatttcttga aggtccgggg gattcagatc cgcttccccc gttgaatcaa
     2581 tacacaccta ggggcagtgt gttaagtgtc aaagcaaccc gcaatgattt ggaggtactt
     2641 gcaaataatt acatccggaa agcgatgaag ggagaaaagc ttcctgtgct gatggaagtg
     2701 aaggatgacg tcaggctcca ctctgaattg actgcattcg ggatgacatt tcctcttatg
     2761 atgcattttg atccagtcgt tctcgaggac gggaatttaa tgctgaaaca gacttcgatg
     2821 gaaattgcag atttggaact acctccttca gccgttttaa gtgtattgcg agattcagtg
     2881 aagctgccgc cgtggatgat tgtcaggccg aaagaggaag aaatattcat tcacttatca
     2941 gagcttgaaa tcgcgggcga tttacacgtg agggctaaat cggtcgatct tgccaatgat
     3001 catattgagc tggaagttac aataccaatt gactaaagtt cagcatcggg cgccagaaac
     3061 tgggttcata aggcgaatat gctgcgtggc aaagaacgcc acttcgcatc ttcgtcttat
     3121 gctcgtcgtt tctagtcggg cgccctgcgc attctgaata aggagggata ttcttggcaa
     3181 ttcaaatagc gacatttgca ggaggctgtt tttggtgcat ggtaaagcct ttcgatcgat
     3241 atgatggggt tatttccgtc gtgtcaggat atacgggagg agatgtcgaa aatccttctt
     3301 acgaactcgt ttgtacaaat actacggggc accgtgaagc tgttcaaatc acatttgatg
     3361 atgaagtcat ttcatatgag gagctgctgt cagttttttg gcgccaaatt gacccgacgg
     3421 atgccggcgg acagtttttc gatcgtggtg aatcataccg aacagcgatt ttcacacatt
     3481 ctacagaaca ggaaatgctt gcagagaaat ccaaacagga gctggcttcc tcggggaaat
     3541 tcgacaagcc gatccacacg gatattctgc cggcaaaaac cttctatccc gctgaagaag
     3601 gccatcaaaa ttattacatg aaaaatcctg cacattacga gcgttatgct gtcggatcgg
     3661 gacgggaacg tttcaaaact gaacattggg gtgaccaatc atgaaggaaa accttaaaaa
     3721 gacattgacg gacatgcagt actatgtgac acaggaaaac gggacagagc ctccattcaa
     3781 taatgaattt gacagccatt ttgaagaggg gatctatgta gatatcgtct caggcaaacc
     3841 gcttttcagc tccaaagaca aatatgacgc aggatgcgga tggccaagct tcactaaacc
     3901 gattcaaagt gaagaagtga cagagcattt cgatgcaagc catgggatgc gcagaaccga
     3961 agtgcgcagc agaacggcag actcccattt gggacacgtg tttcccgacg gccccggccc
     4021 ggccggtctc cgctattgca tcaactcggc agctctccga ttcatcccgg cggaaaagct
     4081 tgaagaagaa gggtatagcg attactccca tttatttaag gactgaaagg ggttttatga
     4141 tggatcgatc tttttaccaa tttgtcttgt catatcgggg aggaaagaaa gacgatgcga
     4201 aagccgtttt cgccgaagcc gtgtttaatg atcacagctt ccctaaagat gaaaaaggct
     4261 ttgaccctct atcccgctac ttagaggaaa aagcagatcc cgaaatgcca agcgtgattt
     4321 tcgacgagtt atatgcattg tacaaagagc gtttctaatc gttctacgtt atcatcttca
     4381 ttgcataaaa tgtggattaa ctgtatgatt aggaaagaaa tttgaaagtg aggcgcataa
     4441 attgagcata catattaatg cgaaaaaagg cgatatcgca gatacgattc tacttcctgg
     4501 agatccgttg cgcgcaaaat atatcgctga aacatttttg gaagatgtaa cccagtataa
     4561 cgaagtcagg aatatgttcg gatacacagg aacgtataaa gggaagcgca tatctgttca
     4621 aggaacaggc atgggtgttc cgtccatttc aatttacatt aatgagttga tgcaagaata
     4681 tgatgtacag aaactgattc gtgtgggaac ttgcggagct attcaaaagg acgtacatgt
     4741 acgcgacgtc attttagctc aaagtgcaac gacgaactct ccgatcaacc ggacgttttt
     4801 caacggtgtc gactatgcac ccacagctaa tttcgattta ttgctgaagg cgtataacgc
     4861 aggtttggaa aaaggtctga agttgaaagt gggtaacatt tatacggaag acttcttcta
     4921 caatgaacat gccgaacatg aaaaacttgc acaatacggt gtattggctg tcgaaatgga
     4981 agcagccgcg ttatacacat tggcagcgaa atacggaaga caggcattag ctgtattaac
     5041 cgtaagtgac catatcttaa cgggtgaagt cacaactgct gaggaacgcc agacaacatt
     5101 caatgatatg attgttgtgg cacttgaagc ggcaattcaa taatcatgca tcggaggacc
     5161 gcttgctgta tcaacggcgg tctttttttt gaatgaaccg atgaatcggg aaagaaagtg
     5221 gggaatggga tggacgagaa agagaaagag aaagagtacg agaaagagaa agagagcgga
     5281 catgttgaag aaacacacta tcagccgccc gctaaacgat atatacgtat gaaacctttc
     5341 tcgttggtca tgctcatatt cggcctcgtt cttgcgacag ctgcggtgac gttttttgcg
     5401 ttgacgacag gcgaggataa ggttgtggaa gtggtcagtc ctcaaaatcc gacgactgaa
     5461 cgcaaggaat tcaaaaagct atatgaagct tatgacgaaa tgaagaaaac ctattataac
     5521 gaaattgatg agactgcggt tatcgatggc gccatcaacg gaatgatcga agcacttggc
     5581 gatccttttt cggactactt gaatgaaaag gaagcccgtc aactaaccga aagcatttca
     5641 tcaagttttg aaggaatcgg tgcagaaatc caagagttga atgggtatat aaatatcgtt
     5701 tcaccgataa aaaattcacc tgcggaacgt gctggccttc tgccgaatga tttgatcatt
     5761 gccgtagacg gcaagagcat ccagggaatg tcctcttcag aagctgtcct tctgattcgg
     5821 ggcaaaaaag ggacaacggt cactcttact gtccgacgtg gcgaattggg agagccgttt
     5881 gacgtgaaaa tcgaacggga cgtcattccg attgaaacag tctatgccga aatgctcgaa
     5941 aacaacattg cccatatcca tattacaagc ttttccgagc atacgtatga agagttattg
     6001 gcggcactgg atgagatgga agcaaagggc atggaaggac ttatcatgga cgtccgtcaa
     6061 aatcctgggg ggatgttgaa cacggcaatc gacatttcag accttttcgt cgaaaaggat
     6121 aaaaacctct tccaatatga agggaaaggg aataatcctg aagtgtatgt cgcctcggac
     6181 ggcaggaaag tgaaagttcc agtcactctg cttatcgatg atggcagtgc ctccgcttct
     6241 gagattttgg cgggcgcttt aaaagaatct gcaaacgtgc ccctcatcgg tttgaagact
     6301 tacgggaaag gaactgtcca aacaccgaag gacttgccgg acggctctaa tttgaaactg
     6361 acaacggcaa aatggttgac gcctgacggc aactggatcc acgaaaaggg gatcgaaccc
     6421 gatattgaag ttccataccc gtcatacgcc atgctcccat tcctagatcc ttcaatggaa
     6481 atgaaagagg gaatggtatc acctgcaatc aaaactgcag aggagatgtt aagtgcagtc
     6541 ggatttgatc cgggtgaaat cgatggactg ttcgatcaaa caacggagaa tgctgtacta
     6601 gagcttcaga aggcattatc cttg
//
LOCUS       DASULI010000303         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1260836,
            whole genome shotgun sequence.
ACCESSION   DASULI010000303 DASULI010000000
VERSION     DASULI010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260836"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFX34_06455"
     CDS             complement(<1..343)
                     /locus_tag="VFX34_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5564582.1"
                     /translation="MSIDFSPDKPIYQQLIDRILGDIMRGTLAAGEKLPSVREYAVEV
                     GVNANTMQRVYKELEQMEITETRRGQGSFVTENQEKITVLRNEMKEQLVTTFLQNVAA
                     FGFTTDEIVKCL"
     gene            complement(346..999)
                     /locus_tag="VFX34_06460"
     CDS             complement(346..999)
                     /locus_tag="VFX34_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564583.1"
                     /translation="MKSWNGLLRKEWVLLRITFLVLIAFIIFIAASSFAPLAVGGKFD
                     LIEITSMFSFLHMYFGALLFVHSLNTDMKRPDIWLHSPASIGKLLGAKMLMALLLVGL
                     SNLVWSGIGVFTYFIGGFVGVIPEWPNLLKVLLTTVFAISASLPIWVIYKILALRVGW
                     LAAIVMLIILFIGSILWGIMETIWHGFGPFAGSTLYIIVSIVLFAGGAILLEKKVRY"
     gene            complement(996..1778)
                     /locus_tag="VFX34_06465"
     CDS             complement(996..1778)
                     /locus_tag="VFX34_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564584.1"
                     /translation="MKQWNGLLQKEWVQWRGQIIVLIFLLLAGLLILPYFAGLLAVGE
                     VHVFEITMIISFVAAGACILVPVIAFSMMFNKDMKKPDLWFHSTASTVKLVVVKMVMS
                     AGVGLAYLLVTVLVVAIRYALTPQPEDIFNELLFYGSLLVGGVFLASIQFMVYGFFFI
                     VIDQLLKPFLKGFSFVVTLILFIISARMYGEVTGSNFYEKFVRVGGFDLMSLKNPKID
                     LQYDYFTITNTVLYAGEIIFNVLFTVGLFCLAMALFEKRVKL"
     gene            2232..2600
                     /locus_tag="VFX34_06470"
     CDS             2232..2600
                     /locus_tag="VFX34_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5564585.1"
                     /translation="MTEFLTGIDHIQIAAPPQSEEKAREFYGNLLGMEEIPKPENLQA
                     RGGCWFKCGVQEVHIGVQADFMPAKKAHPGFTVHALEQLKSRLETAGYKISEEPPIAG
                     RARFFTHDPFGNRIEFLEFD"
     gene            complement(2597..>2988)
                     /locus_tag="VFX34_06475"
     CDS             complement(2597..>2988)
                     /locus_tag="VFX34_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564586.1"
                     /translation="EGLSMWDTFLGGGLIVSALKYSEMNSSDDLVHRAQRALRHYETE
                     LMDVQNAATESFNVNHNDFFTFTDIFFDNIFSDWAVHSRITDAKSKLNTVLHDVRRVQ
                     DQLRRKRDEAMEELNRLDQQEKDIIVS"
BASE COUNT          905 a          767 c          573 g          743 t
ORIGIN      
        1 gcagacactt tacaatttca tcggtcgtaa atccgaatgc cgccacgttt tgaaggaatg
       61 tcgtcacgag ctgctccttc atttcattgc gaagcaccgt tattttctcc tgattttccg
      121 tcacgaacga tccctgcccc cttctcgtct cagtgatctc catttgctcc aactccttgt
      181 atacgcgctg catcgtgttt gcattgacgc caacttccac tgcgtactcg cggacggacg
      241 gcaacttctc ccccgctgca agcgtgccgc gcataatatc ccctaaaatc cggtcgatga
      301 gctgctggta aatcggctta tcgggtgaaa aatcaatgct catcgtcagt acctcacttt
      361 cttttcaagc aggattgccc cgcctgcgaa taatacaatt gaaacgatga tgtatagagt
      421 tgaaccggcg aaaggtccga acccatgcca gatcgtctcc ataatgcccc ataggatcga
      481 tcctatgaaa agaatgataa gcatgacaat tgccgccaac cagccaaccc tcaatgctaa
      541 aatcttgtaa atcacccata tcggcaaact tgcagaaatc gcaaagactg tagtcaataa
      601 cactttcagc aaattgggcc attccggaat aactcccaca aatcctccaa tgaaataagt
      661 gaaaacccct atgccactcc aaaccaggtt agatagccca accaatagta aagccatcag
      721 catttttgcc cccaaaagct tcccgatcga agctggcgaa tgcagccata tatccggtcg
      781 cttcatgtcc gtattaagac tatgaacaaa tagtaacgca ccaaaataca tgtgcaaaaa
      841 cgagaacata ctcgtaatct ctataagatc aaatttgccc cccaccgcca agggagcaaa
      901 acttgatgcg gcaatgaaga taataaacgc gattaatact agaaaagtaa tccgcagaag
      961 cacccattct tttcttaata aaccgttcca gcttttcata gcttcaccct cttctcaaac
     1021 aaggccattg ccaaacaaaa cagaccgact gtaaagagta cgttgaaaat aatctcaccc
     1081 gcatacaaaa ctgtattcgt gatggtgaag taatcatatt ggagatcgat cttagggttc
     1141 ttaagggaca tcaaatcgaa accgccaacc cggacgaatt tctcataaaa gttagaacct
     1201 gtaacctctc cgtacatcct tgctgaaatg atgaataaaa ttaatgtaac aacaaaggaa
     1261 aatcctttaa gaaaaggctt caacaactgg tcgatgacga taaagaaaaa tccgtatacc
     1321 atgaactgaa tggaagcgag aaaaactccg ccgaccagca aacttccata aaataacaac
     1381 tcattgaaaa tgtcttccgg ttgaggtgtt aatgcatatc tgattgcaac gacaagtacc
     1441 gtaacaagca aatatgccaa gcccactccg gctgacatca ccattttcac caccacaagc
     1501 ttcactgtgg aagccgtaga atgaaaccac aaatcaggct ttttcatatc cttgttgaac
     1561 atcatcgaga acgcaatgac agggaccaat atgcatgcac ccgctgctac gaaactaatg
     1621 atcatcgtaa tttcaaatac gtgaacttct cctactgcaa gcaacccggc aaagtacggc
     1681 aatatcaaca atcccgctaa taataggaag atcaacacga tgatctgccc tctccactgc
     1741 acccattcct tctgcaataa gccattccac tgcttcacca tctacccctc ctgtaccggt
     1801 gtattagtta catagtacac taatctatac tggaatgtaa aggaaattaa ctaacttgat
     1861 tgaaatatag caaaatactt cttgtgtggg tgtatggagt tatcgatcaa tttccgagag
     1921 ttatcggtca tttcactgaa gttatcggtc aattgagtga ggttatcggt cacatccagg
     1981 aagttatcgg ttagtttcac atttatatcg tcacttcggg actaatgaac ctccccatta
     2041 aaagagcttt taatttgtca aaggcattta gcgcgaccaa aaaaggctgt caagatgatt
     2101 cccccgcgca acttatcgta aagtcagtca tctataagca acccttatac atatcgttcc
     2161 atttcccgtc atagcttctc ttgataaact cctctacact ggagataaca atacaaaagg
     2221 ggcgaatgat aatgactgaa tttctcactg gtatcgacca tatccaaatc gcggcaccac
     2281 cccaatcgga agagaaagca agggaatttt acggcaatct gctcgggatg gaggaaatcc
     2341 cgaaaccaga aaacttgcag gcgcgcggcg gctgctggtt caaatgcggc gtgcaggaag
     2401 tccatatcgg cgtccaagcc gatttcatgc ctgctaaaaa agcgcatccc ggcttcaccg
     2461 tacatgcgct tgaacaattg aaatcccgat tggaaacagc tgggtacaaa atttcagaag
     2521 aaccgccgat tgcaggacgt gcacgcttct tcacacacga tccgttcggc aatcgtatcg
     2581 agtttttgga gtttgattaa gacacaataa tatccttctc ctgctgatcc agccggttca
     2641 attcttccat cgcttcatcg cgcttccgcc gcagctggtc ctgtacgcgg cggacgtcat
     2701 ggagcacggt gttcaatttg cttttcgcat ccgtaatccg cgaatgcacc gcccagtcgg
     2761 agaaaatatt atcgaagaat atatccgtga atgtgaaaaa gtcgttatgg ttcacgttaa
     2821 atgattcagt agccgcattt tggacgtcca tcaattccgt ttcataatgt cggagggctc
     2881 gttgtgcacg atgaacaagg tcgtccgagc tattcatttc cgaatacttc agcgctgaaa
     2941 cgatgagccc gccgcctaaa aatgtatccc acatggatag cccttccg
//
LOCUS       DASULI010000304         3875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_203022, whole
            genome shotgun sequence.
ACCESSION   DASULI010000304 DASULI010000000
VERSION     DASULI010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203022"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..654
                     /locus_tag="VFX34_06480"
     CDS             55..654
                     /locus_tag="VFX34_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5564587.1"
                     /translation="MSDIRLHRVLPFSKRLISETVLPGETVVDATAGNGNDTLFLAEL
                     VGENGRVFAFDIQQAALEATANRLAELNDRAALVLDSHANVDRYVKGRIGGAMFNLGY
                     LPYSEDLSIITKPDSTIEAIDKLLGLLKKGGIITISVYDGHEGGVEERDALLAYVKSL
                     HQADVHVARYELLNQRKNPPFLIAIEKVRDFDEVVRVQE"
     gene            complement(691..1419)
                     /locus_tag="VFX34_06485"
     CDS             complement(691..1419)
                     /locus_tag="VFX34_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5564588.1"
                     /translation="MNIFIITGASKGIGFELMRQLKAGNTKVIGIARTNESQVNDMVI
                     ADLSQTDQLAGMLRTIVEDNFASATSFTLINNAGTVEPIGLIGTIDETKLSQALAVNL
                     TAPMILSNAFISALEKFKGEKRIVNISSGAGRNPYEGWGVYCTTKAGLDHFSRVVSLE
                     QEKAEYPVDILSIAPGIIDTGMQETIRASNANDFPLHDRFVDYKELGHLSTAEETAGK
                     LIAVMNRKDFSMLGPIADIRQIAE"
     gene            complement(1416..1580)
                     /locus_tag="VFX34_06490"
     CDS             complement(1416..1580)
                     /locus_tag="VFX34_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564589.1"
                     /translation="MEKRRLIIGVITIIAIALYIWIAYFEIPSKVEKGEDGLQEESWK
                     VEIIMKGEVN"
     gene            complement(1690..2274)
                     /locus_tag="VFX34_06495"
     CDS             complement(1690..2274)
                     /locus_tag="VFX34_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX5564590.1"
                     /translation="MKKHIVTIIFTVVLLFGSSTESFAASSTYKVKKGDTLYRIAAMH
                     KITVKQLKTWNGITSDVIHPNQNLKVVKADTNVKKQTTSKKVAKTPSRSDDDNVVKEI
                     TVSASAYTANCNGCSGITSTGINLKRNPDAKVIAVDPSIIPLGTKVYVEGYGYAIAGD
                     TGGAIKGNKIDVFFPTKSEAYKWGRKQVKIKILK"
     gene            2401..2916
                     /locus_tag="VFX34_06500"
     CDS             2401..2916
                     /locus_tag="VFX34_06500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564591.1"
                     /translation="MTEILVRMARRNEAKTLADLLSRCQWFTYEKLFSAVYIEQLIKK
                     YYNVERIEQEVTAIDKSWHGYIVAEMEGTIVGVIGGGMTSGTDGEIYVFYVAPEARGS
                     GIGSRLLNFYTKIQRYTYGADRQWVSVAKGNMYGIPFYEAKGFSFEREEISYGSAVED
                     KDLSLVYSRRI"
     gene            3031..3693
                     /locus_tag="VFX34_06505"
     CDS             3031..3693
                     /locus_tag="VFX34_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5564592.1"
                     /translation="MPIPSEHQRPTRVTAKESAFTQLQQWIIDGTLQPGEKLNDIELA
                     EALGVSRTPIRESLQLLEVQGFVKMYPGKATQVTEVDKESINDLLPPLAAMQALSTEL
                     AAPNLNADAIKELEVTNNRFAKAVQEEDYFTALKIDEDFHQIIVDQAANKYIHTIVAS
                     LQTHVRRLFFHNSIILTEKSIDEHTDIIRLLKEGNGEKAAAIMRENWLRAIEEFHSKK
                     TK"
     gene            complement(3711..3854)
                     /locus_tag="VFX34_06510"
     CDS             complement(3711..3854)
                     /locus_tag="VFX34_06510"
                     /inference="COORDINATES: protein motif:HMM:NF017347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEX5564593.1"
                     /translation="MVTFYNSVLPLYSEGLGWLLPVVIVMIVTSIIAKLFQPSMQYKK
                     HSN"
BASE COUNT         1086 a          825 c          863 g         1101 t
ORIGIN      
        1 ggaacgccgc ggcagcttcc aaggaaagtt ttacgcaaag gcggttgaac gtatatgagt
       61 gatattcgat tgcatcgggt tttgcctttt tctaaacggt tgatcagtga aactgttttg
      121 ccgggcgaaa cggtagttga cgcaactgca ggaaacggca acgacacgtt attcctagcg
      181 gagctcgtag gtgaaaatgg acgtgttttc gctttcgata tccagcaagc tgcgctcgag
      241 gcgactgcaa atagactggc cgaactgaat gaccgtgccg cgttggtatt ggacagccat
      301 gccaatgtgg ataggtacgt gaaggggagg attggcggag cgatgttcaa tctagggtat
      361 cttccgtata gtgaagactt atccatcatc acaaaaccgg attccacaat tgaagctatc
      421 gataagctgc ttggtctgct taaaaagggt ggaatcataa cgatttccgt ctatgatggc
      481 cacgaaggcg gggtagagga acgggatgca ttgctggcat atgtgaaatc gcttcatcag
      541 gcagatgttc acgtcgccag atatgaattg ctcaaccaac gaaaaaatcc cccatttttg
      601 attgcaatcg aaaaagtgag ggattttgat gaagtcgtgc gagtgcaaga ataagaaagc
      661 gcttggatcg ttttaggatc caagcgtttt tcattctgcg atctgccgaa tatccgctat
      721 cggtccgagc atgctgaagt ctttgcgatt cattacagct atcagtttgc cagctgtttc
      781 ttccgcagtg cttaaatgtc ctagttcctt gtagtcgacg aaacggtcgt gcaatgggaa
      841 atcgttggca ttgcttgccc ggattgtttc ctgcatgccg gtatcaatga ttccaggggc
      901 aatcgatagg atgtccacag ggtattccgc tttttcctgc tccaacgata cgacgcggga
      961 gaaatgatcg agccccgctt tcgtcgtgca ataaacgccc catccttcat atggattcct
     1021 gcctgctcct gaagaaatat tgacaatcct tttttcacct ttgaattttt ccaatgctga
     1081 tatgaatgca ttcgatagga tcatcggagc tgtcaaattg acagcaagcg cttgcgacag
     1141 tttcgtttcg tcaatcgtgc cgatcaaacc gatcggttcc accgttcctg cattattaat
     1201 caacgtaaag gaagttgcag aggcaaagtt gtcttccaca attgtgcgca gcattccagc
     1261 caattgatcg gtctgcgaaa gatccgcaat caccatgtca ttaacttggc tttcatttgt
     1321 cctagcgatg ccgatcacct tggtgttccc cgccttcaac tgcctcatta attcaaaacc
     1381 gatccctttg gatgctccgg taataataaa tatgttcaat tgacttcccc cttcataatg
     1441 atttccacct tccaagactc ctcttgaagc ccgtcttccc ctttttccac ctttgatgga
     1501 atttcaaaat aagcaatcca aatatataac gcgattgcaa taatcgtgat caccccgatg
     1561 atcaatcttc tcttctccat ctccactccc cattcccctc attttacatc agtttaccat
     1621 gatggatgga agtgtggaga aattaaaata gccaatccct tcggaagtga aaggattggc
     1681 cagttaaggt catttcaata tttttatctt cacttgtttt ctaccccatt tatacgcttc
     1741 gcttttagtt gggaagaaga catcgatttt atttcctttt atcgcgccgc cagtatctcc
     1801 tgcgatggca tatccatacc cctccacgta tactttcgtg cctaatggta taatggatgg
     1861 gtcaacagcg atcacttttg catccggatt gcgctttaaa ttgataccgg ttgaagtgat
     1921 tccggagcag ccgttgcagt tcgctgtata tgcgcttgca gaaaccgtga tttccttcac
     1981 tacattgtcg tcatcggatc gtgatggtgt tttggcaact tttttagatg tcgtttgttt
     2041 tttgacgttt gtatcagctt tgacaacctt caagttttga ttcggatgaa tgacgtctga
     2101 agtaatgcca ttccatgttt tcaattgttt cacagtgatc ttatgcattg ctgcgattct
     2161 gtagagcgtg tcaccttttt tgaccttata cgttgaagag gctgcaaaac tttcggtcga
     2221 gctaccgaac aataaaacta ctgtaaagat aatcgtcaca atatgttttt tcaagttttc
     2281 tactccccgc ctttttagtc tactcataga ttaacagtga agtattacac ggatgttaca
     2341 gtttgcaacc attatgatta caaaagaaaa atgattatta cattgggagg aagatgaaaa
     2401 ttgactgaaa ttttagtgag aatggcaagg aggaacgaag cgaaaaccct tgctgacttg
     2461 ctttccagat gccaatggtt cacatatgaa aagctatttt cagcagtcta tattgagcaa
     2521 ctaattaaga aatattacaa tgtcgaacgc attgaacagg aagtcacagc aatcgacaaa
     2581 agctggcatg gctatattgt cgccgaaatg gaaggaacta tcgttggagt cataggcgga
     2641 gggatgacaa gcgggaccga tggagagatt tacgtcttct acgtcgcacc ggaagcaaga
     2701 gggtctggga tcggttcgag actcctcaat ttttatacaa aaatccaaag gtatacatac
     2761 ggagcagatc ggcaatgggt ttcggttgca aaaggcaata tgtacggaat tcctttctat
     2821 gaagcgaaag gcttttcatt tgagcgtgaa gagatttcat atggatcggc tgtagaggat
     2881 aaagatctat cactcgtcta ttcccgacgg atataagacc gatatattgt atatcattcc
     2941 tttcctctta tatccttttc atgtatacta atagtactag aagtgaagta ggataggata
     3001 ttcaaattgc aagcaggatg gtgatcagca atgccgatac cttcggaaca tcaacgaccg
     3061 acacgtgtaa cagcgaaaga aagtgcattt actcaattgc agcaatggat tatcgatgga
     3121 acattgcagc caggtgaaaa gttgaatgat attgaattgg cggaagcact cggtgttagc
     3181 agaaccccga tccgcgaatc gcttcagttg ttggaagtac agggatttgt gaaaatgtat
     3241 ccgggaaaag ctacacaggt gacggaggtg gacaaagagt cgatcaatga cctgttgccc
     3301 cccctagctg ccatgcaagc attatccaca gagcttgcag ccccgaactt gaatgcagat
     3361 gccattaagg aattggaagt gacgaataat cgtttcgcta aggcggtcca ggaagaggat
     3421 tattttacgg cactaaaaat cgacgaggac tttcatcaaa tcattgtgga ccaagcagca
     3481 aacaagtata ttcatactat cgtagcatcg ttgcaaaccc atgtacgccg gcttttcttc
     3541 cataactcaa tcattttgac ggaaaagtcg attgatgagc atactgatat tatcaggttg
     3601 ctgaaagaag gcaatggtga aaaagcggcc gcgatcatgc gtgaaaactg gctccgtgca
     3661 attgaagaat ttcattctaa aaaaacaaaa taaaaaagca ctccaattta ttaattggag
     3721 tgctttttat attgcataga cggttggaac aacttggcga taatacttgt aactatcatt
     3781 acaatcacta ctggaagaag ccagccaagg ccttcactgt ataaaggcaa tacactattg
     3841 tagaaagtta caatcccgtt aagccaattc ggcca
//
LOCUS       DASULI010000305         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1582927,
            whole genome shotgun sequence.
ACCESSION   DASULI010000305 DASULI010000000
VERSION     DASULI010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582927"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFX34_06515"
     CDS             <1..606
                     /locus_tag="VFX34_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEX5564594.1"
                     /translation="MNIFYNHNGVGDVLLVQLTTERPENREIENKKDITLIKDEATAS
                     IVAFNVFNASTYVKVETDGQVETTEEIVGQLQEALKANGAELELDVDFTPKFIVGHVT
                     AKEKHPNADKLSVCTVDVGNETLQIVCGAPNVEEGQKVVVAKVGAVMPSGMVIRDAEL
                     RGIASSGMICSAKELGIPNAPTEKGIMVLAADATPGEAFII"
     gene            722..>3117
                     /locus_tag="VFX34_06520"
     CDS             722..>3117
                     /locus_tag="VFX34_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX5564595.1"
                     /translation="MTWIKKMMTRLFDADKNEEFEESFDDDIELEEYKEEPRAEKPAF
                     RFPIITDAEIYGWEPDSENEIKRADNLVTELDDDDGFEPLPLYKNDRWPGGNEKPTIH
                     RATGQKKYAEKKEMKTEHALNRKTVPIEEMEQVKKTIIQKRSNRPFTPTEVPSPIYGY
                     SRPKKERFEALQFEKAEAAAEEFAIPSLRESEYDQYEGKPTIKQSAAEDEIHRIELEG
                     HAHTSDKEMMDEKISDDPAEGNSKEQVEQDPEVELNSEEQQQMDSQRLIDVVGEVEAE
                     AFEILTDGAEEPIAPEVEEEFNEISIPTVEGEFEAINDAELTDKEESESASDPESLSE
                     VEEAKSEPADVPEQKKDRIVPFNVLMLKNDKLKYEQREKRAGSQSAVDATKNDFTRTE
                     QVKDIPIPEELDEEPEEQPYYAFPSKDFLIQPEEHIEDTEWLESQSKKLEEALSYFAV
                     QANVIEAVQGPTVTRFELTVGHGTKVSKVRNLTDDLKLALAAEDIRIQAPIPGTSSIG
                     IEIPNRKPRAVRISEVIETERFQGSGSPLEAVLGLSLTGEPVTLDLRKMPHGMIAGAT
                     GSGKSVCINSILISLLYKASPSDLKMLLIDPKMVELAPFNGIPHLLSPVITDVKAATA
                     ALKWAVGEMERRYELFAHVGARNIERYNEMAQKDRKFSLKLPYLLIVIDELADLMMMA
                     PADVEVSIGRITQKARACGIHLIIATQRPSVDVITGTIKANVPTRIAFSVSSQIDSRT
                     IIDTPGAERLLGRGDMLYLGNGQSSAVRLQGTFVTDAEIERIIEHVQNEAEPDYLFGQ
                     "
BASE COUNT          983 a          624 c          809 g          701 t
ORIGIN      
        1 atgaatattt tctacaatca caacggggtc ggagatgtac ttctcgttca attaacgact
       61 gaacgccctg aaaataggga aattgaaaac aagaaggata ttacacttat caaagacgag
      121 gcaacagcta gcatcgtagc attcaacgtc ttcaatgcat cgacttatgt gaaggtcgaa
      181 actgacggac aagttgaaac gacggaagaa atcgtaggtc agttgcagga ggcgcttaag
      241 gcgaacggcg ccgagcttga actggatgtg gatttcactc cgaagttcat cgttgggcat
      301 gtcacagcga aagagaagca tccaaatgcg gataagttga gcgtatgtac ggtcgatgtc
      361 gggaatgaaa cacttcaaat cgtctgcgga gcgccgaatg tcgaagaagg ccaaaaagtt
      421 gtcgtagcaa aagtaggggc agtcatgcca tccggaatgg tcatcagaga tgcggagctt
      481 agaggaatcg catcctcggg gatgatctgt tctgcgaaag aacttggcat cccgaatgct
      541 ccaacagaaa aagggattat ggtccttgcc gctgatgcga ctccaggtga agcttttata
      601 atttgatcat tgacaaacgt actgcccaaa gcagtacgtt tttcttattt tggcgatccg
      661 attgatttaa tctatgaaaa cctgctaaaa tagaatgatc gacttgaaaa gagtgataga
      721 tttgacttgg attaaaaaaa tgatgacccg tttatttgat gcagataaaa atgaagaatt
      781 tgaagaaagt tttgatgatg acattgagct tgaagaatat aaagaggaac cccgtgccga
      841 aaaaccggct ttcagatttc caatcatcac cgatgctgaa atctacgggt gggaacccga
      901 ttcggagaac gaaataaaaa gagcggataa tttggtaaca gagcttgatg atgatgatgg
      961 tttcgaacca ttacctttat ataaaaatga tagatggcct ggcggaaacg aaaaaccgac
     1021 aattcatcgg gcaactggtc agaagaagta cgctgaaaaa aaggaaatga aaacggagca
     1081 tgccctgaac cggaaaacgg tacccattga agaaatggag caggttaaga aaaccatcat
     1141 acaaaaacga agtaaccgtc ccttcactcc tacggaagtg ccgtcgccga tttatggtta
     1201 ttcaagaccg aaaaaggaac ggtttgaggc ccttcaattt gagaaggcgg aagcggcagc
     1261 tgaggaattt gcaatcccgt ccctaagaga atcagaatat gatcaatatg aaggtaaacc
     1321 gacgattaaa caatcagcag cggaagatga aattcatcgc attgaattgg aaggtcatgc
     1381 acatacttcg gataaagaaa tgatggacga gaagataagt gatgatccgg ccgaagggaa
     1441 ttccaaggaa caggtagagc aagatcccga agtggaattg aatagtgaag agcagcaaca
     1501 aatggacagt caacgattga ttgatgtcgt tggggaagtt gaggctgaag ctttcgaaat
     1561 actgactgac ggagctgagg aaccgattgc tcccgaagtt gaagaggagt tcaatgaaat
     1621 ctccattccg acagtcgaag gggaatttga agcgataaat gatgcggaac tgacagataa
     1681 agaagagtcc gagtccgctt ccgatccgga atcgttaagc gaagttgaag aagcaaaaag
     1741 cgaacctgca gatgtccctg aacaaaagaa agatagaatc gtgccgttta atgtacttat
     1801 gctgaagaat gataaactga aatacgagca acgtgaaaaa agagccggca gccagtctgc
     1861 cgttgacgcc acaaaaaatg atttcacacg cacggaacaa gtaaaggata tcccaatacc
     1921 tgaagagttg gatgaagaac cggaagaaca accttattat gccttccctt caaaagactt
     1981 tcttattcag cctgaagagc atatagaaga tacggaatgg cttgagtccc agtcgaaaaa
     2041 gctggaagag gcgctttcat attttgctgt gcaggcaaat gtcattgaag ccgtgcaagg
     2101 gccaaccgta acgagattcg aattgacggt tggacatgga acgaaggtga gcaaggtccg
     2161 taatctcact gatgatctga aattagcttt ggcagcggaa gacatccgga tccaagcacc
     2221 gatccctggg accagctcca tcgggattga aattccgaac cggaagccaa gagccgtacg
     2281 gatttctgaa gtcattgaaa cagagcggtt ccaaggctcc ggatctccgt tggaagcagt
     2341 cctgggactg agcttgacgg gtgagccggt gacattggat ctacggaaaa tgccccatgg
     2401 aatgattgct ggagcaactg gttccgggaa gtctgtgtgc attaattcaa tcctgatcag
     2461 tttattgtat aaagcgtcgc catcggattt gaaaatgctg ctcatcgacc cgaaaatggt
     2521 ggaacttgca ccatttaacg gaattccgca tttgttgagc cctgtaatca ccgacgtgaa
     2581 ggcagcgact gccgcgttga aatgggcggt cggcgaaatg gaacgccgtt atgagctatt
     2641 tgcgcatgtc ggtgcgagaa atattgagcg ttataatgaa atggcacaga aagaccgcaa
     2701 gttttcattg aagctgcctt atctgcttat cgtcattgat gagctggcgg acttgatgat
     2761 gatggctcca gcggatgtgg aagtttcgat cggccgtatc acccaaaagg ccagggcgtg
     2821 cggaatccat ttgatcattg caacacaacg cccatccgtc gatgtcatta ctggaacgat
     2881 caaggcgaat gtcccgaccc gaattgcatt ttccgtgtcc tcccaaatcg actcccgaac
     2941 aatcattgat acgccgggag ctgaaaggct gctaggaaga ggcgatatgc tttatctagg
     3001 aaacgggcag tcctctgcag tgcggttgca aggtacgttt gtcacggatg ccgaaattga
     3061 acgtataata gaacacgttc agaacgaggc tgagcctgac tatttattcg gccagga
//
LOCUS       DASULI010000306         6412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1046735,
            whole genome shotgun sequence.
ACCESSION   DASULI010000306 DASULI010000000
VERSION     DASULI010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046735"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFX34_06525"
     CDS             <1..859
                     /locus_tag="VFX34_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX5564596.1"
                     /translation="ITTQIEHPSILNACRKLEEHGFEVDYLTVDAQGLISLEELEKKV
                     RKDTILVSIMHINNEIGTIQPIRKCAELIKKNSRAIFHSDCVQSFGKLPVSMDDIGLD
                     AITISAHKVNGLKNSGALLLKKGVTPHAINYGGGQENGLRSGTVSVPNAVAMAKAMRI
                     SAVKEERADYREWRNLLIETCRPYENIRILSPEAGAPHILSIAFKSINGEVAVNYFQE
                     QGIIISTSSACSSKNKNAGHVIEAIGLENKFKNGVIRVSFGNTNTRDEIDEFINVLKG
                     FMVLIERGM"
     gene            863..2068
                     /gene="thiI"
                     /locus_tag="VFX34_06530"
     CDS             863..2068
                     /gene="thiI"
                     /locus_tag="VFX34_06530"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEX5564597.1"
                     /translation="MKWKEILIRYGELSLKGRNRNHFVRKLRNNIKNAMSDLKSVQLR
                     AERDRMFLIADDVKDMEKAIERLPHIFGIQSFSPVAMCEADLAAIKQKALEVIGMEDT
                     AGKTFKVEIKRADKTFPYVTGELQQAIGGHVLHHFPNLTVQMKKPDIMLLVDIRKEGA
                     FLTSKVYKGAGGMPVGSNGHSLLMLSGGIDSPVAGYLLMKRGVSIDAIHFASPPYTSE
                     LAKRKVMDLTEKLCSFGAPVRLHVIPFTELQQAIVQQVPDNLTMTSTRRVMLQIADQV
                     REEIGALALVTGESLGQVASQTLESLTAINEVTATPILRPLIAMDKLDIIELSQQIGT
                     YDISIRPYEDCCTIFTPSNPKTKPKLERVLHFESFFDFQPLIEEAVRTREVIEVWEQQ
                     EKDEFEDLL"
     gene            2153..2362
                     /locus_tag="VFX34_06535"
     CDS             2153..2362
                     /locus_tag="VFX34_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HEX5564598.1"
                     /translation="MANNNSGNSNQLLVPGVQQALDQMKNEIAAEFGVNLGPDSTSRA
                     NGSVGGEITKRLVRQAQQQMKGFQQ"
     gene            2548..4122
                     /locus_tag="VFX34_06540"
     CDS             2548..4122
                     /locus_tag="VFX34_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5564599.1"
                     /translation="MNREELLAPENYNLVSEFEKYATDNAKRALIYVNSNGQEKEITY
                     VELMAKANKAANVFTENGLGKGDVILVMVPRTIDAYVAYIGALKAGIVVIPSSEMLRS
                     SDIEYRLEHSQAKAIVAFESFMDQFESVKNLHGVQLFVIGEAGSGQKSLTRLMESASS
                     DFDGPATKGTDMAFLSYTSGTTGKPKGVVHTHAWAYAHLRTAGPNWLGIEEGDVVWAT
                     AAPGWQKWIWSPFVSVLGSGATGLVYDGKFDVKVYLDLIKKYEVNVLCCTPTEYRFMA
                     KAENLQDYDLSSLRSAVSAGEPLNREVIDIFDRTFNLAVRDGYGQTENTLLVGTMLGM
                     EARPGSMGKPTPGNRVDIIDDEGNPVAAGEVGDIAVHISTPALFKEYLNDPERTKMQF
                     RGEYYVTGDRAKLDEDGYFWFEGRGDDIIISSGYTIGPFEVEDALTKHSAVRECAVVG
                     SPDEVRGNIVKAFIVLRNPDMEHSENLVKELQEHVKTLTAPYKYPRKIEFVKELPKTA
                     SGKIMRVELRKREQNK"
     gene            4233..5150
                     /gene="rarD"
                     /locus_tag="VFX34_06545"
     CDS             4233..5150
                     /gene="rarD"
                     /locus_tag="VFX34_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX5564600.1"
                     /translation="MQSEKSGILWVVGAFLFWGIMPIYWKHLEHVQSDEILTGRIVWA
                     FVSTVLVVLIMKNGRQLIDDIRELWKSQSQFWSLFIATVLISGNWFTYIWAVNHDYIV
                     QTSLGYYFNPLVSVLLGIIFLKEKLSSSQKIAFMLAFTGVIILTVSYGRIPWIALCLA
                     ITFAIYGFIKKNIKLDALRGLAIETLFITPFAIGYYIFLFMKEQAVFLHGDVKTDILI
                     ILTGIATALPLVMYAKGVQMIPLFVAGFIQYIAPTLMLFIGVVVYKESFGGIELLSFS
                     LIWAALILFTVSKVYESISTKRGAHGTLK"
     gene            5214..6218
                     /gene="sppA"
                     /locus_tag="VFX34_06550"
     CDS             5214..6218
                     /gene="sppA"
                     /locus_tag="VFX34_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX5564601.1"
                     /translation="MAMKRWIAVIVAAVLIFISIGINSLSYLFTRDFSGFMEELAAGN
                     VGYSEVVIEQGRSRDRIAVLTLDGVIQDVGSSLFATGGYNHQMFMSQLNDILNDETIK
                     GVVLSVNTPGGGVVESSDIYDAIKAIQIDREIPIYVAMGGLAASGGYYVSAPAEKIFV
                     HPETITGSIGVIMESVNYAKLAEKYGIDFNTIKTGPYKDIMSGSREMTAEEHNMLQEM
                     INDSYERFVEIVAEGRSMSTEQVKKVADGRIMNGRQAIESGLADDFGKMGDVIDAMKS
                     DYELESATVFEYAPMDSFGSLFGVKVGNLFGRNVESELIGKLLSDYDAPRMMYLYGEK
                     "
     gene            6233..>6412
                     /locus_tag="VFX34_06555"
     CDS             6233..>6412
                     /locus_tag="VFX34_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX5564602.1"
                     /translation="MTDHIEDNQPTIDIPEPNRLPEYEIIRTERFRPKFAGFWTRFWA
                     YVIDLLIVSSISGIFI"
BASE COUNT         1873 a         1284 c         1612 g         1643 t
ORIGIN      
        1 cattacgacg caaatcgaac acccttctat attgaatgcg tgtcgtaaat tggaggaaca
       61 cggttttgaa gtcgattatt tgactgtgga tgcccaagga ctcatttcat tggaggaatt
      121 ggagaagaag gttagaaaag acacgatcct cgtcagcatc atgcatatca ataatgaaat
      181 cgggacaatc caaccgattc ggaaatgcgc tgaattgatc aaaaaaaata gccgggccat
      241 tttccattcg gattgtgtgc aaagcttcgg caaattgcct gtcagtatgg acgacatagg
      301 tttggacgca ataacaattt cagcgcataa agtgaacgga ttgaaaaact ccggtgcact
      361 gcttttgaaa aaaggtgtga cgccgcatgc gataaattat ggcggtggcc aagaaaacgg
      421 tcttagaagt ggaactgttt ccgtgccaaa tgctgtcgct atggcgaagg caatgagaat
      481 cagtgctgta aaggaagaaa gggcggatta tcgggaatgg cggaatttgc ttatagaaac
      541 atgccgcccg tatgaaaata tccgaatcct ttcacctgaa gctggtgctc cgcatatttt
      601 gtccatcgcg ttcaaatcga tcaatggcga agtggcagtg aattacttcc aagagcaagg
      661 aatcatcatt tcaacatcaa gtgcatgttc atcaaaaaat aaaaacgccg gacatgtaat
      721 cgaagcaatc ggtttggaaa ataagtttaa aaacggtgtc attcgtgtaa gtttcggcaa
      781 cactaatacg agagacgaaa tagatgaatt cataaatgtg cttaaaggct ttatggtatt
      841 gattgagagg ggaatgtaag agatgaaatg gaaagaaatt ttaatacgct atggtgaact
      901 ttcattaaaa ggacggaatc ggaaccactt cgtacgtaaa ttgagaaata acataaagaa
      961 tgcaatgagt gacttgaaat ccgttcaact ccgtgccgag cgagaccgca tgtttttgat
     1021 tgcggatgac gtgaaagata tggaaaaagc gattgaaagg ctgccgcata ttttcggcat
     1081 ccaatcattt agcccagtcg ccatgtgtga agccgatctc gctgcgatta aacaaaaagc
     1141 gcttgaagtg atcgggatgg aagatacagc aggaaaaaca tttaaagttg aaattaagag
     1201 agccgataaa acattcccgt acgtaacagg tgaactgcaa caagcgattg gtggacatgt
     1261 attgcatcat ttccccaatc taaccgttca aatgaaaaag ccggatatta tgctgctcgt
     1321 cgatatccgg aaggaaggtg cctttctgac gtccaaagtg tataaaggtg caggcggcat
     1381 gccagtcggt tcgaatggcc actcgctgct tatgctatct ggaggcatcg acagtcctgt
     1441 tgcaggatat cttctaatga aacgtggcgt ttcgatcgat gcaatccatt ttgcgagtcc
     1501 accgtatacg agtgagctgg caaaacggaa agtgatggat ttgactgaaa aactgtgctc
     1561 attcggagca ccggtccgtc ttcatgtcat tccgtttaca gaactgcaac aagcgattgt
     1621 ccaacaagtt cccgataact tgacgatgac atctacaaga cgagtgatgc ttcagattgc
     1681 cgatcaagtg agagaagaga ttggcgcatt ggcgcttgtg acaggagaga gtcttgggca
     1741 agttgcaagc cagacacttg agagcctgac tgccatcaat gaagtgacgg ctacacctat
     1801 tctccgccct ttgatcgcaa tggataagct ggacatcatc gaactttccc agcaaatcgg
     1861 aacatatgat atttcaatta gaccttatga ggattgctgt acaatcttta ccccgagcaa
     1921 cccgaaaaca aaaccgaaac ttgaaagggt ccttcatttc gaaagctttt ttgattttca
     1981 gcctttgatc gaagaagcgg tgagaactag agaagtgatc gaagtttggg agcagcaaga
     2041 gaaggacgaa tttgaggatt tactataaag aatagggtat tattaccatt acacctcctg
     2101 catgaatttc cggaaacggt ccattctata tgcacaagga ggtgacaacg acatggctaa
     2161 caacaacagc ggcaactcaa accaactcct agttccaggg gtacaacaag ctcttgacca
     2221 aatgaaaaac gaaatcgcgg ctgaatttgg tgtaaacctt ggaccggact ccacatcgcg
     2281 tgccaacgga tccgtcggcg gagaaattac taagcgatta gtgcgtcaag cccaacaaca
     2341 aatgaagggt ttccaacaat aatgagtatt taaaagctgt cccggatttc cggggcagtt
     2401 tttcttcatg tacgacctgt ttcattacac ctcagtatta acttgcgatg aagagtttat
     2461 tcaaactgtc tgcaagaaaa atgaatatgt tttgaacttt ctacatggcg tttgtataat
     2521 tgaaacatat gaaaaggggg aattcagatg aaccgtgaag aattacttgc acctgagaac
     2581 tataatctcg tttctgaatt tgaaaagtat gctacagaca acgcaaaaag agcactgatc
     2641 tatgtcaatt cgaatggaca ggaaaaagaa attacatatg ttgaattaat ggcaaaagcg
     2701 aataaagccg cgaacgtatt tacggagaac ggcttgggta aaggggatgt catccttgtc
     2761 atggttccga ggacgattga tgcgtacgtt gcatacatcg gagcattgaa ggcgggcatc
     2821 gttgtcatac caagctctga aatgctgcgt tcatctgata tcgaataccg tttggagcat
     2881 agtcaagcga aagcgattgt cgcctttgaa tcattcatgg atcaattcga atccgtgaaa
     2941 aatttacatg gtgtccaatt gtttgtcatc ggtgaagcgg gctctggcca aaaatcgttg
     3001 acccgattga tggagtcggc ctcctcggat tttgacggtc ctgcaacaaa ggggacggat
     3061 atggcattcc tttcgtatac atcaggcacc acaggcaagc cgaaaggggt tgtccataca
     3121 catgcgtggg cttatgcgca tttacgcaca gctggaccga actggcttgg tattgaggaa
     3181 ggggatgtcg tttgggcgac cgccgcacct ggatggcaga agtggatctg gtccccattc
     3241 gtttccgtac tgggcagcgg ggcgacaggc ctcgtctatg atggcaagtt cgatgtgaag
     3301 gtttacttgg atttaattaa gaaatatgaa gtcaacgtct tatgctgcac gcctacggaa
     3361 tacaggttca tggcgaaagc cgagaattta caggactatg acctttcttc ccttcgaagc
     3421 gcggtttccg cgggggagcc gttgaaccgg gaagtaatcg atatttttga taggacattc
     3481 aatctggcgg tcagggatgg ttacggtcag acggaaaata cgcttcttgt cggcacaatg
     3541 ttagggatgg aagctagacc gggatcgatg ggcaagccga caccgggcaa ccgggttgac
     3601 attatcgacg atgaaggcaa cccggtagct gccggagagg taggggacat agccgtccat
     3661 atttccacgc ctgctctctt caaagagtat ttgaatgatc cggaacggac aaaaatgcaa
     3721 ttccggggtg aatattatgt aaccggggac cgtgcaaagc tggatgagga tggatacttc
     3781 tggtttgaag gacggggcga tgacatcatc atcagctcgg gctatacaat cggaccattc
     3841 gaagttgaag atgcattgac gaagcattcg gcggtgcgtg agtgtgcagt cgtcggaagt
     3901 cccgatgaag tccgcggaaa tatcgtcaaa gcattcatcg tattgcgaaa tcctgatatg
     3961 gagcacagtg aaaatctcgt aaaagaattg caggaacatg tgaaaacgtt gactgcccct
     4021 tataaatatc caagaaagat tgaattcgtg aaagagttgc cgaagacggc ctccggtaaa
     4081 atcatgagag tcgaattgag gaagcgagag cagaataaat gaatcactac aaccgtccaa
     4141 ccgccggcat ggcatggacg gtttttcctt ggcgggttta cgaacagttt tgaaaggact
     4201 ataattgtcc tattcgaaag gagggtgcct gaatgcagtc tgaaaaaagc ggtatcctat
     4261 gggtcgtcgg agcttttctc ttttggggaa tcatgcctat ttattggaag caccttgaac
     4321 atgtacaaag tgatgaaatt ttaacgggac ggatcgtatg ggcattcgta tccacagtcc
     4381 tggttgtttt aattatgaaa aatggcagac aactgatcga tgacatccgg gagttatgga
     4441 aaagccagtc gcaattttgg agtcttttca tcgcaaccgt gttgatatcg ggaaactggt
     4501 tcacctacat atgggcggtc aatcatgatt atatcgtgca aacgagtctc ggctattatt
     4561 ttaatcccct tgtctccgtt ttactcggca tcatctttct gaaggagaag ctttcgtcat
     4621 cgcagaagat cgctttcatg cttgccttta cgggtgtcat tatacttacc gtttcatacg
     4681 gaagaattcc gtggatcgct ttatgccttg caattacgtt cgccatttac ggattcatca
     4741 agaaaaacat caaattggat gccctgcgtg gattggctat cgagacatta ttcattacgc
     4801 cattcgcaat cggttattac atcttcctat tcatgaaaga acaggccgtc tttttacacg
     4861 gagatgttaa aactgatatt ctaatcatac ttacaggcat cgcaaccgca cttccacttg
     4921 tcatgtacgc aaaaggagtg cagatgattc cattatttgt agcggggttc atacaatata
     4981 ttgcaccgac gctgatgctg ttcattggcg tagttgttta caaggaatct ttcggcggaa
     5041 tcgaattgtt atcattttca ttgatatggg cagcacttat ccttttcacg gtttccaagg
     5101 tttatgaatc gatttcaact aaaaggggtg ctcacggaac attgaagtaa aatgaaacat
     5161 ttgttagatc ggatacgtat acaatggtgg aaggaagaag ggggaaatac ataatggcaa
     5221 tgaaacgatg gattgcagtc atcgtggcag cagtgctgat cttcatttca ataggcatca
     5281 actcactttc gtatttgttt acgagggatt tcagtggctt catggaggaa ttggctgctg
     5341 gcaatgttgg ctatagcgaa gtggtcattg aacaaggaag aagcagagat cgaattgccg
     5401 ttctcacatt ggatggcgtc atccaggatg ttggcagttc cttatttgct acaggcggtt
     5461 ataatcacca aatgttcatg tcgcagctca atgatatatt gaacgatgaa acgatcaagg
     5521 gcgtcgttct ttcagtgaac acgccaggcg gtggagtggt ggagtcatcg gatatttatg
     5581 atgccatcaa agcgatacaa atcgataggg aaatcccgat ctacgtagca atgggaggat
     5641 tggccgcatc cggcggctat tatgtatccg cgcctgcaga gaaaatcttc gtacatcctg
     5701 agacaatcac aggttcaatc ggggtcatca tggaaagcgt caattacgcc aagctagcgg
     5761 aaaaatacgg aattgacttc aacacgatca aaacgggccc ttacaaagat atcatgagcg
     5821 ggtcccgcga aatgacggcg gaagagcata atatgcttca ggaaatgatc aatgattcat
     5881 acgagcggtt tgtcgaaatt gttgcggaag gccgtagtat gtcgacagag caagtcaaga
     5941 aagtggcgga tggccgcatc atgaatggca gacaggcgat cgaatcgggt cttgctgacg
     6001 attttggaaa gatgggcgat gtaatcgacg caatgaaatc cgactatgag ctggaaagcg
     6061 ctacagtctt cgaatatgcg ccaatggaca gcttcggatc ccttttcggc gtgaaggtcg
     6121 gcaatctttt cggacgcaat gtggagtccg aactcatcgg gaaattgctt tccgattatg
     6181 atgcaccgcg gatgatgtac ctttatggtg aaaaataagg agggaataag gcatgacgga
     6241 tcatattgaa gacaatcaac cgacaattga cattcccgag ccgaatcgcc tccctgaata
     6301 tgaaatcatc cggacggagc ggttcaggcc gaaatttgcc gggttttgga cgcgtttttg
     6361 ggcctatgtt atcgatctgc tgatcgtttc atcgataagc gggatattca tc
//
LOCUS       DASULI010000307         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1214827,
            whole genome shotgun sequence.
ACCESSION   DASULI010000307 DASULI010000000
VERSION     DASULI010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214827"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFX34_06560"
     CDS             <1..306
                     /locus_tag="VFX34_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HEX5564603.1"
                     /translation="KMSIIHHGKQIVSGSLRDVKRSFGRQNVRVRSDHDLTGLDSVPG
                     VLSVAHSVEGAVFQIEEEDVANELLSVALQAGPIRHFEIEEPSLQDIFIAKVGKDYA"
     gene            299..1561
                     /locus_tag="VFX34_06565"
     CDS             299..1561
                     /locus_tag="VFX34_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5564604.1"
                     /translation="MREFWIIFKQAFTTKAKAKSFTITTIIMVAAIFLLANISKIIDT
                     VEDMTGAGDSQNALQVVDTSGMLVDRLQAQLADGDSSLTIEASDESEKTLADQVSSGD
                     IESFLSLSVDGQGTIHANYTTMSMMDFLLPMRIRDALQSIQTEMKAEELSLSGAQVQT
                     VFTPIQFEQKNVSPSAKSEEELNQARVLVYALMFVIYIAVFIYSNMIAMEVANEKSSR
                     VMEILISSVSPVKHMFAKVLGVGTLGILQILIYGVAGYIALKTSATEVPGGFSDFFSL
                     KDVSVSTLIYAAVFFLLGYFLYATLAALLGSLVSRTEDVQQMIMPMSMLIVAAFIISA
                     MGLGNPELGYVTYASYFPFFTPLVMFLRVGMLDLPLWEPLLAIFITLLTIFILGWFGA
                     RVYRGGVLMYGPSRSLKDIRKAIQLGKE"
     gene            1677..>2695
                     /locus_tag="VFX34_06570"
     CDS             1677..>2695
                     /locus_tag="VFX34_06570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX5564605.1"
                     /translation="MSTIRFLHTADLHLDSPFKGMTGLPPARLNELRESTFTAFNRFI
                     DYAVETSPDFILIVGDLYDGEDRSLRAQRKFQEGMERLHAAGIPVYLTYGNHDHLSGN
                     WTRFELPPNVHEFSEEVGEVSLTVKGMDVVVHGFSYPTRHVREEMISRFPVAPDSHSF
                     HIGMLHGSLAGNEAHAVYSPFTIGELQSKHYDYWALGHIHQRQQLSEDPPIVYPGNIQ
                     GRHRNESGEKGFYEVELSKTHATLSFVPTSVVHFGRIQIPCVNIRHANEWLAVCEEAL
                     LSFASEHGPSVVDVEIMNLDDETAAFFAQTAENVWLDTIREVMDGNEPFVWVQRLSVT
                     NPSYR"
BASE COUNT          670 a          604 c          708 g          713 t
ORIGIN      
        1 aagatgagta tcatccacca tggcaagcag atcgtcagcg gctcgctaag ggatgtgaaa
       61 cggtcgttcg gcaggcaaaa tgtacgcgtc cgttccgatc atgatctaac gggactcgac
      121 agtgtaccgg gtgtcctttc cgttgcccat tcggtcgagg gagccgtgtt ccagatcgag
      181 gaggaagatg tcgcgaacga attattatca gttgcattgc aagcgggccc gatccgtcat
      241 tttgaaattg aagagccttc cttgcaagac atttttatcg caaaggtggg gaaagattat
      301 gcgtgagttc tggatcatct ttaaacaggc atttacgaca aaagcaaaag ccaagtcatt
      361 cacgattaca acgatcatca tggttgccgc catattcctc ttggcgaata tatcgaaaat
      421 cattgatacg gttgaggata tgacaggagc aggagattca caaaatgccc tccaagtcgt
      481 cgatacaagc gggatgctcg ttgaccggct gcaagcgcaa ttggcggacg gggacagcag
      541 tctcacgatt gaagcgtccg atgaatcgga aaagacattg gccgatcaag tatcatctgg
      601 agacatcgaa tcattcctca gcctctcagt cgatggacag ggaacgatcc atgcgaatta
      661 tacgacgatg agcatgatgg actttttatt gccgatgcgc atccgggatg cattgcagtc
      721 aatccagacg gaaatgaaag cggaggagct gtccctttct ggtgcgcaag tgcagacagt
      781 gtttacgcca atccagtttg aacagaaaaa cgtctcccct tccgcaaaat cggaagagga
      841 actgaatcag gcgcgagtcc tcgtctatgc actcatgttt gtcatctata ttgcagtgtt
      901 catttattcg aatatgatcg cgatggaagt cgcgaatgaa aagtcttcga gggtcatgga
      961 aatccttatt tccagcgtgt cgcctgtgaa gcatatgttt gcgaaggtgc taggtgttgg
     1021 aacacttggg attctccaga tactcattta tggagttgca gggtatatcg ccttgaaaac
     1081 atctgcaact gaagtgcctg gcgggttttc tgatttcttc agtttgaaag atgtaagtgt
     1141 cagtacattg atttatgccg ctgtgttttt tctgctcggc tatttcctct acgcaactct
     1201 tgctgcattg ctaggctcat tggtgagcag gacggaagat gtccagcaaa tgatcatgcc
     1261 gatgtccatg ctgatcgtgg cggctttcat catctccgcg atgggcttgg gcaatcctga
     1321 actgggctat gtgacatatg catcgtattt cccgttcttc acgccgctcg tcatgttttt
     1381 acgggttggc atgctcgatt tgccgttgtg ggagccattg ctcgctatat tcattacgct
     1441 gctgacgatt ttcatcctcg gctggttcgg tgcccgtgtg taccgcggag gcgtcctcat
     1501 gtatgggcct tcgcgttcat tgaaagatat tcgtaaagcg atccaactag gaaaagaatg
     1561 aagggaagcc gtttttcgtc gggaaattcg acggataacg gctttttcaa tttctattgc
     1621 tctactgtat atggtaaaat tagaacaaat attcgttaag agaggagggg gtctccttgt
     1681 cgacaatcag atttttacat acggcagatt tacatctcga tagcccgttc aaaggaatga
     1741 caggactacc gccagcacgg ctgaatgaat tgagggaaag cacattcacc gcattcaacc
     1801 gcttcattga ctatgcagtg gaaacgtccc cggacttcat tttgattgtc ggggatctat
     1861 acgacggtga agaccgaagc ttgcgtgcac agcggaagtt ccaggaaggg atggagaggc
     1921 tgcatgcagc ggggattccc gtatatctca cttatggaaa ccatgatcat ctcagtggaa
     1981 actggacccg tttcgaacta ccgcccaatg tccatgaatt ttcggaggaa gtgggagaag
     2041 tgtcattgac ggtgaaaggg atggacgtag tggtccacgg cttcagctat ccgacccggc
     2101 acgtccggga agaaatgatc agcagatttc cggttgctcc ggattctcat tccttccata
     2161 tcggaatgtt gcacggcagt ctagccggaa atgaagcgca tgcggtttac tcgcccttta
     2221 cgatcgggga gctgcaatcg aagcattatg attattgggc gctcggtcat atccatcagc
     2281 gtcagcaatt atcggaagac ccgccaattg tctatccagg gaatatccaa ggacgtcatc
     2341 ggaatgaatc gggtgaaaaa gggttttacg aagtggagct cagcaaaacg catgcgaccc
     2401 tttcattcgt tccgacgtcc gtagttcatt tcgggcggat tcaaatcccc tgtgtcaata
     2461 tccggcatgc gaatgagtgg cttgctgttt gcgaagaggc tttgctttcg ttcgcttcag
     2521 aacatggtcc atctgtcgtt gatgttgaaa tcatgaatct cgacgatgaa acggccgcct
     2581 tttttgcgca gacagccgaa aacgtttggc tggacacaat ccgtgaagtg atggatggga
     2641 atgaaccttt tgtgtgggta cagcgtttga gcgttacaaa tccttcctat agaca
//
LOCUS       DASULI010000308         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_572908, whole
            genome shotgun sequence.
ACCESSION   DASULI010000308 DASULI010000000
VERSION     DASULI010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572908"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VFX34_06575"
     CDS             complement(<1..868)
                     /locus_tag="VFX34_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225990.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEX5564606.1"
                     /translation="MADLNYPFPTEGKRYYTWSRHLKDEFGMKIFKVALDAGFDCPNR
                     DGTVAFGGCTFCSVAGSGDFAGDRAKPIEVQFEEIRDRMHQKWREGKYIAYFQAYTNT
                     HAPLPVLKKKFEAALAQPGVVGLSIGTRPDCLPDDVVDYLAELNERTYLWVELGLQTV
                     HEKTAKLVNRAHDYATYVEGVEKLRRHGIRICTHIINGLPLEDTEMMIETAKEVAKLD
                     VQGIKIHLLHLLKGTPMVKQYEKGMLQFLGRDEYISLVVDQLEILPPNMIIHRITGDG
                     PLDLMIGPMWSAS"
     gene            1042..2214
                     /locus_tag="VFX34_06580"
     CDS             1042..2214
                     /locus_tag="VFX34_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5564607.1"
                     /translation="MPNKVWLLIIGMFVNVIGNSFLWPLNTIYMHEYLGKSLAVAGLV
                     LMANAGAGVVGNLLGGFLFDRIGGFKSIMAGIIISILALAYLVYDHDWYPYIILLTVL
                     GFSGGIIFPSMYAMVGTIWPEGGRKAFNSIYLAQNVGVAIGPALAGLVASVHIDYIFS
                     ANLIFYVIFLFIAYFGYKKLEIAPDRHTDVIRETARIKNKAPFYALLILVGGYLITWL
                     VYSQWSTTISTHALSLGVTLRQYSFIWTINGLLIVIGQPLIKPLIKRLEKQLKAQMVI
                     GTVIFIISFIVVSFAGSFKMFIVSMVILTFGEMIVWPAIPTLASQLAPKGRDGFYQGV
                     VNSAATIGRMIGPLAGGILVEIHGMKMMLLLLTSLMAITIVTTLLYDRPLQKREIQ"
     gene            2324..>2662
                     /locus_tag="VFX34_06585"
     CDS             2324..>2662
                     /locus_tag="VFX34_06585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5564608.1"
                     /translation="MKPIYSTAMEAISQIEDGSTLMVGGFGLVGIPEQLILALVEKGV
                     KDLTIISNNCGVDEWGLGLLLKEKQIKKMIGSYVGENKEFERQVLSGEIEVELTPQGT
                     LAEKIRAGGAG"
BASE COUNT          656 a          622 c          642 g          742 t
ORIGIN      
        1 tcgatgcgct ccacatcggg ccgatcatca aatcgagcgg accgtccccg gtaatccgat
       61 gtatgatcat gtttggcgga agaatttcaa gctgatcgac gacaaggctt atatattcat
      121 cccggccaag gaattgcagc atgccctttt catattgctt caccatcgga gttcctttta
      181 atagatgcag caaatggatc ttaataccct gcacatcaag cttcgccact tcttttgccg
      241 tttcaatcat catctccgtg tcttcaagtg gaagaccatt gatgatatgg gtgcatatgc
      301 ggatgccgtg cctccgcaat ttctcaacgc cttccacgta cgtggcgtaa tcatgcgctc
      361 tgttcaccaa ctttgccgtt ttttcatgga cggtctgcaa gcctaattcg acccataaat
      421 acgtccgttc attcagttcg gccaaatagt cgacgacatc atccggaagg caatcgggac
      481 gcgtgccgat cgataggcca acgacacctg gttgggcaag tgccgcttcg aatttctttt
      541 tcaagacggg aaggggggca tgtgtattcg tgtacgcttg aaaataagcg atgtacttgc
      601 cttctctcca tttctgatgc atccgatcgc gtatttcctc gaactggact tcgatcggct
      661 ttgcccgatc tccggcaaaa tcacctgaac cggcgacact gcaaaatgtg cagccgccga
      721 aagcgactgt accgtcccga ttcggacaat caaatcccgc atcaagggca accttgaata
      781 ttttcatccc gaactcgtcc ttcaaatgtc tggaccatgt ataataccgt ttcccttcag
      841 tagggaacgg gtaatttaag tctgccatct gtttcaaccc ctctaatcgt acattgtatc
      901 atgacagatt caatcagtgt catctgaatt gcggaacata ttctgtttca gttgttcgaa
      961 accagaaaaa ggcatacact cgaaatagca ttagcttgca agagatagag attggaggca
     1021 atgaaaatgc ctaaaatgga aatacccaat aaagtttggc tgctcatcat aggaatgttt
     1081 gtcaatgtca tagggaactc cttcctttgg cctttgaata cgatatatat gcatgaatat
     1141 ctcggtaagt ctttggcagt cgccggtctt gtcctgatgg cgaatgccgg tgccggtgtc
     1201 gtcgggaacc tgcttggcgg ttttttattt gaccggatcg ggggtttcaa gtcgattatg
     1261 gcgggcatca tcatttcgat tctggcgctt gcctatcttg tatacgatca tgattggtat
     1321 ccttatatca tcttgctgac tgttttaggt ttcagcggcg gtatcatttt tccgagtatg
     1381 tatgcaatgg tcggaacgat atggcccgaa ggcggcagga aggcattcaa ctcgatctat
     1441 ttggcgcaga acgtcggtgt cgccatcggg ccggcgcttg caggtcttgt cgcttccgta
     1501 catatcgact atattttttc ggccaacttg atcttctatg tcatttttct tttcattgcc
     1561 tatttcgggt ataaaaagct ggaaattgcg ccggatcgtc atacggacgt catccgggag
     1621 acggcgcgga tcaagaacaa ggcgcctttc tatgctttgc tcattttggt cggcggctat
     1681 ttgatcacat ggctcgtcta ctctcaatgg tcgacgacga tttccacgca tgctttatca
     1741 cttggcgtga cattaaggca atacagtttt atttggacaa tcaacggctt gctgattgtc
     1801 attggacaac cgcttatcaa accgctcatc aaacggcttg agaaacagct gaaagcacag
     1861 atggtcatcg ggactgtcat cttcatcatt tccttcatcg tcgtcagttt tgccggttct
     1921 ttcaaaatgt tcatcgtttc gatggtcatc ctcacattcg gtgaaatgat cgtttggccg
     1981 gccatcccga cattggcgag ccagcttgca ccaaaaggaa gggatgggtt ttaccaaggg
     2041 gtcgtcaact ccgccgcaac aatcggtcgg atgatcggtc cattggccgg gggaatcctc
     2101 gtggaaatcc acggtatgaa aatgatgctt ttgctgctta cgagcctaat ggctataaca
     2161 atcgtcacaa cgcttttata cgatcggcct ctccaaaaac gggaaatcca ataaggattt
     2221 tccgtttttc cagtttggaa cgttgcgtcc cgctgaatag tttgattaaa tgaatagtgg
     2281 ataggtgcaa tttttctgaa ggatttaaaa gggggaatgc tagatgaaac cgatttattc
     2341 aacagctatg gaagcgattt cgcagatcga agacggctcg acgctaatgg tcgggggctt
     2401 cggacttgtc ggaatcccgg aacagctcat tttggcactc gtcgaaaaag gcgtcaaaga
     2461 tttgacgatc atttcgaata actgtggtgt agacgaatgg ggtcttggcc ttctattgaa
     2521 agaaaagcag attaagaaga tgatcggctc ctatgtcgga gagaacaaag agttcgaacg
     2581 tcaagtacta tcgggcgaaa tcgaagtcga actgacaccg caagggacgt tagcagaaaa
     2641 gatccgggca ggcggtgcgg ga
//
LOCUS       DASULI010000309         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_962196, whole
            genome shotgun sequence.
ACCESSION   DASULI010000309 DASULI010000000
VERSION     DASULI010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962196"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VFX34_06590"
     CDS             <1..284
                     /locus_tag="VFX34_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564609.1"
                     /translation="MWHFWGEKEEFNGKLKVVGINEKGEEHKILIRNAGTLNSEKVWE
                     YQEVGMNPNNGADTHVPSNMEFPTPGLWKLKVYIGDKLFGEIVVNVLES"
     gene            547..1653
                     /locus_tag="VFX34_06595"
     CDS             547..1653
                     /locus_tag="VFX34_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564610.1"
                     /translation="MQVVNWERMVIIYGTITEYMGTAYTGNGVAHKFDTLIATADKTI
                     FLKAPPTNAISKVLNDAAAHFIKRGYDVDRFNNPVEPDNTDAIFVKDLNLFILQASHP
                     VPLEPTEIGGRHHVISFYDAYDEEKLQRRATEMAAIAGESNLVFTKMMQAMSDALLIH
                     DDWEKVNIVRMMWHEHEQMTESLKNELFGTMKLHKKSTVSHRIAGSLSPGGARDYIPS
                     ITSWTKRRILMKGLSGTGKSTMLKTLGKEAERRGIDVLYGWCGLDPTSVDILLLPELS
                     VCLFDATQPHEYDPESPLDEILDLVSLCTEDAEAEEKILEISGRYKEKIMDATGYMHA
                     YALSENRLREIVDQAIIRKSFEEKARQLSKWIDV"
     gene            1726..>2536
                     /locus_tag="VFX34_06600"
     CDS             1726..>2536
                     /locus_tag="VFX34_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5564611.1"
                     /translation="MSKVLDAFLQENLQELRDQGLYNVIDPVEGPNGPVIKINGKDLI
                     NLSSNNYLGLATDEDLKKLAIAATEKYGVGAGAVRTINGTLDIHIELEKKLAEFKGTE
                     AAISYQSGFNCNMAAISAVMNKQDAILSDELNHASIIDGCRLSGAKIIRVKHQDMDDL
                     RAKAKEAKESGQYGKLMYITDGVFSMDGNIAKLPEIVEIAKEFDLITYVDDAHGSGVT
                     GKGKGTVKHFGLEKEIDFQIGTLSKAIGVVGGYVAGTQALIDWLKVRSRPFL"
BASE COUNT          751 a          529 c          642 g          614 t
ORIGIN      
        1 atatgtggca cttttgggga gaaaaagaag agtttaatgg aaagttaaaa gttgttggca
       61 ttaacgaaaa aggagaagaa cacaaaatcc ttattagaaa tgcaggaacg cttaatagtg
      121 aaaaagtttg ggagtatcaa gaagttggaa tgaatcctaa taatggagct gatacacacg
      181 ttccatcaaa tatggaattt ccaacaccag gactatggaa attaaaagtt tatattggag
      241 ataaattatt cggtgaaatc gttgtaaatg tattagagag ttaacccgat ttttcaattc
      301 aaaataggcg ctactaacta tataaccttt tgctgtccta aaggatgcct atacatcaaa
      361 aactatacat caaaaaacgc tcagaaatct gagcgttttt atttgttttt tgtactaccg
      421 attcactgct gaaatatatg ctaattgggt tgttatttgc gttgtttttt gcagttttct
      481 ccccatcaac cgaacccgtt agcagacaag ccgtgctttt ttttattgtc tgggcatatg
      541 gtagaaatgc aagtagtcaa ttgggaaagg atggtcatca tatacgggac aataacggaa
      601 tacatgggga cagcctatac tgggaacggg gtcgcgcaca aattcgacac attgatcgcc
      661 accgctgaca aaacgatttt tctaaaagct ccgccgacaa atgcaatatc caaagtattg
      721 aacgacgcgg cagctcactt catcaaaagg ggctatgacg tagatcgctt caacaatccc
      781 gtcgagcctg acaatacgga tgccatattt gtaaaggacc tgaatttatt cattctccaa
      841 gcatcccatc ctgtgccgct ggagccgacc gaaatcggcg gccgccacca cgtcatcagc
      901 ttctatgatg cgtatgacga ggaaaagctg caaagacgtg caaccgaaat ggccgccata
      961 gcaggggaat caaacctcgt atttacaaaa atgatgcaag cgatgtccga tgcattgctg
     1021 atccatgatg attgggagaa agtgaatatc gtcaggatga tgtggcatga acatgaacag
     1081 atgaccgaat cactgaaaaa tgaactgttc ggaacgatga agctgcataa aaagtcgaca
     1141 gtatcgcatc ggatcgccgg ctctttatcg ccgggcggcg cacgggatta cattccttcc
     1201 atcacaagtt ggacgaaaag gaggatcctt atgaaaggat tgtccggaac ggggaaatcg
     1261 acgatgctga aaacgctcgg caaagaggcg gaacgtcggg ggattgacgt gctctatggt
     1321 tggtgtgggc tcgatccgac tagcgttgat atcttgctgc tgccggagtt gtcggtctgc
     1381 ctgttcgatg cgacccagcc gcatgaatat gatcccgaaa gtccgttgga tgaaattctt
     1441 gatcttgtct cgttgtgtac ggaagatgcg gaagcggaag agaaaatcct tgaaatcagt
     1501 ggcagataca aggagaaaat catggacgcg accggctata tgcatgccta tgcgcttagt
     1561 gagaacaggc tgcgcgaaat cgtcgatcag gcgatcatcc gaaagtcctt cgaggaaaag
     1621 gcacgtcaac tttcaaaatg gatcgatgtg taaggcggtt ccttctgcgt gttttgcccg
     1681 cttatgctaa aatgggggca tcaaccgttt gaaaggaaga tcattttgtc aaaagttcta
     1741 gatgcatttt tacaggaaaa tttacaggaa ctgcgcgacc aaggcctcta caatgtaatc
     1801 gacccggtgg aaggtccgaa tggtccggtt attaaaatca acggaaaaga tttgatcaat
     1861 ctttcctcga acaactatct ggggcttgcg acggacgaag atttgaagaa gcttgcaatc
     1921 gcggcgacgg aaaaatacgg cgtcggcgca ggtgctgtcc ggacaatcaa cggaacacta
     1981 gatatccata ttgagcttga gaagaaactt gcagaattca aagggacgga agcggcaatt
     2041 tcctaccaat ccggcttcaa ctgcaatatg gctgcgattt ccgcggtcat gaataaacag
     2101 gacgctattc tttcggatga attgaaccac gcatcgatca tcgacggctg ccgtctttcc
     2161 ggtgcgaaaa tcatccgcgt caagcaccag gacatggacg atctgcgcgc gaaagcgaaa
     2221 gaggcgaaag agtccggcca atacggcaag ctgatgtata ttacagacgg cgtattttcg
     2281 atggacggaa atatcgcgaa gcttcctgaa atcgttgaga tcgcgaaaga attcgatctg
     2341 atcacgtatg tcgacgatgc gcacggatcc ggcgtaacag gcaaagggaa agggactgtg
     2401 aagcatttcg gtctcgaaaa agagattgat ttccaaatcg gcacattgtc gaaagcgatc
     2461 ggcgttgtcg gcggctatgt tgccgggacg caggcgttga tcgactggct gaaagtgcgc
     2521 tcccgtccgt ttctat
//
LOCUS       DASULI010000310         5218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_397622, whole
            genome shotgun sequence.
ACCESSION   DASULI010000310 DASULI010000000
VERSION     DASULI010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397622"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFX34_06605"
     CDS             <1..1142
                     /locus_tag="VFX34_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5564612.1"
                     /translation="EIDTLGGHTEAAGKIGKLMLDTDMEIIAYINNRAISAGALIALY
                     ADKIYMSPTGVIGASQVVVASGQAADDKAHSLWVAEMINAAKSHERKTIYAEAMAEPS
                     IHLPSYRAGKGELLTLTASEAAEPEVGYSDGTVSSFQEVLKLNNLENAEIVKTNVTFT
                     EGIARFVTHPIVVPILLSIAGLGLVVELYSPGFGVPGTMALSALLLFFFGHLIAGLAG
                     YETLILFIIGLGLIVLEFFIAGGIAGVLGAVAVVVSIIMAGGNPMFMAYSVLIAIAVA
                     ISGMVIIMKFFGRRLHLLNKVVLMDSTDTESGYVSNVNRVELLGKKAITITPLRPSGT
                     IDMEGERIDVVSQGSYIDRGKHVIIVKVEGSRIVVREFEEKGENE"
     gene            1142..2161
                     /gene="floA"
                     /locus_tag="VFX34_06610"
     CDS             1142..2161
                     /gene="floA"
                     /locus_tag="VFX34_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403140.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HEX5564613.1"
                     /translation="MDLIAGAGAGGLIVTGGIVILAIIILSVFFTLVPVTLWISALAA
                     GVRVSIFTLIGMRLRRVIPSRVVNPLIKAHKAGLNVSINQLESHYLAGGNVDRVVNAL
                     IAAHRANIELSFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKAR
                     ITVRANIDRLVGGAGEETVVARVGEGIVSTIGSSVDHAKVLENPDLISQTVLAKGLDS
                     GTAFEILSIDIADVDIGKNIGAELQTEQAMADKNIAQAKAEERRAMAVANEQEMKAKV
                     QEMRAKVVGAEAEVPLAMAEALRTGNIGIMDYMNYKNIQADTGMRDSISKIGVDQQKP
                     DTTKE"
     gene            2199..2690
                     /locus_tag="VFX34_06615"
     CDS             2199..2690
                     /locus_tag="VFX34_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564614.1"
                     /translation="MEILIFVALGIISMLFKGKKPEEAPKTQRRQPNAQTGAPDPMRK
                     LKEMTQEMYKEIQREFQTEIDEPPSRQSSRPESAQTLSLPQKAKEMERRDRTREFPVQ
                     KETKQERSSKRETHRGRLSVHQGSLVLKEPVENHEMIPTNEQDLIKGIIFSEILGPPK
                     SKQ"
     gene            2739..2996
                     /locus_tag="VFX34_06620"
     CDS             2739..2996
                     /locus_tag="VFX34_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564615.1"
                     /translation="MKKLFKMNSSVTIEEFVSVRITGPYSLLKLGPDYAVIRSGEHII
                     EAAGEDLIVETLSEEVAIFSFETLKNVIIKEMKNGQGPYDS"
     gene            2986..4074
                     /locus_tag="VFX34_06625"
     CDS             2986..4074
                     /locus_tag="VFX34_06625"
                     /inference="COORDINATES: protein motif:HMM:NF018591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HEX5564616.1"
                     /translation="MIHKRYIVVLTGKTDPARFLSFLASSGVKVSGIMESGKGVRFRT
                     DKKGIQVIRKHRRRYKLKISIKPADEGTVEKRVFSSFRFLIACIIPLLASLFLWKIEI
                     ETDVPEVSERISGKLEETSITMLRPLHLIPDEGEIRRALMVEDPSLSWVRFKRSGSTL
                     TVIPMLSPKLNDSVEKKGPPSDLIARTGGVITGFQLSRGERVAHVHQTVKKGDLLATG
                     ILEQGNKRTVVGAEGSVYADFWMEYSFKIPKTVDYNLLGEENVIIHWRSPIVWKKGGM
                     PTLQSFMRTERFREDRVDQFELTEGMEEKVIIPLLKYKILTETQSDLIIKDENILHLT
                     FGSDTVEGTILFLMNENIAIKRPLSQGD"
     gene            4080..5039
                     /locus_tag="VFX34_06630"
     CDS             4080..5039
                     /locus_tag="VFX34_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5564617.1"
                     /translation="MSEQLIQLHIEEPNEAVMLLGISDQNMKLIEESMDISILTRGDT
                     ISLAGEEQDIIAGKYLLEQLLKVIRKGININLRDISTAIEMVKNGTIEYFAELYDEEI
                     ARTAKGKAIRAKTIGQREYIHAIRKNDLVFCIGPAGTGKTYLAVVLAVQAMKTGSVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDALHDVLGAEHTERLIERGVIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTKAQMKMFLTRLGFGSKMVITGDKTQIDLPRGVESG
                     LNASEAILKKVPEIHFQYLEQGDVVRHPIVAKIIEAYENEQSS"
BASE COUNT         1499 a         1072 c         1363 g         1284 t
ORIGIN      
        1 ttgaaataga tacgcttggc ggccatacag aagctgcggg gaaaatcgga aagctaatgc
       61 tggatacgga catggaaatc attgcctata ttaataatag ggcaatttcc gctggagccc
      121 tcatcgcttt gtatgcggat aaaatttata tgtccccaac tggcgtaata ggtgcttccc
      181 aagtggtcgt agcgtccggg caagctgccg atgataaagc acacagtctt tgggtagctg
      241 aaatgataaa cgccgcgaag tcgcatgaac gtaaaacgat ttatgcggaa gcaatggctg
      301 aaccttctat acatttgcca agttatcgtg ctggaaaggg cgaattattg accttgactg
      361 caagcgaagc tgcggaaccg gaagtagggt acagtgatgg aactgtttct tctttccaag
      421 aagttttgaa actgaacaat ttggaaaacg ctgaaattgt aaaaacgaac gtgacgttca
      481 cggaaggcat cgcgcggttc gtcacacatc cgatcgtcgt gccgatcctg ttgtcgattg
      541 caggtcttgg cctcgtcgtg gagttgtatt caccaggctt cggcgtacct ggaacgatgg
      601 cgctttccgc attgttgctg ttctttttcg gccatctcat tgcgggtcta gccggatatg
      661 aaacattgat tttattcatt atcggtttag ggctcattgt acttgaattt ttcatagcgg
      721 gtgggatagc tggcgtcctt ggggcggtcg ctgtcgttgt aagtatcatt atggcgggtg
      781 gcaatccgat gttcatggcg tattccgtcc tgattgcgat tgctgtggcc atcagcggga
      841 tggtgatcat tatgaagttt ttcggtagaa ggttgcactt gctgaacaaa gtcgtcctga
      901 tggattcgac ggacacggaa agcggttatg tgtcgaatgt caatcgggtc gagcttctcg
      961 gcaagaaggc gattacaatc acacctcttc gtccttcggg aacaatcgat atggaaggcg
     1021 agcgaatcga tgtcgtttcg caaggaagct atatcgatag aggaaaacat gttatcattg
     1081 taaaggtcga aggatcccgg attgtcgttc gggaattcga agaaaagggg gaaaatgaat
     1141 aatggatcta attgctggag ctggagctgg aggtttgatt gtgacaggtg gaatcgttat
     1201 cctggcgatt atcatattgt ctgtattttt cacgctcgtg cctgtaacac tatggatttc
     1261 cgcactggca gcgggtgtgc gtgtaagtat tttcacattg atcggtatgc gtcttcgtcg
     1321 ggtaatcccg agcagagtcg tcaatccgtt gatcaaggcg cataaagcag gattgaatgt
     1381 ttcgatcaac cagcttgaaa gccattactt ggcaggcgga aatgttgacc gcgtcgtcaa
     1441 tgcattgatt gctgcacatc gtgcgaatat cgaattgtca ttcgaacgtg ccgcggcaat
     1501 tgacttggcg ggccgtgatg ttcttgaagc ggttcaaatg tcagtaaacc cgaaagtcat
     1561 tgaaacacca ttcatcgctg gtgttgcaat gaacggtatc gaagttaaag cgaaagcgcg
     1621 gatcactgtc cgtgcaaaca tcgatcgcct tgtcggtggt gcaggcgaag aaactgtagt
     1681 cgcacgtgtc ggggaaggta tcgtttcgac gatcggttct tccgtagacc atgcaaaagt
     1741 attggaaaat cccgatttga tctcccagac ggttttggcg aaaggccttg actccgggac
     1801 ggcttttgag attttgtcca tcgatatcgc cgacgttgat atcggtaaaa acattggtgc
     1861 tgaacttcaa actgaacaag cgatggctga taagaatatt gcacaggcga aagcggaaga
     1921 acgccgtgcg atggctgtag cgaatgaaca ggaaatgaag gcgaaagtcc aagagatgcg
     1981 tgcgaaagtg gtcggggcag aggcggaagt tcctcttgcg atggcggaag cgcttcggac
     2041 aggaaacatt ggcatcatgg actatatgaa ctacaaaaat atccaggctg acacgggaat
     2101 gcgtgactcc atcagtaaaa tcggcgtgga tcagcaaaaa cccgacacaa cgaaagagtg
     2161 acgtttgagc atataattcc cggaaagggg atgcacagat ggaaatactg atttttgtcg
     2221 ccctcggaat catcagtatg ctcttcaaag gtaaaaagcc ggaagaggca ccgaagacgc
     2281 agaggagaca accgaacgcg cagacgggtg ctcctgaccc gatgcggaaa ttgaaagaaa
     2341 tgacgcagga gatgtacaag gaaatccagc gtgaatttca aacggaaatt gatgaaccgc
     2401 cgagcaggca gtcatcccgg cccgaatctg ctcaaaccct ttcattgccg caaaaggcga
     2461 aagaaatgga gagaagggac agaacaaggg aatttccggt ccaaaaagaa acgaagcaag
     2521 aaaggtcgtc aaagcgggaa acgcatcgtg gccgcttatc agttcaccaa ggcagcctcg
     2581 ttttgaaaga gcctgtggaa aaccatgaga tgattcctac aaatgaacaa gacttgatca
     2641 agggcatcat tttctctgaa attttgggtc cgcccaaatc aaaacaataa tcattgaccc
     2701 cctatccaaa tcatacgctt ggataggggg tttttatctt gaaaaaatta tttaaaatga
     2761 actcatctgt aacgattgaa gaatttgttt cggtcaggat cacaggccct tattcattat
     2821 tgaaactggg acctgattat gcagtgatcc gaagtgggga acatatcatt gaagctgctg
     2881 gggaggatct aatcgttgaa acattatcgg aagaggttgc gatattctca tttgaaacat
     2941 taaaaaatgt gattatcaaa gaaatgaaaa atggacaggg tccctatgat tcataaacga
     3001 tatatcgtcg tcctcacagg taaaacggac ccggcaagat tcctatcgtt ccttgcaagc
     3061 tcgggtgtga aggtttccgg gataatggaa tccggaaaag gagtccgatt ccgcacggat
     3121 aaaaagggca ttcaagtcat aaggaaacat aggcggcgct ataaattaaa aatatcaatt
     3181 aaaccggcgg atgaaggaac agtggagaaa agggtcttct cctctttccg ttttctcatt
     3241 gcatgcatca ttcctctcct tgcatcacta tttctttgga agatcgaaat tgaaaccgat
     3301 gttcctgaag tatcggaacg gatcagcggc aagttggagg aaacgtcgat caccatgctc
     3361 cgcccccttc acttgattcc ggatgaaggg gaaatccgaa gggcgcttat ggtggaggat
     3421 ccatccctct cctgggttcg gttcaaaaga tccggaagca cattgacagt aattccgatg
     3481 ctctctccaa aattgaatga cagtgttgaa aagaaggggc cgccgtccga tctcatcgca
     3541 cgaacaggtg gagtcataac cggttttcaa ctgagcagag gggagagggt cgcccacgta
     3601 caccagactg tgaaaaaagg ggatctactg gcgacgggca tattggagca agggaataaa
     3661 cgaacagtcg tcggagcaga aggctctgtg tatgcagatt tttggatgga atactcattc
     3721 aaaattccta aaacagttga ttacaatctt ctcggggagg aaaatgtcat catccattgg
     3781 agatccccga ttgtatggaa gaaaggcggc atgcccactc tgcagtcgtt catgaggacg
     3841 gaaagattcc gcgaagaccg tgtggatcaa ttcgagttga cggaaggcat ggaggagaaa
     3901 gtcatcattc cgttgctgaa atacaagatt ttaacggaaa cccaatccga tctcatcata
     3961 aaagatgaaa atattttaca cttgacgttt ggaagtgata cagttgaggg gactatatta
     4021 tttttaatga acgagaatat agcgataaaa agaccgttat cccaaggaga ctgaaactat
     4081 tgagcgaaca actgattcaa cttcatatcg aggagccgaa tgaggcggtc atgcttctgg
     4141 gcatttccga tcaaaatatg aaattgattg aagaatccat ggatatttcc atccttaccc
     4201 gcggagatac gatttcactg gccggagagg aacaggatat cattgcaggg aaatatctcc
     4261 ttgagcagct attgaaagtg atccgtaaag gcatcaatat caatttacgg gatatctcca
     4321 cagcgattga aatggtgaaa aacggtacga ttgaatattt cgcagagctt tatgatgagg
     4381 aaattgccag gactgcaaaa gggaaggcaa tacgggcgaa gacgatcggc cagcgggaat
     4441 atatacatgc catccggaag aatgacctcg tcttttgtat cggacccgcc ggtaccggta
     4501 agacgtatct tgcagtagtt cttgccgtgc aggcaatgaa gacggggtcc gtcaaaagga
     4561 tcattctgac aaggcctgcc gtcgaagcag gggaaagcct cggttttctg cccggtgacc
     4621 tgaaagagaa ggtggatcca tatttgcgtc ctctctatga cgccctgcat gacgttctag
     4681 gggcggaaca tacagaacgg ctcatagaac gtggagtaat cgaaatcgct cccctggcgt
     4741 atatgagggg ccgtacgctt gatgatgcgt ttgtcatcct ggatgaggcg cagaatacga
     4801 cgaaagcgca aatgaaaatg tttttgaccc gactcggatt cggctccaaa atggtgatta
     4861 caggcgacaa gacacaaatc gatttgccgc gaggcgttga atccgggttg aacgcctccg
     4921 aggctatatt gaaaaaagtt cctgaaatcc atttccaata cttggagcag ggcgatgttg
     4981 tccggcatcc gattgttgcg aaaatcatcg aagcatacga aaacgagcag tcatcctaaa
     5041 atgactgctc tttcatcatt tcgaaaataa tgaacagata aagagggata ggggggattc
     5101 tttgtgttcg cacccatcat taaacttttc agatccttga aattcaaata tttatcactg
     5161 ttcgtaatcc tattggcagc tatcgcatta ttttccctca tgtacggaaa tgtaaaac
//
LOCUS       DASULI010000311         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1312624,
            whole genome shotgun sequence.
ACCESSION   DASULI010000311 DASULI010000000
VERSION     DASULI010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312624"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1040
                     /locus_tag="VFX34_06635"
     CDS             33..1040
                     /locus_tag="VFX34_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX5564618.1"
                     /translation="MRSRLLAFLVIALTLALAACGAPSKEEVMKKLSGKWTDSKGYEL
                     KATMEIKTGAEPRLYNVDVWHTKPDFYRVSVSQDGEGDAQMIVRNQEGVFVVTPSLGK
                     TYKFQSDWPAQNSQAYLIGALSEDIKADKKAVMTENDKSYVFKTATRNNHKNVLPTQE
                     IHIDKKTLLPTHVSVMNENNEEKIRITFKKITLGTDRKPSDYAVERDSQDMDSGEKPD
                     VADEKTGLRVYYPSLNWEGVTLVDEVNVKTNEGTRAFLTYGGKKDFVIVQEPSLPTPP
                     KMHVSVEGDPVDLGFTVAALTDKSIRWEYGGVSFFLASETLTKDEMIQVASSMVAPEM
                     K"
     gene            1111..2238
                     /gene="alr"
                     /locus_tag="VFX34_06640"
     CDS             1111..2238
                     /gene="alr"
                     /locus_tag="VFX34_06640"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5564619.1"
                     /translation="MEQTTFYRPTKATVQLDAIRQNVKNLQQYLGAETRVIAVVKADG
                     YGHGEVEVAKAALEAGAIMAAVATPDEAVRLRKGGVKGDILVMGPAPASFAKFAAEEE
                     ITVTATGVDWLTDVLAGTPLSKSLKIHVKIDSGMGRIGIREEEELKTFYSLAKEAENI
                     ILDGIFTHFACADEENPTATEQQFNRFMELVDVLPEKPRLVHASNSAASLLYPTYALD
                     AVRFGVGLYGIAPSDYVEQNLPFPLVRALTLKTKLAFVKKLPANSKISYGGTYETGGD
                     EWIGTLPIGYADGLRRGLRGQEVLIGGERVPIVGTICMDQCMVRLPKEMPVGEEVVLI
                     GQQGKEEITMEEWADRLQTIPYEIAVSMAKRVPREYIGKTE"
     gene            2386..2670
                     /locus_tag="VFX34_06645"
     CDS             2386..2670
                     /locus_tag="VFX34_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX5564620.1"
                     /translation="MLRARKNMKEIIVKIPKQMLNENEHTVVHLEQDRGDFVYISTKR
                     YVTNHEPNVIREAMMKGYLEMSQINLRIAKECLHVEYEAQHTVERLVSGG"
     gene            2675..>2912
                     /locus_tag="VFX34_06650"
     CDS             2675..>2912
                     /locus_tag="VFX34_06650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5564621.1"
                     /translation="MAIKRGDVFFADLSPVVGSEQGGTRPVLVIQNDIGNRFSPTVIV
                     AAITAQIQKAKLPTHVEIDAERYGFERDSVILLEQ"
BASE COUNT          896 a          592 c          738 g          686 t
ORIGIN      
        1 gtacccgact gaaatgagaa aggatgaact gtttgcggag ccgtttacta gcttttcttg
       61 tgatcgcatt gacccttgcc ctggcggcat gtggagcacc atcgaaagag gaagtaatga
      121 aaaaactgag tgggaaatgg acagattcaa aagggtatga gctgaaggct accatggaaa
      181 ttaaaacggg agcagagcca aggctataca atgtagatgt ctggcatacg aagcctgatt
      241 tttatcgtgt cagtgtatcg caagatggtg aaggagacgc gcaaatgatt gttcgcaatc
      301 aagaaggtgt cttcgtcgtt acaccatccc ttggcaaaac atataagttc cagagcgatt
      361 ggccagctca aaacagtcaa gcctacctga tcggtgctct atcggaagat attaaggcgg
      421 ataaaaaagc tgttatgaca gaaaatgata aatcctatgt atttaaaact gctacacgaa
      481 ataaccacaa gaatgtcctg ccaacccaag aaatccacat tgataagaaa acacttctac
      541 ctacacatgt atccgttatg aatgaaaaca acgaagaaaa aattcgtatt acctttaaga
      601 agataacact cgggactgat cgcaagccgt ccgattatgc agtggaacgt gactcgcaag
      661 acatggattc gggtgaaaag cctgacgtcg ccgacgaaaa aactggcttg cgcgtctatt
      721 atccttcact caattgggaa ggcgtcacgt tggttgatga agtgaatgtc aaaacgaatg
      781 aaggaacgcg tgcgtttttg acatacggag gaaagaaaga tttcgtcatt gtccaggagc
      841 catcacttcc aactcctcca aaaatgcatg tgtccgttga aggggatccg gttgacctag
      901 gctttacggt ggcagctttg acagataaat cgatccgttg ggagtacgga ggggtttcat
      961 tcttcctcgc ttcggaaaca ttaactaaag acgagatgat tcaagttgcc tcttctatgg
     1021 tcgcacctga aatgaaatag catcatttga cgtagatgaa cggtcggacg gataataaaa
     1081 agtatcatac agctaagaga gggaaatcat atggaacaga ctacttttta ccgtccgact
     1141 aaagctacgg ttcaactcga tgcaatccgt cagaacgtta agaatcttca acagtattta
     1201 ggggctgaga ctcgtgtcat tgccgttgtt aaagcggatg gctatgggca cggtgaggtt
     1261 gaagttgcaa aagcagcact ggaagccggt gcaattatgg ccgccgtcgc aacacctgat
     1321 gaagctgtga ggctacggaa agggggcgtc aagggcgaca ttctcgtcat gggtccagcc
     1381 cctgcttcat ttgccaaatt tgctgctgaa gaagagatta cggtgaccgc aacgggagtt
     1441 gattggctca cagatgtcct tgcaggtacg ccgctttcaa agtcattaaa gattcatgta
     1501 aaaattgata gtggaatggg cagaatcggg ataagggaag aggaagaatt aaaaacgttc
     1561 tactctcttg caaaggaagc cgaaaatatc atattggatg gtatctttac ccactttgca
     1621 tgtgcagatg aagaaaatcc aacagctaca gaacaacaat tcaaccgttt catggaactg
     1681 gttgatgttc tgccggaaaa accgcgtctg gtacatgcgt ccaattctgc ggcttcgttg
     1741 ctctatccta catatgcatt ggatgcagtt cgctttggtg ttggactcta cggtatcgca
     1801 ccttccgatt atgtagagca aaaccttcca ttcccgcttg ttcgtgcact aacacttaag
     1861 actaaacttg ctttcgtaaa aaagcttccg gccaatagca aaatcagcta cggtggaacg
     1921 tatgaaacag gaggggatga atggatcggt accctgccaa tcgggtatgc ggatggctta
     1981 cggagaggct tgcgcggcca agaagttctc atcgggggag aaagagtccc aattgttggg
     2041 acaatctgta tggatcaatg tatggtcaga ctcccgaagg aaatgccggt cggtgaagaa
     2101 gtcgttctga tcggtcaaca aggcaaggaa gaaataacga tggaagaatg ggcagaccgg
     2161 ttacagacga ttccatatga aattgctgtt tccatggcaa aacgggtgcc cagagagtat
     2221 atcggaaaga cggaataaca gacgcaataa tactccattc gacaaaaaac cgaatcctat
     2281 gcagaaagat gtcaaaaaac taccttctat gtcttctcat tgttacgctt caatggtaag
     2341 ataaacatgt agtgaaatga gggaacgggt ttgtcggagg tgctaatgtt gcgtgctagg
     2401 aaaaacatga aagaaataat agtgaagatt ccgaaacaga tgttgaacga aaatgaacat
     2461 acggtcgtcc atctggagca agaccgcggc gatttcgtct atatttcgac aaagaggtac
     2521 gtgacaaatc acgagccgaa cgtaattagg gaagcgatga tgaaaggata cttggaaatg
     2581 tcccaaatca accttcggat cgccaaggaa tgccttcatg tagaatatga agcgcagcat
     2641 acggtggaac gtctcgtaag cggaggatga aaacttggca ataaaacgtg gggatgtctt
     2701 ttttgcagac ttatcgcctg tcgtcggatc ggagcaaggt ggaacaagac cggtgctcgt
     2761 tatacagaac gacattggaa atcgattcag ccctaccgtc atcgtggcgg cgattactgc
     2821 acagatccag aaggcaaaat tgccgacaca cgttgaaatc gatgcggaac gatacgggtt
     2881 cgagcgagac tcagtcatct tgctcgaaca ag
//
LOCUS       DASULI010000312         4927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_748090, whole
            genome shotgun sequence.
ACCESSION   DASULI010000312 DASULI010000000
VERSION     DASULI010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748090"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /gene="narH"
                     /locus_tag="VFX34_06655"
     CDS             complement(<1..580)
                     /gene="narH"
                     /locus_tag="VFX34_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324613.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX5564622.1"
                     /translation="MRIKAQIAMVMNLDKCIGCHTCSVTCKTAWTNREGAEYMWFNNV
                     ETKPGIGYPKRWEDQELYKGGWHLRNGKLELKSGSKLSKIALGKIFYNPDMPEIKEFY
                     EPWTYDYEKLTMAPESEHTPVARAKSVISGDYMDLEWGPNWEDQLAGAHITGPKDPNI
                     EKIEEEVKFNFEQAFMMYLPRLCEHCLNPSCVA"
     gene            complement(570..4241)
                     /locus_tag="VFX34_06660"
     CDS             complement(570..4241)
                     /locus_tag="VFX34_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX5564623.1"
                     /translation="MNRFSMKYFKPVERYSGNWSVLEEKSRDWENMYRQRWSHDKVVR
                     TTHGVNCTGSCSWKVFVKNGIITWENQQIDYPSCGPDMPEFEPRGCPRGASFSWYEYS
                     PLRVKYPYIRGKLWRMWSRALSEHKDPIKAWASIVEDPAKTAEYKKARGKGGHVRIHW
                     RDVKILIAAQLIYTIQKYGPDRIAGFTPIPAMSMVSYASGARFLSLLGGEMLSFYDWY
                     ADLPPSSPQIWGEQTDVPESSDWFNAGYIIMWGSNVPLTRTPDAHFMTEVRYKGTKTV
                     SVAPDYAESVVHADDWIAPNPGSDAAVAQAMTHVILDEFYEQRKEPMFLDYAKQFTDM
                     PFLITLEEHDNAYKAGRFVRASDLGQESEHAEWKPVIIDGAKNEIIVPNGTMGQRWEK
                     DAKWNLILENEDGTKVDPVLSVIDQGAEWKEIHFPYFDNMGNGVFTRAIPAKKVKFAD
                     GTERLVATVYDVMMSQYGINRSNSKWDAKGYDDAESLYTPAWQQKVTSVKPELVTQIA
                     REFAQNAIDTGGRSMIIMGAGINHWFNSDTIYRAILNLVTLTASQGNNGGGWAHYVGQ
                     EKCRPIEGWSSIAFARDWQAPPRLQNATSFFYFATDQWKYEESGTSALASPLGKQSRY
                     EHPADYNVLAARLGWLPSYPQFNRNSLQLTEEARQAGKTTTEEIVNYTVEQLKTGKLN
                     FAIEDPDAPENFPRSLFVWRSNLVSSSAKGQEYFMKHLFGASSGLIAQPNEDMKPEEI
                     VWRDEVEGKLDLLVALDFRMTATPLYADLVLPAATWYEKVDLSSTDMHPFVHPFNPAV
                     DPLWESRSDWDIFRSIAETFSEMAKNHLPGVYRDLVTTPLAHDSIQEIAQPYGEVKDW
                     KKGELEPIPGKTMPGMTIVERDYTKVYDKYITLGPLLSTGKVGAHGVSFSVAEEYELM
                     KSINGVYTDGTIKDGLPKLYTAKNAAEAILTLSSATNGRVSQRAFESAEQDTGVELKD
                     ISADRAAERFTFANITAQPREVIPTPVFSGSNKLGRRYSPFTTNIERLVPFRTLTGRQ
                     HFYIDHELFMDFGESLPVYKPTLPPMVLGPRDKPIVGGQDALVLRYLTPHGKWNIHST
                     YQDNQHMLTLFRGGPTVWISDLDANEHNIDDNAWLEVYNRNGVVTARAVVSHRMPKGT
                     MFMYHAQDKHIQVPGSEITEERGGSHNAPTRIHMKPTQMVGGYAQLSYGFNYYGPIGN
                     QRDEYVAVRKMKEVNWLEN"
     gene            complement(4452..>4927)
                     /locus_tag="VFX34_06665"
     CDS             complement(4452..>4927)
                     /locus_tag="VFX34_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5564624.1"
                     /translation="GEKTLVAYIQEKYHDGLRDELPMLAPYITKVARVHGENHPHLLR
                     VQEIFKTLRAELLDHTDDEDKNVFPLILEFLTNPTPELKEQLKPHVSELEQEHDNAGR
                     LLHELREITDNFTPPEGACGTYRLVYARLEQLEKETFEHVHLENNVLFERIAKAL"
BASE COUNT         1084 a         1363 c         1144 g         1336 t
ORIGIN      
        1 aggcgacgca gctcgggttc aagcagtgtt cgcaaagcct tggcaagtac atcatgaagg
       61 cttgctcaaa gttgaatttg acctcttctt cgatcttttc gatattcgga tctttcggac
      121 ccgtgatatg agcgccggcc aactggtctt cccagttcgg tccccactcc aagtccatat
      181 aatcaccgga gatgaccgat ttcgcacgtg cgacaggcgt atgctcgctt tcaggagcca
      241 tcgtcaattt ctcgtagtca tatgtccacg gctcgtagaa ttcctttatt tccggcatat
      301 ccgggttgta gaagattttc cctagggcaa ttttcgaaag cttcgaacca gatttcagtt
      361 caagctttcc gttgcgtaag tgccagccgc ctttgtacag ttcctggtct tcccaccgtt
      421 tcgggtaccc gatgcccggc ttcgtttcga cgttattgaa ccacatgtac tcggcgcctt
      481 ccctgttcgt ccatgctgtt ttgcacgtga cactgcacgt atggcagccg atgcatttat
      541 ccaaattcat gaccattgca atttgcgctt taattctcaa gccagttgac ctccttcatc
      601 ttgcggaccg ccacgtattc atcccgttgg ttaccgatcg gcccgtaata attgaatccg
      661 taactcagct gtgcgtaacc gccgaccatc tgtgtcggct tcatatggat ccgggtcggc
      721 gcgttatggc ttcctccccg ttcctccgta atctcggagc ctggaacttg gatatgttta
      781 tcctgcgcgt gatacatgaa catcgtaccc ttaggcatcc tgtgactgac aacggcgcgc
      841 gctgtgacga caccgttccg gttgtacact tcaagccatg cgttgtcgtc gatgttatgc
      901 tcgttcgcgt caaggtcgga aatccataca gtcggaccgc cgcggaacag cgtcagcata
      961 tgttgattgt cctggtaggt cgagtggatg ttccactttc cgtgcggcgt caaatatctg
     1021 aggacaagcg cgtcctgccc tccgacaatc ggcttatccc gcggtcccaa taccatcggc
     1081 ggcaatgtcg gcttatagac cggcaacgat tcgccgaaat ccatgaacaa ctcatggtca
     1141 atatagaaat gctgtcttcc tgtcagcgtc ctaaacggca caagcctttc gatattcgtt
     1201 gtgaatgggg aataccgtct gcccaattta ttcgatccgc tgaaaaccgg cgtcggaatg
     1261 acttccctcg gctgggccgt aatattggcg aaagtgaacc gctcagccgc ccggtctgct
     1321 gaaatatcct tcagctcaac gccggtatcc tgttcagccg attcgaaggc acgttgcgaa
     1381 acgcgaccgt tcgttgcaga tgaaagggtg agtatcgctt cagccgcatt tttcgccgta
     1441 tacaatttcg gcaagccgtc cttgatcgtt ccgtccgtat aaacgccatt gatgctcttc
     1501 atcagttcat actcttccgc caccgagaag gaaacgccgt gcgcgccaac ttttccggtc
     1561 gaaagcaatg gaccgagtgt gatgtatttg tcatacacct tcgtataatc ccgctcgaca
     1621 attgtcatgc caggcattgt cttgccagga atcggctcca gctcgccttt tttccaatcc
     1681 ttcacttctc cgtaaggctg cgcgatttct tgaatggaat cgtgtgcgag aggcgtcgtc
     1741 accaagtccc tgtaaacgcc aggcagatgg tttttcgcca tttcggaaaa cgtttccgcg
     1801 attgaccgga agatatccca gtcggagcgt gattcccata acggatcgac cgcaggattg
     1861 aacgggtgga cgaatggatg catatcggtt gaagacagat cgactttttc gtaccaagtc
     1921 gctgccggca atacaaggtc tgcatataat ggcgtcgcgg tcatccggaa gtcgagcgct
     1981 acgagaagat cgagcttccc ttcaacttca tcccgccaga cgatctcttc cggcttcata
     2041 tcttcgttcg gctgcgcaat caacccggag gaagctccga ataaatgctt catgaaatat
     2101 tcctgacctt tcgcggagct cgatacaagg ttcgaacgcc aaacgaaaag tgatctaggg
     2161 aagttttccg gtgcatccgg atcttcgatg gcaaaattca gtttgccagt tttcaattgt
     2221 tcgactgtat aattgacaat ttcttccgtt gtcgtcttgc ccgcctgcct tgcttcctcc
     2281 gtcaactgca agctgttcct gttgaattgc ggataggatg gaagccatcc gagtcttgcg
     2341 gcaagcacat tgtaatccgc cggatgttca taacgtgatt gcttccctaa cggcgatgcc
     2401 aaagcacttg tgccgctttc ttcatatttc cattggtctg tcgcgaaata gaagaacgat
     2461 gtagcgttct gtaagcgagg aggcgcttgc caatcccttg cgaaagcgat cgagctccat
     2521 ccttcgatcg gacggcattt ttcctgtccg acgtaatgcg cccatccgcc gccattgttt
     2581 ccttgtgacg cagttagggt aactagattt agaatggccc gatagatcgt gtcgctattg
     2641 aaccagtggt taatgcccgc ccccatgatg atcatcgaac gtccgcctgt gtcgattgcg
     2701 ttttgcgcaa actcgcgggc aatctgtgta acaagctccg gtttgacact cgtcactttc
     2761 tgctgccaag caggcgtgta taaggattcc gcatcgtcat agccttttgc atcccacttg
     2821 ctgtttgaac ggttaatgcc gtattgactc atcatgacgt cgtatacagt tgcgacaagt
     2881 cgctctgtcc cgtccgcaaa cttcactttc ttcgccggga tggcacgtgt gaaaacgcca
     2941 ttgcccatat tatcgaagta agggaaatgg atctctttcc attcagcgcc ttgatcgatt
     3001 acagataaga caggatccac tttcgttcca tcctcgtttt caaggatgag attccatttc
     3061 gcatcctttt cccagcgctg ccccattgtc ccgttcggaa cgatgatttc atttttcgcg
     3121 ccatcgatga tgaccggctt ccattccgca tgctcgctct cctggccaag atcactcgcg
     3181 cggacaaaac gccccgcttt gtatgcattg tcatgctctt caagcgtgat taggaacggc
     3241 atatctgtga attgctttgc ataatcgagg aacatcggct ctttccgctg ttcataaaac
     3301 tcatccaaaa tgacatgggt catcgcttga gcgaccgctg catccgatcc gggatttggc
     3361 gcgatccaat cgtccgcatg gacgacgctt tccgcgtagt ccggcgcgac ggaaaccgtc
     3421 ttcgtccctt tatagcggac ttctgtcatg aagtgtgcat ccggcgtccg cgtcaacgga
     3481 acgttcgagc cccacatgat gatatagccc gcattgaacc agtcgctcga ttccggaaca
     3541 tccgtctgct cgccccaaat ttgcggcgag gatgggggta gatccgcgta ccagtcgtag
     3601 aagctaagca tctctccgcc caatagcgat aggaaacgtg ctcccgacgc atagctcacc
     3661 atcgacatcg ccggaatcgg tgtgaagcct gcaatgcggt caggtccgta tttctggatt
     3721 gtgtaaatga gttgggccgc gatgaggatt ttcacatcgc gccaatggat tcgtacatgc
     3781 ccgccttttc cgcgcgcttt tttgtactcg gccgttttcg ccggatcttc tacgatactt
     3841 gcccaagctt tgatagggtc tttatgctcg gaaagcgccc ggctccacat acgccacaat
     3901 ttcccccgta tgtatggata cttcactcgc aacgggctgt attcgtacca cgagaaggat
     3961 gctccccttg ggcagcccct cggttcgaat tcaggcatgt ccggaccgca ggacggataa
     4021 tcgatctgct ggttctccca cgtgataatt ccgtttttca caaacacttt ccagctgcat
     4081 gatcccgtac agttaacacc gtgcgtcgta cgtacaactt tgtcatggga ccatctttgg
     4141 cgatacatat tctcccaatc cctactcttc tcttcaagca cggaccagtt gcccgaatag
     4201 cgctcaaccg gcttgaaata cttcatgcta aacctgttca tggacattga ataaattcac
     4261 tccttttatc agaatgacag acagataggg gaaatcgaca tacctatctt tcctattacc
     4321 cttaatgcta tctactaatc ggaatatttc caattagggt tttccctata tcagccaagg
     4381 gaattcacca attaaaaaag ccgccgcttt ggcagctttt taagcttata tgagttattg
     4441 ctcaattccg attacagcgc ctttgcaatc cgctcgaaca aaacattatt ctcaagatgc
     4501 acatgttcaa acgtctcttt ttcaagctgc tcaagtcttg cgtagacaag ccggtatgtt
     4561 ccgcaagccc cttccggcgg cgtgaagttg tcagtgattt ccctaagctc gtgaagcagt
     4621 cgtcccgcat tgtcatgctc ctgctccaat tcggatacgt gaggcttcaa ttgctccttc
     4681 agttcaggcg taggattagt tagaaactca agaataagcg ggaaaacatt tttatcttca
     4741 tcatcggtat gatcgagaag ttctgcacgc aacgtcttga aaatctcctg cacacgcagc
     4801 aaatgcggat ggttctcgcc atggacacgg gcgaccttcg taatataagg cgcaagcatc
     4861 ggcagttcat cgcgcaggcc atcatggtat ttttcctgga tgtaagcgac caacgtcttc
     4921 tccccga
//
LOCUS       DASULI010000313         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1721461,
            whole genome shotgun sequence.
ACCESSION   DASULI010000313 DASULI010000000
VERSION     DASULI010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721461"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFX34_06670"
     CDS             <1..198
                     /locus_tag="VFX34_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5564625.1"
                     /translation="QVTFEAEFEGTGTNPWGVQVAAFEVEGKISRKEFGLTWNQALEA
                     GGVLVGDDIKISIDLQANPAQ"
     gene            complement(384..1427)
                     /locus_tag="VFX34_06675"
     CDS             complement(384..1427)
                     /locus_tag="VFX34_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5564626.1"
                     /translation="MQKGKWNAITDVPGVKVGHVTLKEEPGPESACTGVTAILPHGGS
                     SFEQKVPAASFVLNGFGKSAGLIQVEELGVIESPIMLTNTFSVGAVLEGTLRHMLEKN
                     TAIGDSTSSLNIVVGECNDSYLNAMRSMKVRPEHAIEAIRNADTGEFDQGDVGAGKGM
                     ICFGRKGGIGSSSRIVLHGEDSFTVGVLTLTNFGNAEECLIDEWFARNGLKLKTAEGD
                     LKPSQEMKSEKPDGSVMIIVATDAPVDSRQLKRLAKRAAIGLGRTGSHIHNGSGDIII
                     AFSNAYTIPHSNEGDDTVSYTLLRDDAKVMNGLFQAVIEATEEAVYQSLMHAETTTGR
                     HGRTVERWPLVEE"
     gene            1653..2024
                     /locus_tag="VFX34_06680"
     CDS             1653..2024
                     /locus_tag="VFX34_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5564627.1"
                     /translation="MKVKDTCEVTVVHEDIVGKVQKGLPDVGGMVQIFKALADETRLK
                     IAYSLTLEEQMCVCDVAAVIGASNATASHHLRYLREHRLAKSERKGKMVYYSLADSHV
                     SDLVKIAHDHAAEREGVPDDN"
     gene            2014..4113
                     /locus_tag="VFX34_06685"
     CDS             2014..4113
                     /locus_tag="VFX34_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019380955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5564628.1"
                     /translation="MTTKTEYRLENLSCANCAMKFENNVKSIPSVTEATVNFGASKLS
                     FIGDATMEELQAAGAFDGIQVVPLTKRQPDPKIPFYRRKENIVTILSLLFVIAGMAFT
                     YRDVPQQALATGLFAAAILIGGTGMFITGVKNLVRLDFDMKTLMTIAIIGAAIIGEWQ
                     EAAVVVFLFAVSEALEAYSMNKARQSIRQLMDIAPPSAVVRRNGKDLELPTEDIRVGD
                     ILLVKPGQKIAMDGVVLSGRSTVNQAAITGESVPVLKQADDEVFAGTLNEEGALEVTV
                     TKLVGDTTIAKIIHLVEDAQAEKAPSQKFVDRFAKYYTPIIIAIAILVAIVPPLFGGD
                     WHTWVYQGLAVLVVGCPCALVVSTPVAIVTAIGNAARQGVLIKGGVYLEEMGRIHAVA
                     FDKTGTLTKGYPEVTDFITENGEKQNILQAVAAVESMSQHPLARAIVDYAKDQGASAA
                     DIRNFQSVTGKGAFGEVEGTLVHVGSMKWASELTELPSEMKKRAESLQQAGKSVMAVL
                     FDKKFQALIAVTDPLRAESADVLRQLKGVGIRHTVMLTGDDERTAEAIAQLAGVTDVR
                     AGLMPEDKLTAVKGLIGQYGRVAMVGDGINDAPALAASSIGIAMGGAGTDAALETADI
                     ALMADDLRQLPYTMKLSRKTLQIIKENIMFALGLKIVALLLIIPGWLTLWIAIFADMG
                     ATLLVVLNSLRLLRIHK"
     gene            4142..>4535
                     /locus_tag="VFX34_06690"
     CDS             4142..>4535
                     /locus_tag="VFX34_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564629.1"
                     /translation="MKLREWTMEEQEQLIHFLTTNTWPFHGNAHPERELIEKTIEDGG
                     YESDEVKTFWVQNEEEDIVGIVKIHDLQDEIPLFDLRIADRYRGFGYGPKALRLITEF
                     VFGMPEEKIRLEGHTRQDNLAMRKTFERA"
BASE COUNT         1138 a         1143 c         1204 g         1050 t
ORIGIN      
        1 caagttacat ttgaagcgga atttgaagga actggaacaa acccatgggg cgttcaagtt
       61 gctgcattcg aagtcgaagg aaaaatttcc cgtaaagagt tcggtcttac atggaaccaa
      121 gcacttgaag cgggcggcgt actcgtaggc gacgacatca agatttcaat cgatctgcaa
      181 gcaaacccgg cgcaataatt aacttataga tagcgaaacg gcacgcatga gatgcgcgcc
      241 gtttttttat ttcaggggac tcatgctccc gcgctcataa attccgttta gcgctcaaaa
      301 ccaccgctcc cgcgctcata aattccattt cgcgctcaaa accgccgctt ctgcgctcat
      361 aaatcgcatt tcgcgctcat tactcattcc tcaacaagcg gccatctctc caccgttctc
      421 ccatgtctcc ccgtcgtcgt ctccgcgtgc atcaatgatt gatacaccgc ttcttccgtc
      481 gcctcgatga ctgcctggaa aagtccgttc atcaccttgg catcgtcgcg cagcaacgta
      541 tagctcaccg tatcgtcacc ttcattcgaa tgcggaatcg tataggcatt cgagaacgca
      601 atgatgatgt cgccgcttcc gttatggata tgggagccgg tacggccaag gccgatcgca
      661 gcacgtttag caagccgctt caactgccgg ctatccaccg gcgcatccgt agcgacgatg
      721 atcatgacgg agccgtcagg tttttcgctt ttcatttcct gtgaaggctt caagtcaccc
      781 tcagctgtct tcagcttcaa tccattccgc gcaaaccatt catcaatcaa gcattcttcc
      841 gcatttccga aattcgtcaa tgtcaggacg ccgactgtga acgaatcctc cccatggagg
      901 acaatcctcg aagaagaacc gatcccgcct ttcctgccga agcagatcat ccctttcccc
      961 gcaccgacat ccccttggtc aaactcaccc gtgtccgcat tccggatcgc ttcaatggca
     1021 tgttccggcc gtactttcat cgaccgcatc gcgttcaaat aactgtcgtt gcattcgccg
     1081 acgacaatgt tcaatgaact cgtggaatcc ccgatggccg tgttcttctc gagcatatgc
     1141 cgcagcgtcc cctcaagaac agccccgacg ctgaatgtat ttgtaagcat gatcggcgac
     1201 tcgatgacac ctagctcctc cacttgaata agtcccgccg atttaccgaa gccgttcaat
     1261 acgaagctag ctgccggcac tttttgttcg aaactacttc ccccgtgggg cagaatggct
     1321 gttactcctg tacaggcgct ttcagggccg ggctcctcct tcaatgtcac atgcccgacc
     1381 ttcacacctg gcacgtccgt tatggcgttc cattttcctt tttgcatgct ccacactccc
     1441 gtttcatttg atgatcaata ttggcgcctc gcaatctgca ttgcaaaaca tggcattata
     1501 ggattcatta tcccattcca tactaaggat aaaaacccct ttattccgaa atgaaataaa
     1561 caatcaaata gttgtttgat tgtacttgca tttggaaagg gccgaacata tactggatta
     1621 taatcaaatg gacatttgaa tgaggtgccg aaatgaaagt gaaagataca tgtgaagtga
     1681 cagttgttca cgaggatata gtcgggaaag ttcaaaaggg gctgccggat gtcggcggca
     1741 tggtgcaaat cttcaaggcg cttgcagatg aaacaagatt gaagatcgcc tattcactca
     1801 cgttggaaga gcagatgtgc gtctgcgatg tggcagcggt catcggcgca tcgaatgcga
     1861 cggcttccca tcatttgcgc tacttgcgtg agcataggct tgcaaaatcg gagcgaaaag
     1921 ggaaaatggt ctattactcc ttggcggata gccatgtgtc tgaccttgtg aaaatcgcgc
     1981 atgaccacgc agcggaaagg gagggcgtgc cggatgacaa ctaaaacgga gtatcgactc
     2041 gagaatctat cttgtgcaaa ttgtgcaatg aaattcgaga acaatgtaaa gagcattccc
     2101 tccgtgacag aagcgaccgt caatttcggc gcatcgaaac tctcattcat aggggacgcg
     2161 acaatggagg agttgcaggc agcaggtgca ttcgacggca ttcaagtcgt gccattgacg
     2221 aaaaggcaac ccgatccgaa aatccccttt taccggagga aggaaaatat tgtcaccatc
     2281 ctgtctctcc ttttcgtcat agcgggaatg gctttcactt atcgggacgt tccgcaacag
     2341 gccttagcta caggcctttt tgcagccgcc atcctgatcg gcggaacggg gatgttcatc
     2401 actggcgtca agaatctagt aagactcgat ttcgatatga aaacgttgat gacgattgcg
     2461 attatcggag cggcaatcat cggtgaatgg caggaagcgg ccgttgtcgt ctttttattc
     2521 gcagtaagtg aagcacttga agcttattcc atgaacaagg cccggcagtc gatccgccaa
     2581 ctcatggaca tcgctccgcc gtccgccgtc gtgcgccgga atggtaaaga tctggagctg
     2641 cctacggaag acatccgggt cggcgatatc cttcttgtga agccggggca gaaaatcgcg
     2701 atggacggcg tcgtgctcag cggacggtcg acagtcaatc aggcagctat cacaggggag
     2761 tcagtcccgg tattgaagca agccgatgat gaagtgtttg cggggacatt gaatgaagaa
     2821 ggcgcgcttg aagtgaccgt gacgaaactc gtcggggata cgacaatcgc taaaatcatc
     2881 catttagtcg aggacgccca agcggaaaag gccccttccc aaaagtttgt cgatcggttt
     2941 gcgaaatact acacgccaat cattatcgcc atcgcgattt tagtcgccat cgtaccgcct
     3001 cttttcggcg gggattggca tacatgggtt taccaaggcc tagcggtgct cgtcgtcggc
     3061 tgtccttgcg cgctcgtcgt gtccacgcct gtcgccatcg tgacggcaat cgggaatgcg
     3121 gcccgccaag gcgtcctcat taaaggcggt gtctatcttg aggagatggg ccgcattcac
     3181 gcagtcgcct tcgacaagac gggtacattg acaaaagggt atccggaagt gacggatttc
     3241 attactgaga acggggagaa gcaaaatatc ctgcaagcgg tggctgcagt tgaatcgatg
     3301 tcccagcatc cgttggcacg ggcgattgtc gattatgcga aagaccaagg tgcaagcgca
     3361 gccgatatcc ggaacttcca gtcggtcacc gggaaagggg ctttcgggga agtggagggg
     3421 actctcgttc atgtcgggag catgaagtgg gcatccgagc tgacggaact gccatcggag
     3481 atgaaaaagc gcgcggagag cttgcaacag gcagggaaat ccgtaatggc ggttctcttc
     3541 gataaaaaat tccaggcatt gattgcagtg acggatccat tgcgggcgga aagcgctgat
     3601 gtattgcgtc aattgaaggg agtcggcatc cggcatacgg tcatgctgac gggagatgac
     3661 gagcggacgg cagaagcgat cgcgcaactt gccggcgtga cggatgtccg tgcagggctt
     3721 atgccggaag ataaattgac ggccgtgaaa ggattgatcg gtcaatatgg ccgggtcgcg
     3781 atggtcggcg acggcatcaa tgacgcgccg gcgcttgccg cttcttcaat agggatcgcc
     3841 atgggaggag ccgggacgga cgccgcgctc gaaacggcgg atatcgcctt gatggcggat
     3901 gatctccgtc aattgccgta tacgatgaaa ttaagccgaa aaacgttgca gattattaaa
     3961 gaaaatatta tgtttgcatt aggcttaaaa attgtagcgc tgcttttgat catcccagga
     4021 tggttgacct tatggatcgc aattttcgcg gatatgggtg cgacattgct cgtcgtgctg
     4081 aattcattga gattgctgcg gatccacaag tgaagtacgc agcccgaacg ggggaattga
     4141 aatgaaattg cgggaatgga caatggaaga acaagaacag ctcatccatt ttttaacgac
     4201 gaatacatgg ccgttccatg gaaacgcaca tccggaacgt gaactgatcg aaaagacgat
     4261 tgaagacggc gggtatgaat cggatgaagt gaagacgttc tgggtgcaaa acgaggaaga
     4321 ggatatcgtc ggaatcgtga agatccatga tttgcaggat gaaattccgc ttttcgattt
     4381 acggattgca gaccgttatc ggggattcgg gtacggcccc aaagcactcc gccttataac
     4441 ggagttcgtg ttcggcatgc cggaggagaa aatccgtctg gaaggccata cgcgacaaga
     4501 caatttagcg atgcggaaga cattcgagcg agccg
//
LOCUS       DASULI010000314         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_515034, whole
            genome shotgun sequence.
ACCESSION   DASULI010000314 DASULI010000000
VERSION     DASULI010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515034"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFX34_06695"
     CDS             <1..536
                     /locus_tag="VFX34_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006207208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5564630.1"
                     /translation="KGDIDMRVMRRFIGGSFIGLPAGIIIFLLVDIPKLKLGIGVVIL
                     VLTALLLLKLKIKQTPNRDVAIGGLSGSLTTSIGMPGPPLLLYFSGTDTAKETLRGTT
                     LVYYLFIYSISLLIQIIFAGTSMAVWKSSLLALPLVWLGLYAGQYLFLKVNQNGFRIF
                     MYIILLFTGLYLLVDSI"
     gene            complement(595..1578)
                     /gene="guaC"
                     /locus_tag="VFX34_06700"
     CDS             complement(595..1578)
                     /gene="guaC"
                     /locus_tag="VFX34_06700"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0015950 - purine nucleotide interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEX5564631.1"
                     /translation="MDTVFDYEDIQLIPAKCIVNSRSECDTSVTLGKHKFVLPVVPAN
                     MQTIIDEKIAVQLAEGGYFYVMHRFNPETRAAFIKDMHTRGLIASISVGVKEEEYGFV
                     EQLAADQLIPDYITIDIAHGHSNAVIRMIQHIKQHLPETFVIAGNVGTPEAVRELENA
                     GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAK
                     SVRFGASMVMIGSLFAGHEESPGETIEVDGKLYKEYFGSASEFQKGEKKNVEGKKMHV
                     EYKGRLQDTLTEMQQDLQSSISYAGGTKLDAIRTVDYVIVKNSIFNGDKVY"
     gene            1782..>2194
                     /locus_tag="VFX34_06705"
     CDS             1782..>2194
                     /locus_tag="VFX34_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5564632.1"
                     /translation="MRLSLKYLIGLVALLSVVLTLAASIISGYRAEQQSLITNTLETN
                     RAYAVKMAQSTESYLEMTLQSMEASAQQVAPFIGQESAEQTLLSEAERMKKQTNTFNS
                     VSIVDKDGEILAVSPETLSLKGKKLNSEGGREALT"
BASE COUNT          547 a          486 c          528 g          633 t
ORIGIN      
        1 taaaagggga tattgatatg agggtcatga ggcggtttat cggagggagc tttatcgggc
       61 tgccggcggg aatcattata ttcctgcttg tcgacattcc gaagttgaaa ctggggattg
      121 gcgttgtcat tcttgtattg accgcgttat tgcttctgaa gctgaagatc aagcaaacac
      181 cgaacaggga cgttgccatc ggcgggcttt ccggctcatt gacgacgagc atcggtatgc
      241 cgggtccgcc gttgctgctc tatttttcgg gcacggacac agcaaaggag acattgcgcg
      301 gcacgacgct cgtctattat ctgttcatct attccatcag tttgcttatc cagatcattt
      361 tcgcgggtac aagcatggcc gtatggaaat caagtcttct cgcgctgccg ctcgtttggc
      421 ttggcctcta cgcgggtcag tatttattcc tgaaggtcaa tcaaaacggc ttccgcattt
      481 tcatgtatat catcttactg ttcacgggcc tctatctgtt ggtggacagc atttaattga
      541 aaaaagccac tgaaaatcag gacaaatcct gaatcggtgg cttcatttta tttattaata
      601 aaccttatcc ccattaaaga tggagttttt cacgattaca taatcgaccg tacggatggc
      661 atccaactta gttccgcccg cataggagat ggacgattgg agatcctgct gcatttcagt
      721 taatgtgtct tgcaacctgc ctttgtattc cacatgcatt ttcttgcctt cgacgttttt
      781 cttctcgcct ttttggaatt ccgaagcaga tccgaaatac tccttataca gcttgccatc
      841 cacttcgatc gtttcacctg gggactcctc gtgtccagcg aaaagcgatc cgatcatgac
      901 catggaagcg ccgaatcgga ctgatttcgc gatgtcgcca tgtgtgcgga ttcccccgtc
      961 agcgatgatt ggctttgaag cggcttttgc gcaccagcga agcgcggcta actgccatcc
     1021 gccagtaccg aaaccggttt tgattttcgt gatgcaaact ttccctggtc cgatgcctac
     1081 ttttgttgcg tccgctccag cattttcgag ttcgcgcacc gcttccggcg tcccgacgtt
     1141 gccggcgatg acaaatgttt ccggaagatg ttgtttgata tgctggatca tacggattac
     1201 cgcgtttgaa tgtccgtgcg caatgtcgat tgtaatgtaa tcggggatga gctgatcggc
     1261 agccaactgc tcgacaaaac cgtactcttc ctctttcaca ccgacactga ttgaagcaat
     1321 caaaccacgg gtatgcatat ctttaatgaa agctgcgcga gtttcggggt tgaaacggtg
     1381 catgacatag aaataaccgc cttcagccaa ctggacggcg attttttcat cgatgatcgt
     1441 ctgcatattt gcagggacga ccggtaatac gaatttatgt ttacctaacg taacggaagt
     1501 gtcacattct gagcggctat tcacaataca tttagcggga atcaattgaa tatcttcata
     1561 atcaaatact gtatccatac attacactcc taaaggcgaa tgatattttt tatttcgatg
     1621 ataatgttcg tacttttgta atttacatca tttttccttg ttcgtcaatg aaatagtatc
     1681 tctataaaaa gcaaattata ttccaatata agggtgttca acgggtgtgt aggtgatatg
     1741 atgggtgatg gtttgaaaat ttatagagga gatcggttgc attgaggctg tctttaaagt
     1801 atttaatcgg gctggtggcg ttattatcag ttgtgctgac gttggcggcc agcattattt
     1861 caggttatcg ggctgagcag caatcattaa ttacaaatac attggaaacg aaccgtgcat
     1921 atgcagtgaa gatggcgcag tcaacggagt cgtatttgga gatgacgctg caatccatgg
     1981 aagcgagtgc gcaacaggtt gcgcctttca tcggtcaaga aagtgcggaa caaacgttac
     2041 tttccgaagc ggaacgaatg aaaaaacaga caaacacatt caattccgtt tcaattgtcg
     2101 ataaagatgg agagattttg gctgtctccc cggagacgtt gtcgttaaaa ggcaaaaaac
     2161 tgaactcgga aggcggtcgt gaggcgctga cgaa
//
LOCUS       DASULI010000315         8431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_395810, whole
            genome shotgun sequence.
ACCESSION   DASULI010000315 DASULI010000000
VERSION     DASULI010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395810"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFX34_06710"
     CDS             complement(<1..517)
                     /locus_tag="VFX34_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5564633.1"
                     /translation="MTNAKKRIWNGNVRDAALSILMEINQNQAYSNLLLNRTIKKYAI
                     DPKDRGLLTELTYGTLQHRLTLDYYLEPFVKGKLDPWVRELLRLSIYQIVYLTKIPPH
                     AVVHEAVEIAKRRGHKGISSTVNGILRSVLRKGVRPIEEITDDIERMSVETSRPVWLI
                     ERWVKQFGKEEA"
     gene            complement(514..1455)
                     /gene="fmt"
                     /locus_tag="VFX34_06715"
     CDS             complement(514..1455)
                     /gene="fmt"
                     /locus_tag="VFX34_06715"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5564634.1"
                     /translation="MTKIVFMGTPDFSVPVLTMLHEEGHDILAVVTQPDRPVGRKRVL
                     TPPPVKEEAIRLGLPVLQPEKLRTSEELDKLIQMKPDLIVTAAYGQILPKNLLDAPRL
                     GCINVHASLLPKYRGGAPIHQAVIDGEKETGVTIMYMVEKLDAGDIISQTTVPITDDD
                     NTGILFEKLSVAGTELLKETLPSIIDGTNERIPQDEKLVSFAGNITREQERIDWSKGG
                     TAIFNQIRGLYPWPGAYSVFQGENVKIWKAEKSDIHSDVTPGMIIKLMKDRLIIKTGN
                     ETSIAITELQPAGKKRLSAEEFLNGVGAKWNEGEQFE"
     gene            complement(1452..1949)
                     /gene="def"
                     /locus_tag="VFX34_06720"
     CDS             complement(1452..1949)
                     /gene="def"
                     /locus_tag="VFX34_06720"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019416033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5564635.1"
                     /translation="MVIRKIVESPSTLLTQRCKEVEKFDGKLAKLLDDMYDTMIDADG
                     VGLAAPQVGELVRVAIVDMGEGNHVIEMVNPVVTAIGGSVVEVEGCLSFPGLYGEVER
                     PFFVRVEAQERDGSLYELEAEDYEARAILHEIDHLDGILFDSKITRIVDPEEFEEVDE
                     EGEVE"
     gene            complement(1983..4394)
                     /gene="priA"
                     /locus_tag="VFX34_06725"
     CDS             complement(1983..4394)
                     /gene="priA"
                     /locus_tag="VFX34_06725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5564636.1"
                     /translation="MIAEVIVDVSAYPIDRPFDYAVPAHLETVIERGSRVKVPFGPRK
                     VLGYVTGLKSESEIEEHKLKKIDELIDLDPVLSPELMDLAKKMARETLSYEIDALQVM
                     LPAAMRAKYEKFISIEKPDEIDDARVLAVLNGRTRIPLKQITDSAVLKLIKDYSERGI
                     VSVDTAISQQTEVKKVRVVQIGDSSTLAELLLTIHPNAKKQAELIRWMSDHAGETIEA
                     AKLLNESGIQNPVLKAVIEKGGATEAYVETYREPDAPKLLDTPIPIRLTEEQLTALDK
                     VKTAADSKKAETFLLHGVTGSGKTEVYLQAIKHVLEEGKEAIVLVPEISLTPQMTARF
                     QERFGGLVAVMHSGLSSGEKYDEWRKIHRREVKVVVGARSAIFAPFENVGIIILDEEH
                     ESTYKQEDTPRYHARDVAIWRSEHYGCPVILGSATPSLESYARASKGVYTLLELSKRA
                     KNQPLPSVTVVDMRDELKEGNRSMFSVQLAEAIRLRLEKKEQTVLFLNKRGFSSFVLC
                     RDCGTVVQCPNCDISLTYHRSNESLKCHYCGHEEPVPLVCPECESEHIRFFGTGTQKA
                     EEEISKLFPEARVLRMDVDTTRQKGAHERILRQFSEGKADILLGTQMIAKGLDFPNIT
                     LVGVLAADTTLHLADFRAAEKTFQLMTQVSGRAGRHDLAGEVFIQTYSPDHYAIELAK
                     TQHYETFYNLEMSARKQYGYPPFYFITLVQFSHEDLLKVADFAEKGTRYLKNSLSPET
                     VIIGPAAAAISRVNNRYRYQCLIKYKKEPKLIETLQQLIRMYRTSWMKEGLLMTVDMD
                     PTSIF"
     gene            complement(4384..5601)
                     /gene="coaBC"
                     /locus_tag="VFX34_06730"
     CDS             complement(4384..5601)
                     /gene="coaBC"
                     /locus_tag="VFX34_06730"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5564637.1"
                     /translation="MLLNKKILVCVTGGIAVYKAVALVSKLAQAGVEVKVMMTASAME
                     FVTPLSFQVMSRNDVYFDTFDEKDSSVIAHIDLADWADLIIVAPATANVIGKLANGIA
                     DNMVTTTLLATTAEVWIAPAMNVHMYENKSVIRNIEQLHEDGYRFIEPSEGFLACGYV
                     GKGRLEEPEKIVQLINERFSQPEILPLSGKKVVVTAGPTRERIDPVRYISNFSSGKMG
                     YSLAEAAVALGAETILVSGPVDLPEPQGVQLIKVESAAEMLDAVSSQFGDADLVIKSA
                     AVADYRPKEQHSQKMKKKDGDSSIALERTTDILKSLGERKERQILVGFAAETNDAIEY
                     GMKKLSNKNLDYIIVNDVTDPDGGFGSDTNVVTLLSRKGKTYPFGAMHKKELAKVLLR
                     TILELESDNSNDR"
     gene            complement(5738..5962)
                     /gene="rpoZ"
                     /locus_tag="VFX34_06735"
     CDS             complement(5738..5962)
                     /gene="rpoZ"
                     /locus_tag="VFX34_06735"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5564638.1"
                     /translation="MLYPSVDSLKGKIDSKYTLVTLAAMRAREMQESKNLLLHSYKSH
                     KNVGKALEEVAAGALSKQVQDASIVYEDEV"
     gene            complement(5966..6583)
                     /gene="gmk"
                     /locus_tag="VFX34_06740"
     CDS             complement(5966..6583)
                     /gene="gmk"
                     /locus_tag="VFX34_06740"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5564639.1"
                     /translation="MYKQRGLLIVLSGPSGVGKGTVRKELFQQPDTNYEYSISMTTRS
                     PREGEQDGVDYFFKSRSEFEQLISEGKLLEYAEYVGNYYGTPLDYVNETLDAGRDVFL
                     EIEVVGAAQVRKLVPDGLFIFLAPPSLSELEDRLVGRGTEKADVIASRVLKAREELEM
                     MNLYDYVVENDEVSKACDRINAIVTAEHCRRERVEKRYLLMLEGE"
     gene            6715..8406
                     /locus_tag="VFX34_06745"
     CDS             6715..8406
                     /locus_tag="VFX34_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEX5564640.1"
                     /translation="MAFDGLFTAAITQELQQIKDGRISRIHQPNAQEVVLLVRAGRTN
                     YKLLISAHPSYSRVQLTDETIANPAEPPMFCMVLRKHLEGGMIQSIRQSGNDRIISFD
                     IRAKNEIGDDIHRRLIVEIMGRHSNLLLIDPERDLIIDSMKHLPPSVNSYRTVLPGQP
                     YVPAPPQEKLNPFELDENKFLALLPEWENAKQVVGKLSGFSPIHGEELLHRLKEEAEG
                     DAYGVFKSFLSSFNGGAHTPTIAEVGNKTIFSATSLTYADKTVATFDTLGELLDKVYF
                     ARAERERVKSQAADLERWLDNEIAKLKLKMEKLEKEQKAAEKLDTFQLYGELLTANSY
                     AINKGDTEAVVDNYYEEGTTVTIPLDPRKTPIENAQHYYSRYTKAKNALIMIAEQLTK
                     AKEDIDYFEMIKQQVMQASPEDIDEIREELADLGLMRARRGKKKQRPKKPSPESYISS
                     SGIPISVGKNNKQNDYLTFKIAARDHVWLHTKDIPGSHVVIHDTDPDERTIEEAAVLS
                     AYFSKARGSSSVPVDFTEIRHVKKPNGAKPGFVIYFEQKTLFVTPDEDVVRKLRK"
BASE COUNT         2145 a         2172 c         1817 g         2297 t
ORIGIN      
        1 aagcttcttc cttgccgaat tgcttcaccc aacgttcaat caaccacaca ggacgacttg
       61 tttcaacgga cattctttcg atgtcgtccg taatctcctc gattgggcgt acgcctttgc
      121 gcaatacgga gcgtaaaatg ccgtttacag ttgaagagat tcctttatgc cctcttcttt
      181 tggcgatttc cactgcctca tggacgactg cgtgcggagg gatcttcgtc aaatagacaa
      241 tttggtaaat ggataagcgc aataattccc gtacccaagg atccaacttg cctttcacga
      301 aaggctccag gtaataatcc aatgtcagcc gatgctgcag agtgccgtat gtcaattcag
      361 tcagcaaacc cctgtccttc ggatctattg catatttctt tatcgtccgg ttaagcagca
      421 gattgctgta ggcctgattc tgattgatct ccatcaatat cgataatgcg gcatcgcgga
      481 cattgccgtt ccaaatcctc tttttggcat ttgtcattcg aattgctccc cttcattcca
      541 tttggcgcct acgccgttaa ggaattcttc tgccgacagc cgctttttac ctgcaggctg
      601 caactcagtg atagcaattg aagtctcatt ccctgttttg atgatcagcc tgtccttcat
      661 caatttaata atcatgcctg gcgtaacgtc tgaatggata tcggattttt ccgctttcca
      721 aattttcacg ttctcccctt ggaatacgga atacgctcca ggccacggat agagcccgcg
      781 gatttgattg aaaatagcag ttccgccttt ggaccagtcg attctttcct gttcacgtgt
      841 aatatttcca gcaaatgaaa cgagtttttc gtcttgtgga atccgttcat tcgtaccgtc
      901 aatgatggat ggcaacgtct ctttcaataa ttcggtgcct gcgacggaca acttttcaaa
      961 aagaatgcct gtattatcat catctgtaat cgggactgtt gtctgtgaaa taatatcgcc
     1021 cgcatcgagc ttctcgacca tatacatgat tgtaactccc gtttccttct caccatctat
     1081 gaccgcttgg tgaatcgggg cgcctccccg gtatttcggc aataaggaag cgtggacatt
     1141 gatgcagccg agacgcgggg catccagtaa attcttcggc aaaatttgtc catatgccgc
     1201 tgtgacaatc aaatccggct tcatttggat gagtttatcg agttcttcgg atgttctaag
     1261 tttttcaggc tgcaataccg gcaatccaag tcggatcgcc tcttccttta caggcggagg
     1321 cgtcagtaca cgtttcctcc cgacgggtcg atcgggctgg gtgacgactg caagaatatc
     1381 gtgcccttcc tcatgcagca ttgtcaaaac aggaacggaa aagtccggag tccccatgaa
     1441 tacgattttc gtcattccac ttcgccctcc tcgtcaactt cttcaaattc ctccggatct
     1501 acaatccgtg taattttcga atcaaataaa attccatcca aatgatcgat ttcgtgtaaa
     1561 attgcccgcg cctcgtaatc ttccgcttcc agctcataca aggaaccgtc ccgttcttgc
     1621 gcttcaactc gtacgaaaaa cggccgttcc acttccccgt agagaccagg gaagcttaag
     1681 caaccttcca cttcaacgac agaccctccg attgctgtga cgacggggtt gaccatttca
     1741 atgacgtgat tcccttcccc catatctacg atggcgacac ggaccaactc cccgacttga
     1801 ggagcggcaa gccctacgcc gtccgcatcg atcatcgtgt cgtacatgtc atcgagcaac
     1861 ttagccaatt taccatcaaa cttttccact tccttgcaac gctgagtcag cagtgtcgaa
     1921 ggactttcaa caattttacg tattaccaat tcaattcctc atttccaact cgtccgaacc
     1981 ggttaaaaaa ttgaagtcgg atccatatcc actgtcatca gcagaccttc cttcatccag
     2041 ctcgtccgat acattctgat caattgctga agcgtttcaa tcagcttcgg ctcttttttg
     2101 tattttatca aacattgata acgatatcta ttgttcacac gacttatcgc ggctgccgca
     2161 ggaccgataa tgacggtttc tggagatagt gagtttttca aatagcgggt tcccttttcc
     2221 gcaaaatcgg cgactttcaa taaatcctca tgtgaaaact gtacaagtgt tataaagtag
     2281 aacggtggat atccatattg cttgcgtgca gacatctcca aattgtaaaa cgtttcgtaa
     2341 tgctgggtct tagcaagttc aatcgcgtaa tggtcaggtg aataggtttg aatgaaaact
     2401 tcacccgcca aatcatgcct gccggcccgt ccgcttactt gcgtcatcaa ttggaatgtc
     2461 ttttccgccg cacggaagtc cgccaaatgt aatgtcgtat ccgcagccaa cacccctacc
     2521 aacgtaatat tcgggaagtc gagccctttt gcgatcattt gagtgccaag gaggatatcc
     2581 gccttccctt cactgaattg gcgaagaatc cgttcatgcg cccctttttg acgggttgtg
     2641 tccacatcca tccggagcac ccgagcttca gggaatagtt tcgaaatttc ttcttccgct
     2701 ttttgggtcc ccgttccgaa aaatcggata tgctcgcttt cacattcagg acaaacgagc
     2761 ggcaccggct cttcgtgtcc gcaataatga catttcaagc tttcattcga gcgatgatac
     2821 gtcaacgaaa tatcacagtt cgggcactga acgaccgtac cgcaatccct gcacaaaacg
     2881 aatgatgaga agcctcgttt attgagaaaa aggacggtct gctctttctt ctccaagcgc
     2941 agacggatcg cctccgccaa ttggacagaa aacatcgacc ggttcccttc cttcaattca
     3001 tccctcatat caacgacggt gacggacggc aaaggctggt tcttcgccct cttagataac
     3061 tccagcaatg tataaacgcc ttttgacgcc cgggcatacg attcaagcga aggggtggcg
     3121 cttccgagga tgaccgggca gccataatgc tccgaacgcc agatggcgac atcccttgca
     3181 tgataccgcg gtgtgtcctc ctgtttatac gtcgattcat gctcttcatc caggatgatg
     3241 atgcccacat tttcaaatgg ggcaaaaatg gcggatcttg caccgacgac aaccttcact
     3301 tcccggcggt ggatctttct ccattcgtcg tatttttctc cggacgacaa accgctatgc
     3361 atgactgcga caagaccgcc gaaacgttcc tggaatcggg cagtcatttg aggggtcaat
     3421 gaaatttcgg gaacgaggac gatagcttcc ttcccttctt ccaatacatg cttgatcgct
     3481 tgcagataga cttccgtttt gccacttccc gtgacaccat gcaacaggaa agtttccgcc
     3541 tttttggaat cggccgccgt tttcactttg tcgagggcgg ttagctgttc ttcagtcagc
     3601 cgaatcggga tgggtgtatc aagaagcttc ggtgcatccg gctcgcggta cgtttcgaca
     3661 tacgcttccg ttgctcctcc cttttcaatg acggctttca gcaccggatt ttggatgccg
     3721 gattcattca acaatttagc agcctctatc gtctcccctg catgatccga catccaccgg
     3781 atcaattccg cctgtttctt cgcattcgga tggatcgtga gaagtagctc ggccaaagtg
     3841 gaggaatctc caatttggac aactcgtact ttcttcactt cggtttgctg gctgattgcc
     3901 gtatctacag aaacaatccc tctttccgaa tagtccttta tcaatttcag aacggctgaa
     3961 tcggtgatct gcttcagcgg gatgcgggtc cggccattca atactgccaa aacccttgca
     4021 tcatctattt catcaggttt ttcgatactg atgaatttct catattttgc acgcattgct
     4081 gccggcagca tcacttgcaa cgcatcgatt tcataagata gcgtctccct cgccattttt
     4141 ttagccaagt ccatcagttc aggagaaaga acgggatcca aatcgatcaa ttcatcaatc
     4201 ttcttcaatt tatgctcttc gatttcgctt tccgatttca ggccagtgac gtagccaagc
     4261 acctttctcg gtccaaacgg gactttgacc cgggaaccgc gttcaatcac cgtttccaag
     4321 tgggcgggca cggcataatc aaagggacgg tcgatcggat acgccgacac atcgacaatg
     4381 acttcagcga tcatttgaat tgtcactctc caattccaaa atcgttcgta agagcacttt
     4441 tgccaattcc tttttatgca tcgccccaaa aggataggtt ttcccctttc tggataatag
     4501 tgtgacaaca ttcgtatcgc tgccgaagcc gccatccggg tcggtcacat cgttgacaat
     4561 gatatagtcc aagttcttat tggatagctt tttcatgcca tactcgatgg catcatttgt
     4621 ttccgccgca aacccgacga ggatctgccg ttcttttctt tcaccgagcg atttgaggat
     4681 atcggtcgtt cgctcaaggg caatggaaga atctccatct tttttcttca tcttttgtga
     4741 atgctgctct tttgggcggt aatccgctac agccgccgat ttgatcacta gatctgcatc
     4801 gccgaattga cttgatacgg cgtcaagcat ttctgcagca ctttcgactt tgattagttg
     4861 aacgccttgt ggctccggca agtctaccgg acccgacact aaaattgttt ccgctcctaa
     4921 tgcaacagct gcttccgcga gtgaataccc catcttgccg ctcgagaaat tggaaatata
     4981 acgtaccgga tcgatgcgtt cccgagtagg ccccgccgta actacgacct ttttccctga
     5041 caaaggaaga atttccggtt gagaaaatct ctcattgatc aattggacaa tcttttccgg
     5101 ctcctcgagt ctccctttgc cgacatagcc gcatgccaaa aaaccttccg aaggctctat
     5161 gaaacggtag ccatcctcat gaagctgttc gatgttccgg atgaccgact tgttttcata
     5221 catatgtaca ttcatggccg gtgctatcca aacttctgca gtcgtcgcaa gaagtgtagt
     5281 cgtcaccata ttatccgcaa tgccgtttgc aagcttgcca atgacgttcg ctgttgccgg
     5341 tgcgacgatg ataagatcag cccagtcggc tagatcaata tgcgcaatga cgcttgagtc
     5401 tttttcatcg aacgtatcga aatagacatc attccgtgac atgacttgaa aagaaagcgg
     5461 tgtcacgaac tccattgccg aagccgtcat catcactttc acttcaaccc ctgcttgggc
     5521 aagcttgctc actaacgcaa cggctttata aacggcaatt ccaccggtta cacaaacgag
     5581 tatcttttta ttcaacaaca ttcccaccct ttctaatcaa ctatatgtaa atggttgtcc
     5641 tagaaaacgc ttttataaaa tgacaaactc ccaaagaaaa tctgtttcta agggagtatg
     5701 cagacctatt caaatacctc aacttaatga atgttcatca cacttcgtct tcgtacacaa
     5761 tcgaagcatc ctgtacttgt ttcgataacg cgccagcagc gacttcttcc aaagctttcc
     5821 ccacgttctt atgtgacttg taggagtgaa gcaataaatt cttgctctcc tgcatttcac
     5881 gcgcacgcat agctgcgagc gttacgagtg tgtatttcga atcaattttg cctttaagcg
     5941 agtcaacaga tggatataac atgatttatt ccccttccaa cataagtaaa tatctttttt
     6001 ccacacgttc ccggcggcaa tgctctgccg tcacgatggc attgatgcga tcgcatgcct
     6061 tcgaaacttc atcattttca acaacatagt catacaagtt catcatttcc agctcttcac
     6121 gcgctttcaa gactctgctt gcaatgacat cggctttctc cgttcctctg ccgaccaacc
     6181 gatcttcaag ctcggacaga ctcggtgggg caaggaaaat gaataaccca tcgggaacta
     6241 atttacggac ttgtgcagcg ccgacaactt caatttccag gaatacgtcc cttccggcat
     6301 ccagcgtctc gttcacataa tccagaggag tgccgtaata gttgccgaca tactctgcat
     6361 actccaataa ttttccttca cttatcaatt gctcaaactc gctgcgcgac ttgaaaaaat
     6421 aatcgacacc gtcctgttcg ccttcacgcg ggcttctcgt cgtcatcgat atcgaatatt
     6481 catagttcgt gtcaggttgt tgaaaaagct cttttctgac cgtccctttt ccgacaccgg
     6541 atggtccgga caggacaatc aacagtccac gttgtttgta catgcaatcc ctcttcttat
     6601 gtattctcta gcttttattg ttccattcat catagcaaac aatcttctca tattataaca
     6661 tatcgcaccc agagagtgct aaaatggaac aaaacgtttg aaagaggaat catcatggca
     6721 tttgacggtt tattcactgc agcaattact caagagctgc aacaaatcaa ggacggaaga
     6781 atttcgagaa tccatcagcc gaatgcacag gaagtcgtct tattggtgcg ggccggcaga
     6841 accaattaca aacttctcat ttccgcccat ccatcgtatt cacgcgttca actgactgat
     6901 gaaacgattg caaaccctgc ggaaccaccg atgttctgca tggtattgag gaagcatcta
     6961 gaaggcggaa tgatccaatc gatccggcaa tcggggaatg atcggatcat ctcattcgat
     7021 attcgcgcca aaaatgaaat cggagatgat atccaccgcc gattgatcgt agaaatcatg
     7081 ggaaggcata gtaatttgct gctgattgat ccggaacgcg atctgatcat cgacagcatg
     7141 aagcatttgc ctccttccgt gaacagttac cgtacggttc ttcctggaca gccgtatgtc
     7201 cctgctcctc cacaagagaa attgaaccct tttgagttgg atgaaaataa gttcctagca
     7261 ttattgccag aatgggagaa tgccaaacag gtcgtcggca aactatcggg gttctccccg
     7321 attcatgggg aggagctttt acaccggttg aaggaagagg ctgaaggaga tgcctacggt
     7381 gttttcaagt ccttcctctc gtccttcaat ggcggagcac atacaccgac gatagcggaa
     7441 gtagggaata aaacgatatt ttctgcgaca tctctgacgt atgcagacaa aacggttgcg
     7501 acgttcgata cactcggcga attgctcgat aaggtctatt tcgcacgggc ggaacgggag
     7561 cgggttaaat cccaggcagc cgacttggaa cgctggctcg ataacgagat tgctaaattg
     7621 aagctgaaga tggaaaagct ggagaaagaa cagaaagcgg cagaaaaatt ggacacgttc
     7681 cagctatatg gcgaattgct tactgccaat agctatgcaa tcaataaagg ggatacggaa
     7741 gcagtcgtcg acaactacta tgaggaaggc acgactgtca ccatcccgct cgatccaagg
     7801 aaaacgccga ttgaaaatgc gcagcactat tattcccgct atacgaaggc gaaaaatgcg
     7861 ttgatcatga ttgcagaaca attgacgaaa gcgaaagagg acatcgacta ttttgagatg
     7921 atcaagcagc aggtcatgca ggcttctccc gaggatattg atgaaataag agaagagctg
     7981 gcagatttag gcttgatgcg ggcgagaaga gggaagaaaa aacaaaggcc gaaaaagcct
     8041 tcacccgagt cgtatatttc ttcttccggc attccgattt ccgtcgggaa aaataataaa
     8101 cagaatgatt atttgacatt caagatagcg gcaagagatc atgtatggct tcacacgaag
     8161 gatattccgg ggtcgcatgt cgtcatccat gatacagatc ccgacgagcg gacgattgaa
     8221 gaggcggcgg ttctttcggc ttatttcagt aaggcaaggg ggtcctcatc ggttcctgtc
     8281 gactttacgg aaatccgcca tgtgaagaag cccaatggtg cgaagcccgg ctttgtcatt
     8341 tattttgagc agaagacgtt gtttgtgacg ccagatgaag atgtcgtacg caaactaaga
     8401 aaataataga aggactgtcg gaaagtcgat t
//
LOCUS       DASULI010000316         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1484718,
            whole genome shotgun sequence.
ACCESSION   DASULI010000316 DASULI010000000
VERSION     DASULI010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484718"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VFX34_06750"
     CDS             complement(<1..1535)
                     /locus_tag="VFX34_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5564641.1"
                     /translation="MKCQHCGKNEATMSFRLQVNQQRMQINLCHTCFQEVQGQMKLPN
                     FGMNANQFFQANGNPQAHTQVREVDEHQGNGLLDQLGKNISNDAKEGQIDPVIGRDQE
                     VKRVIETLNRRNKNNPVLIGEPGVGKTAIAEGLAVKIHEGDVPAKLLNKQVYVLDVAS
                     LVTNTGIRGQFEERMKQLIEEIESRKDVIVFIDEIHLLVGAGSAEGSKMDAGNILKPA
                     LARGSMQLIGATTLKEYRQIEKDAALERRFQPIIVKEPSAEDAITILNGIKDRYEAFH
                     EVRYSDEAVRAFVTLSQRYIQDRFLPDKAIDLMDEVGSRMNLKHSSADAESIGNRLEE
                     VVQQKEQAAEMEDYEKAATLRQQEMQLRQQLEEAEQGEASLYEVTVEDIEEIVEEKTG
                     IPVTKLQASEQAKMKDLAESLRKKVIGQEEAADKVAKAIRRSRAGLKSKTRPIGSFLF
                     VGPTGVGKTEITKVLAEELFGSRDTLIRLDMSEYMEKHAVSKIIGSPPGYVGHEEAGQ
                     LTE"
     gene            1721..2452
                     /locus_tag="VFX34_06755"
     CDS             1721..2452
                     /locus_tag="VFX34_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX5564642.1"
                     /translation="MNNSLVSAGAGHPRIVAFAMGDVTGDRVIDQIYLTGTKTPDSPF
                     IQTITLHVRDGRTGIMTSVALHNNVGYNPRLFLGDFTGDGIDDILIAIDTGGSGGIVN
                     YYIFSYVGNQARLLFDSDVYAHHFQYDVTYQDNYKVEVVSRPNNERYIIDISTRDADY
                     LNEIYDANGKLKSPIAGFVDPLSGLYPVDFDANGVYELLGYQKISGRYHADSLGYIMN
                     TLEWKNNGFLLREQYLAIFGSQLQQ"
     gene            complement(2508..3746)
                     /locus_tag="VFX34_06760"
     CDS             complement(2508..3746)
                     /locus_tag="VFX34_06760"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX5564643.1"
                     /translation="MKLERLRQSFEELARFTEKGEGINRLAYTKTEREARDFLIKQFK
                     LAGLKVRTDYAGNVIARREGRFPDLPVVATGSHIDSVYAAGEFDGTAGVLVALEVMRS
                     LADEGVETDHPLEAIIFACEESARFGASTLGSKAMSGRLDPAYTRSLTDKHGITLMQA
                     FEENGLDLEEVHLAMRFQDEIKAFVELHVEQGPVLERQGKAIGVVSAIAAPIRFHVHV
                     GGTADHSGTTPMDYRHDALLGGAEIALAVEKAALEEVEHGTVGTVGVFSIQPGAMNVV
                     PGAADLYVDIRGTDRDSRQRVVAALESAVNDVAKARGLDIWMDELSSEEPVQMDSQLV
                     AELKAICEEQGVSWLEMPSGAGHDSMNMASLCPTGMIFVPSKDGISHNPAEYTSMEQL
                     MAGAEILRAFMLKQSKASPE"
BASE COUNT          867 a          982 c          872 g         1126 t
ORIGIN      
        1 tgttcggtta attggcctgc ctcttcgtgc cctacatagc ctggagggga gccgatgatt
       61 ttagagacgg catgtttctc catgtattcg ctcatgtcga gtcgaatcag cgtgtcacgg
      121 gaaccgaaga gttcttccgc cagcactttt gtaatttccg ttttaccgac gcctgttgga
      181 ccgacaaata ggaacgatcc gattggacgg gtttttgatt tcaaccctgc gcggcttcgg
      241 cggattgctt ttgcaacttt gtcagctgct tcttcttgcc cgattacttt tttacgcaga
      301 ctttctgcaa ggtctttcat ttttgcttgt tcggaagctt gcagtttcgt cactggaatg
      361 cctgtttttt cttctacgat ttcctcgata tcttcgaccg tcacttcata gagggatgcc
      421 tcgccttgtt cagcttcctc cagttgttga cgtaattgca tttcttgctg gcgcagcgtt
      481 gccgcctttt catagtcttc catttccgca gcttgttctt tctgctggac gacctcttca
      541 agccggtttc cgattgactc cgcatctgca gaagaatgct tcagattcat gcgtgagccg
      601 acttcatcca tcaaatcgat tgccttgtcc ggcaaaaatc ggtcttggat atagcgttgg
      661 gatagtgtga cgaatgcacg aaccgcttca tccgaataac gtacttcatg gaacgcttcg
      721 tagcggtctt taatgccgtt caggatggtg attgcgtctt ccgcagaagg ctctttcacg
      781 atgatcggct ggaagcggcg ctcaagcgct gcgtcttttt cgatttgacg gtactctttc
      841 agcgtcgttg ccccgatgag ctgcatgctg ccgcgcgcca atgccggttt caaaatattt
      901 ccggcatcca ttttggagcc ttccgcagag cctgcaccca cgagaagatg gatttcatcg
      961 atgaatacga tgacatcttt ccggctttcg atttcctcga tcaattgctt catccgctct
     1021 tcgaactgac cgcggatacc ggtgtttgtc acaagtgaag ctacatccaa tacgtagact
     1081 tgtttgttca acagttttgc agggacatcg ccttcatgga tcttcactgc gagcccttca
     1141 gcaatcgctg ttttaccgac acccggctcc ccgataagta ccggattatt tttgttccgt
     1201 ctattcagcg tttcaatgac gcgtttcact tcttgatcac ggccgatgac cgggtcgatt
     1261 tgaccttcct tggcgtcatt cgagatattt ttaccaagtt ggtccaacaa accatttcct
     1321 tgatgttcat cgacttcccg cacttgcgta tgtgcttgcg gattgccgtt cgcttggaaa
     1381 aattggttgg cattcatgcc gaagttcggc agcttcattt gtccttgtac ttcttggaaa
     1441 catgtatgac ataaattgat ctgcattcgt tgttgattca cttggagtcg aaagctcatc
     1501 gtcgcttcgt ttttaccaca atgttgacat ttcataagcc atttcctcct tatatcgttc
     1561 attttattta tagggtctga cattgactat ctttgactta tatatttatt atacattgac
     1621 ctaatttgac tttcaagttt tttgtttcat tcacagtagc aatcattcca cttccccata
     1681 ggatagataa tacatatagg cttttgaaaa ggggcttctc atgaataatt cacttgtttc
     1741 agctggggcg ggacatccac gtattgttgc gtttgctatg ggagatgtaa cgggtgatcg
     1801 agtgattgat caaatctatc tgactggcac gaaaacgccg gatagtccat tcatccaaac
     1861 tataacgctt catgtcaggg acggtagaac aggaataatg acaagtgtcg cgcttcataa
     1921 caatgtaggc tataatccaa ggctgttctt gggggatttc acaggggatg gcatcgacga
     1981 tatcttaatc gccatcgata caggcggcag tggtggaatt gttaactatt acattttttc
     2041 ctacgtcgga aatcaagcaa gactactttt tgattctgac gtttatgctc atcatttcca
     2101 gtatgacgtc acgtaccaag ataattataa agttgaagtt gtcagcagac cgaataacga
     2161 gaggtatatc attgatatct ctaccagaga tgcagattac ttaaatgaaa tatatgacgc
     2221 gaatggaaag ctgaaaagcc cgatagcagg ctttgtggat cctttaagcg gcctgtatcc
     2281 agtcgatttt gatgctaatg gggtctatga gcttttaggt tatcaaaaaa tctccggaag
     2341 atatcacgcg gattccttag gttatataat gaatacactc gaatggaaaa ataatggctt
     2401 tttgttgcgg gagcagtatt tggctatatt cggttcacag ttgcagcaat gaaagagtac
     2461 gataaaaaaa caggtggaga aaaacaatcc gttttcctcc acctgtttta ttctggcgat
     2521 gctttcgact gtttcaacat gaaagcgcgt aatatttccg cacctgccat aagctgctcc
     2581 attgacgtat attcggccgg attgtggctg attccgtcct tcgacgggac gaaaatcatg
     2641 ccggtcggac agagcgatgc catgttcatc gaatcatggc ccgcaccgct tggcatttcg
     2701 agccacgata ctccctgttc ttcacagatt gcttttaatt cggccacgag ttggctgtcc
     2761 atctggacag gctcttcgct cgatagttca tccatccaaa tgtccaggcc gcgtgctttc
     2821 gccacgtcat tgaccgctga ttcgagtgca gcaaccaccc gctggcgtga atcccggtcc
     2881 gtcccccgga tgtccacgta caggtcagcc gcgcccggta ctacattcat cgcgcctggc
     2941 tgtatggaga agaccccgac ggtgccgacc gtgccatgct caacctcttc gagtgctgcc
     3001 ttctcgacag caagggcgat ttccgcgccg ccaagcaacg catcatgacg gtaatccatc
     3061 ggcgtcgtgc cggaatgatc cgctgtccct ccgacatgca catgaaaacg gatcggcgcc
     3121 gcaatggcgg agacgacgcc aatcgccttc ccctgtcttt ccagcacggg tccctgttcc
     3181 acatgcagct cgacaaacgc tttgatttca tcctgaaagc gcattgccaa atggacctct
     3241 tccagatcga gaccattttc ttcaaatgcc tgcatgagcg ttattccgtg tttgtcagtg
     3301 agtgaccggg tgtacgccgg atcgagacgg cctgacatcg ccttgctgcc aagggtcgat
     3361 gcgccgaagc gggcggattc ctcacaagca aaaatgatgg cttcaagcgg atgatccgtc
     3421 tccacgcctt catctgcgag ggaacgcatc acttcaagcg cgacaaggac acccgcggtt
     3481 ccgtcaaatt cacccgctgc atagacggag tcgatatgcg agcctgtcgc gacaaccggc
     3541 aggtcgggga atctcccctc cctgcgtgca atgacgtttc ccgcatagtc agttcgcact
     3601 ttcagcccgg caagtttaaa ttgcttaatc aaaaaatctc gggcttcccg ctccgtcttc
     3661 gtgtaggcca gccgattgat gccctctccc ttttccgtga atcgtgcaag ttcttcaaat
     3721 gactgacgca accgttccag cttcatggcc cttcccccaa ttcttatact tcatttcaaa
     3781 aaatcttttt ttatgttttc aagtccaatg atattcaatt ataccaatat acagacagta
     3841 atggtgc
//
LOCUS       DASULI010000317         5031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_767401, whole
            genome shotgun sequence.
ACCESSION   DASULI010000317 DASULI010000000
VERSION     DASULI010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767401"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFX34_06765"
     CDS             complement(<1..299)
                     /locus_tag="VFX34_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX5564644.1"
                     /translation="MLLKTRKNNIEIDGLVALEKRLERGDVNRSKIEERLYNLQAGFA
                     GETQYDKYLSEFKPRYPHAILHDVTLCEEDVFFQMDSILITPAFIVISEVKNIAE"
     gene            complement(752..1120)
                     /locus_tag="VFX34_06770"
     CDS             complement(752..1120)
                     /locus_tag="VFX34_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564645.1"
                     /translation="MIFVQGPPVYVKVAVLEEESTSVFVPNEGSAREAIDIQPSVETA
                     SHVVNPAIMNKLRYLSEPFRKQVYTPLQFICEDGIVKGAIQKVEGDILWIEADDQITE
                     VEIGKVEDVLWRGQPFNIRS"
     gene            complement(1232..2605)
                     /gene="mgtE"
                     /locus_tag="VFX34_06775"
     CDS             complement(1232..2605)
                     /gene="mgtE"
                     /locus_tag="VFX34_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5564646.1"
                     /translation="MTVNEEIRDEIIIDEEKLINSLEQQDIKTFRNEYLMLHPYDRAV
                     FYEKVDTDHRKTMYFFLSPKELAEIFETSEIDEDEYKQFLQEMDTTYAADMISHMFVD
                     NAVDVLKELDKAQIASYLTLMNKEAAAQIKSLLHYEEYTAGSIMTTEYVSIPQNSTVR
                     SAMTILRNEAPSAETIYYVFVVDEDNRLTGVVSLRDLIITDEDTLIQTIMNERVVSVL
                     VSDDQEDVARMTQDYNLLAVPVVDFQQHMLGIITVDDIIDVLDEEASDDYSKLAAVSN
                     MDSFDKTSFAAAKKRIPWLIILLVLGMLTANLIDLFTDTISQVALLAAFIPLIAGTAG
                     NSGTQALAVAVRGIATRDVEDESKFKLLLREAGTGLITGLICAIFVVGLIFIWKHEFI
                     IALLVGAAILVSIFVATISGSFIPLFMHRMKIDPAVASGPFITTLNDVISIIIYLGLA
                     TMFISSL"
     gene            2694..3434
                     /gene="prpE"
                     /locus_tag="VFX34_06780"
     CDS             2694..3434
                     /gene="prpE"
                     /locus_tag="VFX34_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase PrpE"
                     /protein_id="HEX5564647.1"
                     /translation="MKLDIIGDIHGCYDELLSLVEKLGYSFASGLPFHEDGRQLAFVG
                     DAMDRGPASLKTLELMFKLQDNHRLIYSPGNHCNKLYRLAKGSNVQKKNGLETTVAEL
                     NALPPKEKTRFLDRYRQFYEALPLYENLDNGKLIIAHAGIREQMIGEPFSKKIQSFVL
                     YGDTTGETLPDGRPVRRDWAKKYFGEAWIIYGHTPVTEARFVNRTVNIDTGCVFGGKL
                     TAVRYPEMEIVAVPSQQPHIPEKFTHFD"
     gene            complement(3422..4339)
                     /locus_tag="VFX34_06785"
     CDS             complement(3422..4339)
                     /locus_tag="VFX34_06785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5564648.1"
                     /translation="MNEKKFTLKYTVEEDTVLLREFLHGHGISKRTLTAVKYDGGCLL
                     VNGTEQTVRHMLRRGDTVIVQFPPEEPSAGLVPESGNLSIVYEDQAILIIDKPAGQGT
                     IPSRDQPNGTVANFVAGKFEADHVPSTVHVVTRLDTDTSGLVCIAKNRHIHHLLSEQM
                     QMTGFMRRYRAIVEGYIEEDAFTINQPIGRKDGSIIERVVRQDGQYARTDVQVHKHFE
                     RNGMPYTSVSLKLHTGRTHQIRVHMQWLGHPLAGDDLYGGSLILIGRQALHCAAIGFL
                     HPLTKEQMTFTSGLPVDMERLMAVPNQSK"
     gene            complement(4347..>5031)
                     /locus_tag="VFX34_06790"
     CDS             complement(4347..>5031)
                     /locus_tag="VFX34_06790"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX5564649.1"
                     /translation="LSVGGDGTLLHAFHKYIHRLKDTAFIGIHTGHLGFYADWKPAEL
                     EKLVLSIARQEYNVIEYPLLEVTINYRHSEKAAKYLALNESTVKSPEVTLVMDVELNG
                     VHFERFRGDGLCMSTPSGSTAYNKALGGAIIHPSLHAIQLTEMASINNRVFRTVGSPL
                     ILPAHHSCVLTPVKGPDFMVTIDHLQLLHKDVKSIEYKVADEKVRFGRFRPFPFWTRV
                     HDSFIASDE"
BASE COUNT         1286 a         1228 c         1155 g         1362 t
ORIGIN      
        1 ttttctgcga tgttttttac ttctgaaatt acgatgaaag cgggcgtgat caatattgaa
       61 tccatctgaa aaaagacgtc ctcttcacat aatgtgacgt catgcaaaat agcatggggg
      121 taacgcggtt taaattctga taagtatttg tcgtattgtg tttcccctgc aaatcctgct
      181 tgtaaattgt atagccgttc ttctattttt gacctattta catctcccct ttccaaccgt
      241 ttttccaatg caaccaatcc gtcaatttca atattatttt tccgtgtttt tagtagcact
      301 tcatcacctc accttcattg tagcttggct tgtcggaatt tggaatagtc tgactcaaat
      361 tgtcagaagt gatttctgga ttaagtcatt aaccgcggag gtatccgttt ttgccaatta
      421 aaaaatacgc gccgaatgct tttgacaaga tgaaatagtt gagaatctgt tgcgaattag
      481 aatataggag ctcttatgtg attaattgag cacttatgca aataatggag cctttgtccg
      541 attaattgag cccttgcaca agtaattgag cctttgtgtg attaattgag cccttgatca
      601 aataatggag tccttgtgca attaatagag cccttgatca aataatggag cccttgtgcg
      661 attaattgag cccttgatga aataatagag cccttgtgca actccaacct aaaaaatcaa
      721 gtcggaccgt aaattccggt ccgacttgtt tttatgatcg aatgttaaat ggctgcccgc
      781 gccataaaac gtcttctacc tttccgattt caacttcggt gatctggtca tcggcttcaa
      841 tccagaggat gtcgccttcc actttttgaa tcgcgccttt cacaataccg tcttcacaga
      901 tgaattgaag aggcgtgtat acctgcttcc ggaacggttc gcttaaatag cgcagtttat
      961 tcatgatggc ggggttgaca acgtggcttg ctgtttcaac ggatggctgg atgtcaatcg
     1021 cttccctcgc cgagccttcg ttcgggacga agacggacgt gctttcttcc tcaaggacgg
     1081 ccacctttac atagacggga ggcccctgca caaatatcaa cggttcattg taggaaatgt
     1141 tgattccccc ttttttccgt agtatatgcg cccatgggga ttgacgttct tttcctcatt
     1201 ctatgttcac tataccaatc aaagatgacc ttcaaaggct gctgatgaac attgtcgcca
     1261 ggccaaggta gatgatgatg ctgatgacgt cgttcaacgt cgtgatgaac gggccggatg
     1321 cgacagcagg atcgattttc atccggtgca tgaacagcgg aatgaacgaa ccggaaattg
     1381 tcgcgacaaa gatggaaact aaaatggctg ctccgacgag caaggcgatg atgaattcat
     1441 gtttccaaat gaaaatgagg cctacgacaa agatggcgca aatgagaccg gttatgaggc
     1501 cggtgcctgc ttcgcgcaac agcaatttga atttgctctc atcctcgacg tcacgagtcg
     1561 cgataccgcg gacggcgact gccagcgctt gcgtcccgct atttcctgca gtccctgcaa
     1621 tcaacggaat gaatgcagcc agcaaagcga cttgagaaat cgtatcggtg aatagatcga
     1681 tcagatttgc cgtcaacatg ccaagcacca ataggatgat cagccacgga atccttttct
     1741 tcgccgctgc aaatgacgtc ttgtcgaaag agtccatatt ggatacggca gcaagtttgg
     1801 aatagtcatc ggatgcttct tcatctaaaa cgtcaataat atcatcgaca gttatgattc
     1861 ccaacatatg ctgctggaaa tcgacgaccg ggaccgcgag caagttatag tcttgtgtca
     1921 tccttgcaac gtcttcctgg tcatccgaaa caaggacgct gacgacacgc tcattcatga
     1981 ttgtctggat taacgtgtcc tcgtccgtaa tgatcaagtc ccgaagcgaa acgacgcccg
     2041 tcaaacgatt gtcttcatcc acgacgaaaa cataatagat cgtctccgcg gacggggctt
     2101 cattccgcag aatcgtcatc gctgagcgca cagtcgaatt ttgcggaatc gaaacgtatt
     2161 ccgtcgtcat gatcgaaccg gcggtatatt cttcataatg caagagggat ttgatttgtg
     2221 cagccgcttc tttgttcatt aacgtcaaat agctggcaat ctgcgcctta tcaagttctt
     2281 tcaggacatc taccgcattg tcgacgaaca tgtgggagat catatccgcg gcataagtcg
     2341 tgtccatctc ctgaaggaac tgcttgtatt catcttcatc gatttcactt gtctcgaata
     2401 tctccgccaa ctctttcgga gataggaaga aatacatcgt cttccgatga tccgtgtcca
     2461 ccttctcata gaaaacagct cggtcatatg ggtgaagcat tagatattca tttcggaacg
     2521 ttttaatatc ttgctgttcc aatgaattta tcagtttttc ttcgtcgata atgatctcat
     2581 cgcgaatttc ttcatttaca gtcatggctg caccttcctt tccgctccga catgcatgaa
     2641 tgcattgcta aaggtatgat aataaataag acaatcacta aaggagaatc aatatgaaac
     2701 tggatataat cggtgacatt cacggttgct acgatgaatt gctgtcgctc gtcgaaaagc
     2761 ttggctattc gtttgcatcg ggattaccgt tccatgagga tggacggcag ctcgcttttg
     2821 taggcgacgc gatggaccgg ggtccagcat cgttgaaaac gctggaattg atgttcaagc
     2881 tacaagataa tcatagactt atctattccc ccggcaacca ttgcaacaaa ctttaccggc
     2941 ttgcaaaagg cagcaacgtc caaaagaaaa acgggttgga gacgacagtg gcggaattga
     3001 atgccctgcc tcccaaagag aaaacccgtt ttttggatcg ctatcggcaa ttctatgaag
     3061 cattgccgtt atacgaaaat ctggacaacg gaaaactcat tatcgcacac gcgggaatcc
     3121 gtgaacagat gattggagaa ccattttcaa aaaaaataca gtcgttcgtc ctatacggcg
     3181 atacgaccgg tgaaaccctc ccggatggcc gtcctgtacg cagggattgg gcgaaaaaat
     3241 acttcgggga ggcatggatc atttacggac atacacctgt taccgaggca cgattcgtca
     3301 accggaccgt caatatcgat acgggctgcg tgttcggggg caagcttacc gccgtccgct
     3361 atccggaaat ggaaatcgtc gccgttccat ctcagcaacc gcatattccg gagaaattca
     3421 ctcattttga ctgattcgga acggccatca atcgttccat atcaacaggc aatccgctcg
     3481 taaatgtcat ctgttctttc gtcaatggat gaaggaagcc gattgcagca caatgcaatg
     3541 cttgcctgcc gatcaatatg agcgaaccgc catacaagtc atcccccgca agcgggtggc
     3601 caagccattg catatggaca cggatttgat gggtccgccc tgtatgcaac ttcaatgaaa
     3661 cggatgtata cggcatgcca tttcgttcaa aatgtttgtg cacttggaca tcggtccttg
     3721 catattgtcc atcttgccgt accacccgct cgatgatgct gccatctttc cggccgatcg
     3781 gctgatttat cgtgaacgca tcctcttcta tatacccttc cacgattgca cgataacggc
     3841 gcataaaacc ggtcatctgc atctgctcac tgagcaaatg gtgaatatga cggtttttcg
     3901 ctatgcaaac aaggcccgac gtatcggtat ccagccttgt gacgacatgc acggtcgatg
     3961 ggacgtgatc ggcttcgaat tttccggcca caaaattggc aaccgtccca ttcggctgat
     4021 cgcgtgatgg gatcgtccct tgaccggccg gcttatcaat gatcaaaatc gcttgatcct
     4081 catagacaat tgacagattg ccggattcag gcacaagacc ggcgcttggc tcttccggag
     4141 ggaattggac aattacggta tcccctcttc tcagcatatg cctcaccgtc tgctccgtcc
     4201 cgttcacgag cagacatccg ccatcatatt tcaccgccgt caatgtccgt ttggaaattc
     4261 catgtccgtg caggaattcc cgtagaagga ccgtatcctc ttcgacggta tatttgaggg
     4321 tgaacttctt ttcattcatg accgtttcac tcatcacttg caatgaacga atcgtggacc
     4381 ctcgtccaga atgggaacgg acggaatctg ccgaaacgca ccttctcatc cgccactttg
     4441 tattcgatcg attttacatc tttatgcaac agctgcaaat gatcgattgt caccatgaag
     4501 tctggccctt tgactggcgt cagcacacaa ctatggtggg ctggcagtat aagcggagag
     4561 cctaccgtcc ggaaaacacg gttgttgatc gaagccattt cggtcagctg gatggcgtgc
     4621 aaggatgggt ggataatcgc gccaccaagc gctttattat aggcggtcga gcccgatggc
     4681 gttgacatgc atagtccgtc cccgcggaat ctttcaaaat gaacaccgtt caattccacg
     4741 tccatgacga gcgtcacttc aggggatttt accgttgatt catttagcgc aaggtacttt
     4801 gccgcctttt cggaatggcg gtaattgatc gtcacctcga gaagagggta ttcgatgaca
     4861 ttgtattcct ggcgggcaat cgacaggaca agcttttcga gctccgctgg cttccaatcg
     4921 gcatagaatc cgagatgccc agtatggatg ccgatgaatg ccgtatcttt caggcgatgg
     4981 atgtatttat ggaaggcgtg cagcaatgtg ccgtcccctc cgaccgataa a
//
LOCUS       DASULI010000318         7320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_729214, whole
            genome shotgun sequence.
ACCESSION   DASULI010000318 DASULI010000000
VERSION     DASULI010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729214"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2299)
                     /locus_tag="VFX34_06795"
     CDS             complement(<1..2299)
                     /locus_tag="VFX34_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5564650.1"
                     /translation="MDALIDLFLQDKEITKVVEELKTGQDRQLLAGLSGGAKAVFFKT
                     VQQSAQKPILIITPNMLQAQRTYEDLVKMLGDSLVHLYPAEELIAADFSVSSYELRAN
                     RIDTLDHMARVGKGIYITPVAGMKKLLPPKERWLDNSLSVNLDDSIDVDEWTRKLVEM
                     GYGRQDMVTAPGDFALRGGILDVYPLNMENPIRIELFDTDVDSVRAFSAEDQRSTDKL
                     KEVTILPAMEHVWGAAELSQIASNLEDALSASLKRMKNEEAKESLTLNITRDIGLLRD
                     GITPEGMMKYVSFSDRAPSYLGDYFPADGIVLFDEIGRILEVSSSLEKEEKEWFLTLL
                     EEGKIVHDAKISFSFEEMHKMLDQPKSYLSLFVRAVPGITLKKNVSFSCKPMQEFHGQ
                     MNLLKNEMERWQQGKYNIFIVADGEERMKKVQSILRDYEMDAALNGKSKDAGTISIIE
                     GDLSAGFELPFQRLAVITDAELFKGKPKRKARPQKMTNAERIKSYSEIKPGDYIVHVH
                     HGIGKYYGVVTLEVGGVNKDYLDIRYRGEDKLFVPADQIDLIQKYVASGEKEPKLHKL
                     GGAEWKKTKSKVSSAVKDIADDLIKLYAQREAEKGYAFSADDDLQRSFENAFPYDETE
                     DQLRSIAEVKMDMERERPMDRLLCGDVGYGKTEVAIRAAFKAVADGKQVAFLVPTTIL
                     AQQHFETMKERFSGFPVEVSLLNRFRTRKEQTETLKGLKAGTIDVVVGTHRLLSKDVV
                     YKDLGLLIVDEEQRFGVTHKEKLKQL"
     gene            complement(2379..2627)
                     /locus_tag="VFX34_06800"
     CDS             complement(2379..2627)
                     /locus_tag="VFX34_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-F factor Fin"
                     /protein_id="HEX5564651.1"
                     /translation="MKPMKPKDIRYSCRHCNTEVGSIPLSSAEEIIRELQKVEEGQDE
                     HFLEYDENGSMTVRCICEQCEQSMRMNPDYYALKKWLQ"
     gene            complement(2675..3238)
                     /gene="pth"
                     /locus_tag="VFX34_06805"
     CDS             complement(2675..3238)
                     /gene="pth"
                     /locus_tag="VFX34_06805"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5564652.1"
                     /translation="MKMIIGLGNPGKQYEKTRHNVGFHAIDELARRLDAPAMQLKFNG
                     AYTIIHRPEGKVMLVKPLTYMNLSGECVGPLMDYYDVELEDIVVLYDDLDLAPGKLRL
                     RQKGSAGGHNGMKSLIAHLGSESFNRIRIGIGRPTGGMKVADYVLSPFSKEEQPLIEE
                     MVGKSASACVDWLKSPFLDVMNNYNGA"
     gene            complement(3311..3946)
                     /locus_tag="VFX34_06810"
     CDS             complement(3311..3946)
                     /locus_tag="VFX34_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX5564653.1"
                     /translation="MSTAIQSKTRETKTRSALSALRNDGFIPSVVYGYKTESTPIAVK
                     EGDLLKTLREVGRNGVIKLEVDGKELNVVLSDFQEDALKGEIRHADFLAINMTEEYDV
                     NVTVNLIGESLGEKEGGVLQQPNREVTIKVKPSDIPESFDVDISELQIGDTITIADVR
                     KKSKFEILNDDDYALVLISAPRSEEELEELEADTGASDAEPEVIGEKEEED"
     gene            complement(4029..4988)
                     /locus_tag="VFX34_06815"
     CDS             complement(4029..4988)
                     /locus_tag="VFX34_06815"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007084185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX5564654.1"
                     /translation="MANHYPNDKLKIFSLNSNETLAQEVADEIGRPLGKCSVKRFSDG
                     EIQINIEESIRGCDVFVIQSTSYPVNDNLMELLIMIDALMRASARTINVVMPYYGYAR
                     QDRKARSREPITAKLVANLLEKAGADRVIAIDLHAPQIQGFFDIPIDHLVAEPILTEY
                     FKGKGLEGNELVIVSPDHGGVTRARKMADRMKAPIAIIDKRRPRPNVAEVMSIVGNVE
                     GKTAILIDDIIDTAGTITIAANAIIENGAKEVYACCTHPVLSGPAIERIDNSQIKELV
                     ITNSIELPESKKSPKIKQLSIAKLLGEAVIRVFEEKSVSTLFE"
     gene            complement(5003..6373)
                     /gene="glmU"
                     /locus_tag="VFX34_06820"
     CDS             complement(5003..6373)
                     /gene="glmU"
                     /locus_tag="VFX34_06820"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5564655.1"
                     /translation="MTNTYAIVLAAGQGTRMKSDLYKVLHPVCGKPMVEHVVDHILGI
                     GADKIVTIVGHGAEMVEETLGAKSEYALQAEQLGTAHAVQQAEDRLGELEGITLVVCG
                     DTPLIKSETMKALIAHHSDTKAKATILTAHADDPTGYGRIIRDEQGNVLRNVEHKDAS
                     PEERKVTEINTGTYCFDNRALFEALKKVRNDNAQGEYYLPDVIGILKAEQALISAYVT
                     DDFSETIGINDRVILSEAEKVMRQRIAESHMRNGVTIIDPSSTYISADAKIGRDAVLQ
                     PGTMIEGQTVIGEKCIIGPNSHIANSEIGESTTIHSSVVRDSTIGSGTTVGPFAHIRP
                     ESKLSDNVKIGNFVEVKKTKLGEGSKVSHLSYIGDAEVGTHVNIGCGTITVNYDGKNK
                     HTTTIEDNSFIGCNSNLVAPVKIGKGAYVAAGSTITKDVPESSLAIGRARQENKEGYA
                     TKLNLN"
     gene            complement(6741..7025)
                     /gene="spoVG"
                     /locus_tag="VFX34_06825"
     CDS             complement(6741..7025)
                     /gene="spoVG"
                     /locus_tag="VFX34_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX5564656.1"
                     /translation="MEVTDVRLRKVETDGRMRAIASITLDGEFVVHDIRVIEGNDGLF
                     VAMPSKRTPEGEFRDVAHPINTSARMKVQDAVLSAYHMVEEKGILEEAGA"
     gene            complement(7200..>7320)
                     /locus_tag="VFX34_06830"
     CDS             complement(7200..>7320)
                     /locus_tag="VFX34_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX5564657.1"
                     /translation="NDVYAQHFGSHTPARSTVEVARLPKDATVEIEVIALAGE"
BASE COUNT         1629 a         1877 c         1615 g         2199 t
ORIGIN      
        1 tcagctgttt cagcttttcc ttatgtgtca cgccgaaccg ctgctcttcg tcgacgatga
       61 gcagtcctag gtctttatag acgacgtctt tcgaaaggag acgatgcgtg ccgacgacga
      121 catcgattgt gccggccttc aaccctttta acgtctccgt ctgctccttc cgcgtgcgga
      181 aacggttcaa tagcgacact tccaccggaa aaccagagaa ccgctccttc atcgtctcga
      241 aatgctgctg ggcaagaatc gtcgtcggta caaggaaagc gacttgtttg ccgtccgcga
      301 ccgctttgaa cgcagcccgg attgccactt ccgtcttccc gtatccaaca tcgccgcaaa
      361 gaagccgatc catcggacgc tccctctcca tatccatctt cacttctgcg atggaacgta
      421 gctggtcttc cgtctcgtca tatggaaacg cgttttcaaa ggaacgctgc aagtcgtcgt
      481 ctgcagagaa cgcataccct ttttcagctt cacgctgtgc atagagctta atcagatcat
      541 ccgcgatatc cttgactgcg gatgatactt tgctcttcgt cttcttccat tccgcaccgc
      601 cgagtttatg gagtttcggt tccttctccc cggatgcgac gtatttttga atcagatcga
      661 tttgatccgc tgggacgaaa agtttgtcct ccccacgata gcggatatca agatagtctt
      721 tattgacgcc gcccacttcg agcgtcacga cgccataata tttaccgatg ccgtgatgga
      781 cgtggacgat atagtcgcca ggcttgattt ccgaatagct tttgatgcgc tctgcattgg
      841 tcattttctg cggtcgggct ttccgcttcg gtttcccttt gaacagctct gcatccgtaa
      901 tgaccgcaag acgttggaaa ggtagttcaa accccgcaga taaatcccct tcgatgatgg
      961 agattgttcc tgcatctttc gacttgccat tcaatgctgc atccatctcg taatctctta
     1021 gaatcgattg gactttcttc attcgctctt cgccatcggc aacgatgaag atattgtatt
     1081 tcccttgctg ccagcgttcc atttcatttt tcaacaagtt catctgcccg tggaattcct
     1141 gcatcggctt gcaggaaaat gaaacatttt tctttagggt gattcccggc acggccctga
     1201 cgaacaacga taagtatgac tttggctgat caagcatttt gtgcatctct tcgaatgaaa
     1261 atgaaatttt cgcatcatga acaatcttgc cttcctcaag aagcgtcaga aaccattctt
     1321 tttcttcttt ttcgagtgac gaagacactt ccaggattcg tccgatttca tcgaacagaa
     1381 caattccatc cgctggaaaa taatcgccaa gataggatgg ggcacggtct gaaaacgata
     1441 catatttcat catcccttcc ggagtgatgc cgtcccgcaa aaggcctatg tcccgggtga
     1501 tgttcagtgt cagggattct tttgcctctt cattcttcat ccgcttcaaa cttgcactga
     1561 gggcgtcctc caaattcgag gcaatttgcg atagttcagc ggcgccccaa acatgttcca
     1621 tcgcgggaag gatcgtaact tctttcaatt tgtcagtcga gcgctgatct tccgcggaaa
     1681 atgcccggac ggagtcgaca tccgtatcga acagctcaat ccggatcggg ttttccatat
     1741 tcaatggata cacgtccaaa atgccgcccc gaagagcaaa atcgcccggc gcggttacca
     1801 tgtcctgtct accatagccc atttccacaa gctttcgcgt ccattcgtcc acatcgatac
     1861 tgtcatccag gttgactgac aaggaattat cgagccatct ctctttagga ggaagaagtt
     1921 tcttcattcc cgcaacaggc gtaatataaa tccctttgcc gacgcgcgcc atatgatcga
     1981 gcgtatcgat ccggttcgcg cgcagttcat aactggacac agaaaaatcg gcagcgatca
     2041 attcctctgc cggatacaaa tgaacaagtg aatccccaag catcttcaca aggtcttcgt
     2101 atgtacgttg tgcttgcagc atgttgggag tgatgatcag aatcggcttc tgtgcagatt
     2161 gctggacagt cttaaaaaag acggcttttg caccgcccga tagtcccgcc agcaattggc
     2221 ggtcctgtcc tgttttcaac tcctcaacta ctttagtaat ctctttgtct tgtagaaata
     2281 aatctatgag cgcatccaat gattatttgc ctcgctttgc agtaaataaa ttgaagtgaa
     2341 aaaagctttg cgcgcgcaat acgccaaagc agaaatttct attgtagcca tttctttaac
     2401 gcatagtaat ccggattcat tcgcatcgac tgttcacatt gctcgcaaat gcaacgaacc
     2461 gtcatcgaac cgttttcatc atactccaag aaatgctcgt cttgcccttc ttccaccttt
     2521 tgcagttcac ggatgatctc ttcggcagat gagagcggga tgctgccgac ctcggtgttg
     2581 caatgtctgc atgaataacg gatgtccttc ggtttcatcg gtttcatcgg ccccttttta
     2641 acaaagtata ggctagggag ccgacgatta ttccttatgc gccattatag ttgttcatta
     2701 cgtctaaaaa tgggctcttt agccaatcca cgcaggcgga agcgcttttt cccaccatct
     2761 cttcgatgag cggctgttct tctttgctga atggcgatag tacataatct gccactttca
     2821 tgccgcctgt cggacggccg atgccgatgc ggatcctgtt gaaggattcg gagccaagat
     2881 gtgcaatcaa tgatttcatg ccgttatggc cgccggcgct tcccttttgg cgcaatcgca
     2941 atttgccagg ggcgaggtcc aggtcgtcgt agaggacgac gatgtcttcg agctccacat
     3001 cataataatc catgagcgga cctacacatt ctccggaaag gttcatgtat gtcaaaggtt
     3061 tgacgagcat cacttttcct tccgggcggt ggatgatcgt ataggcgccg ttgaatttca
     3121 attgcatggc gggtgcgtcc agtcgacggg ccaattcatc gattgcatgg aagccgacgt
     3181 tatgtcgggt tttctcgtat tgctttcccg ggttgccgag tccgataatc attttcataa
     3241 ggcatatctt ccttttgaat aaattataaa aagggcgcac gctttgcacg tgcgctccaa
     3301 ctgttgttta ttaatcttcc tcttcttttt caccgatgac ttccggttct gcatctgaag
     3361 cgccagtgtc agcctcaagc tcttccaatt cctcttcaga acgtggggca gagataagga
     3421 cgagtgcata atcatcatcg ttcaaaattt caaacttgga tttcttacgc acatctgcga
     3481 tggtgatcgt gtcaccgatc tgcaattcgg aaatgtctac atcgaacgac tccggaatat
     3541 ccgaaggttt gactttgatc gtcacttcgc ggtttggctg ctgtagtacg ccgccttcct
     3601 tctcgcctag ggattcgccg atcaggttga ccgtgacgtt gacatcatat tcttccgtca
     3661 tgttgattgc caggaagtcc gcgtggcgga tttcgccttt tagcgcatct tcttgaaaat
     3721 cgctcaatac gacgttcagt tctttgccgt cgacttcaag cttgatgacg ccatttcgtc
     3781 cgacttcacg caaagttttt agcagatcgc cttcttttac ggcaatcggc gtagattctg
     3841 ttttatatcc ataaacgacc gatgggatga atccatcatt tcttagtgct gataatgctg
     3901 atctcgtttt tgtttctctt gttttcgact gaattgctgt actcatttgc gtagtcacct
     3961 ttcctgaaaa aagttattct atctcttgaa atacccataa tcagacgaca tgaaactaac
     4021 tagattgctt attcaaacaa cgtgctgaca gatttttctt caaatacacg gataactgct
     4081 tcacccaaaa gtttagcaat cgataattgc ttgattttcg gtgatttttt gctttccggc
     4141 aattcgattg aattcgtaat gaccaattcc ttgatttgtg aattgtcgat tctttcgata
     4201 gcagggccgg atagcaccgg atgtgtacag caagcgtaca cttcttttgc gccgttctcg
     4261 atgatcgcgt tcgctgcgat tgtaatcgtc ccggcagtat cgatgatgtc atcgatcaga
     4321 atagctgttt tcccttcgac attccctacg atgctcatca cttcggcaac gtttggacgt
     4381 ggacggcgtt tgtcgatgat cgcgattggg gctttcatgc gatccgccat tttccgggca
     4441 cgtgtgacgc cgccatggtc cggagatacg atgacgagtt cgttgccctc caagcctttt
     4501 cctttgaagt attccgtcaa aatcggctct gctacgagat ggtcgatcgg aatgtcaaag
     4561 aagccctgga tttgcggtgc gtgcagatca atggcgatga cgcggtctgc acccgctttt
     4621 tcaagcaggt tcgctaccag cttcgctgtg atcggttcac gggagcgcgc tttgcggtct
     4681 tggcgtgcat agccgtaata cggcatgaca acattgattg tacgtgctga agcgcgcatc
     4741 agcgcgtcaa tcatgataag gagctccatc aggttatcat tgactggata agaggttgat
     4801 tggatgacaa atacatcgca gcctcggata ctttcttcga tattgatttg aatttctccg
     4861 tcgctgaaac gtttgacgga gcacttgccg agtgggcgtc cgatctcgtc agccacttct
     4921 tgcgcaagtg tttcgtttga atttaaagag aatatcttca gtttatcatt aggataatga
     4981 ttagccatca tggtccccct ggttagttaa gatttaattt tgttgcgtat ccttctttat
     5041 tctcctggcg cgcgcgccca attgcaagtg aactttctgg cacatctttt gtaattgtgg
     5101 atccggccgc tacataagcg ccttttccaa tttttacagg tgcgactaga ttggaattgc
     5161 aaccgataaa tgagttatct tcaatcgttg tcgtatgctt gtttttaccg tcgtaattca
     5221 ccgtgatcgt tccgcagccg atgttgacat gtgtgccgac ttccgcatca ccgatatagc
     5281 tgaggtggga aaccttgctt ccttctccta gctttgtctt tttcacttcg acaaaattgc
     5341 cgatttttac attgtcgctg agtttggatt ccggacggat atgtgcgaac gggcctactg
     5401 tagtaccgga cccgatggtg ctgtcccgta caactgacga atggatggtt gtagactcgc
     5461 caatttcact gttggcgata tgactgtttg gtcctatgat acacttttcc ccgatgaccg
     5521 tctgtccttc gatcatcgtg cctggttgaa gaactgcatc ccggccgatt tttgcatcgg
     5581 cactaatata cgtgctgctt gggtcaatga tggtaacgcc attgcgcata tgggattctg
     5641 caatcctttg gcgcatcacc ttttccgctt ccgataggat gaccctgtcg ttgataccga
     5701 tcgtctcact gaaatcatcg gttacatagg cggagatcaa tgcttgttca gccttcagga
     5761 taccgatgac gtctggcaag tagtattcgc cttgcgcgtt atcgttcctc acttttttca
     5821 aagcctcgaa cagcgcgcga ttgtcaaagc agtacgttcc cgtattgatt tcggtcactt
     5881 tccgctcttc cggtgatgca tccttatgtt cgacattgcg aaggacgttt ccttgctcat
     5941 cccggatgat acggccgtag cctgtaggat catcggcgtg cgcggtcagg attgtcgctt
     6001 tcgccttcgt gtcggagtgg tgcgcgatca atgctttcat cgtttccgat ttgatgagcg
     6061 gtgtgtctcc gcagacgacg agcgtaatcc cttcgagttc gcctagccgg tcctccgctt
     6121 gctgaaccgc atgtgctgtg ccaagctgtt ctgcctgaag ggcatattcg cttttggcac
     6181 ccagtgtttc ttctaccatc tctgcgccgt ggccgacaat cgttacgatt ttatccgcgc
     6241 cgattccgag gatatggtcg acaacatgtt cgaccatcgg ttttccacag acaggatgca
     6301 atactttata taaatcggac ttcatccgcg tcccttgccc tgcagctagg acgatggcat
     6361 atgtattcgt catttgctgg cctccatcca taatttcatt attgactata gccgaaaata
     6421 atataaattt caactatccc ctcttgacaa cgggttgtaa ttcttttaat attgttcttt
     6481 tcatttttaa caggcaatga ataacaaaaa aaagctgtca cagaaagtca tgtttactga
     6541 cttctgcaca gccaatcgaa cagcgaaggg ttcgatttgc cgtatttctg cgttctttgc
     6601 agaaattaag gcatccctta attatcctcg caaaagccgt tcttcgttac agcttttgat
     6661 gcaatccttc tttgaaataa aagaagccgt tacagaatga cttctgcaca gcctctgtga
     6721 tgcttttcaa ctagtataac ttatgcaccg gcttcttcca atatcccttt ttcttcgacc
     6781 atatggtacg cacttaggac cgcatcttgg actttcatcc ttgcagacgt attgatcgga
     6841 tgtgccacgt cgcggaattc cccttccggc gttcgtttgc tcggcatcgc gacaaacaat
     6901 ccgtcattcc cctcgatgac acggatgtca tgtacgacga attcaccatc caaagtaatt
     6961 gatgcgattg cccgcattcg gccgtctgtc tcaactttcc ttagtctcac atctgtcact
     7021 tccattattt ccccactccc tttgttgttc gtctattatt cgtctcaacg attatattct
     7081 acaaaagtaa gcatttccct tttatttttc caaacttttt ctaaaaactt tttcagcgac
     7141 tttttttggc aaataaaaat gacggcctgc tcaattccga gcgtgccgcc acttgtcatt
     7201 tattcccctg caagggcgat gacttcgatt tcaactgttg catccttcgg caatcttgcc
     7261 acttccactg tggaacgcgc gggagtatgt gagccgaaat gctgtgcata tacgtcgttc
//
LOCUS       DASULI010000319         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1663133,
            whole genome shotgun sequence.
ACCESSION   DASULI010000319 DASULI010000000
VERSION     DASULI010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663133"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1683)
                     /locus_tag="VFX34_06835"
     CDS             complement(115..1683)
                     /locus_tag="VFX34_06835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5564658.1"
                     /translation="MKTIWHNGKLYTMEKEGETVEALLTENGKIIAMGTYEELKSEAE
                     NEVDLDGAVLYPGFIDNHMHMIGHGQKLLSLDLSKAKSSDEMMDMLMNAYPDLKEDEW
                     FIGEGWNENNFADKKIFTRYELDKVTNSPMYLKRTCRHAAIVNSKALELAGITKDTPD
                     PDDGVIERDENGEPTGVLKEGPMDRVLQLIPEPTEEMLTKALEKSVDDLFSKGLTGAV
                     TDDLGYYGDYRNPLQAFRNVIGEKKKFRAHLLRRSTVFTQLMEDEATYDEPWIEPGEM
                     KFFIDGALGGSTALLSEPYADNPSTFGTAVLTDKEIDEHVATARKYGQAVAMHMIGDG
                     AVEKGLDAIERHPVPEGKRDRLIHVNVLRDDLVERMKKLPVVLDIQPVFVSSDFPWVM
                     ERLGEDRLDWAYAWKRLIDEGFICGAGSDAPIEEVDPLLGIYAATTRRKPGETHEGYL
                     PKENLSMFEAVSLFTSGSAATIGKADSRGKLGIGFDADFTVLDRDLFEADGEEILDTA
                     VVMTVVAGDIMYRK"
     gene            1792..>2146
                     /locus_tag="VFX34_06840"
     CDS             1792..>2146
                     /locus_tag="VFX34_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HEX5564659.1"
                     /translation="MRLTVYLAGEIHTSWREEVKQKVAALNLPIDFVGPMEDHDRSDN
                     IGEEILGEQPNAIFKDAAASSFNNLRTELLMKKADLVIALFGEQYKQWNTAMDASAAV
                     AYGKPLILIRSEQHHH"
BASE COUNT          508 a          607 c          427 g          604 t
ORIGIN      
        1 ttcttttttc acaatataaa actttctcct gtaaaacata gtatccctcc aaaaagtgaa
       61 aagaagataa cagaaaatca atcttctgtt atcttcctcc acaaatagct tttatcactt
      121 ccgatacatg atatcgcccg ctacaaccgt catgacaact gccgtatcca ggatttcctc
      181 cccatctgct tcaaacaaat ctctatctag caccgtaaaa tccgcatcaa aaccaatgcc
      241 gagctttccg cgcgaatctg ccttgccaat tgtcgccgcg cttcctgatg tgaataaact
      301 gaccgcctcg aacatgctta ggttttcttt cggcaaatac ccttcgtgcg tctctcccgg
      361 ttttctcctc gttgtcgccg cgtagatgcc tagcagcggg tcgacttctt cgatcggtgc
      421 atccgatcca gcaccacaga tgaacccttc gtcgatcaat cgcttccatg catatgccca
      481 gtccaggcga tcctccccta gacgttccat tacccatggg aaatcggatg atacgaaaac
      541 gggctgtatg tccaagacga ccggaagttt cttcattcgc tcaaccaagt catcccgcag
      601 cacattcaca tggatgagac ggtcgcgctt cccttccggc accggatgcc tttcgattgc
      661 gtccaaccct ttttcaacag cgccgtcgcc gatcatatgc atcgctactg cttgcccgta
      721 tttcctcgca gttgctacat gttcatcaat ctctttgtct gtcaacactg ctgtgccgaa
      781 agtagacgga ttatcagcat acggttcgct taacaaagca gtacttcctc caagcgcacc
      841 gtcgatgaag aatttcattt cccctggctc aatccatggt tcgtcatacg tcgcttcatc
      901 ctccatcagt tgggtaaaca ccgtcgacct ccgcaataaa tgcgcacgga actttttctt
      961 ttcgccgatg acattccgga atgcttgcaa tgggttgcgg taatccccgt aatagcccaa
     1021 gtcatccgtc acagctccag ttagtccttt cgagaataga tcgtcgaccg atttttccaa
     1081 tgccttcgtg agcatttcct ccgtcggctc cggaatgagc tgcagcacgc gatccatcgg
     1141 gccttccttc aagacacctg tcggctcgcc gttttcatca cgctcaatga caccgtcatc
     1201 cggatctggt gtatctttcg taatgccggc aagctccaac gctttcgaat tgacgattgc
     1261 cgcatgtctg cacgtccgtt tcaagtacat tggagaattc gtcactttat ccagttcata
     1321 acgtgtgaaa atctttttat ctgcaaaatt gttttcattc cacccttcgc cgatgaacca
     1381 ctcatcctct ttcaaatcag ggtaggcgtt cataagcata tccatcattt catccgaaga
     1441 cttcgctttc gacaagtcaa gactgagcaa tttctgaccg tggccaatca tatgcatatg
     1501 gttgtcaatg aaccccggat aaagcaccgc cccgtcaagg tcaacttcgt tttccgcttc
     1561 actcttcaat tcctcatatg tgcccatcgc gatgatcttt ccattttcag tcagtaacgc
     1621 ctcgacggtt tcgccttcct tctccatcgt atacagcttg ccgttatgcc aaatcgtttt
     1681 catcctgact cccccttttc tttccttaat tctaaacttt cgtgcaacgt tgggcaatct
     1741 aattcggagg aatgctataa tagggttatc attcttagga gggatttttt aatgagattg
     1801 acagtatatc ttgcggggga aatccatacg tcttggcgtg aagaggtgaa acagaaagtt
     1861 gcagctttga atttgccgat tgacttcgtt ggtccgatgg aagatcatga ccgctcggat
     1921 aatatcggag aggagatttt aggcgaacag cctaatgcca ttttcaagga tgcggccgct
     1981 tcgagtttca acaacttgcg gacggagcta ttaatgaaga aagccgatct tgtcatcgcc
     2041 ctcttcggcg agcaatacaa gcaatggaac acggcgatgg acgcaagtgc cgcggttgct
     2101 tacggcaaac cgcttatcct catccgttcc gagcagcatc accatc
//
LOCUS       DASULI010000320         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1760303,
            whole genome shotgun sequence.
ACCESSION   DASULI010000320 DASULI010000000
VERSION     DASULI010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760303"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFX34_06845"
     CDS             complement(<1..626)
                     /locus_tag="VFX34_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016716868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5564660.1"
                     /translation="MAAVAANGPGKKLNLFHLTWPIFLEIFLFMLMGLADTFMLSALS
                     DDAVSGVGAANQYVMIAILVLEVVGNGAAIVVSQYLGSRRFVEASKISALAVSLNLLV
                     GLGISGVFILFTSRMMNAMNLQGDVLAHAEAYLIIVGGGIFLQAVINSLAAVIRVHGF
                     TKQTMFVSLGMNIIHVVGNYALIFGKFGMPELGVQGAAISSVGSRVIA"
     gene            782..1879
                     /locus_tag="VFX34_06850"
     CDS             782..1879
                     /locus_tag="VFX34_06850"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEX5564661.1"
                     /translation="MKQITDELLSCIHQMTIENVSETRERVNQLFDFIELSRTLWGSV
                     PSITIPAELEKKEAEEETISTNETSQRMIVTSSADATNLGTPMERKLKGGILKGKHGD
                     VYVPEKIVRMHGFEHGDYIEAIGDRLKDLVFIKRKNSVTKHESNRIEIDYCVITKTSD
                     GLLVADHTIANGKLTRIKLDGESPYQFLISQRDSDTYSLKEGSVVTIAYHESDPLSFR
                     VVWCYPERTNEFARPEPKPSSFYKDPTPKKECIWTDVELNLLKDKKIVIIGADFRSSD
                     FMPLAESGNFTLEVFSGDESKTRLKAAVKNKDLIISASEHSSHRSSGLAKECAKKYKI
                     PFRAAPTSQSSIKRAMIHGIKESYIPFHSFF"
     gene            complement(2089..>3058)
                     /locus_tag="VFX34_06855"
     CDS             complement(2089..>3058)
                     /locus_tag="VFX34_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX5564662.1"
                     /translation="RMLSYRSVLDTYYPKGRSILAPLRVTYIFAGPRETVLHALIMKN
                     YGCTHALIGRDHAGIGDYYDKYASHSIFDQFKPEELGIDVRLFHEVFYCTRCDAPATE
                     QSCPHDTQYRINISGTGIREMLRHGIMPPKEIVRPESARIAIQGVQPKGLDEHGSAIS
                     PVGKVIKSMFPFYLERTRLGGRKRVRPLSVEELTNDDLEAVNLDVRANADRIYREVFE
                     EYTSVGDTNRGLQPEWVMDARDSLQTQQQMVISDLEEKVKQAPETASDEFMYQDKEEA
                     LRELEVAKKIFTDIPKVLRAADFDYRTWNTLPYKRYRGSDEPNEEK"
BASE COUNT          854 a          776 c          685 g          743 t
ORIGIN      
        1 attgcgatca cccggctgcc gaccgaagat atcgccgcgc cctgcacacc aagctccggc
       61 atcccgaact tcccgaagat gagcgcatag ttcccgacga cgtggatgat gttcatgcca
      121 agtgaaacga acatcgtctg ctttgtaaac ccgtgcaccc ggatgactgc ggcaagcgaa
      181 ttgatgactg cctgaaggaa aatccctccg ccgacaatga tcaaatacgc ttccgcatgg
      241 gccaggacat ccccttgcaa attcatcgca ttcatcatgc ggctcgtgaa tagaataaac
      301 acaccactaa ttcctaggcc gacgaggaga ttcaaggaaa cagctagcgc tgaaatcttt
      361 gatgcttcca caaaacgtct cgaacccaaa tactgcgaaa caacgatagc cgcgccgtta
      421 ccgactacct ccaagacgag gatggcaatc atcacgtatt ggttcgcagc accgacgccc
      481 gataccgcat catcggacaa cgcactcaac atgaacgtat ccgccaaccc catcaacatg
      541 aacaaaaata tctcaagaaa aatcggccac gtcagatgaa acaaattcag tttcttcccc
      601 gggccgttgg ctgcaactgc tgccatctat ttatcgccta catttctatt taagatcttc
      661 aaaacaggaa atagcttacc acagaaatca gggaaactac aatgggtttg cttcattttt
      721 ttaaactttt ttgtgtgcgg caatcaataa tttaataatt aaattccaag gaggtgagta
      781 catgaagcag attacggatg aactccttag ctgcatccat caaatgacca ttgaaaatgt
      841 atcggaaaca agggaacggg tcaatcagct gttcgacttc atcgaactgt cccggacatt
      901 atggggctcc gttccttcca tcacgattcc ggcggaactt gaaaagaagg aagctgagga
      961 agaaaccatt tcaacaaatg aaacatccca acggatgatt gtcacgagta gtgccgatgc
     1021 gacgaatctg ggaacgccaa tggaaaggaa attgaaaggc ggaatattga aagggaagca
     1081 tggggatgta tatgtcccgg aaaagattgt ccggatgcat gggtttgaac atggggatta
     1141 tattgaagcc atcggggatc gtctcaaaga tctggttttc atcaaacgga aaaatagtgt
     1201 gacgaagcat gaaagcaatc gaattgaaat cgattattgt gtcatcacaa aaacatcaga
     1261 tggattatta gttgcagatc atacgatcgc aaacggaaaa ttaacgagaa taaaacttga
     1321 cggcgaatcc ccttatcaat ttttaattag ccaaagagat agtgatactt atagtttaaa
     1381 ggaaggaagc gtcgtgacga ttgcctatca cgaatcagat cccctatcgt tccgtgtcgt
     1441 ctggtgctat ccggagcgca caaatgaatt tgctagaccc gaaccgaaac cgtccagttt
     1501 ttataaagat cctactccga aaaaagaatg catatggacc gatgttgaat tgaatttatt
     1561 aaaagataaa aaaatcgtta ttatcggggc cgatttccgg agttctgatt tcatgcccct
     1621 agcggaaagc gggaatttca ctttggaggt gttcagtggg gatgaaagca aaacccgtct
     1681 gaaagcagct gtcaaaaata aagatttgat catttccgca agcgagcatt cctctcatcg
     1741 cagcagcgga ttggccaagg aatgtgccaa gaagtacaaa atcccattcc gcgcagcccc
     1801 tactagccaa tcatcgataa aacgagcgat gattcacggc atcaaggaat cgtatatccc
     1861 atttcattca tttttctaaa ggggggtgct ttaatcgaaa aaagctttcc gccaagaagg
     1921 cggaaagctc tccgttaaaa gttatgctcg agtttacata cgttatggag caattcggcg
     1981 tgagtttggc gcaatctagc caagttatgc tcgagttccg ttgagttatg gcgcaatctt
     2041 aacgagttat gctcgtcttg ggactttcca cccgtccgga acaataactt atttctcctc
     2101 attcggctca tcgctgccgc ggtagcgctt atacggcaag gtattccatg tccggtagtc
     2161 gaaatcggcc gcgcggagca ctttcggaat atcggtaaag atcttcttcg ccacttccaa
     2221 ttcccgcagt gcttcctctt tatcctgata catgaattca tccgatgcgg tctccggcgc
     2281 ttgcttcact ttctcctcaa gatctgagat gaccatctgc tgctgggttt gcaatgaatc
     2341 cctcgcatcc atcacccact ccggctgcaa gcccctgttc gtgtcaccga cactcgtata
     2401 ttcctcgaaa acttcccggt agatccggtc ggcattcgcg cggacatcca gattgaccgc
     2461 ttccaaatca tcattcgtca attcttcgac cgacaaaggt ctaacccgtt tccggcctcc
     2521 gagcctcgta cgctctaaat agaacgggaa catgctttta attaccttcc cgacaggcga
     2581 gatagcgctt ccatgttcat caagcccttt tggctggacg ccttggatgg caatccgcgc
     2641 tgattccgga cggacgatct ccttcggcgg catgatgccg tggcggagca tttcccggat
     2701 tcccgtacct gaaatattga tccggtattg agtatcatgc ggacaggact gctctgtcgc
     2761 aggcgcgtca caacgcgtgc aatagaacac ttcatggaac agccgcacgt caatgccgag
     2821 ctcttccggc ttgaactgat caaaaatgct atggctcgca tacttatcgt aataatctcc
     2881 gattcctgca tgatcccggc cgatgagtgc atgcgtacag ccatagttct tcatgatcaa
     2941 tgcatgcagc accgtctccc gtggaccggc aaagatatac gtcaccctga gtggcgcaag
     3001 gatggacctc cctttcggat aatacgtgtc cagcacgcta cgatacgaca gcatccgg
//
LOCUS       DASULI010000321         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1312924,
            whole genome shotgun sequence.
ACCESSION   DASULI010000321 DASULI010000000
VERSION     DASULI010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312924"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFX34_06860"
     CDS             complement(<1..632)
                     /locus_tag="VFX34_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5564663.1"
                     /translation="MRKLVLLGAGYGNMRILLRLLNKELPANLEITLIDRTPFHSLKT
                     EFYALAAGTAPDSDIRVALPTHENLKVVEGELAKIKLEDKAIALKNGEEISYDELVIG
                     LGCEDNYHDVPGAAEYTSSIQTIGQSRATYKLLQGLGGGATVGIVGAGLSGIELASEL
                     RESRPDLKIKLFDRGPRILKDFPERLSNYVKDWFDKHEVEVISNSNITRV"
     gene            771..1007
                     /locus_tag="VFX34_06865"
     CDS             771..1007
                     /locus_tag="VFX34_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HEX5564664.1"
                     /translation="MNPIVEFCMSNLANGSQKTLEALERDPNIDVLEYGCLSYCTKCA
                     EGLYALVNGEVVEADTPEELTERIYQYIEENPLF"
     gene            complement(1029..1802)
                     /locus_tag="VFX34_06870"
     CDS             complement(1029..1802)
                     /locus_tag="VFX34_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEX5564665.1"
                     /translation="MDRYKAVCFDLDGTVYRGNEVIPEAAALIEGLQRGGIEPYYITN
                     NSSGTAEQFQMKLLHMGIKAEADHIMTSAIASAKYCKEHFNGASVMMIGEEGLEEALV
                     SEGFTLTSQHPDVVVVGIDRGITYEKLAAASLAIRAGAVFLATNGDRAFPTEKGLVPG
                     NGSFVKLLESTTDRNAIYVGKPEPHMLHFIQQKGYRKEEMIMIGDNYETDIQAGIRFG
                     IDTIHVEGGVTSLETVMEQLERPTYLFKSLMDWNKKEPQ"
     gene            complement(1805..2254)
                     /locus_tag="VFX34_06875"
     CDS             complement(1805..2254)
                     /locus_tag="VFX34_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEX5564666.1"
                     /translation="MYFINRQKINETLAYMESLLALFEGHQKWNESKIHALALERIAH
                     TIVESIIDVGNSMIDGFIMRDPGSYDDIIDIMEDEKVISSEMADPLKKVIGLRKMIVR
                     EFVDVDNEEVEAVLNEAKEEIKQFPIKVRHYLENELGPVSAFSPLEQ"
BASE COUNT          589 a          561 c          472 g          715 t
ORIGIN      
        1 tcgactctcg ttatgttcga gtttgaaatg acttctacct catgtttgtc gaaccaatct
       61 ttgacgtagt tgctgagtcg ttctgggaag tcctttagga ttcgtggacc tctgtcgaat
      121 aatttgattt tcaaatccgg ccgactttcc cgcaattcac ttgccaactc gattccgctc
      181 aatccagcgc cgacgatgcc gactgtcgca ccgccgccaa gaccttgaag cagtttgtat
      241 gtggcgcgcg attggccgat tgtctggatg ctgcttgtgt attccgctgc tcccggtacg
      301 tcatggtagt tatcctcaca tccgagtccg atgacaagct cgtcgtaaga aatctcttca
      361 ccgtttttga gggcgatcgc tttgtcttcc aatttaattt tcgcgagttc cccttctaca
      421 accttaagat tttcatgtgt aggtaatgcg acacggatgt ccgagtctgg tgccgttccc
      481 gctgctaatg cataaaattc tgttttaaga ctatggaaag gtgttctatc aatcaatgtg
      541 atttccaaat tagcgggcaa ttctttattg agtagacgaa gcaaaattcg catattaccg
      601 tagcctgcac ctaataacac tagttttctc atagttttct cctttgccaa tcgttttccg
      661 atttattccc atcatgatta taacagtttg tgacatgttt cacaataaga catcctattt
      721 gactgcaggc gaaaataaga gtagcatgcc aagtaggtga ggtgatcgtc gtgaatccga
      781 tcgtagaatt ctgtatgagc aatcttgcta acggttcaca gaagacattg gaagcgttgg
      841 aaagggatcc aaatatagat gtgttggaat atggctgtct cagttattgc acaaaatgtg
      901 cagaaggatt atatgccctc gtcaatggtg aggtcgtcga agcggacaca ccagaagagc
      961 tgacggagcg gatttatcaa tatattgaag agaatcctct tttttaagac agcaaagaac
     1021 cgtaattttc attgcggttc ttttttattc caatccatta ggcttttgaa tagatacgtc
     1081 ggccgttcca attgctccat cactgtctcc aaacttgtca cgccgccttc tacatgaatc
     1141 gtatcgattc caaagcggat tcccgcctgg atgtctgttt cataattgtc accgatcatg
     1201 atcatttctt ctttcctata ccctttttgc tgaatgaaat gcagcatatg cggttcaggc
     1261 ttgccgacat agatggcatt gcgatccgtt gtcgactcca agagctttac aaatgaaccg
     1321 tttcccggaa cgagcccttt ttcggtcgga aacgctcgat ccccattcgt tgctagaaaa
     1381 actgcacctg cacggattgc caaacttgct gctgcaagtt tttcatatgt aatgccgcgg
     1441 tcgattccga caacgacgac gtccggatgt tgtgatgtca gcgtgaatcc ttctgaaaca
     1501 agtgcttctt cgagaccttc ttctccaatc atcatgacgg atgcaccgtt gaaatgctct
     1561 ttgcaatatt tcgcggatgc gattgccgag gtcatgatat gatccgcttc agccttgatc
     1621 cccatgtgaa gaagtttcat ttgaaattgc tcagccgtgc cggaagagtt attcgttata
     1681 taataaggtt caatgcctcc acgctgaagt ccttcaatca atgcagcagc ttccggtatc
     1741 acttcatttc ctcgatatac ggtcccatcc aaatcaaaac aaaccgcttt atagcgatcc
     1801 attgttattg ctccaatgga ctgaaggcag aaacaggacc aagttcattc tccaagtaat
     1861 gcctaacttt tatcgggaat tgtttaattt cctctttcgc ttcattcaaa accgcttcaa
     1921 cttcctcgtt gtccacatcc acaaattcac gaacaatcat tttacgaagt ccgatcactt
     1981 ttttcaacgg gtccgccatt tccgaggaaa tcaccttttc atcttccatg atgtcgataa
     2041 tatcgtcata actccccgga tcacgcataa tgaatccgtc gatcatggaa ttgccgacat
     2101 cgatgattga ctcgacgatg gtatgggcaa tcctttcaag tgccaaagcg tggattttgc
     2161 tttcattcca tttttgatgc ccttcgaata aagcaagaag gctttccata taagcaagtg
     2221 tttcattgat tttttggcga ttgataaagt acaaaactgt tccctcccgg ttgtctaaac
     2281 tcctatccat catatcataa tgaacacacg aatgaaccgc cctgtacacg gtatccg
//
LOCUS       DASULI010000322         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1819416,
            whole genome shotgun sequence.
ACCESSION   DASULI010000322 DASULI010000000
VERSION     DASULI010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819416"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..326
                     /locus_tag="VFX34_06880"
     CDS             54..326
                     /locus_tag="VFX34_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEX5564667.1"
                     /translation="MNSYDKTMKFNFPEESMEQEVKQVMLHVHKALEDKGYNPINQIV
                     GYLLSGDPAYIPRHYEARNLIRKLERDEILEELVKFYIRENGGKTN"
     gene            323..742
                     /gene="ruvX"
                     /locus_tag="VFX34_06885"
     CDS             323..742
                     /gene="ruvX"
                     /locus_tag="VFX34_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5564668.1"
                     /translation="MRVMGLDVGSKTVGIAISDALGWTAQGIETIKIDENAGQFGMSR
                     IQELVSEYAVTSFVVGYPKNMNNSVGPRGEASEKFADMLKEAFGLPVSLWDERLTTMA
                     AERMLIEADVSRKKRKTVIDKMAAVMILQGYLDSKNR"
     gene            754..1050
                     /locus_tag="VFX34_06890"
     CDS             754..1050
                     /locus_tag="VFX34_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020754570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEX5564669.1"
                     /translation="MEHGQETMTVVDEHGEEHVCEVIFTFESEDFGKSYVLYHILGEE
                     EVGDDEEVEIHASAFIPSEDNEDGELLPIETDEEWDMIEEMLNTFLAEEDEDEE"
     gene            1150..>2080
                     /gene="mltG"
                     /locus_tag="VFX34_06895"
     CDS             1150..>2080
                     /gene="mltG"
                     /locus_tag="VFX34_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5564670.1"
                     /translation="MDNGSKKDKMFEKMLEQKKEVKIVRRIVFVVALVLIISGLIGGR
                     AVYKYISEALQPVDPNSEEVIEVEIPIGSGLTSISKMLEEKGLIKDAKIFKYYAKFNN
                     ESQFQAGSYGLTKAMTLDELIESLKTGKVYRTPVFTMTIPEGLTLEKIAAIVEKRTGI
                     KEKEFLDYVNDEQTIADLMGRYPNILTEEILGENVRYPLEGYLFPATYPFYEEKPTVE
                     TVVTTMLDATSDNITPYFAYLESNEKSVHWLLTFASLLEREATSNTDRETIASVFYNR
                     MDEGMPLQTDPTVLYALGEHKDRVLLSDLKVEDP"
BASE COUNT          647 a          376 c          545 g          512 t
ORIGIN      
        1 gtgggaaagg tatccgattt gcaaacggac gattggaaag cggggtgtcg actatgaatt
       61 catacgacaa gacgatgaaa ttcaactttc ctgaagaatc gatggagcaa gaagtgaaac
      121 aggtcatgct ccatgtgcac aaagcactgg aagataaggg ctacaatccc atcaaccaaa
      181 ttgtcggtta cctcttatcg ggcgacccgg cctatattcc tcgccattat gaggcgcgta
      241 accttatccg gaaattagag agggatgaaa tcctcgagga attggtgaag ttttatatcc
      301 gtgaaaacgg aggtaagacg aattgagggt aatgggattg gacgtcggct ccaaaacggt
      361 cggtatagca atcagcgatg cacttgggtg gacagcccaa ggcatcgaaa cgattaaaat
      421 cgatgaaaat gccggccaat ttggcatgtc cagaattcag gaactcgtta gtgaatatgc
      481 ggtcacctct ttcgtggtcg gatatccgaa aaacatgaat aactccgttg gtccgagagg
      541 ggaagcatcc gaaaaattcg ctgacatgtt aaaagaggcg ttcggccttc ccgtaagctt
      601 atgggatgag cgtttaacga cgatggctgc agaacgcatg ttgatcgaag ctgatgtcag
      661 caggaagaaa aggaagacgg tcatcgataa gatggctgca gtcatgatac ttcaagggta
      721 tcttgattca aaaaatagat gaggtgatta acaatggaac acggtcaaga aacaatgaca
      781 gtcgttgacg agcacggtga agagcatgta tgtgaggtta ttttcacatt cgagtcggag
      841 gatttcggaa aatcatacgt tctttatcat attttaggcg aagaagaagt gggcgatgat
      901 gaagaggtcg aaattcatgc ttctgcattc attccatccg aagacaacga agacggtgaa
      961 ctattgccga tcgaaactga cgaggaatgg gatatgattg aagagatgtt gaacacattc
     1021 cttgcagaag aagacgagga cgaagaataa tccatatgtg cacggacgga atggtgcagg
     1081 ccattccgtc ttttcactgc aatggatcct ttttatagat agtgaatgtt cgaaatgagg
     1141 tgatgctgga tggataatgg ttcaaaaaag gataaaatgt tcgaaaagat gcttgaacag
     1201 aaaaaagaag tgaagattgt caggcgtatc gtgttcgttg ttgcgctcgt tcttattatc
     1261 agcggcctga tcggaggtcg agcggtctat aaatacataa gtgaggcact tcaacccgtc
     1321 gacccgaact ctgaagaagt gattgaagtg gagatcccga tcggttcggg cttgacatcg
     1381 atttcgaaaa tgttggaaga gaaaggtctc atcaaagatg cgaagatatt taaatactac
     1441 gctaaattca acaatgaatc acaatttcag gccggttcct atggtttgac aaaggcgatg
     1501 acattagatg aattgattga aagcttgaag acggggaaag tttataggac tcctgtattc
     1561 acgatgacga ttccggaagg cctgacgctg gaaaagattg cagcaatcgt cgaaaagcgg
     1621 acaggtatta aggaaaaaga gtttctggac tatgtcaatg atgagcagac cattgcagat
     1681 ctgatgggaa gatatccgaa catcttaacc gaagagatat tgggagagaa tgtcagatat
     1741 ccattggaag ggtatttatt cccggcaacg tatccattct atgaggaaaa gccgacagtc
     1801 gaaacggttg taacgacaat gctggatgca acttcggata acatcacccc ttatttcgcc
     1861 tatttggaaa gtaatgagaa atctgtccat tggctgctca cttttgcaag tctgcttgaa
     1921 agggaagcga cttcaaatac cgatcgagaa acgattgcaa gtgtctttta caatcgtatg
     1981 gatgagggaa tgccattgca gacggatcct actgttttgt atgcattagg cgaacataag
     2041 gatcgggtcc ttttatccga cttgaaagtc gaggatccgt
//
LOCUS       DASULI010000323         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1138235,
            whole genome shotgun sequence.
ACCESSION   DASULI010000323 DASULI010000000
VERSION     DASULI010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138235"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..740
                     /gene="lepB"
                     /locus_tag="VFX34_06900"
     CDS             180..740
                     /gene="lepB"
                     /locus_tag="VFX34_06900"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5564671.1"
                     /translation="MMNEEKKKNETWEWIKALLIAFGLAAIIRIFLFTPIVVDGISMM
                     PTLEHGDKMIVNKIGYTLGEPDRFDIVVFHAPEKKDYIKRVIGLPGDTVEYRDDVLYI
                     NDQPYDEPYLDQYKAEIAEGTLTEDFTLEEKIGMRTVPEGHVFVMGDNRRKSKDSRHI
                     GAVPIDEIIGSTKFIFWPISDFGVVE"
     gene            760..1638
                     /gene="ylqF"
                     /locus_tag="VFX34_06905"
     CDS             760..1638
                     /gene="ylqF"
                     /locus_tag="VFX34_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEX5564672.1"
                     /translation="MTIQWFPGHMAKARREVTEQLKLVDIVFELIDARLPLSSRNPMI
                     DEVIQQKPRLLILNKMDLADEKETERWIRHFETEGIRAVAINSFEGKGLQTVTKAAKE
                     ILAPKIERLKKRGIRPGAIRAMIVGIPNVGKSTLINRLARKNIAKTGNKPGVTKAQQW
                     IKFEKELELLDTPGILWPKFEDQAVGYKLALTGAIKDTIVNMENLAVYGLRFLEEHYP
                     DRLEKRYGISTVGEDIQKVFDVIGESRKAYTQGGEIDYDKVAELIVQDIRSELLGKLT
                     FDFTSDQSKQADAVTE"
     gene            1916..2686
                     /locus_tag="VFX34_06910"
     CDS             1916..2686
                     /locus_tag="VFX34_06910"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5564673.1"
                     /translation="MKTIKEISFRLQQTLNPEPWMKELEDDHRAGVKTELQRWYRNYE
                     KKQKLEQMLREKINFDNSFKTTSQSLLAGVDEAGRGPLAGPVVCAAVILPDEAPQLLG
                     LNDSKMISLQERNRFSTIIKEIAISYSIHIQAAEKIDEMNIYAATRQSMETAVNSLDI
                     LPHIVLADAMKLQVNCRTESIIKGDAQSLAIAAASILAKTTRDQLMNEIHEEFPMYNF
                     KKNVGYGTGEHVEALRIHGPCEHHRKTFEPVKSILEGM"
     gene            2693..>3050
                     /locus_tag="VFX34_06915"
     CDS             2693..>3050
                     /locus_tag="VFX34_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564674.1"
                     /translation="MNYPSIPRLSNQPMPVSQQPMLMREGQMFHGQIKQLFPGQMAEV
                     QIGGQKLMAKLEVPMKAGDAYYFQVKSVTPELQIKVVSGPLQSTEGQAKQMANLMESL
                     QLPKSPEMMAIMNHFLK"
BASE COUNT          970 a          676 c          731 g          673 t
ORIGIN      
        1 cgaacccttc gctgttcgat tgtcttgggt cataagtcag ctctgctata cggcaaagtg
       61 cgccgtttcg cagacccgcc ttatgcctgt cgactctttt caggcgccct tcgcatttgt
      121 tctttgcctc ataattgaat agactgtaca ataagagtag cgacttggca ggaggctttg
      181 tgatgaatga agagaaaaag aaaaatgaaa cctgggaatg gatcaaagcg cttttaattg
      241 cgtttggatt agcagcaatc atccggatat ttttattcac accgattgtt gtggacggca
      301 tttcaatgat gcccacactc gaacacggcg ataaaatgat cgtcaataaa atcggctaca
      361 cactcggtga acctgaccgt tttgatattg ttgttttcca tgcaccggaa aaaaaggact
      421 acatcaaaag ggttatcggc cttccggggg atacagtgga atatcgggat gatgtcctat
      481 acatcaacga tcaaccttat gacgaaccat atcttgatca atataaagcc gaaattgccg
      541 aaggaacttt gaccgaagat tttaccttgg aagaaaaaat cgggatgcgg accgttcccg
      601 aaggccatgt atttgtaatg ggcgacaacc gccgaaaaag taaagattca aggcatatcg
      661 gggcagtgcc cattgatgaa atcataggca gcacaaaatt cattttctgg ccgatcagtg
      721 attttggcgt ggtggagtga aggaaggggt atgtaatcaa tgaccattca atggtttccg
      781 ggccatatgg ccaaagcaag acgtgaagtg acagaacaat tgaagctcgt cgacatcgta
      841 ttcgagttga ttgacgcccg attgcccctc tcatctagaa atccgatgat cgacgaagtc
      901 attcagcaaa aaccgagatt gctcattttg aataaaatgg accttgccga tgaaaaagag
      961 acggagcgtt ggatccgaca cttcgagacg gaaggcatcc gggctgtcgc cattaattca
     1021 ttcgaaggaa aagggctgca gaccgtaaca aaggcggcaa aggaaatatt agcaccgaaa
     1081 atcgaacgtt tgaaaaagag agggatccgg ccgggggcaa tccgtgcaat gattgtgggg
     1141 attccgaatg tcgggaagtc gactttgatc aaccgactcg caaggaaaaa tattgcaaaa
     1201 accggcaaca agccaggcgt caccaaagcg cagcaatgga tcaaattcga gaaggaactg
     1261 gaattgctcg atacaccggg gatcctttgg ccaaaatttg aagaccaagc tgtcgggtat
     1321 aagcttgcgc tgacaggggc catcaaggat acgatcgtca acatggaaaa cctggcagtc
     1381 tacggcctcc gattcctgga agaacattat ccggaccggc tcgagaagcg atacgggatc
     1441 tcaaccgtag gcgaagatat ccaaaaggta tttgatgtaa tcggcgagtc ccgaaaagcg
     1501 tatacacaag gcggcgaaat cgattatgac aaagtcgctg aactgattgt ccaagacatc
     1561 cgcagcgagc tgcttggcaa gctgacgttc gatttcacat ccgatcaatc caaacaggcc
     1621 gatgcagtca cggaataacc tccgtgacaa tcggcctttt cctattgaaa caccgtgaag
     1681 attcatccga tataaaggga gagtacggag tttagttctt aagcaaggat tgaagcgaaa
     1741 ggcggcgact cctgtgaacg ccgtcacaaa gaacggcgtt tgcgagcagg ctgcctcaaa
     1801 ttctgcaaag tacgcagaaa tacggcaaat cgaacccttc gtagtacgat tggaaagcgt
     1861 ccgcctgtag cggaaatcca tttatcaatc tcatacataa tagaaggtga aaaaaatgaa
     1921 aacaatcaaa gaaatttcat ttcgtctgca gcaaacttta aaccccgaac catggatgaa
     1981 agaacttgaa gacgatcatc gtgcaggcgt caaaacagaa ttgcaaagat ggtatcgcaa
     2041 ttacgaaaag aaacaaaaac tggaacaaat gctgcgagaa aaaatcaatt tcgacaatag
     2101 ctttaaaaca accagccaat cactcttagc aggtgtcgat gaagcaggaa gagggccgct
     2161 cgcagggcct gtcgtctgcg cagctgtcat attgcctgat gaagcaccac agctgctcgg
     2221 actcaatgat tcaaaaatga tatcattaca agaaagaaat cgattctcta ctattataaa
     2281 agaaatcgca atcagttact caatacatat ccaagccgca gaaaaaattg acgaaatgaa
     2341 catctacgcg gcgactagac agtcgatgga aacggcagtg aacagcttag acatccttcc
     2401 gcatatcgtc ttggcggatg ccatgaaact tcaggtgaac tgcaggacag aatcaatcat
     2461 caagggagac gctcaaagtc tagcgatagc ggcagcctct atccttgcga aaacgaccag
     2521 agaccaattg atgaacgaaa ttcatgaaga atttccaatg tacaacttca agaaaaacgt
     2581 cggttacggc actggagagc atgtggaagc actccgcatc catgggccat gcgagcacca
     2641 cagaaaaaca tttgagcctg tcaaatcgat tttggaagga atgtgatccg acatgaatta
     2701 cccatcgatt ccccgattga gcaatcagcc catgcctgta tcacaacagc ctatgctaat
     2761 gcgggaaggc caaatgttcc atgggcagat aaaacagctt tttccgggac agatggcaga
     2821 ggtgcaaatc ggtggccaaa aattaatggc caaactggaa gtccctatga aggcagggga
     2881 tgcctattac ttccaagtga aatctgtcac tcccgagctg caaatcaaag tcgtttccgg
     2941 accattgcaa tcgacggaag gccaagcgaa gcaaatggcg aatttgatgg agtcgcttca
     3001 acttccgaaa tctcctgaga tgatggctat aatgaatcat ttcttgaaag
//
LOCUS       DASULI010000324         6260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_709990, whole
            genome shotgun sequence.
ACCESSION   DASULI010000324 DASULI010000000
VERSION     DASULI010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709990"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..475
                     /locus_tag="VFX34_06920"
     CDS             47..475
                     /locus_tag="VFX34_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5564675.1"
                     /translation="MKTNGVIFIIAFLLAFGGGYLYFSQGGKDQKQQTTQQGASTETG
                     KEQVEKEEVATVPAEAEVMSNNSCLSCHAVSSMGVEGGTTGPDLSNAYKDIEGKHGKD
                     LDSFLQEPTSAVMSGVIGGSPLSEEDRAEIVKFLEKASEQ"
     gene            506..2368
                     /gene="nosZ"
                     /locus_tag="VFX34_06925"
     CDS             506..2368
                     /gene="nosZ"
                     /locus_tag="VFX34_06925"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011887499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEX5564676.1"
                     /translation="MMKRIIAAVVGIAAGLLVAVVFFADLNKADQNAGENKSKAEKVY
                     VPYGEKDEYYMMASGGHSGQLFVYGLPSMRKIRTVPVFTPDPATGYGFDEKTKTMLGG
                     YTWGDFHHPSISETEGEYDGQFLFANDVGNNRVAAVDLSTFHTVDIMSLPNMGGPHAG
                     VFVTENTEYIALPTRFARPLDGQFADLDEYENEYKGVVSMTTFDRETKKFDLAYQVVL
                     PPWSYDLSDAGKKMSSDWIVMTTYNSEFATTNLEINASQNDRDYIVLFNWKKLEEEVA
                     AGNYDDVNGAKMIDPRKHKGAIFAVPVAKSPHGVDVTPDGTRFIASGKLAPMMTVFSF
                     QKAFDAIEKGSFTDEKFGIPVLAYEDVMEREVDPPGALGPLHTQFDDRGNAFNTMFIS
                     SEVVKWNIETGEVIDRTPTYFSPGHSAATEGDTVSPDGKWLVALNKIAKDNFLSVGPS
                     HPESMDLIDISGDKMEHIMAVPVDPEPHYAQIIHRDKIKTITVYPKDEARENAVWKQE
                     DTRIERKGNEVHVYGIAMRSKFIFDSKSERPDAVTVKEGDRVFFHMTNIDLDQDITHG
                     FGINDYDLNFEVQPGQTNTLEFVADKAGVYPIYCTNFCSALHQEMSGYLLVEPK"
     gene            2451..3017
                     /locus_tag="VFX34_06930"
     CDS             2451..3017
                     /locus_tag="VFX34_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564677.1"
                     /translation="MKGRLSIASMISLIIASIITVFTLFVPWWGMKFYAPQYPEGLDI
                     IVYPTTLDGDIDIVNGLNHYIGMAEFNNESFPELQWMPYIVVGFAVLILLVALFRRKK
                     FLYGLIAVFVAGGIVGLWDMYRWLKDFGMNLDPRAPIDLEPFIPPIIGKNIIANFTTI
                     SYFTYGGYMLLLVFILVVYPLWKDRKKP"
     gene            3014..4318
                     /gene="nosD"
                     /locus_tag="VFX34_06935"
     CDS             3014..4318
                     /gene="nosD"
                     /locus_tag="VFX34_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEX5564678.1"
                     /translation="MKKIIYLSFLSFLFLTGQADAQDVGNLQQLIDETPIGGVLELED
                     ATYEGNVVLTKPITIKGKKGTRIKGDKTVNVIEVRSKDVTLDTLSIEGSGMSRSSQEE
                     YSAIRVMADGAVMRNLHIADSFHGIYLNKTKNTVISHVKIVGQGTDAVGNQGNGVHIA
                     RSSDNHIDNCDIEKTRDGIFVEYSNNNVIIDNTMSQTRYGLHYMYSNNNEFKRNNFVN
                     NVGGAAIMHSDHIILADNKFSFNQGSRSFGLIVQTSRDVHVLNNEFHLNQRGLYLEQS
                     TSNVIEGNDFFQNQIGVELWTSSTAHVFVKNNFRKNRIHALTVGGESNNEWFRNGEGN
                     YWNVPMLDFNRNGIGDAPMEYTSALGELVETNELSYLFLSSPAITIYEKANELMTFQK
                     VMAYDKYPLMNSQKYRGHEWALLAAVLVLAGWLVYRKKAKKR"
     gene            4315..5115
                     /locus_tag="VFX34_06940"
     CDS             4315..5115
                     /locus_tag="VFX34_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5564679.1"
                     /translation="MMYVWKEFLEQTRGKGLWLGIGVLMLATLFLIAEARSFPADLGF
                     EAMLLSVFDMNMYLLPLFAMFLSSFSIFQEKELKTSMILLTKRESTRSLLFKKSVAIQ
                     VVLLSIFLVAYFILALFMKVFLAFHVVSFLHFLLVITVFLLIFNQLGIFLGVACNTKM
                     QLVGANILTWFFVVFLFDLMFLYFLPAVTFDNLHFFSWVYFLDPIHTLRFYLETSLGL
                     FSLSHMSRMMENFVFMDMKYFLAINVLLWPGLLFGLSTLFRNGGTIDD"
     gene            5108..5776
                     /locus_tag="VFX34_06945"
     CDS             5108..5776
                     /locus_tag="VFX34_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019154060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564680.1"
                     /translation="MIKIQNVSHAYHKKKVLDDVSLHIGANERCALVGRNGAGKSTMI
                     HVMLGLLPIQKGSIVLGGIPNKKQEWKRIVSYLPEKFHLYPHLTGEENMRFFASLSTK
                     KIDEESIAEKLKMVSLYEARGEQITKYSKGMLQRLGLAVMLYYDSDIIILDEPTSGLD
                     PMGREEILQIINSLDNKTILMASHHLSEIEKVCTHVAFLEDGKVIKYPVDEFIRTHLK
                     EESK"
     gene            5773..6132
                     /locus_tag="VFX34_06950"
     CDS             5773..6132
                     /locus_tag="VFX34_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX5564681.1"
                     /translation="MKRLVVSLLLIVVLAAGCSSAKMDISIEKMPESKTGTPSEMLIK
                     VAGDGVGLEGLSVVASLEMAKMDHGELEVQFEEIGDGLYSGQVELPMGGEWIADVEVK
                     SGEETVKEILTVEVEER"
BASE COUNT         1739 a         1298 c         1620 g         1603 t
ORIGIN      
        1 attataaata catgttatta aaaaaagaaa gaggaggtag cagaaaatga aaacaaatgg
       61 tgtgatcttt attatcgcat tcctattggc tttcggagga gggtacttgt atttcagcca
      121 aggaggtaaa gatcaaaaac agcagactac acaacagggc gcttcaaccg aaacgggcaa
      181 ggagcaagtg gaaaaggaag aagtcgcaac cgtcccagcc gaagcggaag tcatgagtaa
      241 caatagctgt ttgtcatgtc acgctgtcag ctcgatgggt gttgaaggag gaacaacggg
      301 tcccgatctc tcaaatgcat ataaggatat cgaagggaaa cacgggaaag atttagactc
      361 gttcttacaa gaaccgacat ccgcggtcat gtcgggcgtc attggcggca gtccattatc
      421 ggaagaagat cgtgctgaaa ttgtaaagtt tttagaaaaa gcatcagaac aataaagagc
      481 ggggtgaaga gaattgaata caccattgat gaaacggatt attgcagcag tcgttgggat
      541 agcggcaggt cttttggtag ctgttgtgtt cttcgcggat ttgaataaag cggatcaaaa
      601 tgcaggtgaa aataagtcga aggctgaaaa agtgtatgtc ccttatggag aaaaagatga
      661 gtactatatg atggcttccg gcgggcattc aggacaatta ttcgtctatg gccttccgtc
      721 catgcgtaaa atccggacgg tgcccgtttt tacgccggat ccagccacag gatatggatt
      781 tgacgaaaag acgaaaacga tgttaggcgg ctatacatgg ggggatttcc atcatccttc
      841 catttcggaa acagagggtg aatatgacgg acagttcctc ttcgcgaatg acgtagggaa
      901 caaccgcgtg gcggcagtgg atttgagcac tttccatact gtggacatca tgtccttgcc
      961 gaatatgggc ggtccccacg ccggtgtgtt tgtgactgaa aatacggaat atatcgcgct
     1021 gccgacccgc tttgcccgtc cgttggatgg acagttcgcg gacttggatg aatatgaaaa
     1081 tgaatacaaa ggcgtcgtct ccatgacaac ttttgatcgt gagacaaaga aattcgacct
     1141 tgcctatcaa gtcgttcttc cgccttggtc ttatgacttg tccgatgccg gaaagaagat
     1201 gtcctccgac tggatcgtca tgacaacgta caattcagag tttgcgacaa cgaatctgga
     1261 aatcaatgcg tcacaaaatg accgagacta tatcgttcta ttcaactgga aaaagcttga
     1321 ggaggaagtg gcggcaggta attacgatga tgtgaacggc gctaaaatga ttgacccgcg
     1381 caagcataaa ggggcgatat tcgcagttcc tgtcgcgaaa tcaccgcatg gcgtcgatgt
     1441 tacgcctgac ggcacacgat tcattgcttc aggtaaactt gcgccgatga tgacagtctt
     1501 ttcattccaa aaagcattcg atgcgattga gaaaggcagt ttcacggacg agaaattcgg
     1561 catcccggtg ctcgcctatg aagacgtcat ggagcgtgaa gtcgatcctc cgggtgcgtt
     1621 aggacctctc catacccaat tcgatgatcg cgggaatgca tttaatacga tgttcatttc
     1681 atctgaagtc gtgaaatgga atattgaaac gggcgaagtc attgaccgta cgccaaccta
     1741 cttctcgccg ggccactccg ctgcaaccga aggggatact gtatcacctg acggcaaatg
     1801 gctcgttgca ctgaacaaga tcgccaaaga caatttccta tctgtcgggc catcccatcc
     1861 ggagtcgatg gacctcatcg atatttccgg ggataagatg gaacatatca tggcagttcc
     1921 tgtcgatccg gaaccgcact atgcgcaaat catccatcgg gataagatca aaacgataac
     1981 ggtctatccg aaagacgaag cccgggaaaa tgcagtttgg aaacaggaag atacacgtat
     2041 tgaacgtaaa gggaatgaag tgcatgtata cggcatcgcg atgcggtcga aattcatctt
     2101 cgactcgaaa tcggaacgtc cggatgccgt cacagtcaaa gaaggcgaca gggtgttctt
     2161 ccatatgacg aatatcgatt tggaccaaga tattacgcac ggcttcggca tcaacgacta
     2221 cgacctgaat ttcgaagtgc agcctggcca gacgaacaca ttggaattcg tcgctgacaa
     2281 agccggcgtt tacccgatct actgcacaaa cttctgttca gcgcttcacc aggaaatgag
     2341 cggatattta ttagtggaac caaaataaca gcatgaatga agaggtgaaa ggcctgaaag
     2401 atgaggaaca aacctttcac ttctttccaa ccatttggag gtgagctcct atgaagggga
     2461 gactttccat cgcttcaatg atcagtttaa tcatagcctc gatcatcacg gttttcactt
     2521 tgttcgttcc gtggtggggc atgaaattct acgcgccgca atatccggag gggctggata
     2581 tcatcgtata tccgacaaca ttggacgggg atatcgatat cgtcaatggt ctcaaccatt
     2641 atatcgggat ggcggaattc aacaatgaat cattccctga attgcaatgg atgccatata
     2701 tcgtcgtcgg ttttgccgtc cttatcctct tagttgcgct gttccggcga aaaaaattct
     2761 tatacggact gatcgccgtt ttcgttgcgg gagggattgt aggcctttgg gatatgtacc
     2821 ggtggctgaa ggatttcggg atgaatttag atccgcgtgc accgatcgat cttgagccat
     2881 tcatcccgcc gatcatcggg aaaaacatca tcgcgaactt tacgacaatc agctatttta
     2941 cgtatggtgg ctatatgctg ctgctcgttt tcatacttgt cgtctatccg ctatggaagg
     3001 acaggaaaaa gccatgaaga agatcatcta cttatcgttt ctaagttttt tgttcctaac
     3061 cggccaagca gatgcacaag acgtcggaaa cttgcagcaa ctcattgatg aaacaccgat
     3121 tggcggcgta ttggagctgg aagacgccac gtacgaaggc aacgtcgtcc tgaccaagcc
     3181 aatcacgata aaagggaaaa aaggaacacg catcaaaggc gataagacgg tcaatgtcat
     3241 cgaagtgcgc agcaaggatg tgacgctcga tacgctgtca atcgaaggga gcgggatgag
     3301 ccgtagttcg caggaagagt attctgcaat ccgtgtcatg gcagatgggg cggttatgag
     3361 gaatttgcat attgcggata gctttcatgg gatttatttg aacaagacga aaaacacagt
     3421 gatttcgcat gtgaagattg tcgggcaagg gacggatgct gtcggaaacc aagggaatgg
     3481 cgtccacatc gcccgctcca gcgataatca tatcgacaac tgcgatatcg agaaaacacg
     3541 tgacggaatt ttcgtcgagt actccaataa caacgtcatc atcgacaata cgatgtcgca
     3601 aacccgttac ggtttgcact atatgtattc caataacaat gagttcaaac ggaataattt
     3661 cgtcaacaac gtcggcgggg cggcgatcat gcattcggac catatcatac ttgcggataa
     3721 taaattctca ttcaatcaag ggtcgcgctc tttcggtttg attgtacaga cgagccggga
     3781 tgtccacgtg ttgaataatg aattccattt gaaccagcgt ggtctgtatt tggagcagtc
     3841 gacgagcaat gtcattgaag gaaatgattt tttccaaaat caaatcggtg tggaactctg
     3901 gacatcctcc actgcccatg tgtttgtcaa aaataacttc cggaaaaatc ggatccatgc
     3961 attgacggtc ggcggtgaat cgaataatga gtggttcagg aacggggaag ggaactactg
     4021 gaatgttccg atgcttgact tcaatcggaa tgggatcggc gatgcgccga tggaatatac
     4081 atccgctttg ggagaactgg tagaaacgaa tgagctttcc tatctgttcc tctccagtcc
     4141 ggcgatcacg atttacgaaa aagcgaatga gttgatgacg ttccagaagg tgatggcgta
     4201 tgacaaatat cctttgatga acagtcaaaa atatagaggg catgaatggg cgctgttggc
     4261 agctgtgcta gtgctggcgg gatggctcgt ttatcgaaag aaggcgaaga aaagatgatg
     4321 tacgtatgga aagaattcct tgaacagacg aggggaaaag gattgtggct tggcatcggg
     4381 gtgctcatgc ttgctacgct cttcctcata gcggaagccc gctctttccc ggcggatcta
     4441 gggtttgagg cgatgttgct atcggttttt gatatgaata tgtaccttct gcccctattc
     4501 gcaatgtttc tctcatcttt ctccatattt caggagaagg aattgaagac atctatgatt
     4561 ttattgacga aaagagagtc gacgcgttct cttttattta aaaaatcagt ggcaatccaa
     4621 gtcgtacttc tgtccatttt tctagttgcc tatttcatct tggctctttt catgaaagtg
     4681 tttttggctt ttcatgtcgt cagttttctg catttcctgc tcgtgatcac tgtgtttctt
     4741 cttattttca atcagctggg gattttcctc ggggttgctt gcaatacaaa gatgcagctg
     4801 gtcggggcga atatattaac ttggtttttc gttgtcttcc tgtttgacct gatgttcctc
     4861 tatttcttgc cggctgtgac attcgacaat ctacacttct tctcatgggt gtatttcttg
     4921 gatccgattc atactttgcg gttttattta gagacaagtc taggactctt ctcattaagc
     4981 catatgtcga gaatgatgga gaatttcgtt ttcatggata tgaagtattt ccttgctatc
     5041 aacgttctgc tttggcctgg actgttgttc ggcctatcta ctctcttccg gaatggaggg
     5101 acaatcgatg attaagattc aaaacgtatc gcatgcctat cataagaaaa aggtgttgga
     5161 tgatgtcagc ttgcatatcg gagcgaatga gcgatgtgcc ttagtagggc gcaacggtgc
     5221 aggcaagtcg acgatgatcc atgtcatgct gggtttgctg ccgatccaga aagggtcaat
     5281 cgtgctaggc ggcatcccga ataaaaagca ggaatggaaa cggatcgtct cgtatttgcc
     5341 cgaaaaattc cacctctatc cccatctgac aggggaagag aatatgcggt tctttgcatc
     5401 cctttcgacg aaaaagatcg atgaagagag catagcggaa aaattgaaaa tggtttcatt
     5461 atacgaagcg cgcggcgaac agatcacgaa gtattcaaaa gggatgctgc aacggttggg
     5521 ccttgcagtc atgctgtact acgattcgga catcatcatt ctggatgagc cgacgagtgg
     5581 gctggatccg atgggacggg aagaaatatt gcagatcatt aattcattgg acaataaaac
     5641 aatcctgatg gcatcgcatc acctttccga aatagaaaaa gtgtgtacgc acgtcgcatt
     5701 cctcgaagat ggaaaggtga taaaatatcc ggttgatgaa tttatccgga cgcatctgaa
     5761 ggaggagtcg aaatgaaaag acttgtagta agtttgcttc ttatagtagt gctggcagcc
     5821 gggtgcagtt cagccaagat ggacatttca atcgaaaaga tgcctgaatc caaaacaggc
     5881 acgccatccg aaatgcttat caaagtggcg ggagatggtg ttggcttgga agggttgtcg
     5941 gtcgtcgcct cacttgaaat ggcgaagatg gatcacggtg aacttgaagt gcaattcgaa
     6001 gaaatcggtg atggcctgta cagcgggcaa gtggaattgc cgatgggtgg cgaatggatt
     6061 gctgatgtgg aagtgaaatc cggggaagag actgtgaaag aaattttgac agtcgaagtt
     6121 gaagagagat gatgatcatg aataaaaggt ggatacccat catcattcta ttatcagcgc
     6181 tatggttggg cggttgtgcg gagaaagcag aaacgcccga acaaagcttg gcggaacctg
     6241 tcgaaacgat ggatcgtgga
//
LOCUS       DASULI010000325         5417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1235628,
            whole genome shotgun sequence.
ACCESSION   DASULI010000325 DASULI010000000
VERSION     DASULI010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235628"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFX34_06955"
     CDS             complement(<1..588)
                     /locus_tag="VFX34_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5564682.1"
                     /translation="MQSIRKILFFTTEYLKQLKKKRATLLLLFLFPLILIGLLVGLAA
                     GLIIPEENSPIRVALVDEDRTKESKLISTLLTETANGQSTIEILPASKEEAGRLMKEN
                     EISTSFSLPKNFTDDLYEGETVTIPMIGNPSRPIDSFIAKQLVDSLARYIAAAQANIL
                     VINDYAKGTSMTREERMEMMFEQFIDFTLYTLGKDQ"
     gene            complement(607..2196)
                     /locus_tag="VFX34_06960"
     CDS             complement(607..2196)
                     /locus_tag="VFX34_06960"
                     /inference="COORDINATES: protein motif:HMM:NF042642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6583 family protein"
                     /protein_id="HEX5564683.1"
                     /translation="MENVQEKKVKKKSPALLIAVLTAVLVIGAGAVSAFFLFNKSPKV
                     KYLLAETKTFTQIGDLFEERYKNEVDWMDVQKTKPVETTYDLSAEWNDPNAGYEMAEV
                     QRILNNTTFSMTQVKDPVEKEWEMNFSGKFGSISSDFATLYFTPEKMMASLPFTDKLL
                     FFMDEDYGKMMRETDAFYEGPEKLGLSHLFEEQFSMDGEFQKYVKEEYMEYLYHELPE
                     EAFTSDKEEIEVFGEKMKTEKVAMKLSEEQVKSLLVDLFKKARDDEKLKALLKEQIAF
                     STMTDDISDRKLTELIRDFEDSLDEAIDGVNKLHIPNGLESTIWHKSNSIVKREFAMS
                     IGESQDEIAALKFAGVQLLEKSGQKWDYSITGTDPYMQEDVVVDFTGDLTWKDGKSED
                     TITITAEDVSITYKGKEELDGKERKFKREFAVSDEDFSPKLVWSGKAAHEKDSMKADH
                     EFTIIDPSGAISTTDIYLRLKQEGKIVKKVEMPDESKNLVDVGKMSGDELTDLGDELA
                     QEFEEWVTELMGDLQSELENF"
     gene            complement(2393..3601)
                     /locus_tag="VFX34_06965"
     CDS             complement(2393..3601)
                     /locus_tag="VFX34_06965"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010678418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5564684.1"
                     /translation="MGNKKVVLAYSGGLDTSVAVQWLTDKGYDVVACCLDVGEGKDLH
                     FIQQKALQVGAVNSYVIDAKEEFAQEYALLALQARTFYEDKYPLVSALSRPLISKKLV
                     EVAHKENATAVAHGCTGKGNDQVRFEVAIKALDPSLEVIAPVREWSWSREEEIAYAKE
                     KNIPIPINLDSPYSIDQNLWGRSNECGILEDPWAAPPEEAFGLTASIEDAPDQPDIIE
                     IEFVKGVPVSIDGKQYPLSELIVKLNEVAGKHGVGRIDHVENRLVGIKSREVYECPGA
                     VTLLKAHKELEDITLVKDVAHFKPLVSQKLSELIYNGLWYSPLRNALEAFVKETQQYV
                     NGVVRVKLFKGHAIVEGRKSSNSLYDEKLATYTSDDEFDQSAAVGFIQLWGLPTEVHS
                     SVNKKQKVKN"
     gene            complement(3879..5258)
                     /gene="gdhA"
                     /locus_tag="VFX34_06970"
     CDS             complement(3879..5258)
                     /gene="gdhA"
                     /locus_tag="VFX34_06970"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX5564685.1"
                     /translation="MTNMGNNRDNRSRANDYVHEVYELVKKRNPDEKEFLQATREIFD
                     SLRPVFAKHPEYIRNGILERITEPERIISFRVAWEDDKGKVHVNRGYRVQFNSDLGPY
                     KGGIRFHPSVNNSIMKFLAFEQIFKNALTGQPIGAGKGGSDFNPKGKSEREIMRFTQS
                     FMTELSKHIGPDIDVPAGDIGVGKREIGYMFGQYNRLKGGYEAGVLTGKDPNHGGSLG
                     RKEATGYGTVYFVEEMLNENGLSFKDSTVVVSGSGNVSTYAMEKAQQLGAKVVACSDS
                     GGYIYDKNGIDLETVKRLKEVENKRIWEYTKEHPDAEYHDDCTAIWSIPCDIALPCAT
                     QNEIDEIAAHMLIANGLKALGEGANMPCNIEAIDIFLENNILFAPAKAANAGGVAVSA
                     MEMSQNSMRLAWTEEEVDMRLKTVMENIYSSCKEAADRFGKPGNLVMGANIAGFLKVA
                     DAMVAHGIR"
BASE COUNT         1295 a         1370 c         1082 g         1670 t
ORIGIN      
        1 ctgatctttg cctaatgtat agagcgtgaa atcgatgaat tgctcaaaca tcatctccat
       61 ccgctcttcg cgtgtcatcg aagttccttt tgcgtaatca ttaatcacta atatattcgc
      121 ctgggctgca gcaatgtatc tggccaaact atctacgagc tgcttcgcaa tgaaactatc
      181 gatcggcctg gatgggttgc caatcatcgg gattgtcacc gtctcccctt cgtacagatc
      241 atccgtgaaa tttttcggca atgaaaaaga ggtgctaatt tcattctctt tcattaagcg
      301 ccccgcttcc tccttggatg ccggaaggat ttctatggtg ctttgcccgt ttgccgtttc
      361 cgtcagcaac gtgctgatca gcttcgattc ctttgtccgg tcctcgtcaa ccaatgcgac
      421 acgaattggt gagttctctt cggggatgat gagcccagcc gcgagaccga ccaataagcc
      481 aatcaaaatt agcgggaata gaaaaagaag aagaagggtc gcccgcttct tcttcagctg
      541 tttcaagtat tcagtcgtaa aaaacaatat ttttcgtatc gattgcaaac agcccacttc
      601 caatcatcag aagttttcaa gctcactttg gaggtcgccc atcagttcag ttacccactc
      661 ctcgaactct tgagccaatt catctccaag gtcggtcagt tcatcgccgc tcatcttgcc
      721 gacatcgaca agatttttgg attcatccgg catttccact ttcttcacga ttttcccttc
      781 ctgtttcaga cgaaggtaaa tgtcggttgt tgaaatggca cctgacggat cgatgatcgt
      841 gaattcgtga tccgctttca tcgaatcttt ttcatgcgca gctttaccgc tccacacgag
      901 tttaggggag aaatcttcgt cggatacggc aaattcccgc ttgaatttgc gttccttccc
      961 atccagttct tctttccctt tatacgtaat ggatacatct tctgcagtaa tcgtgattgt
     1021 atcctccgac tttccgtctt tccacgtcaa atcgcctgtg aaatcgacga caacgtcttc
     1081 ctgcatataa gggtctgtgc cggtgattga ataatcccat ttctgtcctg atttttccaa
     1141 caactggaca ccagcaaatt tcaaagcagc aatttcatct tgtgattcac cgattgacat
     1201 ggcgaattcc cgttttacga tactattgga tttatgccaa atcgtcgatt cgagtccatt
     1261 cggaatatgc aatttattga ctccatcaat tgcttcgtcc aatgaatctt caaaatcacg
     1321 gatcaactcg gtaagcttcc ggtcggaaat atcatctgtc atcgtcgaaa aggcgatttg
     1381 ttctttcaag agcgccttca atttttcatc atcccgcgct ttcttgaata gatcaactaa
     1441 aagtgacttc acttgttctt ccgaaagctt cattgccact ttttccgttt tcatcttttc
     1501 gccgaatact tcaatttctt ctttatcact tgtgaacgct tcttcaggca actcatgata
     1561 aaggtattcc atatactcct ctttcacgta tttctggaat tccccgtcca tggaaaactg
     1621 ctcttcaaat agatgggaca ggccgagctt ctccgggcct tcgtaaaaag catccgtttc
     1681 cctcatcatt ttgccgtagt cctcatccat gaaaaacaga agcttatccg tgaacggcag
     1741 tgacgccatc attttttcag gtgtgaaata caatgtcgcg aaatccgatg aaatagagcc
     1801 gaatttcccg ctgaaattca tttcccattc cttttcaact ggatccttca cttgtgtcat
     1861 cgaaaatgtc gtgttgttca ggatcctttg cacttcggcc atctcataac ccgcattcgg
     1921 atcattccat tccgccgaaa ggtcataggt cgtttcaacc ggcttcgtct tctggacatc
     1981 catccagtca acttcgtttt tatagcgttc ctcgaaaagg tctccgattt gagtgaacgt
     2041 cttcgtttca gccaataagt atttcacttt tggtgatttg ttaaatagaa agaatgcgga
     2101 aaccgctccg gcaccgatga ccagcacagc tgtgagcacg gcaatcagta atgcgggcga
     2161 cttctttttc actttcttct cttgaacatt ctccactgaa tttcctccct gactagcttg
     2221 atagattagc aatcctacgc atattagaaa ttctactata aaaaaaggat aattgttcct
     2281 tatttcacaa attgattatt tatagctagt tatcaatctt agggattaag ggctattcgt
     2341 gcaggttgac aggaaaaagc ctcctgcatt tttctgcaga aggcaaggtc gatcagtttt
     2401 tcactttttg ctttttattc accgagctat ggacttccgt cggcagaccc catagttgga
     2461 tgaagccgac agctgcagat tggtcgaatt cgtcatcaga cgtgtaggtc gccagtttct
     2521 catcatagag ggagttggac gattttcttc cttccacaat tgcatgccct ttgaacaatt
     2581 tgacacggac aacaccgttg acatattgct gcgtctcttt cacgaaagct tccaacgcgt
     2641 ttcggagcgg cgagtaccat aagccgttat agatcaattc actaagcttc tgggaaacga
     2701 gcggcttgaa gtgggcgacg tctttgacga gtgtaatgtc ttccaattcc ttatgcgcct
     2761 tcaataatgt aacggctcct ggacattcat acacttcacg tgacttgatg ccgacgagtc
     2821 ggttttcaac atggtcaatt ctgcctactc catgctttcc ggcaacttcg ttcaatttaa
     2881 cgatcagttc actcaatggg tattgttttc cgtcaatgct gacagggacc ccttttacaa
     2941 attcgatttc aatgatatcc ggctgatccg gcgcatcttc aatcgaagcc gttaaaccga
     3001 atgcctcttc aggcggagca gcccatggat cttcaaggat gccgcattca ttgcttcggc
     3061 cccatagatt ttggtcgatg gaataagggc tgtcgagatt gatcggaatc gggatattct
     3121 tctctttcgc ataagcgatc tcctcttcac gcgaccagct ccattcacgc acaggcgcaa
     3181 tcacctctag cgacgggtcg agcgctttga tggccacttc aaaacggact tggtcattcc
     3241 cttttccggt gcagccatgt gccacagccg tcgcattttc cttgtgtgcc acttcgacaa
     3301 gctttttgga aatcaaagga cgagataaag cggaaacgag aggatatttg tcctcgtaga
     3361 aagttcgagc ctgcaatgca agcaaagcat actcctgcgc aaattcttct ttcgcgtcga
     3421 ttacatagct gttcaccgca ccgacttgca acgctttttg ctgaatgaag tgcaagtctt
     3481 tcccttcccc gacatccaag caacatgcga cgacgtcata ccctttatct gtcaaccatt
     3541 gcaccgccac cgaagtatca agaccacctg aatacgccaa tacaactttt ttatttccca
     3601 ttcatttcat cctcctctat acatctcact gcatctttat tcatctttgt gtatttttat
     3661 aataccacat atatttttaa tatcaataga tttgcatgaa atattattgc tgtggatttt
     3721 atgagcggat ttggggattt atgcgtgatt gcggttgttt atgcgtatgt atgcggtttt
     3781 atgcgcagtt acggcgattt atgcgccgtt gtgaagattt atggatatga aataaaaaac
     3841 taccttacta actggcactg taaggtagtt tcggatattt atctgatgcc gtgggcaacc
     3901 attgcatcgg cgactttcag gaatcctgca atattggccc ccatgacaag attgccgggt
     3961 ttgccgaaac ggtccgctgc ttccttacag cttgaataga tgttttccat gaccgttttc
     4021 aggcgcatgt ccacttcctc ttccgtccat gcaaggcgca tgctattttg tgacatttcc
     4081 atcgcagata cagccactcc gcctgcgttt gccgcttttg caggagcaaa caggatgtta
     4141 ttttcgagga aaatatcgat cgcttcgata ttgcatggca tattggcgcc ttccccgagc
     4201 gctttcaatc cgtttgcaat gagcatatgg gccgcgattt catcgatttc attctgtgta
     4261 gcgcaaggaa gtgcgatatc gcaaggaatg gaccagattg ccgtgcaatc gtcatggtat
     4321 tcggcatccg gatgctcctt cgtatattcc caaatccgct tattctccac ttctttcaac
     4381 cgtttcaccg tttcaagatc gattccattc ttgtcataga tatatccgcc ggaatcgctg
     4441 catgcaacga cctttgctcc aagctgctgg gctttttcca ttgcgtacgt cgatacattt
     4501 ccggaccccg atacaacgac cgtactatct ttgaaggaaa gtccgttttc attcaacatc
     4561 tcctcgacga aatagacggt gccataaccg gtagcttcct tccgacctaa cgaaccgcca
     4621 tgattcggat cttttcccgt caggacgccc gcctcgtagc cgcccttcaa ccggttgtat
     4681 tggccgaaca tatagccgat ctcccgtttg ccgacaccga tatcacccgc aggcacgtca
     4741 atatcaggac ctatatgctt gcttagctcg gtcatgaagc tttgtgtaaa gcgcatgatt
     4801 tccctttcgg acttcccttt cggattgaaa tcggagccgc ccttccccgc accgattggc
     4861 tgccccgtca atgcattttt gaatatctgt tcgaatgcca ggaacttcat gatgctatta
     4921 ttcacggaag gatggaagcg tatgccgcct ttatatggac cgagatcgct attgaattgc
     4981 acgcggtatc cccgattgac atgcactttt cctttgtcgt cttcccaggc gacacggaaa
     5041 gagatgatgc gctcgggctc agttatgcgc tctagaattc cgtttcgtat atattcagga
     5101 tgcttggcaa aaaccgggcg aagcgaatcg aatatttctc tcgtcgcttg tagaaattcc
     5161 ttttcatccg gatttctttt cttcaccaat tcatatactt catgaacgta gtcattcgcc
     5221 cggctccggt tatctcggtt gttccccata tttgtcatct gcctcatcct ttcttgaaaa
     5281 tagcgaagaa aactgtggaa gctatctaaa gttttagtga aaattaatat ttcgaaatcg
     5341 tttcttcgtt ccatttagta ttactgaaaa tatgaatata tacaataact ttttcaattt
     5401 gcagttattt acttgtt
//
LOCUS       DASULI010000326         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1943935,
            whole genome shotgun sequence.
ACCESSION   DASULI010000326 DASULI010000000
VERSION     DASULI010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943935"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /gene="paaA"
                     /locus_tag="VFX34_06975"
     CDS             <1..854
                     /gene="paaA"
                     /locus_tag="VFX34_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX5564686.1"
                     /translation="EYRLTLIKLISMHGISEIMGALPEKEWVPKAPSLQRKLGIMAKV
                     QDEMGHGQLLLRVAEDLLAPYGKNRGDLMQDLFNGDLKFHNVFHMETKTWADAGIIGW
                     LVDGAAIITQTDMLGASYGPYARALQRICAEEVFHAQHGESIIMALAEGTDDQKAMIQ
                     EALDRWWEALLMFFGPASKETTGSSKQDVTIKYKIRTKTNEDFRQIFFDKYIPRILSL
                     GLTIPDPTFHYDQESGLWSYKQPDWEEFKRIIKNKGPRSQARLNLRRSSYENNAWVRD
                     ALSAAIN"
     gene            874..1221
                     /gene="paaB"
                     /locus_tag="VFX34_06980"
     CDS             874..1221
                     /gene="paaB"
                     /locus_tag="VFX34_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX5564687.1"
                     /translation="MAEEKLFYQEYEVFSKRTPSSAFQHQFSLLAPNEEMALVLAQEN
                     FMRREPVTDIWIVNRKHIRKMDAAEKLTLRRLDNKDYRLTKGYGYLKKKWRHYEQEIL
                     DEKEILSWGGKRK"
     gene            1218..2030
                     /gene="paaC"
                     /locus_tag="VFX34_06985"
     CDS             1218..2030
                     /gene="paaC"
                     /locus_tag="VFX34_06985"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX5564688.1"
                     /translation="MSEIENVLTAEHKEAITSLLFQLADDDFLYAYRGSEWLGLAPHI
                     EEDVASSSISQDSMGHGAMYYKLLEELGAGNADDLAHLRPAQDRRNSILTERVNGEGY
                     YMETPNYDWAYQVVRSYFYTQAKKVKVDSLCQSSYRPLAEAAVKVKMELYYHRMHWET
                     WFKQLLSSTDVAKKKMNDAILRVMNDFGDMFSYGNQKLAIENYKLIDSEEKLKENWIA
                     SLEPVFAALQMVVPAIPENPANNGRNGEHTVDLDEALLTLSEVYKSDPVASW"
     gene            2054..>2549
                     /gene="paaD"
                     /locus_tag="VFX34_06990"
     CDS             2054..>2549
                     /gene="paaD"
                     /locus_tag="VFX34_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX5564689.1"
                     /translation="MAIYESVSTDCIFEALTNVKDPEIDSVSIIDLGMVEEVTTLGNN
                     VKVVLLPTFLGCPALEMIKKNTIDAIKQLPEVEEVEVDFVFHPPWTSDRITEKGHVNL
                     RAFGIAPPPRHVEEDGSWHVDCAYCGSTYVTMENIFGPTACRSILYCKSCKNVFEAMK
                     PVSTL"
BASE COUNT          780 a          496 c          651 g          622 t
ORIGIN      
        1 aagaatacag actaacattg attaaactga tttccatgca cgggatcagc gagataatgg
       61 gagcccttcc cgagaaagag tgggtaccga aagccccgtc tcttcagaga aaacttggca
      121 ttatggcaaa agtgcaggac gaaatggggc atggtcagct gctgcttcgg gttgcggagg
      181 acttactagc gccatatgga aaaaatcgtg gggatttaat gcaggattta ttcaatgggg
      241 atctgaaatt tcataatgtg ttccatatgg aaacgaaaac atgggcggat gcaggaatca
      301 tcggttggct cgttgatggg gcagccatca ttacacagac ggatatgctt ggggcttcct
      361 atggacctta tgcaagggca ctgcagcgca tttgtgccga agaagtattt catgcacagc
      421 atggcgagtc aatcatcatg gcgttggcag aaggaacgga tgatcaaaag gcgatgattc
      481 aagaagccct tgatcgctgg tgggaggcat tgctcatgtt ctttggaccg gcaagcaaag
      541 agacgacggg ttcatcgaag caagacgtaa cgattaaata caaaatccgt acgaagacaa
      601 atgaagactt taggcagatc ttttttgaca aatacattcc gcgtatttta tcgcttggcc
      661 taaccattcc ggatccgaca ttccattacg atcaagagag tggattatgg tcgtacaaac
      721 aaccggactg ggaagagttt aagaggatta tcaaaaataa aggacctcgc tcacaagcgc
      781 gtctgaacct gcgccgatct tcttatgaaa ataatgcttg ggtacgcgat gcactgagtg
      841 ctgcaatcaa ttaaagaaag gagtcagaag gaaatggccg aagagaaact gttttatcag
      901 gaatatgaag tttttagcaa acggacgccg agttcggcat tccagcacca attcagctta
      961 ctggctccca atgaagaaat ggcactcgtt ttggcacagg aaaatttcat gcgccgcgaa
     1021 ccggtcactg acatttggat cgtgaatcga aagcatattc ggaaaatgga tgcagctgag
     1081 aaattgacac ttcgcagact ggacaataaa gattatcgtt tgacgaaagg ctatggctac
     1141 ttaaagaaaa agtggcgtca ctatgaacag gagatcttgg atgaaaaaga aatcttgtca
     1201 tggggaggaa aacgcaaatg agcgaaatcg aaaacgtgtt gacggcggaa cacaaggaag
     1261 cgataactag cttgctattc caattagcgg acgatgattt cttgtatgcc taccgtggat
     1321 ccgagtggct cggactcgct cctcatattg aggaagacgt cgcttcttca tcaatcagcc
     1381 aagacagtat ggggcatgga gctatgtact acaaacttct ggaagagctg ggggcgggaa
     1441 atgcagatga tctggcccat ttgcgcccgg cacaagatag aaggaacagc atactgacag
     1501 aacgggtgaa cggtgaaggt tattatatgg aaacacccaa ttatgattgg gcctatcaag
     1561 ttgtcagaag ttatttttac acgcaagcaa aaaaagttaa agtcgattct ttatgccaaa
     1621 gttcgtatcg cccccttgct gaagctgcag tgaaagtgaa aatggagctt tattatcaca
     1681 ggatgcattg ggaaacttgg ttcaagcaat tattgagctc taccgatgtt gcaaaaaaga
     1741 aaatgaatga cgccattctt cgggttatga atgactttgg ggatatgttc tcctatggga
     1801 atcaaaaact agcaattgaa aactataagc tgatcgacag tgaggaaaaa ttgaaagaaa
     1861 actggatagc cagccttgaa ccagtttttg ctgctcttca aatggtcgta ccggcgattc
     1921 cggaaaatcc cgcgaataac ggaaggaatg gcgagcatac agtagacctg gatgaagctc
     1981 tgcttacact ttctgaagtc tacaagtcag atccggtggc ttcctggtaa tgaaagagga
     2041 aagtggtgaa ttcatggcca tttatgaaag cgtttccact gattgcatct ttgaagcact
     2101 tacgaatgtg aaagatcctg aaattgattc ggtcagcatt atcgaccttg gcatggttga
     2161 agaagtcacg accttgggga ataacgtgaa agtggtttta ctgcctacat ttttaggatg
     2221 tccggcatta gagatgatca aaaagaatac aattgatgca atcaaacaac tgcccgaagt
     2281 ggaagaggtc gaagttgatt tcgtctttca tccgccgtgg acgtcagatc gcattactga
     2341 aaaagggcat gtgaatctgc gggcgttcgg aatagcaccg cccccgcgac atgttgaaga
     2401 ggacggctcc tggcatgtag attgcgccta ttgcggatcc acctatgtga caatggagaa
     2461 cattttcggg ccgactgctt gcagaagcat tttgtattgt aaaagctgta aaaatgtatt
     2521 tgaggcaatg aaacctgttt caacactac
//
LOCUS       DASULI010000327         4618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1177247,
            whole genome shotgun sequence.
ACCESSION   DASULI010000327 DASULI010000000
VERSION     DASULI010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177247"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFX34_06995"
     CDS             complement(<1..712)
                     /locus_tag="VFX34_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5564690.1"
                     /translation="MKNTFSRFFGSGEKEPDLQNIDNTPQEKVDQVKIDLIKPNKYQP
                     RTIFSEEKIEELARTIHTHGVIQPIVIRNSGDGYEIIAGERRFRAMKKLGWEEVPAII
                     RNLDDKETASIALIENLQREELTAIEEAYAYEKLLELHGLTQEALAQRLGKGQSTVAN
                     KLRLLKLPDEIKNGILTKEISERHARALISVKDPELQQKLFQEAIEEQLNVKQLEERI
                     QQALNPIEEEKEKKRVVKR"
     gene            complement(958..1674)
                     /gene="rsmG"
                     /locus_tag="VFX34_07000"
     CDS             complement(958..1674)
                     /gene="rsmG"
                     /locus_tag="VFX34_07000"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX5564691.1"
                     /translation="MNEEQFIEALKEQGIDLSNQQLQQFDKYFHMLVEWNEKMNLTAI
                     TDAPSVYLKHFYDSITAAFHTDLNGTKSICDVGAGAGFPSIPLKICFPDLSVTIVDSL
                     NKRITFLTELAKELEIDNVRFVHARAEDFGQDPKYREQFDIVTARAVARLSVLSELCL
                     PLVKEGGIFISMKGAAAEEELLDAKKAINVLGAELDGKFSFSLPMEESERNIFILNKV
                     KKTPKKYPRKPGIPNKTPIK"
     gene            complement(1736..3625)
                     /gene="mnmG"
                     /locus_tag="VFX34_07005"
     CDS             complement(1736..3625)
                     /gene="mnmG"
                     /locus_tag="VFX34_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX5564692.1"
                     /translation="MPQFEAGTFDCIVIGAGHAGVEAALASAKMGASTLVLTMNLDMI
                     AFMPCNPSLGGPAKGIVVREIDALGGAMGKVIDKTHIQMRMLNTGKGPAVRALRAQAD
                     KVLYQQEMKRVLEEQENLTLHQGVVEELIVEDNEVKGLVTQIGAIYRAKTVIITTGTF
                     LRGEVIIGDLKYSSGPNNQMPAIKLAENLQELGLETVRFKTGTPPRINGKTIDYSKTE
                     IQPGDDVPRAFSYETTQFIMDQLPCWLTYTTPRTHEIINENLHLSPMYSGMIKGKGPS
                     YCPSIEDKIVRFADKSRHQIFLEPEGRNTKEYYVQGLSTSLPEHVQRRLIESVPGLEK
                     AEMMRAGYAIEYDSIIPTQLWPTLETKKIKNLYTAGQINGTSGYEEAAAQGIMAGINA
                     ASKVLGKEEVILGRSDAYIGVLIDDLVTKGTSEPYRLLTSRAEYRLLLRHDNADLRLT
                     EIGYKLGLISEERHEKFLLKKQQIEEEIERLRKVTIKPDEAVQEIIRESGGTELREPM
                     KAADLLKRPEMKYDQIITVVPAEEAKSDEVAEQVEIFIKYEGYIEKSMQQVERMKKME
                     NKRIPENIDYHAISGIAKEAKANLSAVRPLSIAQASRISGVNPADISILLVYIEQGKI
                     AKITG"
     gene            complement(3672..>4618)
                     /gene="mnmE"
                     /locus_tag="VFX34_07010"
     CDS             complement(3672..>4618)
                     /gene="mnmE"
                     /locus_tag="VFX34_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX5564693.1"
                     /translation="NVALNQMEGKLSKLIGELRQALLETLAQVEVNIDYPEYDDVEEV
                     TIPLMIEKGTWVKEEIEKLLRTSSQGKILREGLSTVIVGRPNVGKSSLLNSLVQENKA
                     IVTDIAGTTRDIIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVERSRQVLKEADLIL
                     LVLNSSEELTEEDMRLFEATEGMDMIVVINKTDLPQKIDMQQVIELAGRGAIVTTSIL
                     KEEGIDELEEAIAKLFFEGTLEAGDLTYVSNARHIALLHKAHKTIQDALSAAEGGVPV
                     DMIQIDVTRTWELLGEIVGDTVQDSLINQLFSQFCLGK"
BASE COUNT         1048 a         1115 c          957 g         1498 t
ORIGIN      
        1 tccttttaac gactctcttc ttttcctttt cttcctcaat tgggttgaga gcttgctgaa
       61 tccgctcttc caattgcttt acattgagtt gttcttcgat tgcctcttgg aatagctttt
      121 gttgcaattc aggatccttc acggaaatta aagctctcgc gtgtcgttcg gatatttcct
      181 ttgtaaggat accatttttg atttcatccg gaagtttcaa taaacgcagc ttattggcaa
      241 cggttgattg tcccttccct agtctttgcg ctaaagcttc ctgcgtcaat ccgtgcaatt
      301 ccagcaattt ttcataagca tacgcttctt cgattgccgt caattcttca cgctgcaagt
      361 tttcaatcaa tgcaatggaa gctgtttctt tatcatcaag atttcggata atggccggga
      421 cttcttccca acctagtttc ttcattgcac gaaatcttcg ttcacctgca atgatttcat
      481 atccatcgcc gctgtttcga atgacgattg gttgaatcac tccatgtgta tggatcgttc
      541 tagcaagctc ttcaattttc tcttcggaaa aaatggttcg aggttgatat ttattcggtt
      601 ttatcaaatc aatttttact tgatcgactt tctcttgtgg agtgttatct atgttctgta
      661 aatcaggttc tttttctcca ctaccaaaaa accgtgaaaa cgtgtttttc atcgccggca
      721 cccaccttta caaaactgct cttcgttaat agctatttcg tcatatctat tatatatcct
      781 ctttattact tattaaagag tagaattcta taagactaca attctatagc ttatgttact
      841 agcctctgta ataaaaaggt agtataaaga gaggcgcgat gtttcatcga gcgcctgccc
      901 ttcatcttca ataaatcaag aattcatgag aagtagataa tgtttcacgt gaaacattta
      961 tttgattggc gttttatttg ggatgcctgg ctttcgagga tatttcttag gtgtcttctt
     1021 cactttattc aagatgaaaa tgtttctttc actttcctcc attggcaagc tgaaagaaaa
     1081 tttcccgtcg agttccgctc ccaatacgtt aatcgctttt tttgcatcta gcaattcttc
     1141 ttcggcagct gcacctttca tagaaatgaa aatgccccct tcttttacaa gtggtagaca
     1201 tagctccgaa agcaccgata aacgggcaac tgcacgggca gtgacaatgt cgaattgctc
     1261 acgatatttt gggtcttgtc cgaaatcttc ggctcttgca tggacaaatc ttacgttatc
     1321 gatttcaagt tccttagcaa gttcagttaa aaaagtgatt ctcttgttta acgaatctac
     1381 aatcgttacg gataaatcag gaaaacagat ttttaaagga atgcttggaa atcctgcccc
     1441 cgcacctaca tcacaaatgg atttagtacc gtttaaatct gtatggaacg cggctgtaat
     1501 ggagtcataa aaatgtttca gatagacgga tggtgcatct gttattgcag tcaaattcat
     1561 tttttcattc cactcgacta acatatggaa atatttgtca aattgttgaa gctgttgatt
     1621 tgaaaggtca atcccctgtt ccttcaacgc ctctatgaat tgttcttcgt tcaattggat
     1681 cctccttctt aaaaaaagac tgatgcggac ttatccccac atcagtctca tgatgtcacc
     1741 ctgtaatttt cgcgattttt ccttgttcga tatagaccag caggatggag atgtctgcag
     1801 ggttcacacc ggaaatcctt gatgcttgcg caatggataa cggtctaact gcggacagat
     1861 ttgctttcgc ttctttggca atccctgaaa ttgcatggta atcaatgttc tcaggtattc
     1921 ttttgttctc catcttcttc atgcgttcca cttgctgcat cgatttttcg atatagcctt
     1981 catacttgat gaagatttca acctgctccg ccacttcatc ggattttgct tcttcagctg
     2041 gcacaacagt gatgatttgg tcgtacttca tttcaggacg ttttaataga tccgctgctt
     2101 tcatcggttc acggagttct gtaccacctg attcacggat gatttcctgg acggcttcat
     2161 ccggtttgat cgtcactttt cgaagtcgtt cgatctcctc ttcaatttgc tgctttttga
     2221 ggaggaattt ctcatgacgc tcttcactga tcagcccgag cttgtatccg atttccgtta
     2281 ggcgcagatc cgcattgtca tgacgcagca agagacggta ttccgccctt gatgtaagaa
     2341 gacgataagg ctcgctcgtt ccttttgtta cgaggtcatc gatcagaaca ccgatatagg
     2401 catccgatct gccaaggatg acttcttctt tgccaagcac tttgcttgct gcattgatgc
     2461 ccgccatgat cccttgggct gccgcctctt catagccgga tgttccgttg atttgcccgg
     2521 ctgtataaag gtttttgatc tttttcgtct cgagtgtcgg ccaaagctgc gttggtatga
     2581 tggaatcata ttcaattgca tatccagcac gcatcatttc cgccttttcc agccccggaa
     2641 cgctttcgat cagtctgcgt tgaacgtgtt ccggcaaact tgttgaaagg ccttggacgt
     2701 aatactcttt cgtattccgt ccttcgggct ccaagaaaat ctgatggcgc gatttatccg
     2761 cgaaacggac aatcttgtcc tcgattgaag ggcaatagct cggccccttc cctttgatca
     2821 tccctgaata catcggcgat agatgaaggt tttcattgat gatttcatgt gttcgcggtg
     2881 ttgtgtaggt taaccagcaa ggaagttggt ccatgatgaa ttgcgtcgtt tcgtaactga
     2941 acgcacgagg tacgtcatca cctggttgaa tttccgtttt gctgtaatcg atcgtcttgc
     3001 cgttgattcg cggaggtgta ccggttttga aacggaccgt ttccagaccg agttcctgca
     3061 gattctcagc aagtttgatt gctggcattt ggttgttcgg accgcttgaa tatttcaggt
     3121 cgccgataat gacttccccg cgaaggaacg tcccagtcgt gatgatgact gttttcgcac
     3181 ggtaaatggc accgatttgc gtaacaagcc ctttcacttc attatcctcg acgatcaatt
     3241 cttcaacaac tccttgatgg agcgtcaaat tttcctgttc ttcaaggacg cgtttcattt
     3301 cctgttggta aaggacttta tccgcttgtg cccgaagcgc acggacggca ggccctttcc
     3361 ctgtgttcaa catcctcatt tggatgtgcg ttttatcgat cactttcccc attgctccgc
     3421 caagcgcgtc aatttcacgg acgacaatcc cttttgcggg acctccgagt gacggattgc
     3481 atggcatgaa ggcgatcata tcgaggttca ttgttagcac caatgtcgat gcacccatct
     3541 ttgcagatgc caatgcggct tcgacgccgg catggcctgc tccgatgact atacaatcga
     3601 acgtgcctgc ttcaaattgt ggcatgttcg ttcatccttc ctactatata aattttccgt
     3661 ggcaattgaa tttattttcc taagcagaac tgagagaaca gctggttgat gaggctatct
     3721 tggacggtat cgccgacaat ttcgcctaga agctcccacg tgcgcgtgac atcgatttga
     3781 atcatatcga caggcactcc gccctccgct gctgataacg catcctggat ggttttatgc
     3841 gccttgtgga gcaatgcgat atgtcttgca ttggaaacgt acgtcagatc gcccgcctcg
     3901 agcgtccctt cgaagaatag cttggcgatc gcttcttcca attcgtcaat gccttcttcc
     3961 ttcaggatgg atgtcgtgac gattgctcct ctgcctgcca attcgatcac ttgctgcata
     4021 tcgattttct gcggcaagtc ggttttgttg atgacgacga tcatgtccat tccttcggtc
     4081 gcttcgaaaa ggcgcatatc ctcttccgtc agctcctcgg aactgttcag gacaagcaag
     4141 atgagatctg cttccttaag gacttgacgg gatctttcca ccccgatcct ttcgacgatg
     4201 tcttctgttt cccggatccc cgcggtgtcg acgagacgca aaggtactcc cctgacattt
     4261 acatattcct cgataatatc acgggtcgtt cctgcaatat cggtgacaat cgctttattt
     4321 tcctgaacga ggctgttcag caaggaagat tttccgacgt tcggccttcc gacgataaca
     4381 gtcgataagc cttcacgaag gattttccct tgggatgaag tgcgcagaag cttctcgatt
     4441 tcttctttca cccatgtccc tttttcgatc atgagtggga tggtcacttc ttcaacatcg
     4501 tcgtattccg gatagtcgat attcacttcg acttgcgcca atgtttcaag gagcgcttgc
     4561 cgtagttcac caatcagttt ggaaagtttc ccttccatct gattcaaagc aacattca
//
LOCUS       DASULI010000328         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_654616, whole
            genome shotgun sequence.
ACCESSION   DASULI010000328 DASULI010000000
VERSION     DASULI010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654616"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="glyA"
                     /locus_tag="VFX34_07015"
     CDS             complement(<1..698)
                     /gene="glyA"
                     /locus_tag="VFX34_07015"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5564694.1"
                     /translation="MDLSNVKREDTAVYEAIMAEKNRQNANIELIASENFVTEAVMEA
                     QGSYLTNKDAEGYPGKRYYGGCEHVDVVENIARDRVKEIFGAGYANVQAHSGAQANMA
                     VYFTILEPGDTVLGMNLSHGGHLTHGSPVNFSGKLYNFVEYGVSKEDERIDYEDVRQK
                     ALEHKPKLIVAGASAYPREIDFAKFREIADEVGAYLMVDMAHIAGLIAVGEHPNPVPH
                     AHFVTSTTHKTLRGP"
     gene            complement(755..1318)
                     /locus_tag="VFX34_07020"
     CDS             complement(755..1318)
                     /locus_tag="VFX34_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEX5564695.1"
                     /translation="MDDVLNLWKLQLEQLLSEMAEQTTFTPGTFFVVGCSTSEIAGKR
                     IGTGGALEVAEALFGPLQAFAKKHNLSLAFQGCEHINRALTIERKAADKANLDTVSVI
                     PAVHAGGSMSTYAFEHLEDAVVVESIRANAGIDIGQTLIGMHLKSVAVPLRTSIDKIG
                     DAVVTAATTRPKLIGGERAIYTRPLED"
     gene            complement(1336..1773)
                     /gene="rpiB"
                     /locus_tag="VFX34_07025"
     CDS             complement(1336..1773)
                     /gene="rpiB"
                     /locus_tag="VFX34_07025"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX5564696.1"
                     /translation="MKIAISSDHGGNRLRHEIMDLLSELGLDYEDFGPDSDESVDYPD
                     FAKPVADGVASGKFDRGILICGTGIGMSIAANKVKGIRCALVHDVFSAKATRGHNDSN
                     ILAMGERVIGPGLAREVATAWLDTSFEGGRHERRVAKLSELEN"
     gene            complement(1881..>2521)
                     /locus_tag="VFX34_07030"
     CDS             complement(1881..>2521)
                     /locus_tag="VFX34_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5564697.1"
                     /translation="KGIRDMSNQSEQSLGTIRELDQNAQKIGEIVQLVGSIAGQTNLL
                     ALNASIEAARAGEHGKGFAVVAEEVRVLADESAKAVHSIDELVKMIQSDVSKVVKEME
                     KQVSTAANEAERADATSGNVEAMAEKVNGMADSVVKISELVEHQLSNIETTAMQSQEV
                     AAIAEETSAGAEEVRAATEEQVKSIEQTDVMASRLKQQSEDLYKVISQFDRS"
BASE COUNT          485 a          739 c          627 g          670 t
ORIGIN      
        1 ggaccgcgca atgttttatg cgtcgtcgat gtgacgaagt gtgcgtgcgg caccgggttc
       61 ggatgctcgc caacagctat gagtccggcg atatgcgcca tgtcgaccat caagtatgca
      121 ccgacttcat ccgcgatttc acggaattta gcgaagtcga tttcgcgcgg atatgcgctt
      181 gcacccgcaa caatcaattt cggcttgtgc tcaagcgctt tttgacggac gtcttcgtaa
      241 tcgatacgct cgtcctcttt gctgacgccg tactcgacga aattgtacag cttgcccgag
      301 aagttcaccg ggctgccgtg cgtcaagtgt ccgccgtggg aaaggttcat gccgaggacc
      361 gtgtcgcccg gctcaagaat tgtgaaatag actgccatgt tcgcttgcgc gccggagtgg
      421 gcttgcacgt ttgcatagcc tgcgccgaag atttctttca cacggtcgcg tgcgatgttt
      481 tccacgacgt cgacgtgctc gcagccgccg tagtaacgct tgcctggata gccttcagca
      541 tctttattcg tcaagtagga gccttgcgct tccatgaccg cttccgtcac gaagttttcg
      601 gatgcaatca attcaatgtt cgcgttctgg cggttctttt ctgccattat cgcttcgtac
      661 acagctgtat cttcacgttt cacattgctt aaatccatga ttccatttcc ccttttctca
      721 tgtagtctat acgcacttct aattggaatg aactttagtc ttccaacggg cgtgtgtaga
      781 tcgcccgctc gccgccgatc aatttcggac gggttgtcgc cgcagttacg accgcatcgc
      841 cgatcttgtc gatggacgtg cggagcggga cagctaccga cttcagatgc atgccgatca
      901 gtgtctggcc gatatcgatt cccgcattcg cgcggatgga ttcgacgacg accgcgtctt
      961 ccaaatgctc gaacgcatac gtggacatcg atccgcccgc atgcaccgcc ggaatgacgg
     1021 atacggtgtc gagattcgct ttgtccgcag ctttacgctc gatcgtcaag gcgcggttaa
     1081 tatgctcgca gccttggaat gccaacgata ggttatgctt cttggcgaaa gcctgaagcg
     1141 gaccgaataa ggcttcggcg acttcaagtg caccgcctgt cccgatccgt ttgcccgcga
     1201 tttccgaagt cgagcagccg acgacgaaaa atgtgccagg cgtaaaggtt gtctgctccg
     1261 ccatttccga cagcagctgt tcaagctgaa gcttccataa attcaataca tcatccattg
     1321 ccttgctcac ctcacttaat tttcgagctc cgacaatttg gcgacgcggc gctcgtggcg
     1381 gccgccttcg aatgaagtgt ccagccatgc tgtcgccact tcccttgcaa ggccaggtcc
     1441 gatgacgcgc tcgcccatgg caaggatatt cgagtcgtta tgccctctcg tcgccttcgc
     1501 gctaaagacg tcgtggacga gtgcacaacg gattcctttg actttattcg cagcgatcga
     1561 catgccgatc cctgttccgc aaatgaggat gccccggtcg aacttcccgc ttgcgacgcc
     1621 atccgctacc ggtttggcga aatccggata atcgaccgat tcatcggaat ccggaccgaa
     1681 gtcctcatag tccaaaccga gttccgataa taagtccata atttcatggc gcagacggtt
     1741 gccgccatga tccgaagaga ttgcgatttt cattgcgtcc cctcttttct taatttactc
     1801 acaagtcatt ttaccattgt tccataaaaa aacacagccc cggtatgact atttcgggct
     1861 gtgaaatgaa tagcttattc tcatgaacga tcaaattggc tgatgacttt atacaaatct
     1921 tccgattgtt gcttcaatcg gcttgccatc acatcggtct gctcgatcga ttttacttgc
     1981 tcttccgtgg ctgcgcgcac ttcttcggcg cctgccgaag tctcctccgc gatggccgca
     2041 acttcctgcg attgcatggc ggtcgtttcg atattcgata gctggtgttc gacgagctcc
     2101 gaaattttga cgacggaatc ggccatgccg ttcactttct ctgccatcgc ttcgacgttg
     2161 ccgctcgttg catctgcgcg ttccgcttcg ttcgctgccg tgctcacttg cttttccatt
     2221 tccttcacga cttttgagac gtcggactgg atcattttca cgagttcgtc gatgctgtgc
     2281 accgctttcg cactttcatc cgcaagcaca cggacttcct ccgcgacgac cgcgaagcct
     2341 tttccatgct ccccggcacg tgccgcttcg atcgacgcgt tcagcgcaag cagattcgtc
     2401 tggccggcga tgctgccgac gagctggacg atttccccga tcttctgtgc gttctgatcg
     2461 agttcgcgga tcgtgccgag cgactgctcg ctctgattcg acatgtcccg gatccctttg
     2521 a
//
LOCUS       DASULI010000329         4390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_971515, whole
            genome shotgun sequence.
ACCESSION   DASULI010000329 DASULI010000000
VERSION     DASULI010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971515"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFX34_07035"
     CDS             complement(<1..229)
                     /locus_tag="VFX34_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase-like protein"
                     /protein_id="HEX5564698.1"
                     /translation="MEQVLRPIYQERASSPETLGVILVEKRSTGDPITDTFDEIMLII
                     TSNEETPIYTKHYTDGTGKAAMHVISEKQLRK"
     gene            378..749
                     /locus_tag="VFX34_07040"
     CDS             378..749
                     /locus_tag="VFX34_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgzB family protein"
                     /protein_id="HEX5564699.1"
                     /translation="MKPYKNKINRIRSFALSLIFIGVVIMYLGIFFRKNEIIMLIFMV
                     LGLLAILGSTVVYAWIGTLSTRAVQIVCPNCGKATKMLGRVDMCGHCREPLTLDPSLE
                     GKEFDVAYNRPESKKDASDKK"
     gene            complement(789..1232)
                     /gene="perR"
                     /locus_tag="VFX34_07045"
     CDS             complement(789..1232)
                     /gene="perR"
                     /locus_tag="VFX34_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEX5564700.1"
                     /translation="MSEVFLKDALVTLKESGVRITPQRHAILEFLISSETHPTADEIY
                     RALEADFPNMSVATVYNNLRVFRNAGLVKELTYGDSSSRFDFVTHDHYHIICDHCGKI
                     VDFHHPGLEEVEHLASHVTGFEVNSHRLEVYGTCPECAAGANKAK"
     gene            complement(1348..2292)
                     /locus_tag="VFX34_07050"
     CDS             complement(1348..2292)
                     /locus_tag="VFX34_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5564701.1"
                     /translation="MNVLFTFKIKDEQMEKLKADFPGIQFCFNKKIDEAPIGEFETVV
                     TYGEDITTEVLERATSLKWLMVASAGVEKMPLAEIAAKGIVISNVRGIHKTPMAETAL
                     AHILSLGRALPALAEQQKRKEWNTKVRQTELRGSTALILGPGAIGTEIGRLLQAFGVR
                     TIGCNHSGNPAEFMDDTIQFDGLLSRLPEADIVISVLPSTGKTKGLLTENHFKAMKNT
                     AVFMNFGRGDLVKETVIVDALKNGEIGLAVLDVFEKEPLPAESELWSLSNVTISPHVS
                     SHSGKYIERALEFFTENLGKWQNGDKDLINLIDPVKGY"
     gene            complement(2308..2787)
                     /gene="bcp"
                     /locus_tag="VFX34_07055"
     CDS             complement(2308..2787)
                     /gene="bcp"
                     /locus_tag="VFX34_07055"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX5564702.1"
                     /translation="MEKLEGMQAPQFTLPDENGEIVSLRDFEGKKYVILYFYPKDATP
                     GCTTQACDFRDAHQSFTDLNAVILGVSPDSEKSHQRFIEKQGLPFSLLVDENHEAAEA
                     YGVWKLKKMFGKEYMGIERSTFLIDPTGTVVKEWRKVKVKGHIDEALATLEQLTKKA"
     gene            2915..4207
                     /locus_tag="VFX34_07060"
     CDS             2915..4207
                     /locus_tag="VFX34_07060"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5564703.1"
                     /translation="MNRTTSEQIYQEACEHIVGGVNSPSRGYKAVGGGAPTVMERAEG
                     PYFWDVDGNKYIDYLGAYGPIITGHAHPHIAEAITKAATTGVLYGTPTRHEVKFAKML
                     KEAIPSMDKVRFVNSGTEAVMTTIRVARAFTGRTKVMKFAGCYHGHSDLVLVAAGSGP
                     ATLGTPDSAGVPASIAQEVITIPFNDPEAYKEAMEKWGDELACVLVEPIVGNFGIVEP
                     NEGFLPLVHELAKEKGALIIYDEVITAFRFHYGSAQTLLGTTPDLIAFGKIIGGGLPI
                     GAYGGKKEIMETVAPLGPAYQAGTMAGNPASMLSGIACLEVLQQPGIYEEMDRLGGML
                     EEGILQLAEKHGITLTINRLGGALTLFFTDVTVENYEQAEQTDGEIFARFFKLMLENG
                     VNLAPSKYEAWFLTIAHKEEDIVHTLEAVDRSFKALAQ"
     gene            4315..>4390
                     /locus_tag="VFX34_07065"
     CDS             4315..>4390
                     /locus_tag="VFX34_07065"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEX5564704.1"
                     /translation="MKLGARIFKTGIAIVAALFLAEMLS"
BASE COUNT         1124 a         1066 c          977 g         1223 t
ORIGIN      
        1 atttcctcaa ctgtttttcg ctgatgacgt gcattgctgc ttttcctgtt ccatccgtgt
       61 agtgttttgt atatattggc gtttcttcat tagatgtaat gattagcatg atttcatcaa
      121 acgtgtctgt tatcggatcg cctgtactcc ttttttcaac tagaatgaca cctaatgttt
      181 caggcgagct tgcacgttct tggtaaatcg gtcttagaac ctgttccatt atgacacctc
      241 cacctttatt tgtttcgacg cctcttctaa aaaacccttc attcaaaatt cgtaaaatat
      301 gatgcgattg gaatttgtgc tatattgatt gaggaacatt catttcatta ttacattcat
      361 tctaaggagg attatggatg aaaccttata aaaataaaat aaaccggatc cgttcatttg
      421 cgctatccct cattttcatc ggggttgtca ttatgtacct tggaatcttc ttcagaaaaa
      481 atgaaatcat catgttgata ttcatggttc taggtctcct tgccattctt ggcagcacag
      541 ttgtttatgc gtggatcggg acgttgtcga cgagagctgt acaaatcgtc tgtccgaatt
      601 gcggcaaagc gactaaaatg cttggacggg ttgacatgtg cgggcattgc agagaaccgc
      661 tcactcttga tccttctttg gaaggcaagg aattcgatgt cgcgtataac cggccggaaa
      721 gcaaaaaaga tgcatcagat aaaaaataag aaaaccggtt ccgtctttca cactcggaac
      781 cggttttctt attttgcttt attggcgcct gctgcacatt caggacatgt tccatatacc
      841 tcaagacgat gggaattgac ttcgaatccc gtaacgtgtg aagcgagatg ctcaacttct
      901 tcaagtccag gatgatggaa gtcaacgatt ttcccgcaat gatcacaaat gatgtggtag
      961 tgatcatgcg tgacaaaatc gaaccggctt gacgaatctc cataggtgag ctcctttaca
     1021 agcccggcat tccggaaaac acgcagatta ttatatacgg ttgcaacact catgtttgga
     1081 aaatcagctt ccagtgctct atatatttca tcagctgtag gatgcgtctc ggaagaaatg
     1141 agaaattcta atatggcatg cctttgcggt gtaatacgaa caccgctttc cttcaatgtg
     1201 acaagcgcgt ctttcaaaaa tacttcagac atcgccaaca ctccaatcca taaagattac
     1261 ttctttataa tcattataat tagtttactg aggaaaggca tatttgtcaa ctaaagtgct
     1321 gtcctacggg accatgcagg ccatgaatca ataccctttt acagggtcga tcaaattgat
     1381 cagatccttg tctccatttt gccatttccc caggttttcc gtgaaaaatt cgagtgctct
     1441 ctctatatac ttcccggaat ggctggagac atgcggagaa attgtcacat tcgataaaga
     1501 ccaaagctcg ctttccgcag gcagcggttc tttttcaaat acatctagga ctgcaagccc
     1561 gatctcccca tttttcaaag cgtctacaat aaccgtttcc ttcacaagat cccctctgcc
     1621 gaaattcatg aatactgctg tatttttcat cgccttgaaa tgattttccg tcagcaagcc
     1681 cttcgttttt ccggtgctcg gcaatacgga aatgacgata tccgcttccg gcagtctgct
     1741 taatagcccg tcaaattgga ttgtatcgtc cataaattcc gcaggattcc ctgagtgatt
     1801 gcaaccgatc gtcctgacgc cgaaagcctg aagcagccgt ccgatttccg taccgattgc
     1861 acctggtcca aggataagtg ctgtagatcc gcgcaattcc gtttggcgta ctttcgtatt
     1921 ccactctttc cgtttttgct gctccgccaa tgcaggaagc gcccgcccga gtgatagaat
     1981 atgggcaagc gccgtttccg ccatcggggt tttatggatg ccgcgcacat tgctgataac
     2041 gatccctttt gctgcgattt cggcaagcgg catcttctcc actcccgcag atgcgaccat
     2101 cagccatttt aaagatgtag cccgctcaag cacttccgtc gttatatctt ctccataagt
     2161 gacaacggtc tcgaattcac caatcggggc ctcgtcaatc tttttattga aacaaaattg
     2221 aattccggga aaatccgcct tcaatttctc catctgttca tcttttatct tgaaggtgaa
     2281 taaaacattc atccgccccg ctccccctta cgccttcttc gtcaattgct ccaatgttgc
     2341 aagcgcctca tctatatgcc ctttcacttt cactttacgc cattccttca cgactgttcc
     2401 cgttggatcg atcagaaaag tcgagcgctc gatgcccata tattccttgc cgaacatttt
     2461 ctttaatttc catacgccat acgcttccgc cgcttcgtga ttttcatcca ccaacaatga
     2521 aaatggcaac ccttgctttt cgatgaaccg ttgatgtgat ttctcactgt ccgggctaac
     2581 ccctaaaatg acagcgttca ggtcagtgaa gctttggtgt gcatcccgga aatcgcacgc
     2641 ctgcgttgtg cagccaggag ttgcgtcttt cggataaaaa tagaggatga catacttctt
     2701 cccttcaaaa tcccgaagtg aaacgatttc accgttttcg tccggcaatg taaattgcgg
     2761 cgcctgcatt ccttccaact tttccatagc gatccctcca actttttcta taccccaatc
     2821 gtaccggaca agtgatagag tttcaatttc tttggcgtat ccacgcaagt ttgtataaaa
     2881 tggagattgt accgatttga acggaggaat tgaattgaac agaacaacat cagaacagat
     2941 ctatcaagaa gcatgtgaac atatcgttgg tggcgtcaac agtccatccc gcggttataa
     3001 agcggtcggc ggcggagccc ctacagtgat ggagcgtgca gaaggccctt atttctggga
     3061 tgtcgatggc aataaatata tcgattactt aggtgcatat ggtccgatca tcactggaca
     3121 cgcacatcca catattgcgg aagcaattac gaaggctgcc acaactgggg tgctctacgg
     3181 aacgccgacg cggcatgaag tcaaattcgc caaaatgctg aaggaagcga tcccttccat
     3241 ggataaagtc cgtttcgtca attcagggac cgaagctgtc atgacgacga tccgggtagc
     3301 ccgtgctttc accggaagga cgaaagtgat gaagtttgcg ggttgctacc acgggcattc
     3361 agaccttgta ctcgtagctg cgggttccgg tccggcgaca ttgggcaccc cggattcagc
     3421 aggagtcccg gcttccattg cccaagaggt gatcacgatc cctttcaatg atccggaagc
     3481 gtataaagaa gcaatggaga aatggggaga cgaattggca tgtgtcttgg ttgagccaat
     3541 cgtcgggaat ttcggtattg tcgaaccgaa tgaaggattc cttccgctcg tccacgaact
     3601 tgcgaaagag aaaggcgcgc tcatcattta tgatgaagtg atcacggctt tccgtttcca
     3661 ttatggcagt gcacaaacgt tgcttggcac gactcccgat ttgatcgcct tcgggaaaat
     3721 catcggcggc ggcttgccga tcggggctta tggcggcaag aaggaaatca tggaaactgt
     3781 tgcaccgcta gggcctgctt accaagccgg cacgatggcg gggaatccgg catccatgct
     3841 atccggcatc gcttgcttgg aagtcttgca acagcctggc atctatgagg aaatggaccg
     3901 gcttggagga atgctggaag aaggcatcct tcaattggcc gaaaagcacg gcatcacgtt
     3961 gacaatcaat cgtcttggcg gagcacttac attattcttc acggatgtga cagtggagaa
     4021 ctatgagcag gctgaacaga cggacggaga gattttcgcg agattcttta aactcatgct
     4081 tgagaacggc gtcaacctcg ccccatccaa atatgaagca tggttcctca cgattgcgca
     4141 taaagaagag gacatcgttc atacattaga ggcggtggac cgttcattca aggcgctcgc
     4201 gcaataaatg gtatattgac ccactgtaaa aggtggggta cagtaactga aaacgccttt
     4261 gttcattaat aaggcataat ctttttcaat acctttagta gaaaggataa tcatatgaaa
     4321 cttggcgccc gcatcttcaa aacgggtata gcgatcgttg ccgcattgtt tctggcggag
     4381 atgctcagct
//
LOCUS       DASULI010000330         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_379595, whole
            genome shotgun sequence.
ACCESSION   DASULI010000330 DASULI010000000
VERSION     DASULI010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379595"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1611)
                     /locus_tag="VFX34_07070"
     CDS             complement(28..1611)
                     /locus_tag="VFX34_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX5564705.1"
                     /translation="MNALFKDQRFIDYNASGFRKDLLSGITVGIVAIPLGMAFAIASG
                     VKPEYGLYTTIIAGFLVALLGGSRFQIAGPTGAFIPILLAIVLQYGYEDLLIAGFLAG
                     IFLVIMSLAGVSNLIHFVPRSVTIGFTSGIAIIIFTGQFGNFLGLSGLESKEFFHENM
                     IDVAKHLHTVNLFSVLTAVVGLAMIFILPKVAPRLPLLLFALIIPTIVSVLFYPGKVE
                     TIGTAFGGIPQSLPGLQIPKITLTKLVSLWQPALIIAALGAIESLLSAVVADGMKEGP
                     KHDSKRELFGQGIANIISPLFGGIPATGAIARTATNIRSGAVSPVSGIVQSVFVLLFI
                     LVFAPYASYIPLAAMAPILMFVAWNMSALTAFIHILEVRSSDSIVLLTTFLLTIFINL
                     TVAVQIGILLAAVSFLRKMSGKLHIVPDKLSDVEIHKFGEDVSKFVIEGPLFFGTAKV
                     FEDKMPDMLVQGTSKTILDFEHLSVIDATGEAALSALLDAASKKNNLIIVKKLPKEKL
                     SMFKKSGLYDRIGEENFFM"
     gene            complement(1860..>3229)
                     /locus_tag="VFX34_07075"
     CDS             complement(1860..>3229)
                     /locus_tag="VFX34_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5564706.1"
                     /translation="TGAKFTVYDEIAFGLENQGVPREVMHERIQESMKLLDIEGLSEK
                     NPFSLSGGQMQRVAIASVIAMKPDILILDEPTSQLDPEGAEEVFRVVEKLADNGMTII
                     MVEQKMEKLAAYADRILLMHEGKLIEDGKPEVVFSRDDLHAFGVEPPVYVKIARTLGL
                     RNETTGHYPISLEGMPTERKIQFRAESKISSTGKTTAELIVRDLHFSYEKGQEVICGL
                     NVSLSGEPIAIIGQNGGGKTTFVKLLKALLKPDSGEILLDGAPTSETTAAKLASRIGL
                     IFQNPNDQIFKHKVIDEVMFGPLNIGQSNEEALYNAKKMLELVDLADKAEENPYDLSL
                     AERKMVAMASILAMDTDIVIFDEPTMGQDVRGKAKVQEIIHSLHETGKLVICILHDMD
                     FVADTFGRTIIFSNGGLLFDGPTREAFAQAEALRLARVEQPHITKLVQRLGYDRVVLR
                     ETELV"
BASE COUNT          809 a          876 c          758 g          786 t
ORIGIN      
        1 atccgcgctc aaaaaagtct ttcgtcctca catgaagaaa ttttcttcgc cgatccggtc
       61 atacaatccg ctctttttga acatggagag cttctccttc ggcagtttct tcacgatgat
      121 caggttattc tttttcgacg cggcatcgag caatgcggat agggcggctt cgccggttgc
      181 gtcaatgact gagagatgct caaaatcaag gatcgttttt gacgtgcctt gcactagcat
      241 atcgggcatc ttatcctcaa acactttcgc tgtaccgaag aacaacggtc cttcaatgac
      301 aaacttggat acatcctctc cgaatttatg gatctcgaca tcagaaagtt tatccggcac
      361 gatatggagc ttgccgctca tcttccgcaa gaaggagact gctgcgagca gaatcccgat
      421 ctggacggca acagtcagat tgatgaagat cgtaagcagg aatgtagtca gcagcacgat
      481 cgaatcgctc gatctgactt ccaaaatatg aataaatgct gtcagggcac tcatattcca
      541 cgcgacaaac attaggatgg gcgccatcgc agccaatggg atataagagg catagggtgc
      601 gaaaaccaaa atgaacaata agacgaaaac actttgcaca atgcctgaaa ccgggctgac
      661 agcgcccgaa cggatattgg tcgccgtacg cgcaatcgct cctgttgctg ggatgccccc
      721 gaataacggc gaaatgatat tcgcgattcc ctggccgaac aattcccgtt tggaatcatg
      781 cttcggacct tctttcattc catcggctac gactgccgac agcagcgatt caatcgcgcc
      841 gagcgccgct atgatcaacg cgggctgcca aaggcttacg agtttcgtca atgtgatttt
      901 tggaatttgc agtcccggta acgattgcgg aataccgccg aacgccgtgc cgatcgtttc
      961 cacttttccc gggtaaaaca ggacagacac gatcgttgga atgatgagag caaagagcag
     1021 caacggaagt cggggggcga ctttcggcaa aatgaatatc atggctaggc caaccactgc
     1081 ggtgagcaca ctgaagagat tcaccgtatg aagatgcttg gcgacatcaa tcatattttc
     1141 atggaagaat tccttgcttt ccagtccaga caggccaagg aagttcccga attgccccgt
     1201 gaaaatgatg atggcgattc ccgacgtgaa cccgatcgtc acggaacggg ggacgaaatg
     1261 aatgagattg ctaacgcctg ccaaactcat gatcactagg aaaatacctg ctagaaagcc
     1321 tgcgatcaac aggtcttcat agccgtattg aaggacgatg gcaaggagga tcgggatgaa
     1381 tgcgccggtc ggtccggcaa tctgaaagcg agagcctccc aataaggcga ctagaaaacc
     1441 tgcaatgatt gtcgtataca agccatactc cggtttaaca cccgaggcaa ttgcaaaagc
     1501 catcccgagt ggaatggcta cgataccgac agtgatcccc gaaagcagat ccttccgaaa
     1561 tcctgacgcg ttataatcaa tgaaacgttg gtctttgaac aaggcgttca ctcttcttcc
     1621 acagtatttg ttaaaatatt tcctttcttc attataagcc acaaacacag tgctggcaag
     1681 gtttcgcgtc caaagccgac gatctcgcgc ccaatgtaga tggttgcgag tccaaagcag
     1741 gcgttcccgc gtccaaagca agtggctgcg cgtccaaagc gggtggtccc acgtccaaag
     1801 caggtgctcc ggcgtccaaa gcagcgcctc ccacgtccaa agcgcagccc acaatgaatc
     1861 tataccaatt cggtctccct taatacaaca cgatcatagc ccaatcgctg cactagcttc
     1921 gtaatatgcg gctgttcgac ccgcgcgagt cgcaatgctt ccgcttgcgc aaacgcctcg
     1981 cgtgtcggcc cgtcgaacag caatccgcca ttgctgaaaa tgattgtccg accgaacgta
     2041 tccgcaacga aatccatgtc atgcaaaatg cagatgacga gcttgcctgt ttcatggagc
     2101 gagtggatga tttcctgcac tttcgccttc ccgcggacat cctgccccat cgtcggttca
     2161 tcgaaaatga cgatgtcagt atccatggct agaatggaag ccatcgcgac catcttccgt
     2221 tcagcgagac tcaagtcata cggattctcc tccgctttat ccgccaggtc gacgagttcc
     2281 agcatctttt ttgcgttata cagtgcttcc tcattggatt ggccgatgtt caacggtccg
     2341 aacatcactt catcaatcac cttatgcttg aaaatctggt cattcggatt ttggaagatg
     2401 agcccgatcc ggcttgccaa tttagctgca gtcgtttcgg atgtcggtgc tccatccaat
     2461 agaatctcgc cggagtccgg tttcaacaat gccttcaata atttcacaaa tgtcgttttc
     2521 ccgccgccat tctgtccgat gatcgcgata ggttcgccgc ttaacgagac attcagcccg
     2581 cagatgactt cctgtccttt ttcatagctg aaatgaagat cccggacaat gagttcagcc
     2641 gtcgttttgc cggtcgaact gattttactt tctgctcgaa attgaatttt acgttccgtc
     2701 ggcattccct ccaatgaaat cggataatgc cctgtcgttt cattcctcag gccgagagta
     2761 cgtgcaattt tcacgtagac gggtggttcg acgccgaagg catgcaaatc atcccgtgaa
     2821 aagacaactt caggtttccc atcctcgatc aacttgcctt catgcatcaa caggatcctg
     2881 tccgcatatg ccgcgagttt ctccattttc tgctccacca taatgatcgt catgccgttg
     2941 tccgccaact tttcaacgac gcggaacact tcctccgcgc cttccggatc gagctgggat
     3001 gtcggttcgt caagtattag gatgtcaggc ttcatggcga tgacgcttgc aatggcgacc
     3061 cgctgcattt gcccgccaga taatgaaaag ggattcttct cactcagtcc ctcgatatcg
     3121 agcagcttca tgctttcctg gatgcgctca tgcatgacct cccgcggaac cccttggttt
     3181 tccaagccga atgcaatttc atcatagacg gtgaacttgg cgccggtca
//
LOCUS       DASULI010000331         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1352548,
            whole genome shotgun sequence.
ACCESSION   DASULI010000331 DASULI010000000
VERSION     DASULI010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352548"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..477
                     /locus_tag="VFX34_07080"
     CDS             106..477
                     /locus_tag="VFX34_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564707.1"
                     /translation="MDNKLWKIGFFTGLTSFVLLILGIRTILGTELVFKNYLTFGIFG
                     LTVGILASSLLFYKMKIAFRIFMVALVLGFAEFFRSFLMDTNGFGDLIGVLSLFIITT
                     FGFGLGVIVQFIIIGVKKLKE"
     gene            619..2040
                     /locus_tag="VFX34_07085"
     CDS             619..2040
                     /locus_tag="VFX34_07085"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HEX5564708.1"
                     /translation="MKRRTAEPYKIKSVETLAMLSYEERKESLERAGYNTFLIDSQDV
                     YIDLLTDSGTTAMSDAQWGALMTGDEAYAGSKSWKRLEQAVRDVYGYRFVLPTHQGRG
                     AENILSQLKIKEGDYVPGNMYFTTTRAHQEMNGATFVDIIIDEAHDPSIDLPFKGNVD
                     LDKLRNLIQQVGAEKIPYVCLAVTVNLAGGQPVSMQNMKEVYELCKSYNIDVMLDATR
                     CVENAYFIKEREDGYADRPVADILKEMMSYSDGCTMSGKKDCLVNIGGFLAMNDEDLY
                     IRSRELVVVYEGMPSYGGMAGRDMEAMAIGIREAVDDHYIEHRIGQVRYLGQQLLDAG
                     IPIVQPIGGHAVFLDARAFLPHLSQDEFPAQALAAALYLDSGVRSMERGIISAGRNSK
                     TGEHNRPNLELVRLTIPRRVYTNNHMDVVVDSILALYEKRDAICGLNMVYEPPTLRFF
                     NARFAPLSENGELISEENRKINA"
     gene            complement(2149..3216)
                     /gene="rlmN"
                     /locus_tag="VFX34_07090"
     CDS             complement(2149..3216)
                     /gene="rlmN"
                     /locus_tag="VFX34_07090"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5564709.1"
                     /translation="MRKSIYGLTLDQLTNWLVENGQKKFRAAQVWDWLYKKRVTNFAD
                     MSNINKECLQLLEENFVIQTLEQSVKQVSEDGTIKFLFKMQDGNLIETVLMKFPYGYS
                     VCVTTQVGCNIGCSFCASGLLKKNRDLDAGEIVGQIMKVQQHLDEVGKGERVSHIVVM
                     GIGEPFDNYVNLMNFLRVINDQKGLSIGARHITVSTSGLTPKIREFAEENIQINLAVS
                     LHAPNNELRTNIMKINKAYPIEKLMEAIDYYLETTNRRITFEYILLDDVNDHIAEAEQ
                     LAKLLYNKRHLSYVNLIPYNPVDEHGQYQRSKPEAIKSFYEALKKRGINCGVRWEQGS
                     DIDAACGQLRSKQIKKEKVAK"
     gene            complement(3303..3494)
                     /locus_tag="VFX34_07095"
     CDS             complement(3303..3494)
                     /locus_tag="VFX34_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564710.1"
                     /translation="MTTPKFIDIALIIFLSYFAFDRFSKGMTGIGIFFTVLALMNVFV
                     LVMKIKQDKMRRTSQNNQS"
BASE COUNT          929 a          804 c          876 g         1075 t
ORIGIN      
        1 ggtgccttct ctttcaggga ggagcagccg cttttttccg catctatact ctacactttg
       61 ctcccttttt ccgttatgat atgtactgaa agcgggtgca cttcattgga caataagtta
      121 tggaaaatag gtttttttac aggtctgact tccttcgtac tgcttatctt agggattcgg
      181 accattttag ggacagaatt ggtgttcaaa aattatttga cgttcggcat attcggtttg
      241 actgtcggca tcctggcatc ttccttactt ttctataaga tgaagatcgc cttccggatc
      301 ttcatggtcg ccctcgtttt aggcttcgcc gaatttttcc gaagtttcct catggacacg
      361 aacggatttg gagacctgat cggtgtcctt tcattattca tcattacgac attcgggttc
      421 ggacttggcg tcatcgtaca gttcattata atcggcgtca aaaaactaaa ggaataggaa
      481 tggatgaagg gccgcctcat atatatggag tgcggtcttt ttttataata gagagaaact
      541 attgtaaatt aattgaatag taggtttcca agggatatag tgaaatgggg agaatctttt
      601 taaaagggga tgtttgtatt gaaaagaaga actgccgagc cttataagat taaaagtgtg
      661 gagacgcttg ccatgctatc ttacgaggaa cgaaaagagt cacttgaacg ggcgggatat
      721 aacacgtttt tgatcgactc gcaagatgtg tacatcgatc tgttgacaga cagcggaacg
      781 acggcaatga gtgacgcgca atggggagcg ctcatgacgg gtgacgaagc ctatgccgga
      841 agcaagagct ggaaacggct ggagcaggcc gtccgtgatg tatacggata tcgatttgtg
      901 ctgccaacac atcaaggccg cggtgcggag aatattttgt cgcagctgaa aatcaaagaa
      961 ggcgattatg tgcctggcaa tatgtatttt actacaacgc gtgcacacca ggagatgaac
     1021 ggcgctacgt tcgtcgacat cattatcgac gaagcgcatg acccatccat cgatctccct
     1081 ttcaaaggaa atgtcgattt ggataaactc cgaaatttga ttcaacaagt cggcgccgaa
     1141 aagattccgt atgtctgtct tgccgtcacg gtgaatcttg caggcggcca gcctgtcagc
     1201 atgcaaaata tgaaagaggt ctatgaactg tgcaagtcct acaatatcga cgttatgctg
     1261 gatgcaacgc gttgcgttga aaacgcttac tttatcaagg agcgggagga tggttatgcg
     1321 gaccgtcctg tagctgatat cttgaaggaa atgatgtcct attccgacgg ctgcacgatg
     1381 agcgggaaaa aggattgtct cgtgaatatc ggcggattcc tcgcgatgaa tgatgaagat
     1441 ttatatatcc ggtcacgcga gctagtggtc gtctatgaag ggatgccttc ctacggcgga
     1501 atggctggcc gggatatgga agcgatggcg atcggcatcc gggaggcggt cgatgaccat
     1561 tatatcgagc atcggatcgg tcaggtgaga tatcttgggc aacagctttt ggatgctggc
     1621 attccgattg tacaaccgat cggcggccac gcagtatttt tggatgcgcg cgctttcctc
     1681 ccccacttga gccaagatga gtttcctgcg caagcgctag ccgctgcact ctatttggat
     1741 tccggcgttc gcagcatgga gcgcggaatc atatcggctg ggcggaatag caagaccggt
     1801 gagcataatc gcccgaatct tgaattggtg cgtctgacga taccgcgaag ggtgtatacg
     1861 aataatcata tggatgttgt cgtggattcg attttggcgt tatatgagaa aagggatgca
     1921 atttgtgggt tgaacatggt ttacgagccg ccgacgcttc ggttcttcaa cgcgcgattt
     1981 gcgccgctgt cggaaaatgg agagttgatc agtgaagaga atcggaagat taacgcgtag
     2041 acccgtgaat tgagcccttg tgcgattaat tgagcccttg gcggaataat ggagcccttt
     2101 agcaaaacaa aaacgcatgc cctttttgcc ggcatgcgtt cttcgtattt atttcgccac
     2161 tttttctttc ttgatttgct tgcttcgcaa ttgtccgcaa gctgcgtcga tatcggaacc
     2221 ttgttcccat cggacgccgc agttgatgcc gcgttttttc aatgcttcat aaaacgactt
     2281 gattgcttca ggtttgcttc gttggtattg accgtgctca tcgaccgggt tgtacgggat
     2341 gaggtttacg taagacaaat gacgtttgtt ataaagcagt ttcgccaatt gttcagcttc
     2401 tgcgatatga tcattcacat catccaatag gatgtattcg aatgtgatcc ggcggtttgt
     2461 cgtttccaaa taataatcga ttgcttccat cagcttttca atcggatatg ctttgttgat
     2521 tttcataatg ttcgtccgta gttcattatt tggcgcatga agggagaccg ccaaattgat
     2581 ctggatgttt tcttcggcga actcacggat cttcggtgtc aaaccgctcg ttgaaacggt
     2641 aatgtgacgt gcaccgatcg ataaaccttt ctggtcgttg atgacgcgga ggaagttcat
     2701 cagattcaca tagttatcga acggttcgcc gattcccatg acgacgatat ggctgactcg
     2761 ttcccctttg ccaacctcgt caagatgctg ctgcactttc ataatttgac cgacgatttc
     2821 cccggcatcc aaatccctgt ttttcttcag taaaccgctg gcgcagaagc tgcatccgat
     2881 attgcatccg acttgcgtcg ttacacatac tgaatagccg tacgggaatt tcatcaaaac
     2941 agtttcaatc aaatttccat cctgcatttt gaaaaggaat ttgatcgtgc catcttccga
     3001 aacttgcttc accgactgtt caagcgtctg tattacaaaa ttctcctcca ataattgaag
     3061 gcattcttta tttatattcg acatatctgc aaagttggtg acacgctttt tgtaaagcca
     3121 gtcccacact tgtgcagcac ggaatttctt ttgtccattt tcgactagcc aatttgtcag
     3181 ttgatccaat gtcaagccgt aaattgattt tctcatgtca attcctcact tctcagattt
     3241 gcattcagtc attataccat ggaatggata aaaacagaag aaaaaagctg cattatacaa
     3301 tgtcagcttt ggttattttg gctcgtccgc ctcattttat cctgttttat tttcatgacg
     3361 agaacgaata cattcatcaa tgccaaaaca gtgaagaaaa taccgatgcc tgtcatccct
     3421 tttgagaaac gatcaaaagc aaaatagctt aagaagatga tcaacgcgat atcgatgaat
     3481 tttggtgtag tcaactggat gctgcctcct ttatgccaat cattccattt gacatcagta
     3541 tagcagttcc gtatccccca ttgccatcct cttttttatt tacagccggc acccgtcaag
     3601 caattgttcc agtttcaata acagccgatc catctgttca tccgttccaa tcgaaatgcg
     3661 caaatagttt tcgatgcggg gttg
//
LOCUS       DASULI010000332         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1917144,
            whole genome shotgun sequence.
ACCESSION   DASULI010000332 DASULI010000000
VERSION     DASULI010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917144"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..326)
                     /locus_tag="VFX34_07100"
     CDS             complement(102..326)
                     /locus_tag="VFX34_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564711.1"
                     /translation="MKKEKEVKETLKVQEVDIRKIENRQNGSLSAGVALASSALAPAG
                     SSANAFPAGVSPYLAIFYIHLFIYQVINNE"
     gene            complement(304..573)
                     /locus_tag="VFX34_07105"
     CDS             complement(304..573)
                     /locus_tag="VFX34_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEX5564712.1"
                     /translation="MSPLKVSSRSNPNSVAGALVAVIRDQGYAEMQAVGAGALNQAIK
                     AVAIARGFVAPSGSDLVCAPAFTDIEINGEGRTALKLLVEKRKRS"
     gene            complement(661..1458)
                     /locus_tag="VFX34_07110"
     CDS             complement(661..1458)
                     /locus_tag="VFX34_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX5564713.1"
                     /translation="MRVIFIGDIVGSIGRDMVFDHIARLKRKYSADVVIANGENAASG
                     RGITKAIFDDLLRSGVDVVTMGNHTWDQKEIYDFIDETDYLIRPANFSEEAPGKGMTT
                     ITKNGVTLSVINLHGRTFLPPHGDPFEKAEEFIEEASKISPLIFVDFHAEATSEKIAM
                     GWHLDGKASVVVGTHTHVQTADDRILPGGTAYLTDAGMTGPYDEILGMKKEDVIYRFR
                     TNLPVRFEVPKRGRAQLNGVFVELDDQTGKAVAIERIMINEDHRFEP"
     gene            complement(1540..3105)
                     /gene="rny"
                     /locus_tag="VFX34_07115"
     CDS             complement(1540..3105)
                     /gene="rny"
                     /locus_tag="VFX34_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5564714.1"
                     /translation="MPEIINIIISALAGLFVGAVVIYLVIKKVNESRVTGAKNSAEQI
                     IEDAKREAEAMKKEALLEAKDENHKLRIEAESEVRERRAELQKQENRLLQREENLDRK
                     DDALNKREAGLERKDDALSSRQQHIEQMERKAEDLVATQQTELERISALTRDEAKRII
                     LDDVEKELSTDIAVMTKESEQRAKEESEKKAREILSLALQRFAADHVAETTVSVVNLP
                     NDEMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPVRRETARLALEKLVQ
                     DGRIHPARIEEMVDKSRREVDELIRDKGEQTTFDVGVHNLHPDLIKILGRLHFRTSYG
                     QNVLKHSVEVAYLAGLLAAEVGEDVTLARRAGLLHDIGKAIDHEVEGSHVQIGVELAT
                     KYKEHPVVINSIASHHGDEEATSVIAVLVAAADALSAARPGARSETLENYIRRLQKLE
                     EISESYEGVEKSFAIQAGREVRIIVRPDQIDDITAHRLARDIRKRIEEELDYPGHIKV
                     TVIRETRAVEYAK"
BASE COUNT          799 a          901 c          758 g         1000 t
ORIGIN      
        1 caatcaacag aacacgtcag caaataaaat atcagtaacg aagttcttta caatcaattg
       61 caagaataga aaaacgagtg aaggcatgtt ttttcgcact ttcactcgtt atttattact
      121 tgatatataa ataggtgaat atagaaaatc gccagataag gcgagactcc tgcgggaaaa
      181 gcgttagccg aagaccccgc aggcgcgagc gccgaggagg ctaaggcaac gcccgcggaa
      241 agcgagccgt tctggcgatt ttcgatcttt cgaatatcta cttcttgaac tttaagcgtt
      301 tctttaactt ctttttcttt tttcaactaa cagctttagc gctgttcttc cttcgccgtt
      361 gatttcaata tcggtaaatg ccggggcgca aacgaggtcg cttccgcttg gagcgacgaa
      421 gccgcgtgcg attgcgaccg ctttgatggc ctggttcaac gcgccggcac cgaccgcctg
      481 catttccgca tacccttgat ccctgatcac cgctacgagc gctcctgcga cggaattcgg
      541 attagagcgg gatgatacct tcaatggact caccatgttt cctccttgtt ttcacgatgt
      601 ctatggaatg tatttatcca taagtcatct tatgaaaaga aggagatgat tagactcact
      661 tcatggttcg aatcgatgat cctcattgat cataatccgc tcgatcgcga cagccttccc
      721 tgtttggtca tccaactcga cgaacacgcc attcaattgg gcacgtcctc ttttcggcac
      781 ttcaaaccgg acaggcaaat tcgtacggaa tcggtagatg acatcctctt tcttcatccc
      841 caatatttca tcataaggac ctgtcattcc cgcatccgtc aaataggcgg ttccgcctgg
      901 caaaatacga tcgtccgccg tttggacatg tgtatgcgtc ccgaccacga cggatgcctt
      961 gccgtccaga tgccatccca tggcaatctt ttcacttgtc gcttccgcat ggaaatcgac
     1021 aaagatgagc ggagatatct tgctggcctc ctctataaat tcttccgcct tttcgaacgg
     1081 atcgccgtgg ggaggaagaa acgtgcggcc gtgaagattg atcactgata atgtgacacc
     1141 gtttttcgta atcgtcgtca tccctttacc gggcgcttcc tccgaaaaat tcgcaggacg
     1201 gatcaagtag tcggtttcat cgatgaaatc atatatttcc ttttgatccc acgtatgatt
     1261 gcccattgtt acgacatcga ctcccgaacg gagaagatca tcaaaaatcg cttttgtgat
     1321 tcctcttcca gaagcggcat tttcaccgtt tgctatgact acatccgcac tgtatttccg
     1381 tttcaagcgg gcgatatgat cgaataccat atccctaccg atcgatccga caatatcacc
     1441 tataaaaata accctcataa ttgcaacctc tttctacttt tacgaacttc attgaaataa
     1501 aatgactcct gaaccatggc tggacaggag tcattttcat cattttgcat attcgactgc
     1561 gcgcgtttcg cgaatgaccg tcactttgat gtgacctggg taatcgagtt cctcctcgat
     1621 ccgtttccgg atatccctgg ccaggcgatg cgctgtaata tcatcgatct gatccggacg
     1681 gacgatgatc cggacttcgc ggcccgcttg gatggcgaat gatttttcca cgccttcgta
     1741 cgactcggaa atctcttcga gtttttgcag cctacgaatg tagttttcca gcgtttcact
     1801 acgtgcacct ggtcttgccg ctgatagcgc atctgctgca gctaccaata ctgcaatgac
     1861 cgacgttgct tcctcatcac catgatggga ggcgatactg ttgatgacaa cagggtgctc
     1921 cttgtacttc gtcgcgagct caaccccgat ttggacatgg cttccctcga cttcatggtc
     1981 gatcgcctta ccgatatcgt ggaggagtcc tgcacgtctt gcaagggtga catcttcgcc
     2041 aacctcggca gccaacaatc ccgcaagata agcgacttca acagagtgct tcaggacgtt
     2101 ttgtccgtag cttgtgcgga agtgcaatct tccaaggatt ttgatcaagt ccggatgcag
     2161 attatggacg cctacatcaa atgtagtctg ttccccttta tcgcggatca gttcatccac
     2221 ttccctgcgt gacttatcca ccatctcttc aatccgtgca gggtgaatcc tgccatcttg
     2281 cacgagcttt tcaagtgcga gacgcgcagt ttcccgacgt acaggatcga agcctgaaag
     2341 gatgaccgct tccggagtgt cgtcgatgat caaatcgata ccagtcaatg tttccaatgt
     2401 cctgatattc cgaccttccc ttccgatgat tcggcctttc atctcatcgt ttggcaagtt
     2461 cacgacagat acggttgttt ctgcaacatg gtctgctgcg aaacgttgca atgcaaggga
     2521 aaggatttca cgggctttct tttcggattc ctcttttgca cgctgctccg attctttcgt
     2581 cattacggcg atatctgtgg aaagttcctt ctcgacatca tccaggatga tccgttttgc
     2641 ttcatcccgc gtgagggccg agattctttc aagttccgtc tgttgcgtgg caacaagatc
     2701 ttccgccttg cgttccattt gttcaatatg ctgttgtcta ctggaaagcg catcatcttt
     2761 gcgctccagc cccgcttctc ttttattgag agcatcatcc ttgcggtcaa gattttcttc
     2821 ccgttgcaaa aggcggtttt cctgtttttg cagttccgct cttcgttcac ggacttctga
     2881 ttccgcttca attctcagtt tgtgattttc atctttcgct tctaacagtg cctctttttt
     2941 catagcctcc gcttcacgtt tcgcatcttc gatgatttgt tcggcagagt ttttggcacc
     3001 cgtcactctt gattcattca cctttttgat aactaaatag ataacaactg cacctacgaa
     3061 tagaccggcc aaagcggaga tgatgatgtt gatgatttct ggcatccagg tcacctcctc
     3121 ctgctattct tttcgttttc agtcggctcg tatattaaca ttcaacatac tgccataatg
     3181 atttcattga tgtgaaatta tacaattcta attgtatcca tgaaattttg acatgtcaag
     3241 gattgtaaac aaaagtggag ggtggcgttt agcttcgaca ggcataaggc agatctgcga
     3301 aacggcgctc tttgccgtac agcagagctg acttatgacc cgagcggctg ccacccggag
     3361 tctagacgta aacaaaagcg aagggtgcct gcagagacga caggcataag gcggagatgc
     3421 gccgtggcgg tttttgccac agagcaactt tgacttat
//
LOCUS       DASULI010000333         4559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_341416, whole
            genome shotgun sequence.
ACCESSION   DASULI010000333 DASULI010000000
VERSION     DASULI010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341416"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFX34_07120"
     CDS             <1..672
                     /locus_tag="VFX34_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564715.1"
                     /translation="MIMNKRWIPIIILLSALSLGACAGKAESPEQSVTEPVKTTKKED
                     IAKIEGTTFTLKDMEFYSLMQVANLELARARDTENLKDTELEKRNAYWDEEISRFENQ
                     NIQLQKLIEIHSMSLLGKEKNYYIPPDKLEKEIADFKNAIDGNETIKALLEEFGEDEV
                     NGRLHVYFEKVMLVNRIVAEIEQKAREEFPEALDQEIAYEVSERYEELYIDQVSGLER
                     EVYMR"
     gene            complement(765..2414)
                     /locus_tag="VFX34_07125"
     CDS             complement(765..2414)
                     /locus_tag="VFX34_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5564716.1"
                     /translation="MKTKFNFLLVLSLVMAVFLSGCGFTDSKKDNDGEKAGETNEKKE
                     KVVNLSLANDIPDLNQVLTTDGISFSVLNNVMEGLYRLDENNEPQPAMAKDVDVSDDK
                     LTYTFHLRDGIKWSNGDPVTSEDFKYSWLRAMHPDTAGAYSNILSDYIVGGQEFVDGE
                     ADASAVAIETPDEKTLVVTLKTPTPFFLGLTAFVTYFPLNEKFINEVGEDKFALKADA
                     ILYNGPYVMTEYNQAQGVTLVKNKEYWDADNVDVDKVTMKVIKEQSTALNLYEAGELD
                     RVYLASSDVDSYKDSEEFGTETEFISWYLQFNFLEKPFDNVNIRKAFQLAYDAETLTK
                     TVLNNGSVPAYGLVAAGVYGDGDKTFRELSGDHVKMDVAKAKEYLEKGLAEVGELPPL
                     KILTADDTIAKDTAIFLQSEFKKNLGIDVQIDTKPYSGRLDAMRANEYQMGISRWGAD
                     YNDAMDILSLWIGEPLRGLRGNYYNEKYKQLIDDALVEADENKRLQMLIDAEKLMLVD
                     DGTLGPLYFEGKAFLQKPNVKGVIIHPYGASIELKTIKVED"
     gene            complement(2440..3402)
                     /locus_tag="VFX34_07130"
     CDS             complement(2440..3402)
                     /locus_tag="VFX34_07130"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5564717.1"
                     /translation="MKLIDLHCDVLARMHKAKGNLSFNDSNDLDVTYEKLKRGGVHIQ
                     AFAIFISPSMKSDQKFQAALDQIHYFYTEVIGKNPHLKALKTWGDFEQLKDGQIGAFL
                     TLEGVDCIGNDMQKLSILHELGVRSVGLTWNNANLAADGVGEERGAGLTAFGKEIIGF
                     CNENKILSDVSHLSEKAFWDVMDLAHFPIASHSNSKTLCDHRRNLTDEQAIHLFRSNG
                     LIHVVYNPPFVIEKGDASIADLITHIDHFCSLGGVGQIGLGSDFDGISEKIIGLEDAS
                     MQPALINELLKKYSEDEVKGFAYQNFLDHLPGRNAMQPEEEVLS"
     gene            complement(3389..4114)
                     /locus_tag="VFX34_07135"
     CDS             complement(3389..4114)
                     /locus_tag="VFX34_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019721259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5564718.1"
                     /translation="MKPVIGITSNIDSAAHTLQNTYIQAVISGGGIPFVIPTGVENDA
                     HQITSLLDGLIISGGGDMNPILFNEEPLPQLGNVSPERDSAELELVRHMLSADKPILG
                     ICRGHQVLNVALGGTLYQDITSQCTSPVLQHVQKAKRDHQSHAVHIEKGTILASIAIS
                     GKIMVNSFHHQALKDIPAPLIVSGKASDGIVEAVESTAHHFVVGVQWHPEALMQNEDQ
                     VSMRLFEAYMKACAERMAAVETH"
     gene            4383..>4559
                     /locus_tag="VFX34_07140"
     CDS             4383..>4559
                     /locus_tag="VFX34_07140"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX5564719.1"
                     /translation="MSEVLVTVERGQLVESLHRGDIAIVSANGDVISSVGDIEKVVFA
                     RSSMKPLQTIPIIET"
BASE COUNT         1168 a         1057 c         1072 g         1262 t
ORIGIN      
        1 atgatcatga ataaaaggtg gatacccatc atcattctgt tatcggcact atcgttgggc
       61 gcgtgtgcag ggaaagcgga atcgcccgaa caaagcgtga cggagccggt aaaaacaacg
      121 aagaaggaag atatcgcgaa aattgaaggc acgacattta ccttgaaaga tatggagttt
      181 tattcgctca tgcaagtcgc caatttggaa ctggcgaggg cgagagatac ggaaaatttg
      241 aaagacacgg agcttgaaaa acgtaatgcc tattgggatg aggaaatcag ccgttttgag
      301 aatcaaaaca tccagctgca aaagctgatt gaaatccatt ccatgtcatt gttagggaaa
      361 gagaaaaatt actatattcc accggataaa ttggaaaagg aaattgctga tttcaaaaat
      421 gcaatcgatg gcaatgaaac aatcaaggcg ttgctcgagg agttcgggga agatgaagtg
      481 aatggccgcc tccacgttta ttttgaaaag gtcatgctgg tgaaccggat tgttgcggaa
      541 attgaacaaa aggcgagaga ggaatttccg gaagcgttgg atcaagaaat cgcttatgaa
      601 gtgagcgagc gatatgagga attgtatatc gaccaagtat ccggtttgga aagggaagtc
      661 tatatgcgtt aacaagagtc aggaccggta gataggttcc tggctcttgt ttgttttgat
      721 ccaaaagaaa caacccgctt agcaacgtaa gcgggcaggt tcaatcagtc ttcgacttta
      781 attgttttca attcaatgga agccccatac gggtggatga tgacaccttt tacattcggc
      841 ttttgcaaga atgcttttcc ttcgaagtat aacggaccta atgtgccgtc atcgaccagc
      901 attaattttt ccgcgtcgat caacatttga aggcgtttgt tttcatccgc ttcgacaagt
      961 gcgtcatcga tcagctgctt atacttttcg ttgtagtagt ttccgcgaag tccgcgaagc
     1021 ggttccccga tccataggct taaaatatcc attgcatcgt tataatccgc accccatcgg
     1081 gaaattccca tttggtattc gttcgccctc atcgcatcca agcggccgct atacggtttc
     1141 gtatcgattt gtacatcgat gccgagattc tttttgaatt cactttggag gaagatcgct
     1201 gtgtctttcg caatcgtgtc atccgcagtc aaaattttaa gcggtggaag ttcgccgact
     1261 tcggcaagcc ctttttccaa gtactctttc gctttggcaa cgtccatttt cacatggtct
     1321 ccgctcagct cgcggaatgt tttatcgcca tcgccgtaaa cacctgctgc gaccaatccg
     1381 taagctggga cggaaccgtt gttcagaacc gtttttgtca atgtctccgc gtcatatgca
     1441 agctggaagg ctttacggat attgacattg tcaaatggct tttcaaggaa gttgaactgc
     1501 aagtaccagg aaatgaattc cgtttccgtg ccgaattcct cgctgtcctt gtaggagtcg
     1561 acgtctgaag acgccaagta gacacggtcc agctcgccgg cttcatatag attcaatgcg
     1621 gtgctttgct ctttgatgac tttcatcgtc actttgtcga catcgacgtt gtcagcatcc
     1681 cagtactctt tgtttttcac taatgtgacg ccttgcgctt ggttgtattc ggtcatgacg
     1741 tacggtccgt tatacaagat cgcatccgcc ttcaatgcga atttatcttc gccgacttcg
     1801 ttgatgaatt tctcgttcaa cgggaaatac gtgacaaaag ctgtcaaccc aaggaaaaac
     1861 ggtgttggtg ttttaagggt gacgacaagc gtcttctcgt cgggcgtttc aatcgcgact
     1921 gctgacgcgt cagcctcgcc gtccacaaat tcctgtccgc cgacaatata atcggaaagg
     1981 atattggaat aggcgccagc cgtatccggg tgcatcgccc gcaaccagga gtatttgaag
     2041 tcctcggaag tgacagggtc tccattgctc catttgatgc catcgcgcaa gtggaatgta
     2101 tacgtcaatt tgtcgtcgga aacatcgacg tcttttgcca tggcaggctg tggttcgttg
     2161 ttttcatcca aacggtaaag cccttccatc acgttgttca acacggagaa tgaaataccg
     2221 tccgtcgtca gcacttggtt caagtcggga atatcattgg caaggctcaa atttacgact
     2281 ttttcctttt tctcattcgt ctcgccggcc ttttcaccgt cattgtcctt cttcgagtct
     2341 gtgaagccgc aaccgcttaa aaagacggcc atgaccaaag agagtacgag taaaaaattg
     2401 aatttcgttt tcattggaat ccccctattc tacttattct taagaaagca cttcttcctc
     2461 aggttgcata gcattcctgc cgggaaggtg gtcaaggaag ttctggtatg cgaatccttt
     2521 cacttcatcc tcgctatact tttttaacag ttcattaatc aaagcgggct gcatcgaggc
     2581 atcttcaaga ccgatgatct tttccgaaat gccgtcaaaa tccgacccca atccaatctg
     2641 gccgacaccc cccagagaac agaaatggtc aatatgcgtg atgagatctg cgatggatgc
     2701 atcacctttt tcgatgacga aaggcgggtt atacacgacg tggatcagac cattcgatct
     2761 gaataaatgt atggcctgct catccgtcaa gttccgccga tggtcgcaga gcgtcttcga
     2821 attggagtgg cttgcgattg gaaaatgggc caaatccatg acatcccaaa aagccttttc
     2881 gcttagatgc gaaacatccg agagaatttt gttttcattg cagaacccga taatttcctt
     2941 cccgaatgca gtcaggccag cgccgcgctc ttctccgaca ccatccgccg ctaagttcgc
     3001 gttattccac gtcaatccga ccgaacggac gccgagctca tgaagaatcg acagcttttg
     3061 catgtcgttc ccgatgcaat cgactccttc cagcgtcaga aaagcgccga tctgtccatc
     3121 cttgagctgt tcaaaatcgc cccacgtttt taaagctttc aaatgcggat ttttgccgat
     3181 cacttcggtg taaaaataat ggatctgatc gagggccgct tggaatttct ggtccgattt
     3241 catggacggt gagatgaaaa tggcgaatgc ctggatatgg acgccgcctc gcttcagctt
     3301 ttcgtatgtg acatcaagat cattggaatc gttgaatgat agattccctt ttgctttatg
     3361 cattcgggcc aatacgtcgc aatgcaggtc aatgagtttc aacggcagcc atcctctccg
     3421 cacatgcttt catataggcc tcgaataggc gcatggaaac ctgatcttca ttttgcatca
     3481 gagcctcggg atgccattgg acgccgacga cgaaatggtg agccgtactt tcaaccgcct
     3541 ctacaattcc gtcactggcc ttgcccgata cgatgagcgg tgcagggata tccttcaatg
     3601 cttggtgatg gaatgaattc accatgattt tcccggagat tgcgatggac gccaggatag
     3661 tgcctttttc aatatggacc gcatgggact gatgatccct tttcgctttt tggacatgct
     3721 gcaataccgg actggtgcat tgcgaggtga tgtcctggta aagcgttccg cctaatgcga
     3781 cattcagcac ttggtggcct ctgcatatgc caaggatcgg cttatccgcc gaaagcatat
     3841 gtctgacgag ctccaattcg gccgagtccc tttcggggga gacgttccca agctgcggca
     3901 gaggctcttc attgaaaagg atgggattca tatcgcctcc gcctgaaata atcaatccgt
     3961 caaggagtga agtgatttgg tgtgcatcat tttccactcc tgtcggtatg acaaaaggta
     4021 tgccgccccc ggaaatgacc gcttggatat acgtgttttg aagcgtgtgt gcagctgaat
     4081 ctatgttgga agtaatgccg atgaccggtt tcaattctca atcccccttc ttttcatgat
     4141 gaatgaaagt ctccttgcga acccacctcc atgacccatc agaataatct gacgaataag
     4201 ttgttataac cttatcaaac ctgaaagaac ctttcaagtc gtttttcaac tattttattt
     4261 attttgtata gataaaatag actgtttgac ggaaaatatc aataagttca attatatggt
     4321 tgaaataagt tgtttcaagt cttatactta tttgaaaata aagagaacgg gagtgttgca
     4381 gaatgtccga ggtgcttgtg acggtcgaga ggggccaatt ggttgaaagt ttacatagag
     4441 gtgacattgc aattgtcagc gcaaacggcg atgtgatctc ctctgtcggt gatatcgaaa
     4501 aggttgtatt tgcacgctca tccatgaaac cgttgcagac cattccgatt atcgaaacg
//
LOCUS       DASULI010000334         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_262773, whole
            genome shotgun sequence.
ACCESSION   DASULI010000334 DASULI010000000
VERSION     DASULI010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262773"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VFX34_07145"
     CDS             <1..1605
                     /locus_tag="VFX34_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX5564720.1"
                     /translation="ETFQSILKNDEQKTTLKIGSSTFQVIYRAEEKLIYLFDITEKVK
                     IESLYHQDRTVLGVMLIDNYDELAQTMDDQTRSNLNSLVTSLVNQWALDNGIFVKRIA
                     SDRFLAVFNEAILSKLETAKFTILDDIREKTAKQNAGLTLSIGVGTGSAALTELGELA
                     QSSLDLVLGRGGDQVAIKHKDGKLKFFGGKTNPVEKRTRVRARVISHALRDLIQGSDK
                     VLIMGHRMPDMDAIGASIGVRKMARMNNIEGHVVLNFDELDGSVTRLMDEIKKDEELF
                     SQFVDPETALSMLTEKSLLVIVDTHKPSMVIDERLIDKAEKVVVIDHHRRGEEFINNT
                     MLVYMEPYASSTAELVTELVEYQPKNEKLTMLESTALLAGIVVDTKSFTLRTGSRTFE
                     AASYLRTNGADTVLVQRLLKEDIETYIQRSKLIETVELTPEGIAIARGEEGVVYSPVL
                     IAQTADILLTMQGVIASFVLASRSDGKIGISARSLGELNVQLVMEELGGGGHLTNAAC
                     QIEAETIEEAVHLLKTAIEDKSERGK"
     gene            1608..2054
                     /gene="rplI"
                     /locus_tag="VFX34_07150"
     CDS             1608..2054
                     /gene="rplI"
                     /locus_tag="VFX34_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5564721.1"
                     /translation="MKVIFLKDVKGKGKKGEVKEVSVGYAQNFLLKNKLAIEATPANL
                     SQLEGQKKRAEKDAAAELAEAKKLKEVIDETTVEMKAKSGEGGRLFGSITTKQIADAL
                     EKNHKIKVDRRKMELPDAIRALGYTNVPIKLHPEVTATLKVHVTEE"
     gene            2067..3425
                     /gene="dnaB"
                     /locus_tag="VFX34_07155"
     CDS             2067..3425
                     /gene="dnaB"
                     /locus_tag="VFX34_07155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5564722.1"
                     /translation="MMDQMYDRVPPHNNEAEQSVIGAIFLQPEALITASEILRPDDFY
                     RTAHEKIFDTMQILSDRGQPIDVVTVTEELSAKKELEDVGGISYLTEIANAVPTAANI
                     EYYAKIVEEKSLLRRLIRVATNIVEEGFTKEDEVEAVLSEAEKRIMEVSSRKNAGDFK
                     HIKDVLVETYDNIEILHTRKGEVTGIPTGFRDLDKITAGFQRNDLIIVAARPSVGKTA
                     FALNVAQNVATKAGENVAIFSLEMGAEQLVMRMLCAEGNIDAQVMRTGNLEAEDWRKL
                     TMAMGSLSNAGIFIDDSPGIRINEIRSKCRRLQQEHGLGMIMIDYLQLIMGSGRSGEN
                     RQQEVSEISRSLKALARELKIPVIALSQLSRGVEQRQDKRPMMSDLRESGSIEQDADI
                     VSFLYREDYYDKETENQNMIEIIIAKQRNGPTGTVTLAFAKEYNKFLNIDWSQHQTPA
                     YQ"
BASE COUNT         1107 a          811 c          972 g          832 t
ORIGIN      
        1 gaaacgtttc agtccattct taaaaacgat gagcagaaga caactttgaa aatcggtagt
       61 tccacttttc aagtcatcta tcgggctgag gaaaaactga tctatttatt cgacatcacc
      121 gaaaaagtga aaatcgaatc cctatatcac caagaccgca ccgtgctcgg ggtcatgctt
      181 atcgacaatt acgatgaact tgcccagacg atggacgatc agacgagaag taacctcaat
      241 tcgcttgtca cttcactcgt gaatcagtgg gcgctggata acgggatttt tgtgaaaagg
      301 attgcgtccg accgtttttt agcagttttc aatgaagcga tcctgtccaa gttggaaacg
      361 gcgaaattta cgatattgga tgatattcgt gagaagaccg ccaagcagaa tgcgggcctg
      421 acactgagca ttggagtggg aacgggctcc gccgcactga cagagcttgg tgagctggca
      481 caatccagtc tggatcttgt tctcggacgc ggaggcgatc aggtcgccat caagcataag
      541 gatggaaagc tcaaattttt cggcggcaag acgaatccgg tggaaaaaag gacccgagta
      601 cgcgccaggg tcatttctca cgcattgcgg gatttgatcc aaggcagcga taaagtgctg
      661 atcatgggcc ataggatgcc cgatatggat gcaatcgggg cttccatcgg tgttcggaaa
      721 atggcgcgga tgaacaacat tgaaggccat gttgtcctca actttgacga gctggatgga
      781 agcgtgaccc gcctgatgga tgaaatcaaa aaggacgaag agttgttttc gcaatttgtc
      841 gacccggaaa ccgcattgtc gatgctgacc gaaaaatccc ttcttgtcat tgtcgatacg
      901 cataaaccga gcatggtcat agacgagcgg ctgatcgata aggccgaaaa ggtcgtcgtc
      961 atcgaccatc atcgacgcgg cgaggaattc atcaataata cgatgctcgt ctatatggaa
     1021 ccatacgcat cttctactgc tgagctcgtg acggagctgg tggaatatca gccgaaaaat
     1081 gaaaaactga cgatgctcga atcgactgct ttgctcgcag gaatagtcgt cgacacgaaa
     1141 agcttcacat tgcggactgg gtcgaggact tttgaggcag catcctattt acggacgaac
     1201 ggagccgaca cggtactcgt acagcggctg ttgaaggaag acatcgagac gtacattcaa
     1261 aggtccaagt tgattgaaac ggtcgagctc acccctgagg gtattgcgat agcaagaggg
     1321 gaggaaggcg tcgtctatag cccggtgctc attgcgcaga ctgcggatat cctcctgacg
     1381 atgcaagggg tcatcgcctc gttcgtccta gcttcccgtt cagatgggaa aatcggcatc
     1441 agtgcaagat cccttggtga gctaaatgta cagctcgtca tggaagaact tggcggcggc
     1501 ggacatttga cgaatgctgc ttgccagatt gaagcggaaa cgatagaaga agcggttcat
     1561 ttattgaaaa ccgccataga agataaatcg gaaaggggaa aatgacgatg aaagttattt
     1621 ttttgaaaga cgtcaaaggc aaaggaaaaa aaggtgaagt gaaagaagta tcagtcgggt
     1681 acgcgcaaaa cttcctgttg aaaaacaagt tggcgattga agcgactcct gcaaatctca
     1741 gccagcttga aggccagaag aagcgcgcag aaaaagatgc agcggccgaa ttagccgaag
     1801 caaaaaaact gaaggaagtc atcgatgaga cgactgtaga aatgaaagcg aaatccggag
     1861 aaggcggacg tctcttcggt tcaattacaa cgaagcagat tgcagatgct cttgagaaaa
     1921 atcataaaat caaagtggat cgccgtaaga tggaactgcc tgatgcaata cgggcactag
     1981 ggtatacaaa tgtaccgatt aaactgcacc cggaagttac cgctacgctt aaagtccatg
     2041 taaccgaaga atgataggga gcgataatga tggatcagat gtatgatcgc gtccctccgc
     2101 ataataacga agccgaacaa tctgtcattg gagcgatttt cttacagcct gaagcattaa
     2161 tcactgcttc agagattctt cgtccagacg acttttacag aacagcccat gaaaaaatat
     2221 tcgacacgat gcaaatcctc agcgataggg gacaaccgat tgacgttgtt acggtgacag
     2281 aagaactttc cgcgaagaag gaattggaag acgtcggtgg catttcatat ttgaccgaaa
     2341 ttgcaaatgc cgtcccgaca gcagcgaata tcgagtatta tgccaagata gtcgaagaga
     2401 agtcgcttct gagacgactc atccgcgtag caaccaatat tgtcgaggaa ggttttacga
     2461 aagaggatga agtcgaagcg gtcctttccg aagcagaaaa aaggatcatg gaagtatcca
     2521 gtaggaaaaa tgccggtgat ttcaagcaca ttaaagatgt acttgtggaa acatatgaca
     2581 atattgagat cctccatacg agaaaaggag aagtgacagg tattccaact ggattccgtg
     2641 acctcgacaa aattacggcc ggttttcagc gaaatgacct aatcatcgta gctgcccgtc
     2701 cttccgtcgg taaaacggcg ttcgccttga acgttgcgca aaacgtcgcg acgaaggcgg
     2761 gtgaaaacgt tgcgattttc agtttggaga tgggcgcgga gcaactggtc atgcggatgc
     2821 tttgtgcgga agggaatatc gacgcgcaag tgatgcggac aggaaatttg gaagccgagg
     2881 attggcggaa attgacgatg gcgatgggga gtctatcaaa tgccggtatc ttcattgatg
     2941 actctccagg catccggatt aatgaaatcc gttccaaatg tcgccgtctc caacaggaac
     3001 atggtctggg catgatcatg atcgattatt tgcagctcat catgggaagc ggccgttcag
     3061 gcgagaaccg tcaacaggaa gtatccgaaa tttcccgttc cctaaaagca ctggcgcgtg
     3121 aattgaaaat tccagtcatc gcgctctccc agctttcccg tggcgtagaa caacgtcagg
     3181 acaagcggcc gatgatgtcc gatttgcggg aatccggaag tattgagcaa gacgcggata
     3241 tcgtttcttt cctttatcgt gaagattact atgataaaga aacagagaat cagaatatga
     3301 ttgaaatcat catagcgaag cagcggaacg gcccaaccgg caccgtcact ctagctttcg
     3361 caaaggaata caacaagttt ttgaacatcg actggagcca acaccaaacc ccagcctatc
     3421 aataaattaa taaagaagcg tatgggattt gcattttccc atacgcttct tttttgattt
     3481 aacttctttg ttttataaat tgaattacta ggattgcagc gaaaggcggc gactcctggg
     3541 ggattagcga acgccgttac gaagaacggc gtttgcgagc aaaagcgaag cgttgtaagc
     3601 aggatggaca gaacagatgc cttaatttct gcaaaaagca cagaaatacg gcaaatcgga
     3661 ctcttcgttg tccgattggc tcaacgcccg ccccccggaa agcgtccgcc tgaagcgaaa
     3721 at
//
LOCUS       DASULI010000335         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_924398, whole
            genome shotgun sequence.
ACCESSION   DASULI010000335 DASULI010000000
VERSION     DASULI010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924398"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1301
                     /locus_tag="VFX34_07160"
     CDS             66..1301
                     /locus_tag="VFX34_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018661610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX5564723.1"
                     /translation="MQIPVDKGLDHTFDLLTEGYHFMRNRFDELGTDIFETRLMGKKM
                     ICMKGPEAVELMYDSSKFQRSGAVPKRIQKSLFGEGGVQTLDGNAHKVRKLMFLSLMT
                     EASIKRVHQLTLEQWRLRASQWKSGQEVVLFDEAEEVLCRVACLWTGVPLKESEVSKR
                     AYDLGALIDAFGGVGPRYREGRNARKRTEDWIRSLIEKFRSGAVDGVDHALEVIATHR
                     DEKGQLLDAQIAAVELISVIRPIVAVARFIVFESLALYDYPQYRESLKEADDDFVEAF
                     AQEVRRYYPFTPFLGAVVRHDFEWKGYQFMKGDTVLVDIYGTNHDRESWNEPDLFKPE
                     RFLEEKVTLYNFVPQGGGDPARGHRCPGEEITVAIMKASLSFLVNELDYTVVQGQDLS
                     INFTRMPTLPASRMILKLN"
     gene            complement(1510..2163)
                     /locus_tag="VFX34_07165"
     CDS             complement(1510..2163)
                     /locus_tag="VFX34_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564724.1"
                     /translation="MKHLRNERGNGMVYMLWIMVMSIAICVIVLNFAKVYAVKQQAAT
                     ATQQAALAGTSVLVNAAKEGFISFDMLIDDEDVVKQLLDDGGKSIEDLVDEKAQEYRY
                     QGLPKDIAYINAMNDILTPRMETHGSLKSLVNDQVILANGDFKQTVREVVKKNGGNEE
                     RIEVVFTSDFRVEVKADVTYESITDSAEKYIEKITKEVPQKGYGPSLLFLRGVSTDP"
     gene            complement(2160..2564)
                     /locus_tag="VFX34_07170"
     CDS             complement(2160..2564)
                     /locus_tag="VFX34_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX5564725.1"
                     /translation="MLTLKKFKSIRNERGSMSIEFIGILPFYFLFFLLLWQVVASGYA
                     VLSLKSAANDGAQVYALTGSYSEAVDAVNESIGDSSLITNPLVTVTSGGGDGLFDFKI
                     SARHPLVFIPEKWKSTMSVAIDSKAAGKVMLR"
     gene            complement(2585..3193)
                     /locus_tag="VFX34_07175"
     CDS             complement(2585..3193)
                     /locus_tag="VFX34_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564726.1"
                     /translation="MTLRQIINEPVKADNLIVFTFFLHLLFLTGASAVPEGRFLFWMP
                     INLTVEAFIMLRLILFWRRFSKSEPRRYNSLQMYWMLMGFSIFSILPFVRMSYGTVAF
                     WPLLIGTLLLFLLGHLLKEKVGEVFVNPRKIKQLVMWPTALAGIVLIGITIMAVLRFQ
                     SVDENVGIAVFLYMLGVFSIFVANPLSLSKERIEKLIGDEEA"
     gene            complement(3251..3658)
                     /locus_tag="VFX34_07180"
     CDS             complement(3251..3658)
                     /locus_tag="VFX34_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564727.1"
                     /translation="MDELTFKKTNTALAVLLTNVTFGVYLPYWFISRRESISQLGKVQ
                     LHYNWLKFLFVMYAFLAFYFVIGGAFLTEMGIDFMDTFNIIITFIGLGFTYYSVFRVA
                     EAIEEKAGAEIFNRVLLILFHIWYLQYKINRYE"
     gene            complement(3646..4314)
                     /locus_tag="VFX34_07185"
     CDS             complement(3646..4314)
                     /locus_tag="VFX34_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564728.1"
                     /translation="MREAAQYYNEQLLKIVAYGLCVMVPAWLFFSNYSLLLLTVDWGG
                     RENLINILLNILMFVIFVRPLFSLYKRVSVSDELEFKEMVKEFIWSIGPLFAIGLFFS
                     ALVYGGIFAFYVPGILIAPILFILPFMYSGTRSIRTWIKSSIDFYINHFVQVWIQIIF
                     WSSIVTLVWAGVLYITSFWEMSYEAYNIARLVFSLVLFPFIVFSLSEKFLSMTEEVED
                     EWTN"
     gene            complement(4311..4667)
                     /locus_tag="VFX34_07190"
     CDS             complement(4311..4667)
                     /locus_tag="VFX34_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564729.1"
                     /translation="MGKMKYVQVLGIVTIAMIVFSFVMVFVIPDFFIHSIIVMLFSYG
                     LLGYLTRSFHFPYFSAYMLACVLVVINMLISHFFVGLPMLFEPDIALFSFVNATAIAI
                     ASVFIFRKVDARRAYG"
BASE COUNT         1418 a         1091 c         1032 g         1219 t
ORIGIN      
        1 gttagatgct tctcaaactg ggaatattca cagtaacgca atgtgaagag gagttggagg
       61 attacatgca aataccggtg gacaaaggtc ttgatcatac gtttgatttg ttgacggaag
      121 ggtaccattt catgcggaat cggttcgacg aattaggaac tgacattttt gagacaagat
      181 tgatggggaa aaagatgatt tgcatgaaag ggccggaagc tgtggagtta atgtatgact
      241 cttcaaagtt ccaacggtct ggtgcggttc cgaagcggat tcagaagtcg ctgttcggag
      301 aaggcggcgt gcagacgctc gatgggaatg cccataaagt gcgcaagttg atgtttttat
      361 cgctcatgac agaagcgtcg attaaacggg tccatcaatt gacattggag caatggagac
      421 ttcgggcaag ccaatggaaa agcggacagg aagtcgtcct gttcgatgaa gccgaggaag
      481 tcctatgtcg ggtcgcttgt ttatggactg gggtgccgtt aaaggaatcg gaagtgtcaa
      541 agcgggctta tgacttggga gcattgatcg atgcgttcgg aggagtcggc cctagatatc
      601 gtgaaggccg gaatgcgcgg aagcggaccg aggattggat ccgttcactg atcgagaaat
      661 tccgttcggg agcggttgat ggcgtcgatc atgcgctcga agtcatcgca acgcaccgtg
      721 atgagaaagg acagttgttg gatgcgcaaa tcgcagcggt cgaattgatc agcgtcatcc
      781 gtccgattgt ggcggtcgcc cgattcatcg tatttgaatc actcgctcta tatgattatc
      841 cacaataccg tgagtcgttg aaggaagcgg atgatgattt tgtagaggcg ttcgcccagg
      901 aagtccgccg ctattaccct ttcacccctt tcctcggtgc agtcgtccgc cacgattttg
      961 aatggaaagg ttatcaattc atgaaaggcg atactgtgct agtcgacatc tatggcacaa
     1021 accatgaccg ggagagttgg aatgagcctg atttgttcaa gccggaaaga tttctggaag
     1081 agaaagtgac cttatacaat tttgtaccgc aaggcggagg agatcctgca cggggacacc
     1141 gctgtccagg cgaggagatc actgtcgcga tcatgaaagc gagtctttcc ttccttgtga
     1201 atgaacttga ctataccgtc gtccagggtc aggatttatc aatcaatttc acccgtatgc
     1261 cgaccctgcc ggcaagccgg atgatactta aattgaattg aaaacttgat gagacggcaa
     1321 ccgcccgaat aggcatgaag accgcctgat aacatggctg aaccgcctaa agtggtatcg
     1381 aaaccgcctg aagtggtttt gaaaccgcct gatatcttga tctaaccgcc taaagcagca
     1441 tgaagaccgc ctgataactt taacaaacat aaaaaagatg aagggaaatt gtattttcct
     1501 tcatcttttt tacgggtcgg tagacacacc tcttaaaaat agcaaggatg gtccgtagcc
     1561 tttttgagga acttctttcg ttatcttttc aatgtatttc tcagcactat ccgttatcga
     1621 ttcatacgtg acgtccgctt tcacttcgac acggaaatca gaagtgaata cgacttcaat
     1681 ccgttcctcg ttgcctccgt ttttcttaac tacttctcgc acggtctgct tgaaatcacc
     1741 attagcaaga ataacttgat cgttcacaag ggattttaat gaaccatgag tctccatcct
     1801 tggcgtcagg atgtcattca tcgcattgat atacgcaata tcttttggta acccttgata
     1861 ccgatactct tgagcctttt cgtcaaccag gtcctctatg gatttgccgc cgtcgtccaa
     1921 caattgtttc accacatcct catcatcaat aagcatgtcg aatgatatga atccttcctt
     1981 agccgcattg accaacacgg acgttcctgc gagtgcagcc tgttgcgttg cggttgcagc
     2041 ctgttgcttc acggcgtaca ctttcgcgaa attcaagacg atgacgcaga tggcgattga
     2101 catgaccatg atccaaagca tatacaccat gccgtttcca cgttcgttcc ttagatgctt
     2161 catcgtagca tcaccttgcc ggctgctttc gagtcgatgg caacagacat cgtcgacttc
     2221 cacttttcgg gaatgaagac gagcggatgc ctagctgaaa tcttaaagtc gaagagcccg
     2281 tccccaccgc ctgatgtaac ggtgacgagc ggattcgtaa tcaagctgct atcgccgatc
     2341 gattcattca ccgcatcgac cgcttcgcta taacttcccg tcaaagcata cacttgcgcg
     2401 ccgtcgttcg ctgcggattt caggctgagc acagcatagc cggaagcgac aacttgccat
     2461 agcaatagga aaaacaggaa gtaaaaaggg agtatgccta tgaactcgat gctcatcgat
     2521 cctctttcat tgcggatgga tttgaacttt ttcagcgtta acaccccttc catcaatctt
     2581 tatatcaagc ttcttcatcg cctatcagct tctctatccg ttctttcgac aacgacaatg
     2641 gatttgctac aaatattgaa aacacgccaa gcatgtataa aaagacagct attccaacat
     2701 tctcatctac cgattgaaac cgtagaactg ccattatcgt aatgccaata aggacaatgc
     2761 cagccaacgc cgtaggccac atgacaagct gttttatctt acgcggattc acaaagactt
     2821 ctccaacttt ctccttcaaa agatgtccta ataggaaaag aagtaaagta ccaattaaaa
     2881 gcggccaaaa agccactgtt ccataactca ttctcacaaa aggcaatatc gaaaaaatag
     2941 aaaagcccat aagcatccaa tacatttgta gagaattata ccgtctcggt tcgctcttgc
     3001 taaaacgcct ccaaaatagg atgaggcgga gcatgatgaa agcttccact gtcagattga
     3061 tgggcatcca aaagaggaaa cgtccttccg gtactgcgct ggcgcctgtt agaaacaata
     3121 ggtgcagaaa aaatgtaaag acaataaggt tatccgcttt cactggctcg ttgattattt
     3181 gtcgtaaagt catcggcttc cctccacttt gtagacaaat gtcattactt cccaccattc
     3241 aacccttcga tcattcatat ctattgattt tatattgcaa ataccagata tggaataaaa
     3301 tcaacagcac ccggttgaaa atttccgctc cagccttctc ttcaatcgcc tctgcgacac
     3361 ggaacacaga ataatatgta aagccaagcc cgatgaatgt gatgataata ttgaatgtat
     3421 ccataaaatc tatccccatc tcggtcagga atgcgcctcc gattacaaaa tagaatgcga
     3481 gaaacgcata catgacgaac aggaatttca accagttgta atgcagttgc actttgccaa
     3541 gttggctgat tgattctctc cgtgaaatga accaatacgg caagtaaacg ccaaatgtca
     3601 cattcgtcag caaaacagcg agagcggtat tggtcttttt aaatgtcagt tcgtccattc
     3661 atcttccacc tcttctgtca tgcttagaaa tttttcggat aaggagaata ctataaaagg
     3721 gaaaagcact aatgagaata ccaatcgtgc tatgttgtac gcctcgtagc tcatttccca
     3781 aaaagatgtg atgtacagca cgcctgccca cactaacgtc acgatggacg accaaaagat
     3841 gatttgtatc caaacttgca cgaaatgatt aatgtagaaa tcgatggagg atttaatcca
     3901 cgtgcgtatc gatcttgttc cgctatacat gaaaggtaaa atgaataata tcggtgcaat
     3961 cagtattcct ggaacataga atgcgaaaat ccctccatac actaaagcgg aaaagaacaa
     4021 gccgattgca aatagaggcc cgattgacca aatgaattcc ttgaccattt ccttgaattc
     4081 caattcatcg cttacactga ctcttttgta caaggaaaat aacgggcgga cgaaaatgac
     4141 gaacatgagg atattcaata aaatattgat aagattttcc ctgcctcccc aatcgacagt
     4201 caatagaagt aaactataat tggaaaagaa gagccaggca ggaaccatta cacaaagccc
     4261 atatgcgacg attttcaaca actgttcatt ataatattga gccgcttccc tcatccatat
     4321 gccctccttg cgtcaacttt cctgaaaatg aacacacttg caatcgcaat tgcggttgca
     4381 ttaacaaaac taaagagtgc aatatccggt tcaaatagca ttggaagccc gacaaagaag
     4441 tgggaaatca acatattgat gacgaccaaa acgcatgcca gcatataggc ggagaagtat
     4501 ggaaaatgaa atgacctggt tagatagcct aacaatccat aagagaatag catgacgatg
     4561 atggaatgaa taaaaaaatc cgggatgacg aagaccatga caaaactgaa tacaatcatc
     4621 gctatggtta cgattcccaa cacttgtaca tacttcattt ttcccatctc ccctccttca
     4681 caaaggattt atacgccagc gaactgaaca agaacagtag ccaacaagca aatatatata
     4741 aaattattga aatataagtc
//
LOCUS       DASULI010000336         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_781080, whole
            genome shotgun sequence.
ACCESSION   DASULI010000336 DASULI010000000
VERSION     DASULI010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781080"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFX34_07195"
     CDS             <1..375
                     /locus_tag="VFX34_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5564730.1"
                     /translation="YMLKEMDADAIVQAIKVVAAGGSYLHPKVTHNLVSEFRRLSERE
                     HKGSFQQNDIRRPLHLLTKRECEVLQLLTDGQSNRTIGETLFISEKTVKNHVSSILQK
                     MGVNDRTQAVVTAIKNGWVEVK"
     gene            437..1009
                     /locus_tag="VFX34_07200"
     CDS             437..1009
                     /locus_tag="VFX34_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5564731.1"
                     /translation="MKKVFVFSMLIFVLILGACSKKDDASDHGSVNDGEAPSEFGSID
                     HGVDENKVGFNLTGGTIEEAANVPAEEKELIIQSFDTYIHAFNNQDIDEYMSTLSEKP
                     KSFKLDEEREYIESVFEQYELSREAKDITIVKFDEESGEAQVFANLVTKLKQKSTGLE
                     TNRDGRQVTVFSKEGGDWKVNSVYYMEDQE"
     gene            1299..1673
                     /locus_tag="VFX34_07205"
     CDS             1299..1673
                     /locus_tag="VFX34_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564732.1"
                     /translation="MPLIPAKALPHFENMIYLPMVLIVLQRDRELFEKGPFKLKGPYL
                     KIVDNTMKQVQSELQETTFYLRRNNMKVIRKTRDSMFTEYIFINGGYEDHRRYLNVRL
                     RNRTEELLDLYFTRSSNQLMGH"
     gene            complement(1639..2172)
                     /locus_tag="VFX34_07210"
     CDS             complement(1639..2172)
                     /locus_tag="VFX34_07210"
                     /inference="COORDINATES: protein motif:HMM:NF018087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HEX5564733.1"
                     /translation="MTEFEPRYYLSFDTLMLQPKKEGNKIFTRVIERNRIVAVPKKSI
                     HIITGSCTFYGSSYTTVMNTSRRLFGDNKQKAPFLLANAFGIPFIFIPTLSPLSDQNV
                     WISYHAIDFYAEDGLGTSVALENGEKYYLDISVTTMNRQYAFAKLLERDFLKRQRSFH
                     SSLRFPPNGPSTDYSTL"
BASE COUNT          727 a          528 c          597 g          601 t
ORIGIN      
        1 tatatgctga aggaaatgga tgcggacgcg attgtacaag caatcaaagt cgttgcggca
       61 ggcggatctt atttgcatcc gaaagtgacg cataatcttg tctccgaatt ccgccgtctg
      121 agcgaacgtg aacataaggg atcgttccag caaaatgaca tccgccgtcc gcttcatcta
      181 ttgacgaagc gcgaatgcga agttttacag ctgctgacgg atggacaaag caaccggacg
      241 atcggtgaaa cgcttttcat ttctgaaaag acggttaaaa accatgtttc gagcattttg
      301 cagaaaatgg gggtcaatga ccgtacacaa gcggttgtca cagcaatcaa aaacggctgg
      361 gttgaagtga agtgatggta tactgaaagg ggcctcctac taggaaggtc ccttttaccg
      421 tacaggagga aaaataatga aaaaagtgtt tgttttctca atgctcatct tcgtgctcat
      481 ccttggcgca tgttcgaaaa aggatgatgc atcggatcac ggttcagtga atgacgggga
      541 agccccgagc gaattcgggt cgatcgatca cggcgtggat gaaaataaag taggcttcaa
      601 tttgactggc ggcacgatcg aagaggctgc aaacgtgccg gcggaagaga aagagctgat
      661 catccaatcg ttcgacacgt atatccatgc gttcaacaac caggacatcg acgagtatat
      721 gagcacactt tccgagaagc caaagagctt taaattggat gaggaacgtg agtatatcga
      781 aagcgtattt gaacaatatg aattgtcccg tgaggcaaag gatatcacaa tcgtgaaatt
      841 tgacgaagag tcgggtgaag cccaagtgtt tgccaacttg gtgacgaagt tgaaacagaa
      901 gtcaactgga ttggagacga accgcgacgg caggcaagtg accgtctttt caaaagaggg
      961 cggcgattgg aaagtgaact ccgtctatta tatggaagac caggaataac gtttatttac
     1021 aatgaaatcc ccacagacta gaactgtcca agcaaatttt gctgggcagt tttattatgc
     1081 aaaagcatag gtcgcctgcc tagatgcgac aggcataagg cgaagatgcg aagtggcggt
     1141 ttttgccaca cagcagcttt aacttatgac cccgagcgtc tggcgaccga tgctggatga
     1201 tatttgaata caaaaattac cttcgccgcc atattgcttt ccggaaatga aaaaggtata
     1261 atgggaacaa atgttccatc tgaagggagg aaaccgtgat gcctttaatt ccggcgaagg
     1321 cattgcctca tttcgaaaac atgatctatt tgccgatggt cctgatcgtg ctccaacgcg
     1381 accgggagct atttgaaaaa ggcccgttca aattgaaggg cccttacttg aagattgtcg
     1441 acaatacgat gaagcaagtc caaagcgagc tccaggaaac gacattctat ctgcggcgca
     1501 ataatatgaa agtgatccgg aaaacgaggg atagcatgtt cacggaatac atcttcatca
     1561 atggcggcta tgaagaccac cgccgctatt tgaatgtccg gctccgcaac cggacggaag
     1621 aattattgga cctgtatttt acaaggtcga gtaatcagtt gatgggccat taggcgggaa
     1681 ccgtaaagaa gaatggaagc tcctctgcct tttcaggaaa tccctttcga gcaatttggc
     1741 aaaagcgtat tgccggttca tcgtcgtaac cgaaatatcc aagtagtact tttccccatt
     1801 ctccaatgcg acggaagtcc cgaggccatc ttccgcgtaa aaatcgattg catggtagga
     1861 aatccataca ttctgatccg atagaggaga caatgtcgga atgaagatga agggaatgcc
     1921 gaaagcgttt gccaacagaa aaggcgcctt ctgcttatta tccccaaata atcgcctgga
     1981 tgtattcatg acagttgtat aactggaacc atagaaagtg caggaacccg ttataatatg
     2041 tatcgacttt tttggaacgg ccacaatcct gttccgttca atgacccttg tgaaaatctt
     2101 attgccttct ttcttcggtt gcagcatcag ggtatcaaac gataaataat accttggttc
     2161 gaattcagtc aaatccactt ccccctatct tattaatttc ctacacccct ctctagtatt
     2221 ataggacaag gaattagaaa aacaagcctt ttgaatgaat aatttgaact attcgataaa
     2281 aatgcaatcc tactttccgt ccataaaaaa atcccccgtc cctgaacggg ggaagtgaag
     2341 tttattcagc ttgataatcc aatgcggcag cccccagtgt tttcgcggcg agcagcatcg
     2401 cctgttcctc gaaatcgaat ttcggatggt gatgcgggta ggtgtcagat ggc
//
LOCUS       DASULI010000337         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1780445,
            whole genome shotgun sequence.
ACCESSION   DASULI010000337 DASULI010000000
VERSION     DASULI010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780445"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFX34_07215"
     CDS             <1..395
                     /locus_tag="VFX34_07215"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358310.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX5564734.1"
                     /translation="YMDEDGYFFFQGRVDDVIMTSGERVGPFEVESKLLEHPAIVEAG
                     VIGKPDPVRGEIIKAFVALHEGYEPSEELEDDIKQFVKKELAAHAAPREIEFKDKLPK
                     TRSGKIMRRVLKAWELNLPTGDLSTMED"
     gene            complement(445..>3306)
                     /locus_tag="VFX34_07220"
     CDS             complement(445..>3306)
                     /locus_tag="VFX34_07220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5564735.1"
                     /translation="IFGLTLGVFAASVGAGYFASLVAKEPLRSKAEMRGDVFNYEETS
                     EIYFANDIYLGKVNADIERKETTLKDVSPFVIDAVLATEDEYFETHDGIVPKAIFRGL
                     FQDVTNSSSQTGGSTLTQQLIKNQILTNEVSYERKAKEILLAMRLEHFMEKEEILEAY
                     LNIIPYGRNAMGQNIAGIETAAEGIFGVSAKELNLPQAAYIAGIPQAPFAYTPFLSNY
                     YGGGLKKDENIKPGIDRMKTVLFRMKETGYISDKQYEEAIAYDVTKDFIEKTERATER
                     YPYLTQEIQERTKDILAEILAEKDGIDADRLKEEKKLIEKYRILAERDMRTGGYRIYS
                     TVDKELYDGMNKVAEEFENYGITYTREEQDIVTGETILKDDPVQVGAIMIENSTGRIL
                     SFIGGRDHNLENTNHATRAFRQNGSSMKPLLVYAPAIEYGLIGAGSPVVDVKFEIPDG
                     RGTWSPSNYNANVENGIIPARQALAESLNLPTARLYNEIVDRRPAEFLEKMNFTKLTA
                     GDYVHLSVSYGGMDVGVSVEENTNAFATFANGGKFIQSYIIDRIEDMSGNVVYEHKVE
                     PVDVFSPETSYIITDMLRDVIKPGGTGQQLPGFMNFKTDLAAKTGTTDKYGDAWLVGY
                     NPNVSMGLWFGYKDQKRKLYTRKSGAMHPTTRTTMLFGRLMNKANEVRPDIIGAGSTF
                     KQPEGVVSKAFCGISGLAPSAACTAAGLVRSDLFNSKVMLPTKPDDSIINASYVSVKG
                     SRYMALPSTPAEFIVSGGTGVNQEFIDRMLGPWGGDASKLFPSISKFASSVVSGAVFP
                     ADDVAPAGVSASLNGSTLVWSESPSNDVIGYYVYRNNERIATIVDGSANSLAVGPGNY
                     HVRAVDITGLLSGSSNIVTIDSPEPPPTEKPDDEKTPPATPPGNDDNDGETGSGNNGG
                     NNGGDNGNNGNGNDGNNGNNGNGNNGGGNKPPSDDDDDD"
BASE COUNT          797 a          820 c          754 g          935 t
ORIGIN      
        1 cctatatgga cgaagacggc tatttcttct tccaaggaag ggtcgatgat gtcatcatga
       61 cgagcggtga acgggtaggc ccgtttgaag tggaaagcaa gttgcttgag cacccggcga
      121 ttgtcgaggc aggggtcatc ggaaaacccg acccggtacg cggcgagatc atcaaagcct
      181 ttgtcgcact ccatgaagga tatgaacctt ctgaagaact ggaagatgac attaagcaat
      241 ttgtcaagaa ggaacttgcc gcacacgctg caccaaggga aatcgaattc aaggacaagc
      301 ttccgaagac gagaagcgga aaaatcatga gacgtgtatt gaaggcatgg gaactgaact
      361 tgccgacagg cgacttatca acgatggaag actgatgaaa cgagaaaagg gactcccggt
      421 tgtggagtcc cttttcttat tcaattaatc atcatcatca tcgtcggaag gtggtttatt
      481 gcctcctcca ttgttgccgt ttccgttgtt cccgttattt ccgtcattgc catttccatt
      541 gttgccgtta tccccaccgt tattcccgcc gttatttccg ctgcctgttt cgccatcatt
      601 atcatcattt cctggcggcg tcgcaggagg cgttttttca tcatccggtt tttctgtcgg
      661 cggcggttcc gggctatcaa tagttacaat attggagctc ccggataaga gtccggtaat
      721 atcaacagct ctgacatggt aattacccgg cccgacagcg agcgaattgg cggacccatc
      781 aacgatcgtt gcaatccgtt cattgttccg gtacacataa taaccgatca catcatttga
      841 cggagattcc gaccaaacca atgtcgatcc gttcagagat gcgctgactc ctgcaggggc
      901 tacatcatcg gcagggaaga ccgcaccaga tacgacgcta gacgcgaact tggatatgga
      961 tgggaacagc ttagaagcat ccccgcccca aggtcccagc atcctgtcga tgaattcctg
     1021 gttgactcct gttccacctg aaacgatgaa ttcggcaggc gttgaaggga gcgccatgta
     1081 acggctgcct ttgacggaga cataggaagc attgattatg ctgtcatccg gtttagtagg
     1141 cagcatcact ttggaattga acaggtcgga tctgaccaat ccggctgcag tacatgcagc
     1201 agacggcgcc aatcctgaaa tgccgcagaa cgccttggat actacaccct ctggctgttt
     1261 aaaagtgcta ccagctccga taatatcagg tctcacttca ttcgctttat tcatcaatct
     1321 gccgaaaagc atagtcgttc gggttgtcgg atgcatcgca cctgatttcc gggtatacaa
     1381 cttgcgcttc tgatccttat aaccaaacca gaggcccatc gagacatttg gattataccc
     1441 gactagccaa gcgtctccat atttgtccgt cgtcccggtt tttgctgcca ggtcggtttt
     1501 gaaattcatg aaaccaggca attgttgtcc tgtaccgcca ggctttataa catccctgag
     1561 catatctgtt atgatataag atgtttcagg gctaaacacg tcgacaggtt caaccttatg
     1621 ctcatacacg acattgccgg acatatcttc gattcgatca ataatgtatg actggatgaa
     1681 cttgccgcca tttgcgaaag tggcgaaagc gttagtattt tcctcgacgc tgactccgac
     1741 atccattccg ccgtatgaaa cggaaaggtg gacgtaatct ccagccgtga gtttggtgaa
     1801 gttcatcttc tcaaggaact cagctggccg ccgatctacg atttcattgt aaagccttgc
     1861 tgtcggtaag ttgagggatt cagccaatgc ctgtctcgcg gggataattc cgttttccac
     1921 gttagcatta tagtttgaag gactccatgt acccctgccg tccggtattt caaatttaac
     1981 atcgacgacc gggcttccag cgccgattag gccatattca attgctggtg catagacgag
     2041 cagcggcttc atggaagatc cgttttgcct gaaagccctt gtcgcatgat tcgtgttctc
     2101 aagattatgg tcgcgtcctc cgataaatga gaggattcgg cctgtactat tttcaatcat
     2161 aatggctccg acttgcacag gatcgtcttt cagaatggtt tcgcctgtaa cgatatcttg
     2221 ttcctcacgt gtataagtga ttccataatt ctcgaattcc tctgctactt tgttcattcc
     2281 gtcgtaaagt tctttatcga ctgtcgaata aattctgtat ccgcccgtac gcatgtcgcg
     2341 ctccgccaag attcgatatt tttctataag ttttttttct tctttcaggc gatccgcgtc
     2401 aataccatct ttctcagcga ggatctcggc taaaatatcc ttcgtgcgct cttggatttc
     2461 ctgtgtcaaa tacgggtacc tttcagtcgc ccgttccgtc ttttcaatga aatccttcgt
     2521 aacgtcgtat gcaatcgctt cttcatattg cttatcggaa atatagcccg tttccttcat
     2581 acggaagagg accgttttca tcctgtcaat cccaggttta atattttcat ccttcttcaa
     2641 gccgccgcca taatagttgc tcaggaatgg cgtataagca aacggtgctt gtggaatccc
     2701 tgcgatatag gcagcttgag ggagattgag ttcttttgca ctgacaccga aaataccttc
     2761 ggctgcagtt tcgattcccg caatattttg ccccatagca tttctgccat atggaatgat
     2821 atttaagtag gcttccagaa tttcctcttt ttccatgaaa tgttcaagcc gcatggcgag
     2881 gaggatttct ttcgccttcc gctcataaga tacttcattc gtcaaaatct ggtttttgat
     2941 aagttgttgg gtgagtgttg aaccgcctgt ctgactggat gaattcgtca catcttgaaa
     3001 cagtccgcgg aaaatcgctt ttgggacgat tccgtcatgt gtttcaaaat actcgtcttc
     3061 cgttgccaaa acggcatcga tgacgaaagg agaaacgtct ttcaacgtcg tttctttccg
     3121 ttcgatatcg gcatttactt ttcctaaata aatgtcattg gcaaaataaa tttccgaagt
     3181 ctcttcataa ttgaagacat ctccccgcat ttccgctttg gatcggagcg gttccttcgc
     3241 tacgagcgat gcgaaataac cggcaccgac tgaggcggca aaaacgccaa gcgtcagccc
     3301 gaagat
//
LOCUS       DASULI010000338         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1080273,
            whole genome shotgun sequence.
ACCESSION   DASULI010000338 DASULI010000000
VERSION     DASULI010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080273"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFX34_07225"
     CDS             complement(<1..414)
                     /locus_tag="VFX34_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX5564736.1"
                     /translation="MIIPEKLKRGDEIRIIAPSRSASILSEEGVHAAKGRLENLGFVV
                     SFGKHVFDKDLQSSTSIQQRVDDLHEAFADKSVNGILTVIGGFNSNELLPYIDYELIK
                     ENPKVFCGYSDITAIGTAISTQTGMVTYSGPHFSSF"
     gene            complement(433..888)
                     /locus_tag="VFX34_07230"
     CDS             complement(433..888)
                     /locus_tag="VFX34_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564737.1"
                     /translation="MNIRKLQYDEMPPYDLLLLADPARELVEAYIAEGECYVAEDNNA
                     IIGVFVNVPLTKETVEIKNIAVHEDKQGRGIGKKLLLEAIKIAKSNNYNFIEIGTGNS
                     SIGQLALYQKCGFRISGVIKDFFVDHYDEVIMENGIQCMDMIRLRLEIQ"
     gene            complement(1038..1529)
                     /locus_tag="VFX34_07235"
     CDS             complement(1038..1529)
                     /locus_tag="VFX34_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5564738.1"
                     /translation="MTVTYVDWGGHQLKLTWIQGLMPDQELITSVHAFCFHEGKLLMV
                     NLNDRGWDFPGGHIEAGETAEACVCREVMEEAYVAGDCTLLGAIEVSHEENPLWNESS
                     PYPKVGYQVFYRMDITEFLSFDAGFESSERMLIRPEEAAHYHKGWQKGFDEIMDAALR
                     IRG"
     gene            complement(1539..2255)
                     /locus_tag="VFX34_07240"
     CDS             complement(1539..2255)
                     /locus_tag="VFX34_07240"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX5564739.1"
                     /translation="MKRMKRMKAPFAAKRFVDEYFPHCQAAVLAGSVVRGEETPTSDL
                     DIVIFDNQIEEAFRESRIEYGWPIEVFVHSLTSYKTYFRSDAERARPSLPRMVAEGIV
                     LVDTGILSLIKNEAAALLNEGPAPWSAQTLATKRYMLTDALGDFLGTQNEAEALFIAN
                     ALAEAIHEFVLRTNGQWIGASKWIVRALRQYDEDFAERFVAAFQSFYKTGNREGIIRI
                     TDDVLAPFGGRLFEGYSVGK"
     gene            complement(2278..>2457)
                     /locus_tag="VFX34_07245"
     CDS             complement(2278..>2457)
                     /locus_tag="VFX34_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HEX5564740.1"
                     /translation="HYFTAEIVGGEFGTGQGEEYQESRNRGRYEPMWIPIADLISLDV
                     RPMEIAKRLIERMEI"
BASE COUNT          633 a          667 c          441 g          716 t
ORIGIN      
        1 aaagcttgag aaatggggac cagagtatgt gaccattcca gtttgtgtcg atatcgctgt
       61 tccgattgcc gttatatcac tatatccaca gaaaactttc gggttctctt ttattaattc
      121 ataatcaata tatggcagaa gttcatttga attgaaaccg ccaatgaccg ttaaaatccc
      181 gtttacactt ttatcagcaa atgcctcatg cagatcatcc accctttgct gaatggaagt
      241 ggagctttga aggtctttgt caaagacatg tttgccaaat gaaacgacaa aacctaagtt
      301 ctctaaccgt ccctttgcag cgtgaacacc ttcctcggat agaatgcttg cactgcgact
      361 cggtgcgatg attcgtattt cgtccccccg ttttaacttc tcgggtatta tcatctcgat
      421 tccctctttc aattactgaa tttccagcct aagccgaatc atatccatgc attgaatgcc
      481 gttttccata atgacctcgt cgtaatgatc aacaaagaaa tctttgataa ctcccgaaat
      541 acgaaatcca catttctgat agagcgcaag ctgaccaatg cttgaattcc ccgttccaat
      601 ttcaataaaa ttatagttgt tgctttttgc aatcttgatt gcttctagca acaatttctt
      661 gccaatccca cgaccttgct tgtcttcgtg gactgcgata tttttaatct ccactgtttc
      721 tttcgttaac ggcacgttaa cgaagactcc gattatggca ttgttatcct cagcgacata
      781 gcattcacct tccgctatgt acgcttctac gagttcacgc gctggatcag cgagtaagag
      841 aagatcatat ggcggcattt catcatactg caactttctt atgttcattc aagtcacccg
      901 cctttattta agctagagcg aaggtcccga tataaatttc gatattcaat agataacttt
      961 ctttattcaa tagatatcac ctggtattca atagatattt tcaactatcc aatagataat
     1021 taaagttatt caatagatca tccacgaatg cgaagtgctg catccataat ttcatcaaag
     1081 cccttctgcc agcccttatg atagtgggct gcctcttccg gccgaatcag catccgctcc
     1141 gacgattcaa aacctgcgtc gaatgacaaa aactccgtaa tatccatccg ataaaacacc
     1201 tgatagccca ctttcggata ggggctggat tcattccata acgggttttc ctcatgactc
     1261 acttcaatag cacccaaaag cgtgcaatcg cccgctacgt aagcctcttc catcacttcg
     1321 cgacagacac atgcttccgc cgtttcacca gcctcgatat gccctcccgg aaaatcccat
     1381 ccccggtcat tcaagttgac catgagcagc tttccttcat gaaaacaaaa agcatggacg
     1441 cttgtgatca attcctgatc cggcattaga ccctgtatcc acgtcagctt caattgatgt
     1501 ccaccccaat ccacatacgt aaccgtcact accgcccctc acttccctac agaatatccc
     1561 tcaaatagcc tcccgccaaa tggcgctaac acatcatctg ttattctgat gatcccttcc
     1621 cgattgcccg tcttataaaa tgattgaaat gccgccacaa acctttctgc aaaatcctcg
     1681 tcatattgtc tcagcgcgcg cacgatccac ttggaggcgc cgatccattg cccattcgtc
     1741 cggagtacaa attcatggat cgcttcagcg agtgcgttag cgatgaaaag cgcctccgcc
     1801 tcattttgcg ttccaagaaa atccccaagt gcgtctgtca gcatataccg ctttgtcgcc
     1861 aaggtctgtg ccgaccaagg cgccggccct tcattcaaca gcgccgcggc ttcgttttta
     1921 atgagtgata gaattccggt gtccactaaa acaatccctt ccgcgaccat ccgtggcaat
     1981 gaggggcgtg ctctttcagc atcacttcta aaataggttt tataggatgt taaagaatgt
     2041 acgaatacct ctatcggcca gccgtattcg atacgggatt cacgaaaggc ttcttcgatt
     2101 tgattatcaa aaataacgat atccagatct gatgtaggtg tctcttctcc tctgacgaca
     2161 ctccccgcca ggactgcagc ttggcaatgc gggaagtatt catcgacaaa tcgtttggct
     2221 gcaaatggtg ctttcattct tttcatcctt ttcatccttg gttcctccaa ccacttttca
     2281 gatttccatt ctttcaatca atctcttcgc aatctccatc ggccggacgt ccaacgagat
     2341 aagatcggca atcggtatcc acatcggttc atatcgtccg cggtttcgac tttcttggta
     2401 ttcctcgcct tgtcctgttc caaattcccc accgacaatt tccgcagtga aatagtg
//
LOCUS       DASULI010000339         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1780942,
            whole genome shotgun sequence.
ACCESSION   DASULI010000339 DASULI010000000
VERSION     DASULI010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780942"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="VFX34_07250"
     CDS             complement(<1..1042)
                     /locus_tag="VFX34_07250"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX5564741.1"
                     /translation="MDRNHSVDANSKHEQLEHYRSIDKGKKLTTNQGLKVSEDEHSLK
                     AGYRGPTLMEDFHFREKITHFDHERIPERVVHARGFAAHGEFELYESMKKYTKAAFLQ
                     EPGSKTPVFTRFSTVAGSRGSGETVRDVRGFAVKFYTEEGNYDLVGNNMPVFFIQDAI
                     KFPDLIHAVKPEPHNEMPQAASAHDTFWDFVANNQETAHMIMWHMSDRAIPRSFRMME
                     GFGVHTFRFVNEEGIAHFVKFHWKPKLGVHSLVWDEAQKINGKDPDFHRRDLWEAIEK
                     GDFVEFELGVQLIEEKDEFMFDFDILDPTKIWPEEKVPVKIIGKMTLNRNVDNVFAET
                     EQVAFHPGNVVPG"
     gene            complement(1116..1475)
                     /gene="crcB"
                     /locus_tag="VFX34_07255"
     CDS             complement(1116..1475)
                     /gene="crcB"
                     /locus_tag="VFX34_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX5564742.1"
                     /translation="MIVAEAVVIGLGGFIGALLRFFISARMNKEEGIPVGTLVVNLSG
                     SLLIGLLVGMELPKLWTIFLVSGLMGALTTFSTLIKELIQLWKSGQIKKTILYSIWTF
                     GFGALLAYVGFVIGQYC"
     gene            complement(1472..1849)
                     /gene="crcB"
                     /locus_tag="VFX34_07260"
     CDS             complement(1472..1849)
                     /gene="crcB"
                     /locus_tag="VFX34_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX5564743.1"
                     /translation="MKQALFVGIAGALGAICRVGIGNLFVGLPEFPLSTLTVNLLGTF
                     VLCLLSAGVIQRLTHDQRLQTSITTGFLGSFTTFSTFSLETVSLLQDGQVMLAIVYIA
                     ASVIGGLVIGLFGMKIGRKMVSA"
     gene            complement(1846..>3172)
                     /locus_tag="VFX34_07265"
     CDS             complement(1846..>3172)
                     /locus_tag="VFX34_07265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX5564744.1"
                     /translation="YLLNFKDVYPDAKLLRMEQNYRSSKEIVETAALFITRNKYRYRK
                     EMRTANKEAGPIVMKQFEDPKRLLEYVTYELLDEQNLEEVAVLFRNNSSSTMYVNELH
                     RRGIPFYMKDADDRFFSHWVVEDILNFMRLSFNVERKDVFAKIAMKMNLFISRDMLSK
                     FMNKTTSGNVFEAFIQTVQLKGSQIEKLDTCMDVYARIPDMRPTQIIRMIRYELEYEE
                     ALKSRAAKFGYRIDQLLGILDTLEGIASQVRTMVDFANRWKELEQAVQQAKFNPPANA
                     LTLSTFHSAKGLEFRRVFMIDLVKGIIPSEEDETDPMLLEEARRLFYVGMTRAKERLE
                     LLSYRESNGKSKEDSRFLNEVRGILMKPEENPTEKLKKTDWSILPDPDGISNKSELAV
                     GMHVLHRVFGRGVINGIGEEEIRIQFGEAEKRLSLETILSRRLLKEVEL"
BASE COUNT          793 a          839 c          643 g          897 t
ORIGIN      
        1 tgccaggtac gacattgccc ggatggaacg caacttgctc ggtttctgca aagacattat
       61 cgacattccg gttcaacgtc atctttccga tgattttgac gggcactttc tcttccggcc
      121 atatttttgt cgggtccaaa atatcgaagt cgaacatgaa ttcatctttt tcttcgataa
      181 gttgaacacc taattcaaac tcgacgaaat cccctttttc aatcgcttcc cataagtccc
      241 tccggtggaa gtcgggatct tttccgttga tcttctgcgc ttcatcccaa actagcgagt
      301 gtacaccgag tttcggcttc cagtggaatt tcacgaaatg cgcaattccc tcttcattga
      361 cgaagcggaa cgtatgcacg ccgaagcctt ccatcattct gaagcttcta gggattgccc
      421 ggtcggacat atgccacatg atcatatgcg ccgtttcctg gttattcgca acgaaatccc
      481 aaaatgtgtc atgtgccgaa gcggcttgcg gcatttcatt atgcggctct ggcttgacgg
      541 cgtgaatgag atccgggaat ttgatagcat cctggatgaa gaacacgggc atgttgttcc
      601 cgacaaggtc gtaattccct tcttccgtgt agaatttcac ggcaaacccg cggacatccc
      661 ttaccgtctc acctgaacca cggcttccag ccactgtcga aaaccgtgtg aaaacgggag
      721 tcttcgaacc tggttcctgt aggaaagcgg cttttgtata ttttttcatc gactcataca
      781 actcgaactc cccatgtgcg gcaaatcccc gggcatgaac aacccgctcg ggaatccgct
      841 catggtcgaa atgtgttatc ttctcccgga agtggaagtc ttccattagg gtcggtcccc
      901 ggtatccggc tttcagtgaa tgctcgtcct ccgaaacttt cagcccttga ttcgtcgtca
      961 atttcttccc cttgtcgatt gatcgataat gctccaattg ttcatgtttg ctgttcgcgt
     1021 ctacggaatg gtttctatcc atttcatttc ctccattcgg tttcagtagt ctcctactct
     1081 attcccgtca agagattacc caaacaaaca gcaaattagc aatattggcc gatgacaaaa
     1141 cctacataag ctaatagggc tccgaacccg aatgtccaaa tactatagag aatcgttttt
     1201 tttatttgcc cgctcttcca taactgaatc aattctttaa ttagcgttga gaaagtcgtc
     1261 aaggccccca tcaaacctga cactaggaag attgtccata gcttcggcaa ttccattccg
     1321 acgaggagtc caatcagcaa agagcccgac aagttaacga ccaaagtacc gaccgggatg
     1381 ccttcctcct tattcatcct ggctgaaatg aaaaatctta ggagcgctcc aatgaatccc
     1441 cctagaccga tgacaaccgc ttctgctact atcatgcgct caccatcttt cggccaattt
     1501 tcattccgaa cagtccaata acaagcccgc cgataacact cgcagcaata taaacaatag
     1561 caagcatcac ttgaccgtct tgcaggaggc tgaccgtttc caaactgaac gtggaaaacg
     1621 tcgtaaagga acctaaaaag cctgtggtaa tcgatgtttg aagccgctga tcatgtgtca
     1681 gccgttgaat gacgcctgca cttagaagac aaaggacaaa tgtaccaagc agattgacgg
     1741 tcaatgtcga cagcggaaat tctggcaatc cgacaaatag attccctatc cctactcggc
     1801 aaatcgctcc gagcgctcct gcaattccga caaacagggc ttgtttcata gctcaacctc
     1861 tttcaataga cgccttgata aaatcgtctc aagggacaga cgcttttccg cttccccgaa
     1921 ttgaatccgg atttcctctt ccccgattcc gttgatgacc cctcttccaa atacacggtg
     1981 caagacgtgc atgccgacgg ctaattctga tttattgctg atgccatccg gatccggcaa
     2041 tatcgaccag tcggtttttt tcagtttttc agttgggttt tcttccggct tcatcaaaat
     2101 cccccgcact tcattcaaga atcttgaatc ctctttggac ttcccattcg actctctgta
     2161 ggatagcaat tccaaccgtt cttttgcccg tgtcatcccg acataaaaca atcgtcttgc
     2221 ctcttccagc agcatcggat ccgtttcgtc ctcttcggat gggatgattc cttttaccaa
     2281 gtcgatcatg aacacacgcc tgaattccaa ccctttggca ctatggaaag tagataatgt
     2341 caatgcgttc gcaggaggat tgaatttcgc ctgttgcacg gcctgttcca attccttcca
     2401 tcgatttgca aagtcgacca tcgttcgcac ttgggaagcg atgccttcga gtgtatccag
     2461 gataccgaga agttgatcga tccggtagcc gaattttgcc gctcggcttt tcaacgcttc
     2521 ctcatattcg agttcataac ggatcatccg gatgatctgg gtaggacgca tatcagggat
     2581 tcttgcatag acatccatgc aagtgtccaa tttctcgatt tgactgcctt tcagttgcac
     2641 agtttggatg aatgcttcaa aaacatttcc cgaagtcgtt ttattcatga atttggacag
     2701 catatcccgt gagatgaata agttcatttt catggcgatt tttgcgaaga cgtccttccg
     2761 ttccacattg aagctgagcc gcatgaaatt caaaatatct tcaacaaccc aatgggaaaa
     2821 gaaacggtca tccgcatctt tcatgtaaaa agggattccc cttcgatgga gttcattgac
     2881 atacatcgtg gaagaagagt tatttctgaa taaaacagcg acttcctcca ggttctgctc
     2941 atccaataac tcatacgtga cgtattccag aagccgtttc gggtcttcga actgtttcat
     3001 gacaatcggt cccgcctctt tatttgccgt ccgcatctct tttctatatc tgtacttatt
     3061 gcgtgtaatg aaaagggccg cagtttccac gatctctttt gacgaccggt aattctgctc
     3121 catcctgagc aatttcgcat cagggtaaac atctttgaag ttcagcaaat aa
//
LOCUS       DASULI010000340         4485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1745494,
            whole genome shotgun sequence.
ACCESSION   DASULI010000340 DASULI010000000
VERSION     DASULI010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745494"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFX34_07270"
     CDS             <1..517
                     /locus_tag="VFX34_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009374106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX5564745.1"
                     /translation="VFESGYNPEDAPHLGEVTIATYTTYFKPSDTNRNKNIELSAKSI
                     NNTIVGSGDQFSFNTVVGPRDEASGYQPAPEIINKKLVMGIGGGVCQTSSTVFNAVDQ
                     LQVKYIEWHHHSIDIGYVPKGRDATVSYGGLDFRFQNTAEAPFLIKTIYGDGYLTIEI
                     RTAEKYVEIFK"
     gene            913..1989
                     /gene="prfA"
                     /locus_tag="VFX34_07275"
     CDS             913..1989
                     /gene="prfA"
                     /locus_tag="VFX34_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5564746.1"
                     /translation="MFERLQAVEDRYERLNELLSDPDIVSDMTKLRTYSKEQSDLQDT
                     VDAYREYKSATTQYDNAKEMLEEPLDDEMKELVKMEIAELDDQIESLEQRLKLLLVPK
                     DPNDDKNVIMEIRGAAGGDEAALFAGNLYRMYSRFAETNHWKVEVMDSSPTELGGFKE
                     IIFMINGNGAYSKLKYENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEK
                     DIRTDTFASSGPGGQSVNTTMSAVRLTHLPTGITVSCQDEKSQIKNKEKAMKVLRARI
                     YDKFMQEAQAEYDEKRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQIIEGK
                     LDEVIDALIVEDQARRLESLDQHD"
     gene            1982..2830
                     /gene="prmC"
                     /locus_tag="VFX34_07280"
     CDS             1982..2830
                     /gene="prmC"
                     /locus_tag="VFX34_07280"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5564747.1"
                     /translation="MTEKIFEALNKAKSLLTSKNLDENAAQLLMEHVTQKSSAALLAD
                     LREPLTPEQHTAFWDKTEELLTGRPVQHIIGTEQFYGLSFEVNEHVLIPRPETEELVW
                     GALERSRNFLKECRVADIGTGSGAIAIAFKKERPDALVSATDISEAALKVAKRNAERN
                     EAEIDFRLGNLTEPINGEKWDIVLSNPPYIAMEESEKMSPTVLDFEPHNALFADDDGL
                     FFYKQLAKKLPKLMDKPSLIGVEIGYLQGPAVSELFQKAFPHARVSVVKDVNGKDRMI
                     FCEIDE"
     gene            2874..3452
                     /locus_tag="VFX34_07285"
     CDS             2874..3452
                     /locus_tag="VFX34_07285"
                     /inference="COORDINATES: protein motif:HMM:NF021093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HEX5564748.1"
                     /translation="MLQDYEITNLTNLKPNKRMDRLMAVVGFILVLFLIQSALLLFTG
                     QAGQEEEIRFRILSHSNAPGDQKLKEEIRLAVEPMIQQAISQAESKSEIVDNLKAIES
                     AVLQTAKSMADGQDVSFERTAALFPPKRSGLVITPQAPYDAYILMIGSGRGDNWWCSL
                     FPRVCFPDKDVDKKEDEKVTFFIWEWIKSLFD"
     gene            3513..>4485
                     /locus_tag="VFX34_07290"
     CDS             3513..>4485
                     /locus_tag="VFX34_07290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5564749.1"
                     /translation="METEIVSVDNDMDNLLSYQRAVDILKAGGVVAFPTETVYGLGAL
                     ATDAEAVQRIFEAKGRPSDNPLIVHIGYAEDVHNYAIDVPESAEDLIDAFWPGPLTLV
                     FHKRPGVIAENVSPGFNTVGLRMPEHPVALQLLRTLNGPLAAPSANRSGKPSPTEAGH
                     VLTDLDGRIPLIVDGGQTGVGVESTVIDMTANPPEILRPGGVTREMIEEVIGPVRAES
                     KSPSASDEEAPRAPGMKYMHYAPESPLFVIQPDEAVIREAVRQLHSEGKRVAVIGPDD
                     FEIAEADWYFPVGPLHDNEAMAGNLYGAIRKCDLTDADLILAAATNHD"
BASE COUNT         1208 a         1099 c         1232 g          946 t
ORIGIN      
        1 agtattcgaa agcggctaca atcctgaaga tgcccctcat ctgggagaag ttacaatcgc
       61 tacgtatacg acttacttca agccatcaga cacgaaccgg aataaaaaca tcgaactttc
      121 cgcgaaatcg atcaacaata ctatcgtcgg cagcggagac caattctcct tcaacaccgt
      181 cgtcggaccg cgcgacgaag cgagcggcta ccagccggca ccggaaatca tcaacaaaaa
      241 acttgtcatg ggaataggcg gaggagtttg ccaaacatcc tccactgtat tcaacgctgt
      301 cgatcagctt caagtgaaat acatcgaatg gcatcatcat tcaatcgaca tcggctacgt
      361 cccgaaaggc agagatgcaa ccgtctccta cggcggtctc gacttcagat tccagaacac
      421 cgcggaagcc cctttcttga tcaagaccat ctatggggat ggctatctga cgattgaaat
      481 caggacggcg gagaaatatg tggagatttt caagtgaatc agcatgccgg ttcacttgac
      541 cattcacaat cgaagttttg ctcgagttcg atggagttat gctcgttttg tagccaatta
      601 tgctcgagtt cgggggtgtt atgctcgagt ttgacctagt tttgctcgtc ttgggactta
      661 agactcttcc gatatctcac aaaccttagt ttgctaaagg catttggcgc gaaaaagtgt
      721 gtaaacagat atgaaaatcc ttccgcgtat aatgatcgcg gctaaacact aagaaccgcc
      781 acttatcagc tcgcgccaaa tgcacttatc catccaccca gccctccatc caaagaaatc
      841 actgtatttc atcgccttat tcccgtacaa taaacataca aaacagactg aggacgaaga
      901 ggtgcaatga ctatgtttga aaggcttcaa gccgttgagg atcggtatga gcgattgaat
      961 gaattgctga gcgatccgga tattgtaagt gatatgacca agctgaggac gtattcgaag
     1021 gagcaatcgg acctacagga tacggtcgat gcgtaccgtg aatataagag cgcgacgacg
     1081 cagtacgaca atgcgaagga aatgctggag gagccgctcg acgatgaaat gaaggaactc
     1141 gtcaaaatgg aaatcgcgga gctcgatgat cagatcgaat cgcttgaaca gaggctgaaa
     1201 ctattgctcg tcccgaaaga tccgaacgat gacaagaacg tcatcatgga aatccgcgga
     1261 gcggcgggcg gtgacgaggc ggcgctgttc gcgggcaatt tgtacaggat gtacagccgg
     1321 ttcgcggaga cgaaccattg gaaagtcgaa gtgatggatt cctcgccgac cgagctcggc
     1381 ggcttcaagg aaatcatttt catgatcaac gggaatggcg cgtattccaa gctgaaatat
     1441 gaaaacggcg cgcatcgcgt acagcgtgtg ccggaaacgg aatcgggcgg ccggatccat
     1501 acgtcgaccg ccactgtcgc cgttttgccg gaagcggagg aggtcgaaat cgacatccac
     1561 gaaaaagaca tccgcactga cacatttgct tcgtccggac cgggcgggca atccgtcaac
     1621 acgacgatgt ccgctgtccg cctgacgcat ttgccgacgg gcatcaccgt ctcgtgccag
     1681 gatgaaaaat cacagatcaa aaacaaggag aaggcgatga aagtcctccg tgcgcgcatc
     1741 tacgacaagt tcatgcagga agcacaagcc gaatacgacg aaaagcggaa gtccgccgtc
     1801 gggacgggcg accgctccga acggatccgt acgtacaatt tcccgcagaa ccgggtgacg
     1861 gaccaccgga tcggactgac aatccaaaaa ctcgaccaga ttatcgaagg gaagctcgac
     1921 gaagtgatcg acgcccttat cgtagaagat caggcgagac ggttggaaag cttggatcag
     1981 catgactgaa aaaatctttg aagcgctcaa taaagcgaaa tcccttctga catcgaaaaa
     2041 cttggacgaa aacgcagccc agctgctcat ggaacacgtc acacaaaaat cgagcgccgc
     2101 tttgcttgct gatttaaggg aaccgctcac acctgaacag cacaccgctt tctgggataa
     2161 aaccgaagag ctgcttacag gcaggcccgt ccagcatatt atcggcacgg aacagtttta
     2221 cggactgtca ttcgaagtga atgaacatgt gctcatccct cggcccgaaa cggaggagct
     2281 cgtatggggc gccctggaac gaagccgcaa tttcctcaaa gaatgccgcg tggcggatat
     2341 cggtacaggg agcggcgcaa tcgcgattgc cttcaaaaaa gaaagacctg acgcactcgt
     2401 ctcggcgacg gatatcagtg aggcggcact caaggttgcg aagcgcaatg ccgagcgcaa
     2461 tgaagccgaa attgatttca ggctcggcaa tctgacggag ccgattaatg gcgagaaatg
     2521 ggatatcgta ctctcgaacc cgccgtatat cgcgatggaa gaatcggaaa agatgtcccc
     2581 gacagtgctc gatttcgaac cgcataatgc gcttttcgcg gatgatgacg ggcttttctt
     2641 ctataaacag cttgcgaaga aattgccgaa gctcatggac aaaccgtcgc tcatcggcgt
     2701 cgaaatcggc tacttgcaag gaccggcagt gagcgaactg ttccaaaagg cgttcccgca
     2761 tgcacgggtt tccgtcgtga aagatgtgaa cggaaaagat cggatgatat tttgcgagat
     2821 tgatgaataa aaccccctga tcctggtgac aatgtggtca ggaggggatc gatatgttgc
     2881 aagactatga aattacgaat ctaacaaacc ttaaaccaaa taagcggatg gatcgtttga
     2941 tggcggtagt cgggttcatt ctcgtgctgt tcctcatcca gtccgcattg ctgttattta
     3001 caggacaggc aggccaggaa gaggagatca gattccggat cctgtcccac tcgaatgccc
     3061 caggtgacca gaagttgaag gaagaaatcc ggcttgccgt cgagccgatg atccaacaag
     3121 cgatttctca agcggagtcg aaatccgaga ttgtggataa tttgaaggcg attgaatcgg
     3181 ccgttttaca gaccgcgaaa tcgatggcgg acgggcagga cgtaagcttc gaacggacgg
     3241 cggctttgtt cccgccgaaa cgttccggct tggttatcac gccgcaagcg ccttatgacg
     3301 cgtatatttt gatgatcggc agcgggcggg gcgacaattg gtggtgctct ttattcccga
     3361 gagtctgttt tccggacaaa gatgtggata aaaaagaaga tgaaaaggtc actttcttca
     3421 tttgggagtg gataaagtcg ttattcgatt gaagttatcc acttgaattg tggataagtg
     3481 gatgaaaatg tgtataaatg ggggtttcaa ccgtggaaac tgaaatcgtt tcagtggata
     3541 acgatatgga taacttgcta agttatcaac gggctgtgga tatattgaaa gcgggaggcg
     3601 ttgtcgcgtt tccgaccgaa acggtgtacg gcctcggagc gcttgcgaca gatgctgaag
     3661 ctgtgcagcg gatctttgaa gcgaaggggc gcccgtccga caacccgctc atcgtacata
     3721 ttggatatgc agaagatgtg cataactatg caattgatgt gccagaaagt gcagaagatc
     3781 ttatagatgc attttggcct ggcccactga cgctcgtctt ccataagaga cccggtgtca
     3841 ttgcggaaaa tgtctcgccg ggcttcaaca cggtcggact gcggatgccc gagcatccgg
     3901 tcgcgctgca acttctacgt acgcttaatg gaccgcttgc cgctccgagc gcgaatcgca
     3961 gcggaaagcc gagcccgacg gaagccggac atgtgttgac cgaccttgat ggcaggattc
     4021 cgctcatcgt cgacggcggt cagacgggtg tcggtgtcga atcgaccgta attgacatga
     4081 cggcgaatcc gccggaaatc ctccgtccag gcggcgtgac acgcgagatg atcgaggaag
     4141 tgatcggacc tgttcgcgca gaaagcaagt cgccaagtgc ctccgatgaa gaggcgccgc
     4201 gtgcacccgg catgaaatac atgcattacg cgccggaatc gccgcttttc gtcattcagc
     4261 cggacgaagc ggtaatccgg gaagcggtca ggcaacttca ttccgaaggg aagcgggtcg
     4321 ccgtcatcgg tcctgacgac tttgagatag cagaggcgga ctggtatttt ccggtcggtc
     4381 cgctccatga caatgaggca atggcgggga atttatacgg ggcgatccgg aaatgcgatt
     4441 tgacggacgc tgatctcatc ctcgccgcag cgacgaatca cgatg
//
LOCUS       DASULI010000341         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1567075,
            whole genome shotgun sequence.
ACCESSION   DASULI010000341 DASULI010000000
VERSION     DASULI010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567075"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1812
                     /locus_tag="VFX34_07295"
     CDS             163..1812
                     /locus_tag="VFX34_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5564750.1"
                     /translation="MKSANQVDRTELPLVMDSLIEFLMVTRTDGEGLITYTNHNFLAS
                     SNWTPKRILGKSFWQMFSEDEHSQAQAHAIWNRVMNGKTWFGKVEKLTRHGDPYYVQM
                     MAIPVLDTEGNLASCTFFELDITEEVELKEKLQQIAFIDFETGLMSRHKLETLVNEYI
                     QEGRHFSFVYIAIDHYYTLKDLQSYDSESVLIQEFTNRMKRYFQGDPIARIGVSQFVV
                     LTSFGDWFVQGFLAFLKEQPIYVKNHSLPLSVSGGIVRFPEDQLSYSHLYKAALAATK
                     TITEQGGGRIASLSAESHKVLNRRSLIDKKMLTALDHNNLQVVYQPQLDIATGKVLVY
                     EALVRWEDEEIGHIQPDELIPIAEENGLIHAIGAYVLEESARLAVKLMADKPDIRISV
                     NSSVREFGDSSQMKEKLSGILEKTKCPADKLELEITEKFAFQAEQEHAIIQQMKAFEE
                     TGIRFILDDFGTGYASFRYMQQLPISKVKIDKVFINSLLTMPKTRQLVEGMILFGKSM
                     GLYVIAEGVETEEQFDALKEMGIDAVQGYYIGGPVKGNEIS"
     gene            complement(1988..>2474)
                     /locus_tag="VFX34_07300"
     CDS             complement(1988..>2474)
                     /locus_tag="VFX34_07300"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX5564751.1"
                     /translation="FGYETVAQKNPSGGEPLILTKGMKISLFDVTDFHNPKEKAIEVI
                     GGRGTYSALQYDHKALFQHKKRNLFGFPVSIYEEGAEEFEVDYKGSGALVYEITPDRG
                     IVLKGDLVKQKSSAEQYEDWEKQIQRLVYSGDSVFTLSMNEIKSYKLDSFNEISTLKM
                     K"
BASE COUNT          661 a          573 c          585 g          655 t
ORIGIN      
        1 atgatcatga aaatcccttt tgctctttcc atatcactcc accacctttt ctagttgtct
       61 agtaactctt gatgtgctca ttccgccata aaagatgtac tgtaaattta ctgaacttcc
      121 tatataataa aatacatatg acatccgaaa ggggacaaaa agatgaaatc ggcaaaccaa
      181 gtagatagaa ccgagctgcc gcttgtaatg gacagtctaa ttgagttctt aatggtgacc
      241 cgtacggatg gtgaaggctt gatcacatat acgaatcata acttcttagc ttcaagcaat
      301 tggacaccaa aacgtattct cggaaaatcc ttttggcaaa tgttttccga ggatgaacat
      361 agccaagcgc aggcacatgc gatatggaac cgtgtaatga acggtaaaac ctggttcggc
      421 aaagtcgaaa agctgacccg gcatggcgat ccctattacg tccagatgat ggcaatcccc
      481 gtacttgata cggagggtaa cctcgcgtcc tgcacgtttt ttgagcttga tattacagaa
      541 gaagttgagc tgaaggagaa actccagcaa atcgccttca tcgattttga gaccgggctc
      601 atgagccggc acaaattgga gacgttggtc aatgaataca ttcaggaggg ccggcacttt
      661 tcgttcgtct acatagcaat cgatcactat tatacactga aagatctaca gtcgtatgat
      721 tcggaatcgg ttttgatcca agaatttacg aaccggatga aacgatattt ccaaggggat
      781 ccgattgcaa ggatcggcgt cagtcaattt gtcgtattga cgtctttcgg agattggttc
      841 gtccaaggat tccttgcgtt tttgaaagag cagccgatct atgttaaaaa ccactctttg
      901 ccgttatcgg tgagcggcgg aatcgtccgc ttcccggagg accagctctc ctattcacat
      961 ctgtacaagg ctgcattggc agcgacgaag acgatcactg agcaaggcgg cggacgtatc
     1021 gcctcattat ccgcggagtc ccataaagtg ttgaaccgcc gttcgctcat cgacaaaaag
     1081 atgctgacgg cgcttgacca taacaacttg caagtcgtct atcagccgca attggatatc
     1141 gcgacgggca aggttcttgt gtacgaagcg cttgtccgtt gggaagacga ggaaatcggg
     1201 catatccagc cggatgaact gattccaatt gcagaggaaa acggtttgat tcacgcaatt
     1261 ggggcctacg tgcttgagga gtcggcaaga cttgcggtga aactgatggc cgataagccg
     1321 gatatccgca tttccgtcaa ttcatccgtg cgcgagttcg gcgactcttc acaaatgaag
     1381 gaaaagctgt cgggcattct tgagaagacg aagtgccccg cggataagct cgagttggaa
     1441 atcacggaga aattcgcatt ccaagccgag caggaacatg caatcattca acaaatgaaa
     1501 gcgtttgaag agacaggcat ccgtttcatc ttggatgatt tcggaacggg ctatgcctct
     1561 ttccgttaca tgcagcaatt gcccatatcc aaagtgaaaa tcgataaagt gttcatcaat
     1621 tctctgctga cgatgcctaa aacacggcag ctcgtcgaag ggatgatcct tttcggtaaa
     1681 tcgatgggcc tttatgtcat tgcagaaggc gtcgaaaccg aggaacagtt cgatgcattg
     1741 aaggaaatgg gcattgatgc ggttcaaggc tattatatcg gaggccctgt aaaagggaat
     1801 gagatttcat aagaaaagga gccgctcggg agcggatcct ttttttgttg tctgcgtgat
     1861 taattgtgct cctgatgaaa taattgaacc cttgcgcgat taattgagcc tttgttgaaa
     1921 tatttgagcc gttgcaggtt ttcgacataa aacagaggga gcgcaagcgc tccctccacc
     1981 ctcttcatca tttcatcttc aacgtgctga tttcattaaa ggagtcaagc ttgtaacttt
     2041 tgatttcatt catcgataaa gtgaatacgc tgtccccgct gtacacgaga cgctggatct
     2101 gcttctccca atcctcgtat tgttcggctg acgatttctg tttcaccaaa tcgcctttca
     2161 gcacgatccc tcgatcaggt gtaatttcat agacgagtgc tccggaaccc ttatagtcca
     2221 cctcaaattc ctcggcacct tcttcataaa tactgaccgg gaagccgaag aggtttctct
     2281 ttttatgctg gaacagcgct ttgtggtcgt attggagtgc cgaatacgta ccccgtcccc
     2341 cgatgacctc tatggccttc tctttcggat tatgaaaatc ggtgacatcg aacagtgaga
     2401 ttttcatccc tttcgtcaaa atcaatggct cgccaccgga cggatttttc tgcgcgaccg
     2461 tttcatatcc gaag
//
LOCUS       DASULI010000342         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1625123,
            whole genome shotgun sequence.
ACCESSION   DASULI010000342 DASULI010000000
VERSION     DASULI010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625123"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1539
                     /locus_tag="VFX34_07305"
     CDS             199..1539
                     /locus_tag="VFX34_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX5564752.1"
                     /translation="MYRYKNVMATIGITSIVILILTSSAAVASEFGFDKKFADHTYIG
                     PFNISNNKTKQAKSKLGSDLSDLHTKLEVNIVYQDIQFSLPAETITFDVDMTLVNANS
                     GEDNPIIASVSREGLKIVLRQELPGIHITDNAIDSIATGIERDLQTGITPINVHISDY
                     IGRNEMPDEEVASSEYRIDGISPAFSKVIQALDKSIIRPFGSFSSMELVTSSGVGSIS
                     DEEMTLLSSLLYSAVLQTNFQIDERNRSTVLSPDIQPGFEAAINQSLGLDFQFTNPNK
                     TEFTIRAAWSAGAIRLTIEGKPFYYTYEPYVVNIETYKPRTIRQYSASVNDGQVDISE
                     QGKEGVEAVVKRTISIDGNVVETEVISEDFYAPIQRIEIHPLSKSASVSDTANSEESD
                     PSQNGGSSSEIEIDASELEEVESNNFDTDNATGSKELTEEEVVYDKSGLPLSGK"
     gene            1570..3228
                     /locus_tag="VFX34_07310"
     CDS             1570..3228
                     /locus_tag="VFX34_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003347625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX5564753.1"
                     /translation="MAITRKRLGDLLVESGLITDKQLQTALEEKPQDQKLGDALLQRG
                     YITEQQLIEVLEFQLGIPHVNLFRYPFDPKLFNVVPKEFAKRNLLVPLRADGDRLFVA
                     MSDPMDYLAVDDLRLSTGFHIETAIASKDDILRTISKYYEDDLLEDFFVEQAQETSDQ
                     QDELTDLDSPIVRLVNQLLSSAITQKASDIHIDPQEQQVVIRFRVDGMLKTERYLPKH
                     MQAMLTARIKIMSSLDITEQRVPQDGRIKATVDFRPIDLRVSTLPTVFGEKVVMRILD
                     LSSSLNDLKKLGFSPLNLERFMKEIERPNGIVLISGPTGSGKSSTLYAALNKLNKEEV
                     NIITVEDPVEYQLEGINQIQVNPAVGLTFANGLRSILRQDPDIVMVGEIRDRETVEIA
                     IRASLTGHLVLSTIHTNDSIASITRLIDMGVEPFLVTASVNAIIAQRLIRRVCRDCGK
                     EMPASNREKEIFAKRGKKIESIVRGPGCAACNMTGYRGRVAIHEVLMINEDMREVINN
                     HGTPAMLRDIAVRNDTVFLIDDGLEKVKQGITTTEEVLRVSLIE"
BASE COUNT          987 a          669 c          802 g          818 t
ORIGIN      
        1 catggttgca aatggaggta aacatgtaac aggtgagtta attcaagtat ctactgaaag
       61 tgaattgtgg gagaaatgta aattaggaaa tagtgatcca cttgtttgga caaagcctgt
      121 cctcgaagat gtgacatatg aatagtttcg cactattaaa gagaacagat ggacaggctg
      181 gatgggaggt ggagaaacgt gtacagatat aaaaacgtga tggcgacaat tggaattacc
      241 tctattgtta ttttgatatt aacgtcgagt gccgctgtag cgtcagaatt tggattcgat
      301 aaaaaattcg cggatcatac atacattggc cctttcaaca tatccaacaa taaaaccaaa
      361 caagctaagt caaaactagg ttcagattta tccgatctcc ataccaaact ggaagtgaac
      421 atagtctacc aagacattca attttcactt cctgctgaaa caatcacatt cgatgttgac
      481 atgactttag ttaatgcaaa ttccggggaa gacaacccaa tcattgcatc agtctcaaga
      541 gaaggcttga agattgttct cagacaggaa ttgcctggca ttcacattac agacaatgca
      601 attgattcaa ttgcaaccgg cattgaaaga gatttgcaaa caggtatcac gcctataaat
      661 gtccatattt cagattatat tgggcggaat gagatgcctg atgaggaagt agcgtcatct
      721 gaatatagaa ttgacggaat ttctccggct ttttcaaagg tgatccaagc attggataag
      781 tcgatcatcc gaccattcgg atcgttttcc tcgatggaat tggtgacaag ttccggagtg
      841 ggatcgatta gcgacgaaga aatgactttg ctttcttcac tattatacag cgccgttctt
      901 caaacgaatt tccagattga tgagcggaat aggagtactg tcctatcacc cgatatccaa
      961 ccaggcttcg aagcggcaat aaatcagtcg ttaggtctag acttccagtt cacgaacccg
     1021 aataagacgg aattcaccat ccgggccgct tggtcggcag gggcaatccg tctaacaatt
     1081 gaaggcaaac ctttctatta cacatatgaa ccttacgttg taaatattga aacgtataaa
     1141 ccgagaacca tccgacaata tagcgcatcc gtaaatgacg gacaagttga catttcggag
     1201 caaggcaaag aaggcgtgga agcggttgtg aaacggacaa tttccatcga tggaaatgtc
     1261 gtggaaacag aagtgatttc cgaagacttc tatgcaccaa ttcagagaat tgaaatccat
     1321 cctttatcca aaagtgcatc tgtgtcggat actgccaata gtgaagaaag cgacccttca
     1381 caaaacggcg gttcttcttc cgaaattgaa atagacgcta gcgagttgga agaagtggaa
     1441 tccaacaact tcgatactga taatgcgacc ggtagtaaag aattgacgga agaggaagtc
     1501 gtctatgata aaagcggcct tccgctatct ggaaaatgat agaagtcaag tagtgaaagg
     1561 gggatgtaaa tggcgataac acggaaaaga ttgggcgatc tgctcgttga atcaggtctc
     1621 attacagaca agcagcttca aaccgcattg gaagaaaagc cgcaggacca aaaacttggt
     1681 gatgcgcttc ttcagagagg ttatattaca gagcagcaat tgattgaggt gctggaattc
     1741 cagcttggaa ttccccacgt caatctattc aggtatccgt ttgatccgaa gctgttcaac
     1801 gtcgtgccaa aagaatttgc aaagcggaat ttgctcgttc cattaagagc tgatggagac
     1861 aggctgtttg ttgcgatgtc cgacccgatg gattatttgg ctgtcgatga cttgcgtttg
     1921 tcgacgggtt tccatattga gacggcgatt gcgtccaagg atgatatatt gcggacaatc
     1981 tcgaagtatt atgaagatga tctcctcgaa gactttttcg tcgaacaggc tcaggaaaca
     2041 agtgaccagc aagatgaact gactgatctt gattccccga tcgtccgtct cgtcaatcag
     2101 ttactgtcgt ccgcgatcac ccaaaaggcg agtgatatcc acatcgatcc ccaggagcaa
     2161 caagtcgtca tccggttccg ggtcgacggg atgttgaaga cggagaggta tttgccgaag
     2221 catatgcaag cgatgctgac cgcacggatt aaaatcatgt caagccttga tatcaccgaa
     2281 caacgcgtac cgcaagatgg aaggattaaa gcgacggtcg atttccggcc gatcgatctg
     2341 cgggtatcga cgttgccgac ggtatttggc gaaaaagttg tcatgcggat tttggatctg
     2401 agcagttctt tgaatgactt gaagaaactg gggttcagcc ccttgaattt ggaacggttc
     2461 atgaaagaga tagaacgtcc gaatggcatc gtcctcattt caggaccgac cggttctggg
     2521 aaatcgtcga cgttatacgc agctttgaat aagttgaaca aagaagaagt gaacattatc
     2581 accgttgaag atccggtgga atatcaactc gagggcatca atcagattca agtgaatcct
     2641 gcagtcggcc tcacgttcgc aaatggctta cggtcaatcc tgagacagga tcccgacatt
     2701 gtcatggtcg gtgaaatccg tgaccgtgaa accgtggaaa tcgccatccg tgcatcattg
     2761 acaggccatc tcgtcctgag cacgatccat acgaatgatt ctatcgcatc gattacaagg
     2821 ctgattgaca tgggtgttga accttttcta gtgacggcct ccgtgaacgc cattatcgca
     2881 caacggctga tcagacgcgt ttgtcgggat tgcgggaagg aaatgcccgc ctcgaaccgt
     2941 gaaaaagaga tttttgccaa acgcggcaaa aagatcgaat cgattgtccg aggacctggc
     3001 tgtgcggctt gcaatatgac cggttaccga gggagagttg caatccacga agtgcttatg
     3061 atcaatgagg atatgcgtga agtgatcaat aaccatggaa caccggcaat gttacgggat
     3121 atagcggtgc ggaatgacac tgtatttttg attgacgatg gattggagaa agtgaagcag
     3181 ggcattacaa cgactgaaga agtgcttcgc gtttcactta ttgaataggg gtatatagcc
     3241 atgaatgaaa caatagatca tattttaaca aaagca
//
LOCUS       DASULI010000343         2456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1606006,
            whole genome shotgun sequence.
ACCESSION   DASULI010000343 DASULI010000000
VERSION     DASULI010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606006"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..996
                     /locus_tag="VFX34_07315"
     CDS             151..996
                     /locus_tag="VFX34_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014458599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5564754.1"
                     /translation="MKFHQPPHIYTGEVVLKVTDLDQLTSFYTEIIGFRVLKKEDNQV
                     VLTADGQRPLLVLEQPKKILPKEPRRTGLYHFALLLPTRADLGKAIKHFSKNEVRLGA
                     SDHLVSEALYLSDPDGNGIEIYRDRDPESWKWQNDFVAMSTDPLNAQAIVEASGDEEW
                     NGLPEGTLMGHVHLHVASLPETQAFYEALGFRVVTHYPQALFMSTGNYHHHLGLNTWN
                     GAGAPRPSQDSAGLKAYTLVYPDEATLKATIGKVEALGTTVESKEGLFVTEDPAGNGI
                     ILRIH"
     gene            1111..2190
                     /locus_tag="VFX34_07320"
     CDS             1111..2190
                     /locus_tag="VFX34_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004231912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5564755.1"
                     /translation="MTKKLWFQVGVGILLAILIIKYFMEISFIFAPVIIIIKAIILPL
                     LLGGVLYYMTEPIQRFLENRKVPRWGSILIILAGLVVVLGVFISIIGPQVTKQVNNLV
                     ENAPTLAKDFEVIKEKLLRQKEDLPESLQKSIDSAANSLQTIAVNFSKWVVQFLQSFF
                     QAIFLLVLVPFFFIFMLKDHEKFAPLIYNFFSGERREWVKKTLSDIDNVLRSYIQGQF
                     LISAILAAIIFVGYLFLGLQYALLLAIFALFMNLVPFIGPWIAVAPALIIAYMDSPKL
                     VIGVAIVTLVAQQIDSNLITPNVMGKSLDIHPLTVITIILAAGNIAGFVGIIVGVPVY
                     AVGKVIVRNIYDQRRKIKHAATKNV"
BASE COUNT          695 a          525 c          563 g          673 t
ORIGIN      
        1 ggaaaccgcc ttttcaaagc gtggacaaag ctcgaattca ttcatatgcc aagttccttt
       61 caaagtgaaa atcgtttgtg attgacattt catattatag catataaagt tagttacata
      121 aagtaagtaa cttaccagga ggaataaaac atgaaatttc atcaaccacc acatatatat
      181 acgggggaag ttgttttaaa agtgactgac ctggaccaat tgacaagctt ctatacggaa
      241 attatcggat tccgcgtgct gaaaaaagag gataaccaag ttgtattgac tgcagacggt
      301 caacgtccat tactagtcct agaacaaccg aagaagatcc ttccgaaaga accgagacgc
      361 acaggcctat atcatttcgc gcttttactg ccgacacgtg ctgatcttgg gaaagcgatc
      421 aaacactttt caaagaacga agtacgtctt ggggcgtctg atcacctcgt aagtgaagcg
      481 ctgtacttat ccgatcctga cgggaacggc atcgaaatct atcgcgatcg agatcctgaa
      541 tcatggaaat ggcaaaatga tttcgtcgcg atgagcaccg atccgcttaa cgcacaagcg
      601 attgttgaag caagcggaga tgaggaatgg aacggcctgc ccgaaggtac gctcatggga
      661 cacgtccatc tgcatgttgc gagtctccca gagacacaag ctttctacga agcgttgggg
      721 ttcagagtcg tcactcatta cccacaggca ctattcatgt cgaccggaaa ttatcaccat
      781 caccttggct tgaacacatg gaatggagca ggcgcgccac gtccatcgca agatagcgct
      841 ggattgaaag cttacacatt agtgtatcct gacgaagcga cactgaaggc taccattggt
      901 aaggttgaag ccctaggtac aactgtggaa tcaaaagagg gactctttgt gacggaagat
      961 ccggcgggga atggaatcat cctccgtatt cattaagaaa aataataaaa actaagtaca
     1021 gccaataatt ttgaaacttc tttcgacagg aaacgtaata aaccgtatac ttaattcatc
     1081 actatcgtag acggtaaagg acgtggaaaa ttgacgaaga agctttggtt ccaagttggc
     1141 gtcggcattt tattggccat cttaatcatt aaatatttca tggagatcag tttcatattt
     1201 gcgccggtca tcattattat aaaagcaatc atcttaccgc ttctgcttgg cggcgtgctt
     1261 tattacatga ccgaaccgat ccagcgtttt cttgaaaacc ggaaagtccc acgttgggga
     1321 agcattttga tcattttggc gggtcttgtc gttgtccttg gggttttcat ctccatcatc
     1381 gggccgcaag tgacgaaaca ggtgaataat cttgttgaga atgcgccgac attggcgaag
     1441 gactttgaag tcataaagga aaagttgttg cgacagaagg aagacttgcc ggaaagtctg
     1501 cagaaatcga ttgacagtgc tgcgaattcg ctgcagacga ttgccgtgaa tttcagcaaa
     1561 tgggtcgtcc aatttttgca atcctttttc caagcgatat tcctgctcgt attggtgcca
     1621 ttcttcttca tcttcatgtt gaaggatcat gaaaaattcg cacccctcat ttataacttc
     1681 ttcagcggcg aacgtcgcga atgggtgaag aagacattga gtgacattga caatgtgttg
     1741 cgctcttata tccagggcca atttctaatc agtgcaatat tggcagctat catttttgtc
     1801 gggtatttgt tccttggtct gcaatacgca ttgttgctag cgattttcgc gcttttcatg
     1861 aacttggtgc cattcatcgg gccatggatt gctgtcgcac cggcactcat cattgcgtat
     1921 atggacagcc ctaaacttgt cattggtgtc gcgatcgtca cgcttgtcgc ccagcagatc
     1981 gatagcaacc taatcacgcc gaacgtcatg ggcaagtcgc tcgacatcca cccgctcact
     2041 gtcatcacaa tcatccttgc ggcaggtaac attgccggtt ttgtcggcat catcgtcggg
     2101 gtgccagtat atgcggtcgg gaaagtgatt gtacggaata tttatgatca gcgaagaaaa
     2161 ataaaacatg ctgctacgaa gaatgtgtag aggtgcatgg acagtattca taaagcaatg
     2221 ctactaaccg gtagagaata ggaaggcaac catatatgct atggttgcct tttttctatt
     2281 gccaatcgat ttggccggtt cgtttacttc tcatacgtca atggaagcat gcgctatcat
     2341 acaacatcca tatttgtatt cttaagactg attttaacta atcgattgac aattcaatta
     2401 tttatgtgta atgtattatg tacagtgtat acattgcaca gaggagagat tggatg
//
LOCUS       DASULI010000344         6454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_40978, whole
            genome shotgun sequence.
ACCESSION   DASULI010000344 DASULI010000000
VERSION     DASULI010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40978"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFX34_07325"
     CDS             complement(<1..594)
                     /locus_tag="VFX34_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX5564756.1"
                     /translation="MAESTPRVPQATSKRLPLYYRFLQNFANAGKRRVSSSELSEAMK
                     IDAATIRRDFSHFGALGRKGYGYDVNDLLLFFRRALDQDEETDVALIGVGSLGSAFLK
                     YNFQKNHNTRIVVAFDPKAPREGKTISNIPVFHPDMMEEKIKELGINLVILTVPTRAA
                     QEIADRMVDIGVKGILNFTPVRLTAPDWMRIHTIDLSV"
     gene            721..2658
                     /locus_tag="VFX34_07330"
     CDS             721..2658
                     /locus_tag="VFX34_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX5564757.1"
                     /translation="MIVLQVNGITKSFSGTDILKNVRLEVQHRDRVALVGRNGAGKST
                     LLKIIAGEMTADSGDLIMPKDVQVGYLEQHAGLDSPLTVWEEMLTVFGPLLEMEKRLR
                     GLEAQMADPSVYENPQEYERVMKDYDALQIEFKDSGGYQYESDMRSVLHGMRFYPKDY
                     GKQVNLLSGGQKTRLALAKMLLSKPDLLILDEPTNHLDIETLGWLEKYLVSYEGAILI
                     VSHDRYFLDQIVTITYEVSRMKVTKYVGNYSAYLVEKAKNYERDKKLYERETSERAKL
                     EDFIQRNIARASTSKMAKSRRKLLERTDWMDAPDGDEKSASFSFAIERPSGNDVLKLE
                     NIAVGYDGKPVSEHINMHAYKGDRIAIIGPNGIGKSTLLKTIVKRQEPIEGTVRYGTN
                     VQFGYYDQNQATILGTGTVLHELWDEWPMMNEKDVRSVLGRFLFTGDDVEKPVASLSG
                     GEKARLSLAKLMLQKSNTLILDEPTNHLDLDSKEVLENALDDFPGTILFVSHDRYFIN
                     RIATKVIDLGPAGVTEYLGDYDYFIEKKNELEELNAEQIASQQPKQPRNGRNEEDKEF
                     KRQERRLTRAIAEVEESLGKLDAEIAVLQEELLNPVFADDHVKLMELQEQIDELQMQH
                     DNLSEEWLNLQEELENLNSQT"
     gene            complement(3107..4129)
                     /gene="tsaD"
                     /locus_tag="VFX34_07335"
     CDS             complement(3107..4129)
                     /gene="tsaD"
                     /locus_tag="VFX34_07335"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5564758.1"
                     /translation="MDKDMYILGIETSCDETAASVVKNGHKIVSNVVASQIQSQQRFG
                     GVVPEIASRHHVEQITLVIEEALAKAELSPSDLDAVAVTEGPGLVGALLIGINAAKAF
                     AFANGLPIIGVHHIAGHIYANQLMQPMEFPLLALIVSGGHTELVLMENHGSFKLIGET
                     RDDAAGEAYDKVARVLGLPYPGGPHIDKLALESDEAVDFPRAWLEPDSYDFSFSGLKS
                     AVINYKHNLEQKGGEVNPSHVAAGFQQSVVEVLTEKTLRAAKEYGVKQVIAAGGVAAN
                     KGLRSSLDAVFEKEGIPFYVPPIYLCTDNAAMIAAAGTSMFESGKQGTMEMNGRPGME
                     LASWNE"
     gene            complement(4129..4578)
                     /gene="rimI"
                     /locus_tag="VFX34_07340"
     CDS             complement(4129..4578)
                     /gene="rimI"
                     /locus_tag="VFX34_07340"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5564759.1"
                     /translation="MNKQIEYRKMTVEDIPAVVEIEQEAFATPWTQEIFEHEMAGNDY
                     AHYIVAVVDGEVIGHFGMWVVLDESHITNVAVRMHMRGHGIGEALMRKAMELCKANEV
                     RVMTLEVRVSNHTAQNLYRKLGFQDGGIRKNYYTDDHEDALVMWVEL"
     gene            complement(4571..5281)
                     /gene="tsaB"
                     /locus_tag="VFX34_07345"
     CDS             complement(4571..5281)
                     /gene="tsaB"
                     /locus_tag="VFX34_07345"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX5564760.1"
                     /translation="MIWLGIDTANTPLSVAIVKDGAILAEVNSSMAVNHSLRAMPVIE
                     ELFATVKLQPKDIDAIAVSEGPGSYTGVRIGVTIAKTLAWTLKKPLFGVSSLKVLAAN
                     AFYFDGLICPILDARRNNVYAGVYEFVDGGLTAIAEDGHFAIENLLEGLASKDRPVLF
                     VGKDVAMHEELIVERLKENAVIGPFPMHLPRASSLIFIAEKSGQEENTHAFTPEYRRI
                     AEAEANWLMQQRKEQGSE"
     gene            complement(5278..5730)
                     /gene="tsaE"
                     /locus_tag="VFX34_07350"
     CDS             complement(5278..5730)
                     /gene="tsaE"
                     /locus_tag="VFX34_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010291027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX5564761.1"
                     /translation="MTIQFNVHSLAETEQIADTLAGLLSPPDVVTLEGDLGAGKTTFT
                     KALARSLGITRTVNSPTFTILKQYEGNVPFNHLDVYRLADSDEDLGWDELFYGDAVSV
                     VEWAHLIEDDLPEERLEIQIHHTGESSRKLVFIPKGERFVKICEAMTL"
     gene            complement(5978..6053)
                     /locus_tag="VFX34_07355"
     tRNA            complement(5978..6053)
                     /locus_tag="VFX34_07355"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6018..6020),aa:Asp,seq:gtc)
     gene            complement(6155..6270)
                     /gene="rrf"
                     /locus_tag="VFX34_07360"
     rRNA            complement(6155..6270)
                     /gene="rrf"
                     /locus_tag="VFX34_07360"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(6356..>6454)
                     /locus_tag="VFX34_07365"
     rRNA            complement(6356..>6454)
                     /locus_tag="VFX34_07365"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1659 a         1725 c         1485 g         1585 t
ORIGIN      
        1 caccgacaga tcaatcgtat ggatgcgcat ccagtcgggt gctgtcagcc ggacaggtgt
       61 gaaattgagg atccctttca cgccgatatc gaccatccga tccgcaatct cctgcgcagc
      121 ccttgtcgga actgtcaaga tgacgagatt aatgcccaac tccttgatct tctcctccat
      181 catatctgga tgaaaaaccg ggatattgct gatcgtcttc ccttctcttg gcgctttcgg
      241 atcgaacgcg acaacgattc gcgtattatg gttcttctgg aaattatatt tcaaaaatgc
      301 actccctaag cttccgacgc cgatcaatgc gacatccgtc tcttcgtcct ggtcgagcgc
      361 ccttcggaag aaaagtagga gatcattcac atcatagccg tagcctttgc gcccaagcgc
      421 tccgaaatgg gagaagtcgc gacgtatcgt agcggcatcg attttcatcg cctcgcttaa
      481 ctcactggaa gagacgcgtc tctttcccgc gttcgcgaag ttttgtagga atctataata
      541 taaaggaaga cgttttgacg tcgcctgtgg aactctcgga gtcgattcag ccatttctta
      601 cctcctatat acgggcattt agcctatact atcgtacagt agatgctagc tgatgtaaag
      661 ccgaccattg caccggcccg tccattcgat tacactaaaa gtggaataga ggtgtgctga
      721 atgatcgtgt tacaagtgaa tggaataacg aaatcctttt caggaactga catattgaaa
      781 aacgtccgcc tcgaagtgca gcaccgtgat cgggttgcgc tcgtaggacg aaatggcgcg
      841 ggcaaatcga cattgctcaa aatcattgca ggagagatga ctgcggattc cggcgatttg
      901 atcatgccga aagatgtaca agtcggctat ctcgaacagc atgcgggcct tgattccccg
      961 ctgactgtct gggaggagat gctgaccgtt ttcggaccgc tgcttgaaat ggaaaaaaga
     1021 ctccgcggcc tcgaagcaca aatggcggac ccttctgttt atgaaaatcc gcaagaatac
     1081 gagcgggtca tgaaggatta tgatgcgctc caaatcgaat tcaaggattc gggcgggtat
     1141 caatatgaat ccgacatgcg ctcggtcctc cacgggatgc gcttttatcc aaaggattac
     1201 gggaagcagg tcaatctgtt gtcgggcgga caaaaaacgc ggctggcgct cgcgaaaatg
     1261 ctgctcagca agccggatct cctcatcttg gacgagccga cgaaccattt ggatatcgag
     1321 acgctcggct ggctcgaaaa ataccttgtc tcctatgaag gggcaatcct tatcgtttcc
     1381 catgaccggt atttcctcga ccagatcgtg acgatcactt acgaagtctc aaggatgaaa
     1441 gtgacgaaat acgtcggcaa ctatagcgcc tatttggtcg agaaggcgaa aaactatgag
     1501 cgggacaaga agctgtatga gcgcgagacg agcgaaaggg caaagctaga ggatttcatc
     1561 caacggaata tcgcccgtgc ttcgacttcc aaaatggcga aaagccgtcg gaaactgctt
     1621 gaacgcacgg attggatgga cgcgccagat ggggatgaaa agtccgcaag cttctccttc
     1681 gccatcgaaa ggccaagcgg caatgatgtg ctcaaattgg aaaacatcgc tgtcggttat
     1741 gatggcaagc ctgtttccga gcatatcaac atgcatgcat acaaagggga ccggattgca
     1801 atcatcgggc cgaatgggat cggcaagtcg acgttgctga agacgatcgt gaaaagacag
     1861 gaaccgatcg agggaacggt ccggtacggg acgaatgtcc aattcggcta ttacgatcag
     1921 aaccaagcga cgatcctcgg tacggggacg gtcctgcatg agctttggga tgaatggcca
     1981 atgatgaatg aaaaggatgt ccgctcggtg ctcggacgtt tcctgttcac aggggatgac
     2041 gtcgagaagc ccgtcgcttc cttatccgga ggcgagaagg cgcgtctgtc attggcgaaa
     2101 ctcatgcttc aaaaatcgaa cacgctcata cttgacgagc cgacgaacca tctcgacctt
     2161 gacagcaagg aagtgctcga aaatgcgctg gacgactttc cgggcacaat ccttttcgtc
     2221 tcccatgacc ggtacttcat caaccggatc gccacaaaag tgatcgatct cggtcctgca
     2281 ggcgtcacgg agtatctcgg agattatgat tacttcatcg aaaagaaaaa tgagctggaa
     2341 gagctgaatg ccgagcagat tgcttcacaa cagccaaaac agcctagaaa tggacggaat
     2401 gaagaagata aggaattcaa acggcaggag cgccgattga cgagggcgat tgccgaagtt
     2461 gaagagtctc tcgggaaatt ggatgctgaa attgccgttc tgcaggaaga attgctgaat
     2521 cctgtctttg ccgacgacca tgtgaaacta atggagctgc aggaacaaat cgacgaactg
     2581 caaatgcagc acgacaacct gtcagaggaa tggttgaatt tgcaggaaga gctcgagaat
     2641 ttgaattcgc aaacctgata cagtgaaact tgaatcagtg ggatctttcc cccactgatt
     2701 gtaagttgaa ccaatcgggc ttttactggc tgttgattcc ctactgggaa tcttatagcc
     2761 agataaagcg ggataatcgc tgtcgaaggg cacagacgcc cattcgacag ctttttttag
     2821 ttttcgacaa acctgccatt tgatttccac aattttatca acagcatccg cgttgatata
     2881 atgggatatg aacatagtta tccacgttat ccacatttcc tgttgcgatt accaacatag
     2941 ttatccaaaa catgtaaggg tcattcacac aaatctttta actatccaca acttatcaac
     3001 aattgtgcat aagtacgaac gttccctctt gacaagtcgg attcgttgta ggacaaataa
     3061 aaacggaaat cgtcacaact cgacgatttc catcattcgt tctttctcat tcgttccatg
     3121 aggcaagctc catgcccggc ctgccgttca tttccatcgt tccctgtttt ccgctctcga
     3181 acatcgatgt acctgccgcc gcaatcatgg ccgcattatc cgtacataag tagatgggcg
     3241 gtacgtaaaa tgggatcccc tctttttcaa atacagcgtc caatgatgac cgcaagcctt
     3301 tattggctgc tacaccgccc gccgcgatga cttgctttac accgtattcc ttcgcggctc
     3361 ttaatgtctt ctccgtcaac acttcgacga cgctttgctg gaacccggcc gccacatggg
     3421 aaggattgac ttcgccgcct ttttgctcaa gattatgctt gtaattgata acggctgatt
     3481 tcaaaccgct gaagctgaaa tcataggaat ccggctcaag ccaagccctc gggaaatcga
     3541 ccgcttcatc gctctcaagc gcaagcttat cgatatgcgg acctcccgga tatggcagcc
     3601 caagaacacg tgcgactttg tcgtacgctt cccccgccgc atcatcccgc gtttcgccga
     3661 tcaatttgaa tgaaccgtga ttctccatga gaaccagctc cgtatgcccc ccggaaacaa
     3721 taagtgcaag cagcgggaac tccatcggct gcatcaattg attcgcgtaa atatgccccg
     3781 cgatatgatg cacgccgata atcggcagcc cgttggcaaa cgcgaacgcc ttcgccgcgt
     3841 tgatgccgat caataatgca ccgacaagtc ctggcccttc cgtcacagcg accgcgtcca
     3901 aatccgatgg cgacaattcc gccttcgcca acgcttcctc gattacgagc gtaatctgct
     3961 caacatggtg ccgtgacgcg atttcgggca cgacgccgcc gaagcgttgc tggctctgga
     4021 tttgcgaagc aacgacgttc gagacgatct tatgtccgtt cttcacgacc gaagccgccg
     4081 tctcatcaca actcgtttca attcctaata tatacatatc tttgtccatt acaattccac
     4141 ccacatgacg agcgcatctt catggtcgtc cgtataatag tttttccgta tgccgccatc
     4201 ttggaagccg agcttgcggt acaagttctg cgctgtatga ttgctcacgc ggacttcgag
     4261 cgtcatcaca cgcacctcat tcgccttgca cagctccatc gccttgcgca tgagcgcctc
     4321 gccgattcca tggcctctca tatgcatcct caccgccaca ttggtaatat ggctttcgtc
     4381 cagcacgacc cacataccga aatgcccgat cacttcgccg tccacgacgg cgactatata
     4441 atgggcataa tcattcccgg ccatctcatg ttcgaagatt tcctgtgtcc atggcgtcgc
     4501 gaatgcctcc tgctcgattt caacaactgc agggatatct tccactgtca tctttcgata
     4561 ttcgatttgt ttattcactg ccctgttcct tccgttgctg catcagccaa ttcgcctccg
     4621 cctcagcgat ccggcggtat tccggtgtaa atgcatgtgt attctcttcc tgaccactct
     4681 tctccgcgat gaaaatcaat gaagacgccc gcggaagatg catcgggaat ggtccaatga
     4741 cagcattctc tttcaatctc tcaacgatga gctcttcatg catcgcaacg tccttaccaa
     4801 caaaaagaac tggtcgatcc ttgcttgcta aaccctctag cagattttca atcgcaaaat
     4861 gcccgtcctc tgcgattgcg gtcaatccac catccacgaa ttcataaacc cctgcgtaca
     4921 cattattcct tcttgcgtcc agaatcgggc aaatcaaccc atcaaaataa aaagcattcg
     4981 cagcgagcac tttcaagctt gagacaccga acagaggctt cttcaacgtc caagccaacg
     5041 tcttcgcaat cgtcaccccg atgcggactc ccgtatagga gccagggcct tccgataccg
     5101 ctatggcatc gatatcttta ggctgcagct tcaccgttgc gaacagctcc tcaatgaccg
     5161 gcatcgcccg caatgaatga ttgacagcca tcgacgaatt cacttcggcc aagatagccc
     5221 cgtctttcac aattgcaaca gacaaaggcg tgttcgcagt atctattcct aaccatatca
     5281 taacgtcatc gcctcacaaa tctttacaaa acgttcccct tttggaatga acacaagctt
     5341 tctcgaagat tccccggtat ggtgaatctg gatttccaag cgttcttccg gtaagtcgtc
     5401 ctcaatcaga tgcgcccatt caacgacgga aaccgcatcc ccgtagaaca gctcgtccca
     5461 accaagatcc tcatcactgt ccgccaatcg atagacgtcc aaatggttga aaggcacatt
     5521 gccttcgtat tgcttcaaga ttgtaaatgt cgggctgttc accgttcgag taatgccaag
     5581 gctgcgtgca agcgccttcg tgaaggtcgt cttcccggca ccgagatcgc cttccaacgt
     5641 cacgacatcc ggaggggaaa gcaggcccgc caatgtatcc gcaatctgtt cagtttccgc
     5701 tagggaatga acattgaatt gtatagtcat cgtattttcc tttcatctcc actttgcttt
     5761 agtttagtgt accgaaaata atatgatttc gaaagccatg aggtttggtg gaggtatgta
     5821 attgatgttg aactgtttat tgcacggaat gccgctgcgc tgtgaaagaa tgttaacacg
     5881 cgctcaaaga acctgtgttt aataatagct tgcaaataaa acaacaaaaa accgatcctg
     5941 gaatgaatcc ataatcggtt gacagtttaa attaagatgg cggtcccgac cgggatcgaa
     6001 ccggcgatct cctgcgtgac aggcaggcat gttaaccgct acaccacggg accgtatgta
     6061 tacctttcga tagaatgcaa ggttctctaa ctgcaaattt attataattg atcaatttta
     6121 accaattgac attctgatcg gaaaggaatg ggttgcccgg cgacgtccta ctctcacagg
     6181 gggaagcccc ctactaccat cggcgctgaa gagcttaact tccgtgttcg gtatgggaac
     6241 gggtgtgacc tcttcgcctt cgtcaccaga catatatgaa cgacgcttgt tcgttcaaaa
     6301 ccggataaaa ctaacattgt cgctgaactc aagttcaacc gcacttcata acttcaaggt
     6361 taagtcctcg atcgattagt atccgtcagc tgcacgtgtc gccacgcttc caccccggac
     6421 ctatccacct catcttcttt gagggatctt actt
//
LOCUS       DASULI010000345         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1241693,
            whole genome shotgun sequence.
ACCESSION   DASULI010000345 DASULI010000000
VERSION     DASULI010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241693"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..88
                     /locus_tag="VFX34_07370"
     CDS             <1..88
                     /locus_tag="VFX34_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HEX5564762.1"
                     /translation="AMIHTPSIYVQGLLQAEQEKRIERLTTR"
     gene            98..742
                     /locus_tag="VFX34_07375"
     CDS             98..742
                     /locus_tag="VFX34_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX5564763.1"
                     /translation="MFTMNFRERIARRAEKEINDGDYVNLGIGMPTLVANYISDDKTV
                     VLQSENGLLGIGPYPTEDQVDPDLINAGKETVTTIPGSAFFNSAESFAMIRGGHVDVA
                     ILGGMEVSEKGDLANWMIPGKMIKGMGGAMDLVHGAKKIIVIMDHVAKDGSAKIKKEC
                     SLPLTGKGVVHKIITERALIDVTPDGLELKEVFEGFTVEDVINSTEAELKVNIG"
     misc_binding    1017..1234
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1306..>3715
                     /gene="leuS"
                     /locus_tag="VFX34_07380"
     CDS             1306..>3715
                     /gene="leuS"
                     /locus_tag="VFX34_07380"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5564764.1"
                     /translation="MSYKHVEIEKKWQQYWKDNKTYKMVNDDSKPKFYALDMFPYPSG
                     AGLHVGHPLGYIATDILSSFKRKQGYNVLHPMGWDAFGLPAEQYAIDTGNDPAEFTAK
                     NIATFKRQMNDLGFSYDWDREVNTTDPKYYKWTQWIFIQLYKRGLAYIDEVPVNWCPA
                     LGTVLANEEVIDGKSERGGHPVERRPMRQWVLRITEYADRLLEDLDELDWPDSLKDMQ
                     RNWIGRSEGAQLTFEIDGTDLSFEAFTTRPDTIFGATYAVLAPEHKLVDKITTAEQRE
                     AVDAYLDKVKTKSDLERTDLAKEKTGVFTGAYAINPASGERMPIWIADYVLASYGTGA
                     IMAVPAHDERDYEFAKEFDLPIVEVVAGGNVSKEAYVGDGEHINSDFLNGLGKDEAIE
                     KAIAWFVENGKGEKKITYRLRDWLFSRQRYWGEPIPIIHWEDGSMTPVDESELPLMLP
                     KTDNIKPSGTGESPLANIEEWVNVVDPDTGMKGRRETNTMPQWAGSCWYYLRYIDPDN
                     DEMLIDPKLAERWLPVDIYVGGAEHAVLHLLYARFWHKVLYDIGVVPTKEPFQKLFNQ
                     GMILGEGHVKMSKSLGNVINPDDIVHSHGADTLRLYEMFMGPLEASKEWSTNGLDGSR
                     RFLDRIWRLFVNEDGTLSSKIGGEPGGALEKVYHQTVKKVTEDLEGMRNNTAISQMMV
                     FINEGYKAESIPKEFVEGFVTLISPITPHLAEELWEKLGHSESISYVAWPTYDESKLF
                     DDTVEVAVQINGKVRTKIDVAKDISKEELEQVALADENVKQWIEGKELKKVIAIPGRL
                     VNIVA"
BASE COUNT         1054 a          773 c         1015 g          873 t
ORIGIN      
        1 agcgatgatt catacgccga gcatctacgt gcaaggtcta ttgcaagcgg aacaggaaaa
       61 acggatagag cgtttaacga ctcgataagg gaaggggatg ttcacgatga atttcagaga
      121 gagaattgca agacgagcgg aaaaggaaat caatgacggc gactatgtga acctaggcat
      181 cggtatgccg acgttggttg ccaactatat atcggacgac aagacggtcg ttctccagtc
      241 tgaaaacgga cttctcggaa tcggtccgta tccgacagag gatcaagtcg atcctgactt
      301 gatcaatgcg ggcaaggaaa cggtgacgac aatcccggga tccgcctttt tcaacagtgc
      361 ggaatcattc gcgatgatcc gtggtggcca tgtcgacgta gcgatcctcg gcggaatgga
      421 agtgtccgaa aaaggcgatt tagccaactg gatgattccg ggcaagatga tcaaagggat
      481 gggcggcgcg atggatctcg tacatggcgc gaagaagatc atcgtcatca tggaccacgt
      541 cgcaaaagac ggctccgcga agatcaaaaa ggaatgctcg ttgccgttga caggaaaagg
      601 cgtcgtccat aagatcatca cggaacgcgc tctgatcgac gtaacgccgg acggcttgga
      661 attgaaggaa gtgttcgaag gcttcaccgt cgaagacgtc atcaattcaa ccgaagcgga
      721 attgaaagtg aatattggat gattgaaata tgccaagtcc tcgtaaatgg acttggcgtt
      781 ttttatctat tggatatgtg gagttatcta ttaaatccgg ctatttatct atcaaataca
      841 ttgacttatc tattaaatat gcggagttat ctatcgaatg tggaaagtta tcaccaaaaa
      901 gggatcttgt actcatgctc ctatcagttg atttcaatca aattgaaagc agtttattac
      961 atggagttgt aaaatggaac aatttcgggt acactacaat aaactgaatt caaatagcgt
     1021 tggcgttgaa gaggaattag taatcggtcc ggtcgattga agagagcagg cggtttggtg
     1081 caagcctgta cgactacgat gaactcgcct ttgagcctgc atcggtgaac gaaaaagtag
     1141 ccagtgccgt tttccgcgtt aaggaaacaa gttgggcggc attgccgtca atttgggtgg
     1201 caccgcggga gtacatatac ctctcgtccc ttatcgatta agggacggga gtttttttat
     1261 ttccgcaaaa cgcgatttga atgtaatggg aaggaatgaa cagagatgag ttacaagcat
     1321 gtggaaatcg aaaagaaatg gcagcaatat tggaaggaca ataagacgta taaaatggtg
     1381 aatgacgatt ccaagccgaa gttctatgcg cttgacatgt tcccgtaccc atccggagcg
     1441 ggacttcacg tcgggcatcc gctcggctat atcgcgacgg acattttaag ttcattcaaa
     1501 cggaagcagg gctataatgt ccttcacccg atgggatggg acgcattcgg tttgccggct
     1561 gaacaatatg ccatcgatac gggcaatgac ccggcggaat tcactgcgaa aaatatcgca
     1621 acgttcaagc gccaaatgaa tgaccttggc ttttcctatg actgggaccg tgaagtgaat
     1681 acgacagacc cgaaatacta caaatggacg caatggattt tcatccaatt gtataagcgg
     1741 ggcttggcgt acatcgatga agtgcctgtc aactggtgtc ctgcacttgg cacagttctt
     1801 gcgaatgaag aagtgatcga cggtaaatca gaacgcggag gacatccggt tgaacgccgt
     1861 ccgatgcgtc aatgggtatt gcgcattacg gaatatgcgg atcgcttgct tgaagatctt
     1921 gatgaacttg attggccgga tagcttgaaa gatatgcaac gcaactggat cggccgctct
     1981 gaaggcgctc aattgacgtt cgaaatcgac ggcacagatc tttcattcga agccttcacg
     2041 acacgtcctg atacgatttt cggtgcgacg tacgcagttc tagctcctga gcataagctg
     2101 gtcgacaaga ttacgacagc ggagcagagg gaagcggttg acgcttattt ggataaagtg
     2161 aaaacgaaaa gcgacttgga acgtaccgat cttgcgaaag agaagacagg cgtattcaca
     2221 ggcgcttatg cgatcaaccc tgcgagtggc gaaaggatgc cgatctggat tgcggattat
     2281 gttcttgcaa gctatggaac tggcgcgatc atggcggttc ctgcacatga cgaacgcgat
     2341 tatgagtttg caaaggaatt cgatttgccg attgttgaag tcgttgcggg tggaaatgtt
     2401 tcaaaggaag cgtatgttgg agacggtgag catatcaact ccgatttctt gaatggacta
     2461 gggaaagacg aagcgatcga aaaggcgatt gcatggttcg ttgaaaacgg caaaggcgag
     2521 aagaagatta cgtatcgtct acgagattgg ctattctcca gacagcgtta ttggggtgaa
     2581 ccgatcccga ttatccattg ggaagatggt tcgatgactc ctgtcgacga atccgaattg
     2641 ccattgatgc tgccgaagac tgacaacatc aagccgagcg gaacgggtga gtctccgctt
     2701 gcgaatatcg aagagtgggt caatgtcgtc gatccggaca caggcatgaa aggccgtcgg
     2761 gaaacgaata caatgccgca atgggcaggc agctgctggt actacttgcg ttatatcgac
     2821 ccggataatg atgaaatgct tatcgatccg aagcttgctg aacgctggct gccggtcgac
     2881 atttacgtcg gcggtgcgga gcatgcggta cttcaccttc tttatgcacg cttctggcac
     2941 aaggtccttt acgatatcgg cgttgtaccg acgaaggaac cattccaaaa attgttcaac
     3001 caaggaatga tccttgggga aggccatgtg aagatgtcca agtcgcttgg caacgtcatc
     3061 aacccggacg acatcgttca ttcgcacggt gcggatacac ttcgtctata cgaaatgttc
     3121 atgggaccgc ttgaagcttc gaaagaatgg tcgacgaacg gtcttgacgg ctcccgtcgt
     3181 ttcctggacc gcatttggcg cctattcgtc aatgaagacg gcacgttaag ctcgaaaatc
     3241 ggcggtgagc caggcggtgc gcttgaaaag gtataccacc aaacagtgaa gaaagtgacg
     3301 gaagacttgg aaggcatgcg caataacact gcgatttcgc agatgatggt cttcatcaat
     3361 gaaggctata aagctgaatc gattccaaaa gaattcgtcg aaggctttgt cacattgatt
     3421 tcaccgatca ctccgcatct agctgaagag ctttgggaga aacttggaca tagtgaatcg
     3481 atttcgtatg tggcatggcc aacgtacgat gaatcgaagc tgtttgatga cacagtcgaa
     3541 gtggctgtac aaatcaacgg aaaagtccgt acgaaaatcg atgttgcaaa agatatttcg
     3601 aaagaagagc ttgagcaggt agctcttgca gacgaaaatg tgaagcaatg gatcgaaggg
     3661 aaagaattga agaaagtcat cgcgattcca ggcagattgg ttaatattgt ggcag
//
LOCUS       DASULI010000346         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1489199,
            whole genome shotgun sequence.
ACCESSION   DASULI010000346 DASULI010000000
VERSION     DASULI010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489199"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFX34_07385"
     CDS             complement(<1..487)
                     /locus_tag="VFX34_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5564765.1"
                     /translation="MRISDFSIKRPVFTIVTMFLVIILGAVSFFKIPVTLIPELNPPV
                     AVVVTNYPGASSTEVSEKLTKPLEASLSTAPGLKSMQSSSQEGSNFILLMFDWSTKID
                     DVQLDIMQRIDQVPVPEGADAPRFMKFDPAQFPVIQLSLRTSSEEADIRDIAERLETD
                     LR"
     gene            complement(602..>2007)
                     /locus_tag="VFX34_07390"
     CDS             complement(602..>2007)
                     /locus_tag="VFX34_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX5564766.1"
                     /translation="WMEIEKQRGISVTSSVLQFDYDGKRVNILDTPGHEDFSEDTYRT
                     LMAVDAAVMMVDSAKGIEPQTIKLFKVCRMRGIPIFTFMNKMDRQGKEPLELMEELEE
                     VLEIESYAMNYPIGMGKEFIGVYDRYNNRIEQARVEGDSHFLPLNEEGELAVEHPMTE
                     TSYYQQALEDVLLLNEAGNEFDVERVAKGELTPVFFGSALTNFGVQTFLETFLQFAPS
                     PQPRKTKDDQLVDPNSEEFSGFIFKIQANMNPAHRDRIAFVRIVSGGFERGMSVTVPR
                     ISKTIKLSQTTQFLADDRETVNEAVAGDIIGLYDTGNYQIGDTVVGGKSNFQFEALPQ
                     FTPELFVRVTAKNVMKSKHFHKGILQLVQEGAIQYYKTLHTEEVLLGAVGQLQFEVFE
                     HRMKNEYNVEVHMEHIGNKVARWIENESDVKESMTGQRSMLVKDRYDNLVFLFENEFA
                     LRWFGDKYPDIRLYSLL"
BASE COUNT          485 a          504 c          459 g          559 t
ORIGIN      
        1 gtctcaggtc tgtttccaga cgctctgcaa tatcccgtat atccgcctct tccgaactcg
       61 tccgaagcga cagctgaatg accggaaact gtgcagggtc gaatttcatg aatctggggg
      121 catccgcgcc ttcaggtacc ggcacctggt caattcgctg catgatgtcc agttgcacat
      181 cgtcaatctt agtggaccaa tcaaacataa gaaggatgaa gttggagcct tcctgtgacg
      241 atgattgcat tgatttcaaa ccgggcgcgg tcgacagact cgcctctagc ggttttgtca
      301 gtttctcact cacttcagtg gaggaggcac ccggataatt ggtgacgacg accgccaccg
      361 gaggattcag ctcaggaatg agtgtcacgg gtattttgaa aaaggaaact gcccctagga
      421 tgatgactaa gaacattgtc acaatcgtaa agacaggtct ttttatggaa aagtcactga
      481 ttctcatttg cacatccctt tcgtgtacca cttacttcta tcatattgaa agacgctagc
      541 aacagcaaac gtgaactata taaaaacccg ggagaccaag tcccccggtt agcttgataa
      601 attataatag gctgtacagt cggatgtccg gatatttgtc cccgaaccaa cggagtgcaa
      661 actcgttttc gaaaaggaag acgagattat catatcgatc cttcacgagc atggatcgtt
      721 gacctgtcat cgactctttc acatcgcttt cattttcaat ccagcgggcg actttgttgc
      781 cgatatgctc catatggact tccacgttgt attcgttttt catacggtgc tcgaacactt
      841 caaattgaag ttgaccgacc gcgcctaata agacttcttc cgtatgcagt gttttgtaat
      901 attggatggc accttcctgc actaattgaa gaatcccttt atggaaatgc tttgatttca
      961 tgacgttttt cgctgtgaca cggacaaaca attcaggcgt gaactgtggc aatgcttcaa
     1021 attggaaatt cgatttccct ccaacaactg tgtcgccgat ttgatagttc cccgtatcgt
     1081 acaagccgat gatatcgcct gcaactgcct cattcaccgt ttcacggtca tccgccaaaa
     1141 attgcgtcgt ctgcgataat ttgatcgtct tcgaaattcg aggcacggta accgacattc
     1201 cacgttcaaa tccgccggaa acgatccgga cgaaagcgat acggtcacgg tgggccgggt
     1261 tcatattcgc ttggattttg aaaatgaatc ccgagaactc ttctgaattc ggatcgacga
     1321 gctgatcgtc ctttgttttc cttggctggg gtgaaggtgc gaactgaagg aaagtctcca
     1381 aaaatgtctg cacgccaaaa ttcgtcaaag cgctgccgaa aaatacaggc gtcaattccc
     1441 ctttggctac ccgttccacg tcaaactcat tgccggcttc gttcaaaagc aagacgtcct
     1501 caagcgcctg ctggtaatag gaagtttcag tcatcggatg ctcgaccgcc agttcccctt
     1561 cttcattcaa cggaaggaag tggctgtcgc cttctacgcg tgcttgttcg atgcgattgt
     1621 tgtaacggtc atagacaccg atgaattcct tgcccatgcc gatcgggtaa ttcattgcat
     1681 atgattcgat ttcgagcact tcttccaatt cttccatcag ttcgagcggc tctttccctt
     1741 ggcggtccat tttgttcatg aatgtgaaga tcgggatgcc tctcatccgg cagactttga
     1801 atagcttgat tgtctgaggc tcgatccctt ttgccgaatc gaccatcatg acagcagcat
     1861 cgaccgccat cagcgtgcga taggtgtctt cactgaagtc ttcgtgtccc ggtgtatcca
     1921 gtatattgac gcgcttgccg tcgtagtcga attgcaatac tgaggaggtt acggatattc
     1981 cacgttgttt ttcgatttcc atccagt
//
LOCUS       DASULI010000347         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_661002, whole
            genome shotgun sequence.
ACCESSION   DASULI010000347 DASULI010000000
VERSION     DASULI010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661002"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFX34_07395"
     CDS             <1..509
                     /locus_tag="VFX34_07395"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5564767.1"
                     /translation="LGGLQSMNSLRYAGLNSVYLPDGLQSAVSASAATSRTVGTGEND
                     FSDFIRRAAETYRLPENLIASVIRQESNFNSAAVSGAGAMGLMQLMPGTARLLGVRDG
                     FDPEQNIMGGAKYLRQMLDQFGNIETALAAYNAGPGNVRKYGGIPPFKETEQYVSKVL
                     NYYNRYEI"
     gene            604..996
                     /locus_tag="VFX34_07400"
     CDS             604..996
                     /locus_tag="VFX34_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEX5564768.1"
                     /translation="MTRKPLLPYEEIGAEKLSELIDRFYEKVAVHPDLYPIFPEDLTE
                     TARKQKQFQTQYLGGPNLFTEEHGHPMMKARHMPFPITPARAKAWLACMAEAMDDVGL
                     EGRFREVYYHRLAMTANHMVNRSEGDER"
     gene            993..1799
                     /locus_tag="VFX34_07405"
     CDS             993..1799
                     /locus_tag="VFX34_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5564769.1"
                     /translation="MNRETLLESSATTSASLSKPIEIYVFVDPLCMNSWALQPLLRRL
                     QVEYERYFTLKIVLRTSLSKTNACCFVNMDDAKACDKSHPAFPSIALKAAEFQGKRAA
                     FRFLSKLFEYTYLKKRNVLSFSVLVEITESLGLDVDEFILDFSSHHVLRSLQVDLHLA
                     NEMEVEDAPSFVFFNENIEDEGLKVNGLHSYEIYEQILEEMVGYEITPDAPPTLDELF
                     QRFDSLSTLEVAEIYNIPEKSAERELKKQLLLQKIERLPLSNMTLWRKRH"
     gene            complement(2508..>3544)
                     /locus_tag="VFX34_07410"
     CDS             complement(2508..>3544)
                     /locus_tag="VFX34_07410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5564770.1"
                     /translation="EAALSNNHIPEQVYDNLVNTINKNLELHHRYVALRKKVLGLDEL
                     HMWDMFAPMVKDVDMKIPYEEATETMLESFHPLGEEYTSIVKEGLENRWVDVRENKGK
                     RSGAYSSGSYGTNPYILMNWQDNVNNMFTLAHEFGHSVHSYYSRKNQPYNYSGYSIFV
                     AEVASTVNEAILNDHLLKTIDDKQKRIYLLNHWLEGFRGTVFRQTMFAEFEHLIHQLD
                     QQGVALTADKLSEEYAALNKKYFGDAVEEDKEIALEWARIPHFYYNYYVYQYATGFSA
                     AVALSHQILTEGQPAVDRYINHFLKAGSSDYPIEVLKKAGVDMTSTQPIEEACKVFEE
                     RLNELEELLG"
BASE COUNT          974 a          804 c          795 g          971 t
ORIGIN      
        1 tgttgggtgg acttcaatcg atgaatagcc ttcgctatgc cggccttaac agtgtttatt
       61 tgccggatgg ccttcaatct gcagtttccg cttcggccgc aacgagtcgg accgtcggga
      121 caggggaaaa tgatttcagc gatttcattc gccgtgctgc ggaaacatac cgcttgcctg
      181 aaaacctgat cgcatccgtc atcagacagg aatccaactt caatagcgcc gcggtgagtg
      241 gtgcgggtgc aatgggcttg atgcagctca tgcctggcac agcaagattg ttgggtgtga
      301 gggatggatt cgaccccgag cagaacatta tgggcggagc gaagtatttg cggcagatgc
      361 ttgaccagtt cgggaatatc gaaaccgcat tggccgcgta taatgcaggt cctggaaacg
      421 taaggaaata cggcggaata ccgcctttca aggaaacaga acaatatgtt tccaaagtgc
      481 taaattacta taatcgttat gaaatctgac aacatttcaa ttcgcttgaa ggcaatttgt
      541 ttgtgaaaga attgtttgct tgttaaaatg tccatacagt cagaacaaag gagattgaca
      601 tacatgactc gaaaaccttt gctcccttat gaggaaatcg gtgctgaaaa attatcggaa
      661 ctcatcgatc gtttttatga aaaagtggct gtccacccgg acttatatcc gattttccct
      721 gaagatctga cggaaacagc acgaaagcag aaacaatttc aaacacaata tttaggcgga
      781 ccgaatttat tcacggaaga gcacggccat ccgatgatga aagcgcgtca tatgcctttt
      841 ccaataacac ctgcacgggc aaaagcatgg ctcgcctgta tggctgaagc gatggatgat
      901 gtaggccttg aaggacgctt ccgtgaagtg tattatcata ggctcgccat gactgcgaac
      961 catatggtga atcgaagcga gggggatgag cggtgaaccg ggaaactctg ttggaaagca
     1021 gcgcgactac ttcagcctcc ttgtcaaagc cgatcgaaat ttacgtattc gtagatccct
     1081 tatgcatgaa cagttgggca ctccagccgt tgcttaggag attgcaagtc gaatacgaac
     1141 gttatttcac attgaagatt gtccttcgta cctctttgtc gaagacgaac gcatgctgtt
     1201 ttgtcaatat ggatgatgcg aaggcttgcg ataaaagcca tcctgccttt cctagcatcg
     1261 ctttgaaagc ggcggaattc caagggaagc gagcagcctt ccggttcctt tccaagctgt
     1321 ttgaatacac ctatttgaaa aagaggaacg tcctctcctt ttcagtgctt gttgaaataa
     1381 ccgaaagcct tggattggat gtcgatgaat tcatcctcga tttctcatcg catcatgttt
     1441 tacgttcttt acaggtcgat ttgcatttag cgaatgaaat ggaagtggaa gacgcacctt
     1501 ctttcgtttt cttcaatgaa aatattgaag atgaagggtt gaaggtgaac ggattgcatt
     1561 cctatgaaat atatgagcag attttggaag agatggtcgg gtatgaaatt acaccagatg
     1621 ccccgcctac gttggatgaa ctattccagc gtttcgattc tctttctaca ttggaggtcg
     1681 ccgagattta caacatcccg gaaaaatccg cagaacgcga actaaaaaag cagctccttc
     1741 ttcaaaagat cgaaaggctg ccgctatcga atatgacatt atggcgcaag cgtcattgaa
     1801 gtaataaagc agattgcatg gtgatcgaat ccatgcaatc tgctttttaa attttattca
     1861 taggctgttg ctttccattc cagcactcgc tttccgcggg cgttgcctta gccaatcgaa
     1921 ccgcgaagag ttcgattcgc cttaatttct gcgcctattg cagaaattaa ggcatcctaa
     1981 ttatgctcgc aacgctgcac ttgtgctcgc aaacgccgtt cttcgtaaca gcgttcgcag
     2041 gagtctcgca cttgcactcc agcaaccgca gctacttata tagaaattta ttaataaggc
     2101 ccccctgagt aagctcaaac aatacgcaag gatatctaaa gttaaagcaa aagaaaagag
     2161 catccgctct tgggggagag ggatgctctt ttacaaaggg aatgggagaa atgttcacgt
     2221 tcaaacaaaa ggggtgtatg tttggtatgt gatttatttc acatctataa cttatcataa
     2281 aataatagta acatcaacat ttcacatgaa gtttcacatc tctgtcacaa ataaaacacc
     2341 aaacaaacgc cttacctaaa gaactccatt gagttggtaa atcgatccag actcgaatga
     2401 tttaaatgta agcgctttac tcattattat ataaatataa tcaagaatcg ccaaaccaaa
     2461 aaaacctgac ttaagcatcg aatatgctaa gtcaggtcca tttttactta tccaagtaat
     2521 tcttccagct cattcaatct ctcctcgaac actttacacg cctcttcgat cggctgtgtg
     2581 ctcgtcatat cgacgcctgc ctttttcaga acttcgatcg gataatcgga agatccggct
     2641 ttcaggaaat gattgatgta acggtcgacg gcaggctgcc cttccgtcaa gatctgatgg
     2701 ctgagcgcca ctgcggcact aaagcccgtt gcatattggt aaacgtagta attgtagtag
     2761 aaatgtggga ttcgcgccca ttcgagagcg atctccttat cctcttccac tgcgtcacca
     2821 aagtatttct tgttcaacgc ggcatattcc tcagacaatt tatccgcagt caatgcaacg
     2881 ccttgctggt caagctgatg gatcagatgc tcgaactccg caaacattgt ctggcggaag
     2941 accgtgccac ggaatccttc tagccagtgg ttcaataaat aaatgcgttt ttgcttatcg
     3001 tcgatcgttt tcaataaatg atcgttcaaa atcgcttcgt tcacggtaga tgcgacttca
     3061 gcgacgaaaa tggaatatcc gctgtagttg tacggctgat tcttccttga atagtagctg
     3121 tggacgctat ggccgaactc gtgcgccaat gtgaacatat tgttgacgtt gtcttgccag
     3181 ttcatgagga tgtatggatt cgtgccatag gatcccgatg aataagcgcc agaacgcttc
     3241 cctttgtttt cacgcacgtc cacccaacgg ttttccagcc cttctttgac gattgatgtg
     3301 tattcctcgc caagcggatg gaaactttcg agcatcgttt cagtcgcttc ctcataagga
     3361 atcttcatgt ccacgtcttt aaccatcggc gcaaacatat cccacatatg gagttcatcc
     3421 aggccaagta cctttttacg caacgcaaca tagcggtgat gcagttcaag atttttattg
     3481 attgtattga caaggttgtc atacacctgc tccggaatat ggttgtttga caaagcagct
     3541 tccc
//
LOCUS       DASULI010000348         6070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1210498,
            whole genome shotgun sequence.
ACCESSION   DASULI010000348 DASULI010000000
VERSION     DASULI010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210498"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFX34_07415"
     CDS             <1..308
                     /locus_tag="VFX34_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009335912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564771.1"
                     /translation="VVLSAFIEFVVGYLLVVGILNRLLAVVVTVLFILTTTIFGYTEV
                     VGHGMIHIVLLLFIIEGISFYHPPVKMHETRLDQMIFVFLNFLFVLSTFLLIYYRFA"
     gene            complement(372..1523)
                     /locus_tag="VFX34_07420"
     CDS             complement(372..1523)
                     /locus_tag="VFX34_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX5564772.1"
                     /translation="MQNERKRILTMLENGTISMDEALTLLEAMEKKQTSTGFSQAEEK
                     KTFTEEPKSEDFSGNATGKDSSMDDFIEDIRKDFVTVGDRFMQFMQTTVERMKGFDFD
                     TPFGGGSTFHHTIVKEADGLDEILVDVDHGSVSIHATDNPEVRAEFTVKSFNGRSEED
                     AKKEFLDKLIFLKDEGKLRYLSDLKLSQVNIDLFIPKQDYRKVSVRLLNGHFNLKDIA
                     VEKLYTKTANGKVDVERIEFKEAELEAGNGSIRLNESTGDMLEAETINGRVYVSGKLK
                     DVEAKSLNGHVVVTTTDEAAQKIDAKTVSGSVELYIPSDVALRGEISTNMGRLDLQLN
                     DVSKTTEQEQFLHRTIIFNKDKEGEAAPLHVHGEAKTGSVIVCYNVKHD"
     gene            complement(1642..4512)
                     /gene="uvrA"
                     /locus_tag="VFX34_07425"
     CDS             complement(1642..4512)
                     /gene="uvrA"
                     /locus_tag="VFX34_07425"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5564773.1"
                     /translation="MKNTEIVIQGARVHNLKDIDVRIPRDKLVVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDVIEGLSPAISIDQKTTSRNPRSTVGT
                     VTEIYDYLRLLYARVGKPICPNHGIEISSQTVEQMVDRIMQLPERSRLQILAPVISGR
                     KGTHAKLIEDIKKQGYVRIRVNGDVMDLDDNIELNKNKKHTIEVVIDRIVVKEGIEAR
                     LSDSLESALRLAEGTVLVDVIDVEELLFSEHHACPMCGFSIGELEPRMFSFNSPFGAC
                     PDCDGLGTKLEVDPDLVIPDTSLSLNENAIAPWIPTSSQYYPELLKTICKHFKIPMDV
                     PVSDLPDEDLNIILYGSRDQKIKFQYTNEFGNTRVNNIYFEGVLANVERRFKETSSDY
                     IREQMEKYMASRACPTCHGYRLKEESLAVKVNGTHIGEVTALSITEADRFFSNLVLSE
                     KDMQIARLILREIAERLGFLINVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSRLTGV
                     LYILDEPSIGLHQRDNDRLIGTLQSMRDIGNTLIVVEHDEDTMIAADYLIDIGPGAGA
                     AGGQIVSAGTPKEVMNDPKSLTGQYLSGRKFIPLPIERRQSDGRVVSIKGASENNLKN
                     VDADFPLGQFIAVTGVSGSGKSTLVNEVLYKVLAQKLNRSKQKPGQFKSVTGLEELEK
                     VIDIDQSPIGRTPRSNPATYTGVFDDVRDVFAMTNEAKVRGYKKGRFSFNVKGGRCEA
                     CRGDGIIKIEMHFLPDVYVPCEVCHGKRYNRETLEVTYKGKNIADVLSMTVEDALVFF
                     ENIPKISRKLQTIVDVGLGYVQLGQPATTLSGGEAQRVKLASELHRRSNGKSFYILDE
                     PTTGLHVHDIEKLLLVLQRLVDTGNTVLVIEHNLDVIKTVDHIIDLGPEGGDKGGQII
                     ATGTPEQVAESEVSYTGHYLKPILERDRQRMKERIEAAAGKE"
     gene            complement(4520..>6070)
                     /gene="uvrB"
                     /locus_tag="VFX34_07430"
     CDS             complement(4520..>6070)
                     /gene="uvrB"
                     /locus_tag="VFX34_07430"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5564774.1"
                     /translation="IYGLGSPEEYGKHVVSLRTGMEIGRNELLRRFVDIQYSRNDINF
                     TRGTFRVRGDVVEIFPASQDENCIRIEFFGDEIDRIREVDALTGEILGDREHVAIFPA
                     SHFVTGEEKMVKAIANIEAELEEQLAILRKEDKLLEAHRLEQRTRYDLEMMREMGFCS
                     GIENYSRHLTLRPAGATPYTLLDYFPDDFLIVIDESHVTLPQIRGMYNGDQARKQVLV
                     DHGFRLPSALDNRPLMFEEFERYVHEAIYVSATPGPYEIEHTPEMVEQIIRPTGLLDP
                     IIDVRPIEGQIDDLIDEINERTNRNERVLITTLTKKMSEDLTDYLKDIGIKVQYLHSD
                     VKTLERIEIIRELRLGKYDVLIGINLLREGLDIPEVSLVAILDADKEGFLRSERALIQ
                     TIGRAARNSEGKVIMYADRYTDSMKKAIDETARRREIQKAYNEKHGITPTTIKKEVRD
                     VIRATQVAEEEISALEKVTKGKKLSKDDKVKLLATLEKEMKEAAKALDFERAAELRDT
                     ILELKAEG"
BASE COUNT         1344 a         1612 c         1335 g         1779 t
ORIGIN      
        1 ttgttgtgct ctcagcattt attgaattcg tcgttggtta cttattagtc gttgggattc
       61 tcaaccgttt attagccgtt gtcgtaactg ttttatttat cctcacaacg accatctttg
      121 gctacaccga agtagtagga catgggatga ttcatattgt tttactgtta tttattatcg
      181 aaggaatatc cttttatcat ccacctgtta aaatgcatga aacccgattg gaccaaatga
      241 tatttgtttt cctgaacttt ttatttgtgc tcagtacatt tttattgatc tattaccgat
      301 ttgcataaaa cacaaaaaag actgccggag aaaatcaatt ctcactgatt ttcttggcag
      361 tcttttttcc gttaatcgtg ctttacgtta taacaaacaa tgacagagcc cgttttcgct
      421 tctccatgaa cgtggagcgg cgcggcttcg ccttctttgt ctttgttgaa aatgatcgtg
      481 cggtgcagga actgttcctg ttccgtcgtt ttgctgacat cgttcagctg aagatccaaa
      541 cgtcccatgt tcgttgagat ttcacctcga agtgcaacgt cggatggaat atacagctca
      601 acggaaccgc tcaccgtttt cgcatcgatt ttttgtgcgg cttcatctgt cgtcgttaca
      661 acgacatgac cattcaacga tttcgcttcg acgtccttca acttgccgga cacgtagaca
      721 cggccgttga ttgtctcggc ttccaacatg tccccagtgc tttcattcaa acggatcgat
      781 ccgttgcccg cttccagctc ggcctctttg aactcaatcc gctcgacatc cacttttcca
      841 ttcgctgttt tcgtataaag tttctcaacc gcaatatcct tcaagttgaa atgcccattc
      901 agaagtcgta cggaaacttt gcgataatcc tgtttcggga tgaaaagatc gatattcacc
      961 tggcttaatt tcagatcgct taaatagcgc agtttgcctt cgtctttcag gaaaatcaat
     1021 ttatcgagaa attccttttt cgcatcttct tccgatcttc cattaaacga cttcacggtg
     1081 aattccgcgc gaacttcagg attatccgtc gcatggatcg aaacgctgcc gtgatcgaca
     1141 tcgacgagaa tttcatccaa tccgtccgct tccttcacaa ttgtatgatg gaatgtggag
     1201 ccgccgccaa atggtgtgtc gaaatcgaat cctttcattc tctccacagt tgtttgcatg
     1261 aactgcatga aacgatcgcc gacggttacg aaatccttcc ggatatcttc aatgaagtca
     1321 tccatcgatg aatctttccc tgtcgcgttt ccggagaagt cctcgctttt aggctcttcc
     1381 gtgaaggttt tcttctcctc cgcctgggaa aatccagtcg aggtttgttt cttctccatc
     1441 gcttccaaaa gggtcaacgc ttcgtccata gagattgtgc cgttttccaa cattgtcaag
     1501 atacgtttac gttcattctg catctttccg cctcctaggt atgcttgaga ttttcttttc
     1561 ggatatgagt ccccgctatt tcaaaaccat tggagacttc ctcaacacta tctatatata
     1621 cgaatcatct atgagaaggt ttcattcttt tccggctgcc gcttcaatcc gttctttcat
     1681 tctttgtcgg tcgcgttcca gtatcggctt caaatagtgg cctgtgtagg agacttcact
     1741 ttccgccact tgctccggag tacccgtcgc gatgatctgg ccgcctttgt ccccgccttc
     1801 aggtccgaga tcgataatat ggtcgaccgt tttgatgacg tccagattat gttcgatgac
     1861 aaggacggta ttccctgtat caacgagccg ctgcaaaacg agaagcagct tttcaatatc
     1921 gtggacatgc agccccgttg tcggttcgtc caatatatag aaggatttcc cgttggatcg
     1981 tctatgcaac tccgatgcaa gcttcaccct ctgcgcttct cctcccgaca acgtcgtggc
     2041 cggctgtccg agctggacgt agccgagccc gacgtcaaca atcgtttgca gttttctgct
     2101 gatcttcggg atattttcga agaaaacgag agcgtcttcg acggtcatgg aaaggacatc
     2161 tgcaatattc ttccctttat acgtcacttc aagcgtttca cggttatatc ttttcccgtg
     2221 gcacacttca catgggacat acacatcagg aaggaaatgc atctcgatct tgatgattcc
     2281 gtcaccgcgg caggcttcac agcggccgcc ttttacgttg aaactgaaac ggcctttttt
     2341 gtagcctctc actttcgcct cgttcgtcat ggcaaagaca tcccggacat cgtcaaagac
     2401 tcctgtatag gtcgccgggt tcgatcttgg cgtccgcccg atcggggatt ggtcgatatc
     2461 gatgactttt tccaattcct caaggcctgt tacggacttg aattgtccag gtttctgctt
     2521 cgaacggttc aatttttggg cgagtacttt gtaaagcact tcattcacaa gtgtactctt
     2581 gccggagccc gaaacgccag tcaccgcgat gaactgtcct aacgggaagt ccgcatcgac
     2641 atttttcaag ttgttctcag acgcgccttt gatcgatacg accctgccat cggactgcct
     2701 ccgttcgatt ggcaaaggaa tgaacttcct gccgctcaaa tattggccgg tcaatgattt
     2761 cggatcgttc atcacttctt tcggagttcc tgcggatacg atttgccctc ctgcagcccc
     2821 agctcctggt ccgatatcaa tcaaataatc cgctgcaatc atcgtgtctt catcatgctc
     2881 gacgacaatg agcgtattcc cgatatcccg catgctttgc agcgtgccga tgagacggtc
     2941 gttgtcccgc tgatggagac cgatggacgg ttcatccaaa atatagagga cgccggtcaa
     3001 tctggagccg atctgggtcg caagccggat gcgttgcgct tcaccgccgg ataatgtgcc
     3061 ggcagccctt gataacgtca agtaatccag cccgacattg atgaggaatc ctagtctctc
     3121 tgcaatttcc cgcaagatca gccttgcaat ctgcatatcc ttttcggaaa ggacgaggtt
     3181 gctgaagaaa cgatccgctt ccgtaatgga caatgcagtc acttcaccga tatgcgtgcc
     3241 attcactttg actgccaatg attcctcttt caatcggtac ccatgacaag ttggacaagc
     3301 ccggctcgcc atatatttct ccatttgttc acggatataa tcggaggaag tctccttgaa
     3361 acgtcgctcc acatttgcga gcacaccttc gaaatagatg ttgttcaccc gtgtatttcc
     3421 gaattcattc gtatattgga atttgatttt ttggtccctc gaaccataga ggatgatgtt
     3481 cagatcctca tcgggcaaat cgctcaccgg tacatccatc ggtattttga aatgcttgca
     3541 gatcgtcttc aacagctccg ggtagtattg ggagctcgtc ggaatccatg gtgcaatcgc
     3601 attttcattc aaagagaggg atgtgtcagg gatgacgaga tcggggtcga cctcaagctt
     3661 cgtccccaat ccatcgcaat ccgggcaagc cccgaatgga ctgttaaatg aaaacatgcg
     3721 cggctcaagt tccccgatgg aaaatccgca catcggacat gcatgatgct cgctgaacag
     3781 tagttcttcg acatcgatca cgtcgaccag tacagtccct tccgccaaac ggagggcgga
     3841 ttcgagtgaa tcgctaagtc tcgcttcgat tccctctttc acgacaatgc ggtcgatgac
     3901 aacttctatc gtatgctttt tatttttatt cagttcaata ttgtcatcaa ggtccattac
     3961 gtctccatta acacggatcc gtacatatcc ttgtttttta atgtcctcga tcagcttcgc
     4021 atgcgtccct tttctgcctg aaatgaccgg cgccaagatt tgaagccttg atcgttcagg
     4081 caactgcatg atccggtcga ccatctgctc gaccgtctgg gaggagattt caatcccgtg
     4141 attcggacag atcggcttgc cgacccgtgc atacagcagt cttaagtaat catagatttc
     4201 agttactgtt ccgacagtgg aacgcggatt gcggcttgtc gtcttctggt cgatggaaat
     4261 cgcaggcgac agcccctcga tcacgtccac atcaggcttg tccatctggc ctaggaactg
     4321 cctcgcataa gcggaaagcg attcgacata ccgtctttgc ccttccgcat aaatcgtatc
     4381 gaatgcaagg gacgatttcc ccgaacccga caagccggtc atgacgacga gcttgtcgcg
     4441 gggaatacgg acatcaatat ccttcagatt atgcaccctt gccccttgaa tgactatttc
     4501 cgtgtttttc atcgcatttt cacccttcag ccttcaattc taatatcgta tcgcgcaatt
     4561 cggcagccct ttcgaaatca agcgcttttg ccgcctcctt catttccttc tccagcgtag
     4621 caagcaattt cactttgtcg tctttcgaca gcttcttgcc tttcgtcact ttttccaatg
     4681 ccgatatttc ctcttcggct acttgggtcg ctctgatgac atcgcgcact tcttttttga
     4741 ttgtcgtcgg tgtgatacca tgcttttcgt tgtatgcctt ttggatttcc cggcgcctcg
     4801 ctgtttcatc gatcgccttt ttcatcgaat ctgtgtaacg gtccgcatac atgatgacct
     4861 ttccttcgga gttacgcgct gcccggccga tcgtctggat gagggcacgt tcggaacgga
     4921 ggaacccttc cttatccgca tccaatatgg cgacgagcga cacttcggga atatcgagcc
     4981 cttccctcag caagttgatg ccgatgagta catcgtactt gcccaaacgg agttcacgga
     5041 taatttcaat tcgctctagc gttttgacat ccgaatgcaa gtattggact ttgatgccga
     5101 tatccttcaa ataatcggta aggtcttcgg acatcttttt cgtcaatgta gtaatcaata
     5161 ccctttcgtt ccggtttgta cgctcattga tttcatcgat caaatcatcg atctggccct
     5221 cgatcgggcg cacgtcaatg atcggatcaa gcaaaccggt cggccggatg atctgttcga
     5281 ccatctcagg cgtatgctcg atttcgtatg gaccgggtgt tgcggatacg tagatcgctt
     5341 cgtggacgta ccgctcgaat tcttcgaaca tgagcggcct gttgtccaag gcggaaggca
     5401 gacggaagcc atgatccacc agaacttgct tcctggcctg gtcgccgtta tacatgccgc
     5461 ggatctgcgg caacgtgaca tggctttcat cgatgacaat caggaaatca tccgggaagt
     5521 agtcaagaag cgtatagggg gtcgcccctg ccggccttaa tgtcaggtgc ctcgaataat
     5581 tctcgattcc tgagcagaac cccatttccc gcatcatttc caaatcataa cgcgtgcgct
     5641 gctcgagccg gtgcgcttcc aaaagcttat cttccttgcg caatatggcg agttgttctt
     5701 ccaattcagc ttcgatattc gcaatggcct tcaccatttt ctcttcgcct gtgacgaagt
     5761 gggatgccgg gaagatggcg acatgttcgc gatcgcctaa tatttcccct gtcagtgcgt
     5821 ccacttcacg gatgcgatcg atttcatcgc cgaaaaattc gatgcggatg cagttttcat
     5881 cctgtgatgc agggaagatt tcaacgacat cccctctcac ccggaatgta ccccgtgtga
     5941 aattgatgtc atttcttgag tattgaatat cgacgaagcg acgaagtagt tcattcctgc
     6001 cgatttccat acctgtccgg agcgagacga catgctttcc gtattcctcc ggtgaaccta
     6061 ggccgtaaat
//
LOCUS       DASULI010000349         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1761614,
            whole genome shotgun sequence.
ACCESSION   DASULI010000349 DASULI010000000
VERSION     DASULI010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761614"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFX34_07435"
     CDS             <1..639
                     /locus_tag="VFX34_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5564775.1"
                     /translation="AFKAAAEKLGAEVVLEEFADPAATDFTSNLQKIVEAKPDYLFVV
                     WAGANSPWNQIADMKLQEKGIKISTGAPDIAALSIMEPLVGMEGFTVYYHELPNNKVN
                     DWLVEKHKEKFNGEVPDLFTPGGMSAAIAIVEALKKSNGDADGNKLIDVMEGMSFETP
                     KGTMTFRPDDHQALQTLYSIRLEKKDGVPYPVPVLIRELSPEETEPPIMNKK"
     gene            736..1509
                     /locus_tag="VFX34_07440"
     CDS             736..1509
                     /locus_tag="VFX34_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564776.1"
                     /translation="MEPIIRTENLTIQFGGHTAVDHVNFELPAKQFKSIIGPNGAGKT
                     TFFNLISGELKATSGSVYYKGEKIDRLSSVERTRRGIGRSFQITNVFPNLPVKENVRL
                     AIQSREKVRFNLFRHVTKYKELEYEVHNLLSLVLLDGKEEALASQLSHGEKRKLEIAM
                     LLALDTDVLLLDEPTAGMSLEEVPAILDVIRQIKEKGEKTILLIEHKMDMILDLSDSI
                     MVLFNGKLIADGTPEEIMENEMVQHAYLGGLYDDNIAAG"
     gene            1487..2194
                     /locus_tag="VFX34_07445"
     CDS             1487..2194
                     /locus_tag="VFX34_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564777.1"
                     /translation="MTTLLRVEGIHTFIGQYHILQGVSFEAKEGEVTVLLGRNGAGKT
                     TTLKTVMGLTPASKGSITYLGANITKSPTHSIANAGIGYVPEDQGIFGTLTVGENMKV
                     AMRKEDDAAYERQEYVLNLFPDLKKFWKKEGAYLSGGQKQMLAMARAFVNDSKVLLID
                     EPSKGLAPIVIGHVMEAITEMKKRTTIVLVEQNFMMASRIGDTYTIIDHGETVHTGRI
                     SDLTEDEQLKRSYLGIG"
     gene            2207..>2300
                     /locus_tag="VFX34_07450"
     CDS             2207..>2300
                     /locus_tag="VFX34_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5564778.1"
                     /translation="MELILNLTINGLATGMLIFLLAAGLTLIFGL"
BASE COUNT          702 a          457 c          589 g          552 t
ORIGIN      
        1 gcatttaaag ctgcggcaga aaagcttggt gcggaagtag tcctggaaga atttgcagac
       61 cctgcagcaa ctgactttac gtctaacttg caaaagattg tcgaggcaaa accagattac
      121 ctattcgtgg tatgggctgg ggcgaactct ccatggaacc agatcgccga catgaagctg
      181 caagagaaag gcattaagat ttcaactggg gcacctgaca ttgctgcgct ttccatcatg
      241 gagccgttag tcggtatgga aggattcaca gtgtattatc acgaactgcc aaataacaaa
      301 gtaaacgatt ggttagtcga gaagcacaaa gagaaattca acggggaagt tcctgatcta
      361 ttcacgccag gtggaatgtc agcagcgatt gccatcgtgg aagcattaaa gaagtcgaat
      421 ggtgatgcag acggcaacaa gctgattgat gtgatggaag gcatgtcatt tgaaacgcca
      481 aaaggaacaa tgacattccg tccggatgat caccaggcgt tgcagacgct gtattccatc
      541 cgtttggaaa agaaggatgg cgtcccttat ccggttccag ttctcatccg ggagctttca
      601 ccggaagaga ccgagccgcc aatcatgaat aagaaataag aattgagagc atggccacat
      661 acggcctgtc tctctccagg gtatccgtcg taacggagtt ctggagaggg ataggagagg
      721 aaaggggaga aggcaatgga gccaatcatt cgaacagaaa atttaacgat tcagtttggc
      781 gggcataccg ctgtcgatca cgtaaatttt gaactacctg caaaacagtt caagtccatc
      841 attggaccga acggagcggg aaagacaaca ttcttcaatt tgattagcgg ggaattaaaa
      901 gcgacatcag gcagtgtgta ctataaaggc gagaaaattg atcgcctttc ttctgttgag
      961 cgtacacgga gaggaattgg ccgctccttt caaattacaa atgtgtttcc gaatctacct
     1021 gtgaaagaaa atgtccgtct cgccattcag tcacgcgaaa aagttcggtt taacttgttt
     1081 cgtcacgtta caaaatacaa ggaacttgaa tatgaagttc ataacttatt gtccctcgtt
     1141 ttattggacg gaaaagaaga agcgcttgca agccagctgt cacacggcga gaagaggaag
     1201 cttgaaatcg ccatgcttct tgcccttgac acggatgtac tgcttcttga tgagccgaca
     1261 gcaggaatgt cactggaaga ggtccctgca attcttgatg tcatccgtca aataaaagag
     1321 aaaggcgaga agacgatttt attaattgag cacaagatgg atatgattct tgatctttcc
     1381 gattccatta tggtgctgtt caacggcaaa ctgattgcag acggaacacc ggaagaaatt
     1441 atggaaaatg aaatggttca gcatgcctat ttgggaggac tttacgatga caacattgct
     1501 gcgggttgaa gggatccaca cattcatcgg ccagtatcac atcctccaag gtgtttcatt
     1561 tgaagcgaag gaaggggaag taactgttct tttaggacgt aacggagcgg gtaaaacgac
     1621 aactttgaag acagtcatgg gattaacacc cgcatctaaa ggatcgatta cgtacttggg
     1681 tgccaacatt acaaagtcgc cgacccattc cattgcgaat gcaggaatcg gctatgtacc
     1741 ggaggaccaa ggtatcttcg gtacgttaac tgttggagag aacatgaaag ttgccatgcg
     1801 aaaagaagac gatgcagcct atgaacggca ggaatatgtg ttgaacttat ttccggattt
     1861 gaagaagttc tggaaaaagg aaggggcgta tttatccgga ggacagaaac agatgcttgc
     1921 gatggcacgt gcatttgtca atgacagtaa agtgcttctg attgacgaac cttcaaaagg
     1981 gctggctcca atcgtaatcg gtcatgtcat ggaagcgatt acggaaatga aaaaacgcac
     2041 gacaattgtt ctagttgaac agaacttcat gatggcgagc cgaattgggg atacgtatac
     2101 gattattgac cacggggaaa cagtccatac cggaagaatt tctgatctga cagaagatga
     2161 gcaactaaaa cggtcctact tgggcatcgg ataaggaggc agaaagatgg aattgattct
     2221 taatttaaca attaacggac tagcgaccgg aatgctcatc tttttgctgg cagcaggatt
     2281 aacattaatc ttcggtttga
//
LOCUS       DASULI010000350         3522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_478495, whole
            genome shotgun sequence.
ACCESSION   DASULI010000350 DASULI010000000
VERSION     DASULI010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478495"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VFX34_07455"
     CDS             <1..147
                     /locus_tag="VFX34_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5564779.1"
                     /translation="FAKEKLMNEALPSRYRFIFYHDAVYRLVQWDEGGKEITESVERT
                     RSLT"
     gene            complement(216..530)
                     /locus_tag="VFX34_07460"
     CDS             complement(216..530)
                     /locus_tag="VFX34_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564780.1"
                     /translation="MKVKDFVAGVLTGVAAGLVVNQAIGKMNPNTPAEHVLDAVKDAF
                     KKEGPIDGSWIVMKPEPFTNNVLSMEVYRGGITRIRNGELEQFEFAANAQTGTVVELI
                     QK"
     gene            716..1789
                     /locus_tag="VFX34_07465"
     CDS             716..1789
                     /locus_tag="VFX34_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX5564781.1"
                     /translation="MNNETLTMFKTLTELPGAPGNEHLVRNYMREELGKYSDEIIQDN
                     LGGVFGVRKGPENGPRIMVAGHMDEVGFMVTAITDNGMLRFQPLGGWWSQVLLAQRVQ
                     IITDEGPVVGVIGSIPPHLLSDEVRNKPMDIKNMLIDIGADDKEDAKKIGIRPGQQIV
                     PICPFTPMANEKKILAKAWDNRYGCGLAIELLKEVHGETLPNTLYSGANVMEEVGLRG
                     AQAAARMINPDLFFALDASPANDASGNKKEFGQLGKGTLLRIFDRSMVTHRGMREFIL
                     DTAESNNIPYQYFISMGGTDAGQVHISNEGVPSAVIGICSRYIHTSASIIHTDDYAAA
                     KELLVKLVKACDKTTVETIKSNV"
     gene            1861..2382
                     /locus_tag="VFX34_07470"
     CDS             1861..2382
                     /locus_tag="VFX34_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016202837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HEX5564782.1"
                     /translation="MKFIIGSTNQAKVKAVQAVIESYFPGSGLSEMKVPSGVKDQPFG
                     EEETRTGALNRATNAAKELEGAIGIGLEGGVRMLGDEMFVCNWGALVLPSGKSFTAGG
                     AQIPLPDEIAIELQKGRELGVVVDEYFKAGGIRHSEGAIGMFTAHVVTRDKLFEHVMQ
                     LLIGQLKYYEPAN"
     gene            2466..2981
                     /locus_tag="VFX34_07475"
     CDS             2466..2981
                     /locus_tag="VFX34_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564783.1"
                     /translation="MFRLWKFLFLALLSISVLTACAKTFDERAAEGIAAAKEAFLLHD
                     QHVNDEVHGVPLYKPAGFTIDEKSDAQNIVMKKGDDTFLLFINPNEARDSRLFYDLLT
                     QKEDKERYEEVFTDDGNFGFASIVKKGEDQVELIASVGGIKMTTITKNKNIEEDMGKM
                     MEVVRSIEQGS"
     gene            3053..>3522
                     /locus_tag="VFX34_07480"
     CDS             3053..>3522
                     /locus_tag="VFX34_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 family protein"
                     /protein_id="HEX5564784.1"
                     /translation="MNSQQLVDLLKQHLPSEHLQWRFDRKTDKIRLEHSVLKKGMDIS
                     LPEILAKYETKQEAAINEVVYTIRETFKAMEKEQAEGFVKENAIYPVIRSTSFPQTSK
                     AGDRFIVKDHTAETRIFYALDLGTTYRLIDESMLDSIGMTESEIMESALFKVRS"
BASE COUNT         1039 a          752 c          854 g          877 t
ORIGIN      
        1 tttgcgaagg aaaagcttat gaatgaagca ttgcctagcc gatatcgttt catcttctac
       61 catgatgcgg tttaccgtct cgtccaatgg gatgaaggcg gcaaagaaat taccgaatcg
      121 gtcgagcgaa caagatcatt aacataaaaa agcaacaggt gaaagggaat tgttatgctc
      181 cttcgcctgt tgcttttaat atacaaattt tcttttcatt tttgaatcaa ttcgacgaca
      241 gtgcctgttt gcgcgttagc cgcaaattca aattgctcca gttcgccatt ccggattctt
      301 gtaatgcctc cacggtacac ttccatggat agaacattgt ttgtaaacgg ctcaggtttc
      361 atgacaatcc atgaaccatc aatcggacct tcttttttga aggcatcctt cactgcatcc
      421 aagacatgtt ccgccggagt gttcgggttc atctttccga ttgcctgatt gacaacaagc
      481 ccggcagcaa cgcctgttaa aactcctgct acgaaatctt tcactttcat gcctgagaaa
      541 ccccctccat caatcttccc caagtgtacc atatttcaaa aaaatgttta actgttgaaa
      601 tgaacattcg ttttgtgtag ggaaattagt tcggaaagct aaatattatg atggaaagtt
      661 tcaaatacgt gcactatact agaagaagta catatcaaat agggggaaac aattcatgaa
      721 caatgaaacg ttgactatgt tcaagacatt gaccgagttg cctggagcgc ctggaaatga
      781 gcatttggtc cgcaattata tgcgggaaga actcggcaag tattccgatg aaatcatcca
      841 ggacaacctc ggtggagtat tcggtgtgcg gaaaggtcct gaaaacggtc cgcgcatcat
      901 ggtcgccggt catatggatg aagtaggatt catggtcacc gcaattaccg ataacggcat
      961 gctccgtttc cagccgctcg ggggatggtg gagccaagtg ctattggcgc aacgtgtcca
     1021 gatcatcacg gatgaaggtc ctgtcgtcgg tgttatcggc tccattccgc cacatcttct
     1081 cagtgatgaa gtccgtaaca aaccgatgga tattaaaaac atgcttatcg atatcggcgc
     1141 ggatgataaa gaggacgcaa aaaaaatcgg catccgtccc ggccagcaaa ttgtgcctat
     1201 ctgcccgttc acgccaatgg caaatgaaaa gaagatcttg gcaaaagcat gggataaccg
     1261 atacggctgc ggtttagcaa ttgagctgct gaaagaggtt cacggcgaaa cccttccgaa
     1321 tacgctttac tccggtgcaa acgtcatgga ggaagtcggt ttgcgcggag cacaggcagc
     1381 tgcaagaatg atcaatcctg atttgttctt cgcattggat gcgagtcccg cgaatgacgc
     1441 ttccggtaat aaaaaagaat ttggccagct tggaaaaggg acattgctgc gcattttcga
     1501 ccgttcgatg gtgacgcacc gtggaatgcg tgaattcatc ctcgatacag cagagtccaa
     1561 taacattcca tatcaatatt tcatctccat gggagggacg gacgccggac aagtccatat
     1621 ttcaaacgag ggcgttccaa gtgcagtcat cggtatctgc tcacgctaca tccacacatc
     1681 cgcttccatc atccacaccg atgattacgc ggcggcgaaa gagcttctcg ttaaattagt
     1741 gaaggcttgt gataaaacaa cggttgaaac gatcaaaagc aatgtgtaac tgaagagtga
     1801 atatggccat cctttcagaa acattgagga tggctttttt ctgacagaaa ggagaacagc
     1861 atgaaattca tcataggttc caccaatcaa gcgaaggtga aagcggtaca ggcagttatt
     1921 gaaagctact ttccgggaag cgggttatct gaaatgaaag ttccatccgg cgtgaaggat
     1981 caaccttttg gagaggaaga aacaagaacc ggagcattga atcgcgctac gaatgccgca
     2041 aaagaattag aaggagctat cggcatcggc ttggagggcg gtgtacggat gcttggagat
     2101 gaaatgttcg tttgcaactg gggggcgctt gtgctgcctt ccgggaagtc atttacagcc
     2161 ggaggggccc aaattccctt gcctgatgaa attgctatcg aattgcagaa aggccgtgaa
     2221 ttgggagtcg tcgtcgatga atatttcaag gcgggaggca tccgccatag cgaaggggca
     2281 atcgggatgt tcaccgccca cgtggttacc cgtgacaagc tgttcgaaca tgtcatgcaa
     2341 ttattgattg gccaattgaa gtactacgaa ccggctaact aatacgcttg atttgcatat
     2401 acgtaacatt atctttaaaa taggtaacgg tatttgaata aaaatgtacg attggaggaa
     2461 gctgaatgtt ccgcctttgg aaattcttat tcctggcact cctatccata tctgtattaa
     2521 ccgcatgcgc caaaacgttc gatgaacggg ccgcagaggg gattgcagct gcaaaagagg
     2581 catttctatt gcatgatcaa catgtgaatg atgaagtaca tggtgttccg ttatataaac
     2641 cggccggatt tacgattgac gagaagtcgg atgcccaaaa tattgtcatg aaaaagggcg
     2701 atgatacttt tctgctattc attaatccga atgaggctag ggatagtagg ctcttttacg
     2761 atctgttgac ccaaaaagag gacaaagagc gatacgaaga agtattcacc gacgatggga
     2821 atttcggttt tgcctcaatt gtcaagaagg gtgaagatca ggtcgaactc attgcaagtg
     2881 tcggcggaat caagatgact acaataacta agaacaaaaa tattgaagag gatatgggca
     2941 agatgatgga agtggtacgt tccatcgagc aaggctcata atcaattaca aatcggtcac
     3001 ccggcgcaat tgcggcgagc ggccgtttat ttttgaagaa ggggtgatgt gcatgaattc
     3061 acaacaatta gtggaccttt taaaacaaca tttgccatcc gaacatttgc aatggcgctt
     3121 cgatcggaaa accgataaaa tccgtttaga acattctgta ctgaaaaaag gaatggatat
     3181 ttccttgccg gaaatccttg caaaatacga aacaaaacaa gaagctgcca tcaatgaagt
     3241 cgtctacaca atacgcgaga cattcaaagc gatggaaaaa gagcaggcgg aaggattcgt
     3301 gaaagaaaat gcaatctatc cggtgatccg ctccacttcc ttcccgcaaa cttccaaggc
     3361 gggagatcgt ttcatcgtga aggatcatac agcggaaaca agaattttct atgcgcttga
     3421 tttgggcacg acataccggc tcatcgatga atcgatgcta gattccatag gcatgacaga
     3481 atccgaaata atggagtcgg cgctgttcaa agtaaggtcc tt
//
LOCUS       DASULI010000351        13300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1570132,
            whole genome shotgun sequence.
ACCESSION   DASULI010000351 DASULI010000000
VERSION     DASULI010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 13300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13300
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570132"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VFX34_07485"
     CDS             <1..135
                     /locus_tag="VFX34_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5564785.1"
                     /translation="QPEVVGSIESEFISGVAKVDKRLLVMLNLEKVLQPVKRVATDEI
                     "
     gene            125..757
                     /locus_tag="VFX34_07490"
     CDS             125..757
                     /locus_tag="VFX34_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEX5564786.1"
                     /translation="MKSDHQITDMHLDVLKEIGNIGAAHAATSLSQLLGRKIDMLVPK
                     VSLVSFDEMFDLAGGAERIVAGIYLRIQGDLSGSMFFVLPIDSANEFIRRLIGDETFN
                     FHSVPIPEMGISAIQELGNILSGSYLSALSDFTGLKVHPTVPSLSVDMVGAIVSFGLI
                     EVSHYSDEVIVIDTRIEEEGEDGNSSVDGHFFLLPDPESYITIFRSLGVL"
     gene            765..1253
                     /locus_tag="VFX34_07495"
     CDS             765..1253
                     /locus_tag="VFX34_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX5564787.1"
                     /translation="MVKTVVRVGIADMNIVQAPDSIRTSGLGSCVGVVLYDETKKIAG
                     LLHVMLPDSSLGRSDTLNAAKFADTGIYSLMEMLKAEGVRPLSLKAKIAGGSQMFQFG
                     SSDSIRIGPRNVEAVKKELARLSIPIIAEDTGGSSGRTIEFDPSTSSLSIRTVNLGTK
                     EI"
     gene            1386..2168
                     /locus_tag="VFX34_07500"
     CDS             1386..2168
                     /locus_tag="VFX34_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX5564788.1"
                     /translation="MSKEDLTEEDACWKLWLNDRDPDAGDRLVRKYTPLVNYHVQRIG
                     SGLPRNVSRDEIMSLGLQGLFDALTKFDPGRDLKFDTYASFRIRGSIIDGLRKEDWLP
                     RSSREKSKKLEEEISKLEQKLLRHATPEEIADHLGIPVEDVYQTVHEHYFSNVLSMDE
                     RMSDDDEEGQKSFIIKDDHERTPEQQIVMGELVGDLAVKIKELNQNEQLVLNLFYTDE
                     MTLTEIGEILNLSTSRISQIHSKALFKLRKLLLPEIVDGGIL"
     gene            2165..2467
                     /locus_tag="VFX34_07505"
     CDS             2165..2467
                     /locus_tag="VFX34_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEX5564789.1"
                     /translation="MSLKGIELQIAIPKTFDAGKVAEQKQQQSQLNQDQAQLRNEKEA
                     LKNRESVRQSEKYAKMDKDSKGKGQDKERKEIERQHNEEEEKTRHPYKGSFVDFTG"
     gene            2473..2988
                     /locus_tag="VFX34_07510"
     CDS             2473..2988
                     /locus_tag="VFX34_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5564790.1"
                     /translation="MQMVFLLFLFIIQVIGFYFLALLYMKVSKFNNLEKKQEKLMEEM
                     DDAIGAYLSELKEENERLISIIEKRQKNDTLRDHVPETHQNTEPEQQAGTKAFSIKPP
                     SYPVKAAMKSYQTAPVLKKGGLIEPETDEPELDDRTLAIRMHDDGKPIEEIAKRLGKG
                     KTEVELLLKFR"
     gene            3133..3864
                     /gene="rpsB"
                     /locus_tag="VFX34_07515"
     CDS             3133..3864
                     /gene="rpsB"
                     /locus_tag="VFX34_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5564791.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMKKYIFVERNGIYIIDLQ
                     KTVKKLEEAYDFMRQVGADGGKVLFVGTKKQAQDAIKEEAERAGMYYINQRWLGGTLT
                     NFGTIQKRVARMKKIETMEEDGTFDVLPKKEVSELRKEHDRLVKFLGGIRDMKSLPDV
                     IFVVDPRKERIAVAEAIKLNIPLVGIVDTNCDPDEIDYVIPANDDAIRAVRLLTSKMA
                     DALIESRQGADEEVSEEATETAAAE"
     gene            4043..4927
                     /gene="tsf"
                     /locus_tag="VFX34_07520"
     CDS             4043..4927
                     /gene="tsf"
                     /locus_tag="VFX34_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5564792.1"
                     /translation="MAITAQMVKELREKTGAGMMDCKKALTEVNGDMDAAVDFLREKG
                     LSSAAKKADRIAAEGTTDIIVNGNDAVIFEVNAETDFVAKNEAFQALVKELGEHLLAT
                     KPATVEEAAESKMENGLSVADHISNAVAKIGEKITLRRFEVKTKTDNDAFGPYLHMGG
                     RIAVLTILEGTTDSEAAKDVAMHIAAMNPKYISRDEVSEEEVERERKVLTEQALNEGK
                     PENIVAKMVEGRLGKYFEEICVLDQAFVKNSDQKVRDFVKSTGGTLKEFVRFAVGEGI
                     EKREDNFADEVMSQVKGN"
     gene            5006..5731
                     /gene="pyrH"
                     /locus_tag="VFX34_07525"
     CDS             5006..5731
                     /gene="pyrH"
                     /locus_tag="VFX34_07525"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5564793.1"
                     /translation="MGVPKYKRIVLKLSGEALAGEKGYGLSPAIIKTVAEQVKEVVDL
                     GVEVAVVVGGGNIWRGKVGSEMGMDRTTADYMGMLATVMNSLALQDALEKLDVETRVS
                     SSIDMRQVAEPYIRRKAIRHLEKKRVVIFAAGTGNPYFSTDTTAALRAAEIEADVILM
                     AKNNVDGVYSADPLKDENAVKYTELTYLEVISQGLEVMDSTASTLCMDNDIPLVVFSI
                     MDNGNIKKAVLGEPIGTVVRRNG"
     gene            5732..6289
                     /gene="frr"
                     /locus_tag="VFX34_07530"
     CDS             5732..6289
                     /gene="frr"
                     /locus_tag="VFX34_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5564794.1"
                     /translation="MPKAVMDQTKDRMEKAIGSFSRQLASIRAGRANASLLDRISVLY
                     YGAPTPLNQMAGISVPEARLMVIQPYDKTTLGDIEKAILKSDIGITPSNDGSVIRLAV
                     PALTEERRKELVKEVKKEAEDSKIAIRNVRRDANDELKKLEKNGEITEDELRREGDEI
                     QKLTDSYIVKIDEIAKEKENEIMEI"
     gene            6511..7305
                     /locus_tag="VFX34_07535"
     CDS             6511..7305
                     /locus_tag="VFX34_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX5564795.1"
                     /translation="MLDKLLRKKTVENQPSISDRLTKVKSRTIPSHVAIIMDGNGRWA
                     RRRNLPRIAGHHEGMKTVRKITRIANDIGIKVLTLYAFSTENWKRPKMEIEFLMKLPG
                     EFLTTYLPELIEQNVRVEMIGNFDALPPHTKEAIVKAIEATSNNDGLILNFAMNYGSR
                     LEMVEAVKEIAKLAVDNRIAIDEIDESLIESRLMTSHLPEPDLLIRTSGEVRLSNFML
                     WQLAYSEFSFTEVLWPDFDESCMLSAIEEFQMRNRRFGSVEGDKGV"
     gene            7302..8096
                     /locus_tag="VFX34_07540"
     CDS             7302..8096
                     /locus_tag="VFX34_07540"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5564796.1"
                     /translation="MKQRIITAIAALAIFFPLVIIGGLPFTIAIYIIASVGLYELLRM
                     KDIRIFSFEGLLVWIALAILLMPSRWTVEFQQWIGYTKIEMIFALILILLIYTVISKN
                     KFTFDHAAFTAFSALYVGIGFYYLIETRFYGFEYVFYALLVIWTTDSGAYFVGRKIGN
                     RKLWPDISPNKTVEGFVGGIVFAVLFACVFQIFYPIAPSYLMLVIVTIIASIVGQLGD
                     LVESALKRHFGVKDSGNLLPGHGGILDRFDSLLFVLPLIHFLHLVG"
     gene            8119..9267
                     /locus_tag="VFX34_07545"
     CDS             8119..9267
                     /locus_tag="VFX34_07545"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5564797.1"
                     /translation="MTKKISLLGATGSIGTQTLDIIQSNKEQFKLVSFSAGMNIPKVK
                     EITAQFHPDIVSVMQKEDADSLQAEFPETRFVHGKEGLIEVAAHAGADMLVNAVIGSI
                     GLEPTMEAIREGITIAIANKETLVAAGDIVMEEARKRDVALLPVDSEHSALFQSLNGE
                     NPKRISRLILTASGGSFRDLTRDQLQGVTVQQALDHPNWSMGNKLTIDSATMMNKGLE
                     VIEAHHLFGMPYDKIDCLLHKESIIHSMVEFEDTSVMAQLGSPDMRVPIQYAMTYPDR
                     IPMQNAKPLRLEEIGMLHFEKMDFERFKALALAYEAGRQGGTMPTVMNAANEVAVQLF
                     MKGIISFLKIEEIVERMMDNHQSISSPDLETILETDSLTRNMVYDMVK"
     gene            9312..10571
                     /gene="rseP"
                     /locus_tag="VFX34_07550"
     CDS             9312..10571
                     /gene="rseP"
                     /locus_tag="VFX34_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5564798.1"
                     /translation="METVIAFIIIFGSLVFFHELGHFLFAKKAGIMVREFAIGFGPKI
                     LAIRKKETLYTIRLLPLGGYVRMAGEDFDTVELQPGYRVGVILNTDDEIEKIYLNRDV
                     SNPNVLFLETESADLSKGLFIEGYDEDGKLVNYKVSRNAVIYEKGQETLIAPYDRQFD
                     SKSLGSRAMAIFAGPLFNFILAFFIFLAIGLLHGIPTNEPIIKEVISDSPAYAAGMEA
                     GDFVKEVDGKPVETWQEFTETIQANPENRLQMKVERDGEMVTLAVVPEKAVKEGREFG
                     QIGVMYESPVEKNVLKAFAFGAEQTFSWMKRIFELLGMLVTGKFTIDALSGPVGIYKA
                     TEEVAQWGVFSLMNWAAVLSINLGIMNLLPLPALDGGRLLFFAFEGVRGKPVDKQKEG
                     MVHFVGIMLLMVLMLVVTWNDIQRFFF"
     gene            10612..12312
                     /locus_tag="VFX34_07555"
     CDS             10612..12312
                     /locus_tag="VFX34_07555"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5564799.1"
                     /translation="MKQSRTFIPTMRETPTDADILSHQLLLRAGYIRQHMSGVYSFLP
                     LAKKVIRKIENIIREEMEKIDAVEMFMPTLQSSGLWKETGRLDSYGAELFRIKDRHER
                     QMVLGPTHEEVVTSLIRDEVKSYKKLPLTVYQIQTKFRDEKRPRFGLLRGREFIMKDA
                     YSFHSTEESLEDKYNEMMQAYSNIFSRLDLDFRAIIADGGTIGGEGSHEFMAVSDIGE
                     DTIAYSDASSYAANIEMAEVKAEYEKSKETLKELTKVETPGQRTIAEVSDYLNVDPSR
                     VIKTLIFKADDKLVVVLTRGDHDINEIKLKNALQADEIELATEDEIEEMLSCKVGSIG
                     PVKLPLDLKVYADYGVEYIVNGVCGANEDGWHLVNVNPERDFAIEQYFDLRFIKEGDP
                     SPDGNGSIQFTKGIELGHVFKLGTTYSGPMKATYLDENGKAQPYIMGCYGIGVSRILA
                     AVAEQHNDANGLKWPKQLSPYDIHLVTINMKDEAQTNLSEELYHLLKSYRYEILHDDR
                     DERPGVKFTDADLIGLPIRLTIGKKADEGIVEVRFRSTGETVEWQKEEVTEKLQAFFG
                     "
     gene            12388..>13300
                     /locus_tag="VFX34_07560"
     CDS             12388..>13300
                     /locus_tag="VFX34_07560"
                     /inference="COORDINATES: protein motif:HMM:NF022927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III N-terminal
                     domain-containing protein"
                     /protein_id="HEX5564800.1"
                     /translation="MDAKVKLLTLLQQIGMTDDQTIAHFEHGTLERVDIHRKSRVWQF
                     NVELEKTLPVQVYQLFQQRMSEAFTSIASVRLQLKVRNTEGDPELIVGYWPHVIEEMR
                     DMSPPIRERLIGQQPVLVGGKMTLTCANDFEQQTMKNKYGSLIAEVYESFGFPSMVVE
                     FAISEEADDNEAEILAFEAQKKAEEEEYAKNALANLQKMEAMKKENGDAGNRPFVLGV
                     PIKPDEPIIDIQTIQDEERRVTIEGYVFDAEVRELRSGRSLLTIKVTDYTDSILVKMF
                     SRDNEDAELMKTLKKGAWVRARGSIQND"
BASE COUNT         3982 a         2681 c         3365 g         3272 t
ORIGIN      
        1 cagccggaag tcgtcggatc cattgaatct gaattcattt cgggagttgc caaggtcgat
       61 aaacggctat tggtcatgtt gaatcttgaa aaagtgctcc aacccgtgaa aagggtggca
      121 acagatgaaa tctgatcatc agattaccga tatgcatttg gacgttctga aggaaatcgg
      181 caatataggg gcggcccatg cggcaacttc tttgtcccaa ttgctcggca ggaagatcga
      241 tatgctcgta ccgaaagttt cgctcgtatc cttcgatgaa atgtttgatt tggcaggggg
      301 agcagagcgg attgtggcag gtatatacct acgaatccaa ggtgatctgt ccggaagcat
      361 gttctttgtc ttgccgatcg attccgcaaa tgaattcatc cgaaggttaa ttggagatga
      421 aacgttcaac ttccattcag taccgattcc ggagatgggc atctcggcaa ttcaggaact
      481 cggcaatatt ttgtctgggt cctatttgtc cgcattgtct gatttcaccg gattgaaagt
      541 ccatccgacc gttccatcgc taagtgttga tatggtaggg gcgattgtca gcttcggcct
      601 catagaagtc tcacattata gcgatgaagt cattgtcatt gatacgcgta tcgaggagga
      661 aggcgaagac ggaaattcaa gtgtcgacgg ccatttcttc ctgcttcctg atcccgagtc
      721 gtatattacg attttccggt cgttaggtgt actgtgagat gagcatggtg aagacagttg
      781 tgagggtggg catcgctgac atgaatatcg tccaagcgcc ggattccatc cggacatcag
      841 gtttagggtc atgcgtcgga gtcgtgctat atgacgagac gaagaagatt gcgggacttc
      901 ttcatgttat gcttcccgac tctagtctcg gaaggtctga cacgttgaat gctgctaaat
      961 ttgcagacac cggcatctat tcgcttatgg aaatgctgaa ggcggaaggg gttcgcccct
     1021 tatcattaaa agccaaaatc gctggtggtt cccaaatgtt tcaatttgga tccagcgatt
     1081 ccatccgtat cggccctcgg aatgtcgaag ctgttaaaaa ggagcttgcc cgactatcca
     1141 ttccgatcat agcggaagat acgggcggtt ccagtgggcg gacaatcgag tttgacccgt
     1201 cgactagctc tttgagcatc cggacggtta atttgggaac gaaggaaatt tgaggcggaa
     1261 tccaatggaa tagacgtaac agagcgactg aacggttcag tcgctatttt ttgtcggaat
     1321 ttgctataat agaaatcaaa caatttgttt ttttgtctga aacatcatgg aaggtgaggg
     1381 attcgatgtc caaagaggat ctgacggaag aagatgcatg ttggaaacta tggttgaatg
     1441 atcgggatcc cgatgccggc gatagacttg tccggaagta tactcctctc gtcaattacc
     1501 atgtacagcg tattggctcc ggcctgccgc ggaatgtttc gagagatgaa atcatgagcc
     1561 tcggtcttca agggcttttt gatgctttga cgaaattcga tccgggaagg gacttgaaat
     1621 ttgatacata tgcttccttc aggattcgtg gatcgatcat cgacggattg cgaaaggaag
     1681 actggctgcc gcgttcatcc cgtgaaaaat ctaaaaaact agaagaagag atttcaaaac
     1741 tggagcagaa actgctgcgt catgccaccc ccgaagaaat agcggaccat cttggaattc
     1801 cagtcgaaga tgtctatcag acggtgcatg aacactactt ctcaaatgtg ctctccatgg
     1861 atgaacgtat gagtgatgat gatgaggaag gacagaagtc ttttatcatc aaggacgatc
     1921 atgagcgtac gcctgaacag caaatcgtca tgggagaact tgttggggat ctggccgtga
     1981 aaatcaaaga actgaaccaa aatgaacaat tggtgttgaa cctgttctac acagatgaaa
     2041 tgacattgac ggaaataggg gaaatcttga acttatcaac atccagaatt tcgcagatcc
     2101 attcgaaggc attattcaaa ctgcgaaagt tactgttgcc ggaaattgtc gacggaggga
     2161 ttttatgagc ctgaaaggaa tcgaactcca aatcgccatc cctaaaacgt tcgatgcggg
     2221 aaaggtggca gaacaaaaac aacaacaatc ccagctcaat caggatcaag cccagcttcg
     2281 gaatgaaaaa gaggcattga aaaaccggga aagtgtccgg caatccgaga aatatgccaa
     2341 aatggacaaa gactcaaaag ggaaaggtca ggacaaagaa aggaaagaaa tagaacgtca
     2401 acataacgaa gaggaagaga agacgaggca cccttacaaa gggtcgtttg tcgactttac
     2461 aggatgatca gcatgcaaat ggtattttta ttatttttat tcattattca agtgatcggg
     2521 ttttatttct tagcgttgct atacatgaaa gtttccaaat tcaacaatct ggagaagaag
     2581 caggagaaac tgatggagga aatggatgat gcgatcggtg catacttatc cgaattgaaa
     2641 gaggaaaacg aacggctcat cagcatcatc gaaaaacggc agaagaatga cacgcttagg
     2701 gatcatgtgc ccgaaacaca tcaaaataca gaacctgaac agcaagcagg gacgaaggcg
     2761 ttttccatca aaccgccaag ttatccagtg aaagccgcta tgaaatccta ccagacggct
     2821 cccgttttga aaaagggggg cttgatcgaa cctgaaacgg atgaacccga attggatgat
     2881 cgcacactcg ccatccggat gcatgatgac ggaaagccga tcgaagaaat tgcaaaaagg
     2941 cttggcaaag ggaaaacgga agtggaattg ctgctgaaat tcagatgaag tgagttgcaa
     3001 tcggattatc agtatggtat attatcaaat ggtgttacta cacacgtatg tggaggatgg
     3061 gaataggtgc cgaaaggtca tcccgtcgca tgaaacatgc ggaggaatct aaccaaattg
     3121 gaggaaaaac aaatgtcagt aatctcaatg aaacaactgc ttgaagcagg tgtacatttc
     3181 ggacaccaaa ctcgtcgttg gaacccgaaa atgaaaaagt atattttcgt tgaacgtaac
     3241 ggaatctaca tcatcgatct tcaaaagacg gtgaaaaaac tagaagaagc ttatgacttc
     3301 atgcgtcaag tcggtgctga cggcggaaaa gtccttttcg tcggaacgaa gaagcaagca
     3361 caagatgcta tcaaagaaga agctgagcgc gcaggaatgt actacatcaa ccaacgttgg
     3421 cttggtggta cacttacaaa cttcggtacg atccaaaaac gtgttgcacg tatgaaaaag
     3481 atcgaaacga tggaagagga cggtacattt gacgtacttc ctaaaaaaga agtttctgaa
     3541 ctaagaaaag aacacgatcg tcttgttaaa ttccttggcg gtatccgtga tatgaaatca
     3601 ttgcctgacg tcatcttcgt tgttgaccca cgtaaagagc gcatcgctgt ggctgaggca
     3661 atcaaattga acattccact cgtcggtatt gttgatacga actgtgatcc ggacgaaatc
     3721 gactatgtca tccctgcgaa tgatgatgca atccgtgcgg ttcgtctact tactagcaaa
     3781 atggcagatg cattgatcga atcaagacaa ggcgctgatg aagaagtatc tgaagaagcg
     3841 actgaaacgg cagctgcaga gtaatagtag taatagaaca gacgataagc ggccaacccc
     3901 cttatcgtct tttttagaga atgggacttg attgttgata gtagtaagct ctgttgattg
     3961 aagcggaaag cgtccgcctg cagctgaaat caacaatgtc gaagtttgtc gtctcaaatc
     4021 actcaaacag gaggaactaa caatggcaat tacagcacaa atggtaaaag agctgcgcga
     4081 aaaaactggc gcaggcatga tggactgcaa aaaagcactt actgaagtaa acggagatat
     4141 ggatgcggcg gtagacttcc ttcgcgaaaa aggtctatca agcgcagcta aaaaagctga
     4201 ccgcatcgct gcggaaggta caactgacat cattgtcaac ggcaatgacg cagtcatttt
     4261 cgaagtgaat gctgagacgg acttcgttgc gaaaaacgaa gcgttccaag cacttgttaa
     4321 agaacttggt gagcaccttc ttgcaacaaa accggctacg gttgaagaag ctgcagaatc
     4381 gaaaatggaa aacggccttt cagtcgcaga ccacatttct aatgcagtcg ctaaaatcgg
     4441 tgagaaaatt actcttcgcc gctttgaagt gaaaacaaaa acggacaacg atgcattcgg
     4501 tccttacctt cacatgggtg gacgcattgc agtattgaca atccttgaag gcactactga
     4561 ctccgaagct gctaaagatg ttgccatgca catcgcagcg atgaacccga aatacatttc
     4621 acgtgatgaa gtgtccgagg aagaagtgga gcgcgagcgc aaagtcctta ctgagcaagc
     4681 gttgaacgaa ggcaagccgg aaaacatcgt tgcgaaaatg gttgaaggcc gtcttggaaa
     4741 atacttcgaa gagatttgtg ttcttgacca ggcatttgtt aaaaactccg atcaaaaagt
     4801 ccgcgatttc gttaaatcga ctggcggaac attgaaagaa tttgttcgct ttgcagttgg
     4861 tgaaggaatc gaaaaacgcg aagacaactt cgctgatgaa gtcatgagcc aagtaaaagg
     4921 aaactgagca gatataaaga tcaattaggg gacacaattt tgtgttccct atttttcgag
     4981 aaaacacagt agacggaggg tacatatggg cgtcccaaaa tataaaagaa ttgtcttgaa
     5041 gcttagcggt gaggcgcttg ctggtgaaaa aggctatggt ctatcaccgg caatcatcaa
     5101 aacagtcgca gaacaagtga aagaagttgt cgaccttggc gtggaagttg cggtcgtagt
     5161 tggtggagga aacatttggc gaggtaaagt cggaagcgag atgggcatgg accggacaac
     5221 cgccgattat atgggcatgc tcgccacagt catgaattca ttggctcttc aggacgctct
     5281 tgaaaaatta gacgttgaaa cacgtgtatc atcatcgatt gatatgcggc aagtggctga
     5341 gccgtatatc cgccggaaag cgatccggca tctggagaaa aagcgtgtcg taatctttgc
     5401 agctggtaca gggaatcctt atttctcaac agatacgaca gctgcactta gagcggcgga
     5461 aatcgaagca gacgtcatct taatggcgaa aaataatgtt gatggtgtct attcagcaga
     5521 tccattaaaa gatgagaatg cagtgaaata tacggaattg acgtacttgg aagtaatcag
     5581 ccaaggactc gaagtcatgg attccacagc ttcaacccta tgtatggaca atgatatccc
     5641 tctcgtagta ttctcaatta tggataatgg aaatattaaa aaggccgtac ttggggaacc
     5701 aatcggtacg gttgtgagga ggaatgggta aatgccgaaa gcggtaatgg accaaacgaa
     5761 ggacagaatg gaaaaagcga tcgggtcttt ctcaagacag cttgcttcca tccgagcagg
     5821 acgtgcgaac gcttcattgc tggatcggat ttcagtgctt tattacggag caccgacgcc
     5881 gttgaaccaa atggcaggca tctcagtgcc agaggcgaga ctgatggtca tccagccata
     5941 cgataaaacg actttgggtg atattgaaaa ggcgattttg aaatcggata tcggtatcac
     6001 tccttccaac gatggatcgg tcatccgttt ggcagtccct gctttgacgg aagaacgacg
     6061 caaggaattg gtgaaggaag tcaaaaagga agcggaagac tccaaaattg caatccgcaa
     6121 cgtacgccgg gatgcaaatg acgagttgaa gaagcttgaa aagaatgggg aaatcacaga
     6181 ggatgaacta cgccgtgaag gtgatgaaat tcaaaaactg actgattcct acatcgtgaa
     6241 aatcgatgag attgccaaag aaaaagaaaa cgaaatcatg gaaatctgat cgaagaactt
     6301 tctatacatt ggggcgtcct actttcagga cgcctttttt tctgaccgta tttcattcgt
     6361 tttcccgaat gataaatatt tcaccatatg gaatgcataa tctaaaagag acgtaacgca
     6421 aatttttact gttttaaatg aaatttatcg gccgatttgt tacgatagac taggaacttg
     6481 ctcaaaaagc aggcgagtgg aggaaacgga atgctagata aactgttacg gaaaaaaact
     6541 gtagagaacc aaccatcaat ttccgacagg cttacaaaag tcaaaagtag aacaatacct
     6601 tcacacgtcg ccatcattat ggatgggaac ggcagatggg caagacgacg caacttaccg
     6661 cggattgccg gacatcatga agggatgaaa accgtccgga aaatcacccg gatcgcaaac
     6721 gatattggca tcaaagtcct gacattgtac gcattttcaa cggagaactg gaaaaggccc
     6781 aaaatggaaa ttgaattcct tatgaaactg ccaggagagt tccttacaac gtacttgccc
     6841 gaattgatcg agcaaaacgt cagagttgaa atgatcggca attttgatgc gttgccgcca
     6901 catacaaaag aggcgattgt caaggcaata gaggccacct caaacaatga cggcctcatc
     6961 ctgaacttcg ccatgaacta tggaagccgc cttgaaatgg tggaggcagt gaaggaaatc
     7021 gccaaattgg cagtagataa ccgaatagca atcgatgaga ttgacgaatc attgattgag
     7081 tcacgtctca tgacatctca tttacccgaa cccgatctgc tcatccggac gagtggggaa
     7141 gtgagattgt cgaatttcat gctttggcag ctggcttatt cggaattttc ctttacagaa
     7201 gtcctctggc cggactttga tgagtcatgc atgttgagtg caatcgagga attccaaatg
     7261 cgcaaccgaa gatttggaag tgtggaaggg gataaaggag tttgaaacag agaattatca
     7321 cagcgatcgc tgcattggcc atatttttcc cgcttgtcat tatcggcggg ctcccattta
     7381 caatcgccat ttatatcatc gcttcagttg ggttatatga attgctgcga atgaaggaca
     7441 ttcggatatt ttctttcgag ggccttcttg tctggattgc actggccata ttattaatgc
     7501 cgtcaaggtg gactgttgaa ttccaacagt ggatcggata tacaaaaatt gaaatgatat
     7561 tcgcacttat tcttatttta ttgatttata cagtcatctc taagaacaaa ttcacttttg
     7621 accatgccgc attcacagca ttcagtgcat tatacgtcgg catcgggttt tattatttga
     7681 ttgaaacccg cttttatgga tttgaatacg ttttttatgc acttttagta atttggacta
     7741 ctgactcggg tgcatatttc gttggcagaa agattggcaa tcgaaaactt tggccggata
     7801 tatccccgaa taagacagtg gaagggtttg ttggcggtat cgtctttgcc gtcttgtttg
     7861 catgtgtgtt ccaaatcttt tatccgatag cgccttctta tttgatgttg gtcattgtaa
     7921 cgatcatcgc ctcgattgtc ggacagttgg gcgatttggt cgagtcagcg ttgaagaggc
     7981 atttcggagt gaaggattcc gggaatcttt tgccgggaca tggaggaatt cttgatcgat
     8041 tcgatagtct ccttttcgtg ttgccgctca ttcatttttt acacctagtc ggttaatgga
     8101 aaggaagata ttcatattat gacgaaaaaa attagtctgc ttggtgcaac gggatcaatc
     8161 ggcacgcaga ctctcgatat tattcaatct aacaaagagc aattcaaact cgtatctttt
     8221 tcagccggga tgaatatacc gaaagtgaag gaaatcacag cacaattcca tccggacatc
     8281 gtatctgtca tgcagaagga agatgcagat tccttgcaag ctgaatttcc ggaaacacgc
     8341 tttgtgcacg gcaaggaagg cctcattgaa gtggcggcac atgctggcgc ggacatgctc
     8401 gtcaatgcgg ttatcggcag catcggactt gaaccgacaa tggaagcgat tcgtgaaggc
     8461 atcacaatcg ccattgcgaa caaggagacg ctcgtagctg ctggagatat cgtcatggaa
     8521 gaagcaagga agagggatgt agcgcttctg cctgtcgaca gtgaacattc ggctcttttc
     8581 cagtccttga atggcgaaaa tccgaaacgg atttccaggc tgattctgac ggcttccggc
     8641 ggtagtttca gggatttgac aagggaccaa ctacaaggcg tgaccgtaca gcaggcgctc
     8701 gaccatccga actggtccat gggcaataaa ctgacgattg actctgctac aatgatgaac
     8761 aaaggcctcg aagtgatcga ggcgcatcat ttattcggta tgccgtatga caagatcgat
     8821 tgcttattgc acaaagaaag catcatccat tcaatggtgg agtttgaaga cacaagtgtc
     8881 atggcgcagc ttggatcccc ggatatgcgg gtgccaatcc agtatgcaat gacctacccg
     8941 gaccggattc ctatgcagaa tgccaagccg ctccgtcttg aggaaattgg aatgctccat
     9001 ttcgaaaaga tggatttcga aagattcaag gcgctggccc tcgcgtatga ggcaggcagg
     9061 caaggcggga cgatgccgac agtgatgaat gctgcaaatg aagtcgctgt ccaactgttc
     9121 atgaaaggca taatatcttt cctcaaaatc gaagagatcg ttgaacggat gatggacaat
     9181 catcagtcga tttcttcacc ggatttggaa acgattttag aaaccgattc actcacccgt
     9241 aatatggtgt atgatatggt gaaatgagta attcataaat cggccgaatg ggccagttaa
     9301 ggtggttttt tatggaaacc gtaattgcgt ttatcattat atttggctcc ctcgtctttt
     9361 ttcacgaatt aggacatttc ctatttgcta aaaaagcggg gattatggtg cgtgaattcg
     9421 caatcggatt cggaccgaaa atacttgcca tccgtaaaaa agagacgtta tacacaatcc
     9481 gccttcttcc gctcggcggc tatgtcagaa tggcgggcga ggatttcgat acggttgaat
     9541 tgcaacccgg ataccgcgtc ggagtcattt tgaatactga cgatgaaatc gaaaaaatct
     9601 atttgaatcg cgatgtctcc aatccgaatg tcttattttt ggaaacggaa tccgctgatt
     9661 tgagtaaagg acttttcatc gaaggatatg acgaagatgg gaaactcgtg aattataagg
     9721 tctcccgtaa tgctgtcatc tacgaaaaag gacaagagac gctgattgcg ccttatgatc
     9781 gacaattcga ttccaagtca ttgggaagtc gggcgatggc gatttttgcc ggccctttat
     9841 tcaacttcat tcttgcattc ttcatctttt tggcgatcgg ccttctgcat ggcatcccga
     9901 caaatgagcc aatcattaaa gaagtcatca gtgacagtcc agcatatgcc gcgggaatgg
     9961 aagcagggga cttcgtcaag gaagtcgatg gaaagccggt ggaaacttgg caggaattta
    10021 ccgaaacgat acaggcaaat cccgaaaacc gtcttcagat gaaggtcgaa agagacggtg
    10081 aaatggtgac gttggcagtt gttcccgaga aagcggtaaa agaaggaagg gaattcggtc
    10141 agatcggtgt catgtatgaa agtccagttg aaaagaatgt gcttaaagca tttgcctttg
    10201 gtgctgagca gacatttagc tggatgaaaa ggatattcga acttcttggc atgcttgtca
    10261 ccggcaagtt tacaattgac gcgctaagcg gacctgtcgg catctataaa gcgacggagg
    10321 aagtagcgca atggggtgtg ttttccttaa tgaactgggc ggccgtattg agcatcaatc
    10381 taggaatcat gaaccttttg ccgctgcctg cattggacgg cggacgattg ctgttcttcg
    10441 catttgaagg agtgcgcggc aaacctgtcg acaaacagaa ggaaggcatg gtccattttg
    10501 ttggtatcat gttgttaatg gtcctcatgc tcgtcgtcac ctggaacgac atccaacgtt
    10561 tcttctttta atacaacagg ctaaatttca gaaattcgag gtgaaatctt catgaaacaa
    10621 tcaaggacat tcatcccgac aatgcgagaa accccaacgg atgccgatat cctctcccat
    10681 caactcctgc ttagggcagg ctacatccgt cagcatatga gcggtgtcta ttccttcctt
    10741 ccgcttgcga agaaagtgat ccgaaaaatc gaaaacatca tccgggaaga gatggagaaa
    10801 atcgacgctg ttgaaatgtt catgccgacg ttgcagtcgt ccggactttg gaaagagact
    10861 ggcagattgg attcgtacgg tgccgaactt ttcagaatca aggatcgtca tgagcgtcaa
    10921 atggtattag gacctactca tgaagaagtg gttacatcgc tcatccggga tgaagtgaaa
    10981 tcctataaga aactgccgct cacagtctat caaatccaga cgaaattcag ggatgagaaa
    11041 cggccccgat tcggattgct tcgcggcagg gaatttatca tgaaggatgc ttattcattc
    11101 cattcaacag aagagagtct ggaagataaa tataatgaaa tgatgcaggc gtactcgaat
    11161 atcttttcgc ggctcgactt ggatttccgg gcaatcattg ccgatggcgg aacgatcggg
    11221 ggcgaaggaa gccatgagtt catggcagtg tccgatatcg gggaagatac gatcgcttat
    11281 agcgacgctt cctcatatgc cgccaatatt gagatggcag aagtgaaagc ggagtatgaa
    11341 aaatctaaag agacattgaa agaacttacg aaagtcgaaa caccaggaca gaggacgatt
    11401 gcggaagtgt cggactacct gaacgtcgac ccttctagag tgattaagac acttattttc
    11461 aaagcggacg ataagttggt tgtcgtgctg acccgcggcg atcatgacat caacgaaatt
    11521 aagctgaaaa atgctttgca ggcagatgag attgaattgg cgacggaaga cgaaattgaa
    11581 gaaatgcttt cgtgcaaagt tggttccatc ggtcctgtca aactgccgct tgatttgaaa
    11641 gtatatgccg attatggtgt cgagtatatt gtgaacggag tttgcggtgc taacgaagat
    11701 ggctggcatt tggtgaacgt taaccctgag cgcgatttcg ccattgagca gtacttcgat
    11761 ttgaggttca tcaaagaggg cgatccctcg cctgatggaa acggttcaat tcaatttaca
    11821 aaaggaatcg aacttggtca tgtttttaaa ttgggaacca cttatagtgg tccgatgaaa
    11881 gcgacctacc ttgatgaaaa cggcaaggca caaccatata ttatgggctg ctatggtatt
    11941 ggtgtttcac ggatattggc tgcagttgca gaacagcata atgatgccaa tggattgaaa
    12001 tggccgaaac agctctctcc ttatgacatc catcttgtaa caatcaatat gaaggatgaa
    12061 gcgcaaacaa acctatcgga agagctctat catctgctaa aatcataccg gtatgaaatt
    12121 ttacatgatg accgtgatga gaggccgggg gtaaagttca ccgatgcaga tctcattgga
    12181 ttgccaatcc gtttgacaat cggcaagaaa gcggatgagg gcatcgttga agtaagattc
    12241 agatcaaccg gcgaaacagt cgagtggcaa aaagaagaag tgaccgaaaa gctgcaagcc
    12301 tttttcggtt gatgataaaa gaaagagggg gaggccatga ccggcacttc ccttcttttc
    12361 actgtacgaa acgggggagg tatacatatg gatgcaaaag tgaaattgct gacgttgttg
    12421 cagcagattg gtatgactga cgatcaaaca attgcccatt tcgaacatgg aacactggaa
    12481 cgtgtggata ttcatcgtaa atcgagagta tggcaattca acgtggaact tgaaaagaca
    12541 ttgcctgtcc aagtctatca gttattccaa caaaggatgt cggaggcatt tacgtccata
    12601 gcttcagtac gccttcaatt gaaagtgcga aatacagagg gggatcctga acttattgtc
    12661 ggttattggc cgcatgtcat tgaggaaatg agagatatgt cccctccgat ccgtgaaagg
    12721 ctcatcgggc aacagcccgt cctcgttggc gggaaaatga cattgacttg tgcgaatgac
    12781 tttgaacagc aaacgatgaa aaataaatat gggtcgctta tcgccgaggt ttatgaatca
    12841 ttcggctttc cttcgatggt cgtcgaattt gcaatctcgg aagaggctga cgataacgaa
    12901 gcagaaatac ttgcttttga agcgcaaaaa aaggcggaag aagaagagta cgcgaagaac
    12961 gccttagcca acctgcaaaa aatggaagcg atgaagaagg agaatgggga cgctgggaac
    13021 aggccattcg ttttaggggt cccgatcaag cctgatgaac cgatcatcga cattcagacg
    13081 atacaggatg aggaaagacg cgtgacaatc gaaggctatg tattcgatgc ggaagtaagg
    13141 gaattaagaa gcggacgttc cttgctaaca atcaaggtga ccgactacac cgattccatt
    13201 cttgtgaaaa tgttctcaag ggacaatgaa gatgccgagc tcatgaaaac attgaaaaaa
    13261 ggggcttggg tgcgtgcgag gggcagcatt cagaacgata
//
LOCUS       DASULI010000352         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_944423, whole
            genome shotgun sequence.
ACCESSION   DASULI010000352 DASULI010000000
VERSION     DASULI010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944423"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /gene="plsY"
                     /locus_tag="VFX34_07565"
     CDS             complement(<1..397)
                     /gene="plsY"
                     /locus_tag="VFX34_07565"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX5564801.1"
                     /translation="MEIIILILIAYLLGSIPSALWVGKLFYKTDVRQHGSGNLGATNT
                     FRVLGKTAGLTVTILDILKGTAAVLLLLLPSFGSAGIHPLLLGVCAVIGHMFPIFANF
                     KGGKAVATSGGVLLGYNWPIFIIVLLTFLA"
     gene            652..>2471
                     /gene="parE"
                     /locus_tag="VFX34_07570"
     CDS             652..>2471
                     /gene="parE"
                     /locus_tag="VFX34_07570"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX5564802.1"
                     /translation="MTKKEEIYTYDDDSIQVLEGLDAVRKRPGMYIGSTDSRGLHHLV
                     YEIVDNAVDEALAGYGSEIDVTIHKDGSIGVRDYGRGMPTGMHKTGKPTVEVILTVLH
                     AGGKFGQGGYKTSGGLHGVGASVVNALSEWLEVTIFRDGKKFRQRFESGGKPVTTLEE
                     IGTTREKGTLIHFKPDPAIFSTIKYQYELLSERLRESAFLLKGLKIDLKEEGTEKHDV
                     FHYETGIEAFISYLNEEKDVLHDVAYLEGVSEGIEVEFAFQFSDGYSETILSFVNNVR
                     TKDGGTHETGAKTAMTRVFNEYARKTGILKEKDKNLDGSDIREGISAIISVRIPEEIL
                     QFEGQTKGKLGTSEARTATDAIISQHLLYFLEENAELSGNLIRKAIRAQQAREAARKA
                     REDARSGKKRRRSDTLLTGKLTPAQSRNAAKNELYLVEGDSAGGSAKQGRDRTFQAIL
                     PLRGKVINTEKAKLEDIMKNEEINTIIHAIGGGVGADFQIDDIAYDKVIIMTDADTDG
                     AHIQVLLLTFFYRYMKPLIEAGKVFIALPPLYKVFKGTGKKEQVEYAWTEADLDQAIK
                     KIGRGYMLQRYKGLGEMNADQLWETTMNPETRTLIRVTIE"
BASE COUNT          752 a          528 c          635 g          556 t
ORIGIN      
        1 tagcgaggaa cgtcagcaga acgatgatga aaattggcca gttatatccg agcaaaactc
       61 cgccggacgt ggcaacagcc ttaccgcctt tgaagttagc aaagataggg aacatatgac
      121 caattacggc acaaacccca agcagaagcg gatggatgcc tgccgaaccg aatgacggca
      181 aaagaagaag caatacagca gccgtccctt ttagaatatc aagaatcgtg acggtcagtc
      241 ccgctgtttt ccccagaacc ctgaacgtgt tcgtggcacc gaggtttccg ctgccatgct
      301 gccggacatc cgttttgtag aacagtttgc cgacccataa tgcggaggga atcgacccga
      361 gtaaataagc gatgagtata agaatgataa tttccatgac gtaccccttt atcattaatg
      421 aatagttatg tgtttcaagt ttagcagaac agacgaatgc cgacaatgga atgtacaagt
      481 tctgcagata agtcaattgt ctagattttc ctcatctcat tgcaatcagc cggtttcttc
      541 tatacaatta attgagcgtg cttcctgaaa cgttgatttg acaagcttta caggaagaga
      601 tacgatagta tacaaagaga aagagaacat tcgtttgatg ggggttgcga tttgacgaag
      661 aaggaagaga tttacacata tgatgatgat tcgattcaag tgctggaagg ccttgatgct
      721 gtacggaaac gcccggggat gtatatcggt tccacggatt cccgcggact tcatcatctc
      781 gtctatgaaa tcgtcgataa cgcagttgac gaagcgcttg caggatatgg ttcggaaatt
      841 gacgttacga tacacaaaga tggcagcata ggtgtgcgcg actatggacg cggaatgcca
      901 accggaatgc ataagactgg aaaaccgacg gtggaagtca ttttgacagt ccttcacgct
      961 ggcggaaagt ttgggcaagg aggttataag acgagtggcg gcttgcatgg cgtaggcgca
     1021 tcagtcgtca atgccctttc cgagtggttg gaagtgacga tttttcgcga tggaaagaaa
     1081 ttcaggcaac gtttcgaaag tggcggcaaa ccggtcacta cattagaaga aatcggaacg
     1141 acccgtgaga aagggacatt gatccatttc aaaccggacc cggctatttt ctcgacgatt
     1201 aaataccaat atgaattgtt atcggagcga ttacgcgaat ccgcattcct tctgaaaggc
     1261 ttgaaaatcg acctgaaaga agaaggaact gaaaagcatg atgtattcca ctatgaaaca
     1321 ggcatcgagg cattcatctc ctatttgaat gaagagaagg atgtcctcca tgatgtcgca
     1381 tatcttgaag gagtgtccga agggattgaa gtggagttcg catttcaatt cagtgacggc
     1441 tattccgaaa caatcctttc gtttgtcaat aatgtgcgga cgaaagatgg cggtacacat
     1501 gaaaccggtg ccaaaactgc catgacgcgc gtattcaatg agtatgcaag aaaaaccggc
     1561 atattgaaag aaaaagataa aaacctcgat ggctccgata tccgggaagg catttccgca
     1621 atcatttcgg tccgcatccc tgaagagatc cttcaattcg aaggtcagac gaaagggaaa
     1681 ttggggacga gtgaagcaag gacggcaact gatgcgatca tctcccagca tctcctatat
     1741 tttttggaag agaatgcgga gctaagcgga aacttgattc gaaaagcgat ccgtgcccaa
     1801 caggcaaggg aagctgcgcg caaggctcgg gaagatgcgc ggtcaggaaa aaaacgccgt
     1861 cgttcagata ccctgcttac agggaagctg acaccggcac aatccaggaa tgccgcaaaa
     1921 aatgaacttt acctagtcga aggggattcc gccggcggtt cagccaagca aggccgggac
     1981 agaacgttcc aggcaatatt gcctctgcgc ggtaaagtaa tcaataccga aaaagccaaa
     2041 ttagaagata ttatgaaaaa cgaagagatc aatacgatta tccatgcaat cggcggaggg
     2101 gtcggcgctg atttccaaat cgacgatatc gcctacgata aagtgatcat catgacggat
     2161 gccgatacgg acggggcgca tatccaagtg cttctcctta cgttcttcta tcgatatatg
     2221 aagccattga tcgaggctgg taaagtgttt atcgcacttc cgccacttta caaagtcttc
     2281 aaagggacag gcaagaaaga acaagtcgaa tatgcgtgga cggaagccga tcttgaccag
     2341 gcgatcaaaa agatcggccg aggctatatg ctgcagcgct ataaaggtct tggtgaaatg
     2401 aatgccgatc aattatggga gacgacgatg aatcctgaaa ctcgtacgct aattcgtgta
     2461 acgatcgagg a
//
LOCUS       DASULI010000353         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1917533,
            whole genome shotgun sequence.
ACCESSION   DASULI010000353 DASULI010000000
VERSION     DASULI010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917533"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1769)
                     /locus_tag="VFX34_07575"
     CDS             complement(129..1769)
                     /locus_tag="VFX34_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564803.1"
                     /translation="MHEFRTLAILSRFRRLFIKAGIDYDAMETILRMKLTMDGRRTPT
                     IFNGQKKKEGNQFLKSLWIYGLYGLMVIPFLLFGDGHYIFQMSLVFGIIMFILATTMV
                     SDFSSVLLDVRDKPILQTKPVSSKTLNTAKTVHILIYMFFITGSFIAIPLVVGLFAKG
                     ILFTLVFLAALVLTMLFIVVITSLVYFAVLRFFDGEKLKDIINYVQILLSVGVIVGYQ
                     IVIRAFDFVDLDLTYTFSWWHLFLPPIWFGAAFELLLNQDLSNVLILLSSLALLVPII
                     SMYVYVRLMPSFERNMDKLMSESKNKRKKSSWWTDVWAKLCCRSREERTFFRFGALMM
                     QQEREFKLKVYPSLGFSMVFPFIFIFMELQTRTLADISTGKMFLLIYFGNMMIPTIVH
                     TMQFSVTHKGSWLFKAAPIQQPAAAYSGTLKAFLVKLYMPIFILLSIVFIVVFSWRIV
                     PDLFAVLFGSILQTMLTYKWLNSGAFPFTESFDFAQSEGSAKLILLTLLAGVFVIGHL
                     IASAITGGVYVYIAVLVIAIFIGWRKVFPMNGDVPVRL"
     gene            complement(1762..>2123)
                     /locus_tag="VFX34_07580"
     CDS             complement(1762..>2123)
                     /locus_tag="VFX34_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564804.1"
                     /translation="VLIISSLLHNPDLLFFDEPLSGLDANSMMVIREMLAQLAAQGKT
                     IFYSSHIMDVVEKISNRIVLLVKGTVVADGSFEELQEMSEEGSLSDIFNQLTGFTDHA
                     SRAERFISIVEGGTADA"
BASE COUNT          639 a          533 c          443 g          508 t
ORIGIN      
        1 tttcctcctc atttaccaca gactaattat aaggggaaat cgctaagttg cctacatgta
       61 ttctaaattg atcaagttat ctaaattgca tatcgggaat caatgaaaac tggtatttgt
      121 actccaactc acaaccgaac tggcacgtcc ccgttcatcg gaaacacctt ccgccagccg
      181 atgaatatcg cgatgaccag tacagcgata tatacgtaaa ccccacctgt tatcgcactt
      241 gcgatcaggt ggcctatgac aaaaacccct gccagcagag tgagcagaat cagtttagct
      301 gagccttccg attgtgcaaa atcgaaggat tcggtgaacg gaaaggcgcc tgaattgagc
      361 catttatacg taagcatcgt ctgaaggatg gatccgaata aaaccgcgaa caggtcggga
      421 acgattcgcc atgaaaaaac cacgatgaaa acaatcgaca gcaagatgaa aatcggcatg
      481 tacagcttca ctaaaaacgc ctttaacgtc ccactatatg ccgcagcagg ttgctggatc
      541 ggcgctgcct tgaacagcca actcccttta tgtgtcactg aaaactgcat cgtatggacg
      601 atggtcggga tcatcatatt tccaaaataa atgagcaaga acattttccc cgtagagata
      661 tccgctaatg tcctcgtttg cagttccata aagatgaaaa tgaacggaaa taccattgaa
      721 aagccaagcg aaggatacac tttcagcttg aattcccgct cctgctgcat catcaaggct
      781 ccaaaacgga agaaggttct ctcttccctg ctccggcagc aaagcttcgc ccaaacatcc
      841 gtccaccagc tcgatttctt ccgcttattc tttgactcgc tcatcagctt atccatattg
      901 cgttcgaacg acggcatgag ccgcacgtag acatacatgg agatgatggg gacaagtaaa
      961 gcgagtgacg acaacagaat cagcacattt gacaagtctt gattcaacaa cagttcaaat
     1021 gcggccccaa accatatcgg cggtaaaaac agatgccacc aactgaatgt gtacgtcaaa
     1081 tccaaatcga caaaatcaaa ggcgcggatg acaatctgat agccgacgat gacgccgacg
     1141 gatagcagaa tctgcacgta attgataata tccttcaatt tttcgccgtc aaagaaccgt
     1201 aggaccgcaa aatagacaag tgacgtaatc acaacgataa agagcattgt caacaccaat
     1261 gccgcaagaa atactagggt aaatagaatt ccttttgcaa acaacccgac gacgagtggt
     1321 atggcaatga aggaccctgt tatgaaaaac atataaatta gtatatggac ggttttcgca
     1381 gtattgagcg tcttactgct caccggtttc gtctgcaaaa ttggcttatc ccttacatcc
     1441 aacaagacag aagagaagtc ggacaccatt gttgtcgcca aaatgaacat gataattcca
     1501 aagacgaggc tcatctggaa aatataatga ccgtccccga agagaaggaa cggtatcacc
     1561 atgagcccat acaaaccgta gatccataaa gacttcagga actgattgcc ttcctttttc
     1621 ttctgcccgt tgaaaatggt cggcgttcgt ctgccatcca ttgtcagctt catgcgcaaa
     1681 atggtttcca ttgcatcgta atcaatgcct gcttttatga atagccttct gaatctggag
     1741 aggatcgcca atgtccggaa ttcatgcatc ggcagttccg ccctctacga tagatatgaa
     1801 tcgctccgct ctactcgcat gatccgtgaa ccctgtcagc tggttgaata tatcggaaag
     1861 cgaaccctct tcactcattt cctgcaattc ctcgaagctg ccatccgcta cgacagtccc
     1921 tttgacgagc agcacgatcc ggttgctgat cttctcgacg acatccatga tatgtgagga
     1981 ataaaagatc gtttttcctt gcgccgccag ttgcgcgagc atttcacgga tgaccatcat
     2041 gctgttcgcg tccaacccgc ttaacggttc atcgaagaat aatagatcag gattatgcag
     2101 caagctcgaa atgatgagca cct
//
LOCUS       DASULI010000354         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1622160,
            whole genome shotgun sequence.
ACCESSION   DASULI010000354 DASULI010000000
VERSION     DASULI010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622160"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFX34_07585"
     CDS             complement(<1..240)
                     /locus_tag="VFX34_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564805.1"
                     /translation="MSEKRKNRIDLIEEKFESLKKKGWAKGIRITSGVIWNLFLLFLI
                     FGLTLGVFAASVGAGYFASLVAKEPLRSKAEMRGNV"
     gene            605..1870
                     /gene="tyrS"
                     /locus_tag="VFX34_07590"
     CDS             605..1870
                     /gene="tyrS"
                     /locus_tag="VFX34_07590"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5564806.1"
                     /translation="MKNELLEDLKWRGLLYQLTDEEGLEKVLNEEKISLYCGTDPTAD
                     SLHIGHIVPFLTLRRFQQHGHRPIVLVGGATGMIGDPSFKAEERQLLSDEQIAKNVAG
                     IRRQLEHIFDFESENGAILVDNKDWFGEMSAISFLRDYGKLLSINYMLAKENVASRLE
                     TGISFTEFSYMLIQGADFSHLYDKYKCRLQIGGSDQWGNITTGLEIIRKTHSEEAKAF
                     GITMPLITKADGTKFGKTAGGSVWLDPAKTSPYEFYQFWINTADAEVINYLKIFTFMS
                     REEIEALEASVQDEPHLRKAQHALAEEMTRLIHGEDALDQAIRISKALFSGDLKALTV
                     AEMKDAFKDVPTAELDKADKNIVDFIVEAGVSSSKRQAREDVTNGAISLNGEKVTDLA
                     YDVSAADRLDDAFTIVRRGKKNYKMVKFI"
     gene            complement(2039..>2542)
                     /locus_tag="VFX34_07595"
     CDS             complement(2039..>2542)
                     /locus_tag="VFX34_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5564807.1"
                     /translation="RSYFMAPDANGGKAYALLRKALKESGKIGVAKIIIRSKEQLAVV
                     RVYKDALLMETIHFPDEVRNVGDVPNIPAEDTVVKKELDTALLLVDQLTTAFDPDKYT
                     DEYRTALMELIEEKKAGNAAVAKTEKKAISPSNTTDLMAALQASLDKTKKKKSAPRKR
                     TAAKMKA"
BASE COUNT          676 a          579 c          624 g          663 t
ORIGIN      
        1 tacatttccc cgcatttccg ctttggatcg gagaggttcc ttcgctacga gcgatgcgaa
       61 ataaccggcg ccgactgagg cagcaaaaac gccgagcgtc agcccgaaga taaggaagag
      121 aagaaagagg ttccagatga ctccggatgt gatgcggatt ccctttgccc atcctttttt
      181 cttcaatgat tcaaattttt cctctataag atctatcctg tttttacgtt tttcactcaa
      241 tgtttcatcc ccccctaaaa tccttcctat tatagcacat ttcatggaaa tcaatagaaa
      301 atgtttgaca aagcgatagt ttagactaca ataagggata tatcattttt cacatggtga
      361 gaaagccgga gtaggaacgg tcgagccgtc tagagaggca gcggttggtg gaagctgtcc
      421 ggtagaacgt ttacgaatta cagctttgga gtgtgacgcg tacgcggcgt taatgcgtct
      481 tcaagcggag aatgcatata ttctcaagcc gggtggtacc gcgttagttg actttatatc
      541 taacgtccct gcatgattca attgaatcgt gtaggggctt tttcaattta caggagggat
      601 tttgatgaag aatgaactgc tcgaggattt gaaatggaga ggcctgctgt atcagctgac
      661 tgatgaggaa ggccttgaga aggtattgaa tgaggagaaa atctcattgt attgcggaac
      721 agaccctacg gcggacagct tgcatatcgg tcatattgta ccatttttga cacttcgccg
      781 ttttcaacag cacggccacc gtcccatcgt tctggtgggc ggagccaccg gcatgatcgg
      841 ggatccgtcc ttcaaggcgg aagagcgaca gcttctatca gatgagcaaa ttgcgaaaaa
      901 cgtggcgggc atccgccggc aattagagca catctttgat tttgaaagtg aaaacggagc
      961 tatcttagtc gacaataaag actggtttgg ggaaatgtct gccatcagct tcctccgtga
     1021 ctacggaaag ctgttaagca tcaactatat gctcgcaaaa gagaatgttg cttcccgtct
     1081 cgaaacaggc atctccttca cggaattttc gtatatgctg atccaaggcg cggacttcag
     1141 ccacttgtac gacaagtaca aatgccgttt gcaaatcggc ggctccgatc aatggggcaa
     1201 tattacaaca ggacttgaaa tcatcagaaa aacacatagt gaagaagcga aagcattcgg
     1261 aatcacaatg ccgctcataa cgaaagcgga tggcacgaaa ttcggaaaaa ctgccggcgg
     1321 ttccgtctgg ctggatccgg caaagacgtc gccttatgag ttctatcagt tctggatcaa
     1381 cacagcagat gcggaagtca tcaactactt aaaaatcttt acattcatga gccgggaaga
     1441 gattgaagcg cttgaggcat ccgttcagga tgagccacat cttcgcaaag cccagcacgc
     1501 gctggcggaa gaaatgacgc gtctcatcca cggcgaggat gcgctcgacc aagcaatccg
     1561 tatttcgaaa gcgctattca gcggtgactt gaaggcgttg acggttgccg aaatgaaaga
     1621 tgcattcaaa gacgtgccga cagctgaact tgataaggcg gataaaaaca tcgtcgactt
     1681 catcgtcgaa gcaggtgttt cttcttccaa acgccaagcc cgtgaagacg tgacaaacgg
     1741 cgccatttca ttgaacggcg aaaaggtgac ggatcttgca tatgatgtct ctgcagcaga
     1801 ccgcttggat gacgcattca caatcgtacg cagagggaag aaaaactata aaatggtgaa
     1861 attcatctaa tacaaaaatg ccatccttca agggatggca tttttgtatg tggaaaaggg
     1921 ggagtcggtg gtaaatggcc aagagtcggt ggtaaacttt gcgaatcggt ggtaaagtgc
     1981 tccgagtcgg tggtaaaccc tttgaagtcg gtgataacga atgccacgca cgattttttc
     2041 acgctttcat cttcgctgcc gtacgtttcc gtggtgcaga tttcttcttt ttcgtcttat
     2101 cgagcgatgc ttgcaatgcc gccatcaaat cggttgtatt cgaaggtgaa attgcctttt
     2161 tctccgtttt ggcaacagct gcattgcccg cttttttctc ttcaattaat tccataagag
     2221 ctgttctgta ctcatccgta tatttatcgg ggtcgaatgc agtcgtcaat tgatcgacta
     2281 acagaagcgc tgtgtcgagc tcttttttca cgactgtatc ctcagcggga atattcggta
     2341 catcccctac attacgaact tcatccggga agtggatcgt ttccatcaat aaagcgtctt
     2401 tatatacccg gacgactgct agctgctcct tcgaacggat gatgattttt gcgacaccga
     2461 tttttccgga ttccttcaac gcctttcgca atagcgcata cgctttgccg ccgttcgcat
     2521 caggagccat gaagtaactg cg
//
LOCUS       DASULI010000355         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1236243,
            whole genome shotgun sequence.
ACCESSION   DASULI010000355 DASULI010000000
VERSION     DASULI010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236243"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..474
                     /locus_tag="VFX34_07600"
     CDS             154..474
                     /locus_tag="VFX34_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564808.1"
                     /translation="MAQQLLTATSRTEEEHRDAVIDDINALLDERDALQTEIAAPFTP
                     EEEELGKQLVALEKQVAAALASYSKMIRSDITAAQSKKEHVKSYVNPYGKVARDGTFY
                     DTKQ"
     gene            596..2062
                     /locus_tag="VFX34_07605"
     CDS             596..2062
                     /locus_tag="VFX34_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5564809.1"
                     /translation="MNIDQLLKQDWLQKNRIMMIGFGIAGGLGLLAQLIQQSPLAIIL
                     SVALPVALALLYYFSSVKWERLSPFVPYVLLLLTFSVALGVIYFSEANLGSIGIILLI
                     LALASIHGKMPIMGFGLALGFTALVLNNINFVEPELVTGSGTNLLLLFFLAGIVLFLV
                     VRQNGKVFLKVEELMALTEAKVHEEERLKNNMEQAVGTITDYLSQLRTSSESSSASQR
                     EMLAAINEVSVGSQHQADHISDIADEAERTNDVVGSISSGFESIVSQSENAGVQAEKG
                     AVNMSDLKKNMDLFSESFKDLNETFRALTAKITETNEFATSIQAITDQTNLLALNASI
                     EAARAGEFGKGFAVVAEEIRKLAGMTDQTLHKINQNLHDVNEYNELAVSKMEEGLGQL
                     TVQSTVAEESMHSFNSLYTAMDKLKVNLTSFNDEFQTISQNSERIQQRTMDFASIVQQ
                     STAAIEELNATLTDSVDEQQRVASYIQKTHEEAIRLTE"
     gene            2180..3661
                     /locus_tag="VFX34_07610"
     CDS             2180..3661
                     /locus_tag="VFX34_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5564810.1"
                     /translation="MGAEHVDKLMQEQFAKKNFILFITLGASSLIGAIYYFLTGQDAL
                     KTISMTIPMTTSIVLYLLSRKFVFFEKLFPWVIIGITAAAAIFNGIVGDPSIATAGIA
                     FFIAGIASVHISMRIMSYGFVLSVLVLIVFLTKYPYQEQIASSKGSMVLPLILMAVGL
                     LIQIRQTKKLESRVNQFTAEQAERAMEEERKHRSLNENVEQVADDLMSISTTAEQHLA
                     SQQELLGIMNTLAAGVEQEAEQIGRIAQNAERTQSDVLDMQNETRTMVAEASELRKES
                     DEVVGVMRDVRTGMEEVEQLLNELNGSFGALTQNIERTNALAKSIATITEQTNLLALN
                     ASIEAARAGDHGKGFAVVAEEIRKLANMTAETLTEINGNLNDVNTMNGRSQENLAAST
                     GKLKSQGAMTVQAEKKIEQMHQTLDGLHHKFELFDSKMNVIAEETSEIGEMTGTFADL
                     LAQSSASLEEVNATVHSTVSDNEQIVRTLDGTMRRTKELAEVR"
BASE COUNT         1063 a          821 c         1022 g          920 t
ORIGIN      
        1 cattaaaaac gacctgacgc tctacgatga agccgcagga atcataacgg agtttcgcga
       61 tacgtggaag caagtgatcc agatcaatcg cacgaagcag tacgcgaaca cggacgaaat
      121 atgatccggc cggccatgat ccggtggcag gagatggcgc agcaacttct gaccgcgaca
      181 agtcgtaccg aagaagagca tcgggacgcc gtcatcgatg acatcaacgc gttgctcgat
      241 gaacgggacg ccttgcagac ggaaatcgcc gcacctttca caccggaaga agaggaattg
      301 ggcaagcagc ttgtcgctct cgaaaaacaa gtcgctgcgg cgcttgcctc ctattcaaaa
      361 atgatccgtt ccgacatcac cgccgcccag tcaaagaaag aacatgtcaa aagctatgtc
      421 aatccttatg gcaaggtggc gcgggatgga acgttctacg atacaaagca ataatacgcc
      481 aatcaggtag gcctggttgg ttttttcggg tatgattcca ttactcatgc agtatgaatg
      541 attactcatt catctttatt ttcacactat tttcttatcg cttgggggtt ttttgatgaa
      601 tattgatcag ttactgaaac aggattggct tcaaaagaac cggattatga tgattggatt
      661 tgggattgcg ggtgggctgg ggttgcttgc gcagctcatt cagcaatcgc cgcttgccat
      721 cattctatcc gtcgccttgc cagttgcgct cgcgcttcta tattattttt caagtgtgaa
      781 atgggaaaga ctttcccctt tcgttcctta tgtattgctc ctattgactt tctcggtagc
      841 gctcggtgtc atttattttt cggaagcgaa tttgggatcc atcggaatca ttttgctcat
      901 attagcccta gcctcgatcc atggcaaaat gccgatcatg gggttcggat tagcgctggg
      961 cttcaccgca ctcgtattga acaatatcaa tttcgttgaa cccgagcttg tcactggaag
     1021 cgggacgaac ctgcttttat tattcttcct cgctggcatt gttttgtttt tagttgtgcg
     1081 gcagaatggc aaggtgtttt tgaaagttga agagctgatg gcgttgacgg aagcgaaagt
     1141 ccatgaagaa gagcgtttga aaaataatat ggaacaagct gtcggaacga ttacggacta
     1201 tctttcccag cttcgaacca gttcagaatc gtcttccgcc tcccaacgtg aaatgcttgc
     1261 ggcaatcaat gaagtgagtg taggcagtca gcatcaggcg gatcacattt ccgacatcgc
     1321 cgatgaggcg gaacggacaa atgatgtggt tggcagcatc tcaagcggtt ttgaaagcat
     1381 cgtaagccag tcggaaaatg caggtgttca agcggaaaaa ggcgctgtaa acatgtctga
     1441 cctgaaaaag aatatggatc tgttctcgga atctttcaag gatctgaatg aaaccttccg
     1501 ggcattgacg gcaaagatta cggagacgaa tgaatttgca acatccattc aagctattac
     1561 ggaccagacg aatttattgg cgctgaatgc ttcgatcgag gcagcccgcg caggtgaatt
     1621 cggaaaagga tttgcggttg tcgcggagga gatccggaag cttgcgggca tgacggacca
     1681 aacccttcat aagattaatc agaacttgca tgatgtcaat gaatacaacg aattagccgt
     1741 ctcgaaaatg gaggagggtc ttggacagtt gacagtccag tcgactgtcg ctgaggaatc
     1801 gatgcattcc ttcaattctc tatacacggc aatggataag ttgaaagtga atctgacgtc
     1861 tttcaatgat gaattccaga cgatttcgca aaacagcgaa aggatccagc agcggacgat
     1921 ggatttcgcg tcgatcgtgc agcaaagcac tgctgcaatc gaagagttga atgcgacgtt
     1981 gacggattcg gtcgatgagc agcaaagagt ggcttcgtat attcagaaga cgcatgaaga
     2041 agcgattcgc ttaacggaat aaagatagat gccccggagt tcttcggggc tattttttta
     2101 gatggaacta ttttactatt catgatacat tgaaaataga gacatacagt taacaaaaaa
     2161 aggcaagggg gaaagtgaaa tgggagcaga acatgtggat aaattaatgc aggagcaatt
     2221 tgcgaagaag aatttcatat tattcattac actcggtgca tccagcttga tcggagccat
     2281 ctattatttt ttgaccgggc aggatgcgtt gaaaacgatc agcatgacga taccgatgac
     2341 gacttcaatt gtcctgtatc ttttatcacg gaagttcgtg ttttttgaaa agctttttcc
     2401 atgggtcatc atcggaatta cggcagctgc cgccattttc aatggaattg tcggcgaccc
     2461 atccatcgcg accgcaggca ttgcgttctt cattgcagga atcgcaagcg ttcatatatc
     2521 gatgcggatc atgtcatacg gcttcgtctt gtccgttcta gtactgattg tgttcttgac
     2581 gaaatatcct tatcaggaac agatagcgtc aagcaaaggg agcatggttc tcccgctcat
     2641 cctcatggcc gtcgggctgc tgatccaaat caggcagacg aagaagctgg agtcacgtgt
     2701 caatcaattc acggcggagc aggcggaacg ggccatggaa gaggaacgga agcaccgcag
     2761 cttgaatgaa aacgtcgaac aagtggcgga cgatctgatg tcgatcagca cgaccgctga
     2821 acagcatctc gcttcccaac aggaactgct tggcatcatg aatacccttg cggcaggtgt
     2881 ggagcaggaa gccgagcaga tcggcaggat tgcacaaaat gccgaacgga cacagagcga
     2941 tgtgctggat atgcagaacg agacccgcac gatggttgcg gaagcctcgg agttgcgcaa
     3001 ggagtccgat gaagttgttg gcgtgatgcg cgatgtgcgg acgggcatgg aagaagtcga
     3061 gcagttgttg aatgaactaa atggttcatt cggcgcactt acacaaaata tcgagcggac
     3121 aaatgcgctt gcaaaatcga ttgcgacgat cactgagcag acgaatctac tcgctctgaa
     3181 tgcgtcgatc gaggcggccc gcgcaggcga tcatggaaaa gggtttgcag tcgttgcgga
     3241 ggaaatccga aagctagcca atatgacagc cgagacgttg acggaaatca acggcaactt
     3301 gaatgacgtc aatacgatga acggccggtc gcaggaaaat ttggcggcaa gcacagggaa
     3361 attgaaatcg caaggcgcga tgaccgttca ggcggaaaag aaaattgaac agatgcatca
     3421 aaccttggat gggctgcatc ataagtttga actgtttgat tccaaaatga acgtcattgc
     3481 ggaagagacg tccgaaatcg gggaaatgac aggcacattt gcggatctgc tggcgcaatc
     3541 aagtgcatcc ctggaagaag tgaatgcgac cgtccactcg actgtttctg acaacgaaca
     3601 gatcgtcagg acgttggatg gaacgatgag aaggacgaag gaattggctg aagttcgata
     3661 attctcccca gatttggtat actgaatata gggaatagat aaggaacgga gtggtgaaat
     3721 gctatataag cgccatgagt atttcaggca ttcattcggg attccgctga atgcaacttt
     3781 tcggattgtc gtcggtgaag gtgcgacgaa gtccaatcca ggagac
//
LOCUS       DASULI010000356         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1060906,
            whole genome shotgun sequence.
ACCESSION   DASULI010000356 DASULI010000000
VERSION     DASULI010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060906"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..816)
                     /locus_tag="VFX34_07615"
     CDS             complement(241..816)
                     /locus_tag="VFX34_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5564811.1"
                     /translation="MTILSEILTYNKEFVNEKKYEEYATTKFPDKKIVILTCMDTRLT
                     ELLPKAMNLRNGDAKIIKSAGAIVTHPFGSVMRSILISLYELKADEVYIIGHHDCGMS
                     SIDTEQIIDKMVDRGVDKNLFKTLQYSGIDMKDWLHGFSDVTESVKRSVNQVRNHPLM
                     DRKVPVHGLVAHPDTGLLDIIVDGYKAIDEN"
     gene            complement(1173..>2155)
                     /locus_tag="VFX34_07620"
     CDS             complement(1173..>2155)
                     /locus_tag="VFX34_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5564812.1"
                     /translation="VMEREKLAEGGAAFVSVVSQSPDLLKGVDPSRIADAQKASGKAL
                     SNYRKMLQADRFSWSVVAAPSKAWAAKMFPDLGEAEQVPALWEAIFKAVRADQDDAVG
                     AWKELDRNLNEKADYLNEKAYAKLHYKAPGTDLTIELPKGHIWAGGGSVNVDGNAFMA
                     NMPTEEVFTVPHKTGVNGYVKNTKPLSYGGNIIDDFTITFKDGRITEVEAGQGEDVLK
                     HLIDTDEGAKHLGEVALVPHASPISESGLLFYNTLFDENASNHLAIGSAYSFCLEGGK
                     TMSAEELEKHGLNQSITHVDFMIGSAEMDIDGVLEDGTVEPVFRNGNWAF"
BASE COUNT          529 a          497 c          479 g          650 t
ORIGIN      
        1 gaagaattgg tcagactgta gataagcacc tgagattatg gtgtttgtct acagtctttt
       61 ttcaaatgga agtaatgcta gaaatattgc tttccgttcc ggtggaaagc gtccgccgta
      121 atggaaatca gctgctttaa agtgaaaaaa gcggcagtct gaaaagtcat ttcacatgat
      181 cttttgggac tgccactttt ttagatttgt ctatttcccg ggaaatttga aatggtggtg
      241 tcagttttcg tcgattgctt tgtatccgtc gacgatgatg tccaatagac cggtgtcagg
      301 gtgggcgacg aggccgtgta caggcacttt acggtccata agtggatgat tccgcacttg
      361 gttcacactt ctttttacgc tttctgtaac atcactgaag ccgtgcaacc aatctttcat
      421 atcgatgccg gagtattgta aggttttgaa cagatttttg tcgactcccc ggtctaccat
      481 cttatcgatg atctgttccg tgtcgatgga gctcatccca cagtcgtgat ggccgatgat
      541 ataaacttca tccgccttca attcgtaaag ggaaatcaaa atgcttcgca tgacgctgcc
      601 gaatggatgc gttacgatgg caccggcgct cttgatgatc ttcgcgtcgc cattacgtaa
      661 gttcatcgct ttcgggagta attctgtaag tctcgtgtcc atacaagtta agatgacgat
      721 tttcttatcg gggaattttg tagtggcata ttcttcatat ttcttctcgt tgacaaactc
      781 tttattataa gtaagtattt cagacaaaat tgtcatgatg gggccctcca ttgtattggg
      841 atttcatgag ctgaaaaatc tggctgcatt caaactatat taaatagtgt acagaaaaag
      901 aaaagagctg ccaagtggca actctttgtt ttattttact tccggttttg gatcgattgt
      961 ttctgcatcg gaagaattga gagctctgct aagatagtgc attgtaaaat aagctgaaat
     1021 accaaggaca actgcgtacc cgataactgt agtatacatc aagcccatga cgagacccct
     1081 ctctaaaaaa ataattatta tacattcatt atactataat cttgaaataa ttaaagagca
     1141 gattgaaatc tttcaatgaa ttgtgtctaa aatcagaagg cccagttccc attgcggaat
     1201 actggctcga cagttccatc ctccaatacc ccgtcaatat ccatttcggc agagccgatc
     1261 atgaagtcga catgcgtgat gctttggttc aggccatgct tttccaactc ttccgcagac
     1321 atcgtttttc cgccttcaag acagaaggag tatgcgctgc cgatagcaag gtgattcgat
     1381 gcattttcat cgaatagcgt attgtagaag agaagcccgg actctgaaat cggtgaagcg
     1441 tgaggtacga gtgcgacttc tccgagatgc tttgcccctt catccgtgtc gatcagatgt
     1501 ttgagcacat cttcaccttg gccggcttcc acctcggtga tccgtccatc tttgaaagtg
     1561 attgtgaaat catcaatgat attgccgccg tagctgagcg gcttcgtgtt tttgacatag
     1621 ccgtttacgc ctgtcttatg cggtaccgtg aatacttcct cggttggcat atttgccatg
     1681 aatgcatttc catcgacgtt gacacttccg ccgcctgccc aaatatggcc tttcggcaat
     1741 tcgattgtca agtctgtgcc cggtgccttg taatgtaatt tggcatatgc tttttcattc
     1801 aagtaatcgg ctttttcatt aagattgcgg tcaagctcct tccatgcgcc gacagcgtca
     1861 tcctgatcgg cccgaacggc tttgaaaatt gcttcccata gtgccggaac ttgctcggct
     1921 tcacctaagt ccgggaacat ctttgcagcc catgcctttg aaggagcagc cacgacggac
     1981 cagctgaaac ggtctgcctg caacatttta cggtaattgc tgagcgcttt cccgctagcc
     2041 ttttgggcat ctgcaattcg ggaaggatcg acccctttca gcagatccgg actttgtgag
     2101 acgacactaa cgaatgccgc tccgccttcc gccaatttct cgcgttccat cactt
//
LOCUS       DASULI010000357         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_447304, whole
            genome shotgun sequence.
ACCESSION   DASULI010000357 DASULI010000000
VERSION     DASULI010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447304"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VFX34_07625"
     CDS             complement(<1..1420)
                     /locus_tag="VFX34_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HEX5564813.1"
                     /translation="MGNHMDRIRIEKLKDLVVSPEEAASWIKDGMTLGLSGFTRAGDA
                     KAVPMALVKRAETEKFKVNVFTGASLGSDIDKLFAEAGIVKKRLPFQAEKAMRNGINN
                     GELLFVDHHLSHTAEWIRSNVIEPIDFAILEAISVTEDGMIIPSTSVGNSLSFAKHAK
                     SIIIEINTAQSTEWEGIHDLYDPGKQGERNPVPLTKVDDRIGTIGIPIDVDRVKGIVF
                     THQKDSASTITQPDHDTEVMAEHLLSFLRSEVEAGRLTNSLAPLQSGIGSQANAVLHG
                     LLNSEFENLEVYSEVLQDAVFDLIDAGKVKTASCASITLSEEKMDKVFNNLDKYRDKL
                     IMRPQEISNYPEIIRRLGLISINTALELDIYGNVNSTHVTGTKMMNGIGGSGDFARNA
                     RLAIFVTKSIAKNGDISSIVPFVSHVDHTEHDVDVIVTEHGYADLRGLAPRERVGLII
                     ANCAHPMYRGQLWEYYYEALERG"
     gene            complement(1565..2203)
                     /locus_tag="VFX34_07630"
     CDS             complement(1565..2203)
                     /locus_tag="VFX34_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5564814.1"
                     /translation="MLNLDSLLDVVGELFADEISIVVSNTKEYIYYRPSKRIDLKIRP
                     GDPVREGTIAYKALTEGQKVSEFINRDVFGVPYHGMAVPFHDNGVLDGCVVAIYPAYT
                     EGKSVVTVKTEDGWVPVPFSEVKYIEVRDRKTVVVANHITGTHRHSLQNFEFVLPHDL
                     FVRCHRSFIVNVTQIKAIYPDTHSTFTLSMNDGAQIPVSQSYSSYFRKLLGF"
     gene            2345..2974
                     /locus_tag="VFX34_07635"
     CDS             2345..2974
                     /locus_tag="VFX34_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5564815.1"
                     /translation="MHIRDLIVERLGVATVKVDQTVSETLAKINETGYRCIPVVDEED
                     TYKGMIYKVDLLSYLYEEKGDGEKPVDSLLRHQDIYLYENASFLSALLKIKALPFISV
                     VRDGKLLGILTHNKVESVLEDAFGLKTGGINITLASTEARGMIRKLTTTLKDENIEGL
                     ITLDNGSALARRVVITLEDGKSDEEMVKMRERLEKHGFRILQMQRIEPK"
BASE COUNT          836 a          754 c          691 g          808 t
ORIGIN      
        1 ctcccctttc aagcgcttcg tagtagtatt cccatagctg cccacggtac atcgggtgcg
       61 cgcaattggc gatgatcagg ccaacgcgtt cccttggtgc aagtccgcgc agatccgcat
      121 agccgtgctc tgtgacgatg acatcgacgt catgctccgt atggtccaca tgcgacacaa
      181 acggcacgat gcttgaaatg tcgccgtttt tggcgatcga tttcgtgacg aagatggcaa
      241 gtcttgcgtt ccgggcgaaa tcccctgacc cgccgatgcc attcatcatt ttcgtacccg
      301 tgacatgcgt cgaattgacg ttgccataaa tgtcgagttc aagcgccgtg ttgatggaaa
      361 tcaaaccgag gcggcggatg atttccgggt agtttgaaat ctcctgcggt cgcatgatca
      421 gcttatcccg gtatttatcc agattgttaa agaccttgtc catcttctct tccgataatg
      481 taatcgatgc acatgaagcg gttttgactt tacccgcatc aatcaaatcg aatactgcgt
      541 cttgcaatac ttcggaatac acttcaagat tctcgaattc cgagttcaat aatccatgaa
      601 gcaccgcatt tgcctgtgat ccaatacctg attgaagagg tgcaagactg ttcgttaatc
      661 tgcccgcttc cacttccgag cgcaagaatg acagcagatg ttctgccatg acttccgtgt
      721 cgtggtcagg ctgtgtgatt gtagaagcgg aatctttttg gtgcgtgaac acgattccct
      781 tcactcgatc cacgtcgatt gggatgccga tcgttccgat tcggtcatcc actttcgtca
      841 atggaaccgg attccgttcg ccctgtttgc ccgggtcata caggtcatgg atgccttccc
      901 attcggtcga ctgtgccgtg tttatttcaa tgataatcga tttcgcatgc ttcgcaaatg
      961 atagcgagtt gccgacagaa gtcgacggta tgatcattcc gtcctccgtc actgaaatcg
     1021 cttccaaaat tgcaaagtcg atcggttcga tcacatttga acgaatccac tccgccgtat
     1081 gggacaggtg atggtcgaca aaaagaagtt ccccgttgtt aatgccgttg cgcattgcct
     1141 tttccgcttg gaacggcagc ctctttttca caatgcccgc ttccgcgaat aatttatcga
     1201 tatccgagcc gagggatgcc cccgtaaata cgttcacttt gaacttttcc gtttcagcgc
     1261 gcttcaccaa tgccattgga accgctttcg cgtcacctgc acgtgtgaat ccgctcaatc
     1321 ctagagtcat tccgtccttg atccaagaag cagcctcttc aggcgaaacg accaagtctt
     1381 tcaatttttc aattctgata cgatccatat ggttgcccat ttaaatcgcc ccttttcata
     1441 acttttagat aattaaatta taactctttc aagtgctgaa aaggggtttt gtgaatgaaa
     1501 taccggattt gcaaacagac aagctcattt aatgactgaa acagcatatt tttgatttat
     1561 caacttaaaa tcctaacagt ttacgaaaat aactggaata agactgactg acagggattt
     1621 gtgcgccatc attcatggaa agcgtaaacg tcgagtgcgt atccgggtaa atggccttaa
     1681 tttgtgtcac attgacgatg aacgaccggt ggcaacggac aaacaagtca tgcggcagca
     1741 caaattcaaa gttttgcaat gaatgccggt gggtacctgt aatatgattg gcaacgacta
     1801 ccgttttccg atcccgcact tcaatgtact tcacttcgga aaacgggact ggaacccacc
     1861 cgtcctctgt tttcactgtg acgacggact tcccttctgt gtatgccgga tagatggcaa
     1921 cgacgcatcc gtccaaaacg ccattatcat ggaacggaac cgccatgccg tgatacggca
     1981 caccaaacac atcacggttg atgaattcgg agaccttctg tccttccgtt agtgccttat
     2041 aggcgatcgt tccttcccgt acagggtcgc ccggcctgat tttcaaatca atccgcttgc
     2101 tcggccggta ataaatatat tctttcgtat tcgacacgac aatggagatt tcatctgcaa
     2161 acaactcgcc gacaacgtcg aggagtgagt ccaaattcaa caaagtatgt aagccccttt
     2221 cgcgcttcag atactactag tataagtttt catgtacaac attccaattt tcaattataa
     2281 tgggaagcga gataaacaaa aatttcgact tcacagaaac tacagtaaat aagggggagt
     2341 tataatgcac attcgggatt tgattgtgga aagactaggt gtcgctacag taaaggtaga
     2401 tcaaactgta tctgaaacac tagcaaaaat caacgagacg ggataccgtt gcatccctgt
     2461 cgttgacgaa gaagacacgt ataaagggat gatctacaaa gtcgacctgc tttcctatct
     2521 ttacgaggaa aaaggggacg gtgaaaagcc ggtcgactct ttgttgagac atcaggacat
     2581 ctacttatac gagaatgctt cttttttaag cgccttgctt aaaattaaag ctttgccttt
     2641 catcagcgtt gtcagggatg ggaagcttct tggcatcttg acgcataata aagtcgagag
     2701 cgtcctcgaa gacgcattcg gattaaaaac aggcggcatc aatatcacgc tggcatcgac
     2761 cgaagcgcgg ggcatgatca gaaaattgac gacgacgctg aaggacgaaa acatcgaagg
     2821 cttgatcacc ctcgacaacg gatcggcact tgcacgaaga gtcgtcatca cactcgaaga
     2881 cggaaaatcc gacgaagaaa tggtgaaaat gcgcgaaaga ctcgaaaaac acggattccg
     2941 gattctgcag atgcaaagaa tcgaaccaaa ataagatgaa aataaaaggc agtttccaag
     3001 tcgaaatcga caatggaaac tgccttttct tatgcaagtt cgctttccgg cttcggcacc
     3061 atgcgcaaca gaatgcctcc gataataaa
//
LOCUS       DASULI010000358         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1489280,
            whole genome shotgun sequence.
ACCESSION   DASULI010000358 DASULI010000000
VERSION     DASULI010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489280"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..695
                     /locus_tag="VFX34_07640"
     CDS             78..695
                     /locus_tag="VFX34_07640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5564816.1"
                     /translation="MITDKTCLDVGCGHGDFTIKCGSFAKGIVGFDCTDAFIQTGNAN
                     RRSNVTFVVGNAKDGLPFASDEFDCAYNRKGPTSAYPELQRVVKKGGNILGLHPGDDL
                     GKELPTIFPHLFPRCSGTPIADKLTDQLVKSRFTYYRIQTYHAIEYLKTPHDVVLYRC
                     FGQTPELIYEVMETSLPEITRIFNEHATVDGLPITFSRYIVRAIV"
     gene            720..1256
                     /locus_tag="VFX34_07645"
     CDS             720..1256
                     /locus_tag="VFX34_07645"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5564817.1"
                     /translation="MIYIVRHGQTDVNQEGRLQGRVGKPLNSTGFKQAELVKEQLQDI
                     EFDLVFSSPQERARQTAAIITGRPVISDPRIDVYDIGEADGMKKDEVKRAGFIPDPTV
                     YKGIENPSDFVSRVFDFMKELENEYGNQEIKILIAGHRCTTGCIGAYFEGIPEDQNIL
                     RYSSDNGDFKVYQFSKTV"
     gene            complement(1333..2184)
                     /locus_tag="VFX34_07650"
     CDS             complement(1333..2184)
                     /locus_tag="VFX34_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX5564818.1"
                     /translation="MKLFADSACDLPKAYFDENDVTLIPLRVLIGETEYEDLVTINPK
                     EVYKEIRNGQHPKTSQASPELFMELWSELAKSGEKGLYIAFSSELSGTHNTAVMIRDQ
                     IKEEYPDFDLAIIDSKCASLGYGLLVKEAVRLRDAGDDVKTIEQKIRFMAEHMEHLFT
                     VEDLDYLAKGGRVTKASAFIGGLLNIKPLLHVEDGKLVPLEKHRGRKKVFRRITELMA
                     ERGDHLGEQTIAISHGDDVEAALEMKSAIEELFRPKAVELHLIGAAVGSHAGPGTIAV
                     FFLNKLN"
     gene            complement(2213..3022)
                     /locus_tag="VFX34_07655"
     CDS             complement(2213..3022)
                     /locus_tag="VFX34_07655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX5564819.1"
                     /translation="MKPHLIVLDLDGTLLTDEKVISSETKNTLLEAKEAGHQVMIATG
                     RPYRASEIYYRQLGLTTPIVNFNGAFVHHPLDRSWQTIHETIDLHVVKDVVEALEGFE
                     YINLVAEVMDDVYMHYHDEKLLDIFGFGGPNITTGDIRKYLRDHPTSLLVQADEDKVD
                     PIRSHLHEVHAEVIDHRRWGAPWHVIEIVRHGLNKAVGLSHVSKWLDIPRERIIAFGD
                     EDNDLEMIEYAGVGVAMGNAISPLKSIADEVTLSNNENGIAEVLRERLHLG"
     gene            3227..4000
                     /locus_tag="VFX34_07660"
     CDS             3227..4000
                     /locus_tag="VFX34_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5564820.1"
                     /translation="MLVLNETWGALPLLHVVQEEVVEQEVPVVIFLHGFTSAKEHNLH
                     YAYNLAKQGIRVLLPDAHLHGERTEHLDQVQLSLRFWEIVLTSIGEVGEIYNEVKKRG
                     LAPDGKIGVAGTSMGGIETLGCLAAYQWIDVAGVMMGSPGFVQLAKAQMAQFEKRGFK
                     LPITEEERQRVLEAIAVYDLTKKRHALNKRPVHFWHGMQDTVVPFEPTHNFYKALKAD
                     YADVPERLSFTVEEESGHAVSRSGMLEAVDWLARHLNHA"
     gene            4048..>4322
                     /locus_tag="VFX34_07665"
     CDS             4048..>4322
                     /locus_tag="VFX34_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX5564821.1"
                     /translation="MDQDMKDSMMGALENVIDPELGIDIVNLGLIYDVELTEEGKSVV
                     TMTLTSMGCPMGPMIVDNIKTELAELPEVKDVEVNIVWNPPWSKDKMS"
BASE COUNT         1183 a          907 c         1023 g         1209 t
ORIGIN      
        1 gaacatttac atattcatgg gaatcaactg ttactgtgcc gaacggggaa actgtatttg
       61 actcagaggt aaaagacatg attacagaca aaacatgctt ggatgtaggc tgtgggcatg
      121 gagatttcac tattaaatgt ggttcgtttg caaaaggaat cgtcggattt gattgtaccg
      181 atgcatttat tcaaacagga aatgcaaatc ggagatcgaa cgttacgttc gttgtgggga
      241 acgcgaaaga tggcttaccc tttgcgtctg atgaatttga ctgtgcgtac aaccgaaaag
      301 gaccaacttc agcctatccg gaactccagc gtgtcgtgaa aaagggcgga aacattttgg
      361 gactacaccc gggtgacgat ttgggaaaag agcttccaac catttttcct catttattcc
      421 cccggtgcag tggtacgcca atagcagaca agcttacgga ccaacttgta aaaagtcggt
      481 tcacatacta tcgtattcag acgtatcatg caatcgaata tttgaaaaca ccacatgatg
      541 tggtgttgta tcgttgtttt ggacaaacac ctgaactcat atatgaagtg atggaaacat
      601 cactgccgga aattacccgc atctttaatg aacatgcaac ggtagatggt cttccaatta
      661 ccttttcaag gtacatcgta cgtgcgattg tgtaaatgaa atgatgggag agtgggacga
      721 tgatttatat tgtgaggcat ggacaaacag acgtgaatca agaggggaga ctacaaggtc
      781 gtgtaggtaa gcccttaaat tctaccggtt tcaagcaggc tgaattggtg aaagaacagc
      841 ttcaagacat tgaatttgac cttgtgttta gttctccaca ggaaagagcg agacaaacag
      901 cagcgattat caccggtagg ccggttattt ctgatcctag gattgacgta tatgacattg
      961 gagaagcgga tggaatgaag aaagacgaag tgaagagagc tggatttatt cccgatccca
     1021 ctgtttataa aggaatcgag aatccaagtg atttcgtcag tcgggttttt gatttcatga
     1081 aggagctgga gaatgaatac gggaatcagg aaatcaaaat tttaatcgct ggccaccgtt
     1141 gtacaaccgg gtgcatagga gcctattttg aagggattcc agaagaccaa aatatcctac
     1201 ggtattcctc ggataatggt gactttaagg tgtatcaatt ttctaaaact gtatagccat
     1261 acttataatc aaaataaaat ccgccagtct ccttcggttt gtccaaggga aactggcggc
     1321 tctttgttat gcttaattca atttatttaa gaagaaaaca gcaatcgtgc ctggtcctgc
     1381 atgggatcca acggccgctc cgatgagatg gagttcaact gctttcggac ggaacaattc
     1441 ttcaatcgcc gatttcattt ctaatgcagc ttctacgtca tctccatgac tgatcgcaat
     1501 tgtctgctct ccaagatgat cgccgcgttc tgccatcaat tctgttatcc ggcggaacac
     1561 ttttttgcgg ccacggtgct tctcgagtgg cacaagcttg ccatcttcca catgaagaag
     1621 cggcttaatg ttcaataggc cgccaatgaa ggcgctcgcc tttgtaactc gtccaccttt
     1681 tgctaagtag tcaaggtctt ctacggtgaa taagtgttcc atatgttcag ccatgaaacg
     1741 gattttctgt tcaattgttt taacatcatc gcccgcgtca cgaagacgga ccgcttcttt
     1801 cacgagtagt ccgtagccaa gcgaagcaca tttggaatca atgatagcaa ggtcaaagtc
     1861 cggatattct tccttaattt gatcacggat catgaccgcc gtattgtgtg tcccggatag
     1921 ttcagaggag aatgcgatgt ataatccttt ctccccactt ttcgcaagct cactccacaa
     1981 ttccataaat agctccggag atgcttggga tgttttcgga tgttgtccat tccgaatttc
     2041 cttgtatact tcctttggat tgatcgtgac aaggtcctca tattcagttt caccaatcaa
     2101 tacacggagc ggaatcaatg ttacatcatt ctcatcaaaa taagctttcg gtaaatcaca
     2161 agcactatct gcgaacagtt tcacttccat aaccccctat aaattgttcc acttatccca
     2221 agtgtaaccg ttcgcgtagt acttccgcaa tgccattttc attatttgaa agagtcactt
     2281 cgtccgcaat cgatttcagc ggtgaaatgg cattccccat tgcaacgccg actcctgcgt
     2341 actcgatcat ttcaaggtcg ttgtcctcat caccgaatgc gataatccgc tctctaggta
     2401 tatcgagcca tttcgatacg tgggaaagtc cgactgcttt attcaatcca tgccgtacaa
     2461 tttcaatgac atgccatggt gcaccccaac gacggtggtc aatcacttca gcgtgtactt
     2521 cgtggagatg ggaacggatc ggatctacct tatcctcgtc ggcttgcacg agaaggctcg
     2581 tcgggtgatc gcgtaaatat tttcggatat caccagttgt gatgttgggt ccaccgaatc
     2641 cgaatatatc gagcagtttt tcatcatgat aatgcatgta tacatcgtcc attacttcag
     2701 cgacgaggtt gatatactca aatccttcga gcgcttcaac tacatctttt acgacatgaa
     2761 ggtcaatcgt ttcatggatc gtttgccagc tcctgtccaa tggatggtgg acgaatgcgc
     2821 cgttaaaatt aacaatcggc gttgtaagtc cgagctgacg gtaatagatt tcgctcgcac
     2881 ggtatggtct tcctgtggcg atcatcactt ggtgacccgc ttctttcgct tcaagtaacg
     2941 tattttttgt ctccgatgaa ataacttttt catccgtcag tagtgtgccg tccaaatcca
     3001 gtacaatcaa atgtggcttc atcgtgattc ccccctcagg atagtaggca agtgacatca
     3061 tggctgccct tctagaagta gggcagttcc agttgccttt actttgagtg tagccaaatc
     3121 cggcttggca agtcaaaagt ttccgattct cttatcacct taattgtttt tctggaaaag
     3181 ttttgttagg ctcgtagtaa gtaaaaacgg taagggtgtg tcgcggatgt tggtattgaa
     3241 tgaaacatgg ggagcacttc ctttgctaca tgttgttcag gaagaagtag tagagcagga
     3301 agttcctgtt gtaatttttt tacatggatt tacgagtgcg aaagaacaca atcttcatta
     3361 tgcatacaat ttagcgaagc aaggaatccg ggttttattg cccgacgcgc acttacatgg
     3421 tgaaagaacg gagcatttgg atcaagtgca gcttagcctg cgattctggg aaatcgtcct
     3481 aacatcgata ggcgaagttg gggaaatata caatgaagtt aagaagcgcg ggttggcacc
     3541 agatgggaaa ataggagttg ctggcacatc aatgggcggc attgaaacac ttggctgcct
     3601 cgcagcatat cagtggattg atgttgcggg agttatgatg ggttcgcctg gatttgtcca
     3661 attggcaaaa gcacaaatgg cccaatttga aaaacggggc tttaagcttc cgattacgga
     3721 agaggaacgt caacgggtat tggaagcaat tgctgtctat gatttgacga agaagcggca
     3781 tgctttgaac aagcgtccgg ttcacttctg gcacggaatg caagacacag tcgttccgtt
     3841 cgaaccgacc cataatttct acaaagcgtt gaaggctgat tacgcagatg tccctgaacg
     3901 cctttccttt acagtggaag aggaatctgg acatgccgta tcacggagcg gaatgctgga
     3961 agcagttgat tggttggctc gtcatttgaa tcatgcatga gtggtctgct atgattgagt
     4021 aagaatgata cggaaagggg cagaacgatg gatcaagata tgaaagatag catgatgggt
     4081 gcgcttgaaa atgtcatcga cccggagctt ggtattgaca ttgtcaatct tgggcttata
     4141 tacgacgttg aactaacaga agaaggcaag tctgttgtta caatgacgtt gacatcaatg
     4201 ggttgtccaa tgggtccaat gattgttgac aatattaaaa ctgaattggc ggaacttccg
     4261 gaagtcaaag atgtcgaagt gaacatcgtc tggaatccgc cttggtcaaa agataagatg
     4321 tc
//
LOCUS       DASULI010000359         6037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_485180, whole
            genome shotgun sequence.
ACCESSION   DASULI010000359 DASULI010000000
VERSION     DASULI010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485180"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFX34_07670"
     CDS             complement(<1..262)
                     /locus_tag="VFX34_07670"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373858.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HEX5564822.1"
                     /translation="MQKNSYRILVINPGSTSTKIGVYDNDRIIFEDTLRHQADKLAQY
                     ASVIDQYDFRKDAILSSLESGGIRPSDLDAVCGRGGLLRPISG"
     gene            complement(313..1410)
                     /locus_tag="VFX34_07675"
     CDS             complement(313..1410)
                     /locus_tag="VFX34_07675"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5564823.1"
                     /translation="MEIFKYMETHDYEQLVICQDKTSGLKAIIAIHDTTLGPALGGTR
                     MWTYASEEEAIEDALRLAKGMTYKNAAAGLDLGGGKTVIIGNPKTDKNDEMFRAFGRF
                     IEGLNGRYITAEDVGTTEADMDLIHLETDYVTGVSAEFGSSGNPSPVTAFGVYKGMKA
                     AAKEAFGSDSLEGKIVAVQGVGNVAYTLCEYLHEEGAKLIVTDINKEAVQRAVDAFGA
                     TAVETDEIYSQDADIFAPCALGAIINDETIPQFKVKVIAGSANNQLKNPEHGDTIHEM
                     GIVYAPDYVINSGGVINVADELAGYNRERALKRVAGIYDTIEKIFAISKRDNIPSYVA
                     ADRLAEERIARVAKSRGQFLQNEKSVLTRKK"
     gene            complement(1442..2350)
                     /gene="yqiS"
                     /locus_tag="VFX34_07680"
     CDS             complement(1442..2350)
                     /gene="yqiS"
                     /locus_tag="VFX34_07680"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HEX5564824.1"
                     /translation="MSTLDALVERTASSGMQVVSVACAADAEVLEAVEMSIMKGIASF
                     LLFDDEERLRRTIQEYFPELEGHPYITLIHTDSKEEAAEKSVRAVSSGDAQVLMKGNL
                     PTAVILKAVLNGEYGLRTGKVLSHVAAFEVEGYDRLLFVTDAGMNIAPSLEEKAQIIR
                     NSVAAAKACGIEMPIVAPLAAIETINPVMTPTVDAASLVVMNRRGQIRDCVIDGPLAL
                     DNAVSIESARHKGITSDTAGLADILVVPSIEAGNILYKSLMYFAKAKVGGIIQGARAP
                     IVLTSRSDSAQTKLYSLALAIQTSKN"
     gene            complement(2365..4377)
                     /locus_tag="VFX34_07685"
     CDS             complement(2365..4377)
                     /locus_tag="VFX34_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5564825.1"
                     /translation="MQNVLIVGAGSGGTNILNLLLNSNFMNVSAIVDIDEQAPGLSIA
                     RNLGISHGTDWKPFLTDDVHIVFDVTGEKAVFDELLKNVKRHTLLIPGSVANLLVGLL
                     NENKQFIQKIQTEMYRQRHIFNSIDEGMIGINHEGIIDFINESACTMLEVSDFEAIGK
                     SIFEIIPDSKLVRVLKSGQPEVNEELILPSGVKIVSSRYPLISAEGETFGAFALFKDM
                     SEMIKLAEEITDLKSVKTMLEAIIHSSDDAISVVDEKGNGILVNPAYTRITGLSEDEV
                     IGKPASVDINEGESIHMKVLQTKKPVRGVNMRIGEDNRDVIVNVAPIIVDQQVKGSIG
                     VIHDITEMRSLMKELDWARQTIRKLESTCTFDDIHGSSADIGLAIDQAKIASKSDIPV
                     MLRGEAGSGKELFANAIHSGSDRKSHKFIRLNCGAIDPAVIEKEIFGENLKQDSGKTL
                     RPGLLEEAGKGTLFLDEVTDLPLSVQSRLIEYLVGKYSSNTARIIVATSKNLEKAIHE
                     GTFNMDFYYMLTRLSIQIPPLRTRKMDIPEVALHLLKKLNQEFGMNVERLSEEAMLRL
                     QNYEWPGNVRELENVLSRAMIFMKHGSAVIGEEDIRRSLFTNEVKAGEDLLPEKSTLA
                     SVMDDYERTVLEKALDEHDGNKSLTANRLGISLRSLYYKLEKYKLI"
     gene            4504..4734
                     /locus_tag="VFX34_07690"
     CDS             4504..4734
                     /locus_tag="VFX34_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HEX5564826.1"
                     /translation="MARMAAFVVLLIPILITAGGIKLMRDSLFGIQFNPFPFIWLQFT
                     VGFLMFGAGLAFFAGFLLRRDRRKGRVQDRFK"
     gene            complement(4770..5492)
                     /locus_tag="VFX34_07695"
     CDS             complement(4770..5492)
                     /locus_tag="VFX34_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5564827.1"
                     /translation="MSTKSLMSKPVVFAHRGASGVCFENTMRAFREAARQNADGIELD
                     VQLTMDGVPVVVHDTDLYRIAGIRKRVSDLHFSDLRGVKIGGKFSRRLKGYRIPTLQE
                     AVSFCELNRLALNIELKETVSDRPEFLREIIGMAMLLDSVHLSSFDPEIIKKAKAMEP
                     SIETALLVKRKTLDWGRLEKYDFIEGFNFHKRLLKEPYLGAMIATGKKLRVYGVTGNE
                     AIALHPPAYINGWITDFPGRFK"
     gene            complement(5467..>6037)
                     /locus_tag="VFX34_07700"
     CDS             complement(5467..>6037)
                     /locus_tag="VFX34_07700"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HEX5564828.1"
                     /translation="NNCRLFAKNIHVGFYILGSDVVGETVFVDKQRGKIIGGRIEALF
                     RIECGFAGNTHERTTHLYAKGIDKDVIYQEIQQLALELKELQKTARNLETQLALINGG
                     LSSQLRGEQADAYDKLQKTLQAGRDKMFEIDREIQVGLYKIKTAKPPQIEVAREAYPG
                     VIIQIGSKSSLLNKTTKGVFEVVDKILNV"
BASE COUNT         1453 a         1542 c         1245 g         1797 t
ORIGIN      
        1 cacctgaaat tggtcggagc aatcctcccc ttccacagac ggcatccaaa tccgacggcc
       61 ggatgccccc ggattccaaa gaggagagga tggcgtcctt ccgaaaatcg tattgatcga
      121 tgacagaagc atattgagca agtttgtccg cttggtgcct caatgtgtct tcgaagatga
      181 ttcgatcatt atcatacaca ccgattttcg ttgatgtaga tccgggatta ataaccagaa
      241 tccggtaact atttttttgc acgtccatcc ccctctcgat cggcaacttg tgagtaatgg
      301 tttctagatg atttattttt tgcgggtaag gacgcttttt tcattttgca agaattggcc
      361 gcgtgatttt gcaactctgg caatacgctc ttcagcaagc cggtccgctg ctacatagga
      421 tgggatattg tcccgctttg aaatcgcgaa aatcttttcg atcgtatcat atattccagc
      481 gacccgtttc aatgcacgtt cccggttata gcctgccagt tcatctgcta cattgatgac
      541 gccgccggag ttgatgacgt aatcgggtgc atagacgatt cccatttcat ggatcgtatc
      601 gccgtgctcc ggatttttca attggttatt cgcagaccct gcgatcactt tcactttgaa
      661 ttgagggatc gtttcatcgt tgatgatggc ccctaaggca caaggtgcga aaatatctgc
      721 gtcttgtgaa tagatttcat ccgtctcgac agctgttgca ccaaatgcat caactgcacg
      781 ttggacggct tctttattaa tatccgtgac aatcagcttc gcgccttctt catgcaagta
      841 ttcacaaagt gtgtaggcga cgttgcctac gccttgtaca gcaacgatct tgccttcaag
      901 ggaatcactt ccgaatgctt ctttagccgc agccttcatg cctttgtaaa ctccgaatgc
      961 cgttactggc gaagggttgc ccgaagaacc gaactctgcg gatacgccag ttacataatc
     1021 cgtttccaaa tggatcaagt ccatatccgc ttctgtcgtg cccacgtctt cagctgtaat
     1081 atatcttccg ttcaatcctt cgatgaatcg gccgaatgca cggaacatct cgtcattttt
     1141 atccgttttc gggttcccga tgatgaccgt cttgccgccg ccaaggtcaa gccctgccgc
     1201 cgcattttta taagtcatgc ctttcgccag acggagtgca tcctcgattg cttcctcttc
     1261 actagcatat gtccacatgc gcgtgccgcc tagtgcaggt ccaagtgtcg tatcgtggat
     1321 tgcgatgatc gccttcaacc ctgaggtttt atcttggcag atgaccaatt gttcataatc
     1381 gtgagtttcc atatatttaa aaatttccat gatgtgtttc ctcctccgga aaagtccgtt
     1441 tttagttttt ggatgtttga atggctaacg ccaaggaata aagcttagtt tgtgcgctat
     1501 cagatctcga ggtcaatacg atcggcgcac gtgcgccttg aatgatcccg ccgacctttg
     1561 ctttcgcgaa atacatgagc gatttatata agatatttcc tgcctcgatt gaaggcacta
     1621 cgaggatgtc agcaagtcct gcagtatcgc tcgttatccc tttatgcctg gcggattcga
     1681 tcgatacagc attatccagc gcaagggggc cgtcgatgac gcaatccctt atttgtcctc
     1741 tccggttcat gacaacgagc gatgcagcat caactgtcgg cgtcatcacc gggtttatcg
     1801 tctcgatcgc tgcaagcggg gcgacaatcg gcatttcgat gccgcacgct ttggccgctg
     1861 caaccgaatt ccgtatgatt tgtgccttct cttccaggct aggtgcaatg ttcatgcctg
     1921 catccgtcac aaaaagcaac cgatcatagc cttccacttc gaaagccgcc acatgagaaa
     1981 ggacttttcc agtccgtaac ccgtactcac cgttcaatac agctttcagg atgactgcgg
     2041 tcggcaagtt gcctttcatc aggacttgtg catccccgct tgatacggcg cgcacggatt
     2101 tttccgccgc ctcttccttg ctatccgtat gaatgagtgt gatatatgga tgcccttcca
     2161 gttcagggaa atattcttgt atcgtccgac gcaatctttc ttcgtcatcg aatagaagaa
     2221 aagatgcaat tcctttcata atcgacattt ccactgcctc taacacttca gcatccgcag
     2281 cgcatgcaac ggatacaact tgcataccgg aagaagccgt tctttcgact aaagcatcca
     2341 gtgtcgacat ttctgcagcc tccttcagat gagtttatat ttctccaatt tgtaatataa
     2401 ggagcgcaat gaaatgccta gacggttcgc tgtcaatgat ttattaccat catgttcatc
     2461 caacgccttt tcgagcacgg tccgttcgta atcgtccatc acagaagcca aggtgctttt
     2521 ctcagggagg agatcttccc cggctttcac ttcatttgtg aaaagggacc ttcttatatc
     2581 ttcttcccct atgacagcag atccatgctt cataaaaatc attgcacggc ttagaacatt
     2641 ttcaagttcc cggacattgc ccggccattc atagttttga agcctgagca tggcttcttc
     2701 cgataatctt tccacattca tgccaaactc ctggttcaac tttttcaaga gatgaagtgc
     2761 aacttccggg atatccatct tcctcgtacg caaaggtgga atctggatgg acaaccgagt
     2821 caacatataa tagaagtcca tgttgaaagt gccttcatgg attgcctttt ccaggttctt
     2881 ggatgtagcc acgataatcc ttgccgtatt agaagagtac ttgccaacca aatactcgat
     2941 gagtctagat tggaccgata atggcaaatc agtcacttcg tctaaaaaga gggttccttt
     3001 tccggcttcc tcaagcaagc cgggccttag agtttttccg gaatcctgtt ttaggttttc
     3061 cccgaagatt tccttttcga ttacagcggg gtctatcgct ccgcagttca ggcggataaa
     3121 cttatgggac tttctgtcac taccgctatg aatggcattt gcgaataatt ctttccctga
     3181 tcccgcttcc ccccgcaaca tgaccgggat atccgatttc gaggcgattt tcgcctgatc
     3241 aatcgcaaga ccgatatccg cggagcttcc atgaatatca tcgaatgtac atgtcgactc
     3301 caatttcctt attgtttgtc ttgcccaatc cagttctttc ataagactgc gcatttcggt
     3361 gatgtcatga atgacaccta tacttccctt cacttgctga tcgacaatta tcggggctac
     3421 attcacgatg acatcccgat tatcttcccc gatccgcata ttgacgcctc ggacaggttt
     3481 ttttgtctgc aacactttca tatggatgct ttcgccttca ttgatgtcga ccgatgcagg
     3541 ttttccaatt acctcatctt cagataagcc tgtgatccgc gtatatgccg gattgacaag
     3601 aatgccattc cctttttcat cgacaactga aattgcgtca tcacttgaat ggataattgc
     3661 ttctagcatc gttttcactg atttcaaatc ggtgatctct tcagcgagtt tgatcatctc
     3721 agacatatcc ttgaataacg caaacgcgcc aaatgtttcc ccttccgccg atataagcgg
     3781 atagcgggaa ctgacgattt taaccccact cggcaggatc aattcttcat tcacttcagg
     3841 ctgaccagac ttcagcaccc tgaccaactt gctgtcaggt atgatttcaa aaatcgattt
     3901 gccaatcgct tcgaaatcag aaacttccaa catcgtgcaa gcgctttcat taatgaaatc
     3961 gattatgccc tcatgattga ttccgatcat gccctcatca atcgaattga aaatgtgccg
     4021 ctgccggtac atttctgttt gaatcttttg gatgaactgc ttattctcat tcaggaggcc
     4081 gacaagaaga ttcgcgactg agccgggaat caaaagcgta tgcctcttga cgtttttcag
     4141 caattcatca aaaaccgctt tttcacctgt tacatcaaat acaatatgga catcatctgt
     4201 caggaacggc ttccaatcgg tgccgtgcga gattccgaga ttcctggcaa tggatagtcc
     4261 tggtgcttgt tcatcaatgt cgacaattgc cgacacattc atgaaattcg agttcaagag
     4321 aaggttcagt atgttcgtgc caccagaccc agctccaaca atgagtacat tttgcaagga
     4381 tattcattct ccttttagca ccatgcaatc ttttgcaaat ctatattcat catacacgat
     4441 tgcactggtc ttgacaatat tggttgggca ggtaaaatta aaattggtaa ggagactgat
     4501 attatggcac gtatggcggc atttgtcgtt cttttgattc ccattcttat cactgcaggc
     4561 ggcattaagc taatgcgtga ttcacttttc ggaatccagt tcaatccgtt ccctttcatc
     4621 tggcttcaat tcacggtcgg ctttctcatg ttcggtgcgg gtctcgcatt ctttgcaggt
     4681 tttctcctgc gccgtgatcg ccggaaggga cgggttcaag atcggtttaa ataataaaca
     4741 aaaaagcgca ggacaccctg cgcttttttc tatttaaacc tccctgggaa atccgttatc
     4801 cagccgttga tatacgcagg cggatggaga gcaattgctt cgttgcccgt taccccatag
     4861 acccgcagct ttttaccggt ggcgatcatt gcgccaagat aaggttcctt cagcaatcgt
     4921 ttatggaagt tgaagccttc tatgaaatcg tatttttcca aacgtcccca atccaacgtt
     4981 ttccttttga caagcagtgc cgtttcgata gacggttcca tcgcttttgc ctttttaata
     5041 atttccggat cgaatgatga caggtggacg ctatccaaaa gcatggccat accaataatt
     5101 tcacgcaaaa attccggcct atcggatact gtctctttca actcgatatt caaagccagt
     5161 cgattcaatt cacagaatga aacagcttcc tgcaatgttg gtatgcgata ccctttaagc
     5221 cgcctggaaa acttaccgcc aattttcacg cctcgcaaat cactgaaatg taggtcggaa
     5281 acgcgtttcc gaatgccggc tattctataa agatccgtgt catgcaccac gacggggact
     5341 ccgtccatcg tcaattgaac atccaattca attccatcgg cattttgcct tgcggcttcc
     5401 ctgaaggcac gcattgtgtt ttcaaaacag acaccggatg cacccctatg tgcgaataca
     5461 acgggtttag acattaagga ttttgtcgac aacttcaaag acacctttcg tcgttttatt
     5521 cagcaagctg gattttgagc cgatttgaat gatgacacct ggataggcct ccctcgcaac
     5581 ttcaatttgg ggcggtttgg ccgttttaat tttgtaaagt ccaacttgta tctcccggtc
     5641 gatttcaaac atcttgtctc gtccagcctg aagcgtcttt tgtagtttat catatgcatc
     5701 agcctgctct ccgcgcaact gacttgaaag tccgccatta attaaagcca attgcgtttc
     5761 aagatttctg gctgtcttct gcagttcctt caattccaaa gccaattgtt gtatttcctg
     5821 atagatcaca tccttatcaa tccccttcgc atacaaatgg gttgtccttt catgtgtgtt
     5881 gccggcgaat ccgcattcaa tcctgaagag tgcttcaata cggccgccaa tgattttccc
     5941 tctttgttta tcaacaaaaa ccgtttcacc gactacatca gagcccaata tatagaatcc
     6001 gacatggatg ttttttgcaa agagcctgca attgttt
//
LOCUS       DASULI010000360         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_237084, whole
            genome shotgun sequence.
ACCESSION   DASULI010000360 DASULI010000000
VERSION     DASULI010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237084"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VFX34_07705"
     CDS             <1..581
                     /locus_tag="VFX34_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX5564829.1"
                     /translation="AACESVGADGLLARVGSYYHDIGKTKRPGFFIENQHGAVNPHDE
                     LPPEKSRDIIIAHANDGADLLAKHKMPIEIIDIARQHHGTSFLKFFFVKAQEMGQEVV
                     EDDFRYPGPKPQTKEIAIISIADSVEAAVRSLKEPTPDRIAKLVRSIITSKLNDGQFD
                     ECDLSMKELKTIENVICETLNGIFHSRIEYPD"
     gene            601..1071
                     /gene="ybeY"
                     /locus_tag="VFX34_07710"
     CDS             601..1071
                     /gene="ybeY"
                     /locus_tag="VFX34_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5564830.1"
                     /translation="MLDIYFNDETGKVTEDITQLVEDVLNHAAGMEGLSDHPEVSVTF
                     MTDEEIRKINAEYRGKDKATDVISFALEEMTEGEVAIVPEEGMPIVLGDILISIETAA
                     RQSSEYGHDEKREIGFLALHGFLHLLGYDHMTEEDEKKMFGRQKEILTSFGLER"
     gene            1078..1425
                     /locus_tag="VFX34_07715"
     CDS             1078..1425
                     /locus_tag="VFX34_07715"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX5564831.1"
                     /translation="MHRFFKSFQYAFKGIRHGLLTERNFRFHALAIVIVSIAGVLTGL
                     NTTEWSIIFILFGGMTALELINTAIERTVDLVTKENHPLAGQAKDLASGAVLIFAIFS
                     AIIGMIIFIPKWF"
     gene            1449..1862
                     /locus_tag="VFX34_07720"
     CDS             1449..1862
                     /locus_tag="VFX34_07720"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008431620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX5564832.1"
                     /translation="MDKVKLMEEAKIARERAYVPYSKFKVGAALLTADGQIIHGCNIE
                     NSGYSMTNCAERTAFFKAISEGIMDFKALAVTGDTEGPISPCGACRQVIAEFCDSNMP
                     VYLTNLKGDVTETTVGELLPGAFSKEDLDYAEGQQ"
     gene            1843..2757
                     /gene="era"
                     /locus_tag="VFX34_07725"
     CDS             1843..2757
                     /gene="era"
                     /locus_tag="VFX34_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5564833.1"
                     /translation="MQKDSNNFKSGFISIIGRPNVGKSTFLNRVVGQKIAIMSDKPQT
                     TRNKVQGVVTTDDSQLVFIDTPGIHKPKHKLGDFMVKSARNTLKEVDVIMFMVNANEP
                     IGGGDKFIIDLLQNTETPVFLVINKIDLVHPDDLLTIITSYTKEYDFAEIVPLSALNG
                     NNVERLMETLTNYLPEGPKYYPDDQVTDHPERFIISEFIREKVLHLTREEIPHSVAVV
                     IEKIERDESRGLVDVAATIIVDRDSQKGIVIGKKGALLKEIGTKARHDIEMLLGSKVY
                     LELWVKVQKDWRNKPGQLREFGFREDEY"
     gene            2773..>3472
                     /gene="recO"
                     /locus_tag="VFX34_07730"
     CDS             2773..>3472
                     /gene="recO"
                     /locus_tag="VFX34_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5564834.1"
                     /translation="MLNKWEGIILRSIPYGESNKIVTLYTREAGKMTAMARGAKKPAS
                     RLAAVTQTFTHGFFLLRSGRGMGTLEQGEPIDSMRHIREDLEATAYASYIVELIDKLT
                     EDHGQGPNVFGLLHEALHAINEQYDPEAIALFVEWKMLPVAGVHPVLHQCANCGATEG
                     EFAFSFQEIGFLCHRCFQIDPYLVRITPTQLRLIRTFFTVPIQQVGSLNLKKPTKQFM
                     KKIVRTIYDEQVGVR"
BASE COUNT         1036 a          702 c          887 g          847 t
ORIGIN      
        1 atgcagcatg tgaatccgta ggggcggatg gactgttggc acgggtagga agctattatc
       61 atgatatcgg gaagacgaaa agaccgggtt ttttcattga aaatcaacat ggcgcagtga
      121 atccgcatga tgaattgcca ccggaaaaaa gccgtgatat cattattgcc catgcaaatg
      181 atggggcaga cctgcttgcc aagcataaaa tgcccattga aatcatcgat attgcaagac
      241 agcatcatgg aacaagcttt ttgaagtttt tctttgtaaa ggcgcaggaa atgggacagg
      301 aggtagttga ggatgatttc cgctatccgg gaccgaaacc gcagacgaag gaaattgcaa
      361 tcatttcaat tgcggacagt gttgaagctg cagtacgttc attgaaggaa ccgactccgg
      421 atagaattgc gaaattggta aggtcaatca tcacctcgaa attgaatgat gggcaattcg
      481 atgaatgcga tttgtcgatg aaagaactga aaacgatcga aaatgtcatt tgcgaaacat
      541 tgaacggcat tttccatagc cggatcgaat atccggactg atcagaagga aggcgaaaga
      601 atgctcgata tttactttaa tgacgaaaca ggaaaagtga ccgaagacat tacacaactt
      661 gtagaagacg tcttgaatca tgctgccgga atggaagggc tctccgatca tcctgaagtt
      721 tccgtcactt tcatgacgga cgaggaaatc aggaaaataa acgccgaata tagagggaaa
      781 gacaaggcga ccgatgtcat ttcatttgcg cttgaggaaa tgacggaagg ggaagtcgcc
      841 atagtcccgg aagagggaat gccaatcgtc ctcggtgata ttcttatttc catcgagacg
      901 gcggcgcggc aatcatctga atatggccat gacgaaaaac gggaaatcgg attcctggcg
      961 ttgcacggtt tccttcatct tcttggatat gatcatatga cagaggaaga tgaaaagaag
     1021 atgttcggaa ggcaaaagga aatcttgaca tctttcggtc tggaaaggta agcgaaaatg
     1081 catcgtttct tcaaatcttt tcaatatgca tttaagggga tcaggcatgg cttgctgacg
     1141 gaaaggaatt tcaggttcca tgcccttgcc atcgtaattg tatcgattgc gggcgtttta
     1201 acaggtctca atacgactga atggagtatc atcttcattc tatttggcgg aatgaccgct
     1261 ttggagttga tcaatacagc gattgaacgg acggtcgatc tcgtcacaaa agagaatcat
     1321 cctcttgctg ggcaggcgaa ggaccttgca tctggagccg ttttgatttt cgctatcttc
     1381 tccgcaatta tcggcatgat catcttcatt cctaaatggt tctaataaac ttacgtagag
     1441 gtgtaattat ggataaagtg aaattgatgg aagaagcaaa aatagcacgt gaaagggcat
     1501 atgtacccta ttcaaaattc aaagtcgggg ctgcactgct gacggcggat ggacaaatca
     1561 tacatgggtg caatattgag aattcaggct atagcatgac gaattgcgca gaaaggacag
     1621 ctttcttcaa agccatctca gaggggatca tggacttcaa agcattggcg gtaaccggcg
     1681 atacggaagg cccgatttca ccatgtggcg catgtagaca agtcattgca gaattctgtg
     1741 actctaacat gcctgtctat ttgacgaatc tgaaaggcga tgtgacagag acgacagttg
     1801 gggaattgtt gccgggggcg ttctctaagg aggatcttga ttatgcagaa ggacagcaat
     1861 aatttcaaat cgggcttcat atcaatcatc ggccggccga atgtcggcaa atccacattt
     1921 ttgaatcggg tcgtcggtca gaaaatcgcc atcatgagcg ataaaccgca aacgacgagg
     1981 aacaaagtgc aaggcgttgt cacgacagac gactcccagc ttgttttcat cgacacgccc
     2041 ggcattcata agcccaagca taagcttggg gatttcatgg tgaagtccgc acgcaatacg
     2101 ctaaaggaag ttgatgtcat catgttcatg gtgaatgcca atgaaccgat tggcggcggg
     2161 gataaattca tcatcgattt attgcaaaac acggagacgc cagtcttcct tgtcatcaat
     2221 aagattgacc ttgttcatcc ggacgaccta ttgacgataa tcacttccta tacgaaggag
     2281 tatgattttg cagaaatcgt tccattgtcc gcattgaacg gcaacaatgt cgaaagatta
     2341 atggaaacgt tgacgaacta tttgccagaa ggcccgaagt attatccgga tgaccaagta
     2401 accgaccatc cggagcgatt catcatttcc gaattcatcc gcgaaaaggt tttgcatttg
     2461 acccgggaag aaatcccgca ttccgtcgct gttgtcatcg agaagatcga aagggatgaa
     2521 agccgcggcc tcgtagatgt ggcggctaca atcattgtcg atcgggactc tcaaaaagga
     2581 attgtcatcg gtaaaaaagg tgccctttta aaggagatcg gtacaaaggc aaggcatgac
     2641 atcgaaatgc tattaggatc aaaagtgtat ctggagctat gggtgaaggt gcagaaggat
     2701 tggcgcaata aaccaggaca actccgggaa ttcggattcc gtgaagacga atattgatta
     2761 ggagtggtat ccttgctgaa taaatgggaa gggatcatcc taagaagcat tccgtacggc
     2821 gaatcgaaca agattgttac gctttataca cgcgaggcag gtaaaatgac tgcgatggca
     2881 aggggagcga aaaaaccggc cagccgatta gccgcagtga cacagacgtt cacacatgga
     2941 ttttttcttt taagatccgg acgaggaatg ggtacgcttg aacaaggcga gccaattgat
     3001 tcgatgaggc atatccgcga ggaccttgaa gcgacggcat atgcgagtta catagtggag
     3061 ctgatcgaca aattaactga agaccatgga caaggcccga atgtctttgg attgcttcac
     3121 gaagcgttgc atgccatcaa tgaacaatac gacccggaag cgatcgcatt atttgtcgag
     3181 tggaagatgc ttcctgtcgc gggtgtccat cctgtccttc atcaatgcgc aaactgtgga
     3241 gcgactgaag gggaattcgc attttcgttt caggaaatcg gattcctttg ccatcgttgt
     3301 ttccagatag acccgtatct tgtaagaatt acaccgaccc aattgaggct gatccgcact
     3361 tttttcacag ttccaatcca acaggtcggt tcattgaatt tgaaaaagcc gacaaaacaa
     3421 ttcatgaaga agatagtgag aactatttat gatgaacaag tgggggtccg at
//
LOCUS       DASULI010000361         6952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_224473, whole
            genome shotgun sequence.
ACCESSION   DASULI010000361 DASULI010000000
VERSION     DASULI010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224473"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFX34_07735"
     CDS             complement(<1..602)
                     /locus_tag="VFX34_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564835.1"
                     /translation="MSYLRVKNLKYKYPESSGFALDDISFAVEKGEFIGIVGMNTAGK
                     TTLCYALSGLVPHFFKGAYGGDVFIGDMDVLSHDISEITASVGLVFENPFSQMTGAKF
                     TVYDEIAFGLENQGVPRDVMHERVRESMKLLDIEGLRTQNPFSLSGGQMQRVAIASVI
                     AMKPDILILDEPTSQLDPEGAEEVFRVVEKLADGGMTIIMV"
     gene            complement(599..1366)
                     /locus_tag="VFX34_07740"
     CDS             complement(599..1366)
                     /locus_tag="VFX34_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX5564836.1"
                     /translation="MKNMTLYVGRDSPVHRVDPLTKLLFVLISITSTYILSNHLIVFG
                     ILLFTFFILLIGKVFNSILPVIGVSFLLIASIIIVQGFFHPDRATVLFEIGPIAIYKE
                     GFLIALLITLRVVNMVAAFGVLILTTKPDDLVEALLRKGMSPKFGYVLLSVLQIIPQM
                     VALTGKITDAQKARGMETEGGLWMRFKSFLPLLGPVVLNSLTETRERSIALEIRGFNA
                     KGKRTFLYERKNYRYSLPIRVALIAFFLAVIVWRVMS"
     gene            complement(1391..1993)
                     /locus_tag="VFX34_07745"
     CDS             complement(1391..1993)
                     /locus_tag="VFX34_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX5564837.1"
                     /translation="MKKKRLWTLQFSTAAFVLIPAAVGINFLGKLFADVLKLPLWIDA
                     IGTVLASMLAGPVVGAIAGVVNNIVYGFTTSPVSFVYAITQAAIGLTVGIMAYKGWIS
                     NVGKAIVLGIVVGFIAAIVSTPLNIMFWEGTTGNVWGDALFATMLAKDMPLWLASFAD
                     SIVVDVPDKIVTVLLSFLIFKGLPKNVKLMYDNQGEIERL"
     gene            complement(2218..2565)
                     /locus_tag="VFX34_07750"
     CDS             complement(2218..2565)
                     /locus_tag="VFX34_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX5564838.1"
                     /translation="MKLEEMTMEQIWTEIDAQLGANKGPIEGMNASYSFDLSGEDGGM
                     YGLKFADGEAETFRGDPGEVDCTLKMSVGDFKKLLGGNLNSTAAFMMGKLKVKGNIGL
                     ALKLENLLKQYTF"
     gene            2731..4626
                     /locus_tag="VFX34_07755"
     CDS             2731..4626
                     /locus_tag="VFX34_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HEX5564839.1"
                     /translation="MNRSQDELNMILEELQKKQEFIFMQAKQSDLSRQSIAPVLEELC
                     IRIAKIMACDRVSIWLFNESQTSLTAQNIFHLNKSMHTFGEVILAEELPSYFEAVQQE
                     RILAIEDVSTDPATQELTRLPSYNDGEVKSLIDASIILSKGIGGVVCCESIQPKTWTY
                     LDRVLAASIADMLSFIFDRLERIEVEEHVHQLAYTDLLTGLDNQYSFSEKVTDLIPML
                     EEGHRGLFVYLVMDQFTDMQSVLGPDGADQLLQITANRLKTLFPYPAQTTRLAFDHFV
                     IFTPFEGELECEMTRVERLINRLKRPMHISGQEVYMTFSYGVSIYPEHATSVKYGVQA
                     AYMALQSGKETANRKAWNLYNPDMHTFLKETMLSEMNLRRGLDMNEFQLHYQPQVNCR
                     TGEVSGFEALIRWQHPERGLIFPGEFIELAESTGLITPIGEWVIKQAASQIREWNEQG
                     LKNATVSVNISPRHFLHHNLPFYLDKCVKEANIKPKSLVLEITENVAMEDTEAVANRI
                     NLFREMGYSVSIDDFGKGYSAFIYLQHFPIQQIKIDRQFINGLGKNPKSSGIVQTIIH
                     LAEMLGLQTIGEGVETKQQWDLLKQFGCSELQGFYFSKPIPVDEINDLIRKYGRGGKL
                     LLPSTFQ"
     gene            complement(4835..6487)
                     /locus_tag="VFX34_07760"
     CDS             complement(4835..6487)
                     /locus_tag="VFX34_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="b(o/a)3-type cytochrome-c oxidase subunit 1"
                     /protein_id="HEX5564840.1"
                     /translation="MLKNVLFPKKEARLYMSFMHVTFASLLIGGLMGLLQTLSRSGQF
                     QLPFGINYYTILTVHGVILGLVLTTFFIVGFQFALMGKTVGMSDKQRQWAWIAFWTML
                     VGTIIAAITILVGQANVLYTFYAPLRAHAAFYIGLALVIVGSWIAVFVNFRQLYVWKK
                     ANPGQKSPLLAFMVVINMAMWFIASLGVASSVLIQFIPWSLGYAATINVLLSRTLFWY
                     FGHPLVYFWLLPAYMAWYAIIPKIIGGKLFSDSLARLSFILLLMFSIPVGFHHQLTEP
                     GIDPTWKFIQVVLTFMVVIPTLMTAFSIFATFETTGRRKGHKGLFGWFKHLPWKDVRF
                     LAPFIGMVAFIPGGAGGIVNASHQVNTLIHNTIWVTGHFHLTVATTVILTYFGIAYWL
                     VPHLTGRRLTPKLNKLGIIQTIIWTVGMTIMSTAMHIQGLLGGPRRSNFSNYAGGAQV
                     DTWISYQIAQAVGGTILFIGIILMIYIFIQLAFFAPRGVQEFPIAEEEVDAEPTPKIL
                     ENWYLWIGITVALILFAYTIPVIDIIKHSPPGSLPFDWPIGR"
     gene            complement(6487..>6952)
                     /locus_tag="VFX34_07765"
     CDS             complement(6487..>6952)
                     /locus_tag="VFX34_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5564841.1"
                     /translation="GLGSLALFLIIIGFAAFWKGTHPMSHIETIDPQNVEAHESFKPE
                     NLGLREVAEGKYTVNIVASAFNYDFGKDADGKPVKKIRVPKGSTVLFQITTKDVVHGF
                     QVAGTNVNMMVEPGHVSRLETVMKNAGEFTVVCNEYCGIGHHQMFATVEVFE"
BASE COUNT         1918 a         1689 c         1653 g         1692 t
ORIGIN      
        1 accataatga tcgtcatgcc gccgtccgcc aacttttcaa cgacgcggaa cacttcctcc
       61 gcgccttccg gatcgagctg ggaagtcggt tcgtcgagga tgaggatgtc aggcttcatg
      121 gcgatgacgc tcgcaatggc gacccgctgc atttggcctc cggataatga aaaggggttc
      181 tgggttcgca gcccttcaat atcaagcagc ttcatacttt cacggacccg ctcatgcatg
      241 acatcccgcg gcaccccttg gttttccaag ccgaatgcga tttcatcata gacggtgaac
      301 ttggcgccgg tcatttgcga gaaaggattt tcgaaaacaa gtccgacgct tgctgtgatt
      361 tcactgatat catgcgacaa cacatccatg tcgccgatga atacatcgcc gccatacgcg
      421 cctttgaaaa agtgggggac gagtccgctc agtgcgtagc acaacgtcgt ctttcctgcg
      481 gtattcatgc cgacaatccc gatgaattcg cctttttcca ctgcgaatga aatgtcatcg
      541 agcgcaaaac cggagctttc cggatactta tatttcaagt tcttcacacg caagtaactc
      601 atgacatcac cctccaaacg atgactgcca aaaagaaggc gatcaatgca actctgatag
      661 gcaagctata tcgatagttt ttccgttcat acaaaaatgt ccgtttccct ttcgcattga
      721 atccgcggat ttcgagggcg atggaacgct cccgtgtttc cgtaagggaa ttcaggacaa
      781 ccggaccaag tagggggagg aatgatttga acctcatcca caatccgcct tccgtctcca
      841 tgccacgagc cttctgcgca tctgtgattt tgccggtcaa ggcaaccatt tgcggaatga
      901 tctgcaatac agacagcaac acataaccga atttaggcga catgcctttc cgcagcaatg
      961 cctccaccag atcatccggc tttgtcgtca aaatgagcac cccgaaagct gccaccatat
     1021 tgacgacccg gagcgttatc agaagtgcta tcaaaaagcc ttctttgtaa atggcaatcg
     1081 ggccgatttc gaacaggacc gtcgcccggt cgggatggaa aaatccttgg acaataataa
     1141 tggaagctat caatagaaaa ctgaccccga tgaccggcaa aatggaattg aacactttgc
     1201 cgatcagcag gatgaagaat gtgaacagaa ggattccgaa cacgattaga tgattcgaca
     1261 ggatatatgt cgatgtgatg gaaatgagta cgaataatag ctttgtcaat ggatccaccc
     1321 gatggacagg tgaatccctg ccgacgtaca atgtcatgtt cttcattcgg atccctcccg
     1381 caggcaatgc ttacaatctt tctatttctc cttgattgtc atacatgagc ttcacatttt
     1441 tcggcagccc tttgaagatc aagaagctga gcagcacagt gacgattttg tcgggtacat
     1501 cgacgacaat gctgtccgcg aaagatgcga gccataacgg catgtctttt gcgagcatcg
     1561 ttgcaaacaa ggcatctccc caaacgtttc ctgtcgtgcc ttcccaaaac atgatattga
     1621 gcggcgtcga tacaatcgct gcaataaatc cgacgacaat cccgaggacg atcgctttgc
     1681 cgacattcga aatccagcct ttatacgcca taattccaac cgttaaaccg atggcagcct
     1741 gggtgatggc atagacgaag gaaactggac tcgtcgtgaa gccgtagacg atattattga
     1801 caacaccggc aattgcacct acgacgggcc ctgcaagcat gctcgccaaa acagttccga
     1861 tcgcatcgat ccataggggc agctttaaca cgtccgcaaa cagctttcct aaaaagttga
     1921 tgccgaccgc tgccggtatc aaaacaaatg cagccgttga aaactgcaat gtccaaagac
     1981 gtttcttttt catttgtatt ctcctttttc ttagtaatca agatggttgt caagacacat
     2041 gaatatacta attcattttg aagaaatagg gaagtctttc ttgaaaaaga gatgacgact
     2101 tattctcatc ttatgcccga gagcattttt aggtattcaa aagaccaaga gaatacaagt
     2161 tcatgtgtcc cttggtctag atgtttagtg agctagaact ggcagtttta ttgagaatca
     2221 aaaggtatac tgcttcaata aattttcaag cttcagcgcg aggccgatat tgcctttcac
     2281 tttcaatttc cccatcatga aagcagcggt ggaattcaaa ttgccgccaa gcaatttttt
     2341 gaaatcgcca acgctcatct tcaaggtgca atcgacttca cccgggtcgc cgcggaacgt
     2401 ctccgcctct ccgtcggcaa actttaatcc atacatgccc ccgtcctcac cggataagtc
     2461 gaaagaatac gaagcgttca tcccttcaat cggaccttta ttggccccca gctgcgcatc
     2521 aatttcagtc caaatctgtt ccattgtcat ctcttccaat ttcataagga gacaccctct
     2581 tcgtaagtaa tgagtgatta ttcattcatt atagcatact ggcgcaaaaa ggaattatga
     2641 aaatataaga tttatcctca ataaatctag aattttgtcg atagaaaggg gtagtacata
     2701 tagtcagcag aaaggggttg tagaagtccg atgaatcgtt cccaggatga actcaatatg
     2761 atcttggaag aacttcagaa aaaacaggaa ttcatattta tgcaagcgaa gcagtcggac
     2821 ctttcgcgac aatcgattgc ccctgtatta gaagaattgt gcatccgtat cgcgaaaata
     2881 atggcgtgcg atcgagtgag catttggtta ttcaatgaaa gccaaacaag cttgactgca
     2941 caaaatattt ttcatttaaa taaatccatg catacattcg gggaagttat ccttgccgaa
     3001 gagctgccgt cttattttga agcggtccag caagaacgaa ttttagcgat tgaagacgtt
     3061 tcaacagatc ccgccactca ggaattgacg agacttcctt cctacaatga tggggaagtg
     3121 aagtcgctta tcgacgcttc gatcatctta agcaagggaa tcggaggtgt tgtctgctgc
     3181 gaatcgatcc agccgaaaac gtggacatac cttgataggg tcctggcagc gtccattgcg
     3241 gacatgttat ccttcatttt tgaccggttg gagcgcattg aagtggaaga acacgtccat
     3301 cagctcgcct ataccgatct tttgaccggg ctcgacaacc aatattcgtt ctctgaaaag
     3361 gtgacggatc tgattcctat gttagaagaa ggacatcgtg gattgttcgt ttaccttgtg
     3421 atggatcagt ttacggatat gcaaagtgtg ctaggacctg acggggcgga ccaattgctg
     3481 cagattacag cgaaccgtct gaagacgcta ttcccgtatc ctgcacaaac cacaagactg
     3541 gcattcgacc atttcgtcat ttttacgcca tttgaaggcg aactggaatg cgaaatgacg
     3601 agggtggagc ggctcatcaa ccgattgaaa cgtccgatgc atataagcgg acaggaagtg
     3661 tatatgacat tcagctacgg ggtatccatt tatcccgagc atgcgacgag cgtgaagtac
     3721 ggcgttcagg cagcgtatat ggcgctgcaa tccggaaaag agacagcaaa caggaaagct
     3781 tggaacctct ataatccgga catgcatact tttttgaaag agacaatgct ttcggagatg
     3841 aacttgcgaa gaggccttga tatgaacgaa tttcagttgc attatcagcc acaagtgaat
     3901 tgccggacgg gagaagtgag cggcttcgaa gcgctcatcc ggtggcagca tccggagcgc
     3961 ggacttattt ttcctgggga attcatcgag cttgccgagt cgacgggact catcacaccg
     4021 atcggggagt gggtcatcaa gcaggcggcc agccaaatcc gggagtggaa tgaacaaggg
     4081 ttgaagaatg ccacggtatc ggtgaatata tcaccaaggc atttcctgca tcataatctt
     4141 ccattctatt tggataaatg cgttaaggaa gcgaacatca aaccgaaaag ccttgtactg
     4201 gaaataacgg aaaatgtcgc tatggaagat acggaagccg tcgccaaccg gattaatcta
     4261 tttcgagaaa tgggttattc cgtttctatc gacgattttg gaaaagggta ttctgcattc
     4321 atttatttgc agcattttcc gatccaacag atcaagatcg accgtcaatt catcaatgga
     4381 ctcggaaaga atccgaaaag ctccggaatt gtccagacga tcattcatct tgccgaaatg
     4441 cttggactgc aaacgatcgg tgaaggtgtc gaaacgaaac agcaatggga tcttttgaaa
     4501 cagttcggct gctcagagct gcaagggttt tatttcagca aaccgatccc tgtcgatgaa
     4561 atcaatgacc tgatccgcaa atatggaaga ggcggaaagc tgcttctacc atctacattt
     4621 caatagagaa aagccttacg cgtcggggaa tccccattgc gtaaggcttt attaatgtta
     4681 gcatctagac ttcgggcgcc agagactcgg gatcataagt caaagctgct ctgtggcaaa
     4741 ataccattgc tcccaacgct tcgcttttgc tcgcaaaagc cgttctacgt gacggctttg
     4801 cctgtcgcct ctaggcaggc gccctcctct tttgttatcg accaatcggc cagtcaaatg
     4861 gcaacgaccc tggcggggag tgcttgataa tgtcgatgac cggaattgta taggcgaaga
     4921 ggatgagcgc aaccgtaatt ccgatccata aataccagtt ctccaggatt ttcggcgtcg
     4981 gctctgcatc gacctcttcc tctgcaatcg gaaattcctg tacgccgcgc ggggcgaaga
     5041 acgcgagctg gatgaagata taaatcatca agatgatgcc gatgaaaagg atcgtgcctc
     5101 cgacagcttg agcgatctga tagctgatcc atgtatcgac ttgcgcgccg ccagcataat
     5161 tcgagaagtt ggagcgtctc ggacccccta gaagtccttg gatatgcatc gctgtcgaca
     5221 tgatcgtcat cccgacagtc cagatgatcg tctggatgat gccgagctta ttcaacttcg
     5281 gcgtcaaacg tcttcctgtc aagtgaggga cgagccaata agcgataccg aaatatgtca
     5341 ggatgactgt tgtcgcaact gtcaaatgga agtggcccgt cacccagatc gtattatgga
     5401 tgagtgtatt cacttggtgg gaagcattga cgataccgcc ggcgcctccg ggaatgaatg
     5461 ccaccattcc gatgaacggc gcaaggaaac ggacgtcttt ccaaggcaga tgcttgaacc
     5521 agccgaacag ccctttatgg ccttttcttc gaccagtcgt ttcaaacgtt gcaaagatgg
     5581 agaatgctgt catcaacgtc gggatgacaa ccatgaacgt caatacgact tggatgaatt
     5641 tccatgtcgg atcgattccg ggctctgtca attggtggtg gaagccgaca ggaatcgaga
     5701 acatgagcaa taaaatgaac gaaagccttg caagcgagtc actgaataat ttgccgccga
     5761 taattttcgg gatgattgca taccatgcca tatatgccgg aagcagccag aaatagacga
     5821 gcgggtggcc gaaataccag aacagcgtac ggctcagcaa cacgttgatt gtagctgcgt
     5881 agcccaatga ccaaggaatg aattgaatta agacggatga tgcaacacca agggatgcga
     5941 tgaaccacat cgccatattg atgacaacca tgaacgcgag cagcggagat ttctggcccg
     6001 gattcgcttt cttccagacg tacagctgac ggaagttgac gaatacggca atccagcttc
     6061 cgacgatgac cagcgccaaa ccgatataaa acgcggcatg tgcacgcagt ggagcataga
     6121 atgtgtacag gacattcgcc tggccgacca gaatggtgat tgctgcgata atcgtaccga
     6181 cgagcatcgt ccagaatgca atccatgccc attgacgttg cttgtcagac atgccgaccg
     6241 ttttccccat gagggcgaac tggaagccta cgatgaagaa tgtagtcaac accaaaccta
     6301 gaatgacacc atgaaccgtt aaaatggtat agtagtttat cccgaacggc agttggaatt
     6361 gccctgagcg ggacagtgtc tgcaagagcc ccatcaggcc gccgatcagc aaagacgcga
     6421 acgtcacatg catgaacgac atatagagcc gcgcttcttt cttaggaaac agaacgtttt
     6481 tcaacatcac tcaaacacct ccacagttgc aaacatttga tggtggccga ttccgcaata
     6541 ttcattgcag acgaccgtga attcaccggc gtttttcatg accgtttcaa ggcggctcac
     6601 atgccccggc tcgaccatca tgttgacatt cgtgcctgca acttggaagc catgcacgac
     6661 gtccttagta gtgatctgga ataaaacagt ggaacctttt ggcacgcgga tcttcttgac
     6721 gggctttccg tctgcatcct tgccgaaatc atagttgaaa gcagaagcga cgatattgac
     6781 tgtatatttc ccttctgcca cttcacgcag gccgaggttt tcaggtttga aagattcatg
     6841 tgcctcgacg ttctgcggat cgatcgtttc gatgtggctc atcggatgcg tccctttcca
     6901 gaaagccgcg aaaccgataa tgattagaaa aagtgcaagc gaacccaatc cg
//
LOCUS       DASULI010000362         5455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1100106,
            whole genome shotgun sequence.
ACCESSION   DASULI010000362 DASULI010000000
VERSION     DASULI010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100106"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..1872)
                     /gene="ahpF"
                     /locus_tag="VFX34_07770"
     CDS             complement(343..1872)
                     /gene="ahpF"
                     /locus_tag="VFX34_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEX5564842.1"
                     /translation="MILDADIKQQLAQYLELMEGDILLKVSAGEDKVSREMLSLVDEL
                     ATMSSRIQVEHAELERTPSFSVNRIGEDTGVVFAGIPLGHEFTSLVLALLQVSGRAPK
                     VDKKVIEQVKKIEDKLHFESYISLSCQNCPEVVQALNLMSVLNPNISHTMIDGAAYKE
                     EVESKNILAVPTVYLNGEPFTNGRKTIEEILAELGGGPDASEFEDKDPFDVLVVGGGP
                     AGASAAIYAARKGIRTGIVAERFGGQILDTASIENFISVNRTEGPKLAASLEEHVKDY
                     KIDVMNLVRAKRLEKKDLIEIELENGAVLKSKSVILSTGARWRNIGVPGEAEFRNKGV
                     AYCPHCDGPLFEGKDVAVIGGGNSGVEAAIDLAGIVSHVTVLEFNDELKADSVLQERL
                     HSLQNVTVITNAQTKEITGTDNVNGITYIERESGEEKHIELAGVFVQIGLVPNTDWLG
                     DSVERNKFGEILVDKRGATNVPGVFAAGDCTDSPFKQIIISMGAGATASLGAFDYLVR
                     N"
     gene            complement(2055..2624)
                     /gene="ahpC"
                     /locus_tag="VFX34_07775"
     CDS             complement(2055..2624)
                     /gene="ahpC"
                     /locus_tag="VFX34_07775"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEX5564843.1"
                     /translation="MSLIGKEILPFKAQAYHSGTGEFIEVTDENLKGKWSIVCFYPAD
                     FTFVCPTELGDLQDQYATLKELGAEVYSVSTDTHFTHKAWHDHSETISKLEYIMIGDP
                     SQTISRNFDVLDEESGLADRGTFIIDPDGVVQTVEINAGGIGRDASTLVDKIKAAQYV
                     RNNPGEVCPAKWKEGEETLKPSLDLVGKI"
     gene            3229..3612
                     /locus_tag="VFX34_07780"
     CDS             3229..3612
                     /locus_tag="VFX34_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5564844.1"
                     /translation="MNSIASPIACKVNNVFIHVSNLERSVEWYSNLLGLPFLKENVSS
                     PVHNIPVTSETGLTLDDHTFDPGFTLKPSDHVLFNFFVNDIDEAYDFIHNKGIKIVKE
                     IERIGDFAYFNFQDLDGNVLMICNC"
     gene            complement(3763..4701)
                     /locus_tag="VFX34_07785"
     CDS             complement(3763..4701)
                     /locus_tag="VFX34_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEX5564845.1"
                     /translation="MASGETEGSVKRFPFLLATMKGISQVLLIENWVTGLLILTAITI
                     TSLQLGLMAFLSAAIGTLFGRLGGGNPSSVERGLYGYNPMLTGMALFLFLEGPTSWGI
                     ALFGAVISAAFTASLMRFLQPAGLPTLTFPYIVTTWLLMLSSYRFEAFTLTDELVPQN
                     VSHWKLEIAGEIHWASAFLDGAGQIFFLQGIAAGLLLFLGIFIADWRMGVYALVGNFI
                     GMVTAYMLGGEHTLIDAGLYGYNAILTALAVGVVFRDGANRLTLFNVIIACVLTVVFI
                     GSVDTVLLPFGLPALTMPFAMSTSIFIGARMMMRNA"
     gene            complement(5005..>5455)
                     /gene="thiW"
                     /locus_tag="VFX34_07790"
     CDS             complement(5005..>5455)
                     /gene="thiW"
                     /locus_tag="VFX34_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEX5564846.1"
                     /translation="AVAGAAFVSFPAGIARAYPIQHAVNVIAAILLGPIPAVIIAFMT
                     GLVRILTGTGSLLAFPGGMIGAALAGLWYKQSGKAWLAALGEIIGTGVIASLFAVPYA
                     AVLMGTTVTAFFFMPPFLVSSVSGAVIGVVVAFRLQALPVGRRIRSY"
BASE COUNT         1449 a         1421 c         1128 g         1457 t
ORIGIN      
        1 cagtgacagt cccttcatta gcctcattat ttgccgttcc ttcattttcc gtcgccgggg
       61 ccgaatcatc tttcccatcg gcgcatgccg cgaaaattaa agcacaggta agtatcattg
      121 caagccaaat ccatcttttc atttccacac gctccctcta gtaaattatc gacacttata
      181 caattcacta ttttgtgtgt cataaaacta gattcacgta aaagagccct taccgaccac
      241 ctaaaatggg acgtattgac aacaaaccga gcaggaattc ctacaaaaaa gaacaggtac
      301 aatttcagac ctgttccttt tcactctatg aatgttcctc aattaattcc gaacgagata
      361 atcaaacgcg cctaacgaag cggtcgcgcc tgctcccatc gaaatgatga tctgcttgaa
      421 cgggctatcc gtgcagtcgc ccgcagcgaa tacgccaggg acgttcgtcg cgccgcgctt
      481 gtcgacgagg atttcaccga atttattgcg ctcgactgaa tcgccaagcc aatccgtatt
      541 cggcacaaga ccgatttgaa cgaatacgcc agctagttcg atatgctttt cttcaccgga
      601 ctcgcgctca atatacgtga tgccgtttac gttgtccgta ccggtaattt ctttcgtctg
      661 tgcatttgtg atgaccgtga cattctgcaa gctgtgcaga cgttcttgta aaacggaatc
      721 cgccttcaac tcgtcgttga actcaaggac cgttacgtgg ctgacgattc ctgcaaggtc
      781 aatcgccgct tcgacaccgg agttcccgcc gccgataacc gcaacatctt tcccttcgaa
      841 taaaggaccg tcacagtgcg ggcaatacgc aacgccttta ttcctgaact cggcttcgcc
      901 aggtacgccg atatttctcc agcgtgctcc cgttgaaagg atgacggatt tactcttcag
      961 aacagcaccg ttttccaatt cgatttcaat caagtctttc ttctcaagac gtttcgcacg
     1021 gaccaagttc atcacgtcga tcttatagtc tttcacgtgc tcctcgaggc tagcagctag
     1081 tttcggacct tccgtacgat tcacgctaat gaagttctcg atcgaagccg tatccaagat
     1141 ctgaccgccg aaacgctcag cgacaattcc tgtgcggatc cctttacgag ccgcatagat
     1201 tgctgcactc gcacccgcag gaccgcctcc gacaacaagg acatcaaacg gatctttgtc
     1261 ctcaaattcc gatgcgtccg gaccgccgcc gagttctgca aggatctctt cgatcgtctt
     1321 acgaccgttc gtgaacggct cgccattcaa gtaaaccgtc gggacagcaa gaatgttttt
     1381 gctttcgact tcttctttat acgccgcgcc gtcgatcatc gtgtgggaaa tattagggtt
     1441 caacacactc ataaggttaa gtgcttggac aacctcagga cagttttgac agctcaggct
     1501 aatataggat tcaaagtgaa gcttgtcctc gattttcttc acttgctcga tcactttctt
     1561 atccactttt ggcgcccttc cgctcacttg caataaagca aggaccagtg aagtgaattc
     1621 gtgtccaagc gggatacctg cgaacacgac gcctgtatcc tcgccgatgc ggttgacgct
     1681 gaagcttggc gttctttcca actcagcatg ctccacttga atgcgggatg acattgttgc
     1741 cagttcatcc acaagcgaca gcatttcgcg cgacacctta tcttctccag cgcttacttt
     1801 cagaaggata tcgccttcca tcaattcaag atattgtgct aattgttgtt tgatatctgc
     1861 atctaaaatc atgtaatcta ctccttaact tgtattttcc ccatagaaaa ccggcatgat
     1921 tgccttagat tttgaatcga taaaaccgga aattcgatcc aaaatctaag acatcagccg
     1981 aaaatattca aaaataaagg gagcccctat cccttaaagg aagggggccg cccccgatat
     2041 tcgctatcgc gaaattaaat tttacctaca aggtcaaggc taggtttaag ggtttcttcg
     2101 ccctctttcc attttgcagg gcaaacttca cctggattgt tgcgcacgta ttgtgcagct
     2161 ttgattttgt cgacgagtgt gctcgcgtcg cgaccgattc cgccagcatt gatttcaact
     2221 gtttgtacaa caccgtccgg atcgatgatg aatgtaccgc gatctgcaag acctgattcc
     2281 tcatccaata cgtcgaagtt acgggagatc gtttgtgaag ggtcaccgat catgatatat
     2341 tctagcttgc tgattgtctc agagtgatca tgccaagctt tatgtgtgaa gtgcgtgtct
     2401 gtggaaacgg aataaacttc agcacctagc tctttaagag ttgcatactg gtcttgaaga
     2461 tctccaagct cagtcgggca aacgaatgtg aagtcagccg gatagaagca aacgatgctc
     2521 catttccctt ttaggttttc atcagtaacc tcgatgaact cacctgtacc gctatggtaa
     2581 gcttgcgctt tgaatggtag tatttctttt ccgattagag acataaaaga attcctccta
     2641 gtgttttttc tatagttagt ttaggtcatt tcgacaaaac ctaaaccgca gcaagaatta
     2701 tgttgcaatc tcttctgctt aactataata attctaatta catactcatt atcatataga
     2761 accatacttt tgtcaacaaa tacacttttc ttcaccataa acccttatac ctcaaatgtt
     2821 tcgggattaa tccaacctta tcgattccat attatttaca ggataataag attttcatcc
     2881 aaaccccaaa ttctcaatta cccatccacg ttatcgttta ttcaatattt gaaagataat
     2941 gaatacaaat aatataataa aatctattta ttgtcccttt aagggtgcct gccttttagg
     3001 gtgcctgtgc accacacatt tatggcaatt caacaaaagt catatatatg caggaagaag
     3061 ccaaccgatc ctttatctgg ttggcttcat catttatttt atgcaattgt agttcattaa
     3121 ctgaatagtt tgctgcacag gcacccacac aatttccaaa caatttagaa ggggatttta
     3181 gggatttgtt gaattgtaat aagaaatgag taaaggagtg gaatcgcaat gaattcaatc
     3241 gcttcgccta tcgcttgcaa ggtgaacaat gtttttatcc acgtcagcaa cttggaaaga
     3301 tctgtagaat ggtatagcaa tctgctcgga ttgccttttt taaaggaaaa cgtttcgtct
     3361 cccgttcaca acatccctgt tacttcagaa acgggcctca ctttggatga tcacactttc
     3421 gatcccggct tcacgcttaa gccttctgat catgtattgt tcaacttctt cgtcaatgat
     3481 attgatgaag cttacgattt cattcacaac aaaggcatca aaattgttaa ggaaattgag
     3541 cgcattggtg attttgctta tttcaacttt caggatttgg acggaaatgt attaatgatc
     3601 tgcaattgct gaatgaccgg aggctgtccg ctttcgtgcg ttttccttgc tttatgcgta
     3661 tccggataac aggaacaagg gttggaattc gacttcattt ccaacccctt tctattttga
     3721 taggtagcat ccacgcaatc ctttaaggca caaagagact tgtcaggcgt tccgcatcat
     3781 catccgtgcg ccgatgaaaa tcgaggtact catcgcgaac ggcatcgtca aggccggtaa
     3841 tccgaacggt aagagcacgg tatcgacact tccaataaac acaaccgtca aaacgcatgc
     3901 gatgatcaca ttgaatagcg tgagccggtt cgccccatcc cggaaaacga ccccgacagc
     3961 gagcgccgtc aaaatggcat tatagccgta cagccctgca tcgatgagcg tatgctctcc
     4021 gcctagcatg tatgcggtaa ccatgccgat gaaattaccg acgagggcat acacccccat
     4081 cctccaatcc gcaatgaaaa tccctagaaa gagcagcaat cctgcggcga tgccttgcag
     4141 aaagaaaatt tggccggctc catcaagaaa agcacttgcc caatggatct ccccggcaat
     4201 ctccagcttc caatgcgata cattttgcgg tactaattcg tccgtcagtg taaatgcttc
     4261 gaatcgatac gaagacaaca tcaagagcca tgttgtcacg atatacggaa aggtcaatgt
     4321 cggcagaccg gccggttgca gaaatctcat caacgaagca gtaaatgcag ccgaaatgac
     4381 tgcgccgaat aaagcaatcc cccaacttgt aggcccttcc agaaacagaa agagcgccat
     4441 tcccgtcaac atgggattat acccatacag cccccgttca acgctcgacg gattcccgcc
     4501 gcctaagcgt ccgaaaagcg ttccgattgc tgccgaaagg aacgccatca aaccgagctg
     4561 caaggaggtg atcgtgattg cagtcaatat gagcagaccc gtcacccaat tttcgatgag
     4621 cagcacttgg gagatacctt tcatcgtcgc cagaaggaaa gggaatcttt ttacgcttcc
     4681 ttcagtttca ccacttgcca tgccgatgct ctccctcttt tccctttttg cgtattcata
     4741 ccctaagtcg atgcgtcata atctcaacgg caatgctttt ttactaataa cttcgtatcc
     4801 ttctccccac cggcatattg tatcggtgcc ttgtatcggt gcctgtgcac cacacattta
     4861 tgacaattca ccaaaagtta tatatatccg cataaaagcg aaccaaagat tgatttggtt
     4921 ggcttccttc atttaattta tgcaattgta gtgtattgtc tgaatagtat ctcgcacagg
     4981 cacccaaaca actctaaaac aattctaata acttcgtatc cttctcccga ccggcaaagc
     5041 ttgcagccgg aacgccacaa caactcctat gactgccccg cttacgctgg agacgaggaa
     5101 aggcggcatg aagaagaagg cggtgaccgt tgtccccatc aggacggccg cgtacggaac
     5161 cgcgaagagg gatgcaatca ccccagtccc gatgatttcg ccaagtgctg ccaaccacgc
     5221 cttccccgac tgcttatacc agagccccgc aagcgcggcg ccgatcatcc ctcccggaaa
     5281 ggcgagcagt gaacccgttc cggtcaagat gcgcaccaag ccggtcatga aggcaatgat
     5341 gacggcggga atcggcccta gcaggattgc ggcgatgaca ttgactgcgt gctggatcgg
     5401 ataggctcgt gcaatccccg ctggaaacga gacgaaagca gctccagcca ccgca
//
LOCUS       DASULI010000363         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_868126, whole
            genome shotgun sequence.
ACCESSION   DASULI010000363 DASULI010000000
VERSION     DASULI010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868126"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VFX34_07795"
     CDS             <1..1261
                     /locus_tag="VFX34_07795"
                     /inference="COORDINATES: protein motif:HMM:NF027896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5564847.1"
                     /translation="HVNRAVSVAITKPMGPIHLNIPLREPLLIDFERKSKASTFVERI
                     HGEIELSAIQRQSLEALFSEANSGLIIAGELPVGFDKGAFWNFARKLQWPVLCDPLSN
                     LRSEVPEECRSLCIAHYDAILKSDLFKSNAVPDTVIRFGAQPVSKPLSLFLKKCRPSV
                     FIAVDESPEFRDSLGVVTHHLQCSPESIYDVPEQQGASSYQEKWSSAEEIAETETMKY
                     EGLAGDEGVIAKALFDMLPDGCDLISGSSMPIRDVDTFFCKTEKDITLFSNRGTNGID
                     GVVSTAFGVEAARKRPTYLLIGDLSFLHDVNGLIVSRFHQTNLTIIILNNDGGGIFSY
                     LPQASVENHFEELFGTPTGLTFEHIGAMYDAQYAAVHTVEEFQAELQKEKTNDVRIIE
                     VFTSRPLNVKAHRAYWSEVVKGLEGDE"
     gene            1254..2078
                     /gene="menH"
                     /locus_tag="VFX34_07800"
     CDS             1254..2078
                     /gene="menH"
                     /locus_tag="VFX34_07800"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HEX5564848.1"
                     /translation="MNELVPVRGLSIYVEMEGDPQLPAVVFLHGFTGTSATWREVRDE
                     MQGKYRTIAIDLTGHGKTTVPGKPERYSMEEQIEDLEALFEHLKLDSFHLVGYSMGGR
                     ISLAYTVRYPKRVQALILESASPGLVDEQARTERRAGDKALAGKIMEGGIPSFVDKWE
                     NIPLFESQKSLPAEKRLAIRNERLQQSVIGLANSLMGIGTGSQESYWEAIHSIQHPVF
                     LITGELDAKFVAIAQEMNDRSPTWRHNVVSRAGHAIHVEKPRLFATMIMEYLQGLQ"
     gene            2095..>2530
                     /locus_tag="VFX34_07805"
     CDS             2095..>2530
                     /locus_tag="VFX34_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5564849.1"
                     /translation="MTRQWETIRTYEDIKYEKYNGIAKVTINRPEVRNAFRPKTVMEL
                     IDAFSRARDDNSIGVIILTGEGEKAFCSGGDQSVRGHGGYVGDDEIPRLNVLDLQRLI
                     RVIPKPVVAMVAGYAIGGGHVLHVVCDLTIAADNARFGQTGPK"
BASE COUNT          684 a          531 c          682 g          633 t
ORIGIN      
        1 gcatgtcaac cgtgccgtct ccgtcgctat aactaaaccg atgggaccga tccatttgaa
       61 catcccttta cgggaaccgc tgctcatcga tttcgaaagg aaatcaaaag catcgacgtt
      121 cgttgaaagg atccatgggg aaatcgaact ttcggctatt caaagacagt cccttgaagc
      181 tctgttttct gaggcgaaca gtgggctgat aattgccggg gaattgcctg tcggatttga
      241 taagggtgca ttctggaatt ttgcgaggaa gttgcaatgg cctgtgctat gcgatcctct
      301 atcaaacttg aggtcagaag ttccagagga atgcagatca ttgtgcattg ctcattatga
      361 tgcgattttg aaaagtgact tgttcaagtc gaatgcagtt cccgacacgg tcatccgttt
      421 cggtgcacag ccggtttcga agccattgtc tttatttctt aagaaatgcc ggccgtcagt
      481 gtttatcgct gtggatgaat caccggaatt ccgggattcg cttggcgtag tgacacatca
      541 tctgcaatgt tcgccagagt ctatctatga cgtgccagaa cagcagggag cgtcctccta
      601 tcaggagaaa tggtcttctg cggaagaaat tgctgaaacc gaaacaatga agtatgaagg
      661 gctggcaggg gacgaagggg tcattgcgaa ggcgttattc gatatgctgc cggatggctg
      721 tgatctcata agcggcagca gcatgccgat ccgtgacgtc gacacgtttt tctgcaagac
      781 ggagaaggat atcaccctat tttccaatag gggaacgaac ggcattgatg gggtcgtttc
      841 cacggcgttc ggtgtcgaag cggcacgcaa aaggccaacg tacctgctta ttggtgatct
      901 gtccttttta catgacgtca atggtttgat cgtatcaaga ttccatcaga caaacttaac
      961 gattatcata ttgaataatg atggcggcgg aattttttct tatcttccgc aagcgagtgt
     1021 cgaaaaccat ttcgaagaat tattcggcac accgacaggc ttgacgttcg aacatatcgg
     1081 cgcaatgtat gacgcgcagt acgctgccgt tcatacggtg gaggaattcc aggcggaact
     1141 gcaaaaagaa aagacgaatg acgtccggat catcgaagtg ttcacgagca gaccgttgaa
     1201 tgtgaaggcc catcgcgcct attggtccga agtcgtaaag gggctggaag gtgatgaatg
     1261 agctggttcc ggtgcgcggg ctatccatct atgtcgagat ggagggagat cctcagttgc
     1321 ccgctgtcgt ctttctacac ggttttactg gaacttcagc tacatggaga gaagttcggg
     1381 atgaaatgca agggaagtat cggacaatcg caatcgactt gacggggcac ggaaaaacaa
     1441 ccgtacccgg caagccggag cgttattcga tggaagagca gattgaagat ctggaagccc
     1501 tttttgagca tttgaaattg gattcctttc atttggtcgg ctactcaatg ggcggccgca
     1561 tttccttggc ttatacagtg cgttatccaa agcgggttca ggctttgatc cttgaaagtg
     1621 catctccagg tttggtggat gagcaagccc gaactgaacg aagagccggg gataaagcac
     1681 tcgctggtaa gataatggag ggaggaatcc cctcttttgt tgataaatgg gaaaatattc
     1741 ccctttttga atcccaaaaa agtcttcctg cggaaaaaag gctagcgatc cggaatgaaa
     1801 ggctgcaaca aagtgttatc ggacttgcaa acagcctgat gggaattgga actggcagcc
     1861 aggaatctta ttgggaagcc attcattcga tacaacatcc tgtttttctt ataacaggag
     1921 agctggatgc gaaatttgta gcaattgccc aggaaatgaa tgaccgatcc cctacatggc
     1981 gacacaatgt cgtttcacgc gctggacatg caattcacgt ggaaaaaccg cgattgtttg
     2041 ctacaatgat tatggagtat ttgcaaggtc tacaataatt ggaggaggaa gaaaatgaca
     2101 cgtcaatggg aaacaatccg tacatatgaa gatattaaat atgagaagta caacggcatt
     2161 gcaaaagtga caatcaaccg tccggaagtg cgcaatgcat tccgtcctaa aacagtaatg
     2221 gaattgatcg atgcgttttc tcgagcacga gatgataaca gcatcggagt catcatactg
     2281 acaggtgaag gcgagaaggc attttgctca ggaggggacc aatctgtccg cggacatggt
     2341 ggatatgtcg gagacgacga aattccacgt ttgaatgtcc ttgatttgca aagattgatt
     2401 cgtgtcattc cgaagcctgt cgttgcgatg gttgcgggat acgcaattgg cggcggacat
     2461 gtattgcacg tagtgtgtga cttgacgatt gcagcggaca atgctcgttt cggacaaaca
     2521 ggtccgaaag
//
LOCUS       DASULI010000364         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1644540,
            whole genome shotgun sequence.
ACCESSION   DASULI010000364 DASULI010000000
VERSION     DASULI010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644540"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="VFX34_07810"
     CDS             <1..2070
                     /locus_tag="VFX34_07810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5564850.1"
                     /translation="LKAKLYIDRVNNRLLASLEFQYDHVVLNPVTESDLPAGSVLVRD
                     LEKEEAILRLMEDSSFATTDEGYLLHNEELEYEFLHHALPKLQKLVQVYATTAIRNRI
                     FRGHAKPRIRVKAHEDRTNWLEFKFEMDGIPDKQIREILEALEEKRKYYRLRNGSLLS
                     LETREFEEIQRFLNEIPEDLETLEKGLNIPMVRGLRFLDAVGDDDMFSVEASARQFFD
                     RLLNPDDCAEVPASVDAVLRDYQKTGFQWMKTVAEFGVGGVLADDMGLGKTLQSIAYI
                     LSVLPDIREKKRQVLIVCPSSLTYNWLSEFRKFAPEINAVVMDGSKKERAERQSDVVD
                     KDVLITSYPLIRRDIQWYEKQDFLTIFFDEAQAFKNPFTQTARAVKRLQADTCFALTG
                     TPIENSEEELWSIFHVVFPELFMGLREYSELTAKAISRRVRPFLLRRMKEDVLGELPA
                     KVESIESSELLPEQKKLYAAYLAKLRHDTLKHLDKDTIRRNKIKILAGLTRLRQICCH
                     PALFVDGYQGSSAKFEQLKQLIEESKLAGRRVLIFSQFTKMLGIIGKELAMKGTPFFY
                     LDGQTPSEERLELCNRFNAGERDVFLISLKAGGAGLNLTGADTVILYDLWWNPAVEEQ
                     AADRAHRMGQQQNVHVIKLVARGTIEDKMNELQEKKRSLIEEIIDSKDTANTTLTEDD
                     IRELLEI"
     gene            2107..2532
                     /locus_tag="VFX34_07815"
     CDS             2107..2532
                     /locus_tag="VFX34_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564851.1"
                     /translation="MRAIKILLSIAVVIVVIGFGVYHFGTKFIADEVMGQVAAELESS
                     GQMEAIREEVQKDPELRAFIEEGKNVDTSKLPFQTKEEATRVLLKKFDLSEINDIQIQ
                     ARQGMTASEQQALFNKIESRLTDEEMLALKALAYKELFK"
BASE COUNT          734 a          561 c          737 g          588 t
ORIGIN      
        1 ttgaaggcga agctttacat cgatcgggta aacaaccgat tgcttgcaag cctcgaattc
       61 caatacgacc atgtcgtcct gaatccggtt acggaaagcg atttaccggc gggatccgta
      121 ttggtccgcg atttggaaaa ggaagaggcc atccttcgtt taatggaaga cagctcattc
      181 gccacaacgg atgaaggtta tttattgcac aatgaggaat tggaatatga atttctccat
      241 cacgccctcc cgaagcttca aaagcttgtc caagtgtacg cgacaacagc aatcaggaac
      301 cggattttca gggggcatgc caaaccgcgg atccgggtga aagcgcatga ggatcggacg
      361 aattggctgg aattcaaatt cgagatggac ggcatccccg ataagcaaat acgtgaaata
      421 ctagaagcgc tggaggaaaa acggaagtat taccgtttgc ggaatggctc tcttctttca
      481 ttggaaacga gggaatttga agaaatccaa cggtttttga atgaaatccc ggaagatctt
      541 gagaccctcg agaagggttt gaatataccg atggtgcgcg gcctccgctt cctggatgca
      601 gtcggtgacg atgacatgtt ttccgttgag gcatcggccc gccagttttt tgaccgtctg
      661 ctgaatcccg acgattgcgc ggaagtgcca gcgtcggtgg atgcggtatt acgcgattat
      721 caaaaaacag gttttcaatg gatgaaaacg gtcgctgaat tcggcgtcgg cggggtgctc
      781 gcggatgata tgggactcgg caaaacattg cagagcatcg catacattct gtccgtgctg
      841 cctgacatcc gcgagaagaa acggcaagtc ttaattgtct gtccctcttc tttaacgtat
      901 aactggctga gtgaattccg gaaattcgca ccggaaatca atgccgtcgt catggacgga
      961 tcgaaaaagg agagggcaga gcggcaaagt gacgtggtgg acaaggatgt gttgatcacg
     1021 tcctacccgt tgatacgccg ggatatccaa tggtatgaga aacaagactt cctgacgatc
     1081 tttttcgacg aggcgcaagc atttaaaaac ccgtttacgc aaacggcacg ggctgtgaaa
     1141 agattgcagg cagacacctg tttcgcgctg accggaacgc cgatcgaaaa ttcggaggag
     1201 gaactttggt cgatctttca cgtcgtattt cctgaactgt tcatggggct gcgggaatat
     1261 agcgaactta cggcaaaagc gatatcccgc cgcgtgcggc catttttgct gaggcggatg
     1321 aaggaagatg ttctcggaga gctgccggca aaagtcgaat cgatagagtc atcggagcta
     1381 ctgccagaac agaagaagct ctatgccgcc tatttggcaa agctgcgcca cgacacgtta
     1441 aagcatttgg ataaggatac gattcgaagg aataaaatca aaatcctcgc cggactgacc
     1501 cggctgcggc aaatctgttg ccatccggca ttattcgtcg acggctacca gggcagttcc
     1561 gcgaaattcg agcagttgaa gcagctgatt gaagaatcga aactagcggg aagaagagtg
     1621 ctgatctttt cgcaatttac gaagatgctc ggcatcatcg gcaaggagtt agcgatgaag
     1681 ggaaccccgt tcttttattt ggacggtcaa acgccttccg aggaacggtt ggaattatgc
     1741 aatcgattca atgcagggga gcgcgacgtt ttccttattt cattgaaagc gggcggagcc
     1801 gggttgaact tgacaggggc ggataccgtc atattgtatg acctttggtg gaaccccgcg
     1861 gtggaggagc aagccgccga ccgcgcccac cgcatgggac aacagcagaa cgtgcatgtc
     1921 atcaaactcg tcgcgcgcgg cacgattgaa gataaaatga atgaattgca ggagaagaag
     1981 aggagtctga tcgaagagat cattgattcg aaggatactg cgaatacaac gttgactgag
     2041 gatgatattc gggaactgtt ggaaatatag gaagcggtaa atactaatga aaaaggaggg
     2101 aattaattga gagcaataaa aattctacta agcattgccg tcgtaattgt cgttatcggc
     2161 ttcggggtgt atcatttcgg gacgaaattc atcgccgacg aagtgatggg ccaagtggcg
     2221 gctgaactcg aatcgagcgg ccagatggaa gcgatacggg aggaagttca aaaggacccg
     2281 gaattgcgcg cgttcataga agaaggaaag aacgtggaca cctcgaaact tcctttccaa
     2341 acgaaagagg aagcgacccg ggttttgctg aagaaattcg acctcagtga aatcaatgac
     2401 atacaaatcc aagcgcgaca aggcatgacg gcatcggagc aacaagcact gttcaataaa
     2461 attgagagcc gattgacgga tgaagagatg ttggcgttga aagctttggc gtataaagag
     2521 ttgttcaagt gaaaaaccgc ttcagtcagg tgtgactgga gcggttttta tggtggccgg
     2581 ggagttatca gtcactttcc aagggttatc gtcatttcaa
//
LOCUS       DASULI010000365         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1898444,
            whole genome shotgun sequence.
ACCESSION   DASULI010000365 DASULI010000000
VERSION     DASULI010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898444"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFX34_07820"
     CDS             <1..328
                     /locus_tag="VFX34_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX5564852.1"
                     /translation="AQQNAVEAGFMDLIRFEQCDVKDLTVEGLNGVLVGNPPYGERLG
                     EIEEAEELARVIGHIMDRYPSWSVYMLSSLENFEAMYGKKATKKRKLFNGFIRTDFYQ
                     YWGQRK"
     gene            397..2313
                     /locus_tag="VFX34_07825"
     CDS             397..2313
                     /locus_tag="VFX34_07825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX5564853.1"
                     /translation="MKRKMPFSLTKEKSFYESLNDWIGDTLYDDLTEKGFECRDEQIY
                     MAFQIEQALKEKKVLLAEAGVGTGKTIAYLLPAIAYARYTGKPALISCADETLIDQLV
                     KEEGDIRKISDALQIDIDVRLAKARDQYLCLKRLDEAVDSSMEDFAENVHGSLPDFVN
                     GNVSLQSVFPYGERSDYPHLNDEQWKTMNFHPIQQCAACDIRNRCGQTIHRNHYREAA
                     DLIICSHDFYMEHIWTKESRKRQGQLPLLPDVSMIVFDEGHLLEYSAQRALTYEVQNH
                     TLLNLLERIMVDGVREETLQLMERLIDVHEHFFRLLKTATKESEDERKAIQKNEETLS
                     VGREAISISTALLEEFVFEGELYIIPEYDLKMVEEYLEQYIYSMELFVSENDAVDWLQ
                     ESEDGMTLTIMPRLVTDILKEKLFSSNMPIVFSSATLSVGQDFSYILDSLGIDDFLSF
                     SVESPFDYEEKMEVIAADLDEGEKPKYVLDLLQDDEQTLLLFKSEQSMKKFQAELDEE
                     QRTGIAFEGERELSSIIREFQQKEISVLCSYHLWEGLDIPQDALTRVVIYDLPFPPHD
                     PLFDARRKHAENPFEDVDLPFMLLRLRQGAGRLIRTSEDYGVVHILLEGFEQEMKEVI
                     DGIFPVDLQTFVKK"
     gene            2454..>3210
                     /locus_tag="VFX34_07830"
     CDS             2454..>3210
                     /locus_tag="VFX34_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX5564854.1"
                     /translation="MFTNEILEQTAIYGHLFNKNHDEERVKKAASLAKKVMDEEFVIG
                     FAGHFSAGKSSMINALTGEDFLPTSPIPTSANIVKVKKADEEFAIIHRTDGSLVKYKG
                     HRFFDAIKSFSKDGAEISLIEIGHPGSKLPEAITVMDTPGVDSTDDAHRLSTESALHL
                     ADLVFYTMDYNHVQSELNFKFTKELLRYNSNVYLIINQIDKHRESELPFEEFKKSVED
                     SFKLWGVEPKGIFYTSLKSDDLPINDFEKVKGIV"
BASE COUNT          972 a          628 c          770 g          840 t
ORIGIN      
        1 tgcacagcaa aacgcagtgg aagcgggctt catggatctg atccgtttcg agcagtgcga
       61 cgtcaaagat ttgactgttg aaggtttgaa cggagtgctt gtcggcaatc ctccttatgg
      121 ggaacgattg ggtgaaatcg aggaagccga ggaacttgct cgcgtcatcg gtcatataat
      181 ggataggtat ccatcatggt ccgtttacat gctatcctca ttggagaact ttgaagcaat
      241 gtacgggaaa aaagcgacga agaaacgtaa gcttttcaac ggattcatca gaacagactt
      301 ttatcaatat tggggtcaac gtaaataagc aatcagatta gacggaagag gtcctgcctc
      361 ttccgtttcc atgtgtgaaa agaaagggga gcgcaaatga aaaggaaaat gccattttca
      421 ttaacaaaag aaaaatcatt ctatgaatcc ttgaatgact ggattggcga tacattatat
      481 gacgatttga ccgaaaaggg attcgaatgc agagatgaac aaatctatat ggcatttcaa
      541 attgaacaag cgctgaaaga gaagaaagtc cttcttgctg aggcaggcgt tggaacaggg
      601 aagacgattg cctatctatt gccggctatt gcttatgcaa gatatacggg gaaacccgcc
      661 ctcatttcct gtgcggatga aacgttgatc gaccagctcg tcaaagagga aggggatatc
      721 cgtaaaataa gcgacgcatt gcaaatcgac attgatgtcc gtctcgcaaa agcgagggat
      781 caatatttat gcctgaagcg attggatgaa gcagtagact cttcaatgga ggactttgca
      841 gaaaatgtcc atggcagctt gccggatttc gtcaacggca acgtctcgct gcaatcggtc
      901 ttcccgtatg gagaacgatc agactatcct cacttgaacg acgagcaatg gaaaacgatg
      961 aacttccatc ctattcaaca atgtgcggca tgtgatatcc gaaatcgatg cggccagacg
     1021 atccaccgga atcattatcg tgaagcggct gatttgatca tttgctcaca tgacttctat
     1081 atggagcata tttggacgaa ggaatcgagg aagcgtcaag ggcaactccc gttgctgccg
     1141 gatgtttcga tgattgtgtt cgatgaaggg catcttctcg aatattcggc acagcgagca
     1201 cttacgtacg aagtgcaaaa ccatacacta ctcaatctgc tggaaaggat catggttgac
     1261 ggtgtcaggg aagagacatt gcaactgatg gaacgattaa ttgatgtcca tgaacacttt
     1321 ttccgtctgc tgaaaacggc aacgaaagaa agcgaagatg aacggaaagc gatccagaaa
     1381 aacgaggaaa ccctttcagt tggaagggaa gcaatctcca tctcaaccgc attgctggaa
     1441 gagtttgttt ttgaaggtga attgtacatc attcctgaat acgacttgaa aatggtcgaa
     1501 gaatatttgg agcaatatat ctattcgatg gaattattcg tgtctgaaaa tgacgctgtt
     1561 gactggctgc aagaaagtga agacgggatg acactaacaa tcatgccgag gcttgtcacg
     1621 gatatattga aagagaagtt gttctcttcc aatatgccaa tcgtcttctc atcggctacg
     1681 ctttctgtcg gacaggattt ctcctatata ttggatagcc ttggcatcga tgatttcctt
     1741 tcattctcag tggaatcgcc ttttgattat gaagagaaga tggaagtcat cgctgcagat
     1801 cttgatgaag gagaaaagcc gaaatatgtg cttgatttgc tgcaagatga tgagcagaca
     1861 ttactgttgt tcaaatcgga acaatcgatg aagaaattcc aggcagagct ggatgaagag
     1921 caaagaacag gaatcgcttt tgaaggcgaa cgggaattgt cgtcaatcat ccgtgagttc
     1981 caacagaagg aaatttcagt cctgtgttct tatcatttat gggaaggcct tgatattccg
     2041 caagatgcac ttacacgtgt agtcatctat gacctgccgt tcccaccaca tgatcctcta
     2101 ttcgacgcgc gcaggaagca tgcggaaaat ccgtttgaag atgtggattt gccatttatg
     2161 ctgcttcgcc tcagacaagg agccgggcgc ctcattcgta cttcggaaga ttacggggtt
     2221 gttcatattc ttttggaagg ctttgaacag gaaatgaaag aagtgatcga cggaatcttt
     2281 ccggtggatc ttcagacttt cgtcaagaaa taaatgataa aagctgtctt ctattcgagg
     2341 gaactcgatc aaagacagct tttcctatac ataaacttcg aattttcaat ataaatcgat
     2401 tcattaagaa aaatggtata gtggaatgag aatgttagga aggatcgaat attatgttta
     2461 ctaatgagat tttagaacaa acagctattt acggtcatct attcaacaaa aatcacgacg
     2521 aagaacgtgt taagaaagca gcttccttag caaagaaggt aatggacgag gaatttgtca
     2581 tcggattcgc cggtcatttc tcagccggca aatcctctat gatcaatgcg ctgactggcg
     2641 aggatttttt gccgaccagt ccaatcccga cgagtgccaa tatcgttaaa gtgaagaagg
     2701 ctgacgaaga gtttgccatc attcatcgga cggatggctc gctcgtcaaa tacaaaggac
     2761 atcgcttttt cgatgcaatt aagtcattca gtaaggacgg cgcggaaatt tcccttattg
     2821 aaattggaca cccaggatcc aagttaccgg aagcgatcac agtcatggat acgcccggag
     2881 tcgattcgac ggatgatgcc catcgattat ctacggaatc ggcgcttcat ctcgcagatc
     2941 ttgttttcta tacgatggat tataatcatg tccagtcgga actgaatttt aaattcacga
     3001 aagaattatt gcgatataac agcaatgtct atctgattat taaccaaatt gacaagcata
     3061 gggaaagtga attgccattc gaggagttca aaaagtcggt tgaagactct ttcaaattat
     3121 ggggagtaga accgaaaggc atcttctata catctttgaa atccgatgat cttccaatca
     3181 atgactttga gaaggtgaaa ggcattgtcg
//
LOCUS       DASULI010000366         3273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_203794, whole
            genome shotgun sequence.
ACCESSION   DASULI010000366 DASULI010000000
VERSION     DASULI010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203794"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFX34_07835"
     CDS             complement(<1..476)
                     /locus_tag="VFX34_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX5564855.1"
                     /translation="MIRSMTTAVNTMNQLQGQFDIIGNNLANSATNGYKSSEANFHEL
                     LYQQFNNDKADTAARQSPAGIRYGVGATLGPAKMNWTVGSLQMTGRELDFALTEPKQY
                     FNLIMPGEDGEQTVYTRAGNFYVSPVEGGNLMLVNGEGYPVANSAGQPITFADTVK"
     gene            complement(502..1338)
                     /locus_tag="VFX34_07840"
     CDS             complement(502..1338)
                     /locus_tag="VFX34_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HEX5564856.1"
                     /translation="MFRGFYTVGSGMIAQQRRTEMLANNIANANTPGFKAEQSSIRSF
                     PEMFMSSLNAQRIPTQKGIQLNGLSPVGELSTGVYMQETMPLFTQGGLRETGMTTDIA
                     LLDGSLPIDPETGEPGAVFFRLENENGNELYTKNGNFTIDAAGYLTNPMGYYVLDTNG
                     QRIQLQNDDFSISDAGDIMQNGATVATLGVSFAEQPGALLKQGNGLFASDGNLPEAQN
                     MAGVGYSFQQGFLESSNVDAAKAMTDMLAAYRAFEANQKILQAYDRSMEKAVNEIGRV
                     NG"
     gene            complement(1401..2393)
                     /locus_tag="VFX34_07845"
     CDS             complement(1401..2393)
                     /locus_tag="VFX34_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5564857.1"
                     /translation="MFAKDIGIDLGTANVLIHVKGKGIVLDEPAVVAIDKHTNKVLAV
                     GEEASKMVGRTPGNIIAIRPMKDGVIADFDVTEMMLRHFINKLNVKGFLSKPRILVCC
                     PTNITNVEQKAIQDAAEKSGGKKVYLEEEPKVAAIGAGMDIFQPSGNMVIDIGGGTTD
                     VAVLSMGDIVTSESIKSAGDLFDNDIIQFIKKKYKLLIGERTAETIKKEIGTVFPGGR
                     AEQMDIRGRDMVSGLPRTITIGSEEIREALSESVGMIVQSAKNVLEKTPPELSADIID
                     RGVFLTGGGALLHGIDQLLAEELRVPVFISDNPLSCVAIGTGILLDNIGKAARY"
     gene            complement(2759..3028)
                     /locus_tag="VFX34_07850"
     CDS             complement(2759..3028)
                     /locus_tag="VFX34_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HEX5564858.1"
                     /translation="MHEQIRRRCVRLGKMLLDTGLTVRALAKSTGYSKSTVHKDLTER
                     LPNVDVELSEEVAKILAYHKSVRHLRGGEATRMKWMNENKKARNT"
BASE COUNT          675 a          872 c          767 g          959 t
ORIGIN      
        1 tttttgacag tgtcagcgaa tgtgatcggc tgtcctgcgc tatttgcaac cggataacct
       61 tctccgttga cgagcatcaa gttgccgcct tcaaccggtg aaacgtaaaa attcccagca
      121 cgtgtgtaaa cggtttgctc cccgtcttca ccgggcatga taaggttgaa atattgcttc
      181 ggttcggtca acgcgaaatc gagttcgcgg ccggtcatct gcaaagagcc gactgtccag
      241 ttcattttag cgggaccaag cgtggcaccc acaccgtagc ggatgcctgc cggagattga
      301 cgcgctgccg tgtcggcttt atcgttattg aattgctggt agagcagttc atgaaaattg
      361 gcttcactcg atttataccc gtttgtagcg gaatttgcaa ggttatttcc gatgatatcg
      421 aattggcctt gcaattggtt catcgtgttc actgccgtcg tcattgatcg tatcattcgt
      481 ttctgcccct ttcaaaacgc atcatccgtt gacacgtcca atttcattca cagctttttc
      541 catgcttcga tcatatgcct gaaggatctt ttgattcgct tcgaatgcac ggtatgcggc
      601 aagcatgtcc gtcattgcct tcgccgcgtc gacattggag ctttccagaa agccctgctg
      661 gaatgaatac ccgacgcctg ccatattctg cgcttcaggc aagttgccat cgcttgcaaa
      721 caggccgttt ccttgcttca ggagagcgcc cggctgctcc gcgaatgaaa caccgagtgt
      781 cgcaactgtt gcaccgttct gcatgatgtc gccagcatct gaaatgctga agtcgtcatt
      841 ttgcagttgg atgcgctgac cgttcgtatc gaggacgtaa taccccatcg gattcgtcaa
      901 gtagccggcc gcatcgattg tgaaattccc gtttttcgta tagagctcat ttccgttttc
      961 attttcaaga cggaaaaaga cagcccccgg ttcaccggtt tccgggtcga tcggcaagct
     1021 tccgtccagt agcgcgatgt ccgtcgtcat gcccgtttca cgaagtcctc cttgtgtgaa
     1081 aagcggcatc gtctcttgca tgtagacgcc tgtcgacagc tccccgaccg gcgacaagcc
     1141 attcagctgg atgccttttt gagttgggat gcgttgcgcg ttcaggctgg acatgaacat
     1201 ttccggaaac gaacggatgc ttgactgttc agctttgaat cctggtgtat tggcgttcgc
     1261 gatattgttt gccagcattt ccgtcctgcg ctgctgcgcg atcatgccgg agccgactgt
     1321 gtaaaatcca cggaacataa ggcgacccgc ctcctttcat tcgttcttct attatttatt
     1381 tcggttgaat ttgtgattta ttaataacgt gccgctttgc cgatgttatc gagcaggatt
     1441 cccgtcccga tcgccacaca gctgagcgga ttgtccgaaa tgaacacggg caccctcaat
     1501 tcctcggcga gcagttggtc gatcccgtgc aataaagcac cgccgcctgt aaggaagacg
     1561 ccccggtcga tgatgtccgc cgatagctca ggcggtgttt tctccaaaac gtttttcgcg
     1621 gattggacga tcatgccgac cgattcgctg agtgcttccc gaatttcttc ggatccgatc
     1681 gtgatggtgc gcggaagacc gctgaccata tcgcgtccgc ggatgtccat ctgctctgcc
     1741 cggccgcccg gaaagacggt cccgatctct ttcttgatcg tttccgctgt ccgctccccg
     1801 atgagcaatt tatatttttt cttgatgaat tgaataatgt cattgtcaaa cagatcgcca
     1861 gcggacttga tcgattcgga tgtgacgatg tcgcccatcg acagcactgc gacatccgtc
     1921 gtgccgccgc cgatatcgat gaccatattg ccgctcggct ggaagatgtc catgccggca
     1981 ccgatcgccg ccactttcgg ctcttcttca agatacactt ttttgccgcc ggatttctcc
     2041 gccgcgtcct ggatcgcttt ctgctcgacg tttgtaatgt tcgtcgggca gcagacgagg
     2101 atccgcggtt ttgaaaggaa tcctttgaca ttcaatttat taatgaagtg acgcagcatc
     2161 atttctgtca cgtcgaaatc ggcgatgacg ccatctttca tcggacggat ggcaatgatg
     2221 ttgcccggcg tccttcctac cattttactc gcttcttcgc caaccgccaa cactttgttt
     2281 gtatgtttgt cgattgcgac gactgcaggc tcgtctagaa cgatgccttt cccttttacg
     2341 tgaatcaaca cattcgcagt ccctaggtcg attccgatat cctttgcaaa catcaatccc
     2401 gtttcccctt ccaacttcag ttcggacata ttctcctaac tatagattgt accataacag
     2461 ctctttaatg aacacaaata ttgacagtta ttttgcattt ttttatacag gaattcgaag
     2521 tctggggaat ggtgtcgaat gggggttttt tacaggggat tttggggagt cttaattttg
     2581 acaaccgttt ttgtctagtc ctttttcatt ccattctaaa agccatggat agattcatta
     2641 aacattccgt ccgcaattat tcggtttttt tcggcttccc gcctggaatt ttttgcatgc
     2701 gaaaagacag ggtgtccgcc ttcctgcaag gatggtcgat tggccccgtc tattcccgtc
     2761 acgtgttacg ggctttctta ttttcattca tccatttcat ccgtgttgct tcaccgcctc
     2821 ttaaatgtct gacagactta tgataggcaa gtattttggc gacctcttca gacagctcca
     2881 catctacatt cggaagccgc tccgttaaat ctttatggac cgtgcttttc gaatagcccg
     2941 tcgatttcgc cagcgcccgt acggtaagtc cagtgtccag caacattttg ccgaggcgca
     3001 cgcatcgcct ccgaatttgc tcgtgcacgc cctcttcctt tcctgtcagc tccgtttctt
     3061 cctttatcct atgcggctga cgggacggtt atgtcaaagg gggattgggg tgatggggtt
     3121 attctttttt atcccgtgat ttctgctata tcaatgtgat gatcgcagga taaatcccct
     3181 tctattcatg cagacagact cgcgcaaaat gcctttgaca attaaaaaaa ccactctcgc
     3241 aacggagagt ggttcgtttc attagaatcc tag
//
LOCUS       DASULI010000367         8098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1909276,
            whole genome shotgun sequence.
ACCESSION   DASULI010000367 DASULI010000000
VERSION     DASULI010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909276"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1561
                     /gene="proS"
                     /locus_tag="VFX34_07855"
     CDS             125..1561
                     /gene="proS"
                     /locus_tag="VFX34_07855"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5564859.1"
                     /translation="MSNQKNDFSKWYIDTIQKADLMDYTPVRGCIAFKPDGFEIWEHI
                     QAEMDKRFKETGHRNAYFPMLIPESFFQKEKDHIEGFSPELPFVTEAAGEKLEERLAL
                     RPTSETMIGHLYSDWIKSYRDLPVLINQWANVFRWEKRTLPFIRTSEFLWQEGHTAHV
                     DEEEARAETMQMLNIYKEVVEGLLAIPVYDGQKTPSERFAGAVDTYSIEAMMKDGKAV
                     QAGTSHYLGTKFAEAFDIKYLTKENRHEFVHTTSWGTSTRLIGSVIMVHGDEQGLVLP
                     PRIAPTQVVLIPVGPWKKNPAIMEKLDEVFAQLKAKGIRVRLDDSDQSPGFKFNEWEL
                     KGVPVRVELGPRDLENNQALMKARDEDEKVAVDLANIVESIEQELQTMQTRLLEKARA
                     FRAEHSHTHIDTMEELTKHIADSADKGEIPGWILAGWCGDDACEEQVKDETMFTTRNI
                     PFNPPAEKSTCINCGKEAKHTVWFARAY"
     gene            complement(1765..2499)
                     /locus_tag="VFX34_07860"
     CDS             complement(1765..2499)
                     /locus_tag="VFX34_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX5564860.1"
                     /translation="MENKKYLLATDLDGTFVGDKDALGRLLQFFDGSPDEVALVYVTG
                     RHLSSARLLMAEEGLPMPDLLITDVGTAIYRDVEEDLDWKARMQADWQPGMVAEIAAS
                     IPSLKKQKLPDDRRVSFTVSDDIGAVNEFKKALEDRAIAHSFVFSSNRDIDILPSGAG
                     KGNALEYALGRYAMEDVKLLIAGDSGNDRDMLSLGHPSVIVGNAQPELRELEPCENLF
                     WANANCAGGIYEAWLHFYGNRRESAG"
     gene            complement(2468..3685)
                     /locus_tag="VFX34_07865"
     CDS             complement(2468..3685)
                     /locus_tag="VFX34_07865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5564861.1"
                     /translation="MKKKIVFISDHGDPLALLGGEQAGGQNNYVKQLALSLDKRGHAV
                     DVITHWADAKAPKIEEFGSSCRVIRVSAGVKGYVPKSDMYNMLYDFYDDIQSQVDLTS
                     YDLMHTHYWLSGLLGAKLAEDYGLPWIHTSHSLGVAKEQATGITESKRIAAEQLILQL
                     ADTIVATTSSERKLINGFVEDPSPIKVIPIGVDKNFKPSIENHEVRPYFAFAGRLEVT
                     KGIYTLLNAFRLLMENRELPSSAKLIIAGGDPDRIDFERKLPTCPKLRKAVEGLERHV
                     DFIGARSQRQLAELFNDATAVIVPSTYESFGMVAAEAQACGSPVIASRVGGLKDVVRH
                     RETGLHVEKENKEHLAFAMKLLAANREFTRSLGRRAAAYARREFDWDTIAKKMDGLYE
                     VVILGKQKILASD"
     gene            complement(3835..5025)
                     /locus_tag="VFX34_07870"
     CDS             complement(3835..5025)
                     /locus_tag="VFX34_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX5564862.1"
                     /translation="MLKDRIYYQNAYKKTFKARIIGNGVDPKGRPYAILSNTAFYPTG
                     GGQPHDTGTIEGVRVIDVEEVDGEVRHFLDEELHSKKEVEGTIDWERRLDHMQQHAGQ
                     HILTAAFVRLFGFQTVSFHLGKGLVSIDLDAEDVTDKQLAAAEKLANEIILENRPIES
                     KWVTEDELSEHSLRKQLAVTDEIRLVIIPDFDTNGCGGTHPSSTGQIGLLKIISTEKQ
                     RRKVRVHFACGSRVLQQLHRKHEELSAASKLLSAPEDGVSHAIERLLASHHALEKSLE
                     NAREDVLSFEAQSLLDNKDGNIVKASFTNRTVQELQKLAKLIIAEDDSAIALLVAENE
                     DRLQFVAARGASSGTSMRLVSTSVLPLLNGKGGGNDAFVQGGGERTMAADELLNVMEN
                     IIVD"
     gene            complement(5090..5449)
                     /locus_tag="VFX34_07875"
     CDS             complement(5090..5449)
                     /locus_tag="VFX34_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564863.1"
                     /translation="MDIFMVIIDTLIVLLYITLIAFITPFTMKFFKRKNFDIFIQLLP
                     VCFIVFVSSIIISMGLGGFQEMLFFKITSIVSVVLLGFLLFLTLAMRVLWKDKYDMLV
                     ERILSLREKNERSFSLK"
     gene            complement(5586..6320)
                     /locus_tag="VFX34_07880"
     CDS             complement(5586..6320)
                     /locus_tag="VFX34_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564864.1"
                     /translation="MSKFDERIKKALEKHTDTDAHELKDDIWNEVDKELFERKRTKLK
                     RKKRWIPAVLIAAAALVLAFTLQTEPGLALMKGIKDMFAPEKEIIQSIEGQDEPTEVH
                     LNEGKDSEYIIYVDESRYKMIKGEDADIITTIEPLPDKYPEVSMEIKQYPSDKPEVLV
                     KELEEKLKVDFPDLREVETVSEPVEGFWLHGLTESTWDGKVIDVYVISNGKDGSFVIT
                     EKYFLEAAEGHGARFYHMLQSFEIIE"
     gene            complement(6320..6847)
                     /locus_tag="VFX34_07885"
     CDS             complement(6320..6847)
                     /locus_tag="VFX34_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010648541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5564865.1"
                     /translation="MLQEKWVRKIRAGNQAAFRQFYEAYANPAIRTASAITRNREMAK
                     DAVQETFIRVYRQIGSYNSALPFDPWFYRILTNECLRLMKKESPLFTVETLDNDSSIA
                     VESFDQLTELYDVIQALDDSHRIPLILKYIKGFSEKEIADILGLNQNTVKSRLFKGRK
                     RLKEQLEPTREEDMC"
     gene            complement(6972..7859)
                     /locus_tag="VFX34_07890"
     CDS             complement(6972..7859)
                     /locus_tag="VFX34_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5564866.1"
                     /translation="MKPWMYPLFVILGACSYGILSTIIKLAIADGYTAPQAVTSQYYT
                     GFLLAALIYLFTRRTLPKFGGGYTLVLAGLLTAITGTVYGKSVSLMPASLAVVMLFQF
                     TWIGMLFDCIARQRLPKRIEVISLVFLFGGTIFAAGVIDADLSGIPWQGWAWGMAAAV
                     SFSSFLMVNQRQVEGMDMLTRLLFMSFFAAVAITFFQEPQIAWDGTLLDGLWIYGLIL
                     GVFGIILPILLFSIGIPKVGTGMASILSAIELPVAVIVSMLLLHEHMTVLQIAGIVII
                     LIGMTLPSIANRRIRLKNA"
BASE COUNT         2128 a         2048 c         1840 g         2082 t
ORIGIN      
        1 acaaaacgtt cgagggctta gcatggctag gccgaatgaa ggtggtacca cgtctatcca
       61 gcgcaaagac gtccttgtat taggacagct ttgcgctttt tattatgata gggaggaaat
      121 caagatgagt aatcagaaaa acgatttttc aaaatggtat atcgacacga tccagaaggc
      181 ggatctgatg gactatacac ctgtccgcgg atgtatcgca tttaaaccgg acggcttcga
      241 aatttgggag catatccaag ccgaaatgga taagcgtttc aaggagacgg gccaccggaa
      301 tgcatacttc ccgatgttaa ttccagaatc gttcttccaa aaggagaagg atcatatcga
      361 agggttctcc cctgaacttc catttgtgac ggaagctgcc ggcgagaaat tggaagagcg
      421 cctagctttg cgtccgacgt ccgagacgat gatcggccac ttgtattccg attggatcaa
      481 aagctaccgt gaccttccag tgctgatcaa ccaatgggcc aacgttttcc gttgggagaa
      541 acggacattg cctttcattc ggacatctga gtttttgtgg caggaagggc atacggcgca
      601 tgtcgatgaa gaagaagcac gtgcagaaac gatgcaaatg ttgaatattt ataaagaagt
      661 cgtggaaggg ctattggcaa ttccggtcta tgacggacaa aaaacaccat ccgagcgttt
      721 cgcaggcgcg gtcgacacgt attcgatcga ggcgatgatg aaggacggga aagcggtaca
      781 agcaggtacg tcccactatt taggtacgaa gtttgcggaa gcattcgaca tcaagtattt
      841 gacgaaagaa aaccgtcatg aatttgtcca tacgacatca tggggaactt caacgcgtct
      901 catcggttca gtcatcatgg tgcacggcga cgagcaaggg cttgtattgc caccacgtat
      961 cgcaccgaca caagttgtct tgattcctgt tggaccttgg aagaagaacc ctgccatcat
     1021 ggagaagttg gatgaagtat tcgcacagtt gaaggcaaaa ggcatccgcg tacggttgga
     1081 tgattccgat caatcgccag gcttcaagtt caatgagtgg gagctgaaag gtgtacctgt
     1141 tcgcgttgaa ctaggaccac gtgatttgga gaataatcag gcgcttatga aagcacgtga
     1201 cgaagacgag aaagtcgctg tcgacttggc gaatatcgtc gaaagcattg agcaggaatt
     1261 gcaaacgatg cagacacgcc tacttgaaaa agcgcgtgca ttccgagcag agcattccca
     1321 tacgcatatc gacacaatgg aggaactgac gaaacatatc gcggattcag cggataaggg
     1381 cgaaatccct ggttggatcc ttgccggctg gtgcggcgat gacgcgtgcg aggagcaagt
     1441 gaaggacgaa acgatgttca cgacgcgcaa cattcctttc aatcctccag ctgaaaagtc
     1501 gacatgcatc aattgcggaa aagaagcgaa gcatacagtg tggtttgcga gagcttattg
     1561 atattttgaa tgaagcaggc tgccatccct cgggcggcag ccttttcgcg cgggtgaata
     1621 aaatttattg gagccgttta atggggattc agggctgtta gatggtcgag tggtgataac
     1681 atgcctctaa gtggtgataa actccttctg agcggtgata aagtgcctcc aagcggtgat
     1741 aaacatcttc tgagcggtga taactcatcc cgccgactcc cgccggttgc cataaaagtg
     1801 cagccaagcc tcgtagatac cgcccgcaca gtttgcattt gcccaaaaaa gattttcgca
     1861 tggttccaat tcccgcaact ccggctgagc atttcccacg atgacggaag ggtgaccaag
     1921 ggagagcata tcccgatcat tgcccgagtc gcctgcaatt agcagcttaa cgtcctccat
     1981 tgcatatctc ccaagcgcat attccaaggc attccccttg ccggcaccac taggaagtat
     2041 gtcgatatcc cggttcgagc tgaagacaaa cgagtgggcg atcgctcggt cctcaagagc
     2101 tttcttgaat tcattcaccg ctccgatgtc atctgataca gtaaaggaaa cacgccgatc
     2161 atcgggcagc ttctgttttt tcaatgaagg aatagatgcc gcaatctcag caaccatccc
     2221 aggctgccaa tccgcctgca tgcgtgcttt ccaatcaaga tcttcctcga catcccgata
     2281 aatggctgtc ccgacatccg ttataagcag atccggcatc ggcaatcctt cctccgccat
     2341 tagtagccgc gcggaggata aatgccttcc agtcacatag acgagtgcaa cctcatccgg
     2401 cgagccatcg aagaactgaa gcaatcgccc aagcgcgtcc ttgtcgccga caaatgttcc
     2461 atccaagtca gtcgctagca agtatttttt gttttccaag tatgaccacc tcatacaatc
     2521 catccatttt cttagcaata gtgtcccaat cgaattcccg ccgcgcatag gcagcagcac
     2581 gtcgtccaag ggaacgggta aattcgcggt ttgccgcgag cagtttcatg gcgaaagcca
     2641 aatgttcctt gttctccttt tcgacatgga ggcccgtttc ccggtggcgg acaacatcct
     2701 tcaacccgcc aactctagag gcgatgaccg ggctgccgca agcttgcgct tccgcggcga
     2761 ccattccgaa cgattcatac gtcgacggga caatgacggc agttgcatca ttgaaaagct
     2821 cggccaattg gcgctgggaa cgggctccga taaagtcgac atgccgctcc aatccctcaa
     2881 ctgcctttct cagttttgga catgtcggca atttcctttc gaaatcaatt ctatccggat
     2941 ctccgcccgc tatgatcagc tttgcagatg acgggagttc gcgattctcc atcaaaaggc
     3001 gaaaggcatt aagaagcgtg taaattcctt tcgtcacctc aagccttccg gcaaatgcga
     3061 aatatggccg cacctcgtga ttttcaatgg aaggcttaaa gttcttatca accccgatgg
     3121 gaatgacctt aatcggggaa gggtcttcaa cgaagccatt gatcaatttc cgctcagacg
     3181 aagtcgtagc aacaatcgtg tcggctagct gcaaaatcaa ttgttcagcg gcaatccgtt
     3241 ttgattccgt gatgccggta gcctgttcct ttgcaacacc tagtgagtgg gaagtgtgga
     3301 tccacggcaa cccataatcc tcagccaatt tcgcaccgag aagcccggat agccagtaat
     3361 gggtatgcat gagatcataa gacgtcaaat cgacctgact ttgtatgtca tcatagaaat
     3421 catagagcat gttgtacata tcgcttttcg gtacatagcc tttaactccc gccgatacgc
     3481 gaatcacccg gcagcttgaa ccgaattctt caatttttgg ggctttcgca tccgcccaat
     3541 gtgtaatgac atctactgca tgtcctcttt tatccagtga aagtgccaat tgcttaacat
     3601 aattgttttg tccgcctgcc tgttcgcctc cgagcaatgc aagcggatcg ccatgatcgg
     3661 aaatgaatac tatttttttc ttcatatgaa tctccctttc gaagctaaca ggaacatcct
     3721 ggtatctaat gtttttattt tcccttgatg tcaaatagta aacatactca gttggcaagc
     3781 tttattaaat aataaaaacc tcctgcgttg gaaatcagga ggagtcctta tcaattagtc
     3841 cacaattata ttttccatca cattcaacag ttcatccgcc gccattgtcc gctcgcctcc
     3901 cccttggaca aatgcatcat ttccgccgcc tttcccgttg agaaggggca agaccgaagt
     3961 ggaaactagt ctcatgctag tccctgatga tgcgcccctt gctgcgacga attgaagtcg
     4021 atcttcattc tcggcaacaa gaagggcgat ggcagaatca tcctcagcga taatcagctt
     4081 tgctaatttt tgcagttcct gtacggtccg atttgtgaac gaagctttta caatgttgcc
     4141 gtccttattg tccaataagc tttgtgcttc gaatgatagt acatcttccc ttgcattttc
     4201 caatgatttt tccaatgcat gatgggaagc caaaagccgc tcgatggcat gactgacacc
     4261 gtcttcaggg gcgctcagca atttggaggc ggcagacagc tcctcgtgct tccgatgcaa
     4321 ctgctgaagt acccggctgc cgcaagcgaa gtggacacgg actttccgtc tctgtttttc
     4381 cgtggagatg atcttcagca atccgatttg gcccgtcgag gacgggtggg tgcccccgca
     4441 gccgttcgta tcgaaatccg gaatgatgac gagtctgatt tcgtccgtca cggccaattg
     4501 tttccgcaat gaatgctcgg aaagctcatc ctccgtcacc catttcgatt caatcgggcg
     4561 gttttccaaa atgatctcat tcgcaagctt ttcagcagcc gctaactgct tatctgtcac
     4621 gtcttctgcg tccaaatcga ttgaaacaag ccctttgcca agatgaaaac tgactgtctg
     4681 aaatccgaaa agacggacaa acgcagctgt caatatatgc tgccccgcat gctgctgcat
     4741 atgatccagg cggcgctccc aatcgatcgt gccttcgact tcctttttgg aatgaagttc
     4801 ttcgtcaagg aaatgacgga cttcaccgtc cacttcctcc acatcgatta cccgtactcc
     4861 ttcaatcgtt ccggtgtcat gcggctgccc gccgcctgta ggataaaatg cagtgttgct
     4921 caatatggca tatgggcgcc ccttgggatc cactccgttc ccgatgatgc gtgctttgaa
     4981 agtctttttg taggcatttt gataataaat acggtctttt aacatactgc catcccctag
     5041 catgaatagt ttagtaacag tgtacaataa atagaaaagg gcttcccact tatttcagtg
     5101 agaagctccg ttcatttttt tcgcgtaagg aaaggattct ttcaactaac atatcgtact
     5161 tgtctttcca taatactctc atcgccaatg tcaggaataa tagaaatcct agtagaacca
     5221 ctgatacgat acttgttatc ttgaaaaaca gcatctcctg gaatccgcca agtcccatgg
     5281 aaatgatgat gctgctgaca aaaacaatga agcagaccgg cagcagttga atgaaaatat
     5341 cgaagttctt tcttttgaaa aacttcatcg taaaaggtgt gatgaatgcg atcagtgtta
     5401 tatataataa aacgatcagc gtgtcgatga tgaccatgaa gatatccata aataaaagcc
     5461 tccttgctat ctactattgc aatttatacc ctatatcctt ataacatatg cataataaaa
     5521 gaagaatcta cttttatacc tataaaaaaa gaaggggatt ataggctccc cctccgagat
     5581 tcagttcatt caataatttc gaagctctgc agcatatgat agaaccttgc cccatgccct
     5641 tcagctgctt ccaggaaata cttctcggta atgacaaagc ttccatcttt tccattactg
     5701 atgacataca catcaataac cttcccatcc catgtcgatt cggtcagacc gtgcagccag
     5761 aacccttcga ccggttccga aacggtttca acttccctta gatcgggaaa gtcgaccttc
     5821 agcttttcct caagctcctt cacgagaact tccggcttgt cagatggata ttgcttgatt
     5881 tccatgctca cttccgggta cttatcggga agaggttcga tagtggtgat gatatcagcg
     5941 tcttctccct tgatcatttt atagcgggat tcatccacgt aaatgatata ctcagaatcc
     6001 tttccttcat tcaaatgaac ttccgtcggt tcatcctgcc cctcaatgct ttggatgatc
     6061 tccttttcag gagcgaacat atccttgatt cccttcatca acgccaaacc cggctcggtc
     6121 tgtaaggtga acgcaaggac aagtgcagcc gccgcaataa gcactgcagg aatccatctc
     6181 tttttccttt tcaatttcgt acgcttcctt tcgaatagtt ctttgtcgac ttcattccaa
     6241 atgtcgtctt tcagttcgtg cgcatctgta tcggtatgct tttcaagcgc cttctttatc
     6301 cgctcgtcaa atttgctcat cagcacatat cctcctctcg cgtgggctcc agttgctctt
     6361 tcaaccgctt ccgtcctttg aataatcggg acttgacggt attttgattc aaccccaaaa
     6421 tgtccgcaat ctctttttcg gaaaaacctt tgatatattt caagatcagc ggaattcggt
     6481 gggaatcgtc caatgcttga atgacatcat acagctccgt caactgatcg aaagattcca
     6541 ctgcaatgga agagtcgtta tccagcgttt caactgtgaa aagaggggat tcttttttca
     6601 ttaaacgaag acattcattc gtcaatatcc gataaaacca agggtcgaaa ggcaatgcgg
     6661 agttgtagct gccgatttgc cggtacaccc ggataaacgt ttcctgtact gcgtccttcg
     6721 ccatctcgcg gttgcgggtg atggcgctcg ccgtccggat cgccggattg gcatacgctt
     6781 cataaaattg ccggaaagcg gcttggttgc ctgcccttat tttccggacc cacttttcct
     6841 gcagcacacg gcacactcct ttcagaacaa cctagtttgt tgctctcact atatataccc
     6901 ttaagtttgc agaaaagttt tcaatgcaac aaaaaaacca catcacttat ctgagtgatg
     6961 tggaaactgt ctcaagcgtt tttcaatcgt atccttctgt tcgcaatgct cggcagcgtc
     7021 atgccgatga ggatgatcac aatgcctgca atctgcaaaa cggtcatatg ctcatgcaat
     7081 aaaagcattg aaacgatgac ggcgacgggc agttcgattg cactgagaat tgaagccatc
     7141 cccgtcccga cttttgggat gccgatggag aagagcagga tcggcagaat gatgccgaac
     7201 actcctaaaa tcaagccgta tatccataag ccatcaagca atgttccgtc ccaggcgatt
     7261 tgcggctcct ggaaaaaggt gatggcaact gcggcgaaga aagacatgaa cagtagtctc
     7321 gtgagcatat ccatcccttc cacttgccgc tgatttacca tgaggaatga agaaaaactg
     7381 actgccgcgg ccatccccca agcccatcct tgccaaggta taccgctcag atcggcatcg
     7441 atgacacctg ctgcaaaaat cgtgcctccg aataaaaaca ccagcgaaat gacttcaatt
     7501 cgcttcggca ggcgttgccg ggcaatgcaa tcgaagagca tgccgatcca tgtgaattga
     7561 aaaagcatga cgacagcgag ggaagcaggc attaaactga cagatttccc ataaactgtt
     7621 cctgtgatcg ctgttagcag acccgcgagc actaatgtat agccaccgcc gaacttcgga
     7681 agcgtccggc gggtgaaaag gtaaatgaga gcggccaata aaaagccggt atagtattgg
     7741 cttgtcactg cctgaggtgc tgtatacccg tctgcaatgg ccaatttaat gatcgtagaa
     7801 agaattccat agctgcacgc cccaagaatg acgaacagcg gatacatcca tggtttcatc
     7861 tgacgtcctc cagtccacgc aatagtataa catggacagg aaattacagg gtgaacagag
     7921 gcggttcaat ttgaattcaa ataatccaat acatcgctgg ccgctttttt tccttggaga
     7981 acgcagtcag ggaccccgac tccttcatac ccggccccgc aaagaaagac gcctggactt
     8041 gtccggtgca gttcctgtct gacggctgca agctcctcga catgatttac agggtatt
//
LOCUS       DASULI010000368         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1159001,
            whole genome shotgun sequence.
ACCESSION   DASULI010000368 DASULI010000000
VERSION     DASULI010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159001"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VFX34_07895"
     CDS             <1..152
                     /locus_tag="VFX34_07895"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5564867.1"
                     /translation="VVDFVDVLIPVINYEFPIFNVADAALTVGVVLIILHVIIDEKKN
                     KKKVA"
     gene            152..1057
                     /locus_tag="VFX34_07900"
     CDS             152..1057
                     /locus_tag="VFX34_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5564868.1"
                     /translation="MEPFEIEITEEHEKGRIDKVLSDINADWSRTQIQQWVKDGAVLV
                     NDGPVKQNYKVKTGDTITVMEPEPVELDIVAEDLDLEIVYEDADVLVVNKPRGMVVHP
                     APGHVTGTLVNGLMHQVKDLSGINGVMRPGIVHRIDKDTSGLLMVAKNDQSHVSLVDQ
                     LVDKSVTRVYTALVHGHIPHDQGTIDAPIGRDPKDRQSMTVIDKGKHAVTHFNVLERF
                     GDFTLVECRLETGRTHQIRVHMKYIGYPLAGDPKYGPKKTIDFNGQALHAGTIGFKHP
                     RTGEYLEFTQPLPDDFHELIEKMRS"
     gene            1264..1806
                     /gene="pyrR"
                     /locus_tag="VFX34_07905"
     CDS             1264..1806
                     /gene="pyrR"
                     /locus_tag="VFX34_07905"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX5564869.1"
                     /translation="MTEKAIILDEQAISRAVTRIAYEIIEKNKGIDECILVGIKTRGA
                     VLAKRLSDRIKQIEGKPILTGELDITLYRDDLQLKHENKEPLVHQVDIKHDVTEKKVI
                     LVDDVLYTGRTVRAAMDAVIDLGRPAAIQLAVLVDRGHRELPIRPDYVGKNIPTSNEE
                     RVVVNVIEEDGSDGVTIHKK"
     gene            1841..3133
                     /locus_tag="VFX34_07910"
     CDS             1841..3133
                     /locus_tag="VFX34_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEX5564870.1"
                     /translation="MNDNKVLDVLDKPAPGRWLALSLQHMFAMFGATILVPQFVGLSP
                     AIALLTSGLATLIFVLVTQFKVPAYLGSSFAFIAPIQVATSTGGIGSAMIGSMFVALV
                     YALVSLMIWTTGSGWIMKFLPPIVVGPVIMVIGLALAPTAVSMASTIQVNGEPVYNLL
                     HFSAAIVTLAAAIICLMFFRGVVSLMPVLIGIAVGYIYSAAIGILDFTKVHEAEWLEI
                     PKFLIPGIDYDFVVTPELLAIMVPISIVTISEHIGHQLVLGRVVDKDFIRDPGLNRSL
                     LGDGLGTLLSGLLGGPPKTTYGENIGVMAITRVYSVYVIIGAAVFAILFSFIGKLMAI
                     IATIPQAVLGGISILLFGIIASSGLRMLVDHKVDFGQQRNLVIASVILVIGIGGASIH
                     VSETFQVGGMALAAIVGVLLNLILPGRTNDSMADSPTE"
     gene            3285..>3956
                     /locus_tag="VFX34_07915"
     CDS             3285..>3956
                     /locus_tag="VFX34_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX5564871.1"
                     /translation="MKNLVSMKDLTIDEITTILDRAEILKQYGIRELPDKYMVSNLFF
                     EPSTRTKMSFEVAERKLGLETIPFEAGSSSALKGESLYDTLRTLEEIGMDVVVIRHPE
                     ERYYEDLIGKLNLAIINGGDGSGQHPTQSLLDLFTIKEAFGGFKGLNVLIAGDIAHSR
                     VAKSNADALTMLGANVTFLCPSEWAGEFPSVCEWEGIIEESDVVMLLRVQHERHGGES
                     RFTKEE"
BASE COUNT         1119 a          901 c          993 g          943 t
ORIGIN      
        1 aagtcgttga tttcgtggat gtcctaattc cggtcatcaa ttatgaattc ccgattttca
       61 atgtggcgga tgccgcattg acggtgggtg tcgtcctcat tatccttcac gtcattatcg
      121 atgaaaaaaa gaacaagaaa aaggtggcat aatggaaccg ttcgaaattg aaataacaga
      181 agaacatgaa aaaggcagaa tcgataaagt cctatcggat atcaatgccg actggtcacg
      241 aactcaaatt caacaatggg tgaaggacgg agcagttctc gtaaacgatg gacccgtaaa
      301 acagaactac aaagtgaaaa ccggagatac catcacggtg atggagcctg aacccgttga
      361 actcgacatc gtggcggaag atctcgatct ggaaatcgtc tatgaagacg cggatgtgct
      421 cgtcgtgaat aaaccacgtg gaatggtcgt ccaccctgcg cctgggcatg tcacaggaac
      481 gctcgtgaat ggcttgatgc accaagtgaa agacctctcc ggaattaacg gcgtcatgcg
      541 tcccggaatt gtccatcgaa tcgacaaaga cacttcaggt ttgctgatgg tcgctaaaaa
      601 tgatcagtca catgtatccc ttgtcgatca gctcgtcgat aaatcggtga cgcgcgttta
      661 cacggcgctc gtccatggcc atatccccca cgatcaaggc acgattgacg ccccgatcgg
      721 tagggacccg aaagaccgcc aaagcatgac ggttattgac aaagggaagc atgctgtcac
      781 ccacttcaac gtgttggaac gatttggcga ctttacgttg gtggaatgtc gactggaaac
      841 cggaaggact catcaaatcc gtgtgcacat gaagtatatc ggctacccgc tagcaggcga
      901 tccgaaatac ggaccgaaga agacaatcga cttcaacggg caggcattgc atgccggcac
      961 aatcggcttt aaacacccgc gcacggggga atatttggag ttcacacaac ctttgccgga
     1021 tgacttccat gaattgattg aaaaaatgcg ttcataaaat cgttgacaaa gtgaaaacgg
     1081 gcaactatac taatcaatga aatgaatagc gataccttta agaacagtcc agagaggctg
     1141 ggaaggttgc acggaagtac tgtaggatag ctgcgcgcta tgcatcagta cgcttctttc
     1201 tgcacctata cccttctgcc atcctggcag gaggtttttt tattatcaaa aggaggaatt
     1261 tccatgacgg aaaaagcgat cattcttgac gaacaggcaa tttcacgggc ggtgacaaga
     1321 atcgcctatg aaattatcga aaagaacaaa ggaattgacg aatgtattct tgtcggtatc
     1381 aaaacgagag gggctgtgct tgcgaaacgt ctctcggaca gaatcaagca aattgaagga
     1441 aaaccgatat tgactggcga gttggatatt acattgtatc gggatgacct ccaactcaag
     1501 catgagaata aggaacctct tgtccaccaa gtcgatataa aacacgatgt aacagaaaag
     1561 aaagtgatac ttgttgatga cgtcctttac acaggaagaa cagtgagagc tgcaatggat
     1621 gcagtcattg accttggaag accggcagcg atacagctag ctgttctcgt tgacaggggc
     1681 catcgtgaat tgccgatccg ccccgattac gtcggtaaaa acattccgac atcgaatgaa
     1741 gagcgagtag tcgtcaacgt catcgaagaa gacggatcag acggcgtgac catccataaa
     1801 aaataaaaac taactagata gggattgaga gagcgaaatg atgaatgaca acaaagtgtt
     1861 agatgtactc gataaaccag ctccgggaag atggcttgcc cttagtttgc agcatatgtt
     1921 tgcaatgttc ggagcgacga tcctcgtgcc gcaattcgtc ggtttaagcc cggccatcgc
     1981 tttattgacg agcgggcttg cgacgctcat cttcgttcta gtcactcaat tcaaggttcc
     2041 cgcctactta ggttcgtcgt tcgctttcat tgcgccgatc caagtggcga cgagcacagg
     2101 cggcattgga agtgcgatga tcggcagcat gtttgtcgca ctggtctatg cactcgtatc
     2161 gttgatgatt tggacgacgg gctccggttg gatcatgaaa ttccttccgc caatcgttgt
     2221 cggcccggtc atcatggtta tcggattagc attggcacct actgcagtca gcatggccag
     2281 cacaatccag gtgaacggtg aaccagtgta caatcttctg catttctcgg ccgcaatcgt
     2341 cacattggca gcggcgatca tctgcctcat gttcttcaga ggagtcgtca gcctcatgcc
     2401 tgtactgatc ggaatcgcag taggttacat ctactcggca gcaatcggga tattggattt
     2461 cacaaaagtg catgaagccg aatggcttga aataccaaaa ttcctcatcc ccggaatcga
     2521 ttatgacttt gtcgttaccc ctgagctatt ggctatcatg gtaccgatct caatcgtgac
     2581 gatttcggag catatcgggc accagctcgt actcggccga gtcgttgata aagatttcat
     2641 tagagacccc ggtttgaaca ggtcgctatt aggggacggc cttggaacac tccttagcgg
     2701 actccttggc ggcccgccga aaacgactta cggtgaaaat atcggcgtca tggcaatcac
     2761 acgtgtctat agcgtctacg tcattatcgg agcggcagtg ttcgcaatat tgttctcctt
     2821 catcggcaaa ctgatggcga ttatcgctac catcccccaa gcggtgcttg gcggaatctc
     2881 catattgctg ttcggtatca tcgcatcctc tggattgagg atgctagtcg accacaaagt
     2941 cgatttcggg cagcaacgga atctagtcat cgcctcagtc attctggtca tcggaatcgg
     3001 cggtgcctca atccatgtaa gtgaaacgtt ccaagtaggc ggaatggcgc ttgcagcaat
     3061 cgtcggtgtt cttcttaacc tgatcttgcc gggcagaacg aatgatagca tggcagatag
     3121 tccaacagaa tagaaagacc ttttaacatt gtacagagag acaaggaaag gttcggttga
     3181 cagacggtcg ttcaaaatga atgactattg ttcagtcatg cccaactggc ctttccatga
     3241 actggaaagg ccatttttta tccaaacgga aggggatttt caaaatgaaa aacctagtat
     3301 caatgaaaga cttaacaatc gatgaaatta caaccatttt agaccgggct gaaattctca
     3361 agcagtatgg aatccgtgaa ttacctgata aatatatggt tagcaatctt ttcttcgaac
     3421 cgagtacacg cacaaaaatg agtttcgaag tggcggaacg gaaattaggt cttgaaacga
     3481 tcccgtttga agcaggctct tcaagcgcgc ttaaaggaga aagtctgtac gacacgcttc
     3541 gcacgctaga ggaaatcggc atggatgtcg tcgtcatcag gcacccggaa gaacgctatt
     3601 atgaagacct gatcggaaaa ttgaatttag caatcatcaa tggcggcgat ggatcggggc
     3661 agcatccaac acaatcccta ctggatctct tcacgataaa agaagctttt ggcggtttca
     3721 aaggactgaa tgtcctgatt gctggagaca tcgcccatag cagggttgca aaatcgaatg
     3781 cggatgcttt aacgatgcta ggtgcaaatg taacattcct ttgtccttcc gagtgggcgg
     3841 gcgaattccc atccgtctgt gagtgggaag gaatcatcga agagagtgat gtcgtcatgc
     3901 tgctaagagt ccagcatgag agacacggcg gcgaatcaag gtttacgaaa gaagaa
//
LOCUS       DASULI010000369         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_205540, whole
            genome shotgun sequence.
ACCESSION   DASULI010000369 DASULI010000000
VERSION     DASULI010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205540"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFX34_07920"
     CDS             complement(<1..536)
                     /locus_tag="VFX34_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5564872.1"
                     /translation="MSFLEVLYFIVPTSIAYAAPLILTAIGGVFSERSGVVNIGLEGL
                     MVMGAFIGIVFNLAYADVFGGWTPWIALLVAMLVSAIFSIMHAVASVSFRADQVVSGV
                     AINMLGIAIALFSVKMMFGKGQTDFIQKKIPRVNIPFLQDIPVLGPMFFKSVYGTSVL
                     AIAVAVIAWFIIYKTPFGL"
     gene            complement(538..1584)
                     /locus_tag="VFX34_07925"
     CDS             complement(538..1584)
                     /locus_tag="VFX34_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5564873.1"
                     /translation="MSNRVIKILVPAISIILGLLVGAVVMLFSGYDPVAGYIALWNGI
                     FGDSYAIGETIRQISPYILAGLAVAFAFRTGLFNIGVEGQLMVGWFASAYVGAAFELP
                     MYIHLPFALLAAALAGALWGLVPGILKATLRVHEVIVTIMMNYVALHVVNALIKTVSG
                     GSYKTEKIQPTASLRSEFLSNLTDYSTLHYGIFVALAMVVVMWFILEKTKTGFELKSV
                     GFNENASHYAGMSVNKNIILSMVISGAFAGLGGAMEGLGTFGSMTNQGGFTGIGFDGI
                     AVALLGANSPIGVIFGASLFGSLKYGANNMPNAANIPLEIVSIVIALIIFFVASGYII
                     RVGLQRLNKKKEAK"
     gene            complement(1577..3133)
                     /locus_tag="VFX34_07930"
     CDS             complement(1577..3133)
                     /locus_tag="VFX34_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564874.1"
                     /translation="MEYVIEMLNITKQFGKFRANDNITLQLGKGEIHALLGENGAGKT
                     TLMNVLFGLYQPEEGEIRVNGKKVNVTSPNVANDLGIGMVHQHFMLVENLTVTENIIL
                     GNEPKKMGVVNIKDAAKKVAEISNTYGLNVDPYAKIEDISVGMQQRVEILKTLYRGAN
                     ILIFDEPTASLTPQEIQELIGIMKKLITEGKSIILITHKLQEIMDVSDRVTVIRKGQG
                     IGTVVTSETNPEELATLMVGRQVTFKTEKGPANPQEEVLNIENLVVLDYRGVEKVKGL
                     NLSVRKGEIVGLAGIDGNGQSELIEAIAGLRKVKSGKITINSKDITGMKPRRITESGL
                     GHIPQDRHKHGLVLDFSVGYNAALQEYYHEPYSKNGLMHYDVISEKATKIIEEYDVRT
                     QGEHELARALSGGNQQKLIIGREVSRDPELLIAALPTRGLDVGAIEFIHKRLIEQRDK
                     GKAVFLISFELDEVMNVSDRIAVIHDGQIVDTVIPSETTEKELGLLMAGHSKDEKVQH
                     ERKEGADRDV"
     gene            complement(3300..>4178)
                     /locus_tag="VFX34_07935"
     CDS             complement(3300..>4178)
                     /locus_tag="VFX34_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEX5564875.1"
                     /translation="AWEGIQKFGKDNGLSEGDGGYAYLQSKGEADYTSNLNALTRRDF
                     DLVFGVGFMMEEAINTIAEQQPDTNFGIIDGVVDQPNVASILFKEQEGAYLAGVAAAL
                     MTKSNKIGFVGGMEIPVIERFHAGFVAGVEAVNPDIKVDVQYTGAFDKAELGKAAAGR
                     MYSSGVDIIFHAAGGTGNGVFTEAKERKAADKDAYVWVIGVDSDQYDEGQVGDDNITM
                     TSMLKRVDVAVQDISKLAQEGKFPGGETKVYGLEDDGVTLADSRGAIPQDVMDKVEEY
                     KKKIASGEIKVPEFVE"
BASE COUNT         1122 a         1072 c          825 g         1159 t
ORIGIN      
        1 aggccgaatg gagtcttata gatgatgaac catgcaatga ccgctactgc aattgccaat
       61 accgaagtgc cgtaaaccga tttgaaaaac atcggcccga gtacagggat atcctgtaaa
      121 aacgggatat tgacacgtgg aattttcttt tggatgaaat ctgtctgtcc tttaccgaac
      181 atcattttta cagagaacaa agcaatcgca atccctagca tgttgatcgc caccccggaa
      241 acgacctggt cggcccggaa tgaaaccgat gcaacagcat gcatgatcga aaagatggcg
      301 gaaacaagca tggctaccaa taatgcaatc caaggcgtcc aaccgccgaa gacatcggca
      361 tatgccaagt tgaagacaat cccaatgaac gcgcccatga ccataaggcc ttcaagtccg
      421 atattgacga caccggaacg ttcagagaaa acacctccga tggcagtgag aatgagaggg
      481 gcggcataag cgattgaagt tggcacgata aaatatagca cttctaaaaa gctcatgtca
      541 tttcgcctcc tttttcttat tcaaccgttg cagtcccacc cggatgatat acccggaggc
      601 aacaaagaat atgataagtg ctatgacgat cgatacgatt tcaagcggga tatttgccgc
      661 attcggcata ttattcgctc cgtatttcaa tgagccgaat agtgatgccc cgaagatgac
      721 gccaataggt gagttggcac caagcaaggc gacggcaatc ccgtcaaagc caataccggt
      781 gaatcctcct tggttcgtca tgcttccaaa agtgccaaga ccctccatgg caccgccaag
      841 ccctgcgaat gcacccgaaa tgaccatgga taaaatgatg tttttattta cgctcatccc
      901 ggcataatgg gatgcgttct cgttaaagcc taccgacttc aactcaaaac cggttttcgt
      961 cttttcaagg atgaaccaca tgacgacaac cattgcaagc gcaacgaaaa ttccatagtg
     1021 aagagtggaa taatcagtca gatttgaaag gaattccgag cgcagcgacg cagtcggctg
     1081 tatcttttcc gtcttgtagc tgccgccgga tactgtttta atcaaagcat taacgacatg
     1141 aagggcaacg taattcatca tgatcgtgac aataacttcg tggacacgta aagtcgcttt
     1201 caaaatacca ggcaccaagc cccaaagcgc ccccgccaaa gcggcagcca atagagcgaa
     1261 cgggagatgg atgtacatcg gcaattcaaa tgcagcaccg acataagcgg aagcaaacca
     1321 tccgaccatg agctgccctt cgactccgat gttgaaaagc cctgtacgga atgcaaatgc
     1381 aacggccagc cccgcaagga tatacgggct gatctgccgg atcgtttcac cgattgcata
     1441 gctatctcca aaaataccat tccataatgc tatatagccg gccacgggat cgtagccgct
     1501 gaataacatg acgacggctc cgacgaggag cccaaggatg atcgaaatgg ctggcactaa
     1561 aattttgatt actctattag acatctcgat ccgcaccttc tttcctttca tgttgcactt
     1621 tctcatcctt tgaatgccca gccatcaata gaccgagttc cttttcagtc gtttccgacg
     1681 ggatgactgt atcaacgatc tgtccgtcat ggatgaccgc aatccgatcg gatacgttca
     1741 tgacttcatc aagttcaaag gaaatgagga agaccgcttt tcctttatct cgctgttcaa
     1801 taagcctttt atggatgaat tcaatcgctc cgacatccag ccccctcgtc ggcaatgccg
     1861 cgataagcag ttcgggatca cgtgatacct ctcgtccaat gatcagcttt tgctgatttc
     1921 ctccagatag agctcttgca agttcatgtt cgccttgagt cctgacgtca tattcttcaa
     1981 ttattttcgt agctttttcc gaaatgacat cataatgcat gaggccattt tttgaatacg
     2041 gttcatggta atattcctgc aaagccgcat tgtaaccgac agaaaagtcg aggactagcc
     2101 catgcttatg acggtcttgc ggaatatgcc ccaatcctga ttccgtaatt cttctaggct
     2161 tcatccctgt aatatctttt gaattgatgg tgatcttacc gctcttaacc ttccgcagac
     2221 cggcaatcgc ttcaatcaat tcggattgcc catttccatc gattccggca agaccgacaa
     2281 tctcgccttt tcggacggaa agattcaagc ctttcacttt ttcaacgcct ctataatcca
     2341 agacaacaag attttcaatg ttcaatactt cttcctgcgg atttgcaggt cctttttccg
     2401 tcttgaaagt aacttgtcta cctaccatca atgttgcaag ctcttcaggg ttcgtctcag
     2461 atgttacaac tgtaccgatt ccctgtcctt ttcggatgac tgttactcga tcggacacat
     2521 ccatgatttc ttgcaattta tgagtgataa gaatgatcga ttttccttca gttatgagct
     2581 tcttcataat tccgatcaat tcctgtattt cctgaggtgt caaggaagca gtcggttcgt
     2641 cgaagattaa tatgttagca ccacgataaa gcgttttcag aatttccaca cgttgctgca
     2701 tcccgaccga aatgtcttcg atcttcgcgt aaggatcgac attcaaacca tacgtattgg
     2761 aaatctcggc cactttcttt gcagcatctt taatattgac gacacccatc tttttcggct
     2821 cattgcccaa aatgatgttt tcggtaactg tcaagttttc gacgagcatg aagtgttggt
     2881 gaaccattcc aatcccaaga tcatttgcaa cattcggact tgtcacatta acctttttcc
     2941 cgttgacgcg tatttcccct tcttcaggtt ggtaaagacc gaacaacaca ttcatcaatg
     3001 ttgtttttcc ggccccattc tcacctagaa gcgcgtggat ttcccctttc cccaattgca
     3061 aggtgatgtt gtcattcgcc cggaatttac cgaattgctt cgtaatgttc aacatctcaa
     3121 tcacatattc caatggattc acttccttgt ataatagttt ctctttttag ttttaataaa
     3181 atgaacacaa taaaaggttc tttaaataaa aaaacctttt actctatcat tttattttcc
     3241 gtattatggt catccaaaca acaaaggccg gactgaaccg gccctcgtcg tcatactact
     3301 tattcaacaa actcaggtac tttaatttca cctgaagcga ttttcttctt gtactcttcc
     3361 actttatcca tcacgtcttg tggaatcgca ccgcgggaat ccgccaatgt cacaccgtcg
     3421 tcttcaagtc cataaacttt tgtttcgccg ccagggaatt tgccttcctg agcaagcttt
     3481 gaaatatctt gaaccgctac gtcaacacgt ttaagcattg aagtcattgt gatattgtcg
     3541 tcaccgactt gaccttcatc atattgatcg gagtcaacac cgataaccca tacatatgca
     3601 tctttatcag ccgctttacg ctctttcgct tctgtgaaga caccgttacc agtaccacca
     3661 gctgcgtgga agatgatatc tacgccagat gaatacatac ggccagctgc agctttacct
     3721 aattcagctt tgtcgaaagc ccctgtgtat tgaacatcaa ctttgatgtc agggttcact
     3781 gcttcaacac ctgctacgaa tcctgcatgg aaacgctcga ttaccggaat ttccataccg
     3841 cctacgaaac cgattttatt tgattttgtc atcaatgctg cagcaactcc agcaaggtaa
     3901 gcaccttctt gctccttgaa caggatgctt gcgacgttcg gctgatcaac gacgccatcg
     3961 atgattccga aatttgtgtc aggttgttgc tcggcaattg tattgatagc ttcttccatc
     4021 atgaaaccta caccgaaaac caagtcgaag tcacgacggg taagcgcatt caaattggac
     4081 gtatagtccg cttcgccttt cgattgaagg tatgcgtaac ctccgtcccc ttcactcaat
     4141 ccgttgtctt taccgaattt ctggattcct tcccatgc
//
LOCUS       DASULI010000370         4497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1594100,
            whole genome shotgun sequence.
ACCESSION   DASULI010000370 DASULI010000000
VERSION     DASULI010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594100"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..443
                     /locus_tag="VFX34_07940"
     CDS             51..443
                     /locus_tag="VFX34_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribose kinase"
                     /protein_id="HEX5564876.1"
                     /translation="MLQQYFIELGEGYGDVYELLELMETNSQRLHRTFIFSSNTKSGQ
                     AISLAAAFRPAQDSNFMPIYICREGITQKDESKSKRRLLFEEASTSFGKEPIFIGLKH
                     SSEFADTKLFYQYITGILRLNHLVPPLQ"
     gene            complement(460..732)
                     /locus_tag="VFX34_07945"
     CDS             complement(460..732)
                     /locus_tag="VFX34_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEX5564877.1"
                     /translation="MIDRQGIIVYLHHLKQAKSLRKYGHVHYISRKMKYVVLYCDQVD
                     VDETIRKLERLPSVKRVLPSYRPFVKTEYENVKPDKAKEYDYKIGL"
     gene            868..1773
                     /locus_tag="VFX34_07950"
     CDS             868..1773
                     /locus_tag="VFX34_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5564878.1"
                     /translation="MGRKTKVALTVGAAGVAAWAASKVVAKPQPRPRKEALEFKDPVV
                     LSHRGGMKSAPEHTMAAFSISAELGVHGFAVDIRLTKDEEIVLFHDENADRTTDFSGR
                     ISDYTIDELKKADAGYMFEDAEGNFPFRGKDEKIVTLHELLMAYPHMLIAINLKDSPD
                     TYEGSLMPSKLWRLLEETGSEERVIVMSAFDEQTDRFNLYAQNKVATGAGNDEVKKAY
                     ASYSSKFGHLYNPRADLFCIPEKMGLFPMNTESFVSFLSNLNVSVYYNNVSDRDSFEK
                     LLNAGAAGFITDEPGLAMEVIQRNS"
     gene            complement(2062..2505)
                     /locus_tag="VFX34_07955"
     CDS             complement(2062..2505)
                     /locus_tag="VFX34_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEX5564879.1"
                     /translation="MMMTDEWMSIIEQSEILSDMLLSSEIIQEYRRANHAVYSDKSLA
                     EAIYDFTNMKERYEEVQRFGRYHPDYKTVMKDIRIQKRELDMNEQVAALRLAENDVQR
                     LLNEISAIIAGAVSDSIKVPAGDSFFVDSSCGGGCGTGGSCSCSA"
     gene            complement(2575..3930)
                     /locus_tag="VFX34_07960"
     CDS             complement(2575..3930)
                     /locus_tag="VFX34_07960"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX5564880.1"
                     /translation="MPISGITERSGNVREGFIFVARKGARDDGILHVQEAIELGAVAI
                     VIDRNENVDFDAIQEITLVTVPDGRKFISHASAKLAGNPSESMTIIAVTGTNGKTTVT
                     HFIGQLLTMLGEKAAVIGTTGVFMDGRKVILDVPEMTTLPAEFLHPLLKECMHAGMKY
                     IVMEASSLGLSTSRLDHCKIDIGVFLNIGVDHYEEHGSKEAYIAAKKRLTTMAMDLVV
                     NGDDRQCVQLIHDAHRPVHFFNELMVEGRVVPCDLYPTSLPGKHNRLNALAAVTVMQI
                     LGRDLFETLPLCDKLVLPEGRLQIIREGGVDVFIDYAHTPDALQTVLVSLMEESIGEL
                     ITVFGCGGNRDRGKRPQMGKIAATYSSKVFITSDNPRNEEPSSIIEEILAGISCEKEK
                     VVVEMDRRAAIEKAVRYAKAGDIILIAGKGHEKTQQIGHELLTFSDYEVARAALDDKY
                     K"
     gene            4133..>4497
                     /locus_tag="VFX34_07965"
     CDS             4133..>4497
                     /locus_tag="VFX34_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5564881.1"
                     /translation="MKDKQSRKICIGHIKSGLENHQNVVVVVSAIGRGDDPYSTDRLL
                     RLTDAFIYSKSASDLAASCGELIASAILSAELEEAGVPNRILHGIQAGIWTSGDFGDG
                     TISDVDPTMIVDSLQESGCV"
BASE COUNT         1242 a         1067 c          955 g         1233 t
ORIGIN      
        1 taaaaatgat aaaatgaact atatccgaat ggagaaaggt agtgtcatga atgttacaac
       61 aatattttat cgaattaggc gaagggtatg gagatgtcta tgagctgttg gaattgatgg
      121 aaacgaattc acaacgtctt catagaacct tcatcttctc ctccaacaca aaaagcggac
      181 aagcaatttc attggcagct gcattccgcc cagcccaaga cagcaatttc atgccgatat
      241 acatatgcag ggaaggcatt acacaaaaag atgaatccaa atcgaagaga cggcttctct
      301 ttgaggaagc ttctacttca tttggaaagg aacccatctt catcggactg aagcattctt
      361 ccgaatttgc cgatactaaa ttgttctatc agtatataac aggaatactg cgtcttaatc
      421 atcttgttcc gccattacaa taaaattggc ctttcatgat taaaggccta ttttataatc
      481 gtattccttc gccttatcag gtttgacatt ttcgtattcc gttttgacga atggccgata
      541 agatggcaat acccttttaa cggaaggaag tcgttcaagt tttcggattg tctcatcgac
      601 gtccacctga tcacaataaa ggacgacata tttcattttt cttgaaatgt aatggacatg
      661 gccatatttt cgtaatgatt ttgcctgttt caaatgatgg agataaacta ttatcccttg
      721 tcgatcaatc atgattttcc cctcttttcc taccccatac aataccatag tagagaaatg
      781 gcctgcaacc gaacattgct gacatcttcc gttttttccc ctataatgag gaaagacatg
      841 tttacgaata acgaggagga tatgaaaatg ggaagaaaaa caaaagtggc tttaacggta
      901 ggtgctgccg gcgtagctgc ctgggctgca tcgaaagtag tcgctaagcc ccaaccacgc
      961 cccagaaagg aagcacttga attcaaggat ccggtcgtcc tttctcatcg cggaggaatg
     1021 aaatccgctc cagagcatac gatggcagcg ttttcaattt cagcggagct cggggtccat
     1081 ggatttgccg tcgacatacg cttgacaaaa gatgaagaaa tcgtcctctt ccatgatgaa
     1141 aatgcagatc gaacaactga cttttccggc aggatttccg attatacgat agatgaattg
     1201 aagaaagcgg atgcgggcta tatgttcgag gatgcggaag ggaatttccc tttccgtggc
     1261 aaagacgaga aaatcgtcac tttacatgaa ctgttaatgg catatccgca tatgttgatt
     1321 gcgattaacc tgaaggactc gccggataca tacgaaggca gcctaatgcc ctctaagctt
     1381 tggcgtctat tggaggaaac gggatctgaa gaaagggtga ttgtcatgag cgcttttgat
     1441 gagcagacgg atcgcttcaa tctctacgct caaaataaag ttgcaacagg agccgggaat
     1501 gatgaagtga aaaaagcgta tgcgtcctac tcaagcaaat tcggacatct ttacaatccg
     1561 cgggcagacc tgttttgcat tcctgaaaaa atgggcctgt ttccgatgaa tacggaaagc
     1621 ttcgtgagct tcctatccaa tttgaacgtt tccgtctact acaataatgt cagtgatagg
     1681 gattcatttg aaaaactctt aaacgccgga gctgccggat tcattacgga cgagccggga
     1741 ctggccatgg aagtcatcca acggaattca taataacaga aaaaaaacgg ttgccaatca
     1801 ttgaatacag gcaaccgttt ttcatcatat tcccttatgc agaacaggag ccaatcgaac
     1861 agcgaagggt tcgatttgcc gtatttctgc gcactttgca gaaattaagg cagccaggca
     1921 ttaatctcct tacctagtca cgctgaacag gagcagcttc cacctgtgcc acagccaatc
     1981 gaacagcgaa gggttcgatt tgccgtattt ctgcgtactt tgcagaaatt aaggcagcca
     2041 ggtatcaatc tccttaccta gtcacgctga acaggagcag cttccacctg tgccacatcc
     2101 cccaccgcaa gatgaatcta caaagaaact atctcccgcg ggcactttga tggaatcgga
     2161 taccgctcct gcaatgatcg cgctgatttc attcaacagg cgttgtacat cattctctgc
     2221 aagacgcaag gcagcgactt gctcattcat atccagttcc cttttttgta tacgtatatc
     2281 tttcatgact gttttatagt caggatggta tctgccgaaa cgttgcactt cttcgtaacg
     2341 ctccttcata ttggtaaaat catagatcgc ttcagccaac gatttgtcgg aatagacggc
     2401 atgattcgct cttctgtact cttggatgat ttcggaagag agcaacatat cggagagtat
     2461 ttctgattgc tcaatgatag acatccattc atctgtcatc atcatagtgc tttccctcca
     2521 ttcccactaa tcataccaga aaaagcgttt cctttgcatg cattctgact agttttattt
     2581 atacttatcg tctaatgccg cccttgcgac ttcataatca gagaatgtca ggagttcatg
     2641 acctatctgc tgtgtctttt cgtgcccttt cccggcaatc aggatgatat cccctgcttt
     2701 ggcgtatcgg acagcttttt cgattgcggc ccttcgatcc atctctacaa ccactttttc
     2761 cttttcacag ctgattccgg ctaaaatctc ttctattatg gaagacggct cttcattcct
     2821 tgggttgtcg gatgtaataa atactttaga tgagtacgtc gcagcaatct ttcccatctg
     2881 tggccgcttt ccacggtcac gattgcctcc gcagccgaat acggtaatca attcacctat
     2941 ggattcttcc atcaatgaaa ctaaaactgt ctgaagcgca tcgggagtat gggcatagtc
     3001 gataaagaca tccacacccc cttcacgtat gatttgtagt cgcccctccg gcaggacgag
     3061 cttgtcacaa aggggcaaag tttcaaatag atcccgaccg agtatttgca taacagttac
     3121 ggcggcaagc gcatttaacc gattgtgctt ccccggcaaa gaggttggat ataagtcgca
     3181 aggcactact cttccttcaa ccatcagctc attgaaaaag tggactggtc tgtgtgcatc
     3241 atggataagc tgtacacatt gcctgtcatc cccattcacg acaaggtcca tcgccatcgt
     3301 tgtcagcctt tttttcgctg caatataggc ttccttgctg ccatgttctt cataatgatc
     3361 gacccctata ttaaggaata caccgatatc gattttgcaa tggtccaatc ttgatgtgga
     3421 taatccgagg gaagaggctt ccatcacgat atatttcatt ccagcgtgca tgcattcctt
     3481 taacagcgga tgaagaaatt ctgccggcaa agttgtcatt tccggcacat cgagaatcac
     3541 tttcctgccg tccatgaaaa ctccggtcgt cccaatgacc gctgcctttt ctccaagcat
     3601 tgtcaataat tgtccgatga aatgggtgac agtcgtcttg ccattcgttc ctgtcactgc
     3661 aatgattgtc attgattcgg aaggattccc tgcaagcttg gcactggcgt gcgatatgaa
     3721 ctttcttccg tccggcacag tgaccagcgt gatttcctgg atagcatcga aatccacgtt
     3781 ttcattgcga tctatgacga ttgcaacggc accgagttca atcgcttctt ggacatgcaa
     3841 aataccatcg tcacgagcac ctttcctcgc gacgaaaatg aatccttccc tcacatttcc
     3901 agacctctcc gtaatgccgc taatcggcac accgacacta cccgaaaccg tacagggcca
     3961 gtctttcaag atctcagata ataacagaag ccccctcccc ttcccgaaca attttcatga
     4021 atagcgaata tattgagagc gaaccatact ttatcgtatg aaggaaatgg aacgaacgac
     4081 acgaagaaag ttgggagctt gaatgatagt tcaaaaattt ggcggagtgg ccatgaaaga
     4141 caaacaatca agaaaaattt gcattggtca catcaaatct ggattggaaa accatcaaaa
     4201 tgtcgtcgta gtcgtttcag caattggaag aggagatgat ccctactcca cggatcggtt
     4261 attacgtcta actgacgcat tcatctattc taaatccgca agtgaccttg ctgcctcatg
     4321 cggcgagctt atcgcatcgg ccatcctatc agccgaattg gaagaggcag gcgttccgaa
     4381 caggatacta catggaattc aagcaggtat ttggacatcc ggggatttcg gtgatgggac
     4441 gatcagtgat gtcgatccta caatgatcgt cgatagttta caggaatccg gctgcgt
//
LOCUS       DASULI010000371         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_326675, whole
            genome shotgun sequence.
ACCESSION   DASULI010000371 DASULI010000000
VERSION     DASULI010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326675"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFX34_07970"
     CDS             complement(<1..606)
                     /locus_tag="VFX34_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UV damage repair protein UvrX"
                     /protein_id="HEX5564882.1"
                     /translation="MYEGLPDRKVVCLDMRSFYASCAAAIEGIDVMDTPIAIIGNKER
                     KGGIVLAASPALKKEFGVQTGMRLYEIPDDPSIRLIEPKMQFYIDVSMEIAKHLNRYV
                     PKEAIHVYSVDESFIDLGGTERLWGDPEDTVKRIQDELVSQFQLRSACGMGPNMLMAK
                     LALDLDAKKTGFARWTYEDVPKRLWPVAPLSRMWGIGSRLER"
     gene            complement(790..1590)
                     /locus_tag="VFX34_07975"
     CDS             complement(790..1590)
                     /locus_tag="VFX34_07975"
                     /inference="COORDINATES: protein motif:HMM:NF042666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6612 family protein"
                     /protein_id="HEX5564883.1"
                     /translation="MKKIISALAAGTLVLLLAACSSSGVAQKKMTAGDLLDKSQKAMD
                     SSLKAVRSHIVFDDYAVTVYDGDIENPEKASVKFDMNVDAFLDPAKVHLQANVLPLGV
                     KAWNMDLYQVGDRVFANDDRKEGWDRLPSGSVEELFGTLVAETYPVLDLSKFKEFEDE
                     FILEPIEYGYALKLTLDRDGFKRLKELFPDVGPREDGFSIIDKMELAIIFNNKTSYVT
                     SFKMSADIRSFRDGNSYRSRQKLNATYSYFNDIEDFKLPKEVSDLATD"
     gene            complement(1736..>2670)
                     /gene="menC"
                     /locus_tag="VFX34_07980"
     CDS             complement(1736..>2670)
                     /gene="menC"
                     /locus_tag="VFX34_07980"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX5564884.1"
                     /translation="TVETNLHMIRDFLIPLLLGKEIGHPDRVSNLFAPIRGNNMAKAA
                     LECAVWDLYAKRKGIPLAEALGGVRERIEVGISIGIQKDMADLITNVQRYLKEGYKRI
                     KVKIKPGAEMDILRGLRDAFPHVPLMADANSAYSLDDIDLLKQLDEFDLMMIEQPLAH
                     DDIIDHSVLQKELKTPICLDESIHSLEDVRKAVELGSTKVINVKIGRVGGLSEAKRIH
                     DYCMEKGVPVWCGGMLEAGIGRAHNIALTSLPNFILPGDTAGSSRYWEKDIIEPEVVV
                     EDGYITVPTDPGISFTPVLSSIEEFTTDIWKFRA"
BASE COUNT          676 a          708 c          562 g          724 t
ORIGIN      
        1 cctttcaagc cggctgccga tgccccacat ccggctgagc ggggcgaccg gccataacct
       61 ctttggtacg tcttcatacg tccaacgggc aaagccggtc tttttcgcat ccaagtcaag
      121 ggcgagcttc gccatgagca tattcggccc cattccgcag gccgacctta gctgaaattg
      181 gctgacaagc tcgtcttgaa tccttttgac cgtatcttca gggtcacccc ataaccgctc
      241 tgtgcctcct aagtcgatga agctttcgtc gacggaatat acatgaatcg cctctttcgg
      301 cacgtagcgg ttcaaatgct tggcaatttc catcgagaca tcgatataaa actgcatttt
      361 cggttcgata agccgtatgg aggggtcatc gggaatttca tatagccgca ttccggtctg
      421 cacgccgaat tccttcttca aggcgggtga agccgccagg acgatgcctc ctttccgttc
      481 tttattgccg atgatggcga taggcgtatc catgacatca attccctcga ttgcagccgc
      541 acaactagcg tagaaactcc gcatatcgag gcatacgact ttccgatccg gcaaaccttc
      601 gtacatgtgc atccctccga aaagaacact tgttctaaat agtatacccg gaacagatgt
      661 tcatattcaa tggttcttaa tgccaattgg gaaaaatgga aagcgagctg ctacaatgta
      721 gtctcttagt cctgtgttct acaaatgaaa aagcatccat tcctgttcaa ggagatagat
      781 gcttttcatt taatccgttg ccaaatcact gacttccttc ggcaacttga aatcttcgat
      841 atcattgaaa taactgtatg tggcgttcag tttctgacgg gatcgatagg agttcccatc
      901 tctgaagctc cttatatccg cagacatttt gaaactagtc acataagaag ttttgttatt
      961 gaagataatg gccaattcca ttttatcgat gatgctgaat ccgtcctctc gtgggccaac
     1021 gtccgggaag agttccttca gccgtttaaa tccatcgcgg tccaacgtca atttcaatgc
     1081 atatccatat tcaatcggct ccaggataaa ttcgtcttcg aactctttga acttagataa
     1141 gtcaagaacc ggatatgttt cggccactaa tgtgccaaac agttcttcaa ctgaaccgga
     1201 aggcaatcga tcccagcctt cttttcggtc gtcatttgca aatacccgat ctcccacttg
     1261 atacaagtcc atattccatg ccttcacgcc aagcggaagg acattggcct gcaaatgaac
     1321 tttcgcaggg tcaagaaaag catcgacatt catatcgaat ttcacgctgg ctttctccgg
     1381 attttcaata tcgccatcat agacggtcac cgcgtaatca tcaaatacaa tatgtgagcg
     1441 gacggctttc aatgacgagt ccatcgcctt ctgggattta tccaataaat ctccggctgt
     1501 cattttcttt tgagccacac ccgaactgct gcaagcagct aacaaaagaa caagcgtccc
     1561 ggcagcaagg gcactaatta tttttttcat atgtaaactc cctctctaca aaacctacaa
     1621 tcattttact ggaatacccg cacttcatca atccattgga cggatattgc gattgggaag
     1681 ttacatccgt cgcgaacagc ggaagagatc tcgttcccgc cggtgaagtt ctcatttaag
     1741 ctcgaaactt ccagatgtct gtcgtgaatt cctcaatgga actgagaaca ggagtaaagc
     1801 tgattcccgg gtcagtcggc acagtaatat agccatcttc cacaaccact tcaggttcaa
     1861 taatatcctt ttcccaataa cgggacgagc ccgccgtatc tcctggaagg atgaagttcg
     1921 gaagtgaggt caatgcaata ttatgggcgc gcccaattcc cgcctccagc atgccgccgc
     1981 accagacggg aacacctttc tccatgcagt aatcatggat ccgcttcgct tctgacaagc
     2041 cgccgactct cccgattttc acattgatga cttttgtgct tcccagttcg accgctttcc
     2101 gcacatcttc caatgaatgg atgctttcat caaggcagat cggcgtcttc aactctttct
     2161 gcaaaactga gtgatcaata atatcgtcat gtgcaagcgg ttgctcgatc atcataaggt
     2221 cgaattcatc caactgtttt aacaggtcaa tatcatccaa ggaataagcc gaattggcat
     2281 ccgccattag cggcacgtgg gggaaagcat ccctcaatcc gcggaggata tccatttccg
     2341 ctccaggttt gattttcact ttgatgcgct tatagccttc cttcaaataa cgttgcacgt
     2401 tagtaatcaa gtcggccata tccttttgta ttccaatgct gataccgact tcgatacgct
     2461 cgcgcacgcc gcctaatgct tcggccaatg ggattccttt ccgttttgca taaagatccc
     2521 agacggcgca ctcaagggcg gcctttgcca tattgtttcc acggataggc gcgaacaggt
     2581 tgctgacccg atcaggatgc ccgatttctt tcccaaggag cagtgggatg aggaaatcgc
     2641 ggatcatatg caaatttgtt tccaccgttt
//
LOCUS       DASULI010000372         6455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_69263, whole
            genome shotgun sequence.
ACCESSION   DASULI010000372 DASULI010000000
VERSION     DASULI010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69263"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFX34_07985"
     CDS             complement(<1..896)
                     /locus_tag="VFX34_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5564885.1"
                     /translation="MTQMPMGAVPKKEKEKLKVIGKSKKSPKKVFYEMKRNYSAYLFL
                     LPKLILFTLFVAIPVVWAFILSFQKYKIFGSEFVGFANYIKVFESDAFRIALKNTMLF
                     TVVTVPFSVISALVLSMLIFELGKLSQSLFRSAFYLPTVTSMVIIAMVWRWMYNYDFG
                     LFNYVLGWFGVGPVNWLGQSSTALWALIIMQCLIPFGVGIIMYLASMGSISQSLYEAA
                     RMDGASKFKQWIHITIPLLKPTTLYLVLLSTIGSFQVFTQIIMMTGGGPGKSTETLVH
                     LIYKTGFRDFEFGLAGAQSVVL"
     gene            complement(981..2306)
                     /locus_tag="VFX34_07990"
     CDS             complement(981..2306)
                     /locus_tag="VFX34_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5564886.1"
                     /translation="MKKVWKKSALAALTLGFAATMLAACGDKEDAGNAEEFKGKITIW
                     DGPRWPDADDNKFHWLEDKIKEYEEAHEGIEIELVQVPWAEMGDKLGVAIAGKAWPDI
                     APVDISGSAVSIDHIEQGVIESLDPYFDKEAKKDFYDNALEAYTYDGKLYGIPNSITL
                     HTMLLNLDLFEEAGVEPPKDGKWTYEEFLEAAKKLTFDRDGDGKTDVYGFSTYIMPGY
                     YEAWPFFYKNGGSPLNEDMTEFTFDSPEAVKAIQDLADLKLKHNAAPETLGGNDVGGT
                     FQAWANEEQRTVAMEPWATWAIGAAQGKYPTNFMVAEYPSGDKDAATIGGVGGWVMMH
                     QEDVNKKAAVADFMKFISTAEEQYHMAQNYGIFPARISASEMDPFKDNPEMTRAMEMS
                     DQVVMLPRHPEWRKIDEAIQNELQLVFNGEKTAEQAMADAKKAVDQILE"
     gene            complement(2536..3693)
                     /locus_tag="VFX34_07995"
     CDS             complement(2536..3693)
                     /locus_tag="VFX34_07995"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX5564887.1"
                     /translation="MRVCGLMSGTSLDGLDIAVVDFTRNEGKIRHELLHFITMPYDDG
                     LKQELHKLMDPTAPLHLVSSMNMYLGERYADSLQKALQEAGIGLETVDLISSHGQTIW
                     HEPAVEPDRSYARPNTMQIGDIAVIAERTGKTVIGDFRTRDMAVGGQGAPLVPFADQV
                     LFQSESVGRILLNIGGIANMTVLPRLGSEQPIIAYDTGPGNMVIDACVARHSGGQSSF
                     DKDGELAKNGTIDEDWIDRLMDHKYFDAPAPKSTGRELFGAAFVETFWAEGDEHGLSS
                     TDKIATATMLTAKTIAAEIQRYIQSHDVKEVIISGGGVHNQTLMGFLKASLPEDLKFA
                     TTGDLGIDADAKEAFVFALLGYLGIHKLPNSLPAATGAEKATIMGKVAWGG"
     gene            complement(3813..5375)
                     /gene="nagZ"
                     /locus_tag="VFX34_08000"
     CDS             complement(3813..5375)
                     /gene="nagZ"
                     /locus_tag="VFX34_08000"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX5564888.1"
                     /translation="MKLQKKVGRLLIAGFKGITMSEEIKHLIHTYHIGGIILFGRNIG
                     TPEEILALTNSLQAEAKKAGYETPLLICIDQENGVVRRLGEGTTIIPGAMLLGATQDP
                     ANARKAGLMTGKELKALGINWNLAPVVDVNNNPKNPVIGVRSFGENPAAVAEMAMNSM
                     LGMQEAGVMTTLKHFPGHGDTSVDSHLDLPVIDHNLDRLHAVELVPFKECMAAGADAV
                     MSAHVYFPALEKEENVPATLSHSVITGLLREELGFDGVITTDCMEMDAIAKRIGTVEG
                     CVQAVEAGVDFVMVSHLHDLQEQAVIRLAEAVETGRISERRIEESIERIEKLARKYTS
                     WDEIEANQTVAEFVGSGKHHDEMKEIYVQGITEVKGPGIPIAAEERVLLIHPKNEYAM
                     MVEDKRYATLTMAEQLKKVHGNVVSMEIDGEGPGDMDMTELAARYNHIVMLTLNADKH
                     ENQRRIVEGLMASGKPVDVIAVRSPYDAAYFPKANRIICTYEFTQSAFEVVAEHLAGK
                     TAINGRLPVSID"
     gene            complement(5403..6110)
                     /locus_tag="VFX34_08005"
     CDS             complement(5403..6110)
                     /locus_tag="VFX34_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX5564889.1"
                     /translation="MQHVMIVGAHCGDGEIQAGAIAHKYAQAGYRVTFLHLTAGEKGA
                     PPHMHVEEYRRQKIREAEAAAAILGGTSITLAHRDAELTFNDAIVKEVATIFRREKPE
                     FVITHWENSMHPDHALCQKIVQDAWLKASLPGFDLEGLPPHGLRRVFHSENWEDMDGY
                     EPDIYIDVTDSFDAYLKALSCYWFIMNSTSFRYYDYYKALGTMRGCLARTTYAQTLKN
                     SRGLHVRKEGHIPGFPI"
     gene            complement(6095..>6455)
                     /locus_tag="VFX34_08010"
     CDS             complement(6095..>6455)
                     /locus_tag="VFX34_08010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5564890.1"
                     /translation="EMIENDGVHDGKKILDPHWLALSRRNFTPFADESRGLGWQLKGN
                     GASPAGDLMSKSTYGHTGFTGTSFYIDPEKELTVMLLTNRVYFGRHLAMLRLRPRLHN
                     IIYTSMFSQGDQVCSMS"
BASE COUNT         1493 a         1892 c         1375 g         1695 t
ORIGIN      
        1 aataatacga cggattgagc ccctgccaaa ccgaattcaa aatcccggaa gccagttttg
       61 taaatgaggt ggacaagcgt ctctgtggac ttgcccggtc cccctcctgt catcatgatg
      121 atctgcgtaa acacttggaa agaaccgatc gtgctcagca acactaaata caacgtcgta
      181 ggtttcaata aagggatcgt aatatggatc cattgcttga atttgcttgc cccatccatc
      241 cgtgcggctt catacaacga ttgggagatc gaccccatcg aagccaaata catgatgatc
      301 ccgacgccga acggaatgag gcattgcatg atgatcaacg cccataacgc cgtgctcgac
      361 tgtccgagcc agttgacagg cccgacgccg aaccagccca acacatagtt gaacagcccg
      421 aaatcgtaat tgtacatcca ccgccatacc atcgcgataa tgaccatcga cgtgactgtc
      481 ggcaaataga acgccgacct gaataggctt tgggacagtt tccccaattc gaaaataagc
      541 atggacagca caagggcact gatgacgcta aacggaaccg ttacgaccgt gaaaagcatc
      601 gtgttcttca atgcaatacg gaatgcatcg ctctcgaaca cttttatgta gttcgcgaac
      661 ccgacaaatt cactgccgaa gatcttgtat ttctgaaacg ataggatgaa tgcccacaca
      721 acggggattg caacgaaaag cgtaaaaaga atgagcttcg gcagcaaaaa gagatatgcg
      781 ctatagttcc gtttcatctc atagaacact ttcttaggac tcttcttcga ttttccgatt
      841 actttcaact tttctttttc ctttttcggc actgcaccca taggcatctg agtcatgttg
      901 acacccccta aaagtgaagc aggcgtacgc gatgcatagc ctgctttcat tctacttccc
      961 acattattcg tcttcgttta ttactccaat atttggtcta ccgctttttt agcatccgcc
     1021 atggcctgct cggctgtttt ttcaccgttg aacacgagct gaagctcatt ttggatcgcc
     1081 tcgtcgattt ttctccattc cggatgacgt ggaagcatga cgacttggtc ggacatttcc
     1141 atcgcacgtg tcatttccgg attgtctttg aacggatcca tttcagacgc actgatgcgc
     1201 gccgggaaga taccgtaatt ttgcgccata tgatactgct cttcagccgt ggaaatgaat
     1261 ttcatgaaat cggcgaccgc cgctttcttg ttcacatctt cctggtgcat catgacccat
     1321 ccgccgacgc cgccgattgt cgctgcatct ttatcgcctg aaggatattc tgcaaccatg
     1381 aagttcgtcg gatatttgcc ttgcgcagcg ccgatcgccc atgttgccca tggctccatt
     1441 gcgactgtac gctgttcttc gttcgcccat gcttggaatg ttccgccgac atcgttgccg
     1501 ccaagcgttt caggagcggc attatgcttc aatttcaagt cagccagatc ctggatcgcc
     1561 ttgaccgctt caggggaatc gaaggtaaac tccgtcatgt cctcgttaag cggcgatccg
     1621 ccatttttat agaagaacgg ccatgcttca tagtagccag gcatgatgta tgtcgagaat
     1681 ccgtatacat ccgtcttgcc atcgccatcc cggtcgaacg tcaatttttt cgctgcctct
     1741 aaaaactcct catacgtcca tttgccatct ttcggcggct cgacgccagc ctcttcaaac
     1801 agatccaagt tcagcagcat cgtgtgcaac gtaatactgt tcggaatccc gtacagcttt
     1861 ccgtcatacg tatacgcttc aagcgcattg tcgtagaaat ccttttttgc ctctttatcg
     1921 aaatacggat ccaatgattc gatgacgcct tgctcgatat gatcgatgct caccgcgctg
     1981 ccgctaatat cgacaggagc gatgtccggc caagctttcc ccgcgatggc gactccgagc
     2041 ttatctccca tttccgccca tggcacttgc accaattcga tttcaatgcc ttcatgcgct
     2101 tcttcgtatt ccttgatctt atcctccagc cagtggaact tattatcatc cgcgtccggc
     2161 catcttggtc catcccaaat ggtgatcttc cctttgaact cttcagcatt tcccgcatct
     2221 tccttgtcgc cgcaagctgc aagcatcgtc gctgcaaacc ccaacgtcaa ggccgcaaga
     2281 gcggatttct tccaaacttt cttcattttt tgaccccctg tttgtttttc tcgtgaattt
     2341 cccattattt attcccggca ggtaaaacgg ccttgctcaa atgcgcataa atcctaaaac
     2401 ttacgcataa acgccgtcat tcacgcataa atcgtgaaac tcacgcataa acgtcgccaa
     2461 acgcgcataa atcgtgaaac tcacgcataa acgccgccaa acgcgcatat atcccgaaac
     2521 tcacgcataa atgcctcaac cgccccaagc gaccttcccc ataatcgtcg ccttctccgc
     2581 acccgtcgcc gcaggcaatg aattcggcaa tttgtggatg cccaaatagc cgagcaatgc
     2641 aaagacgaat gcttccttcg catccgcatc gattccgaga tcgccggtcg ttgcaaattt
     2701 caaatcttca ggcagcgatg ccttcaggaa ccccatcaat gtctgattgt gcacaccacc
     2761 tccgctgatg atcacttcct tcacgtcatg cgactgtata tatctttgaa tctcagcagc
     2821 tatcgttttg gccgtcagca tcgtggcggt cgcgatttta tcggtggatg aaagcccatg
     2881 ctcatcgcct tccgcccaga atgtttcgac gaatgcagcc ccgaataatt ccctcccggt
     2941 gcttttaggc gctggagcat cgaaatattt atggtccatc agacgatcaa tccaatcctc
     3001 atcgatggtg ccgttcttcg ccagttcacc gtccttgtcg aaagagcttt gtccgccgga
     3061 atgccttgca acacaagcat cgatcaccat atttcctggc cccgtgtcat aggcgatgat
     3121 cggctgctcc gatccgagcc gcggcagcac tgtcatattg gcgatgccgc cgatattcag
     3181 aaggatacgt ccgaccgatt ccgattggaa taaaacctga tccgcaaacg gcactaatgg
     3241 agcgccttgc ccgcccacag ccatatcacg tgtccggaaa tccccgatga cggttttgcc
     3301 cgtgcgctcc gcgatgaccg cgatatcacc gatttgcatc gtattcggcc gagcatacga
     3361 acgatccggt tcaaccgccg gctcatgcca aatcgtctgg ccgtgggagc tgatgaggtc
     3421 gaccgtttcc aagccgatgc ccgcctcctg taaagctttc tgcaacgaat ctgcatatcg
     3481 ttcccccaga tacatattca tcgaggagac gagatgcaaa ggcgccgtcg gatccatcaa
     3541 cttatgcagt tcctgcttta gtccatcatc ataaggcatc gtaatgaaat gcagcaattc
     3601 atgacggatc tttccctcgt tgcgggtgaa atcgacaacc gcgatatcga ggccatccaa
     3661 ggatgttccc gacattaatc cacagacacg cattggcttc aatggcattc ccctttccaa
     3721 tatctctcat tgaaaacgct tactgtcatt ttagtccctt tgaaattaaa tatcaatact
     3781 aatttattat tctaaaaaga attttcaatc aatcaatcaa tagatactgg caatctgcca
     3841 ttgattgctg tttttcctgc taagtgttca gccacgactt caaacgccga ctgcgtgaat
     3901 tcatatgtgc aaatgatccg gttcgccttc gggaaataag ccgcgtcata cggcgaccgg
     3961 acggcaatga catcgaccgg cttcccgctt gccattaatc cttcgacgat gcgccgctgg
     4021 ttttcatgct tatcggcatt caacgtaagc atgacgatat ggttgtatct agccgcgagt
     4081 tccgtcatat ccatgtcgcc cggaccttca ccgtcaatct ccatcgatac aacgttcccg
     4141 tgaacttttt tcaactgctc cgccatcgtc aacgtcgcat accgtttatc ctcgaccatc
     4201 atcgcatatt cgtttttcgg atggatgagc agaacacgtt cctccgctgc aatcggtatt
     4261 cccggccctt tcacttccgt aatgccttgc acgtaaatct ccttcatttc atcatgatgc
     4321 ttcccggagc ctacaaattc ggcaaccgtt tgatttgctt cgatttcatc ccaagacgta
     4381 tacttcctcg cgagcttttc gatcctttca atcgactctt cgatccgccg ctcggaaatc
     4441 ctgccggtct cgacagcttc cgccaatcgg atgaccgcct gctcctgcag atcatgcaaa
     4501 tgggagacca tgacgaaatc cacacccgct tcgaccgcct gtacgcatcc ttccaccgta
     4561 ccgatccgct tcgcaatcgc gtccatctcc atgcaatccg tcgtgatgac gccgtcgaat
     4621 ccgagctctt cccggagaag tcccgttatg acagagtgcg ataacgtcgc cgggacattc
     4681 tcctcttttt cgagcgccgg aaaatagaca tgtgcgctca tgacagcatc cgcaccagcc
     4741 gccatgcatt ccttgaacgg cacgagctct accgcatgca gccggtcaag attatggtcg
     4801 atcaccggca agtccaaatg ggagtcgacg ctcgtatcgc catggcccgg gaaatgcttc
     4861 aatgtcgtca tgactcccgc ctcttgcatg ccgagcatcg aattcatcgc catttcggca
     4921 acagctgcag gattttcccc gaaggaacgg acgccgatga ctgggttctt cgggttgttg
     4981 ttaacgtcca cgacaggcgc caaattccag ttgatgccga gcgctttcaa ttccttgccg
     5041 gtcattaggc ccgctttcct ggcattcgcc ggatcttgcg tcgctccgag cagcattgca
     5101 ccaggaatga ttgtcgtccc ttcacccaat ctcctgacga cgccattttc ctgatcgatg
     5161 cagatgagca gcggcgtttc atagccagcc ttcttcgctt cagcttgcag gctgttcgtc
     5221 aacgcgagaa tttcttcggg cgtgccgata tttctcccga ataggatgat tcctccaata
     5281 tgatatgtgt gaatcaaatg tttaatttcc tccgacatcg taatgccttt gaatccggca
     5341 atcagcaatc gcccgacctt cttttgcagc ttcatgatgc ttccccctct taaaatctct
     5401 tcttaaatcg ggaatcccgg gatatgacct tcctttctaa cgtgcaagcc ccttgaattc
     5461 ttcaacgtct gcgcataagt cgtgcgggcc aaacagccgc gcatcgtccc aagtgcttta
     5521 taataatcat aatatcggaa actcgtggaa ttcatgatga accaatagca tgacaacgcc
     5581 ttcaaataag cgtcgaatga atccgtcaca tcgatgtaaa tatcaggctc atagccatcc
     5641 atgtcttccc agttctcact atggaagaca cggcgcaaac cgtgcggcgg aagtccttcc
     5701 aggtcgaatc cgggcagtga tgctttcaac catgcatcct gtacgatctt ttggcaaagc
     5761 gcatgatcgg gatgcatgct gttctcccaa tgtgtgatga cgaattccgg cttctccctc
     5821 cggaaaatcg tcgcgacctc tttgacgatt gcatcgttga atgtcaactc ggcatcccgg
     5881 tgcgcgagcg tgatgcttgt tccgcccaaa atggctgctg ccgcctcagc ttcacggatc
     5941 ttttgccgac ggtattcttc cacatgcata tgcggcggtg ctcccttttc ccctgccgtc
     6001 aaatgaagga atgtcacccg ataccccgcc tgtgcgtatt tatgggcgat tgcgcctgcc
     6061 tggatctccc cgtcgccgca atgcgccccg acaatcatga catgctgcat acctgatccc
     6121 cctgtgagaa cattgatgta tagatgatat tatgtaagcg gggcctcaag cggagcattg
     6181 caagatgccg tccgaagtac acacggttcg tcagcagcat gaccgtcaat tccttttccg
     6241 gatcgatata gaagctcgtt ccggtgaatc ccgtatggcc atacgtgctt tttgacatca
     6301 gatcccccgc cggactcgct ccgttccctt tcagttgcca tccaagacct ctgctttcat
     6361 ccgcaaacgg tgtgaaattg cgtctcgata acgcaagcca atgcggatcc aagattttct
     6421 ttccatcatg cacaccgtca ttttcaatca tttcc
//
LOCUS       DASULI010000373         2787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_109383, whole
            genome shotgun sequence.
ACCESSION   DASULI010000373 DASULI010000000
VERSION     DASULI010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109383"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..659
                     /locus_tag="VFX34_08015"
                     /pseudo
     CDS             36..659
                     /locus_tag="VFX34_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
     gene            1088..1933
                     /gene="panB"
                     /locus_tag="VFX34_08020"
     CDS             1088..1933
                     /gene="panB"
                     /locus_tag="VFX34_08020"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5564891.1"
                     /translation="MKSTTDFTKMKRLGEKIVMLTAYDHPSAQLAEEAGIDVILVGDS
                     LGMVVLGYDSTAAVTVEDMIHHGKAVRRGAKETFVVVDMPFATYHGSADRTLETAVRI
                     FQETGANALKLEGGGKVIDTIRLLTETGIPVVAHLGLLPQSAAVAGGYKVQGKTAEAA
                     EQLIQDAIACEAAGACMIVLECIPYQLAEQVSKAVAIPTIGIGAGAETDGQVLVFHDT
                     VKYGNHHIPKFVEAFADVGTDIRNGLKRYTSAVKSGEFPSEVHRFAMKEDELVALYGG
                     KNDVR"
     gene            1923..>2787
                     /gene="panC"
                     /locus_tag="VFX34_08025"
     CDS             1923..>2787
                     /gene="panC"
                     /locus_tag="VFX34_08025"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5564892.1"
                     /translation="MYVESTGILVIESIEELQDKLNRNECNSATVGFVPTMGYLHEGH
                     LSLVKHAKEQNDIVVMSIFVNPAQFGPGEDFESYPRDRVRDIRLASEAGVDIVFIPTV
                     QEMYPADGGIRILPGRQAEELCGASRPGHFDGVLKVVLKLFNIVDPDRSYFGMKDAQQ
                     LAIIETFVRDFNLRTAIVRVPIVREEDGLAKSSRNVNLSAVERLEAPAIYEALLAGAE
                     LFADGASPTAVEEQVVEQILSNSSGKIDYVKMLAYPGLGPVSDDSEEVILACAVKFSS
                     TRLIDNIIMSTK"
BASE COUNT          786 a          567 c          733 g          701 t
ORIGIN      
        1 atggaaccct tgtcatttcg taagaacaga cagccggcaa gggaagactg tcccgaccat
       61 ggaattcggc agctcaaaag ggcatttgga tgagcctgat cgtcaggcca tctttgatgc
      121 cgcagcaagc accgcaaatg acgcttgtcg cggctgtagc aatcgtcagg gcaattgaaa
      181 atatcgccgg tatagaagcg acaatcaaat ggccgaatga tattatgctt aatagccgaa
      241 aaataactgg catattgacc gagcttcaat cagaacctga tcgggtgaaa gcgatcattc
      301 ttggaatcgg aatgaatgtc aaccaagacg aagaagattt tcctttggaa cttaaagaga
      361 ttgcgacatc attgaagatt gcctgcggaa ggcatattga ccgcgcgaaa ctcattgctg
      421 agattcttgg atttattgaa ttatatacga agatgtatga aaaacatgga ttcggaccga
      481 ttaaattgct gtgggaagga tattccaata tttcgggcaa acgcatccga gcggtcatgt
      541 taaatgaaac ggtggaaggg actgctttag gcatctctga agaaggtctg ttggagcttc
      601 agcttgataa tggcgaggtc agaggcatct attccgcgga tattgttata aaagattgaa
      661 tgtatacaaa tctttcttta tttgctatag taattgcgaa gggcagtatc aactggaatt
      721 gcaccttcaa cgtcttttgc tcggcaaaaa aatagaatag tatctgcctt gatcttgtaa
      781 aagacaggga cggaggaaac caaaaatcga actcgcacaa tccttctgcc cttttttagg
      841 tcagaaggat tttttaactt aatccagcag aagtccctta cttataaaat ttcgggatga
      901 aagcgattac acaattattc agtgaaattc aaagccttgc tgaattaagt taaagcctcc
      961 ggtggatgtc acggattttt aggagagttt atcgagctat tttgataaaa atccggacgc
     1021 aattccgcca aggcgcaatt gatttatcgg ttttctccat tttgacaacc aaaagggaga
     1081 ggatacggtg aaaagcacta cggattttac gaagatgaaa aggcttgggg aaaaaatcgt
     1141 catgttgacc gcatatgacc atccatcggc acaattggcg gaagaggcag gcatcgatgt
     1201 cattcttgtc ggggattcgc ttggcatggt cgtattgggg tatgattcga ccgcagccgt
     1261 aacggtcgag gacatgattc atcatggaaa agcggtaaga cggggcgcaa aggagacatt
     1321 tgtcgtcgtc gacatgcctt tcgctacgta tcacggttca gctgatagga cgttggaaac
     1381 cgcggtccgc atcttccaag agactggtgc gaacgcgctg aaactcgaag gtggaggaaa
     1441 agtgatcgac acgatacgtc ttctgacgga gacaggaatt ccagtcgtcg cccatttagg
     1501 tctgcttccc cagtcggctg ctgttgccgg gggatataaa gtgcaaggaa agactgcgga
     1561 agctgccgaa caacttattc aggacgcaat cgcatgcgaa gcggcgggag cctgcatgat
     1621 cgtgctcgaa tgcattcctt atcagctggc ggaacaggtt tcgaaagccg ttgccatccc
     1681 gacgatcgga ataggggccg gggctgagac ggacggtcaa gtgctcgtct tccatgatac
     1741 tgttaaatac ggcaaccacc atattccaaa attcgttgaa gcgttcgcgg atgttgggac
     1801 tgatatccgt aatggtttga aaagatatac gtcagcagtc aagagcggcg aattcccttc
     1861 tgaagtacac cgtttcgcaa tgaaggaaga tgaactcgtc gcattatacg ggggaaagaa
     1921 tgatgtacgt tgaaagtact ggcatcctgg ttatcgaatc gattgaagaa ctccaagata
     1981 aattgaaccg taatgaatgc aatagcgcta cagtcggctt cgtcccgaca atgggctatt
     2041 tgcatgaagg gcatctatct cttgtcaaac atgcaaagga acagaatgat atcgttgtga
     2101 tgagtatatt tgtaaatccg gcacaattcg gacccggcga ggattttgaa tcctatccac
     2161 gggaccgggt aagggatatt cgtcttgcaa gcgaggccgg cgtcgatatt gtgttcatcc
     2221 cgacagttca agagatgtat ccggctgacg gcggcatccg catattgccg gggaggcagg
     2281 cggaagagct ttgcggcgct tcaagacccg ggcattttga tggtgttctg aaggttgtat
     2341 tgaaactctt caatatcgtc gatcccgacc gttcgtattt cgggatgaaa gatgcccagc
     2401 aactcgccat tatcgaaaca tttgtcaggg acttcaattt acggacagct atcgtacggg
     2461 ttccaatcgt cagagaagaa gatgggttgg ccaaaagctc acgaaatgtg aatttgtcgg
     2521 ccgtggaacg cttggaagcg ccagccatct atgaagcctt gctggccggt gcggaattgt
     2581 ttgccgacgg agcaagccca acggccgttg aggagcaagt ggtggaacag attctatcca
     2641 atagttctgg gaaaatcgat tatgtgaaaa tgcttgcata tcccggattg ggtcctgtaa
     2701 gtgacgattc agaggaagtt attttagcgt gtgctgtaaa gttttcttca acaaggttaa
     2761 tagataatat tattatgtca acaaagg
//
LOCUS       DASULI010000374         6437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_694949, whole
            genome shotgun sequence.
ACCESSION   DASULI010000374 DASULI010000000
VERSION     DASULI010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694949"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFX34_08030"
     CDS             <1..420
                     /locus_tag="VFX34_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX5564893.1"
                     /translation="QDNQLFGQSIGLNVPGTKRQLLVVIPGSVKAGVDLSKLTEKDVV
                     IDEKNKTAKLTLPAATFLGGAEIYFDQVEVYSYEGVFRQKADIEEAYELAAEAKNLII
                     EEATGQGVLKTAQFNAEKTLKEMFSFAGYDVTIEFKE"
     gene            426..1112
                     /locus_tag="VFX34_08035"
     CDS             426..1112
                     /locus_tag="VFX34_08035"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010287161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5564894.1"
                     /translation="MQKKRFGNDWDEVLAEQFQKPYYLQLRDFLKNEYVNHTIYPLME
                     DMWTAFKLTPFKDVKVVILGQDPYHGPGQAHGLSFSVKPGVRIPPSLRNMFKELQSDI
                     GCAIPETGTLTGWAKQGVLMLNTVLTVREGAAHSHRKQGWEQFTDEVIRRLSDREEPV
                     VFVLWGRPAQEKKKLIDLNKNAVIEAVHPSPLSASRGFFGSQPYSKANGILVEWGQTP
                     IDWCLSDDGL"
     gene            1129..1305
                     /locus_tag="VFX34_08040"
     CDS             1129..1305
                     /locus_tag="VFX34_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5564895.1"
                     /translation="MAVNCFHCQYFFVTWDPKSPRGCKAYGFKTRELPSIVVRRSSGM
                     ECMKFEPKKGDARR"
     gene            1302..1562
                     /locus_tag="VFX34_08045"
     CDS             1302..1562
                     /locus_tag="VFX34_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwdI family protein"
                     /protein_id="HEX5564896.1"
                     /translation="MISTDRIIEEMQRQLNYAKTADDDHAVREALTAIRALCQVVLVS
                     DKKEEPIITPRTMQITHKPRISSLEGRPLEEEDANGGSIFDF"
     gene            1623..2003
                     /locus_tag="VFX34_08050"
     CDS             1623..2003
                     /locus_tag="VFX34_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX5564897.1"
                     /translation="MPFFIIAGAVNAALAVALGAFGAHALKERLSERYLAIWETAVQY
                     QMFHAIGLIAVGILMSTSLFGPSTALSWAGYLLLAGIIIFSGSLYILSLSGIGILGAI
                     TPIGGVAFIVGWIMLIVATVKFGK"
     gene            2317..2628
                     /locus_tag="VFX34_08055"
     CDS             2317..2628
                     /locus_tag="VFX34_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564898.1"
                     /translation="MVVSRLGSKQYLYAALLLLVLFSNYFVYRLPAIPMPADSRAVVL
                     GSLLDLAIVAPLLILAMTRKKGFTLKRFFTFMVLGLVAARFVVPGEYFEPFKFVPYVA
                     I"
     gene            2638..3360
                     /locus_tag="VFX34_08060"
     CDS             2638..3360
                     /locus_tag="VFX34_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564899.1"
                     /translation="MLLLVELGLLFLLAMHLPKIIKEVRGANAEALFAFPALVKEKVS
                     AHPLVALITAESLMFYYAFASWKRKPPVGEDKFTLHQKTSMLIFYIMLVHAIVIETVG
                     IHWWLHGKSMILSIVLLVLNVYSVIYFIAEIQTVRLNPLAMKDDRIQISLGLGRRMEI
                     PYDAIERIEWFEDTEQCNLKEAGLIEFIAKDMEGKPNCILHFRRPLQATLFMGKEKEF
                     RTAAIRLDEPKNFRHALEKKLG"
     gene            complement(3521..3868)
                     /locus_tag="VFX34_08065"
     CDS             complement(3521..3868)
                     /locus_tag="VFX34_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein GerQ"
                     /protein_id="HEX5564900.1"
                     /translation="MVQYYYYPGFQQQQMQMPATPPQIPAGGRPPFREESYIENILRL
                     NRGKPGIFHFSFEHAIEPGKNTLAIPGMVEAAGRDHVILSTASGKRYLMPMIYFDYAE
                     FNEELNYFNQQPM"
     gene            4040..4891
                     /locus_tag="VFX34_08070"
     CDS             4040..4891
                     /locus_tag="VFX34_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016129695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5564901.1"
                     /translation="MQIENNIPVFLEFWESSEQPRLADLESYLNEHSKIYEKYFPMHC
                     PRTDERLTAALQKYEGKMDDIRAISDSLPAIIEEVDEKFERTFGLGLELSFKLMVGTF
                     GSNAFVTRDNRREIYFAVEKLSAERDHLKVITAHEIGHVTHFALATRDGMDWSAVDWG
                     HGLTNLFMEGAATYLSKGIVPGLQKPVYFSFDDEGDPWVKCYEENKSELKRRFLEDAS
                     GDWDMEKEREWFRLSGGAYFGYNRLGYVLGTDYVEQLVGRIGEDAALTFWNGNDLKAD
                     ILEWLRK"
     gene            4981..5763
                     /locus_tag="VFX34_08075"
     CDS             4981..5763
                     /locus_tag="VFX34_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5564902.1"
                     /translation="MPTFNANGTEIYYQEEGEGFPLVLLHGLTSNSEMFRHEIDKFKK
                     NYRVIAPDARGHGNSEKPPHYELKDHIGDVISLVDHLGLDSFHLIGVSMGSYIAQGVA
                     IEMGDRVKKLVLVATKSHGEQASMEELYERHADEMEGKTPIDKMVAATKYMFHNRKAV
                     RKWLHETAENSEQLTLHEQAIASKALEGFDFREDLHRIKAEALIISGLHDGLNPSDYG
                     EETAQLIPASVFLEFKLSGHAPNVEQPVLFMEVVSNFLESKD"
     gene            complement(6019..>6437)
                     /locus_tag="VFX34_08080"
     CDS             complement(6019..>6437)
                     /locus_tag="VFX34_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX5564903.1"
                     /translation="NYLAGESDEDPYQNPYVRGRLYPELPRNQYICFYPMDKKRDGED
                     NWYMLSMDKRKELMRSHGMIGRGYAGKVKQIISGSVGFDDFEWGVTLFSDDVLQFKKL
                     VYEMRFDEVSARYGVFGSFFIGTLLDEEKKKSFFTI"
BASE COUNT         1633 a         1538 c         1714 g         1552 t
ORIGIN      
        1 caggacaacc agttgttcgg acaaagcatc gggttgaatg tgccggggac gaagcggcag
       61 ctgctcgtcg tcattcccgg atcggtgaag gcgggcgtcg atttgtccaa gctcaccgaa
      121 aaagatgtag tcatagatga aaagaataag accgcgaaac tcacattgcc ggccgcgacc
      181 ttcctcggtg gtgcggaaat ctatttcgac caagtcgaag tctattcgta cgaaggcgtc
      241 ttccgtcaga aagcggacat cgaagaggcg tacgaactcg ctgccgaggc gaaaaatctc
      301 atcatcgagg aagctaccgg ccaaggcgtg ctgaagacgg cgcaattcaa tgcggaaaag
      361 acgttgaagg aaatgttttc attcgcgggt tacgacgtga cgattgaatt caaggagtga
      421 gatgcttgca gaaaaagaga ttcggcaacg attgggatga agtgcttgcg gagcaattcc
      481 agaaaccgta ttacttgcag cttcgtgatt ttctcaaaaa tgaatatgta aaccatacta
      541 tttatcccct catggaggat atgtggacgg ctttcaagct gaccccgttt aaggatgtga
      601 aagtggtcat tctcggacag gatccgtatc acgggcccgg acaagcccat ggcctcagct
      661 tttcggtgaa gcccggcgtc aggattccgc caagtttgcg gaatatgttc aaggaactgc
      721 aatcagatat cggatgcgcc attccggaaa cggggacgct gacgggctgg gcgaaacagg
      781 gcgtgctcat gctgaatacc gtcctcaccg tgagggaagg ggcggcgcat tcccatcgga
      841 agcaaggttg ggagcagttc accgacgaag tgatccgccg actgtccgat cgggaggagc
      901 cagtcgtttt cgtcctttgg gggcgtccgg cgcaggagaa gaagaagctg attgatctga
      961 ataaaaatgc cgtcatcgaa gcggtccatc cgagcccgct cagcgcaagc cgcggctttt
     1021 tcggcagcca gccctactcc aaggcgaacg gtattttggt agagtggggt caaacgccga
     1081 tcgactggtg cctttccgac gacgggctgt aagggaagag gtggccgcat ggctgtaaat
     1141 tgttttcatt gtcaatactt tttcgtaaca tgggatccaa aaagcccacg tggctgcaag
     1201 gcatatggtt tcaagacacg ggaactgccg tcgatcgtcg tcaggcggtc atccggcatg
     1261 gaatgcatga aatttgaacc gaagaaaggg gatgcaagaa gatgatttca actgacagga
     1321 tcattgagga aatgcaacgc cagctgaatt acgcgaaaac agcagacgac gaccatgccg
     1381 tccgcgaggc actcacggcg atacgggcgc tctgccaagt cgtactggtc agcgataaga
     1441 aagaagagcc gatcattacg ccgcgaacaa tgcagatcac ccataagccg cggatttcgt
     1501 cattggaagg caggccgctt gaggaagagg acgcgaacgg cggttcaatt tttgatttct
     1561 gacggcgata gtcgttatca taagaaggga agaaatttca caagcagaaa gtaggaaatc
     1621 atatgccatt tttcatcatc gcgggcgctg tgaacgcggc gcttgctgtt gcactcggcg
     1681 cattcggcgc gcatgcactg aaggaacgat tatccgaacg gtacctcgct atatgggaga
     1741 cagccgtcca atatcaaatg ttccatgcaa tcggacttat cgcggtcggc atcctcatga
     1801 gcacatcgct tttcggcccg tcgaccgctt tgtcatgggc aggctatctg ctgctcgccg
     1861 gcatcatcat cttttccggc agcctttata tcctaagcct atccggcatc ggcattctcg
     1921 gcgccatcac cccgattggc ggcgtcgcct tcatcgtcgg ctggatcatg ctcatcgttg
     1981 caacggtaaa gttcggaaaa taacaggaaa acggtttgaa gagcaccagt tcttcaaacc
     2041 gtttttttat ttgaatcgcg tcaaggggaa gacgcgcgtc caagatcgtt tcccgcgctc
     2101 aaagtcttcc gttctgcgct caacgtaagt cctcccgcgc tcaagaaatc ggatccgcgc
     2161 tcaagaaagc acctcccgcg ctcaagaatc ctccatccgc gctcaagact ccacctccgc
     2221 gcgtccaaac catcaaaaat ctccataaat ctgtaaaaac tatcctgaaa tgcaatccgc
     2281 cttcattagt aaactaaaag aaaaggggga gattccatgg tggtgtcgcg tttaggttcg
     2341 aagcaatatt tatatgctgc tttgctattg ctcgttctat tcagtaatta ttttgtctac
     2401 cggctgcctg cgattccgat gccagctgat tcccgagctg ttgtgctcgg atctttgctg
     2461 gacctcgcaa tcgttgcgcc gcttctcatc ctcgcaatga cgcgcaagaa aggctttacg
     2521 ctgaaacgct ttttcacatt catggtgctc ggcctcgttg ctgcgcggtt tgtcgtaccc
     2581 ggtgaatatt ttgaaccgtt caagttcgtt ccctacgtcg cgatctgatt cgaaggattg
     2641 ctcctacttg ttgaactggg tctcctgttt ttattggcaa tgcatttgcc gaaaatcatc
     2701 aaggaagtga ggggagcaaa cgccgaagcc ctttttgcat tcccggcgct tgtgaaggaa
     2761 aaggtttcgg cccacccgct cgttgcactc atcacagctg aatcgcttat gttctactat
     2821 gcattcgctt cctggaagcg gaagccgccc gtcggggaag acaaatttac attgcatcaa
     2881 aagacaagta tgctcatctt ttacattatg ctcgtccacg caatcgtcat tgaaacggtc
     2941 ggcatccatt ggtggctcca tggaaaatca atgatcttgt ccatcgtcct gctcgtcttg
     3001 aatgtctatt ccgtcattta ttttattgcg gaaattcaaa ccgtccgtct gaacccgtta
     3061 gcaatgaaag acgatcgaat acagatttcc cttgggctcg ggcgccgcat ggaaattccc
     3121 tatgacgcga ttgaacgaat cgaatggttt gaagacacgg agcaatgcaa cttgaaggag
     3181 gcgggtctta tcgaattcat tgcgaaggac atggagggta agccgaattg catccttcat
     3241 ttcagacgac cgctgcaggc gacccttttc atggggaagg aaaaggaatt ccgtacggca
     3301 gcaatccgtt tggacgaacc gaagaacttc cggcatgcgc ttgaaaagaa gttgggataa
     3361 taaaaaatct tccactgcga gggcaatgga agattttttt catctagact ccgggtggca
     3421 actgcttggg tcataagtca gctccgctgt acggcaaaaa tcgccgtttc gcggatctgt
     3481 cttatgcctg tcgcaattaa ccgccaccct ccgcttttgt tcacatcggc tgctggttga
     3541 aataattcag ttcttcgtta aactccgcat agtcgaaata gatcatcggc attaaataac
     3601 gtttgccgga agctgtgctt aagatgacgt gatcccgacc ggccgcttcc accatccctg
     3661 ggattgccag cgtattcttt ccgggttcga tggcatgttc gaaggagaag tggaatatgc
     3721 cgggtttgcc gcggttgagg cggaggatgt tttcgatata cgactcctcc cggaacggtg
     3781 gccttcctcc agcaggaatc tgtggcggcg ttgcaggcat ttgcatttgc tgttgttgaa
     3841 aacctggata ataatagtat tgaaccatgt tttccatcct ttctgttctc aattacttac
     3901 acgtgcgggc aatcctcttc agtagggaaa atgaagcaat gcaatttgca tcttcataat
     3961 cccctcggtt caactatatg catatgtgct ttcattgatg actgaaaaat atgtatactg
     4021 gtggggaggg ggagagcata tgcagatcga aaataacatt ccggttttct tggagttctg
     4081 ggaatcaagc gaacaaccga ggcttgccga cttggaatcg tatctcaatg agcattcgaa
     4141 aatctatgaa aagtattttc cgatgcattg tccgaggacg gacgagcgtt tgacagcggc
     4201 gctgcaaaaa tacgaaggca agatggatga cattcgtgcc atttccgaca gtttgccagc
     4261 cattattgag gaagtggacg agaagtttga gcggacgttc ggattagggc tggagctttc
     4321 attcaaactg atggtcggca cgttcggatc gaatgcattc gtaacgaggg acaatcggcg
     4381 tgaaatctat tttgctgttg aaaagctttc cgcggagcgc gaccatttga aagtgatcac
     4441 tgcgcatgaa atcggccacg tcacacattt tgcgcttgcc acgcgtgatg ggatggattg
     4501 gtccgccgtc gattggggac acggcttgac gaatcttttc atggaagggg ctgcgacgta
     4561 tttatcgaag gggattgtcc cggggcttca gaagccggtc tatttttcat tcgacgacga
     4621 aggggaccca tgggtgaagt gctatgagga gaataaatcc gagctgaagc ggcgattttt
     4681 ggaggacgct tcaggggatt gggatatgga gaaggaaagg gaatggttcc gtctatcggg
     4741 cggcgcctat ttcggttata atcgcctcgg ttatgtcctc ggcacagact acgtcgaaca
     4801 gcttgtcggg cggatcggcg aggatgctgc actgacattt tggaatggga atgatttgaa
     4861 ggcggatatt ttggagtggt tacggaagtg aaagggaaag ggccgtatgg atggctcttt
     4921 ttttatttat tgaaaatagg gtatgaataa ggcaaagtgc ttttacggag gagtggtacg
     4981 atgccgacat tcaacgcgaa tggaacagag atttattacc aggaggaagg ggaagggttt
     5041 ccgctcgtgc tgctgcacgg tttgacgagc aatagtgaaa tgttccgcca tgaaatcgac
     5101 aagttcaaga aaaattatcg ggtcatcgca ccggacgccc gggggcatgg gaattcggag
     5161 aagccgccgc actatgaatt gaaggatcat atcggtgatg tcatctcgct cgtcgatcat
     5221 ctcggactgg attcatttca ccttatcggt gtgtcgatgg ggagctatat agcgcagggg
     5281 gtcgccatcg aaatgggcga ccgcgtgaag aagcttgtgc ttgtcgcgac gaaatcccat
     5341 ggcgagcagg cttcgatgga agagctttac gaacggcatg ccgatgagat ggaagggaag
     5401 acgccgatcg ataaaatggt cgccgcaacg aaatacatgt tccataaccg gaaggcggtc
     5461 aggaagtggc tgcatgaaac ggcggagaac agtgagcaat tgacgttaca tgagcaggcg
     5521 attgcatcga aggcgttgga aggattcgat ttccgggagg atctgcatcg gatcaaagcc
     5581 gaggcgctca tcatcagcgg cctgcacgac ggactgaatc cgtccgatta cggtgaagag
     5641 actgcccagc tcataccggc gtccgttttc ctcgaattca aattatcggg ccatgcgcca
     5701 aatgtcgaac agccggtcct gttcatggaa gtcgtctcga acttcttgga atcaaaagat
     5761 taaatggtca gaggcattca acgcgacaaa ctggacttca ttcacgcata aacaccgcga
     5821 aaagcgcata aatccatcta gacacgcata aaaagttgca gacgcgcata aatccggcta
     5881 ttcactcata aattgttatt tccacgcata aaccgctaca aacacgcata aatccgtaca
     5941 gaagcccaaa aagcgcataa tcatgcatat atccatcaat caaaaaagag cccctagagg
     6001 ctctttttca tcaaacgatt aaattgtgaa aaacgacttc ttcttctcct catcgagcag
     6061 cgtaccgatg aagaacgaac cgaacacgcc gtagcgcgcg ctcacttcgt caaagcgcat
     6121 ttcgtaaacc aatttcttga actggagaac atcatccgag aacaatgtca cgccccattc
     6181 gaaatcgtcg aaaccgacgg agccggaaat gatctgtttc actttgccgg cgtatccgcg
     6241 gccgatcatt ccgtggctgc gcatcaattc cttgcgcttg tccatgctga gcatatacca
     6301 gttgtcttcg ccatcgcgct tcttgtccat cggatagaag cagatgtatt ggttgcgcgg
     6361 cagctccgga tacagacgtc cacgcacata cgggttctga tacggatctt catccgactc
     6421 gcccgcaagg tagttgg
//
LOCUS       DASULI010000375         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_809182, whole
            genome shotgun sequence.
ACCESSION   DASULI010000375 DASULI010000000
VERSION     DASULI010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809182"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1291
                     /locus_tag="VFX34_08085"
     CDS             161..1291
                     /locus_tag="VFX34_08085"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX5564904.1"
                     /translation="MNELGFGTLKKVLVVDDSAFMRKLISEMLSKHPLLEVIGIARNG
                     KDALAKVAELRPDVITMDIEMPLMNGLEALKQIMEQQPTPVVMLSSTTETGAENTMIA
                     MEYGAVDFVAKPGGAISLNLHEVEREIVGKVIAASKVEISNLIKQPTPRVEPESTLKE
                     ATSKPSVSFVNKIDSLQEKKISKAHETFVIIGTSTGGPRALQEVLTKLPSNINAPILV
                     VQHMPPGFTKSLAQRLDGLSEIRVKEAEDGEFLLKGVAYIAPGGKHLKFEKCLGGFCV
                     RLDDTVPPRMGHRPSVDVLLESAADKRDLQYVTTIMTGMGHDGREGMQQLKAACKTIT
                     IAESEKTSVVYGMPKAIKEAGLADRVVDVQCIAAAITEILHS"
     gene            1304..3343
                     /locus_tag="VFX34_08090"
     CDS             1304..3343
                     /locus_tag="VFX34_08090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX5564905.1"
                     /translation="MDTNQYLEMFLEESKEHLQACNEHLLELEKNPEDLAIVNEVFRS
                     AHTLKGMSATMGYEDIADLTHKMENVLDAIRNSKIRVSTEILDIVFKAVDYLEEMVMD
                     IEVGGTGKKNVQELVSALNRIETGQPAIDSPEMETAASTMAIEPDKQRPVLTYDDYEM
                     TVIGQSQEQGFHAFEISVTLRDDCLLKAARVFMVFEILEKSGEIVKTKPSVEQLENED
                     FDEVFTIVLITKEEASSLEAKVMKVSEVDRVDIHPVASASQPKGQQPVVSEADSVEKT
                     STESKKDEPAQKGRRAVNAHNANKTIRVNIDRLDILMNLFEELVIDRGRLQSIASELH
                     NPDLNETVERMTRVSGDLQNIILNMRMIPVETVFNRFPKMVRQLARDLDKKINLEIIG
                     AETELDRTVIDEIGDPLVHLIRNALDHGIESPAERLAKGKPEEGTVTLRAYHSGNHVF
                     IELEDDGAGVNKERVLRKALERGVVTEEMAATLTDRQIAELILASGFSTAEKITDVSG
                     RGVGLDVVKSTIESLGGYITIDSTEGQGSLFQVQLPLTLSIISVMLVNLDKDIYAIPL
                     SSIIETAIIQTSDILNAHNQQVIDFRGSIVPLVNLKGIFEMEDAQEKDEFQSVVIVRK
                     GDKMAGLVVDSFIGQQEIVLKSLGNYLQSVFAISGATILGNGQVALIVDCNALIK"
     gene            3360..>3694
                     /locus_tag="VFX34_08095"
     CDS             3360..>3694
                     /locus_tag="VFX34_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008180734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX5564906.1"
                     /translation="MAEVMESSKEMKVIVFQLMDKEYAVGVDVVESIEKLISITRVPK
                     TPSYIKGVINLRGVVTPIVDLRERFGMEVKPLDDSSRIIIVSLEEFEVGLIVDSANDV
                     IDIPVVSIEP"
BASE COUNT         1089 a          738 c          965 g          902 t
ORIGIN      
        1 gcggtcgtcg cccaaaaacc gtttatattg ctctttccga acgctcccat ctcgaagcgt
       61 atggcagcaa tcgcaagtga atttatcaaa gcggatcaga cggaggaaaa acaaggaacc
      121 ggcttccttc aaaagttgaa agggattttc ttgaaagggc gtgaatgaat tgggattcgg
      181 cactctgaaa aaagtgcttg tcgtcgatga ttccgcgttt atgagaaaac tgatatcgga
      241 gatgctttcg aaacatccgc tgctggaagt gatcgggatt gcacgcaatg gaaaagatgc
      301 tcttgcaaaa gtcgcagaac tccggccgga cgtcatcacg atggatatcg aaatgccgct
      361 catgaatgga cttgaggcat tgaaacaaat catggagcaa caaccgacac cagtcgttat
      421 gctctccagc acgaccgaga cgggtgcgga gaacacgatg attgcgatgg aatatggtgc
      481 ggtcgatttt gtcgcaaagc cgggcggagc catttcactt aatttgcatg aagtggaacg
      541 tgaaattgtc gggaaagtca tagctgcttc gaaagttgaa atttcaaatc ttattaaaca
      601 gccaacaccg cgagtggagc ctgaatctac cttgaaggaa gcgacatcca agccatccgt
      661 ttcatttgtg aacaaaatag attcattgca agagaaaaag atttccaaag cgcatgagac
      721 ttttgttata ataggtacat cgacaggggg acctcgggca ctgcaagaag tgctcacaaa
      781 actgccatca aatattaatg ccccgatttt agtcgtccag catatgccgc ctggatttac
      841 aaaatcattg gctcaaagat tggacggctt aagtgaaatt cgggtgaagg aagccgaaga
      901 tggagaattt cttctgaaag gcgttgccta tatagcccct ggcggcaagc atttgaaatt
      961 cgaaaagtgc ctgggaggct tttgtgtccg gcttgatgat accgttcctc cacggatggg
     1021 gcatcgtccg tcagtagacg ttctattgga gtcggctgcg gacaagcggg acttgcaata
     1081 tgtaacaacc atcatgacag ggatggggca tgatggcagg gaaggtatgc aacaactgaa
     1141 agcagcttgt aaaacgatta cgatagcgga atctgaaaag acttctgtcg tctatggcat
     1201 gccgaaagcg ataaaagaag ccggcctcgc cgatcgtgtc gttgatgtac aatgcattgc
     1261 ggcggcaatt acggaaatct tacattcttg agggggcgtc atgatggata cgaatcaata
     1321 ccttgaaatg tttcttgaag aaagcaagga acatcttcaa gcgtgcaatg agcacttatt
     1381 ggaactggaa aagaatccgg aagatctggc tatcgtcaat gaagttttcc gttcagcaca
     1441 tacgttgaaa gggatgtccg caacgatggg atacgaagac atcgccgatt tgacgcataa
     1501 gatggaaaat gtgctcgatg cgatccggaa ttcgaaaata agagtttcta cagaaatact
     1561 agatattgtt tttaaagctg tcgattattt agaagaaatg gtaatggata tcgaagtggg
     1621 cggcacaggg aaaaagaatg tacaggagct tgtgtctgca ttgaatagaa tagagaccgg
     1681 ccaaccggcg attgattcac cggaaatgga aacggctgct tctacaatgg caatcgagcc
     1741 ggacaaacaa agaccggtgc tcacttacga tgattatgaa atgacggtca ttggacaatc
     1801 acaggaacaa ggcttccatg cattcgaaat ttccgttaca cttcgcgacg attgtctgct
     1861 taaagcggca agggttttca tggttttcga gattcttgag aaatcaggtg aaatcgtcaa
     1921 aaccaaacct tccgtggaac agttggaaaa tgaggatttc gatgaagtgt tcacgatcgt
     1981 actgatcact aaagaagaag caagttcgct tgaggcaaaa gtgatgaaag tttctgaagt
     2041 ggatcgggtc gatatccatc ctgtggcaag cgcatcacag ccgaaaggac agcagcctgt
     2101 tgtgagtgag gctgattccg ttgagaaaac ttcgacagaa tccaagaagg atgaacccgc
     2161 tcaaaaagga cgccgtgcag tgaatgcaca taatgcaaac aaaaccattc gtgtcaacat
     2221 tgaccgattg gacatactca tgaatctttt cgaagaattg gtcattgaca ggggacgttt
     2281 gcagtcgata gcaagtgaat tacataatcc tgatttgaat gaaaccgtcg agcgcatgac
     2341 ccgggtttca ggcgatcttc aaaacatcat cttgaacatg cgcatgattc cagtggagac
     2401 cgtattcaac cgcttcccta aaatggtgcg tcagcttgca cgtgatctcg ataagaagat
     2461 caatcttgaa atcatcgggg ctgaaactga attggacagg acggttatcg atgaaatcgg
     2521 ggatccgctc gtacatttga tccggaatgc attggatcat ggcattgaga gcccggcgga
     2581 aagacttgcg aaagggaagc ctgaagaagg tactgtcacc ttgagggctt accattcagg
     2641 caatcacgta ttcattgagc tggaagacga cggggcaggt gtcaataagg agcgggtttt
     2701 acggaaggcg cttgaaagag gcgttgtcac tgaagagatg gcggccacgc tgacagatcg
     2761 tcagatagcg gagctcattt tggcatctgg attttccact gccgaaaaga ttactgatgt
     2821 atcagggcgc ggggttggcc tggatgtcgt aaaaagcaca atcgagtcac ttggcggata
     2881 tattacaatc gactcaacgg aaggacaggg atccttattc caagttcaat tgccgctgac
     2941 attatccata atttcggtca tgctcgtcaa tttggataag gatatttatg caatcccgct
     3001 ttcatcgatc atcgaaactg caatcattca aacttccgat atcttgaatg cgcataacca
     3061 acaagtgatt gatttcaggg gaagtattgt tccactcgtc aacttaaaag gaatcttcga
     3121 gatggaggat gcacaggaga aggatgaatt ccaatctgtc gtcatcgtcc gtaaaggtga
     3181 taagatggcc ggcctcgttg tggattcctt tatcggacaa caagagatcg ttttgaaatc
     3241 acttggaaat tatctgcaaa gtgtatttgc catttcggga gctaccattt tagggaacgg
     3301 acaagttgcg cttatcgtgg attgcaatgc attgatcaaa taggcgggag gggaaaagca
     3361 tggctgaagt aatggaaagc agtaaagaaa tgaaagtgat cgtgttccag ttgatggata
     3421 aggagtatgc cgtaggcgtc gacgttgtcg agtcgatcga aaaactcatt tcgattacga
     3481 gagttccgaa aacgccttcc tatataaaag gcgtcattaa cttaaggggc gttgtcacgc
     3541 cgattgtcga tttgcgtgag cgattcggta tggaagtgaa acctctcgat gacagcagca
     3601 gaatcatcat cgtttcgttg gaggaattcg aagtggggct gatcgtcgat tcagcgaatg
     3661 atgtgatcga tattccggtc gtttccatcg agcc
//
LOCUS       DASULI010000376         5565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1089783,
            whole genome shotgun sequence.
ACCESSION   DASULI010000376 DASULI010000000
VERSION     DASULI010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089783"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1138)
                     /locus_tag="VFX34_08100"
     CDS             complement(2..1138)
                     /locus_tag="VFX34_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007431450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HEX5564907.1"
                     /translation="MNPLLSNKNLQHERDYSRYPFIVIWEVTRACALKCLHCRAEAQY
                     KPNPWQLSFEEGKKLIDEIALMDNPLFVFTGGDPLMRPDLFELAKYAIEEKNLPVSMT
                     PSATPKVTREAIRKAKEVGLSRWAFSLDGSCAEIHDHFRGTRGSFDRTMKGIEYLKEL
                     NIPIQVNTTISNYNLHDLEAIAEKVKDMGAVLWSTFFLVPTGRGQEKDMISAEEHEKV
                     MKWLYQIQQKMPYGVKTTEAPHYRRVFLQEEQDAAGKMDDREAKRKDVLGRAPKPVND
                     GDGFVFISHIGEVYPSGFLPVSCGNIREQSLTDIYQNSPIMRELRDKSLLKGKCGVCE
                     FNKICGGSRSRAYAMTGDYLESDPSCSYIPKALRKKELEVTTHE"
     gene            1355..1561
                     /locus_tag="VFX34_08105"
     CDS             1355..1561
                     /locus_tag="VFX34_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5564908.1"
                     /translation="MFMSDLANIPWSLVLPIVVLQLILMVVALVDLIRHQRTNGPFIM
                     WIFIILLGNIIGSILYFIFGRKQA"
     gene            1558..2460
                     /locus_tag="VFX34_08110"
     CDS             1558..2460
                     /locus_tag="VFX34_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564909.1"
                     /translation="MTQLLQVNDLSKIYGSHKAADSITFELAEGTSTALIGPNGAGKT
                     TTLSMLAGLVTPTSGSILFRGKPKPDIRASIGYLPQYPKFFPWLTALEFTEMAAVLSG
                     VETRQALAEAKKTLEFVGLGDAMKKRTGTLSGGMKQRLGLAQAIVHGPELLLLDEPVS
                     ALDPVGRRQVMNLLKELQQKTTILYSTHILNDAEEMTDQLLFLRKGVLVENGSLETIR
                     EKYANPRILIEFDSVEEADRFTALSPWAVKMEKKQVSIDAVKEGVGTNEVIEQLHTES
                     LQIRKVELQTASLEEIFMKVVMEG"
     gene            2457..3239
                     /locus_tag="VFX34_08115"
     CDS             2457..3239
                     /locus_tag="VFX34_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5564910.1"
                     /translation="MSSLNVLLRKEWRENIRNFKLYWIPIVFIIFGVIEPITNHFLPE
                     IMKSVGNLPEGTEFAWPEFRGEDIFVSLMSQYQMIGILIIILAFMGSISGERKSGTAT
                     LLYVRPMSFGHYFFSKWIVVNAIVLASVWLGFFAAWYYIGILFNSVAAGEVFAFVGIY
                     SLWLVFAVTVVLALSAWLPTGAVAGLSILVLIVFQIIDGVVGMYWTVTPWKLSSYAPN
                     AFSTFADQSAFWMSVGLTVALIIALVFFGIYMTKRNAAKTLV"
     gene            complement(3329..3565)
                     /locus_tag="VFX34_08120"
     CDS             complement(3329..3565)
                     /locus_tag="VFX34_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564911.1"
                     /translation="MLSLNKKKPFTSEDRKDAEGPFMDNPENLPTDKESIYDMHEDMQ
                     NVDPIPLEDVRLEEHEEKHHRETKSSSSSEEKYK"
     gene            3697..4197
                     /locus_tag="VFX34_08125"
     CDS             3697..4197
                     /locus_tag="VFX34_08125"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX5564912.1"
                     /translation="MEKRYVGTQNKIDAALYKITELTSQGYSEDQISAVANTDDDFAQ
                     LRRQTFVSLEGQGKEGFLDRFKGTFLDEQPKQARYFEEMGFSREEADQIYNDMKDGGI
                     AIFVDNVEPDIAIEGRGGGEVEDTERDSRAQLTDSGEMLAGDDDILQPRDTNAGTVPR
                     INTDHL"
     gene            complement(4252..4455)
                     /locus_tag="VFX34_08130"
     CDS             complement(4252..4455)
                     /locus_tag="VFX34_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5564913.1"
                     /translation="MMKERGYSDKVKGAVNKVKGEAKDQYGNATDDAMLQAEGKMDKL
                     KGEAQQEIGEFKDRFGNHKNQRR"
     gene            4662..5063
                     /locus_tag="VFX34_08135"
     CDS             4662..5063
                     /locus_tag="VFX34_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564914.1"
                     /translation="MIRKGRFAVWNGKEYPLLSQRRKYYLQSVNPEDKEIGFVEMGTR
                     GYFFKEISTDDLEDAYEVFPYALLSGYRFALESYEKETRIVTFVTNNPFAQKKVPVSL
                     HGNEFRIDIPMDQVEILEDRIPILGFESINR"
     gene            complement(5073..>5565)
                     /locus_tag="VFX34_08140"
     CDS             complement(5073..>5565)
                     /locus_tag="VFX34_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5564915.1"
                     /translation="LQEDGSFEIVSSGHRNPQGLAWNSKGKLYSSEHGQSANDEINEI
                     VQGGNYGWPEIEGNESKEGMLSPLLTSGASATWAPSGMTFHKGMLYVAALRGKAILVI
                     DPETMEITRRIEGYGRIRDVYSDGESLYFISNNTDGRGTPSESDDILYELVNVKKIAD
                     EGM"
BASE COUNT         1443 a         1210 c         1318 g         1594 t
ORIGIN      
        1 attattcatg ggttgtcacc tccagctcct tttttcgaag ggcttttgga atgtaggaac
       61 aggaagggtc gctttccaaa tagtcaccgg tcatggcgta tgcccgtgac ctggagccac
      121 cgcagatttt gttgaactca cagacaccac acttgccctt taatagggat ttgtcgcgta
      181 attcccgcat gatgggggag ttttggtaaa tgtccgtgag agactgttcg cggatatttc
      241 cacaagagac cggtaaaaag ccgctcgggt acacctcgcc gatatgactg atgaagacga
      301 aaccatcacc gtcgttcacg ggctttggtg ctcttcctaa cacatctttc cttttcgctt
      361 cccgatcatc catttttccc gcagcatcct gttcttcctg aaggaatacc cgtcggtaat
      421 gaggggcttc ggtcgttttc acgccataag gcattttttg ttggatttga tagagccatt
      481 tcatcacttt ttcatgctct tctgcactga tcatatcctt ttcctgtcca cggcccgtcg
      541 gcacaaggaa aaatgtgctc catagaacgg ctcccatgtc tttcaccttt tcggcaattg
      601 cttcgaggtc atgcagattg tagttggata tggttgtatt cacttggatc gggatgttta
      661 gttcttttaa atactcgatt cctttcatcg tccggtcaaa ggatcctctt gtcccgcgga
      721 aatggtcatg gatttctgcg caggagccat ccaaactgaa agcccaacgc gataatccga
      781 cttctttcgc tttccggatc gcttcacgtg tcactttagg ggtagcgctg ggcgtcatcg
      841 acacgggaag atttttctcc tcgattgcat acttggcgag ttcaaacaag tcaggtctca
      901 ttaacggatc cccgcccgta aagacaaata acggattatc cattaatgcg atttcgtcaa
      961 tcagtttttt cccctcttca aaagacaatt gccatggatt tggcttgtat tgggcttcag
     1021 cccggcaatg caagcatttt aaggcgcaag cacgggtcac ttcccaaata acgatgaatg
     1081 gataccggga atagtccctt tcatgctgca aattcttatt actgagtaat ggattcataa
     1141 agaacactcc taatttatga ttgtgactta ttgattccta agttgggtac aggtttactt
     1201 tcaccttcat cataaagaag gagagccggt accgctgtga tattcatcac attttaaaaa
     1261 taaaaggaac gtataaagga aaaaaatgaa acttgttata tacagagtat aacaagtgtg
     1321 ttatattgag tatataacaa acggagaggt tgtgatgttc atgtcagatc ttgcaaacat
     1381 accttggagt cttgttttac ctatcgttgt acttcaactg atcctgatgg ttgtcgcttt
     1441 ggtcgatctc attcgtcatc aacggacgaa cgggccattc atcatgtgga ttttcatcat
     1501 attactgggc aatatcattg gttcgatcct gtacttcatc ttcgggagga aacaagcatg
     1561 acccaattgc tgcaagtgaa tgatctatca aagatttacg ggagtcataa agcagcggat
     1621 tcgatcacat tcgaattggc ggaaggcaca tcgacggcat tgatcgggcc gaacggggca
     1681 gggaagacaa cgacattgtc catgctcgcg ggacttgtca cgccgacgag tggatcgatt
     1741 cttttccggg gtaaaccgaa gccggatatc cgggcttcca tcggctatct tcctcaatac
     1801 ccgaagttct ttccttggct gacagcgctt gagttcacgg aaatggctgc tgtattgagc
     1861 ggtgtcgaaa ctaggcaggc attggcggaa gcgaaaaaga cgctggagtt cgtcggcctt
     1921 ggggatgcga tgaagaagcg gacgggaacg ctttccgggg ggatgaagca gaggctggga
     1981 ttggcgcagg cgattgtcca tggaccggag ctgttgctat tggatgaacc tgtatcggca
     2041 cttgacccgg tcggacgacg gcaagtgatg aacctattga aggaacttca acagaaaacg
     2101 acaattctct attcaactca tattttgaat gatgcggaag agatgacaga ccaactatta
     2161 ttcttgcgca aaggcgtcct tgtcgaaaat ggatcgctgg agacaatccg tgagaaatac
     2221 gccaaccctc gtattctcat cgaattcgat tctgtggaag aggcggacag atttaccgca
     2281 ctttccccat gggcagtcaa gatggagaag aagcaagttt ctatcgatgc tgtcaaggaa
     2341 ggggtcggca cgaatgaagt aatcgagcag ctacatacgg agtcactcca aatccggaag
     2401 gttgagctgc agactgcaag tttggaagaa atcttcatga aagtggtgat ggagggatga
     2461 gcagcctcaa tgtactgttg cgcaaggaat ggcgtgaaaa tattcgtaac tttaaacttt
     2521 actggattcc aatcgtgttt atcatctttg gggtgattga acctatcacg aaccactttc
     2581 ttcctgaaat tatgaaaagt gtaggaaacc tgccggaagg gacggaattc gcatggcctg
     2641 aattccgagg ggaagacatt ttcgtctcgt tgatgagtca ataccagatg attggcatcc
     2701 ttattatcat cctcgctttt atgggatcca tttcaggaga gcggaaatca ggaacagcaa
     2761 cgcttttata cgtgcgacct atgtcatttg gccactattt ctttagcaag tggattgtcg
     2821 tcaatgcgat tgtattggca agtgtttggt taggtttttt tgcggcatgg tattatatcg
     2881 ggattttatt caactcagtg gcggcagggg aagtcttcgc gtttgtaggg atttatagtt
     2941 tgtggttggt ttttgctgtg actgttgtcc ttgcgttaag cgcgtggttg ccaacaggag
     3001 cggttgcagg gctttccatc ttagtcttga tcgttttcca aattatagac ggtgtagtcg
     3061 gcatgtactg gacagtgaca ccatggaaat taagctcata tgcaccgaat gcattcagca
     3121 cgttcgccga tcaatcagcc ttttggatga gtgtcgggct tacggtcgcc ctcattattg
     3181 cgcttgtctt tttcggtata tacatgacga aacggaatgc tgccaagaca ttggtctgac
     3241 atttcccggg taatatcgac aggtgacgac acggatagtt gcaaaaagta gaggattgga
     3301 attgaattcc acctctactt ttttgcgctt atttatattt ctcctcactc gacgaactgc
     3361 ttttcgtttc acgatgatgc ttttcctcgt gttcttccaa tcttacatct tccaatggaa
     3421 tcggatcgac gttttgcatg tcttcgtgca tatcatagat gctttccttg tcagtcggca
     3481 agttttcggg attgtccatg aaaggacctt cggcgtcttt ccgatcttct gaagtaaaag
     3541 gttttttctt attcaatgat agcacttcct ttctgttctt attctattca cgcgatccgc
     3601 ttgggctaaa catggcacac aagtgattga aatgtgtttt cagtcgattc taggggtaaa
     3661 gtaatgaaaa caaatgaaaa aggagtgttt cgacaaatgg aaaaaaggta tgtcggcacg
     3721 caaaataaaa ttgacgcggc tttgtataaa atcacggaat tgacatcaca gggctattcg
     3781 gaggaccaga tttccgctgt ggcaaatact gatgatgact ttgcacaatt gcggaggcaa
     3841 acgtttgtat ctttggaggg gcaaggaaag gaaggctttc ttgatcgttt taaagggaca
     3901 tttctcgatg agcagccgaa acaggctaga tactttgaag agatgggctt ttcaagggag
     3961 gaagccgatc aaatctataa tgatatgaag gatggcggaa ttgcaatatt tgtcgataat
     4021 gtcgaaccgg atattgcaat cgaaggacgc ggaggcggag aagttgaaga cacggaaagg
     4081 gatagcaggg cgcaattgac ggatagcggt gaaatgttgg cgggtgacga cgacatcttg
     4141 cagcctagag ataccaatgc aggaacggtc ccgcgaatca acaccgacca tttatgacaa
     4201 ggaaaggctg cctcccttac atgtaggaga cagcctgccc ttcagatgaa attagcgacg
     4261 ctgattcttg tgattgccga atcggtcctt gaattcaccg atttcctgct gcgcttcgcc
     4321 tttcaattta tccattttgc cttccgcctg caacattgca tcatccgttg cattgccata
     4381 ttgatctttt gcttcacctt tgactttatt gacggcacct tttaccttat ccgagtaacc
     4441 tctttctttc atcatgaaaa cctccttaaa gagtagtagt ttttcaatac cctgcaagga
     4501 aaaaaggcaa acgtacagac cgggacagct tgaaatttga aaaaagcaag agtggaaagt
     4561 ccttgaaaat tatgtcgaaa ttttggttga acttaccttt tcccatattt gtccgcttta
     4621 tataatggat gaatacatat gcaattcttg gaggagatgg catgataaga aaaggtcgtt
     4681 ttgcagtttg gaacggtaaa gaatatccgc ttctttcaca acgacgtaaa tattatttgc
     4741 aatccgtcaa tccggaagat aaggaaattg ggtttgtcga aatgggtacg agggggtatt
     4801 tcttcaagga aatcagcacg gatgatttgg aagatgctta cgaggtattt ccctatgctc
     4861 tactatccgg ctatcgtttt gcattggaga gttatgaaaa ggagacccgc atcgtaacat
     4921 tcgtgacgaa caaccctttt gcccaaaaga aagtccctgt cagtctacac ggaaatgaat
     4981 tccggatcga cattccgatg gaccaagtcg aaattctgga ggaccgcatt cccattttgg
     5041 gcttcgaatc aattaataga tgaaggaatg cattacattc cttcatccgc tatttttttc
     5101 acattcacaa gttcatataa aatatcatcc gattcggaag gggttccccg cccgtccgta
     5161 ttattggaaa tgaagtaaag agactctccg tctgaataga cgtcccgaat acggccgtaa
     5221 ccttcgattc ttcttgtaat ctccatcgtt tccgggtcga tcaccaaaat ggctttccct
     5281 ctgagcgccg caacatagag catcccttta tgaaacgtca tcccggatgg cgcccacgtc
     5341 gcgcttgctc ctgaagtgag cagtggggaa agcatgcctt ccttgctttc attcccttca
     5401 atttcaggcc agccataatt cccaccctgc acaatctcat tgatttcatc atttgcggac
     5461 tgtccatgct ccgaagaata gagcttacct ttcgaattcc aagcaagtcc ttgagggttc
     5521 cgatgccccg aactgacaat ttcaaagctg ccatcctctt ggagc
//
LOCUS       DASULI010000377         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1023056,
            whole genome shotgun sequence.
ACCESSION   DASULI010000377 DASULI010000000
VERSION     DASULI010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023056"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="ccsA"
                     /locus_tag="VFX34_08145"
     CDS             complement(<1..537)
                     /gene="ccsA"
                     /locus_tag="VFX34_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5564916.1"
                     /translation="MAELTMARFHEVIVVLYAVSLVFYFIDYLNNNKLAHRSAFGILS
                     VVYLMQSGLLIARVIDTQRFPVLSLYEGIYFYAWLLISLSMILQVFSKVGFAVFFLNV
                     IGFIFMTIHTFTPVQFEKSPVGESLISELLFIHITFAILSYAAFAMSFVFAILYLLVY
                     KVLKKKKWTKQFGRLPSLQ"
     gene            complement(578..1918)
                     /gene="hemA"
                     /locus_tag="VFX34_08150"
     CDS             complement(578..1918)
                     /gene="hemA"
                     /locus_tag="VFX34_08150"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX5564917.1"
                     /translation="MYTIVVGVNHRTAPVEIREKLSFVEAELPKAMQVLQKEKSILEN
                     VIISTCNRTEIYAVADQIHTGRYYVKRFLADWFDMELEELSPHLLIYENDAAIEHLMR
                     VSAGIDSMVLGETQILGQVRSSFLEAQDIGTTGTVFNELFKKSITLARRAHSETAIAE
                     NAVSVSYAAVELGKKIFGSLNGKHIAILGAGKMGELAIKNLHGSGAGKVTVVNRTLSK
                     AEEMAEQFGGVAKPMQELQCTLLEADVLISSTGATDFVIDFELMQYVEKLRKGKPIFM
                     VDIAVPRDLDPRIGDLPNVFLYDIDDLQGIVEANLAERKRAAEEIGLMIEEEIVEFKE
                     WIATLGVVPVISALREKALNIQAETMMSIENKMPNLTAREKKLLNKHTKSIINQMLKE
                     PILQAKELALDDQAAEKLTLFKQIFGLAEDKEAEVAVQAEADSVGNPTRKLAEA"
BASE COUNT          551 a          522 c          430 g          600 t
ORIGIN      
        1 ttggagagaa ggaagcctcc cgaattgctt tgtccatttt ttctttttca gtactttgta
       61 gacgagcaaa tataggatgg cgaagacgaa ggacatggca aatgccgcat acgaaagaat
      121 tgcaaatgtg atatgtatga agagcaattc cgaaataagt gattcaccga ccggggattt
      181 ctcgaactgc accggtgtga acgtatggat tgtcatgaaa atgaatccga tcacatttaa
      241 aaagaagaca gcgaacccga cctttgaaaa cacttgaagg atcatcgata acgaaatgag
      301 cagccaagcg tagaaataga tcccttcgta aagggaaagg accgggaatc gctgtgtgtc
      361 aatcacccgc gcaatcagga gtccgctttg catcaaatag acgaccgaca aaatcccgaa
      421 agcgcttcgg tgggcgagtt tattattatt caaataatca atgaagtaaa atacgaggct
      481 caccgcatac aggacgacaa ttacttcgtg gaatctcgcc attgtcaatt cggccatgat
      541 gcaacctctg cctttcacat gaatctatac tctcggatta cgcctcagct aatttacgcg
      601 ttggattgcc gacggaatcg gcttccgcct ggacggccac ttcagcttct ttgtcttcag
      661 ccaatccgaa tatctgcttg aataaagtga gtttttcagc tgcctgatca tccaatgcca
      721 actccttggc ttgtaggatc ggctccttca gcatttggtt gatgatcgac ttcgtatgct
      781 tattcagcaa tttcttttca cgcgccgtca agttcggcat tttattttct atgctcatca
      841 ttgtttccgc ttggatgttc agcgcctttt cccgcaatgc agaaatgacc ggaacgacgc
      901 caagtgttgc gatccattct ttgaattcga cgatttcctc ttcaatcatt aaaccgatct
      961 cttcagcagc acgtttacgc tccgccaagt tcgcttcgac aattccttgc aagtcgtcaa
     1021 tatcatatag gaatacgttc ggcaaatcac cgattcgcgg atccaaatcc cgtggtacgg
     1081 caatatcgac catgaaaatc ggcttccctt tacgaagttt ctcaacatat tgcatcaatt
     1141 cgaaatcaat gacaaagtca gttgcgcctg tagagctgat taaaacatcc gcctctaata
     1201 atgtgcattg caattcctgc atcggttttg cgacgcctcc gaattgctcc gccatctctt
     1261 ctgccttcga tagtgtacga ttgacgactg tcactttgcc ggctccgctg ccatgcaaat
     1321 tcttgatcgc caattctccc atcttcccag ctccaaggat ggcaatatgc tttccattca
     1381 gtgaaccgaa gattttcttg ccaagctcaa cggcagcata ggacacggaa acagcatttt
     1441 ccgcgatggc agtttctgaa tgtgcccgtc ttgcgagtgt aatcgacttt ttgaacaatt
     1501 cattgaaaac tgtccccgtc gtcccgatat cctgagcttc caggaagctg ctgcggactt
     1561 gtcctaaaat ttgcgtttcg ccaagcacca ttgagtcgat acccgctgaa acacgcatca
     1621 aatgttcaat tgctgcatca ttttcataaa tgagcaagtg tggggataat tcctcaagct
     1681 ccatatcgaa ccagtccgct aaaaatcgtt tgacataata acggcccgta tggatttgat
     1741 ctgccacagc atatatttcg gtacgattgc aggtagaaat gatgacgttc tccaaaatgc
     1801 ttttctcttt ttgcaaaacc tgcatcgcct tcggcagttc agcttctacg aaagataatt
     1861 tttccctgat ttccactgga gcagttcggt ggttgacacc gaccacgatt gtatacataa
     1921 ggtgttcaca cctctcccgt tctttttcac atatacgatt ataacatatt acgctgtatc
     1981 taacggctaa ttttgtgact aactcttgaa ccatcaatgg ttcgttccct gtttttgtaa
     2041 ttattctaat gtaacagcat ccagctccgg cggccagatg ctcggggtca taagtcggcc
     2101 ctg
//
LOCUS       DASULI010000378         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_711712, whole
            genome shotgun sequence.
ACCESSION   DASULI010000378 DASULI010000000
VERSION     DASULI010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711712"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..564)
                     /locus_tag="VFX34_08155"
     CDS             complement(88..564)
                     /locus_tag="VFX34_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5564918.1"
                     /translation="MKKLLIIGGVIVAIFALIIVLNNQSNKEKLKDNPYGTDQLEQAT
                     IDLLGNENYSNIVLPDELFEKVSSGESVTAYFFSPECPYCMEMTPILMPIAKEKGIHV
                     YQYNMLEFQDQAQPYGITSWPTLIHYEDGKEVSRSVGLPPNPEEDIRAFFDEYDKK"
     gene            complement(566..991)
                     /locus_tag="VFX34_08160"
     CDS             complement(566..991)
                     /locus_tag="VFX34_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HEX5564919.1"
                     /translation="MHKRLENGLVFIFTISLIATFGSLYFSIIRGYEPCTLCWYQRIL
                     MYPIVLMTGVALFQKNAKIALTTAVFSIVGGSISLYHYGLQKLRFLQDSAPACGNVSC
                     TGQYVNYLGFITIPFMALTAFVLILITSLFLLKWQKESN"
     gene            1123..>2044
                     /locus_tag="VFX34_08165"
     CDS             1123..>2044
                     /locus_tag="VFX34_08165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5564920.1"
                     /translation="MSEEIDLSSFEMSMIIREMKEDDIEKILNMQEVCFPGMEPWEEE
                     HLKSHLSIFPEGQFVAELDGEIIGSCSSLIINFDEYDDRHSWDDISAKGYITNHNPEG
                     YNLYGIEVMVHPEYRRMKVGQRLYEARKELVQRLNLKSIIIGGRIPNYHRYADEMSAR
                     EYVEAVTRHKIYDPVLTFQLLNDFTLMRINPNYLPDDKASKRYATLMEWNNVDYKATT
                     KRHYKTSYPVRICVVQYLMRKISSFEEMANQCEYFVDVASDANSDFVVFPEIFTTQLM
                     SYLDEPSPSRAVRKMTEYTPQYIEMFTNLAV"
BASE COUNT          573 a          447 c          492 g          532 t
ORIGIN      
        1 cggctttcca atcatcccga aggagctgct caatcgtcaa tccatcccgg atcatccgat
       61 aacggaaggc tgttttctgc ttatctgtca ttttttatcg tactcatcga agaaggcccg
      121 gatgtcttcc tcgggatttg gcggcaagcc gacggagcgg gaaacttctt ttccatcctc
      181 gtaatggatg agcgtcggcc atgatgtgat gccataaggc tgcgcttgat cttggaactc
      241 caacatattg tattgataca cgtgaatccc tttctctttt gcaatcggca tgagaatcgg
      301 cgtcatctcc atgcagtacg ggcattccgg agagaagaag taagctgtga cagactcacc
      361 ggaagagacc ttttcaaaga gttcatccgg caggacgata ttgctgtaat tttcatttcc
      421 aagcaagtcg atcgtcgctt gttcaagttg gtccgttcca tacggattgt ccttcaattt
      481 ttctttgttt gattgattat tcaatacgat gattaaagca aagatggcga cgatgacacc
      541 gccgataatc agtaatttct tcaaattagt tagactcctt ttgccatttt agtaagaaga
      601 ggcttgtaat gaggatcagc acgaatgccg tcaaagccat gaacggaata gtgatgaaac
      661 cgaggtaatt cacatactgg cccgtacagg aaacattccc gcaggccggt gcgctgtcct
      721 gcagaaaacg cagtttctgg agtccataat ggtatagtga aatggaaccc ccgacaatcg
      781 aaaagacggc tgttgtcaat gcgatcttag cgtttttctg gaataaagcg acacctgtca
      841 tcaaaacaat cggatacata aggattcgtt gataccagca tagagtgcaa ggttcataac
      901 cccttatgat ggaaaaatac aatgacccga atgttgcgat gagggaaatg gtgaaaatga
      961 aaacgaggcc gttctccaac cgtttgtgca tactgtcaac tcctgtttct acgactccac
     1021 tttgattata cggtgtgggc gtattcatgt aaaatattct aaaccacatg acttttccta
     1081 gataattatg ggtacagatt atgtagaaag ggtgaacttt ggatgagtga agaaattgat
     1141 ttatcatcat ttgaaatgag catgatcatc cgcgagatga aagaggatga catcgaaaag
     1201 atattgaaca tgcaagaggt gtgtttccca ggcatggagc catgggagga ggaacatctg
     1261 aaaagccatc tgtccatctt tcctgaaggg cagttcgtcg ccgagctcga tggggaaatc
     1321 atcggttcct gctcaagcct gatcatcaat tttgatgaat atgatgaccg ccattcctgg
     1381 gacgatattt ccgcgaaggg ctacattacg aaccataatc ctgaaggata caatctgtat
     1441 ggcatcgaag tgatggtcca tcccgaatac cggaggatga aagtcgggca acggctgtat
     1501 gaggcaagga aagagcttgt ccaacggctg aatttgaaat cgatcatcat cggcggcaga
     1561 atcccgaact accatcggta tgcggatgaa atgtcggcac gcgaatatgt agaggcagtg
     1621 acccggcata aaatctatga tcccgttctc acgttccagc tcctgaatga tttcacgctc
     1681 atgcggatca atccgaacta cttgcccgac gacaaagcgt caaaacgata tgcgacgctg
     1741 atggaatgga ataatgtcga ttataaggcg acgacaaaac ggcattataa gacgagctat
     1801 cctgtccgta tttgcgtcgt ccaatatttg atgaggaaaa tatcgtcgtt cgaggaaatg
     1861 gcgaaccaat gcgaatactt tgtggatgtt gcgtcggatg caaattcgga tttcgtcgtc
     1921 ttccctgaaa tctttacgac gcagctcatg tcctacttgg atgagccttc gccgagcagg
     1981 gcagtgcgca agatgacgga atacacgccg cagtacattg aaatgttcac gaatcttgcc
     2041 gtcc
//
LOCUS       DASULI010000379         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_692598, whole
            genome shotgun sequence.
ACCESSION   DASULI010000379 DASULI010000000
VERSION     DASULI010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692598"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VFX34_08170"
     CDS             complement(<1..161)
                     /locus_tag="VFX34_08170"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015729078.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP; lysine and threonine sensitive;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX5564921.1"
                     /translation="MKVCKFGGTSVATAEQIRKVVDIVTSDSDRKIVVVSAPGKRFSD
                     DIKVTDLLIR"
     gene            319..1617
                     /locus_tag="VFX34_08175"
     CDS             319..1617
                     /locus_tag="VFX34_08175"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5564922.1"
                     /translation="MIKEINIGLLGFGVVGGGVAEILHRHQEDLSHKLGVPVKIKKVL
                     VKDAAKKRETELSPDIFTTDLDEVIENPGIDLIVEVIGGTSEAKQAIEKALRAGKGVV
                     TANKDVMAQFGHDLLKLADEHKCDLFYEASVGGGIPLIRTLEDGLASDRIRALTGIVN
                     GTTNFILTKMKHENKTYEEALAEATELGYAEADPSADVDGIDAARKMAILASLAFSTE
                     VRLEDVFVRGMKDIQDGDLALAEQFGFTVKMAGFAKKDDDGIEISVEPVFIQNSHPLA
                     SVNNEFNAVYVYGDTVGETMFYGPGAGSLPTATSVTGDIVAACRNLLLGVNGKRLHAP
                     QFAYKVKDDNQIIGRYFHRISVKDEVGVLTKLASVYSNHGASLATVVQHEGDNNGGVD
                     LIMITHSISRQQHLDIMNELHNTPEVNGVVSYYRVEGENR"
     gene            1614..2675
                     /gene="thrC"
                     /locus_tag="VFX34_08180"
     CDS             1614..2675
                     /gene="thrC"
                     /locus_tag="VFX34_08180"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX5564923.1"
                     /translation="MRRWNGLIEEYKEWLPVTDKTPELTLQEGNTPLIHLKNLSEQWG
                     INLYVKTEGTNPTGSFKDRGMVMAVAKAKEEGKTALICASTGNTSASAAAYGARAGMR
                     TIVVIPEGRIALGKLAQAKMYGAEIVAIEGNFDEALRMVREAGEGKIALVNSVNPYRL
                     EGQKTIAFETIEQLGKVPDIFALPVGNAGNISAAWKGFKEYDAKKGSGTPKLLGVQAD
                     GAAPIVYDRVFEEPETVATAIRIGNPASWHLATAALEESGGDILSATDEEILEAYSLL
                     ASTDGVFAEPASCATIAGIKKRLDAGLIEKGTTIVGVLTGNGLKDPETAIHVNEGKAF
                     LSREQFDAFLNKLKEGATS"
     gene            2675..>3438
                     /gene="thrB"
                     /locus_tag="VFX34_08185"
     CDS             2675..>3438
                     /gene="thrB"
                     /locus_tag="VFX34_08185"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX5564924.1"
                     /translation="MERPLFTVVVPATTANLGPGYDSVGMALSLYMTVSVTPADNWSV
                     QYEGDEYAKLTTGPNNLIIRTVLNVAERFGKEVAPHSLHVVSDIPLGKGLGSSATAIA
                     AGIVIADGLLGLDLQPVEKAQIGSELEGHSDNVTAALLGGITITYFTQDELEIIQLPR
                     PPIGAVVLVPPKALRTEESRGMLPNQLSHPEAAAGSAASSTMTTAMAIGNWELAGRMM
                     EKDVFHEPYRKKLLPEFDEIRSFCHEIGAYGMTISGA"
BASE COUNT          940 a          778 c          974 g          746 t
ORIGIN      
        1 cggatgagga ggtcggtcac tttaatgtca tctgaaaagc gttttccggg tgctgaaact
       61 acaacgattt tacggtcact gtcagaagtt acaatgtcga ctacttttct aatttgctcg
      121 gctgtcgcaa cagatgtgcc accgaattta catactttca tatttatcaa atccattctt
      181 taataatatt acaattcatg atatcttgaa ctttaacata aatggggttg ttattgaaag
      241 tttatttctc tataatgtcc ttatgcgaag aacgattgtt ttttcatagt gtacatatag
      301 cgaggagaat cgtcaaatat gattaaagaa atcaatatcg gtttgcttgg attcggtgtc
      361 gtaggcggcg gtgtagctga aatcctgcat aggcatcaag aagatttaag ccataaattg
      421 ggcgttcctg taaaaataaa aaaagttctc gtgaaagatg cggcaaagaa aagggagaca
      481 gaactgtcac cggatatttt cactacagat ctggatgaag tgattgaaaa tccgggaatc
      541 gatctcatcg tggaagtgat cggcgggaca agcgaggcga aacaggcgat cgagaaggcg
      601 cttcgcgcgg gcaaaggggt tgtgactgca aataaggacg tcatggcaca gttcggccat
      661 gacctcttaa agcttgcgga tgaacataaa tgcgacctgt tctatgaagc gagcgtcggg
      721 ggcggcatcc cgctcatccg gacgcttgaa gacggactcg catctgaccg gatccgggcg
      781 ctgacaggca tcgtcaatgg aacgacgaac ttcattttga cgaaaatgaa acacgaaaac
      841 aaaacgtatg aagaggcact cgcggaagcg accgagctcg gatatgcgga agccgaccct
      901 tcagcagatg tcgacggcat cgatgccgcc aggaaaatgg cgatcctcgc atcgctcgca
      961 ttttcaacgg aagtccgctt ggaggatgtc tttgtaagag gcatgaagga catccaggac
     1021 ggcgacctcg cattggcgga gcagttcggc ttcaccgtga aaatggcagg attcgcgaaa
     1081 aaagatgacg acggcatcga aatctcagta gaaccggtgt tcatccaaaa ttcgcatccg
     1141 cttgcctctg tcaataatga atttaatgct gtctatgtat atggcgatac ggtcggagag
     1201 acgatgttct acggaccggg agcgggttca ttgccgacag cgacttccgt tacaggagac
     1261 attgtcgcag catgccgtaa tttattgcta ggcgtaaacg gaaaaaggct tcacgcgccg
     1321 caatttgcat acaaagtgaa agacgacaac caaatcatcg gccgctactt ccaccggatc
     1381 tccgtaaagg acgaagtggg cgtattgacg aaattggcat cggtctatag taatcatggg
     1441 gcaagcctag ctaccgtcgt ccagcatgaa ggcgacaaca acggcggcgt cgacttgatc
     1501 atgatcacac atagcatttc aaggcaacag catttggaca tcatgaatga actgcataac
     1561 acaccggaag tgaacggcgt tgttagctac taccgagtgg agggcgaaaa cagatgagaa
     1621 gatggaacgg attgatcgaa gaatacaaag aatggctgcc ggttaccgac aaaacaccag
     1681 aactgacatt gcaagaaggg aatacaccgc tcatccactt gaaaaacctt tccgaacaat
     1741 ggggtatcaa cctatacgtc aaaacggaag ggacaaaccc gacaggctct ttcaaggatc
     1801 gcggcatggt gatggccgtc gctaaagcga aagaggaagg aaaaaccgcg ctcatctgcg
     1861 cttcaacagg caatacgtcc gcttctgcgg cggcatatgg cgcacgcgct gggatgcgga
     1921 ccatcgtcgt catcccggaa ggacggatcg cgctcgggaa attggcgcag gcaaaaatgt
     1981 acggagctga aatcgtggcg atcgaaggga atttcgacga agcgctgcgc atggtgcgtg
     2041 aagcggggga agggaaaatc gccctcgtca attcagtgaa cccttaccgg ttggaaggcc
     2101 aaaagacaat cgctttcgag acaattgaac aattagggaa agtgccggat atttttgcgt
     2161 tgccggtcgg gaatgcaggg aatatttccg ctgcatggaa aggtttcaag gaatatgacg
     2221 cgaaaaaagg gtcgggcacg ccgaagctgt taggcgtaca ggcggacgga gcggctccga
     2281 tcgtttatga ccgcgtattt gaagagcctg aaacggtcgc gacagccatc cggatcggga
     2341 atcccgcaag ctggcatttg gcaacggcgg cgcttgaaga gtctggtggc gacatcctgt
     2401 ctgcaacgga cgaggagatt ttggaagctt atagtttgct cgcctctacg gacggcgtct
     2461 ttgcggagcc ggcttcatgc gccacgatcg caggcataaa gaagcgactc gacgcggggt
     2521 tgattgaaaa aggaacgacg attgtcggcg tacttacggg taatggattg aaggatcccg
     2581 aaacagccat ccatgtgaat gaggggaaag ccttcttgtc gagggagcag tttgacgcgt
     2641 ttttgaataa attaaaagaa ggggccacga gctgatggaa aggccattat tcacggtcgt
     2701 cgtcccagcc acgacggcga acctgggacc ggggtatgac agcgtcggca tggcgctttc
     2761 cctgtatatg accgtatcgg tgaccccagc tgataattgg tctgtacagt atgaagggga
     2821 cgagtatgca aaattgacga cgggaccgaa taatttgatt atccggaccg tcctgaatgt
     2881 ggcggagcgt ttcggaaaag aagtagcccc gcattccttg catgtcgttt ctgacattcc
     2941 tcttggcaaa gggctcggca gcagcgcaac agcgattgct gcgggtatcg tcatagcgga
     3001 cgggcttctc ggactcgatt tgcagccagt cgagaaggca cagatcggga gtgaactgga
     3061 aggccattcg gataatgtga ccgcggcatt gctcggtggc ataacaatta cgtatttcac
     3121 tcaggatgaa ttggaaatca tccaattgcc ccgaccgccg attggtgcgg tcgttctcgt
     3181 gcctccgaag gcgctccgta cggaggagtc gagaggtatg ctcccgaatc agctgtccca
     3241 tccggaagcg gccgcaggaa gcgcggccag cagcacgatg actacggcaa tggccatcgg
     3301 aaactgggag ctggcaggca ggatgatgga aaaggatgtc ttccatgagc cgtaccgtaa
     3361 aaaactgctg cctgaatttg atgaaattcg ttccttttgc catgaaattg gcgcgtacgg
     3421 catgacaatc agcggcgc
//
LOCUS       DASULI010000380         7285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1942146,
            whole genome shotgun sequence.
ACCESSION   DASULI010000380 DASULI010000000
VERSION     DASULI010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942146"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="VFX34_08190"
     CDS             <1..1518
                     /locus_tag="VFX34_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX5564925.1"
                     /translation="LTLYITYWAAKQTRTASEFYTAGGSLTGWQNGMAIAGDYLSAAS
                     FLGIAGAISLNGFDGFYYSVGWLTAYLVVLFLIAEPLRNLGKYTMADMIGSRFGAKKV
                     RGAAALNTITIVLFYMLAQLVGAGALIKLLFGIDYWISVLIVGFMMLVYVLFGGMTAT
                     SWVQIIKAILLMVGTIVISFLVLMKFNFNFVGMFDLMRTATPHGESFLHSGVVYKDPI
                     GLISVLLALVLGTAGLPHILMRFFTVKDAKTARSSVVYAVWIIGIFYVMTIFLGFGAA
                     KFVGRDAIVAENSAGNMAAPMLAGVLGGDPLESFVAAVAFATILAVVAGLVLSGASAI
                     AHDIYAEIIKKGKVTEKQQVRAARLAAIGVSVVSILLALGSEKLNVAFLVSLAFCIAA
                     SANVPTILFTIYWRKFNTTGAVASMLTGLITALILVAVSPNIINPVEGAAFFVGEPLF
                     PFVNPAIISIPAGFIAAIVGTYVGAKRNEVDEIAYAEVRFKAETGFRGLEGQTPN"
     gene            complement(1592..3007)
                     /gene="aspA"
                     /locus_tag="VFX34_08195"
     CDS             complement(1592..3007)
                     /gene="aspA"
                     /locus_tag="VFX34_08195"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX5564926.1"
                     /translation="MNLLSGRIEKDFLGEKEVPDHAYYGIQTIRATENFPITKERMHP
                     ELIRAIGIVKKAAAISNVLVNDLPEDVGLAIVGAADEVIKGRLTDQFIVDPIQGGAGT
                     SFNMNANEVIANRALEMMGREKGDYGFISPNNHVNMSQSTNDAFPTAIKIATQQMVNQ
                     LLETMKKFVDELKAKEKEFDGVIKMGRTHLQDAVPVRLGQEFSAYRSVIERDIERIGR
                     TRVHLSEINMGATAIGTGLNANAQYIEIAVEHLRTISGLPLKSAGNLIDSTQNTDVYT
                     ELSSALKICMINLSKMANDLRLMASGPKAGLNEINLPARQPGSSIMPGKVNPVMAEVM
                     NQIAFQVIGNDHTISLASEAGQFELNVMEPVLVYNLLQSLTVMNNGIEVFSKYCISGI
                     TANVSQMQEYVERSIGTITALTPFIGYEMSSKIARESFETGVPLKELCLRYNVLTLDE
                     LTRLLDPYQMTEMKREGVAHY"
     gene            complement(3177..3650)
                     /locus_tag="VFX34_08200"
     CDS             complement(3177..3650)
                     /locus_tag="VFX34_08200"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEX5564927.1"
                     /translation="MSTTEKKMNVESFNLDHTKVKAPFVRLAGVKEGANGDVIRKYDI
                     RFCQPNQEHLEMPAIHSLEHMMAEFSRNHSDRIVDISPMGCQTGYYLALINHDDYEDV
                     LDILEKTLNDVLAADEVPACNEVQCGWAASHSLEGAKEAAKKMLGKRAEWTTVFE"
     gene            3837..4787
                     /locus_tag="VFX34_08205"
     CDS             3837..4787
                     /locus_tag="VFX34_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564928.1"
                     /translation="MIRFDGVTKSYDNQTLVVDSFNLEVAKGEFLVLIGPSGCGKTTT
                     LKMINRLIPLTDGTIWIDGRKVSEYAIHELRWRIGYVLQQIALFPHMTIEENIAIVPE
                     LKNWNKSRIHHRVTELLEMVGLDPDTYRHRKPHELSGGQQQRVGVVRALAADPDIILM
                     DEPFSALDPISRGKLQEDLLQLQRSIQKTIVFVTHDIQEAMKLGDRICIMKDGKIEQL
                     GTPDEIMLTPATPFVRKFIQSGSQKPFVLSEHIEPMTTDTERLPVLSVNAGWDEVLHA
                     LSLSDRVLIQQEGAPIGTISRQTIIAYLASGQEGGKIAHG"
     gene            4780..6297
                     /gene="opuFB"
                     /locus_tag="VFX34_08210"
     CDS             4780..6297
                     /gene="opuFB"
                     /locus_tag="VFX34_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008175684.1"
                     /note="The ABC transporter OpuF is widely distributed in
                     Bacillus species other than B. subtilis. OpuFA is the
                     ATP-binding subunit, while OpuFB is a fusion of permease
                     and substrate-binding subunits; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant update ABC transporter
                     permease/substrate-binding subunit OpuFB"
                     /protein_id="HEX5564929.1"
                     /translation="MGEFIGVFQDRKGELAWALMEHIQISLIALFFAVLISIPVGVYL
                     TSRRRIAETVIGTSAVLQTIPSLALLGLLIPLFGIGKVPAIIALVAYALLPILRNTYT
                     GIKGIDPSLIEAATALGMNRRKRLTKVELPLAMPVIMAGIRTSMVLIVGTATLAALIG
                     AGGLGDLILLGIDRNSPSLIVLGAIPAALLALSFDLLLRRFEGLSFRQASMALTAFSV
                     AAVLIISLPLFPASNERQLVIGAKLGAEPEILINMYKLLIEEETDLSVELKPGLGKTS
                     FVFNALQSGSIDIYPEFTGTALSEFLKEAALSNDRNEVYEQARVGLAEQFDLVLLPPM
                     EYNNTYAIAVSKAFEEQYGVKTISDLFPVQTAVKAGFTLEFNDREDGYRGIRSLYGID
                     FPSVQTMEPKLRYQAIRSGDINLLDAYSTDSELQQYELTVLQDDKELFPPYQGAPLLR
                     KETLETYPELEEILNRLAGKVTDDEMRDMNYRVNAEGEKPEDVARQFLQEAGLLE"
     gene            complement(6340..7014)
                     /locus_tag="VFX34_08215"
     CDS             complement(6340..7014)
                     /locus_tag="VFX34_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018782840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX5564930.1"
                     /translation="MKKQWNHKGIIEKFEMCRNVEQAGPMKAYMKHHFPFLGIKSPLR
                     KELLREHFREYALPEAGEAFAEAWKLYELPEREYQYAAIALLEKMKKHFTTDDYPKLL
                     KFIETKSWWDSVDSIAPHLVGHVVKLDRDYRKTVMLEWSQSENMWTNRSAILHQLKFK
                     KETDTELLFEIILQHVESNEFFIQKAIGWVLREYAKTDPVIVKQFVEDHSLKPLSRRE
                     ALKHIG"
BASE COUNT         1738 a         1924 c         1729 g         1894 t
ORIGIN      
        1 ttaacgttat acatcactta ttgggctgct aaacagacgc gcacggcgag tgagttctat
       61 acggccggcg gatcgttgac agggtggcaa aacgggatgg ccattgcggg agactatttg
      121 tccgccgcct ctttccttgg aattgcagga gccatttcat tgaacggatt tgacggtttc
      181 tattacagcg tcggctggtt gaccgcatat ctcgtcgtcc tgtttttaat cgcggaaccg
      241 cttcggaatc tcggcaagta cacgatggcc gatatgatcg gttcacggtt cggagcgaag
      301 aaagtccgcg gagctgctgc gcttaatacg attactatcg tccttttcta tatgctcgca
      361 cagctagtcg gcgcaggcgc gctcatcaag ctgctcttcg gaatcgacta ttggatttcc
      421 gtcctgatcg tcggcttcat gatgttggtt tatgttttat tcggcggaat gactgctacg
      481 agctgggtgc agatcatcaa ggctatcctg cttatggtcg gaacaatcgt catttcattc
      541 ctcgtcctca tgaaattcaa tttcaatttc gtcggcatgt tcgatctcat gcggacagcg
      601 acgccgcacg gagagtcatt cctgcattcc ggcgttgttt ataaagatcc gatcggactc
      661 atttcggtgc tcctcgcgct cgttcttgga actgcgggat tgccgcacat cctcatgcgt
      721 ttctttacag taaaagacgc aaaaacggcg cgttcttccg tcgtatacgc agtgtggatc
      781 atcgggattt tctacgtcat gacgattttc ctcggattcg gtgcagccaa attcgtcggc
      841 agggacgcca ttgtcgcgga aaactccgcg ggcaacatgg ccgctccaat gctagctggt
      901 gtgctcggcg gggatccgct tgagtcattc gttgcggctg tagcattcgc aacgattctt
      961 gcagtcgtag cagggctcgt gctctccgga gcatctgcaa ttgctcatga tatttacgcg
     1021 gaaatcatca aaaaaggcaa agtgacggaa aagcagcaag tacgggcagc ccgtcttgca
     1081 gcgatcgggg tatccgtcgt ttccattttg cttgcactag gttcggaaaa gctgaatgtc
     1141 gcgttcctcg tgtcgctcgc attctgtatc gcggcatccg cgaacgtacc gacaatcctg
     1201 tttacaatct attggcgtaa attcaatacg acaggcgctg tcgcttcaat gctgacaggg
     1261 ctcatcacgg cactcatcct cgtcgcggtc agcccgaaca tcatcaatcc ggtcgaaggt
     1321 gctgcattct tcgtcggcga accgctcttc ccgttcgtca atccggcgat catctccatc
     1381 ccagccgggt tcattgccgc aatcgtcggc acatacgtcg gggcgaaacg caacgaagta
     1441 gatgaaatcg cctatgccga agtccgtttc aaagcagaaa caggcttccg cgggttggaa
     1501 ggccaaacac cgaattgatg gtttgaatga aatacgaaaa gccctcggga acggatttta
     1561 ccgttttccg agggcttttc gttgtgataa atcaataatg ggcgacgcct tcccgcttca
     1621 tttccgtcat ttgatacggg tcgagcaagc gggtaagttc atcaagagtg agtacattgt
     1681 aacgcagaca gagctctttg agcgggacgc ctgtttcgaa cgattcacgt gcgattttcg
     1741 aagacatttc atagccgatg aatggcgtga gtgctgtgat tgtgccgatg cttctttcca
     1801 catattcctg catttgcgaa acattcgcgg tgatgccgct gatgcaatac ttgctgaaca
     1861 cttcgattcc gttgttcatg accgtcaagg attgcaataa attatagacg aggacaggct
     1921 ccatgacatt caattcaaat tggccagctt cggaagcgag gctgatcgta tggtcgttgc
     1981 cgatcacttg gaaagcgatc tggttcatca cttccgccat gacgggattc actttccccg
     2041 gcataatgga ggaacccggc tgccttgccg gaagattgat ttcattcaag ccggctttcg
     2101 gacctgacgc catgagccgt aaatcatttg ccattttcga gagattgatc atgcatattt
     2161 tcaacgctga agagagctcc gtgtatacat cggtgttctg cgtcgaatcg atgaggttgc
     2221 cagctgattt caacggcagg ccgctgatcg tccggagatg ctccaccgcg atttcaatat
     2281 attgcgcatt cgcattcagc ccggtgccga ttgctgttgc gcccatattg atttcggaaa
     2341 gatggaccct tgtccttccg atccgttcaa tatcgcgctc gatgacactg cggtacgcgc
     2401 tgaattcctg gcccaaccga acgggaacag cgtcctgcaa gtgcgttctt cccatcttga
     2461 tgacgccgtc gaattccttt tccttcgcct tcagctcgtc cacaaatttc ttcatcgttt
     2521 ccagcaattg attgaccatt tgctgcgtcg caattttaat cgctgtcggg aatgcgtcat
     2581 ttgtcgactg ggacatattg acgtggttgt tcgggctaat gaagccgtaa tccccttttt
     2641 cacgccccat catttcaagc gcgcggttcg cgatcacttc gttcgcattc atgttgaatg
     2701 aagtgcccgc tcccccttga atcggatcga cgatgaactg gtccgtcaac cttcctttga
     2761 tgacctcgtc tgcagctcca acgatggcga gccccacatc ttcgggcaag tcattgacaa
     2821 gcacattcga aatggctgcc gctttcttca caatcccgat agcacggatc aattccggat
     2881 gcatgcgctc cttcgtgatt ggaaagtttt cagtcgcccg tatcgtctga atcccgtaat
     2941 aggcatgatc gggtacttct ttctccccta agaaatcctt ctcgattctt ccggatagca
     3001 aattcattgt accttttcct ctcgttatgg atatcgccaa taaggcgtac gatgaacaat
     3061 tcctaggcag atttattccg cctggcttca tgtacatcgt acgcctttta tcgtaggaat
     3121 gctatggttt ttttggttat attcgagaga aaaaaccatc cccgtttata gatgctttat
     3181 tcaaacactg ttgtccactc agcccgtttt ccgagcatct ttttcgcagc ttcctttgca
     3241 ccttcaaggc tatggctcgc agcccatccg cattggactt cattgcaagc aggcacttcg
     3301 tcggcggcca aaacgtcgtt caatgtcttt tccaatatgt cgagcacgtc ttcataatcg
     3361 tcgtgattga tgagcgccaa ataataaccc gtctggcatc ccatcggact gatgtctacg
     3421 attctgtcag aatggttcct gctgaactcc gccatcatat gctccaacga atgaatcgca
     3481 ggcatttcaa gatgctcttg attcggttgg caaaaacgga tgtcatactt ccggatgacg
     3541 tcgccattgg ccccttcctt caccccagct agacgcacaa acggcgcttt cactttcgtg
     3601 tggtccaaat tgaagctttc cacattcatc ttcttttctg tcgtcgacat gtccaccact
     3661 ccttttcatc gcatccgcgc aatcaaatat atcctattga tttacttggg tttatttcag
     3721 aatatcaaaa ttctgaatag atagcaagat acaatgataa actcctacac cgattgccaa
     3781 tcattttcaa taaacgcttt tttgtggcac gatggaggaa tgaaggagtt gattcgatga
     3841 tacgttttga cggtgtaaca aaatcgtatg acaatcaaac gcttgtcgtt gattcgttca
     3901 atttagaggt tgccaaaggg gaatttctcg tgctgatcgg gccgagcgga tgcggcaaga
     3961 caacgacgct gaaaatgatc aatcgcctca ttccgctgac ggacgggacg atttggatcg
     4021 acggcaggaa ggtcagcgag tacgcaatcc atgaattgcg atggcggatc ggctacgtgt
     4081 tgcagcagat cgccttgttc ccccatatga cgatcgagga aaacatcgca atcgtccccg
     4141 agctgaagaa ttggaataaa agccggatcc atcaccgtgt gacggaactt cttgaaatgg
     4201 tgggactcga tccggataca taccggcacc ggaagcctca tgaactatcc ggcggccagc
     4261 agcagcgggt cggcgtcgtc cgtgcccttg cagcagatcc cgatatcata ttaatggatg
     4321 aaccgttcag tgcacttgat ccgatcagca ggggtaagtt gcaggaggac ctgctgcagc
     4381 tgcaacgttc cattcagaaa acgattgtat tcgtcacaca cgatattcag gaagctatga
     4441 aacttggaga ccgcatttgc atcatgaagg acggcaaaat cgaacagctt ggcaccccgg
     4501 atgaaattat gttgacccct gccacgccgt tcgtccgcaa attcatccag agcggcagcc
     4561 agaagccatt cgtactatcc gagcatatcg aaccgatgac gacagatacg gaacgattgc
     4621 ccgtattatc cgtcaacgcg gggtgggatg aagtgctgca tgcgctatcc ctatcggacc
     4681 gggtccttat ccagcaagaa ggagcaccga tcggcaccat ttcaagacag acgatcatcg
     4741 cctatttggc aagcggtcaa gaaggaggaa agatagcgca tgggtgagtt tataggcgtt
     4801 tttcaagata ggaaaggcga gttggcttgg gcattgatgg agcatatcca aatttcgctc
     4861 attgcactct tcttcgctgt tctcatctcc atcccggtcg gcgtttattt gacaagccgg
     4921 cgacggatcg cggaaacagt catcgggaca agcgcagtgc tacagaccat tccgtcactt
     4981 gctctgctcg gattgctgat cccgctgttc ggaatcggaa aggtccctgc catcatcgca
     5041 ctcgttgcct atgcattgct gccgattttg cgcaacacct atacaggcat caagggaatc
     5101 gatccttcct tgatcgaagc ggcgactgcg ttgggcatga atcgacggaa gcgtctgacg
     5161 aaagtcgaac tgcctctcgc catgcctgtc atcatggcgg gcatccggac atcgatggtg
     5221 ctgattgtag gaaccgccac acttgctgca ctgatcggtg caggcggtct cggcgatctc
     5281 atcctgcttg gcatcgatcg caacagccct tcccttatcg tgctcggtgc aatcccagcc
     5341 gcccttctcg ccctgtcatt cgacttgctg ctaagaagat tcgaggggct ctcgttccga
     5401 caggcttcca tggcactgac cgcattttcg gtcgcggccg tactgatcat ctccctgccc
     5461 ctcttccctg caagcaatga aagacagctt gtcatcgggg caaaattagg ggcggaacct
     5521 gaaatactca ttaacatgta caagctcctc atcgaagaag agacggacct gtccgtcgaa
     5581 ctgaaaccgg gacttggcaa aacatcgttc gtcttcaacg ccttgcaatc agggagcatc
     5641 gatatctatc ccgaattcac agggacggcg ttgtcggagt tcttgaaaga ggcagccctc
     5701 agcaatgacc ggaacgaagt atacgagcag gcgcgtgtag ggcttgccga gcaattcgac
     5761 cttgtcctcc tgccgccgat ggagtataac aatacatacg cgattgcggt atccaaagcg
     5821 tttgaggaac agtacggtgt caagacaatt tccgatctct tccctgtcca aactgccgta
     5881 aaagctggat tcaccctcga attcaatgac cgggaggacg gctaccgggg catccggagc
     5941 ttgtacggaa tcgacttccc ttccgtccaa acgatggagc cgaaactccg ctaccaggcc
     6001 atccgatccg gggatatcaa cctgctggat gcctattcga cggacagtga actgcagcaa
     6061 tatgaactta ccgtcctaca agatgacaaa gagctgttcc cgccctacca aggggcccct
     6121 ctcctgcgga aagaaaccct cgaaacgtat cccgaacttg aggaaatcct caaccggctc
     6181 gccggcaaag tgacggacga cgagatgcgg gacatgaact acagggtgaa cgcagaaggt
     6241 gaaaaaccgg aagacgtcgc acgccagttt ttacaggaag caggtttatt ggaataaaac
     6301 aaaaggaacg attgccggtt gtgcattcgt tccttttatt caccctatat gcttcaaagc
     6361 ctctcgcctg cttaacggtt tcagcgaatg atcttcgaca aactgcttca caataactgg
     6421 atccgttttc gcatactccc tcaataccca accgatcgct ttttgaatga aaaactcatt
     6481 tgattccaca tgctgcaaaa tgatttcgaa taacagctcc gtatccgttt cttttttaaa
     6541 ctttaattgg tgcagaatgg cggagcggtt tgtccacata ttctccgatt gcgaccattc
     6601 aagcattact gttttcctgt aatcacgatc caatttgacg acgtgcccga caagatgcgg
     6661 cgcaatcgaa tcgacactat cccaccacga tttcgtttca atgaatttca gcagcttcgg
     6721 ataatcatca gtcgtgaaat gcttcttcat cttttcaagc aacgcgattg cggcatactg
     6781 gtattcccgc tcgggcaatt catatagctt ccatgcctcg gcaaaagcct cgccagcttc
     6841 aggcaacgca tattcacgga agtgctcgcg cagcagctcc ttccgcagcg gacttttaat
     6901 gccaagaaac gggaaatggt gcttcatata cgctttcatg gggcctgctt gctcgacatt
     6961 ccggcacatt tcaaacttct cgataattcc tttatggttc cattgctttt tcactcgagt
     7021 caactccaca ttttacatac tatttagaat tacaatatca ttaatcgaac acacgttcaa
     7081 ctcttcgtga tatttaaaag cgcctcatac aacaaatcaa aataaaaaac cgccccatga
     7141 caaaccctgc gacggtaacg taattgcacg atataatcag cttggcagca aacgacgggc
     7201 caacacaaga atcccctaac tggttcccca agatttgaaa gagccttgag cttttagcgt
     7261 taatctttaa attgtaaaga acttt
//
LOCUS       DASULI010000381         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1587516,
            whole genome shotgun sequence.
ACCESSION   DASULI010000381 DASULI010000000
VERSION     DASULI010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587516"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFX34_08220"
     CDS             <1..716
                     /locus_tag="VFX34_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX5564931.1"
                     /translation="EYGLYPDFDASSDFDVDETRDRVNQLKSEIESLGSVNPSAIEEY
                     KEVSERHTFLTEQRNDLLEAKNTLNEAMSEMDREMEMRFGTTFNAVRNRFHVVFKEMF
                     GGGDADLILTEPDNLLETGVEIVARPPGKKLQNLSLLSGGERALTAITLLFSIIEVRP
                     VPFCILDEVEAALDEANVIRYSKYLKKFSDNTQFIVITHRKGTMEGADVLYGITMQES
                     GVSKLISVKLSDVPAEVLG"
     gene            750..1739
                     /gene="ftsY"
                     /locus_tag="VFX34_08225"
     CDS             750..1739
                     /gene="ftsY"
                     /locus_tag="VFX34_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008430830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX5564932.1"
                     /translation="MSFFKKLKEKITGTNETVTEKFKDGLSKTRNSFTSKVNDLVARF
                     REVDEEFFEELEELLLQADVGVETVMELVDQLRWEVQRKNIKSTEGIQSVISEKLVEI
                     YKDGEELDTDLNMQDDLTVILMVGVNGVGKTTTIGKLAARLKEEGKTVMLAAGDTFRA
                     GAIDQLVVWGDRVGVEVIRQSEGSDPAAVMYDAIRAAKSRKVDVLICDTAGRLQNKVN
                     LMNELEKVHRVIGKEIEGAPHEVLLALDATTGQNALIQAQTFKEATNVTGIVLTKLDG
                     TAKGGIVLAIRNKLNIPVKFVGLGEGMDDLQPFDPEKYVYGLFADGLEKEAEE"
     gene            1836..2168
                     /locus_tag="VFX34_08230"
     CDS             1836..2168
                     /locus_tag="VFX34_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEX5564933.1"
                     /translation="MLEKTTRVNFLYDFYQTLLTEKQRVYMQLYYLDDLSLGEIAEEY
                     GVSRQAVYDNVRRTEAMLEDYEDKLKLFSKFRQRMEIIEKLERLILGNDKVSEEVLML
                     INALKDHE"
     gene            2181..3536
                     /gene="ffh"
                     /locus_tag="VFX34_08235"
     CDS             2181..3536
                     /gene="ffh"
                     /locus_tag="VFX34_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5564934.1"
                     /translation="MAFEGLAQRLQGTLQKIKGKGKINEADVKEMMREVRFALIEADV
                     NLKVVKEFVKTVSERAVGQDVMKSLTPGQQVVKIVKDELTELMGGEQNPIQFARKAPT
                     VIMMVGLQGAGKTTTTGKLATVLRKRHNKKPLLVAADVYRPAAIQQLETLGKQLTMPV
                     FSLGTDVSPVEIARQALAEAEKEHHDVVIIDTAGRLHVDENLMQELKDIRELSKPDEV
                     FLVVDAMTGQDAVNVAKSFDEAIGITGVVLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     GEKMDALEPFHPERMASRILGMGDVMSLIEKAQESVDEEKAKELEQKFRTQTFTLDDF
                     LDQLQQVKKMGPLDEILKMMPGMNKIKGLENAQVDESQMGRVEAVIRSMTTAERTTPE
                     IINASRRKRIAKGSGTSIQEVNRLLKQFEDMKKMVKQMTNMQQKGKKKMKMPGFDSLF
                     R"
     gene            3649..3921
                     /gene="rpsP"
                     /locus_tag="VFX34_08240"
     CDS             3649..3921
                     /gene="rpsP"
                     /locus_tag="VFX34_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5564935.1"
                     /translation="MSVKIRLKRMGAKKTPFYRIVVADSRSPRDGRQIETVGTYNPLT
                     KPAEVKIDEELALKWLQNGAKPSDTVRNLFSEQGIMEKFHNAKFGK"
     gene            3945..4178
                     /locus_tag="VFX34_08245"
     CDS             3945..4178
                     /locus_tag="VFX34_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX5564936.1"
                     /translation="MKQLIETIVKPLVDHPGDVQVDKDEQHHRVTYKLSVNPEDMGKV
                     IGKQGRVAKAIRTIVYSAAGSRHGKKVYLDIVD"
     gene            4328..4843
                     /gene="rimM"
                     /locus_tag="VFX34_08250"
     CDS             4328..4843
                     /gene="rimM"
                     /locus_tag="VFX34_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828270.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5564937.1"
                     /translation="MQWFNVGKIVNTHGIHGEVRVISRTDFPDERYAVGNELSLFMPN
                     EKKPIKLIVASHRRHKNFDLLSFEGHININDVEKYRDGILKVSEKQLGDLDEGEFYYH
                     EIIGCTVYTDVGEELGEVTEIIETGANDVWTVTPKTGKPHYIPYIEDVVKEVDIEKKQ
                     IIIEPMEGLLS"
     gene            4840..>5543
                     /gene="trmD"
                     /locus_tag="VFX34_08255"
     CDS             4840..>5543
                     /gene="trmD"
                     /locus_tag="VFX34_08255"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5564938.1"
                     /translation="MMKIDVLSLFPEMFEGVLHSSIMKKAQENEAVSFKVTDFRDFSG
                     NKHHKVDDYPYGGGAGMVLKPEPLFAAVESLALDQSKKPRVILMCPQGARFNQKKAEE
                     LSREEHLVFLCGHYEGYDERIREHLVTDEISLGDFVLTGGEIASMAIIDSVVRLLPNV
                     LGNTDSPVHDSFSTGLLEHPQYTRPSDFRGMKVPDVLLSGNHAEIEKWRQEQSLKRTF
                     KRRRDLLDNAPLTEHQ"
BASE COUNT         1721 a         1159 c         1396 g         1267 t
ORIGIN      
        1 atgaatacgg cctctacccc gattttgatg caagcagcga ttttgatgtg gatgaaacaa
       61 gagaccgggt caaccagttg aaaagcgaaa tcgaatcgct cggttcagtt aacccgagtg
      121 caatcgagga gtacaaagaa gtgtctgagc gtcatacatt tctgactgaa caacggaacg
      181 atctcttgga ggcgaaaaac acgctcaatg aagcgatgtc cgaaatggac cgcgaaatgg
      241 aaatgcggtt cggtacgaca ttcaatgcgg tcagaaatcg gttccatgtc gtcttcaaag
      301 aaatgttcgg cggaggagac gcagacttga ttcttacaga gcccgacaat ttattggaaa
      361 cgggagttga aatcgtcgca agaccacctg gcaaaaaact gcagaatctt agcttgctat
      421 ccggcggtga aagggccctc acagcgatta cattgctgtt ttcgatcatt gaagtacggc
      481 cggtcccatt ctgcattttg gacgaagttg aagcggcgct cgacgaagcg aatgtcatcc
      541 ggtacagtaa gtacttgaag aagttctcgg acaataccca attcatcgtc atcacccaca
      601 ggaaagggac gatggaaggg gcggatgtcc tttatggaat cacgatgcag gaatccggag
      661 tatcgaaact gatttccgtg aagctttccg atgttcccgc cgaagtgctg ggataaggat
      721 ggacagtata gaaaaaggag agagtcggat tgtctttttt taagaaattg aaagagaaga
      781 ttacgggcac gaatgaaacg gttacagaaa aattcaagga cggtctgtcg aagacccgaa
      841 attcctttac ttcaaaagtg aacgatcttg ttgcacgttt ccgtgaggtc gatgaggagt
      901 tcttcgaaga gttggaggag ctattgcttc aagcggatgt cggcgtcgaa acggtcatgg
      961 aacttgtcga tcaactgcgt tgggaagttc aaaggaaaaa tatcaagagc accgaaggca
     1021 ttcaatcggt catttcagag aagctcgtgg aaatctataa agatggcgaa gaactcgata
     1081 ccgatttaaa tatgcaggat gatctaactg tcattttgat ggtcggagtc aacggtgtag
     1141 gaaaaacgac tacgatcgga aagcttgcgg cccgccttaa agaggaaggg aaaaccgtta
     1201 tgctcgcggc gggggatacg ttccgtgcag gcgcaatcga tcagctagtc gtttggggcg
     1261 atcgagtcgg agtcgaagtg atcaggcaat cggaaggatc agatccggcc gctgttatgt
     1321 acgacgctat tcgcgccgca aaaagccgta aggtggatgt cttgatctgc gatacggctg
     1381 gcagattgca aaacaaggtg aacttgatga atgagctcga gaaagttcat cgtgtcatcg
     1441 gaaaagaaat cgaaggcgct ccgcatgagg tgcttcttgc acttgacgcg acgacaggtc
     1501 aaaatgcatt gatccaagcc cagacgttca aggaagcaac gaatgtcacc ggaatcgtac
     1561 taacgaagct cgatggtacg gcaaaaggcg gcatcgtatt ggcgatccga aataaactca
     1621 acatcccagt caaatttgtc ggcttaggtg aaggcatgga cgacctccaa ccattcgatc
     1681 ccgaaaaata tgtatacggc ttatttgcgg atggtctgga gaaggaagca gaagaataat
     1741 catttgcaat ccagcttcaa catctttgca gacaagtaaa tttacttgac agttccattc
     1801 acgcatacta agattgacag aggaggcgaa gcgcaatgct tgaaaaaacg acacgcgtca
     1861 acttcctcta tgatttctat cagacactgt tgaccgaaaa acaacgggtg tatatgcagc
     1921 tctattacct cgatgacctt tcactcggcg aaattgccga agaatatggt gtgtcgagac
     1981 aggctgtcta tgacaatgtc cgaagaacgg aagctatgct agaagactac gaagataaac
     2041 tgaagctttt ctccaaattc cggcaacgga tggagataat cgaaaagctt gaacggctta
     2101 tactcggaaa tgacaaagtc tccgaagaag tcctgatgct gataaacgca ttgaaagatc
     2161 atgaataggg ggttctatgc atggcatttg aaggtttggc ccagcgcctg caagggacgc
     2221 ttcagaaaat aaagggcaaa ggcaagatca atgaggcaga cgtcaaagaa atgatgcgag
     2281 aagtgcgatt tgcgcttatc gaagcagacg tcaacttgaa agtcgtcaaa gaattcgtca
     2341 agaccgtcag cgagagggct gtcggccaag atgtcatgaa aagcctgaca cctggccagc
     2401 aagtcgttaa aatcgtaaaa gatgaactga cggaattgat gggcggagag caaaacccaa
     2461 tccaatttgc aagaaaagca ccgacagtca tcatgatggt cggcttgcaa ggggcaggta
     2521 aaacaacgac aacggggaaa ctcgcaacag ttcttcgtaa gcgtcataat aaaaagcccc
     2581 tccttgtagc ggcagacgtt tatcgcccag cagcaatcca acaattggag acacttggaa
     2641 aacaattgac gatgcccgtc ttttcattgg gcacggatgt ctccccggtt gaaattgcgc
     2701 ggcaagcgct tgcggaagct gaaaaggagc atcatgatgt cgtcataatc gatacggcgg
     2761 gacggttgca tgtcgatgaa aacctgatgc aggaattgaa agatatacgg gaattgagca
     2821 agcccgacga agtgttcctc gtcgtcgatg cgatgacagg gcaggatgcc gtcaatgtgg
     2881 cgaaatcatt tgacgaagca atcggtatta ccggagtcgt cctgacaaaa ctggacggcg
     2941 atactcgtgg cggggctgca ttgtcgattc gatccgttac gcagaagccg atcaagtttg
     3001 tcggtatggg tgagaagatg gacgccttgg aacctttcca tccagagcgg atggcctcaa
     3061 ggatcctagg aatgggcgac gtcatgtcac tcattgaaaa ggcacaggaa agtgtcgatg
     3121 aagaaaaagc gaaggagctt gaacagaagt tccgcaccca gacattcaca ctcgacgact
     3181 ttttggatca gctccagcaa gtgaaaaaga tgggtccttt ggatgaaatt ttaaaaatga
     3241 tgcctgggat gaataaaatc aaagggcttg aaaacgccca agtcgatgaa agtcagatgg
     3301 gacgagttga agctgtcatc cgttcgatga caacagcaga acgcacgaca cctgaaatca
     3361 tcaatgcgag caggcgcaag cggattgcga aaggatcggg tacttcaatc caggaagtga
     3421 accgtctctt gaagcaattc gaagatatga agaagatggt caagcagatg accaatatgc
     3481 agcaaaaggg caaaaagaaa atgaaaatgc ctggtttcga ctctttattc aggtgaaatc
     3541 gtagaattct aaagtaattt taaggtgtta agaaaaaaca ctttacaaac atgaaaaacc
     3601 ttgataatat actatcttgt gtgaaactat tcggaggtgc aagaaaaaat gtcagtaaaa
     3661 attcgtctta aacgtatggg agcaaagaaa actcctttct atcgtattgt tgtagcagat
     3721 tcacgttcac cacgtgatgg ccgtcaaatc gaaacagtag gtacttacaa cccacttaca
     3781 aaacctgctg aagtgaaaat cgatgaagaa ttggctctta aatggcttca aaacggtgct
     3841 aaaccttctg atacagttcg taacctgttc tcagaacaag gcatcatgga gaaattccat
     3901 aacgctaaat ttggtaagta atagtcatat caggaggggt ggacatgaag cagctgattg
     3961 aaacgattgt caaacctttg gtcgatcatc ccggagatgt tcaggtcgac aaagatgagc
     4021 aacatcatcg tgtcacttat aaactttccg taaaccccga agatatgggg aaagtgatcg
     4081 gaaagcaggg gcgtgtcgca aaagcgatac gaactattgt ttattcagca gcaggaagtc
     4141 gccacggtaa gaaagtttac cttgatattg tcgattgact ggtatcaatc atgaacaaca
     4201 agtttctgaa tgctggccca tgcttgccta ggaaacctgc acaaaaaagg agggagctca
     4261 tgttccttcc ttttttgtct acttatggaa tgcatccatg tccctaatga aaaggaaggt
     4321 gtattgtatg caatggttca acgttggaaa aattgtcaac acacatggaa tccacggcga
     4381 agtgcgtgtc atttcgcgta ctgatttccc ggatgaaagg tatgctgtcg gaaacgaact
     4441 ttcattattc atgccgaatg aaaaaaaacc gattaagcta atcgtagcaa gccaccgccg
     4501 ccacaaaaac ttcgaccttc tttcctttga aggacatatc aatatcaatg atgttgaaaa
     4561 atatcgggat ggaatcttga aagtttccga aaagcagctc ggtgatcttg atgaaggaga
     4621 gttttactac catgaaatca tcggttgcac tgtctataca gatgttggag aagaattagg
     4681 cgaagtgact gagataattg aaactggagc caacgacgtg tggacggtaa cgccgaaaac
     4741 tgggaaacca cattacatcc cttatattga ggatgttgtt aaagaagtcg atattgaaaa
     4801 gaaacaaatt atcatcgaac cgatggaagg gcttctttca tgatgaaaat agatgtcctc
     4861 tcgctgtttc ctgaaatgtt cgaaggggtt ctccactctt ccatcatgaa aaaggcacaa
     4921 gaaaacgaag cggtttcatt caaagtgacc gatttcaggg acttctccgg caacaagcac
     4981 cataaagtgg acgactaccc ttatgggggt ggcgcgggaa tggtattgaa gccagaacct
     5041 ctatttgcag cagtcgaatc gctcgccctc gatcaatcaa agaagccgcg cgtcatcctc
     5101 atgtgtcccc agggcgctag gttcaatcag aaaaaggctg aagaactctc aagggaagaa
     5161 catctggtat tcctctgcgg ccattatgaa ggctatgacg aacgtatccg tgagcatctt
     5221 gtcactgatg aaatatcgct cggagacttc gttctgactg gcggggaaat tgcctccatg
     5281 gcgatcatcg acagcgtcgt cagactcttg ccgaatgtat taggtaatac ggactcaccc
     5341 gtccatgatt cattctcaac aggacttctc gaacatcccc agtacacaag accatccgat
     5401 ttcaggggca tgaaagttcc cgacgtcctc ctatcaggca accacgcaga aattgaaaaa
     5461 tggcgccagg aacaatcact aaaacgtaca ttcaaacgca gacgggatct attggataac
     5521 gctccattga ctgaacatca gca
//
LOCUS       DASULI010000382         9199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_868032, whole
            genome shotgun sequence.
ACCESSION   DASULI010000382 DASULI010000000
VERSION     DASULI010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 9199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9199
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868032"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFX34_08260"
     CDS             complement(<1..724)
                     /locus_tag="VFX34_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX5564939.1"
                     /translation="MKLARNIIIALIAGVLVGLILNLFAPGVFGFTDKYLFKPLGTIF
                     LNLMKMLVIPVVFVSIALGTIGIGDPKKLGRIGGKTLGYFLATTAIAIVIALSLALML
                     KPGAGGNFDTSGANYEAEEAPPVTDTLLGIIPTNPVTAMAEGNMLQIIFFAALVGFGI
                     AMLGKRVERVTELLEQVNELIMYLITFVMKFAPYGAFGLIASAVGSQGFDALQAMGMY
                     MGVVLGALLIHSIVVYGGTVSLL"
     gene            complement(895..1068)
                     /locus_tag="VFX34_08265"
     CDS             complement(895..1068)
                     /locus_tag="VFX34_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564940.1"
                     /translation="MERVFIDPQEMELRQDLDFIASLEMIDEGSPVHYPQYAKNMKEA
                     AGFYPNELPQAYQ"
     gene            1271..1663
                     /locus_tag="VFX34_08270"
     CDS             1271..1663
                     /locus_tag="VFX34_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX5564941.1"
                     /translation="MKRIVLFDGECNFCNSSVQFIIKRDPAAHFLFSSLQSEIGEKYV
                     KQYNIPDDVDSIVLIENGKAYTKSSAALHIAKKLDGLWHLLFLFILVPRKVRDGFYDY
                     FARNRYRWFGKNEDACMLPSPEVRKRFI"
     gene            complement(1771..2133)
                     /locus_tag="VFX34_08275"
     CDS             complement(1771..2133)
                     /locus_tag="VFX34_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEX5564942.1"
                     /translation="MPERVLATDAALELIELLKGKHGPLMFHQSGGCCDGSSPMCYPE
                     GDLIIGDQDVLLGHIGGTPFYMHKNQFEYWKHTQLIIDVVEGRGGMFSLEGVEGKRFL
                     TRSRAFTSEENEKLRQEA"
     gene            complement(2175..3716)
                     /gene="adh"
                     /locus_tag="VFX34_08280"
     CDS             complement(2175..3716)
                     /gene="adh"
                     /locus_tag="VFX34_08280"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX5564943.1"
                     /translation="MVQAVENHVYAFPNTEGAKVNFKERYDNFIGGKWVAPVNGQYFD
                     NPTPVTGKVFTQVARSTAEDTELALDAAHAAKDAWGKTSVTERANILLKIADRMEANL
                     EMLAVAETWENGKAVRETLNADLPLAIDHFRYFASAIRSQEGGVSQIDNNTVAYHFHE
                     PIGVVGQIIPWNFPLLMAVWKLAPALAAGNCVVLKPAEQTPASILVLMELIEDLLPAG
                     VVNIVNGFGLEAGKPLASNPRIGKIAFTGETTTGRLIMQYASQNLIPMTLELGGKSPN
                     IFFEDVMAEDDEFLDKAIEGFVMFALNQGEVCTCPSRALIQESIYDKFIERAIERVKA
                     IKTGNPLDPTVMMGAQASTEQLEKILSYLDIGKQEGAECLTGGEQNKLGGELADGYYV
                     KPTVFKGTNKMRIFQEEIFGPVVSVTTFKTKEEALEIANDTLYGLGSGVWTRDMNTAY
                     RFGRGIEAGRVWTNCYHAYPAHAAFGGYKASGIGRENHLQMLSHYQQTKNMLVSYSED
                     KLGFF"
     gene            4073..5233
                     /locus_tag="VFX34_08285"
     CDS             4073..5233
                     /locus_tag="VFX34_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009335124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5564944.1"
                     /translation="MANSFGKRFVSTVGAGILGSALTLGVVANTDILQAKTQKAQTAA
                     VTESSSSSPNFVQTSANAPTSLSDMVEHASKGIVGVSNFKNTGNRFAGSSELSEFGTG
                     SGVIYKIDGDDAYIVTNNHVIEGAGKIEITLHGGEKETAELIGSDALTDLAVLKINSK
                     QVDTALEFGDSDKLRAGDSVVAIGNPLSLDFSGTVTQGIISAPARSIDVKTTAGNWEM
                     NVIQTDAAINPGNSGGALLSTEGKVIGINSLKIAENGIEGLGFAIPSNDVVPLLEQIT
                     ENGKIERPYIGVGLADLADVPYMYVQNLPQEVKGGVMIASVDPTSAAGKAGLKEQDVI
                     TAINGTDILNSMELRKFLYSELKIGDKATLTVYSGSQKRTVELTLTSNSPVE"
     gene            complement(5283..6236)
                     /locus_tag="VFX34_08290"
     CDS             complement(5283..6236)
                     /locus_tag="VFX34_08290"
                     /inference="COORDINATES: protein motif:HMM:NF020050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HEX5564945.1"
                     /translation="MKTKIHIISIQEGYLTIISEQLELIFGDKATISKLVVKDLDGDS
                     IAEEDIVVLSRSILMGVIRPFIPKDCKTIIAHREVNIANTKRLIDLPAGQKLLVVNDT
                     LENAQETLDSLMNIFFTHTYYVGDENNPDQTIPEDIDYIVTPGEMEFVPSRFSNVIDI
                     GDRLLSYGTVWEIAEALEAGFTHEQLVNRYVKSQVSLAEPGITGIASERETLADAANL
                     SHNEILSIDKKIEEHGFLKESIQILSIYADGKAQLKTFGRIKLKKLLEEHDIILSEQQ
                     IRLRLEVLQELGLLNARQGRGGTTITSLGESYLQAKDLVKS"
     gene            complement(6325..8331)
                     /locus_tag="VFX34_08295"
     CDS             complement(6325..8331)
                     /locus_tag="VFX34_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564946.1"
                     /translation="MKPLLEVKDLQVSFHTQAGVVKAVRGVDFTINEGETLAIVGESG
                     SGKSVTSKAIMKLLPDRIASYDGGQVLFDGQNLLEAPERRMEVVRGSEIAMVFQDPMT
                     ALNPTMTIGKQVAESVMKHQGLSKKEAKQRAIEILELVGIPEPATRVNQYPHQFSGGM
                     RQRVVIAIAIACNPRLLIADEPTTALDVTIQAQILDLLKDIQKRMGTAIILITHDLGV
                     VANMADKVAVMYAGKIVEEGTVNEVFYESQHPYTWGLLKSIPDLDAEEELYSIPGSPP
                     DLLHPPVGDAFAVRNEFALAIDYKTQPPMFKVSDSHSAATWLLHPKAPDLSTVIQVKE
                     RERVAVAKKRKSRIADLSPIIQVKDLRKHFHLSGNKHLRAVDGVSFDIHKGETLGLVG
                     ESGCGKSTTGRTIIRLYEPTGGEVYFDGEPIHGQLKKGQENRMKQKIQMIFQDPYASL
                     DPRKKVLDSVAEGIDIHGLAASPKDRKDIVQNLLEIVGLNKEFMNRYPHEFSGGQRQR
                     VGIARALAVNPDVIIADEPISALDVSIQAQVVNLLKDLQYENDLTYLFIAHDISMVKY
                     ISDRIGVMYLGNLVELAPTKALSEKPLHPYTQALMSAVPVPDPVEERGRERIVLTGDL
                     PNPMNPPTGCRFRTRCPYAMEKCKETPIWKEAERDHWVACHLID"
     gene            complement(8357..>9199)
                     /locus_tag="VFX34_08300"
     CDS             complement(8357..>9199)
                     /locus_tag="VFX34_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5564947.1"
                     /translation="QMSMFKESWIRLKKNKGAMLSLVILSILIVMAIIGPHLNEYSYE
                     QTDYDKVFQKPSGEHWLGTDRFGRDQWTRIWEGTRISLYIAFLAATLDLIIGVMYGAV
                     SALLGGRVDNVMQRIIEVLVGIPNLILIILLIMLMKPGIVTITIAMVITGWVNMARLV
                     RSQVFKLKGQEFILASRALGATNTRLIKEHFIPNTLGLIIVNMMFTIPAAIFTEAFLS
                     FIGLGLQEPLASLGVLINDGFQAMRNHFYLLLFPALVIVGLMVCFNLLADGLRDALDP
                     KMKK"
BASE COUNT         2253 a         2411 c         2091 g         2444 t
ORIGIN      
        1 cgagcaagga caccgtgccc ccgtagacga cgatcgagtg gatgagaaga gcgcctagga
       61 caacacccat atacatcccc atcgcctgca gcgcgtcgaa tccttgactg ccgactgctg
      121 aagcaatcaa gccgaaagct ccgtatggtg caaacttcat gacgaaggtg atgagataca
      181 taattagttc attcacctgt tccagcaatt cggtcactcg ctccacccgc ttgccgagca
      241 tcgcaattcc gaatccgacc aaggctgcaa agaagatgat ctgcagcata ttcccttccg
      301 ccattgctgt gaccgggttc gtcgggatga tacctaataa cgtatccgtg acaggcggtg
      361 cttcttccgc ttcataattc gctcccgatg tatcaaagtt ccctcccgca cccggcttca
      421 acatgagggc caaggagagg gcaatgacga tggcgatagc ggttgtggct aggaaatacc
      481 ccaacgtctt cccgccaatc cttcctaact tcttcgggtc gcctatgcca atcgttccaa
      541 gcgcgatgga aacgaagacg accggtatga cgagcatctt cattaggttc aggaagatgg
      601 tacctaacgg cttgaacagg tacttgtccg taaagccaaa aactccgggg gcaaacaagt
      661 tcagaatgag tccgacaagg acacccgcaa tgagggctat gataatattg cgtgctaact
      721 tcatggcgat tcctccttca tccatttttc cgaagcacta aagcgaaacg ctctctttta
      781 tgctatctgc ctatctatcc cttgtttttt aaattttgaa acttcaatcc ttctagaata
      841 gggcaataaa aaagagctgt cccctttgat tcgagacagc tccgttacac ttacttattg
      901 atatgcttgt ggtaattcat ttggataaaa tccggcagct tctttcatat tctttgcgta
      961 ctgcggataa tggacaggtg aaccttcgtc gatcatctca agtgatgcga tgaaatccag
     1021 atcctgcctc aattccattt cttgtggatc aatgaatacg cgttccattc aatcactctc
     1081 cttcaaatgt tacccaaggc aaatgtgaca ggaagtgaag acaatgtcca cgataggaaa
     1141 aaccgcggga ttatcttctg ctactattag tataccaaat tttctgtatt aagtaaagaa
     1201 atgtagattg tgtttcattt tgtccgataa ggatataatg aaactaagca tgtcggaagg
     1261 agatgccata atgaagcgaa ttgtgttatt cgatggtgaa tgcaattttt gcaactcaag
     1321 tgtgcagttc attataaaac gggatcctgc agcgcacttc ctattttctt cattgcaaag
     1381 cgagatcggc gaaaagtatg tgaagcaata taacattccg gatgatgttg acagcatcgt
     1441 gttgattgaa aacggaaagg cgtacacaaa atcttcagcg gctctccata tcgcaaaaaa
     1501 gttggatggc ctgtggcacc tcttgtttct atttatactc gtcccacgta aagtccgtga
     1561 cggcttttat gactattttg cacgcaaccg ctatcgctgg ttcggcaaaa acgaagatgc
     1621 gtgcatgctt ccttctccgg aggtaaggaa acgatttata taagattatt cgggcgcttc
     1681 ctcaattatg agaggcgcct attttttatt ttaatttttc aaaagtgaac caatgaatat
     1741 aaaaaaggtt ccgggtgccc ggaacctttt ttatgcttct tgccgaagtt tttcgttctc
     1801 ctccgacgtg aaggcgcggg aacgtgtaag gaatcgcttc ccttccaccc cttcaaggga
     1861 gaacatgcct cctcttcctt ccacgacgtc aatgatcagc tgtgtatgct tccaatactc
     1921 aaattggttt ttatgcatgt agaaaggtgt tccgccgata tgtccaagaa ggacgtcctg
     1981 atctccgatg atcaggtcgc cttccgggta gcacatcggg gaggagccgt cgcagcagcc
     2041 tcccgattgg tggaacatca atgggccatg cttccccttc aataattcaa tcaactccaa
     2101 agcggcgtcc gttgcaagaa cgcgttcggg cattggcatt cctcctttca ttcaaaacat
     2161 caaaaatccc tttattaaaa gaagcctaat ttgtcttcgc tatagctgac gagcatattt
     2221 ttcgtctgtt ggtaatgcga cagcatttgc agatggtttt cacgtccgat tccggatgcc
     2281 ttgtagcctc cgaaagcggc gtgtgcagga tatgcatggt agcagttcgt ccagacacgt
     2341 cctgcttcaa tgccgcgtcc gaaacgatat gctgtgttca tatcgcgtgt ccagacgcct
     2401 gaacctaagc cgtataatgt gtcgttcgca atttccaacg cttcttcctt tgtcttgaat
     2461 gtcgtgacgg aaacgaccgg tccgaagatc tcctcttgga agatgcgcat tttgttagtc
     2521 cctttgaaga cagtcggttt gacgtaatag ccatccgcca gctctcctcc gagtttgttc
     2581 tgctctccgc ccgtgaggca ctccgcccct tcctgtttcc cgatatcaag gtacgaaagg
     2641 attttctcca attgctccgt cgaagcttgc gcgcccatca tgactgtcgg atcaagcgga
     2701 ttgccggtct tgatcgcctt gacgcgctcg attgcacgct cgatgaactt gtcgtaaatg
     2761 gactcctgga tcaaagcgcg cgaagggcat gtgcacactt ccccttggtt caaggcaaac
     2821 atgacgaagc cttcgatcgc cttatccaag aattcatcgt cttccgccat gacgtcttcg
     2881 aagaagatat tcggcgattt tccgccaagc tctagcgtca tcggaatcaa gttctgtgat
     2941 gcatattgca tgatgagtcg gcctgttgtt gtctcgcccg tgaatgcgat tttgccgata
     3001 cgcggattcg acgcgagcgg cttgcccgct tcgagaccga agccattgac gatattgacg
     3061 actcctgcag gcaatagatc ctcgatcaat tccataagaa ccaaaatgga tgcaggcgtt
     3121 tgctcagccg gcttcagcac gacacagttc cctgcagcaa gcgctggtgc aagcttccaa
     3181 accgccatca agagcgggaa gttccaaggg atgatctggc cgacgacccc gatcggctca
     3241 tggaaatggt acgccactgt attgttgtcg atttggctga cgccgccttc ttgcgagcgg
     3301 atcgccgaag cgaaatatct gaaatggtcg attgcaagcg gcagatctgc gttcaatgtt
     3361 tcgcggaccg cttttccgtt ttcccatgtt tccgcaacgg ccagcatctc aagattcgct
     3421 tccatgcggt ctgcgatttt caataggata ttcgcgcgtt ccgttaccga cgttttcccc
     3481 caggcatctt tcgcagcatg tgcagcatcc agcgcgagct cagtgtcttc tgcagtcgaa
     3541 cgcgccactt gcgtaaatac tttgcccgtg acaggcgtcg ggttgtcgaa gtattgccca
     3601 ttcaccgggg caacccattt tccgccgatg aaattatcat agcgttcctt gaaattcacc
     3661 tttgcccctt ctgtgttcgg aaatgcataa acgtgattct caacagcttg taccatgttc
     3721 cattcctccc tattctttat agattgctat agtaagtgca aaaattgcac acaaagccac
     3781 catatgcacg agaatgcagc gccattctat ttggatgcgc ttacatttaa tgaatgaaga
     3841 gtctgttaat tcattctatc tgactatata aactattgca agttacatag tttatcacca
     3901 cttggactat gtttatcacc gctaatgacg actttatcac cgcttacagc gtgtttatca
     3961 ccactcacgg cgattctgtc cattgtttac cgtttgttca ttcacccgta ataatttgtt
     4021 catactttcc ttttacaatg aaaccaacaa gtacatgaga ggggttttat ttatggcaaa
     4081 ttcattcgga aaacgtttcg tgtcgacagt cggggccggc attcttgggt ctgcgctaac
     4141 attgggcgtc gtggcgaaca cagacatctt acaggcaaaa acacagaagg cgcagacggc
     4201 tgctgtaacg gagagttctt cctcatctcc gaatttcgtc caaacttcgg cgaatgcccc
     4261 gacttccctg tccgacatgg tggagcatgc atcgaaaggg attgtcggtg tatcgaactt
     4321 taaaaatacc ggcaaccggt ttgcagggag ctcggagctt tcggaattcg ggacgggttc
     4381 cggcgtcatc tataaaatcg atggagacga cgcttatatc gtgacgaaca accatgtcat
     4441 tgaaggtgcg ggaaaaatcg aaattacatt gcatggcggg gagaaagaga cggcggaatt
     4501 gatcgggagc gatgcattga ccgatttggc agtgttgaag atcaatagta aacaggtcga
     4561 cacagccctt gaattcgggg attccgacaa attgcgtgct ggcgattcgg tcgtcgcaat
     4621 cggaaatccg ctgagtcttg atttctccgg aactgtcaca caaggcatca ttagtgcgcc
     4681 agcacgttcc atcgacgtga aaacgacagc aggaaactgg gaaatgaatg tcatccaaac
     4741 cgacgcggct atcaatcccg gcaatagcgg gggtgcactt ctgtcgacag agggcaaagt
     4801 gatcggcatc aacagtttga aaatcgcgga aaacgggatt gaaggcctcg gcttcgcaat
     4861 cccgagcaat gatgtcgttc cattgctcga acaaattacg gaaaacggaa aaatcgagcg
     4921 gccttatatc ggcgtcggcc tcgcggactt ggccgatgtt ccgtacatgt atgtacagaa
     4981 cttgccgcaa gaagtgaaag gcggcgtcat gatcgcaagc gtcgacccga catccgccgc
     5041 gggcaaggcg ggattgaagg agcaggacgt catcaccgcg attaacggaa ccgacatcct
     5101 caattcaatg gagctgcgaa aattccttta ttccgagctt aaaatcggag acaaagcgac
     5161 gctcaccgtt tattcaggct cccaaaaacg gacggttgaa ttgacgttga caagtaattc
     5221 cccggtcgaa taaatgaaac aaataaacac ctctgccatt tcaacacggc tgaggtgttt
     5281 atttatgatt taaccaagtc ttttgcttgc aaatagcttt cccctaaact cgtgatcgtc
     5341 gttccgcctc ttccttggcg ggcgttcaac agtcccagtt cttgcagaac ttcgagccgg
     5401 aggcgtattt gctgttcgga taaaataata tcatgctcct ccaaaagctt tttcagcttg
     5461 atccgtccga acgttttcag ctgcgctttc ccatcagcgt aaatgcttaa tatttgaatg
     5521 ctttccttca aaaatccatg ctcttcgatt tttttgtcaa tggagaggat ttcgttatga
     5581 gaaaggtttg cggcatccgc cagtgtttcc ctttcagaag caattcctgt tattcccggc
     5641 tctgcaagcg acacctgaga cttcacataa cgattcacca attgttcatg agtgaacccg
     5701 gcctccaatg cctccgcaat ctcccatacc gtcccgtagg atagaagtcg atcgccgata
     5761 tcgatgacat ttgaaaatct gctcggcacg aattccatct ctcccggcgt gacgatatag
     5821 tcgatatcct cggggatcgt ttgatcggga ttgttttcgt cgcccacata atacgtatgg
     5881 gtaaagaaga tattcatcaa cgagtccaac gtttcctggg cgttttcgag cgtatcgttg
     5941 acgaccaaca acttttgtcc cgccggcaga tcaatcaatc gcttcgtatt cgcgatattc
     6001 acttcccgat gcgcaatgat cgtcttgcaa tctttcggaa tgaagggccg gatgaccccc
     6061 atcagaatcg atcttgataa aacgacaatg tcctcttccg caatgctatc cccgtccaaa
     6121 tccttgacga ccaatttcga gattgttgct ttatcaccaa aaataagttc cagttgttct
     6181 gagattatcg ttaaataacc ttcttgtatc gagatgatat gaattttggt tttcatgctt
     6241 tctcctcatc ttccattatc cacccactaa tttcgcaact atcataccat ttactgggta
     6301 tatgaccaag cggatgcgct tcaatcaatc gatgagatgg caggcaaccc aatggtccct
     6361 ttcggcctct ttccatatcg gagtctcttt acatttctcc atcgcgtatg gacagcgcgt
     6421 ccggaaacgg catccggtcg gcgggttcat cggattcgga agatctcctg tcaagacgat
     6481 cctttccctt ccccgttcct ccacaggatc cggcaccggc acagccgaca tcaaagcctg
     6541 cgtataagga tgaagcggtt tttcgctgag cgccttcgta ggggcgagct cgacgagatt
     6601 ccccaagtac atgacaccaa tgcgatcgct aatatatttc accatcgaaa tgtcatgtgc
     6661 gataaataaa tacgtcaaat cattttcata ctgcaaatcc ttcagcaaat tcacgacttg
     6721 tgcctggatt gacacatcga gtgctgagat cggctcatcc gcaatgatca cgtcaggatt
     6781 cactgccaag gcacgcgcaa tgccgaccct ttgccgttgt cctcctgaaa attcatgcgg
     6841 ataccgattc atgaattcct tgttcagccc gacgatttca agcaagtttt ggacgatatc
     6901 cttcctgtct ttcggactgg cggccagtcc atggatatcg atgccttctg caacagagtc
     6961 gagcactttt ttgcggggat ccagtgaagc atacgggtct tggaaaatca tctggatctt
     7021 ttgcttcatc cgattttcct gccccttttt cagttgccca tgaatgggct cgccgtcgaa
     7081 gtacacttca ccgcctgtcg gttcatacag acggatgatt gttctgccgg tcgtcgattt
     7141 cccgcatccc gactccccga ccaaaccgag tgtctcccct ttatgaatat cgaaggaaac
     7201 gccgtcaaca gcgcgaagat gcttattccc cgatagatga aagtgcttcc gcaaatcttt
     7261 cacctggatg atcggggata aatcggcaat tctgcttttc ctctttttcg ctaccgccac
     7321 cctttccctt tctttcactt gaatgaccgt cgaaagatcg ggggctttcg gatgcagcag
     7381 ccaagttgcc gcggaatggc tgtcggacac tttgaacatc ggcggctggg tcttgtaatc
     7441 gatcgccaac gcaaattcgt tccgcacagc aaatgcgtct ccgacaggcg ggtgcaataa
     7501 atccggaggc gaacccggga tggagtatag ttcctcttcc gcatccaaat cagggatcga
     7561 tttcaacagt ccccatgtgt acgggtgctg tgattcatag aagacctcat tgaccgtacc
     7621 ctcttcaacg attttacctg catacatgac cgctactttg tccgccatat tcgctacgac
     7681 gcccaagtca tgggtgatca ggatgatcgc cgtgcccatc cgtttttgga tgtctttcaa
     7741 taaatccaag atctgcgcct ggattgttac gtccaatgcc gtcgtcggct catccgcaat
     7801 gaggagcctc ggattgcatg caatcgcaat cgcgatgacg acgcgctgcc gcatccctcc
     7861 cgaaaactga tgagggtact gattcacgcg ggtggctggc tccggtatgc cgaccagttc
     7921 caatatttcg atggcacgtt gcttcgcctc tttttttgat aaaccctgat gcttcatgac
     7981 actttccgcg acctgtttcc cgattgtcat cgtcggattc agcgccgtca tcgggtcttg
     8041 gaacaccatg gcgatttccg agccgcggac gacttccatc cgccgctccg gtgcttccag
     8101 cagattttga ccgtcgaaca ggacctgtcc gccatcatat gaagcgatcc tatcaggcag
     8161 cagtttcatg atcgctttgg atgtgaccga cttgcctgat cccgattcac ctacgatggc
     8221 caatgtctcc ccttcattta ttgtaaaatc gactcccctg acggctttta caactcccgc
     8281 ctgggtatgg aaagatactt gtaagtcttt cacttccagt aatggcttca ttgaaatccc
     8341 ccctcggatc aacacatcat tttttcattt tcggatcgag cgcatcccgc aggccatccg
     8401 ccaacaggtt gaagcagacc atcagtccga caatgaccaa agccgggaat agcaagagat
     8461 agaaatgatt tctcatcgcc tggaatccgt cgtttatcaa tacgcccaaa gatgcgagcg
     8521 gctcttgcag cccaaggccg atgaagctta gaaacgcttc cgtgaaaatt gctgcaggaa
     8581 tcgtgaacat catattgacg atgatgagac cgagcgtatt cggaatgaag tgctccttga
     8641 tgagcctagt attcgttgca cccaatgccc tcgaggcgag aatgaattcc tgacccttca
     8701 gcttgaacac ttgtgaacgg actagcctcg ccatgttcac ccatcctgta atgaccatcg
     8761 cgatggtgat cgtcacaatc cccggtttca ttaacataat gagcaagatg atcagaatga
     8821 gattcggaat gccgaccagc acttcaatga tccgctgcat cacattatcc acccggccgc
     8881 ctaacagtgc ggaaacagct ccatacataa ccccaatgat caagtcgagg gtagcggcca
     8941 agaatgcgat gtataacgag atcctcgtcc cttcccaaat ccgcgtccat tggtcccggc
     9001 caaaccggtc cgtcccgagc caatgctcgc cgctcggttt ctggaacact ttgtcgtaat
     9061 cggtttgctc ataggaatac tcattcaaat gcggtccgat gatcgccatg acgatcagga
     9121 tggacaggat gaccaagctg agcatcgccc ctttattttt cttaaggcgg atccagcttt
     9181 ccttgaacat gctcatttg
//
LOCUS       DASULI010000383         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_751991, whole
            genome shotgun sequence.
ACCESSION   DASULI010000383 DASULI010000000
VERSION     DASULI010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751991"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..873
                     /locus_tag="VFX34_08305"
     CDS             172..873
                     /locus_tag="VFX34_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5564948.1"
                     /translation="MDILVIEDNESVSAMIGLFFEKEGIRGEFVKDGLQGYERAVSGS
                     WDCLIIDWMLPGMDGVTICRKLRQAEVAVPIIMLTAKDSESDQVLGLEMGADDYVTKP
                     FSPLALMARIKAVTRRYGVAKEDADDVGILKTKHFTINTSTREVLLDGKPMPNLTPKE
                     FDLLHFFIQHPRQVFSREQLLDRVWGYDFYGDDRTVDVHIKRLRSKVSNADQPFFHTV
                     WGIGYRFDETTGEQE"
     gene            870..2291
                     /locus_tag="VFX34_08310"
     CDS             870..2291
                     /locus_tag="VFX34_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5564949.1"
                     /translation="MKIKYFHQQLASHFSVIIIAFLILSLLFSHFVEQFVYNNKTEEL
                     ATYGQSILTDIEQDQRNSRSILKSYGYVLESRDIQYSLFDEKSVIIYSTGLKTPLIEL
                     NEEEWRELQNGKTVTVKQEFKRFEQAVTFVLLPYFHRNQFVGGVLLTSPIKGSREVIS
                     QMNSYLIYTTFIALAIALLLSGLLSTFHGRRINRLRSATANVARGDYSVRIPTAGVDE
                     IGELAGDFNRMVEKLEAAAEEIESLENRRRQFLADVSHELRTPLTTIRGIIEGLRNDM
                     ISESEKERGLQLASSETMRLIRLVNENLDYEKIRSNQVTLVKQEIQLEELLEIIREQL
                     EDAAAERGDKIVVDVPSETSVFADYDRLTQVLINITKNSIQFTENGMIWLRGYSQDDL
                     TVIEIEDTGIGMDPGEIEKIWSRFYKAIDSRTTNPYGEFGLGLSIVKQLVTLHGGTID
                     VESVKGEGTKFRIELPNEVVGEE"
     gene            complement(2580..>2941)
                     /locus_tag="VFX34_08315"
     CDS             complement(2580..>2941)
                     /locus_tag="VFX34_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5564950.1"
                     /translation="DEAAEEHGFDRSNIVAIGYSNGANIAGSLLFHYADSLKGAILHH
                     PMVPRRGIGLPDLTGVPVFIDAGKNDPICPAQETVDLQELLSGAGASTEVHWEMNGHS
                     LTRTEVEAAAEWYQNNF"
BASE COUNT          824 a          699 c          727 g          691 t
ORIGIN      
        1 atttccgtaa catttaattt tttaattaaa tatttactat tcagtcataa atccattcct
       61 ctttagcatt ctagtaaaac atatatataa caagaggctt tatgttttcc gtgtatataa
      121 aagtctgtat aatttaaata aagaagaatt gcgtcggaag ggggacacca aatggatatt
      181 ctcgtcatcg aagacaatga aagcgtctct gccatgattg gactgttttt tgaaaaggaa
      241 gggatccggg gcgagtttgt gaaggacgga cttcaaggct atgaacgggc cgtatcgggg
      301 agttgggatt gcctgatcat cgattggatg ctgcccggga tggacggcgt gacgatctgc
      361 cggaagctgc gccaagccga agttgcggtg ccgatcatta tgctgacggc aaaagatagc
      421 gaatccgacc aagtgcttgg acttgagatg ggcgcggatg attatgtgac aaagccgttc
      481 agcccgcttg cactcatggc gcgcatcaaa gcagtcaccc gccggtacgg tgtcgcaaag
      541 gaagatgcgg atgatgttgg catattgaaa acgaaacatt tcacgatcaa cacgtcgacg
      601 cgagaagtgc ttttggacgg caagcccatg ccgaacttga caccgaagga attcgacctg
      661 ctccattttt tcattcaaca tccaaggcag gtcttttcgc gcgagcagct gttggaccgg
      721 gtatggggct atgacttcta cggcgatgac cgtacggtgg atgtgcatat caaacggctc
      781 cgttcgaaag tgtcgaatgc cgatcagccg tttttccata cagtttgggg catcggctac
      841 cggttcgacg aaacgacagg tgaacaggaa tgaagatcaa atacttccac cagcagctgg
      901 caagccattt cagcgttatc attatcgctt ttctcattct tagcctgctg ttctcccatt
      961 tcgtcgagca gttcgtctat aacaataaaa ccgaggagct cgccacatac ggacaatcga
     1021 ttttgacgga tatcgagcag gaccaacgaa attccaggtc catcttaaaa tcgtacggat
     1081 atgtattgga aagcagggat atccaataca gcttgttcga cgaaaagtcg gtcattattt
     1141 actcgaccgg gctgaagacg cccttgatcg aactgaatga ggaagaatgg cgggaattgc
     1201 aaaacgggaa aacggtcact gtgaaacagg aattcaaacg gttcgaacag gcggtcacgt
     1261 tcgtcctcct cccctatttc catcggaatc agttcgtcgg cggtgtttta ttaacctccc
     1321 cgatcaaagg ctcccgtgaa gtcatttcgc agatgaacag ctatttgatc tacacgacgt
     1381 tcattgcgct ggcgattgca ttgttgctga gcggacttct atcgacgttc cacggaaggc
     1441 gcatcaaccg tctgcgatca gctactgcga atgtggcgcg aggcgattat tcagtgcgca
     1501 taccgaccgc gggcgtcgat gaaatcggag agcttgcagg ggatttcaat agaatggtcg
     1561 agaagctcga agcggctgcg gaggaaatcg aaagcctaga aaaccgccgc cggcaattcc
     1621 tggcggacgt gtcccatgag ctacggacgc cactgacgac aatccgcgga atcatcgaag
     1681 gactgcgcaa tgacatgatc tccgaatctg aaaaggaaag gggcctgcaa ctcgcaagca
     1741 gcgaaacgat gcgtctcatc aggctcgtga atgaaaacct cgactacgaa aaaatccgtt
     1801 caaaccaagt gacattggtg aaacaggaga ttcaacttga agagttgcta gaaatcattc
     1861 gggaacaact ggaagatgct gcggcggaac gcggtgacaa aatcgttgtg gatgtcccgt
     1921 ctgaaacatc cgtcttcgcc gattatgatc gactcaccca agtgctgatc aatatcacga
     1981 aaaacagcat ccaattcacg gagaacggga tgatttggct gcggggttat tcccaggatg
     2041 atcttacagt cattgaaatc gaggatactg gcatcggcat ggatcctggg gaaatcgaga
     2101 agatttggag caggttctat aaggcgattg actcacggac gacgaatcca tatggcgagt
     2161 tcggtttagg attgtcgatt gtgaaacagc tcgtcacctt gcacggcgga acgattgacg
     2221 tggaaagtgt gaaaggcgaa gggacgaaat tccgcattga gctgccgaat gaggttgtgg
     2281 gcgaagagta atagagccct gcatggaata actgccaaaa aactacacct ccaattgtta
     2341 gagtgtatct aacatttggg gtgcagttca gttaagaaga gcggctttta gatacacaca
     2401 ttttattgta atcacgtata aatcctccga tacacgcata aaaccttgca aaagctcata
     2461 actcttcgca aaagcgcata actcgccata aaactccata aacgccgaaa acgacgcata
     2521 aatcatgata attgcgcata aatccaaaaa gccgccccat gcaggatcgg ctttttacgt
     2581 taaaaattat tttgatacca ctccgcagcg gcttccactt ctgttcgcgt caacgaatgg
     2641 ccattcattt cccaatggac ttccgtagaa gcgcccgctc ccgacaacaa ctcttgcaaa
     2701 tctacagtct cctgcgccgg acagatcgga tcattcttcc ctgcgtcaat gaacaccggc
     2761 accccagtca aatcaggaag gccgataccc cgtctcggca ccattggatg atgcaaaatg
     2821 gcgcctttca acgaatccgc ataatggaac agcaagctac ccgcaatatt tgcaccgttc
     2881 gaatagccga tagccacaat attcgaccgg tcaaatccat gttcttccgc tgcttcatcc
     2941 a
//
LOCUS       DASULI010000384         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1938441,
            whole genome shotgun sequence.
ACCESSION   DASULI010000384 DASULI010000000
VERSION     DASULI010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938441"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VFX34_08320"
     CDS             complement(<1..821)
                     /locus_tag="VFX34_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5564951.1"
                     /translation="MSTLQFLADGFATAFQWHNLLFAFIGVVIGTAVGVLPGIGPMSG
                     VALLIPVTATITSGMPTTAAAASSIILLAGVYYGAMYGGSTTSILLNTPGESSSVVTT
                     LDGYQMARQGRAGAALAIAAIGSFCAGIISLIGLVLLAEPLSNVAIKFGPAEYFSLML
                     LGLAAVSGLAGKSMTKALMMTVFGLMLGTIGIDAVSGIARFTYNQPILFSGLEFLTIA
                     VGLFALGEVFKTVLERDDEGGAIAKINRILPTKQDMKDSTVPIVRGSLLGFFIGVL"
     gene            complement(840..1292)
                     /locus_tag="VFX34_08325"
     CDS             complement(840..1292)
                     /locus_tag="VFX34_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5564952.1"
                     /translation="MSKTFDRYASIAFLLIGLLFVVESMKISSSAYGSTVGPKIFPMG
                     LGIILILLSLRLLYETFKNKAEATASAEKLQYKKFLIILVSAALYAFLLEKIGYVIST
                     FLFLLIAFQTMERGRIVPTLIIAAVFSFGVYYFFAEFLGGSLPGFPRF"
     gene            complement(1540..2553)
                     /locus_tag="VFX34_08330"
     CDS             complement(1540..2553)
                     /locus_tag="VFX34_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5564953.1"
                     /translation="MKWKKFKIAALASVVALSLAACSKTDGEEAGSSKYPERNITIVA
                     PSGAGGGWDLTARAIAKTMNDTKLIEKPITVENKPGGGGAVYMAEFATKEVKNDYALI
                     VKSPPLLINNNKAEGNSPYGYKDTTPLAQLTRDYGAIVVKADSPFKTLEDALKAIKKD
                     PRSVTLAGGSAPGSMDHLVGVLPAFEYGIDPKSVKYVSYDGGGEAVAALLGGNADLIA
                     TDASTIGQYVKSGDVRVLAVSSSERLGGELAEVPTFKEEGIDAEFTIWRGLFGPKEMT
                     DGAVDYWTAKLKEMVETDEWKAELERNGWESEYRGPEDFTKYLEDQDKVIVELLTALG
                     MQK"
     gene            complement(2693..>3078)
                     /locus_tag="VFX34_08335"
     CDS             complement(2693..>3078)
                     /locus_tag="VFX34_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX5564954.1"
                     /translation="ILKPLTFDRFKESLLSYKAKREKLTGEGVMKEDSIQFLWNKTNA
                     EPADNPESLPPKGIDPITKEKVVSHLKQVEGGITAETLGVEIGVSRSTARRYLEYLVS
                     EKRAYTELIYGSVGRPERRYFIQQR"
BASE COUNT          761 a          819 c          698 g          800 t
ORIGIN      
        1 aggactccga tgaaaaatcc aagcaatgaa ccgcggacaa tcggtacggt actgtccttc
       61 atatcctgct ttgtcggaag gatgcggttg attttcgcaa tcgcccctcc ttcatcatcc
      121 ctttccaata ccgttttgaa cacttcacca agtgcgaaaa gcccgacagc gatcgtcaaa
      181 aactccaatc cggaaaacag gatcggctgg ttgtatgtaa atcgggcaat tcctgaaacg
      241 gcatcgatgc cgatcgttcc aagcatcagt ccgaagacgg tcatcatcaa cgcctttgtc
      301 atcgattttc cggcaagacc gctaaccgct gccaagccta gcaacatcaa tgagaaatat
      361 tccgccggcc caaatttgat ggcgacattg gacagcggtt ccgcaagcaa aacaagaccg
      421 atcaaagaaa tgattccagc acagaatgac ccgatggcag ctatcgccaa tgcagcgcct
      481 gcacggccct gtcttgccat ctgatagccg tcaagcgtcg tgacgacgga agaggattca
      541 cctggcgtgt tcaatagaat ggaagtggtg gatcccccat acatcgctcc gtagtacacg
      601 cctgcaagca aaatgatgga actggctgcc gccgcggttg tcggcatgcc ggatgtgatc
      661 gttgctgtga ccggaataag gagcgcgacc ccgctcatcg ggccgatgcc aggcaacacg
      721 ccgacagcgg ttccgatgac gacgccgatg aaagcaaata atagattatg ccattggaag
      781 gcggtagcaa aaccgtcagc caaaaattgc aatgtgctca tagtgtgact cctttcttct
      841 taaaacctag ggaatccggg caatgagccg ccaagaaatt ccgcaaagaa ataatagacg
      901 ccaaatgaaa agactgcggc gataatgaga gttgggacaa tccggccgcg ttccatcgtc
      961 tggaatgcga taagtaaaaa gaggaacgtt gaaatgacgt acccgatctt ctccaatagg
     1021 aacgcataca acgctgcact tacgagaatg atgagaaact ttttatattg cagtttttcc
     1081 gcggatgccg tggcttccgc tttatttttg aacgtttcgt acagcagccg caagctcaat
     1141 aaaatcaaaa tgatgccgag ccccatcgga aagattttcg ggccgaccgt cgatccgtac
     1201 gcactgcttg aaatcttcat actttcaaca acaaataaaa gaccgatcag taaaaaagcg
     1261 atgcttgcat atcgatcgaa tgttttactc atagtctttg acacctcctt ataaaaaaag
     1321 cgaatggcgc ctgcatagag gcgacaggca taagacaaag atgtggcgtg gcgctttttg
     1381 ccacagaaca gctttgactt atgaccccga gacaatcgaa caacgaaggg ttcgatttgc
     1441 cgtttttctg cgatttctgc agaaaataag gcatcctctt tcagcgccag tagctggatt
     1501 gaaaaggcgg ccgttcaagc cgcctgcaca actttttcct tatttttgca tgccaagtgc
     1561 agtcagaagc tcaacaatga ctttgtcctg gtcttccaaa tacttcgtga aatcttccgg
     1621 accgcggtat tcgctttccc agccgtttct ttcaagttcc gctttccatt cgtccgtctc
     1681 gaccatttct ttcagttttg ccgtccaata gtcgacagct ccatcggtca tttccttcgg
     1741 tccgaaaagc ccgcgccaaa tcgtgaactc agcgtcaatg ccttcttctt tgaatgtcgg
     1801 cacttcagcc aattcaccgc ctagacgctc tgaagaactg acagcaagga cacgtacatc
     1861 gccggatttc acatattggc cgattgtcga tgcatctgtt gcaatgagat ccgcatttcc
     1921 gccgagcagc gctgcaacag cttctcctcc gccatcatac gaaacatatt tcactgattt
     1981 cgggtcgatt ccatattcga aagcaggaag gacgccgact aggtggtcca tcgagcctgg
     2041 tgcggatcca ccggcaagcg taaccgatct tggatctttc ttaatcgctt tcagcgcatc
     2101 ttccaatgtt ttgaatggtg aatccgcttt cacgacaatc gcgccgtaat cgcgtgtcaa
     2161 ctgtgccaat ggcgtcgtat ctttatagcc gtaagggctg tttccttcag ctttgttgtt
     2221 gttgatcaga agtggtggag atttcacgat caacgcgtaa tcattcttca cttctttcgt
     2281 tgcaaactcc gccatgtaaa cggcaccgcc gccacctggt ttattctcga ccgtgatcgg
     2341 cttttcgatc agcttcgtgt cattcatcgt tttcgcaatc gcacgtgcgg tcaaatccca
     2401 tccgccgcct gcccctgatg gtgcgacaat tgtgatattg cgctcaggat atttcgacga
     2461 acccgcttct tccccatctg tcttcgaaca tgctgcgagg ctcagtgcca caacacttgc
     2521 caaggcagct atcttgaact tcttccactt catcttgcat ccccctttgg aataaaaaaa
     2581 ttgatggcag cgctttcata cgctatcatc aatttatcag aaagttggtt gttttcaatg
     2641 tttgcggtca taagttcaat attgggcgta aacgtcattt tgttcatatt gttcaccgtt
     2701 gttgaatgaa ataccttcgt tccggccgac cgaccgaacc gtagatcagc tcggtatacg
     2761 cccttttctc tgaaacgaga tattccaaat accgcctggc agtcgagcgg ctgacaccga
     2821 tttccacgcc aagcgtttcc gcggtgattc ctccttcgac ttgctttaga tggctgacga
     2881 ccttctcttt cgtgatcggg tcgatccctt tcggggggag ggattccgga ttgtctgcag
     2941 gctcggcatt ggttttattc cacaggaatt ggatggaatc ctctttcatg actccttcgc
     3001 ccgtaagctt ctcccgtttc gccttgtagg aaagaaggct ttccttgaac cggtcgaatg
     3061 tgagcggctt caatatat
//
LOCUS       DASULI010000385         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_382851, whole
            genome shotgun sequence.
ACCESSION   DASULI010000385 DASULI010000000
VERSION     DASULI010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382851"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2208)
                     /gene="rpoC"
                     /locus_tag="VFX34_08340"
     CDS             complement(<1..2208)
                     /gene="rpoC"
                     /locus_tag="VFX34_08340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008497419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5564955.1"
                     /translation="MIDVNNFEYMKIGLASPDKIRSWSYGEVKKPETINYRTLKPEKD
                     GLFCERIFGPTKDWECHCGKYKRVRYKGVVCDRCGVEVTRQKVRRERMGHIELAAPVT
                     HIWYFKGIPSRMGLILDMTPRALEEIIYFASYVVVDPADTPLERKQLLSEKEFRLYRE
                     KYGSKFQALMGAEAIEQLLMAIDLDKETEMLKEELKTVQGQRRTRAIRRLEVVESFRN
                     SGNRPEWMVLEVLPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGA
                     PGIIVQNEKRMLQEAVDALVDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPNLKMYQCGLPKEMAIELFKPFVMKELVERGLAHNIKSAKRKIER
                     LHSEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPMLVEGRAIQLHPLVCTAYNADF
                     DGDQMAVHVPLSAEAQAEARLLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLEREG
                     SIGEGSIFSNMNEALIAYQTGHVHLHTRIAIQAGSLNNPTFTEEQNRKLLLTTIGKLI
                     FNEILPETFPYINEPTNHNLQIETPDKYFIDGTVNVKEHIQNAEIVLPFRKKILGNII
                     AEIFKKFHITETSRMLDRMKNLGFRYSTRAGITVGISDIVVLPNKDEILQEAQDKVDK
                     VTQQFRRGRITEEERYDRVISYWSNAKDVIQDKLMDSLENTNPIYMMSDSGARG"
     gene            complement(2315..>3880)
                     /locus_tag="VFX34_08345"
     CDS             complement(2315..>3880)
                     /locus_tag="VFX34_08345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX5564956.1"
                     /translation="VGTGMEHVSARDSGAAVIAKYHGIVEHVEAKEIRIRRIETVDDK
                     EVKGDLTIYRLENFIRSNQGTCYNQRPIVSVGDRVKPRDILADGPSMDKGELALGRNV
                     LTAFMTWDGYNYEDAIIMSERLVKDDVFTSIHIEEYESEARDTKLGPEEITRDIPNVG
                     EEALRNLDDRGIIRIGAEVRDGDILVGKVTPKGVTELTAEERLLHAIFGEKAREVRDT
                     SLRVPHGAGGIVLDVKVFNREDGDELPPGVNQLVRAYIVQKRKISVGDKMAGRHGNKG
                     VISRILPESDMPYLPDGTPIDVMLNPLGVPSRMNIGQVLEMHLGMAARNLGVHMASPV
                     FDGANEEDVWTTMEEAGMNRDGKTILYDGRSGEPFDSRVSVGVMYLIKLAHMVDDKLH
                     ARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTVKSDDVVGRV
                     KTYEAIVKGQSVPQPGVPESFKVLIKELQSLGLDVKMLTEEFEEIEMRDLDDDDDMQP
                     TDALNLMAEDQTV"
BASE COUNT          918 a         1022 c          875 g         1065 t
ORIGIN      
        1 accacgggca cctgagtcac tcatcatgta aatagggttt gtattttcaa gagagtccat
       61 cagtttatcc tggatgacgt ccttcgcatt gctccagtag gagatgacac ggtcataacg
      121 ctcttcctcc gtaatacgac cacggcggaa ctgctgcgtc actttatcca ctttatcttg
      181 tgcctcttgc aggatttcat ctttgttcgg caatacgacg atgtcggaaa taccgactgt
      241 gatacctgcg cgagttgaat atctgaaacc gaggtttttc atccggtcaa gcattctaga
      301 agtttccgta atatggaact tcttgaagat ttccgcaatg atgttaccaa ggattttctt
      361 acggaatgga aggacgatct ccgcattttg gatatgctct ttgacattca ctgtaccatc
      421 gatgaagtat ttatccggcg tttcaatctg tagattgtgg ttcgtcggtt cattaatgta
      481 cgggaacgtt tcaggaagga tttcattgaa aatgagcttc ccgatcgttg tcaataaaag
      541 tttgcggttc tgttcttctg taaatgtcgg gttgttcaag gacccagctt ggatggcgat
      601 tctcgtatgc aaatggacgt ggcctgtttg atatgcaatc aaagcctcat tcatattgct
      661 gaagatcgac ccttcgccga tggacccttc cctttccaac gtaaggtagt agttacccaa
      721 taccatatcc tgggatggag taacaaccgg ttttccatct ttcgggttca agatgttttg
      781 cgcagcaagc attagcaaac gcgcttcagc ttgggcttct gccgaaagcg gaacgtgaac
      841 agccatttgg tcaccgtcaa agtcggcgtt atatgccgta caaacgagtg gatgaagctg
      901 gatggcacgg ccttccacca acatcggctc aaatgcctgg attcccagtc tatgaagagt
      961 aggggcccgg ttcagtaaaa ccggatgctc tttaatgacg tcttcgagga catcccaaac
     1021 ttcggaatgc aaacgttcaa ttttacgttt tgcactcttg atgttgtggg caagcccgcg
     1081 ttcaaccaat tccttcatga cgaatggctt gaataattca atggccatct ctttcggaag
     1141 accacattgg tacattttca agttaggacc gacaacaata accgaacgac ctgaataatc
     1201 gacccgttta cccaataggt tttgacggaa gcgaccttgc ttccctttca acatatggga
     1261 gagggatttc aacggacgat ttcctggtcc agtaaccgga cgtccgcgtc gtccattatc
     1321 aacgagcgcg tcgacggctt cctgaagcat acgtttttca ttctgcacga taatgccagg
     1381 tgcgccgaga tcaagaagac gcttcaaccg gttgttacgg ttaatgaccc tccggtaaag
     1441 atcgttcaag tccgatgttg cgaaacggcc gccgtccaac tgtaccattg ggcgcagctc
     1501 aggaggaatg accggcaaca cttcaagaac catccattcc ggacggtttc cggagttacg
     1561 gaaagattcg acgacttcca agcgtctgat cgcacgtgta cggcgttgac cttgtactgt
     1621 cttcaattct tctttcagca tttccgtttc tttgtccaaa tcaattgcca tcagaagctg
     1681 ctcaatcgct tcagcgccca tgagcgcctg gaatttcgaa ccatactttt cacggtacag
     1741 tctgaattcc ttctcggaaa gcaattgttt ccgttcaagc ggcgtatccg caggatcgac
     1801 gacaacgtaa gatgcgaaat aaatgatttc ttccaaagca cgtggtgtca tatcgagaat
     1861 gagacccatt ctgcttggga tgcctttgaa ataccaaata tgcgtgaccg gtgctgcaag
     1921 ctcgatatgc cccatacgct cacggcgcac tttttgacgg gtcacttcga cgccgcatcg
     1981 atcacagact acgcctttgt aacggacgcg cttgtatttc ccacaatgac attcccagtc
     2041 ttttgtaggt ccaaagatcc gttcacagaa cagaccgtct ttctcgggct tcaacgtacg
     2101 ataattgatc gtttcgggct ttttcacttc accgtaagac caagaacgaa ttttatccgg
     2161 tgaagcaagg ccgattttca tatactcaaa attgtttaca tctatcaagg agcctacctc
     2221 cctcttatct tatctccgcg atggactctc atcagtatcg ttaaaaatag aggagcgttc
     2281 atagctcccc tatccaattg tgcaaattaa ttcatcaaac tgtttgatct tcagccatga
     2341 gattcaatgc atctgttggt tgcatatcgt catcatcatc aagatcgcgc atttcgattt
     2401 cttcgaattc ttccgtcagc atcttcacgt ccaacccaag gctttgaagt tccttgatca
     2461 atactttgaa tgattcagga acgcccggtt gaggaacgct ttgacctttg acaatcgctt
     2521 cgtacgtttt cacacggccg acgacgtcat ccgacttgac ggtaaggatt tcttgcaatg
     2581 tataagcggc accgtatgct tcaagcgccc atacttccat ctccccgaaa cgctgtccgc
     2641 cgaattgggc tttcccgccg agcggctgtt gtgtaacgag cgagtatggt ccagtcgaac
     2701 gagcatgcag cttatcgtcg accatatgcg caagcttgat caaatacatg acgccgacag
     2761 atacgcggct atcgaacggt tcacctgaac ggccatcgta aaggattgtc tttccgtcgc
     2821 ggttcatgcc cgcttcttcc attgtcgtcc agacatcctc ttcgttcgca ccgtcaaaga
     2881 ccggtgaagc catatgaaca cccagattac gggctgccat tcctaagtgc atttccaata
     2941 cctgtccgat gttcatacgg gatggtacac caagcgggtt caacatgaca tcgatcggcg
     3001 tgccgtccgg aaggtatggc atatccgatt caggcaagat gcgggaaatg acccctttgt
     3061 taccgtggcg tccagccatc ttatccccta cagatatttt acgcttctgt acgatatacg
     3121 cacgaacgag ctgatttacg cccggcggca attcatcccc gtcttcacga ttgaacactt
     3181 tcacgtccag aacgattccg cctgcaccgt gaggaacacg caatgaagta tcacgcactt
     3241 cacgcgcctt ttcaccgaag atggcatgaa gaagcctttc ttcagctgtc agctcggtta
     3301 cccctttagg cgtcactttt ccgacgagaa tgtcaccgtc gcgaacttct gcaccgatac
     3361 ggataatacc gcggtcatcc aagttgcgaa gggcttcttc cccgacgttc gggatatccc
     3421 tagtgatttc ttcaggtccg agtttcgtgt cccgggcttc cgattcatat tcttcgatat
     3481 ggatcgaggt aaagacatca tctttcacaa gacgttcact catgatgatg gcatcttcat
     3541 agttgtaacc gtcccatgtc atgaatgctg taaggacgtt gcggcctagc gccagttccc
     3601 ctttatccat ggacggtccg tctgcaagaa tgtcgcgcgg tttcacacga tctccgacgc
     3661 tgacgatcgg gcgttggtta tagcaggtcc cttggttgga acggatgaag ttctcgagcc
     3721 tgtaaatcgt caagtcgccc ttcacttctt tgtcgtccac cgtttcaatg cggcggatcc
     3781 ggatttcctt cgcctcgaca tgctcgacga ttccgtggta tttcgcgatt acagcagctc
     3841 cggagtcacg cgctgacacg tgctccatac ccgtaccgac
//
LOCUS       DASULI010000386         4583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_264922, whole
            genome shotgun sequence.
ACCESSION   DASULI010000386 DASULI010000000
VERSION     DASULI010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264922"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VFX34_08350"
     CDS             <1..1236
                     /locus_tag="VFX34_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5564957.1"
                     /translation="WLKRNDVLMGGELTGWPRLMNGDPDMIASVIQAKKAGKKIEGHF
                     PGASERTLARMKLLGADGDHEAMTADEVEARLLQGYAVTLRHSSIRPDLPDLLKGILE
                     KELDVFDHLMMTTDGSTPSFHIDGVMDKCIQVALDAGVKPIDAYLMASYNVARYYGID
                     DLHGVIATGRYATFNFLDDEMSPVPTDVLSKGKWLKRENAPTAQFKQVDWSLVPEFEP
                     DFDLDEKDFDFNDAVGIEMVNDVITKPYEVKLDLSADKLSNEHDESFLMLIDRNGKWR
                     VNSFIKGFANQVQGFASSYSNTGDIILVGKDKEAMLLAFKEMKRLKGGMVLVEDGEVA
                     ASIPLKIGGGLSAARMEVLIDQEVALKKSLAERGYKHGDAVYTFLFLQSTHLPYIRAT
                     QVGLYQVMKNEVIIPATER"
     gene            1246..2313
                     /locus_tag="VFX34_08355"
     CDS             1246..2313
                     /locus_tag="VFX34_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX5564958.1"
                     /translation="MKGKKGGWLLALAIMLLLLSACSKKERAGAEEIVPEPEEEEEVI
                     GEEPQEEGPLLYRAPFSGEPAEEEKKARPVLATINNHPHARPQSGISQADIVYEFMAE
                     GNVTRFLALFQSELPETVGPIRSARDYFIHLAKGLDAFYVAHGYSPDAQKLLANRYVD
                     NVNGMQYDGTLFKRSTDRKAPHNSYITGENILAAMEKLGASGEIAKVPPFAFHETAED
                     AKIEDSASMIVVRFSASQDFINTFSYDSGTETYNRMVNGILTTDNANGEQVSLSNIIV
                     MEADHRTIDSVGRQSVDIESGGKAILFQAGIVKELEWKNVEGFLVPMENGVPAKLIPG
                     KTWIHIIPTYSGISKSVTYTP"
     gene            2342..2659
                     /locus_tag="VFX34_08360"
     CDS             2342..2659
                     /locus_tag="VFX34_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEX5564959.1"
                     /translation="MQIDKIRSEQTDQLFKAILELKNLEECYMFFDDLATMSELQSLA
                     QRLEVAHALRQKKTYDRIQNETGASTATISRVRRCVDYGSGGYNTVLDRLYPELAENN
                     EKK"
     gene            2771..3463
                     /locus_tag="VFX34_08365"
     CDS             2771..3463
                     /locus_tag="VFX34_08365"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HEX5564960.1"
                     /translation="MDYKNWRHVFKVDPAKRLEDEELEQLAESGTDGIIIGGTDGVTL
                     ENVLELLARIRRFSVPVALEVSTVESVTMGFDYYFIPTVLNSTDPKWVNGIHHAALKE
                     YGHMMDWDEVALEGYVIVNTDCKAARLTGVNEAPDEEDVIAYARMAEHLFKLPVFYLE
                     YSGTLGDPAIVKAAARILEKTRLVYGGGIRNAKDAKMMAELADTVIVGNIIYEDLKAA
                     LSTVEAVKSVSH"
     gene            3518..>4583
                     /locus_tag="VFX34_08370"
     CDS             3518..>4583
                     /locus_tag="VFX34_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5564961.1"
                     /translation="MKEIAENLLAGMNPEQARAVKKTEGPLLIMAGAGSGKTRVLTHR
                     IAYLIVEKGVYPSNVLAITFTNKAAREMRERIDGLLGPGSGERMWVSTFHSMCVRILR
                     RDIDRIGISKSFTILDSSDQLTAIKNVLKERNIDPKQYDPRAMLGAISSAKNECIDAD
                     TYKGQINPHNPYEKTIADVYEGYTKKLRQNQSLDFDDLIMMTLKLFERVPEVLEFYQN
                     KFQYIHVDEYQDTNNAQYRLVKMIADKFKNICVVGDSDQSIYRWRGADIGNILSFEKD
                     YQNAEVILLEQNYRSTKRILQAANDVISNNRSRYDKNLRTENDEGEAIYVYKANDEKD
                     ESQFVVGKILELQKEENLKLD"
BASE COUNT         1291 a         1014 c         1283 g          995 t
ORIGIN      
        1 tggctgaagc gtaatgacgt cctcatgggc ggtgaactga caggatggcc gcgccttatg
       61 aacggcgatc cggatatgat cgcgtctgtc atccaagcga aaaaagcggg gaaaaagatt
      121 gaaggccact tcccgggagc atccgagcgg acgcttgcga ggatgaagct gctcggagcg
      181 gacggggacc atgaagcgat gacggctgac gaagtggaag cccgcctatt gcaaggatat
      241 gcggttacac ttcgccattc atcgatccgg ccggatttgc ccgatttatt gaaaggcatt
      301 cttgagaagg agttggacgt gttcgaccat ctcatgatga cgacggacgg ctcgacgcct
      361 tccttccata tcgatggcgt catggacaaa tgcatccaag tggcgctcga cgccggggtg
      421 aaaccgattg atgcatattt gatggcttcg tataatgttg cgcgctacta cggcatcgac
      481 gatctccatg gcgtcattgc gacagggaga tacgcgacgt tcaatttcct ggacgatgaa
      541 atgagtcctg tgccgacgga cgtcctgtcc aaagggaaat ggctgaaacg agaaaacgcg
      601 ccgacagcgc agttcaagca agtcgattgg tcgctcgttc cggaatttga acctgatttc
      661 gatctggatg aaaaggattt tgatttcaat gatgcagtcg gaatcgaaat ggtgaatgac
      721 gtcatcacga agccgtatga agtgaagctt gacctgtcag cggacaagct ttcgaatgaa
      781 catgatgaaa gcttccttat gttgattgac agaaacggga agtggcgggt gaattcattc
      841 atcaaaggct ttgcgaatca ggtgcaaggc tttgcatcct cctattccaa tacgggtgac
      901 atcatccttg tcgggaagga taaggaagcg atgctgctcg cgttcaagga aatgaaacgg
      961 ctgaagggcg gcatggtgct cgtggaagac ggtgaagttg ccgcatcaat ccctttgaaa
     1021 atcggagggg ggctgtcggc agcgcggatg gaagtgttga tcgatcagga agttgcactc
     1081 aaaaagtcgt tggcggaacg gggttacaag catggagatg cggtctacac attcctgttt
     1141 ctgcagtcga cccatttgcc atacatacgg gcgacgcaag tggggcttta tcaagttatg
     1201 aaaaacgaag taatcattcc ggcgacggaa agataggagg aacggatgaa agggaaaaag
     1261 ggaggatggc tgcttgcctt agccatcatg ttgctgctgt tgtcagcgtg ttcaaaaaaa
     1321 gagagagccg gcgcggaaga aattgtaccg gagccggaag aagaggaaga agtcatcggg
     1381 gaagagcctc aggaagaggg gccgctcctt taccgggcgc cattctccgg tgaacctgca
     1441 gaagaggaga aaaaagcgag gccggtgctt gccaccatca acaaccatcc gcatgcaagg
     1501 ccgcaatcgg ggataagcca ggcggatatc gtctatgaat tcatggcgga agggaatgtc
     1561 acgaggtttt tagcgttgtt ccaaagcgag ctgccggaga cggttggccc gatccggagc
     1621 gcgcgtgact atttcataca tctggcaaaa gggctggatg cattttacgt agcgcacggc
     1681 tacagccctg atgcgcagaa gctattggcg aatcgatatg tcgacaatgt gaatggcatg
     1741 caatatgacg gaacgttatt caaaagatcg accgatcgca aagctcccca taattcgtat
     1801 ataaccggcg agaacattct agccgccatg gagaagctcg gtgcatccgg cgaaatcgca
     1861 aaggtccctc catttgcttt ccacgaaacg gcagaggatg ctaaaataga ggatagcgca
     1921 tcgatgatcg tcgtccgctt cagcgcaagc caggacttca tcaacacgtt ttcctacgat
     1981 tccgggacag agacatataa ccggatggtg aatggaatcc tgacgacgga taatgcgaac
     2041 ggggaacagg tcagcttgtc caacatcatt gtcatggagg cggaccaccg tacgatcgac
     2101 agcgtcggca ggcaatcggt cgatatcgaa agcggcggca aagcgatcct attccaggcc
     2161 ggcatcgtta aagaactgga atggaaaaac gttgaaggct tccttgtacc gatggaaaac
     2221 ggagttcccg caaaattgat tcccggcaag acgtggatcc atatcatccc gacgtattcc
     2281 ggaatttcaa aatctgttac gtacacgcct taaatagtac tgaaagaaag aggggggtca
     2341 catgcaaatc gataaaatcc gcagcgaaca aacggatcaa ctgttcaaag cgatattgga
     2401 attgaagaac cttgaggagt gctacatgtt cttcgatgac cttgcgacga tgagcgagct
     2461 gcaatcgttg gcacagcgtc tggaagtcgc gcatgcattg aggcagaaga agacgtatga
     2521 ccggatccaa aatgaaactg gtgcgagcac agcgacgatc tcacgtgtga gacgctgcgt
     2581 cgattacggt tctggcggct ataacaccgt gttggaccgc ctctatccgg agcttgcgga
     2641 gaataatgag aagaagtgat cgggcggaga agcaatccgc ccgttttttt tggtatactg
     2701 gggaatagca gacgctcatt tggaaagaag cgtattcaaa atgcatttga agaagtaggt
     2761 ggttgacaaa atggactata aaaactggcg ccacgtcttt aaagtggacc ccgcaaaacg
     2821 attggaagac gaagagcttg aacagctcgc agaatccgga acggacggca tcatcatcgg
     2881 aggaacggat ggcgtgacac tggaaaacgt gcttgagctg ctggcgcgta tccggagatt
     2941 ctcagtgcct gtggcgcttg aagtgtcgac cgtcgaatcg gtgacgatgg gcttcgatta
     3001 ttacttcatt ccgacagtgt tgaactcgac cgatccgaag tgggtgaacg gcatccatca
     3061 cgcggcgttg aaggaatacg gccatatgat ggattgggat gaagtcgcct tggaaggcta
     3121 tgttatcgtg aacactgatt gcaaagcggc gaggcttacc ggcgtgaacg aagcgccgga
     3181 cgaagaggac gttatcgctt atgcacggat ggcggagcat ctgttcaagc tgccggtgtt
     3241 ttatttggaa tatagcggaa cgctcggaga ccctgccatc gtcaaagctg ctgcacgtat
     3301 ccttgaaaag acgcgtctag tttacggcgg cggaatccgc aatgccaaag atgcgaaaat
     3361 gatggcggag cttgcagaca cggtcattgt cggcaatatt atctatgaag atctgaaggc
     3421 cgctctttcg acggtcgaag cagtgaaatc ggtatcccat tgatgggatt tcatgtataa
     3481 tgaagaacaa atgttctttg gaaggcggga ttacgttatg aaggaaatcg cggaaaatct
     3541 gcttgcaggc atgaaccctg aacaagcccg cgcagtgaaa aagacggaag ggccgctgtt
     3601 gatcatggca ggcgcaggat ccgggaagac aagagtgctc acccaccgga tcgcttactt
     3661 gatcgttgaa aaaggcgtgt acccttccaa tgttttggcg atcacattta cgaacaaggc
     3721 ggcccgggaa atgcgcgaac ggatcgatgg cctgctcgga cccgggtccg gcgaacggat
     3781 gtgggtgtcg acattccact cgatgtgcgt ccgcatctta cggagggata tcgaccgcat
     3841 cggaatttcg aaaagcttca cgatcctcga ttcctccgac caattgacgg ccatcaaaaa
     3901 tgtgctgaag gaacgcaata tcgatccgaa gcaatacgac ccgcgggcaa tgctcggggc
     3961 aatcagttcg gcgaaaaatg aatgcatcga cgcggatacc tataaaggac agatcaatcc
     4021 gcacaatccg tatgaaaaga cgattgcaga tgtctatgaa ggctatacga aaaaacttcg
     4081 acaaaaccaa tcgctcgatt ttgacgacct catcatgatg actctcaaat tattcgaacg
     4141 tgtccctgaa gtcctcgaat tttatcaaaa taaattccaa tatatccatg tcgatgaata
     4201 tcaagatacg aataacgccc agtacagact tgtgaagatg attgcggaca agtttaaaaa
     4261 tatttgtgtt gtcggcgact ccgatcagtc gatttataga tggcgcgggg cggatattgg
     4321 caacatttta tcattcgaaa aagactatca aaatgcggaa gtcattttac ttgagcaaaa
     4381 ctatcgttcg acgaaacgga ttcttcaagc agcgaatgac gttatttcaa acaatcgaag
     4441 ccgttacgat aaaaatcttc ggaccgaaaa cgatgaaggt gaagccattt acgtttacaa
     4501 agcgaacgat gaaaaagatg aatcacaatt cgttgttgga aagattctcg agctgcaaaa
     4561 agaagaaaat ttaaagctcg atc
//
LOCUS       DASULI010000387         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_459514, whole
            genome shotgun sequence.
ACCESSION   DASULI010000387 DASULI010000000
VERSION     DASULI010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459514"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFX34_08375"
     CDS             <1..694
                     /locus_tag="VFX34_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX5564962.1"
                     /translation="ESRLQAIKEFTELGSGFKIAMRDLSIRGAGNLLGSQQHGFIDSV
                     GFDLYSQMLQEAIEEKQTGIVKSEIQDVEISLPVNAYLPDSYIKDGFQKIQMYKRVKA
                     IESEEDYSELVDEMTDRFGDLPLEADLLLRVGRIKAWGRIAGVESIKKQRSIIEIRLT
                     EEGTAKTDGAKIVADSMEFGRAVGFTMEDGRLIITVDERHTGKKTEFDVLEAMMRILY
                     SSIKESIPTDSV"
     gene            783..1319
                     /gene="spoVT"
                     /locus_tag="VFX34_08380"
     CDS             783..1319
                     /gene="spoVT"
                     /locus_tag="VFX34_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HEX5564963.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDRDGEV
                     ILKKYSPISELGQFAKEYAETLYETLGTPALISDRDEMIAVSGLSKKDYLNRQLAPDV
                     EDILKGRTIVTEKLEKAVEWVPGQVEQVKSYCIAPIVAGGDTIGAVYLLSKVHFIGEA
                     EQKAAETAAHFLAKQMEQ"
     gene            1483..1749
                     /locus_tag="VFX34_08385"
     CDS             1483..1749
                     /locus_tag="VFX34_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564964.1"
                     /translation="MGALSKNVKEELANALNDQNATMFVVDGKLFSLEVHETSFMDQE
                     DGNLAQQIEAFPELKESLQRYYDNPDMPRYTAKELKELRHERRK"
     gene            1733..2083
                     /locus_tag="VFX34_08390"
     CDS             1733..2083
                     /locus_tag="VFX34_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5564965.1"
                     /translation="MREENNRSYSVHFTEEFNKCLDQIQAFFAEQGEDVLEWWFSKED
                     NMIDEIDRLLSSFPYAGRTVEHGPFEGYRCLIYGKSRHRMLSYVIFYVVEEGKKEISV
                     INILPSHSKRQRIK"
BASE COUNT          688 a          438 c          551 g          541 t
ORIGIN      
        1 ggaaagccga ctgcaagcga tcaaggaatt cacggaactc ggatccgggt tcaaaatcgc
       61 gatgcgggac ttatcgatcc gcggagcggg gaacttactt ggctcccagc aacacggctt
      121 tatcgactct gtcggttttg atttatactc acaaatgttg caagaggcaa tcgaggagaa
      181 gcagacgggc atcgtaaaat cggaaatcca ggatgtcgag atttccttgc cagtcaatgc
      241 ttatttgccg gattcatata tcaaggacgg attccaaaag atccaaatgt ataaacgggt
      301 gaaagcgatc gaaagcgaag aggattactc cgaacttgtt gatgaaatga cggaccgctt
      361 cggcgatttg ccgttggaag cggatctgtt gctgcgtgtc ggaaggataa aagcgtgggg
      421 gcgcatcgca ggcgtcgaat caattaaaaa gcaacgttcc atcattgaaa tccgcctaac
      481 tgaagaaggg acggcgaaga cggacggcgc taaaattgtc gctgactcaa tggaattcgg
      541 cagggctgtc ggctttacga tggaggacgg ccgactgatc atcacagtgg atgagcgcca
      601 tacaggcaaa aagacggaat ttgacgtcct tgaagcgatg atgaggattt tatattcatc
      661 catcaaagag tccattccca cagattccgt ctaaaggaac tgttgcataa aatgtccaag
      721 catgatgcat actatatccg aacggaaaac ggttttaatt tttggaaagt gaggcatcat
      781 gcatgaaagc aacaggaatc gtgcgcagaa ttgatgatct gggcagggtg gtcatcccga
      841 aagagatcag acggacgctt cgcatacgcg aaggagatcc gcttgaaatc ttcaccgaca
      901 gggatggcga ggtcatttta aagaaatatt cccctatttc cgagctcggt caatttgcga
      961 aagagtacgc agaaacacta tacgaaaccc tcggaacacc cgccctgatc agtgacaggg
     1021 atgaaatgat cgccgtatcc ggattatcga aaaaagatta tttgaaccgc caattggctc
     1081 cggacgtgga agatatcctc aagggtcgta ccatcgtcac ggaaaagctt gaaaaggcag
     1141 tagagtgggt acctggacaa gtcgagcaag tgaaatccta ctgcatcgcc ccaatcgtag
     1201 cgggcggaga cacaattggc gccgtctatt tgctatcgaa agtacatttc atcggagaag
     1261 ccgaacaaaa agcggcagaa accgccgcgc attttttggc gaaacagatg gaacaatgaa
     1321 aacagcctgc acgcaccggg aagaattggt gcgggtgggc tgtttttgtt tgccggcaat
     1381 cgaaatggaa taggggaatc gtcgagtggg gattctgaat agtcaataac gtctattttc
     1441 tgtgctataa ttaaaaaaag aaatctattg aggtgatgca taatgggtgc attaagtaaa
     1501 aatgtgaaag aagaacttgc gaatgccttg aatgatcaaa atgcaacaat gtttgttgta
     1561 gatggaaaac tgttttcatt agaagttcat gaaacgtctt ttatggatca agaggacggt
     1621 aacttagcac agcaaattga agcattccca gagttaaagg aatccctcca acgttattat
     1681 gataatcccg atatgccacg ctatactgcg aaagaactaa aggaattgcg gcatgagaga
     1741 agaaaataac cgaagttatt cggttcattt cacagaggaa ttcaataaat gcttagacca
     1801 aattcaggct ttctttgcgg aacaaggaga agatgtgctt gaatggtggt tttctaaaga
     1861 agataacatg attgatgaaa ttgatcgtct gttatcttct tttccgtatg ccggcagaac
     1921 ggttgagcat ggaccttttg aaggctaccg ttgcctcata tacggaaaaa gtcggcatcg
     1981 aatgctaagt tatgttattt tttacgtggt tgaggaagga aaaaaagaaa ttagtgttat
     2041 taacattctt ccatcacatt caaagcggca acggataaaa tagtccaagg gggttaagat
     2101 tgaaatccca tttttcgccg ccttctcctt caaaacacag tcctaattca gttagaaaaa
     2161 cccctatgtt aaacagtctt caccaattaa caagaagggg tttttcgtta cttttcta
//
LOCUS       DASULI010000388         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_303838, whole
            genome shotgun sequence.
ACCESSION   DASULI010000388 DASULI010000000
VERSION     DASULI010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303838"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFX34_08395"
     CDS             complement(<1..578)
                     /locus_tag="VFX34_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEX5564966.1"
                     /translation="MKEGRTDMRFLILMYVLAFILLWEWLLPVIELTDTEYIAVFLYF
                     IAFSFLFGLLRMRWWLSIPLKVIYLFWSLHYVFFQTAILSRETVSLLINDLLSNFGII
                     AAWNWEDITNPFRTVLFFVLLWMTIYLIRHWIEVRKSILLFYAMTVVFIAVLDTFSSY
                     SAEGAIFRIMVSGLLLIGLLTISRISDRHGRK"
     gene            complement(578..1783)
                     /locus_tag="VFX34_08400"
     CDS             complement(578..1783)
                     /locus_tag="VFX34_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5564967.1"
                     /translation="MKKRKDMLSVTGRFVFIVGLFFSVYVFAMFQGGKVSWTIFYMLT
                     PFLLYSIALFAYPMNNIKAERIIRTKTVESGGKLAVTVKLTRTFPFPLLYTVAIEKWA
                     NPAMAKSAKDRTKHIFVFGFKKSVEFSYEIDKLPRGEHIVEGVEMEVSDFFGWIQKRV
                     ILELKDAILVYPNTTSIKYVPINAQYDRGSLVSPFSLVKDTTMATGVRDYQAGDRVTW
                     IHWKSFARTQNLMTKEFEDRQSEDIIVLLDGRDSELFEEKVELAASIVEEASNHQANI
                     AFASAGADTVAFPFIHSADQLHEVFVHLAKIKPVASEDVQPSSSALSAVAGSVVLVTA
                     DPDWTFIQSMVGLVNSTKSVICFVLVEGESTLPMQLQEQIRYAKAKGVTIHALSRKQF
                     SEAFQEVAS"
     gene            complement(1780..2730)
                     /locus_tag="VFX34_08405"
     CDS             complement(1780..2730)
                     /locus_tag="VFX34_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5564968.1"
                     /translation="MTYAQVIDKVLANIDEVMIGKRDIAELSLTALLAGGHVLLEDVP
                     GVGKTMMVKALAKSIGASFKRIQFTPDLLPSDVLGVSIYNPKEMEFEFRPGPIVGNIV
                     LADEINRTSPKTQAALLESMEESSVTVDGETIRIPQPFFVMATQNPIEYEGTYPLPEA
                     QLDRFLFKLKMGYPKRSEEIEVLSRAEKSVPIDHLESVLTIQELAELQHAVKEVNVDH
                     TIKMYIVNCAVETRNHPHVYLGVSPRGSLALMKSCQAYALIKGRTYVTPDDVKYLAPF
                     VFGHRMILRSEAKYEGITANEIVERILTRVSVPIDRVGSK"
     gene            complement(2952..>3545)
                     /gene="nadE"
                     /locus_tag="VFX34_08410"
     CDS             complement(2952..>3545)
                     /gene="nadE"
                     /locus_tag="VFX34_08410"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEX5564969.1"
                     /translation="FIAVRLPYGKQFDEDDARDALDFIKPSLLYTVDIKGAVDASEKA
                     LAKAGIIISDYTKGNEKARERMKVQYSIAASHSCVVLGTDHAAEALTGFYTKFGDGAA
                     DLMPIYRLNKRQGRQLMKELGSPVHLYEKVPTADLEDDKPALPDEVALGVTYEEIDDF
                     LEGKQVSDKALETIKKHYERSAHKRHMPVTIFDDFWK"
BASE COUNT          919 a          941 c          784 g          901 t
ORIGIN      
        1 attttccgtc catgcctgtc ggaaattctg gaaatcgtca acagtccaat taataaaagc
       61 cccgatacca taattctgaa aatcgcgccc tccgccgaat aggagctgaa tgtatctaaa
      121 acggcgataa agacaaccgt catggcgtag aaaaggagaa tgctttttcg aacctcgatc
      181 caatggcgga tcaagtagat cgtcatccat aacaggacaa agaaaagaac cgtccgaaac
      241 gggttcgtaa tgtcctccca attccaagcc gcgataatgc cgaaattcga cagtagatca
      301 ttgattaaca aggaaacggt ttctctcgag agaatggcag tttggaaaaa cacgtaatga
      361 agcgaccaaa aaagatagat gaccttcaac ggaatggaga gccaccatct catcctcagc
      421 aatccgaaca ggaaggaaaa cgcgatgaaa taaaggaaca ccgctatata ctctgtgtcg
      481 gtcaattcga tgacgggcaa aagccattcc cagaggagga tgaaggcgag cacatacatg
      541 aggatcagga aacgcatatc cgttcttcct tctttcatca actggccacc tcctgaaatg
      601 cctccgaaaa ttgctttctg cttaatgcat gaatcgtgac cccttttgcc tttgcgtacc
      661 tgatttgctc ctgaagctgc attggcaatg tgctttctcc ttcaacgagc acaaaacaga
      721 tgactgattt cgtgctattc acgaggccca ccatcgattg gatgaatgtc caatcgggat
      781 ctgccgtcac caggacgaca ctgcctgcaa ccgctgaaag cgcggacgac gacggctgca
      841 cgtcttccga tgcgacgggc tttattttag ctaaatgtac aaatacctca tgcaactggt
      901 cagcagaatg gatgaatgga aatgcgaccg tatccgcccc ggctgaagcg aaagcgatat
      961 tcgcctgatg gttggatgct tcctcgacaa tggaagctgc aagttcaacc ttttcttcga
     1021 acagctccga atccctgccg tcgagcagaa cgataatatc ctccgattgc cgatcctcga
     1081 attccttcgt catcaagttt tgcgtacggg cgaatgattt ccaatggatc cacgtgaccc
     1141 ggtcccccgc ctggtaatcc ctcacgcctg tcgccatcgt cgtatccttg acaagtgaaa
     1201 atggggacac gagggaaccg cggtcatatt gtgcattgat cggcacgtat ttgatggaag
     1261 tcgtattcgg ataaacaaga atagcatcct tcaactctag aattacgcgt ttttgaatcc
     1321 acccgaaaaa atcggaaact tccatttcta cgccttccac aatatgctcc cctctcggaa
     1381 gcttgtcgat ttcatatgaa aactccacac tttttttgaa gccgaatacg aatatatgct
     1441 tcgtgcgatc tttcgcactt ttggccattg ccggattagc ccatttttca atcgcgacgg
     1501 tatagagcaa agggaatggg aacgtccgtg taagcttcac cgtaacagca agcttccctc
     1561 cgctctcaac cgttttcgtc cggatgatgc gctcggcttt tatattattc atcggatagg
     1621 caaatagagc gattgaatac aagaggaagg gtgtcagcat gtaaaagatc gtccagctca
     1681 ctttgccgcc ttggaacatc gcaaaaacat agacggaaaa gaaaaggccg acgatgaata
     1741 cgaagcggcc tgttaccgat agcatatctt ttcttttttt catttcgatc cgacccggtc
     1801 gatcggtacg ctcacccggg tgaggatccg ttcgacgatt tcattcgcgg tgatcccttc
     1861 atacttcgct tccgaacgga ggatcatccg atggccgaac acaaaaggag ccaaatattt
     1921 cacgtcgtcc ggcgtcacgt acgtccgccc tttaataagc gcgtatgctt ggcaggattt
     1981 catgagcgca agcgacccac gcggactgac gccaaggtag acgtgcggat gattgcgcgt
     2041 ttccactgca caattgacga tatacatttt gatcgtatga tcgacattca cttctttcac
     2101 tgcatgttgg agttccgcca attcctggat ggtaaggacg gattcgagat gatcaatcgg
     2161 caccgatttt tccgcgcggg atagcacttc aatctcttcg ctccgctttg gataccccat
     2221 cttcaacttg aagaggaacc ggtccaattg cgcttccggc aacggatacg tcccttcata
     2281 ttcaatcggg ttctgggtcg ccatgacgaa aaacggctgc ggaatccgga ttgtttcccc
     2341 gtcgaccgtc acagacgatt cctccatgct ctcgagtaaa gccgcttgcg ttttcggtga
     2401 cgtccggttg atttcatcgg ccaaaacgat gtttccgaca atcggacccg gtctgaattc
     2461 gaactccatc tcctttggat tgtagatgga gacgccaagg acgtccgatg gcaataaatc
     2521 cggcgtgaat tggatgcgct tgaaagatgc gccgatcgat ttcgccagcg ctttcaccat
     2581 catcgtcttc ccgacgccgg gtacatcctc cagcaggacg tgtccgcctg caagtaaggc
     2641 agtcaggctc agctccgcta tatcccgctt gccgatcatc acctcatcaa tatttgcgag
     2701 aactttgtcg ataacttgtg cgtaggtcat tcttcttcct cccattccag cttctctcta
     2761 taacatgccc aaaattttac aacaacatgt ccgaaaattt acaaaatgta actttatagt
     2821 accttaataa tcagtcggct acaacctctt gaaaacacta ttaaacaaga attgacaaaa
     2881 acacacgaaa aaacagatga aggaaagctt cacgcattcc ctcatctgtc gtattgatag
     2941 tcctctattt ttcatttcca aaaatcatca aatatcgtga ccggcatatg acgcttatgc
     3001 gccgatcttt cataatgctt tttaatcgtt tcaagcgctt tgtccgaaac ctgcttccct
     3061 tccaggaagt catcgatttc ctcatacgtc acgccaagcg caacttcatc gggcagcgca
     3121 ggtttatcat cttccaaatc agcggtcggc accttttcgt acaaatgtac aggggatccg
     3181 agctccttca tcagctggcg tccctgcctt ttattcaacc ggtagatcgg catgaggtcg
     3241 gctgcgccat ctccaaattt cgtgtagaag ccggtcagcg cttccgctgc atggtcggtc
     3301 ccgagaacga cgcagctatg cgacgccgca atggagtatt ggactttcat ccgctcccgc
     3361 gccttttcat ttcctttcgt gtaatcggaa atgatgatgc ccgcctttgc caatgccttt
     3421 tcgctcgcat cgaccgcgcc tttgatgtcg actgtataaa gcaaagatgg cttgatgaaa
     3481 tcaagggcgt cccgcgcatc gtcttcatcg aactgcttcc cataaggaag tctgacggcg
     3541 atgaa
//
LOCUS       DASULI010000389         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1864343,
            whole genome shotgun sequence.
ACCESSION   DASULI010000389 DASULI010000000
VERSION     DASULI010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864343"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..2104)
                     /gene="pepF"
                     /locus_tag="VFX34_08415"
     CDS             complement(284..2104)
                     /gene="pepF"
                     /locus_tag="VFX34_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX5564970.1"
                     /translation="MVKSLPPRSAVNVEETWNLEDLFKTEEEYNAAIEQLESEVASFE
                     ERFKGKITDEKVILEALDGYASIMGKMVPIGTYTSLSHSVDQTNDEAQMRASKYGSIA
                     SKLGSQLSFFTSELSEVPSELLENAMNQSEEYKNYLEELIRQKPYKLHPEVEKTLAAF
                     SSVLNAPYGLYNTTKLVDMNFDDFEVDGEKYPLSYTTFEGGWESETDTKKRRAAYDAF
                     HAKLREYQHTTAKTYDMHLQKEKTTSDLRGYDNVFDYLLFNQEVDRSMYDRQIDLIME
                     HLAPHMRKYAKLLQKVHGLDEMTFSDLKISLDPNYEPKITIEESKKYINDALAIMGDD
                     YMNMIDRSYKERWTDFAQNAGKSTGAFCSSPYGVHPYILISWTGSMEDVFVLAHELGH
                     AGHFYNAHQHQNIFNSRPSMYFIEAPSTMNEMLMANHLLSNSDDPKFKRWVISSIVAR
                     TYYHNFVTHLLEAAYQRKVYERIDAGGSVNANVLNSLKRGVLEEFWGDDVKINEGAEL
                     TWMRQPHYYMGLYPYTYSAGLTISTQVSKRILEEGQPAVEDWLKVLQAGGTKSPAELS
                     KMAGVDITTEQPLLDTIAYIGELIDQLVELTEEIEAAQVN"
     gene            complement(2215..2514)
                     /locus_tag="VFX34_08420"
     CDS             complement(2215..2514)
                     /locus_tag="VFX34_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014644564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564971.1"
                     /translation="MEFRNIDEQIIQKYREDETLMIRLFVQWCLNNELDPHALYKKAY
                     PYQMENAALQEVIESDDGFDDLHIDNETMLDVLQLFGNEDLAFVVTDEIGRLKRK"
     gene            2576..2992
                     /locus_tag="VFX34_08425"
                     /pseudo
     CDS             2576..2992
                     /locus_tag="VFX34_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010529245.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
BASE COUNT          817 a          654 c          667 g          862 t
ORIGIN      
        1 agagtacggc aaaaccgaag aataaggcga aaaatataaa gccgggaaga aaggcatctc
       61 gctttatgaa ataaaacatg gcaacgattc cgatgattgg agcgagacag ccaaaccgta
      121 cataccaatt ggattgtgtg gaagacaaaa gaacccctca tttcaaaaat gatagaaact
      181 agcaactcga atcgatttgt atttatcata tcgaaaggaa aagcgaataa caatgtcaaa
      241 ataaaaagct gcccccacgt caacggggac agcaattcca aaatcagttc acttgtgctg
      301 cttcaatctc ttcagtcaat tcaacgagct gatcgatcaa ttcgccgata taggcaatgg
      361 tatcaagcaa aggctgttcc gtcgtaatgt cgacgcccgc cattttggaa agctctgcag
      421 gagactttgt accgcccgct tgaagcactt tcagccagtc ctcgacagca ggttgccctt
      481 cctccaaaat acgcttcgat acttgagtgg aaattgtcaa tccggcgctg tatgtgtatg
      541 gatacaagcc catgtagtaa tgcggctggc gcatccatgt caattctgca ccttcgttga
      601 ttttcacatc gtctccccag aattcttcaa ggacgccgcg cttcaggctg ttcagtacat
      661 tcgcattcac actgccgccc gcatcaatcc gctcgtacac ttttcgctgg tatgcagctt
      721 ccaataggtg agtgacgaag ttatggtagt acgtacgtgc aacaatggaa gaaatgaccc
      781 agcgcttgaa tttcggatca tccgaattgg aaagcaagtg gttcgccatc agcatttcat
      841 tcatcgtcga aggagcttcg atgaagtaca tggaagggcg tgaattgaaa atgttctggt
      901 gttggtgagc attgtagaaa tgccccgcat ggccaagctc atgcgccaat acaaagacat
      961 cttccatgct gcctgtccag gaaatcaaaa tatatggatg gacgccataa gggctagaac
     1021 agaaagcgcc tgtcgatttg cccgcgttct gtgcaaaatc tgtccaacgc tctttatagg
     1081 agcggtcgat catgttcatg taatcgtcgc ccatgatggc aagtgcgtca ttaatatatt
     1141 tctttgattc ttcaattgtg atcttcggct catagttcgg atccaacgag atcttcaaat
     1201 ccgagaaagt catctcgtcc aggccgtgaa ccttttgaag aagctttgca tatttgcgca
     1261 tatgcggcgc aagatgttcc atgatcaaat cgatttggcg gtcgtacatg gagcggtcga
     1321 cttcctggtt gaaaaggagg taatcaaaga cattatcata tcctctaaga tcggaagttg
     1381 ttttctcctt ctgcaagtgc atatcgtacg tcttcgcagt cgtatgctga tactcccgca
     1441 attttgcatg gaaagcatca tacgcagctc ttctcttttt cgtatctgtt tccgactccc
     1501 atccgccttc gaatgtcgta tagcttaatg ggtatttttc gccgtcgact tcaaaatcat
     1561 caaagttcat gtcgactaat tttgtcgtat tgtatagccc gtatggcgcg ttcaatacgg
     1621 aagagaatgc cgccaatgtc ttttcgactt caggatgaag cttatacggt ttttgacgga
     1681 tcaattcttc aaggtagttt ttatactcct cggattggtt catcgcgttt tccaaaagct
     1741 cactcggcac ttccgaaagt tcgcttgtga agaatgacag ctgacttcca agtttggaag
     1801 cgatcgagcc gtatttgctt gcgcgcattt gtgcttcgtc attcgtttgg tcgacgctat
     1861 gcgaaagact cgtgtacgtt ccgatcggca ccattttgcc catgatggaa gcatatccat
     1921 ccaaagcttc aagaattact ttctcgtcgg tgattttccc cttgaagcgc tcttcgaagg
     1981 aagcgacttc actttcaagc tgctcaattg ctgcattata ctcctcttca gttttgaaaa
     2041 ggtcttccaa attccatgtc tcttctacgt tcacggctga acgcggtggt agacttttca
     2101 ccattaacca tctctcccta ttcgttcaat ttattacgga tgtcgaacta atgatagtat
     2161 atagcaaact tatagaaaag taaaaatgaa ttggtatttc tgtaatctta ttttttactt
     2221 tcttttcaac cgtccgattt catctgtgac tacaaaagct agatcttcat tgccaaaaag
     2281 ttgaaggaca tcgagcatcg tttcgttatc aatatggaga tcgtcgaatc catcatcact
     2341 ttcgatgact tcctgtaaag ctgcgttttc catttggtaa ggatatgcct ttttatagag
     2401 cgcatgagga tccaattcat tgttcaagca ccattgcacg aaaaggcgga tcatcagcgt
     2461 ttcatcctcc ctatacttct gaattatttg ttcatcgata tttctgaatt ccattgtcca
     2521 tccctccggt gataagctat gattctaagt atatatgaaa tgggggattt cgatcatgaa
     2581 tcgtgtcgat gttgtttatt cagctatcct aaaaaaggga aaagtactga tggtgaagaa
     2641 taagaaatat gataattgga cattgccggg cggtggtgtg gaaaaagggg agacattgga
     2701 acaggctgca gttcgtgaag tttgggaaga aaccggctta acagtaaaaa tcgggagctt
     2761 gctaacggtg aatgaagcgt tcaggcaaga ggaaaataac catgtgctat ttttcacgtt
     2821 ttctgcggaa gtggtggaag gggaagtggc catcaaggac acaggcggca ttttagaagc
     2881 gggatggaaa gatctttcat tcgctgatta agccatgcct tattacgaag gcggttttga
     2941 aaagctcctg gaaatgtcga taccatatac atttcaggga gttcaggctt gaacagggaa
//
LOCUS       DASULI010000390         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1143262,
            whole genome shotgun sequence.
ACCESSION   DASULI010000390 DASULI010000000
VERSION     DASULI010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143262"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFX34_08430"
     CDS             complement(<1..729)
                     /locus_tag="VFX34_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5564972.1"
                     /translation="MKRISEWGRKFSSFNRNVRFFIIGNALIQIGMGVFMVMYNLYIR
                     ELGMPETVNGKVISMTAMASAIMLVPAGFLSDRFGRKWMIVGGAVLTVTTLFYRGIAV
                     AETPIITAAFLTGLFMAFVQVSGIPFLAENSSPTERVKLFSVNFAIITIANVIGSLAG
                     GIIADVLETVFRLSAIEAIRYALLLGAAIFTIGLIPLFQLKDKPTETAKVKKEYTEEA
                     SAADDSFKRNLIVIFHFSFASLLIG"
     gene            complement(1350..>2604)
                     /locus_tag="VFX34_08435"
     CDS             complement(1350..>2604)
                     /locus_tag="VFX34_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5564973.1"
                     /translation="TLFVLPVLYIWMKLYRRFAAKVNHIIRSKNSEMNAMINESINGM
                     TIIQAFRREKQMDGEFNELNDEHYKYQSKLLKVEAATSHNLVDIIRSLAFVFLIWYFG
                     GASLSAESVISVGMLYAFVDYITRLFNPITGVVNQFARLEHSLVAAERVFGLMDRQGE
                     PVSDEKIARYRGNVRFENVWFAYKDEEYVLKDISFEAKQGETVALVGHTGSGKSSIMN
                     LLFRFYDVSKGKITIDGMDIGNMSRQTIRDHMGIVLQDPYLFSGTVESNISLGDARIS
                     REKVQEALDAVGGDRVLKHLPGGIDEPVVEKGSTLSSGQRQLISFARALAFDPAILIL
                     DEATSNIDTETEEIIQHAMDVLKKGRTTFIIAHRLSTIKNADRILVLDRGEIVEQGNH
                     DELLALGGQYAQMYKLQAGNGSKVG"
BASE COUNT          666 a          698 c          557 g          683 t
ORIGIN      
        1 accgatcagc aggcttgcga atgaaaaatg gaagatgacg atgagattcc gtttaaagct
       61 gtcatccgcg gccgaagctt cctcagtgta ttccttcttc acttttgctg tctcggtcgg
      121 cttatcctta agctggaata aaggaatcaa accgattgta aaaatagccg cgcctaatag
      181 caatgcatag cgtatcgctt caattgcgct tagccggaac acagtttcca aaacatccgc
      241 gatgattccg cctgccaaac ttccgatgac attcgcgatt gtgatgattg cgaagttgac
      301 gctgaacagc ttgacccgtt ctgtcggtga ggaattctcc gcaagaaacg ggatgcccga
      361 tacttgcacg aatgccatga ataaacccgt caaaaatgcg gcagtaataa tcggcgtctc
      421 cgcaaccgcg attccccggt agaataatgt cgtcactgtt aaaacggcac cacccacaat
      481 catccattta cggccgaatc gatcgcttaa aaagcctgca ggaacgagca tgatcgcgga
      541 agccattgcg gtcatggaga tgacttttcc gtttactgtt tcaggcatgc cgagctctcg
      601 tatgtataaa ttgtacatga ccataaaaac acccatcccg atttggatga gcgcattccc
      661 tatgataaaa aacctgacat tacgattgaa ggaggaaaat ttacgtcccc attcactgat
      721 ccttttcatc cgcatttcca actttccgcg acaaatactt cgatactcta aatatacgtg
      781 tttacgaaac atttcgcaac ggaattttca tttttgtcag tacattcgtt gcgctatatg
      841 cctcgtctat attcacttac agtctttctg aaaggatccg gattttccac ataacttccg
      901 atgacccatc cgcaatccgt gcaaatcaaa tgtatcaaac tatggttaaa tcccattctt
      961 tcgtaaggcg ttacaccccc ataacctcct tgcttacctc tgcctatctc gtggctgccg
     1021 catttcggac attcctccac tttgaaactc accactataa tcgcctcatt tcttctgaat
     1081 gctagaatag taccttattt catttacggt tcgacgacat gaaagtttca gcaaatttgg
     1141 cagattcagg acggcttgct ttgagcgcgg aatggatcgc tttgagcgca gatcggcttg
     1201 ctttgagcgc gagacggctc gctttgagcg ggggacgaat tgctttgagc gtggaaaggc
     1261 tcgctttgga cgcgcgccat ccccacaaca aaaaaactgc cacagaaagt cgtcttctcg
     1321 actttccatg acagcctcta cctattaatt taccccactt tagaaccgtt ccccgcctgc
     1381 aatttataca tctgagcata ttggccgccg agcgcgagca attcatcgtg attgccttgc
     1441 tcgacgattt caccgcggtc caacactaaa atgcggtctg catttttgat cgtggacaaa
     1501 cggtgggcga tgatgaacgt cgtccgtccc ttcttcaaca catccatcgc atgctggata
     1561 atttcctcgg tttccgtatc gatgttcgaa gtcgcttcat ccagaataag aatcgccggg
     1621 tcaaaggcta gcgcgcgtgc gaatgaaatg agctggcgct ggccggatga aagcgtgctg
     1681 cctttctcca cgaccggctc gtcaatcccg ccaggcaaat gcttcaacac ccggtcgcca
     1741 ccgaccgcgt caagcgcctc ttggactttt tcacgggaaa tgcgtgcatc acccaagctg
     1801 atgttggatt ctaccgttcc gctgaataaa tacggatcct gcaaaacgat tcccatatga
     1861 tcgcggatcg tctggcgtga catgttcccg atatccattc catcaatcgt gattttccct
     1921 ttcgaaacat catagaaacg gaataacagg ttcatgatgg agcttttccc ggaacctgta
     1981 tggccgacaa gcgcgacggt ctccccttgc ttcgcctcga aggaaatgtc cttcagtaca
     2041 tattcctcat ctttataagc gaaccagaca ttttcgaatc tgacgttccc ccgataacgg
     2101 gcaatctttt catcgctgac cggctcgcct tggcgatcca taagaccgaa gacgcgttcc
     2161 gcagcgacaa gtgaatgctc aagccgcgcg aactggttga cgactcccgt aatcgggttg
     2221 aacaaccgcg taatataatc gacgaatgcg tatagcatac cgacagaaat gacgctctcc
     2281 gccgacagcg atgcaccgcc gaaataccag atcagaaaga cgaaagccaa tgagcggata
     2341 atgtcgacaa ggttatggga tgtcgccgct tcgactttca ataatttgct ttgatactta
     2401 taatgctcat cattcaattc gttgaattcc ccatccatct gcttttcccg ccggaaagcc
     2461 tggatgatcg tcatgccgtt gatcgattca ttgatcatcg cattcatctc actgtttttg
     2521 ctgcgaatga tatggttcac ctttgctgcg aatcgtctgt acagcttcat ccaaatataa
     2581 aggaccggca agacgaaaag cgtg
//
LOCUS       DASULI010000391         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_89875, whole
            genome shotgun sequence.
ACCESSION   DASULI010000391 DASULI010000000
VERSION     DASULI010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89875"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /gene="hemW"
                     /locus_tag="VFX34_08440"
     CDS             complement(<1..638)
                     /gene="hemW"
                     /locus_tag="VFX34_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5564974.1"
                     /translation="MRGMYIHIPFCHQICFYCDFNKVFFKNQPVDEYIESIGNELAIM
                     ANEGHSFDGLETVFLGGGTPTSLSEKQLDRLLEIIHRHVDVRSLREFSTEANPDELTR
                     GKLSVLKNGGVGRLSIGVQSFDEQLLKRIGRTHGADTPEKIIRDAREIGFENISIDLI
                     YGLPEQSIRQWEDTLDKALSLELPHYSGYSLIVEPKTVFYNLMNKGKLPLPG"
     gene            complement(830..>2128)
                     /gene="lepA"
                     /locus_tag="VFX34_08445"
     CDS             complement(830..>2128)
                     /gene="lepA"
                     /locus_tag="VFX34_08445"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019382868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX5564975.1"
                     /translation="IGIEEILEQIVEKVPAPVGDPEAPLKALIFDSHYDQYKGVIVNI
                     RIVEGTVKPGDKIRMMATGKEFEVIETGVFTPNISMRDELSVGDVGFLSAAIKNVGDT
                     RVGDTITNAKNPASEPLPGYRRLNPMVFCGLYPIDSSKYVDLREALEKLELNDSALEY
                     EAETSQALGFGYRCGFLGLLHMEIIQERIEREFKIDLITTAPSVIYNVVTTDGKELKV
                     DNPSMMPDAQKIDHVEEPYVKASIMVPNEYVGSVMELCQQKRGNFITMDYLDASRVNI
                     IYELPLAEIVYDFFDQLKSGTKGYASFDYELIGYKTSKLVKMDILLNGEQVDALSFIV
                     HRDFSYERGKAIVEKLKNLIPRQQFEVPVQAAIGQKIVARSTIKSIGKNVLAKCYGGD
                     ISRKRKLLEKQKEGKKRMKQVGSVEVPQEAFMAVLKMDED"
BASE COUNT          505 a          525 c          472 g          626 t
ORIGIN      
        1 tctcctggca aaggcagctt ccctttattc atcaagttat aaaagacggt ttttggctcg
       61 acgatcaggg agtatccgga ataatggggc agttccaatg acagtgcttt atcaagcgta
      121 tcctcccatt gtcggatgga ctgttccggc aatccataga tgagatcgat gctgatgttt
      181 tcaaagccta tttcacgcgc gtcacggata attttttccg gagtatccgc accatgcgtc
      241 ctacctatcc ttttcaatag ttgctcgtcg aaagactgga cgccgatgct taaccggcca
      301 acgccgccgt ttttaaggac ggataatttt ccgcgggtca gctcatccgg attcgcttcc
      361 gtcgaaaatt ccctcaaaga acggacatcg acatgccgat ggatgatttc aagtaatcgg
      421 tccaattgtt tttctgataa tgaagttggt gttcctcctc cgaggaaaac ggtttcgagc
      481 ccgtcgaatg aatggccttc attcgccatg atggcaagtt cgtttccgat tgattcgata
      541 tactcatcta ccggctggtt tttaaagaac actttattga aatcgcagta gaagcaaatc
      601 tggtggcaga aaggaatatg aatatacatg cctcgcattt ttgttgtgtc ccctttcttg
      661 tcagtgcaaa aaggagcccg aaacggactc cttttaattt tagatatcaa tcttcgtcca
      721 gctacgggcg ccagagactc ggggtcataa gtcaaagatg ctctgtggca aaaagcgcca
      781 ctccgcttct tcgccttatg cctgtcgtct ctagcaggcg cccttcgctt tagtcttcat
      841 ccattttaag aacagccatg aacgcttctt gtgggacttc cacggagccg acttgcttca
      901 tccgcttttt cccttctttt tgcttctcga gcaatttacg tttacgggag atatctccac
      961 cgtaacattt tgcaagaacg tttttaccga tcgatttgat tgtcgagcgg gcaacaatct
     1021 tttgaccgat tgcagcttgg acaggcactt cgaactgctg acgcgggatg aggttcttca
     1081 atttttcaac aatcgccttg ccccgttcgt agctgaagtc gcggtggacg atgaagctca
     1141 gcgcgtcgac ttgttcacca ttcaataaga tgtccatttt gacaagtttc gatgttttat
     1201 agccgatcag ttcataatcg aatgacgcgt aacctttcgt ccccgatttt aattggtcga
     1261 aaaagtcata gacgatttct gcaagaggca gttcatatat gatattgaca cgtgaagcat
     1321 ccaagtaatc cattgtgatg aagttgccgc gtttttgctg gcataattcc atgacggatc
     1381 cgacgtattc gttcggcacc atgatcgatg ctttcacata aggctcttcg acatggtcga
     1441 ttttttgtgc atccggcatc atggaagggt tatcgacttt caattctttc ccgtccgtcg
     1501 tgacgacatt gtaaataacg cttggtgccg tggtgatcaa gtcgatcttg aattcacgtt
     1561 caatccgttc ctggatgatt tccatatgga gcaatccaag gaatccgcaa cggtacccga
     1621 agcccaatgc ctgggaagtt tccgcttcat attccaaagc ggagtcgttc agctcaagtt
     1681 tttcaagcgc ttcacgcaaa tcgacatatt tggatgagtc aatcgggtaa agcccgcaaa
     1741 acaccatggg attcaatcga cgataccctg gcaacggttc actagccggg tttttcgcat
     1801 tcgtgatcgt gtccccgact cgcgtatccc cgacattttt aatggccgcg gaaaggaaac
     1861 ctacatcccc aaccgatagt tcatcgcgca tggatatgtt cggtgtgaac actcctgtct
     1921 cgatgacttc gaattctttg ccggttgcca tcatgcggat tttatcacca ggctttacag
     1981 taccttcaac aattcggata ttgacgatga cgcctttgta ttggtcataa tgcgagtcga
     2041 aaatcaatgc tttcaacggc gcttccggat ctcctacagg tgcaggaact ttctctacaa
     2101 tctgctcgag gatctcttct attccgat
//
LOCUS       DASULI010000392         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1062642,
            whole genome shotgun sequence.
ACCESSION   DASULI010000392 DASULI010000000
VERSION     DASULI010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062642"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFX34_08450"
     CDS             <1..211
                     /locus_tag="VFX34_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564976.1"
                     /translation="KEIGLESFNAALFIPVNEHAAEQVHFRLIPRMTIGALETSDMNV
                     FNYLHGEPLSLKVPEEVARNAAKFF"
     gene            233..1012
                     /locus_tag="VFX34_08455"
     CDS             233..1012
                     /locus_tag="VFX34_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX5564977.1"
                     /translation="MNKTVVDRVGRTVTYPYPPKRIVSLCPGITDTLFALDLEAEIVG
                     RTRFCIFPKGKVELVPAVAGTKDIKLEAIQDVRPDLIIVEKEENTKEIVEELEKLFPV
                     YVAEVQSVDDAFRMIEDMGSLTDRTEAAARLIANIHQQFESLPKAIGKRIAYVIWRKP
                     YMVVGKDTYINSLLEQMGFINPFTEADGRYPAVTADEFQEAELDYVLLASEPFPFKEK
                     HFKEFLHMMPDTEPILVDGEMFWYGPRMLEAAHYFQRNLTI"
     gene            complement(1055..1462)
                     /locus_tag="VFX34_08460"
     CDS             complement(1055..1462)
                     /locus_tag="VFX34_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5564978.1"
                     /translation="MRKGADVFVDKLKEAIEGEYKDYYFYKSMYEKTDDPLWKDFIQH
                     AYEDEKSHYEMFQQLYYMMTGTFVQNPKKPIPCYNFKQCVKQALVGELDGVEMYKEML
                     LTIPFQQAYNPLFIAMHDEMEHAIRMSTIYNSL"
     gene            1516..1719
                     /locus_tag="VFX34_08465"
     CDS             1516..1719
                     /locus_tag="VFX34_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheE family protein"
                     /protein_id="HEX5564979.1"
                     /translation="MLQHFSYKPMFAGGSLPGWTFSFFYQNERFTGDYKPDGTIVWTS
                     GTPADEENVKKMIHELMTFHVYE"
     gene            1767..1919
                     /locus_tag="VFX34_08470"
     CDS             1767..1919
                     /locus_tag="VFX34_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5564980.1"
                     /translation="MDKEKTPEQLQKEKEQLKVQNQKTENVFEDKSRVPDEQNAWKET
                     QYRRGE"
     gene            complement(2087..>2617)
                     /locus_tag="VFX34_08475"
     CDS             complement(2087..>2617)
                     /locus_tag="VFX34_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5564981.1"
                     /translation="FGSGLVIPYLNLYFANRFDASNSYIGLVLSLGSAMTAVAAMIGP
                     ALSRKVGKVKAIILFQVLSIPFLLLTAYTTSLFLASLGFLLRQALMNAGNPIQSAVAM
                     EVVSDKYKGLANSMNQMVFNLGWATMGSVATGLVVANGSYWGYAYAFTITAGLYIISS
                     VYYYFIFGRRKLAGEK"
BASE COUNT          767 a          589 c          607 g          654 t
ORIGIN      
        1 caaggaaatc ggattggaaa gcttcaatgc tgcattgttc atccctgtga acgagcatgc
       61 ggctgagcaa gtccatttcc ggctgattcc ccgcatgacg atcggggcgt tggagacgag
      121 cgatatgaac gtgttcaatt atttgcatgg tgagccttta tcattgaaag ttcctgaaga
      181 agttgcccgt aatgcagcta agtttttctg aagaaaggat tgtggcttag ttttgaataa
      241 aacagttgtc gatcgggtcg gaagaactgt cacgtatccc tatccaccaa aacggatcgt
      301 ttcgttatgt cccggcatta ccgatacatt atttgcgctc gacctggagg cggaaattgt
      361 cggccggacc cggttttgca ttttcccgaa aggcaaggtg gaacttgtac cggctgttgc
      421 aggcacaaag gatattaaac tggaagcgat ccaagacgtt cgaccggacc ttatcatcgt
      481 tgaaaaagag gaaaacacga aagagatcgt ggaggaactg gagaagctct tccccgtcta
      541 tgtcgccgaa gtccaatccg tggatgacgc atttcggatg atcgaggata tgggaagcct
      601 gacggatcgg accgaagccg cagcaaggtt aattgccaac atccatcaac aattcgaatc
      661 attgccgaaa gctatcggga aacggattgc ctatgtcata tggcggaaac catatatggt
      721 cgtcgggaaa gatacataca tcaattcgtt attggaacag atgggattca tcaatccgtt
      781 caccgaagcg gatggcaggt atcccgcagt tactgctgac gaatttcaag aagccgaact
      841 ggattatgta ttgctggcat cagagccttt cccgttcaaa gaaaagcatt tcaaagagtt
      901 tttgcacatg atgccggaca ctgaacccat cctggtcgat ggggaaatgt tctggtacgg
      961 tccaaggatg ttagaagctg cacactattt ccaacggaat ttgaccatat aaaaaaacac
     1021 gggatcatcg taaagatgtt ctcgtgtttt ttattcataa tgaattatag atcgtcgaca
     1081 tccggatggc atgctccatt tcatcatgca tggcgatgaa taaaggattg taagcttgtt
     1141 ggaaaggaat cgtcaacagc atttccttgt acatttccac cccatccaat tcaccgacga
     1201 gcgcctgttt aacgcattgt ttgaaattat agcacggaat cggcttcttt ggattttgta
     1261 caaacgtccc ggtcatcata taatagagtt gttgaaacat ttcataatgg cttttctcat
     1321 cctcatacgc atgctggatg aagtccttcc aaagcggatc gtccgttttc tcatacatcg
     1381 acttgtaaaa ataatagtct ttgtattcac cctcgattgc ctcttttaat ttatcgacaa
     1441 acacgtcagc accctttcgc accttttgta cactttatgc gagcgggcgc ttgtctatga
     1501 aaggagcgat tcaatatgct tcagcatttc agctataaac cgatgtttgc gggcggcagc
     1561 ttgcccggtt ggacgttttc atttttttat caaaatgaac gtttcactgg agactacaag
     1621 ccggacggaa caatcgtctg gactagcgga acaccagcag acgaagaaaa tgtcaaaaaa
     1681 atgatccatg agctgatgac attccacgtt tatgaatgat ggaatcattt ccgtacatac
     1741 tatccctcga taaggaggcg atggatgtgg ataaggaaaa aacgccagaa caattgcaaa
     1801 aggaaaaaga acagttgaaa gtgcaaaacc agaaaactga aaacgtcttt gaagataaaa
     1861 gccgggtacc tgacgagcag aatgcttgga aggaaaccca gtatcgaaga ggggaataac
     1921 caaaagcgaa gaacgcctgc ttagaggcga caggcataag gcgaagatgc ggcgtggcgc
     1981 tttttgccac agagcatctt tgacttatga ccccgagcct ctggcgcacg aagtctagac
     2041 gataacaaaa gcgaagaacg ccaaagttta gaggcgccct tcgcttttat ttttcgccgg
     2101 ccaacttcct tctgccgaaa atgaaataat aataaactga tgaaataata tacagtcccg
     2161 cagtaatcgt gaatgcatat gcataccccc aataggatcc gtttgcaacg accaatcctg
     2221 tcgctacact tcccatggtt gcccatccta agttgaatac catctgattc atggaattgg
     2281 cgagcccttt gtatttgtcg gacacgactt ccattgccac tgcactttgg atcgggttgc
     2341 ccgcattcat aagcgcctgg cggaggagaa agccaagcga cgccaagaat aaagatgtcg
     2401 tatacgccgt cagcaatagg aacggaatcg acaacacttg gaataaaatg atcgccttca
     2461 ccttgccgac tttccgcgac agtgctggac cgatcatcgc tgcgactgcg gtcatcgccg
     2521 aacctaatga taatacaagc ccgatatagg aattcgatgc atcaaaacgg ttcgcgaaat
     2581 acaaattcaa atacggaatg actaaaccag atccaaa
//
LOCUS       DASULI010000393         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_596102, whole
            genome shotgun sequence.
ACCESSION   DASULI010000393 DASULI010000000
VERSION     DASULI010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596102"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /gene="smpB"
                     /locus_tag="VFX34_08480"
     CDS             complement(<1..412)
                     /gene="smpB"
                     /locus_tag="VFX34_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5564982.1"
                     /translation="MPKGMGKTVAQNKKANHDYFIEETFEAGIVLQGTEIKSVRNGKV
                     QLRDAFVRIRNNEAWISNMHISPYEQGNRFNHDPIRSRKLLLHKRQINQLIGTIKEQG
                     ITIVPIKMYMKDGYAKVLIGIGKGKKNYDKREDLK"
     gene            complement(493..>2154)
                     /gene="rnr"
                     /locus_tag="VFX34_08485"
     CDS             complement(493..>2154)
                     /gene="rnr"
                     /locus_tag="VFX34_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX5564983.1"
                     /translation="IETDFPQEVIDQANKVPDEITEKDLFKRRDLRDEVIVTIDGADA
                     KDLDDAVTVTKNPDGTYKLGVHIADVSYYVTENSPLDQEAFDRATSIYLTDRVIPMIP
                     HRLSNGICSLNPQVDRLTLSCEMIIDGSGKVVSHEIFQSVIKTTERMTYTDVYKILEE
                     KDEELIKRYEPLVPMFESMAELAAILRKKRMERGAIDFDFKESKVIVDEKGWPTDVVI
                     RERTVSEKLIEEFMLAANETVAEHFHWMQVPFIYRIHEDPKPEKLQRFFEFLTNFGLV
                     VKGTGNQIHPRALQEIVEAIEGTPEETVISTMLLRSMQQAKYFEESLGHFGLAADFYT
                     HFTSPIRRYPDLIVHRLIRTYLIEGDVSSSTVGHWGARMPEIAQHTSERERRAVDAER
                     DTESLKKAQYMLDKIGEEFEGIISSVTNFGMFVELENTIEGLVHVSYLVDDYYHFDDR
                     QMMMIGQHSGKQYRIGDEVTVRVVAVKPEESAIDFELVGMKKTFHRARPDAPKVIRAK
                     KKDGAPGQRGQKDQKKDDKNKPKNNKKKKFYENVPKSKSRKRKNK"
BASE COUNT          517 a          536 c          423 g          678 t
ORIGIN      
        1 tcttaaggtc ttcacgttta tcgtagttct tcttcccttt accgattccg atcaggactt
       61 ttgcgtaacc atccttcatg tacatcttga tcggtacaat tgttatcccc tgctccttga
      121 tcgttccaat gagctggtta atttggcgtt tatggagcaa tagtttcctt gaacgtatcg
      181 gatcgtggtt gaagcgattt ccttgttcat atggactaat atgcatattc gaaatccaag
      241 cttcattgtt ccggatccgg acaaaagcat cccttagttg gacttttcca ttccggacag
      301 atttgatttc tgtcccttgc aggacgattc cggcttcaaa tgtttcttct ataaagtaat
      361 catggttcgc cttcttgttt tgggcgaccg ttttccccat tccttttggc attcttctca
      421 ccctctaacg cagcaacttg tatcttacat tgtattaaat aaatgaaggg gacggcaagt
      481 gccgccccac tattatttgt tcttacgttt tctgctcttt gatttcggaa cattttcgta
      541 gaactttttc ttcttgttgt ttttcggttt attcttatcg tctttctttt gatctttctg
      601 gccacgttga ccaggtgcgc cgtccttttt cttcgcacgg ataaccttcg gtgcatctgg
      661 acgtgcacga tggaatgttt tcttcatgcc gacgagttca aaatcaatgg cagattcctc
      721 tggtttgact gcaacgacac gtacggttac ttcatcacca atacggtatt gcttaccgct
      781 atgctgacct atcatcatca tttgtcgatc atcgaagtgg taataatcat ccactaaata
      841 gctcacatgg acaagaccct cgatcgtgtt ttcaagctca acgaacatac cgaagttcgt
      901 aacggaacta atgatgccct cgaactcttc accaatcttg tcgagcatgt actgagcttt
      961 cttcaatgat tcagtatccc gttcagcatc aaccgcacgt ctttcacgct cagatgtatg
     1021 ctgggcgatt tccggcattc gagctcccca atgtcctaca gttgagcttg atacatcacc
     1081 ttcgattaaa taagtgcgga tcaatcggtg gacgatcaaa tccggatatc gacgaatcgg
     1141 cgatgtaaag tgagtataga aatcagcggc aagcccaaag tggcctaagc tttcttcgaa
     1201 atacttcgcc tgctgcatcg aacgtaatag catggtcgaa ataactgttt cttccggagt
     1261 cccctcgatc gcctcaacaa tttcctgaag ggctctcgga tggatttggt tgcccgttcc
     1321 tttcacgaca agtccgaagt tcgtcaggaa ttcaaagaaa cgttgtagtt tttccggttt
     1381 cggatcttcg tggatccggt aaatgaacgg aacttgcatc caatggaagt gttctgctac
     1441 cgtttcgttg gctgccaaca tgaattcctc aatcagcttt tcagaaacgg tacgctctcg
     1501 aatgacaaca tccgtcggcc agcccttttc atctacgatt actttcgatt ccttgaaatc
     1561 gaagtcaatc gcacctcgct ccatccgctt tttacgcaag atagctgcaa gctcggccat
     1621 cgattcaaac attggcacta aaggctcata acgtttgatg agttcttcgt ccttctcttc
     1681 caaaatctta taaacatccg tataagtcat tcgctcagtc gtcttaatga cactttggaa
     1741 aatctcgtga gaaacgactt taccgcttcc atcaatgatc atttcacaag acaacgtcaa
     1801 acggtccacc tgcggattaa gtgaacagat gccattggaa agtcgatgtg gaatcatcgg
     1861 aattacacgg tctgtcagat agatactcgt tgcacggtca aatgcttcct ggtcaagagg
     1921 agaattctct gtcacatagt agctgacatc cgcgatatgg acacccaatt tatacgttcc
     1981 atccggattt ttcgtaaccg taacggcatc gtccaagtct ttcgcgtctg caccatcgat
     2041 tgtgacaatc acttcatcgc gcaaatcacg acgtttgaac aaatcctttt cggtaatctc
     2101 gtccggcacc ttattggcct gatcaattac ttcttgtggg aaatcagttt caat
//
LOCUS       DASULI010000394         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_778076, whole
            genome shotgun sequence.
ACCESSION   DASULI010000394 DASULI010000000
VERSION     DASULI010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778076"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1668
                     /locus_tag="VFX34_08490"
     CDS             121..1668
                     /locus_tag="VFX34_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016765995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5564984.1"
                     /translation="MPVEQEKKTVLPLTGKEYLESLKDGREIWLHGEKVKDVTTHPAF
                     RNAARSIARLYDALHDEKTKDVLTVETDTWNGGFTQKYFRVDKSSQDLLESRDAIAQW
                     ARLTYGQMGRSPDYKAAFLATLGADPDYYGKYADNARRWYKEAQERNWFFNHAIINPP
                     VDRHKPLDAVADIFVRAVGETEQGVIVSGAKMVATGSALTNYNFVAHYGAAPITKEEF
                     ALVFVAAMDTPGVKLVSRASYEMNAAVMGSPFDYPLSSRFDENDSVLIFDNALIPWEN
                     IIIYKDVEKANSFFADSGFLHRFTFHGVTRLAVKLDFVSGLLLKAVKMNGSDQFRGVQ
                     VNVGEVLAWKNMFWALSDAMALNPDEGRNGTKLPNLNYGLAYRMFMSEGWPRVKEIIE
                     NVVAGSLIAQPSSSRDFQNPELRPLLDKLYRGSYGVGAEEKIKLIKLLWDAIGTEYGG
                     RHELYERNYSGNHENIRLENLVVANMSGQADQFEKFAEECLNDYDLNGWTNDTWINPD
                     DVSYFKE"
     gene            1665..2516
                     /locus_tag="VFX34_08495"
     CDS             1665..2516
                     /locus_tag="VFX34_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5564985.1"
                     /translation="MIKRIYKVELEGRYLQIADYPGEKGTIIALHGLTGTHKNMLHYA
                     QSLQGDYRFIACDLRGRGDSSGMDDVPSIFSHAEDVEELIAVLGIKQPILLGHSMGAF
                     ISAIVASELPSVKGLVLLDGAARMSGRQHEIVRPSLGRLRKTYESKRAYVEELKQLYK
                     RLGIKWTDAVQEIVEYEIHGTGNGWMHKSANENIEEDFASFDFFNPIKVMGKITCKTL
                     LIQADGGIGTFPPFFTDADYEETKKYTSKLTTVNSDSNHYTMVFEKQEAILDEIRKFL
                     DGVILDE"
     gene            2509..3216
                     /locus_tag="VFX34_08500"
     CDS             2509..3216
                     /locus_tag="VFX34_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5564986.1"
                     /translation="MSEGLAYFKEVYGTVPGWVGKMHDYRPDALDHYTSLRSAIMEDG
                     LLSRKEKDLLLVGINAARCYERSMVYHTKGAIDGGATIPELIEYLLVAYRYGGLQSMQ
                     TGIQSVKYALALNGLPEGKVPYGICNEHILSYFISVVDEEDAWFVSNLLKAPEELFND
                     LLLVRGLVSAKMKFLLMVGIHSTALQGTEAGKWMEKARGSGATEGELAEVGLICLLTA
                     GIPAWFEASDSLREGVG"
     gene            3218..>3456
                     /locus_tag="VFX34_08505"
     CDS             3218..>3456
                     /locus_tag="VFX34_08505"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5564987.1"
                     /translation="MAIVYERVENALSAIKSGMTIMVGGFGLVGAPLTLIEGLTEKDI
                     EGLTIISNNLGEAGEGLGMLLRQQKIRKAIGSYFT"
BASE COUNT          916 a          760 c         1023 g          757 t
ORIGIN      
        1 aggaatcgtt caccggaaag ttagccattt ttggtggaac gagtcttttt attcacttcg
       61 gcatttttcc taggagacag gcatacgtat aggtgtaatg ggacaaggag gagtgaattc
      121 atgccagttg aacaggaaaa gaagacggta ttgcctttga caggcaagga gtatttggaa
      181 agcttgaagg acggacggga aatttggctg catggcgaga aggtgaagga tgtgacgacg
      241 catcccgcat tccggaatgc tgcgcgctcg atcgcccgcc tgtacgatgc gctgcatgac
      301 gaaaagacga aggatgttct gacggtcgag acggatacgt ggaatggcgg tttcacacag
      361 aaatattttc gtgtggacaa gagttcgcaa gacttattgg agtcgcgcga cgcgattgcg
      421 caatgggctc ggctgacgta cggccagatg ggaagatcgc ccgattataa ggccgcattc
      481 ctcgcaacgc tcggtgcgga ccctgactac tatgggaaat acgcggataa tgcgcgcaga
      541 tggtacaaag aggcgcagga gcggaattgg ttcttcaacc atgcgatcat caatccgccg
      601 gtggacaggc ataagccgct cgatgcggtt gccgacattt tcgtccgggc tgtcggcgag
      661 accgagcaag gcgtcatcgt cagtggagcg aagatggtcg cgacgggatc cgcattgaca
      721 aactataatt tcgtcgccca ttacggcgct gcgccgatca cgaaagagga gttcgcactc
      781 gtctttgtag cggcgatgga tacgccgggc gtgaagcttg tgagccgtgc ttcatatgaa
      841 atgaacgccg ctgtcatggg aagtccgttc gactatccat tgagcagccg attcgatgaa
      901 aacgactcgg tcctcatttt cgacaacgcg ctcattccat gggagaacat catcatttac
      961 aaagatgtgg agaaggcaaa cagctttttc gcagacagcg gattcctgca cagattcaca
     1021 ttccacggcg tgacaagact tgcggtaaaa ctcgacttcg tttcaggttt attgctgaaa
     1081 gccgtcaaga tgaacggttc cgaccaattc cgcggagtcc aagtgaacgt cggcgaggtg
     1141 cttgcatgga agaacatgtt ctgggcactg agcgacgcga tggcgttgaa cccggacgaa
     1201 gggcggaatg ggacaaagct gccgaatttg aattacgggc tcgcataccg catgttcatg
     1261 tccgaagggt ggccgcgggt gaaggaaatc atagaaaacg tcgtcgcggg aagtctgatc
     1321 gcacagccgt ccagctcaag agacttccaa aatccggagc tgcgtccgct gttggataaa
     1381 ttataccgcg gttcatacgg cgtaggggcc gaggagaaaa tcaagctcat caaattgctg
     1441 tgggatgcaa tcggaacgga atatggcggc aggcatgaac tgtatgagcg caactattcc
     1501 ggcaaccacg agaacatccg gcttgaaaac ttggtcgtcg ccaatatgag cgggcaagcg
     1561 gatcagttcg agaaattcgc agaggaatgc ttgaatgact acgatttgaa cggctggaca
     1621 aatgacacat ggattaatcc ggacgatgtg agctatttta aagaatgata aaacgaatat
     1681 ataaagtgga actggaaggg cgttatttgc aaattgccga ttatcccggt gaaaaaggga
     1741 cgattatcgc gctccatggc ctgacaggaa cccataagaa catgctccat tatgcgcaaa
     1801 gcttgcaggg ggactatcgg ttcatcgcct gcgatctaag gggaaggggc gacagttcgg
     1861 ggatggatga cgtcccctcc atcttctccc acgccgaaga cgttgaagaa ttgattgccg
     1921 tattggggat aaaacaaccg attttgcttg gccattcaat gggtgccttc atatccgcga
     1981 ttgtggcaag cgaattgcca tcggtgaaag gactcgtctt gctggacggc gctgcgcgga
     2041 tgtcgggtcg ccagcatgag attgtcagac cgtcgctcgg gaggctccgc aagacgtatg
     2101 aatcgaagcg tgcatatgtc gaggagctga agcagctgta caaacggtta ggcatcaagt
     2161 ggaccgacgc cgtccaggaa attgtggagt acgaaatcca tggaacaggc aatggatgga
     2221 tgcacaagtc tgcgaatgaa aacatcgaag aggactttgc gagctttgac tttttcaacc
     2281 cgatcaaagt gatggggaaa atcacttgca aaacattgct catccaggcg gacgggggaa
     2341 tcggcacatt cccgccgttc ttcacggatg ccgattacga agagacgaaa aaatacacat
     2401 cgaaactgac gacggtcaat tcggatagca atcattacac catggtcttt gagaaacagg
     2461 aagctatttt agatgaaatc cggaagttct tggatggagt gatcttggat gagtgaagga
     2521 ttggcttatt tcaaggaagt gtatggcacg gttccgggat gggtcggaaa aatgcatgat
     2581 tacaggccgg acgcgcttga tcattacaca agcttgcgca gcgccatcat ggaggacggg
     2641 cttttatccc ggaaggaaaa agaccttctc cttgtgggaa tcaatgcagc aagatgctat
     2701 gaacgaagca tggtctacca cacgaaaggt gcgatcgatg gaggtgccac catcccggag
     2761 ctgatcgagt acttgcttgt cgcgtaccga tatgggggac ttcagtcaat gcaaaccggc
     2821 atccaatcgg taaagtacgc acttgctttg aatggattgc cggagggaaa ggtaccttac
     2881 ggtatttgta acgagcacat tttgagttat ttcatttccg tagtagatga agaggatgca
     2941 tggtttgtat caaacctatt gaaggcgcca gaggaactgt ttaacgattt gctgttagta
     3001 agagggctcg tcagtgcaaa gatgaaattc cttctgatgg tcggcatcca tagcaccgcg
     3061 ctgcaaggaa cggaagcggg gaaatggatg gagaaggccc gggggagtgg agcgacggaa
     3121 ggcgagttgg cggaagtcgg tctcatctgc ctgttgaccg cgggcattcc ggcgtggttc
     3181 gaggctagcg actccttacg ggaaggggtc ggctaaaatg gccatcgtct atgaacgggt
     3241 tgaaaatgcc ctatctgcaa ttaaaagcgg gatgacgatc atggtcggcg gattcggcct
     3301 cgtcggcgcg ccgctcacgt tgattgaagg tctaacggag aaagacatcg aagggctcac
     3361 aatcatcagc aataatcttg gtgaagcggg agaagggctt ggcatgctgc tccgtcagca
     3421 gaaaatacga aaagcgatcg gctcctattt tacgag
//
LOCUS       DASULI010000395         3735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_208635, whole
            genome shotgun sequence.
ACCESSION   DASULI010000395 DASULI010000000
VERSION     DASULI010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208635"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2196)
                     /gene="pcrA"
                     /locus_tag="VFX34_08510"
     CDS             complement(<1..2196)
                     /gene="pcrA"
                     /locus_tag="VFX34_08510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEX5564988.1"
                     /translation="MKSISENLLAGMNPEQARAVKITEGPLLIMAGAGSGKTRVLTHR
                     IAYLVVEKGVYPSNILAITFTNKAAREMRDRIDGLLGAGTGERMWVSTFHSMCVRILR
                     RDIDRIGYSKSFSILDASDQLTVIKNVQKQLNIDPKQYDPRAMLNAISSAKNECIDAS
                     TYRGQINEFNPFERTVADVYEGYEKRLRSNQSLDFDDLIMVTLQLFEKVPEVLEFYQN
                     KFQYIHVDEYQDTNNAQYRLVNQLANKFRNLCVVGDSDQSIYRWRGADIQNILSFEKD
                     YPNAEVILLEQNYRSTKRILRAANDVIQNNRSRYDKRLRTENDEGEAIYIYKAADEKD
                     ESQFVVGKILELMKQENRKLSEFAILYRTNAQSRVIEEYLLKSNLDYTIVGGTKFYDR
                     KEIKDLLAYLRLIANNDDDLALARIINEPKRSIGNTTFERIASYAIEHDRSIFDALNE
                     VDFMGLGPRAANQVAGFRDLIKGFTQMQEFLSVTELVEQVLEKSGYREMLQNERTIEA
                     ESRLENIEEFLSVTEAFEKRAEEEDGDKSLVAFLTDLALIADIDSLDKEETASSDRLV
                     LMTMHAAKGLEFPVVFIIGMEENVFPHSRSLGDDEEMEEERRLAYVGITRAEQALYLS
                     CAQFRTLYGRSNFNSPSRFLKEISEEVTEVLSSATGMDFNQESRIQVRTPVRKPVQRP
                     AYKESGGEKLGWRAGDKAVHKTWGEGTVVSVKGSGEEIELDIAFPQPVGI"
     gene            complement(2230..2940)
                     /locus_tag="VFX34_08515"
     CDS             complement(2230..2940)
                     /locus_tag="VFX34_08515"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HEX5564989.1"
                     /translation="MDFRTWRHAFKLDPAKTVTDEQLEQLCESGTDGIIVGGTDGVTL
                     DNVLDLLVRVRRYSVPLALEVSTVDSVTPGFDYYFIPTVLNSSKTEWINGIHHAALRE
                     YGHMMNWEEIIPEGYCIMNPDCKAAELTAANTDLDEEDVIAYARMAEHLFKLPVFYLE
                     YSGTIGDKELVRSAKEVLENTRLFYGGGIRNAAEAEHMAEVADTVIVGNIVYEDLKAA
                     LETVQAVKKNSPGETSGV"
     gene            3201..3713
                     /locus_tag="VFX34_08520"
     CDS             3201..3713
                     /locus_tag="VFX34_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5564990.1"
                     /translation="MSSEKWFDEYAGMVYSYILLLVRNEHIAEDLTQETFMKVVANEQ
                     AFKGESSVKTWIFRIAYTTTMSHFRKEKPFATLFDVTIVDSNDAFSPEKALFQTSLQK
                     QFYDALSTLKPSYRQVILLRKINGFSTKETSYILKCSEGNVKMRLTRALAAFKKELEN
                     GGITSETFIG"
BASE COUNT          949 a          934 c          796 g         1056 t
ORIGIN      
        1 aatgccaacc ggttgaggga atgcaatgtc aagttcaatc tcttccccag aaccttttac
       61 gctgacaact gttccttcac cccacgtttt atgaaccgct ttgtcacctg cacgccagcc
      121 gagcttttct ccaccgctct ccttgtatgc tggacgctgc accggcttac ggaccggcgt
      181 tcgtacttgg attcgacttt cctgattgaa atccattcct gtagcactcg aaagcacttc
      241 cgttacttcc tcggaaatct ctttcaggaa gcgggacgga ctattaaagt tcgaacgacc
      301 atataacgtt ctgaattgcg cacacgataa atatagcgcc tgctccgcac gggtaatgcc
      361 tacataggct agacgccgct cctcttccat ctcctcgtca tccccaagtg agcgggaatg
      421 cgggaagacg ttctcctcca tcccgataat gaagacaacc gggaactcca atcccttggc
      481 tgcgtgcatc gtcatcaaga caagccgatc ggatgaagcg gtttcctctt tatcaaggga
      541 atcgatatcc gcaatgagtg cgagatccgt gaggaatgct acaagtgatt tatcgccgtc
      601 ctcttcttcc gcccgtttct cgaatgcttc cgtaacggac aagaattcct caatattctc
      661 gagtcggctt tccgcttcaa ttgtccgttc gttttgcagc atctcacggt agccggactt
      721 ctcaagaacc tgttccacaa gctctgtcac ggacaagaat tcctgcattt gtgtaaatcc
      781 tttaatgagg tcacggaagc cggcaacttg attggctgca cgaggaccga gacccatgaa
      841 atccacttca ttcaacgcgt caaagatgga gcggtcatgt tcaatcgcat aggaagcaat
      901 ccgctcaaat gttgtattcc cgatgcttcg cttcggttcg ttgataatgc gggcgagtgc
      961 gaggtcatca tcgttatttg caatcaaccg gagatacgca agaaggtcct tgatctcttt
     1021 ccggtcatag aactttgtac cgccgacaat cgtatagtcg aggtttgatt tcaacaaata
     1081 ctcctcgatt acacgggact gcgcatttgt tcgatataaa atagcgaatt cgctaagctt
     1141 tctgttttcc tgtttcatca gctcgaggat tttgcctaca acaaactgcg attcgtcctt
     1201 ctcatctgca gctttataaa tgtagatggc ttccccttcg tcgttctccg tccgaaggcg
     1261 tttatcatac cggcttcgat tgttttggat tacatcattc gccgctctca aaatacgttt
     1321 tgtcgagcgg tagttctgct caagtaaaat cacttctgca ttcggatagt ccttttcgaa
     1381 ggacagaata ttttggatgt ccgccccccg ccaacggtaa atcgactggt cggagtcgcc
     1441 gacaacgcac agattccgaa acttattggc aagctggttc accaatcggt attgcgcgtt
     1501 gtttgtatcc tggtactcgt ccacatggat gtactggaat ttattttgat aaaactccag
     1561 aacctctgga accttttcaa ataactgcaa cgtcaccatg atcaaatcat cgaaatcgag
     1621 tgattggttg gagcgcaggc gtttttcata gccttcatat acatctgcaa ccgttctttc
     1681 aaacggattg aattcattga tttgcccacg atatgtactt gcgtcaatgc attcgttctt
     1741 cgcactgctg attgcattca gcatcgcacg aggatcgtat tgcttcgggt caatgttcaa
     1801 ttgtttctgg acatttttaa tgaccgttag ctggtctgat gcgtcaagaa tcgagaacga
     1861 cttggaatag ccgattcggt cgatgtcccg gcgaaggatc cggacacaca tggagtggaa
     1921 tgtcgacacc cacatgcgtt cgcctgtacc cgcgcctaat agcccatcaa tacgatcccg
     1981 catttcccga gctgctttat tcgtaaacgt aatcgccaaa atattggagg gatagacccc
     2041 tttttccaca accaaatacg cgattcgatg tgtcaaaacg cgtgtcttac cagaaccagc
     2101 tcctgccatg ataaggagtg gcccttctgt tattttcacc gctcgtgcct gttcagggtt
     2161 catgccggca agtagatttt ctgaaatgct tttcatgcca taccgtcctt tcggaacatt
     2221 tgttctctat tatacacccg atgtctctcc cggtgaattc ttctttacag cctgtacagt
     2281 ttccaaagcc gcttttaaat cttcataaac gatattcccg acaatcactg tatctgctac
     2341 ctcggccatg tgctcagctt ccgcagcatt tcggattcct ccgccataaa aaagcctcgt
     2401 gttttcgagc acttcctttg ccgaacgaac gagttcttta tctccgattg tcccgctata
     2461 ttcaagataa aataccggca gcttgaatag atgctctgcc atgcgcgcgt acgcaattac
     2521 atcctcttca tcaagatccg tattggccgc tgtcagctcc gccgccttgc aatccggatt
     2581 catgatgcaa tagccctccg gaatgatttc ctcccaattc atcatgtgtc cgtattcacg
     2641 caaagctgca tgatggatcc cattgatcca ctccgtcttg gaggagttca ggactgttgg
     2701 gatgaaatag taatcaaacc ctggtgtcac tgagtcgact gtcgatactt ccaatgcaag
     2761 tggaacagaa tagcggcgta cacggacaag aagatccaac acattgtcca atgttacacc
     2821 gtcagttcct cctacaataa tgccgtccgt gccagattcg catagctgtt ccagctgctc
     2881 gtccgtcact gtttttgcag gatctaattt aaaggcatgc cgccaagttc taaagtccat
     2941 atttctcacc cgtctcgtca atgtacgttc attcattata gcaaaaaacg aggcagattg
     3001 ttctctcccc ggccatgtta tgtgaaaagt catggtcatc atttggtttg tttgatttcg
     3061 attgtctgac attacaagat tgccctattt ttccacgctt gcaaccgtga taatagatgt
     3121 acgaaagaat tttcagagaa atcgttaccg atttggtcat tgtgcgtcta aggggtgcag
     3181 gaaacaaaag gggtgatgag gtgtccagtg agaaatggtt cgatgaatat gcagggatgg
     3241 tctatagcta tatcttatta ctcgttcgaa atgaacacat tgccgaagac ttaacccaag
     3301 agacgtttat gaaagttgtt gccaacgagc aagcattcaa aggagaatcc tcggtaaaaa
     3361 catggatttt cagaatcgcc tatacaacaa cgatgagtca ctttcgaaaa gaaaaaccgt
     3421 tcgcgacttt atttgatgta acgatcgttg attcaaatga tgcgttttct ccagaaaaag
     3481 cgcttttcca aacgtccttg caaaaacaat tttatgatgc cctttccacc ttgaaaccga
     3541 gttatcgaca agtcattctt ctacggaaaa tcaatggctt ctcgacaaag gagacgtcct
     3601 atattttaaa atgctcggaa ggaaatgtga agatgaggct gacaagggcg cttgccgcct
     3661 tcaaaaagga attagaaaac ggagggatta cgagtgagac atttatcgga tgaggaaatg
     3721 gaaaaagagt ttgag
//
LOCUS       DASULI010000396        14918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_810579, whole
            genome shotgun sequence.
ACCESSION   DASULI010000396 DASULI010000000
VERSION     DASULI010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 14918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14918
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810579"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFX34_08525"
     CDS             <1..398
                     /locus_tag="VFX34_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5564991.1"
                     /translation="GRVLEMLEKEGLITLDESVDKVKAELSDIVDNPKNLKFEPDYEA
                     ALLPTLYNNNEGDAVLINSNYAIDAGLNPLEDSIAIEESDSPYVNVITVRSGDENNEA
                     IKALVEVLQSDEIQEFILKEWGGSVVPVK"
     gene            557..1756
                     /locus_tag="VFX34_08530"
     CDS             557..1756
                     /locus_tag="VFX34_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019381239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX5564992.1"
                     /translation="MKWKIGLIWRIIIAIGLAVGLGMLLPEIHEGFAKWFTRLFATFN
                     MIFGGFLNFVVPFIIIAFIAPGIAKLGSGSGKLLGLATVLAYASTIAAGILAFFAAMT
                     ILPNFIGGIGSESVENAGKAAAEAFFELEMTPIMGVMSALLLAFIFGIGMASIGSKTM
                     LSFFEELNVLIEKVISFIIIPLLPIHIFGIFLNMTFSGQVAKVLSVFSLVFVMVIALH
                     LLMLVIQYTVAGTLSKKNPFFLMKTMAPAYMTAIGTQSSAATIPVTLRQAKKTGASEK
                     VTDFTVPLFATIHLSGSTITLVSCSIGVLLMNGMPVSFSSYLPFILMLGVTMIAAPGV
                     PGGAVMAAVGLLSTMLGFDESMVALMIALYMAQDSFGTATNVTGDGALAMIVDRLTPK
                     VKGVETE"
     gene            2252..3031
                     /gene="sufC"
                     /locus_tag="VFX34_08535"
     CDS             2252..3031
                     /gene="sufC"
                     /locus_tag="VFX34_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX5564993.1"
                     /translation="MATLEIKDLHVEIEGKEILKGVNLTINTGEIHAIMGPNGTGKST
                     LASAIMGHPKYEVTSGSVLLDGEDVLEMEVDERAKAGLFLAMQYPSEITGVTNADFMR
                     SAINARREEGDEISLMKFIRELDNKMDVLEMDQDMATRYLNEGFSGGEKKRNEILQLM
                     MLKPKFAVLDEIDSGLDIDALKVVSNGINEMRGEGFGCLIITHYQRLLNYITPDFVHV
                     MMQGRVVKSGGAELAKQLEESGYDWIKEELGIEDETVGQEA"
     gene            3050..4354
                     /gene="sufD"
                     /locus_tag="VFX34_08540"
     CDS             3050..4354
                     /gene="sufD"
                     /locus_tag="VFX34_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5564994.1"
                     /translation="MTVETKMALTEQDVRSYSANMNEADWMAEFRTDALAKVEQLPMP
                     TPDKTKIDKWNFVDFPAHAVESTTYASIADLPAEAKELVEENQSNLYVQHNNTPAFLS
                     LSDELKAKGVILTDIFTASREHADLLKKYFMTDGVKVDEHKLTALHAALINGGVFVYV
                     PKDVVVEEPLQVLFLHDDAQASLFNHVIVVAEANSSVTYVENYLSTVEEAAGQANIIA
                     EVFAGDNAKVIYGAVDVLAKGFTTYVNRRGVAGLNSRIEWALGLMNDSDTISENITHL
                     VGNGSSSDMKTVVVGRGSQRQNFTSEIVHWGLDTDAFILKHGVMKESASSIFNGIGRI
                     AKGATRANAVQESRILMLSEKARGDANPILLIDEDDVTAGHAASVGRVDPLQLFYLMS
                     RGISQQEAERLVIHGFLAPVVSKLPIEGVKKQLTEVIERKVR"
     gene            4354..5574
                     /locus_tag="VFX34_08545"
     CDS             4354..5574
                     /locus_tag="VFX34_08545"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5564995.1"
                     /translation="MLSKEIRKHFPILDQEINGHPLVYLDSAATSQKPRQVIEAINDY
                     YAFDNANVHRGVHTLGNRATDHYEGAREKVRKFINAKSTQEVIFTRGTTTALNMVAQS
                     YGRANVGEGDEIVITHMEHHSNIIPWQQLAKEKGAVLKFVVLEEDGTLSLDKVREAVT
                     DRTKIVSIMYVSNVLGTMNPIKEITEIAHAHGAVMVVDGAQAAPHLKLDVQKLDCDFL
                     AFSGHKMCGPTGIGVLYGKKELLNEMEPVEFGGEMIDFVGLYESTWKELPWKFEGGTP
                     IIAGAIGLGAAIDFLEEIGLDQIERHEHELAGYAMEKMSSMDGLTIYGPHDPKKRAGI
                     VTFNLDNVHPHDLATVLDMNGIAVRAGHHCAQPLMKWLECSATARASFYVYNTKEDVD
                     RLVEGLRIAKEYFQ"
     gene            5574..6020
                     /locus_tag="VFX34_08550"
     CDS             5574..6020
                     /locus_tag="VFX34_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX5564996.1"
                     /translation="MPTNNLDQLYRSVIMDHYKNPRNKGVLEENNVTIDMNNPTCGDV
                     IHLTLKVEDGIVQDAKFEGEGCSISMASASMMTQIIKNKDVDTAVKYAHLFSDMMLGK
                     EIDDSIDFGDIEALSGVAKFPARIKCATLAWKAMEKGIGEESESGQ"
     gene            6095..7492
                     /gene="sufB"
                     /locus_tag="VFX34_08555"
     CDS             6095..7492
                     /gene="sufB"
                     /locus_tag="VFX34_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5564997.1"
                     /translation="MAKKMPEIGDYKYGFADKDVSVFRSERGLTREIVEEISRMKEEP
                     QWMLDYRLKSLEIFYSKPMPQWGGDLNSLNFDEITYYVKPSEATERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMKSELEDMGIVFKDTDSALRENEELFKKY
                     WGTVIPNTDNKFAALNSAVWSGGSFIYVPPGIKVETPLQAYFRINSENMGQFERTLIV
                     VDEGASVHYVEGCTAPVYTTNSLHSAVVEIIIKKDAYCRYTTIQNWANNVYNLVTKRA
                     VCEANATMEWVDGNIGSKLTMKYPACILKGEGARGMTLSIALAGKGQHQDAGAKMMHL
                     APNTSSTIVSKSISQHGGKVTYRGIVHFGRRADGARANIECDTLIMDKLSTSDTIPYN
                     EILNDNISLEHEAKVSKVSEEQLFYLMSRGIPELEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            8427..9287
                     /locus_tag="VFX34_08560"
     CDS             8427..9287
                     /locus_tag="VFX34_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5564998.1"
                     /translation="MIQVGLTGWGDHPDVYSPNSRKNEKLIDYSAHFPIVELDASFYA
                     IQPERNIRKWIDETPDDFQFIVKAYQGMTGHQRGKLPYSTPEEMFQQFKLSVDPMRAA
                     GKLAMILVQFPPWFDCIKENVDQIRYIRTQLEGYDVAIEFRHQSWYAPSMREGTLSFL
                     EDHNFIHTVCDEPQSGVGSVPLIAETTRNDKVFVRLHGRNVNGWRNTTGDNEAWRKVR
                     YLYNYADNELEEIREAVHKLESETENVFVIFNNNSGGHAAENAKRFQKMMGIDFQALA
                     PKQLDFFEEE"
     gene            9290..10114
                     /locus_tag="VFX34_08565"
     CDS             9290..10114
                     /locus_tag="VFX34_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5564999.1"
                     /translation="MAFLLLAIIGAISGIMGSIVGLGGGTILVPLTLLVGINFGWIPG
                     ITPQSVVGISVIMMVFNGLSSTLSHMKAKTVDFRTGWIFFAGSIPGLMIGAFVNKGMD
                     LPSFNLYFGILLIVLSILLLARKYLKPIQWFVRHGKQMTFIDTHGKTHVYGYPIWFAL
                     VLTFIIGFASGLFGIGGGTMIVPALILLFLFPPHIAVGTSMFIVFLSAIVNSATHISL
                     GHVPWLYALPVIPGAYIGGTIGTMLNKKMNSDTLVLVLRIMLLLFGVRSIIDGIWG"
     gene            10118..11533
                     /locus_tag="VFX34_08570"
     CDS             10118..11533
                     /locus_tag="VFX34_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEX5565000.1"
                     /translation="MRRREESIHLYHTNDIHSHFDSWPKISRYLHIQRQQHELNHEDC
                     FIFDIGDHVDRSHPFTEGTSGKGNVALLNRAGYDAVTIGNNEGITMSKTALNSLYEGA
                     DFDVILCNLTDTDGSCPKWLKPYRIYETASGIRVGVIGATAMYTDFYAKLGWRIEEPI
                     QELKKVAEQILHRTDVIICLSHLGVNEDRLLAKECGLIDVILGAHTHHLFPEGEMVNG
                     TLLAATGKFGEYVGHIELRMDMDKKKIVAKSAAVVRVSGLESREEDVQEVNVLIQAGN
                     ETLEENIFYTASPLKQQLFVDSPMSSFFGRALVDYTGADCAMFNAGIFLGSLEKGWVT
                     KRMVHGLLPHPINPCTVTLDGAELLEVYRLSNNESWPETEIKGLGFRGTYMGKMIFER
                     LFRTDDGELYAGNRKVVPGKTYTLATLDMFTFGFFFPLLKGASKEYHMPELIRDVFIW
                     YGMKRDSRIDPGTRNAGYKRE"
     gene            11671..12387
                     /gene="yunB"
                     /locus_tag="VFX34_08575"
     CDS             11671..12387
                     /gene="yunB"
                     /locus_tag="VFX34_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HEX5565001.1"
                     /translation="MPLIFPAFLVTIALFLYFVNSRLTPVYLSYAEIQTENIAAHVIT
                     QAIKSRSTEIYDVKEIMENVPNDSQGMVANTLNAEIINKTRAEIHGLVESHLKMAESG
                     KLEMLPLSENIEYDPKAMDGSGGVVFFVPMGQAANIPLIGNFGPKIPIRFHVIGEVQT
                     NVETEITEFGINNAMIEVNILVKVNVQIVVPLATRRSVVEQRIPVAIGLIQSPIPQIY
                     NGGGGNPPQIEVPFNPGGGQ"
     regulatory      12441..12616
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            12758..14335
                     /locus_tag="VFX34_08580"
     CDS             12758..14335
                     /locus_tag="VFX34_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEX5565002.1"
                     /translation="MIGTWVSILPPIVAIVMVLATKRVLLSLGSGIVTAALLVASFAP
                     VDTLKNLWTAMIVSFWSEGELNTYNIFIMIFILLLGVITAFVSLSGGSRAFADWAVKR
                     IKTRRGAKLLTVLIGILIFVDDYFNSLAVGQIARPITDQHRISRAKLAYFIDSTSAPI
                     CVVSPISSWGAFLIGQLALIFGTSAAISYSPLSAFLMMAPMNFYVIATLAMVFFFAWT
                     NIDYFEMKKHETRAQENGELFNPDKEIPGQLKEDFPVHSHGRVIDLIAPIITLVVVTL
                     GMMIWTGYRIGGSASIWTIFENTDVPMSLVVGGLAGTLLAAALYISQMGKNETASYGL
                     VSKAFISGAKAMMPAILILIFAWALTHLIGVLETGPFLSSVVEQSNVPVNFLPVIMFI
                     LAGLMAFSTGTSWGSFGILLPIAGTIMINAEPELLLPALSAVLAGAVFGDHCSPISDT
                     TILSSTGAGSNHMDHVSTQLPYALISAGIAAIGYFILGLTGSVWIGLVAVIILIAGLF
                     TVWTSKRNKAEVKATSV"
     gene            14613..>14918
                     /locus_tag="VFX34_08585"
     CDS             14613..>14918
                     /locus_tag="VFX34_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX5565003.1"
                     /translation="MEQRSQWGTRAGFILAAVGSAVGLGNIWRFPYVAYENGGGAFFI
                     PYLFALLTAGIPILIMEFTIGHKYRGSAPLSFFRMKGKGAEWLGWWGIFVSFVISTYY
                     "
BASE COUNT         4143 a         3245 c         3689 g         3841 t
ORIGIN      
        1 atggccgtgt gcttgaaatg cttgaaaaag aaggattgat tacacttgat gaaagtgtcg
       61 ataaagtaaa agcagaactc tcagatattg tggacaatcc gaaaaacttg aagttcgaac
      121 cggattacga agctgcatta cttccaactc tatacaataa taatgaaggc gatgctgtgt
      181 tgatcaactc taactatgcg atcgatgccg gtttgaaccc gcttgaagat tcgattgcaa
      241 ttgaagaaag tgattctcca tacgtaaacg tcattacggt ccgttctggt gatgaaaaca
      301 acgaagcgat caaagcgctt gttgaagtgt tgcaatccga tgaaatccag gaattcattt
      361 tgaaagaatg gggcggctct gtcgttcctg ttaaataatt gagtacagcc gtctgtttcc
      421 gttttagcgg agacaggcgt ttttttatgc tcccataaag tattaaaatc cataaaattg
      481 ttcccatttt ttcttcacta tggtaaagtg ggattattgt ttttgtaaac aattgtacat
      541 ttgaggagga catattatga agtggaagat aggccttata tggcgaatca taatcgccat
      601 agggcttgcg gtggggcttg gaatgttgct cccagaaata catgaaggtt ttgcaaaatg
      661 gtttacccgt ttgtttgcaa ctttcaacat gatcttcggc ggttttctta actttgtcgt
      721 accgttcatc atcatagcgt tcattgcacc aggcatagca aaactgggca gcggatccgg
      781 taaacttcta ggattggcaa ccgtgcttgc ttatgcatcc acgattgcgg cagggatctt
      841 agcgtttttt gcagcgatga cgattctgcc gaatttcatt ggcggcatcg gctccgaatc
      901 tgttgaaaat gcggggaaag ccgctgctga agcctttttt gaacttgaaa tgacaccaat
      961 catgggcgtc atgtcggcat tgctgcttgc gttcattttc gggatcggga tggcgtcgat
     1021 tggaagtaag acaatgcttt cattctttga agagctgaat gtgcttattg aaaaagtcat
     1081 ttcattcatc atcatcccat tattgccgat ccatattttc ggcattttcc ttaacatgac
     1141 attcagcgga caagtggcaa aggtcttatc ggtattctca cttgtcttcg tcatggtcat
     1201 tgctttgcat ctgcttatgt tggtcattca gtatacagtt gccggaaccc tttcgaagaa
     1261 gaatccattc ttcctgatga agacaatggc gcctgcctat atgacagcga ttggaaccca
     1321 atcatcggct gcgacgattc cggtcacatt aagacaagct aaaaagaccg gagcatcgga
     1381 aaaagtgacg gacttcaccg tgccgttgtt tgcaacgatc catttgtcag gaagtacaat
     1441 cactctagta tcctgctcga tcggagtcct gcttatgaat ggcatgccgg tcagtttctc
     1501 aagctacctg ccattcatcc taatgctcgg tgtaacgatg attgcagccc caggagtccc
     1561 tggaggagcc gtcatggctg cagttggcct tctcagcaca atgctcggct ttgacgaatc
     1621 gatggtcgca ctgatgattg ccctctacat ggcacaagac agcttcggca ccgccacaaa
     1681 cgtaaccggc gacggagccc tagccatgat cgtcgaccgc cttactccaa aagtaaaagg
     1741 tgtggaaacg gaataatatt taatttgaaa agtagaagag ggaatgtgac aattctctcc
     1801 ttctactttt taatttgatc taaagaacct tgattaggat tgtagcgaaa gccgttacga
     1861 agaacggctt ttgcgagtaa aattgcagcg ttgcgagcag gaatgccaaa acgctgcctt
     1921 aatttctgca gaagcgtaga aattaaggca actcgaaccc ttcgctgttc gattggaaag
     1981 cgaccgcctg tagcaaaaat ccgggttctg tcaaagctcg ttgttggaaa tgaatagaac
     2041 tattttcatt gattctcagt aagagcggga taattgaaaa tgggctgcct tttaaattat
     2101 aagaaaatac gtggcaatac tcgtagattc cccataaatt gataataatc ctcaatgatt
     2161 tgcaacaacg tgccgaatga gctaaaatta gaatgatact aaatagagaa tggtttaccc
     2221 cgttcatctt tatcatattg gaggtattgg aatggcaact ttggaaatca aagaccttca
     2281 cgtcgaaatt gaaggtaaag aaatattgaa aggcgtcaac ctaacaatca atacaggtga
     2341 gatccatgcg atcatgggac ctaacggaac cggtaaatcg acacttgcat ccgcaatcat
     2401 gggccacccg aaatatgagg taacttccgg ctctgtcttg ctagacggcg aagatgtcct
     2461 tgaaatggaa gtggatgagc gcgcaaaagc aggacttttc cttgcaatgc aatacccaag
     2521 tgaaatcact ggcgtaacaa acgcggactt catgcgctca gcaatcaacg cgcgccgtga
     2581 agaaggcgat gaaatttccc ttatgaaatt catccgtgaa ttggacaaca agatggacgt
     2641 ccttgaaatg gatcaagata tggcaacccg ttacttgaac gaaggattct caggcggaga
     2701 gaaaaaacgc aatgaaatcc tccaattgat gatgttgaaa ccaaaattcg cggttctcga
     2761 tgaaatcgac tccggacttg acatcgatgc attgaaagtc gtttccaacg gaatcaacga
     2821 aatgcgcgga gaaggattcg gatgcctgat catcactcac taccaacgcc ttctcaacta
     2881 catcacacct gactttgtcc acgttatgat gcaaggcaga gtcgtcaaat ccggcggagc
     2941 ggaacttgcg aagcaacttg aagagagcgg ttatgactgg atcaaggaag aactaggaat
     3001 cgaagacgaa acagttggac aagaagcata agaaagggga cgattcaaaa tgacggttga
     3061 aacaaaaatg gcattgaccg aacaggacgt ccgctcttat tccgcaaata tgaatgaagc
     3121 cgattggatg gcagaattcc gcacagatgc gttagcgaaa gtggaacagc ttccgatgcc
     3181 aacaccagat aaaacgaaaa tcgacaaatg gaactttgta gatttccctg cccacgcagt
     3241 ggaaagcaca acatacgctt ccatcgcaga cttgcctgca gaagcgaaag aattggtcga
     3301 agagaatcaa agcaatctat atgtacagca taacaataca cctgcattcc tttccctttc
     3361 agatgaattg aaagcaaaag gcgttattct tacagatatt ttcactgctt cacgtgaaca
     3421 cgctgacctg ttgaagaaat atttcatgac ggatggcgtg aaagtggatg agcataaatt
     3481 gactgcgtta cacgcagcgc ttatcaacgg cggcgttttc gtctatgtgc cgaaagatgt
     3541 tgtcgttgaa gagccgcttc aagtgctgtt tttacacgat gacgcacaag catcactctt
     3601 caatcatgtg attgtcgtag ctgaagcgaa cagctctgtg acatatgtag aaaactatct
     3661 gtctacagtc gaagaggctg caggacaggc gaatatcatc gcggaagtat tcgcgggtga
     3721 taatgcgaag gtaatctatg gcgcagtgga cgtccttgcc aaaggattca cgacatacgt
     3781 caaccgccgc ggagtagctg gtttgaacag ccgcatcgaa tgggcgttag ggttgatgaa
     3841 cgacagcgat acaatttcag agaacatcac gcacctcgtc ggaaatggtt cttcaagcga
     3901 tatgaagact gtcgttgtcg gccggggaag ccaaaggcag aacttcactt ctgaaatcgt
     3961 ccactgggga cttgatacgg atgcattcat ccttaagcac ggcgttatga aagaatccgc
     4021 ttcttcaatc ttcaacggca tcggcagaat tgccaaaggt gctacaagag cgaatgcggt
     4081 tcaggagtca cgcattttga tgctaagtga gaaagcacgc ggagacgcga acccgatcct
     4141 attgatcgat gaagatgatg taactgcagg ccacgccgca tcagtcggcc gtgtggatcc
     4201 gcttcaattg ttctacttga tgagccgtgg aatttcacaa caggaagcag aacgccttgt
     4261 tatccatggt ttcctagcac ctgtcgttag caaattgcct atcgaaggcg tcaagaagca
     4321 attgacggag gttatcgaaa ggaaagtgcg ctaatgctca gcaaagagat ccgcaaacat
     4381 ttcccgatac ttgaccagga gatcaacggg cacccgctcg tttatttaga tagcgccgcg
     4441 acttcccaaa agccgcggca ggtcattgag gcgatcaatg actactatgc gttcgataat
     4501 gcgaacgtcc atcgcggagt ccatactttg ggcaatcggg ctactgatca ttatgaaggg
     4561 gcgcgtgaaa aagtccgcaa gttcatcaat gcgaaatcga cgcaagaagt gatcttcact
     4621 cgcggtacaa caacagccct caacatggta gcccaaagct atgggcgtgc gaatgtcggc
     4681 gaaggcgatg aaatcgtgat cacccatatg gaacaccatt ccaatattat tccgtggcag
     4741 caattggcga aggaaaaagg tgcagtcctg aagtttgttg ttttagaaga ggacggcaca
     4801 ctctcccttg ataaagtgcg tgaagcagtg acggatcgca ccaagatcgt atcaatcatg
     4861 tatgtttcca atgtgctcgg cacgatgaat ccgatcaagg aaatcacaga gattgcacat
     4921 gcacatggcg ccgtcatggt cgtcgatgga gcgcaggccg cccctcatct gaaattggat
     4981 gtccaaaaat tggactgcga ttttctcgct ttttcaggcc ataaaatgtg cggccctacg
     5041 gggattggtg tcctttatgg aaagaaagag ttattaaacg agatggagcc ggttgaattc
     5101 ggtggtgaaa tgatcgattt tgtcggtctt tatgaatcca cttggaaaga acttccgtgg
     5161 aaattcgaag gaggaacgcc gatcattgct ggagcaattg gactcggtgc cgccatcgac
     5221 tttttggaag aaatcggttt ggatcaaatc gagcggcatg aacacgaact tgcgggttat
     5281 gcgatggaga aaatgtcatc aatggacggt ctgacgattt atggaccgca tgatccgaag
     5341 aagcgtgcag gcatcgtgac gttcaatttg gacaatgtcc atccacatga tcttgccacg
     5401 gtactagata tgaacggaat cgcagtgcgg gcaggtcacc attgtgcgca gccgcttatg
     5461 aaatggcttg aatgttcagc gacagcccgt gcaagcttct acgtgtataa cacgaaagaa
     5521 gatgttgacc gtctcgtaga aggactccgt attgcaaagg agtatttcca ataatgccta
     5581 caaacaattt agaccagcta tatcgttcag tcatcatgga tcattataag aatccaagga
     5641 ataaaggtgt actggaagag aacaacgtca cgatcgacat gaacaatccg acgtgtggag
     5701 atgtcatcca tctgaccctg aaagtcgagg atggcatcgt gcaagatgca aagtttgaag
     5761 gggaaggctg ttcgatctcc atggcatccg catcgatgat gacgcaaatc atcaaaaata
     5821 aagatgtcga cacggctgtg aagtatgccc atttgttttc ggatatgatg ctcggaaaag
     5881 aaatcgacga ctccatcgac ttcggcgata tcgaagccct ttccggagta gccaaattcc
     5941 ctgcacgcat caaatgtgca acgcttgcgt ggaaagcgat ggaaaaagga atcggggaag
     6001 aatccgagtc cgggcaatga atccaaaaac ggttgcctgg agttttccgg gcaactgggg
     6061 caagcaatac aaatgaaaga acggaggaat attgatggct aagaaaatgc ctgaaattgg
     6121 cgattacaaa tatggtttcg cagacaagga tgtatctgtt ttccgttcgg aacgtgggtt
     6181 gacgcgtgaa attgttgaag aaatttcgag gatgaaagaa gagccacaat ggatgttgga
     6241 ctaccgtctg aaatccctgg aaatcttcta tagcaaaccg atgccacaat ggggcggaga
     6301 cttgaattct ctaaacttcg acgaaatcac atattacgta aaaccttccg aagcgacaga
     6361 gcgttcttgg gatgaagtgc ctgaagaaat caagcgcaca ttcgataaac taggaattcc
     6421 tgaagctgag caaaaatacc ttgcgggtgt ttctgcgcag tacgaatccg aagttgttta
     6481 tcacaacatg aaatcagaac ttgaagacat gggaatcgtc tttaaagata cggactcggc
     6541 tttgcgtgaa aacgaagagc tattcaagaa gtattgggga actgtcattc cgaacacaga
     6601 caacaagttc gcagctttga actctgctgt ttggtccggt ggttcattca tctatgtacc
     6661 acctggcatc aaagtggaaa cgccacttca agcctatttc cgtattaact cggaaaacat
     6721 gggtcaattc gagcgtacgt tgatcgttgt tgacgaaggc gcaagcgtcc actacgtaga
     6781 aggatgtaca gcacctgttt acacgacaaa ctcccttcat agtgcggtcg ttgagatcat
     6841 cattaaaaaa gacgcgtact gccgttacac gacgattcaa aactgggcga acaacgttta
     6901 caacctagtt acgaagcgtg cggtgtgtga agcgaacgct acgatggaat gggtagacgg
     6961 caacatcggt tcgaagctta caatgaaata ccctgcatgt atccttaaag gtgaaggtgc
     7021 tcgcggtatg acattgtcca tcgctttggc tggtaaaggc caacaccaag atgcaggagc
     7081 taaaatgatg cacttggcgc ctaatacgtc atccacaatc gtttcgaaat cgatttccca
     7141 acacggcggt aaagttacgt accgcggaat cgtccacttc ggacgccgtg cagatggcgc
     7201 gcgcgcaaac atagagtgtg acacgctcat catggataaa ctatcgacat ccgatacgat
     7261 tccatacaac gaaatcctga acgacaacat ttcactcgag cacgaagcga aagtttccaa
     7321 agtgtcggaa gagcagctct tctacttgat gagccgcgga attccggagc tcgaagcgac
     7381 agaaatgatc gtcatgggct tcatcgagcc attcacaaaa gaattgccga tggaatacgc
     7441 agtagaaatg aaccgcctga tcaagttcga aatggaaggt tcaatcggat aatcttacgt
     7501 aaacattgat gtaacaacat ttttacaaga aaaataagga gcagaaaaca ggtcgtggcg
     7561 atttcgtgcc gattcagatt ttctgcccat tattggaagc gaacttaata agttcgtctc
     7621 agactgtagt cagactccag aaaatatggg gtttgtctac agtctttttt tcaataaaga
     7681 tagtattaga aatttgattt ccgctacggg cggacgcgtt ccgcgggcac ggcttcagcc
     7741 gcttccctcg ctcatgtgct cgcaacgctt cgcttttgct cgcaaaagcc gttcttcgtt
     7801 acggctttcg ctcagtccag ggtcttcagc tcgcgctgtt cccgctggag tcgccgccct
     7861 ccacttcaat caacggaacc tattactaac aatgaatgaa aacaaaagtg gatatttgat
     7921 aaagttttcc gttactggta atcgcatatc atcgccaatt cgaactttcg ttaatccgtt
     7981 tgtttccttc atataataga aggttataca aagactaaaa ttgaaaaaat gggttgtaga
     8041 tgctttgaaa acgagtttct agggtctttt ttttattaaa ataggctatc cattacattt
     8101 ttaattgtaa ttcaacaaaa agaagggagc ttgactttga aaataataaa tatctagtcc
     8161 ctgtatgtta ttaatttcgg cactttatca aggattgaag cgaaaggtgg cgactcctgc
     8221 gggaaagcga gacaggggag accccgcagg gagcgaagcg actgaggagg ctcaccgctc
     8281 gcccgcggaa agcgtccacc tgtagcggaa atccggtttc atttcacaaa acccttttct
     8341 tctagtattg tgcaaaatgg cggaatcagc ccgctgacat atgatatgat gaaaatattc
     8401 atctacgacc ggaaaagagg gatactatga ttcaagttgg attgacgggg tggggcgacc
     8461 atccggatgt ctattcccca aactccagga aaaatgaaaa attgatcgat tacagcgccc
     8521 actttccgat cgtcgaactc gacgcttcct tttacgccat ccagccggag cgcaatattc
     8581 gtaaatggat tgacgagaca cccgacgatt ttcaatttat cgtaaaagcg taccaaggca
     8641 tgaccggaca tcaacgggga aagctgcctt attccactcc ggaagaaatg ttccagcagt
     8701 tcaagctttc cgttgatccg atgcgggccg ccgggaagct tgctatgatt ctcgttcaat
     8761 tcccgccatg gtttgattgc ataaaagaaa atgtggatca gatcagatac attcgaacac
     8821 agctggaagg gtacgatgtg gcaatcgaat tccgccatca atcgtggtac gctccatcca
     8881 tgcgggaagg gacgctttca ttccttgaag accacaattt cattcacacg gtttgtgatg
     8941 agccgcaatc cggtgttggc agcgtccccc ttatcgctga aacgacacgc aatgataaag
     9001 tgtttgtccg tttgcacggc aggaatgtca atggctggcg caatacaacc ggtgacaatg
     9061 aggcgtggcg taaagtccgc tacctctata attatgcaga caatgaattg gaagaaatcc
     9121 gtgaagcggt ccataaattg gagagcgaaa cggagaatgt gtttgtcatt ttcaataata
     9181 actctggtgg ccatgcagcc gagaacgcaa aacgctttca aaaaatgatg ggcattgact
     9241 tccaggcact tgcacctaag cagcttgact tttttgagga ggaataagta tggcgtttct
     9301 gttattggca attatcggtg caatatccgg cataatggga tcgatcgtcg gactcggggg
     9361 cggaacgatt cttgtgccgc tcacattatt ggtagggatc aatttcggtt ggattccagg
     9421 catcacgccg caaagcgtcg tcggaatttc agtcatcatg atggtgttca atggactttc
     9481 ttccacactt tcgcatatga aagcgaagac cgtcgacttc aggacagggt ggatcttttt
     9541 cgcgggctcc ataccgggct tgatgatcgg ggcgttcgtg aataaaggaa tggatctgcc
     9601 atcatttaat ttatacttcg gcatcttgct catcgtcctt tccatccttt tgcttgcacg
     9661 caaatacttg aaaccgatcc aatggtttgt cagacacggc aaacagatga catttatcga
     9721 tacccatggg aagacccatg tgtatggcta tccgatctgg tttgcgctag tactgacatt
     9781 catcatcgga tttgcttccg gcctattcgg catcggtgga ggcacgatga ttgtgccggc
     9841 actgattctc ttattcctgt tcccgccgca tatcgccgtc ggaacgtcga tgttcatcgt
     9901 tttcctgtca gcgatcgtaa actccgccac gcatatctct ttagggcatg tgccttggct
     9961 ttatgcgctt ccggtcattc cgggagccta tatcggcggt acgatcggaa cgatgctcaa
    10021 taaaaagatg aactcggata cattagtgct tgtgctgcgc atcatgctgt tattgttcgg
    10081 agtgaggtcg atcatcgatg ggatttgggg atgagccatg agacgccgtg aagaatcaat
    10141 ccacttgtac catacgaatg atatccatag ccatttcgac agctggccga aaatcagcag
    10201 gtatctccac atacagcgac agcagcatga attgaatcat gaggattgct tcatattcga
    10261 cattggtgac catgtcgacc ggtcacaccc tttcacggaa gggacatccg gcaagggcaa
    10321 tgtggcattg ctgaacaggg caggatacga cgcggtgaca atcggcaaca atgaagggat
    10381 cacgatgtcc aaaaccgcat tgaactccct ttatgaaggt gctgatttcg acgtcatcct
    10441 ttgcaactta acggacacgg atggttcatg tccgaaatgg ctgaagcctt acaggattta
    10501 cgaaacagct tccggcatcc gagtaggcgt catcggcgcg actgcgatgt atacggattt
    10561 ttatgcaaaa ttaggttggc gcatcgaaga accgatacag gaattgaaaa aagttgccga
    10621 gcaaatcctg cacagaacgg atgtcatcat ctgcctttcc catttaggcg tgaatgaaga
    10681 caggctcctt gctaaagaat gtgggctgat cgacgtcatt ttaggtgcac atacccatca
    10741 tttattcccg gaaggtgaga tggtgaatgg cacattgctt gccgcgactg gaaagtttgg
    10801 cgagtacgtc gggcatattg aactccgcat ggatatggat aagaaaaaaa tcgttgcgaa
    10861 aagtgcggca gtagtccgtg tgagcgggtt ggaaagccgc gaagaggatg tacaggaagt
    10921 gaacgtactg atccaagcgg gtaatgaaac gcttgaagag aacatcttct atactgcaag
    10981 cccattaaag cagcagcttt tcgtggacag cccaatgtcc tcgtttttcg gaagggcatt
    11041 ggtcgattac acaggggccg attgcgccat gtttaatgca gggattttcc tagggagcct
    11101 tgaaaagggc tgggtgacga aacgcatggt gcatggcttg ctgccgcatc cgatcaatcc
    11161 ctgtacagtt actttggacg gggcggaatt gttggaggtt tacagactat ccaataacga
    11221 atcgtggccg gagacggaaa tcaaagggct cggctttcgc ggcacataca tgggaaaaat
    11281 gatttttgaa agactttttc ggacggatga cggagaatta tatgccggca atcgaaaagt
    11341 ggttcccgga aaaacataca cgttggcaac gcttgatatg tttacattcg gcttcttttt
    11401 cccgttattg aaaggggcgt cgaaggaata tcatatgccg gaactgatcc gggatgtctt
    11461 catttggtat gggatgaaaa gagatagccg tatcgatccg ggtacgagga atgctggata
    11521 taaacgagaa tgaacggccc cgttcctgca taatattagg caggaggggt cgttttgaga
    11581 ttttatgggc aaccatcccg aaattataaa aaaaggaacc gctaccgacg ttccagtcct
    11641 aatgacaggc ctcgcaggaa gcggcgcctg ttgccgctca tttttccagc gttccttgtg
    11701 acaatcgccc tttttctcta ttttgtcaac tcaagactca ctccggttta tctttcatac
    11761 gcagaaatcc aaacggagaa tattgcggca catgtcatta cgcaggccat caaatcaagg
    11821 tccaccgaaa tttacgatgt aaaagaaatc atggaaaatg tgccgaatga ttcgcaggga
    11881 atggtggcga atacacttaa tgctgaaatc ataaataaaa cccgagcgga aatccacggt
    11941 ctcgtagagt cgcatctcaa aatggccgaa agcggaaagt tggaaatgct tccgctcagc
    12001 gagaatatcg aatacgatcc aaaagcgatg gatggttccg gtggagtcgt cttttttgta
    12061 ccgatggggc aagcggcgaa cattccattg atcgggaact tcggaccgaa aattccgatc
    12121 cggttccatg tcattgggga agtgcagaca aacgtagaga ctgaaattac tgagttcggc
    12181 attaataacg caatgattga agtgaatata ttggtgaaag tcaatgtgca aatcgttgtt
    12241 ccacttgcga cacggagaag cgtcgtggag cagcggatac ctgttgccat cggtttgatc
    12301 caaagtccga ttccccaaat ttataatggg ggcggaggga atcctccaca aattgaagtt
    12361 ccattcaatc ctggcggcgg acaataacgg cccttgaaaa atgccggatt tgtgttatgc
    12421 tagtataaca agaggaacac cgattaggta gaggctgcaa ggcttatgag taatggacgc
    12481 gagattggca tcgatgaccg cccgtgaaag gaagctttgc cgaagctgga atggagcgcc
    12541 gacccatcca agctggggtc attccgaata ggaatggcac tgtcatgcaa atatcgcatg
    12601 atgagctaca gatcgtacgt tagataaaac atcttagatt atcttacaaa cgaaccgtag
    12661 aataagccga cacctgatgc acatcctagt gcatgggtgt cggtttttta attcctacta
    12721 ctagttcttc aatcttaagt ttggaggaat gttttttatg ataggcactt gggtttcaat
    12781 tttgccgccg atcgtggcga tcgtcatggt attggcgaca aaaagagtat tattatcact
    12841 tggatccgga attgtgacgg ctgcattatt ggtcgccagt tttgcaccgg tcgatacgtt
    12901 gaaaaacctt tggactgcga tgatcgtctc tttctggtct gaaggggaat tgaatacgta
    12961 taatattttc atcatgatct tcattttgtt attaggggtc attacagcat ttgtcagctt
    13021 atccggcgga agcagggctt ttgcggactg ggcggtcaag cgcattaaaa cgagacgcgg
    13081 ggcaaagctg ctcactgtgt tgatcggaat cctcattttt gtagatgatt atttcaactc
    13141 attggcggtc ggtcagattg cacgaccgat tacggaccag caccgcattt caagagcgaa
    13201 attggcgtat ttcatcgatt ccacgtccgc tccgatttgc gtagtttcac cgatttccag
    13261 ctggggggca ttccttatcg gccaattggc ccttatcttc ggtacatctg cggcaatcag
    13321 ctactcgccg ctatccgcat ttcttatgat ggcaccgatg aacttttatg tcatcgcaac
    13381 tttggcaatg gtgtttttct ttgcttggac gaacatcgat tactttgaga tgaaaaaaca
    13441 tgaaacgcgc gcccaggaaa acggggagct tttcaatccg gataaagaga ttcctggtca
    13501 attgaaagag gatttcccgg tacactccca tggccgtgtg attgatttga ttgcaccgat
    13561 cattacactt gtcgttgtaa cgttggggat gatgatttgg acaggctatc gcatcggcgg
    13621 ctccgcaagt atctggacga ttttcgaaaa cacggatgtt ccgatgtcat tggtcgtagg
    13681 cggccttgcg ggaacgctat tggcagcggc tctgtacatt tcccaaatgg ggaaaaacga
    13741 gactgcttcc tatggccttg taagtaaagc attcatcagc ggtgccaagg caatgatgcc
    13801 ggcgattctc atattgattt ttgcatgggc attgacgcat ttgatcggtg tgctcgaaac
    13861 aggaccattc ctgtcaagcg tcgtggaaca gtcaaatgtt ccagttaact tcctgccggt
    13921 catcatgttt atcctagctg gtttgatggc attttcgaca gggacatcat ggggttcttt
    13981 cgggatcctg cttccgattg ccggaacgat tatgatcaat gcggagcctg aactgttgct
    14041 gccggcgttg tcagccgttt tggcaggagc tgttttcggg gatcactgtt ctccgatttc
    14101 cgatacgaca atcctttcct cgaccggtgc gggatccaac catatggacc atgtgtcgac
    14161 acagcttcca tatgcgctga tctctgcagg cattgctgca atcggctact tcatccttgg
    14221 actcacggga tcggtctgga tcggactcgt ggcggtcatc attctaatcg caggcttgtt
    14281 caccgtatgg acgtcaaaac ggaataaagc ggaagtgaaa gcgacttccg tctgatgcat
    14341 gatttactgt cacacccgcc gggcactcgg cggatgtggc ggttttttat tggaataaag
    14401 cataaactgt gccctcgaat cataaatttc cactaccgga acagttccgg attcccatga
    14461 aggagttgaa aagtcataaa tggaatgggt attttttcaa taaaaaacat tttgacaata
    14521 gatactaaga cggatatact tgtcttaaga ttatataata atctgaaaat ggtaatcttt
    14581 taacagatta ataaagggaa aggggattta ttatggaaca gcgttcgcaa tgggggacac
    14641 gtgccggctt tattctagcg gctgtcggtt cagctgtcgg gttaggaaat atctggcgtt
    14701 ttccatacgt tgcgtatgaa aatggcggag gggcattttt cattccttac ttatttgcac
    14761 tcctaaccgc aggtattccg atactgatca tggaattcac gataggccat aagtatcgtg
    14821 ggtcggcgcc tctatcattc ttccgtatga aaggaaaagg agccgaatgg ttgggatggt
    14881 ggggcatatt cgtttcattc gtcatttcaa cgtattat
//
LOCUS       DASULI010000397         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_190296, whole
            genome shotgun sequence.
ACCESSION   DASULI010000397 DASULI010000000
VERSION     DASULI010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190296"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /locus_tag="VFX34_08590"
     CDS             <1..1633
                     /locus_tag="VFX34_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEX5565004.1"
                     /translation="VGKMGLEQTYNEELTGTDGKVDYKTDIWGFILPKSEKKIVAAKD
                     GLTIQLTIDKTIQNFVEDAMNQVDEKYSPQKMLVIVANPKTGEIYAMSQRPSFHPSTR
                     EGLTENWLNDAIQNTIEPGSTMKIFTLAAAIEEKKWDPFAEYQSGQYTIYDKTIRDVN
                     RTGWGRISFLEGFQRSSNVSMAYLLERMGDRTFIEYLRGFGFGEKVGIDLPNEVPGII
                     LDINPSERLTTSYGQGSTVTPIQMIQAATAIANDGKMMKPFVIDKIVDPNTGEVVEDH
                     KVEDRKSPISADTAKQVREVLASTVTSDVGTGRKFALSGYSVGGKTGTAEIPRKDGRG
                     YLSGDGNYLYSFLGMAPIDDPQLLTYVLVQQPKLEIGQYGSDPVAELFTSIMESSLKY
                     MNILPEDSQTSSTKSLSNHIGQDAESSIDNLKQQGFSPILIGEGGKVKSQYPEEGTKL
                     SDGSLVLLETEGDTTLPNFSGWSKKMALSFKMLSGLDIRLNGDGYVTEQSLSAGAVIS
                     KDDPVVLQLKSPEDQHTTIEQDEEDGEEVEEIIGG"
     gene            1726..>2669
                     /locus_tag="VFX34_08595"
     CDS             1726..>2669
                     /locus_tag="VFX34_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5565005.1"
                     /translation="MRAVFIIFLISISMVIAKLFHVQIIKHEWLKARAEENWDREIPF
                     GAIRGEIQDRNGQLIVGNRLAPTLYFMPSQNKEPEKAAALLADILQVDKEKLLKKMTK
                     KTYMNKLAPEGKNITQEQATEISKLQIDGLYTGVDFVRNYPYGELLSRLIGFTGFDGT
                     GLAGIEYAYDEILQGTGDKIRLYTDAKAIPLPHVDDGFQTGENGATLELTIDIEMQKV
                     MERELTQAMEKLDAAQAIGIIMNPKTGEILSLVSMPTFHPAKYQETDQSIYNRNLPVW
                     MTFEPGSTFKIVTLAAGLEEKVIDLHHDYFNDPGYTKIA"
BASE COUNT          802 a          548 c          690 g          629 t
ORIGIN      
        1 tgtcggtaaa atgggtctgg aacagactta taacgaggag ctgaccggga cggacggaaa
       61 ggtagattat aagactgata tatggggatt catcttgccc aaatcggaga agaaaatcgt
      121 tgctgccaaa gatggattga cgatccaatt gacgatcgac aagacaatcc agaatttcgt
      181 cgaagatgct atgaatcaag tggatgaaaa atattctcct caaaaaatgc tggtcatcgt
      241 cgccaacccg aagaccgggg aaatctatgc gatgagccag cgcccttcat tccatccttc
      301 cacccgtgaa ggattgacgg aaaactggct gaacgacgcc attcaaaaca caattgagcc
      361 gggttccacg atgaagatct ttacattggc ggcggcaatc gaggaaaaga aatgggatcc
      421 ttttgctgaa taccaatcgg gacaatatac gatttatgac aagacgatcc gtgacgttaa
      481 tcggacaggc tggggaagga tcagcttcct ggaaggattc cagcgctcct cgaacgtatc
      541 gatggcgtac ctgttggaaa ggatgggcga ccggacattc attgaatact tacgtgggtt
      601 cggatttggc gaaaaggtcg gaatcgattt gcctaatgaa gttccaggca ttattttgga
      661 tatcaatccg agtgagcggc tgacgacttc atatggacaa ggatccactg tcaccccaat
      721 ccaaatgata caggcagcaa cagcgattgc aaatgacggc aaaatgatga agccatttgt
      781 catcgataaa atagtcgatc cgaatactgg tgaagtggtg gaggatcata aagtggaaga
      841 tcggaaatcc ccaatatcgg cggacactgc caagcaagta cgtgaggtgt tggcttctac
      901 agtcacatca gacgtaggaa ctggccggaa attcgccctt tcgggatatt cggtcggcgg
      961 aaagacagga acagcggaaa ttccaagaaa ggatggaagg ggatatttgt ccggtgacgg
     1021 taactatctt tattctttcc tcgggatggc tcctatcgac gatccccagt tattgacgta
     1081 tgtgcttgtc cagcagccga aacttgagat aggccaatat ggatcggatc cggttgcaga
     1141 gttattcact tccataatgg aaagcagctt gaaatatatg aatatactac ctgaggatag
     1201 tcaaacatcc tcaacgaagt cactctcgaa ccatattggg caagacgcgg aatcctcgat
     1261 tgacaatctt aaacaacaag gattttcacc gatattgatc ggggagggcg gaaaggtgaa
     1321 aagccaatat ccggaagagg gcacaaaatt gagtgatggg tctcttgtgc tccttgaaac
     1381 ggaaggggat acaactcttc caaatttttc cgggtggtcg aaaaaaatgg ccttatcatt
     1441 taaaatgtta tctgggcttg atatccggtt gaacggtgat ggatatgtga ccgagcaaag
     1501 tctttccgca ggtgccgtca tcagtaaaga cgatcctgta gtgcttcagc tgaaatcacc
     1561 cgaagatcag catacgacga tcgaacagga tgaagaggat ggtgaggaag tggaagagat
     1621 aattggtgga tgatgtatag ctgtccccgc ccattcatag gttgatagaa aaacgaaagg
     1681 tgtggggctc tgcgtaaata cgccgatttg caatccaaga agagattgcg ggcagttttc
     1741 atcattttcc tcatttccat ttccatggtt atagcaaagc tcttccacgt tcaaatcatt
     1801 aagcatgaat ggttgaaagc gcgcgcggaa gagaattggg atcgggaaat cccttttgga
     1861 gccatcaggg gggagatcca agaccgcaac ggtcagctga ttgttggcaa ccggcttgct
     1921 ccgacgcttt atttcatgcc ttcccaaaat aaggaacccg agaaggcggc agcgctgcta
     1981 gcggatattt tgcaagttga taaggaaaag ctccttaaga aaatgacaaa gaaaacatat
     2041 atgaacaagt tggcgcctga agggaaaaac atcacgcagg aacaggcgac ggaaatttca
     2101 aaactgcaaa tcgatggctt gtatactggc gttgactttg tcaggaatta tccatacggg
     2161 gaacttcttt cgagattgat cggtttcacg ggattcgatg gaacaggcct tgcgggcatt
     2221 gaatatgcat atgatgagat tttgcaaggc acaggagata aaatccgcct ctatacagat
     2281 gcaaaggcga ttccgcttcc gcatgtcgat gatggatttc agacggggga aaatggtgcc
     2341 actttagaac tgacaatcga tattgaaatg cagaaagtca tggaacgcga attgacgcaa
     2401 gcgatggaaa aactcgatgc cgcccaagcg atcgggatta tcatgaatcc gaaaacgggc
     2461 gaaatactat cgttggtttc catgcctact ttccatccag caaaatatca ggaaacagat
     2521 cagtcaatct ataatcggaa tttgccggtt tggatgacgt ttgaaccggg atcgacattc
     2581 aagattgtaa cactcgcggc gggtcttgaa gagaaagtga ttgacctcca tcatgattat
     2641 ttcaatgacc ccggctacac gaagattgc
//
LOCUS       DASULI010000398         5132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1530358,
            whole genome shotgun sequence.
ACCESSION   DASULI010000398 DASULI010000000
VERSION     DASULI010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530358"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VFX34_08600"
     CDS             complement(<1..1009)
                     /locus_tag="VFX34_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HEX5565006.1"
                     /translation="MQKHPDFEFELQRLDYTKHYMQKLLEESMRDVQSSQDEIRHAMA
                     DLDYLDSSLSYINILTNTRFFEMARSQKEGLEAIQQKPYFARIHFQTEGEKDEFLYIG
                     KTSVFHQETQEPIIVDWRSPVANVYYDGRLGDLTYKVRDEEISGHLFSKRQYKIEEGE
                     LLDILDIDLTTNDELLQEALAGKADVRLTEIVSTIQAEQNEIIRANLKQPIIVQGAAG
                     SGKTTIALHRISYFLYTMGTDFPSEKLMILAPSRLFMEYIGDVLPELGVGRICQTTFA
                     EYVLDATGLKLKLADPNKKLELLTAADSVDEEQLFIARMKGTLRYREIMQKYVDKLEQ
                     ET"
     gene            complement(1189..2877)
                     /locus_tag="VFX34_08605"
     CDS             complement(1189..2877)
                     /locus_tag="VFX34_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5565007.1"
                     /translation="MRYSVRKKLWTGFLSLVILMIIAGVFMYASIPRISKEYNFLIDD
                     RMEKVILLEQLSTNQNELSNDLRGYLLYKSISYLKNRQELMESFEDKVNQLDAAFQSE
                     ESQVLLDELIDAADEYGKLTDSAITSFNNARDDEALTYASQAEAYQTVITAKANKLID
                     IQRAEMLETKAELEGFLKSTGIFLIGIIALVTILSIIIAFVISRMITKPVGKMTDALK
                     QLADGNFAIDPLQIKNRDEIGDMATAFNGMALDLRNVVTNTRDSAYQLAAQAEQLTAS
                     SEESLAASEMVAEIAEKNLLASDMQVNLVNESSAAMGEMASGIQQITEDNEAMLSSSN
                     EVSRLVGEGADLMENVNNQMSAIAASIRESADVITDLAVHSEKIRNVTGLITGIAEQT
                     NLLALNAAIEAARAGEQGKGFAVVANEVRNLAEQSKESAEEIGRMIDAVIQNVSMAVD
                     STEAGTERVNEGVGITEQTHAVFNRIEEASKDVSDKVGTVSAAIEQIRSMTNRVAESS
                     NKVHELAVQASSEAQSTSAATEQQLASNQEIASSSQLLSEVAEKLQNDMGRFTV"
     gene            complement(3078..3761)
                     /locus_tag="VFX34_08610"
     CDS             complement(3078..3761)
                     /locus_tag="VFX34_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016083709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565008.1"
                     /translation="MKKDLMLRSGQEVTIRSLALDDLGTIMELQEKVIASLTVTSFLQ
                     PLSVEEFRFILNGNGSMIGAFHDNRLIAFRAMLEPEIDDHHLGKDADLPDSALPSVLY
                     SEVTNVDPEYRGNSLQRILGEFLMQNIDRARHRYVFATVAPFNIASLKDKFALGMHII
                     SLQKKYGNLLRYTLMKDLQANEQTGTLTITADMSDSMKQQSLLGDGWIGVGLEEVDGT
                     WKVKFQRNE"
     gene            complement(3758..4870)
                     /locus_tag="VFX34_08615"
     CDS             complement(3758..4870)
                     /locus_tag="VFX34_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX5565009.1"
                     /translation="MKIKEIEIYAIHLPLHEPFIISYARYDTMPSIIVKLMTDTGHIG
                     YGEAVADEHVTGESWESTYAVLKNTLAPKLIGMNPAHMEKIHERMDAEVYGVPSAKAA
                     LDIACYDAVGKALGVPVYDLLGGRYHDEFPITHVLSIGTPLHMADEAEQRVKEGYRSL
                     KMKVGTDVSEDVKRIQAVRERVGESIAIRIDVNQGWVNSSTTLQALRKLEACSLDWLE
                     QPVKADDIDAMVEIKSKTSVPVMIDEGLRGIREMREIIAKRAADKVNIKLMKCGGIYP
                     AMKLAHMAEMAGIECQVGSMVESSVGSAAGFHVAFSKKIMTSVELTGPLKFSEDIGNL
                     TYNVPFIRLTDKPGLGVDIDEAVLNKLTVFSEKVVG"
     gene            complement(5001..>5132)
                     /locus_tag="VFX34_08620"
     CDS             complement(5001..>5132)
                     /locus_tag="VFX34_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5565010.1"
                     /translation="KTVFDRLKQGGSVVMDLQETFWSKSYGSLKDQFGIEWQISHEE"
BASE COUNT         1207 a         1306 c         1118 g         1501 t
ORIGIN      
        1 ctgtttcctg ctccagctta tccacatact tttgcatgat ttcccggtag cggagcgtac
       61 ctttcattcg tgcaatgaaa agctgttctt catccacaga atcagctgcc gtcaagagct
      121 ccagcttttt attcggatca gccaacttca gtttcaaacc agtcgcgtcc agtacgtatt
      181 cggcgaatgt cgtttggcaa attcgtccta cccccagttc ggggagaacg tctcctatat
      241 attccatgaa cagtctactt ggtgccaaga tcatcagttt ctccgaaggg aaatcggtgc
      301 ccatcgtgta aaggaaatag gagattcgat gaagggcgat cgtcgtcttc ccacttccgg
      361 cagcgccttg aacgatgatc ggctgtttca ggttggcccg aatgatttcg ttctgctccg
      421 cttggattgt cgaaacgatt tcggtcagcc ggacatccgc tttccccgcc aatgcttcct
      481 gaagcaattc atcattcgtg gtcaaatcaa tatcaagaat atcaagcaat tctccttctt
      541 cgattttata ctgccgcttc gaaaagagat ggccgctgat ttcttcgtcc cgcactttat
      601 acgtcaagtc tcccaatctg ccgtcatagt acacattcgc gacaggcgaa cgccaatcga
      661 cgatgatcgg ttcttgggtt tcctgatgga agacggacgt ttttccgata taaaggaatt
      721 catccttctc tccctccgtc tggaaatgga tgcgggcgaa atacggtttt tgttgtattg
      781 cttcgagccc ttccttctgg gagcgggcca tttcaaaaaa tcgggtgttc gtcaaaatgt
      841 tgatatagct caaactggaa tccaaatagt ccaagtcggc catagcatga cggatttcat
      901 cttgcgagga ttgcacgtca cgcatcgact cttccaggag cttctgcatg tagtgcttcg
      961 tgtagtcaag gcgctgtaat tcaaactcaa aatcgggatg tttctgcatg gacagtcctc
     1021 cgttttgcgt attttgcgtc ctgctgtttt ggtgagatgt tggggttgaa atgaaacaga
     1081 aattgattgt accattatgg gatgttttag gcaatatgat ttattcagaa tcattgtaat
     1141 tagtgattta aaaaagcccc acgccatcta cttggcgtgg agaccttatc agactgtaaa
     1201 tcgtcccatg tcgttttgca gtttttccgc cacttcggaa agcaattggc tgcttgaggc
     1261 gatttcctga ttggatgcca attgctgctc tgtcgctgcg ctcgtagact gtgcttcgga
     1321 ggatgcttgg acggccaatt catgtacttt gtttgaactc tccgccaccc ggtttgtcat
     1381 cgaacggatc tgttcgatag cagcggaaac tgttccgact ttgtcgctga catccttcga
     1441 agcttcttca atccgattga atacagcatg ggtctgctca gtgatgccca ccccttcatt
     1501 cacacgttcc gtcccggctt ccgtgctgtc cactgccatt gaaacgtttt gaatgacggc
     1561 gtcaatcatc cggccaatct cttctgctga ctccttcgat tgctcggcga gattccgcac
     1621 ttcattggca acgacagcaa agccttttcc ttgctcccct gcccgcgcag cttcaatcgc
     1681 ggcattcaat gccagcaaat tcgtctgctc agcaatacct gtaatgagtc cagtcacatt
     1741 ccggattttt tccgaatgaa cggccaaatc ggttatgacg tccgcggatt cgcgaatgga
     1801 agcggctatg gcggacatct gattgttcac gttttccatg agatccgcgc cttcaccgac
     1861 aaggcgggac acttcattgg atgacgatag catcgcttca ttatcttccg tgatttgctg
     1921 gatgccgctc gccatttcgc ccattgcggc cgaagattca ttcacgaggt tcacttgcat
     1981 atcactcgcc aataaattct tctccgcaat ctcagcaacc atttcggaag cggccagact
     2041 ctcctctgaa cttgccgtca attgctcagc ctgcgctgcc aattggtagg cggaatcgcg
     2101 tgtattcgta acgacatttc gcagatccaa cgccattcca ttaaatgcag tcgccatatc
     2161 accgatttca tctctgtttt taatttgcaa cggatcgatt gcaaagttcc catcagcaag
     2221 ttgtttcaaa gcatccgtca ttttgccgac aggttttgtt atcattctgc tgatgacaaa
     2281 ggcgatgatg atgctcaaaa tcgtaactag cgcaatgatt ccaatgagga agatgcctgt
     2341 cgactttaaa aacccttcaa gctccgcttt cgtttcaagc atttcagcac gttggatgtc
     2401 aatcagctta ttcgctttag ctgtaatgac cgtctggtaa gcttccgctt ggctggcata
     2461 tgttaacgct tcgtcatctc ttgcattatt gaaggaggtg atggcggaat cggtgagctt
     2521 gccatattcg tcggcagcat caatcaattc atccaaaagc acctggcttt cctccgattg
     2581 aaaagctgca tcgagttgat tcactttgtc ttcaaatgat tccatcagtt cctgtcgatt
     2641 cttcaaataa ctgatacttt tatataaaag atagccacgt aaatcattcg aaagctcgtt
     2701 ttggttggtt gaaagctgtt caagtaaaat gactttttcc attcgatcgt cgatcaggaa
     2761 gttgtattct ttacttatcc taggaatgga agcgtacatg aagacaccgg caatgatcat
     2821 caaaatgact aaagataaaa aacccgtcca caactttttc cgtactgaat atcgcatttt
     2881 tttcctccta taatcgtaac caaccctcat tataaactac tatctatcag gcgtatagac
     2941 ataggtagat agattgtcga aaacagtcgg aagtaccctg ggtgggcagt tcattcatta
     3001 caacaggaga atgatagtcc tttctaacta aagaaaccgt acaaagtgga ttcttccact
     3061 tcatacggtt ttcctgttta ttcatttctt tggaatttca ctttccacgt cccgtcgacc
     3121 tcttccaggc caactccgat ccagccatct cccaataagg attgctgttt catactatcc
     3181 gacatgtccg ccgtgatggt gagcgtacct gtctgctcat tggcttgcaa atctttcatc
     3241 aacgtgtacc gcaaaagatt tccatatttc ttttgcaatg aaatgatatg catgcctaag
     3301 gcgaatttgt cctttaggct tgcaatattg aacggagcga ctgttgcaaa tacataacga
     3361 tgacgagccc tatcgatatt ctgcatcaaa aattcgccga gaatccgctg taagctgttc
     3421 cctctatatt cgggatccac atttgtcact tcagagtata aaacggatgg caatgccgaa
     3481 tcaggcaagt cggcatcttt ccctaaatga tgatcgtcaa tttccggttc gagcatcgcc
     3541 cggaatgcga taagacgatt gtcatgaaaa gctccgatca ttgagccgtt gccgtttagg
     3601 ataaagcgga attcttccac ggataacggc tgcagaaaag atgtgaccgt caaggaggca
     3661 atcacttttt cctgcaattc catgattgtc ccgaggtcgt cgagggccaa gcttcgaatc
     3721 gtcacttctt ggccgcttct taacatcaag tcttttttca tccgactacc ttctctgaga
     3781 agacggtcaa tttattcaag acagcttcat caatatcgac gccgagaccg ggtttatccg
     3841 tcagacggat gaatggaacg ttgtacgtta agtttccgat gtcctcactg aattttaaag
     3901 gccccgtcaa ttcgacgctt gtcatgatct tcttggagaa ggcgacatgg aatcccgccg
     3961 ctgaaccgac ggacgattcg accatcgagc cgacctggca ttcgatgccc gccatttccg
     4021 ccatatgggc aagcttcatc gccggataga tgccgccgca tttcatcagt ttgatgttca
     4081 ctttatccgc ggcgcgcttc gcgatgattt ccctcatctc acggattccg cgcaagcctt
     4141 cgtcaatcat gacagggacg gatgttttcg atttgatttc caccatggca tcgatgtcat
     4201 ccgcttttac cggctgttcc agccaatcga gtgaacaggc ttcaagtttt ctcaaggctt
     4261 gcaacgtcgt ggaactgttc acccatcctt gattcacatc aatgcggatg gcgatcgact
     4321 ccccgacacg ttcgcggaca gcctggatcc gtttcacatc ttcggatacg tctgtgccga
     4381 ctttcatttt caatgaacga tagccttcct tgacgcgctg ttctgcttcg tccgccatat
     4441 gtagaggtgt gccgatgctc agaacgtggg tgatcgggaa ttcatcatgg tatcttccgc
     4501 ccaataaatc gtaaacggga acgcccaatg cttttccgac tgcgtcatag caggcgatgt
     4561 ccagtgcagc tttcgccgat ggcacaccgt agacttcagc gtccatgcgc tcgtggatct
     4621 tttccatgtg ggcaggattc atgccgatca gcttcggcgc caacgtattt ttcaggacgg
     4681 catatgtact ttcccagctt tcaccggtga cgtgctcgtc cgccaccgct tccccgtatc
     4741 cgatatgccc ggtgtccgtc atcaatttga cgatgatcga aggcatcgta tcatatcttg
     4801 cgtagctgat gatgaatggt tcatgtaaag gcaaatggat cgcatagatt tcaatttctt
     4861 ttattttcaa gatatgtctt cctttcgcaa tggcaaatgt ctttcattat tgtagcattt
     4921 ccggaattgt ggaggaagat tggaccttga tgaaataaca aaaacgacga gtgcaaagac
     4981 ccgtcgttcc gcgtacatta tcattcttcg tggctgatct gccattcgat gccgaattga
     5041 tccttcagcg agccgtagct tttgctccag aatgtctcct gcagatccat tacgacggat
     5101 ccgccttgct tcagtctgtc aaaaacggtt tt
//
LOCUS       DASULI010000399         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1681430,
            whole genome shotgun sequence.
ACCESSION   DASULI010000399 DASULI010000000
VERSION     DASULI010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681430"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFX34_08625"
     CDS             <1..366
                     /locus_tag="VFX34_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX5565011.1"
                     /translation="IGYIIFTALLNFLITSGSAKWALEAPVFVPLFMNLGYHPAFTQV
                     AYRVADSSTNIITPLNAYFVVILSFMREYDKKAGIGTLISLMIPYSIAFLSVWIILIL
                     VFFYAGIPFGPGVTPFYTH"
     gene            454..1776
                     /locus_tag="VFX34_08630"
     CDS             454..1776
                     /locus_tag="VFX34_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5565012.1"
                     /translation="METKQAIEVLQQQLQKWRRDFHQNPEIGFMEMRTASIVARYLED
                     LGYEVRAGEEVMKREARMGIPDKAALDGHFEWARQNGADEKWLERLKDGMTGVVGVLD
                     TGKPGPVIAFRVDMDALEIQEELSPGHFPYDEGFASVHKKRMHACGHDGHTAIGMGLA
                     KLLMEEKDALNGVVKLIFQPAEEGTRGAKSMTEAGVVDDVDYFIATHIGTGVPSRHIV
                     AGNAGFLATTKMDVLIKGKAAHAGAEPNEGKNALLAASTAVLGLYGIPRHAGGASRIN
                     VGVLEAGTGRNIIASEAYLQIETRGETSEVNDYMRAQALSVLHGAAAMHQTEVDIQIV
                     GEAVSSTPSDELVNIISEVAASSPYVDQVIPMSTQSAGSEDATYFMERVKDRGGLATY
                     TIVGTELAAGHHHERFDYKEDVLLHGVDLLFRTAKVLAAVNDEKECQA"
     gene            1773..3206
                     /locus_tag="VFX34_08635"
     CDS             1773..3206
                     /locus_tag="VFX34_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX5565013.1"
                     /translation="MTDLQKIVQLVEEKKEKWVDVSRKIWGFAETRFEEVQSAALLCE
                     VLEAEGFQVEKGLADMPTAFVGSYGSGGPVIAFLGEYDALSGLSQEGGTPISAPIEQG
                     GNGHGCGHNLLGTGSLAAAVALKDLMVQEGLQGTIRFYGCPAEEGGSGKTYMTRAGIF
                     DDIDFALCWHPWSTNEIFTGNSLANYQIYYRFKGKSAHAAAAPHLGRSALDAVELMNV
                     GVNYLREHIIPEARVHYAITNSGGFSPNVVQAEAEVLYLIRAPKTPQVQEIYERVCNI
                     AKGAALMTGTEVEIVFDKACSNLIPNKTIETKMHEVFTEIGLPQYTEEELAFAQQLQA
                     TLSRADIENDMGKMPKEVAAQLKGKAMADVIHPYNPDAEPSIFGSTDVGDVSWVTPTA
                     QLFGACWVLGTPAHTWQVVSIGDTSIAQKGMLLAGKVMAGTALEMLKQPELIEKSKKE
                     LKERLGDDTYKCPIPAHIMPASNEKKQ"
BASE COUNT          951 a          710 c          915 g          812 t
ORIGIN      
        1 attggctaca tcattttcac agctttgctg aatttcttaa ttacatccgg gtcagcgaaa
       61 tgggcattgg aagctccggt attcgtgccg ctgttcatga atcttggata ccatccagca
      121 tttacacagg tcgcctatcg ggttgctgat tcatcaacga atatcattac gccgttaaat
      181 gcgtatttcg tggtgatttt atcgttcatg cgcgagtatg acaaaaaagc gggcattgga
      241 acattgatat ccttaatgat tccttattcc atagcgttcc taagtgtgtg gatcattctt
      301 attcttgtgt tcttctatgc tggcatccca tttgggccag gcgtaacgcc attctataca
      361 cattaatcat gaatcgacat ggcgtactgt tgcggaaatt ccgtggcgtg tacgccattc
      421 tttaataatg aaactattcg ggggtggact tttatggaga cgaaacaagc tattgaagtg
      481 ctgcaacaac agcttcaaaa atggagacgt gattttcatc aaaatccgga aatcggtttt
      541 atggaaatgc ggaccgcatc gattgtcgcc cgctatttag aagacttggg gtatgaagta
      601 cgcgcaggtg aagaagtgat gaaacgggaa gcaagaatgg gcataccgga caaggctgcg
      661 ttggatggac atttcgaatg ggcaaggcaa aatggtgccg atgaaaaatg gctggaacgc
      721 ctgaaggatg gtatgactgg cgttgtgggc gttctcgata ctggaaaacc cggtccagtt
      781 atagcgtttc gggtagatat ggatgcgctt gaaattcagg aagaattatc acccggacat
      841 tttccatatg acgaaggatt tgcttccgta cataaaaaaa ggatgcatgc atgtggtcat
      901 gacgggcata cggccatcgg gatggggcta gccaaactgt tgatggaaga gaaagacgcg
      961 ctcaacggag tcgtgaaatt aattttccaa cctgcggaag aagggacacg cggtgcaaaa
     1021 tcgatgaccg aagcaggtgt cgtggatgat gtggactact ttatcgcaac acatataggg
     1081 acaggggttc catcccgtca tatcgttgca gggaatgccg gtttccttgc aacgacgaaa
     1141 atggatgtcc ttattaaagg aaaagcggct cacgccggtg cagaaccgaa tgaagggaaa
     1201 aatgcattgc tggccgcttc gaccgccgta ttgggattat acggaatccc gcgtcacgcg
     1261 ggcggtgctt cccgcatcaa tgtaggcgtt ctggaagctg gaaccgggcg caatattatc
     1321 gcgtcggaag catacttaca aattgaaacg cgcggggaaa catcggaagt gaatgattac
     1381 atgcgcgcgc aggctttatc ggttttacat ggggcagctg ctatgcacca aacagaagtg
     1441 gacattcaaa tcgtagggga agccgtgagc agtacgccaa gcgatgaatt agtgaacatc
     1501 atttcagaag tggcggcgtc ttcaccgtat gttgaccaag tcatcccaat gagcactcaa
     1561 tccgccggtt ccgaagacgc cacttatttc atggaaagag tgaaagaccg cggcggcctt
     1621 gcaacgtata caatcgttgg aactgagttg gcggcggggc atcaccatga acggttcgac
     1681 tataaggagg atgtcctcct gcatggtgtt gatctattgt tccgcacggc gaaagtgctt
     1741 gcagcagtaa atgatgaaaa ggagtgtcaa gcatgaccga tcttcagaaa atcgttcaat
     1801 tagtagaaga gaagaaagag aaatgggtag atgtcagcag aaagatttgg ggatttgcgg
     1861 aaacgcgatt tgaggaagtc caatcagctg cactgctttg tgaggtgctt gaggcagaag
     1921 ggttccaagt ggaaaaagga cttgcagaca tgcccacagc tttcgtaggc agttatggtt
     1981 ccggcggtcc tgtcatcgct tttctaggcg agtatgacgc gctttcagga ttaagccaag
     2041 aaggaggaac gccgatttca gcgccgattg agcagggcgg aaacggtcat ggctgcggtc
     2101 acaatttgct gggaacgggt tctttggccg cggctgttgc gctcaaggac ctgatggtgc
     2161 aagaaggctt gcaagggacg attcgttttt atggatgtcc agcggaagaa ggcggttccg
     2221 gaaaaacgta tatgacacgt gcgggcatat ttgatgatat cgattttgca ctctgttggc
     2281 atccgtggtc gacaaacgaa attttcacgg ggaactctct ggcgaactat caaatctact
     2341 atcgcttcaa agggaaaagc gcacacgcgg cggcggctcc gcatcttggc agaagcgcat
     2401 tggatgcggt tgaattgatg aacgtcggtg tgaactactt gcgtgagcat attatccctg
     2461 aagcaagggt tcactacgca atcaccaatt ccggcggctt ctcgccgaac gtagtgcaag
     2521 cggaggcgga agtgctttac ttgatccgtg cacctaaaac tccgcaagtt caagagattt
     2581 acgagcgggt atgcaatatt gcaaaaggcg cagcattaat gacaggaaca gaagtggaaa
     2641 tcgtttttga taaagcttgc tcgaatttga tcccgaataa gacgatcgaa acgaaaatgc
     2701 atgaagtgtt tactgaaatc ggcttgccac aatatactga agaagagctt gcgtttgcgc
     2761 aacagcttca agcgacgttg tcacgtgcag acattgaaaa cgatatgggg aaaatgccga
     2821 aagaagtggc ggcgcaattg aaaggaaaag caatggcgga cgtcattcat ccatacaatc
     2881 cagatgcaga accttctatt ttcggttcaa ccgacgttgg agatgtaagc tgggtaacgc
     2941 caaccgcaca acttttcggt gcgtgctggg ttctcggcac accagcccat acatggcaag
     3001 tcgtctccat cggggacaca tcaatcgcgc aaaaaggaat gctgctggca ggtaaagtaa
     3061 tggccggaac ggcactcgaa atgctgaaac aaccagagct gatcgagaaa agcaagaaag
     3121 aactaaaaga acgccttggc gacgacactt acaaatgccc aattccagcc catatcatgc
     3181 cagcaagcaa tgaaaaaaaa caatagaacc ggaaattgtg tcggtgcctg tgaatgacac
     3241 atttatgaca attcaataaa aggtatgaaa atacgacggg aagctaaccg tgtcttgaca
     3301 tggcttgctt cctgtttctt tatatgcaat tgtagtgtat taactgaatt gtgtcggtgc
     3361 ctgtgcatga cacatttatg acaattca
//
LOCUS       DASULI010000400         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1802646,
            whole genome shotgun sequence.
ACCESSION   DASULI010000400 DASULI010000000
VERSION     DASULI010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802646"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1406)
                     /locus_tag="VFX34_08640"
     CDS             complement(192..1406)
                     /locus_tag="VFX34_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010532092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5565014.1"
                     /translation="MTGRGMMQHDGYTLRNLQFWKIVLGLGFASVFIFAAMYSMQPLL
                     PLFTKEFGISVSYASMAMSMTTLGLIVGLIVLGFFSDRNGRNLYIYLSLIGSVVPFLI
                     IANTDSFLFIVILRFVQGFALAGVPAAALAYISEEVHKNFTNIATALYISFNSLGGMI
                     GRFMTGFIAEQASWQMSLYILSIFGTVLFILLLMTLPKSRNFVPSDTTFAKDIEGIVF
                     HLKNPALLIVFGLGIVLQLSFTGVWTYLPFHLIEAPYSLSLETISYLYLAYGFGVVGA
                     PLAGWLSGKFGLRNIRLAGISLLALGIGMTALAPLAFIIIGLCIVCLGFFTAHSLTAA
                     SVSKEATHHKGSAASLYLVSYYIGVAMGSTLLSPLYETVGWEGLIAFTAILPIVYVGM
                     IRFRQRNKNRAA"
     gene            complement(1524..1868)
                     /locus_tag="VFX34_08645"
     CDS             complement(1524..1868)
                     /locus_tag="VFX34_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565015.1"
                     /translation="MSRPQVEFINYQSIKPEVIEGVVKGLSQRVISQDPESGDLARIL
                     EFEPGTDTSRNGVQVHDYWEEIYIIDGSVIDLTLNKEFTKGMVASRPPGMEHGPWKSF
                     HGCTMFEVRYYK"
BASE COUNT          633 a          509 c          431 g          529 t
ORIGIN      
        1 cgtcgtattc ggttccggct atttacggat tcccgacatg gccaaagctg gatttgcact
       61 taacatcatt ggaattgtcc tcgtgacagc agccatctat ttcttgctgc cgcttttctg
      121 gggaatcgat ttgacgacga ttcccgagtc gtttaaatga atgaattgaa aaaacagggc
      181 tagcctcata tttaggctgc cctgttttta ttcctttgtc taaacctgat catcccaaca
      241 tatacaattg gtagaattgc tgtaaaagca atgagacctt cccagccaac cgtttcatac
      301 agcggactaa gcaatgtact ccccatcgcc acgccgatgt aataggaaac caagtaaagg
      361 cttgccgcac tgcccttatg atgagtcgct tccttgctga ctgacgctgc cgtcaaggag
      421 tgtgccgtga agaaccctaa acagacgata catagaccga tgatgatgaa cgcgagcggt
      481 gcaagtgctg tcatgccgat gcccaatgca agcaaactaa tccctgccag ccttatattt
      541 cggaggccga acttccctga tagccaccct gcgagtgggg ccccgacgac tccaaaccca
      601 tatgccaaat ataagtagga gattgtttcg agtgaaagtg aatacggtgc ttcaatgagg
      661 tggaatggta aatacgtcca cacaccggta aatgacaatt gaaggacgat tcccaggccg
      721 aatacgatga gcaatgcggg atttttcaaa tgaaatacga tgccctcaat atcttttgca
      781 aaggtcgtat cactcggcac gaaattccgc gatttcggca aagtcatcaa caaaagaatg
      841 aataacaccg tcccgaaaat cgaaagtatg tataaagaca tttgccacga agcctgctcg
      901 gcgatgaatc ctgtcatgaa tcggccgatc atgcctccca aactgttaaa agagatgtag
      961 agagctgttg cgatgtttgt aaaattctta tgcacttcct cgcttatata agcaagcgct
     1021 gcggccggca cgccagcgag cgcaaaccct tgcacgaagc gcagaatgac gatgaacagg
     1081 aaggaatccg tatttgcgat aatcagaaac ggaacaaccg atccgatcaa ggaaagatat
     1141 atgtatagat tccttccgtt ccggtccgaa aagaaaccga ggacaatgag cccgacaatg
     1201 agcccgagtg tcgtcattga cattgccata cttgcatacg agaccgaaat accgaattct
     1261 tttgtgaata aaggtaggag cggctgcatt gaatacatcg ctgcaaagat gaaaacagaa
     1321 gcgaacccca agcctaacac gattttccaa aattgaagat ttctcaacgt atagccgtca
     1381 tgttgcatca tcccacgtcc agtcatcagc ctattttctt ttattgtacc atttatataa
     1441 aatgaaaagc ggcaccggat agatgcataa atgagtcgga tagccatacc gcccctacct
     1501 ggcccactac aagacccctg ttactattta taataacgga cctcaaacat tgtacaaccg
     1561 tgaaaggact tccaaggtcc atgctccatg ccaggtggcc gggatgctac cattccttta
     1621 gtgaattcct tatttaacgt caaatcaatg actgagccgt caatgatgta tatttcttcc
     1681 caataatcat gaacttgaac gccattccga gatgtatcgg tgccgggttc gaattctaga
     1741 atgcgtgcaa gatcacccga ctccggatcc tgtgaaataa ccctttgtga taatcctttt
     1801 actactcctt caatcacttc cggcttgatg ctttggtagt ttataaattc aacctgtggc
     1861 cttgacacag taacccctcc atttacattc catgattaaa aaatcgggcg tcttctattt
     1921 actatgacaa ccatgggtaa tcatatagaa gacgccctat caatgactct caaagaaaca
     1981 actgacaatc atccaccgct taacaaattc accgggaata atattacatc cggtaaataa
     2041 gtaataacga gtaagacgat aataagaata ataatttgcg gcaccaagta cttgaacatt
     2101 tt
//
LOCUS       DASULI010000401         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_504763, whole
            genome shotgun sequence.
ACCESSION   DASULI010000401 DASULI010000000
VERSION     DASULI010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504763"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VFX34_08650"
     CDS             complement(<1..1208)
                     /locus_tag="VFX34_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5565016.1"
                     /translation="MSNARAELVRQSFVTHVDENRLMDCMRCGFCLPACPTYIHSYYD
                     ETQSPRGRIALMKAVRDGDVEWDGSIEEAFDLCLGCRACEPACPAGVQYGTLIEETRE
                     AVLSVKSQSFKEKAIRKGAFDHLFADQRKMKTAVKLVSFYQKSGLQKVVRKVGFLELF
                     PPFMKEMERSLPDPPKRRREQEQPTEKKTTVAFFAGCLMDTMFQETNRNTIDLLNRLG
                     CEVVIPKNQQCCGALQGHSGEMDRARRNAKANLEVFDLDGIDYIVNNAGGCGAFLSEY
                     DRLFEEDPTLLELARRFVGKQIDISSLLVKLGVVDELMKQPTAEATEIVTYQDSCHLR
                     NVNGVKDEPREILKAVKGSLFVEMPGAHLCCGSAGVYNLLQPEMASRILGSKMEEVKR
                     VRATTIITTNP"
     gene            complement(1201..2613)
                     /locus_tag="VFX34_08655"
     CDS             complement(1201..2613)
                     /locus_tag="VFX34_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5565017.1"
                     /translation="MDEQVIERLIAITGSAHARTSIAQKLAYSYDATANFQALPDIVL
                     SPASIGEIQEIVKVCAEYGVPIVSRGSGTNLAAGTTPTSGGVVVLFNRMNRILELDEE
                     NLTVTVQPGVITQTINERVAEKGLFYPPDPSSMSISTIGGNVSENSGGLRGLKYGVTR
                     DYVKALKIVLPSGELLTTGGKLAKDVAGYDLTSLFVGSEGTLGIIVEITLGLIPAPES
                     KKTLLVFYDTLEQAAQTVSSIIAAKIVPATLEFMDKGTIEAVEEFMKIGLPTNCEAML
                     LIEQDGDPSVVEKDMELVHRLCMAHGATSTQMAQTAEEAAQLSIARRVALSALARKRP
                     TTILEDATVPRSEIAKMVKAVQEIALKYDLLICTFGHAGDGNLHPTILTDVRDEAEMA
                     RVEMAFGEIFDQAIKLGGTITGEHGVGEMKAPYLEWKIGKAGIDTMKSIKVAIDPNNI
                     MNPGKIFAKSAKKRVVVHHE"
     gene            complement(2758..>3184)
                     /locus_tag="VFX34_08660"
     CDS             complement(2758..>3184)
                     /locus_tag="VFX34_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5565018.1"
                     /translation="IIVAAGIGGKTDYSDLSKSFIQAERASNASKKLKRILFEHELRF
                     ELILQSIGESTREEFIERTVAPLLEDRELLQNLHVWFNENQSMQNTAKKLHIHKNTLS
                     YRLQKVEQLTGLSVSNVHDVLLLYLGIRLLDEMHDNGIR"
BASE COUNT          684 a          916 c          733 g          851 t
ORIGIN      
        1 ggatttgttg taatgattgt cgttgcccga acccgtttca cttcttccat tttcgagccg
       61 aggatccgtg atgccatttc tggttgaagt aaattatata caccggcgga accgcagcat
      121 aaatgcgcac cgggcatttc cacaaataga ctgcctttga ccgctttcaa gatttccctt
      181 ggctcgtcct ttacaccgtt gacattccgt aaatggcagg aatcctgata ggtgacgatt
      241 tcagttgctt ccgccgtcgg ttgtttcatc agttcatcga caacgcctag cttcacgagc
      301 aatgaggaaa tatcaatctg ttttccaacg aaccgccgtg ccagctctag cagtgtcgga
      361 tcctcttcga acaaccggtc atactcggac aggaacgctc cgcatcctcc ggcgttgttg
      421 acgatgtagt cgatgccgtc gagatcgaat acttcgagat ttgctttcgc gttccgtctc
      481 gcccggtcca tttcaccgct atgcccttgc aaggcgccgc agcattgctg gttttttgga
      541 atgacgacct cacagccaag cctgttcaac aagtcgatcg tattccggtt cgtctcctgg
      601 aacatcgtat ccatgaggca accggcgaaa aaggcgaccg tcgttttctt ttcggtcggc
      661 tgctcctgtt cccttcgcct tttcggcggg tcgggcagcg acctttccat ctctttcatg
      721 aatggcggga atagctccaa gaaaccgacc ttccgcacga ctttctgcaa acccgatttc
      781 tgatagaacg acacgagctt cactgcggtc ttcatcttcc gttggtcggc gaaaagatgg
      841 tcgaacgccc ctttccggat cgccttctcc ttgaatgact gcgatttgac ggataacacc
      901 gcttctcgcg tctcttcgat aagcgttccg tattgcacac ctgcgggaca tgcaggttca
      961 catgcccggc agcccaggca caggtcgaac gcttcctcga tggagccgtc ccattcgacg
     1021 tcaccgtccc ggactgcttt cataagggcg atcctgcccc gcggcgactg ggtttcatca
     1081 taataggagt ggatgtatgt agggcatgcc ggcagacaga aaccgcagcg catgcaatcc
     1141 atcaacctgt tttcatcgac atgtgtgacg aacgattgcc gtaccagttc agcccgggcg
     1201 ttactcatgg tgcaccacta cccttttctt cgccgacttg gcaaatattt tcccggggtt
     1261 catgatgttg ttcggatcga tcgccacttt gatgcttttc atcgtatcga ttccagcctt
     1321 gccgattttc cattccaaat agggagcttt catttcaccg actccgtgtt cacctgtaat
     1381 cgtgccgccg agtttaatcg cctggtcgaa gatttccccg aacgccattt ccaccctcgc
     1441 catttccgcc tcatcccgca catccgtcaa aatggtcgga tgtaagttgc catctcccgc
     1501 atggccgaat gtgcaaatga gcagatcgta tttcaacgcg atttcctgga cagccttcac
     1561 catcttcgca atttcggacc gcggaacagt cgcatcttca agaattgtcg tcggccgttt
     1621 cctcgcaagc gcggataagg caaccctgcg cgcaatgctc aactgggcgg cctcctctgc
     1681 cgtttgcgcc atctgtgtcg atgttgcgcc gtgcgccatg cacagtctgt ggacgagttc
     1741 catatccttt tcaacgaccg atggatcgcc atcctgttcg atgagcagca tcgcttcgca
     1801 atttgtcggc aagccgattt tcatgaactc ctcaaccgct tcgattgttc ctttgtccat
     1861 gaactcaagt gtcgccggca cgatttttgc cgcaatgatc gacgaaaccg tctgcgccgc
     1921 ctgttcgagc gtgtcgtaga atacgagcaa cgtctttttc gattcaggtg ccgggatgag
     1981 acctaatgtg atttccacga tgatccctag ggtcccttcc gaaccgacga acaagcttgt
     2041 caaatcatag cccgccacgt ctttcgcgag ctttccgccc gtcgtcagca gctcaccgct
     2101 cggcaggacg attttcaacg ctttcacata atcgcgtgtg acgccatact tcaagccccg
     2161 taagccccct gaattctcgc tcacattccc gccgatcgtc gaaatgctca tcgaacttgg
     2221 atccgggggg taaaacaagc ctttttcagc aaccctttca ttgatggtct gcgtaatgac
     2281 ccccggctgg actgtcaccg tcaaattctc ttcatccagc tccaaaatcc ggttcatccg
     2341 gttgaataaa acgactacgc ccccggatgt cggtgtagtt cccgccgcaa gattcgtacc
     2401 ggaaccccgg gagacgatcg ggaccccgta ctccgcacat actttcacaa tctcttggat
     2461 ttctccgatg gaagcaggag aaaggactat atccggcaac gcctggaaat tagcggtcgc
     2521 atcataagaa tacgcgagct tctgcgctat cgacgtcctc gcatgagcgc tacctgtgat
     2581 ggcaatcaat cgttctatca cttgctcatc catgaagcag cctccttcaa atttctaatt
     2641 ctttgtttaa ttctaatagt ccgatgatta ggaagatata ggagcatgca caaccttttg
     2701 gtcatttttc gacatttttt tgtttgcgcg aacaaaaaag cacccgtcga atctaattca
     2761 acggatgcca ttatcatgca tttcgtccag caggcggatt ccaaggtaaa ggaggagaac
     2821 atcatggaca tttgaaacgg atagtcccgt gagttgctcg accttctgta gcctgtacga
     2881 aagcgtgttt ttatggatat gcaatttttt cgccgtgttc tgcatcgatt ggttttcatt
     2941 gaaccagaca tgaagatttt gcagcagttc ccgatcttcc agcagcggag ccacagtccg
     3001 ttcaataaat tcttcgcggg tcgattcccc gatgctttgc aggatcagct cgaagcgaag
     3061 ttcatgttcg aacaggatcc gcttcaactt cttggaggca tttgacgccc tttccgcttg
     3121 gatgaatgac ttcgacaaat cggagtaatc ggtctttcct ccgatccccg cagcgacgat
     3181 gatc
//
LOCUS       DASULI010000402         4480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_421566, whole
            genome shotgun sequence.
ACCESSION   DASULI010000402 DASULI010000000
VERSION     DASULI010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421566"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFX34_08665"
     CDS             <1..333
                     /locus_tag="VFX34_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5565019.1"
                     /translation="ESLKAAEALEKEGHSVEVIDLRTVQTLDIETIIGSVEKTNRAIV
                     VQEAQKQAGIAANVVSEITERAILSLEAPVLRVTAPDTVYPFAQGENTWLPNVNDIIE
                     TAKKVLTF"
     gene            379..1719
                     /locus_tag="VFX34_08670"
     CDS             379..1719
                     /locus_tag="VFX34_08670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5565020.1"
                     /translation="MAYEFRLPDIGEGIHEGEVVKWFVAKGDKINEDDTLLEIQNDKA
                     VVEIPSPVTGTVEEILVEEGKVAVVGDVLIRFDAPGYESHSVEEEGAEETEAQVQATA
                     EAGQEIDKEEVKKDEPEQKQAATSAPAQTGDASSKRVIAMPSVRKFAREQEVDISQVQ
                     GTGKNGRVLKEDIEAFLNGGQQQPAQAAEETQAAPEVDTKETKTAAPVVLEGDFPETR
                     EKMSGIRKAIAKAMVNSKHTAPHVTLLDEIDVTELVAHRKKFKEIAAEKDIKLTYLPY
                     VVKALVSTLREFPQLNTSLDDEKEEIIQKHYYNIGIAADTDRGLLVPVIKHADRKSVF
                     AISQEISQLAEKARDGKLSPAEMRGASCSITNIGSAGGQWFTPIINHPEVAILGIGRI
                     SEKPIVKNGEIVAAPMLALSLVFDHRVIDGVTGQQALNHIKKLLGNPELLLMEA"
     gene            1724..3136
                     /gene="lpdA"
                     /locus_tag="VFX34_08675"
     CDS             1724..3136
                     /gene="lpdA"
                     /locus_tag="VFX34_08675"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX5565021.1"
                     /translation="MVVGDFPIEVDTLVVGSGPGGYVAAIRAAQLGQKVTIVEKENLG
                     GVCLNVGCIPSKALISVGHRYASAKDSDDMGITATDVKLDFSKAQAFKDGVVKKLTGG
                     VEGLLKGNKVDIVKGEAYFVDANTAKVMDETSSQTYKFKNAIIATGSRPVEIPSFKYT
                     KRVINSTGALNLEEVPGHLVVIGGGYIGTELGSAYANLGAKVTIIEGADDILAGFEKQ
                     MTQIVKKGLKKKGVEIVVKASAKGVEESDTGVVVTYEAKGEEQKVEADYVLVTVGRRP
                     NTDEIGLEEMGVKFLERGLIDVDKQCRTSIPNIYAIGDIVSGPQLAHKASYEAKVAAE
                     AISGEKSEVDYMAIPAVCFTDPELATVGLNEQQAKDEGYEVVAGKFPFAANGRALALN
                     ATDGFVKLVSRKEDGLLLGAQIVGENASDMIAELGLAIEAGMTLEDIAMTIHAHPTLG
                     EISMEAAEVALGKPIHMITK"
     gene            3443..4285
                     /locus_tag="VFX34_08680"
     CDS             3443..4285
                     /locus_tag="VFX34_08680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5565022.1"
                     /translation="MNKSLLYIPLLSMLLLASCNEEAKPEETDQTAKPAKVEDVTDEV
                     ETDEEQREDGDAQQEEDEVITEPTPLYELNPKTYSIQPIGEANPKVVLLTIDDAPDKR
                     ALDMAKTLKSLEAPAIFFVNGHFITTDEKKAILKEIHDMGFMIGNHTMTHPNLKTLSE
                     EEQREEIIAVNDIIEEVTGERPLFFRAPFGANTDFSRALAIDEGMQLMNWSYGYDFEK
                     EYMTKEKIADIMVNTELLRNGSNLLMHDRDWTADALGDIVNGLRDKGYEFVDPNLIKG
                     LDEE"
     gene            4310..>4480
                     /locus_tag="VFX34_08685"
     CDS             4310..>4480
                     /locus_tag="VFX34_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX5565023.1"
                     /translation="MEWKTRVTELLGIDYPIIQGGLAYLAYADLAAAVSEAGGLGQIT
                     AMSLESPDALRSE"
BASE COUNT         1386 a          907 c         1149 g         1038 t
ORIGIN      
        1 gaaagtttga aagcagcaga agcgcttgag aaagaaggcc actctgtaga ggtcatcgat
       61 ttgcgtacgg ttcagacatt ggatatcgaa acaatcatcg gttctgttga aaagacaaat
      121 cgtgcaattg ttgtccaaga agcgcaaaaa caagcgggaa tagcggcaaa tgtcgtttcg
      181 gaaattactg aacgggcgat cttgagcctg gaagcacctg ttttgcgtgt gacagctcct
      241 gacacggtct atccatttgc ccaaggcgaa aacacttggc tgccaaatgt aaatgatatt
      301 atcgaaacag cgaagaaagt gttgacattc taatttggag acgtaacgac tctctgacaa
      361 tacgaaaggg tgaatcttgt ggcatatgaa ttccgtttac cggatatcgg ggaagggatt
      421 cacgaaggtg aagtcgtaaa atggtttgtt gccaaaggcg ataaaatcaa tgaagatgac
      481 acacttcttg aaatccagaa cgataaagca gttgtggaaa tcccatcacc tgtaacaggt
      541 acagtagaag aaattctcgt tgaagaagga aaagtcgctg tagttggtga cgttttaatc
      601 cgttttgacg ctccaggata tgaaagccat agcgttgaag aagagggcgc agaagagact
      661 gaagcccaag tccaggcaac tgcagaggca ggacaggaaa tcgataaaga agaagtgaag
      721 aaggacgagc ctgaacagaa gcaagcagca acttctgcgc cagctcaaac tggtgatgct
      781 tcatcgaagc gtgtcattgc catgccttca gtacgtaaat tcgcacgcga acaggaagtc
      841 gacatcagtc aagtgcaagg gacagggaaa aatggacgtg tgttgaaaga ggacatcgaa
      901 gccttcctga acggcggaca gcagcaacct gcccaagcgg ctgaagagac acaagctgct
      961 ccagaagtgg acacaaaaga aacgaaaact gctgcgcctg tcgtcttaga aggtgacttc
     1021 ccggaaacac gtgaaaaaat gtcgggaatc cgtaaggcga ttgccaaggc catggtcaat
     1081 tccaaacata ctgcgcctca tgtaacgttg ttggatgaaa tcgatgtgac tgagctcgtg
     1141 gcgcaccgta agaaattcaa ggaaatcgct gctgagaaag atattaagct cacttacctt
     1201 ccgtacgtcg tgaaggcgct tgtatctaca ctgcgtgaat tccctcaatt gaacacatcc
     1261 cttgatgatg aaaaagagga aatcatccag aagcattact acaatatcgg catagcggca
     1321 gatacggacc gtggtttgct cgtaccggtc attaagcacg ctgatcggaa atcggtattt
     1381 gcgatttcac aagaaatcag ccagcttgcc gaaaaagcgc gggatggcaa attgagccct
     1441 gctgaaatga gaggggcatc atgctccatc acgaatatcg gctctgcagg cggccaatgg
     1501 ttcacaccga ttatcaacca tcctgaagtt gcaattctag gaatcggccg tatttccgaa
     1561 aagccgattg tgaaaaatgg tgaaattgtt gccgcaccta tgttagcatt atcattggta
     1621 ttcgatcatc gggtgattga tggtgttaca ggacagcaag ctctgaacca cattaagaaa
     1681 ttgctcggta atccagaact tttattaatg gaggcgtaag caaatggtag taggagactt
     1741 tccaatcgaa gtggatacac ttgttgtagg atccggtccc ggaggatacg ttgctgcaat
     1801 ccgtgcagct cagttagggc agaaagtcac gatcgtcgaa aaagagaatc tcggcggcgt
     1861 atgtctgaac gtaggttgca ttccttcgaa agcccttatt tcagtagggc atcgttacgc
     1921 atccgcaaaa gattcagatg atatgggaat cacagcaact gacgtgaaac tcgacttctc
     1981 taaagcgcag gcattcaaag acggcgtagt caaaaaactg acaggcggcg ttgaaggact
     2041 tctcaaaggg aacaaagtgg atattgtcaa aggcgaagct tacttcgttg atgcgaacac
     2101 cgcaaaagtg atggatgaga catcttccca aacgtacaag tttaaaaatg caatcatcgc
     2161 aactggttca cgtccggttg aaatcccttc attcaagtat acgaaacgcg tcatcaattc
     2221 tacaggcgcg cttaacttgg aagaagttcc tggtcaccta gtcgtcatcg gcggcggtta
     2281 tatcggaact gagcttggtt cagcctatgc gaaccttggc gctaaagtga caatcatcga
     2341 aggagccgac gatattcttg cgggcttcga aaaacaaatg acgcagatcg ttaaaaaagg
     2401 attgaagaaa aaaggcgtcg aaatcgttgt gaaagcatct gcaaaaggtg ttgaagaatc
     2461 cgacactggc gttgtcgtca cgtatgaagc aaaaggcgaa gaacaaaagg tcgaggcgga
     2521 ttatgtcctt gtcacggttg gacgccgtcc gaacacggat gaaatcggtc ttgaagaaat
     2581 gggcgtcaaa ttccttgaac gcggtctgat cgatgttgat aaacaatgcc gtacgagcat
     2641 tccaaacatc tatgcaatcg gtgatatcgt ttccggtcca caacttgcgc ataaagcttc
     2701 ctatgaagcg aaagtggcag cagaagctat ctccggcgag aaatccgaag ttgactatat
     2761 ggctatcccg gcagtctgct tcacagatcc agagcttgca acagtcggct tgaatgagca
     2821 acaagctaaa gatgaaggct atgaagtagt agcgggcaaa ttcccgtttg ctgcaaatgg
     2881 ccgtgcactt gcgcttaacg caactgatgg attcgtcaaa cttgtatcac gtaaagaaga
     2941 cggacttcta ttaggtgcac aaattgtcgg tgaaaatgca tctgacatga ttgcggagct
     3001 tggtcttgca atcgaagctg gaatgacatt ggaagacatt gcaatgacaa tccacgctca
     3061 cccgacactc ggtgaaattt ccatggaagc tgcagaagtg gctttgggta aaccgattca
     3121 tatgatcact aaataattat agatagaaag atagcgtccg gaggcaggaa gtgcctttcg
     3181 gacgcttttt tcatttcggc acccggttcg tttgaacaag cattcaaact cgtgatagga
     3241 taaatttatt gatgaaaaat tctaatagga ttgtagcgta agacggcgtt tgcgagcaat
     3301 gttttttgcg accctaaggg atcaggatgt ggctgcctta atttctgcaa atagcgcaga
     3361 aattaaggca aatcgaaccc ttcgctgttc gattggaaag cgtccgtctg aagcggaaat
     3421 ccggtataaa ggagaggatt cgatgaataa aagtttgcta tacataccgc ttctctccat
     3481 gctgctatta gcatcatgca atgaagaagc aaaaccggaa gaaacggacc aaactgcaaa
     3541 acctgccaaa gtggaagatg tgacagatga ggtcgaaaca gatgaagaac agcgagaaga
     3601 tggagatgcc caacaggagg aggatgaagt aattaccgaa ccaacacctc tttacgagct
     3661 gaatccgaaa acatactcca ttcaaccgat cggggaagcg aaccctaaag tcgttctcct
     3721 gacgattgat gatgcgcctg ataaaagggc tcttgatatg gcgaaaacgc taaaaagctt
     3781 agaggccccc gcaatctttt tcgtcaatgg ccacttcatt acaacggatg aaaagaaagc
     3841 gatcttgaaa gaaatccatg acatggggtt catgattgga aatcatacga tgacacatcc
     3901 gaatttaaaa acattgtccg aggaagagca acgtgaagaa attatcgcgg taaatgatat
     3961 tattgaagaa gttaccggtg aacgtccact gtttttccgt gctccatttg gagcgaatac
     4021 agattttagc agagcgcttg caatagacga agggatgcaa ttgatgaact ggtcctatgg
     4081 ctatgatttc gaaaaagaat atatgacgaa ggaaaaaatt gcggatatta tggtgaatac
     4141 ggaacttctt cggaacggtt ccaacttgct catgcatgac agggattgga ctgccgatgc
     4201 cttgggagac atcgtaaatg gattgaggga taaaggatat gaatttgtgg atccgaatct
     4261 gattaaggga ttggatgagg aataactggg ggaataaggg ggaattgtag tggagtggaa
     4321 aacgagagtc acggaattgc tggggatcga ctatccaatc atccaagggg gacttgccta
     4381 tcttgcctat gcggatttag ctgctgcagt ttcagaagca gggggacttg gacagattac
     4441 agcaatgagc ttagagtctc ctgatgcgtt gcgctctgaa
//
LOCUS       DASULI010000403         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1627657,
            whole genome shotgun sequence.
ACCESSION   DASULI010000403 DASULI010000000
VERSION     DASULI010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627657"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFX34_08690"
     CDS             <1..297
                     /locus_tag="VFX34_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5565024.1"
                     /translation="IIYNFLQSAKLLADGMRSFNDNCAVGIEPNREEIDSKVKNSLML
                     VTALNPYIGYENAAKIAKTAHKEGTTLKEAALKTGLLTAEQFDEYVDPSKMIGR"
     gene            299..712
                     /locus_tag="VFX34_08695"
     CDS             299..712
                     /locus_tag="VFX34_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX5565025.1"
                     /translation="MYEENCPYCNLTADPEQQIIFETETCAYIQKPSEQKVLEGSGLI
                     VPKRHAVDVFSLTAVEWRDTQELLLKAKVYLEERYDHEGYSVGWNTGEVGGQSIPHAH
                     LHVIPRFADEPLAGKGIRHWIKQKENQRNAMSTKL"
     gene            775..1272
                     /locus_tag="VFX34_08700"
     CDS             775..1272
                     /locus_tag="VFX34_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HEX5565026.1"
                     /translation="MKKIMPFMLILMLILSACGEKAQQHSEDEHLEVGPEKHLPNGDL
                     QEVTESAAVLPKFLDDKPEDMRLVYQVAASASDVLAYMPCYCGCGESAGHGSNLNCFV
                     DEIREDGSVVWDDHGTRCLVCLEIAVESVQMKQEGKSMKEIREFIDEKYKEGYAEPTD
                     TPMPA"
     gene            1581..2102
                     /locus_tag="VFX34_08705"
     CDS             1581..2102
                     /locus_tag="VFX34_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5565027.1"
                     /translation="MVFEGNNKIRRKIYQTIDGMTDEEFNGTPSTGGWSPKQILEHLA
                     LMETYIASKISKELKNPASPKASKKMIVLSASLLVKGDLPLQTRPTDTYKTISEIKEE
                     LHNSRIYLLDVYDGSRKEHLREKSFKHPNLGLIPLEQWFPVIGLYEKCHLKRLKKTID
                     ELRINGCSLEAAE"
BASE COUNT          708 a          506 c          548 g          541 t
ORIGIN      
        1 atcatctaca atttcctgca atctgcaaag cttctcgcgg acggcatgcg cagcttcaat
       61 gacaattgtg cggtcggcat cgaaccgaac cgggaagaga ttgatagcaa agtgaaaaac
      121 tcacttatgc ttgtaacggc attgaatccg tatatcggtt atgaaaacgc agcgaaaatc
      181 gcgaagacag ctcataaaga aggcacgaca ttgaaggaag cggcgcttaa aaccggtcta
      241 ttgacagcag agcaattcga tgaatacgtc gatccttcga agatgatcgg gcgatgacat
      301 gtacgaggaa aattgcccgt actgtaactt gaccgcagat cctgagcagc aaatcatttt
      361 tgaaacagaa acttgcgctt atatccaaaa accttccgag caaaaagtgc tggaaggcag
      421 cggcttgatc gtaccgaaac ggcatgcagt cgatgtgttt tcattaacag ctgtagagtg
      481 gcgggatacg caggagcttc tgttaaaagc gaaggtctat ttggaagagc gatatgatca
      541 cgaaggctat tcggtcggct ggaatacagg cgaagtgggc gggcagtcga ttccgcacgc
      601 ccacttgcat gtcatccctc gctttgcgga cgaaccgtta gccgggaaag gcatccgcca
      661 ctggatcaaa cagaaggaaa atcaacgtaa cgcaatgtcc acaaagttgt aacctactcc
      721 aagccgattt ccgctatgat agaaggacaa ggaacttaaa aggagtcgac atgaatgaag
      781 aaaatcatgc ctttcatgct tatactgatg cttatcctgt ccgcttgcgg agaaaaggcg
      841 caacagcact cggaagatga acatttggaa gtgggccctg aaaaacactt gccgaatggc
      901 gatctgcagg aagtgaccga atcggctgcc gttctgccga agttcttaga tgacaagccg
      961 gaagacatgc gtctcgtgta ccaagtggcg gcatccgcct cggatgtact tgcctatatg
     1021 ccgtgttact gcggctgcgg tgaaagtgca ggacacggaa gcaacttgaa ctgtttcgtc
     1081 gatgaaatcc gcgaagacgg atctgtcgta tgggacgacc acggcacgcg ctgcctcgtc
     1141 tgcctggaaa tcgcagttga atccgtgcaa atgaaacaag aaggaaaatc gatgaaggaa
     1201 attcgtgaat tcatcgatga aaaatacaaa gaaggctacg cagaaccgac agacacgccg
     1261 atgcctgcat gatcaagccg ctccatccgc tctggatgga gcgtttttta caatccgcgc
     1321 ataaactcat ggaaagaagc ataaattgct ccagggacgc ataaatcatc gaagatacgc
     1381 atatatcccg aaaatgcttc ataaacgcca ataaataagc ataaatcctt tcattaacgc
     1441 ataaatgatc tcagttacaa atattaccaa caacttattt ttctgaatat gattctatca
     1501 ctttaatttt tcttcaaaac gtattatgct gtgggagtaa attgagtgcc cctaattcgt
     1561 tctacatgag gagagatata atggtctttg aggggaataa taagatccgc agaaaaatct
     1621 atcaaaccat cgatggcatg accgatgaag agttcaacgg aactccgtca acgggcggct
     1681 ggtctccgaa gcagattttg gagcatcttg cattgatgga aacatatata gcttcaaaaa
     1741 tttccaaaga attgaagaat ccggcgagcc cgaaagcatc caagaaaatg atcgtccttt
     1801 cagcgagtct gcttgtcaaa ggtgaccttc ccttgcaaac tcgcccgaca gatacgtaca
     1861 agacgatttc agaaatcaaa gaggagctgc ataactcaag gatttatttg ctcgacgtct
     1921 acgatggcag ccgcaaggaa cacttgcgcg agaagtcgtt caagcatccg aatctcggct
     1981 tgattccatt ggaacaatgg tttcctgtca tcggcttgta cgaaaaatgc catttgaagc
     2041 gattaaagaa aacgattgac gaattgcgaa taaacggctg ctccttggaa gcggctgaat
     2101 aaggaaagtc cccgaaacaa tagcgtaggg gacttttttg attcaattat tatcgaatgt
     2161 atagatcgtt caccaacaga acgcatcacg atcatgtctc aatcgtatgg acggttgatt
     2221 tgtcatcatt gttttccgtc attatgctat gatcttagca atataacgtg aggggacgtg
     2281 ttgcaaatga agaacgaacc att
//
LOCUS       DASULI010000404         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_382527, whole
            genome shotgun sequence.
ACCESSION   DASULI010000404 DASULI010000000
VERSION     DASULI010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382527"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1510
                     /locus_tag="VFX34_08710"
     CDS             2..1510
                     /locus_tag="VFX34_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX5565028.1"
                     /translation="MQKIILTKSFEQDWIRMFTHLANLFFEQSKVVNDTEEEGILVDI
                     SINKDGETIIGHAHLTQDGNEFSATFSEKVTTDNSETGTRQLKRIYSHVFLEVLEQLT
                     GMEQSWGILTGIRPMKLFHKYRQQGHNVEEAKRLLMEKHRISEEKSELLATIENVQLR
                     SIPDLHQIQNDVSIYIGIPFCPTKCAYCTFPAYAIHRKNGRVESFLDGLHEEIREIGK
                     WLTERDIKITTIYFGGGTPTSIEADEMDALYQTMYDSFPHMKEVREVTVEAGRPDTIT
                     PAKIEVLKKWGIDRISVNPQSYTDETLKAIGRHHSVQETIEKFWLSRNMGMKNINMDL
                     IIGLPNEGLEEFSHSLDETAKMQPESLTVHTLSFKRASEMTRNKEKYKVADRETVEQM
                     MKMGEQWTKENGYVPYYLYRQKNILGNLENVGYAKPGEESLYNIIIMEEVQTIIGIGC
                     GASSKFMDPATGKITQFQNPKDPAAYILTFEEYIEKKIEHLERIFPQAIRVD"
     gene            1638..>2295
                     /locus_tag="VFX34_08715"
     CDS             1638..>2295
                     /locus_tag="VFX34_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5565029.1"
                     /translation="MWSTIELTIKGRLAQLTMNRPDSMNAMDNIMMKELADAFESLSA
                     NDEVQVLIINGAGRTFSAGGDIKKMIDPDAPMDIGEVMIDVSRLAKALYKLPQITIAA
                     IHGSAAGLGFSLALGCDIILAEENSKLAMNFIGIGLIPDGAGHFFLKERIGVPEAKQL
                     IWSGEVMNAEVAMGKGLIDEVVPDGSVGQASAMLAQKFLSAPIAAMLASKKILHEQKL
                     G"
BASE COUNT          720 a          492 c          561 g          522 t
ORIGIN      
        1 catgcaaaaa ataatcctta caaaatcatt cgaacaagat tggatacgga tgttcacgca
       61 tctcgccaac ctattcttcg aacaatcaaa agtcgtcaac gacaccgaag aagagggcat
      121 tcttgtcgat atatccataa ataaagacgg tgaaacaata attggccacg cccatttaac
      181 acaggatggc aatgaattca gtgccacttt ttccgagaag gtgacaaccg acaattcgga
      241 aactgggaca cgccagctga aaaggattta ttcccacgtg ttccttgagg ttttggagca
      301 gctcactgga atggaacaat cttggggcat cctgacagga atccgtccga tgaaactttt
      361 ccataaatac cgccagcaag gtcacaacgt cgaagaggca aaacggcttc taatggagaa
      421 gcaccggatt tccgaagaga aaagcgaact tctcgcaacg attgaaaacg ttcaattgcg
      481 gtcgatcccg gatttgcatc aaattcaaaa cgacgtcagc atctacatcg gcatcccgtt
      541 ctgtcccaca aaatgcgcat actgcacatt cccggcttat gcaatccacc ggaaaaacgg
      601 gcgggtcgaa tcttttctcg acggcctgca tgaagaaatc cgcgaaatcg ggaaatggct
      661 gacagagcgg gacatcaaaa tcactaccat ctattttggc ggagggacgc cgacttccat
      721 cgaagcggac gaaatggatg ccctttacca gacgatgtat gattcattcc cgcatatgaa
      781 ggaagtaagg gaagtgaccg tcgaagcggg ccggccagac acaatcaccc ctgccaaaat
      841 cgaagtgctg aaaaaatggg gaatcgatcg gatcagcgtc aatccgcaat cgtatacaga
      901 cgaaacgttg aaagcgatcg gaaggcatca ttccgttcaa gaaacgatcg aaaaattctg
      961 gctgtccagg aatatgggca tgaaaaacat caatatggac cttatcatcg gcctgccgaa
     1021 tgaagggctg gaagaattca gccattcgct cgatgagacc gcgaagatgc agcccgaatc
     1081 gctgacggtc catacactat cattcaaacg ggcttccgag atgacgcgga acaaagagaa
     1141 atataaagtg gcggatcggg aaacggttga acagatgatg aagatgggcg agcaatggac
     1201 gaaggaaaac ggctatgtcc cgtattattt gtatcgacag aaaaatatcc tcggcaactt
     1261 ggagaatgtc gggtacgcga agcctggcga agaaagccta tacaatatca ttatcatgga
     1321 agaagtgcag acgatcatcg ggatcggttg cggagcgtcc agtaaattca tggatccggc
     1381 caccggtaaa attacgcagt tccaaaatcc gaaagatccc gctgcctata tcctgacatt
     1441 tgaagaatat atcgagaaga aaatcgaaca tcttgagagg atttttcctc aagccatacg
     1501 agtagattga aggagaatta aaccttaaca cccaaaaagt gttaaggttt ttatttcgat
     1561 tttagaagga tttttatgac gattaaacga ataagtaata atgaatgttt attcatattt
     1621 aagagggggt aattattttg tggtctacta tcgaattgac aattaaaggc aggcttgcac
     1681 agctgacaat gaatcgtccg gactccatga atgcaatgga caacataatg atgaaggaac
     1741 tggccgatgc cttcgaatct ctttcagcga atgatgaagt gcaagtattg atcatcaatg
     1801 gtgcgggccg cacattctct gcaggcgggg atattaagaa aatgatcgac ccggatgcgc
     1861 cgatggatat cggagaagtg atgattgatg tgtcacgcct cgcaaaggca ttatacaagc
     1921 ttccgcaaat tacgattgct gcaatccacg gatctgctgc aggactcggt tttagcttgg
     1981 cgctcggatg tgacatcatc ttagctgagg agaatagcaa actggcaatg aatttcatcg
     2041 gaatcggtct tataccggat ggagcgggtc atttcttcct gaaagaacga ataggagtgc
     2101 ctgaagcgaa gcaactcatt tggtcgggag aagtgatgaa cgctgaagtt gcaatgggga
     2161 aagggctcat tgacgaagtc gttccggatg gaagtgtcgg tcaagcttct gcaatgcttg
     2221 cacagaaatt cctatcggct ccaatcgcag caatgctagc ttctaaaaag attttgcatg
     2281 aacaaaaatt aggtg
//
LOCUS       DASULI010000405         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1139332,
            whole genome shotgun sequence.
ACCESSION   DASULI010000405 DASULI010000000
VERSION     DASULI010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139332"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFX34_08720"
     CDS             complement(<1..317)
                     /locus_tag="VFX34_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995353.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-diphosphate and nicotinate from nicotinate
                     D-ribonucleotide and diphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX5565030.1"
                     /translation="MSSKYADDSLALHTDLYQINMMESYWADGIHERKAVFELYFRSL
                     PFGNGYAIFAGLERILDYLKDFHFSESDLTYLREELGYKEDFLAYLKDVRFTGDVYSM
                     KEG"
     gene            complement(409..1746)
                     /locus_tag="VFX34_08725"
     CDS             complement(409..1746)
                     /locus_tag="VFX34_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5565031.1"
                     /translation="MAKQYDFTEGPLAKQLIFYSAPIILTNLLQVSYQFIDSLWVGNL
                     IGADALGAVAISGAVVFTVLSFVIGLNNAALTILSQQKGRGSETGLRNYLNAFVVMLA
                     IMSVVLSVAGFFLAKPILALLGTPDSMIEMAASYLRINFFGILFLFGYNFINTVFRSV
                     GDSKTPIYFVLTAVLLNTVLDPIFIAVFGWGVQGAAIATIVSQGVAFATGAIYSVRKQ
                     LVPFSKPSLPSKREVLDILKLGIPAGLQMSVISAGVMAIMSVVASFGPAVVAGYGAAQ
                     RLDSLIMLPAQAIGTAVNSMAGQNIGAGKWNRVHRITFIGFLYNLGIMFAIAGIVFAF
                     AGAGIRLFISDEDAVRFGADYLKMIAFFYPFLGINFVLNGAVRASGAMFQVLVLNIIS
                     FWILRYPVTYFLSGFMGEKGIAVGIGVSFVISSIIAVLYYRFGKWKKAEVFGA"
     gene            complement(1929..2243)
                     /locus_tag="VFX34_08730"
     CDS             complement(1929..2243)
                     /locus_tag="VFX34_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002015033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX5565032.1"
                     /translation="MAWIYLVIASFGEIFGVMAINLYLQKRSWPRLLLIVVTFSVGFW
                     FLSLAMRDIPMGTAYAIWTGLGAAGAVLMGILFFKEAAGWKRILFLCLIIGGAVGLKV
                     FG"
     gene            complement(2245..2583)
                     /locus_tag="VFX34_08735"
     CDS             complement(2245..2583)
                     /locus_tag="VFX34_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX5565033.1"
                     /translation="MAWFYVLVAAIVEIFWVIGLRYSDTALEWAGTVIAIIISFYAII
                     KACETLPSGTVYAVFTGSGAAAIVLIDFLFFHAEFSIAKVIFIAIIIIGVIGIKMTTV
                     ETDAAAEGGD"
     regulatory      2721..2828
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2908..>3996
                     /locus_tag="VFX34_08740"
     CDS             2908..>3996
                     /locus_tag="VFX34_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010649678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HEX5565034.1"
                     /translation="MTKQSINTKLVQLGNQSDPRTGAVNPPLHFSTAYKHDGLGLSTG
                     YDYTRTKNPTRTILEEGIANLESGDRGYACSSGMAAIQLVLSLFKSGDELIAPDDLYG
                     GTFRLFDQYADLYNIRTSYSSFENVEETEKLITENTKAIFIETPTNPLMLEIDIEQYV
                     ELARKHGLLVIVDNTFLTPYFQRPIELGADIVIHSATKYIGGHNDVLAGLVVAKGEEL
                     CNRLGENHNAIGATLSPFDSWLVIRGLKTLHVRMKQHDANAKELVAYLEDEPLVDDVL
                     YAGKGGMISFRVRESEWVDPLLQNLKLITFAESLGGVESLITYPTTQTHADIPEEERE
                     RRGVDRRLLRFSVGLEEAADLIADLKQAI"
BASE COUNT         1134 a         1106 c          895 g          861 t
ORIGIN      
        1 tccccttcct tcatcgaata aacatcgccc gtgaaacgga cgtccttcaa ataggcgagg
       61 aaatcctcct tgtagccgag ctcctccctc agataggtca ggtcgctttc actgaaatgg
      121 aaatccttca agtaatcgag gatccgctca agccctgcaa aaatcgcata gccgttcccg
      181 aacggcaagc ttctgaaata cagctcgaac accgctttcc gttcgtgaat cccatccgcc
      241 caatatgatt ccatcatgtt gatttgataa agatctgtat gtaatgccaa actgtcatct
      301 gcatatttcg aactcatgcc aaatctcctt cttccgcatt cttctgattg ttccagtata
      361 cactatttac ccgcattccg attgtaaata acctttgttc cgatcaattc acgccccgaa
      421 aacctcggct ttcttccact ttccgaaccg gtaatagagg actgcgatga tgctgctaat
      481 gacaaagctg acgccgatcc cgacggctat ccccttctct cccatgaagc ccgacaggaa
      541 atacgtgacg gggtagcgca atatccaaaa cgaaatgata ttgagcacga gcacttggaa
      601 cattgctccc gacgcgcgta cagccccgtt caacacaaaa ttgataccta agaaagggta
      661 gaaaaatgca atcatcttta aatagtctgc cccgaaacgg acagcatctt catcggaaat
      721 gaaaaggcgg ataccggcac ccgcaaaagc gaagacaatc cccgcaatcg cgaacatgat
      781 gccaagatta taaagaaaac cgatgaacgt gatcctgtgg acgcggttcc atttccccgc
      841 tccgatattc tgccccgcca tgctattcac tgccgtcccg attgcctgcg ccggcagcat
      901 gatcagactg tcaagccgct gcgcagcccc gtacccggca acaaccgcag ggccgaaaga
      961 ggcgacgaca ctcatgatcg ccatgacccc ggccgaaatg acactcattt gcagtcccgc
     1021 cggtatgccc aatttcaaaa tatcaagcac ttcccgcttc gacggcaatg acggcttcga
     1081 aaatggcacc aattgctttc ggacggaata aatggcacct gtcgcaaatg cgaccccttg
     1141 ggaaacgatc gttgcaatcg cagccccttg cacgccccat ccgaagacgg caatgaaaat
     1201 cggatccagc accgtattca acaggacggc ggtcaacacg aaatagattg gcgtcttcga
     1261 atccccgaca gacctgaata ccgtattgat gaagttgtat ccgaacagga acaggatccc
     1321 gaaaaagttg atacgcaaat aggatgccgc catttcgatc atcgaatctg gcgtcccgag
     1381 caacgctaag atcggcttcg ccaaaaagaa gcccgcaaca cttaacacaa cggacatgat
     1441 tgcaagcatg acgacaaatg cgttcaaata attgcgcaat cccgtctcgc ttccccttcc
     1501 cttttgctgg gacaggatgg tgagcgccgc gttgttcagc ccgatgacga aggataggac
     1561 agtgaatacg accgcgccag aaattgcgac cgccccaagc gcatccgcgc cgatgagatt
     1621 cccgacccaa aggctgtcga tgaattgata tgacacttgc aataaattcg tcaagatgat
     1681 cggagccgaa taaaagatca actgcttcgc aagcggccct tccgtaaaat catattgttt
     1741 cgccacagcg ctcccccttt catacaacct ccatatatac gaccgcctga ttatgcggaa
     1801 caaccgccta ttaacactgg gtaaccgcct attaacatcg gacgaacgcc tattaactcc
     1861 gaatgaccgc ctattaattc catactttgg acgcgattaa caaaaagaaa aattcgcctg
     1921 tcatcaaatc atccaaacac tttcaaacca accgcccctc cgatgatcaa acagaggaat
     1981 agaatccgtt tccacccggc cgcttctttg aaaaacaaga tgcccatcag aaccgcaccc
     2041 gccgcaccga gtcccgtcca aatcgcatag gccgtcccca tcggaatgtc acgcattgcg
     2101 agtgacagaa accagaaacc gaccgaaaac gttacgacaa tcagcagcaa acgaggccaa
     2161 gatcttttct gcaaataaag attgatagcc atgacgccaa aaatctctcc gaaactcgcg
     2221 atgactaaat agatccacgc caactcaatc gcccccttcc gctgccgcat cggtttcgac
     2281 cgtcgtcatt ttgatcccga tgacgccgat gatgataatc gcgatgaaga tcaccttggc
     2341 aattgaaaac tccgcatgga aaaacagaaa gtcgatgagg acgattgccg ccgcaccaga
     2401 cccggtgaac acggcgtaca ccgtcccgga cggcagtgtc tcgcaagctt ttatgattgc
     2461 gtagaaactg ataatgattg caatgacggt gcccgcccat tcgagtgcag tatcggaata
     2521 ccgaagtccg atcacccaaa agatctcaac gatcgccgcc actaaaacat aaaaccaagc
     2581 caatggaaat cctcctcatc attcaacatg cccccattat agcaaaaggt cccggacatt
     2641 catttccgaa acttcttgca atcttacagg aagcatgata actttagata aatcattttt
     2701 ctaacaatta catattttga ttcttatcac gagagacgga gggattggcc cgatgaagtc
     2761 tcagcaacca gcccgaattt ggtatggtgc taattccaaa aggcgcacaa acgccttgaa
     2821 gataagaaga ttgatttcaa caacagttgg aggccctctt cttattggag aggcctcttt
     2881 tttaatagag taaaggagat ggacgaattg acaaagcaga gcattaacac aaaattggta
     2941 caactgggaa atcagagcga tccgagaaca ggggcagtca atcctcccct tcatttctcg
     3001 actgcttata agcatgacgg attggggctt tccacgggct atgactacac acgtacaaaa
     3061 aatccgactc ggacaatcct cgaggaaggc atcgctaatc tcgagagcgg cgaccgcggc
     3121 tatgcgtgca gctcgggcat ggcggcgatc cagctcgtcc tatccctgtt caagtcgggg
     3181 gatgaactga ttgcgccgga tgacctttac ggggggacgt tccgcttgtt cgaccaatat
     3241 gcggatctgt ataacatccg gacttcttat tcctcgtttg aaaacgtcga ggaaacggaa
     3301 aagctgatca ccgaaaacac gaaagcgatc ttcatcgaaa cgccgacgaa cccgcttatg
     3361 ctggaaatcg atatcgagca gtatgtggag cttgcgagaa agcacggctt gctcgtaatc
     3421 gtcgacaata cgtttttgac cccatatttc caacgcccga tcgagctcgg ggcggatatc
     3481 gtcatccaca gcgcgacaaa atacatcggc ggccataatg acgtgcttgc cggactcgtc
     3541 gtcgcaaagg gagaagagtt gtgcaaccgt ctaggtgaaa accataacgc gatcggggcg
     3601 acactttccc cgttcgattc atggcttgtc atccgcggtt tgaaaacatt gcatgtgcgg
     3661 atgaaacaac atgatgccaa tgcgaaggag cttgtcgcct atctggaaga cgaaccgctt
     3721 gtcgatgacg tcctctacgc aggaaaaggc ggcatgatct cgttcagggt acgggaaagc
     3781 gaatgggtcg atccgttgct gcaaaacttg aagctcatca cgttcgccga aagtctagga
     3841 ggcgtcgaaa gcttgatcac gtatcctacg acacaaaccc atgcggacat tcctgaggaa
     3901 gagcgtgagc ggcgcggtgt cgatagacgt ctattgcgct tctcggtcgg attggaagag
     3961 gctgcggatt taattgccga cttaaaacaa gccatc
//
LOCUS       DASULI010000406         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1160693,
            whole genome shotgun sequence.
ACCESSION   DASULI010000406 DASULI010000000
VERSION     DASULI010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160693"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFX34_08745"
     CDS             <1..466
                     /locus_tag="VFX34_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565035.1"
                     /translation="AATDSLTMLSQSFTPKVEFSSEFTVDPEMMTMSLISEANSIQAH
                     PVGYAVNMYYFDEDTYIQTWQVPEDLMQKVSLWKSMDISKIQKQLYTLQIWNVKTQTY
                     DALESERKLILDSIDDYITSDGKVTIKMEFINKQHGTEGQAPDIKLHGEVKK"
     gene            463..1392
                     /locus_tag="VFX34_08750"
     CDS             463..1392
                     /locus_tag="VFX34_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004224654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5565036.1"
                     /translation="MIEIKGLTKKYGPFTALDDLNLTLDEGTVFGFVGANGAGKSTTF
                     LIVSTLLQPTSGDVFINGVSVREKPSEIRKLIGYMPDFFGVYDQLKADEYLDFYGASY
                     GIPEEERKKLIPQLLELVNLSHKRYSYVDLLSRGMKQRLCLARCLIHDPKVLILDEPA
                     SGLDPRARIEMRDILKTLKSMGKTILISSHILPELAEMCDEIGVIDNGRLIAHGSVAE
                     IQKKLQGEKTITVRLAAKTEEAVSFFEEQPFVTSIEKLEGEEGISFIYRGGEVEQIDM
                     LKRAILNDLPIVSFMEHVTNLEDVFMEITKGAE"
     gene            1389..2240
                     /locus_tag="VFX34_08755"
     CDS             1389..2240
                     /locus_tag="VFX34_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5565037.1"
                     /translation="MKMNFNNPVLFKELKLRFRSFKGFNGILFFLLAMCIFVFGYIFL
                     TVNMTGTSYFRPSQSFVLFAFLSYIQLGLVLFTAPGLTAGSISSEREKQTLPILLTTS
                     QSSFQIISGKMLSSVAFLLLLIVAGLPVYSLVFLFGGISPGDFVKIFLFLFVTLLAIG
                     SIGVMFSTLIRRTVVSMIATYGTMLFLSVVTGFLFIIVIQMKAFNHMGTPVTSPSYLG
                     QFLASINPAVLFATFLSPELNRSITEMTKIDFPIWAGYLIFYGIVTAVALLIAVKKLR
                     VNMKRLK"
     gene            2254..3648
                     /locus_tag="VFX34_08760"
     CDS             2254..3648
                     /locus_tag="VFX34_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565038.1"
                     /translation="MEEKTALHRYVSRALVSLRFERSVHMLQAGLFLTALLCTSFLAA
                     SRLFILPHYEWVAFWIAVTAIAATIVALFLKNVRRDEALWKLDQYVPDNLLVTALDDR
                     VKQSDFAQAIEKRAESAIGIAYPKFKKRDKKYLNGKMLTGFLILLACTVLLLVFPSEA
                     QQEANAIAKEKEIIEELKKEVAELVKKEKDPELKKELAELVDKLKETQTTEETLRELV
                     KKQKEFKLKEQRLAEKKTASENGLSESEELELEELKSIVDQLAQQAGKAQTALNKIGK
                     APSLPALASAGIPPASGNSQTGTSSNAKPGTGQSQTGNQTGGQGQSQGEGQGEGQGQG
                     QGQVEGQGQGQGQVEGQGEGQGQGQGQGHGQGQGQGNGSGAGTGQGGRNLLSIPSERL
                     GGYKDPSVIGGELGEGEFIEENEREGLVERGTLRPYQEVVGSYKDAYLKGSDKMNLPA
                     DLQRVLSDYFSSIE"
     gene            3671..>4120
                     /locus_tag="VFX34_08765"
     CDS             3671..>4120
                     /locus_tag="VFX34_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5565039.1"
                     /translation="MSFSPEQFEEMSNTLTEVREEIGKFIVGQQEAVEFSLYSILADG
                     HALLEGLPGLGKTMLIRTISEVLDLSFSRIQFTPDLMPADITGTSILERNPEGVQRFV
                     FKEGPIFSQMVLADEINRATPKTQSALLEAMGEKTVTVLGETRKMARP"
BASE COUNT         1177 a          827 c         1057 g         1059 t
ORIGIN      
        1 agcggcaacg gattccttaa cgatgctatc acagtcattc acaccgaaag tcgaattcag
       61 cagtgaattc acggtagacc cggaaatgat gacaatgagc cttatctctg aagcgaacag
      121 catacaggcc cacccggtcg gttatgcagt gaacatgtat tattttgatg aagatacgta
      181 tatccaaacg tggcaagtac ctgaggattt gatgcaaaaa gtatctctct ggaagtcgat
      241 ggacatttcg aaaatacaaa agcagttata cactttacaa atttggaatg tcaaaacgca
      301 aacgtacgat gcacttgaat ccgaaaggaa attgatattg gattcaattg atgactatat
      361 tacgtcggat ggaaaagtga caatcaaaat ggagttcatc aataagcaac acggcactga
      421 agggcaagcg cctgatatca agttgcatgg cgaggtgaag aaatgatcga gattaaaggg
      481 ttgacgaaga aatatggtcc attcactgcg ctggatgatt tgaatctaac attggatgag
      541 ggaacggttt tcggatttgt cggtgccaat ggtgccggta aatccacaac attcctcatc
      601 gtttccacgt tattgcagcc gacttcaggc gatgtgttca tcaacggggt cagcgtgagg
      661 gaaaagccat ccgaaatccg taaactgatc ggctatatgc ctgacttttt cggagtatat
      721 gatcaattga aagccgatga atacctcgac ttttacggtg cgagctacgg aattccggaa
      781 gaggaaagga agaaacttat tccgcagctg ctggaattgg tgaatctgtc acataagagg
      841 tattcgtacg tggacctgct atcccgtggg atgaagcaaa gattatgttt ggccagatgt
      901 ctcatccatg acccgaaagt gctcatcttg gacgaaccgg cgtcagggct ggacccaagg
      961 gcgcggattg aaatgcgcga tattttgaag acactgaaaa gcatggggaa gacgattttg
     1021 atttcttcac atattttgcc tgaattggct gagatgtgtg atgaaatcgg agtcattgac
     1081 aatggcagac taatagcgca tggatcagtc gcggaaatcc agaaaaagct gcaaggcgaa
     1141 aaaacgataa ctgttcgttt ggcggcgaaa accgaggaag ccgtctcgtt ttttgaagaa
     1201 cagccatttg tcacttcgat tgaaaaacta gaaggcgaag aaggaatctc tttcatatac
     1261 aggggcgggg aagtggagca gattgacatg ctgaaacgtg cgattctcaa tgatctgccg
     1321 attgtttctt tcatggagca tgtcacgaac ttggaagatg tctttatgga aataacgaaa
     1381 ggagcggagt gaagatgaat tttaataatc cggttctttt taaagagttg aagttgagat
     1441 tccgctcgtt caaaggtttt aatggaattc ttttcttttt gttggcgatg tgcattttcg
     1501 tcttcggtta tatatttttg acggtcaata tgacggggac atcctatttc cgaccaagtc
     1561 aaagctttgt tctgttcgct ttcctttcct atatccagct agggctcgta ttgtttacag
     1621 caccaggctt gacggcaggt tcgatcagct cggaacggga gaaacagacc ttgccgatac
     1681 tgctgacaac ctcgcaaagc tcatttcaga ttatttccgg gaaaatgctc tcatctgtgg
     1741 cttttctatt gctgcttatt gttgcagggc ttcctgttta tagtttagtt ttcttgttcg
     1801 gagggatttc tccgggtgat ttcgtgaaaa tattcctctt cttgtttgtc acattgcttg
     1861 caatcggcag cataggcgtc atgttctcca ctttgatccg aagaactgtc gtttctatga
     1921 ttgccacata cggtacaatg cttttcttgt ccgtagtgac cggttttctc ttcattatcg
     1981 tcattcaaat gaaagcattt aaccatatgg gaactccggt gacctctcca tcatatcttg
     2041 gacagttttt agcctccatc aatccggcgg tgttattcgc gacattccta tcaccagagt
     2101 tgaatcggtc gataaccgaa atgacgaaga tcgattttcc aatttgggcg ggttatttga
     2161 ttttttacgg aatcgtcacc gcagtagcgc tgttgatcgc cgtaaaaaaa ctgcgtgtca
     2221 atatgaaaag attaaaatga tggggtgaga tagatggaag aaaaaacagc attgcatcga
     2281 tatgtcagta gggctctcgt ctcattgcgc ttcgaacgat ctgtccatat gctgcaagct
     2341 gggcttttct tgactgcttt gttgtgcaca tcgttccttg ctgcttccag actgttcata
     2401 ttgcctcatt atgaatgggt tgctttttgg atcgcggtca ctgcaattgc ggcgacgatt
     2461 gtcgcgctgt ttcttaagaa cgttcggcgg gatgaagctc tctggaagtt ggatcaatat
     2521 gttccggaca atctgctcgt gaccgcttta gatgatcgag tcaaacaatc cgattttgcg
     2581 caagcgattg agaagcgggc agaaagcgcc ataggaattg cttatccgaa attcaagaaa
     2641 cgggataaga aatacttgaa tggaaaaatg ctaacaggct ttcttatctt acttgcttgt
     2701 accgtcctat tgctcgtttt cccttcggaa gcacagcagg aagcaaatgc tatcgcaaaa
     2761 gaaaaggaaa tcatcgagga attgaagaag gaagtcgcgg aactggtcaa gaaggaaaag
     2821 gatccggagc taaaaaaaga attggcagag ctggtggata aactcaaaga aacacaaacg
     2881 accgaggaaa cattaaggga actggtaaaa aaacaaaagg aattcaagct gaaagaacag
     2941 cgattagccg aaaagaagac agcttccgaa aatggcttaa gcgaatccga ggaattggag
     3001 ctggaagaac ttaaatcgat tgttgaccag cttgcacaac aagcaggaaa agcgcaaact
     3061 gcattgaata aaatcggcaa agccccatcg ttgccagcct tggcaagtgc cggaatcccc
     3121 cctgcatcgg gtaatagcca aacgggcaca agctccaatg ccaaacctgg aacgggccaa
     3181 tcccagactg ggaatcagac tggcggtcag gggcagagtc aaggcgaagg ccaaggcgaa
     3241 ggccagggtc aaggtcaagg tcaagtcgaa ggccagggtc aaggtcaagg tcaagtcgaa
     3301 ggtcaaggtg aaggtcaagg gcagggccaa ggtcaagggc atggccaagg ccagggtcaa
     3361 ggcaatggat cgggggcagg aaccggccaa ggtggacgga atcttctttc catcccttcc
     3421 gagcgattag gcggttacaa agatccttcc gtcatcggcg gtgaacttgg cgaaggggag
     3481 ttcatcgagg agaacgaacg tgaaggtctt gttgaaagag gtacgttacg gccatatcag
     3541 gaagtggtcg gaagctataa agatgcctat ttgaaaggtt cggataaaat gaatctccct
     3601 gcggatttgc agcgggtcct ctccgattac ttttcatcaa ttgaatgatt aatggatggg
     3661 agttgtacaa atgtcgtttt cacctgaaca atttgaagaa atgagcaata cgctcacaga
     3721 agtacgggaa gaaatcggta aattcattgt aggacaacag gaagccgtgg aattctcgtt
     3781 atattccatt ttagcggatg ggcatgcttt gcttgagggg ttgcccgggt taggaaagac
     3841 gatgttgata cgcacaatat ccgaagtatt ggacttgtct ttttcccgta tccaatttac
     3901 gccggatctc atgcctgccg atattacggg aacgagcata ttggagagga atcctgaagg
     3961 cgtccagcga tttgtattca aagaagggcc gatcttcagt caaatggtgc ttgcggatga
     4021 aatcaaccgt gcaacaccga agacccaaag tgctttgctt gaggccatgg gtgaaaaaac
     4081 cgtgacagtg cttggggaaa ccaggaagat ggccagacct
//
LOCUS       DASULI010000407         6094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_537836, whole
            genome shotgun sequence.
ACCESSION   DASULI010000407 DASULI010000000
VERSION     DASULI010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537836"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFX34_08770"
     CDS             <1..188
                     /locus_tag="VFX34_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565040.1"
                     /translation="LQQEEVESIHAVSIDDLYALFKEEVEIIACSDGTKLRLSDFVPH
                     ETEYFNAIADMLKATTS"
     misc_binding    236..448
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            517..1791
                     /gene="serS"
                     /locus_tag="VFX34_08775"
     CDS             517..1791
                     /gene="serS"
                     /locus_tag="VFX34_08775"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5565041.1"
                     /translation="MLDIKLLRANFDEVKEKLTKRGEDLTDLDKFVGLDEKRRELIAK
                     VEVLKAERNEVSQKVAQMKRNKENADDVIARMREVGDEIKALDEQLHQVEEELNYIMM
                     RIPNIPHESVPVGDSEDDNVEIRTWGEKPEFGFEPKPHWEIGTDLNLLDFERAAKVTG
                     SRFVFYRGLGARLERALINFMLDLHIEEHGYEEMLPPYLVNRTSLTGTGQLPKFEEDA
                     FLIEEEDYFLIPTSEVPVTNYYRDEILDGAQLPVAYTAYSTNFRSEAGSAGRDTRGLI
                     RQHQFNKVELVRFVKPEESYDELEKLTGHAEKVLQLLGLPYRVLKMCTADLGFTAAKK
                     YDIEVWIPTQDTYREISSCSNFEDFQARRASIRFRREQGAKPEFVHTLNGSGLAVGRT
                     VAAILENFQQEDGSVVIPEVLRPYMGGKEVIN"
     gene            complement(1992..2492)
                     /gene="tadA"
                     /locus_tag="VFX34_08780"
     CDS             complement(1992..2492)
                     /gene="tadA"
                     /locus_tag="VFX34_08780"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX5565042.1"
                     /translation="MDVLKMDRDFMRLAIEEAKKAEGIGEVPIGAVIVKDGEVIARAH
                     NLRETSQNAVTHAELSAIQDACGALGSWRLEETTLYVTLEPCPMCAGAILQSRIPRVV
                     YGARDPKGGCVDSLYRLLNDQRFNHECEVTEGVLGDECGAMLTNFFRSLREKKKEQKK
                     AMRGID"
     gene            2624..3259
                     /locus_tag="VFX34_08785"
     CDS             2624..3259
                     /locus_tag="VFX34_08785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX5565043.1"
                     /translation="MSVPFITVEGPIGVGKTTLSKAIAEAQRFHQLSEIVDENPFLNK
                     FYEDIEEWSFQTEMFFLCNRYKQLSDIQKKFLSRQQPVVADYHIFKNLIFARRSLPAD
                     EYAKYEEIYHILTKDMPEPNVIIYLHATLDTLMKRIALRGRDFEKNIDPSYLQRLSED
                     YEQFIADFEAAHPDIPVLHFNGDEIDFVNDERDLQAILSKVDETIRKGAVQ"
     gene            3256..3924
                     /locus_tag="VFX34_08790"
     CDS             3256..3924
                     /locus_tag="VFX34_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX5565044.1"
                     /translation="MNLREKYGIPKNAVITIAGTVGAGKSTMTNALANALDFRTSLEN
                     VDKNPYLDRFYDDFEKWSFHLQIYFLAERFKEQKRIFEYGGGFIQDRSIYEDTGIFAK
                     MHKDKGTMDPVDYDTYTKLFDAMVMTPYFPHPDLLVYLDGPFEKILARIQERGRLMEQ
                     QTPLSYWEELHERYVKWIDSFNACPVLRIDITDYDLKSNPHEVETVVERIGNMLNLSA
                     TLKK"
     gene            4140..4625
                     /locus_tag="VFX34_08795"
     CDS             4140..4625
                     /locus_tag="VFX34_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5565045.1"
                     /translation="MWTRMAITAIVFKVPDLKFVLARLLRNATDMGNALRPYYGDEIA
                     DAYRDLISEHLTLAAELVTAAANGETEKAEQKEAEWYQNADKIAEFLNRINPYIGKEA
                     MRKMLYTHLALTKLEAVCMIQKNFELEVQVFDRIEAEALMMADMLSEGIFKQFPYMFV
                     P"
     gene            4789..5505
                     /locus_tag="VFX34_08800"
     CDS             4789..5505
                     /locus_tag="VFX34_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HEX5565046.1"
                     /translation="MQSISGKVALITGAGRGIGRATALAFAKEGIHVGLVGRTLENLQ
                     RVAEELKQYDVKVAYAAADVADLESISSAVESIRGELGPIDILVNNAGISKFGSFMDL
                     TPEEWTNIIDVNVKGVYYTTRAVLPEMIERNTGDIINISSTAGQKGAPLTSAYTASKA
                     AVIGMSESLMLEVRKKNIRVTTLTPSTVATDMAVELNLTDGNPEKVMQAEDLADLMVA
                     QLKLHPRVVLKHAGLWSTNP"
     gene            complement(5850..>6094)
                     /locus_tag="VFX34_08805"
     CDS             complement(5850..>6094)
                     /locus_tag="VFX34_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5565047.1"
                     /translation="DAVIVEHDYSMIVLVGEGMQYTTGLAARAATAIARSGSNIEMIN
                     QGSSEVSLVFGVKVKDETKSLKELYNEFFVKSYVQS"
BASE COUNT         1755 a         1350 c         1504 g         1485 t
ORIGIN      
        1 ctttgcagca ggaggaagta gaaagtattc acgctgtttc aatcgatgat ttgtatgcac
       61 tcttcaagga agaagtggaa atcatcgctt gctcagatgg cacgaaacta agattatccg
      121 attttgttcc acatgaaaca gaatatttta acgcaattgc ggatatgtta aaggctacaa
      181 cttcatgaac cttgcaaaaa gtggttgtat gtagtaaagt gatgttaatt tcaaattaaa
      241 gacgatgatg ggaagcagta gcaagcacgt ttttcaaaga gagccggcgg atggtgcgag
      301 ccggtaaaga cacttgtgaa tccgtccctg agtagccggg ctgaacaatc agtaagccca
      361 gccggacatt gaaccgttat ttcaaagagt ggatcagcta gcttgctggt caatttgggt
      421 ggcaacgcgg gtagttctcg tcccttgtat aggggataga gggctttttt tgtgttcaaa
      481 aatcgtcgtc aaattacatt agaggaggaa tcggctatgt tagacattaa attgcttcgt
      541 gctaactttg atgaagtgaa agagaagctt acgaagcgcg gagaagactt gacggatttg
      601 gataagttcg tcggtttgga tgaaaagcgg agagaactga ttgcgaaagt ggaagtgctg
      661 aaggcggagc gtaacgaagt ttcgcaaaaa gtggctcaaa tgaaacgcaa taaggaaaat
      721 gcggatgacg taatcgcgcg tatgcgtgaa gtcggagatg aaatcaaagc tcttgacgag
      781 caattgcatc aagtcgaaga agagttgaat tacatcatga tgcgcattcc gaacatcccg
      841 catgaatcag ttccagtagg tgacagtgaa gacgataacg tcgaaatccg tacatggggc
      901 gagaagcctg aattcggttt cgagccgaag ccgcattggg aaattgggac ggatctgaac
      961 ttgcttgatt ttgaacgtgc agcaaaagtg acgggcagcc gtttcgtgtt ctatcgcggg
     1021 cttggtgcga gactggaacg tgcgttaatc aacttcatgc ttgaccttca tatagaggag
     1081 catggttatg aggaaatgct gccgccatat ttggtgaacc gcacgagctt gacgggtacg
     1141 ggccagctgc cgaaattcga agaggatgcg ttcttgatag aagaagagga ttatttcctg
     1201 attccaacat ctgaagtgcc tgtgacgaac tattaccgtg acgaaattct cgatggcgct
     1261 caattgccgg tcgcctatac ggcgtacagc acgaacttcc gttcagaggc aggatctgca
     1321 ggccgcgata cacgcgggtt gattcgccag catcagttca ataaagtcga gctcgtccgc
     1381 tttgtgaaac cggaagagtc ttacgatgag ttggagaaat tgacagggca tgcggaaaaa
     1441 gtgctgcaat tgcttggctt gccataccgt gtcctgaaga tgtgtacagc agaccttgga
     1501 ttcacggctg cgaagaaata cgatatcgaa gtgtggattc cgacgcaaga cacatatcgt
     1561 gaaatctctt cttgctcaaa cttcgaggat ttccaggcga gacgcgcttc tatccgtttc
     1621 cgccgcgagc aaggcgcgaa gcctgaattc gttcatacgt tgaacgggag cggtttagcg
     1681 gtcggccgga cagttgcggc catccttgaa aacttccagc aggaggacgg atctgtcgtc
     1741 attccggaag tattgcgtcc gtatatgggt ggaaaagaag tcatcaacta atcacaaact
     1801 gattgaaagc gcttgtcgat gaaggcaacc taaaagcgcc acatcctgtg gctatggttg
     1861 catgactcgc ttcctacgag cccgcgcgca gcgcgaggcg gaagtgaata gaactaaagt
     1921 tcatgagctt acaacgggcg caagcaacaa agaatgcgag cgcttgtcaa cagtttgagg
     1981 cgatgtggga atcaatcgat cccccgcatc gccttttttt gttccttctt cttctcacgt
     2041 agtgacctga aaaagttcgt cagcatcgct ccgcattcat ccccaagcac gccctccgtc
     2101 acttcgcatt catgattgaa gcgctggtca ttcagcaatc ggtacaatga atcgacacag
     2161 ccgcccttcg gatcgcgtgc accgtacacg acacgtggaa tccttgattg caaaatcgct
     2221 ccggcacaca tcgggcaagg ctcgagcgtc acatacaacg tcgtctcttc caatcgccag
     2281 ctgccgagcg cgccgcatgc gtcttgtatg gcggataatt ccgcatgtgt aacggcattc
     2341 tgcgaagtct ccctcagatt atgggcacgg gcaattactt ctccgtcttt cacaatgact
     2401 gccccgatcg gaacttctcc tattccctca gctttcttcg cttcttctat tgccaaccgc
     2461 ataaagtcac ggtccatttt aagtacatcc atcattcatc gctccttgta attagtgtac
     2521 caccctacaa aaggccatgc cattttgcgg caacatctat gtttcaatat ctcgatgtgg
     2581 taaaatgaag tgcagaaatt gtcgaatcaa aggagagcta tgtatgtctg ttccgttcat
     2641 tacggtagag gggcctattg gcgtaggtaa aacgacgctg tccaaagcga ttgcggaagc
     2701 gcagcggttc catcagttaa gcgaaatagt ggatgaaaat cctttcttaa ataaatttta
     2761 cgaagacatc gaggaatgga gctttcaaac cgaaatgttc ttcctctgca accgttataa
     2821 gcaattaagc gatatccaga agaaattttt aagccggcag cagccggtcg tggcagatta
     2881 tcatatattc aagaacctca tttttgcacg gcgctcgctt ccggcagatg aatatgccaa
     2941 atacgaagag atttaccata ttttaaccaa agatatgcca gagcccaatg tcatcattta
     3001 tcttcatgca acactcgata cattgatgaa gcggattgcg cttcgcgggc gggacttcga
     3061 aaaaaatatt gatccttcct acttgcaaag gctctcagag gattatgaac agttcattgc
     3121 tgactttgaa gcagcccatc cggatatccc cgtccttcac ttcaacggtg atgaaataga
     3181 ctttgtcaat gacgaaaggg atttacaagc gattctcagc aaagtcgatg aaacgatacg
     3241 aaaaggagca gtacaatgaa tttaagagag aaatatggga ttccgaaaaa tgcggtcatc
     3301 acgattgcag gtacagttgg ggccggtaaa tcgacgatga cgaatgcact tgcgaacgcg
     3361 ttggatttcc ggacttcatt ggaaaatgta gataagaatc cataccttga tcgtttttac
     3421 gatgactttg aaaaatggag cttccatttg caaatctatt ttctagcgga acgtttcaag
     3481 gaacagaagc ggattttcga atatggcggc ggctttatcc aagaccgttc catctatgaa
     3541 gatacgggaa tcttcgccaa gatgcataag gacaaaggga cgatggatcc ggtcgattac
     3601 gatacatata cgaagctttt cgacgcaatg gtcatgaccc cgtatttccc gcatccagac
     3661 ctgcttgtct atttggatgg accattcgag aaaatccttg cacggatcca ggaaaggggt
     3721 cgcttgatgg agcagcaaac cccgctttcc tattgggaag aactgcatga gcgctacgtg
     3781 aagtggatcg attcattcaa tgcatgtccg gtgcttcgca tcgatattac ggattatgac
     3841 ttgaagagta atccccatga ggttgagacc gtcgtcgagc ggatcggcaa tatgctgaac
     3901 ttgtcggcta cattgaaaaa gtaagggaag agcggccaaa tatggccgct ttttttcatt
     3961 caccgatatc accccaatcg aataatatgt actgacccaa gtatgcatgt gggagtggtg
     4021 cagtgaatca aaattatgga accaccatgc cggtggattc aatgaggcct attaattgct
     4081 atagcaaggc acaagtggaa ttgtggaata aaaaccggct tctttgggag caacatgtca
     4141 tgtggacacg gatggccatc actgcaattg tcttcaaggt cccggatctg aaatttgtcc
     4201 tcgcgcgcct tttgcgtaat gcaacggata tggggaatgc actaagaccc tattatggag
     4261 acgaaatcgc agatgcctat cgcgacctta tttcggaaca tttgacgctt gcggcagaat
     4321 tggtgacggc ggctgcgaac ggggaaacgg aaaaggcgga gcagaaagag gccgaatggt
     4381 atcagaatgc ggacaaaatc gcggaatttc taaataggat caatccctat atcggaaaag
     4441 aagcgatgcg caaaatgctc tatacccatt tggctctaac aaagctcgaa gcggtatgca
     4501 tgatccaaaa aaatttcgag ttggaagtgc aagtgttcga ccggatcgag gcggaagctt
     4561 taatgatggc ggatatgcta tcggaaggca tattcaagca atttccgtac atgttcgtgc
     4621 catgatgtcc agggaaacgt agcttactag acaatagtgg atattacgtt tacgataaac
     4681 gtaatttatc cacttttttt atatataggc ggcaactaag cgtgagtaaa gcatatgcat
     4741 tcgttatcgt ctaaaataaa gatatctcaa taaaaggagc ggaaaaacat gcaatcaatc
     4801 tcaggaaaag tggcattaat tacaggagct ggccgcggaa tcggacgtgc tacggcattg
     4861 gcttttgcaa aagaaggaat tcacgtaggc cttgttggtc gcacacttga aaatttacaa
     4921 cgagttgctg aggaacttaa acagtatgat gtgaaggtag catacgcggc ggcagatgta
     4981 gcagatttag aatcaatttc atcagcagtc gaatcaattc gtggagagct aggtccaatc
     5041 gacattttag tgaataacgc cggcatttca aagttcggca gcttcatgga tctaacgcct
     5101 gaagaatgga cgaacattat agatgtcaac gtcaaaggcg tgtattacac aacacgcgcg
     5161 gtattgcctg aaatgatcga gcggaacaca ggcgacatca ttaatatctc atcaacggca
     5221 ggtcaaaaag gcgcgccgct cacaagtgca tacacagctt caaaagctgc agtgatcgga
     5281 atgagcgagt cattaatgct tgaagtgcgc aagaaaaata tccgtgtgac aacgttgacg
     5341 ccaagtacgg tcgcgacgga tatggctgtc gaactgaacc tgacagacgg caatccggaa
     5401 aaagtgatgc aagcagaaga tttagcagac ctaatggtcg cacagctgaa acttcatcca
     5461 cgtgttgtct tgaaacatgc ggggctttgg tcaacgaatc cttaaacaag caaaagggct
     5521 gtccagaaag tcaaaccgac tttctggaca gccctttatt gttaaatata gaaggtctaa
     5581 actcacaaga cgagcataac tggcgtagaa tgcgccataa ctaagcggaa ctcgagcata
     5641 accagctgga actcgagcaa aacgctcccc aactcgagca aaagttttcg cacgccccta
     5701 gagcgcctga taatttgcgg caaccgccta atacagctac aaaaccgctt aaaaactcgt
     5761 cagaaccgcc taatgcagct tcaagaccgc ctgaaatgcc atccgaaccg cctaatgtat
     5821 ccttaagacc gcctgaaaaa aaattacggt tacgattgca cgtaagattt cacgaagaac
     5881 tcgttataca actctttcaa actcttcgtt tcgtccttta ctttcacccc gaagacaagg
     5941 ctgacttcgg atgaaccttg gttgatcatt tcgatattgg aaccgcttct cgcaatggca
     6001 gtcgcagccc gtgcggccaa gccggtcgta tactgcatgc cttccccaac gagaacgatc
     6061 atcgaatagt catgctcgac gatgaccgca tccg
//
LOCUS       DASULI010000408         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1008569,
            whole genome shotgun sequence.
ACCESSION   DASULI010000408 DASULI010000000
VERSION     DASULI010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008569"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1181
                     /locus_tag="VFX34_08810"
     CDS             30..1181
                     /locus_tag="VFX34_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019376889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEX5565048.1"
                     /translation="MKFFHTADWHLGKLVQGVHMTEGQRHVLRQFVDAVREVKPDAVI
                     IAGDLYDRAVPPPEAVALLDEVLWEIVMELKTPVIAVAGNHDSPGRLQFGSRLMQSNG
                     FHIVGQMSGQTEPVILTDRFGEVHFHLVPYCDPSIVKHLYDDEYVVDHHTAMEKVIER
                     INGSMDAAARHVFVGHAFVTPHGEKEENTSDSERPLSIGGAEYVAAHLFAQFDYTALG
                     HLHQAHYVLNEKIRYAGSPMKYSISEEKHAKGFFIVDMDGEGNVELEKRALTPLHDMR
                     TVEGTMEEILAHPPSMDYVFVKLHDETPVLFPMEKVKSVYPNAMHIERKLVRSAAAIE
                     HQSAAATEKPDDMTLFRFFYSEMNDTEPDAETERIFAEVLQEVKGGEDK"
     gene            1178..>2457
                     /locus_tag="VFX34_08815"
     CDS             1178..>2457
                     /locus_tag="VFX34_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5565049.1"
                     /translation="MKPIQLTMTAFGPYKGTETIDFRQLEEHRLFVVSGATGAGKTTI
                     FDGICFALYGQASGEDRTEIRSMRSDFADDANQTAVELIFELNGRTYRIMRQIPYTKK
                     GNKTETNARCEFFELVDGGEVPFVDRQIVSEINRKVEELVGFTQAQFSQIVMLPQGEF
                     RKFLTSDTENKETIMRKIFKTEDYREIVNRLKTRRDEAHQQLLDEKRKVDGFINQITT
                     IVPPRESLIFRVLSQEYTNVSQVMDGLEQEIDHYSKKRTEDEMKYTAAYQKHAEMLEA
                     YHAAKKCNEQFEQLEQRKIALGKLQEEIPSVQLKEKRIADAERASVIHEIESQFRMLA
                     EEAKLKESELQKASAAVHEATKWLQERELAYKEEEGKEQERGAVAERLIRLRDHLPAV
                     TNLAAKEENVQNLKMKIGKMESEFAATVEKAESE"
BASE COUNT          752 a          490 c          663 g          552 t
ORIGIN      
        1 aatatagatt aagcaggggg atgaaagtat tgaagttttt tcacactgcc gattggcatc
       61 taggcaagct ggtgcaaggc gtccatatga cggaagggca gcggcatgtc cttcgtcaat
      121 tcgttgacgc cgtgcgcgaa gtgaagcccg atgctgtcat tattgctggc gatctttatg
      181 atcgggcggt tccgccgccg gaagctgttg cattattgga tgaagtactt tgggaaattg
      241 tcatggaact gaaaaccccg gtcattgcgg tggctggaaa ccatgatagt cccggacgtc
      301 tgcaattcgg cagcagattg atgcaatcga atggcttcca tatcgttggc caaatgtctg
      361 ggcaaactga gccggtcatt ctgacagatc gtttcggaga agtccatttc catctcgttc
      421 cctattgcga tccgtcaatt gtgaagcatt tatacgatga tgaatatgtc gtcgatcatc
      481 atacggcaat ggagaaagtc atcgagcgga ttaatggatc gatggatgcg gctgctcgac
      541 atgtattcgt gggacatgcc tttgttacgc ctcatggcga gaaagaagag aatacaagcg
      601 attcggagcg tccgctttca atcgggggtg cggaatatgt ggcagcccat ctgtttgcac
      661 agttcgatta tacggcgctc ggccacttgc atcaggcgca ttacgtcctt aatgaaaaaa
      721 tccgctatgc aggttccccg atgaaatatt caatttcgga ggaaaagcat gcaaaaggat
      781 ttttcatcgt ggacatggac ggtgaaggca atgtggaatt ggaaaagaga gcactgactc
      841 ctttgcatga tatgcggaca gtcgaaggga cgatggaaga aattctggca catccgccta
      901 gcatggatta tgtatttgtg aagctgcatg acgagacgcc ggtcttattt ccaatggaaa
      961 aagtgaagtc ggtctatccg aatgcgatgc atattgaaag gaaattggtg aggtcagcgg
     1021 cagcaattga acatcaatca gcggcagcaa cggaaaaacc ggatgatatg acactgttcc
     1081 gattctttta ttccgagatg aacgatacgg aacctgatgc cgaaacggag aggattttcg
     1141 cggaagtgtt gcaggaagtc aaagggggcg aagataaatg aagccgatcc aattgacgat
     1201 gaccgctttc ggcccatata aagggacgga aacgatcgat ttccgccagc ttgaagaaca
     1261 tcggctattt gtcgtttccg gggcgactgg cgccgggaaa acgacgatat tcgacggaat
     1321 ctgctttgca ttatatggac aggcaagcgg tgaggatcga acggaaatcc gctcaatgag
     1381 aagcgatttt gcagacgatg ccaaccagac ggccgttgaa ttgatttttg aactgaacgg
     1441 gcgcacttac agaatcatga ggcagattcc ctatacgaaa aaaggaaata aaacagagac
     1501 gaacgcgcga tgcgagtttt tcgaactggt cgatggcggg gaagttccat tcgtcgatcg
     1561 ccaaatcgtt tcggaaatca atcggaaagt tgaggagctt gtcggtttta cacaagccca
     1621 attcagccag attgtcatgt tgccccaagg tgaattcagg aaattcctaa cttctgatac
     1681 ggaaaacaaa gagacaatca tgaggaaaat cttcaaaacg gaggattacc gggaaatcgt
     1741 caatcgctta aaaacgagaa gggacgaagc gcatcaacag ttgctggacg aaaaaaggaa
     1801 agtggatgga tttatcaatc aaatcacgac tattgttcca cctagggagt cacttatctt
     1861 tcgggtatta tcacaggaat atacgaatgt ttcgcaagtg atggatggtc ttgaacagga
     1921 aatcgaccat tattccaaga aacgaacgga agatgaaatg aagtatacag ctgcctatca
     1981 gaagcatgcg gaaatgttgg aggcatacca tgcagcaaaa aaatgcaatg agcaattcga
     2041 acagctggaa cagcgtaaaa ttgcccttgg gaaactccag gaagaaatcc catcagttca
     2101 attaaaagaa aagcgtattg cggatgctga gcgggcatcc gtcatccatg aaatcgaaag
     2161 tcagttccgc atgttggccg aagaagcgaa attgaaggaa agtgaattgc aaaaagccag
     2221 tgcagccgta catgaagcga cgaaatggct gcaggaaaga gagcttgcct ataaggaaga
     2281 agaggggaaa gaacaggaaa gaggggcagt tgcagaacgt ctcatccggt tgcgcgacca
     2341 cctacctgcc gtgacgaatt tggcagcaaa ggaagaaaat gtacaaaact taaaaatgaa
     2401 aatcgggaaa atggaaagtg aattcgctgc aaccgttgaa aaggccgaat cggaaaa
//
LOCUS       DASULI010000409         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_892656, whole
            genome shotgun sequence.
ACCESSION   DASULI010000409 DASULI010000000
VERSION     DASULI010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892656"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1046
                     /gene="murB"
                     /locus_tag="VFX34_08820"
     CDS             135..1046
                     /gene="murB"
                     /locus_tag="VFX34_08820"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX5565050.1"
                     /translation="MSKHQWLKDLRGIITEGILRIDEPLNKYTMTKLGGAADLLVIPG
                     TIEETEATVRYAYDNGIPLLLLGNGSNMVVRDGGVRGIVLHLSKLDAIQVDGTKVHAE
                     AGALIIDVSRKAAAESLTGLEFACGIPGSIGGAMAMNAGAYGGEIKDVIVQCTVLTKS
                     GERIVLSKDELELGYRKSIIASEGYYVLSADFELAKGDQEEIDAKVADLTFQRESKQP
                     LEYPSAGSVFKRPPGYFAGKLIQDCGLQGKGHGGAEVSTKHAGFIINKNGATASDYIE
                     TINMVKAEVKRKFDVELELEVKIVGEE"
     gene            complement(1313..1987)
                     /locus_tag="VFX34_08825"
     CDS             complement(1313..1987)
                     /locus_tag="VFX34_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX5565051.1"
                     /translation="MGKVKGRMDESILVCVYYGPNGERLIRRGHKMASIIDCPLYILT
                     IDPLPFDEFDAEKSEYIQRWKELAEELDVEDFIVMDNEKRPVAKVIKEVAHKHNITQI
                     IIGQTAQSRWEEITKGSFMNVLLREITFVDFHVVSVDRAIKDEIEGMFEKGVRAYLVE
                     EGDGFRISFTLSKEARFEGIFFKEIGTDFNNGIFKFMNNNKMCQVQITDDTVTEATQF
                     VCKTEQ"
BASE COUNT          642 a          493 c          543 g          589 t
ORIGIN      
        1 aacatccgga aacccggtta tattttcttg tcaaaacacc tgatacccag catacttata
       61 aaagtgaagc cttctttttg attcagcaaa aaggaaggtt ttcatcgcat tagaattgaa
      121 gggactggat gttgatgtcg aagcatcagt ggttaaaaga tttacgtgga attattacag
      181 aaggcatact acggatagat gaaccattaa acaaatatac gatgacaaaa cttggcggag
      241 ctgcagatct actcgtcatt ccgggaacaa tcgaggaaac ggaagcgacg gtgcggtatg
      301 catatgataa tgggatcccg cttttattat taggaaacgg gtcaaatatg gtcgtaaggg
      361 atggcggcgt acgtggcatc gtcctccatc tgtcgaagct ggatgcgatt caagtggacg
      421 gcacgaaggt acatgccgaa gcaggggcgc tcatcatcga cgtctcaagg aaggcggctg
      481 cagaatcgtt gaccggtctt gaatttgcat gcgggattcc cggctccatc ggaggtgcga
      541 tggcaatgaa cgcgggtgct tacggcggag aaatcaagga tgtcattgta caatgcaccg
      601 tcttgacgaa atcgggcgag cgcatcgttt tatcgaagga cgaactggag ctcggatatc
      661 ggaaaagcat cattgcgtct gaaggatatt acgtcttatc cgccgatttc gagttggcga
      721 agggtgacca agaagagatt gatgccaaag tggcggactt gacattccag cgggaatcca
      781 agcagccgct cgaatatcct tctgcaggaa gtgtgttcaa acggccgccg ggctatttcg
      841 cagggaaact gatccaagat tgcggtttgc aagggaaagg ccatggcggt gcggaagtgt
      901 cgacgaagca cgccggcttc atcatcaata aaaacggagc aaccgcgtcc gattatattg
      961 aaacgatcaa tatggtgaag gcggaagtga aaaggaaatt cgatgttgaa ttggaattgg
     1021 aagtaaaaat agtcggggaa gaataagcaa acaaaagcga aagccgtaac gaagaacggc
     1081 ttttgcgagt aaaagcgaag cgttacgagc acagtgcagc gggtctagac aataacaaaa
     1141 gcggatggcg cctgcccaga ggcgacgggc ataaggcgaa gatacgaagt ggcgtttttt
     1201 gccacgcagt aactttgact tatgaccccg agcctttggc gcccggagtc tagacgataa
     1261 caaaaagcgg atgaccaaca atcggccatc cgcttctatt taagttcagt tctcattgct
     1321 ccgttttgca gacgaattgt gtcgcctcgg tgactgtgtc atccgtaatc tgcacttgac
     1381 acattttgtt attgttcatg aatttgaaga tcccattatt gaaatccgtt ccgatctcct
     1441 taaagaagat accttcgaag cgagcctctt tcgagagggt gaagctgatt cgaaacccgt
     1501 ccccttcttc gaccaaataa gcccgcacgc ctttttcaaa catgccttct atttcgtcct
     1561 tgatggcacg gtcgacagaa actacatgga aatcgacaaa agtgatttcc cgcaagagca
     1621 cgttcatgaa cgaacctttc gtgatctctt cccatctgct ttgggcagtt tgaccgataa
     1681 taatctgcgt aatattatgt ttgtgagcta cctctttgat cacctttgca accggccgtt
     1741 tttcattatc catcacgatg aaatcttcaa catcgagctc ttcggcaagc tctttccatc
     1801 tttggatata ctcggatttt tcggcgtcaa attcatcgaa tggcaatggg tcaatcgtta
     1861 atatatatag tggacaatcg atgatggatg ccattttatg tccacgtcta atgagacgtt
     1921 cgccatttgg accgtaatag acacaaacaa gtatgctttc gtccatacga ccttttactt
     1981 tgcccatgac cgtagtcacc tcttaccctt attttcgttc agttgtaacg caattttcat
     2041 ctacaatcga aaaccacaac attatacata aaagatgatg ggctgacaag atgtaaatgc
     2101 cgcattgtgc ggtaaaaata ggtttagtgt atacttcaac tatgaacttt tccaattggt
     2161 atctgtagct acctattatg tccccagttt cggcatgagt caagcccatc cccgataatc
     2221 aagaaaaatt ttacataaga agttgaaatc ccttttttca ggaggtt
//
LOCUS       DASULI010000410         7375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1199737,
            whole genome shotgun sequence.
ACCESSION   DASULI010000410 DASULI010000000
VERSION     DASULI010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199737"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFX34_08830"
     CDS             <1..381
                     /locus_tag="VFX34_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5565052.1"
                     /translation="FKWVEKNYGLVIHFNPELESKRYEGEIETVVYRICQEAVFNALK
                     YAEVDEMVVRLHETDGMLHLLVMDEGKGFDLKNTDPKGTGLGIYSMKERAELVDGRLQ
                     IRSEIGKGTMIRLEVPVAKGGVDD"
     gene            378..1025
                     /locus_tag="VFX34_08835"
     CDS             378..1025
                     /locus_tag="VFX34_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5565053.1"
                     /translation="MKIIIADDHAVVRSGFMHILNFQDDMEVVATAADGLEAYELVAK
                     HRPDILLMDLSMPPGESGLIATGKIKEDFPETKVVILTMYDDEEYLFHVLKNGASGYV
                     LKNSPDEELLTAIRTVYDGGTYIHPSMATSLVRQFVKKDFDEVETDPYKILSRREIEV
                     LPLVAKGYGNKEIAEMLYISVKTVEAHKSKIMEKLNLKSRPELVEYALRKKFLNF"
     gene            1041..1568
                     /locus_tag="VFX34_08840"
     CDS             1041..1568
                     /locus_tag="VFX34_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5565054.1"
                     /translation="MTTAKQFQFDLPALRVLENEHNYLSYLMDGWHTIVLGFERDIYK
                     ADEAKEALQTLRKLIIEFIDPLKNHTEKEEEFLFPMLSKYIGDEQGPILALEEEHEEI
                     DAYIGHFLHHTRGNLEELSLQEMKDIVKDAGEAFEVITIHFVKEQSIVFPMVINTLRI
                     TEQDQLFDELYTSII"
     gene            1620..3131
                     /locus_tag="VFX34_08845"
     CDS             1620..3131
                     /locus_tag="VFX34_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5565055.1"
                     /translation="MNKKIQLPLQTANLVVGFMVWVLISSLLPFISEDIAIPPERLAI
                     VTAVPVVLGSILRIPLGYYANIFGARIIFMVSFILLLIPVFYISETTSFTGLIIGGTF
                     LGIGGAVFSVGVTSLPKYYPKEKHGLVNGIYGMGNIGTAVSTFAAPVVATQIGWSMTV
                     KLYLVLLLVFAALNFIFGDKQETKIKTPIIEQIKGVYKNEKLWFFSLFYFITFGSFVA
                     FTVFLPNFLVTYFGLEKVDAGMRTAGFIVVATLLRPVGGWLADKFQPLFLLMGAFAGL
                     TLAAIVLAFSPGIGLYTVGSILIASMAGIGNGVIFKLVPFYFNKQAGIVNGIVSMMGG
                     LGGFFPPLLLATIHSITGSYSIGFMAFSQVALVSLVLVVWLYYMDRVSLAKEVFDSTG
                     QGILVTNPKGTILSVNPAFTKLTGYTEEEVIGSNPNLLSSGRQSKQFYTSMWSIIGEE
                     GSWQGEIWNKKKDGTEYLELLTINAVKNNSGEVERYVGTFSDITPQREGSRSH"
     gene            3183..4202
                     /locus_tag="VFX34_08850"
     CDS             3183..4202
                     /locus_tag="VFX34_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019688190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX5565056.1"
                     /translation="MGTRYSRQTLFKPIGEEGQERLKNAHVIVIGCGALGSPISETLV
                     RAGVGKLTIADRDYVELSNLQRQQLFTEQDALDGVPKVVAAERRLKEIRQDIEIRAVL
                     DHVDGLLLEQLAEDADLIMDATDNFETRLLMNDVAWKKGIPWIHGACVGSSGTVFPFV
                     PGRSACFRCLLPVLPSVNETCDTAGIIAPAVQIVAARQSAEALKWLTGNQEAMMTKLL
                     HFDVWNNTQAEAGISRIRNGQCATCGESPTYPSLHQPEGTHYAVLCGRETVQIVPDSS
                     RTLSVADGVQVAERLGTEYRTTPFFVEFHAEGYRCVLFQNGRLLIHGLKDMKQGRKLY
                     HQFFG"
     gene            4218..4730
                     /locus_tag="VFX34_08855"
     CDS             4218..4730
                     /locus_tag="VFX34_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HEX5565057.1"
                     /translation="MSNLTDKEKKKSIAIAVLTVSDTRDKDTDKSGGTIIRMAEEAGH
                     HIADYRICSDDTAEISGNVEEWIGNPAVEAIVVTGGSGIGFRDVTIETVTPYFTKTID
                     GFGELFRFLSYTEDVGSKALLSRATAGSIDEKVLFALPGSSKAVKLAMDKLILPELHH
                     IVHELTKHRK"
     gene            complement(4975..5472)
                     /gene="moaC"
                     /locus_tag="VFX34_08860"
     CDS             complement(4975..5472)
                     /gene="moaC"
                     /locus_tag="VFX34_08860"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5565058.1"
                     /translation="MSELTHFNEQGRAKMVDVSEKSVTTRTAVAASSIVVNELIHSQI
                     TEGMNKKGDVFAVAQVAAVMAAKNTSSIIPMCHPLALTGVDVRFEWQIDEPKQHYEVL
                     IEAEVKTKGLTGVEMEALTAASAAALTIYDMCKAAGKEMVIGPTMLLKKTGGKNGDFE
                     RTARE"
     gene            5534..6790
                     /gene="glp"
                     /locus_tag="VFX34_08865"
     CDS             5534..6790
                     /gene="glp"
                     /locus_tag="VFX34_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX5565059.1"
                     /translation="MVEMRKPIPVAEAVRLVMGHAAPLETEMIDLEQAYGRVLAEPII
                     AKHDVPPFDRSPYDGFAIRSLDSKGAAGDNRVSLTVIGEIGAGYIGDKPIGEKEAYRI
                     MTGAKIPDNADAVVMLEQTVETDEGFTLRKPFEAGENISYKGEDAKEGELLIEAGSLI
                     HPGTVALLATFGYAEVKVAKRPVAGILSTGTELLDVADELEPGKIRNSNGPMIRAQLK
                     RMGIDYKSYGMAADDLDACTSIVEKAIAETDLLITTGGVSVGDYDYLPAIYERLGAKV
                     LFNKVAMRPGSVTTVAVLGDKLLFGLSGNPSACFTGFELFTRPAILAMMGGTAPYMPR
                     MTAKLGEDFTKPNPFTRFIRATWTFTDGGIEAVPAGFNKSNAVSSIARGNCLIVLPSG
                     TRGYAEGDEVDILLVGSEQGVSEWTL"
     gene            6778..7299
                     /gene="mobB"
                     /locus_tag="VFX34_08870"
     CDS             6778..7299
                     /gene="mobB"
                     /locus_tag="VFX34_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX5565060.1"
                     /translation="MDTVKTLHVVGYKNSGKTTLVARWVRLLKGKGLSVAVLKHHGHG
                     GKPDMSNAATDTAQFLANGADSTLVAGGGSVQFIWNEEPEFARLKKLASMGNPDVLLI
                     EGYKGETGDKVVLLRNLEDWHTLYQLEDKLLVIGCPEISVDSPHIHSREQAAQIDEWF
                     VEWIGRGDGDETV"
BASE COUNT         1851 a         1652 c         2075 g         1797 t
ORIGIN      
        1 ttcaaatggg tggaaaaaaa ttacggactc gtcatccatt ttaatccgga gctcgaatcc
       61 aaacggtatg aaggcgaaat cgagacggtc gtctaccgga tttgccagga agccgtcttc
      121 aatgcattga aatacgcgga agttgatgaa atggtcgtcc gcttgcatga aacggatggc
      181 atgcttcatt tattggtcat ggatgaaggt aaagggttcg acttgaaaaa tacagacccg
      241 aaaggcacgg gcctaggcat ctacagcatg aaagaacgag cggaactcgt tgacggacgc
      301 cttcaaatcc gatccgaaat cgggaaaggg acgatgatcc ggttggaagt cccggttgct
      361 aaaggagggg ttgacgattg aagatcatca ttgccgatga tcatgcggtc gtccgcagcg
      421 gtttcatgca catattgaac ttccaagacg atatggaagt cgtagcgacc gccgcggacg
      481 gattggaagc gtatgaacta gtggcaaagc atcgccccga cattctgctc atggacttga
      541 gcatgcctcc cggcgagagc ggtttgattg ccacggggaa aatcaaagaa gatttccctg
      601 aaacgaaagt cgtcatcctg acgatgtatg acgatgagga ataccttttc catgtattga
      661 aaaacggcgc ttcggggtat gtattaaaaa actcgcccga cgaggaattg ctaaccgcca
      721 tccggacggt ctacgacggg ggcacgtaca tccatccatc gatggcgaca tccctcgtgc
      781 gtcaattcgt gaagaaggat ttcgacgaag tggaaacgga tccttataag atcctatctc
      841 gtagggaaat cgaagtcctt ccgttagtcg caaaagggta tgggaataaa gagattgcag
      901 aaatgctcta tatttcggtc aagacggtgg aggcgcataa atcgaaaatc atggaaaaat
      961 tgaatttgaa aagcaggcct gagcttgtgg aatatgcgtt acggaaaaag ttcctgaact
     1021 tctaagtgga ggtgcgaagg ttgacgacag cgaaacaatt ccagttcgac ttgcctgcgc
     1081 tgcgggtgct tgaaaatgaa cataattatt tatcctatct gatggacggc tggcatacga
     1141 tcgtcctcgg gttcgaacgg gatatctaca aagcggatga agcgaaggaa gctttgcaga
     1201 cattgcggaa gctgatcatc gaattcatcg atccgctgaa gaaccatacg gagaaagagg
     1261 aggaattcct ttttccgatg ctatcgaaat atatcggtga tgaacaaggc cccatccttg
     1321 cgcttgagga ggagcacgaa gagatcgatg cgtatatcgg ccacttcctc catcatacgc
     1381 gcggaaacct tgaagagctg tctttgcagg aaatgaaaga catcgtcaag gatgcgggcg
     1441 aggcatttga agtcattaca atccacttcg tcaaggagca gtcgatcgtc ttcccgatgg
     1501 tcatcaacac gctgcgcatc acggagcagg atcagttatt cgacgagttg tacacatcca
     1561 tcatttgata ggcctggtga ttaagcggtt cactactttc agaaatggac tgatatgtta
     1621 tgaataaaaa aatccaattg cctttacaga cagctaactt agtagtcggt ttcatggtat
     1681 gggtattgat ttcctccctt ttgccgttca tctcggagga tatcgccatc ccgcctgaaa
     1741 gattggcgat cgtgacagcc gtccctgtcg tgttgggatc cattttacgg attcctctag
     1801 gatactatgc aaatattttc ggagctcgga tcattttcat ggtcagcttc atcctccttc
     1861 tgattccggt gttttacata agtgaaacga catcattcac cgggctgatc atcggaggaa
     1921 cgttcctcgg catcgggggg gctgtgttct ctgtcggcgt tacttcgctg ccgaaatatt
     1981 atccgaaaga gaagcacggt ctcgtcaacg ggatctacgg gatggggaat atcggtacag
     2041 ctgtgtcgac attcgccgct ccagttgttg caacgcagat cggttggtcg atgacagtta
     2101 aactctattt ggtgctattg cttgtctttg ccgctttgaa cttcattttc ggcgacaagc
     2161 aggaaacaaa aattaaaaca ccgatcatcg aacagatcaa aggcgtctat aaaaatgaaa
     2221 agctatggtt cttttcattg ttctacttca tcacattcgg ctccttcgtt gcattcacgg
     2281 ttttcttgcc gaacttcctc gtcacgtatt tcggattgga gaaagtcgat gcgggcatgc
     2341 ggacagccgg attcatcgtc gtcgcaacat tgctgcgtcc tgtaggaggt tggcttgcag
     2401 ataaattcca accgctgttc ctattgatgg gtgcttttgc aggactgaca ttggcggcaa
     2461 tcgtattggc attctcgcct ggcatcgggc tttacacggt cggcagcatt ttgatcgcct
     2521 ccatggcggg aatcggaaac ggcgtcatct tcaagctcgt tccattctat ttcaataaac
     2581 aggcgggtat cgtgaacgga atcgtttcca tgatgggcgg actcggcggg tttttccctc
     2641 cgctattgct agcgacgatc cattccatca ccggctccta ttccatcggc ttcatggcat
     2701 tttcacaagt cgcgctcgtg agccttgttc tcgtcgtctg gctttattat atggaccggg
     2761 tgtcgctcgc gaaggaagtg ttcgattcga ctgggcaagg aattctcgtt acaaatccaa
     2821 aaggtactat tttgtcagta aatcctgcat ttactaaact tacaggctat actgaagaag
     2881 aagtgattgg aagcaacccg aatctcctca gttccggaag gcaatcgaag caattttaca
     2941 catccatgtg gagcatcatt ggcgaagaag gctcctggca aggtgaaatc tggaacaaga
     3001 agaaggacgg cacggagtat ttggaactgt tgacaatcaa tgcagtcaaa aacaattcag
     3061 gtgaagtcga acggtatgtc ggtacattca gtgacattac acctcaaaga gagggcagcc
     3121 ggtcgcactg accggaagcc ctttatttat atcttgaagt tttagttgga gggggatttt
     3181 gggtgggaac gagatattcg cgccagacgc ttttcaagcc gatcggcgaa gaagggcaag
     3241 aacgattgaa aaatgcacac gtcatcgtca tcggctgcgg ggcgctcggt tcccctatct
     3301 ccgagacgct tgtccgggcg ggcgtcggca aactgacgat tgcggaccgg gattacgtcg
     3361 aactgtccaa tttgcaaagg cagcaattat tcactgaaca ggacgcgctc gacggcgtgc
     3421 cgaaagttgt cgccgccgaa cgtcggctga aagagatccg gcaagatatc gaaatacggg
     3481 cggtgctcga ccatgtagac ggccttttgc ttgaacagct cgcggaagac gccgatctga
     3541 tcatggatgc gacggacaac tttgaaacaa ggctcctcat gaatgatgtc gcttggaaga
     3601 aggggatccc ttggattcac ggcgcatgcg tcggcagttc gggcactgta tttccgttcg
     3661 tcccgggtcg gtcggcgtgt ttccgttgtt tgctgccggt cctcccatcg gtcaatgaaa
     3721 catgcgatac ggcaggcatt attgcaccag ccgtccaaat cgttgcggcg aggcagtctg
     3781 cggaagcgtt gaaatggctg acgggcaacc aagaagcgat gatgactaag ctcctgcact
     3841 ttgatgtgtg gaataacaca caagcggaag caggcatctc gcggatccgg aacgggcaat
     3901 gtgcaacatg cggcgagtcg cctacctatc cttcattgca tcaaccggaa gggacgcatt
     3961 atgcagttct atgcggaagg gagaccgtcc agattgttcc ggacagcagc aggacattgt
     4021 ctgttgcaga cggggtgcaa gtggcggagc ggttaggtac ggaatatcgg acgacgccgt
     4081 ttttcgtgga attccatgcg gaagggtatc gctgtgtttt attccaaaat ggcaggctgc
     4141 tcatccatgg gttgaaggat atgaagcaag ggcggaagct gtatcatcaa tttttcgggt
     4201 gatagggggc ggataggttg tcgaatctta cggacaagga aaagaagaaa tccatcgcca
     4261 tcgccgtctt gacggtgagc gatacgcggg ataaagatac ggataaaagc ggtgggacga
     4321 tcatccggat ggcggaagaa gccggtcacc atatcgcgga ctatcggatt tgttcggatg
     4381 acacggcgga gatttccgga aatgtggaag agtggattgg caatccggcc gtcgaggcga
     4441 tcgtcgtaac aggcggctcg ggaatcggct tccgtgatgt gacgattgaa acggtgacac
     4501 cgtatttcac gaaaacaatc gatggattcg gcgaactgtt caggttcctg agctatacgg
     4561 aagacgtcgg ctcgaaggcg ttattgagcc gagccactgc agggtcgatt gatgaaaagg
     4621 tgttattcgc attgccgggg tcgtcgaaag cagtgaagct ggcaatggat aaactgatcc
     4681 ttccggagct tcatcatatc gtgcacgaac tgacgaagca tcggaagtga tcggtgactt
     4741 gtaaatttag attgggctgg ttatcggtca ttccggggga gttatcggtc aatttcgtac
     4801 gtttatcggt cacttctcat gagttatcgt catttagtga ctattcaccc atgaaaatcg
     4861 tggcgcgttg acgagagaag tttggagttc tcgttccatc gccaggagtt ctcgttcaac
     4921 gtcgaagagt tctcgctcta gcggcaacag ttctcattca gcgccgaaga tttctcattc
     4981 tcgcgcagta cgttcgaagt caccattttt cccgccagtc tttttcagca gcatcgtcgg
     5041 accgatgacc atctctttgc cggccgcttt gcacatatcg tagatcgtga gggctgcggc
     5101 ggatgcggcg gtcaaggctt ccatttcgac accggtgagg cctttcgtct tcacttcggc
     5161 ttctatgagc acttcgtaat gttgttttgg ttcatcaatc tgccattcga agcggacatc
     5221 gacgccggta agggctaatg ggtgacacat tggaatgatg gatgatgtgt ttttcgcagc
     5281 catgactgca gcgacttgcg caactgcgaa gacatcccct tttttattca taccttctgt
     5341 aatttgcgag tggatcaatt cattcactac aatcgatgaa gcggcgaccg cggttcttgt
     5401 cgtcaccgac ttttcggaga catcgaccat cttggcgcgc ccctgctcgt tgaaatgcgt
     5461 caattcggac attggattca atcccttcaa acatagtaca tttagtatat cagaagacag
     5521 gagaggaaac gcaatggtgg aaatgagaaa accgattcct gtggccgaag ctgtcaggct
     5581 tgtaatgggg cacgcagcgc ctttggaaac ggaaatgatc gaccttgagc aggcttacgg
     5641 aagagtgctt gccgagccga ttatcgcaaa acacgatgta cctccttttg accgatcacc
     5701 ttatgacgga tttgcaatcc ggtcgctcga ttcgaaaggg gcggctggcg ataaccgggt
     5761 ctcactaacg gtcatcggag aaatcggtgc cggatacatc ggagacaagc cgatcggtga
     5821 aaaggaagca tatcggatca tgacaggcgc aaaaattccg gataacgcgg atgcggttgt
     5881 catgctcgaa cagacggtgg agacggacga gggattcaca ctgcggaagc cgtttgaagc
     5941 gggagagaac atttcgtata aaggtgagga cgcgaaggaa ggtgagctgc ttatcgaagc
     6001 gggttctctc atccatccgg gaacggtcgc attgctcgcg acgttcggct acgcggaagt
     6061 gaaagtggcg aaacgtccgg ttgccggtat tttatcgaca ggaacggaat tgcttgatgt
     6121 tgcggatgaa cttgagccgg gcaaaatccg gaattcaaac ggcccgatga tccgcgcgca
     6181 actgaagcgg atgggcattg actacaaatc gtacgggatg gcggcggacg atttggacgc
     6241 ttgcacatcg attgtcgaga aggcgattgc agaaaccgac cttcttatta cgacgggcgg
     6301 cgtttctgtc ggtgactacg attatttgcc cgcgatttac gagcggctcg gcgcaaaggt
     6361 tttattcaac aaagtggcga tgcgcccggg gagtgtgacg acagtcgcgg ttcttggaga
     6421 taagttatta ttcggtcttt ccggaaatcc ttcggcctgc tttacaggct tcgaactgtt
     6481 tacaaggccg gcgatccttg cgatgatggg ggggacagcc ccttatatgc caaggatgac
     6541 ggcgaaattg ggggaggact ttacgaagcc gaatccgttc acgcgtttca tccgcgccac
     6601 ttggacattc acagatggcg gaattgaagc ggtgcctgcg ggttttaata aatcaaatgc
     6661 agtctcgtcg attgcccgcg ggaattgcct gatcgtactg ccgagcggaa cgaggggcta
     6721 tgccgaaggg gatgaagtcg acatcctatt ggtcggcagc gagcaaggcg tcagcgagtg
     6781 gacactgtga aaacgctcca tgtcgtcggc tataaaaata gcggcaaaac aacattggtc
     6841 gcaagatggg tccgtttatt gaagggaaaa ggtttgtcgg ttgcggtgct gaaacatcat
     6901 gggcatggcg ggaagccgga tatgagtaat gccgcgacag ataccgcaca gtttctggcg
     6961 aacggagcgg attcgactct cgtagcgggc ggcggttcag ttcaattcat ttggaatgaa
     7021 gagccggagt tcgctagact caaaaaattg gcttcgatgg gcaatccgga tgttttattg
     7081 atagaagggt ataaaggtga aacaggggat aaagtcgttt tgctgagaaa tctggaagac
     7141 tggcatacat tgtatcagtt ggaagataag ctgctcgtta tcgggtgtcc ggaaatttca
     7201 gtggatagcc cccatattca ttcacgcgag caagcggcgc agattgacga atggtttgtg
     7261 gagtggatcg gaaggggaga tggcgatgaa acagtatgaa atcgtagaaa caccgataga
     7321 ggttcaaaaa tatatggatt acgtgctgca tccttctgcg ggcgctgtca cggtg
//
LOCUS       DASULI010000411         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_928623, whole
            genome shotgun sequence.
ACCESSION   DASULI010000411 DASULI010000000
VERSION     DASULI010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928623"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFX34_08875"
     CDS             complement(<1..189)
                     /locus_tag="VFX34_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565061.1"
                     /translation="MESIEIVRKFYDETVNYEWERLNSHKVEFELTKRYLNRYIKPND
                     KILDLGGGPGKYSLYLTER"
     gene            complement(231..548)
                     /locus_tag="VFX34_08880"
     CDS             complement(231..548)
                     /locus_tag="VFX34_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HEX5565062.1"
                     /translation="MQIDKIKSHQTDQLFNAILELKDLDEVYRFFDDLCTISEVQSLA
                     QRLEVAHMLKQKKTYETIKNETGASTATISRVRRCVDYGAGGYNIVLGRLYGEGNKEE
                     EKE"
     gene            complement(567..1634)
                     /locus_tag="VFX34_08885"
     CDS             complement(567..1634)
                     /locus_tag="VFX34_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017381716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX5565063.1"
                     /translation="MKGRMGKWLIVAMLLLLIMSACSKKKIVDGIPLPVPTDKVEAGT
                     HDDDEEPVELKYRAPFTGLALEKENTLRPVLVTINNHPQARPQSGIAEADVVYEFMAE
                     GTVTRFLALYQTNLPDEIGPVRSARDYFVDLAKGLDAFYIAHGFSPEAKKMLDAQVVD
                     NINGMYYDGTLFWRSKDRKAPHNSYISRENILKGADKIGAPMEISKMPPFSFHESVEG
                     AKLGNIASTVTVRFGSDSKFHNTYTYDEEKGTYDRSAGGVKTVDKVTGNPVEVSNVLV
                     FETTYNTIDSVGRQEVDLTSGGRALLFNGGIVKELEWKAIDGFLVPIENGIPAKLVPG
                     MTWISIVSTKPGIDSSVVHTP"
     gene            complement(1669..>2485)
                     /locus_tag="VFX34_08890"
     CDS             complement(1669..>2485)
                     /locus_tag="VFX34_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5565064.1"
                     /translation="ALDAGIDPIDAYAMATYNPAKHYDMTDRHGLIATGRLANLNFLS
                     SPTNPVPTDVLSKGVWILRDGQSTGAIHEIDWSIIPQFDLSFELTKEDLQFDSKLGIE
                     MINDVITKPYEITSTVPTGDESYLVLLDRNGQWKVNTFIKGFATNVQGFASSYSNTGD
                     LILIGKSKEDIIAAFNEMKRIAGGIILTEKGETVSTLPLPIGGGLSSEPVEQLIEQEL
                     ALKEKLAERGFAHGDAIYTLLFLQSTHLPYIRITQKGLLDVMKREILVPAETR"
BASE COUNT          613 a          630 c          478 g          764 t
ORIGIN      
        1 gcgttcagtt aggtataatg aatatttacc tgggccacca ccgagatcca atatcttatc
       61 attaggctta atatatcggt tcaaatagcg ttttgtaagt tcaaattcta ctttatgact
      121 gttaagtcgt tcccactcat aattaactgt ttcatcataa aactttctca caatttcaat
      181 gctctccaaa ctaattcccc ctttattaaa taaagagtag cctctaacgg ctactctttc
      241 tcttcttctt tgtttccctc gccatacaag cgaccgagca caatattata gccgcctgct
      301 ccataatcca cacagcgacg cactcttgaa atggttgcgg tgcttgcacc tgtttcgttt
      361 ttgatcgttt catatgtttt cttctgcttt agcatatgcg ctacttcgag ccgttgcgca
      421 agtgactgaa cctcgctgat cgtacagaga tcatcaaaaa agcggtacac ctcatccaaa
      481 tcctttaact caaggatggc attgaataat tggtctgtct ggtggctctt tattttatca
      541 atttgcatga acactgcccc ctcccattac ggtgtatgga cgactgatga atcaatacct
      601 ggttttgtgg atacaattga aatccacgtc atgcccggaa caagctttgc tgggatacca
      661 ttctcgatcg gaacgaggaa gccatcgatc gctttccatt caagctcctt tacaattcct
      721 ccgttgaaga gaagtgccct cccgccggaa gtcaaatcca cctcttggcg tcccacacta
      781 tcgattgtgt tgtatgttgt ttcaaacacc agtacgttcg aaacctcaac cggatttcca
      841 gtaactttat caactgtttt cacgccaccg gcagatcgat catatgtccc tttttcttca
      901 tcgtacgtat acgtattgtg gaatttcgaa tccgaaccaa accttactgt caccgtagat
      961 gcgatattcc ctagtttagc accttcgact gactcatgga acgaaaacgg aggcatcttc
     1021 gaaatttcca tcggtgctcc gatcttgtcg gcacccttta gaatgttttc tcgagagata
     1081 tatgaattgt gcggtgcctt ccggtctttc gaacgccaga ataatgtgcc gtcatagtac
     1141 attccattta tgttatcgac cacttgggca tcaagcattt tctttgcctc tggactgaag
     1201 ccatgcgcaa tataaaacgc atctagccct ttcgctaaat ccacaaagta atcccgtgca
     1261 cttcggaccg gtccaatctc atctggaagg ttcgtttggt ataaggcaag aaatcgtgtc
     1321 actgtacctt cggccataaa ctcatataca acatcagcct cagcgattcc ggattgcggc
     1381 cgtgcttgtg gatgattgtt aatcgtaacc aaaacaggtc tcaatgtatt ctctttttcc
     1441 agtgccaatc cggtaaacgg tgctctatat ttcagttcca ccggctcttc atcgtcgtcg
     1501 tgagtccctg cctctacttt atctgttggt accggcaacg gaatcccatc tactatcttt
     1561 ttctttgaac acgctgacat gatcagcagc agcaacatgg ctacgatgag ccattttccc
     1621 attctccctt tcatccgttc acccctgtct tttagttgga atgtcccttt atctagtttc
     1681 tgctgggact agtatctcgc gtttcatgac atccaataaa cctttttgcg taatccgaat
     1741 atacggtaga tgcgtcgatt gcaggaacaa tagcgtgtaa atcgcatctc cgtgcgcaaa
     1801 cccgcgctct gccagcttct cctttagcgc cagctcctgt tctataagtt gttcgacagg
     1861 ctcggatgat aatcctccac caatcggcaa cggaagcgtg gaaactgttt cacctttttc
     1921 ggtcaggata atcccgccag caatccgctt catttcattg aaggctgcaa tgatatcttc
     1981 cttactcttt ccaataagga tcaagtcacc cgtattggaa taggaggatg caaaaccctg
     2041 aacattggtt gcgaagcctt tgatgaatgt attgactttc cattggccat ttctgtcgag
     2101 gagcactaaa tagctttcat cccctgttgg aacagtagac gtaatttcgt aaggttttgt
     2161 tataacgtca ttgatcattt caattcctaa ctttgaatca aactgaaggt cttctttcgt
     2221 caattcaaat gaaaggtcga attgcgggat gattgaccaa tcaatttcat ggattgcgcc
     2281 tgtcgattgt ccatcccgca aaatccagac gccttttgac aatacatctg tcggtaccgg
     2341 attggtcgga ctcgaaagga aattcaagtt cgccaaacgc cctgttgcaa ttaacccatg
     2401 ccgatctgtc atatcgtaat gtttcgcagg gttgtatgtt gccatagcat atgcatcaat
     2461 cgggtcaatg ccggcgtcga gtgct
//
LOCUS       DASULI010000412         8944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_989672, whole
            genome shotgun sequence.
ACCESSION   DASULI010000412 DASULI010000000
VERSION     DASULI010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989672"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..489
                     /gene="ispF"
                     /locus_tag="VFX34_08895"
     CDS             13..489
                     /gene="ispF"
                     /locus_tag="VFX34_08895"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX5565065.1"
                     /translation="MIRIGQGFDVHQFEEGRPLIIGGITIPYERGLTGHSDADVLLHT
                     VTDAALGAIGEGDIGRHFPDTDEAFKDADSAILLQKVWEIVESKGYRLGNVDCTIMAQ
                     KPKMAPHIETIRARIAELLNANVTQVNVKATTTEKLGFVGREEGIASMATILLLKD"
     gene            559..2022
                     /gene="gltX"
                     /locus_tag="VFX34_08900"
     CDS             559..2022
                     /gene="gltX"
                     /locus_tag="VFX34_08900"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006828897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5565066.1"
                     /translation="MTTEVRVRYAPSPTGHLHIGGARTALFNYLFAKHHGGKFIIRIE
                     DTDTERNIEAGELSQLDNLKWLGIHHDESVDIGGEYGPYRQMERLDLYTKYAQDMLDG
                     GHAYKCFCTPEELEAEREKQKASGIAAPMYAGTCRHLTAEEVAEKEAAGMPYTIRMRV
                     PENVTYTVDDLVRGEVSFESKDVGDWVLVKANGIPTYNFAVVIDDHLMKISHVFRGEE
                     HLTNTPKQLMVYDVFRWEYPRFGHMTLIVNEERKKLSKRDESIIQFISQYKDLGYLPE
                     AMFNFFALLGWSPGGEEEIFTRDELIKLFDESRLSKSPSMFDKTKLTWMNNQYVKKLG
                     LDEVIELTLPHLQAAGRVSKEMTEEERNWVHDLIALYQAQLSYGAEIVELTAQFFNDH
                     IEYDEQSKEVLAGEQVPEVMASFQRQLTALESFDPASIKAAIKEVQKETGHKGKNLFM
                     PIRVVTTGQTHGPELPDSIALIGKEKAIERVSKYAQA"
     misc_binding    2085..2305
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2367..3035
                     /gene="cysE"
                     /locus_tag="VFX34_08905"
     CDS             2367..3035
                     /gene="cysE"
                     /locus_tag="VFX34_08905"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX5565067.1"
                     /translation="MFRRMKEDIHCIFEQDPAARSTFEVVLTYSGLHAVWAHRIAHAF
                     YKKNLLFLARLISQISRFFTGIEIHPGATIGRRLFIDHGMGIVIGETCEIGDDVTLYQ
                     GVTLGGTGKEKGKRHPTLGNNVLVASGAKVLGSITIGENSKIGAGSVVLIDVPADSTV
                     VGIPGKVVVTNGVRVRDKHDHRNLPDPVEDTCNRLEEEIAFLKSRIEELEKKAGKTDE
                     YSNL"
     gene            3016..4413
                     /gene="cysS"
                     /locus_tag="VFX34_08910"
     CDS             3016..4413
                     /gene="cysS"
                     /locus_tag="VFX34_08910"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5565068.1"
                     /translation="MSIQIYNTLTRKKEPFIPLEEGKVKMYVCGPTVYNYIHIGNARP
                     VIVFDTVRRYLQYRGYDVNYVSNFTDVDDKIINTANELGEEVGELTERFINAYFEDVG
                     ALGCGKADAHPRVTEHIDDIIAFIQVLVDKGFAYEAQGDVYYRTRKFDGYGKLSHQSV
                     DELKVGARIEESGIKENSLDFALWKSAKEGEIAWDSPWGPGRPGWHIECSVMAREHLG
                     DTIDIHAGGQDLTFPHHENEIAQSEAMTGKQFARYWMHNGYINIDNEKMSKSLGNFIL
                     VHDIRKQIDPRVLRFFMLSVHYRHPVNFSQDLVESAANGLDRIQTAYNNLKHRLSASA
                     DLGDQQDIWTHKVDEAVRQFEDAMDDDFNTANAIAAIFELVKTANVYLLEKNTQTAVL
                     NYFISAFDRLMEVLGLPFGGEELLDDEVEGLIEERLTARRERNFSRADEIRDLLKEKG
                     IILEDTAQGTRWKRG"
     gene            4419..4841
                     /locus_tag="VFX34_08915"
     CDS             4419..4841
                     /locus_tag="VFX34_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEX5565069.1"
                     /translation="MYTLRDVDVKQLNALALAYMGDAVYEQAVREHLLRSGRVKPNIL
                     HKEATRYVSAKAQASAVKMMQETGFLTEEEEAVLRRGRNAKSGSVPKNTDVVTYNHSS
                     GFEAVLGYLYLLGRTDRVGEIIGKTMQFIENPNEEGAL"
     gene            4838..5596
                     /gene="rlmB"
                     /locus_tag="VFX34_08920"
     CDS             4838..5596
                     /gene="rlmB"
                     /locus_tag="VFX34_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5565070.1"
                     /translation="MTNQETELIGGKNPVVEALRSGRELNKIWIAEGLNKKSIGEILS
                     LAKKTGIIVQAVPKQKLDGMLDVNHQGIIASVAAYSYAELDDLFAIAKNRGEDPFFLI
                     LDELEDPHNLGSILRTADAAGVHGIVIPKRRSVGLTGVVAKASTGAIEHIPVVRVNNL
                     SQTVEELKKAGVWIAGTDAQGSQDYRSMDATLPLAIIIGSEGKGMSRILKEKCDFLYH
                     LPMVGHVTSLNASVAASLLMYEVLRKRKPQTPGS"
     gene            5593..6108
                     /locus_tag="VFX34_08925"
     CDS             5593..6108
                     /locus_tag="VFX34_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX5565071.1"
                     /translation="MKKDILLVDGYNIIGAWTELRKLKVESLAAARDRLIERMAEYKA
                     HTGWRVIVLFDAHLSRGIERKDWKHKVEVVFTRENETADERIEKMVSELSGRRVQIHV
                     ATSDLTEQWVIFAQGALRKSARELEIEMNEIDKLITKKVKEIQEERPFSKIQLSDDVA
                     EIFEKWRRGMK"
     gene            6178..6828
                     /gene="sigH"
                     /locus_tag="VFX34_08930"
     CDS             6178..6828
                     /gene="sigH"
                     /locus_tag="VFX34_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019382779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HEX5565072.1"
                     /translation="MAGNVYVQGGISDFTGLSDEELLVRIHDGSTDALDFLITKYQSF
                     VKMKARSYFMMGGDREDIIQEGMIGLYKAIRDFRTDRLASFRAFAELCITRQIITAIK
                     TATRQKHIPLNTSVSLDKPVYDEESDRTLLDVISGSVLDDPEDLMIHKEDFIHMEGEI
                     NKVLSGLEKQVLSLYLEGQSYQEISDELNRQVKSVDNALQRIKRKLERHMQMDAVH"
     gene            6966..7115
                     /gene="rpmG"
                     /locus_tag="VFX34_08935"
     CDS             6966..7115
                     /gene="rpmG"
                     /locus_tag="VFX34_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX5565073.1"
                     /translation="MSKKLILCCDRCGSRNYVIPSGNKQATVRFTVKKFCNQCNAHTE
                     HKQTI"
     gene            7155..7340
                     /gene="secE"
                     /locus_tag="VFX34_08940"
     CDS             7155..7340
                     /gene="secE"
                     /locus_tag="VFX34_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX5565074.1"
                     /translation="MGKVTGFLKDVVSEMRKVSWPKRKELTRYTIVVIATVVFMAVYF
                     GLVDLGISEVMEWYLAL"
     gene            7506..8039
                     /gene="nusG"
                     /locus_tag="VFX34_08945"
     CDS             7506..8039
                     /gene="nusG"
                     /locus_tag="VFX34_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX5565075.1"
                     /translation="MEKNWYVVHTYSGYENKVKANLEKRVETMGMQDKIFRVVIPEEQ
                     ETDFKDGKKRTVMKKTFPGYVLVELIMTDDSWYVVRNTPGVTGFIGSSGGGAKPTPLL
                     PEEVDFILKQMGMKERKAEADFVVGEVIEVLDGPFAHFQGKVEEIDQDKGKVRVSVDM
                     FGRETIMELDFGQVEKI"
     gene            8212..8637
                     /gene="rplK"
                     /locus_tag="VFX34_08950"
     CDS             8212..8637
                     /gene="rplK"
                     /locus_tag="VFX34_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX5565076.1"
                     /translation="MAKKVTKIVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKDFNA
                     RTADQAGLIIPVEITVFEDRSFTFITKTPPAAVLLKVAANLQKGSGEPNKKKVATVKR
                     DKVREIAEQKMEDLNAASVEAAMAMVEGTARSMGITIED"
     gene            8755..>8944
                     /locus_tag="VFX34_08955"
     CDS             8755..>8944
                     /locus_tag="VFX34_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5565077.1"
                     /translation="MAKRGKKFTEAAKLVDRTQVYDLKEAIELAKKTSTVNFDATVEV
                     AFRLGIDTRKNDQQIRGAV"
BASE COUNT         2604 a         1963 c         2385 g         1992 t
ORIGIN      
        1 ggaggatttt caatgattcg gataggacaa gggtttgatg tgcatcagtt tgaggaaggg
       61 cggccattga tcataggtgg cattacgatt ccttatgaaa gagggttgac ggggcattcc
      121 gacgcggatg tcctattgca cacggtgacg gacgctgcgc tcggcgcaat cggggaaggc
      181 gatatcggca ggcatttccc ggacaccgac gaagcattca aagatgcgga ttcagcgatc
      241 ctccttcaaa aagtgtggga aattgtagag tcgaaaggct accggttggg caatgtcgat
      301 tgcacgatca tggcacaaaa accgaaaatg gcgccgcata tcgaaacgat ccgcgcgcgc
      361 attgcggaat tgctgaacgc aaatgttacg caagtgaacg tcaaagcgac gacgaccgaa
      421 aaacttggat ttgtcggcag ggaagaaggg atagcctcga tggcgacgat ccttctcttg
      481 aaagactgag ccctttcaaa ttaaacgaag cagtgataaa atgacacttg aattaatcaa
      541 gaacggagat gcactcaaat gacaacagaa gtacgtgtac gttatgcgcc gagcccgaca
      601 ggccatttac atatcggcgg agcccggaca gcgttattca attacctatt cgcaaaacac
      661 catggtggaa aattcattat ccgtatcgag gatactgaca cggaacggaa tatcgaagcg
      721 ggtgaattat cacagcttga taatttgaag tggcttggaa tccatcacga cgaatccgtc
      781 gatatcggcg gggaatatgg cccttaccgc cagatggaac gccttgacct ctatacgaaa
      841 tatgctcagg acatgcttga tggagggcat gcttacaaat gtttctgtac gccggaagag
      901 ctcgaagcag aacgtgaaaa acagaaggct tcaggcattg cagcaccgat gtacgcaggc
      961 acgtgccgcc atctgactgc agaggaagtc gctgaaaagg aagctgcggg catgccgtac
     1021 acaatccgta tgcgagtgcc tgaaaacgtc acctatacag tggatgatct tgtgcgcggg
     1081 gaagtgtcat tcgaatcgaa ggacgtcggt gactgggtgc tagtgaaagc gaatggcatc
     1141 ccgacgtaca atttcgcggt cgtcatcgat gaccacttaa tgaaaatttc ccacgtcttc
     1201 cgcggtgaag agcatttgac gaatacacca aaacaattga tggtatacga cgtgttccgc
     1261 tgggagtatc cacgtttcgg gcatatgacg ttaattgtca atgaagagcg gaaaaagctt
     1321 tcaaaaaggg atgaatcaat catccagttc atttcccagt acaaggacct tggctatttg
     1381 ccggaagcga tgttcaactt ctttgcattg cttggctggt cgccgggcgg ggaagaagaa
     1441 atcttcactc gtgatgaatt gatcaagctt ttcgatgaaa gccgcctatc caaatcgcca
     1501 tccatgttcg ataagacgaa gctcacatgg atgaataacc aatacgtgaa gaagctcggc
     1561 ctggacgaag tgatcgaatt gacgttgccg catctccagg cagcgggacg cgtgagcaaa
     1621 gagatgacgg aagaagaacg caattgggtg cacgacctga ttgcccttta tcaggcgcaa
     1681 ttgagctatg gtgccgaaat cgtcgaattg accgcccaat tcttcaatga ccatattgaa
     1741 tatgatgaac aatccaagga agtccttgca ggcgaacaag ttccggaagt gatggcgtca
     1801 ttccaacgtc aattgactgc acttgaatca tttgaccctg cttcaatcaa agcggcaatc
     1861 aaagaagtgc aaaaggaaac gggccataaa gggaagaatc tattcatgcc gatccgcgtt
     1921 gtcacaactg gacagacgca cggtcctgaa ttgcctgatt ccattgcgtt gatcggcaaa
     1981 gaaaaggcga ttgaacgtgt ttcaaaatac gcccaggcgt aaaatcattt agttgacttt
     2041 aagcattatc gatgtacaat tgacgtacat tcaataaaaa agactcaaaa gctttgataa
     2101 ggagaagtac acatcgcatg ctctccagag agggtcatca ccggctgtaa gtgacccgtg
     2161 cctctgcaat gtggaaatgc ccctttgagc gccgtgccga acgaagaagt aggccggacg
     2221 cgtagccggc gttacaggcg ttaagcggga aggggttcca ctccttccaa gcggagtgga
     2281 accgcgcatt caatgcgtct ctgtctagtc atggacagag gcgctttttg tattgtgcgg
     2341 aaattagaaa cgggaggtcg aaaacagtgt ttcggaggat gaaggaagac atccattgca
     2401 tatttgaaca agatcctgct gcacgcagca catttgaggt ggtgctgacg tattccggcc
     2461 tccatgcagt atgggcgcac cggattgcgc atgcattcta taaaaagaat ttgttgtttc
     2521 ttgcaaggct catttcacag atcagccgct ttttcacagg catcgagatc catccaggcg
     2581 cgacgatcgg caggcgtcta ttcattgacc atggcatggg aattgttatt ggcgaaacgt
     2641 gtgaaatcgg tgatgacgtt accctgtacc aaggcgtgac attgggcgga acgggcaaag
     2701 aaaaaggaaa gcgccacccg actcttggca ataatgtact tgtcgcttca ggagcaaaag
     2761 tgctcggttc cattacaatc ggtgaaaaca gtaaaatcgg ggcaggctcg gtcgttttga
     2821 tcgatgtacc tgctgattcg acggtcgtcg gcataccggg caaagtcgtc gtgacgaatg
     2881 gtgtgcgggt gagggataaa catgaccacc ggaatttgcc ggaccctgtc gaggatacgt
     2941 gtaaccggtt ggaagaagaa attgcatttt tgaagagccg gattgaagaa cttgaaaaga
     3001 aagcagggaa aaccgatgag tattcaaatt tataatacat tgacacggaa gaaggaacct
     3061 ttcatcccac tggaggaagg gaaagtgaaa atgtatgtat gcggaccgac cgtctacaac
     3121 tacatccata tcggcaatgc gcgaccggtc atcgtatttg acacagtccg ccgctacttg
     3181 cagtatcggg gctatgacgt caattacgtg tccaacttta cagatgtcga tgataaaatc
     3241 atcaacacgg cgaatgagct cggcgaagag gtcggggaat tgaccgaacg attcatcaat
     3301 gcctatttcg aagatgtcgg ggctctcggc tgcggcaaag cggatgccca tccacgtgtg
     3361 acggagcata tcgatgacat catcgcgttc atccaagtgc tagtggataa agggttcgct
     3421 tacgaagcac aaggggatgt ctattaccgc acacgcaagt tcgacggcta cggcaagctg
     3481 tcccatcaat ccgtcgatga gctgaaagtc ggtgcgcgca tcgaagaaag cggcattaaa
     3541 gagaactcgc tcgactttgc gctatggaaa tcggcgaagg aaggggagat cgcctgggat
     3601 agcccatggg gccccggcag gccgggttgg catatcgagt gctccgtcat ggcgagagag
     3661 cacttgggcg atacgatcga catccatgct ggcgggcagg acttgacttt cccgcatcat
     3721 gaaaacgaaa ttgcgcagtc cgaagcgatg acaggaaagc agttcgcccg ctattggatg
     3781 cataacggct atattaatat cgataatgaa aagatgtcga agtcgctcgg aaacttcatt
     3841 cttgtccatg atatccgtaa gcagatcgat ccacgggtac ttcgattctt catgctatcc
     3901 gtccattacc gccatccggt gaacttctcg caagatctag ttgagagcgc tgcgaatgga
     3961 ctggatcgta tccaaacagc ttacaacaac ttgaagcacc ggttaagcgc gtccgctgac
     4021 cttggagatc agcaggacat ttggacgcat aaggtcgatg aagcagtccg tcaatttgaa
     4081 gacgcaatgg atgacgactt caatacggca aatgccattg cagctatttt cgagctcgtg
     4141 aaaacagcca acgtttatct gctggagaaa aacacgcaaa cagctgtcct caactatttc
     4201 atttccgcat tcgaccggct catggaagtg cttggcttgc catttggcgg agaagagcta
     4261 ttggacgatg aagtcgaagg gctgatcgag gagcgcttga ctgcccgccg cgaacggaat
     4321 ttcagccgtg cggatgaaat cagggatctt ctaaaggaaa aaggaatcat ccttgaggat
     4381 actgcgcaag gcacacggtg gaaaagagga tgaacggcat gtacacattg cgcgacgtgg
     4441 acgtgaagca gttgaacgcc cttgcgctcg cttatatggg ggatgcggtt tacgagcaag
     4501 cggtccgtga acatttactg cggtcgggcc gcgtgaagcc gaacatcctg cataaggagg
     4561 caacgcggta tgtatctgcc aaagcgcagg cgtctgccgt caagatgatg caggaaaccg
     4621 gcttccttac tgaagaggag gaagcggtcc tgcgccgcgg gcggaatgcg aagtccgggt
     4681 cggtcccgaa gaacaccgac gtcgtgacct acaaccatag ttccggattc gaggcggtac
     4741 tcggctattt atatttgctt ggcagaactg atcgggtagg ggaaatcatc ggcaagacaa
     4801 tgcagttcat cgaaaatccg aatgaggagg gcgcattatg acaaatcagg aaacggaatt
     4861 gatcggcggc aaaaaccctg ttgtggaagc actccgttca ggcagggaac tgaataagat
     4921 ctggattgcc gaagggctga ataaaaaaag catcggcgaa atcctgtcac tggctaaaaa
     4981 aacggggatc atcgtccagg cggtgccgaa acagaaactg gatggcatgc ttgacgtgaa
     5041 tcaccaaggc atcatcgcct ctgttgccgc ctattcctac gcggaactgg atgatttatt
     5101 cgcaatcgcg aaaaaccgcg gcgaggatcc attcttcctc atcctcgatg aactggaaga
     5161 tccgcataac cttggctcga tccttaggac tgcggacgct gcgggcgtgc acggcatcgt
     5221 catcccgaag cggcgttcag tcggtttgac gggagtcgtc gcgaaagcat cgacgggcgc
     5281 aatcgagcat atccctgtcg tccgcgtgaa caatctgtcg cagacggtgg aggaattgaa
     5341 gaaggcgggc gtctggattg ccggaacgga tgcacaaggc tcacaagatt accgttcgat
     5401 ggacgcgaca ttgcctctag ccatcatcat cggcagcgaa ggaaaaggga tgtcccgaat
     5461 tctaaaggag aaatgcgact tcctctatca cttgccgatg gtcggacatg taacgtcctt
     5521 gaatgcttct gttgcagcat ccttgctcat gtatgaagtg ttgcgcaaac gcaagcctca
     5581 aacgccgggg tcatgaaaaa agatatactg ctcgtcgatg gatacaacat catcggagca
     5641 tggacggaat tgaggaagtt gaaggtggaa agcctcgccg cggcccgcga ccggctcatc
     5701 gaaaggatgg ccgaatataa ggcgcataca ggctggcgtg tcattgtcct gttcgatgcg
     5761 catttgagca ggggaatcga aaggaaggat tggaagcata aagtcgaagt cgttttcacc
     5821 cgtgaaaatg aaaccgcaga tgaacgcatc gaaaagatgg tatccgagct gagcggacgc
     5881 cgagtgcaaa ttcacgtggc cacatccgat ttgacggagc aatgggtcat ttttgcacaa
     5941 ggcgccctcc ggaaatcggc tcgggaacta gaaattgaaa tgaatgaaat cgataaattg
     6001 atcacgaaga aagtgaagga aatacaagaa gaacgaccat tttcaaagat tcagctatcc
     6061 gatgatgtgg cagaaatatt tgaaaaatgg cgtcgcggaa tgaaatgaac ggttgacgca
     6121 aaatttttcc atattgtata ctgattgaaa tggcaataac gaatcggggt gtggtgattg
     6181 gcgggaaatg tatacgttca agggggtata tccgatttta ccggtttatc cgatgaagag
     6241 cttttagttc gtattcatga tggcagcacg gacgcgctgg attttcttat taccaaatat
     6301 caatctttcg tcaaaatgaa agcgcgctca tatttcatga tgggcggcga tcgggaagat
     6361 atcattcagg aagggatgat cggcctctat aaagcaatcc gcgatttcag gacggatcgg
     6421 cttgcttcat tccgtgcatt tgcggagcta tgcattacaa ggcagatcat cacagccatc
     6481 aagacggcga caagacagaa gcacattccg ctgaatacgt cagtatcatt ggacaagccc
     6541 gtgtacgacg aggaatccga ccgcactttg ctggacgtca tctccggctc tgtactagat
     6601 gatccggaag atttgatgat tcataaagaa gacttcatcc atatggaagg ggaaatcaac
     6661 aaagtactga gtgggcttga aaagcaggtg ctatcactct atttggaggg gcagtcctat
     6721 caggaaatct cggacgaatt gaatcggcaa gtcaaatccg tggataatgc attgcagcgc
     6781 atcaagcgga aactggaacg gcatatgcaa atggatgccg tgcactgaag cgtgtccgga
     6841 ccagttgcca accaatttcc atcctgttga catcgcgaat attaggtggt actctttagc
     6901 aaggcaatga ctatatactt agactgtcga caaatagtcg tacggtggaa gttcaaggtg
     6961 aagaaatgtc taaaaagctg attctatgct gtgatcgttg cgggtcaaga aactatgtca
     7021 tcccctcggg gaataaacag gcaacggtca ggttcacggt gaaaaagttt tgcaatcaat
     7081 gcaatgcaca tacagaacat aaacaaacaa tctagttgca gcaatatact tagtgttcgg
     7141 aggtctacga ttgaatgggt aaggtaaccg gctttttaaa agatgtcgtc tctgaaatgc
     7201 ggaaggtcag ctggccgaag cggaaagaat tgacacgcta tacgattgtc gtcatcgcta
     7261 cagtcgtctt catggcggtc tatttcggtc tcgtcgattt gggcatttca gaagtaatgg
     7321 aatggtatct tgcgttataa taggacagca tgaataatcc ttgttgaaaa atatcccgtc
     7381 gtctaacatg aacgggtttt ttcatttccc ggaaatatat agagtgtaat cggattatat
     7441 acggggaatt tattatgaag actttaaaag gagggacgga cacacagtcc tacgtcatta
     7501 tggaaatgga gaaaaattgg tacgtcgtcc acacatattc aggttacgaa aataaagtga
     7561 aagccaacct tgaaaaacgt gtcgagacga tgggaatgca agataaaata ttccgcgttg
     7621 tcatcccgga agaacaagaa acagacttca aggacggcaa aaaacgcaca gtcatgaaaa
     7681 aaacattccc gggatatgtc ctcgtagaac tcatcatgac agacgactct tggtacgtcg
     7741 tccggaacac gcctggcgta acaggcttca tcggttcatc cggcggaggg gcaaaaccga
     7801 caccgcttct accggaagaa gtcgatttca tcctgaaaca aatgggcatg aaagaacgaa
     7861 aagccgaagc tgacttcgta gtaggcgaag tgatcgaggt actggatgga ccgttcgcgc
     7921 acttccaggg gaaagtggaa gaaatcgatc aggacaaagg aaaagtccgc gtttccgtcg
     7981 atatgttcgg cagagagaca atcatggaac ttgactttgg acaagttgaa aaaatataag
     8041 agattaccac ttgcaagacg agcataatag tggtatcatt tcaaaggtca gactgtaaac
     8101 ataaacaggc tggacggatt aaccaaattc taccggatga aaccggcaga cacatattga
     8161 gtgggagggg caacccaatt accacatcac ggacttaagg aggtgtgtct cgtggctaaa
     8221 aaagtaacca aaatcgtaaa attgcaaatc cctgcaggta aagctaaccc agctccacca
     8281 gttggaccgg cactaggtca agcaggtgta aacatcatgg gattctgtaa agatttcaac
     8341 gcacgtacag cagatcaagc aggtctaatc attcctgttg aaataactgt atttgaggac
     8401 cgttctttca cttttattac aaaaactcca ccagcagcag tattgctgaa agttgcagct
     8461 aatcttcaaa aaggttcagg cgaaccgaac aaaaagaagg tagctactgt aaaacgtgat
     8521 aaagttcgcg aaattgcaga acaaaagatg gaggacctta acgcagcatc ggttgaagcg
     8581 gcgatggcaa tggttgaagg tactgcacgc agtatgggaa tcacgatcga agactgatcg
     8641 cgataaaccc aaaagcagtt ctttgtagtg ggggttgcgc ttgttcctcg gaacacgcaa
     8701 cccttattcg tgggaggttc aatccgttaa aaccactaga ggaggaaatt tacaatggct
     8761 aaaagaggta aaaagttcac tgaggcggca aaacttgttg accgcacaca agtgtacgac
     8821 ttgaaagaag cgatcgagct tgcaaagaaa acaagcacag tcaactttga cgcaactgtt
     8881 gaagtcgcat tccgtcttgg aatcgacact cgtaaaaacg accagcaaat ccgtggagca
     8941 gtcg
//
LOCUS       DASULI010000413         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_791508, whole
            genome shotgun sequence.
ACCESSION   DASULI010000413 DASULI010000000
VERSION     DASULI010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791508"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2622
                     /gene="uvrA"
                     /locus_tag="VFX34_08960"
     CDS             <1..2622
                     /gene="uvrA"
                     /locus_tag="VFX34_08960"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5565078.1"
                     /translation="AISIDQKTTSRNPRSTVGTVTEIYDYLRLLYARVGKPICPIHGI
                     EIASQTIEQMVDRIMEYPERTRLQVLAPIIKGRKGTHTKLLEDIKKEGYVRVRVDGEL
                     RDLDDDINLDKNKKHDIEVVIDRIVIKEGIESRLSDSIESALRLAEGQVLIDIIDGEE
                     ILFSEHHACPQCGFSIGELEPRMFSFNSPFGACPECDGLGTKLEVDPELVIPDKSRSL
                     KDHAIAAWEPTSSQYYPELLKAVAKHFGIKMNEPVSELPDEHLNIILHGSGNEKIRFR
                     YKNEFGGVRDSNIYFEGVLANIERRYKETTSDYIREQMEKYMAHRDCPMCNGYRLKEE
                     SLAVKVNGLHIAEVTRFSITEADRFFKDLSLSEKDMQIAKLILREIGERLGFLINVGL
                     DYLTLSRAAGTLSGGEAQRIRLATQIGSRLSGVLYILDEPSIGLHQRDNDRLIGTLKS
                     MRDIGNTLIVVEHDEDTMMAADYHIDIGPGAGVHGGEIDSAGTPNEVMNDPNSLTGKY
                     LSGKQFIPLPLERRTSDGRVIHIEGASENNLKDVSVDFPLGQFIAVTGVSGSGKSTLV
                     NEILYKSLAQKLIRSKQKPGHHTTVTGTEELEKVIDIDQSPIGRTPRSNPATYTSVFD
                     DIRDVYASTNEAKVRGYQKGRFSFNVKGGRCEACRGDGIIKIEMHFLPDVYVPCEVCH
                     GKRYNRETLEVRYKGKNIADVLDMTVEDALEFFENIPKISRKLQTIVDVGLGYVKLGQ
                     PATTLSGGEAQRVKLASELHRRSNGKTFYILDEPTTGLHAHDISKLLVVLQRLVETGN
                     TVLVIEHNLDVIKTVDHVIDLGPEGGDKGGQIIVTGTPEEVAACESSYTGQYLKPILE
                     RDRQRMKDMVLAAESSE"
     gene            2700..3869
                     /locus_tag="VFX34_08965"
     CDS             2700..3869
                     /locus_tag="VFX34_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX5565079.1"
                     /translation="MQNERKRILTMLENGTITTEEALTLLEKLGESKRETSQTSNTSE
                     SNTGTTDQGGTQDTTSNQRQESKGFGKQSSMDEFLQDLQRDFSQVGDRFMQFMQTAVE
                     KVKTFDFDSPLGTSVSFHEESTKDATGVTEVVVDIDNGKLNIIPTEAEEVRAAFSVRA
                     FRADSEEAARKEFADKVLFVNDGNKLRIVSHLKMVQVNVDLYIPNREYEKLSVRLLNG
                     AFKTREMNVRDIQVKTANGKVDVADTTFKTADIETVNGAIQLNGLEGETLEAETLNGR
                     VYIDGKLKDVEAQSLNGHVVVTTTDVDARKVEAKTVTGAVELYVPKEVPLSGEISTNM
                     GRLDLQLTDVERTSEQEQIFQRTLRFKKDVEGGQTPLHIFGEARTGSVLVCYTAE"
     gene            complement(3925..4491)
                     /locus_tag="VFX34_08970"
     CDS             complement(3925..4491)
                     /locus_tag="VFX34_08970"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5565080.1"
                     /translation="MIKIMVDAGHGPNTPGKRSPDGTLREYQFNVAVADRIKPKLERA
                     GITVLFSHPGEMDVPLLERTNRANQLNVDAFVSIHANAHGETWNHAEGIETYAYPGAL
                     SRTTSLAELVQRSLIMATGRKDRGVKQANFAVLRDTNMPAILVECGFMTNKKEAELLK
                     QVHYQEQCAYAIAFAIIAWVYRMKDLEL"
BASE COUNT         1368 a          944 c         1174 g         1153 t
ORIGIN      
        1 gcaatctcga tcgaccaaaa gacgacaagt cgaaatccac gctctacagt ggggacggta
       61 acggagattt atgattatct ccgtctttta tatgcacgag tcggaaagcc gatttgcccg
      121 attcatggaa ttgaaatcgc atcccagacg attgagcaga tggtggaccg aatcatggaa
      181 tatcctgaaa gaactagact tcaagtgctc gctccgataa ttaaaggcag aaaaggtaca
      241 cacacgaaac tgctcgagga cattaaaaag gaaggctacg ttcgtgtccg ggtggacgga
      301 gagcttcgtg atctagatga tgatattaat ttagataaga acaagaagca tgacattgaa
      361 gtcgtcattg accggattgt tattaaggaa ggaatcgaat caaggttaag cgattccatc
      421 gaatcagcat tacgtcttgc agaaggacag gttctcattg atattatcga tggggaggaa
      481 attctcttta gtgaacatca cgcatgtcca caatgtggtt tctctattgg ggaacttgaa
      541 ccacggatgt tctctttcaa cagtccgttt ggtgcatgtc cggagtgtga tggactcggg
      601 acgaagctgg aggtagaccc ggaactcgtc attccggata aatcacgttc tttgaaggac
      661 catgcaattg cagcatggga accgacgagt tcgcaatact atccagagtt gttgaaagcc
      721 gttgcgaagc acttcggaat taagatgaat gaaccggtca gcgagctgcc ggacgaacat
      781 ttgaatatta ttcttcatgg gtccggcaat gaaaagattc ggttccgtta taaaaatgag
      841 tttggcggtg ttcgcgatag caacatctac tttgaaggtg tcctagcgaa tattgaacgt
      901 cgctataagg aaacgacttc tgattatatc cgtgagcaga tggaaaagta tatggcgcat
      961 cgagactgtc cgatgtgtaa cggctatcga ctaaaagaag agtcgttagc cgttaaagta
     1021 aacgggctcc atatcgctga ggtaacccgt ttctcgataa cggaggcaga tcgtttcttt
     1081 aaagacctat cactatctga aaaagatatg cagattgcga agttgattct acgtgaaatc
     1141 ggggagcgtc tcgggttctt gattaatgta gggttagatt atctcactct atctcgtgcc
     1201 gcaggaacct tatcgggcgg ggaagcacaa cgaatccgtt tggcaaccca aattggctct
     1261 cgactatcgg gtgttcttta tattctggac gaaccttcca tcggattgca tcagcgagat
     1321 aatgaccggt tgattggcac tcttaagagt atgagagata tcggaaatac gttaattgtt
     1381 gtggagcacg atgaagatac gatgatggca gcggattacc acattgacat aggaccagga
     1441 gctggtgtcc atggagggga aattgattct gcgggtacac cgaatgaggt gatgaatgat
     1501 ccgaattcat taacaggaaa gtatctaagt gggaagcagt tcattccatt gccgcttgag
     1561 agaagaacgt ctgatggccg agtcatccac atagaagggg cttccgaaaa caacttgaaa
     1621 gatgtgtcgg ttgatttccc acttggtcag tttatcgctg tgacgggtgt ttcgggatca
     1681 ggaaaaagta cccttgtgaa tgaaatcctc tataaatcac ttgctcaaaa actcattcgg
     1741 tcgaaacaaa agccaggtca tcatacgact gtgacaggga cggaagagct cgagaaagta
     1801 attgatattg accagtcccc aattggacgg acgccaagat ctaatccagc aacatacaca
     1861 agcgtgttcg atgacattcg tgatgtctat gcttccacga atgaagcgaa agtccgggga
     1921 tatcagaagg gccggttcag ctttaatgtg aagggcggtc gctgtgaagc atgccgcggg
     1981 gatggaatta tcaagattga aatgcacttc ttgcctgacg tatacgttcc atgtgaagtg
     2041 tgccatggaa aacgatacaa ccgggaaacg cttgaagtcc gctataaagg gaagaacatt
     2101 gcagatgtgc tagatatgac ggttgaggat gcacttgagt tttttgaaaa catcccaaaa
     2161 atcagtcgga agctccagac catcgtcgat gtagggctgg ggtatgtcaa gctcggtcag
     2221 cctgctacga cactatctgg tggggaagca cagcgcgtta aacttgcatc cgaactccac
     2281 cgcagatcaa acgggaagac attctatatt cttgatgagc caacaactgg tctacatgcg
     2341 catgacatct cgaagttgct cgttgttctg caacgactcg ttgaaacagg taacactgtc
     2401 ctcgtcattg aacacaattt ggatgtcatt aagacagtcg atcatgtgat cgaccttggc
     2461 ccagaaggcg gagataaggg tggtcagatt atcgtgacag gtacgccgga agaagtggcg
     2521 gcatgtgaga gttcctatac ggggcaatat ttgaagccga ttttagagag agatcgtcag
     2581 agaatgaaag acatggtgct tgcagcagag tcatcggagt aatgaaacct ttcctagtgc
     2641 tttccgtata tatacgtaag taatgtgcga atcaaaatcg caatttagga ggcggaagta
     2701 tgcaaaacga acggaaacga attttgacga tgcttgagaa cgggacaatc acaacagaag
     2761 aggcgttaac acttctagaa aaactgggtg aatcaaaacg ggagacatct caaactagca
     2821 atacgagcga gagtaacaca gggacaacag accaaggcgg tacacaggac acgactagta
     2881 atcaacgtca ggaatcaaaa gggtttggaa agcaatcgtc aatggatgag ttcttgcaag
     2941 atctgcaacg ggatttctct caagttggcg atcgtttcat gcagtttatg caaacagctg
     3001 tcgagaaagt aaagaccttt gattttgact ctcctcttgg aacaagcgta tcgtttcacg
     3061 aagaaagcac gaaggacgcg acaggtgtca cagaagtggt tgtcgatatt gataatggta
     3121 aattaaacat cataccgacg gaagcggaag aagtccgtgc tgcattttct gtccgggcat
     3181 tcagagcgga ttcagaggaa gctgcacgga aggagtttgc ggataaagtc ctcttcgtga
     3241 atgatggcaa caagcttcgg attgtcagcc atttgaaaat ggtgcaagta aacgtagacc
     3301 tgtatattcc gaatcgagaa tatgaaaagc tttccgtccg tctattaaat ggtgcattca
     3361 agacacgcga gatgaacgtt cgagacattc aagtaaaaac ggcgaacgga aaagttgatg
     3421 tagcagacac gactttcaag accgcagata tcgaaacagt aaatggagcg attcagctca
     3481 atggccttga aggagaaaca ctagaggcag aaacattgaa cggccgcgtc tacattgatg
     3541 gcaaactaaa agacgtggaa gcccaatcgc tgaatggtca tgttgtagta acaacaacag
     3601 acgttgatgc acgaaaagtt gaagcgaaga cagtaacagg tgcagttgaa ctttatgttc
     3661 cgaaagaggt tcctttatcc ggagaaatct ctacgaacat gggtcgactt gatttacagc
     3721 tgactgatgt cgaacgtacg tcagagcaag agcagatttt ccaacgaact cttcgtttta
     3781 aaaaggatgt tgaaggtggg caaactcctc tccacatttt tggagaagct cgtacaggat
     3841 ccgtacttgt ttgctatacc gctgaataaa agaacaaaga taggcccttt cccgagttct
     3901 ttgggaaagg gcttttggta tgcgctacag ttcaaggtct ttcattcgat atacccaagc
     3961 aataatagcg aatgctatcg cataggcaca ttgttcctgg tagtggactt gcttcagcag
     4021 ttccgcctct tttttatttg tcatgaatcc acattccacg aggatggcag gcatgttggt
     4081 atcccgtaaa acggcgaagt ttgcttgttt cacgccacga tctttccgtc cggttgccat
     4141 aattagcgag cgctgcacga gctcggccag agacgttgtt cgagataacg cgcctggata
     4201 tgcataggtt tcgatccctt ccgcgtgatt ccacgtctct ccatgtgcat ttgcgtgaat
     4261 ggacacaaat gcatctacat tcagctgatt cgcacggttt gttctctcga gtaaaggaac
     4321 gtccatctca ccgggatgtg aaaaaagaac cgtaatacct gcacgctcaa gtttcggctt
     4381 aattcgatct gcaacagcca cattaaactg atactcacgc agagtgccat ccggcgatcg
     4441 tttgcctggc gtgtttggcc catgcccagc gtctaccata atttttatca ttctaccccc
     4501 tcctgccatt tacatagaaa aatgacgagg agaagttgac aaatgaaagg aaaaacattt
     4561 cattctctat ctgtcatggt aaaatggtgg atagagtttg aattatgttg gaaagaggga
     4621 tggccatggg aaaagtaac
//
LOCUS       DASULI010000414         3063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1412146,
            whole genome shotgun sequence.
ACCESSION   DASULI010000414 DASULI010000000
VERSION     DASULI010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412146"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="VFX34_08975"
     CDS             complement(<1..1622)
                     /locus_tag="VFX34_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEX5565081.1"
                     /translation="MNIVLTTLNAKYIHTNLAIRYLKSYAMPEYEPILAEYTIKDPSL
                     NIVSDIYQKKPNIVGFSLYIWNIEESIKVIRILKKTNPELVIVAGGPEVTYDFDKWLE
                     EVPEIDFIVIGEGERTFKQLLDYHSGKIGLNEVQGVAYMEEGKLRSTAPGPKLDLREL
                     PSPFRFPEDVPHLGNRVTYIETSRGCPFSCQFCLSSIEVGVRYFNRDSVKEDIRYLMK
                     NGAKTIKFVDRTFNISRSYAMEMFQFLIDEHLPGTVFQFEITGDIMRPEVINFLNENA
                     PAGLFRFEIGVQSTNDLTNELVKRRQNFEKLSRTVTMVKDGGKIAQHLDLIAGLPEED
                     YDSFRNTFNDVFAMRPEELQLGFLKLLRGTGLRIQAEQYGYTYIDEAPYEILSNNVLT
                     FDDILRIKQTEDVLEKYWNDNRMPRTVEYLVSEVFETPFDFFQQFGTFWETKGWSRIG
                     HQLQDLYTRLEEFLLEYGKADMKIVSSLMKIDYLAHHTFQPRHIWWTDDMEPEALAAI
                     ERSVLEDKELLEKMATPTRLTERNIRKHTFITP"
     gene            1828..>3063
                     /locus_tag="VFX34_08980"
     CDS             1828..>3063
                     /locus_tag="VFX34_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5565082.1"
                     /translation="MSDFDKIRKSVSFLMDDNFRPGLALQLNGDIYSENEPFKIHFDM
                     KNKKNLNEIIEAASMLDWTRFSERAEKSDHVLMDLMQVSFLGKEKSPVPMHVFYIPEI
                     KMIVAMFDVPDLKKIPSLKTYYNAFKHSANFMLLVDPLGEIINVNEMHTVFINLTRDE
                     LIGHSIEKVFAIIDPDNSEPLQHHLAKVKEHGCVQSITTYQRSPDDTRYYHITTYYDN
                     DTDMFIVNLSDATEKENLQVQLAHSGSLSAVGQIAASIAHEIRNPITTLKGFTQLLKV
                     SATEDSIRYISVIEDEIERMENILGEMLVLSKPSEKKKSIFSLEVLIGDMVSILSPKA
                     VMEGIEVVQQAELSSNPLIFGDPDKMKQVLLNLFKNALESMQVGGKLSIHLSESDEKL
                     VLSITDTGKGMTRHQVSQVF"
BASE COUNT          869 a          657 c          645 g          892 t
ORIGIN      
        1 ggggtgatga atgtatgctt ccggatattc ctttcagtga gccgagtcgg cgtcgccatt
       61 ttttcaagca gctctttgtc ttccaaaact gaacgttcga ttgctgccaa tgcttcaggc
      121 tccatatcgt cagtccacca aatatgacgc ggttggaatg tatgatgggc cagataatcg
      181 attttcatta gactgctgac gattttcata tccgcttttc cgtattcgag aaggaactct
      241 tccaacctcg tgtataagtc ttgaagctga tggccgattc tggaccagcc tttcgtttcc
      301 caaaatgtgc cgaattgctg gaagaagtcg aaaggcgttt cgaacacttc cgatacaaga
      361 tattcaaccg tccgcggcat ccgattatca ttccaatact tctcaaggac atcctccgtc
      421 tgcttaatcc gtaaaatatc gtcgaatgtg aggacgttgt tcgatagtat ttcatatggc
      481 gcctcatcga tatacgtata gccgtattgc tcagcctgga tgcgcaagcc ggtccccctc
      541 aatagtttga gaaagcctaa ttgcagctcc tcaggcctca tcgcgaatac atcattgaat
      601 gtatttctga atgaatcata atcctcttcc ggcagccctg ctatcaaatc aagatgctga
      661 gcgattttcc cgccatcctt caccatagtg acggtcctgg acagcttttc gaaattctgt
      721 ctccgtttaa caagttcatt ggtcaaatca tttgtcgatt ggacaccgat ttcaaatcgg
      781 aagaggccgg caggggcatt ctcattcaag aagttgatga cttccggacg catgatatcg
      841 ccggttattt cgaattggaa cacggttccg ggcaaatgtt catcgatcag gaattgaaac
      901 atctccatcg cataactacg gctgatattg aatgtgcggt cgacaaattt gattgtcttt
      961 gcaccatttt tcatcaagta acggatgtct tccttcactg agtcccgatt gaagtacctg
     1021 acgccgactt cgatggaaga taaacagaac tgacaagaga acggacagcc acggcttgtc
     1081 tcaatatacg tcacccggtt gccgaggtgg gggacatctt cagggaatct gaaaggggag
     1141 ggaagctctc gcaagtccag ctttggcccc ggagccgtcg atcgtaactt tccttcctcc
     1201 atatacgcga cgccttgcac ttcgttgagg ccgattttcc ccgaatggta gtccagcagt
     1261 tgtttgaaag tcctttcccc ttcaccgatc acgatgaaat cgatttcagg gacttcttcc
     1321 aaccatttgt caaagtcata tgtcacttca ggtcccccag caacaatgac cagttccgga
     1381 ttcgttttct tcaagatcct gatcaccttg atggactctt caatattcca tatgtacaaa
     1441 ctgaatccga caatatttgg ctttttttga taaatatctg atacaatatt caaagacgga
     1501 tctttaattg tatattccgc caatatcggt tcgtattctg gcatcgcata agatttcaaa
     1561 tatcggatgg ccagattcgt gtgaatatat tttgcattta atgttgtcag gactatattc
     1621 atattctttc tcctttggta taatgctaat tacctgttag aatgatctgc attttagtta
     1681 cttcatacta ttttattgcg actgataata tgaaataatc agaattgaag atgctttatt
     1741 aaagtgaagt gcaattgaaa tggttgcgtc cgcatgaaat cttgtcgttt cacatactgt
     1801 ttggtaatag gcacaggagg tatcaaattg agtgatttcg ataaaataag aaaaagcgtg
     1861 tcattcttaa tggatgataa ctttcgacca ggtttggctt tacagttgaa tggagacatt
     1921 tattctgaaa acgaaccatt taaaatacat ttcgacatga aaaacaaaaa gaatctcaat
     1981 gaaatcatcg aagcggcttc catgcttgac tggacaagat tttctgagcg ggcagaaaaa
     2041 tccgatcatg ttttgatgga tcttatgcaa gttagttttc ttggtaaaga gaaaagccca
     2101 gtcccgatgc atgtattcta tataccggaa attaagatga ttgtggcgat gttcgatgtg
     2161 ccggatctta aaaaaatccc ttccctcaaa acttattata atgcattcaa acactctgcc
     2221 aattttatgt tgttagtgga tccgctcgga gaaatcatca atgtgaatga gatgcataca
     2281 gtgtttatca atctaacgag ggatgaattg atcggtcata gcatcgaaaa ggtgtttgct
     2341 attatcgacc cggacaatag tgaaccgctt caacatcacc ttgccaaagt gaaggagcat
     2401 ggctgtgtcc aatccataac aacgtatcaa agatctccgg acgatacaag atattatcat
     2461 ataacaacct actatgacaa tgatactgat atgtttattg ttaatttgtc agatgccacc
     2521 gaaaaagaga acctccaagt gcaattggcg cattcaggtt cattatccgc tgtcggacaa
     2581 attgcggcaa gcattgccca tgaaattaga aacccgatta caacattgaa gggatttacc
     2641 caactgttga aagtatcggc taccgaggat tcaatccgct atatctctgt tatcgaggat
     2701 gaaatcgaaa gaatggaaaa catcctaggg gaaatgcttg tcctctcaaa gccatccgaa
     2761 aagaagaaat ccatcttctc gttggaagtt ttgatcgggg acatggtttc aatactatct
     2821 ccaaaggcgg tcatggaggg aatagaagtt gttcagcagg cggaattgag cagtaatcca
     2881 ctcattttcg gcgatccgga taaaatgaag caagtacttt tgaatttatt taaaaatgca
     2941 ttggagtcga tgcaggtcgg ggggaagctt tccatccatc tgtcagaatc tgacgaaaaa
     3001 ctggtcctct ccattacaga tacgggaaaa gggatgactc gccatcaagt gagccaagtg
     3061 ttc
//
LOCUS       DASULI010000415         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_461992, whole
            genome shotgun sequence.
ACCESSION   DASULI010000415 DASULI010000000
VERSION     DASULI010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461992"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFX34_08985"
     CDS             complement(<1..212)
                     /locus_tag="VFX34_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEX5565083.1"
                     /translation="MFLHQLNPSMKALTILLLVVLLALIFDPVTPSLLLLCTILLTFL
                     GGNVDWRKYFLYFLPFALLSFGMLWTT"
     gene            complement(193..1863)
                     /locus_tag="VFX34_08990"
     CDS             complement(193..1863)
                     /locus_tag="VFX34_08990"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5565084.1"
                     /translation="MTPVVTVKDFSLRFEFAERDTLSGVSFSLERAESILVLGPSGCG
                     KSTLTLCLNGLYPRELDGSMSGEILINGKPTTTYEPGQLSRHVGVVFQDPETQFCMLT
                     VEDEVAFGLENIGVPHEEMEDRIDQALQLVNMQEFKQAMIATLSGGQKQKLALACILA
                     LEPEVLILDEPTANLDPIARIDVIQTIKRLKDELGLTMLIIEHQLDQWTDLADRCLLF
                     NQTGTIFFDGSLREAIRTHLPKIKQEGIWLPNVAQYVLPYVDPATRAIPLSLEEFACQ
                     AHVWNGIEWNQRERNGIQTKEKVSLETKNVSFSVGEKPILRNISLTLHENEFVAIVGA
                     NGSGKTTLSRVLAGLQKPTKGSVTLHGKPLHSWREADLRNEIGYVFQNPEHQFITNTV
                     YEEVAFSLQIKGVPENEVHERVTQILERYRLAAYVDEHPYTLSQGQKRRLSLATMLVD
                     NQKMLLLDEPTFGQDAATNLEMMNMLQDRFEVGTTIVMITHDMELVHKYVDRIIVLNK
                     GTVVADCTPEELWDMPDQKLYEWGLDLPIPVKMQRYCEKEDHYVLTST"
     gene            complement(1860..>2421)
                     /locus_tag="VFX34_08995"
     CDS             complement(1860..>2421)
                     /locus_tag="VFX34_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015383481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX5565085.1"
                     /translation="KEIVVLSALSVVFAIVYLAFLPLGKLLVGFFGPIGYDLIFGIWF
                     IVSIIAAYIIRKPGAAFLSETIAAMVEVLIGNAVGPMLIVVGMVQGIGAEAVFAATRW
                     RLYSTWVLMLAGVGSAVTSFIYGFFMSGYAALGTGYVTAMFVTRMISGALIAGLLGKA
                     ISDGLAKTGVLSSYALGKEVRKRKMA"
BASE COUNT          647 a          613 c          525 g          636 t
ORIGIN      
        1 gtcgtccaca gcataccgaa ggacaataat gcaaaaggca agaaataaag aaaatacttc
       61 ctccagtcta cgttacctcc gagaaacgtc agcagtatgg tgcatagcaa caagagggag
      121 ggggtcaccg ggtcaaagat cagagccaat agcacgacaa gaagtaaaat cgtcaacgct
      181 ttcatacttg ggttaagttg atgtaagaac atagtgatct tccttttcgc aatatcgttg
      241 catttttaca ggaatcggca agtccaatcc ccactcatat agcttttgat ccggcatatc
      301 ccataactct tctggagtgc aatccgctac gacggtcccc ttgttcaaga caatgattcg
      361 gtctacgtac ttgtgtacca gctccatatc atgtgtaatc atgacaattg tcgttcctac
      421 ttcaaaccga tcctgaagca tgttcatcat ctcgaggttt gtcgcagcat cttgcccgaa
      481 tgtcggctcg tctaatagaa gcattttttg gttgtccacc agcatggttg caagacttag
      541 tcgcctcttt tgtccttggc tcagcgtata cggatgttca tccacataag cagccaatct
      601 gtaacgttcc aggatttgag tgacgcgctc atgaacctcg ttttccggca ccccttttat
      661 ttgaagactg aatgcaactt cttcatacac agtatttgta ataaattgat gctcaggatt
      721 ttgaaataca tacccgattt cattgcgcag atcagcctct cgccagctgt ggagcggttt
      781 cccatgtaag gtgacagacc ctttcgtagg cttttgtaag ccggccagta cgcgcgaaag
      841 ggtagttttc ccgctgccgt ttgccccgac gattgctaca aattcgttct catgaagcgt
      901 gagggagata tttctcaaaa tgggtttctc tccaactgaa aaggaaacgt tctttgtttc
      961 aagggatacc ttttctttag tctgaattcc gttccgttcc ctttgattcc actcgattcc
     1021 attccaaaca tgagcttggc atgcgaactc ttctaacgaa aggggaattg cccgggtcgc
     1081 aggatccacg taaggcaata catattgtgc tacgttcggc agccagattc cttcttgctt
     1141 aattttcggt aggtgcgtcc gaattgcctc tcgtagcgaa ccatcgaaaa agatggtacc
     1201 ggtttgattg aataaaagac atcgatctgc taagtctgtc cattggtcca gttgatgctc
     1261 aataatgagc atggttaagc cgagttcatc ctttaaccgt ttaatcgttt ggatgacatc
     1321 gattctagca ataggatcta gatttgctgt tggctcatcc aggatcaata cttcgggttc
     1381 gagcgcaaga atgcaggcga gtgcaagctt ttgcttttgc ccaccggata aggtagcgat
     1441 catggcttgt ttaaactcct gcatattaac gagttgcagg gcttggtcaa tcctgtcctc
     1501 catctcctca tgaggaaccc ctatgttttc tagtccgaac gcaacttcat cctcaacggt
     1561 cagcatgcaa aactgcgtct caggatcttg aaagacaacg ccgacatgtc ggctaagctg
     1621 tcccggttca taggtagttg ttggctttcc gttaatcaaa atctctccgg acatcgagcc
     1681 atccagttca cgtggataga gtccattcag acaaagggtc agtgtactct ttccacatcc
     1741 acttggaccg agtacaagaa tgctttcagc gcgttcgagt gagaaggaaa cgcccgacaa
     1801 ggtatccctc tctgcgaatt cgaatcgcaa agagaaatcc tttactgtga caacaggcgt
     1861 catgccatct tcctctttcg cacttctttc ccgagcgcat agctcgaaag tacccctgtt
     1921 ttagcgagtc catcactaat cgcttttccg agaagtccag cgattaaggc accgctaatc
     1981 atacgggtga caaacattgc cgtcacgtag ccagttccaa gtgctgcata cccgctcata
     2041 aagaagccgt agataaagct cgtaactgca gacccaaccc ctgcaagcat cagaacccac
     2101 gtggaataaa gtctccatct cgtggcggca aagaccgctt ctgcaccaat tccttgtacc
     2161 ataccgacta caatgagcat cgggccgact gcattcccga tcagcacctc aaccatggcg
     2221 gcaatcgttt cggacaagaa ggctgcaccc ggcttccgaa tgatataggc agcaatgatg
     2281 gaaacaataa accaaattcc aaatatcaaa tcatagccga tcggaccgaa aaatccgaca
     2341 agcaattttc cgagaggcag gaacgccaag tagacgattg caaagacgac ggataaagcg
     2401 gagagaacaa caatctcttt a
//
LOCUS       DASULI010000416         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1414441,
            whole genome shotgun sequence.
ACCESSION   DASULI010000416 DASULI010000000
VERSION     DASULI010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414441"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFX34_09000"
     CDS             complement(<1..260)
                     /locus_tag="VFX34_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019381136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX5565086.1"
                     /translation="MNTTVKVRQKEKTVKRSIKETTNRMMGIGTDDAKLAKSYMSVAF
                     LALLIGGVLGLLQGLERAGLMQLPHWFNYYQVLTAHGVLLVLV"
     gene            complement(275..742)
                     /locus_tag="VFX34_09005"
     CDS             complement(275..742)
                     /locus_tag="VFX34_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5565087.1"
                     /translation="MKMNRYEEIWLLLSGGMLVIFMLITGYQTFALEMGPPSNKETID
                     PQKVDQTAPFDKPGVYQVGENEYEVVMTLEVFNFNPGNIEIPVGSTVTFIMTSKDVVH
                     GFQVADTNLNAMVMPGHIQKTTQTFNKPGEYLVLCNEYCGAGHQHMSTTITVK"
     gene            complement(767..916)
                     /locus_tag="VFX34_09010"
     CDS             complement(767..916)
                     /locus_tag="VFX34_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017796861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 2A"
                     /protein_id="HEX5565088.1"
                     /translation="MKLHEAPLRNNQEQEDENTVKGTMASVGIVGLVILVMWVLLFAI
                     YMYRF"
     gene            complement(1100..1795)
                     /locus_tag="VFX34_09015"
     CDS             complement(1100..1795)
                     /locus_tag="VFX34_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX5565089.1"
                     /translation="MDSVIGFLKNTMFFKDLSDRELEPFLPIMNRRKLADKTMLFLQG
                     DPITHIFIATAGKIKIFRNDQAGKEQVLHFKQHGDLFPHVGFFRQGHYPAHAQAIEDS
                     EVYSMSIKDFEQVLMANPSLSIKLFRMLGDQIVSLQQRLEELALRSANERILLLLLRL
                     CETHGSAVGNEGWVKLNTRFKNADLANMIGTSRESVNRMISQLRKDDAVQLKEGEYLI
                     RPEILKDELLNFN"
     gene            complement(1930..>2159)
                     /locus_tag="VFX34_09020"
     CDS             complement(1930..>2159)
                     /locus_tag="VFX34_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5565090.1"
                     /translation="ADELYKNPLHPYTKSLLSAIPLPDPIYERSRVRQTYDPKTHNYG
                     PDEKVEFREVTPGHFVLCSEKEFKELKAQVK"
BASE COUNT          563 a          515 c          428 g          653 t
ORIGIN      
        1 acaagcacga gcaatacacc gtgggcggtc aacacttggt agtagttgaa ccaatgtggc
       61 agctgcatga gaccagccct ttccaaacct tgcaacaatc ctaatactcc accgatgagc
      121 aaagcaagaa atgcaacact catataagat tttgcaagtt tggcatcgtc agtcccgatc
      181 cccatcattc tattcgttgt ttccttgatg gaccgcttta ctgtcttttc tttttgacga
      241 accttaactg tcgtattcat aatactcccc tccttcattt cacagtgatt gttgtgctca
      301 tatgctgatg gcccgcgccg cagtattcat tgcacaacac aaggtattct ccaggtttat
      361 tgaatgtctg cgtcgttttt tgaatatggc cgggcatcac catcgcattc aaattcgtgt
      421 ctgcgacttg gaatccatgg acaacatcct ttgaagtcat aatgaacgta actgtcgagc
      481 caaccggaat ttcaatgttg ccgggattga aattgaacac ttccaacgtc atgacaactt
      541 catattcatt ctcacctacc tgataaacgc ctggcttatc gaatggagcc gtctgatcca
      601 ccttttgcgg atcgattgtt tctttattgc ttggaggtcc catttctagc gcaaatgtct
      661 gataacccgt aatgagcatg aaaatgacta acattccgcc gctcaatagt aaccagatct
      721 cttcatagcg attcattttc atcgttcttc ctcctaatct tcttctttag aaacgataca
      781 tataaatggc gaacagcaat acccacatca ccaagatgac aagacctaca atgcccacgg
      841 aagccattgt ccctttgaca gtgttttcat cttcctgctc ttgattgttt cttagtggcg
      901 cttcatgaag tttcattcgg aatcacctcc actgctttgt tgatgtcttc ataatagttg
      961 ataacagttc tcaccaatgt gatgtacatc acatttccaa aacgtgaaag tgacaaaacg
     1021 gtgacattga gcccaacgaa aaagccattt tacagatcca ataaattgga ctgcaaaatg
     1081 gcttaaaaca gcttttgttt cagttaaagt tcagcaattc gtctttcagt atttcaggac
     1141 ggatcaagta ttccccttct ttcaattgca cggcatcatc cttgcgaagc tgcgagatca
     1201 tccgattgac agactccctc gacgttccga tcatattggc aagatccgcg tttttgaaac
     1261 gggtattcaa cttcacccat ccttcatttc cgacagcaga gccatgcgtt tcgcatagcc
     1321 ggagcaatag cagcaaaatc cgctcgttcg cactgcgcaa tgccagctct tccaatcgtt
     1381 gctgcaagct gacaatctga tctcctagca tcctgaatag cttgatggat aaggatggat
     1441 tcgccatgag aacttgctcg aagtctttaa tcgacatgga atatacttca ctatcttcta
     1501 ttgcctgtgc atgtgccgga taatgccctt gacggaaaaa gccgacgtgc ggaaacaggt
     1561 ccccatgctg tttgaaatga aggacctgct cttttcctgc ctggtcattc cggaaaatct
     1621 ttatttttcc cgccgttgca atgaatatat gggtgatcgg atcgccttgc aaaaatagca
     1681 tcgtcttatc ggcaagcttc cgacggttca tgatcgggag gaaaggctcc aattcccggt
     1741 cggataaatc tttaaaaaac attgtgtttt tcagaaatcc gattacagaa tccattgtat
     1801 ctccccttct aatgaaagtt ggcgattata ctactctata ttgtagcatt cttatgtatg
     1861 gaacgggcta gaaatagaaa accgccaggc gatcgcttta tccgatgcca ttggcggttt
     1921 ttttattcat catttaactt gtgccttcaa ttctttgaac tctttttcgg agcaaagtac
     1981 aaagtggcca ggtgtgactt cgcggaattc cactttttca tcaggtccgt agttatgcgt
     2041 tttcgggtca taggtttgtc gaacccgtga tcgttcataa atcggatctg gaagcggaat
     2101 tgcggatagc aatgattttg tgtaagggtg caatggattt ttgtataatt catcagcgg
//
LOCUS       DASULI010000417         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_112289, whole
            genome shotgun sequence.
ACCESSION   DASULI010000417 DASULI010000000
VERSION     DASULI010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112289"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFX34_09025"
     CDS             <1..188
                     /locus_tag="VFX34_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase"
                     /protein_id="HEX5565091.1"
                     /translation="LYLYMAPKLIGNGPTIFMEESRKLMADAESLHILDVRQIGPDIL
                     IHASFNKGGDAIVHRNH"
     gene            169..819
                     /gene="ribE"
                     /locus_tag="VFX34_09030"
     CDS             169..819
                     /gene="ribE"
                     /locus_tag="VFX34_09030"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018393420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5565092.1"
                     /translation="MFTGIIEEIGIVRGIHAGQNSMRLTIECKKVLEDVRKGDSIAVN
                     GVCLTVSNYTSSQFVADVMPETVKATTLYELKAGSPVNLERAMAANGRFGGHFVSGHV
                     DGVGEVVSIRKHENAIYMDIALATELLNYVIPKGSIAIDGTSLTVFDVTDDTVTISLI
                     PVTQQDSILGQKNRGALVNVECDMLAKYIERFTGRQAMTPKRGVTMETLAANGFLT"
     gene            833..2038
                     /locus_tag="VFX34_09035"
     CDS             833..2038
                     /locus_tag="VFX34_09035"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX5565093.1"
                     /translation="MYTTVEHAIEELKKGKAIIVVDDENRENEGDFVALAEFATPDMI
                     NFMASEGRGLICVPIEERKAEQLNLTPMTYQNTDVHGTAFTISIDHVNVHTGISAFER
                     SETIMKMLEPTAVPSDFRRPGHIFPLVAQPGGVLIRPGHTEAAVDLAKLAGSAPAGVI
                     CEIMNADGTMARGEQLAEIAKRFDLCILTIKELIAYRKKKERLVERVVDIQLPTSFGE
                     FKACAYVETLTGREHVALVKGSIEEMEAPLVRIHSECLTGDVFGSLRCDCGPQLHAAL
                     EQIEEAGAGILLYLRQEGRGIGLINKLKAYKLQEQGYDTVEANEQLGFADDLREYGIG
                     AQMLEDLGVTRIQLLTNNPRKISGLSDYGIDIVDRIQIELPVVEENRHYMQTKKTKLG
                     HLLSHGGGI"
BASE COUNT          612 a          424 c          542 g          506 t
ORIGIN      
        1 agctttattt gtacatggcg ccaaagttga ttggcaatgg tccgaccatt tttatggagg
       61 aatcgcgaaa gttgatggcg gatgcagaat cccttcacat acttgacgtt cgtcaaattg
      121 gtccggatat actgatccat gcaagcttca acaagggagg cgacgcaatt gttcaccgga
      181 atcattgaag aaatcggtat cgtgcgtggc atacatgcag gccagaattc catgcgcctc
      241 acgattgaat gcaagaaagt gttagaggac gttcggaaag gcgatagcat cgcggtgaat
      301 ggtgtttgcc tgaccgtatc caactacacg tcatcccaat tcgtggctga tgttatgcca
      361 gagactgtaa aagcgacgac gttatatgaa ttaaaagcag gtagtcctgt gaacctggaa
      421 cgggcgatgg ctgcaaatgg ccgcttcgga ggtcattttg tcagtggtca tgtggatggt
      481 gttggcgaag tcgtctccat tcggaaacat gaaaatgcaa tctacatgga cattgcactt
      541 gccacggagc ttttgaatta tgtcatcccc aaagggtcga ttgcaatcga tggaacatcg
      601 ctcacagtat ttgatgtaac ggatgatacc gtcacaattt cattgattcc tgtcacacag
      661 caggattcca tacttgggca aaaaaatcga ggcgctctcg taaatgtcga gtgtgacatg
      721 ctcgcaaaat acattgaacg atttactgga agacaggcta tgaccccaaa acggggagtt
      781 acgatggaaa cacttgctgc caatggattt ctaacataag gatgcgaatg atatgtatac
      841 aactgtagaa catgcaattg aggaattaaa aaaaggaaaa gcgattattg tcgtggatga
      901 tgaaaatcgg gaaaacgaag gggattttgt tgcgcttgcc gaatttgcca cgcctgacat
      961 gattaatttt atggcgtcag aagggcgagg cttaatctgc gtcccgattg aggagaggaa
     1021 ggcagagcag ctcaacttaa cgccaatgac gtaccaaaat accgatgttc atggaacagc
     1081 ttttacaatt agcatcgacc atgtgaacgt ccatacaggc atttctgcgt ttgagcggtc
     1141 ggaaacaatt atgaaaatgc ttgagccaac cgcagtacca tccgattttc gaagaccagg
     1201 ccatatcttc ccattggttg cacagccagg tggggtgtta attcgcccgg ggcatacaga
     1261 ggccgctgtt gatttagcga agcttgctgg ttctgcacct gcaggtgtca tctgtgaaat
     1321 tatgaatgcg gatggaacaa tggcaagagg agagcaactt gccgaaatcg cgaagcgttt
     1381 tgatctatgc attctaacaa taaaagaatt gattgcctac cggaaaaaga aagaacggct
     1441 tgtggaacga gttgtcgata tccaactgcc aacctcattc ggagaattta aagcctgcgc
     1501 ttacgtggaa acattgactg ggcgcgagca tgtggcattg gtgaaaggat caattgaaga
     1561 gatggaagcc cctctcgtcc ggattcattc ggaatgctta accggcgacg tctttggttc
     1621 attgcgatgt gattgtggac cgcagctgca tgcggcactt gagcagattg aggaagctgg
     1681 agcgggcatt ctgttgtatc tccgccaaga gggccgtgga atcggactca tcaataaatt
     1741 aaaagcctac aaactgcagg aacaaggcta tgacacagtc gaagcgaacg aacaattagg
     1801 gttcgcggac gatttgcgtg aatacggaat cggtgcgcaa atgttagagg atttgggtgt
     1861 gacacgaatc caattgctca caaacaaccc ccgtaagatt tcgggtttat ccgactatgg
     1921 cattgacatt gtcgaccgga ttcaaatcga gctgcctgtt gttgaggaaa atcggcacta
     1981 tatgcaaaca aagaaaacaa agctcggaca cctattgagc catggaggag gaatatgatg
     2041 aaaacaacat acgaaggaca tttaatagga acaggtttaa aaat
//
LOCUS       DASULI010000418         5944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_167004, whole
            genome shotgun sequence.
ACCESSION   DASULI010000418 DASULI010000000
VERSION     DASULI010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167004"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VFX34_09040"
     CDS             <1..175
                     /locus_tag="VFX34_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017472338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX5565094.1"
                     /translation="AVVAAVLNGYIEKGIDVTVHLSGGDLRIKWADDDHVWMTGPAET
                     TIQGVFHWSRQDS"
     gene            205..1434
                     /locus_tag="VFX34_09045"
     CDS             205..1434
                     /locus_tag="VFX34_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX5565095.1"
                     /translation="MAKKLKAYRFPLILLASIIIGSIIGLIFGEKASVIKPLGDLFLN
                     LLFMIVVPLVFFSISSAVANMDSMKRLGKIMGSMLSVFIITGIIASLTMLVAVLIFPV
                     GSGVDIPMEIIEATEMLSLSDQLVNTFTVPDFVNLFSRKHMLALIVFSVLLGIATGLT
                     GEAGKPFARFLESGSEIFMKVIQLVMYYAPIGFGAYFAALIGAFGAELIGDYAQAFIL
                     YYPVAFLYFLVGFTLYAFIAGGKKGVVRFWKNILAPAATALGTGSSFATLPINLQAAK
                     QIGVPKDIRETVLPLGATIHMDGSCLSAILKIAFVFGVFNMDFTGFGTFVTAIGISLL
                     SGVVMSGIPGGGFIGEMVIVTLYGLPIQALPMISAIGVVVDPPATMVNATGDTVASMI
                     VTRQLEGKDWMDKVNLE"
     gene            1788..2804
                     /locus_tag="VFX34_09050"
     CDS             1788..2804
                     /locus_tag="VFX34_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX5565096.1"
                     /translation="MKKCFKFLLAVVMVLSGLYLFPTGGASAEGKEVGGTYYIDVTAA
                     TIWTAPNLLREVDKYSATNPVDLRKWTTSMTYQDKLGLVGKLETQALYGAKVTVLENR
                     GDWVKVAVHTHPTPKSEVGYPGWMPAVQLAKDKSFENQLHRPFALVTSPTAWLYNEKG
                     AKNQFLELSFNTRLPVMQEFENEVLVATPSDGKKWLKKSDVAVHNHEQDIPSPTGKAI
                     VETGMKFLNLPYLWAGTSGFGFDCSGFTHTIYHANGIVIPRDSSVQATHGTPVERKDL
                     QAGDLIFFAYQEGKGRVHHVGVYIGDGKMLHSPNSSTHVRIDAIDASGYGKEYAGARR
                     YVKK"
     gene            3212..4402
                     /gene="gltS"
                     /locus_tag="VFX34_09055"
     CDS             3212..4402
                     /gene="gltS"
                     /locus_tag="VFX34_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEX5565097.1"
                     /translation="MIELNQITTLFLAAALYLLGTALVNRIGILDRFLIPAPVIGGLL
                     FAIVALILKSSGIVEVSLDTSLQSLFMVAFFTTVGLGASFKLIKLGGKLLIIYLLLCG
                     VTVFMQNFIGVSLAKVFNIDPLLGVMSGAVTMSGGHGGAAAYGTTIEGLGVTSAVTIG
                     MAAATLGLICGSLSGGPIARHLIGKYNLKPKEGVVKEFVDEDEKPIGQHSFMIQVTLI
                     TLAMACGIVLGDLFTEATGFVLPGYIGAMFFAVLIRNIMDRFLPGAINMNEINLIGDV
                     SLAIFLSMALMSIKLWELAGLALPLVGIVFVQVVFIVGFAVFIMFRALGKDYDAAVMI
                     SGFLGHGLGATPNAMANMGATVNKFGPSRTAFLVVPIVGAFLMDVVFSAPIIITTINL
                     LAPK"
     gene            4666..5235
                     /locus_tag="VFX34_09060"
     CDS             4666..5235
                     /locus_tag="VFX34_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5565098.1"
                     /translation="MAEVLLLHHVCGRTKGIEAIADQLRDAGHTVHVPDLFDGRVFSS
                     LEEGLVFVKEIGFEEVTARGVRAASELSRDVVYAGFSLGVPAAQQLAQTREGARGALF
                     FHACLPTSAFESEWPADLPVQIHAMNADPFFVEDGDIDAARELVASANHAELFLYEGK
                     EHLFTDSSLPSYDAGATKLVIKRVLDFLA"
BASE COUNT         1588 a         1296 c         1392 g         1668 t
ORIGIN      
        1 ggctgtagtc gctgcggtcc taaacggcta tatcgaaaaa gggattgatg tcacagtcca
       61 tttgagcggc ggtgatttac gcattaaatg ggcagacgat gatcatgttt ggatgaccgg
      121 tccggcggag actacgattc aaggggtctt tcactggagc cgtcaggatt catagataga
      181 tatcattcta aagggggcaa ttgcatggcg aaaaaattga aagcgtaccg ttttccactc
      241 attcttctcg cttccattat catcggttcc attatcgggc ttatttttgg agaaaaggcc
      301 agcgtcatta aaccgcttgg ggatttattc ttaaatttac tgttcatgat tgttgttcca
      361 cttgtgttct tttcgatttc atcggctgtc gcgaatatgg acagcatgaa acgtctagga
      421 aaaatcatgg gctccatgct atcggtattt atcattacag gaattattgc atccctcacg
      481 atgctagtag ccgttcttat tttcccggtc gggtccgggg tggatattcc tatggagatc
      541 atagaagcca cggagatgct ctcgctatcc gatcaactcg tcaatacctt caccgtgccg
      601 gacttcgtaa atttattttc acgtaaacat atgctcgctt taatcgtatt ctcggtgtta
      661 ctaggaatcg cgactgggtt gactggcgaa gccggtaagc cgtttgcccg ctttttggaa
      721 agcggctccg aaatcttcat gaaagtgatt caactcgtca tgtactatgc cccgatcggc
      781 tttggcgctt attttgcagc actaatcggc gcttttgggg cagaacttat tggtgattat
      841 gcgcaagcct tcatcctgta ctaccctgtt gcatttctat acttcctggt tggattcacg
      901 ctttacgcat tcattgccgg cggaaaaaaa ggagtcgtcc gtttttggaa aaacatcctt
      961 gccccagcgg caactgcgct tggtactggc agcagttttg caaccctccc gatcaacttg
     1021 caggcagcga aacagatcgg cgtgccgaag gatatacggg aaaccgtctt gccgctcggc
     1081 gcgaccattc atatggatgg atcttgcctt tctgccatcc tcaaaatcgc attcgtcttc
     1141 ggcgtgttca atatggattt cacaggattc ggcacattcg taacagcgat cgggatttcc
     1201 ttactgtcgg gtgtcgtgat gagtggaatt cccggcggcg gattcatcgg tgaaatggtc
     1261 attgtcacac tttacgggct gccgatccag gcattgccaa tgatttcggc aatcggcgtc
     1321 gttgtcgacc cgcctgcaac catggtcaac gctaccgggg acaccgtcgc aagtatgatt
     1381 gtgacaaggc aattggaagg gaaagactgg atggataaag tgaatttgga atgaagctta
     1441 tttatgaaat agaagagtta actgaggaac gcgtcgagcg ttcctttttt cagttcaccg
     1501 tgggtagata taaagtgatg aaatgaaaat ataggttggt aaaggaacga ttcccgcatg
     1561 aaatgtttga tttatacccg ctatggttcg aggtccgata cggcctttcc tccttatatc
     1621 atttgatttt tgatagtatt cttgttttat aatctgaaag taaacacttg gttgtatatt
     1681 tgctcaatag ttacttttca cctatcaact ttataaggct tggctatata ctggtagtgt
     1741 atgacggatt gaagtgtcac taccaaacta aaagggggat ggttgtaatg aagaaatgct
     1801 tcaagttctt gctcgcagtt gtaatggttt tatcgggttt gtatttattc ccgacaggcg
     1861 gtgcgtcagc ggaaggcaaa gaagtcggcg gtacgtatta tattgatgtt accgcggcaa
     1921 caatttggac agctccgaat ttactaaggg aagtcgacaa gtattccgcg acgaatccgg
     1981 ttgatttacg aaaatggaca acaagcatga cgtatcaaga caaacttggt cttgtcggaa
     2041 aactggaaac tcaagcctta tacggagcga aagtgacagt actcgaaaat agaggcgatt
     2101 gggtgaaggt agctgtccac acgcacccaa cccctaaaag cgaagttgga tatcctggct
     2161 ggatgcccgc agtccaattg gcaaaggata aaagctttga aaatcagttg caccgaccgt
     2221 ttgccctcgt cacttctcct acagcttggt tatataatga aaaaggtgca aagaatcaat
     2281 tccttgagct aagcttcaac accagattac ccgttatgca agaattcgaa aatgaagtgt
     2341 tggtcgctac gccaagtgac ggcaagaagt ggctaaaaaa atcggatgta gctgtccata
     2401 atcacgagca agatatccca tctccaaccg gcaaagctat cgtcgaaacc ggcatgaagt
     2461 tcttgaacct tccttattta tgggcaggga catccggctt cgggtttgat tgctcgggat
     2521 tcacacacac aatctatcat gcaaatggca ttgtcatccc tagggattcc tctgtacagg
     2581 ctacacatgg cacacctgtt gaaaggaaag atcttcaagc cggcgacctt attttcttcg
     2641 cttaccaaga agggaaagga agagtccacc acgtcggggt ctatattggg gacggcaaaa
     2701 tgctgcattc gcccaactca tcaactcacg tacgtattga cgccatcgac gctagtggat
     2761 atggaaaaga gtacgcaggt gcaagaagat acgtgaaaaa ataaataatt attcaagccc
     2821 ctccattgta aaacgggaga ggcgcagtcc cccatccaat ctttgggatg ggggattttt
     2881 atttattcta aaccccttgt ccagcaactc ataaaaggta attttatttt tcagagtcac
     2941 aaaccacagg aatgcggtcg cgatagtgct ggatggattt atttattttt aaagaaaatt
     3001 gccaagacaa ttttatattc ctatactgtt caaagttgcc tacggatttg ttacattttt
     3061 aagataggcc atgcgtgttt acttccgaaa cgggtatcgt acacagataa ggcccattga
     3121 ttcgtcccaa aacagaaata ctgtggccag tataaaggag agggatcgag catcacatcg
     3181 tcctctcgac gtaaaggaaa ggtgaaattg catgattgaa cttaaccaga tcacaacatt
     3241 gtttctagct gcagcactgt acttgctcgg cacggcgctt gtcaaccgga tcggcatcct
     3301 tgaccggttc ctcattcctg cacccgtcat cggcggtctg ctgttcgcga tagtcgcact
     3361 catcttgaaa tcgtccggca ttgtggaagt ttcattagat acatccttgc aatccttgtt
     3421 catggtcgcc ttcttcacga cagttggact cggcgcaagt tttaagctga tcaagcttgg
     3481 cggcaagctc ttgatcatct atttgctgct gtgcggcgta accgttttca tgcagaactt
     3541 cattggcgtc tcccttgcga aagtgttcaa tatcgatccg ctgcttggcg tcatgagcgg
     3601 cgccgtgacg atgagcggtg gacacggtgg agcggctgca tacgggacga cgatcgaagg
     3661 tctcggcgtc acatctgccg tgacgatcgg tatggccgca gcaacactcg gattgatctg
     3721 cggaagcctg tccggcggtc cgatcgctcg tcatttaatt ggaaagtata atctgaagcc
     3781 gaaagaaggc gtcgtgaaag agtttgtaga tgaggatgag aagccgatcg gccaacattc
     3841 gttcatgatt caagtgacgc tgatcacgtt agcaatggca tgcggaatag tccttgggga
     3901 cctgttcacc gaagcgaccg gcttcgtctt gcccggctac atcggcgcga tgttctttgc
     3961 ggtactcatt cgcaacatca tggaccgttt cttgccggga gctatcaaca tgaatgaaat
     4021 caacctgatc ggagacgtgt cgcttgccat cttcctgtcc atggcactta tgagcatcaa
     4081 actatgggaa ctcgcgggac tcgcgcttcc actcgtcggc attgtcttcg ttcaagtggt
     4141 cttcattgtc ggatttgccg tgttcatcat gttccgtgcg ctcggcaaag attacgacgc
     4201 agcggtcatg atttccggct tccttggcca cggactcggc gcgacaccaa acgcgatggc
     4261 caatatgggc gccaccgtga ataaattcgg cccatcacgc acagcattcc ttgtcgtccc
     4321 gatcgtcggc gcatttctga tggacgtcgt cttcagcgca ccgatcatca tcacaacaat
     4381 taacctattg gcaccaaaat aatattgtca gaatcttgtc ggtgcctgtg caagaaacat
     4441 tcatgataat tcaaccaatt tgaataaaaa tgcagtacat taagagagaa gctaggtttt
     4501 ctagcttctc ttttattatt tatacaattg tagtgaattt actgaatcgt gttttgcaca
     4561 ggcacctgaa ttccaggtgg atatttctaa accatcttgc aggtcaaaca ggctttcttg
     4621 tcgaaacgta gacatatgaa attcattgaa ttggaggaaa aaacgatggc tgaagttttg
     4681 ttgttacacc atgtttgcgg tcgaacgaag gggattgaag cgatcgccga tcaactgagg
     4741 gatgcggggc atactgtgca cgtgccagat ttatttgacg gccgcgtgtt ttcttcgcta
     4801 gaagaaggct tggtatttgt taaggagatc ggatttgaag aagtgacggc gcgtggcgtt
     4861 cgggcggcca gcgagctttc acgcgatgtc gtctatgctg gtttttcact tggtgtcccg
     4921 gcagcacagc agctcgccca aacccgtgaa ggcgctagag gcgcactgtt ttttcatgcc
     4981 tgcctcccga cctcagcgtt cgaatccgag tggccagccg acttgcctgt gcaaattcac
     5041 gccatgaatg cagatccttt tttcgttgaa gacggcgaca tcgacgcggc ccgtgaactt
     5101 gtggcgtcgg ccaatcatgc tgagcttttt ctgtacgaag gcaaagaaca tctcttcacc
     5161 gacagcagtt taccatccta cgatgctggc gcgacgaaac ttgtgatcaa gcgggttctc
     5221 gactttctcg cataattggt aacgagaaaa ctttcagcag cttggatata actatactaa
     5281 gggcagtaac atatcccgcc aaaaaaacgc tctgattgag cgttttttgg cgaaatactt
     5341 tactatcttt attaaactaa tgcacccgtt agttcaatta gaaatcttct tatattaact
     5401 gccgcaatta ggaaaaatat cattcataag tagggcgaga cataagttca tttaaaaaat
     5461 tgaggttgta ataaacaact aaaattaaac aggtcaataa gattaattgg atacatacgg
     5521 taattaattg tatttttatt gtgaaactct ttttataaag tacataagtt agtggtatag
     5581 aaagtaagac actaaaagaa attgaaaata actgtaaatg gtctaggtaa atattaacgt
     5641 atagtaatgg agaaatatct tgtatccatt gagctaagaa cgggagtata atgattatta
     5701 ttaaaaacat aattagcgca gttaatataa agactgtaga agtatagaaa aatccatatt
     5761 tcttcatctt tggatcctcc tagtaataat tcatctcaag atataaatta gacttacaac
     5821 tatacttatt taattgttac ttgaattagt aaatggcgtt gctcctattg aagtaaagca
     5881 cccgttagtt caattataaa tcttcttata ttaactgccg caattaggaa aaatatcatt
     5941 cata
//
LOCUS       DASULI010000419         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_616559, whole
            genome shotgun sequence.
ACCESSION   DASULI010000419 DASULI010000000
VERSION     DASULI010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616559"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFX34_09065"
     CDS             complement(<1..523)
                     /locus_tag="VFX34_09065"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEX5565099.1"
                     /translation="MEDKKKLQEIEKLLKELKDSNSESAVTVEELIGKKRKSWRPRMP
                     FRFLSMWKRWTLLLAVLLLIAVISLPFVAFRFLTLGSTFTEQKGVFLEQIQELNEMAT
                     AEAYTKVLIERQDNQLFGQSIGLNVPGTKRQLLVVIPGSVKAGVDLSKLTEKDVVIDE
                     KKKTAKLTLPAAKF"
     gene            878..1711
                     /gene="thiD"
                     /locus_tag="VFX34_09070"
     CDS             878..1711
                     /gene="thiD"
                     /locus_tag="VFX34_09070"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX5565100.1"
                     /translation="MTMKKTLAIAGSDTSGGAGIQADLKTFQEHGTYGMTALTVVVTM
                     DPDNNWSHSVYSLPIDVLKAQLKTALSTGIDAIKTGMLSTEEVIQTAGEAIAESGLDH
                     VVIDPVMVCKGEDEVLNPGTVDAMVQYLLPKAEIVTPNLFEAGQLASMKTPSTIEQMK
                     SVAEKIHSLGARNVVIKGGKQLQHDKAADLFYDGQTFTLLEAEKTATHYNHGAGCTFA
                     AAITANLANGLDVKEAVIEAKKFVSAAIANGWKLNEYVGPVMHGAKNRVGAPEITATE
                     I"
     gene            1996..2346
                     /locus_tag="VFX34_09075"
     CDS             1996..2346
                     /locus_tag="VFX34_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008176033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HEX5565101.1"
                     /translation="MELVKPDHLQELLNSYANKDVYIHLETTNGAYAAHFKEGFFNAG
                     AFIRNVVVKYELGKVAGESPHRVGLKLPSGWIYAQGITHYTVDEHDRLLMAGLDPDGK
                     LAVALEISETPFTY"
     gene            2375..3046
                     /gene="bshB2"
                     /locus_tag="VFX34_09080"
     CDS             2375..3046
                     /gene="bshB2"
                     /locus_tag="VFX34_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HEX5565102.1"
                     /translation="MIKEERHVLIILPHPDDEAFGLSGTISTYIEMGVPVTYACLTLG
                     EMGRNLGNPPFANRETLPQIRKQELKNAAAAMGLTDLRMMGLRDKTIEFEDDEKMIKL
                     VADLIEDTNPSLIVTFYPGLAVHPDHDATGRAVVNAVGRMKESERPTLYTIAFAHNTA
                     EVLGAPDIIHNVEGASQKKLNTLNAHASQTVWMMRDMEKRLAEKDPEALKWLHEEKFF
                     TYKFE"
     gene            3219..>4529
                     /locus_tag="VFX34_09085"
     CDS             3219..>4529
                     /locus_tag="VFX34_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEX5565103.1"
                     /translation="MKKYHLSTWLYFLIPSILGIILFMIPLKFGEEWKVPIAKFADIL
                     SGWLEPAMPMTAMIIIVIAAIGSLLYAFIPRSSSKPSLLEGLFKVTPFWVVTRILGAI
                     FAIMVTFQIGPEAIWNENTGGLLLAPDGLVSFLFTIFLFAGLLLPLLLNFGLLEFFGT
                     MMVKVMRPLFKLPGRSSIDALASWIGDGTIGVLLTSKQYEDGHYTQREAAIIATTFSV
                     VSITFSLVIIDTVGMSNYFLPFYGTVLLTGVVLALIMPRIYPLRGKKTEYIDGRKFTG
                     EEEQLPEGYNVFSHGLENALDTAEKNRSIGRIFTDGMRNVLDMWIGVAPVVMAFGTVA
                     LILAEYTGVFTFLGKPFEPILSMLGIPEAAAAAQTMVVGFADMFLPVILADGAITSPI
                     TLFTIATISVVQLIYMSEVGGLILGTKIPINFLDLVIIFLLRTLI"
BASE COUNT         1164 a         1106 c         1077 g         1182 t
ORIGIN      
        1 ggaacttcgc ggcaggcaaa gtgagctttg ccgtcttctt cttttcatcg atgactacat
       61 ctttttcggt cagcttggac aaatcgacgc ccgccttcac cgatccggga atgacgacga
      121 gcagctgccg cttcgttccc ggaacattca atccgatgct ttgaccgaat aactggttgt
      181 cctggcgttc gatgaggact ttcgtatacg cctccgcggt cgccatttca ttcaactctt
      241 gtatttgttc aaggaatacg cctttctgtt ccgtgaaggt gcttcctaat gttaaaaatc
      301 gaaatgcgac gaatggaagg gaaatgacgg caattaagag aagcaccgct agtaatagag
      361 tccatcgttt ccacattgaa aggaatctga acggcatgcg tggacgccaa gattttcttt
      421 tcttgccgat caactcttcc actgtgacgg cactttctga attcgagtcc ttcaattctt
      481 tcagcagttt ttcaatttcc tgcaatttct ttttatcttc cataccctat ttcaccttcc
      541 ttcgacactc gtaatcgcgc atttttacat atagttatac gtttagacta tctaaaagtt
      601 tcgaagcgga caacagataa gcatgaggca tgctgccttt cattccaaaa cttgcaaata
      661 cgcataaatt cgtagactcg cgcataaaat tgtccattca ctcataaatc gccgcagacg
      721 cgcataaatc cccattttcg ctcataaatc ccctctatca cgcataaacg actaaattca
      781 cgcataaacc gccacgcgat tggaataatt gtatttttcc ttcaatttga ttactattaa
      841 atatgaatgc aatccaacaa gaaggagaaa tccaactatg acgatgaaaa agacattagc
      901 gatcgcaggt tcagatacgt caggcggcgc gggcatccaa gccgacttga aaacattcca
      961 ggagcacggg acatacggca tgaccgcttt gacggtcgtg gtgacgatgg atcccgataa
     1021 taattggtct cactccgtct attcacttcc gatcgatgtg ttgaaggcgc aactgaaaac
     1081 agctctttcg acaggcatcg acgcgattaa aacgggcatg ctcagcacgg aggaagtcat
     1141 ccagactgca ggtgaggcga ttgcggagtc cggtcttgac catgtcgtca tcgacccggt
     1201 catggtttgt aagggcgaag atgaagtcct caatccggga acggttgacg cgatggtgca
     1261 atacttgctt ccgaaggcgg aaattgtgac gccgaacttg tttgaggcgg gccaacttgc
     1321 tagcatgaaa accccttcta cgattgagca gatgaaatcg gttgccgaaa aaatccattc
     1381 gctcggcgct cgaaatgtcg tcatcaaagg cggtaagcag ctacagcacg acaaagcggc
     1441 ggacctgttc tacgacggtc aaacgttcac attgcttgaa gcggaaaaaa cagcaacgca
     1501 ttataatcac ggcgcaggct gcacattcgc cgctgccatt actgcgaacc tcgcaaacgg
     1561 ccttgacgtg aaagaagcgg tcatcgaagc gaagaaattc gtttccgcgg ccatcgcgaa
     1621 cggctggaag ttgaacgaat atgtaggacc cgtcatgcac ggcgcgaaaa accgtgtcgg
     1681 cgcaccggaa atcacagcga cagagattta atcttataaa tggccggaag agaatcgcct
     1741 cttccggctt ttttcgtgcg ggcaaaaagt tatcggtcat tttccaacgg atatcgatca
     1801 tttcttgaag tttatcgatc aattcagcga agttatcggt catttcccgg aacttaacgg
     1861 tcaatttcac atatttatcg tcatttcatg acctttgacc tacacacttt taaagtttca
     1921 agcctcgtca atcgcattca tttacacttt tctatatact tgttatatgt gattttgtaa
     1981 gaaggaggat ttttcatgga actcgtgaaa ccggaccatt tgcaggaatt gctgaattcc
     2041 tatgcgaata aagacgttta catccatctt gaaacgacga atggcgcata tgccgcccac
     2101 ttcaaggaag gatttttcaa tgccggggca ttcatccgga atgtcgtcgt caaatatgag
     2161 cttggcaagg tggcgggcga atcgccgcat cgtgtcggct tgaagcttcc gtcgggctgg
     2221 atctatgcgc aaggcatcac ccattacacg gtcgacgaac atgaccggct gctcatggca
     2281 ggtctcgatc cggacggaaa acttgccgtc gcgcttgaaa taagcgaaac accgtttaca
     2341 tattaagcag ttcgattgtg aaggaggctt ttcgatgatt aaagaagaac gccatgtgct
     2401 catcatatta ccgcacccgg acgacgaagc tttcggcctc tcgggtacga tttccacata
     2461 tatcgaaatg ggggtccctg tcacatacgc ctgtctgacg ctgggcgaaa tgggacggaa
     2521 tctaggcaat ccaccattcg cgaaccgtga aacgctgccg cagatccgca agcaggaatt
     2581 gaaaaacgcc gcagccgcca tgggattgac agacctccgg atgatgggct tgcgcgacaa
     2641 gacgattgaa ttcgaggatg acgagaagat gatcaagctc gtcgccgatt tgattgaaga
     2701 tacgaatcct tccctcatcg tcacgttcta cccgggactc gccgtccatc cggaccatga
     2761 cgcaacagga cgcgccgtcg tcaatgccgt cggtaggatg aaggaatccg agcgcccgac
     2821 gctgtatacg atcgcgttcg cacacaatac cgcagaagtg ctcggagcgc ccgacatcat
     2881 ccataacgtc gaaggggcgt cgcagaaaaa actcaacaca ttgaacgcac acgcttctca
     2941 aaccgtctgg atgatgcgcg atatggaaaa aaggttggcg gagaaagacc ctgaagcatt
     3001 gaaatggctg cacgaggaga agtttttcac atataagttt gaatagacac aagcagccgc
     3061 gccccgttgg caattgcaaa cggggtattt tcatagcccg aacggaagac tgaaaagatc
     3121 gatttctttc cattgtattt acaatcggat taaaacccga taatatgtat gattatacaa
     3181 gaatattgat actttattca taaaggagca agaacgagtt gaaaaaatat catttatcca
     3241 catggctcta ctttttaatt ccatccatac tcggcatcat ccttttcatg atccctctga
     3301 aattcggaga agaatggaag gttccgatcg caaaatttgc tgatatccta tccggctggc
     3361 tcgagcccgc catgccgatg acggcaatga tcatcattgt aatcgcggca attgggtcat
     3421 tgctgtatgc attcatcccg cgttcaagtt ctaaaccttc cctattggaa gggttgttca
     3481 aagtgacgcc gttctgggtc gtcacccgta tacttggtgc cattttcgcc atcatggtca
     3541 cgttccagat cggacctgag gcgatctgga atgaaaatac aggcggcttg cttcttgcgc
     3601 cggatggcct cgtttcattc ctgttcacga ttttcctttt cgcaggtctt ttattgccat
     3661 tattattgaa cttcggtctg ttggagtttt tcggtacgat gatggtgaaa gtgatgcgtc
     3721 cattattcaa actgcctgga cgttcctcca tcgatgcgct cgcttcatgg atcggcgacg
     3781 gtacaatcgg cgtcctgttg acgagcaagc aatatgagga cgggcattat acacaaaggg
     3841 aagcggcgat cattgcgacg acgttttcgg ttgtatcgat tacgttctcc cttgtcatca
     3901 tcgacacggt cggcatgtcg aactacttcc ttccgtttta cggaacggtg ttgttgacag
     3961 gtgtcgtatt ggcacttatc atgccgcgta tttatccgct cagaggcaag aaaaccgagt
     4021 atatcgatgg ccgtaagttc acgggcgaag aggagcagct gcctgagggc tataacgtgt
     4081 tcagccatgg cctggaaaat gcgttggata ctgctgaaaa gaaccgttcc atcggcagga
     4141 tcttcacgga cggcatgcgc aatgtgctgg atatgtggat cggcgtcgcg ccggtcgtca
     4201 tggctttcgg tacggtagca ctcattctgg ccgaatatac tggtgtcttc acgtttttag
     4261 ggaagccatt cgaaccaatt ttatcaatgt tgggcatccc ggaagctgcg gcagccgctc
     4321 agacgatggt cgtcggtttc gcggacatgt tcctgccagt cattttggct gacggcgcaa
     4381 tcacttcccc tattacattg tttacgattg cgacaatctc agtcgttcag ctcatttaca
     4441 tgtccgaggt cggcggtttg attctcggaa cgaaaatacc tattaacttc ttggatttag
     4501 ttatcatttt cttattgcgt acactcatt
//
LOCUS       DASULI010000420         4225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1219624,
            whole genome shotgun sequence.
ACCESSION   DASULI010000420 DASULI010000000
VERSION     DASULI010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219624"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VFX34_09090"
     CDS             <1..957
                     /locus_tag="VFX34_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012959581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5565104.1"
                     /translation="GSGPATVAAIGGIMIPAMVRNGYDKRFATAVVAAAGSIGVIIPP
                     SIPMVIYGVVGNASIGDLFIAGIIPGILVGLSMIIYAYFYSKKKGFKGMDEPTSFKKI
                     GVAIWEAKWALLIPVIILGGIYGGYFTPTEAAVIAVVYGLFASVVLYRELRIKDLPKV
                     MVDSALTTATVLIIVGTATAFGRYLTIEQVPMKVANALMNVSSEPIIIILLITALLLV
                     VGMFMDTLAAIIILTPILLPIAIQIGYDPIHFGILMVVNLAIGFITPPLGVNLFVASG
                     ISGLSLESLSRAVVPFVFAMIFTLLLITFIPQLSLFLVGFAK"
     gene            978..1943
                     /locus_tag="VFX34_09095"
     CDS             978..1943
                     /locus_tag="VFX34_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX5565105.1"
                     /translation="MNEIDVFTLGETMVLFQPEQSLPMEYVHQFPKSIGGAESNVAIG
                     LTRLGHSVGWFSKLGNDPFGRYILKSVRGEGVDVSSCLMTDDAQTGLIFKERLTPEDV
                     NVYYYRKNSAASLMEEADLNEPYIAQAKILHITGITPALSESCYRTVMKAIEIAKKNG
                     MKIVFDPNLRLKLWSAERAKSVFNEIASYADVILPGLDEGQFMTGMVEVEEVADALMG
                     DQDKTIIIKLGSKGAYLRTKDEKAYIDGFPVQQVVDPVGAGDGFAAGIISGILRQEPL
                     PLVVKRANAIGAMVVGVHGDVEGLPTLSAVERFMEPAEAVRDVKR"
     gene            1962..2606
                     /gene="eda"
                     /locus_tag="VFX34_09100"
     CDS             1962..2606
                     /gene="eda"
                     /locus_tag="VFX34_09100"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX5565106.1"
                     /translation="MKKWEDLLRLKESGLVAVIRRPDPSQIHSIAEALILGGVGALEI
                     TVDTPDAFTMIKSLKGTFKDRVLVGAGTVLDAESAKAAIDAGSDFIFSPSFDVKTIQI
                     TNRYGKISIPGVMTPTEIVQAYSAGADILKIFPGNALGAGYLKDLQGPLGHIPMMPTG
                     GVSLDNVEAFIKNGAVAVGVGGTLVDGKAIAEERYDELTKIAKQFIMKIQQARN"
     gene            2646..3794
                     /gene="dgoD"
                     /locus_tag="VFX34_09105"
     CDS             2646..3794
                     /gene="dgoD"
                     /locus_tag="VFX34_09105"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEX5565107.1"
                     /translation="MKITKMETFIVPPRWLFLKIETDEGIVGWGEPVVEGRAATVEAA
                     VKELSGYIIGKDPLRIEDHWQTMYRAGFYRGGPILMSAIAGIDQALWDIKGKFYDAPV
                     YDLMGGACRDSVRVYSWIGGDRPSDVGKAAKEAVAAGFTAVKMNGTEELQYIDSYQKI
                     DEAVERIAAVREAVGNHIGIGIDFHGRVHKPMAKLLAKELEQFRPMFIEEPVLPENNE
                     ALREVARATNIPIATGERMYSKWDFKKLLEDGYVDIIQPDLSHAGGITECKKIFSMAE
                     AYDVAVAPHCPLGPIALASCLQVDATSHNVFIQEQSLGIHYNQGSDLLDYLNDPKVFD
                     YKDGYVDMLHGPGLGISINEEFVREQAKIGHNWKNPVWRHKDGTIAEW"
     gene            3853..>4225
                     /locus_tag="VFX34_09110"
     CDS             3853..>4225
                     /locus_tag="VFX34_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006829542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX5565108.1"
                     /translation="MSRFKVVVTDYEYETFAPEQEVLRKIGVELTLAQCHTEEEVIAA
                     CKDADALISQYAPISRNVIENLEKCKVIARYGVGFNTIDVEAATEKGIIVGNVTDYCV
                     DEVSDHTIALLLASARKITTLN"
BASE COUNT         1109 a          886 c         1156 g         1074 t
ORIGIN      
        1 gggtccggcc ctgctactgt tgcggcaatc ggcggaatca tgatcccggc aatggtccgc
       61 aacggctatg acaagagatt tgccacagca gtcgttgctg cggcgggttc gatcggagtc
      121 atcatccctc cgagtattcc catggtcatt tacggtgtcg tcggaaacgc atccattggt
      181 gatctattca tcgccggaat catacctggg atcctggtcg gactttcgat gatcatttac
      241 gcgtattttt attcgaagaa aaaagggttc aaagggatgg atgagccgac gtccttcaag
      301 aaaataggcg ttgccatatg ggaagccaaa tgggctttgc tgattccggt cattatttta
      361 ggcgggattt acggaggata tttcacgccg acagaagcgg cggtcatagc ggttgtttat
      421 ggactattcg cttccgttgt cttatatcgt gagcttcgga ttaaagattt gccgaaagtc
      481 atggtcgatt cggctttgac gacagcaact gtattgatca ttgtcggaac agcaactgcg
      541 ttcggaagat acttgacaat cgaacaagtt ccgatgaaag tggcaaacgc tttgatgaat
      601 gtttccagtg agccgatcat catcattttg ctcatcacgg cgcttttatt ggttgtcggg
      661 atgttcatgg acacactcgc tgccattatc attctgactc caatcctatt gccgatcgct
      721 attcaaatcg gctatgatcc aatccatttc gggattctga tggtcgtcaa cttggcgatc
      781 gggttcatca caccgccgct tggagtgaat ctgtttgtcg cttcaggaat ttccggactt
      841 tcgcttgagt cattatcccg tgcggtagtc ccatttgttt ttgcaatgat cttcacctta
      901 ttgctgatta cattcattcc gcagctttcg ctattcttgg tagggtttgc gaaatgatga
      961 aaaggcaggt tctataaatg aatgaaatcg atgtgtttac acttggagaa acgatggtcc
     1021 tttttcaacc tgaacaatct ctccctatgg aatatgtgca tcaatttccg aaaagcatcg
     1081 gcggcgccga atcgaatgtt gcgatcggct tgacgagatt gggccattct gtcgggtggt
     1141 tcagtaaatt ggggaatgat cccttcggaa gatacatcct caaaagtgtt cgtggagaag
     1201 gcgtggacgt ctcttcctgt ctgatgacgg atgatgcaca aacaggtttg atttttaaag
     1261 agcgattgac gccggaagat gtaaacgttt actattaccg caaaaattct gcggcaagtt
     1321 taatggaaga agctgatttg aatgaaccct atattgcaca agccaaaatt ctgcatatca
     1381 cggggataac gcccgccctt agcgaatcct gttatcggac tgtgatgaaa gcaattgaaa
     1441 tcgcgaaaaa gaacggcatg aagattgtct ttgatccgaa tttacgcctc aagctgtggt
     1501 ctgcggagcg cgccaaaagc gtgttcaatg aaatcgcttc ttacgcggat gttattttgc
     1561 ctggccttga cgaagggcaa ttcatgacag gcatggtgga agtggaagag gtggctgatg
     1621 cgttaatggg tgatcaggat aagacgatta tcataaagct cggtagtaaa ggggcctatt
     1681 tacggacaaa ggacgaaaaa gcatatatag acggattccc tgttcagcag gtcgttgatc
     1741 cagtaggggc aggcgatggt tttgcagctg ggatcatcag tgggatttta cgtcaggaac
     1801 cgctgcccct tgtcgtcaag cgggcgaatg ccatcggagc aatggtcgtc ggtgttcatg
     1861 gcgatgtgga aggcttgccg acactttccg ctgtcgaacg attcatggaa ccagcagaag
     1921 ctgtacggga tgtgaaaaga tgagaaaggg agcgtgcacg catgaaaaaa tgggaggatc
     1981 ttttacggct taaagaatcg ggattggtcg ctgtcattcg tagacccgac ccatcacaga
     2041 tccattcaat tgcggaagcg ctcattctcg gtggagtcgg tgctttggaa ataacagtcg
     2101 ataccccgga cgcttttacg atgatcaaaa gcttgaaagg tacattcaag gatcgagtcc
     2161 tcgttggagc gggcacggtg ctggatgccg aatcggcaaa agcggcaatt gacgcaggct
     2221 cggattttat cttctctccc agtttcgatg tgaaaacgat ccagattacg aaccgctacg
     2281 gaaagatttc catcccgggc gtcatgaccc cgacagaaat tgtccaagcc tattcggcgg
     2341 gggcggatat tcttaaaatt ttcccgggca atgcactggg tgccggatac ttgaaagatc
     2401 tacaaggtcc gctcggtcat atcccgatga tgccgacagg cggagtgagt ctggataatg
     2461 tggaggcgtt cattaaaaat ggcgcggttg cagttggagt cggcggaacg ttggttgacg
     2521 gaaaagcgat cgccgaagag cgttatgacg agcttacgaa gattgccaag cagttcatca
     2581 tgaaaatcca acaggcaagg aactgaaatt cccgcttgct gttgcaataa agggaggtct
     2641 tacacatgaa aatcacgaaa atggagacat tcatcgtacc gccaagatgg ctgttcctga
     2701 agattgaaac ggatgaagga attgtcggat ggggagagcc ggtcgtcgag ggacgtgcgg
     2761 ctactgtaga agcggccgtc aaggaattaa gcgggtatat catcggcaaa gaccctctcc
     2821 gtattgagga tcattggcaa acgatgtacc gggccggttt ttaccgggga ggtccaatac
     2881 tcatgagcgc aatcgcaggg attgaccagg ctttatggga tattaaaggt aaattttatg
     2941 atgcgcctgt ttatgatttg atgggtggtg cctgccggga ttcggtccgc gtttattcct
     3001 ggatcggggg cgaccgtccg agtgatgtag gcaaagcggc gaaagaggcg gttgctgccg
     3061 ggtttacggc cgtaaagatg aatgggacgg aagaacttca atatattgac tcttatcaaa
     3121 agatcgatga agcagtcgaa cgaatcgcag ccgtccgtga agctgtcggg aatcatatcg
     3181 gaatcgggat cgacttccac ggacgtgtac ataaaccgat ggctaaacta ttggccaagg
     3241 aacttgagca gttcaggccg atgttcatcg aagaacctgt attgccggag aacaacgaag
     3301 cccttcgtga agtcgcgcgt gccacgaata ttccaatcgc gacaggggag cgcatgtatt
     3361 ccaaatggga ttttaaaaag ctgctcgaag acggctatgt cgatatcatc caacccgacc
     3421 tgtcccatgc aggcggaatc acggagtgca aaaaaatctt ctcgatggct gaagcgtatg
     3481 atgtagcggt tgccccacat tgtccactcg gtccgattgc attggcatct tgccttcaag
     3541 tggatgcgac atcccataat gtcttcatcc aggagcagag tttaggcatt cactacaacc
     3601 aaggcagtga tttgctggat tacttgaatg atccgaaggt atttgactat aaagatggct
     3661 atgttgacat gctgcatgga cctgggctgg gtatttcaat caatgaagag tttgtaaggg
     3721 aacaagcaaa gatagggcat aattggaaga atcctgtgtg gcgccataaa gatggaacca
     3781 ttgccgagtg gtaagtgatg ttcatattca ttcaaatagt agtacgagtc ggggcgagaa
     3841 ggagtggctg atttgagccg ttttaaagtc gtagtgacag attatgaata cgaaacattt
     3901 gcaccagaac aagaggtgct gcgtaagatc ggtgtagaac tgaccttggc gcaatgccat
     3961 acggaagagg aagtcattgc ggcgtgcaag gatgctgacg cattgatcag ccaatatgcc
     4021 ccgatcagcc ggaatgtaat tgaaaatctt gagaaatgta aggtgattgc ccgctatgga
     4081 gtcggcttca atacaatcga tgtggaggcg gcaacagaaa aaggcattat cgtcgggaac
     4141 gtgactgact attgtgtgga tgaagtatcg gatcatacaa tcgctttgct tcttgcaagt
     4201 gcccgcaaaa taacaactct taata
//
LOCUS       DASULI010000421         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1881903,
            whole genome shotgun sequence.
ACCESSION   DASULI010000421 DASULI010000000
VERSION     DASULI010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881903"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /gene="deoA"
                     /locus_tag="VFX34_09115"
     CDS             <1..465
                     /gene="deoA"
                     /locus_tag="VFX34_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEX5565109.1"
                     /translation="VAGEKAETVEEARSMLEAVIKDGSALNLFGKLIEAQGGDARIVH
                     DPSLLPSAKFQIEVPALTSGTISKMVADEIGVAAMLLGAGRATKDDQIDLAVGIVLKK
                     KIGDNVTAGEPLAIIHANQEDVAKSIELIQKHIHISDQAQEKPALIHEMITG"
     gene            815..1174
                     /locus_tag="VFX34_09120"
     CDS             815..1174
                     /locus_tag="VFX34_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEX5565110.1"
                     /translation="MSIEMKNDYGTIDITNDVIAQVVGEAAIECYGIVGMASRHQIRD
                     GLTDILGKENYARGVIVRNIGDETHIDVYVIIGYGTKISEVAYQVQSKVMYTLKKILS
                     LTVDSVNIFVQGVRVTN"
     gene            1196..>2461
                     /locus_tag="VFX34_09125"
     CDS             1196..>2461
                     /locus_tag="VFX34_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX5565111.1"
                     /translation="MNFIDGVKFADMVRMGAHHLHLNADYVDSLNVFPVPDGDTGTNM
                     NLSMTSGAKETEANVSAHLGKTAQALSKGLLMGARGNSGVILSQLFRGFGKAVEEEEK
                     LDSKKFANALEYGVQTAYKAVMKPVEGTILTVAKDAANKGVEVAKSTDDIIKVMEAIV
                     EESKASLKRTPDLLPVLKEVGVVDSGGQGLVYVYEGFLACLKGEELPERTNMDLMEDL
                     VNAEHHRGVQGFMNTEDIVYGYCTEFMVKFEDEKVKKHPFEEGDFRQDLSKFGDSLLV
                     ISDEEIAKVHIHTEEPGDALNYAQQYGSLINIKIENMRQQHIDIVGESHSTKKAEAVK
                     HPYAIVTVAMGSGIADLLKSIGASAVIEGGQTMNPSTEDIVKAIKDVSAERVIILPNN
                     KNIIMAAKQAAEVIGIEAAVVETKSVPEGL"
BASE COUNT          759 a          476 c          655 g          571 t
ORIGIN      
        1 gttgccggtg aaaaagcgga aacggttgaa gaggcgcgct caatgctgga agcagtcatc
       61 aaagacggtt cggcattgaa tttattcggg aaattgatcg aagcgcaggg cggagatgca
      121 cggatcgtcc acgacccatc attgctccct tcggcgaaat tccaaatcga agtccctgcc
      181 ttaacatcag gcacgatttc caaaatggta gcggatgaaa tcggagtcgc agcaatgctt
      241 ttaggagctg gaagagcgac aaaagatgac caaatcgact tagcagtcgg catcgtcttg
      301 aagaaaaaaa tcggcgacaa tgtaaccgcc ggcgaaccac tagccatcat ccacgccaac
      361 caagaagatg tcgcaaaatc aatagaatta atccaaaagc acatccacat ttcagaccaa
      421 gcacaagaaa aaccagcctt gatccatgaa atgattacag gataaaaata tataatatag
      481 ggctgcccgg aaagtcaaaa caccgactct ccgtggcagc cttttttgtg ttaatgcggc
      541 tatatgatca cttatccgga tttatgagcg ctaaggacgt tttatgatca ataacaggga
      601 attcctaaat aataggaaac acagttgatt gaagtgtcga gcggcgactc cagcgaaagc
      661 cgtaacgaag aacggctttt gcgagtaaaa gcgtagcgtt acgagcaggg aggagcaagc
      721 gccctgcgct tttgtcgggt acttgttctt ttataatgtc agcgtgtata gtacaatggg
      781 gttagttatt atggatgcag gaggggaagc tttcatgtca atcgagatga aaaacgatta
      841 cggtacgata gatattacga acgacgtcat agcccaagtg gtgggagagg cggcaatcga
      901 atgctacgga attgtcggca tggcttcacg tcatcaaatc cgggatgggc ttacggacat
      961 ccttgggaaa gagaactatg caagaggggt cattgttagg aacatcggag acgaaacgca
     1021 tatcgacgtg tatgtcatta tcgggtatgg aacaaaaatc tccgaagtcg cataccaagt
     1081 gcaatccaaa gtgatgtaca cattgaaaaa gatattatcg ttaacagtgg attcagtgaa
     1141 tatatttgtt caaggagttc gggtgacgaa ctgatagaag gaggaaaaca tttcaatgaa
     1201 ttttattgat ggtgtgaaat ttgccgacat ggtgaggatg ggtgcacatc accttcatct
     1261 gaatgcggac tatgtcgatt cactaaacgt gtttcccgta cctgacgggg acacaggaac
     1321 aaatatgaat ttatccatga cttctggtgc caaggagacc gaagcgaatg tgtctgcgca
     1381 tttgggcaag accgctcaag ctttgtctaa agggctattg atgggagctc gcgggaactc
     1441 gggtgtcatc ttatcgcaat tgttccgcgg tttcggaaaa gctgtagaag aggaagaaaa
     1501 gcttgatagc aagaagttcg caaatgcatt ggaatacgga gtgcagacag cgtacaaagc
     1561 ggtcatgaag ccagtagagg gaacaatcct cactgttgct aaagatgcag cgaataaagg
     1621 tgtcgaagtg gcgaaatcta cagatgacat cattaaagtg atggaagcaa tcgttgaaga
     1681 atccaaagct tctttgaaac ggactcctga tcttctgcct gtgttgaaag aagtaggagt
     1741 cgtcgatagt ggcggacagg gccttgtcta tgtatatgaa ggctttttgg catgtctgaa
     1801 aggtgaagag cttcctgaac ggacgaatat ggacttgatg gaagatctag tgaacgcgga
     1861 gcatcatcgc ggcgtccagg ggttcatgaa cactgaggat atcgtatacg gatattgtac
     1921 ggaattcatg gtgaaattcg aggatgaaaa agtgaagaag catccattcg aggaaggcga
     1981 tttccgtcag gatctcagca agttcgggga ttccttgctt gtcatttcag acgaggaaat
     2041 tgcgaaagta catatccata ctgaagagcc gggagatgca ttaaattatg cacagcaata
     2101 cggttccctt atcaacatta aaatcgaaaa catgcgacag cagcatatcg atattgttgg
     2161 agagagccat tcgaccaaaa aggcggaggc agtcaaacat ccttacgcaa tcgtgacagt
     2221 ggcgatgggt tcaggaattg ctgatttgtt gaagagcatc ggtgcttcag ccgttatcga
     2281 aggcggtcag acgatgaatc catcaactga ggatatcgtc aaggcaatca aagatgtgag
     2341 cgctgagcga gtcatcattt tgccgaacaa taaaaatatc atcatggcgg caaagcaggc
     2401 ggcagaagtg attggcatag aagcggcggt cgttgaaacg aaatccgttc ctgaaggatt
     2461 g
//
LOCUS       DASULI010000422         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1686403,
            whole genome shotgun sequence.
ACCESSION   DASULI010000422 DASULI010000000
VERSION     DASULI010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686403"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..378)
                     /locus_tag="VFX34_09130"
     CDS             complement(250..378)
                     /locus_tag="VFX34_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HEX5565112.1"
                     /translation="MEQWLELIQQIGFPIFVSFYLLHRLETKLEAIHNALVSLNMK"
     gene            complement(724..942)
                     /locus_tag="VFX34_09135"
     CDS             complement(724..942)
                     /locus_tag="VFX34_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HEX5565113.1"
                     /translation="MAKTLQLIFITTEGKQTSLTVDEPKDGLTPSEVEAAMQQIISSS
                     VFQVDGYPLETAKGARIVERTVTELVEA"
     gene            complement(963..1181)
                     /locus_tag="VFX34_09140"
     CDS             complement(963..1181)
                     /locus_tag="VFX34_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HEX5565114.1"
                     /translation="MATIEFRHAVGRVYFDGGMTEGGKMIRIAKTYRNLAEGATAENL
                     HEALSQLAQLSNYPFIGAEKVETADIMN"
     gene            1316..1723
                     /locus_tag="VFX34_09145"
     CDS             1316..1723
                     /locus_tag="VFX34_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2621 domain-containing protein"
                     /protein_id="HEX5565115.1"
                     /translation="MWFIIFWCVVLIGLFGIGGFFMFRKFLKVFPKADGKSTLDWEEH
                     YVNESLHLWNEEARDLLNELVSPVPELFRDVAKQKIGAKIGQLALEDRAKTVSFDHII
                     RGYILATPKRDHKFLRRKLDSMEIDISPYEHLF"
     gene            complement(1749..2273)
                     /locus_tag="VFX34_09150"
     CDS             complement(1749..2273)
                     /locus_tag="VFX34_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdC"
                     /protein_id="HEX5565116.1"
                     /translation="MLKLQELIDFIFDEIPSVYLIVGSTVLFLFMGTLAFIVRTKAAK
                     RPISPKRIILPPLFMSTGALMFLFEEFRVPWPQVIESICVGMLFSIVLIKTTNFEAKD
                     EKLYVKKSRAFLVILFGLLIVRFVAKLILSSSIDVGELGGMFWILAFGMIVPWRIGML
                     VKYSKLKKHVEKAS"
     gene            complement(2266..2622)
                     /locus_tag="VFX34_09155"
     CDS             complement(2266..2622)
                     /locus_tag="VFX34_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX5565117.1"
                     /translation="MIFEVEELPMKEIGSFEQWLDIKAGEPQLLLFVKTDHCSVCEGL
                     YPQVAELEEEYPFPFYKVNAAQVPEMAGQLALFTAPVVLLFHQGKETARFARFVQMEQ
                     LKHRMDELVEWGEEHA"
     gene            complement(2666..3376)
                     /locus_tag="VFX34_09160"
     CDS             complement(2666..3376)
                     /locus_tag="VFX34_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX5565118.1"
                     /translation="MATDLNIFLAFGAGFLSFISPCVLPLYPAFISYITGMSIEELKS
                     DSKRMNRNGMLHTIFFLLGFSIVFVFLGFSTSFVGNFFIQYKDLLRQIGAIFIVLFGL
                     MVIGLFTPKFLMQEHRLQFKNRPAGYLGTALIGLAFSAGWQPCMGPIIGAIIGLAAAN
                     PGSSMVYMMMYVLGFAIPFFVLSFFITRLGWIRKNSNLIMKIGGYIMIAFGLLLFFDG
                     IVFLTSLLSPIFGDFQGF"
     gene            complement(3811..5025)
                     /locus_tag="VFX34_09165"
     CDS             complement(3811..5025)
                     /locus_tag="VFX34_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEX5565119.1"
                     /translation="MHMNSTMNIPGLKDVNIEKIEEVGERTALHVSLPRQPHKCPKCG
                     AMTAKIHDYRIQKIKHLKWFERLTVLFYRRRRYACSCGKRFSETSPFVDKYQRYTKEW
                     NQVVRVRSVKAKTFKEAGEVLGTSSSTVIRRFKEVAKEQMIDGVRLPKAIAIDEYKGD
                     TDAGTYQLIIANAETHEPLDILPNRRKETIKDYLHKYGADVELVVMDMNPSFKAAVKK
                     ALGRPVIVADRFHYCRYIYWALDEVRRKVQKEWHAYDRKKCKRMRHVLYKRNEKLTEK
                     NRWYLDRYLGMSDELRRAYELKEAYCEWFDWAKNAKDMAEVKSRLEAFYRKVEKADIP
                     AFSRAIQTFKNWQIEILNSFGFKYSNGFLEGINNKTKVMKRNAYGFRRFDHFKAKILL
                     NIRYKEIGVHLG"
BASE COUNT         1406 a         1253 c         1069 g         1477 t
ORIGIN      
        1 gatacttgtt catgatgctg attcgtgaac tcaaacggat caatcttata cttatgatcc
       61 gctttaaatc cgcccattga acatgcgctc aataatagca taagcatcat gatggatgat
      121 agaaataatt tgttacgcat atgcagaccc ctccactttc agtacttagt ttatcatagt
      181 ggataggcgc taatcaaaca accgcgcagt ccaatcgttc ttgtccaatc tttgacgttt
      241 cagtgaagtt catttcatat tcaatgaaac aagcgcattg tgaatcgctt ccagtttcgt
      301 ttcaagacgg tgcaaaaggt aaaatgatac aaagatcgga aatccaatct gctgtatgag
      361 ctctagccat tgttccattt tgaaacctcc tttctataaa gcgcagggcg cctgcctagt
      421 gaatgttcgc agactaaaaa caccgcgtcc tgatagtgcc ttaatttctg caaaatacac
      481 agaaatacgg caaatcaaac ccttcgcagt tcgattggca acgactgcat gaccggcatc
      541 ctgcaggcct caggcataag gtgaagatgc gaaggctgct cgtaacgctg cgcttttact
      601 cgcaaaagcc gttcttcgtt aaggctctat ctttgactta tgactccgag cttctggcgc
      661 ccgtagctgg attgattgaa atttgaaaag gcccccagta cgggactggg agccgtttac
      721 acattatgcc tctactagtt cggtgaccgt ccgctcgaca atgcgagcac cttttgccgt
      781 ttcaagcgga tagccgtcca cttggaatac actggaactg atgatttgct gcatggcggc
      841 ctccacctcc gatggtgtca acccatcttt cggttcatca acggtaagcg acgtctgctt
      901 cccttctgtt gtgataaaga tcaattgtaa tgtttttgcc attccctatt cccccttttt
      961 cattaattca taatgtctgc tgtttccact ttttccgctc cgataaatgg ataattggac
     1021 agctgtgcca attgtgaaag agcttcatgc agattctccg ctgttgctcc ttctgccagg
     1081 ttacgatagg tttttgctat gcggatcatt ttcccgccct ctgtcattcc accgtcgaag
     1141 tacaccctcc cgactgcatg tctgaattcg attgtcgcca tttgctttcc cctcctttca
     1201 cacgttatat agacttgaaa aggaagaaag gatactttgt ccgtgaattt ttttaaaaat
     1261 gctcttataa tggggagtag aatggaagag gtgaagtcag ttgcaagact tttttatgtg
     1321 gttcatcatt ttttggtgtg tcgtcctgat cgggcttttc ggaatcggcg gctttttcat
     1381 gttccgcaaa ttcctgaaag tgtttccgaa ggcagacggc aaatcgacgc ttgactggga
     1441 agaacattac gtgaatgaat cccttcatct ctggaatgag gaagcgagag atttattgaa
     1501 tgaactcgtc agccccgttc cggaattatt ccgtgacgtc gccaaacaaa aaatcggggc
     1561 taaaatcggc caactcgcat tggaagaccg tgcgaagaca gtaagcttcg atcatattat
     1621 ccgcggttac attctagcca caccaaaacg cgatcataaa tttcttcgaa gaaaattgga
     1681 ttcgatggaa atcgatatat cgccttacga gcatttattt taaatgaatt ggaaaagcct
     1741 cctatgaatc aggaggcttt ttctacgtgt tttttcaatt tggaatattt cacaagcatt
     1801 ccgatgcgcc aagggacaat catgccgaat gcgagtatcc agaacatgcc gccaagctcg
     1861 ccgacgtcaa tcgagctgct taaaatcaat ttcgcgacaa agcgtacaat gagcaaaccg
     1921 aataggatga ctagaaaggc tctcgacttt ttgacgtata gcttttcatc tttcgcttca
     1981 aaattcgtcg ttttgatcaa tacaattgaa aataacatgc cgacacagat tgactcgatg
     2041 acttgtggcc acggcaccct gaactcctca aacaggaaca ttagcgcgcc tgtcgacatg
     2101 aacagtggtg gcaagatgat acgtttcggt gaaatcggtc ttttcgcagc cttcgtccga
     2161 acgatgaacg ccaacgtccc catgaaaaga aacaatacgg ttgagccgac gatcaggtag
     2221 acagagggga tttcatcgaa aatgaaatca atgagctcct gaagcttaag catgttcctc
     2281 cccccactcc accagttcat ccatccggtg tttcaattgc tccatttgga caaaccttgc
     2341 aaatctggcc gtttcctttc cttgatgaaa cagaagtacg accggcgcgg tgaataacgc
     2401 aagctgtccc gccatttcgg gaacttgtgc cgcattcact ttataaaaag ggaatggata
     2461 ttcctcctca agttcggcca cttgtggata tagcccctca cagacggagc aatgatccgt
     2521 tttgacaaac agtaatagct gtggctcacc cgccttgatg tccagccatt gttcgaaaga
     2581 tccgatctct ttcatcggca gctcctccac ttcaaaaatc aattatgcct ccaagcaaag
     2641 tgctcggagg cgttcgaatt attcgttaaa acccttggaa atccccgaat ataggactta
     2701 gtaagcttgt taggaaaaca atgccgtcaa agaacagcaa cagtccaaag gcaatcataa
     2761 tataaccacc aattttcatg atgagattgc tgtttttgcg aatccatcca agccttgtaa
     2821 tgaaaaatga caggacgaaa aatggaattg caaaaccgag cacatacatc atcatgtaca
     2881 ccatgcttga gcccggattc gctgcagcga gcccgatgat tgcaccgatg atcggcccca
     2941 tgcaaggttg ccaacctgca gaaaaagcga gcccgatcaa tgccgtccct aaataaccgg
     3001 caggacgatt tttaaattgc agccgatgct cttgcattaa gaacttcgga gtaaacaatc
     3061 cgatgaccat cagtccgaac agtacaatga aaatggcgcc tatttgtctg agcaggtcct
     3121 tgtactgtat aaagaaattg ccgacaaagg atgtgctgaa tcctaaaaag acgaatacga
     3181 ttgaaaatcc gagtaaaaag aaaattgtat gaagcatgcc attccggttc atccgtttcg
     3241 aatcggattt aagctcttcg atcgacatgc cggtaatgta agatatgaat gccggataca
     3301 atggcaggac gcatggcgaa atgaagctga gaaaacctgc gccaaatgct agaaaaatat
     3361 ttaaatcagt tgccatcatt ctctcccctt catttttcta ctcccatcgt aacaaatccc
     3421 tagctagaat tcgcttattt taactgtgaa caattaatga acaaagaaaa gcgaagaaga
     3481 gcggtgagct gcgacaggtg gtggagagct tgaacaaaag gcgctttttg ccttttgctt
     3541 caagattgaa gcgacccgag cagctgctcc ctcgtagcta gacaaagaaa ataaaggttc
     3601 taaatcaaat gttggggtga tgtgatttgc acgtcaccct ttcatttaga ttttgctatg
     3661 aataatgttg atttgcgcta caggcggacg ctttccgccg gcatggcttc agccgcttcc
     3721 ctcgctttgc tcagtccagg gtcttcagct catgctattc cggcaggagt cgccgccttt
     3781 cgctccaatc aacgaatccc ttttaaaccg ctatcctaaa tgaaccccaa tttccttata
     3841 tcgaatgttt aataatatct ttgctttaaa gtgatcaaaa cgcctaaaac cataggcatt
     3901 gcgcttcatc acctttgttt tgttattaat cccttccaga aaaccgtttg aatacttgaa
     3961 gccaaaacta ttcaagatct caatttgcca gtttttgaac gtttgaatag ctcttgaaaa
     4021 cgcgggaata tcagctttct ctaccttacg gtaaaaggct tctagtcgac ttttcacttc
     4081 tgccatatcc tttgcgttct ttgcccagtc gaaccactca cagtaggcct ctttcagttc
     4141 gtatgccctt ctcaattcat cagacatgcc tagatagcga tctaaatacc aacggttttt
     4201 ttccgtcaat ttctcgttgc gcttatataa tacatgccgc atccttttac atttcttgcg
     4261 gtcatatgca tgccattctt tctgcacttt ccgccgaacc tcatcaagcg cccagtagat
     4321 ataccgacaa taatggaatc tgtctgcgac aatgacgggg cgtcccaaag cctttttcac
     4381 agctgccttg aagctcggat tcatatccat gaccaccaat tcaacgtcag ctccgtattt
     4441 gtggagataa tccttaatcg tttctttcct gcggttgggt aagatgtcca aaggttcgtg
     4501 tgtttctgca ttcgcaatga tcagttgata cgttccggca tctgtatcgc ccttatattc
     4561 atcaatcgca atggcttttg gtaatcggac gccatcgatc atctgctctt tcgccacttc
     4621 cttgaaccgg cggatcactg tcgagctgga ggtgccgagt acttctcctg cttctttaaa
     4681 ggttttcgct ttgaccgatc ggacacggac aacctgattc cactctttcg tgtagcgttg
     4741 atacttatcc acgaaggggg atgtctctga gaagcgcttt ccgcatgaac aggcataccg
     4801 gcgacgtctg tagaaaagaa ccgtgagacg ctcaaaccac ttgaggtgct tgatcttctg
     4861 tatacggtaa tcgtggatct tagccgtcat cgccccacac ttagggcatt tgtgaggctg
     4921 cctaggaagc gaaacatgaa gtgcggtccg ctccccgacc tcctcgatct tttcaatgtt
     4981 tacatctttt aatcctggaa tattcatggt agaattcatg tgcacgtatc acctttcgtt
     5041 tacttgtttc tcgacaatcc aagtataaaa gaaatggtga ctacgtgctt ttttttgttg
     5101 aatttgtatg aacaccccaa caaatattat agaaccactt tattacaaaa aagaactgtc
     5161 acagaaagtc agtttcgact ttctggacag ttcttttaaa cgatt
//
LOCUS       DASULI010000423         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_744668, whole
            genome shotgun sequence.
ACCESSION   DASULI010000423 DASULI010000000
VERSION     DASULI010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744668"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..294
                     /locus_tag="VFX34_09170"
     CDS             118..294
                     /locus_tag="VFX34_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565120.1"
                     /translation="MQYSKTKMEQLVKNNAELTQRLKQIMKEHELEKSFALKALYHAE
                     VKESGRFMKDYQEL"
     gene            503..688
                     /locus_tag="VFX34_09175"
     CDS             503..688
                     /locus_tag="VFX34_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565121.1"
                     /translation="MQNRIEELEEARLKGLNQGIRQEKVSTVLTMHGLAFSTEKIALV
                     TRLTTQEIEAIILENWH"
     gene            864..>2084
                     /locus_tag="VFX34_09180"
     CDS             864..>2084
                     /locus_tag="VFX34_09180"
                     /inference="COORDINATES: protein motif:HMM:NF020044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEX5565122.1"
                     /translation="MSIQLTQNMIMDLCGTVSFKRGEMFNRAGKVTIEQYSETHCKAI
                     VAGKENFHVTIDLTDELPRTECSCPVLPFYKYKCQHVAAVMIAMIERKREEEPDLTEG
                     LLSLFNERPRARGHQLHFENREVLELQFTLKPVTVGKQLMLGIELAIEGIHVEDIRQF
                     LQDVNEGRASSLASAFAFDSEIHCFEREDEAVLQQLIDINHEEGAFGHRSVGLGTLRI
                     PSSSWEKLLQLLNEATNVNLESGDKRTGFHISEASLPVRFHFGKKPETGHVLTIDGLA
                     YVQVLLAYDLVLCDGQLFQLSGEDCKRLADLKQMVDTSGTRQLPISNEQIGFFIEKVV
                     PGLRRIGDVQLDDTVMDQLVKSPLKAKLYLDRVNNRLLASLEFQYDHIVLNPVTERDL
                     PAGSVLVRDLEKEEA"
BASE COUNT          601 a          411 c          578 g          494 t
ORIGIN      
        1 actcggcgga actgttggag ttggcggacc aatgtctgta tgaggtgaaa cggacagggc
       61 ggaatcgtac ggctgattgt gctgcgctga aaataaatac atgaggagga tgaaatcatg
      121 cagtattcga aaacgaaaat ggaacagctt gtgaaaaaca atgcggaatt gacacagcgg
      181 ctgaagcaga tcatgaaaga acatgaattg gaaaagagct ttgcattgaa ggcgttgtac
      241 cacgccgaag tgaaggaaag cggcaggttc atgaaggatt atcaggagct ttgaacgatc
      301 gattaacagg cagagacacg tccagcagca tcggcgtgcc tctgcttttt tgttttgcga
      361 taaaatcggt agagtagtgt ggatttaaaa tcgaattcat tgaaaaatag gaacatttgt
      421 tttatagtat tggaaggagg tggaagttcg gttgcctttt tgaaaaagag ggaacaccat
      481 ataactgaag ggggggagtc aaatgcaaaa tcggatagag gaattggagg aggcacgctt
      541 gaaagggttg aatcaaggca tccggcaaga gaaagtttcc acagtgctga caatgcatgg
      601 actggctttc tccacagaaa aaatcgcctt ggtgacgagg ctcactacgc aagaaatcga
      661 agccattatt ttggaaaact ggcattgaag gttttggctt ataaatagta attcaagtga
      721 aaaaccgctt cggtcaaatg cgactggagc ggttctttaa ggtagcaaca accataataa
      781 aacacgaaaa taccttctct gagtgataag atagtatata caaaacgcct gtataggtgg
      841 agccccgaaa ggagacatac agaatgagca tccagttaac acaaaatatg attatggacc
      901 tgtgcggcac tgtttcattc aaacggggcg agatgtttaa ccgtgcaggg aaagtgacga
      961 ttgaacaata tagcgaaacc cattgcaaag cgattgtggc gggaaaggaa aatttccatg
     1021 tcaccatcga tcttactgat gaattgcccc ggacggagtg cagctgtccg gtcttgccat
     1081 tctataaata caaatgccag catgtcgcgg cagttatgat tgcgatgatt gaacggaaga
     1141 gggaagagga accggatttg acggaagggc tgctttccct tttcaacgaa cgacctcgag
     1201 cgagagggca tcagcttcat ttcgaaaata gggaagtgct cgagctgcag tttacgttga
     1261 agccggtcac tgtgggcaaa caacttatgc tcggaatcga gctggcaatt gagggaatcc
     1321 atgtagaaga catccgccaa tttttgcagg atgtcaatga aggacgggca agctcgctgg
     1381 catcagcgtt cgcttttgat tctgaaatcc attgctttga acgggaagat gaggcagtgc
     1441 tgcaacagct tatcgacatt aatcatgaag agggggcgtt cggtcatcgc tccgtcggcc
     1501 tcggtacttt gcgaatccca tcctcctcgt gggaaaagct tctacagctt ttgaatgaag
     1561 cgaccaatgt aaacctggaa agcggcgaca aaaggacagg cttccatatt tcagaagcgt
     1621 cattgccggt ccgctttcat ttcggaaaaa agcccgaaac aggacatgta ttgacgattg
     1681 atggattggc ttatgtacaa gtgttgctgg cgtatgacct cgtcttgtgt gacggtcaat
     1741 tattccaatt aagcggcgag gactgcaagc ggcttgcgga cttgaagcag atggttgata
     1801 cgtcaggcac ccgccagctg ccgatttcaa atgagcagat cggctttttc attgagaagg
     1861 ttgttcccgg cttgcggaga atcggcgacg tccagctcga tgatacggtg atggaccagc
     1921 ttgtgaaatc cccgttgaag gcgaagcttt atcttgaccg ggtgaacaac cggttgctcg
     1981 cgagccttga attccaatac gaccatatcg ttctgaaccc tgttacggag agggatttgc
     2041 cggcgggttc cgtgttggtc cgcgacttgg aaaaggagga ggcg
//
LOCUS       DASULI010000424         8626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_655444, whole
            genome shotgun sequence.
ACCESSION   DASULI010000424 DASULI010000000
VERSION     DASULI010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655444"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1622)
                     /locus_tag="VFX34_09185"
     CDS             complement(33..1622)
                     /locus_tag="VFX34_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEX5565123.1"
                     /translation="MFIGVDFVLDRKFWKNPVFSISAVVILLLVVLGAIIPGPFGKVA
                     GQLYGYTTAKFGWFYLLTVFIIIVFLIGLAISKYGAIRLGGDEERPDYPFFTWIGMLF
                     SAGFGVGLVFWGVAEPMSHYFASPMDGVEPQSQEAARIAMGYSFFHWGISQWGIFGIV
                     GLVIGFLQFRKRKDGLISTALEPLVGSSKHLKIGIDSFAVIATVMGIATSLGMGVLQM
                     SGGLEYVFHTKNTFWIQLIIIFIVFVAYMLSASTGLSKGIAYLGNFNLAMAIAMLVFF
                     FFVGPKVFILESFTLAIGDYINNFIRYSLRLQPYQGGTWVREWTIFYWAWTIAWSPFV
                     GAFVARVSKGRTIREFIAGVMIIPPVFACMWIATLGGTALYSDLNNGTQIAEAVDADV
                     TSAIFETLKHLPFTGVVSFLAIVLIFTFLITSADSATYILASMTTRGSLFPPLIVKMT
                     WGFLMSAIAAVLLYAGGLEALQSASLVSALPFTLFLILLIFSLGKLLSKEPITVRPTD
                     IRRFEKIEEEVKKKKTRRKRK"
     gene            complement(1692..2855)
                     /locus_tag="VFX34_09190"
     CDS             complement(1692..2855)
                     /locus_tag="VFX34_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5565124.1"
                     /translation="MNTKEPIWTKPFISLFLTNLSVFIVFYGLVSTLPLYAKDALSRT
                     DEEAGLLMTIFLVSAIIVRPFSGKLLDIVGKRKMLLISLFFYLICTILYYFITPFTGL
                     LALRFFQGIWFSIVTTAAGSLAADIVPKTRRGAGLGYFVMSTNLAVVVGPFIALYIIQ
                     SFSYDALFILMSVLMIIGASVALLIPQVDIKGEEIKTKGFTWNDLFEKKAMPIAFLAS
                     LIAFSYASVLSYLSIYAQEKGLLPLASFFFVVFAAVMLITRPFTGRIFDEKGPAYILI
                     PGFLLFFFGLFILSFMSSPALFLIAGAFVGLGYGALIPSMQTLAIQSTTHQRSGYATA
                     TFFTLFDSGLAVGSYVLGIIAVQMGYRNVYLLSSLLILAVLALYVLIIRKRHS"
     gene            complement(2871..3263)
                     /locus_tag="VFX34_09195"
     CDS             complement(2871..3263)
                     /locus_tag="VFX34_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5565125.1"
                     /translation="MNPLIHEIFQLSRFLSKELNQALKKHGLFAAQWTVIYCIYQHGE
                     MTLTQIWKYLNVEAPTITRTVNRLEQMGWLVTREGKDRREKYVSLTENAAVQLAEIKE
                     TIIQFENESLRRLSEEEEKVLIELLQKI"
     gene            complement(3371..4591)
                     /locus_tag="VFX34_09200"
     CDS             complement(3371..4591)
                     /locus_tag="VFX34_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5565126.1"
                     /translation="MTSSTQTQNTANPVYPIMFAIGVCHLLNDTLQAVIPAMFPVLEE
                     ERGLSFTQLGFIFFALNMVASVLQPVVGYFSDRKPMPYALPIGMTFSLIGMAGLAIAP
                     NYWLILLSVIILGLGSAVFHPEGSRVSFLAAGTKRGLSQSIYQVGGNSGQALAPLISA
                     FVLVPLGQKGAAVFIAVAALGIFILSKISAWYKRQLELDKKNNRKKVLLSSIRDLTRK
                     QIGIALALLLTIIFARSFYVTNVTSFYIFYLMDGYGLKIEQGQLYIFLFMALGAAGTF
                     FGGPWADRIGRKNVIVLSLVVPLPLCLLLPHVPLWGVGILLVAIGFFIMLSFTVTVIY
                     AQELVPTKIGTMAGLTVGLAFGMGAIGAVVIGKLMDSIGIYPTMVIASFLPFIGLVGL
                     ALPKDQKITSPNKQ"
     gene            4871..5140
                     /locus_tag="VFX34_09205"
     CDS             4871..5140
                     /locus_tag="VFX34_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565127.1"
                     /translation="MLAYGNLIINAAWTILIIFLLLNPSLVLPHLADVLAEVFQRSTD
                     DIIPQVHLIIMVIGLASIATTIIDAFSGFRKLKSENGEVNEATRE"
     gene            5277..5510
                     /locus_tag="VFX34_09210"
     CDS             5277..5510
                     /locus_tag="VFX34_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HEX5565128.1"
                     /translation="MAKIAVENPFDDVKVALEEKGHDVQMFENDENLNGYDIGVVRAI
                     SDVNVDQFDFPVVGIHGMSVQDVVDEVQQRLQR"
     gene            complement(5552..6763)
                     /locus_tag="VFX34_09215"
     CDS             complement(5552..6763)
                     /locus_tag="VFX34_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010530232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX5565129.1"
                     /translation="MKIKFNLVTQIFIAFVLAIILGSLFGSSIDFLKPFGDLFLRLIK
                     FIIAPLILSTLVVGVAGTSDPKQLGRIGVKTVAYYLATTAIAIIIGLAVAFIISPGKG
                     LSVSSDGLVAPESATQEPQSSITTILNIVPENPFTALAAGSILQIIFFALFLGLAITL
                     VGEKARPVYTFFEGFAEVMYKITGIVMKVAPLGILGLLAPVIGQYGLSVLLPLVKVIL
                     AVYIACILHAVIVYSTAVKSFAGMSPKRFFKGISPAALVAFSTCSSSGTLPVTIKNTN
                     ENLGVPNRISSFVLPLGATVNMDGTAIYQGVAVIFIAQFYNLELSFLQLLTVVLITIL
                     ASIGTAGVPGAGMIMLAMVLTSVNMPLEGIALIAGIDRVLDMMRTSVNVVGDASAAVV
                     VAGTEKVLDSE"
     gene            complement(6890..7195)
                     /locus_tag="VFX34_09220"
     CDS             complement(6890..7195)
                     /locus_tag="VFX34_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX5565130.1"
                     /translation="MENTGLKVLVHASAFFAPFLVPFIIYLVVDDSEVKRLSIQALLF
                     QLVMGALIIISAILIIFLIGIPMIIVFSLMTIIIPIIGIVKSLQGETYNYPIVGRWF"
     gene            complement(7261..8415)
                     /gene="nagZ"
                     /locus_tag="VFX34_09225"
     CDS             complement(7261..8415)
                     /gene="nagZ"
                     /locus_tag="VFX34_09225"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX5565131.1"
                     /translation="MTEKETPSFSERAQVAEPPDITISQVDVLMEEMSLEEKIGQLLI
                     VGMEGKTYGEELDRLIRQYHVGGIILLGKNISYPAGMLKLLNESKKANADYEIPLFIS
                     VDEEGGRVSRLPSSMKKLPAAEYFGRIGDEMLSYETGAYLADLLRAFGYNMNYAPVLD
                     VHSNPNNPVIGDRSFSSDPYKVADLGMAVMRGMVDNGVIPVVKHFPGHGDTHVDSHLS
                     LPVIEKTIGELQKTELIPFQRAIEEQADVIMVAHIMFPELDQKYPSSLSKKIITDLLR
                     EEMQFKGVVITDDLTMGAIVNEYTVPEAAVQSFIAGSDLLLIAGDYENQVGTIDALMA
                     AVQAGTISEERIDESVKRILKLKERYKLKDRQIEEIDLDSIERKFNELMG"
BASE COUNT         2529 a         2127 c         1801 g         2169 t
ORIGIN      
        1 acatccccac ctccacttta tttgtaaatg aattatttcc tcttccttct tgtcttcttc
       61 tttttcactt cttcttcaat cttctcaaaa cgcctaatat cagtcggtct gactgtaatc
      121 ggctccttcg ataacaattt acctaatgag aagatgagca aaatgagaaa tagcgtaaac
      181 ggcagggcgg aaacgagaga tgctgattgc aaagcttcga gacctccggc atatagcaga
      241 actgccgcaa ttgcagacat gaggaaaccc cacgtcatct tgacgataag cggcgggaac
      301 aagctgcctc tcgttgtcat actcgccaaa atataagttg cagaatccgc agaagtaatt
      361 aagaaagtaa atatgagaac gatggccaga aatgaaacga cacctgtaaa cggcaagtgc
      421 ttaagcgttt cgaaaatggc cgaagtgaca tccgcatcta ccgcttcagc aatctgcgta
      481 ccgttattca aatcgctata taacgccgtt ccccctaatg tggcaatcca catgcaggcg
      541 aatacgggcg gaataatcat cactcctgca atgaactccc ggattgtacg gcctttcgaa
      601 actcgggcaa cgaaagcacc aacgaatggc gaccaagcga ttgtccatgc ccagtaaaag
      661 atcgtccact ccctcaccca agtaccgccc tgatatggct gaagccttag actataccgg
      721 atgaaattat ttatgtagtc cccaatcgcc aatgtgaagc tttccaaaat gaacactttg
      781 ggaccgacga aaaagaagaa gacgagcatg gcgattgcca tcgccaaatt gaagttgccc
      841 aaatatgcaa ttcctttact caaccctgtc gatgcagaaa gcatatacgc aacaaagacg
      901 ataaaaatga tgattagctg aatccaaaac gtattctttg tatgaaatac atattcaagt
      961 ccgccgctca tctgcaatac acccatcccg agcgatgttg caattcccat gaccgtcgca
     1021 atgacggcaa atgagtcgat gcctattttc aaatgcttac ttgaaccgac caaaggctca
     1081 agtgctgtag aaatcaggcc atctttcctt ttacggaact gaagaaaacc gatgacaagc
     1141 cctacaatgc caaaaatgcc ccattgtgat attccccaat ggaagaatga atagcccatt
     1201 gcaatacggg ccgcttcttg cgattgcggt tcgacgccat ccataggtga agcgaaataa
     1261 tggctcatcg gttccgcgac tccccaaaag acgagtccga ctccaaaccc agccgagaac
     1321 aacataccga tccatgtgaa aaatggataa tccggcctct cctcatcgcc acccaaacgg
     1381 attgcaccat atttactgat tgccaaccca attaaaaaaa cgatgataat gaaaacggtc
     1441 aatagataaa accaaccgaa cttcgccgtc gtgtacccgt ataattgtcc tgcaaccttt
     1501 ccaaaaggtc caggtatgat cgctcccaga acaacgagga gtaaaatgac gactgccgat
     1561 atggaaaata ccggattctt ccaaaacttc ctgtccaaaa caaaatcgac tcctataaac
     1621 atagttactg agtctatacc ctaaactaca gtaaggtaac gcacaacccg taaaaacttc
     1681 gggcatccct tttaactatg ccgttttcgg ataatcaaaa catataaagc aagaaccgct
     1741 aggataagaa gactggataa caaataaaca ttcctgtacc ccatttgcac tgcgataatc
     1801 cctaaaacat atgacccgac ggcaagcccc gagtcgaata acgtgaagaa cgtcgctgtc
     1861 gcatatccgc ttctctgatg ggtagtcgat tggatggcaa gcgtctgcat gctagggatg
     1921 agcgctccat agccgagccc gacaaacgct cccgctataa gaaataaagc cggagaactc
     1981 atgaaggaga ggatgaacaa accgaagaag aatagcagga atccgggaat caaaatataa
     2041 gccggccctt tttcgtcaaa aatccttcct gtgaaaggcc gggtaatgag catgaccgct
     2101 gcaaagacga caaaaaagaa actggcgagc ggcaataagc ctttctcctg tgcatagatc
     2161 gataaatagg acaggacact ggcatacgaa aatgcgatca aactagccaa aaatgcaatc
     2221 ggcatcgcct tcttttcaaa caggtcattc catgtaaagc ctttcgtttt aatttcttcc
     2281 ccctttatgt ccacttgcgg gatgagcaat gccacggatg ctccaatgat catcaggaca
     2341 ctcatcaaaa tgaacaatgc atcataggaa aacgattgga tgatgtaaag ggcaatgaac
     2401 ggaccgacaa cgacggccaa atttgtcgac atgacaaaat agccaagccc tgctccgcgc
     2461 cgcgtttttg gaacgatatc cgcagccagc gaccctgctg ccgttgtgac gatgctgaac
     2521 caaatacctt ggaagaaacg caaggcaagc aaaccagtaa atggtgtgat gaaataataa
     2581 agaatcgtgc aaataagata aaagaacaag ctgattaaaa gcattttccg tttacctact
     2641 atatccagca gttttccgga aaacggcctg acaatgatgg cggacactaa aaagattgtc
     2701 atcaataaac ctgcctcttc atccgtccgg ctcaatgcat ctttcgcata aagtggcaat
     2761 gtcgatacca agccgtaaaa cacgatgaaa acggacaagt ttgtcaggaa taggctgatg
     2821 aacggcttcg tccaaatcgg ttcttttgta ttcataaaat cattctcctt ttatattttt
     2881 tgcagcaatt cgatgaggac cttttcctcc tcttcagata atcttcgcaa gctttcattt
     2941 tcaaactgaa taatcgtctc ttttatttca gccagttgca cagctgcatt ttcagtcaag
     3001 ctgacatatt tttcgcgcct gtccttccct tcccgggtca caagccagcc catctgctcc
     3061 aatcgattga ctgtccgtgt aattgtcggc gcctctacat tcaagtattt ccaaatctgc
     3121 gtcaatgtca tctcgccgtg ttgatagata cagtaaataa ccgtccattg ggcggcaaac
     3181 aagccatgtt ttttcaatgc ttgattcagc tctttactca aaaaacgtga tagttggaaa
     3241 atctcatgaa ttaaaggatt catctcaaca cctctttatt tacttaggta actaataaag
     3301 tatacgcctg tccgaccttc ttgtaaagac atcaaaaagg ctgccaactg attggcagcc
     3361 tatactttca tcattgttta ttcggtgaag ttattttctg atccttcggc agcgccagtc
     3421 cgactaagcc gatgaatggc aagaaagagg caatgaccat tgtcgggtag atgccgatac
     3481 tatccatcaa cttcccgata actaccgccc ctatcgctcc cattccaaat gccaaaccga
     3541 cggtcaaacc ggccatcgtc ccgattttgg tcggtacgag ctcttgcgca taaatgacag
     3601 tgacagtgaa gctaagcatg atgaagaaac cgattgcaac caatagtatc cctacacccc
     3661 aaagcggtac atgaggaagt agaaggcaga gcggcaaggg tactactaac gatagcacaa
     3721 tgacgttttt ccttcctatc cggtctgccc agggtcctcc gaaaaatgtg cctgctgcgc
     3781 caagcgccat gaaaaggaat atgtatagct gaccttgctc aattttcagt ccatagccat
     3841 ccatcaagta aaagatgtag aaactagtca cattcgtgac gtaaaaagat ctggcgaaaa
     3901 tgatggtcaa taataatgcg agggcgattc ctatttgttt tcttgtcaaa tccctgattg
     3961 aagaaagcaa aaccttttta cgattattct tcttatccaa ttccaactga cgtttatacc
     4021 aagcggaaat tttggataat atgaaaatcc ctagagccgc tactgcaatg aaaacggctg
     4081 cccccttttg acctaacggc actaagacaa atgcgctgat cagcggtgcc aatgcttgtc
     4141 ccgaattccc cccgacttgg tagatcgact gggacagccc tcttttcgtt ccagccgcca
     4201 agaaggagac gcgcgagcct tccgggtgga acaccgctga gccaagcccc aatatgatga
     4261 ccgacaggag aatgagccaa tagttcggcg cgatcgccaa acccgccatt ccgataagtg
     4321 aaaatgtcat gccaatagga agtgcatacg gcatcggctt tcgatcactg aaatagccga
     4381 cgacgggctg aagaacggat gcaaccatat tcaatgcaaa aaagatgaat ccaagttgtg
     4441 taaaacttaa tcctctttct tcttccaaca cgggaaacat cgcaggtata accgcttgaa
     4501 gtgtatcatt cagcaagtgg caaaccccga tggcaaacat gatcgggtag accggattgg
     4561 ctgtgttctg tgtttgtgta ctagacgtca tcgaaatccc cacgcttttt aaataaattt
     4621 gaacaatatg tccagtatac agcaatccga agagcgaatg ttgagaatcc aggaaattcg
     4681 tatttcccgt cgttttaaat ccttggattt ccgggtagat tacatagtac tagcattcga
     4741 aaggattgaa atcgaaatgg cagcctttgg caatgtttct ttgatgactt tgtttccggt
     4801 aaccctcttg ttgctcacta tggaaatcgt cctggcctcc tacaaatcct tttctccaaa
     4861 atggacagca atgctggcgt acggcaatct catcatcaat gccgcttgga caatcctgat
     4921 tatcttcctt cttctcaatc cgtcgcttgt attgccccat ttggccgacg ttctagcgga
     4981 agtgttccaa cgatcgaccg acgatatcat tccgcaagtc catttgatta tcatggtaat
     5041 tggactcgct tccatcgcta caacgatcat cgacgcattc tccggtttta gaaaactgaa
     5101 gagcgaaaat ggcgaagtga atgaagcaac gcgggaatga agggtgctgt ttgcgtgaga
     5161 aatggagccg ttgtggaaat aatggagccc ttgtgcaaaa attgcttcgt gtttagtaga
     5221 tttgattccg ggaaaacaaa gaacggcatc actttactaa atcggaggtg tttaatatgg
     5281 cgaagatcgc tgtcgaaaac ccatttgacg atgtgaaagt ggcattggag gaaaaagggc
     5341 atgatgtaca gatgtttgaa aacgacgaga atctgaatgg ctatgatatc ggagtggtac
     5401 gtgcaatcag tgatgtaaac gtcgatcaat tcgatttccc tgtcgtcggt attcacggaa
     5461 tgtccgttca ggatgtcgtg gatgaagtcc aacagcgttt acaacgataa taaaagaccg
     5521 ggatcctctt tcggattccg gtcttccatt ttcattcact atccaatact ttctccgtgc
     5581 ccgccacaac gactgcggcg gaagcatccc caacgacatt caccgatgtc ctcatcatat
     5641 cgagcaccct gtcgatgccg gcaatcagcg caattccttc cagcggcata ttcacagagg
     5701 tcaaaaccat cgcgagcatg atcatgcctg cccccggaac tccggctgtc ccgattgatg
     5761 ccaaaatggt aatgaggaca accgtcagca actgaaggaa cgataattcc aagttataga
     5821 attgtgcgat gaaaatgacg gcaacccctt ggtagatcgc cgtgccgtcc atgttgactg
     5881 ttgcacccag cggaaggacg aagctcgata tcctgttcgg aacccccagg ttttcgttcg
     5941 tatttttgat tgttacagga agcgtccccg agctgctgca cgtactgaat gcgacaagcg
     6001 ctgctgggga aatccctttg aagaaccttt tcggactcat cccggcaaat gacttgaccg
     6061 ctgtcgaata aacgattact gcatgtagga tacaggcgat gtaaacagcc aagatcactt
     6121 tgacgagcgg caacaggacg gaaagcccat attgtccgat aaccggagca agtagaccta
     6181 aaataccgag cggtgccact ttcatgacaa tcccggttat tttgtacatg acttcggcaa
     6241 aaccttcaaa aaacgtatag acgggacggg ccttttcacc gactaacgtt atcgccagcc
     6301 caaggaataa tgcgaagaag atgatctgca aaatgctgcc ggccgccaag gcggtgaacg
     6361 gattttcagg cacgatattc aagattgtcg taattgagct ttgcggttcc tgcgtcgccg
     6421 attccggagc gaccaatcca tcggatgaaa ccgataaacc tttccccggt gaaattatga
     6481 atgcgacagc aagaccgatg atgatggcaa tcgctgtagt ggccaagtaa taggcgaccg
     6541 tcttgacacc gatcctccca agctgctttg gatcggaagt gcctgcaacc cctacgacca
     6601 atgtggataa aatgagtggc gcaatgatga atttaattaa ccttaaaaat aaatccccga
     6661 acggcttcag aaaatcaatg gaactgccga acaaactgcc taaaataatg gctaacacaa
     6721 aggcgataaa aatttgcgtc acgagattga atttgatctt catacctctc ccctttcatg
     6781 aatttgtacg ttcccttgct tattccaaca tatacatttc ggaagcataa catgaatgaa
     6841 aaagccaata aaaaagcccc ctgaccgctt tcgcgaatag gggggccgat cagaaccatc
     6901 tgccgacaat cggatagttg tatgtttctc cttgcaacga tttcacaatt ccaatgattg
     6961 gaatgatgat tgtcatcaaa ctgaagacga tgatcatcgg tatgccgatc aaaaaaatga
     7021 tcaaaatcgc cgaaatgata atcaatgcgc ccatcactaa ttggaacaaa agcgcctgta
     7081 tagataatct cttcacctct gaatcatcca caactaaata gatgatgaac gggacaagga
     7141 atggggcgaa aaatgcgctt gcatgaacta gcacttttaa acctgtgttt tccatatcgt
     7201 gtacctccta gttttctact gtactttatt ttacggataa caaaggagga agtttcactt
     7261 tcaccccatt aattcattga atttgcgttc gatagaatcc aggtcaatct cttcaatttg
     7321 acgatccttc agtttataac gttctttcag ctttaaaatc cgcttgacgc tctcatcgat
     7381 acgttcctct gaaatcgttc ctgcttgcac tgcagccatc aatgcatcga ttgtaccgac
     7441 ctggttttca taatctcccg caatcagcaa tagatcactg cctgcaatga aggactggac
     7501 ggccgcttcc ggtaccgtat actcattgac gattgcaccc atcgtcaagt catcggtaat
     7561 gacgacgcct ttaaattgca tttcttcgcg cagcagatcc gtaatgatct ttttcgatag
     7621 ggaggatgga tatttctgat caagctctgg gaacataatg tgggccacca taatgacatc
     7681 cgcttgttct tcaatcgccc gctgaaaagg gatcaactcg gttttctgca gctccccgat
     7741 cgtcttctca atgactggaa gcgacaagtg ggaatctaca tgtgtgtccc cgtgacctgg
     7801 aaaatgtttg acgacgggaa tgacaccatt gtccaccatg cctcgcatga cggccatgcc
     7861 caaatcggcg accttatacg gatcggatga gaatgaccgg tctccgatga caggattatt
     7921 cggattgcta tggacatcca aaacgggtgc atagttcatg ttatagccga atgctcgcaa
     7981 aagatccgca agatatgcac cagtttcata agaaagcatt tcatcaccga ttcttccgaa
     8041 gtattccgcc gcaggcagtt ttttcatact cgaaggcagt cgtgatacac gcccaccttc
     8101 ttcatcgacg gaaatgaaca gcggaatttc ataatcggcg ttcgccttct tgctttcatt
     8161 caatagcttc aacattccgg cgggatacga aatattcttg cctaataata tgatgccccc
     8221 gacatgatat tgacggataa gacgatccag ctcttcgccg tatgtcttcc cctccatccc
     8281 gacgatcagc agctgtccaa ttttttcttc cagcgacatc tcctccatca atacatcaac
     8341 ttgggaaatt gtgatgtccg gtggttcagc cacttgggcc ctttcactga atgaaggagt
     8401 ttctttttca gtcatttgca ctgcccgact gcatcccgtc aaaagcatga ggatgcatat
     8461 aatgaatcct atatagcgga acaatggact tcgccaattc aactccaaat ttcttttgcc
     8521 ctatcaaggg agtcaagtga aatacggcct ttcaccattg gaggggggca atgtctatta
     8581 tgctcagcga tctttttatt cacttcatcc acacgctttt gcagat
//
LOCUS       DASULI010000425         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1504712,
            whole genome shotgun sequence.
ACCESSION   DASULI010000425 DASULI010000000
VERSION     DASULI010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504712"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..742)
                     /locus_tag="VFX34_09230"
     CDS             complement(65..742)
                     /locus_tag="VFX34_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5565132.1"
                     /translation="MKPYTILIIDDEPQMRNLVRTFLDKDGYVVEEATDGLDALSKVY
                     NLQPHLCIVDVMMPYMNGFEFAKELKKTTNIPIIFLSARGEEWDKVEGLKIGGDDYIV
                     KPFMPGELIARVESVLRRSYQQQRISPVLTVGPLVIDHLAHTATLDNKPVSLTVKEFG
                     ILETLAKKPGRVYTREQILDLVWGDHHQSTDRTIDTHIKTLRLKLGEAGSMIETVWGI
                     GYKLEVK"
     gene            902..1654
                     /locus_tag="VFX34_09235"
     CDS             902..1654
                     /locus_tag="VFX34_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX5565133.1"
                     /translation="MKKRFKMLGMLIGLGLLAACGKEADDGSAVEVLPLDVELTVTEQ
                     AEVGDTVKMEALVTYGDEKVSDADKVVYEVWEEGKKDDSVMIDSVNEKDGTYTAETSF
                     DHDGLFHIQVHVDAKNLHTMPVKQVTIGEGAHAETEHAEGEEDGHDHGHAEGFALHFV
                     KPEHVMMGEETELMTHLQIDGEPLEKAEVRYEISSDVLGEEHLWIDAAETEKGEYIAV
                     HPFKEAGTYTVKIHVENADGLHEHEEFTIEVK"
     gene            1787..2119
                     /locus_tag="VFX34_09240"
     CDS             1787..2119
                     /locus_tag="VFX34_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX5565134.1"
                     /translation="MNGLPEKTCSIERLVTIPADVERVLKGEKTATRRNGVYAYPDEI
                     MVLDGKEFKIDKLYKQTLGEMTDDDAKQEGFANMEEYKESILALHPKMPWLPTMSVWV
                     HEFSPVAK"
     gene            2126..2563
                     /locus_tag="VFX34_09245"
     CDS             2126..2563
                     /locus_tag="VFX34_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565135.1"
                     /translation="MKYMYGTIVFVHVVSAVLAIGPLFLLLPTIKRLRNVDIPTENAY
                     LAIIKVIIRLVMHAGHMLVLSGALLLLTGPWPWNTSWVIATLGIMLLSGFFLSSGFTK
                     VLRKYYKHSSKGQILTVLTRTTWIYIGLMLIMLWLMVQKPMLW"
     gene            2697..3545
                     /locus_tag="VFX34_09250"
     CDS             2697..3545
                     /locus_tag="VFX34_09250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5565136.1"
                     /translation="MEFVQYDDAKLFGEIAEPITAKNEDMFSLFFGILQAIKAGKYEN
                     PFMAAVSDGEDVLALLQMTPPHPLNLIIVDEGRKDETIDFIIRELRGNSIAVPSVISL
                     KEWALLFADKWEAQTGEKQKLLMDQGLYRLDEVDETLEMSPGSWRFASIEDAPLIEKW
                     FALFEEDTNLDRTPPETIRERVAAFLEAREVFLWEDEGKVVSMMKKSRPTHNSITVSL
                     VFTPREERKKGYARTMVAQGSKELLKDYGFCVLYTDMMNPTSNKIYKEIGYKHIADSI
                     HIGFEK"
     gene            complement(3579..>4038)
                     /locus_tag="VFX34_09255"
     CDS             complement(3579..>4038)
                     /locus_tag="VFX34_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5565137.1"
                     /translation="NMAGWRVAFAVGNRQMIQAINKLQHHLFISLFPAVQHAAIAALT
                     EDQACVRDLTAMYEGRRNVLIGECKRIGWDVEAPKASFFAWLPVPPGYTSEGFADILL
                     QQAGVVVAPGHGFGTHGEGYVRVGLLASEDRIAEAVRRIEKLGLFSVSEA"
BASE COUNT         1152 a          853 c         1006 g         1027 t
ORIGIN      
        1 attgtaaaag caaggctgga taacaagaca attaatattt ttttgctcaa tgtgagcttt
       61 ttcatcattt cacctcaagc ttatacccga tgccccaaac tgtttcgatc atgcttccgg
      121 cttcccccaa tttgagtcgc agtgttttaa tatgcgtatc aatcgtccgg tcggtgcttt
      181 ggtgatgatc gccccagaca aggtccaaaa tctgttccct tgtatagaca cggcccggtt
      241 tttttgcgag ggtttccagg atcccgaatt ccttcaccgt caatgaaacc ggtttattat
      301 cgagcgttgc cgtatgtgca agatgatcga ttacgagagg accgactgtc agcaccggtg
      361 atatgcgctg ctgttggtat gatcttctta gaacggattc aaccctggca atcagctcgc
      421 caggcatgaa cggcttgacg atataatcat caccgccgat tttaaggcct tcaaccttat
      481 cccattcctc tcctctggcg gataaaaaga tgatagggat attggtcgtc ttttttaatt
      541 ctttggcaaa ttcaaatcca ttcatatacg gcatcatgac atcgacgatg cataaatggg
      601 gctgcagatt gtacactttt gacaatgcat ccagtccatc cgtcgcctct tcgactacat
      661 aaccgtcttt gtcaaggaaa gtgcgcacca aatttctcat ttgcggttca tcatcaatta
      721 ttaagatcgt atatggcttc atccgaatat cctcatttca cagaaataat caattatact
      781 atactcaaca gttgtgaaat acttatgaaa atgggcactt cataattact tcgcaatcac
      841 ttgatactat catatcggaa tatatatgct cgtcaataaa gaagcagtgg agggtatcat
      901 catgaaaaaa agattcaaaa tgctcgggat gctcatcggg ttgggacttt tagccgcttg
      961 cggaaaggaa gcggatgatg gctcggcagt agaagtgctg cctttggatg tggaattgac
     1021 tgtgaccgaa caggcggaag tcggcgatac agtgaagatg gaagcactcg ttacatacgg
     1081 cgatgaaaaa gtgagcgatg ccgataaagt cgtctacgaa gtttgggagg aagggaagaa
     1141 agatgactcg gtcatgatcg actccgtcaa tgaaaaggat ggcacctata cggctgaaac
     1201 atcgtttgac catgacggcc tgttccatat ccaagtccac gtggatgcga aaaatctaca
     1261 tacgatgccg gtgaaacaag tgacaatcgg tgaaggcgca catgcagaaa ccgaacatgc
     1321 cgaaggggag gaagatggcc atgaccatgg tcatgcggaa ggttttgcgc tacatttcgt
     1381 aaaaccggaa catgtaatga tgggtgaaga gaccgagttg atgacccatt tacagataga
     1441 tggtgagccg cttgaaaaag cggaggtccg ttatgaaatt tcaagcgacg tcctcgggga
     1501 agagcatctt tggattgatg cagcagaaac tgaaaaaggc gaatatattg cggttcatcc
     1561 attcaaagaa gcagggacat acacggtgaa gattcacgtt gaaaatgcag atggcttgca
     1621 tgagcatgaa gaattcacga ttgaagtgaa atagatccga aagtaaggaa tgtggctgta
     1681 tcgtgaattc cggcaatgaa tccgagtgtt ttaccatagt tacatctgga attatcgata
     1741 gggaatcgat atgatagatg tagattcaga agaggagcga tgaattatga atgggttacc
     1801 ggaaaagacg tgttcgattg aacggcttgt caccattcca gcagatgtcg agagggtatt
     1861 gaaaggtgaa aagacagcga cgagacgaaa cggtgtctac gcatacccgg atgaaatcat
     1921 ggtgttagac gggaaagagt tcaaaatcga taagctctat aagcaaacac tcggtgaaat
     1981 gacagatgat gacgctaaac aggaagggtt tgcaaacatg gaggaataca aggaatcgat
     2041 tcttgcttta cacccgaaga tgccttggct tccgacaatg agcgtgtggg ttcacgaatt
     2101 cagcccagtt gcaaagtaag atggcatgaa atacatgtat gggacaattg tatttgtcca
     2161 cgtcgtttcc gcagtccttg caatcggccc cttattccta ttgttgccga caatcaaaag
     2221 gctgcggaac gtcgatattc caaccgaaaa tgcatacttg gcgatcatca aggtcattat
     2281 caggctcgtc atgcacgccg gtcatatgct cgtcctgtct ggcgcattgc tgctgctcac
     2341 aggtccgtgg ccttggaata cgtcttgggt gatcgcgacc ctcggaatca tgctgttatc
     2401 cggctttttc ttatcaagcg ggtttacgaa agtcctcaga aaatattaca agcatagcag
     2461 caaggggcag attttgaccg ttttaacgag gacaacctgg atatatatcg gattgatgct
     2521 catcatgctt tggctaatgg tgcaaaagcc gatgctttgg tgaaataata acggattaca
     2581 taaaatgagg atgtgcaaag gatcgatgca tatccttttt aaattctctt aaaaagaatc
     2641 gtgacaattc ggttttttga gtatataatt ctcggaaacg aaaaggggag atcgggatgg
     2701 agttcgttca atatgatgac gcaaagcttt ttggagaaat cgctgaaccg attacagcga
     2761 aaaatgaaga tatgttcagt cttttcttcg gcatcctgca ggcgataaaa gcgggaaaat
     2821 atgaaaatcc ttttatggcg gccgtgtcgg atggggaaga tgtgcttgca ctattacaga
     2881 tgacgccgcc gcatccattg aatcttatca tcgtggatga aggcaggaag gatgaaacga
     2941 ttgatttcat catacgggaa ctgcgtggga attcgattgc ggttccgtca gtcatcagtt
     3001 taaaggaatg ggctcttttg tttgcagaca agtgggaagc gcaaacgggg gagaagcaaa
     3061 agctgcttat ggatcagggg ctttatcggt tggatgaggt tgacgagaca cttgaaatga
     3121 gtccgggaag ctggaggttt gcttcgattg aggatgcccc gctaattgaa aagtggtttg
     3181 cattgttcga ggaggacacg aatcttgata gaaccccgcc ggagaccatt agagaaaggg
     3241 tagccgcatt ccttgaagcc cgggaagttt tcctatggga agatgaaggg aaagtcgtct
     3301 cgatgatgaa gaaatcaagg ccgactcata atagcatcac cgtttccctt gtttttacgc
     3361 ctcgagagga aaggaagaag gggtatgccc gcacgatggt cgcgcaaggt tcgaaggagt
     3421 tattgaagga ctatggtttc tgcgttctct atacggacat gatgaatcct acgtcgaata
     3481 agatttacaa ggaaatcggc tacaaacata ttgccgattc gattcatatt ggttttgaga
     3541 agtaagacaa aggccgcctc cccaggcggc ctttcctatc aagcttcact cactgaaaat
     3601 aaccccagct tttcaattct acgaacggcc tccgcaatac gatcttcgct cgccagcagg
     3661 ccgactcgta cataaccttc accatgcgtt ccaaatccat gccctggagc gacgacgact
     3721 cccgcttgct gaagaagaat atccgcaaat ccctcgctcg tatagcccgg gggaacaggc
     3781 agccaagcga agaaagaagc tttcggcgct tccacatccc atccgatcct tttgcactca
     3841 ccaatgagca catttcgacg accttcatac atagcagtca aatcacgcac acatgcctga
     3901 tcttctgtca aagcggcaat cgcagcgtgt tgaactgccg gaaaaagact gatgaacaag
     3961 tggtgctgca gtttattgat ggcctggatc atttggcggt ttccaacggc gaaggcaacc
     4021 ctccagccgg ccatgttg
//
LOCUS       DASULI010000426         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1590360,
            whole genome shotgun sequence.
ACCESSION   DASULI010000426 DASULI010000000
VERSION     DASULI010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590360"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFX34_09260"
     CDS             complement(<1..881)
                     /locus_tag="VFX34_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5565138.1"
                     /translation="MKKKFGLRKKLVLFVTVLAVVTYSTSALFINYIRPEFFPDVQPF
                     VFEIITYALGILWSGILAALFSTILTKPLQNLEKAAIEVADGKIGTDIALPKSSDEIR
                     SVAEAFQQLVVNLRTIVTEIETNFEKTAQTVDQLSGETSSAALQADAVASTIMEISSG
                     AEASAVAIQETAEAIEDVRTLAAEVSSRAEDSSEQSKEMLAELERTTEVFRTLVKGIR
                     DMSDQSEQSLGTIRELDQNAQKIGEIVQLVGSIAGQTNLLALNASIEAARAGEHGKGF
                     AVVAEEVRVLADESAKAV"
     gene            complement(878..1324)
                     /locus_tag="VFX34_09265"
     CDS             complement(878..1324)
                     /locus_tag="VFX34_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HEX5565139.1"
                     /translation="MNIYFICTGNTCRSPMAEAILRSKAIEGVTVRSAGVNAADGLPI
                     ATNAKTLIEGADMPYTPVSNAVTEEDLEWADLVLTMTMSHKAMLLHSFPEMEAKTFTL
                     KEFAAPGEFGDVHDPYGGSLATYRTTFDELGRLIEQLKMKLTEDEA"
     gene            complement(1381..1884)
                     /locus_tag="VFX34_09270"
     CDS             complement(1381..1884)
                     /locus_tag="VFX34_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HEX5565140.1"
                     /translation="MLEIFAASVTTIDVVVVLSLMQLRKGRLVLALWITFLNMVLPLA
                     GFVMGDWSAHLFSVWSHLLSGVLLALIGIHMLLQDDDNDLVRRQLHPMIIALAVSLDT
                     FSISVSFGMLQLNKILFILASGFLSFIFSCLALYFGRFLSIKNGKVLRVIAGLALLAM
                     GILSCFR"
     gene            complement(1983..>2325)
                     /locus_tag="VFX34_09275"
     CDS             complement(1983..>2325)
                     /locus_tag="VFX34_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HEX5565141.1"
                     /translation="GMKYMHYAPESPLFVIEPDEAVIREAVRQLHSEGKKVAVIGPDE
                     FGIAEADWYFPVGPLHDNGAMAVNLYGAIRQCDMTDADLILAAGTDQEGVGAAVMNRL
                     LKAADGKRFNG"
BASE COUNT          567 a          698 c          522 g          538 t
ORIGIN      
        1 accgctttcg cactttcatc cgcaagcaca cggacttcct ccgcgacgac cgcgaagcct
       61 tttccatgct ccccggcacg tgccgcttcg atggatgcgt tcagcgcaag caaattcgtc
      121 tgtccggcga tgctgccgac gagctggacg atttcgccga tcttctgcgc attttggtca
      181 agttcgcgga ttgtgccaag cgactgctcg ctctgatccg acatgtcgcg gatccctttg
      241 acgagcgtcc ggaacacttc tgtcgtacgc tcgagttccg cgagcatctc tttcgactgt
      301 tcggacgaat cttccgcacg gctgctcact tccgctgcaa gcgtccgcac atcctcgatc
      361 gcttcagccg tttcctggat tgcgaccgcc gaagcttccg cacccgagga gatttccatg
      421 atcgtcgaag cgaccgcatc ggcttgaagc gccgcactgc ttgtctcacc cgacaactgg
      481 tcgaccgtct gcgccgtctt ctcgaaattc gtctcgattt ccgtcacgat cgtccttaaa
      541 ttgacgacga gctgctggaa cgcctccgcg acggagcgga tttcgtcgga tgacttcggc
      601 aatgcgatgt ccgtaccgat cttgccatcc gccacttcga tcgccgcctt ctccaagttt
      661 tgaagcggct tcgtcaaaat cgtgctgaac aaggcagcca aaatgcccga ccataatatt
      721 cctaacgcgt atgtaatgat ttcaaataca aacggctgga cgtcaggaaa gaactccgga
      781 cgtatgtaat taatgaacaa ggcgcttgtc gaatacgtga caaccgcaag caccgtaacg
      841 aataaaacca atttcttccg caaaccgaac ttcttcttca tgcttcatcc tccgtgagct
      901 tcatcttcaa ctgctcgatc aacctaccca attcatcgaa tgtcgtcctg taggtggcca
      961 agctgccgcc gtacggatcg tgcacatccc cgaattcgcc gggtgcagcg aactccttca
     1021 acgtaaatgt cttcgcttcc atttccggga atgaatgcag cagcatcgcc ttatgcgaca
     1081 tcgtcatcgt caacacgaga tccgcccact cgagatcctc ttccgttacc gcgttcgaca
     1141 ccggcgtgta aggcatgtcc gccccttcga tcagcgtctt ggcgttcgtc gcaatcggca
     1201 acccatcggc cgcattgacg ccagccgacc ggaccgtgac gccttcaatc gctttcgaac
     1261 ggagaatcgc ctccgccatc gggctcctgc atgtattccc tgtacaaata aaataaatat
     1321 tcattcatga tcacctacct ttagaatacc tgatagccgt gaataaagaa atagataatt
     1381 tcatctaaaa catgacaata tccccatggc caataaagcg agtcctgcaa taacgcggag
     1441 caccttgccg ttcttgatgc tcaaaaaccg cccgaaataa agagcgagac aggaaaatat
     1501 aaaagaaagg aatccggaag ccaagatgaa cagtatttta ttcaactgga gcatgccgaa
     1561 agagacactg atcgaaaaag tgtccaagct tacggcaagc gcaatgatca taggatgaag
     1621 ctgccgtctg accagatcgt tatcatcgtc ctgcagaagc atatggatgc cgatcaatgc
     1681 tagcaggaca cccgacagca aatggctcca cactgaaaaa aggtgagcgg accaatcccc
     1741 catgacgaaa cccgcgagcg gcagcaccat gttcaggaat gttatccaca gtgcaaggac
     1801 gagccttcct ttccgtaatt gcatcaacga taatacaacg accacatcga ttgtcgtaac
     1861 acttgcagcg aatatttcca acaatgaatt gacctcccaa cttccgcatc catccatctt
     1921 atgcgtacgt ttaggaggtc attcatatcc taatatctgt tggtcatata caaaaaatcc
     1981 tatcatccgt tgaaccgttt tccgtccgcc gctttcagga gtcgattcat cactgcggca
     2041 ccgacgcctt cctgatccgt ccccgcggct aggatgagat cggcgtccgt catatcgcat
     2101 tgccggatcg caccatacag attcacagcc atcgccccgt tgtcatggag cggaccgacc
     2161 gggaaatacc agtcggcttc ggcaatcccg aattcgtcag gaccgatgac cgcaaccttt
     2221 ttcccttcgg aatgaagttg cctgaccgcc tcccggatta ctgcttcgtc cggctcaatg
     2281 acgaatagcg gcgattcggg ggcgtaatgc atatatttca tgccg
//
LOCUS       DASULI010000427         4879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_227799, whole
            genome shotgun sequence.
ACCESSION   DASULI010000427 DASULI010000000
VERSION     DASULI010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227799"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFX34_09280"
     CDS             <1..661
                     /locus_tag="VFX34_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5565142.1"
                     /translation="TGGKLVEGYGLTETSPVAIANFVWEKRRVKGSIGVPWPDTDACI
                     LGPESSEPLPNGEVGEIAVKGPQVMKGYWNRPEETAETFRDGWLLTGDLGYMDDEGHF
                     YVVDRKKDMIIASGFNIYPREIEEVLYEHDAIQECVVAGVPDPYRGETVKAYIVLKEG
                     KSVTEEELNTYCREHLASFKVPRQYEFRKELPKTAVGKILRRSLIDEEKKKMEQELQT
                     S"
     gene            750..1331
                     /locus_tag="VFX34_09285"
     CDS             750..1331
                     /locus_tag="VFX34_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5565143.1"
                     /translation="MKRDKPKYKQIVDAAVIVIAENGYHQAQVSKIAKEAGVADGTIY
                     LYFKNKEDILISVFREKMGVFVDNVKEILKKDISATEMLAKMIENHFSLLNGDRHLAI
                     VTQLELRQSNKELRYRINEVLKEYLTLLDTILKEGIEKGEFDKQIDIRLARQMVFGTI
                     DETITSWVMNDQKYDLMKLAPEVHRLLLNGMKA"
     gene            1385..2158
                     /locus_tag="VFX34_09290"
     CDS             1385..2158
                     /locus_tag="VFX34_09290"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5565144.1"
                     /translation="MEFLKIAIEDGVATVAIDRPPANALSSGLIREVDQMLDKVENDD
                     SVRVIVLHGEGKFFSAGADIKEFTTVTSGEEFSQLASAGQDVFERMESFKKPIIAAIH
                     GAALGGGLELAMGCHIRFVTERAKLGLPELQLGLIPGFAGTQRLPRYVGMAKAAEMLL
                     TSDPITGVEAVNWGLANRAFPDDELLPKTLEIAKKIAKKSPIAMKAALRMLQYTKTDS
                     FYEGVKAEADSFGEVFVSADAKEGIQAFIEKREPEFKGN"
     gene            2181..2954
                     /locus_tag="VFX34_09295"
     CDS             2181..2954
                     /locus_tag="VFX34_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5565145.1"
                     /translation="MNIYALVKRTFDTEERIAVSNGKIVEDGAEFIINPYDEYAIEEA
                     IQVRDEHGGEVTVVTIGDEEAEKQLRTALAMGADKAVLINTEDDLDEMDEFTAAKIIA
                     EYLKDKDADLILAGNVAIDGGSGQVGPRVAELLGINYVTTITNLEIEGTSVKIIRDVE
                     GDSETIETSLPLLVTAQQGLNEPRYPSLPGIMKAKKKPLDELELDDLDLDEDDVEAKT
                     ETVEIYLPPQKAAGRVLEGDLSDQVKELVDLLNKEAKVL"
     gene            3158..4132
                     /locus_tag="VFX34_09300"
     CDS             3158..4132
                     /locus_tag="VFX34_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX5565146.1"
                     /translation="MSKKVVVLGEAREGALRNVSFEAIAAAKKVAGGGEIVGLLFGDS
                     VQTLGEEMIKYGADRVVTVEHPHLKHYTSDGFGQAFMAVYEQEKPEAIVFGHTALGKD
                     LSPKIASKLASGLISDVTSIEGEGDDAVFVRPIYSGKAFEKVKNKDGLLFISVRPNNI
                     EPLAKDESRSGDVSSVSVDIANLRSVISEVVRKSTEGVDLSEAKVVVAGGRGVKSAEG
                     FAPLEELAQLLGGAVGASRGACDADYCDYSLQIGQTGKVVTPDLYIAAGISGAIQHMA
                     GMSNSKVIVAINKDPEANIFKVADYGIVGDLFEVIPMMIEEIKKIKTN"
     gene            4256..4570
                     /gene="trxA"
                     /locus_tag="VFX34_09305"
     CDS             4256..4570
                     /gene="trxA"
                     /locus_tag="VFX34_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5565147.1"
                     /translation="MAITHATDQDFKDQTKDGLVLVDFWATWCGPCKMIAPVLEELDQ
                     DLNGKAKIVKVDVDENQATAGEFGIMSIPTLVLFKDGEIVDKVVGFQPKEQLAELIEK
                     HA"
     gene            4629..>4879
                     /locus_tag="VFX34_09310"
     CDS             4629..>4879
                     /locus_tag="VFX34_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017358241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5565148.1"
                     /translation="MNERIEHKLSLLPDLPGCYLMKDRQGTIIYVGKAKILKNRVRSY
                     FTGSHDAKTQRLVGEIEDFEYIVTSSDIEALILELNLIK"
BASE COUNT         1452 a         1010 c         1292 g         1125 t
ORIGIN      
        1 gaccggcgga aagctagtgg aaggttatgg actgacggaa acgtcgcctg tagccatcgc
       61 gaatttcgtt tgggagaaaa ggcgtgtgaa aggatctatc ggtgttcctt ggcccgacac
      121 ggatgcatgc attttaggac cggaatcttc agagccgctt ccgaatgggg aagtcgggga
      181 aatcgcagtg aaaggtcctc aagtgatgaa aggctattgg aaccggccgg aagagactgc
      241 agaaactttc cgtgatggat ggctattgac cggggattta gggtatatgg atgatgaagg
      301 ccatttctac gtggtcgaca ggaagaagga tatgatcatt gcgagcggct tcaatattta
      361 tccgcgtgaa atcgaagaag tcctatatga gcatgatgca atccaagaat gtgttgtcgc
      421 cggagttccg gatccatatc gaggggaaac ggtaaaggca tatattgttc taaaagaagg
      481 gaagtcggtg acggaagaag aattgaatac gtattgccgg gaacatttgg catcattcaa
      541 agtgccgcgt caatatgaat tcaggaaaga gctgccgaaa accgccgttg gtaaaatatt
      601 gcgcagatcc cttatcgacg aggagaaaaa gaagatggaa caggaactgc aaacttcctg
      661 acgaaagact tgacgacaat caaataaacg attaatatga aaatatgaat gaaccgtcat
      721 tcatattttc ttcttttggt ggtgaaaaag tgaaacgtga taaaccgaaa tataaacaga
      781 tagtggatgc ggccgtcatc gttattgccg aaaacgggta tcatcaggca caagtctcga
      841 aaatcgccaa ggaagcaggc gttgcggatg gaacaatcta tctttatttc aaaaataaag
      901 aggatatcct tatttccgta ttccgggaga agatgggcgt cttcgttgat aacgtaaagg
      961 agattttgaa gaaagatatc tctgcgacgg aaatgcttgc caagatgatt gaaaaccatt
     1021 tcagtttgct caatggcgac cgccacctag ccattgtaac ccagttggaa cttcggcaat
     1081 caaacaaaga gcttcggtac aggatcaatg aagtattgaa ggagtatttg actttgctcg
     1141 ataccatctt aaaagaaggg atcgaaaaag gtgagtttga caagcagatc gatattcggt
     1201 tggcacgtca aatggtgttc ggaacgatag acgaaacaat cacctcatgg gtgatgaatg
     1261 atcaaaagta tgatttgatg aaattggcac cggaagtcca tcgcttactt ttaaatggaa
     1321 tgaaagcctg aaccatacga tgaatgagtg gtgcaatagg catttcggaa agggtgggga
     1381 agaagtggag ttcttgaaaa tcgctattga agacggggtt gcgactgtcg caatcgacag
     1441 accgcctgcc aatgcattgt caagcggtct tatccgagag gttgatcaaa tgctcgacaa
     1501 agtggaaaat gatgattccg tgagggtcat cgtgcttcac ggagaaggga aattcttttc
     1561 ggcgggcgcc gatattaaag aattcacgac ggtgacgtcc ggcgaagaat tctcgcagct
     1621 tgcatccgca gggcaggacg tgttcgaacg gatggagagt ttcaaaaaac cgatcatcgc
     1681 cgcgatacac ggtgccgcac ttggcggtgg tctggaattg gcgatgggat gccatatccg
     1741 gttcgttaca gaaagggcta agcttggctt gccggaattg caattggggc ttattccagg
     1801 ttttgcgggc acgcagcgct tgccgcggta tgtcgggatg gcaaaggctg cggaaatgct
     1861 gttgacgagc gatccgatca caggcgttga ggcggtcaac tggggtctcg ccaaccgtgc
     1921 attcccggat gacgagctac ttccgaagac gttggaaatc gctaagaaaa ttgcgaagaa
     1981 aagcccgatt gccatgaagg cggctctccg catgctccaa tatacgaaaa cagactcgtt
     2041 ctacgaaggt gtgaaggcag aggccgattc attcggtgaa gttttcgtct cggcagatgc
     2101 aaaagagggc atccaagcat tcattgaaaa aagggaacca gaattcaaag ggaattaaaa
     2161 tagccaggga ggtctgcaat atgaacattt atgcattggt gaaacggaca tttgacacgg
     2221 aggagagaat cgctgtatcg aacggtaaaa tcgttgaaga cggcgctgaa ttcatcatca
     2281 atccgtacga tgaatatgca attgaggaag cgatccaagt acgtgacgaa cacggcggtg
     2341 aagtgacagt cgtcacaatc ggcgacgaag aagctgaaaa acagcttcgt acggcgcttg
     2401 ctatgggggc tgacaaggct gtccttatca ataccgaaga tgaccttgat gaaatggacg
     2461 agttcactgc agcgaaaatc attgcagaat acttgaaaga caaggatgct gatttaatcc
     2521 ttgcagggaa cgttgcgatc gatggcggct ctggacaagt cggaccacgt gttgcggaac
     2581 tattaggcat caattatgta acgacaatta caaaccttga aatcgaaggc acttcagtga
     2641 aaattatccg cgacgtcgaa ggggattccg aaacgattga aacttccttg ccattattgg
     2701 tcacggcaca acaagggttg aacgaaccac gctatccttc tttaccgggc atcatgaagg
     2761 cgaagaaaaa gccgcttgat gaactggaat tggatgatct tgatcttgac gaagatgatg
     2821 tggaagcgaa gacggaaacg gtcgaaatct atcttcctcc gcaaaaagcg gcagggcgcg
     2881 tccttgaagg cgacttgtcc gatcaggtga aagagcttgt agatcttctg aataaagaag
     2941 cgaaagtgct ttgatttaat tctgccgaat caaatcaaag cgcctccggc ggatgtcacg
     3001 gatttttaaa ggtgttagaa ggcagtctaa gttcgcgacg tcctgtcgca acgactgcat
     3061 gacccacatc ctgtgggcct caggctcgaa aaaatccggg cgcaatcacg ccgaggcgtg
     3121 attgattgtt gcatttcatt aatgggaggg actctatatg tctaaaaaag tagtagtact
     3181 tggtgaagct cgtgaaggtg cattacgtaa cgtttcattc gaagcgattg cagctgctaa
     3241 aaaagtagct ggcggcgggg aaatcgtcgg cttgctattt ggcgattcag ttcaaacatt
     3301 aggggaagaa atgatcaaat acggtgcgga ccgtgtcgta acagtggagc acccacattt
     3361 gaaacattat acatcagacg gcttcggcca agcgttcatg gctgtctatg agcaggagaa
     3421 accggaagcc atcgtctttg gtcatactgc attagggaaa gatctgtctc cgaagattgc
     3481 tagtaaacta gcatcgggat tgatttccga tgtgacatcc atcgaagggg aaggcgatga
     3541 tgctgtattc gtccgcccga tctattccgg caaggcattt gaaaaagtga agaacaaaga
     3601 cggccttcta ttcatttcgg tccgtccaaa caatatcgaa ccgctcgcaa aagacgaaag
     3661 ccgttcgggc gacgtttcct ccgtttcagt tgacattgca aacttacgct cggtcatcag
     3721 cgaagtcgta cgcaagtcga cggaaggtgt cgatctatcg gaagcgaaag tcgttgtcgc
     3781 tggaggccgc ggtgtgaaga gtgcagaagg cttcgcacca cttgaagaat tggctcagtt
     3841 gcttggcggt gcagtcggtg catcccgcgg agcatgtgac gccgactact gcgactattc
     3901 cttgcaaatc ggacagacag gtaaagtcgt aacgcctgac ctttacatcg cagctggaat
     3961 ctcaggcgca atccagcata tggctggaat gtccaactca aaagtgatcg tcgccatcaa
     4021 taaagatcct gaagcaaaca ttttcaaagt ggcggactac ggcatcgtcg gagacctatt
     4081 cgaagttatc ccgatgatga tcgaagaaat caagaaaatc aaaacaaact aatcagtctg
     4141 aggctcctgc ataagcggga gcctttcgta cgctttaagc agatgtttcg ctcggctata
     4201 tttgacatgc tataatgcaa tcatagttgg atttactaaa ggaggaaata aaaaaatggc
     4261 tattactcat gcgactgacc aagactttaa agaccaaaca aaagacggcc tcgtccttgt
     4321 tgacttctgg gcaacatggt gcggaccttg taaaatgatc gctcctgtat tggaagaact
     4381 cgaccaagac ttgaacggta aagcgaaaat cgttaaagtc gacgttgacg aaaaccaagc
     4441 aactgctgga gaattcggca tcatgtcaat cccgacactc gtcctcttca aagacggcga
     4501 aatcgtagac aaagttgttg gcttccaacc gaaagaacaa cttgccgaac tgattgaaaa
     4561 acacgcgtaa tgcattactt gaaaccggga accttcatag gaacccggtt ttttggtagg
     4621 tgagaaagat gaatgaacga atagaacata aactatcctt gctgccggac ttgcctggct
     4681 gctatctaat gaaagaccgc caaggcacaa tcatctacgt aggcaaggcg aaaatattga
     4741 aaaacagggt ccgcagttat ttcaccggat cccacgatgc aaaaacacag cgcctcgtag
     4801 gggaaatcga ggactttgag tatatcgtca catcatcaga tattgaggcg cttatcctcg
     4861 agctgaacct catcaaaca
//
LOCUS       DASULI010000428         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_830645, whole
            genome shotgun sequence.
ACCESSION   DASULI010000428 DASULI010000000
VERSION     DASULI010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830645"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="ribA"
                     /locus_tag="VFX34_09315"
     CDS             <1..1000
                     /gene="ribA"
                     /locus_tag="VFX34_09315"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012574662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5565149.1"
                     /translation="TPMTYQNTDVHGTAFTISIDHVNVHTGISAFERSETIMKMLEPD
                     TVPSDFRRPGHIFPLVAQPGGVLMRPGHTEAAVDLAKLAGSAPAGVICEIMNADGTMA
                     RGEQLAEIAKRFDLCILTIKDLIAYRKKKERLVERVVDIQLPTSFGEFKAYAYVETLT
                     GREHVALVKGSIEEMEAPLVRIHSECLTGDVFGSLRCDCGPQLHAALEQIEEAGAGIL
                     LYLRQEGRGIGLINKLKAYKLQEQGYDTVEANEQLGFADDLREYGIGAQMLEDLGVTR
                     IQLLTNNPRKISGLSDYGIDIVDRIQIELPVVEENRHYMQTKKMKLGHLLSHGGGI"
     gene            1000..1470
                     /gene="ribE"
                     /locus_tag="VFX34_09320"
     CDS             1000..1470
                     /gene="ribE"
                     /locus_tag="VFX34_09320"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5565150.1"
                     /translation="MKTTYEGHLIGTGLKIGIVVGRFNEFITSKLLGGAEDALRRHGV
                     SDEDVEVAWVPGAFEIPLIAKKMAASKKYDAVITLGTVIRGSTPHFDYVCNEVAKGVA
                     AINMSEGIPVIFGVLTTDSIEQAIERAGTKAGNKGWEAGAAAIEMANLMKQIEG"
     gene            1797..>2305
                     /locus_tag="VFX34_09325"
     CDS             1797..>2305
                     /locus_tag="VFX34_09325"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEX5565151.1"
                     /translation="MRNVTNEELEQLVEEARQYTYQGKVSEYIPALSLADKNHLSVAI
                     AYPDGSMFTAGNVHETFTLQSITKVIVLALALMDRGEEYVFQRVGMEPTGDPFNSIVK
                     LETHTPSKPFNPMINAGAIAVTSMIKGDSVDERINRILDLFRKLVGNSAVDYNRDVAF
                     SELATADLNR"
BASE COUNT          661 a          462 c          625 g          557 t
ORIGIN      
        1 gacgccaatg acgtaccaaa atacggacgt gcatggaaca gcttttacaa ttagcatcga
       61 ccatgtgaac gtccatacag ggatttctgc gtttgagcgt tcggagacaa ttatgaaaat
      121 gcttgagcca gatacagtac catccgattt tcgtagacct ggccacatct tcccgttagt
      181 tgcgcaacca ggtggagtat taatgcgccc tgggcatacg gaagcggctg ttgatttagc
      241 gaagcttgct ggttctgcac ctgctggcgt catctgtgaa attatgaatg cggatggaac
      301 aatggcaagg ggagagcaac tcgccgaaat cgcgaagcgt tttgatctat gcattcttac
      361 aataaaagac ttgattgcct accggaaaaa gaaagaacga cttgtggaac gagttgttga
      421 tatccaactg ccgacttcat tcggagaatt taaggcctac gcctacgtgg aaacattgac
      481 tgggcgcgag catgtggcat tggtgaaagg atcaattgaa gagatggaag cccctctcgt
      541 ccggattcat tcggaatgct taaccggcga cgtgtttggt tcattgcgat gtgattgtgg
      601 accacagctg cacgcagcac ttgagcagat tgaggaagcc ggagcgggca ttctgttgta
      661 tctccgccaa gaaggccgtg gaatcggact catcaataaa ttaaaagcct acaagttgca
      721 ggagcaggga tacgacacag tcgaagcgaa cgaacaatta gggtttgcgg acgatttgcg
      781 tgaatacgga atcggtgcac aaatgttaga ggatttgggc gtgacgcgaa ttcaactgct
      841 cacaaacaac ccgcgtaaga tttcgggttt gtccgactac ggcattgata ttgttgaccg
      901 gattcaaatc gagctgcctg ttgttgagga gaaccggcac tatatgcaaa cgaagaaaat
      961 gaagctcggg cacctattaa gccatggagg aggaatatga tgaaaacaac atacgaagga
     1021 catttaatag gaacaggttt aaaaattggg attgttgtag gaaggttcaa cgaatttatt
     1081 acaagcaagc ttcttggtgg agcggaagat gcgttgcgca gacatggcgt cagcgatgaa
     1141 gatgtggaag tcgcttgggt gccaggtgcg tttgagattc cgttgattgc aaaaaagatg
     1201 gcagcttcca agaagtatga tgcagtgatt acactaggaa cagtgatcag gggatcaact
     1261 ccgcactttg actatgtgtg caacgaagtg gcaaaagggg ttgccgctat taacatgtca
     1321 gaaggaattc cggtaatctt tggcgtcctc accacggatt ccattgaaca ggcaatcgaa
     1381 cgtgcaggaa cgaaagccgg gaataaagga tgggaagccg gagcggctgc gattgagatg
     1441 gcgaatttga tgaaacaaat cgaaggctaa taaaaatgac gacctgcgtt cttagaagga
     1501 acgaggtcgt catatttttt atttattgct agcgaatgtt cttgcaagtc cagcgattgt
     1561 accaacgatt acaaatgcga acaatgtaaa catgatgtat aggaaccaca tgagattttc
     1621 actccttttt ttcctcgaaa aatccaatgg gactcgatgt ctgctgccac cattatagca
     1681 tttaggcaag tgaccggcaa tggattcaac gagtcgtcgt tttcgaaatg tgacaaatac
     1741 gttataatgg gcggacgtat gttgaaagat tttcgcagtg gaaaggggtt tcggacatgc
     1801 gaaatgtgac aaatgaagaa ctggaacagc ttgttgagga agctagacaa tatacatacc
     1861 aaggaaaagt gtcggagtat attccggccc tgtcccttgc tgacaagaat cacctatctg
     1921 ttgcaattgc atacccggac ggctcaatgt ttacggcagg taatgtacac gagacattca
     1981 ctctgcaaag tattacaaag gtgattgtgc ttgcacttgc actgatggat cgaggggaag
     2041 agtacgtctt tcagcgggtc ggaatggagc cgacaggaga tccgtttaac tcgattgtga
     2101 agctggagac acacacacct tcgaagccgt ttaacccgat gatcaatgca ggcgcaattg
     2161 cggtcacgtc catgattaaa ggagattccg ttgatgagcg gatcaaccgg atcttggatc
     2221 tatttcggaa gctggtcggg aacagcgcgg tggattacaa ccgggacgtc gcgttttctg
     2281 aactcgcgac ggcggacttg aatcg
//
LOCUS       DASULI010000429         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1233313,
            whole genome shotgun sequence.
ACCESSION   DASULI010000429 DASULI010000000
VERSION     DASULI010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233313"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..613)
                     /locus_tag="VFX34_09330"
     CDS             complement(86..613)
                     /locus_tag="VFX34_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5565152.1"
                     /translation="MTTWKVDASHTSVGFSVKHMMVSKVKGSFAGVEGTIEGNPEDLT
                     NAKINFTIDANSINTNSEDRDNHLRSGDFFDTEKFPQLTFVSTDIVKKGDDEYEVTGE
                     LTIKDVTKKVTFEAEYEGTGTNPWGVQVAAFEVEGKISRKEFGLTWNQALEAGGVLVG
                     DDIKISIDLQANPAQ"
     gene            complement(840..3197)
                     /locus_tag="VFX34_09335"
     CDS             complement(840..3197)
                     /locus_tag="VFX34_09335"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5565153.1"
                     /translation="MGKWTKVLLSVFGGLLAVVIVGLIVINMYIGKSKPLIEGEVSVD
                     FLDADVEIMRDVNGVPHITASSDADLYRAQGFVQAQDRLFQMDLARRQASGRLAEVVG
                     AAAVDTDKTFRTFSLHNAAEASYDGYGDDAKKVLGWFAEGVNAFMQQAKDDGKLPYEF
                     KLLGYEPEPWTPIDSLTIGKYMAYDLGGNWDSLAVRHWALNNFPEEQARELFISYPDH
                     APSIIEANKKTPVEVAGRFNPDLVPHEFNGSNNWVVSGDKTASGKPLLADDPHLGLNT
                     PSIWYQMHLQSPEQNVSGVIFAGVPGIILGHNENIAWGVTNVGPDVQDLYIEIPNPED
                     PTQFRYDGKWEQAEVRDETISVKGEEDIPFEVIVTRHGPIMSEILYKSEDPGALFSMQ
                     WTALEPTKELEAIMRMNKAGDWETFEAALEDFHAPAQNFVFAAVDGTIAYKANGRIPI
                     RKQGDAQLPVPGDSSEYGWSGYVPYDELPKVVNPTEGFIATANNEVVDDSYPYHITKF
                     WAQPYRYMRIAEVLQGGDNFTAEDMMDLQMDQTNLYAREFLPDMIGSLKAVDSEGKYA
                     GFVRMLEEWDQVDSAEAGAPLVFHKVMKQLPKTMFSDEMPEDVYKLLPGKGQITDQML
                     RDAYSGNPGVWVSNYGGVDKWVFDAFESSIAEIEEQFGNDSAKWKWGDFHQLAFPHSL
                     AAASPIFAYFLNPEKQAIGGSNVTVQAAAFKDDGTVNHGASWRFVADLSDLSAAHHIV
                     GPGQSGHMKSKWFHNQAEDWANGIYHETVLDGEVKDGYTLKLKAE"
BASE COUNT          743 a          933 c          708 g          919 t
ORIGIN      
        1 cccttgtgca tttaatggag cccttggcga aataaaaacg gcatgcatga gttcacatct
       61 catgcgtgcc gttcgttttc aatacttatt gtgctgggtt tgcttgtaga tcgattgaga
      121 ttttaatgtc gtcgcctacg agcacaccgc ctgcttctag tgcttggttc catgtgaggc
      181 cgaactcttt acgagagatt ttgccttcga cttcgaaagc agcgacttga acgccccaag
      241 ggtttgttcc agtaccttcg tattctgctt caaatgtaac tttctttgtt acgtctttga
      301 ttgtcaattc acctgttact tcgtactcgt cgtcgccttt tttcacgatg tccgtagaga
      361 cgaatgtcag ttgagggaat ttttccgtat cgaagaaatc gccggaacga aggtgattgt
      421 cgcggtcctc gctattcgta ttgatagagt ttgcatcgat tgtgaaattg atttttgcat
      481 ttgtcagatc ttccgggttc ccctcaattg tcccttccac tcctgcaaag cttcctttga
      541 cttttgaaac catcatatgc ttgactgaaa aaccaacact cgtgtgtgat gcatccactt
      601 tccatgtcgt cattgtttta tcccctttca ttcttaccat tttttgtttg ataccctttt
      661 tccttatacc ctgattcctg aaagacattc cgctttgtga aaatatcttc attccaagat
      721 atattccata tgataaccta gatatgtctt gaagtcaaga tatgtatcca aggataaaag
      781 ttgcgcatct ccgattctca ttccaaaaag gaacgccctt tagcaagagc gtccccctat
      841 cattccgctt ttaatttcaa cgtgtaacca tctttcactt ctccgtcgag gacggtttcg
      901 tggtagatgc cgttcgccca atcctcggcc tgattgtgga accatttcga tttcatatgc
      961 ccgctttgac cgggtccgac gatgtgatgc gccgctgaca agtcggacag atccgcgacg
     1021 aaacgccatg acgcaccgtg attcacggtt ccatcatctt tgaaggcagc agcctgcaca
     1081 gttacgttcg agccgccgat cgcctgtttt tcagggttga ggaaataggc aaaaatcggt
     1141 gaagctgccg ctaatgaatg cgggaaagct aactggtgga aatctcccca cttccatttt
     1201 gccgaatcgt tcccgaactg ctcttcaatc tccgcaatgg acgactcgaa cgcatcaaaa
     1261 acccatttgt cgacgccccc ataattggac acccatacgc ccggattgcc gctgtatgca
     1321 tccctcagca tttggtcggt aatctgccct ttgcccggca acagtttgta tacatcttcg
     1381 ggcatttcat cgctgaacat cgtcttcggc aattgtttca ttaccttatg gaagacgagc
     1441 ggcgctcccg cttccgcgga atcaacctga tcccattcct caagcatcct gacaaacccg
     1501 gcatactttc cttcgctatc cacagccttc aacgagccga tcatgtccgg cagaaactcc
     1561 cgtgcatata aattcgtctg atccatctgc aagtccatca tgtcttccgc agtgaaattg
     1621 tccccgccct gcagcacttc ggcgattcgc atatagcgat aaggctgtgc ccagaatttc
     1681 gtaatatgat acgggtatga atcatcgaca acttcgttat tcgcggtcgc gatgaaacct
     1741 tcagtcgggt tgacgacctt tggcagttca tcataaggca catatccact ccagccatat
     1801 tcagacgaat cacctggcac cggcagctgg gcgtcccctt gtttgcgaat cggaattctt
     1861 ccgttcgctt tataggcgat tgtcccgtcc accgcagcaa acacgaagtt ctgggcaggc
     1921 gcatggaaat cttccaatgc ggcctcgaac gtctcccagt ctcccgcttt gttcatccgc
     1981 atgatcgctt cgagctcttt cgtcggttcg agtgccgtcc attgcatgga gaacaacgca
     2041 cccggatcct cggatttata aaggatctcc gacatgatcg gcccgtgcct cgtgacaata
     2101 acttcaaaag ggatgtcttc ctcccctttt accgaaatcg tctcgtcccg cacttcggcc
     2161 tgctcccatt tcccgtcgta gcggaattgg gttgggtctt ccggattcgg gatttcaata
     2221 tacaaatcct gcacgtcagg cccgacattc gtgacgcccc atgcaatatt ctcgttatgg
     2281 ccgaggatga tgcccggaac gcctgcaaaa atgacaccac tgacgttctg ctcaggcgac
     2341 tgcaaatgca tttgatacca aatcgatggc gtgttcaatc ctagatgggg atcatccgcg
     2401 agaagcggct ttccggaagc cgtcttatca ccggaaacga cccagttatt gctgccattg
     2461 aattcatgag gcaccaaatc cggattgaag cggcccgcca cttcaacagg cgttttttta
     2521 ttcgcttcga tgatcgaagg tgcatgatcc ggatagctga tgaataattc acgtgcctgc
     2581 tcttccggga aattgttaag tgcccaatgt ctgacagcga ggctgtccca gttgccccct
     2641 aagtcgtagg ccatgtattt cccgattgtg agcgagtcaa tcggcgtcca cggctcaggc
     2701 tcatagccaa ggagcttgaa ttcataaggc aatttcccgt cgtccttcgc ctgctgcatg
     2761 aaagcattga cgccttccgc gaaccagcca agtaccttct tggcgtcgtc gccatatccg
     2821 tcgtaggaag cttctgccgc attgtgcaaa ctgaatgtcc ggaatgtctt atccgtatcg
     2881 acagctgcgg caccgaccac ttcagcgagc cgcccgctcg cctgcctcct cgccaaatcc
     2941 atttggaata aacgatcctg tgcctgcaca aagccttggg cccgatacaa gtcggcatcg
     3001 ctactggcgg taatatgcgg cacgccgttc acatcccgca tgatttccac gtcggcatcc
     3061 aagaagtcca cgcttacctc cccttcgata agcggcttcg attttcctat gtacatattg
     3121 atgacaatca gcccgacgat gacaacggca agcaatccgc caaagacgga taacaatacc
     3181 tttgtccact tccccatttt cctttttctc aacgtgcatc ccccctgcta tacaattcta
     3241 tgaatggtca ttcatcccct ttatttccaa ctggaatttt tcaacatccg gaaacccggt
     3301 tat
//
LOCUS       DASULI010000430         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1842559,
            whole genome shotgun sequence.
ACCESSION   DASULI010000430 DASULI010000000
VERSION     DASULI010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842559"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1429
                     /gene="fliI"
                     /locus_tag="VFX34_09340"
     CDS             98..1429
                     /gene="fliI"
                     /locus_tag="VFX34_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX5565154.1"
                     /translation="MKKAKELLDVIPKMNSFPKFGRVVRVVGLMIESQGPESSVGDVC
                     RIHLNNSGKRDSVILAEVVGFREEIVILMPYSNITDISSGCLVECTGKPLEVKVGMNL
                     IGKVLDSMGNPIDDSPLPKGLSTVRTEQNPPNVMTRLPISEKLAVGVKAIDGMLTVGS
                     GQRVGIFAGSGVGKSTLLGMIARNTTADLNVIALIGERGREVREFIERDLGPEGLSKT
                     IVVAATSDQPALMRIKGAFTATAIAEYFRDKGMNVMLMMDSVTRVAMAQREIGLAVGE
                     PPATRGYTPSVFSILPKLLERTGTNHVGAITAFYTVLVDGDDMNEPIADAVRGILDGH
                     IVLDRTLANKGQYPAINVLKSVSRLMNHIASPEHVKAAEKLRELYYTYDKSEDLINIG
                     AYKRGTSREIDEAIEYEPHITNFLKQSYKENVMMEDSIEELISLAPGGGTR"
     gene            1426..1878
                     /gene="fliJ"
                     /locus_tag="VFX34_09345"
     CDS             1426..1878
                     /gene="fliJ"
                     /locus_tag="VFX34_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX5565155.1"
                     /translation="MKPYEYRFEKVLTYREQEKTETEIEFKKAVESFETVATELYDLL
                     KRKEDIIADHQEKMKKGFSINEIHHYARFLDSLEKRIESLQQSVLKARSKMNWFEEKL
                     LEKTIEVKKFEKMKERDKEHYRTEMEQAEASLLDELSTLKFHRRETGW"
BASE COUNT          618 a          409 c          546 g          453 t
ORIGIN      
        1 ttgaaacaaa ccagggccgg gtagttgtca gtgtggatga acaattgaac gagctgaaag
       61 agcagctcgt tgaaatgttg gaaagtggtg actgacgatg aagaaagcga aagagttgtt
      121 ggacgtcatt ccgaaaatga attcctttcc aaaattcggt cgcgttgtcc gggtcgtcgg
      181 cctaatgatc gaatctcaag gccctgaaag ctctgtcggc gatgtatgcc gcatccatct
      241 gaataacagc ggcaaaaggg actcggtcat tttggcggag gttgttggat tcagggagga
      301 aattgtcatc ctgatgcctt actcgaacat caccgatata tcgagcggat gtctggttga
      361 atgcaccgga aagccacttg aggtgaaggt cggtatgaac ctgatcggaa aagtgcttga
      421 ctcgatggga aatccgatcg atgacagtcc gttgcctaaa gggttatcga cagtaaggac
      481 agagcagaac cctccaaatg tcatgacgag attgccgatc agtgaaaaac ttgcagtcgg
      541 tgtgaaggcg attgacggga tgttgacggt tggaagtggc caaagagttg ggatcttcgc
      601 aggttccggc gttgggaaaa gtacattgct tggcatgatt gcaagaaata cgactgcaga
      661 tttgaacgtc atcgcactta tcggagagcg cggccgcgaa gtcagggaat tcatagaacg
      721 ggatttaggg ccggaaggat taagcaagac aatcgtcgtt gccgcaacat ccgaccaacc
      781 ggcattgatg aggataaaag gtgcatttac ggctacagcg atcgctgaat acttccgcga
      841 taaagggatg aacgtcatgc tcatgatgga ttccgtcacg cgtgtcgcaa tggcgcaaag
      901 ggaaatcgga ctcgctgtcg gagaaccacc cgcaacacgc ggttacacgc cgtccgtatt
      961 ctcgatatta ccgaaactcc ttgagcggac cgggacgaat cacgtcggtg cgattacggc
     1021 attttacact gtgctggtcg atggcgatga catgaatgaa ccgattgcgg atgcggtccg
     1081 gggaattctt gacgggcata tcgtattgga caggacactt gcaaacaaag ggcaatatcc
     1141 ggcaataaac gtcctgaaaa gtgtaagccg tcttatgaat catatcgcca gtcccgaaca
     1201 tgtgaaggcc gctgaaaaac tccgggaatt gtattacaca tatgacaagt cggaagatct
     1261 catcaatatc ggcgcgtata aacgcggcac atcaagggaa atcgacgaag cgatcgaata
     1321 tgaacctcac attacgaact tcttgaagca gagctataaa gagaatgtga tgatggaaga
     1381 ttccattgaa gagctgatat cacttgcacc aggaggaggc acacgatgaa accttatgaa
     1441 taccgttttg aaaaggtgct aacttaccgg gaacaggaga aaacggagac cgaaatagag
     1501 tttaagaaag cagtcgagtc tttcgaaacg gtcgcaaccg aattatatga tctgttgaaa
     1561 cggaaggaag acatcatcgc cgatcaccag gagaaaatga aaaagggttt ttccatcaat
     1621 gaaatccatc actatgcccg atttttggat agtctcgaaa aacggattga aagcttgcag
     1681 caatccgtcc tgaaagcccg ttccaaaatg aattggttcg aagagaaatt gcttgagaag
     1741 acgattgaag tgaagaagtt cgaaaagatg aaggaaagag ataaagaaca ttatcgtact
     1801 gaaatggaac aggcggaagc aagtttgttg gatgaactat caacattgaa gttccataga
     1861 agagaaacag ggtggtaatt tggcaaggaa ctcaaaactg caaactgacg agaaaatcgt
     1921 tgaaaagaag acaggtaccc gcgtcttcca ggaagtgttg ctttggaccc tcattccact
     1981 tctgttcaca acgatggtgc ttttgattat cgcctatgtc gccgat
//
LOCUS       DASULI010000431         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_636745, whole
            genome shotgun sequence.
ACCESSION   DASULI010000431 DASULI010000000
VERSION     DASULI010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636745"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFX34_09350"
     CDS             <1..1089
                     /locus_tag="VFX34_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5565156.1"
                     /translation="GRLAHSFDHMKNELGSMIKHVNAAADNVVNSSSELASSAEQMSE
                     VVNQSTVATQEVSASSESLSVAANQNLTALEQITAGIQDIADSTSKVAEETLEVSEAA
                     EQGSQLITDSIEGIHTINESVQASMKITETMHIRSSEIEKIIGMITDISSQINLLALN
                     AAIEAARAGEAGKGFSVVASEVRNLAEQSAASANQIRALITEMQNGSRQSVEAMAKVY
                     ADVERETVIVNDAGKTFGSIMSKIANISDNVQSVSATVQEISAGSEQILASTHDTVQS
                     LEVSSGHTQNIAASMEEQLASMEEMVATTDTLHDLATELKMEISKFRVDEEVQVDEVL
                     NEEPSEEIQDDLEIQDEEETQEDERSVQ"
     gene            complement(1216..2247)
                     /locus_tag="VFX34_09355"
     CDS             complement(1216..2247)
                     /locus_tag="VFX34_09355"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX5565157.1"
                     /translation="MAKKELLKVEGLKQYFPIKGGMMGRTINHVKAVDDISFTIYEGE
                     TVSIVGESGCGKSTTGRAILRLEEPTEGVVTFNGHELTSLSKREMRKQRKDLQIIFQD
                     PYASINPRQTVADVLDEAMVIQNVLPAKERRARIIELLETVGLNEYQADRYPHEFSGG
                     QRQRIGIARALSVNPKLIICDEAVSALDVSIQAQVLNLLKDLQREFKLTYLFISHDLG
                     VVRHISDRVIVMYLGKIVEIGDKYSIFENPKHPYTQALLSAIPVPDPTREKKHIVLTG
                     DVPSPIDPPEGCRFHTRCPFAQEKCKVDVPVLRTHESMITGHKAACHFVEEIENGELQ
                     VKGFEEVVF"
     gene            complement(2192..3244)
                     /locus_tag="VFX34_09360"
     CDS             complement(2192..3244)
                     /locus_tag="VFX34_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018933557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5565158.1"
                     /translation="MVTDLLEVRDLRTSFYTDDGEVKAVDGVSFTLPHGKTLGIVGES
                     GSGKSITALSILRLLANNGKIIGGEVRFKGEDLLSYSDKQMRDIRGNAISMIFQEPMT
                     SLNPVFTVGQQISESLMKHQDMSKKAAHLKSIELLKLVGIPSPDKRVRQYPYELSGGM
                     RQRVMIAMALACNPEILIADEPTTALDVTIQAQILRLIRDLQKRLGMSVIMITHDLGV
                     VAETCEYVAVMYAGQVVEYSDVVTLFQKPKHPYTIGLMNSLPRHDLELEKLESISGNV
                     PNPKEMPTGCRFAPRCPAATDLCRNVMPDLLKDDEGNDVRCWIYSEKWEGESEVIVYG
                     EKRVAQSRGAETILSD"
     gene            complement(3263..>3847)
                     /locus_tag="VFX34_09365"
     CDS             complement(3263..>3847)
                     /locus_tag="VFX34_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006207784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5565159.1"
                     /translation="SAVRHYTWSDISIMIVALFGLSMPNFWFGLMLIQWFAIGPDWLP
                     DFMKMRPSGWGDDWRQLLLPVITLGTGGAAIIARMTRSSMLEVIGQDYIRTARAKGMG
                     ERVVIYRHALKNAMIPVITVVGLEFGGFLGGAVLTESVFAINGMGRLTIDAIRTRDFP
                     IVQGTVLVISFLFVFVNLLVDISYRVLNKRIDLN"
BASE COUNT          981 a         1073 c          912 g          881 t
ORIGIN      
        1 gggcggcttg cccatagttt cgatcatatg aaaaatgaac ttggcagcat gatcaagcat
       61 gtgaatgcgg cggcggacaa tgtcgtaaac tcgtccagtg aattggcatc cagcgccgag
      121 cagatgagtg aagtcgtgaa ccagtcgacg gtcgcgacgc aggaagtttc ggcgtcaagc
      181 gaatcgcttt ccgtagccgc caatcaaaac ctgacggcac ttgagcagat taccgcgggc
      241 atccaggaca tcgccgactc cacttcgaaa gtggcggaag agacattgga agtatccgaa
      301 gcggcggagc aaggaagcca gctgatcaca gactcgatcg agggaatcca tacgatcaat
      361 gaatccgtgc aggcatcgat gaaaattacg gaaacgatgc atatccgttc atccgagatc
      421 gagaaaatca tcggcatgat caccgacatt tccagtcaaa tcaaccttct cgcattaaac
      481 gccgccatcg aagcggctcg cgcgggtgag gcaggaaaag ggttcagcgt cgttgcaagc
      541 gaagtgcgca acttggcgga gcaatctgca gcttccgcga atcagatccg tgcgctcatc
      601 actgaaatgc aaaacggttc ccgtcaatcc gttgaggcga tggcgaaagt atacgccgat
      661 gttgagcggg aaacggtcat tgtcaacgat gccggaaaaa cgttcggcag tatcatgtcg
      721 aaaatcgcga acatctcgga taatgtccaa tccgtttccg caacggtcca ggaaatttcg
      781 gccggctccg aacagatttt ggcgtcgacc cacgacactg tccagtcgct tgaagtatct
      841 tccggccaca cgcaaaatat cgcggcttcc atggaagagc agcttgcctc aatggaggaa
      901 atggttgcga caacagatac cttgcatgac ttggcgactg aattgaaaat ggaaatcagt
      961 aaattcaggg tggatgaaga agttcaagtc gatgaagtcc tgaatgaaga gccaagtgaa
     1021 gaaattcagg atgacctaga aattcaggat gaagaagaaa cgcaagaaga tgaaagatcc
     1081 gttcaataaa ccaacaccgc ctgtcaattg atgggcggtg tttttgatta gctgaaatga
     1141 acccagcatc catccccaaa caaaaaacca cctgccaaaa gaataaactt ttgaccaggt
     1201 ggtatattca atcttctaaa acaccacttc ctcaaacccc ttcacttgca gctcgccgtt
     1261 ttcaatctcc tccacgaaat gacaggcagc tttgtgtcct gtaatcattg attcatgcgt
     1321 gcgcaataca ggcacatcga ctttgcattt ctcctgtgcg aacggacagc gtgtatggaa
     1381 gcggcaacct tccggcggat cgatcgggga cgggacatcc cccgtcagga cgatatgctt
     1441 tttctcccgt gtcgggtccg gcaccggaat ggcggaaagc aaggcttgcg tgtacggatg
     1501 cttcggattt tcgaaaatcg aatatttgtc gccgatttcg acgatcttcc ctaaatacat
     1561 gacgatgacc cggtccgaaa tatgacggac gacgccaaga tcatgcgata tgaataaata
     1621 cgtcaatttg aattccctct gcagatcctt cagcaggttc agcacttgcg cctgaatcga
     1681 gacgtccaac gcagaaacgg cttcatcgca gatgatcaat ttcgggttga ccgataatgc
     1741 ccgggcaatc ccgatgcgct gccgctgtcc gcctgaaaat tcatgcgggt accgatccgc
     1801 ttggtattca ttcaacccga ccgtttccaa aagctcaatg atgcgcgccc tgcgctcctt
     1861 tgcaggcaac acgttctgga tcaccatcgc ttcatcgagg acatccgcca cggtttggcg
     1921 cgggttgatg gacgcgtatg ggtcttggaa aatgatctgc aaatctttcc gctgcttcct
     1981 catctcccgt ttcgacagtg atgtcagttc atgcccattg aatgtgacga cgccttccgt
     2041 cggttcttcc aatcggagga tggcgcggcc cgtcgtcgat ttcccgcagc cggactcccc
     2101 gacgatgctc accgtctccc cttcgtaaat cgtgaaactg atatcatcga ccgctttcac
     2161 atggttgatt gtgcggccca tcatcccgcc tttaatcgga aagtattgtt tcagcccctc
     2221 gactttgagc aactcttttt tcgccataca caatcacctc cgattcacct tcccattttt
     2281 ccgaataaat ccagcaacgc acatcgttcc cttcatcatc cttcaacaaa tccggcatga
     2341 cgttcctgca aagatcggtc gccgccgggc atctcggcgc aaacctgcag cctgtaggca
     2401 tctccttcgg attcggtaca ttcccgctga tcgactccaa tttttcaagc tccaggtcgt
     2461 gccttggcaa tgaattcatc agtccgatcg tatacggatg cttcggcttt tgaaaaagcg
     2521 tcacgacatc cgagtattcg acaacctgcc cggcatacat gacggcgaca tattcgcatg
     2581 tttccgccac gactccgagg tcgtgcgtaa tcatgatgac cgacatgcca agccgcttct
     2641 gcaaatcgcg gatgagcctg agaatctgcg cctggattgt cacgtcgagc gctgtcgtcg
     2701 gttcatctgc aatcagaatt tcaggattgc atgcaagcgc cattgcaatc atgacccgct
     2761 gccgcatgcc ccccgacaat tcatacggat attgcctcac gcgtttatcc ggtgaaggga
     2821 tcccaacaag tttcagtagc tcgatagatt tcagatgcgc cgctttcttt gacatgtcct
     2881 ggtgtttcat tagcgactcg ctaatttgct ggccgactgt aaagaccggg ttcaacgatg
     2941 tcatcggctc ctggaagatc atcgaaatcg cgtttccgcg aatatcccgc atttgcttgt
     3001 ccgaatagga cagaagatcc tcccctttga atcggacttc cccaccgatg attttgccgt
     3061 tgttcgcaag tagcctcaag attgaaagcg ctgttatgct cttgcctgac cccgattcac
     3121 cgactatccc gagcgtcttc ccatgcggaa gcgtgaaact gacgccgtca accgccttca
     3181 cttccccgtc atctgtataa aaggacgtgc ggagatcccg cacttccaat agatccgtca
     3241 ccatttaaga cacccccctg tatcagttca aatcaatccg cttattcaag acgcggtacg
     3301 atatgtcgac gagcagattc acaaatacga acaggaaaga aatgacgagg acggtccctt
     3361 gtacaatcgg gaagtcccgc gtccggatcg cgtcaattgt cagacgcccc atgccgttga
     3421 ttgcaaaaac tgattccgtc aatacggctc cgccaagaaa accaccgaac tcaagtccaa
     3481 ctaccgtaat gaccggaatc attgcgtttt tcagcgcgtg gcggtaaatg acaacgcgct
     3541 cccccatccc tttcgccctc gccgtccgga tgtagtcttg cccgatcact tcaagcatcg
     3601 atgaacgggt catccgcgca atgatcgccg ctcctcctgt tcctagcgta atgacaggca
     3661 gaagcaactg acgccaatcg tccccccagc cggatggacg catcttcata aagtccggca
     3721 accaatccgg accaattgca aaccattgga tcaacatgag accgaaccag aaattcggca
     3781 tggacaaacc gaataaggcg acaatcataa tggatatgtc agaccatgtg taatggcgga
     3841 cagccga
//
LOCUS       DASULI010000432         3980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_228044, whole
            genome shotgun sequence.
ACCESSION   DASULI010000432 DASULI010000000
VERSION     DASULI010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228044"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFX34_09370"
     CDS             complement(<1..215)
                     /locus_tag="VFX34_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565160.1"
                     /translation="MKLSRQVIWFSLAMMAVMVVIGVAMFMERENVSAFDPIESINSA
                     ADLKAYFERAKDMQGVSVKYDMVSEEKA"
     gene            357..1670
                     /locus_tag="VFX34_09375"
     CDS             357..1670
                     /locus_tag="VFX34_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEX5565161.1"
                     /translation="MKTLTRWSNTIMERYLPDPYIFVAILTLLVFLLGIGLTDSGPLE
                     MTVHWGNGFWGLLAFTMQMVIVLVAGHVLASSPFFKKLLSTLAGLAKSPGTAILLVTV
                     VSLIACWINWGFGLVIGALFAKEIAKKVTTVDYRLLIASAYSGFIIWHGGLGGSIPLS
                     IATEDHPYANMMGVVPTSETIFSTYNLFIIAVIFITLPLLNRFMMPKPEDTVTVDPEL
                     LEDEIEVEEAEEKTPAARLENSVILSMLIGALGVVYLVQHFVTKGFDLNLNIVNLIFF
                     ILGIIFHGTPKRFLAAVANAVKTSGGIIIQFPFYAGIMGMMVDSELAKVMSQWFVSIS
                     TEATFPLFTFYAAGLVNFFVPSGGGQWAVQAPVMLDAAQTLGVSYSKTAMAIAWGDAW
                     TNMIQPFWALPALAIAGLRAKDIMGYCVFVLLLSGIVVSIGLYFF"
     gene            complement(1714..2316)
                     /locus_tag="VFX34_09380"
     CDS             complement(1714..2316)
                     /locus_tag="VFX34_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEX5565162.1"
                     /translation="MKTLLLTGFEPFLNYPVNPTARISEELHGLEIGGYRIHSEVLPV
                     DFSVSGDRILSHVEAVKPDAVLSLGLSGGRFKITPERIAINVNDGAKDNSGHAPVDEP
                     IIEGGADGYFTNMPIRKMVDALKGAGLPAEISNTAGAYLCNHVMYRVLHFVNEQRPDM
                     QAGFIHIPASHELAVEHGRVPSWSHEDLKCAIVTCIEQLD"
     gene            2467..3348
                     /locus_tag="VFX34_09385"
     CDS             2467..3348
                     /locus_tag="VFX34_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012370087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5565163.1"
                     /translation="MPKDKYEKKDEQVTPQTQDRQPGIEAEMDPLPLYDDENYRGSGK
                     LKGKNALITGGDSGIGRAVAVAFAKEGANVAIAYLDEAEDVDANKTVELIEQYGGKAK
                     KICIDISEEENCEKLIDDVIQEFGSLNILVNNAGKQFPQDDISQISSDQLRETFDTNF
                     FGLFYLSKAALAHMKEGDCIINTSSVTAYNGSPGLIDYSATKGAITTFTRSLALNLAA
                     KGIRVNAVAPGPIWTPLIPATFDAKKVEQHGADTPVKRRGQPAENAPAYVFLASSDSS
                     YMTGQTIHVDGGDYVGS"
     gene            complement(3567..>3980)
                     /locus_tag="VFX34_09390"
     CDS             complement(3567..>3980)
                     /locus_tag="VFX34_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEX5565164.1"
                     /translation="VGIIKGALGMETVNLPLFILTAIITSYTFIAIVQMLVSLFGDPG
                     RFMAIIILILQLTTSAGTFPLELIPEPLQVFNTLLPMTYSVQAFKASISTGDLAHLWA
                     SNGVLLGFMLACLAITFGYFALIFKKRYSRDTVEA"
BASE COUNT         1019 a          987 c          983 g          991 t
ORIGIN      
        1 gctttctcct ccgaaaccat gtcatatttc accgaaacac cctgcatatc ttttgccctt
       61 tcaaaatagg ctttcaaatc agctgctgaa ttgatagatt cgatgggatc aaaggcagaa
      121 acattctccc tctccataaa cattgcgaca cctataacaa ccatgaccgc catcatagcc
      181 aaactgaacc aaattacctg tctactcaat ttcatcacct ttcctcatct gcttctttac
      241 aaagtagact tgctggaaac aattacgttt cacatttcgg aaaaaacatt tttgcggatg
      301 aattcgatta tggtagtcta gtagggatgg gtaaaaaata tagggggtaa aagggaatga
      361 aaacacttac aagatggtcg aacaccatca tggaaaggta cttgccggat ccatacatat
      421 tcgttgccat tctgacattg ctcgtctttt tgcttggcat cggcttgaca gattcgggtc
      481 cgctcgagat gactgtccac tggggtaatg gtttctgggg gcttctcgcc tttacgatgc
      541 agatggtcat cgtgttagtc gcgggacatg tattggcaag cagtccattt ttcaagaaat
      601 tattaagtac attggcagga ttggcgaagt cacctggtac agcgattctg cttgtgacag
      661 tcgtatcact tatcgcttgt tggatcaact ggggattcgg tctcgtcatc ggggcgttat
      721 tcgctaagga aattgccaaa aaagtgacga ctgtcgatta ccggctgtta attgcaagcg
      781 cttactcagg tttcatcatt tggcatggag ggcttggcgg atcgattcct ttgtccatcg
      841 caacggagga tcatccatac gctaacatga tgggtgtcgt cccaacgtcc gaaacgattt
      901 tttcaacgta taatctattc attatcgcag tcatattcat aactttaccg ttgttgaacc
      961 gtttcatgat gccaaaaccg gaggatacgg tcaccgtcga tccggaactg ttggaagatg
     1021 aaatcgaagt cgaagaagca gaggaaaaaa cgcctgctgc acggcttgaa aacagtgtga
     1081 tcctatccat gctgatcggt gcattgggtg tcgtctatct cgtgcagcac tttgtcacga
     1141 aaggcttcga tttgaacctg aatatcgtca accttatttt cttcatcctc ggcatcattt
     1201 tccacggcac gccgaagcgt ttccttgcgg cggttgccaa tgcggtgaaa acgtcaggcg
     1261 gaatcatcat ccagttccca ttctacgcag ggatcatggg catgatggta gattcagaac
     1321 tagcgaaagt catgtcccaa tggttcgtca gcatttcaac cgaagcgact ttcccgcttt
     1381 tcacatttta tgcggcggga ctcgttaact tcttcgtacc ttcaggcggc ggacaatggg
     1441 ctgtacaagc gccggtcatg ctggatgcgg ctcaaacgct cggcgtcagc tactcgaaaa
     1501 ccgcgatggc catcgcatgg ggcgacgcgt ggacaaatat gatccagcca ttctgggcat
     1561 taccggcact cgcaatcgca ggattgcgcg cgaaggacat catgggctat tgcgtgttcg
     1621 tcttgttatt gagcggtatt gtcgtgagta tcgggctata tttcttttaa tgtatcaaaa
     1681 agctgggaga gccattctcc cagctttttt cattcaatcc aactgttcaa tgcatgtcac
     1741 aatcgcgcat ttcaagtctt catgggacca gcttggcacc cggccgtgct cgacggcgag
     1801 ttcgtgggaa gccgggatgt ggatgaagcc cgcttgcatg tcggggcgct gttcattcac
     1861 gaaatgaagg acgcggtaca tgacgtggtt gcataaatag gcgccggccg tattcgaaat
     1921 ttcggcgggc agtccggcac ccttcaacgc atcgaccatt ttccggatcg gcatattcgt
     1981 gaaatagccg tccgctcccc cttcaattat cggctcgtcg acaggcgcat ggccgctatt
     2041 gtccttggca ccatcattta cattgatggc gatccgttcg ggtgtaatct tgaatcggcc
     2101 gccggataag ccgagcgata gcaccgcatc cggtttgacc gcttcaacat gcgaaaggat
     2161 acggtctcct gaaacgctga aatcaacggg cagcacttca ctatggatcc ggtatccccc
     2221 gatttccaac ccatgcaatt cttctgaaat ccgcgcagtc ggattgacgg ggtaattcaa
     2281 aaacggctca aagccggtta atagcaatgt tttcatacta ttctcccctt tctctccata
     2341 ttataacgga tttacaatgg tctggatggt tttgttccgg ctaacttcga ttttcatccg
     2401 ccacgtttat gagaccccat acagggaata tgccaactag atgatttttt gaaggaggaa
     2461 cagtgcatgc ccaaagataa atatgaaaaa aaagatgaac aagtgacgcc gcagacacag
     2521 gatcggcagc caggtatcga agcggaaatg gatccgctgc cactgtatga cgatgaaaac
     2581 tatagaggat ccggcaaatt gaaaggaaaa aacgctctga tcaccggcgg agacagcggc
     2641 atcggtcgcg cggttgcagt cgcgtttgcg aaagaaggtg cgaacgtcgc gattgcctac
     2701 ttggatgagg cggaagatgt cgatgcgaat aaaacggtcg agctcatcga acaatacggc
     2761 ggaaaagcga agaaaatctg catcgacatt agcgaagagg aaaattgcga gaagctgatc
     2821 gacgatgtca tccaggaatt cggcagcttg aacatccttg tgaataatgc cggaaagcag
     2881 tttccgcagg acgacatctc ccaaatttcg agcgatcagc tccgtgaaac gttcgacacg
     2941 aactttttcg gcctatttta tttatcgaag gcggcgctcg cccatatgaa agaaggcgat
     3001 tgcatcatca acacctcgtc cgtcacggcc tataacggct cgccgggatt gatcgactac
     3061 tcggcaacga aaggcgcgat tacgaccttc acccgctccc tcgccttgaa cttggctgcg
     3121 aaagggatcc gcgtcaatgc agtggcacca ggtccgatct ggacgccgct cattccggcg
     3181 acgttcgacg cgaaaaaagt ggagcagcat ggggcggaca cgccagttaa gcgccgtgga
     3241 cagccggcgg agaacgcacc cgcttatgtc ttcctcgctt ctagcgattc cagctatatg
     3301 acggggcaga caatccacgt ggatggcggg gattatgtag gttcttgatt gtttcatggg
     3361 gactgtgccg cctgcttcgg acgcctaatg atgcgatttg agcgcgggac ttgaatcttg
     3421 agcgcggaag tcaattcttg ggcgcagaac tcgaatcttg agcgcggaat tcaattcttg
     3481 agcgcagaac ccactctttg agcgcttttg gaaccgccca cactaaaaaa gcgatttgca
     3541 gatcaccatc cgcaaatcgc tttttcttat gcttccacag tatcacgtga ataccgcttt
     3601 ttgaaaatca atgcaaaata gccaaacgtt atcgccaagc atgcgagcat gaagccgagc
     3661 agcacgccgt tgcttgccca aagatgcgcg agatccccag tagagatcga cgccttgaat
     3721 gcttggacgg aatacgtcat cggcaataat gtgttgaaca cttgaagagg ttcaggaatt
     3781 aattccaacg ggaacgtccc tgcactcgtc gtcaattgca aaatcaagat gatgatcgcc
     3841 ataaaacgtc ccggatcacc gaatagcgac accagcatct gcacaattgc gatgaacgtg
     3901 tagctcgtaa tgatcgccgt caaaatgaac aacggcaagt tgaccgtctc cattccaagc
     3961 gctcctttga tgatgccgac
//
LOCUS       DASULI010000433         7800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_168488, whole
            genome shotgun sequence.
ACCESSION   DASULI010000433 DASULI010000000
VERSION     DASULI010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168488"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1403)
                     /locus_tag="VFX34_09395"
     CDS             complement(171..1403)
                     /locus_tag="VFX34_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEX5565165.1"
                     /translation="MPKSTMKNLFSMSLRSKQIAIVAFTFALFVSIISLVLYEVSKKS
                     VTLHVNDEQIEIKTSAHTVGQLLSSEDIEVAEHDLVTPSVNTPIEEGISIWWEQAKQV
                     EIQTGNDTEKIWTTDKNVGDILAKAGIELTEHDEVTPNLNENLGENLHINIQKAFQLT
                     LIDGGNEKQMWSTSTTVADFLRKENIQLNELDKLNQKADVFVKPGSIIEVVRVEKVTD
                     VVEEPTNFAVVTKSDAKLLKGREKVVQEGKKGSVERKFEIVKENGKEVSRTLLSERVI
                     EEPQKKIVAVGSKVVVASASIKKTNVAVSRGSSAAPSGGKEFYVTATAYTAHCNGCTG
                     KTATGIDLRANPNLKVIAVDPSVIPLGSKVWVEGYGYAIAGDTGGAIKGKKIDLHVPT
                     KAAAYNFGRRTVKIKVID"
     gene            complement(1535..2305)
                     /locus_tag="VFX34_09400"
     CDS             complement(1535..2305)
                     /locus_tag="VFX34_09400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5565166.1"
                     /translation="MYIDTHVHLNADQYEEDVKEVIQRALEAGVERMVVVGFDRVTIE
                     KAMELAEHYPFIYAVIGWHPVDAVDCTEEDLKWIESLAAHPKVVGIGETGLDYYWDKS
                     PKDIQQELFRKQIRLAQKVNLPVIIHNRDATGDVVRILKEEEAGITGGIMHCYGGSVE
                     TAKECIDMNFLISLGGPVTFKNAKMPKQVAMEIPLDHLLIETDAPYLAPHPHRGKRNE
                     PALVTLVAEEIARLKEIPVEEVAKRTTENALKIFRIPK"
     gene            complement(2426..4387)
                     /gene="metG"
                     /locus_tag="VFX34_09405"
     CDS             complement(2426..4387)
                     /gene="metG"
                     /locus_tag="VFX34_09405"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5565167.1"
                     /translation="MAEQQKTFYITTPIYYPSGKFHIGTAYTTVASDAMARYKRLRGY
                     DVRFLTGMDEHGQKIQEKAEEEGLPPQQYVDRIADIAIDLWKLMDISYDDLIRTTEER
                     HKVAVEKIFQKFLDNGDIYKGEYEGWKCVPCESYFTEAQLEDGNCPDCHRPVERVSEE
                     SYFFNMKKYADRLLQYYEDNPTFIEPESRKNEMINNFIKPGLEDLSVSRMSFDWGIKV
                     PGDPKHVIYVWVDALTNYITALGYGSDDETLFNKYWPADVHVVGKDIVRFHTIYWPIF
                     LMALDLPLPKKVFAHGFIMMKDGKMSKSKGNVVYPEMLVERYGLDATRYFLLRELPFG
                     QDGTFSPESFVERTNYDLANDLGNLLNRTVSMINKYFDGVIPTEGLEPTEFDSVLRDF
                     TTNAIEKYEAAMEKMQFSVVLSELWTIISRTNKYIDETSPWVLAKEEADKGKLASVMA
                     HLAASLRQIAVLLQPFMTKAPIQIIEQLGLDDKMLAWETLSDFNAIPEGMKVVEKGIP
                     IFPRLDPEVEIVYIRDQMAITAPQEKQEQEPVKAEEEAEEITIDDFMKVDLRVATVTA
                     CEKIPKADKLLKLQVDLGYEQRQVVSGIAEHYEPEALIGEKVIVVANLKPVKLRGELS
                     QGMILAGSSNGVLKLATVDATLENGAQVK"
     gene            4866..5141
                     /locus_tag="VFX34_09410"
     CDS             4866..5141
                     /locus_tag="VFX34_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX5565168.1"
                     /translation="MKSTGIVRKVDELGRVVIPIELRRTLGIEQKDALEIYVDDDKII
                     LKKYMPNMTCSITGEVSDNNLQLIDGKLILSPDGAKQLIKEIEEKIK"
     gene            complement(5197..6075)
                     /gene="rsmI"
                     /locus_tag="VFX34_09415"
     CDS             complement(5197..6075)
                     /gene="rsmI"
                     /locus_tag="VFX34_09415"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5565169.1"
                     /translation="MKSQKSAETGVAKLFLVGTPIGNLEDMTFRAIRILQEADMIAAE
                     DTRNTIRLCNHFEIKTPLMSYHEHNLEKGGEKILSLLAEGKTVALVSDAGMPCISDPG
                     NDIALKAIEEGFDVIPVPGANAAISALVASGLQTQPFTFFGFLSRQKKERNEQLQKLK
                     QKEETLLLYEAPHRLKETVKALSESFGGSRQVVLARELTKRYEEFLRGTLEEAVSWAE
                     SNEIRGEFCIVIAGNEDVEEASENWWQDISIQTHVEMVMEKEGISSKEAIKVVALERE
                     VPKRDVYQEYHVNGNV"
     gene            complement(6053..6340)
                     /locus_tag="VFX34_09420"
     CDS             complement(6053..6340)
                     /locus_tag="VFX34_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX5565170.1"
                     /translation="MDKMNEHLFYVLECNDGSYYAGYTNDLEKRIRTHNEGKGAKYTR
                     AKLPVRLIYRESYETKQEAMRAEYKFKQLARAAKEQYMREGQRNDEVTEKR"
     gene            complement(6324..7073)
                     /locus_tag="VFX34_09425"
     CDS             complement(6324..7073)
                     /locus_tag="VFX34_09425"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEX5565171.1"
                     /translation="MENLLKDDERLDYLLAENLRIIQSPSVFSFSLDAVLLAKFAYVP
                     IRSGKIVDLCAGNGAIPLFLSARTNADIIAVELQERLADMARRSVGYNGLDERITIVN
                     DDVIGIAGKIGYEKYDTVTCNPPYFPAIEASEKNLKEHVAIARHEIRLTLEQAVQSAS
                     ELLKQGGKAAFVHRPGRLLDIVTAMRANRLEPKRIRFVYPKEGKEANTLLIEGIKDGK
                     PDLKILPPLYVYGQDGEYTEEVRLLLYGQDE"
     gene            complement(7135..7494)
                     /gene="yabA"
                     /locus_tag="VFX34_09430"
     CDS             complement(7135..7494)
                     /gene="yabA"
                     /locus_tag="VFX34_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEX5565172.1"
                     /translation="MKEGNFLDRVMEFEQQLEAMHEQFRELIGFVAKMTEENHSLQLE
                     NHHLRTRLDELTDKTQLMDEKRPPEKPQRVDIGEGVDNLARIYNEGYHVCNVHYGSSR
                     KGEDCLFCLSFLSKQNA"
     gene            complement(7508..>7800)
                     /gene="ricT"
                     /locus_tag="VFX34_09435"
     CDS             complement(7508..>7800)
                     /gene="ricT"
                     /locus_tag="VFX34_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX5565173.1"
                     /translation="KMAKDQNLSLNPSKISGLCGRLMCCLKYENDEYEEAKALMPDIG
                     ERVLTPEGPGKVVGLNLLERILQVNLADQDQVLEYSWEEIESRKNSVAQLTK"
BASE COUNT         1918 a         2022 c         1553 g         2307 t
ORIGIN      
        1 ctcgcaaacg ccgttctccg taacggcgtt cacaggagtc gccgccttcc gcttcaatcc
       61 gctaaatcca tatcaatgat aataataata ataatataaa aggatgaaaa aaaccgtggg
      121 actggataag ccccacgggt cgatgtggaa ataaaatgat tgcgattaaa ttagtcgatc
      181 actttgattt tcactgtgcg acggccgaaa ttgtatgctg ccgcctttgt cggtacatga
      241 agatctatct tcttcccttt gatcgctccg cctgtatccc cagcgatcgc atatccgtaa
      301 ccttccaccc aaactttcga gccgagcgga attacactag gatctactgc gatgactttc
      361 agattcgggt tggctctcag gtctatacct gttgccgttt tccctgtgca accgttacag
      421 tgcgcagtat aagcagtggc ggtcacgtag aattccttcc cgccggacgg tgctgcagag
      481 ctgccgcgtg aaactgcgac gtttgttttc ttaatagagg cgctagccac aactactttg
      541 gaaccgaccg caacaatttt cttctgcggt tcctcaatga ctctttcact caataacgtg
      601 cgagatactt ccttgccatt ttctttgaca atttcaaact tgcgttcaac agaccccttc
      661 ttgccttctt ggacaacctt ctcgcgtcct ttcagtaatt tggcgtcgct tttcgttaca
      721 accgcaaagt ttgttggttc ttccactaca tcggtgacct tttctacccg tacaacttca
      781 attatggaac cgggcttcac gaatacgtcg gccttttggt tcagcttgtc gagttcattc
      841 aattgaatgt tctctttcct taaaaagtca gcgaccgtag tcgaagtgga ccacatttgt
      901 ttctcattcc cgccatcgat aagcgtcaat tggaacgctt tttgaatatt gatgtgtaga
      961 ttttccccca ggttttcgtt cagatttggt gtgacttcat catgctctgt aagctcgatg
     1021 ccagccttgg ctaaaatatc accgacattc ttatccgtcg tccaaatctt ttctgtgtcg
     1081 ttacctgttt gaatctctac ttgctttgcc tgttcccacc agatcgatat tccttcttcg
     1141 atcggggtat tcactgaggg tgttactaag tcgtgttcgg ctacttcgat atcctcactg
     1201 gaaagaagtt gcccaactgt atgtgcagat gtttttattt caatttgctc atcgttcaca
     1261 tgaagcgtga ccgatttctt ggatacttcg taaagaacaa gtgaaatgat tgaaacaaat
     1321 agcgcaaacg taaaagcaac gatcgctatt tgtttactcc tcaatgacat cgagaacaga
     1381 tttttcatgg tgctttttgg catgaaaaaa ctcctccttt atcactcggc aaattataag
     1441 gcttcctttt cgacgtgtca acccctttgg ttttcaactt tttccactag gggtggatga
     1501 attcaatcag gaagccgtac aatcggcttt cgtgttattt cggaatgcgg aatatcttca
     1561 aagcgttttc ggtcgttctt tttgctactt cttcaaccgg aatttccttt agtcgggcaa
     1621 tttcttctgc cacgagtgtg acgagcgccg gttcattccg tttgccgcga tgcgggtggg
     1681 gagcaaggta aggagcatcc gtttcaatca ataaatgatc caacggaatt tccattgcaa
     1741 cctgcttcgg cattttggca tttttgaagg ttacggggcc cccaagagaa atgaggaaat
     1801 tcatatcaat gcactctttc gcggtctcga cgctgccgcc atagcaatgc atgatcccgc
     1861 ccgttattcc ggcttcttct tccttcaaaa tcctgacgac atcccctgtt gcatcacgat
     1921 tatggatgat gactggcaga ttgacctttt gtgcaagccg gatctgcttt cggaacaatt
     1981 cctgctgaat gtccttcgga gatttatccc aataatagtc caggccggtt tcgccgatgc
     2041 cgacgacttt cggatgcgct gccaaggact cgatccattt cagatcttcc tcggtgcaat
     2101 cgacagcatc tacaggatgc catccgatga ctgcgtaaat gaatggatag tgctcagcaa
     2161 gctccatcgc cttttcgatc gtcacacggt cgaagccgac aacgaccatt ctttcgaccc
     2221 ctgcttcaag tgcgcgttga atgacttctt ttacgtcttc ttcatattgg tctgcgttca
     2281 aatggacatg agtatcgata tacattttcg tctctcctca caaacttcat tctatttcaa
     2341 tgaaatatga ctttcgttac agtatggtga caattcagtt aaaagataaa gacggcgctt
     2401 cataagaaac gccgtcctgt atgattcact tcacttgtgc cccgttttcc agcgttgcat
     2461 caacggttgc taatttcaat acgccgttgg aactgcctgc caaaatcatt ccttgggaca
     2521 attcgccgcg taatttcacc ggctttaagt tggcaacgac aataaccttc tcgccgatca
     2581 acgcttccgg ttcataatgt tcggcgatgc ctgaaacgac ctgacgctgt tcatagccca
     2641 aatcgacttg aagcttcagc agcttgtccg ctttcggaat cttctcacat gccgtaacgg
     2701 tcgcgacacg caaatcgact ttcatgaagt cgtcaatcgt gatttcttcc gcttcctctt
     2761 cggctttgac cggctcttgc tcctgctttt cctgtggcgc tgtaatcgcc atctggtcac
     2821 ggatatagac gatttccact tcgggatcaa ggcgagggaa aatcggaatc cctttttcca
     2881 ccactttcat cccttcagga atcgcattga aatcacttaa tgtttcccaa gcgagcatct
     2941 tgtcatctaa cccgagctgt tcgatgatct gaatcggcgc cttcgtcatg aacggctgca
     3001 agagcaccgc gatttgtcgg agcgatgcag caagatgggc catgacagat gccaatttcc
     3061 ctttatccgc ttcttctttt gcaagaaccc aaggcgaggt ttcatcgata tatttattcg
     3121 tacgggaaat gattgtccat agctccgata agacaacgct gaattgcatc ttctccatcg
     3181 cagcttcgta tttctcaatt gcgttcgtcg tgaaatcgcg cagtacactg tcgaattcag
     3241 tcggctcgag cccctctgtt ggaatgacac cgtcgaaata cttattgatc atggaaactg
     3301 tccgattcaa caagttgcca agatcattgg ccaagtcgta attcgtccgc tcgacgaacg
     3361 actcaggtga gaatgtgcca tcttgtccga acggcaattc acgaaggagg aaatagcgcg
     3421 tcgcatccaa tccgtatcgc tccacgagca tttcaggata gacgacattc cctttcgatt
     3481 tcgacatttt gccgtccttc atcataatga agccgtgggc gaacactttt ttcggcaatg
     3541 gaagatcgag cgccataagg aaaatcggcc aataaatcgt atggaaacgg acaatgtcct
     3601 tcccaacgac atgcacatct gctggccagt atttattgaa tagcgtttca tcatcggagc
     3661 cgtatccgag tgcagtgatg taattcgtca atgcatcgac ccacacgtaa atgacatgct
     3721 tcggatctcc aggcacttta atcccccagt cgaatgacat acgggagacg gataagtctt
     3781 caagtcccgg tttgatgaag ttattgatca tctcgttttt ccgggattcc ggttcgatga
     3841 aggtaggatt gtcttcatag tactgcagaa gcctgtccgc atacttcttc atattaaaga
     3901 agtacgattc ctccgaaacc ctttcaaccg gacgatgaca atccggacaa ttgccatctt
     3961 caagctgcgc ctccgtgaag taggattcac aagggacaca tttccaacct tcatattcgc
     4021 ctttataaat gtcgccattg tcgaggaact tctggaagat tttctcgaca gctactttat
     4081 gccgttcctc cgttgttctg atcagatcat catacgagat gtccatcagc ttccacaggt
     4141 cgatcgcgat atccgcgata cggtctacgt actgctgcgg cggtagacct tcctcttcgg
     4201 ctttctcctg tattttctgt ccgtgctcgt ccatccccgt caggaaacgt acgtcatatc
     4261 cacgcaatct tttatagcgg gccatcgcat ccgatgcaac ggttgtataa gcagttccaa
     4321 tatgaaattt cccactcgga taataaatag gagttgtaat ataaaatgtt ttttgctgtt
     4381 ctgccaccaa aatacctcct gtgtttcaat ctatactaac catattactt aaatttcaac
     4441 aaaagcgcta tgcagacacg cataaaatcg tcaatacgcg cataaatccc ttgaaacaag
     4501 cataaatcgt ctaaaatgct cataaacgtt tccaacggcg cataaccgcc cgcaaatgct
     4561 cataaaccgc tccaaatgcg cataaatgca atatttcaca cattacaaaa tttccttatt
     4621 agtgctcaaa tgctctttca cttccatttc cctaaatcct atccaatttg gatacttaat
     4681 acctcacaaa gcatgataaa attaaacatc tatagtccca ttttcttcaa ttaatctatt
     4741 attctacaca taatattcgc gggtcttatt gacgttaaat ggaagagttg gtatgatatt
     4801 tgatagacaa gagaaatatg tcgaattttg acgaaaattt aaatcgttta ggaggaattc
     4861 ttgtcatgaa atctacagga atcgtgagaa aagtcgacga gcttggccgc gtcgtcattc
     4921 caatcgaact tcgccgtaca cttggaatcg agcagaaaga tgctttggaa atctatgtcg
     4981 atgatgacaa aattatcttg aagaaataca tgccaaacat gacttgttct atcacaggtg
     5041 aagtttccga caataacttg cagcttattg acggaaagct gattttaagc ccggatggtg
     5101 ctaaacaact cattaaagaa atcgaagaaa agataaaata atattatctt tgcaataata
     5161 tttaaatcca gaacaagtaa ctgttctgga tttttattat acattcccgt tcacatgata
     5221 ctcctgataa acatcccttt ttggcacttc tctttccaat gccactactt tgatcgcttc
     5281 ctttgatgaa ataccttcct tttccatcac catttccaca tgcgtctgaa tgcttatatc
     5341 ctgccaccag ttttcgcttg cttcttcaac atcctcattg cctgcaatga cgatacagaa
     5401 ttcccctctt atttcattcg attccgccca agaaacagct tcctcaagcg ttccgcggag
     5461 aaattcttca tatcgtttcg tcagttccct tgccaacacg acttggcgcg aaccgccgaa
     5521 tgattccgaa agggccttga ccgtttcctt caagcgatgg ggggcttcat acaggagtaa
     5581 agtctcctcc ttttgcttca gtttctgcag ctgctcattg cgctcttttt tctgccgcga
     5641 tagaaagccg aagaaggtga atggctgcgt ctgcaatccg gaagcaacca atgcactgat
     5701 ggcagcgttc gcacctggga ctggaatcac atcaaaaccc tcttcaatcg ctttcaaagc
     5761 aatatcattt ccgggatcgg atatgcatgg catccctgcg tcactcacaa gggcgaccgt
     5821 cttcccttcc gccaatagcg acaggatttt ctctccgccc ttctcgagat tatgctcatg
     5881 gtagctcata agcggtgttt ttatctcgaa gtggttgcaa agccttattg tattcctcgt
     5941 atcttccgct gctatcatat ccgcttcctg caggatccgg attgcccgga atgtcatgtc
     6001 ctccaaattg ccgatcggcg tcccgactag aaatagcttg gcaacaccgg tttcagcgct
     6061 tttctgtgac ttcatcgttt ctttgcccct ccctcatata ttgttccttt gctgctcgag
     6121 ccaattgttt gaacttgtat tcagccctca tcgcttcctg cttcgtttcg tagctttccc
     6181 ggtaaatgag gcggaccgga agcttggcac gcgtatattt tgcacccttt ccttcattat
     6241 gggtacggat gcgcttttcc aaatcattcg tatagcctgc atagtaggaa ccgtcattgc
     6301 attcaaggac atagaacaga tgctcattca tcttgtccat ataataaaag cctcacttct
     6361 tccgtatact ccccgtcctg gccatacaca taaaggggcg gtaatatttt taaatccggc
     6421 ttgccgtcct tgatcccttc tatcaataac gtgtttgcct ctttgccctc tttcggatac
     6481 acgaaacgga tccttttcgg ttccaaccgg tttgcacgca ttgccgtcac aatatcaagc
     6541 agcctgcctg gacgatggac gaatgccgct ttcccgccct gcttcaacag ctcactcgca
     6601 gactgtacgg cctgttcaag cgttagcctg atttcgtgcc ttgcaatggc gacatgctcc
     6661 ttcaagtttt tctcactcgc ttcgattgca ggaaaatagg gtggattgca agtgaccgtg
     6721 tcatactttt catagccgat cttcccggcg ataccaatca cgtcgtcatt cacgattgta
     6781 atccgttcat ccaatccgtt atagccgaca cttctccgag ccatatcagc aagcctttcc
     6841 tgaagctcga ccgcaataat atcagcgttc gtccgggcac ttaggaaaag gggaatcgcc
     6901 ccgttgcctg cacataaatc gacaatctta cccgaacgga tcggcacata tgcgaatttt
     6961 gccagcaaga ctgcgtcaag tgaaaatgaa aagacggagg ggctttgaat gatccgcaag
     7021 ttctccgcca atagatagtc cagccgttca tcatccttca ataaattctc cattcagttc
     7081 tctccaatca aaagaaaaaa gccgatgcct acatgattat aggatcggct catctcaagc
     7141 attctgtttg cttaggaagg ataggcagaa caaacaatct tcgcctttac ggctgcttcc
     7201 gtaatggaca ttgcagacgt ggtatccttc attatatatc ctggccaggt tgtccacccc
     7261 ttcgccgata tcgactcttt ggggcttctc aggcggccgt ttttcatcca tcaattgagt
     7321 tttgtcagtc aattcgtcaa gccgcgtccg gagatgatgg ttttccagtt gaagtgagtg
     7381 gttctcttcc gtcatcttcg cgacaaatcc aataagttcc cggaattgct catgcatggc
     7441 ctcgagctgc tgctcaaact ccatcactct gtcgagaaag ttcccttctt tcaactattt
     7501 caccccatca tttcgtcaat tgagctactg agttttttcg actctctatt tcttcccatg
     7561 aatactctaa gacctggtcc tgatccgcca aattcacttg caaaatccgc tcaagcaaat
     7621 tcaagccgac cactttcccc ggcccttcag gcgtcagaac ccgctcgcca atatcaggca
     7681 taagggcttt cgcctcttca tattcatcgt tttcgtattt caagcaacac atgaggcgac
     7741 cgcataatcc agagattttg gatggattga gtgaaaggtt ctgatccttc gccatcttga
//
LOCUS       DASULI010000434         5285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_538797, whole
            genome shotgun sequence.
ACCESSION   DASULI010000434 DASULI010000000
VERSION     DASULI010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538797"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /gene="dsdA"
                     /locus_tag="VFX34_09440"
     CDS             complement(<1..1085)
                     /gene="dsdA"
                     /locus_tag="VFX34_09440"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HEX5565174.1"
                     /translation="MNETEKAEWIERLPLLRKIIEKQEVFWRNPLNSDSAAGIAQSGI
                     SAADVKDASDRLRRFAPYIAKVFPETAEANGIIDSPITAIPEMKEALAAAYETAIPGE
                     LLLKQDNALPISGSIKARGGIYEVLKHAETLAVEHGLIQIDDDYTKFAGPEFRNLFAK
                     HKIAVGSTGNLGLSIGIMSAKLGFNVTVHMSADAKQWKKDLLREKGVTVVEYADDYSK
                     AVEEGRRQAEADPLCHFVDDENSLDLFLGYAVAGERVAAQLKERGKIVDEDNPLFVYL
                     PCGVGGGPGGVAFGLKLQFGNNVHCFFAEPTHSPCMMLGMMTGLHEEVSVADFGLDNQ
                     TAADGLAVGTPSGFVGKKMEPLLEGCYT"
     gene            complement(1140..2003)
                     /locus_tag="VFX34_09445"
     CDS             complement(1140..2003)
                     /locus_tag="VFX34_09445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5565175.1"
                     /translation="MKKILAIVFIAVLAIFFLIKDKDARKEADPLYTEAKAAIVLQAD
                     SGKILYEQNAKEALPIASMSKLMTQYIVLEAIKDGHLSWDSVYKPSTAALNQSRHAVI
                     LGMDKHKTYNVRELFAAMTITSANDAAVALAEMVSGTEKEFVKEMNKKAKQFGLRDTR
                     FINATGLDEDETNRATARDVAAIANELLTQYPEVLEFTRMTDFTTSEGARLWSTNLML
                     PGMPQEMTGMEGLKTGYTEEAGPCFASTGIYDGKRIITVVIGVEPEGSDTSTPRFELT
                     RDLIDRYVLNK"
     gene            2334..4367
                     /locus_tag="VFX34_09450"
     CDS             2334..4367
                     /locus_tag="VFX34_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565176.1"
                     /translation="MESAGILAGPMLRRVDTDSVAIWVATDCPVEVDAAIYVLDTLFQ
                     LDTATKTTSIRAGHRLFIHLVQVHGQFPMETLLGYDLLFRVGKTMNNLASLGLINNKE
                     DSITYDGLPYPSFFIPASAAPTFLYASCRKFHGKGDDALVAADAILQRETRNLAARPC
                     ALFLTGDQIYADDVADPLFPVIRTVAEHYIGKEDLGQFDERLKEEPFHTALWQVNGRQ
                     FITERFCRFTSGSASNHLLTFGEYAAMYLLSWSPAMWEGVFLPSFDEVVKKNELYVYL
                     PEDRDELKQCRVRFEEQTGQLLQSIGSLHRIRRLFANMPTYMMFDDHDVTDDWNLSAD
                     WRSSVLASPLGKHVIANGLCAYWAFQGWGNEPQRFGEGFLQSMCSHFDGFIADSTAYR
                     VWENRLWSFKGWQFVAPTTPAALFLDTRTMRHFEQEPKPVKVGRMYKETVQTPRLIGP
                     SGWKVTARTLLKSRWTRGDPLIIVSPTPLYGLGLIESALHSYVYPLRALGYPIHEVLD
                     FEAWKYNGRGFTDFIGQLFHWSLSRCIILSGDVHYASAVRSRLTSKREVLDILQFTSS
                     PSHNMSFAGVWGKLMKMAVRGNARRRKKTIYRYCDVGFSIQSGGKAIDCPKDAKWQEA
                     LRYLKMQGGTIVETENNIGMVTVGEDVIRHSLLQMKRGHMKEIRFEEMEATPE"
     gene            complement(4611..>5285)
                     /locus_tag="VFX34_09455"
     CDS             complement(4611..>5285)
                     /locus_tag="VFX34_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016129604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX5565177.1"
                     /translation="ELWMKLILHELNTAIELIEKDELPEAFKMLARVSRVQTQIIQAW
                     DVLSTLTPAEYMEFRESLGRASGFQSYQNRLIEFALGYKQPHILKIYEKDPELANELK
                     TAFEAPGIYDVSIRALARAGFPINPDLLTRDFSITDAGDPTVASAWLEVYRDVDRYWD
                     LYQLAEKLVDIEDSHQQWRFRHMKTVERIIGFKTGTGGSSGVNYLKSVLDHKFFPELW
                     DVRTKL"
BASE COUNT         1167 a         1305 c         1434 g         1379 t
ORIGIN      
        1 gtgtagcagc cttctaggag cggttccatc tttttgccga cgaatccgga tggcgtgccg
       61 accgcgagcc catccgccgc ggtttgattg tcgagaccga aatcggcgac ggatacttct
      121 tcatgcaagc ccgtcatcat gccgagcatc atgcatgggg aatgcgtcgg ctccgcgaag
      181 aaacaatgga cattgttgcc aaattgaagc ttcaggccga aagcgacgcc gcccggtccc
      241 ccgccgacgc cacatggcaa gtagacgaat aacggattgt cttcatcgac gatcttgccg
      301 cgttctttta attgcgcagc gaccctttcg cctgcgacgg catagccgag gaaaagatca
      361 agggagtttt catcgtctac gaagtgacag agcggatcgg cttccgcctg gcgtctgcct
      421 tcctccaccg ccttgctgta atcatccgca tattcaacga ctgtcacgcc tttttcccgc
      481 agaaggtcct tcttccactg ttttgcatca gccgacatat ggacggtgac gttgaagccg
      541 agcttcgcgc tcatgatgcc gatgctcaac ccgagattcc ccgtcgagcc gacagcaatt
      601 ttatgcttcg cgaataaatt tcggaattcc ggccccgcaa acttcgtgta gtcatcgtct
      661 atttggataa gtccgtgctc aacggcaagc gtttcggcat gcttcagcac ttcgtaaatg
      721 ccgccacgcg ctttgattga cccagatatc ggaagagcat tgtcctgttt caacaataat
      781 tccccgggga tggccgtttc atatgcggca gccagtgctt ctttcatttc cggaatggcg
      841 gtgatcggtg aatcgatgat gccatttgct tcagctgttt caggaaatac ctttgcaatg
      901 tacggtgcaa aacgccgcaa ccggtcactt gcatccttca catccgccgc gctgatgccg
      961 gattgggcaa tgcccgctgc actgtcggaa ttcaacgggt ttcgccagaa cacttcctgt
     1021 ttttcgatga tctttcgcag taatggcaat ctttcgatcc attcagcctt ttcggtttca
     1081 ttcacggtta tcggccccca attaaaagaa tttccatcat tatagcatgc aggaagcctc
     1141 tatttattca aaacatagcg gtcaatcaaa tcacgggtca actcgaagcg gggagttgaa
     1201 gtatcgcttc cctccggctc gacgccgatg acgaccgtga tgatccgttt tccgtcatat
     1261 atgcctgtac ttgcaaaaca aggtcccgct tcctccgtat aacccgtttt caatccttcc
     1321 atgcctgtca tctcttgcgg cattcccgga agcatgaggt tggtgctcca aagccttgca
     1381 ccttcgctcg tcgtgaaatc tgtcatgcgg gtgaattcca gcacttccgg atattgcgtc
     1441 agcagttcgt ttgcgatggc tgcgacatcc ctggcggtcg cccgattcgt ctcgtcttca
     1501 tccaatccgg ttgcattgat gaagcgggtg tcccgtaagc cgaattgttt ggccttcttg
     1561 ttcatctctt tcacaaattc cttttctgtg ccgctcacca tttcggcgag cgcgacagct
     1621 gcatcgtttg ccgaagtgat tgtcatagcg gcgaaaagtt cgcggacatt gtatgttttg
     1681 tgcttatcca tccctaaaat gacggcatgc ctcgattggt tcaatgccgc tgtggatggc
     1741 ttgtatacgc tgtcccatga aagatggccg tccttgatcg cttccaggac gatgtactgg
     1801 gtcatcagct ttgacatgct agcgatcggc agcgcctcct tggcattttg ctcgtagagg
     1861 attttgccgg aatccgcttg aaggacgatg gctgcctttg cctcggtgta gagtggatct
     1921 gcctctttac gggcgtcttt gtctttaatg aggaagaaaa tcgccagaac agcgataaat
     1981 acgatggcga gaattttttt catatgggaa atcacacctt ttcctttgtt acttctattc
     2041 tctacaacaa agatgaagat tcatgcatgc attttctgaa gaatttctga agatggtaag
     2101 ggtgaaaagt cccgaaacga gcataacttc gatagtttgc gccataacag gcccgaactc
     2161 gagcataact ccgcccaatt gcgccatacg ttcgccgaaa tgcgacataa ctcccctgga
     2221 ctcgagcaaa accctagaca tagcggcaat tagggcattt ccccccgcac acaccttcat
     2281 tatccggact gaatacgcta gtagtaataa cgtcagtgat ggtggtgatt tgcatggaga
     2341 gtgcggggat tttagcaggg ccgatgttac ggcgggtgga tacagattcg gttgccattt
     2401 gggtggcgac ggactgtcct gtcgaggtgg acgctgccat ttatgttctt gacacgcttt
     2461 ttcagcttga caccgccacg aaaacaacat ctatacgggc aggccatcgt ttgttcattc
     2521 accttgtgca ggtgcatggg caatttccaa tggagacact ccttggctat gacctgcttt
     2581 ttcgcgttgg aaaaaccatg aataatttgg caagcctcgg attgattaac aacaaggaag
     2641 attccatcac atatgacgga cttccctacc cttcgttttt catccccgct agtgcagctc
     2701 caacttttct atatgcatct tgtcggaaat tccatgggaa gggagatgat gccctcgttg
     2761 cggcagatgc aattttgcaa agagaaacga ggaatcttgc ggcacggcct tgcgctttgt
     2821 ttcttaccgg tgaccagatt tacgcggatg acgtagcgga tcctttgttt cctgtcatca
     2881 gaacagttgc cgaacactat attggtaagg aggaccttgg gcagtttgat gagcgtctga
     2941 aggaagagcc gtttcacacc gccctttggc aagtgaatgg acggcaattc attacggaac
     3001 ggttttgccg gttcacttca ggcagtgctt cgaatcatct gctcacattc ggggagtatg
     3061 cggcgatgta tttgctgtct tggagtccgg cgatgtggga aggcgttttt ctcccttcgt
     3121 ttgatgaagt tgtgaaaaag aatgagttat atgtttatct tccggaggat cgtgatgaac
     3181 tgaagcagtg cagggtccgt ttcgaagagc agaccgggca gctgctgcag tcaatcggtt
     3241 cgcttcatcg gattcgcagg ctttttgcga atatgcctac gtatatgatg ttcgacgacc
     3301 acgatgtgac ggatgactgg aatttatccg cagattggcg tagttcggtg ctcgcttcgc
     3361 ctctaggaaa acatgtgatt gcgaacgggc tctgtgcgta ctgggcgttc caaggttggg
     3421 gcaatgagcc acaacggttc ggggaggggt tcctacagtc gatgtgtagt catttcgacg
     3481 ggttcattgc tgactcaact gcttatcggg tttgggagaa tcggctttgg tctttcaaag
     3541 gctggcaatt cgtagcgccc actacacctg ctgcgctatt tttagatacg aggacgatgc
     3601 gtcattttga gcaggagccg aaaccggtga aagttgggcg gatgtataag gagaccgtcc
     3661 agacgccgag attgatcggc ccttcaggtt ggaaggtgac agcgcggacg cttttgaaat
     3721 ccaggtggac acggggggat ccgctgatta tcgtttcccc gacgccattg tatgggttgg
     3781 gactgatcga gtccgcactc cattcgtatg tgtacccgct ccgggcgctc ggttacccga
     3841 ttcatgaagt gctcgatttt gaggcgtgga agtataacgg cagaggattc accgatttca
     3901 tcggccagct gttccattgg agcctgtccc ggtgcatcat cctgtcgggg gatgtccatt
     3961 atgcatctgc ggtgcggtcg cgactgacgt cgaaacggga agtgctggat atattgcaat
     4021 tcacgagcag cccttcccat aatatgagct tcgcgggcgt atggggaaag ctgatgaaaa
     4081 tggcggtccg gggaaatgcg aggcggcgga agaagacgat ctaccgctac tgcgatgtgg
     4141 ggttttcgat tcaaagcggc gggaaggcga tcgattgccc gaaggacgcg aaatggcagg
     4201 aggcgctccg ctatttgaag atgcaaggcg gtacgattgt cgagacggag aacaatatcg
     4261 ggatggtcac tgtcggggag gatgttatta ggcattcgct gttgcaaatg aaaagaggac
     4321 acatgaagga gatccgattt gaagagatgg aagccacgcc ggagtgagcc ggcggggctt
     4381 ttttgcgttt tgcgcagatt taggtgagtt atgtgcgagt ttgaaggagt tatgcgcgaa
     4441 atcggaagtg ttttgcgcgg atttggacta gttatgcgca tattcgatgg agttatgatc
     4501 atttcgggac tttcggaccg gtcggtggac gtggagcaac aggataatgc gcagctgcca
     4561 acactggcga cgcgattcaa aaaaaagaga ctacccaagc ggatagcctc tcacaacttc
     4621 gttctaacat cccatagctc ggggaagaat ttatggtcga ggacggactt caaatagttc
     4681 acgcctgatg aaccgccggt gccggttttg aatccgatga tccgttccac cgtcttcatg
     4741 tggcggaagc gccattgctg gtggctgtct tcgatgtcga cgagcttttc tgcgagctgg
     4801 tacaggtccc aatagcggtc gacgtcacgg tatacttcaa gccacgcgga tgccacggtt
     4861 ggatcgcccg catcggtgat cgagaaatcg cgtgtcagca aatcgggatt gatcgggaat
     4921 ccggcgcgtg caagtgcacg gatggagacg tcgtaaatgc ccggcgcttc gaaggccgtt
     4981 ttcaattcat tcgctagctc agggtctttc tcgtagattt tcaaaatatg cggctgcttg
     5041 tagccgagcg caaactcaat gagccggttc tggtaagact ggaagccgga tgcgcgacca
     5101 aggctttcgc ggaactccat atattcggca ggtgtcaatg tcgaaaggac gtcccatgcc
     5161 tggatgatct gcgtctgcac ccgggagaca cgcgccagca ttttgaacgc ttccggcagt
     5221 tcgtcttttt caatcagctc gattgccgtg ttcagctcat gcaagatgag tttcatccaa
     5281 agctc
//
LOCUS       DASULI010000435         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_63860, whole
            genome shotgun sequence.
ACCESSION   DASULI010000435 DASULI010000000
VERSION     DASULI010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63860"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFX34_09460"
     CDS             <1..263
                     /locus_tag="VFX34_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5565178.1"
                     /translation="RVRPIFMTTLTTVGGMLPLALASGASGNYQAPLATVLISGLLFA
                     TFITLLLIPSVYRLFMKTEKVAEAATFKHKKRVATRLAEPVK"
     gene            260..382
                     /locus_tag="VFX34_09465"
     CDS             260..382
                     /locus_tag="VFX34_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565179.1"
                     /translation="MKYGSESTIFMKMGIVFFRLSSGKKDGYNEAMKKGSELVE"
     gene            375..983
                     /locus_tag="VFX34_09470"
     CDS             375..983
                     /locus_tag="VFX34_09470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5565180.1"
                     /translation="MNEKEYDNLLRIKTGGTLELLSQSAHYNRYEATPYEALDELFKS
                     YELHQSDGFVDFGCGKARVSFYVHHHFGASVTGIEMNGQLYQDALENLARYRRKAKHH
                     GGAIRFERCFAETYDIDPSENKFYFFNPFSIQIFMTVIGNILISVEQAARPVDIILYY
                     PTAEYVQFMDNQTPFWLWKKVKIDRLYDKNVNERFLIYRLGE"
     gene            complement(1131..3068)
                     /locus_tag="VFX34_09475"
     CDS             complement(1131..3068)
                     /locus_tag="VFX34_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor GTPase family protein"
                     /protein_id="HEX5565181.1"
                     /translation="MHKTIGVVAHVDAGKTTFSEQLLFHTHSIRQRGRVDHKDTFLDS
                     HEIEKNRGITVFADQGAFTFNGSHYTLIDTPGHVDFSPEMERAIQVMDAAIVIISAAD
                     GVEGHTETVWQLLKKHRVPTFFFLNKTDREGVDLQEVVDEIRGSLSENIFDLSEMKDG
                     IMSESLIEFIAERDEDLLERYMDAGYDREMWLDVMKRMIKRRGIFAYGHGSALKDVGV
                     LEFLQKFDLLTVTEYTDADDFTARVYKVRHDENGNRVTFLKVLQGILHVRDEVQIAGK
                     LEKITQIRRYNGMKFQTLEAAHAGEIVAVTGLSEAGAGDPIGALFMKAEFDLIPTLKS
                     KVIFNSSIHVKEVLRCFNMLDAEDPSLRVVWDEHVQEVHVHVMGVIQLEVLKQIVRDR
                     FHFDVSFGDPKILYKETIRTAVNGYGHFEPLKHYAEVHLKIEPAARNSGISFENICHA
                     NDLSIGNQNLVRTHIFEKAHHGLLTGSALTDVKITLLTGKGHNEHTSGGDFREATFRA
                     LRQGLEKAENVLLEPFYDFKIKVGMDHIGRVLSDIQQASGRFAPPETIGETTVITGRV
                     PVATFMNYSTTFASYTNGKGALTLQFGGYDECHNPEEVIEAIGYNKDADPEYTSTSIF
                     CAKGKGYPVPWYEAEAAMHLL"
BASE COUNT          921 a          902 c          857 g          933 t
ORIGIN      
        1 acagggttcg tccaatcttc atgacgacgt tgacgactgt cggcggaatg cttccgcttg
       61 cgctcgcttc aggcgcatcg ggcaattatc aggcgccgct cgcaacggtg ttgatttcag
      121 gattgctgtt tgcgacgttc atcacgctgt tgctcattcc ttcggtgtat cgtttgttta
      181 tgaaaacaga gaaagtggcg gaagcggcga ctttcaaaca taaaaaacgc gtagcgacac
      241 gattggctga acctgtgaaa tgaaatatgg tagtgaaagc acgatcttca tgaaaatggg
      301 gatcgtgttt ttccgcttgt catccggcaa gaaagacggc tacaatgagg cgatgaagaa
      361 agggagtgag ttggttgaat gaaaaggagt acgacaacct tcttcggata aaaacgggtg
      421 gaacgctgga attgctcagc cagtctgcac attataaccg ctatgaagct acgccctacg
      481 aagcgttgga tgaactgttc aaatcatacg agctacatca atcggacggg ttcgtcgatt
      541 tcggctgcgg caaagcgcgc gtgtcatttt acgtccatca tcatttcggg gcatctgtaa
      601 cgggcattga aatgaatggc cagctctatc aggacgcgct tgaaaacttg gctcgttatc
      661 ggcgaaaagc gaagcaccac ggtggagcga tccgttttga acgatgcttc gcggaaacgt
      721 atgacatcga tccatcggag aataaatttt actttttcaa tccgttttcc attcaaatat
      781 ttatgacggt tatcggaaat atcctgattt ccgtggaaca ggctgcgcgc ccggttgaca
      841 tcattcttta ttacccgact gcggaatatg tgcaatttat ggataaccag acgccgtttt
      901 ggctttggaa gaaagttaag attgaccggc tctacgataa aaatgtaaat gaacggtttt
      961 tgatctatcg attgggtgag taggggagat atcaatcatt ttgttacttc ggacctgatt
     1021 ggtcaaatgc attcggcgcg aatgggctgg tcgtttgcga tgaatatgac cgcggaactg
     1081 aaaatgcagt agcccttacc cgtcgcgcct agtgccttta acaaatacaa ttacagcaaa
     1141 tgcatcgctg cttccgcttc ataccacgga acaggatagc ctttgccttt cgcacaaaaa
     1201 atcgacgtgg atgtgtattc agggtcggcg tctttgttat agccgatcgc ctcgatcact
     1261 tcctctggat tatggcattc gtcatagccg ccgaactgca aagtgagtgc acctttgccg
     1321 ttcgtgtacg atgcgaaagt tgtgctgtag ttcataaatg tggcaaccgg aacacggcct
     1381 gttatgacag tggtttctcc gatcgtttcg ggcggtgcaa aacgtcccga tgcttgctgg
     1441 atatcggaga ggacacggcc gatatgatcc attccgactt ttattttgaa atcgtaaaag
     1501 ggttccagca agacgttttc cgctttctcc agcccttgcc gcaatgcgcg gaatgtcgct
     1561 tccctgaaat cgccaccgga agtatgctca ttatgcccct tgcctgtcag caaggtgatc
     1621 ttcacatcgg tcaacgctga tcctgtcaat aaaccatgat gcgccttctc gaaaatatgg
     1681 gttcgcacca agttttgatt gccgatggat aaatcattcg catgacatat attttcgaac
     1741 gaaatgccac tgtttcgcgc agccggttcg atcttcaaat ggacttccgc ataatgcttc
     1801 agcggctcga aatggccgta gccgttgaca gcggttcgga tcgtctcctt atacaagatt
     1861 ttcggatcgc cgaacgatac gtcgaaatgg aaccgatccc ttacgatctg cttcaatacc
     1921 tcgagctgga tgacgcccat cacatggacg tggacttcct ggacatgctc gtcccagacg
     1981 acgcgcaacg aagggtcttc agcgtctagc atgttgaagc accgcaatac ttcctttacg
     2041 tggatggaag agttgaagat gacttttgac ttcaacgttg gaatcaagtc aaactccgcc
     2101 ttcataaaga gagcgccgat tggatcgccc gcacctgcct ccgacaaccc cgtaactgca
     2161 acaatttccc ccgcatgagc agcttccaac gtttgaaact tcattccgtt atatcttcta
     2221 atctgtgtaa ttttctccag cttgccggca atctgcactt catctctgac gtgcaaaatc
     2281 ccttgcaaca ccttcaagaa cgttacccgg ttgccgtttt catcgtgccg cactttatag
     2341 acgcgcgcgg taaaatcatc tgcatccgta tattctgtta cggtgagcag gtcgaacttc
     2401 tgaagaaatt cgaggacgcc gacatccttt aaagcagagc catgcccata tgcgaagatg
     2461 ccgcggcgtt taatcatccg tttcattacg tctagccaca tctctctatc atagccggca
     2521 tccatatagc gttccagcaa atcctcgtcg cgttccgcaa tgaattcaat aaggctttcg
     2581 gacatgatgc catctttcat ttccgataaa tcaaagatgt tctctgacag gctgccgcgg
     2641 atttcatcca caacttcctg cagatccacg ccttcccgat cggttttatt caaaaagaag
     2701 aaagtcggga cgcgatgctt tttcagcaat tgccaaaccg tttcggtatg cccttccacg
     2761 ccgtccgccg cactgatgat gacgatggct gcgtccatca cttgtatggc ccgttccatt
     2821 tcaggtgaaa agtcgacgtg ccccggcgta tcgatgagcg tatagtgcga gccattgaat
     2881 gtgaaggcac cttggtctgc gaagacggtg atgccgcggt tcttctcaat ttcatggctg
     2941 tcgaggaacg tatctttgtg gtcgacgcgg ccccgctggc gaatgctatg cgtatgaaaa
     3001 agcagctgtt cggaaaaagt cgtcttgccc gcatcgacat gggcaacgac tccaattgtc
     3061 ttatgcataa gtgcacctcg attcatttct aactataatt ttaggcgtaa atgggaggga
     3121 agtgaaggtg taagttgatg agtccaaagg aacaagacga gcaaaatcca ctcgaactcg
     3181 agcataactc cggaagattg cgccataacc gcctcaaact cgagcataac tccgcgagat
     3241 tgcaccaaaa cccctccaaa ctcgagcaaa agattggtag attgttaaaa gcatttggcg
     3301 cgagtttgtg attcagggga agtggtggat ttccgcgttt ttggtagagg gtccaaagtc
     3361 ccgaaatgat cataactcgc ccaaaaacgc gcataactct cttcaattcg cgcataacca
     3421 gtccaaaata tcgcataacc gccaccaatt cgcgcaaaac agaccaaaat ttgctcaaaa
     3481 ccatatcctg ccacgccaaa agaaaaacgc accccatgtc ggagtgcgcc ccatcaaacc
     3541 gtgtgaaaca gcgattgtac gatttccttt tcaggcagcg gcttggaata gaggtagccc
     3601 tgcacttcat tgc
//
LOCUS       DASULI010000436         5739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_409884, whole
            genome shotgun sequence.
ACCESSION   DASULI010000436 DASULI010000000
VERSION     DASULI010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409884"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1342
                     /locus_tag="VFX34_09480"
     CDS             146..1342
                     /locus_tag="VFX34_09480"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5565182.1"
                     /translation="MNIDKKPVDRFDWPLAFILLLFATISIIAIASAQTTGQYKIDSI
                     PVNFIPSQIQWYAIGSIIIALTMYLEPEQYKKAAWIIYGGGVFLLVMLFILPEGMELV
                     SRRNGAKSWFHLPGIGSIQPAEFMKTFFIIGMARTITKHHERYLEKTLQTDFLLLGKI
                     AAVFLLPFSFIMLQPDLGTGLVFIAITVALIIVAGITWRIILPIFLGTAALGGTLLWM
                     ALYAQEFLAKFGFKPYHFERIYSWLDPYSYASNEGRHLITSLNAIGSGEIFGKGFKGR
                     EVYVAESHTDFIFSVIGEDWGFIGASLVICLYFFLIYHMTKITLELKDPFSTYICAGI
                     IAMITFHVFENIGMTTQLLPITGIPLPFISYGGSSLMGNMLAIGLVFSMKFHHRTYMF
                     SSDDEE"
     gene            complement(1730..1870)
                     /locus_tag="VFX34_09485"
     CDS             complement(1730..1870)
                     /locus_tag="VFX34_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004232019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lmo0850 family protein"
                     /protein_id="HEX5565183.1"
                     /translation="MAKEKVDLKKIISNLAKLGVSATATKSRMEMLKALAPPANDPQI
                     QS"
     gene            2317..3477
                     /locus_tag="VFX34_09490"
     CDS             2317..3477
                     /locus_tag="VFX34_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5565184.1"
                     /translation="MKKSILLLMSVQFFVYLGFGIIIPVLPEVIVQQDLSEIHVGGLL
                     TVYSLASFFTAPLWGSLSDRTGRKKLIIIGLIGFSLSFFLFAFFIHNLTLLYLSRVVG
                     GIFSGALYTAVTGFVGDMTTEENRNKYMGLLGMSIGLGFIFGPAIGGVLGHYSLQLPF
                     IASGVLTLLLVIYASTILKEPERLGEANKRALLPKGGAMLWQYRIRNLFLLSFMVTLI
                     LAGIESTFQLFQISAIEITPKQIGYLFMASGFVDAAIQGGVVRRVKNGSETKWILGGQ
                     IVTAIGLLLIPFTGNLFWAGVALSVFTAGNALSRTVLVSLTSKESGGKYGTAAGMTYS
                     MDNMGRIIGPLLFTWLFTRMTGEIYFISAALAIISIVFIFTYRMSSKTLRSA"
     gene            complement(3533..4885)
                     /locus_tag="VFX34_09495"
     CDS             complement(3533..4885)
                     /locus_tag="VFX34_09495"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5565185.1"
                     /translation="MSIEQLKEQLFTEGEKMGFADLELYYEKKESLTIGLFEGEVDEY
                     DFSDVQGAAVRGLFDGKTGYAYTESFDEDSVSFLLKNAAENAGLIENEPEELFTEKTQ
                     YEEVQFYSPELDAVKPEDMIQFLQEVEKKILSYDQRVTKITASQMRTQKSEKGLYHDK
                     GLELKEANNFLLFYVSILVQENSELKSGFVFRITKDFNELDAEKVAKEVVEQGLSKLG
                     ERNYPNKKYPVVLKNDAAASLLSTFTSSFSAQAVQDNQSRLKGKLGEKIAAHHVNLID
                     NPFLPAGIQSATFDSEGVPTRKLNIVEDGNLQTYFHNLKTAKKDGVETTGHGHRSSYQ
                     GSVEVSPSNFYIEPANQTYEQLYAGIEEGIIITDLAGLHSGADPISGDFSLAADGYFV
                     KDGNIVGATKQMTVAGNFFEVLQDIEEIGSDLQFSPFSFSGYIGSPSLKVKGLAITID
                     "
     gene            complement(4889..>5739)
                     /locus_tag="VFX34_09500"
     CDS             complement(4889..>5739)
                     /locus_tag="VFX34_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX5565186.1"
                     /translation="GADMQTGFYGPGAYQGFEFIENLDLNHYAKESARIAKTMLHADP
                     APSGKFPVIIDNEFGGVIFHEACGHSLEATAVAKNASVFANKVGEKVASDLVTYIDDG
                     TLENEWGSLNIDDEGEKSRKNVLIEDGILKGYMIDKLNGRRMGMESTGSSRRQSYRFA
                     PTSRMTNTYIAPGKSTPEEIIADTEYGIYAKYMGGGSVNPGTGDFNFAVNEAYLVKNG
                     KIERPIRGATLIGNGAKTILDIDRVGNNLGHGAGMCGASSGSLPVNVGQPMVRVSEIT
                     VGGKEA"
BASE COUNT         1390 a         1446 c         1249 g         1654 t
ORIGIN      
        1 atgaaaggtc ctatacctgt atttgtatgc gaagatggtc aatagtttaa tattgtcata
       61 tataaaattc cgtacaaaca cttcattttc cgctataatg aaatgtggtt tccacaccaa
      121 tccaataact gataaggtga attatatgaa tatagataaa aaacctgtgg accgtttcga
      181 ttggccactt gcattcatcc ttcttctgtt tgcgacaatc agcatcatcg ccatcgcatc
      241 cgcccagacg acaggacaat ataaaataga ttccataccg gtaaacttca tcccgtcaca
      301 aatccaatgg tacgccatcg ggtctattat catcgccctt acgatgtatc tggagccgga
      361 acaatacaag aaagccgcct ggatcatcta tggcggtggt gtgttcctcc tggtcatgct
      421 tttcattttg cccgaaggca tggaacttgt ctcccgcaga aatggtgcta aaagctggtt
      481 ccacctgcca ggcatcggaa gcatccagcc cgccgaattc atgaagacat tcttcatcat
      541 cggcatggcc cgcacaatca cgaagcatca cgaacgctat ttggaaaaga cgttgcagac
      601 ggatttcctg ctgctcggta aaatcgctgc tgtcttcctc cttccgttct cgttcatcat
      661 gcttcagccc gatttaggaa ccgggctcgt cttcatcgcg atcaccgtcg ccttgatcat
      721 tgtggcgggc atcacttggc ggatcatcct tccgattttc ctcggaacgg ccgccttggg
      781 aggaacgctt ctttggatgg cactttatgc gcaggaattt ttggcgaagt tcgggtttaa
      841 gccgtatcac ttcgaacgga tctattcgtg gctcgatccg tactcctatg cgtcgaatga
      901 agggcggcac ctcatcacct cgttgaacgc catcggctcg ggggaaattt tcgggaaagg
      961 cttcaaagga cgcgaagtgt atgtcgcgga aagccataca gacttcattt tcagcgtcat
     1021 cggcgaggac tggggattca tcggcgcaag cctcgtcatc tgtctctact tcttcctgat
     1081 ctatcatatg acgaagatca cgctcgaatt gaaggatcca ttcagcacct acatttgtgc
     1141 aggcatcatc gccatgatca cattccacgt gttcgaaaac atcggcatga cgacccagct
     1201 actgccgatt acaggcatcc ccctcccgtt catcagttac ggaggcagtt cattgatggg
     1261 caatatgctg gcaatcggcc ttgtcttcag catgaagttt catcacagaa cgtatatgtt
     1321 cagttcggat gatgaggaat aacgaaaagc ggaaacgacc gattaggtcc gacaggcaaa
     1381 gccgtaacga agaacggctt ttgcgagtaa aagcgaagcg ttaggagcaa cggttacctc
     1441 tacacagcaa ctttaggtta tgacccgagg acctggtcgt tggagctgga caataacgaa
     1501 aagcggaggg cgcccgctgc agaaacgcat aaattgtttt attcgcgcat aaacggctgt
     1561 aaaaacgcat aaatctattt tcccacgcat aaactgcccg aaatgctcat aaaccttttt
     1621 attcacgcat aaactatcgt aaacgcgcat aaaccttttt attaacgcat aacgcacagt
     1681 aaacgctcat aaaaaaatct cccgcatgca agtatgcggg agtttttcat tatgactgta
     1741 tttgcgggtc atttgcaggt ggcgcgaggg ctttcagcat ttccatacgg gacttcgttg
     1801 ctgttgccga aacacccaac ttcgccaagt tggagatgat tttttttaaa tcaacttttt
     1861 ctttcgccat cttttccacc tcgacctttc tatatgttgt tgacgtatca tccattggaa
     1921 agtttcatta ttgaaatgaa aatcatgcca attttcttct atacacatca taccactcct
     1981 gtccactttt aaatctttca attctgttac aaattgttcg ccttttgtaa atgctttata
     2041 catgaaggaa aataaaagat tggaatgggg gtcatttatg cgtgagtttc ggaatttatg
     2101 cgcgctttcg ccgatttatg cacgtgcaga gggatttatg cgtggctatc ttgatttatg
     2161 cgcgagtgca gatttttatg cggcattaaa tggatttatg cgtttttgga aagggttttg
     2221 aggatattgg cagctcttaa catacatatt ctttgtaact gcaagtgcag cgaagtaaaa
     2281 tggtcatatt gatgtttact gaaagcaggt acatacatga aaaaatcaat tttactatta
     2341 atgtccgtac aatttttcgt ctatctcggt tttggcatca tcattcccgt tttaccggaa
     2401 gtgattgtgc agcaggatct ttcggaaatt catgttggcg gattgctgac ggtctattcg
     2461 ctcgcatcct tcttcaccgc cccgctttgg ggttcattat ccgatcggac gggacggaag
     2521 aaactgatca tcatcggtct catcggcttc agcctaagct ttttcctgtt cgccttcttc
     2581 attcacaacc tgacgttgct ttatctttcg cgtgtcgtcg gaggaatttt ctccggggca
     2641 ttgtacacgg cggtgaccgg atttgtcggg gatatgacga cggaggaaaa tcgcaataaa
     2701 tatatgggct tgctcggcat gtcgatcggg ctcgggttca ttttcgggcc tgcaatcggc
     2761 ggcgtactcg gccattacag cttacagttg ccgttcatcg catcaggcgt attgacgttg
     2821 ttactagtca tctacgctag cactatcctt aaagaacctg aacgtctcgg cgaagcgaat
     2881 aaacgggcat tgctcccgaa aggcggcgca atgctttggc aataccggat ccggaatttg
     2941 ttcctgctct ccttcatggt gacgttgatc ctggcaggaa tcgaatccac attccagttg
     3001 ttccagattt ccgctatcga gatcacacct aagcagatcg gctatttatt catggccagc
     3061 ggatttgtcg acgcggccat tcagggcggt gtagtgcgac gcgtaaaaaa cggttcggaa
     3121 acgaagtgga ttctgggcgg tcaaatcgtt accgctatag gactcctttt gattccgttc
     3181 acgggcaatc ttttctgggc gggcgttgca ctcagcgtct tcaccgcagg aaacgcactt
     3241 tcgcggacag tccttgtttc attgacatcg aaggagtcag gcgggaaata cggaactgca
     3301 gccggcatga cgtattcgat ggacaatatg gggcgcatca tcgggccgct tctattcaca
     3361 tggctcttca caaggatgac gggcgaaatc tatttcattt ccgcagctct tgcgatcatt
     3421 tcaatcgtct tcattttcac gtatcgaatg tcttcgaaga cgttaaggtc cgcataaaaa
     3481 taaaggcaca gcatatgcat cgcgcatata tgctgtgcct tttcttattt acttaatcaa
     3541 tcgtaatggc caagcccttt actttcaggg aaggagaacc gatataccca ctgaaactga
     3601 acggtgagaa ttgcaggtca ctgccgatct cctcaatatc ttggagcact tcaaaaaagt
     3661 ttcctgctac agtcatctgc ttcgtcgcac cgacaatgtt gccgtccttt acaaagtaac
     3721 cgtctgccgc caatgagaag tcgcctgaaa tcggatccgc tccggagtgc agtccagcta
     3781 aatcagtaat gatgatgcct tcctcaatcc ccgcgtagag ctgctcgtac gtttggttcg
     3841 caggctcgat atagaaattg gacgggctca cttcaacaga gccttgatag gaactgcgat
     3901 gtccatggcc tgtagtctct acaccatctt ttttcgccgt tttcaagtta tggaaatagg
     3961 tttgtaagtt cccatcctct acgatgttaa gctttcgcgt cggaaccccc tccgaatcga
     4021 atgtcgcact ttgaatcccg gctggaagaa atggattatc aatgagattc acatgatgcg
     4081 ccgcaatctt ctcgcctaat ttccctttca gacgggactg gttatcctgt acagcctgtg
     4141 ccgagaaaga ggacgtgaat gttgacaaaa gggaagccgc tgcgtcattt ttcaatacga
     4201 cagggtattt tttattcgga taattccttt cgcccaactt gcttaagccc tgttctacga
     4261 cctcttttgc aactttctct gcatccagtt cattaaaatc cttcgtgatc ctaaaaacaa
     4321 agccggattt caattcgcta ttttcctgga cgaggattga gacgtaaaaa agtaaaaagt
     4381 tattcgcttc cttaagctcc aaccctttgt catggtaaag gcccttttcc gatttctgtg
     4441 tccgcatttg ggatgcggtg atcttcgtta cccgctggtc atacgatagg attttcttct
     4501 ccacttcctg taggaattgg atcatatcct ccggcttgac tgcatccagt tccggactgt
     4561 agaactgaac ttcttcatac tgcgtttttt cagtgaataa ttcttccggt tcattttcaa
     4621 tcaatcccgc attctctgcc gcgtttttca gcaagaatga aaccgagtcc tcatcgaaac
     4681 tttccgtata ggcatagcct gtttttccat caaacagccc tcgtaccgct gcaccttgaa
     4741 catcggaaaa gtcgtattca tccacttccc cttcgaatag cccaatggtg aggctctctt
     4801 ttttctcata ataaagttcg agatccgcga aacccatctt ttccccttca gtaaacagct
     4861 gctctttcaa ttgttcaatg ctcatcgttt acgcctcctt tccgccgact gtaatttcac
     4921 tgacacgaac catcggctgc ccgacattca ctggcaaact gccacttgat gcgccgcaca
     4981 ttccagcacc gtggcctaaa ttattgccga cgcgatcgat atccagaatc gttttcgcac
     5041 cgtttccaat caacgtcgcc ccacggatcg gcctttcaat ttttccgttc ttgaccaagt
     5101 acgcttcatt tacggcgaag ttgaaatccc ccgttccagg attgaccgaa ccgccgccca
     5161 tgtacttcgc gtaaatccca tattccgtat ccgctatgat ttcttcaggt gtggattttc
     5221 caggagcgat atacgtattc gtcatccgtg atgtcggagc aaatcggtac gattgcctgc
     5281 ggcttgaacc tgttgactcc attcccatcc gtcttccgtt cagtttatca atcatatatc
     5341 ctttcagaat gccgtcttca ataagcacat ttttacggga tttttcgcct tcgtcatcga
     5401 tattgagcga accccactca ttttcaagcg tcccgtcatc gatgtatgta actagatctg
     5461 acgccacttt ttctcctact ttattcgcga atacggaagc atttttcgcc accgctgtcg
     5521 cttccaggct gtgtccgcaa gcttcgtgga aaatgacgcc gccgaattca ttgtcgatga
     5581 tgacagggaa cttaccgctc ggcgccggat ccgcatggag catcgtcttg gcaatccggg
     5641 cagattcctt cgcataatgg ttgagatcga ggttttcaat gaactcaaaa ccttggtatg
     5701 ccccaggacc atagaagccc gtttgcatat ccgcgccat
//
LOCUS       DASULI010000437         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1435401,
            whole genome shotgun sequence.
ACCESSION   DASULI010000437 DASULI010000000
VERSION     DASULI010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435401"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFX34_09505"
     CDS             complement(<1..189)
                     /locus_tag="VFX34_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5565187.1"
                     /translation="MIHVQELTHSFKIGKKGKERQVPVLKGLSFKINEGEIVSIVGRS
                     GSGKSTLLHILAGFLKPDA"
     gene            complement(395..1945)
                     /locus_tag="VFX34_09510"
     CDS             complement(395..1945)
                     /locus_tag="VFX34_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEX5565188.1"
                     /translation="MPGISGMFIVIGIVVFILLAVILVYVSKYKTVGPDEALIVTGSY
                     LGSKNVHTDDSGNRIKIIRGGGTFVFPVFQQSEPLSLLSSKLEVTTPEVYTEQGVPVM
                     ADGTAIIKIGGSISEIATAAEQFLGKSKADRENEAKEVLEGHLRSILGSMTVEEIYKN
                     RDKFSQEVQRVASQDLAKMGLVIVSFTIKDVRDKNGYLDSLGKPRIAQVKRDADIATV
                     EAEKETRIKNAEASKEAQKAEIERATEIAEAEKENQLKVADYRREQDIAKARADQAYE
                     LESARAKQEVTEQEMQVRIIERQKQIELEEKEILRREKQYDSEVKKKADADRYAIEQN
                     AAAEKSRQMAEADAEKYRIEARAAADAEKIRLDGLAKADSQRAQGESEADVIRLKGLA
                     EAEAKRKIAEAFEQYGQAAVLDMIVKMIPEYAKQVASPLSNIDKITVVDTGGGEGGGA
                     NKVTSYATNLMSTLQESLKASSGIDVKEMMESYVGKNNLRPSLDNLAEEMRSAKPVAV
                     KPKPVDEE"
     gene            complement(1981..>2398)
                     /locus_tag="VFX34_09515"
     CDS             complement(1981..>2398)
                     /locus_tag="VFX34_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565189.1"
                     /translation="SPILDPAVILAFISFTSAVGYILELVTEWNHLLILIIALGAATA
                     LDFLLYFFVLLPLKSAEVSLAYTDESLTGQVGKVIVPIPVDGFGEIIIESVNGIISKR
                     AAGYENKAIDYGKEVLIIEVKEGTFIVKEYEPFRFK"
BASE COUNT          516 a          631 c          534 g          717 t
ORIGIN      
        1 ggcatccggt ttgaggaacc ctgccagtat atgtagcagt gtcgacttcc cggatccact
       61 tcggccgaca atcgatacga tttctccttc attgattttg aacgaaagcc cttttagcac
      121 cggcacttgc cgctccttgc ctttctttcc gattttgaat gaatgtgtca attcctgaac
      181 gtgtatcata tattcccctc caacgtatta actgtttttc attgtagggg gagaatctta
      241 agggagagtg aggagaaaaa tgaagaaatt cttaagatgt gggaaggctt tggacgcgga
      301 gcaattttta gctgaaatat ctttggtcgc gcaaaatgcc tttgccaaca aaaatacacc
      361 cgaaccacaa atgtggttcg ggtgcaactg tttcttattc ctcatcaacc ggcttcggtt
      421 taacagcaac cggtttggct gagcgcattt cttccgccaa gttgtcgagg cttggtctta
      481 agttgttctt tccgacgtag ctttccatca tttcttttac gtcaatgcca gatgaggctt
      541 tcaatgattc ttgcaatgta gacattagat tcgttgcgta cgatgtcact ttattggcac
      601 cgccaccttc accgccgccc gtgtcaacga ctgtgatctt atcgatgttc gaaagcgggc
      661 ttgcgacttg cttcgcatat tcagggatca tcttcacaat catgtcaagc acagccgcct
      721 ggccgtattg ttcgaatgct tccgcgattt tacgcttcgc ttcggcttcc gcaagacctt
      781 tcaggcggat gacgtctgct tccgattcac cttgcgcgcg ttgcgagtcg gcttttgcca
      841 atccgtcgag tcggattttc tctgcatcgg cggcagcacg tgcttcgatg cggtatttct
      901 ctgcatccgc ttccgccatt tgacgggatt tttcagcggc tgcgttttgc tcgatcgcat
      961 agcggtccgc gtcggccttt ttcttaactt cggaatcgta ttgcttctca cggcgcagaa
     1021 tctctttctc ttccaattcg atctgctttt gacgctcgat gatgcgaacc tgcatctcct
     1081 gctccgtaac ttcctgcttc gctcgtgcgg attcaagctc atacgcttgg tcggcgcgtg
     1141 ctttcgcgat atcttgctca cggcggtagt cagcgacttt cagctgattt tccttctcgg
     1201 cttcagcaat ttcagttgca cgttcgattt cagctttttg cgcttccttc gatgcttcag
     1261 cattcttgat gcgcgtttct ttctcagctt caaccgtcgc gatgtcagca tcgcgcttca
     1321 cttgggcgat acgcggttta ccgagtgaat ccaagtagcc gtttttatca cgaacgtctt
     1381 tgattgtgaa ggaaacgatg acaagcccca ttttagccaa gtcctgcgac gcgacacgtt
     1441 gcacttcctg ggagaactta tcacggttct tatagatctc ttcgaccgtc atggacccta
     1501 gaatggaacg cagatgtcct tccagcactt ccttcgcttc attttcgcgg tcggctttcg
     1561 attttcctaa aaactgctcc gctgccgtcg cgatttcaga aatcgatcct ccgatcttaa
     1621 tgatggcagt tccgtctgcc atgaccggaa caccttgttc cgtgtacact tcaggcgtcg
     1681 tcacttccaa tttgcttgaa agtaagctga gcggttccga ttgttggaac actgggaaga
     1741 cgaatgttcc cccgccacgg atgattttga tccggttgcc cgagtcatcc gtatgtacgt
     1801 tttttgatcc taaatagctt cctgtcacga ttagcgcttc gtccggcccg actgtcttgt
     1861 acttcgaaac gtacacgagg atgacagcta gcaaaatgaa tacgacaatc ccaatgacaa
     1921 taaacattcc tgaaattcct ggcattctga ttccccctta atgattttgt ttcgtcttgc
     1981 tcatttgaag cggaaaggct catactcctt tacgatgaac gtcccttcct tcacttcgat
     2041 gatcagcact tcttttccgt agtcgatcgc tttgttttcg tacccggcag ccctttttga
     2101 aatgatacca ttcaccgatt cgatgatgat ttcgccgaag ccgtcaaccg gaatcggaac
     2161 gatgactttg ccgacctggc ccgtgagtga ttcatccgta taggcaagcg atacttccgc
     2221 ggatttcaat ggaaggagca cgaagaaata tagaaggaag tccaatgcgg tcgctgcccc
     2281 aagcgcaatg atcaagatca gcaaatgatt ccattctgtc actaactcca aaatataccc
     2341 gactgctgat gtaaatgaaa tgaatgcaag aatgactgcg ggatccaaaa tcggactc
//
LOCUS       DASULI010000438         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_597582, whole
            genome shotgun sequence.
ACCESSION   DASULI010000438 DASULI010000000
VERSION     DASULI010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597582"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /gene="glyA"
                     /locus_tag="VFX34_09520"
     CDS             complement(<1..1226)
                     /gene="glyA"
                     /locus_tag="VFX34_09520"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5565190.1"
                     /translation="MELANVKREDQAVYEAIMAEKKRQQANIELIASENFVSQAVMEA
                     QGSVLTNKYAEGYPGKRYYGGCEHVDVVENIARDRLKEIFGAEYANVQPHSGAQANMA
                     VYFTILETGDTVLGMNLSHGGHLTHGSPVNFSGIQYNFVEYGVSKDDEKIDYEDVRQK
                     ALEHKPKLIVAGASAYPREIDFAKFREIADEVGAYFMVDMAHIAGLVAAGEHPNPVPH
                     AHFVTSTTHKTLRGPRGGLILCKEEFGKKIDKSIFPGIQGGPLMHVIAAKAVAFGEAQ
                     KPEFKDYIKQVKANAKVLGESLVSEGIDIVSGGTDNHLLLVNLRSLGITGKIAEHVLD
                     EVGITVNKNTIPFDTESPFVTSGIRIGTPAVTSRGFKEEEMKEIASIMAMLLKNHEDE
                     NVKKEAKERVAALTGK"
     gene            complement(1231..1794)
                     /locus_tag="VFX34_09525"
     CDS             complement(1231..1794)
                     /locus_tag="VFX34_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEX5565191.1"
                     /translation="MDALQLWKLQLEQLLEEMEEQIELEPDSIFVVGCSTSEVAGKRI
                     GTAGALEIAEAFFKPLQSFAERNQLHLAFQGCEHINRALTVEKKTARTFQLEPVTVIP
                     DVSAGGSMSAYAFRQMKQPVVVEEIRANAGIDIGQTLIGMHLKRVAVPVRTSISEIGD
                     AIVTAATTRPKLIGGERAIYKLQEEGE"
     gene            complement(1815..2252)
                     /gene="rpiB"
                     /locus_tag="VFX34_09530"
     CDS             complement(1815..2252)
                     /gene="rpiB"
                     /locus_tag="VFX34_09530"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX5565192.1"
                     /translation="MKIAISSDHGGNNLRREIISLLTELGIEYEDFGPDSDESVDYPD
                     YATPVADGVAKGNFDRGILICGTGIGMSIAANKVRGIRCALVHDVFSAKATRGHNDSN
                     VLAMGERVIGPGLAREIVETWLKAPFEGGRHERRIGKLMDLEG"
     gene            complement(2436..>2968)
                     /locus_tag="VFX34_09535"
     CDS             complement(2436..>2968)
                     /locus_tag="VFX34_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX5565193.1"
                     /translation="AGQTNLLALNASIEAARAGEHGKGFAVVAEEVRILADESAQAVK
                     GISQLVQTIQSDVSKVVVEIEEQVQTASLEADRANETSGNMESMADTVNGMADSVVEI
                     TKFIENQLTNIETTATQSQDVAAIAEETSAAALEVGSATGEQARSIEQVNEMAVELKK
                     QSEQLYSVISRFDLTK"
BASE COUNT          676 a          810 c          618 g          864 t
ORIGIN      
        1 aacttgcctg tcaatgcagc aacgcgttcc ttcgcttcct tcttcacgtt ctcatcttca
       61 tggtttttca aaagcattgc catgatcgat gcgatttcct tcatttcctc ctctttgaag
      121 ccgcgggagg tgaccgcagg agtaccgatg cggattccgg acgtgacgaa tgggctttcc
      181 gtgtcaaacg gaattgtgtt tttgttcaca gtgatgccga cttcgtcaag gacgtgttcc
      241 gcaattttcc ctgtgatgcc aagggagcgg aggttcacaa gtagaaggtg gttgtctgtt
      301 ccgccggaaa cgatgtcaat gccttccgaa accaacgact cccctaggac tttcgcgttt
      361 gcttttactt gtttgatata atctttgaac tcaggctttt gcgcttcacc gaatgcaact
      421 gctttcgccg cgatgacgtg cattaaagga ccaccttgaa ttccagggaa gatggattta
      481 tcgattttct taccgaactc ttctttacat agaatgagtc ctccgcgagg tccacgcaat
      541 gttttatgcg ttgtcgacgt gacaaagtgt gcatgcggta ctgggtttgg atgttcgcct
      601 gctgcgacca gacccgcgat gtgcgccata tcgaccataa agtacgcgcc gacttcatcc
      661 gcgatttcac ggaatttcgc aaagtcgatt tcacgtggat acgcacttgc acccgcaaca
      721 atcaattttg gcttgtgctc aagcgctttt tgacgaacgt cttcgtaatc aatcttttcg
      781 tcgtctttgc tcacgccgta ttctacgaaa ttgtattgaa tgccagagaa gttgaccggg
      841 ctgccgtgtg tcaagtgacc gccatgggac aagttcatcc ctagcacggt gtcgcccgtc
      901 tcaagaattg tgaaataaac cgccatgttc gcttgtgcac cggagtgtgg ttgcacgttt
      961 gcatattccg caccgaaaat ttctttcaga cggtcgcgcg caatattttc aacaacatcc
     1021 acgtgttcac agccaccata ataacgcttt ccaggatagc cttccgcata tttattcgtc
     1081 agtacagagc cttgtgcttc cataacagct tgggaaacaa agttctctga cgcgattaat
     1141 tcgatgtttg cttgctgacg ctttttctcc gccatgatcg cttcatatac agcttgatcc
     1201 tcacgtttta cgtttgccaa ttccatgatc tcattcccct tcttcttgta gtttatatat
     1261 tgcacgctct ccgccgatta gtttaggtct cgtcgtagca gccgtaacaa tcgcatcacc
     1321 aatttcgctg attgacgtcc gaaccgggac cgctaccctc ttcaagtgca ttccgatcaa
     1381 tgtttgtccg atgtcaattc ccgcatttgc gcgaatttcc tccacaacga ccggttgctt
     1441 catctggcga aatgcatacg cactcattga accacctgcg gatacatccg gaatgactgt
     1501 gaccggctcc aactggaatg tacgagccgt cttcttttca accgtcagtg cacgatttat
     1561 atgttcacag ccttgaaacg caagatgaag ttgattgcgt tccgcgaacg attggagcgg
     1621 tttgaagaac gcctcggcaa tttcaagcgc tcctgccgtt ccaattcgtt tcccggccac
     1681 ttcagaggtc gaacaaccta caacaaatat gctgtccggt tccaactcaa tctgctcttc
     1741 catttcctcc agaagctgct ccagttgaag cttccacaac tgcaaagcat ccattctgtc
     1801 gccctcctgt ctgttcagcc ttctaaatcc atcaattttc caattcgacg ctcatggcgt
     1861 ccgccttcaa acggcgcttt cagccacgtt tctacgattt ctcttgcaag accaggaccg
     1921 atgacacgct cccccattgc aagtacgttt gagtcattat gtccccgtgt cgcctttgca
     1981 ctaaatacat catgcacaag cgcacagcga atgcctctca ctttgtttgc ggcaatcgac
     2041 ataccaattc cggttccgca aatgaggatg cctcggtcaa agttcccttt tgcaaccccg
     2101 tctgctacag gtgttgcgta gtcagggtag tcaacagact catccgaatc tggaccaaaa
     2161 tcttcatact caattcctag ttcagttagc aatgatatga tttcacggcg aaggttgttg
     2221 ccgccatgat cggaagaaat cgcgattttc atcgtgtccc tcttttccta atttacttag
     2281 tattcattct accatttttc aacgacaaaa aatacagccc tgcgtttgca gagctgaaaa
     2341 agttattcta tgaatagtga ttctccatcg ggttttctcc aaaaagcttg atctattgac
     2401 gttatgcgcg aataaaacca atggaaaaaa ccaaattatt tcgttaaatc aaaccggcta
     2461 atgacactgt atagctgttc agactgcttc tttaattcaa ctgccatctc gttaacttgt
     2521 tcaatggatc tcgcttgttc tccggttgca gacccgacct ccagcgcagc agcagacgtc
     2581 tcctctgcaa ttgccgcgac gtcttgggat tgcgttgcag tcgtttcaat gttcgttaat
     2641 tgattctcaa taaacttcgt aatttccaca acggagtccg ccatgccatt cactgtatct
     2701 gccatagact ccatgttgcc gctcgtttcg tttgcacgat ccgcttccaa ggaagccgtt
     2761 tggacctgct cctcgatttc aacgacgacc tttgatacgt ccgattgaat cgtttggaca
     2821 agctgggaaa ttccctttac cgcctgcgca ctttcatccg cgagaatccg gacttcctcc
     2881 gcaacgacgg caaatccttt tccatgctcc ccggcgcgag ctgcttcaat ggatgcattt
     2941 agggcaagca aatttgtctg cccggcaa
//
LOCUS       DASULI010000439         4378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1084191,
            whole genome shotgun sequence.
ACCESSION   DASULI010000439 DASULI010000000
VERSION     DASULI010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084191"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="hisD"
                     /locus_tag="VFX34_09540"
     CDS             <1..712
                     /gene="hisD"
                     /locus_tag="VFX34_09540"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5565194.1"
                     /translation="AYGTETVRKVMKITGPGNAYVARAKKWVFGDVGIDMIAGPSEIC
                     VLADETANPAFAASDLLSQAEHDERAAAICVTTSELFAGKLVAEVEMRLSDMDRREIA
                     SRSIKDFGRIIVVDSLEEAIEVVNKIAPEHLQIMMDHPMATLPYIRNAGAIFIGEFSP
                     EALGDYMAGPNHTLPTSGTAAFSSPLGVYDFVKKSSIIHYTELALEEVAGDIIVLAEA
                     ERLAGHAESIRMRKGKAE"
     gene            709..1293
                     /gene="hisB"
                     /locus_tag="VFX34_09545"
     CDS             709..1293
                     /gene="hisB"
                     /locus_tag="VFX34_09545"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX5565195.1"
                     /translation="MRSSRISRSTSETAIELEFSIDGTGKTDIRTGVGFLDHMLTLFA
                     NHGRFDLTVACEGDLDVDQHHTVEDIGIALGQAFNRSIGKKEGIERYSTVSTPMDEAL
                     STVSLDISGRSFLVYNIEGLKDKVGAFDTELVEEFFLAFTRHANVTLHINLQYGKNSH
                     HIIESIFKSFGRALRIASAINPEIEGVPSTKGIL"
     gene            1307..2053
                     /gene="hisA"
                     /locus_tag="VFX34_09550"
     CDS             1307..2053
                     /gene="hisA"
                     /locus_tag="VFX34_09550"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX5565196.1"
                     /translation="MILFPAIDIRGGKCVRLIQGDYDRETVYNDSPSDMAKEWEKQGA
                     EYIHVVDLDGAKTGDSLNKKAIRQITEAVQIPVQVGGGVRSMETIDSLIAIGVSRVIL
                     GTAAIQDPRFVEEAVAKYAGKIAVSIDARNGFVATDGWTETTDVKAVKLLRWLEGIGV
                     KTVVYTDIQKDGMLQGPNFDELYVINKASEMEIIASGGVSTYLDIVKLNMMNLYGAII
                     GKALYEGNLSLETALEAIRDEETNYTLHGR"
     gene            2019..2780
                     /gene="hisF"
                     /locus_tag="VFX34_09555"
     CDS             2019..2780
                     /gene="hisF"
                     /locus_tag="VFX34_09555"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017187686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX5565197.1"
                     /translation="MKKRIIPCMDVDNRKVVKGKKFLDVQEVADPLELAKKYVEDGAD
                     ELVFYDISASTKNRDMFLDLIEEIAKEVPIPFIVGGGIRTIEDIKKILSIGGDKVSIN
                     SAALLNPGLIREAAEQFGSERIMLSMDVKRTGPGKWTVFAKGGMEDTGVDAIEWAIKG
                     EQMGAGELVVNSIDEDGVKDGYDIELNKAMAEAVSIPIVASGGAGKPEHFAEVLSEGK
                     ASAALAASVFHFDEIQIGELKTYLEAQKQVVGNES"
     gene            2777..3415
                     /gene="hisIE"
                     /locus_tag="VFX34_09560"
     CDS             2777..3415
                     /gene="hisIE"
                     /locus_tag="VFX34_09560"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEX5565198.1"
                     /translation="MMNIDELVFDQNGLIPAIVQDERTGEVLMLAYMNQESVRKTIET
                     KETWFYSRSRQELWNKVATSGNRQIVKRISFDCDQDAILLQVKPVGPACHTGETTCFY
                     TSDFEEGNSVRGVVHEVVDEIKERRANPVEGSYTSYLFREGVDKVLKKIGEESTEVVI
                     GAKNRDKDELTNELADLTYHTLVLMELLDVSVDDVKAILRDRRPHKEVVPNE"
     gene            3408..>4378
                     /locus_tag="VFX34_09565"
     CDS             3408..>4378
                     /locus_tag="VFX34_09565"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5565199.1"
                     /translation="MNKYWSEIVKRTEPYVPGEQLNDPGIIKLNTNENPYPPSPLVKE
                     AILEELDGQLRLYPSPTADGLRETIAESVGVSKEEVFVGNGSDEVLAFSFMAFFQPGK
                     PIRFPNITYSFYPVYAKLFDIPYELVRLNEDFSLPVESFFNSEGGVILPNPNAPTSLY
                     MELSSIEEILIGNPDTVVIIDEAYIDFASESAVELVKSYGNLLVVQTTSKSRSLAGMR
                     VGYAIGHPDLIEAVVRIKDSFNSYTIDRLAIAGATAAFKDGAYFDKTIMKIKETRSRF
                     IEAMKGMGFHVLPSQANFVFVSHSDMAASDLYRQLKEEGILVRHFNQP"
BASE COUNT         1202 a          918 c         1222 g         1036 t
ORIGIN      
        1 tgcctatggc acggaaactg tccggaaggt gatgaaaata actggacctg gcaatgcata
       61 tgttgctagg gcgaaaaaat gggtgtttgg cgacgttgga atcgacatga ttgcaggtcc
      121 aagcgaaatt tgcgtgttgg cggatgagac tgcgaatcct gcatttgctg cttccgatct
      181 tctatcccaa gcggagcatg acgagcgcgc agctgccata tgtgtgacga caagcgagct
      241 gtttgccgga aagcttgttg cggaagttga aatgcggtta tccgacatgg atcgtcggga
      301 gattgcaagc cgttccataa aggatttcgg caggataatc gtcgttgatt cgctggagga
      361 agcaatcgaa gtggtaaaca aaattgcacc ggagcatttg caaatcatga tggatcatcc
      421 gatggcaaca ttgccttata tccggaacgc aggcgctatt ttcattggag agttctcgcc
      481 ggaagcgtta ggcgattata tggcgggacc gaatcataca ttgccgacga gtggaacagc
      541 cgctttttcg tctccgttag gcgtttatga cttcgtgaaa aaatcgagca tcatccatta
      601 cacggaattg gctttggagg aagtggcggg cgacatcatt gtattggcgg aagcggagcg
      661 gctggcgggc catgccgaat cgatccggat gaggaagggg aaagcggaat gaggagttca
      721 aggatttcga gaagcacttc ggaaacggcg attgagcttg agttttcgat cgatggaaca
      781 ggcaaaacgg atatacggac aggtgtcggt tttctcgacc atatgctcac gttgttcgcg
      841 aatcatggca gattcgattt gacggttgcg tgtgaaggcg atcttgacgt cgatcagcac
      901 catactgtag aggatatcgg aatcgcgctc gggcaggcgt tcaatcgcag cattggaaaa
      961 aaagaaggca ttgaacggta ttcaacggtg tctacgccga tggatgaagc actatcgacg
     1021 gtgtcactcg acattagcgg acggtccttt ctcgtttata acattgaagg actgaaagac
     1081 aaagtagggg catttgatac cgaattggtg gaggagtttt tcctcgcttt tacgagacat
     1141 gcgaatgtga cgttgcatat caatttgcaa tacggaaaaa acagccacca tatcatcgaa
     1201 tccattttca aaagttttgg tagggcattg cggattgcaa gtgccattaa tccggaaatt
     1261 gaaggggttc catcaacaaa gggcatttta taagggggaa gcgtcaatga ttttatttcc
     1321 ggcaatcgat attagaggag gaaagtgcgt ccgtctcata caaggagatt acgaccggga
     1381 gactgtgtat aatgattcgc cctccgatat ggcgaaggaa tgggaaaagc aaggcgccga
     1441 gtacatccat gtcgtcgact tggatggggc gaagactggt gattcattga acaaaaaagc
     1501 gattagacaa attacggagg ccgtgcaaat tcccgtgcaa gtcggcggtg gagtgcggtc
     1561 aatggaaacg atcgattcac tgattgctat cggcgtcagc agggtcattc ttgggacggc
     1621 agccattcag gatccccggt tcgtcgaaga agcagtcgcc aaatatgccg ggaaaatcgc
     1681 agtgtcgatc gacgcccgaa atggattcgt tgcgacggac ggctggacgg aaacgacgga
     1741 tgtcaaagcg gtcaagttgt tgcggtggct cgaggggatc ggcgtcaaaa cggtcgtcta
     1801 taccgacatt caaaaagacg gcatgctgca agggccgaat ttcgatgaat tatatgtcat
     1861 caacaaagcg tccgaaatgg agatcatcgc ttccggcggt gtttcgacgt acctggatat
     1921 cgtcaagttg aatatgatga atctgtatgg cgccattatc gggaaggcat tgtatgaagg
     1981 gaatctatcc ttggaaacag cattggaggc gatcagagat gaagaaacga attatacctt
     2041 gcatggacgt tgataaccgg aaagtggtga aagggaagaa gttcctggat gtccaggaag
     2101 ttgcggaccc gcttgaactc gcgaaaaaat acgtggagga tggcgcagat gagctggtct
     2161 tctatgatat ttccgcctct acgaaaaaca gggacatgtt cctcgatttg attgaagaga
     2221 ttgcaaagga ggtcccgatc cctttcatcg tcggtggagg cattcggaca attgaagaca
     2281 tcaagaaaat ccttagcatc ggcggggata aagtgtccat caatagcgcc gctttgttga
     2341 atccagggct catccgggaa gctgcggagc aattcggctc cgaacggatt atgctctcga
     2401 tggacgtcaa gcggaccggc cccgggaaat ggactgtctt tgcaaaaggc ggtatggaag
     2461 atacgggagt ggatgcgatc gagtgggcga ttaaaggcga gcagatgggt gccggggaac
     2521 tggttgtcaa cagcatcgat gaagatggtg tgaaagatgg ctatgatatc gagctcaaca
     2581 aagcgatggc cgaagcggtg agcataccga ttgttgcaag tggaggagcg ggcaagcccg
     2641 aacatttcgc ggaagtgttg agtgaaggga aagcgagcgc tgcccttgcc gcctcggtat
     2701 tccactttga tgaaatccaa atcggtgaat tgaagaccta tttggaagca caaaaacaag
     2761 ttgttgggaa tgaatcatga tgaatatcga tgaacttgta tttgatcaga acggtttgat
     2821 tcctgctatc gtgcaggacg agcgtacagg cgaggtgctc atgctcgcct atatgaacca
     2881 ggaatccgtc cgaaagacga ttgaaacgaa agaaacttgg ttttacagcc gttccaggca
     2941 ggagctatgg aataaagtcg caacgtccgg caatcggcaa atcgtcaaac ggatatcctt
     3001 cgactgtgac caggatgcga tcctccttca agtgaaacct gtaggacccg cttgccatac
     3061 aggtgaaacg acttgcttct atacaagcga ttttgaagaa gggaattctg ttcgcggggt
     3121 cgtgcatgaa gtcgttgatg aaatcaagga acgccgtgca aatccggtag aaggttccta
     3181 tacatcctat ttgttccggg aaggcgtaga caaagtgctg aaaaagattg gggaagaatc
     3241 aacggaagtc gtcatcgggg caaaaaatcg ggataaggac gaattgacga atgaacttgc
     3301 cgacttgacg tatcatacac ttgtccttat ggaactgctt gacgtgtcgg tcgatgacgt
     3361 aaaggcgatc cttcgggata gacggccgca taaggaagtg gtgccgaatg aataaatact
     3421 ggagcgaaat cgtgaaaagg accgaaccgt atgtaccggg cgaacaattg aatgacccag
     3481 gcatcatcaa attgaataca aatgagaatc catatcctcc gagcccgctt gtaaaagaag
     3541 ctatattgga agagctggat ggtcagcttc gtttgtatcc atccccgaca gcagacggat
     3601 tgcgggagac aatcgccgag tcggttggtg tatcaaagga agaagttttt gtcggaaatg
     3661 gttctgatga agttctcgcc ttttccttca tggctttctt ccaaccaggc aaaccgatcc
     3721 gctttccgaa catcacgtat agcttctatc cggtttacgc aaaactgttt gacattccat
     3781 atgaactcgt ccgtctaaat gaagacttca gcttgccagt agaatctttc ttcaactcgg
     3841 aaggcggcgt catcctgccg aatccgaatg cgccgacgag cctttatatg gagctctcat
     3901 caattgaaga aattctaatc ggcaaccctg atacagtcgt catcatcgat gaggcatata
     3961 tcgattttgc gtccgagtcc gctgtggaat tggtgaagtc gtatggaaat ctccttgtcg
     4021 tccagacgac ctccaagtcc cgctcgcttg caggcatgcg cgtcggctat gcgatcggcc
     4081 atccggattt gatcgaggca gttgtgcgga tcaaggattc attcaattcc tatacaatcg
     4141 acaggcttgc aatcgccgga gcaacggcag cattcaaaga tggagcatat ttcgataaaa
     4201 cgataatgaa aatcaaagag acacggtctc gtttcataga agctatgaag ggaatgggat
     4261 ttcacgtatt accttcacaa gcgaacttcg tcttcgtttc gcattccgat atggcagcct
     4321 ccgacttgta taggcaatta aaagaagaag gtatcctcgt ccgtcatttc aatcaacc
//
LOCUS       DASULI010000440         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_558968, whole
            genome shotgun sequence.
ACCESSION   DASULI010000440 DASULI010000000
VERSION     DASULI010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558968"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFX34_09570"
     CDS             complement(<1..207)
                     /locus_tag="VFX34_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565200.1"
                     /translation="MSEEWFLTVITTRADFITAQSMLITTRMDFITTQSMFITTRPFL
                     TTLDGRFITNSWRVITDLDEFITDS"
     gene            complement(200..1279)
                     /locus_tag="VFX34_09575"
     CDS             complement(200..1279)
                     /locus_tag="VFX34_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HEX5565201.1"
                     /translation="MITKKLAEEIVEQTMVRLNRNLNVMDTDGMILASGEKERVDRIH
                     EGAAHVAKTRLPLWIDEQNTSDWHGAKPGVNMPIHYKDRLIGVIGITGNPEELKDIAT
                     LVQLTTEMMVHQALITSESEWKRKIKELVFEELTDGGALSPIVIDRLALLEFNMAGPY
                     LTLLVQTKNLPNSPQRLIETMEEQFEKNTALIGHSQLNELFIVLSEVNMSVLELKLLR
                     ILALFQKDSSVQIGVGLPVDKLEGIAHSYETAKYAIQYGQSNEQIHQYDDIELIALLK
                     SRQSPLKERFAARILDELNEQLIETLEAFFEHDQAIGETAASLAIHRHTLTYRLRKVK
                     ELTSLDPTQFRDAVLFHIAMLVRDE"
     gene            1418..>2044
                     /locus_tag="VFX34_09580"
     CDS             1418..>2044
                     /locus_tag="VFX34_09580"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX5565202.1"
                     /translation="MKVIVAPDSFKGSLTAIEAAEAMAAGICDVNPDIQTVLLPAADG
                     GEGTMRSLVDATKGEYVSVSVEDPLGRPIRATYGILGDGETCAIEIAEASGLMRLQEH
                     EKNPHIASSYGTGELIAHALDNGYRKFIIGLGGSATNDGGAGILQALGMRLLNREGNE
                     LPKGGGGLEHIYSIDSVKWDARIAESEFLIASDVKNPFVGSEGASAIFG"
BASE COUNT          536 a          463 c          513 g          532 t
ORIGIN      
        1 agagtcggtg ataaactcgt ccaagtcggt gataactcgc caagagttgg tgataaaacg
       61 gccatctaaa gtggtaagga atggtcgagt ggtgataaac atgctctgag tggtgataaa
      121 gtccatgcga gtggtgataa gcatgctctg agcggtgata aagtcggccc gagtggtgat
      181 aaccgtcaga aaccactctt cactcatccc tcacaagcat tgcaatgtgg aataaaaccg
      241 cgtcgcgaaa ttgggtcgga tccaatgatg tcagttcttt cactttacgc aatcgatacg
      301 tcaatgtgtg gcgatggatg gcaagggaag cagccgtttc accgatcgct tgatcatgtt
      361 cgaaaaaagc ttccaacgtt tcgatgagct gctcgtttaa ctcgtccaga atccgcgcgg
      421 caaagcgttc ctttaacggg gattgacggc ttttcaagag agcaatcagt tcgatgtcat
      481 catattgatg gatctgttcg tttgattgcc catattgaat tgcatatttc gccgtttcat
      541 acgaatgggc gatgccttcc aatttgtcga caggcagacc gacgccgatc tgaacagaag
      601 aatccttttg aaataaagca aggattctca gcaacttcaa ctcaagcaca gacatgttca
      661 cttcagataa tacgatgaac agctcgttca attgcgagtg gccgatcagc gcggtattct
      721 tttcgaactg ctcctccatc gtttcaatca accgttgagg ggaattgggc agatttttcg
      781 tttggactaa taacgtcaaa taaggtcctg ccatgttgaa ttccaataag gcgagccggt
      841 cgatgacaat cggtgaaagt gcgccaccat cggtcagctc ctcaaaaacg agctccttga
      901 ttttccgttt ccattcagac tccgatgtaa tgagtgcctg gtggaccatc atctcggtcg
      961 tcaattggac gagcgtcgcg atgtctttca attcttccgg attgcctgta atcccgatga
     1021 cgccgatcaa ccggtcctta taatggatgg gcatgttcac gccgggcttt gcgccatgcc
     1081 agtccgaagt gttctgttca tcaatccaaa gcggcagcct cgttttggca acatgcgcag
     1141 ctccttcatg gattcggtcg acacgctctt tttccccgga agccaaaatc attccatctg
     1201 tgtccatcac attcaagttc ctattcaacc ggaccatcgt ctgttccacg atttcctccg
     1261 caagcttttt tgtaatcaag aaatcaaccc cttgtataaa aaacgtcaat aattccttat
     1321 gaaacttata catcaagtat aatgtatttt cattttcaaa acatataatt aaatgaaacc
     1381 gctttatttt ttaattttta ctttggaggt gcttcccatg aaggtgatag tggcacctga
     1441 ttcgtttaaa ggaagtttga cagctatcga agccgcagag gcgatggcag caggtatatg
     1501 tgacgttaat ccggacatcc agaccgttct gctgccggca gcggacggcg gtgaaggaac
     1561 gatgaggagt ttggtcgacg ctacaaaagg agaatacgtg tctgtaagcg tggaagaccc
     1621 gctcggacga ccgattcgcg caacatacgg cattctcggg gacggcgaga cgtgcgcaat
     1681 cgaaattgct gaagcatcgg gacttatgag gctgcaggaa catgaaaaaa atccacacat
     1741 cgcatcttcc tacggaaccg gtgaattgat tgcccatgca ttggacaacg gttatcggaa
     1801 attcatcatc ggcctcggag gaagcgcaac gaatgacggg ggtgcgggaa tcctccaagc
     1861 gctcggcatg cgtttgttga atagagaagg gaatgaactg ccgaaagggg gaggcggact
     1921 cgagcatatt tactcgatag atagtgtgaa gtgggatgcc cggattgcgg aaagtgagtt
     1981 tttaattgcg agtgatgtga aaaacccatt tgtcgggagt gaaggtgcct cggctatttt
     2041 cggt
//
LOCUS       DASULI010000441         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_139131, whole
            genome shotgun sequence.
ACCESSION   DASULI010000441 DASULI010000000
VERSION     DASULI010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139131"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFX34_09585"
     CDS             complement(<1..701)
                     /locus_tag="VFX34_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEX5565203.1"
                     /translation="MQTLRNGLIAGLKTTWTLGKIIFPITVLVVILQHTPGLPWIVKL
                     ISPFMGLFGLSGEAAIPLVLGNALNLYAGIAGILSLELTVKEVFILAVMLSFAHNMFI
                     ETGVALKVGVKLWIVLVVRFGLAAFSGIIINLFWKGGSEIAKYGIAPEAATVPDGWLE
                     IGLLGLQKASFGVLQLALIVIPLMIIVQFLKDRNYLQKFSEKLAPFTKVIGVQPNASM
                     TLVAGLVIGLAYGAGV"
     gene            complement(717..1523)
                     /gene="lgt"
                     /locus_tag="VFX34_09590"
     CDS             complement(717..1523)
                     /gene="lgt"
                     /locus_tag="VFX34_09590"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5565204.1"
                     /translation="MFDLLTIDPTAFSLGPIDIRWYGILITTGIILAFIVVQKEMVKR
                     GMHPDFLTDLLIWAVPISIVSARIYYVIFSWDSYKDNPIDVIKIWEGGIAIHGALIGA
                     FLTTYFYTKRRGISFWKVVDIAVPGILIGQIIGRWGNFVNQEAHGGPVSQKFLENTWI
                     PDWIMNQMTIDGVTYHPTFLYESLWNVVGLIIILLLRKVPLKRGEMFFFYLTWYSIGR
                     FFIEGMRTDSLYGGDLRAAQIVSIIAILFSVGMFIWRRFIQKVNVKYQDK"
     gene            complement(1528..>2211)
                     /gene="hprK"
                     /locus_tag="VFX34_09595"
     CDS             complement(1528..>2211)
                     /gene="hprK"
                     /locus_tag="VFX34_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX5565205.1"
                     /translation="HNIECPIELRELAEERGIPVFQTEHPTTRFSGMLTNFLEGQLAP
                     MTTVHGVLVDVYGIGVLITGKSGVGKSETALELVKRGHRLVADDAVEIRQVAKNVLIG
                     NAPKLLTHMLEIRGVGIIDMMTLFGASAVKDDKRILMVIDLEVWDPNKVYDRLGIDEE
                     KMKIMDSDLTKLTVPVRPGRNLSVIIEVAAMDYRMKRLGINAAETFSKKLDLAISQEE
                     GNSDKEGIE"
BASE COUNT          590 a          604 c          457 g          560 t
ORIGIN      
        1 acgccggctc cgtaggcaag accgatgact aggccagcaa ccaatgtcat ggacgcattc
       61 ggctgtacgc cgataacctt tgtgaacggt gcaagtttct ctgagaactt ctgcaagtaa
      121 tttcggtctt tcaaaaactg gacgatgatc ataagcggaa tgacgataag cgcgagttgc
      181 aagacgccaa acgacgcttt ctgcaaaccg agcaatccga tttccagcca tccgtccgga
      241 acagttgccg cttcaggcgc tattccgtac tttgcaattt cgctgccgcc cttccagaac
      301 aaattgatga tgatgccgga gaacgccgcc aaaccgaagc gcacaacaag cacaatccat
      361 agttttacgc cgacctttaa agcgactccc gtctcgatga acatattatg tgcaaaactg
      421 agcataacag ccaaaatgaa aacttccttc accgtgagct cgagtgaaag gattccggcg
      481 atgcctgcgt ataaattcaa tgcattgcca agcactaatg gaatggcggc ttccccgctc
      541 aaaccgaata gtcccatgaa cggcgatatc aacttcacta tccaaggcaa tccaggtgtg
      601 tgttgcaaaa tgacaacgag cacagtgatc gggaatatga ttttccctaa cgtccacgtc
      661 gtctttaatc cagctatcaa cccatttcga agcgtctgca tttcccaatc cctcttttat
      721 ttatcttgat atttcacatt tactttctga atgaaccttc tccaaataaa cattcccact
      781 gaaaatagga tcgcgatgat ggatacgatt tgcgcggccc tgagatcgcc gccgtacaaa
      841 ctgtccgtcc tcatcccttc gataaagaag cgtcctattg aataccaggt caaatagaag
      901 aagaacatct ctccccgctt caatggaact ttccgtaata acagaatgat gatcagtccg
      961 acgacgttcc ataatgattc atagaggaac gtcggatgat aggttacgcc gtcgatcgtc
     1021 atttggttca tgatccagtc gggaatccac gtgttttcga gaaacttttg cgaaacagga
     1081 ccgccatgcg cttcctgatt gacgaagttc ccccatcggc cgatgatttg accgatcaaa
     1141 atgcccggga cagcaatgtc caccactttc cagaaggata tcccgcggcg tttcgtataa
     1201 aagtatgtcg ttaggaaggc gccgatcaaa gcgccatgaa tggcaatgcc gccttcccat
     1261 attttgatga cgtcgatcgg gttatcctta taggaatccc aggaaaatat gacatagtaa
     1321 atccttgcgg atacaatcga aatgggcacc gcccaaatga gcagatccgt taagaagtcc
     1381 gggtgcatcc cccgcttcac catctctttt tgaacgacaa tgaacgcaag gatgatgcct
     1441 gttgtgatta aaataccata ccaccggata tctattggcc ccaagctgaa ggccgtcggg
     1501 tcaatcgtca gtaaatcgaa catgagtcta ttctatcccc tctttatcag aattcccttc
     1561 ttcttggcta attgccaaat caagcttttt cgaaaatgtt tccgctgcat tgatgcctaa
     1621 tcgtttcatc cgataatcca ttgcggccac ttcgatgatg accgatagat tccgtccagg
     1681 tcttacaggg acggtgagct tcgtcaaatc cgaatccatg attttcatct tctcttcgtc
     1741 gatgcctaaa cggtcataga ctttattcgg atcccacact tccaaatcga tgaccatcag
     1801 aatccgcttg tcatccttga cggcactcgc gccgaacaat gtcatcatat cgataatgcc
     1861 tacgccccgt atttcaagca tatgggtcaa aagcttcggt gcatttccaa ttaagacgtt
     1921 ctttgcgact tgccgtattt caaccgcatc gtccgcgacg agacgatggc cgcgtttcac
     1981 gagctccaaa gcggtttcac ttttccctac accgctcttc cccgtaatca atacaccgat
     2041 cccatacaca tcgacaagca ctccgtgaac agtcgtcatc ggcgccaatt gaccttccag
     2101 gaaatttgtc agcatacccg aaaaccgcgt cgtcggatgc tcggtctgaa ataccggaat
     2161 gccccgctct tccgcgagct ccctaagttc aatcggacat tcgatgttat g
//
LOCUS       DASULI010000442         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1726588,
            whole genome shotgun sequence.
ACCESSION   DASULI010000442 DASULI010000000
VERSION     DASULI010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726588"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2144)
                     /gene="secA"
                     /locus_tag="VFX34_09600"
     CDS             complement(<1..2144)
                     /gene="secA"
                     /locus_tag="VFX34_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008407320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX5565206.1"
                     /translation="MLGVLNKMFDMNKRDVKRLEKIADQVEAYASEMERLSDDELKAK
                     TDEFKERLEKGETLEDLQVEAFAVVREASRRVLGMYPFRVQIMGAAALHEGNIAEMKT
                     GEGKTLTSTLAVYLNALAGKGVHVVTVNEYLASRDATEMGQLYEFLGLSVGLNLNSLS
                     KEEKREAYHADITYSTNNELGFDYLRDNMVLYTEHKVQRPLFYAVIDEVDSILIDEAR
                     TPLIISGQAEKSAELYKLANQFVRTLKKEEDYSYDETTKGVVLTEKGIERAEKAFSIE
                     NLFDLTHVRLNHAINQSLKAHASMHIDVDYVVQEGEVIIVDSFTGRLMKGRRYSDGLH
                     QAIEAKEGLEIQNESMTLATITFQNYFRMYEKLAGMTGTAKTEEEEFRNIYNMNVIAI
                     PTNRPIARIDNPDLIYASMDGKFRAVAEDIKNRHQKGQPVLVGTVAIETSEIISKLLT
                     KYGIKHEVLNAKNHGREADIISSAGEKGSVTIATNMAGRGTDIKLGEGVTELGGLAVI
                     GTERHESRRIDNQLRGRSGRQGDPGETQFYLSLEDELMRRFGSEQMKSMMTRLGMDDS
                     TPIQSKMVSRSVESAQKRVEGNNFDARKRLLQYDDVLRQQREIIYKERNQVLEAENMR
                     ELVENMIESVIDRTVSAYTADENPEQWNLRGLEDFISGNLLPEGDMTVADMEGKSADD
                     INALIKEKVKQRYDEKEEAMTSERMREFEKVVL"
     gene            complement(2327..2893)
                     /gene="raiA"
                     /locus_tag="VFX34_09605"
     CDS             complement(2327..2893)
                     /gene="raiA"
                     /locus_tag="VFX34_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5565207.1"
                     /translation="MLDFNIRGENIEVTPAIRDYVMKKIDKLDRYFTEGANATAHVNM
                     KVYNDKQTKVEVTIPMKNLKLRAEERHNDLYAAIDLITDKLERQIRKYKTKVNRKFRE
                     REGVAAFFASVNNDSNQSSVATEESEGEFDIVRTKQFDLKPMDQEEAILQMNMLGHNF
                     FIFTDAESNGTNIVYKRKDGKYGLIETQ"
     gene            complement(3027..3407)
                     /locus_tag="VFX34_09610"
     CDS             complement(3027..3407)
                     /locus_tag="VFX34_09610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5565208.1"
                     /translation="MFYKRSEYFRYTFGEPLQGEFRLLLSNGEERKSKPGECLLIDIS
                     PGGSKIYCEFDIPMDQKGVRMSLTFTLHEDPIETNGQIVWKKPSKSAYLYGVDFDPND
                     ETEELIVNELKLMRRSDATSTKKG"
     gene            complement(3476..>3871)
                     /locus_tag="VFX34_09615"
     CDS             complement(3476..>3871)
                     /locus_tag="VFX34_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565209.1"
                     /translation="LTAKTLTEIHANLADVNEMNEQTQVHLSNSTEKLSTQAETTITA
                     EKTIASMHGTFGELLDNFKMFQSKMESITRDVGDIGEMTMTFADLIADSSASLEEVNA
                     SIHASVADNERIVGTLDGTMHETRELLSN"
BASE COUNT          948 a          998 c          731 g         1194 t
ORIGIN      
        1 agaacgacct tctcaaattc gcgcatccgt tcggacgtca tcgcctcttc cttctcatca
       61 tagcgttgct tcactttttc cttaatcaat gcattgatgt catcagccga tttcccttcc
      121 atatcagcaa ccgtcatgtc accttccgga agcaagtttc cgctaatgaa atcctcaaga
      181 cctcttaggt tccattgttc aggattttca tccgctgtat acgctgaaac tgttcggtca
      241 atgacagatt caatcatatt ctcaactagc tcacgcatgt tttcagcttc aagcacttgg
      301 ttccgttctt tatagatgat ttcacgttgc tgacgcaaca catcatcgta ttggagaaga
      361 cgtttccgcg cgtcaaagtt atttccttct acgcgtttct gcgcagactc aacagagcga
      421 gagaccattt tagattggat cggcgtagaa tcatccatgc caagtcgtgt catcatcgat
      481 ttcatttgtt ccgaaccgaa acggcgcatc aattcatcct caagggataa gtagaattgc
      541 gtttcaccag ggtctccctg acgaccggaa cgtccacgga gctggttatc aatccgacgg
      601 gattcatggc gctctgtccc gataaccgct aaaccaccga gttcagtaac accttcgcca
      661 agcttgatgt ccgtaccacg acctgccatg ttcgtcgcaa tcgtgacaga tcctttttcc
      721 cctgcggagg agataatatc cgcctcacgg ccatggttct tcgcgttcaa aacttcatgc
      781 ttaatgccat atttcgttaa tagcttcgaa ataatttcag atgtctcaat cgcaacagta
      841 ccgacaagaa ccggctgtcc tttttggtga cgattcttaa tatcttccgc aactgcgcgg
      901 aatttgccat ccatggatgc ataaatcaaa tccggattat caatccgggc aatcggacgg
      961 ttcgtcggaa tcgcaatgac gttcatattg taaatgttac ggaattcctc ttcctcagtt
     1021 ttcgcagtac ctgtcatacc cgcgagcttt tcatacatac ggaagtagtt ctggaacgtg
     1081 attgttgcaa gcgtcatcga ttcattctgg atttccaagc cttcttttgc ttcgatcgcc
     1141 tggtgaagac cgtcgctata gcggcgccct ttcatcaaac gtccagtaaa ggagtcaacg
     1201 atgattactt cgccttcctg cacaacataa tccacatcaa tatgcataga agcatgtgcc
     1261 ttcaacgatt gattgatagc atgattcaga cgcacgtgag tcaggtcaaa caggttttca
     1321 atagagaacg ccttttccgc tctctcgatt cctttttccg taagaacaac acctttagtt
     1381 gtttcatcgt aggaatagtc ctcttccttc ttcaacgtac gaacaaattg attcgcaagt
     1441 ttatagagtt cagcggactt ttcggcctgc cccgaaatga tgaggggagt acgtgcctca
     1501 tcaattaaaa tcgagtcaac ctcgtcaata accgcataga aaagaggtcg ctgtacctta
     1561 tgttcggtat agagcaccat attatcacga aggtaatcaa acccaagctc gttgtttgtg
     1621 ctgtacgtaa tatcagcatg atacgcttcc cgtttttcct ctttcgacag actatttaaa
     1681 ttgagaccta cagataaacc tagaaattca tacagttgtc ccatttctgt tgcatcacgg
     1741 ctcgccaagt attcgttaac cgttacaaca tgaacacctt ttccagcaag cgcatttaag
     1801 taaaccgcta gcgtcgacgt taacgtcttc ccttcaccgg ttttcatctc tgcaatattt
     1861 ccttcatgaa gggcagcagc gcccataatt tgcacacgga atggatacat gccgagcaca
     1921 cggcgcgatg cctcacgaac gaccgcaaac gcttccactt gaagatcctc aagtgtctcg
     1981 cccttttcca atcgttcttt aaattcatca gtttttgcct tcagctcgtc atctgaaaga
     2041 cgttccattt cagatgcgta cgcttccact tgatctgcaa ttttctcaag acgtttcacg
     2101 tctcgtttat tcatatcaaa cattttattc aatacgccaa gcatattagt cacatcctca
     2161 ttaggttcaa ccttcatttt accactgcaa aaagcgtggc gcaaatgaac ccgtctgccg
     2221 ttttgtcaca cttgtttcgc tcagatttcc ggaccttttc tgggttctaa atacaaagaa
     2281 gccccccaga aggcattcac cacctgggga gcttcctacc atctatttat tgagtttcaa
     2341 tcaacccgta ttttccatct tttcgtttgt atacaatatt cgtgccgttc gactctgcat
     2401 ctgtaaagat gaagaaatta tggccgagca tattcatttg gaggatcgcc tcttcctgat
     2461 ccattggctt taggtcgaat tgttttgtcc gtacgatatc gaattcgccc tcagactctt
     2521 ctgtcgccac acttgattga ttcgaatcat tattgacaga agcgaaaaat gcggcaacgc
     2581 cttcccgctc acggaatttg cggttcactt ttgttttgta cttccggatt tgtcgttcca
     2641 gtttatctgt aatcaaatca attgctgcat aaaggtcgtt gtgccgttct tctgcacgga
     2701 gtttcaggtt tttcattgga attgttactt cgacttttgt ctgtttgtca ttgtagactt
     2761 tcatgttgac atgagctgtc gcattcgcac cctctgtgaa atatcgatcg agtttatcga
     2821 tctttttcat aacgtagtcg cgaatggctg gagttacctc aatattttcc ccacggatgt
     2881 tgaagtctag catacgaact cctcctttag ttagccttat ctactacttc tacatgaacc
     2941 agtccaaatc ctttaccatc ctgcaaatta tccaggaatt tgtcgaaact ggtcacactt
     3001 cagacaatga agttatccaa tcctccttac ccttttttcg tacttgtcgc atcggaccgt
     3061 cgcatcagct tcagttcatt cacgatcagt tcttctgttt catcgttcgg atcgaaatcg
     3121 acaccataaa gataagcgct cttcgacggt tttttccaga caatctgccc attggtttca
     3181 atcggatcct catggagtgt aaatgtgagg ctcatcctga cccccttttg atccataggg
     3241 atatcaaact cgcaatagat tttggagccc cccggactaa tgtcgatcaa caggcattcc
     3301 ccgggttttg atttccgttc ttccccgttg ctcaaaagga gcctaaactc cccttgcagt
     3361 ggttcaccga acgtatatct aaagtattcc gatcgtttgt agaacatgaa caaacactcc
     3421 gatccgatag tagtactact acccactttt attataacat aaaatttcta acttttcagt
     3481 tactaagcaa ttccctcgtc tcgtgcatcg ttccatcaag ggttccgacg attcgctcgt
     3541 tatccgccac ggaagcatgg atggatgcat tcacctcttc taaagaggca ctggaatcag
     3601 cgatgagatc cgcaaacgtc atcgtcattt ctccgatgtc cccgacatct cgcgtaatag
     3661 actccatttt tgattggaac atcttaaaat tgtccaacaa ctccccgaag gttccatgca
     3721 tggacgcaat agttttttct gctgtgatgg ttgtttccgc ctgcgtagaa agcttttcgg
     3781 tactgttcga gagatgaact tgtgtttgct cgttcatttc attgacatcc gctaggtttg
     3841 catggatttc agtcaacgtc ttcgctgtta a
//
LOCUS       DASULI010000443         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_831481, whole
            genome shotgun sequence.
ACCESSION   DASULI010000443 DASULI010000000
VERSION     DASULI010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831481"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..1308)
                     /locus_tag="VFX34_09620"
     CDS             complement(379..1308)
                     /locus_tag="VFX34_09620"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX5565210.1"
                     /translation="MTPKTFVGLYDTEPALLAAIEELDAKGIDPENMYVIARNEEDVD
                     MLRRRTSEDIQSAPSNWLDRFIGYISGENHVRSMLVEVGFDEADLRRYEAEIKQGKWL
                     LYVDGEPEKSAYEINEERQKAETNPFNHPVSEAERLNWQDDSPVGIGEDGLPKNDYRD
                     SILTDGYEGNRPVAREDLLFGRAGAEALIDRSEYEPDGGNPSPPSQIRGTPSKERAAR
                     RSREVNVADRGRHDVIPDPRNFMESQMDRQDAMSEAANFDESPGELDSIQGIYSEDVA
                     GPKKPIVIDLRNVGKAEEYSDTWLQPKNHDPDK"
     gene            1742..>3515
                     /locus_tag="VFX34_09625"
     CDS             1742..>3515
                     /locus_tag="VFX34_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX5565211.1"
                     /translation="MNDKEVMGCRLDMTQRARKEIGNWAIDENELFSMQAHFTVYIEK
                     LPAKQDNMAFSFYFDPEENPQLSNHLNERVAVMECVYKNEGFLATSFRVKGRKEPVRT
                     NRRIGVRLAFAVNRRTGSPMPIQLYTSLRELPIAQERSEYVEKRIKSWEGYLRIEEKN
                     ADVADVTAHFSQPVVNESFSQLTIICGGLEAKDWGAITGFSAKLKGSNNDLGTVVDVN
                     RGRRSVKIELNRKNQAIARRGSLLPNGAREIIFSNFAELSQIRRLRKGFKDLQDGFAA
                     NPNLEKVLFEERPVVQITNKRMELEFHHPLNEFQQEAVTGAMSAHDLYVIQGPPGTGK
                     TTVISELCHQLMKAGQRTLVASQSNLAVDNALGRLLADPGIRILRYGRTESIEEEGKR
                     FIEENVASYWRDETLTVVNEQREKRAEREREISEEIDRLQEKLQQLHAEREVMEKRIA
                     EKKAAETESNDLRLQQGSLNKLQAKLEEKLNSIAHQLEELNLTLANLTSEKNRLHEEL
                     QAASIGPELNEKMAAAELELSGNRKKLRYFQTVEAIGYAEMEIRRLTDEASSNRLKTG
                     PLPRFIEELSGVRKLQELEGLFTSH"
BASE COUNT          938 a          865 c          891 g          821 t
ORIGIN      
        1 cgattcaact ctttttgttt ctgtatttta gtcactcccc aaatcgcaat ggcaatgcca
       61 ataaagaaaa cagggaattt aattaacaat ccaatgaaca ccaatgcagc aataaccgtt
      121 aagaaacctt tcaaatctgc aatcctccac tactttaaaa tgcgatgttt ttaacatcat
      181 gcttcagcct agggaatccc ctttgcaaaa gcactctatt attatagcag gaaagtttgg
      241 aggacacgag agattgaata aaatggataa aaataaccgt tttgagcgtg tttcatcgga
      301 ttatgatcac ttaaacggat ttataagcgt agccacacaa aaagaccaca cacccgtaag
      361 tgcatggtcg atcagctttc atttatccgg gtcatggttc tttggctgaa gccatgtgtc
      421 gctgtactct tccgctttgc cgacattccg caagtcgatg acgatcggtt tcttcggccc
      481 ggcgacatcc tccgagtaga tgccttgaat cgaatcaagc tctcccggtg actcatcaaa
      541 attggccgcc tccgacatcg catcttggcg atccatctgg ctttccatga aattgcgcgg
      601 atcggggatg acgtcgtggc gcccccgatc ggcaacgttg acctctctgc tccgacgggc
      661 cgcacgctcc ttggatggtg tgccgcggat ttgcgaagga ggggaagggt tgcccccatc
      721 cggttcatat tcggatctgt cgattaaagc ctcagcccct gcccggccga acagaagatc
      781 ctcacgggcg actggcctgt tgccttcata gccatctgtc agtatgctat cccgataatc
      841 atttttcggc aagccatcct ccccgatgcc tacgggcgaa tcatcttgcc aattcaaccg
      901 ttcagcttcc gaaaccggat gattgaatgg gtttgtttcc gccttttgcc gttcctcgtt
      961 gatttcataa gcggatttct ccggttcgcc atcgacatac agcagccatt tgccttgttt
     1021 gatttccgct tcatatcgcc tcaaatccgc ctcgtcaaag ccgacttcga caagcatgct
     1081 gcgcacatgg ttctctccgg aaatataacc gatgaagcgg tccagccaat tcgacggcgc
     1141 cgactggata tcctcggatg tcctgcgccg cagcatgtcg acatcctcct cattccttgc
     1201 aatgacatac atattctccg gatcgatccc ttttgcatcc aactcctcaa tggccgcgag
     1261 aagcgccggt tctgtatcgt acaacccgac aaacgttttt ggggtcatat tcattcactc
     1321 cttctatctt ttgaaaaaca gtcgcaacct acctatgcaa tacccgaatc ttcaaaatgg
     1381 gaaacgatta taacaagttt ctatttccga tttctaatga atccgcaccc gattttgttg
     1441 tatgatagaa cgtatggttt tattttgatt catcaggatt atactcctgc tgaatcaaaa
     1501 taaagcctcc ggcggatgtc agggattttg tagggaataa ggaaggcaac ctaagttcgc
     1561 cgcgtcctgc aagtgcctta atttctgcaa agaacgcaga aatacggcaa atcgatccct
     1621 tcgctgttcg attggcaacg gttgcatgac caacatcctg ttggcctcaa gcgcaattca
     1681 aaatcccgac gcaattacgc cagggcgaaa ttgatttagg aacggaaagg aggatgtcat
     1741 gatgaacgat aaggaagtga tgggctgccg tcttgatatg acacagcgag cgcgcaagga
     1801 gatcgggaac tgggcgatcg acgaaaatga gctcttttcc atgcaggccc atttcaccgt
     1861 ctacatcgaa aagcttcccg caaaacagga caacatggca ttctccttct acttcgaccc
     1921 ggaggaaaat ccgcagctct ccaaccactt gaatgaacgg gtcgccgtca tggaatgcgt
     1981 ctacaaaaac gaaggcttcc tggcgacaag cttcagagtg aaagggcgta aagagccagt
     2041 tcggacgaat cgccgaattg gcgtccgcct tgcatttgca gtcaaccggc gtaccggaag
     2101 tccgatgccg atccagctat atacgagcct tcgggagctc ccgattgcgc aagagcgttc
     2161 ggaatatgtg gaaaaacgga tcaagagctg ggaaggctac ttacggatcg aggagaaaaa
     2221 tgcagacgtc gcggatgtga ccgcccattt ttcacagccg gtcgtcaatg aatcattcag
     2281 tcagctgaca atcatttgcg gtggattgga agcgaaagac tggggtgcaa tcaccggatt
     2341 cagcgccaaa ttgaaaggtt cgaacaatga tcttggaacg gtcgtcgatg tgaatcgggg
     2401 aagacggtcg gtgaaaatcg agctgaaccg aaaaaaccag gcgattgcaa ggcgcggctc
     2461 tttattgccg aatggcgcac gagaaatcat tttcagcaat tttgcggaat taagtcaaat
     2521 caggcggctg cgaaaaggct tcaaggatct gcaggacgga tttgcggcga atccgaattt
     2581 ggagaaggtg ctgttcgagg aacggcccgt cgtccaaatt acgaataaga ggatggagct
     2641 cgagttccat catccattga atgaattcca acaggaagcg gtcaccggcg cgatgtctgc
     2701 gcatgacttg tatgtcatcc agggtcctcc cgggacaggg aagacgacgg tcatttcgga
     2761 gctttgccat cagctcatga aggcggggca acggacgctt gtcgcctccc aatcgaattt
     2821 ggctgtggac aatgcgctcg gaaggctgct ggccgatccg ggcatccgga ttttacgcta
     2881 tgggcggacg gagagcattg aggaagaagg gaagcggttc atcgaggaga atgtcgcttc
     2941 ctactggcgg gacgaaacat tgactgtggt caatgaacag cgggagaagc gcgcggaacg
     3001 ggagcgggaa atctccgagg aaatcgatcg cctacaagaa aagctgcagc aacttcatgc
     3061 agaacgcgaa gtaatggaaa agcggatcgc cgagaaaaag gctgccgaga cggaaagcaa
     3121 tgacttgcgt ttgcagcagg gttccttgaa taaattgcag gcgaaattag aagagaaact
     3181 gaactcaatt gcacaccaac tggaagagtt gaacctgacg ttagcaaatt tgacttccga
     3241 aaagaacaga ttgcatgaag agcttcaagc cgcttcaatc ggtccggaat tgaatgaaaa
     3301 gatggctgcc gcagagctgg aattgtcggg gaaccgcaaa aaactccggt atttccaaac
     3361 agtcgaagcg atcggctacg cagaaatgga aattcgcaga ttgacggatg aggcgtcatc
     3421 gaaccggttg aagaccggac cgttgcctcg ttttatcgaa gaattatcgg gtgtacggaa
     3481 actgcaggag ctggaagggc tgttcacttc gcaca
//
LOCUS       DASULI010000444         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_476504, whole
            genome shotgun sequence.
ACCESSION   DASULI010000444 DASULI010000000
VERSION     DASULI010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476504"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFX34_09630"
     CDS             <1..501
                     /locus_tag="VFX34_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5565212.1"
                     /translation="VFASLLQYWVASQWHLLPVGLWNDGWKSSILPSIALAMGPLALS
                     ARFIRTEMIEVLSSDYITLAKAKGANGFEIAFKHAFRNALIPLVTVLGPVAAGIVTGS
                     LVVEQNFAIPGIGEQFVSSIMTNDFATIMGTTLFFSALLIFIIFIVDLLYGIIDPRIR
                     LSGGKS"
     gene            501..1523
                     /gene="opp3C"
                     /locus_tag="VFX34_09635"
     CDS             501..1523
                     /gene="opp3C"
                     /locus_tag="VFX34_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HEX5565213.1"
                     /translation="MRKEELQQLPPEKFERITIDSSSAERITKPSMSFWQDAWLRLRK
                     NKAAIVSMVILVLLIIMALIGPHLNGHDGDEQILRHANLPPKIPGIEKLGIFDGMGKL
                     GGETVNLYEIKKVDEYYWFGTDGLGRDLFTRVWEGTQISLLIAFVAAAIDVVIGVLYG
                     GISGYFGGRVDDIMQRIVEILIGIPTLIIVILMLLIMEPGLTAIIIAISLTGWIGMSR
                     IVRAQILKYKGQEFVLASRTLGASDTRIIGKHLLPNIMGIIIINTMFTIPTAIFFEAF
                     LSFIGLGLQPPAASLGTLINDGYKVMEHQPYFLMFPAVIISVLMIAFNLIGDGLRDAL
                     DPKMKD"
     gene            1540..2604
                     /locus_tag="VFX34_09640"
     CDS             1540..2604
                     /locus_tag="VFX34_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5565214.1"
                     /translation="MEKILQVKDLELSFHTFAGEVKAIRGVSFDLNKGETLAIVGESG
                     SGKSVTTKSIMRLLPESSSEFKNGEILFNGNDLTKLSDKQMQKIRGKEISMIFQDPMT
                     SLNPTMTIGKQVMEPILKHQKVSKAKAREVGIELLNMVGIPNAENRFKMYPHQFSGGQ
                     RQRIVIAIALACNPKILIADEPTTALDVTIQAQILELMKDIQKKVDTSIIFITHDLGV
                     VANVADRVAVMYGGRIVEVGTVDEIFYNPQHPYTWGLLSSMPSLDLTEEKLYAIPGTP
                     PDLLNPPVGDAFALRSEYALKIDMEQPPPFFKVSDTHYAATWLLHPNAPQVDPPKTII
                     DRMKTFRGSRYYDQSNGGNA"
     gene            2604..>3125
                     /locus_tag="VFX34_09645"
     CDS             2604..>3125
                     /locus_tag="VFX34_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5565215.1"
                     /translation="MAEKLLEVKNLKQYFNAGKPSEVRAIDNISFDIYRGETFGLVGE
                     SGCGKSTTGRTIIRLYDATDGEVLYDGVSVHANKSKSEVKALNQKMQMIFQDPYASLN
                     PRMKVLDIIAEGLDVHGLAKNPKERKQRVVELLETVGLNKEHADRYPHEFSGGQRQRI
                     GIARALAVNPEFII"
BASE COUNT          890 a          626 c          763 g          846 t
ORIGIN      
        1 gtattcgctt ccttattgca atattgggtc gcttcccaat ggcacttatt gccggtcgga
       61 ctttggaatg acgggtggaa atcaagcatt ctgccttcca tcgcattggc gatgggacca
      121 cttgcccttt cggcacgttt catccgtacc gaaatgatcg aagtattgag ttccgactat
      181 ataacattag caaaagcgaa aggtgccaac ggttttgaaa tcgcattcaa acatgcattc
      241 cgaaatgcgc taattcctct cgttacagta ttaggaccgg tagcagccgg tattgtcacc
      301 ggttccctag tagtagaaca gaactttgca attccaggaa tcggggagca gttcgtatcc
      361 tccatcatga caaatgactt tgcaacaatc atgggtacga cactattctt ctctgcattg
      421 ttgattttca ttatcttcat tgtagacctt ctttacggaa tcattgatcc gcgaatccgt
      481 ttgtctggag gtaaaagtta atgcgaaaag aagaacttca acaattgccg ccggaaaaat
      541 tcgagcgtat tacgattgat agcagtagtg ctgaacgtat tactaaacct agtatgagtt
      601 tttggcaaga tgcatggcta agattgcgca aaaacaaagc ggcgatcgtc agcatggtga
      661 ttctagtttt gctgatcatt atggctttaa ttggaccgca tctaaatggc cacgatggtg
      721 atgagcaaat tttacgtcac gctaacttgc cgccaaaaat tcctggaatt gaaaaattag
      781 ggatatttga cggaatgggt aaattaggcg gggaaacggt aaacctatac gagattaaaa
      841 aagtggatga gtattactgg tttggaacgg atggacttgg ccgtgacttg tttacccgtg
      901 tttgggaagg gacccagatt tctttattga tcgcatttgt tgctgcggcg attgatgtgg
      961 tcatcggtgt cctgtacgga ggtatatcgg ggtatttcgg cggtcgtgta gatgatatca
     1021 tgcaacggat agtcgaaata ctgatcggga tcccgacact tattatcgtc attttgatgc
     1081 tattaattat ggagcctggc ttaaccgcca tcatcattgc gatatccctc acaggctgga
     1141 ttggaatgtc ccgtattgta cgtgcccaaa ttttgaaata taaagggcaa gagttcgtac
     1201 tggcttcgcg gacattggga gcgagtgata cgcggatcat cggcaaacat ctattgccga
     1261 atatcatggg aatcattatc atcaatacaa tgtttacgat tcctaccgcc attttctttg
     1321 aggcgttctt aagctttatc gggcttgggc tgcaaccacc cgcagcttca ttaggtacat
     1381 tgatcaatga tggctacaaa gtgatggaac accagccgta tttcctaatg ttcccggctg
     1441 tgatcatcag tgtcttgatg attgcattta acttgatcgg tgatggattg cgcgatgcgc
     1501 tcgatccgaa aatgaaagat tgattggagg aaagccaaga tggaaaaaat attgcaagta
     1561 aaagacttgg agctttcctt ccacacgttt gccggtgaag tgaaagcaat ccgaggtgtc
     1621 agctttgatt tgaataaagg tgagacgctg gcgattgtcg gcgagtcggg ttccggaaaa
     1681 tcggttacga caaaatcgat tatgcgttta ttgcccgagt ccagctctga atttaaaaat
     1741 ggtgaaattc tatttaacgg aaatgatctt acgaaacttt ccgataagca gatgcaaaaa
     1801 atcagaggga aagaaatctc aatgattttc caggatccaa tgacatcatt gaatccgaca
     1861 atgacaatcg gcaagcaagt gatggagccg attttgaaac accaaaaagt aagcaaggca
     1921 aaagcgagag aagtcggcat tgaattatta aatatggttg gaattccgaa tgcggaaaac
     1981 cgttttaaaa tgtatcctca ccaattttcc gggggacaaa gacagcgtat cgtcattgcg
     2041 atagcgcttg catgtaatcc gaaaatcttg attgccgatg aacctacaac tgcacttgac
     2101 gttacaatcc aagcgcaaat ccttgaactg atgaaggata tccagaaaaa agtcgatacg
     2161 tcgattattt tcatcacaca tgatctcggt gttgttgcga acgttgcaga tcgggttgca
     2221 gttatgtatg gcggtcggat tgtggaagtg ggaacggttg atgaaatctt ctataacccg
     2281 cagcatccat atacatgggg ccttctaagt tcaatgccgt cattggattt gactgaggaa
     2341 aagctatatg cgattcctgg cacaccgcct gatttgttga atccgcctgt tggagacgcg
     2401 tttgcactac gtagtgaata tgctttgaag attgatatgg aacaaccgcc tccgttcttc
     2461 aaagtgagcg atacacatta tgccgcaact tggctgctgc atccaaacgc accgcaagtt
     2521 gatcctccga agacgatcat cgaccggatg aagacattcc gtggaagcag atattatgat
     2581 caatcgaatg gaggtaatgc atgatggcag agaaattgct tgaagtgaaa aacttgaaac
     2641 aatattttaa tgccgggaag ccttccgaag ttcgtgcaat cgataatatc agcttcgata
     2701 tttatcgcgg ggagacattc gggcttgtcg gtgaatcagg ctgtggaaag tcaacgacgg
     2761 gcaggacaat cattcgactt tatgacgcga ctgacgggga agttctttat gacggagtca
     2821 gtgtgcatgc caataaatct aaatctgaag tgaaagcttt gaaccaaaag atgcaaatga
     2881 ttttccaaga tccatacgca tcactgaatc cgcggatgaa ggtcctggat atcattgcag
     2941 aaggtctgga cgttcacggc ctggcgaaaa atccgaaaga acggaaacag agagtagtcg
     3001 aattgcttga aacagtcgga ttgaataagg aacatgccga ccgttatccg catgagtttt
     3061 caggtgggca gcggcaaagg atcggaattg cccgtgcgct tgccgtcaat ccggagttca
     3121 tcatc
//
LOCUS       DASULI010000445         3148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_461962, whole
            genome shotgun sequence.
ACCESSION   DASULI010000445 DASULI010000000
VERSION     DASULI010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461962"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..393
                     /locus_tag="VFX34_09650"
     CDS             124..393
                     /locus_tag="VFX34_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565216.1"
                     /translation="MYPVNQMPQNTLQPIVCPTQCRYHDQFFPVEQPYIHPIVNVNRT
                     HPVITPRHYYTETTENVMGAPVYPGQGPGFGRRGRGFFGFGRRPF"
     gene            complement(495..>3148)
                     /locus_tag="VFX34_09655"
     CDS             complement(495..>3148)
                     /locus_tag="VFX34_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX5565217.1"
                     /translation="HYHESPESLSQKLKGFAEKGWLNMVGGCCGTTPDHIRAIREAVD
                     SLSPRQKPEDGHEHAVSGIEPLLYEPSMRPLLIGERTNVIGSRKFKRLIVEEKFEEAS
                     EIARAQVRGGAHVIDICLANPDRDELEDMKNFMQEVVKKVKVPLVIDSTDEKVIEEAL
                     KFSQGKAIINSINLEDGEERFDAVLPLVKKFGAAVVVGTIDEVGMAVTRERKLEIAER
                     SYDLLVNKWGIAPEDIIFDPLVFPVGTGDAQYIGSAVETIEGIRLIKEKMPRGLTTLG
                     VSNVSFGLPPVGREVLNAVYLYHCTQAGLDYAIVNTEKLERYASIPDHEINLANDLLF
                     HTTDQTLADFTDFYRDKKKEKTEDDIPKTVPERLAYYVVEGTKEGLIPDLEAALKMYD
                     SPLDVINGPLMEGMSEVGRLFNDNQLIVAEVLQSAEVMKASVSFLEQFMEKKEDDSGK
                     GKIVLATVKGDVHDIGKNLVDIILSNNGYRVIDIGIKVTPATLIDVIRKEKPDIIGLS
                     GLLVKSAQQMVITAQDFKAAGIDTPVMVGGAALTRRFTETKIAAEYDGPVIYAKDAMQ
                     GLELANRLQGGDREALLNELVEKQEKRLETEAIRASRDTAVAVLPRPVKTVREDAPVF
                     IPSDLRRRVLRDYSVSHLYPYVNMRTLIGHHLGLRGNVDKMLANGEERAVQLHEMVTG
                     FLESGNLSASGMYQFFPAQADGDDVIIYDPEDAKTEIERFTFPRQSKEPFLCLADYLK
                     TIDSGEMDYVALMQVTAGHGVRDFANQLKAEGKFLESHAFQATALELAEGFAERVHQE
                     IRDQWGFPDATDFTMRDRFAAKYQGQRFSFGYPACPNLEDQAKLFGLLKPEDIGVHLT
                     EEFMMEPEASVSAIVFAHPDARYFNVD"
BASE COUNT          702 a          916 c          761 g          769 t
ORIGIN      
        1 gtcgctcgcc cgtatgcccc gtccattttc acatgtaata gatagagggg gtgagtgaag
       61 atgatgatgc accatggaca ctgtggaggc cgttgcggag ggcgctgcgg aggagcttgc
      121 ggaatgtatc ctgttaatca aatgccgcaa aacacactcc aaccgatcgt ttgtccgaca
      181 caatgtcgtt accatgacca gttcttccca gtcgagcaac cgtacattca tccgattgtc
      241 aacgtcaata ggacgcaccc ggttatcaca ccgcggcact attacacgga gacaacggaa
      301 aacgtaatgg gcgcgccggt ataccctgga caagggcctg gtttcggcag aaggggcaga
      361 ggcttcttcg gcttcgggag aagaccattc taatagcata aatgctgtcc tagcgtcgag
      421 cacttcgaca ttgggacagc ttttttattt tagagcaaaa aaagccgccc ccaagtagga
      481 ggcgactcaa ctagtcaatc tacattaaaa taccgtgcat ccggatgcgc aaatacgatg
      541 gccgacaccg atgcttccgg ctccatcatg aattcctcgg tcaaatggac gccgatgtcc
      601 tccggtttca acaagccgaa tagcttcgcc tgatcctcaa ggttcggaca agccgggtag
      661 ccgaacgaga agcgctggcc ttggtatttg gctgcgaacc ggtcgcgcat cgtgaaatcg
      721 gtcgcgtccg ggaaccccca ctggtcgcgg atttcctgat gcacacgctc cgcgaatcct
      781 tccgccagct ctagcgcggt cgcttggaac gcgtgactct cgaggaattt tccttccgcc
      841 ttcaactgat tcgcaaaatc ccggacgccg tggccagccg tcacttgcat aagcgcgaca
      901 taatccattt cgccgctatc gatcgtcttc aaatagtcgg cgaggcagag gaatggctct
      961 ttagattgtc gagggaatgt aaagcgctcg atttccgtct tcgcgtcttc tggatcataa
     1021 atgatcacat catctccatc cgcctgtgcc gggaaaaatt gatacatgcc ggaagccgac
     1081 agattgcccg actccaaaaa gccggtcacc atctcatgga gctgtaccgc ccgctcctcg
     1141 ccattcgcaa gcatcttgtc gacattgccg cgcaagccta aatgatgccc gatcagcgta
     1201 cgcatattga cgtacggata caaatgggaa acggaataat cccttagcac gcgtctgcgt
     1261 aaatcggacg ggatgaacac cggcgcatct tcccggaccg tcttcactgg cctcggcaat
     1321 accgcaacgg cagtatcccg cgatgcacga atcgcctcgg tctcaaggcg cttctcctgc
     1381 ttctcaacaa gttcattcaa taaagcctct ctgtcgccgc cttgcaatcg attcgctagt
     1441 tcaagccctt gcatggcgtc cttcgcataa atgacggggc catcatattc cgctgcaatt
     1501 ttcgtttccg taaatctacg agtcagcgcg gcgccgccaa ccattacagg cgtatcaatg
     1561 cctgccgcct tgaagtcctg cgcggtaatc accatttgtt gcgccgactt cacgagcaag
     1621 ccggacaggc cgataatgtc cggcttttct ttccggatga catcgataag cgtcgcgggt
     1681 gtcactttaa tgccgatatc gatgacacgg tacccgttat tgctcaaaat gatatcaacg
     1741 aggttctttc cgatgtcatg gacgtcccct ttgaccgtcg ccagcacaat cttcccttta
     1801 ccggaatcat cctctttctt ctccatgaac tgttcgagga atgaaaccga agccttcatc
     1861 acttccgcac tttgcaaaac ttcagcgacg atcagctggt tatcattgaa caggcgtccg
     1921 acctcggaca tcccttccat caacggtccg ttgatgacgt caagcggcga gtcgtacatc
     1981 ttcagcgcgg cttccaggtc ggggatcaac ccttccttcg tcccctcgac gacgtaatac
     2041 gcgagtcgtt caggaaccgt cttcggaata tcgtcctcgg tcttctcctt cttcttatcc
     2101 cggtagaaat cagtgaaatc tgcaagcgtt tgatctgttg tatggaacag aaggtcattt
     2161 gcaagattta tttcatggtc cggaatcgaa gcgtaccgtt ccagcttctc tgtattgaca
     2221 atcgcgtaat cgagtcccgc ctgggtgcaa tgatacaagt acaccgcgtt cagtacttcg
     2281 cggccgacag ggggcagccc gaaggaaacg ttgctcacac cgagcgtcgt caagcctctc
     2341 ggcattttct ctttgatcag ccggatgcct tcaatcgttt caactgcaga tccgatgtac
     2401 tgcgcatcgc ctgttccgac agggaacacg agcggatcga aaatgatatc ttcaggggcg
     2461 attccccatt tattcacaag cagatcatag gaacgttctg cgatttcgag cttccgctca
     2521 cgtgtcactg ccatcccgac ttcatcgatc gtcccgacga cgaccgcagc accgaatttt
     2581 ttcacgagag gcagcaccgc atcgaaccgt tcctcgccgt cctccaagtt gatggagttg
     2641 atgatcgctt tcccttgcga gaatttcagt gcctcttcga tgactttttc atcggtcgaa
     2701 tcgatgacga gaggcacttt cacctttttg acgacttcct gcatgaagtt cttcatgtct
     2761 tcgagctcat cccggtccgg attcgctagg cagatgtcaa tgacatgggc gccgccgcga
     2821 acttgggcac gcgcaatttc ggacgcttcc tcgaatttct cctcgacgat cagccgcttg
     2881 aatttcctgg acccgatgac attcgtccgc tcgccgatca ataacggccg catcgatggt
     2941 tcgtacaata acggttcaat gccggaaacg gcgtgttcat gtccatcctc tggcttctgc
     3001 cgcggtgaga gactgtctac cgcttcgcgt attgcgcgga tatggtcggg cgtcgttccg
     3061 caacagccgc cgaccatgtt cagccatcct ttttccgcaa atcctttaag tttctgcgat
     3121 aacgattcag gcgattcatg gtaatggc
//
LOCUS       DASULI010000446         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_131086, whole
            genome shotgun sequence.
ACCESSION   DASULI010000446 DASULI010000000
VERSION     DASULI010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131086"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFX34_09660"
     CDS             <1..276
                     /locus_tag="VFX34_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HEX5565218.1"
                     /translation="RSLGTLMYARLLPSEEAARLLSDVRLGIDLGLIKDVDVSVLNEL
                     MIFMQPGFLQQFAGSELTAEERDICRAKMFRERLRIENDPTDTTGNE"
     gene            504..>2119
                     /locus_tag="VFX34_09665"
     CDS             504..>2119
                     /locus_tag="VFX34_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX5565219.1"
                     /translation="MMFNRFTQRAQKVLQLAQEEAIRMKHDSIGTEHILLGLIREGGG
                     IAAKALEAIDVSFETIEEGIEKLVGTGSKDVGPIVHYTPRAKKVIELSVDESRKLGHS
                     YIGTEHILLALIREGEGVAARVLNNAGVSLNKARQQVLQLLGSNDSSMGSHGASNSAA
                     TPTLDSLARDLTEVAREGTLDPVIGRSKEITRVIEVLSRRTKNNPVLIGEPGVGKTAI
                     AEGLAQQIINNEVPEILRDKRVMTLDMGTVVAGTKYRGEFEDRLKKVMDEIRQAGNII
                     LFIDELHTLIGAGGAEGAIDASNILKPSLARGELQCIGATTLDEYRKYIEKDAALERR
                     FQPIQVDEPTVDESIQIINGLRDRYEAHHRVKITDEAIEAAAKMSDRYISDRFLPDKA
                     IDLIDEAGSKVRLRSYTTPPNLKELEAKLEAIRSEKNAAVQSQEFEKAASFRDKEQKM
                     KEELESTKAAWKEKQGKTESKVTVEDIAEVVAMWTGIPVAKIAETESVKLLNMEETLH
                     ERVIGQKEAVDAISRAIRRARAGLKDPKRP"
BASE COUNT          624 a          439 c          555 g          501 t
ORIGIN      
        1 cggtcactcg gaactttgat gtacgctcgt ttattgccgt cggaagaagc ggcgcgtctt
       61 ttatcggatg tccgcttagg aattgatcta gggctgatta aagatgtcga tgtctctgtg
      121 ttaaatgaac tcatgatatt catgcagcct ggattcttac agcaatttgc cggctccgag
      181 ctaactgcgg aggagcggga catctgcaga gcgaagatgt tccgggagag attgcgcatc
      241 gaaaatgatc caacagacac gacgggaaac gagtaaaata ggtgagcgtt ctgtttgcat
      301 gagcgctttc cccttagtat aattaagtca aagatagtca aagtcagtct atcttcgaca
      361 ggattttcta tacgtatctt gaatacgtat tatagagaag ttgtcccgta tggtttatta
      421 gaaacaggat agggaacgat ttcgttaggg acaaaactaa gatattaccg ttgatattag
      481 atcttcaaag gagaggactg tatatgatgt ttaaccgatt tacgcaacga gcacaaaagg
      541 ttttacagct cgcgcaagaa gaagcgattc gtatgaagca tgattcaatc gggacagaac
      601 acatcctact cggtctaatt cgagagggtg gtggaatcgc cgcaaaagca ttggaagcaa
      661 ttgatgtcag ctttgaaacg attgaagaag gcatcgagaa gcttgtaggc accggttcca
      721 aagatgtagg tccgattgtt cactatacac cacgtgcaaa gaaagtaatt gagctatctg
      781 tcgatgaatc aagaaagttg ggtcactcct atattggtac agagcatatc cttctcgcac
      841 tgatccgtga aggtgaagga gtggcggcac gtgttctgaa taacgctggc gtcagcctaa
      901 ataaagcgcg tcaacaagta cttcagcttc tcggctccaa tgattcttcc atgggcagcc
      961 atggggcatc caattcggct gctaccccta cacttgacag ccttgctcgc gatttaacag
     1021 aagtggcgag agagggtact cttgacccgg taatcgggcg aagcaaagaa atcacacgcg
     1081 ttattgaagt tctttcccgc cgtacgaaga acaaccctgt tttaattgga gagccgggag
     1141 tcggaaagac ggcaattgcg gaaggactcg cacagcaaat tattaataat gaagtaccgg
     1201 aaatcctacg tgacaaacgt gttatgacgc ttgatatggg tacggttgtt gctggtacga
     1261 aataccgcgg tgagtttgag gatcgtttga agaaagtcat ggatgaaatc cgacaagcgg
     1321 gcaatatcat cctcttcatt gacgagcttc atacattgat cggtgccggc ggagcggaag
     1381 gcgcaattga tgcatccaac attttgaagc catccctcgc gcgtggcgaa ctacaatgca
     1441 tcggtgcaac aacattggat gaataccgta agtacattga gaaggatgct gcgcttgagc
     1501 gccgtttcca gccaatccaa gtggacgaac cgactgtgga tgagtccatt cagattatta
     1561 atgggctgcg tgaccgctat gaagcgcatc accgtgtaaa gattacagac gaagcaatcg
     1621 aagcagcagc aaagatgtca gaccggtata tttctgatcg tttcttgccg gataaagcga
     1681 ttgacttgat agatgaagca ggctcgaagg tacgtcttcg ttcgtatact acaccgccaa
     1741 acttgaagga gcttgaggcg aagcttgaag caattcgctc cgagaagaat gctgcggtgc
     1801 aaagccagga gtttgaaaaa gcggcttcct tccgcgacaa ggaacaaaag atgaaggaag
     1861 agcttgagag tacaaaagct gcttggaaag aaaagcaagg taaaacagaa tcaaaagtaa
     1921 cggttgagga cattgcggaa gttgtcgcta tgtggacagg aattcctgta gcgaagatcg
     1981 cagagacaga atccgtcaaa cttctaaaca tggaagagac acttcacgaa cgcgttatcg
     2041 gccaaaaaga agcggtcgat gctatttccc gggcaatacg ccgtgctcgt gcagggttga
     2101 aagatccgaa acgcccaat
//
LOCUS       DASULI010000447         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_111441, whole
            genome shotgun sequence.
ACCESSION   DASULI010000447 DASULI010000000
VERSION     DASULI010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111441"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..644
                     /locus_tag="VFX34_09670"
     CDS             21..644
                     /locus_tag="VFX34_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5565220.1"
                     /translation="MENKREILTSVKDETLIEKRRNQMIRGAVKLFKEKGFHRTTTRE
                     IAKEAGFSIGTLYEYIRTKEDVLYLVCDSIYEQVNLRLSELNGQDGTIEGLRSAIGHY
                     YKLIDDMNDEFVVMYQESKSLPKDALQYVLTKEMEMVSLFEDRIRKCVQSGEIHLNEQ
                     EIPLAAHHVVVQGQMWAFRRWAIHKSYTLDQFISIQTAFLLHGVLGE"
     gene            666..>3350
                     /gene="icmF"
                     /locus_tag="VFX34_09675"
     CDS             666..>3350
                     /gene="icmF"
                     /locus_tag="VFX34_09675"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEX5565221.1"
                     /translation="MATAEIYRPKHHIRFVTASSLFDGHDASINIMRRILQASGAEVI
                     HLGHNRSVEEVVNAAIQEDVQGIAISSYQGGHVEYFKYMYDLLQEKGAPHIRIYGGGG
                     GVIIPREIKELHDYGIAWIFSPEDGRKLGLQGMINRMLEECDFSTAKENAVELANALS
                     TDRPEVLANLITVAEEAHSSGVDSETLLTQVRANSKNTPVIGITGTGGAGKSSLTDEL
                     IRRFLQELPEKKVAILSIDPTKQKTGGALLGDRIRMNAIFNKRVFMRSLATRGSKSEL
                     SGAIKDVLDVVKAAGYDLIIVETSGIGQGDAEITGVSDVSMYVMTSEFGAPSQLEKID
                     MIDYADLIVINKFERKGSEDALRQVQKQYQRSHLLFDESLDKMPVYGTIASQFNDKGT
                     NSLFAALISKLNEKCGTDWATTYEQFVKTQKQDVIIPNDRRHYLREITDTVRGYHKKS
                     EQQVEFARRLFQLEGALEAVKETSPDDALIQSLQSLADGVRNELSAESKRILENWEKL
                     KEAYSGDEFVTKIRDKEIRTILRTESLSGLKIPKVILPKFKDYGEILRWVYKENVPGS
                     FPYTAGVFPFKRQGEDPKRQFAGEGTPERTNRRFHYLSKDDDAKRLSTAFDSVTLYGE
                     DPDYRPDIYGKVGESGVSICTLDDMKKLYDGFDLCAPSTSVSMTINGPAPIILAMFMN
                     TAIDQQVRIREEEVGRPLTLEEFTVVRDQTLQVVRGTVQADILKEDQGQNTCIFSTEF
                     ALRMMGDIQQYFIDHKVRNYYSVSISGYHIAEAGANPISQLAFTLSNGFTYVEYYLSR
                     GMNIDDFAPNLSFFFSNGLDPEYTVIGRVARRIWAVAMREKYGANERSQKLKYHVQTS
                     GRSLHAQEIDFNDIRTTLQALMALQDNCNSLHTNAYDEA"
BASE COUNT         1008 a          732 c          829 g          781 t
ORIGIN      
        1 acgaagtgac ggaacgaatt atggagaata aacgagaaat cctgacttcg gtcaaagacg
       61 agacactgat cgagaaacgc cgtaaccaga tgattcgggg agcggtgaaa ctctttaagg
      121 agaaagggtt ccaccgaacg acaacaaggg aaatcgccaa ggaagcggga ttcagcatcg
      181 ggacgttgta tgaatatatc cggacaaagg aagatgtcct ctatctggtt tgtgacagca
      241 tttacgagca agttaatctt cggttatccg aattgaatgg acaggatggg acgattgaag
      301 ggcttcgttc ggcaatcggc cattactaca aactcatcga tgatatgaat gatgaattcg
      361 tcgtgatgta tcaggaatct aagtcgcttc caaaggatgc acttcaatat gttttgacga
      421 aggaaatgga aatggtctcg cttttcgaag accggatccg aaaatgcgtc cagtcaggcg
      481 aaatccatct gaatgaacag gagatcccac ttgcagcgca ccatgtcgtc gtccaaggcc
      541 aaatgtgggc cttccgcaga tgggcaattc acaaatcata tacactcgat caatttatta
      601 gcatccaaac agcattttta ttacacgggg tattgggaga ataatcaccg aaaaggggga
      661 gcactatggc aacggcagaa atttaccgtc cgaaacacca tattcgcttt gtaacggcat
      721 ccagcctatt cgatgggcat gacgcatcga tcaatatcat gcgcagaatc ctgcaggcaa
      781 gcggtgcgga agtcatccac ttaggacata accgttccgt cgaagaagtg gtgaacgctg
      841 caattcagga agacgtacag gggattgcca tttcatcata ccaaggcggt catgtggaat
      901 atttcaaata tatgtatgat ttacttcaag aaaaaggtgc accgcacatc cgaatttacg
      961 gaggtggcgg tggggtgatc atcccgaggg aaattaaaga actgcatgac tatggtattg
     1021 catggatttt ctcaccggaa gacggccgca agttggggct tcaaggtatg attaaccgga
     1081 tgctcgaaga atgtgatttt tcgacggcga aagaaaacgc agttgaactg gcaaatgcac
     1141 tatccacgga ccgtccagaa gtgttagcga acttaataac agtagcggaa gaagcacata
     1201 gttcaggggt ggactccgag actttactga ctcaagtacg tgcaaactcc aagaacacac
     1261 cagttatcgg gattacggga acgggcgggg caggaaaaag ctccttgact gatgagctga
     1321 tccgacgatt cttacaggag cttccggaga aaaaggtagc gatcctatca atcgacccaa
     1381 caaaacagaa aactggcggt gccctattag gtgaccgtat tcggatgaat gcaatcttca
     1441 acaaacgtgt attcatgcgc agtttagcaa cccgaggttc caaatcagaa ctttcaggtg
     1501 cgattaaaga cgtattggac gtcgtcaagg cagcaggtta tgatctgatt atcgtggaga
     1561 cgagtggaat cggccaaggg gatgctgaaa tcacaggtgt ttccgacgtt tccatgtacg
     1621 tgatgacaag cgaattcggg gcaccatctc aattagaaaa gatcgacatg atcgactatg
     1681 cagatttgat cgttatcaat aaattcgagc ggaaaggatc agaggatgct cttcgccaag
     1741 tgcaaaagca ataccagcga agccatctgc tgttcgacga atcgctcgac aagatgcctg
     1801 tgtacggcac aattgccagc cagttcaacg acaagggaac aaattcctta ttcgcagcgt
     1861 taatctctaa gctgaatgaa aaatgcggaa cggactgggc aacaacttat gaacagttcg
     1921 taaaaacgca aaagcaggac gtaatcattc cgaatgaccg tcgtcattat ttaagggaaa
     1981 ttacggacac agtccgcggt tatcataaaa aatcagagca gcaagttgaa tttgcacgcc
     2041 gtttattcca attggaaggt gcgttggaag ctgtcaagga gacatctcct gacgatgctt
     2101 taatccaatc acttcaatca ttggcagacg gagtccggaa tgaattgtct gctgaatcaa
     2161 aacgaatcct ggaaaactgg gaaaagctga aagaagcata ctccggggac gaatttgtta
     2221 cgaaaattcg cgataaagaa atccgcacaa tcttacgcac tgaaagttta tcaggcttaa
     2281 agattcctaa ggtaatactg ccaaagttca aggactatgg tgaaattctc cgttgggttt
     2341 acaaggaaaa tgtaccggga tccttcccgt atacagcagg cgtatttcca tttaaacgcc
     2401 aaggggaaga cccgaaacgt caatttgccg gtgaaggtac accagagcga acgaaccgcc
     2461 gtttccacta cttatcgaag gacgatgatg ctaaacgtct atccactgca ttcgactccg
     2521 ttacacttta cggggaagat ccggattacc gcccagatat ttacgggaaa gtgggagagt
     2581 cgggtgttag catttgtacg ctcgatgata tgaagaaact gtatgacggc ttcgacctat
     2641 gtgctccgtc tacatccgtt tcaatgacca taaatggacc tgcgccgatc atcttggcga
     2701 tgttcatgaa tacggcgatc gatcagcaag ttcggatacg agaagaagaa gttgggcgtc
     2761 cgctaacttt ggaagaattc acagtggtac gcgatcagac gctacaagta gtgcgcggca
     2821 ccgtacaagc tgacattcta aaagaggacc aaggccaaaa tacttgcatc ttctcgacag
     2881 aattcgccct tcggatgatg ggggatattc agcagtattt catcgaccac aaagtccgga
     2941 actattactc ggtctcaatt tccggctacc atattgcaga agctggggct aacccgattt
     3001 ctcagttggc gtttacgcta tcgaacggct tcacatacgt cgagtattat ttaagccgag
     3061 gcatgaatat cgatgatttt gctccgaact tgtcgttctt cttctcgaac ggacttgacc
     3121 ctgaatacac ggtcatcgga cgcgtagcac gccgcatttg ggcagttgcg atgcgtgaga
     3181 aatatggtgc caatgaacga agccaaaagc tgaagtacca tgttcaaaca tctggacgtt
     3241 ctcttcacgc gcaagaaatc gacttcaatg atatccggac tacgctccaa gcattgatgg
     3301 cattgcagga taactgtaac tcgcttcata cgaatgctta cgatgaagcg
//
LOCUS       DASULI010000448         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1271469,
            whole genome shotgun sequence.
ACCESSION   DASULI010000448 DASULI010000000
VERSION     DASULI010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271469"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VFX34_09680"
     CDS             <1..965
                     /locus_tag="VFX34_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006210032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5565222.1"
                     /translation="KADDVDFFDKLLRDHIISNFKDEETEREAFLETVKTVRGQINAA
                     LHIFEDDDASFVEEPIIDAANYSLQGDGKRLRPIVTWIMGVHEYGLDQSAIMPLLKSL
                     EYMHTASLIFDDLPSQDDSSVRRGRPTVHEVYNTAVAELTGLYLTQKAVEEQASLHRF
                     DSKDVLRLIQYSAGKTADMCKGQAMDLNSKGKQLTLDQLNAICFYKTGIGFEASIVMP
                     AILAGKQEEELTALKTFAYHAGIAFQIKDDLLDVEGNLASLGKPTGKDVENNNSTFVS
                     VLGTEGARKAMWDHYCTAMETLQEMHHKTTYLKHLLNYIVNRDS"
     gene            complement(1198..>2191)
                     /locus_tag="VFX34_09685"
     CDS             complement(1198..>2191)
                     /locus_tag="VFX34_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5565223.1"
                     /translation="GLLLTGLVYEQASRRRDSRRPYPGKMIDVGGYRLHITDAGQDAP
                     TVVIIHGAGDCSYSWMYIRKELSKFTRVITYDRAGMGSSDPGPEPTPEHTVKELHSLL
                     NMTGARGPYVLVGHSLGGLIARLYALKYPNQVAGLVFLDSTHESLKDDAKFKQGFAVI
                     GFMLKILRLASPFGIPRFLGNVLGVIPMFANELPYYKQQLNTEEYKQWKGNVYSIFAG
                     RTAGLEFKGAWPHLDAAADHLQNSSENPQFGDLPIAVVNNPGFGAQWTEMQKELASRS
                     SNSIHKISDRKGHSLQMPRPEYVIEAIEHVVDQVQERNSRTLAESRQEPVLELK"
BASE COUNT          608 a          508 c          515 g          560 t
ORIGIN      
        1 gaaaagcgga tgatgtcgat ttcttcgata aactgctacg ggaccatatc atttccaatt
       61 tcaaagatga agagacagag cgggaagcct ttttagaaac ggtcaaaacc gttcgtggtc
      121 aaataaatgc cgccttacat atcttcgaag acgatgacgc ttctttcgtc gaagaaccga
      181 tcattgatgc tgctaattac agtctgcaag gcgatgggaa aaggctcagg ccgatcgtga
      241 cgtggatcat gggcgttcac gaatatggac tggatcagtc tgcaatcatg cccttattga
      301 aatcattgga atatatgcat actgcatcgt tgatcttcga tgatttgccg tcccaggatg
      361 attcgtcggt ccgaagggga cgcccgacag tgcacgaagt gtataatacg gcggtggcgg
      421 agttgaccgg cctctatctg acgcaaaaag cggtggagga acaggcatca ctacaccggt
      481 ttgattcaaa ggatgtcctt cgcttgatcc aatattcagc tggaaagaca gcggacatgt
      541 gcaagggtca agcaatggac ttgaattcca aaggcaaaca attgacgctg gatcaattaa
      601 atgcaatctg cttctataaa accggcatcg gcttcgaagc ctccatcgtc atgcccgcca
      661 tcctagcagg gaagcaggaa gaggagctga cggctttaaa gacttttgcc taccacgccg
      721 gcatcgcctt tcagatcaag gatgacttgc ttgacgttga aggcaatctg gcatcactcg
      781 gaaagccaac cggaaaagac gtcgaaaaca acaactcgac tttcgtctca gttctaggta
      841 ctgaaggcgc ccgaaaagca atgtgggacc actactgcac cgcaatggaa acgcttcaag
      901 aaatgcacca caaaacaacc tacttgaaac acctcctcaa ctacatcgta aatcgagata
      961 gttgataagt tgtcagaatc tttccggtgc ctgtgcagca aactattcag actatgcaat
     1021 acaattgtat atagaggata agtgggtact gtagtcgttg gatttcattg tccgcatagt
     1081 gtgttattat tcgtcaagtt gaattatcat aaatatacct tgcacaggca cccacacaat
     1141 aggaaaagcc tcgttcaatt cgatgcagga gcatcaaaat tgaacgaggc tttttgatta
     1201 cttcaactca agtacgggtt cttgacgtga ttcagcaagg gttcgactgt tccgttcctg
     1261 gacttgatca acaacgtgtt cgatcgcttc aattacatat tcgggccgcg gcatttggag
     1321 actatgcccc ttacgatcgc ttatcttatg gatactgtta gatgaccggg aggcaagctc
     1381 cttttgcatc tcggtccatt gagcaccaaa tccgggatta ttgacaacgg cgatcggaag
     1441 gtcaccgaac tgggggtttt ccgaactgtt ttgtaagtga tccgcagcag catccaagtg
     1501 tggccaggca cctttaaact ccaaaccggc cgtccttccg gcaaagatac tgtaaacatt
     1561 acctttccat tgcttatatt cttcggtatt caactgttgt ttgtaatagg gaagttcatt
     1621 tgcgaacatc ggaatgacgc ctagaacatt accgaggaat cggggaatcc caaatggaga
     1681 ggctagcctt aaaatcttaa gcataaaacc gatgacggca aaaccttgct tgaactttgc
     1741 atcatccttg agtgactcat gcgttgaatc caggaacaca agtccagcta cctgattcgg
     1801 atatttcaaa gcatatagtc tggcaatcag tccccctaag gaatgtccga caagtacata
     1861 aggtccacgg gctccggtca tatttagcag gctatgtagt tctttgacgg tatgctcggg
     1921 ggttggctcg gggccagggt cacttgaacc catgcctgca cggtcatatg taatgactct
     1981 tgtaaacttg gataattctt tgcgaatata catccatgaa taagagcaat cgcccgcacc
     2041 atgaatgatg acgactgtag gtgcatcttg gccggcatca gttatatgga ggcgatagcc
     2101 accgacatct atcatttttc ccggataagg tcttctcgaa tccctgcgcc gtgaagcttg
     2161 ttcatatacg agtccggtta gaagtaaacc t
//
LOCUS       DASULI010000449         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1454619,
            whole genome shotgun sequence.
ACCESSION   DASULI010000449 DASULI010000000
VERSION     DASULI010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454619"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFX34_09690"
     CDS             complement(<1..294)
                     /locus_tag="VFX34_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012292615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5565224.1"
                     /translation="MVRYRFETDEHVLFRETLRKFLAKEAVPHYEQWEKDRLIPVSFW
                     RKLGEMGFLCPQVEEQYSGLGLDFSYGVIIGEEMERVGAGLTGVGLHNDIVVPY"
     gene            complement(310..1080)
                     /locus_tag="VFX34_09695"
     CDS             complement(310..1080)
                     /locus_tag="VFX34_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5565225.1"
                     /translation="MEMQEIIAIVTGGASGLGEATTRRIVAAGGKVAVFDMNEERGKA
                     LVDELGEDHVLFLKTDVAKTENVEANVAEAVSVFGKLNAVVNCAGIATPGKVVSKGGP
                     MVLDRFEQVIRVNLTGTFNVIRVAAAAMMSNEPNVEGERGVIVNTASVAAFEGQIGQA
                     AYSASKGGVVGMTLPIAREFAGYGIRVMTIAPGLVETPLFAGLPEPARNALEELTLFP
                     KRLGRSEEYARLVESIFTNPLLNGETIRLDGAIRMQAK"
     gene            complement(1116..2264)
                     /locus_tag="VFX34_09700"
     CDS             complement(1116..2264)
                     /locus_tag="VFX34_09700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5565226.1"
                     /translation="MREVVIVEGVRTAVARRKGGFSEYRPDELAAVVLEELVKRAGIE
                     KGDIEDVILGCVTQSGEQGGNIARTAALIAGFPVHVPGVTIDRQCGSSQQAVHFASQA
                     IASGDMDIVIAGGVESMTRAPMFSNMGDAKPSPKLMEKHEIINQGLSAERIAEKWGLS
                     REELDRFAYESHQKAFRAIEEGKFDDEIVPVAVNRVDGEVETVAKDEGPRPDSTPEVL
                     AGLKTVFDENGVITAGNASQMSDGASAVLLMSKEKAEELGLKPLARIVARTVVGSDPT
                     LMLTGPIEATKKVLLKAGLSIEDMDVYEVNEAFAPVPLAWLKDTGADPEKLNRNGGAI
                     ALGHPLGATGTKLLVSMMKELQRTGGRYGLLAICEGMGMANATIIERI"
     gene            2464..2817
                     /locus_tag="VFX34_09705"
     CDS             2464..2817
                     /locus_tag="VFX34_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5565227.1"
                     /translation="MKKFGLTILGIVAAIVVLANLGSILALAISGAIAFAGFHYYRQS
                     DSTFLKLFWGIVLLIGLVTAVSNVPAFIGIIALLGVYYVWRKWNERTNDNVIEAKYTD
                     DPFVNFERQWDEITK"
     gene            2831..>3165
                     /locus_tag="VFX34_09710"
     CDS             2831..>3165
                     /locus_tag="VFX34_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX5565228.1"
                     /translation="MNTLWNRIKYTVQADLHSALDKKESKNPIAMLNQYIKEAEKQTE
                     SVGKLLERQGKLKAELEKEFAEAEVMLEKRRRQLELAQSTGEEDLVAFTQDEILAYET
                     RANELAASIT"
BASE COUNT          735 a          913 c          738 g          779 t
ORIGIN      
        1 gtacggaacg acgatgtcat tatgcagtcc gacccccgtg agtcctgccc ccacccgctc
       61 catctcctcg cctataatga cgccgtagct gaagtcgagg ccaagcccgc tgtattgctc
      121 ttccacttgc ggacaaagga atcccatttc tccgagcttc cgccaaaagc tgaccggaat
      181 gagccggtct ttctcccatt gttcgtaatg gggaaccgct tccttcgcta aaaacttccg
      241 aagcgtctcc ctgaatagga catgttcatc tgtttcaaat cgatagcgca ccattcgatt
      301 cccccccttt tatttcgcct gcatccggat tgccccgtcc aaccggatcg tttctccgtt
      361 cagcagcgga ttcgtaaaga tgctttcgac aagccgcgcg tactcttcag atcgaccgag
      421 tcgtttaggg aataacgtca actcctcaag cgcattgcgt gcaggctccg gcaatcccgc
      481 gaagagcggc gtttccacga ggccaggcgc gatggtcatg acacggatgc cgtaaccggc
      541 aaattcgcgt gcaatcggta atgtcattcc gacgactccg ccttttgatg cgctgtatgc
      601 cgcctgcccg atctgccctt cgaatgccgc cacggaagcg gtattcacga tgacgccccg
      661 ctcaccttcc acattcggct cattgctcat cattgcagca gccgccaccc ggatgacatt
      721 gaacgtgccc gtcaaattga cgcggatcac ttgctcaaaa cggtccagca ccatcggacc
      781 gcctttcgac acgactttcc ctggcgtcgc aatgccagcg cagttgacga cggcattcaa
      841 ctttccgaac accgatacgg cttctgccac attcgcttcg acattttcgg ttttcgccac
      901 atccgttttc aggaataaaa catggtcttc cccaagctcg tcgactaatg ccttgccacg
      961 ttcttcattc atatcgaaaa cagcgacttt accgcctgca gccacgattc gacgagtcgt
     1021 cgcctcccct agaccggaag cccctccggt gacaatcgct atgatttctt gcatttccat
     1081 ttgcccatcc ccctctatgt aattgctaaa tcaagtcaaa ttctttctat aatagtagcg
     1141 ttcgccatgc ccatcccttc gcaaatagcg agcagtccgt aacgtccacc cgtacgctgc
     1201 agctccttca tcatcgacac gagcagtttc gttcctgttg caccgagcgg atgaccgagt
     1261 gcaatcgccc cgccgttccg gttgagcttc tctggatccg cgcccgtgtc ctttaaccag
     1321 gcgagcggaa cgggagcgaa tgcttcattc acttcataca cgtccatatc ttcaatggaa
     1381 agtcccgcct tcaaaagcac ctttttagtc gcttcaatcg gaccggtcaa cattaacgta
     1441 ggatcggagc cgacaaccgt acgggcgacg atccgcgcaa gcggcttcaa tccgagttcc
     1501 tccgcctttt ccttcgacat gagaaggacc gcggaagcac cgtcgctcat ttggctcgca
     1561 tttcctgcgg tgatgacgcc attttcatcg aacactgttt tcaggccggc aagcacttca
     1621 ggtgttgaat ccggacgtgg gccttcatcc ttcgcaacgg tttccacttc accatcgacg
     1681 cgattcacag caaccggcac gatttcgtca tcgaatttgc cctcttcaat ggcccggaac
     1741 gctttctgat ggctttcata tgcaaaacga tcgagttctt cacgggaaag tccccatttt
     1801 tccgcaatac gctctgcgga tagtccttga ttgatgatct catgcttttc catcagcttc
     1861 gggctcggtt tggcatcccc catattcgag aacatcggcg ccctcgtcat gctttccacg
     1921 ccgccagcaa tgacgatgtc catatcccct gacgcaatcg cctgcgaagc gaagtggacc
     1981 gcctgttggc tcgacccgca ttgccgatcg attgtgacgc ccggcacatg gaccggaaag
     2041 cctgcaatca acgctgcagt ccgcgcaata ttgccccctt gttcacccga ctgcgtgaca
     2101 cagcctaaaa tgacgtcctc tatatcacct ttttcgattc ccgcgcgttt caccagttct
     2161 tcaaggacga cagccgcaag ctcgtccgga cgatattccg aaaaaccgcc ttttcgcctt
     2221 gcgactgcgg tccgtacccc ttcgacaatg acaacttccc tcatatcggc acctcattct
     2281 ctgaattaat tgatagttca tatagttata gcttacagga aagcgctacc aatatctaga
     2341 gtattttagg tgtacctagt ccggaatcgg tctcaagtcg tagatggaaa tccgctttgc
     2401 gcctgtttgg atgaatcgat gcgcattgta agatgtagat acaacgaaag gaggaacaac
     2461 aatatgaaaa aatttggtct aacgattctc ggcatagtgg cggcgatcgt tgttctcgca
     2521 aacttagggt cgattttggc acttgccatt tccggtgcaa tcgcttttgc agggttccat
     2581 tattacaggc agagtgattc tacattcttg aagctgttct gggggatcgt cctgctgatc
     2641 ggtttggtga cagccgtttc gaacgtgcct gcattcatcg gcatcattgc attgctcggt
     2701 gtctactatg tatggcgtaa atggaatgaa cgcacaaacg acaatgtgat cgaagcgaag
     2761 tataccgacg acccgttcgt caacttcgaa cgacaatggg atgaaattac aaaataagga
     2821 ggaaacgatg atgaacacgt tatggaatag aataaagtat acggtgcaag ccgatttgca
     2881 ttcagcgctc gataaaaagg agtcgaaaaa tccgattgcg atgttgaacc agtacatcaa
     2941 agaagcggag aaacagacgg aatcggtcgg caagctgttg gagcgtcaag ggaagttgaa
     3001 ggctgagctt gagaaggagt tcgcggaagc ggaagtgatg cttgaaaaac gccgccgtca
     3061 attggagctc gctcaatcga caggcgaaga ggatttggtc gcgttcactc aggatgaaat
     3121 ccttgcttat gagacacgtg caaacgaatt ggcggctagc atcac
//
LOCUS       DASULI010000450         8112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1818386,
            whole genome shotgun sequence.
ACCESSION   DASULI010000450 DASULI010000000
VERSION     DASULI010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818386"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFX34_09715"
     CDS             complement(<1..736)
                     /locus_tag="VFX34_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEX5565229.1"
                     /translation="MQFKDIGVLAAVIMIVAMLVIPLPPWLLSFLIIINITLALLVLL
                     TSMNMQEALQFSIFPSLLLLLTLFRLGLNVSTTRAILSQGDAGGVVDTFGTFVTGGNI
                     VVGLVVFVILIIIQFIVITKGSERVSEVAARFTLDAMPGKQMSIDADLNAGMISETEA
                     RMRREKVSNEADFYGAMDGATKFVKGDAIAGIIIVIINLLVGMIIGVAQMDLPFAEAA
                     TKFSKLTVGDGIVSQIPALLISTATGI"
     gene            complement(774..1856)
                     /gene="flhB"
                     /locus_tag="VFX34_09720"
     CDS             complement(774..1856)
                     /gene="flhB"
                     /locus_tag="VFX34_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEX5565230.1"
                     /translation="MIRLDLQFFAGEKTEKATPKKRLDSRKKGQVLKSQDVTTAIVLL
                     FVFLFLFFAAGFMRDRFFVFFTHSFTESISIKATLDIEQTMILYTDMIVQMAYILLPV
                     MAIAVIAGIVGNLMQFGLLFTAEPLKFDLKKIDPIKGLKRIFSIRALVELLKSLLKIS
                     FIGSVTTFLLMTNIEKVLGLAFKTPHDTLVTVGQLVALMGIVASFVLLFISVLDYFYQ
                     KFDYEKNLRMSKQDIKDEYKNTEGDPIIKSRIKQRQREMAMRRMMQEVPHADVVITNP
                     THFAIALKYDDEKMDAPVVIAKGADFIAQKIKLIAKEHDIVMVENRPLARALYDDVEI
                     GDRIPDEFFKTVAEILAYVYRIQRKI"
     gene            complement(1860..2636)
                     /gene="fliR"
                     /locus_tag="VFX34_09725"
     CDS             complement(1860..2636)
                     /gene="fliR"
                     /locus_tag="VFX34_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX5565231.1"
                     /translation="MEELIPSLAVFLLILTRVTAFIVTVPLFSYRAIPTTQRIIFAAL
                     LAWMMTYSIEAPELDIDGKYILLVVKEATTGLFIGIIAYIVMSAIQIAGGFIDFQMGF
                     AIANVIDPQTGAQSPLLGQFFNALAILLLLTLNGHHMLLDGIFYSYRFIPIDMLWPAF
                     GDERLIEFVIRTFAVVFAIAFQMAIPIVATLFLVDLALGITARTVPQLNIFVVGFPIK
                     IGVSFLVLVTMFTVMIAMMRKLFEVMISAMRDLMIILGGG"
     gene            complement(2641..2910)
                     /gene="fliQ"
                     /locus_tag="VFX34_09730"
     CDS             complement(2641..2910)
                     /gene="fliQ"
                     /locus_tag="VFX34_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX5565232.1"
                     /translation="MTGELVIAIAERAVWVILLTCGPLLIVALVSGLVVSIFQATTQI
                     QEQTLAFVPKIVAVLVAIVFFGPWMLARVTAFATDIFDNLVRYIG"
     gene            complement(2975..3640)
                     /gene="fliP"
                     /locus_tag="VFX34_09735"
     CDS             complement(2975..3640)
                     /gene="fliP"
                     /locus_tag="VFX34_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293275.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX5565233.1"
                     /translation="MNDFVQFFSDSNPTNVSTSIKMLLLLTVLSLAPAILILMTSFAR
                     IVIVLSFVRTALATQQMPPNQVIVGLALFLTFFVMAPTFQQVNENALTPLFAEEITLE
                     EAYDQASMPFKEFMSKHTRQKDLELFLRYNQAERPETLEDIPLTMLVPAFALSEIKTA
                     FQMGFMIFIPFLVIDMIVASVLMSMGMMMLPPVMISLPFKILLFVLVDGWYLIVKSLL
                     QSF"
     gene            complement(3633..4325)
                     /locus_tag="VFX34_09740"
     CDS             complement(3633..4325)
                     /locus_tag="VFX34_09740"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX5565234.1"
                     /translation="MNLSFLQKFLSIFLLTIFLIGSFPFLSHADDNTDKSVSDWFGKE
                     DDSEQDIPKVIDENEPAADRSKDGAAVGLSGWDYVKTLLALLFVVGLLFALLKFINRK
                     NRMFDQHRLMKNVGGLSLGQQKSIQLVVIGETYYLIGVGDEVRLLKEITDPDEIKALE
                     SYFDEDELQSTPGLANKLLTLLPLGKGDASDESEQPADFKKMFTSRLDELKAERKKHL
                     NRLAEKESRKDE"
     gene            complement(4329..4691)
                     /locus_tag="VFX34_09745"
     CDS             complement(4329..4691)
                     /locus_tag="VFX34_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5565235.1"
                     /translation="MGKRILIVDDAAFMRMMIKDILTKNNFEVVGEAADGAQAVEKYM
                     ELKPDLVTMDITMPEMDGIAALKSIKEKDPSATIIMCSAMGQQAMVIDAIQAGAKDFI
                     VKPFQADRVVEAIQKAIG"
     gene            complement(4711..5940)
                     /gene="fliY"
                     /locus_tag="VFX34_09750"
     CDS             complement(4711..5940)
                     /gene="fliY"
                     /locus_tag="VFX34_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HEX5565236.1"
                     /translation="MSDNVLSQEEIEALLRGEPIRAEETATSSSEHQDFDNQLSEMEK
                     DALGEVGNISFGSSATALSSLLGQKVEITTPAISVVDRSMLETEFIHPYVAVQVEYTE
                     GLNGTNLLVIKQSDAAIIADLMLGGDGLAPNEELNEIHLSAVQEAMNQMMGSAATSMS
                     TVFNKKVDISPPSIDLMNMQNEEGAEKIPKDEMMIKVSFELKVGNLIDSNIMQLFPLQ
                     FGKNLVASLMGMGAEEEAAVTLEAPTRPAPRSEQQPKRQEFPQEDLSDFDKGQSASQS
                     IGHPTYEQPYYEQPVQQQMPQRQQQPPVHVQQARFASFEGPSLKQAETNNLSMLLDIP
                     LQVTVELGRTKRSVKEILEMSSGSIIELDKLAGEPVDILVNNRHIAKGEVVVIDENFG
                     VRITDILSQTERLNNLR"
     gene            complement(5933..6931)
                     /gene="fliM"
                     /locus_tag="VFX34_09755"
     CDS             complement(5933..6931)
                     /gene="fliM"
                     /locus_tag="VFX34_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009330749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX5565237.1"
                     /translation="MPGDILSQNEIDALLSALSTGEMSAEEMKKEEETRKVKVYDFKR
                     ALRFSKDQIRSLTRIHENFARLLTTYFSAQLRTYIQINVASVDQIPFEEFIRSIPNMT
                     LLNIFEVPPLEGNIIMEVNPNVAYSMLDRLMGGVGEGPGKVDNMTEIETKILTNLFER
                     SFDNLREAWSGIIEMDPFLSEMEVNPQFLQMISPNETVVVISFNIVIGESSGMINICI
                     PHVVLEPIVPNLSVRYWMQTNKKEPTPEQSVILEKRLRQATLPLSTELGKGAMTVEDF
                     LHLQVGDVISLDRKIDEPLIVKVGDIPKFSAQPGHLRNRMAVQIIDILNGGDEDDE"
     gene            complement(6969..7394)
                     /gene="fliL"
                     /locus_tag="VFX34_09760"
     CDS             complement(6969..7394)
                     /gene="fliL"
                     /locus_tag="VFX34_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HEX5565238.1"
                     /translation="MKNKALTIALIIVVTLTLVGIIGLILTLQLKGDSASKEPTIDDI
                     LEASVDVPEIMTNLSGRQFIRISLKIQTTDKKAAAELAKRDFQVQNIVIQELSEMTSK
                     DFEGKTGKQAFEDSIKSQLNPLMQEGEIEKVYIVSYIIQ"
     gene            complement(7384..7605)
                     /locus_tag="VFX34_09765"
     CDS             complement(7384..7605)
                     /locus_tag="VFX34_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HEX5565239.1"
                     /translation="MIKVTRLNGKAFMLNALYIETVESFPDTTITLTTGTKYVVLDPI
                     DEVNRRIIEFYQSVQLLSNPHIRGEENEK"
     gene            complement(7919..>8112)
                     /locus_tag="VFX34_09770"
     CDS             complement(7919..>8112)
                     /locus_tag="VFX34_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX5565240.1"
                     /translation="APVHDFATDGGIGSIESGFLEMSNVDLAEEFTEMIVAQRGFQAN
                     TRIITTSDEILQELVNLKR"
BASE COUNT         2183 a         1914 c         1702 g         2313 t
ORIGIN      
        1 caatacctgt cgcggttgaa atgagcaaag ccggtatttg ggaaacgatt ccatccccga
       61 cggtcaattt ggagaatttg gttgcagcct cggcaaatgg caagtccatc tgcgcaacac
      121 caatgatcat ccctacaaga aggttgatga tgacgatgat gattccagcg atggcatctc
      181 ctttaacaaa tttcgttgct ccatccatcg caccatagaa gtcggcttca ttgctcacct
      241 tttcacggcg catccttgct tccgtctcag aaatcattcc tgcgttcaaa tcggcatcga
      301 tactcatctg cttacctggc atcgcatcga gtgtgaaacg ggcagcaact tcagaaaccc
      361 gctcagaacc tttcgtgatg acgatgaatt gaatgataat caaaatgaca aacacgacaa
      421 gaccgacgac gatattgcca ccggttacaa aagtaccgaa tgtgtcgaca accccgcctg
      481 catccccttg tgaaagaatt gcacgggttg ttgagacgtt caatcctagc cggaatagcg
      541 tcaacaataa aaggagtgaa gggaaaatcg aaaattgcaa ggcttcctgc atattcattg
      601 aagtcaaaag gaccaatagg gcaagtgtaa tattgatgat gatcaaaaaa ctaagaagcc
      661 agggcggtaa tgggatgacg agcatggcaa caatcataat gaccgctgcc aatactccta
      721 tatctttaaa ctgcatgcca tcccctcttt ctctatatgt atatcaatct gagttatatt
      781 ttccgttgta tgcgataaac atatgccaat atttcagcga ctgtcttgaa aaactcgtcc
      841 ggaatgcgat cgccgatttc aacatcatcg taaagcgccc ttgccagcgg gcgattttcc
      901 accatgacga tatcatgttc tttcgcaatc agcttgatct tttgggcgat gaagtctgcc
      961 cccttcgcga taacgacggg tgcatccatc ttttcatcgt catatttcag ggcgattgca
     1021 aagtgggtcg gattcgtgat gacgacatcc gcatgaggca cttcctgcat catccttcgc
     1081 atcgccattt ccctttggcg ttgtttgatg cgcgacttga tgatcggatc gccttccgta
     1141 tttttatatt catctttaat atcttgtttt gacatcctca agtttttctc atagtcgaat
     1201 ttttgataaa aatagtccag aactgaaatg aacaagagga cgaatgatgc gacaatcccc
     1261 attaaagcta ccaattggcc aaccgtgacc aatgtatcgt gcggtgtctt gaaagcaaga
     1321 cctaacactt tttcaatatt cgtcatgaga aggaacgtcg tcactgaacc gataaatgaa
     1381 atcttcaaca aggatttcaa caattcaacg agcgcccgta tggagaatat ccgtttcaat
     1441 cctttgatcg gatctatttt cttcagatcg aatttcaaag gttcggcagt aaaaagcaaa
     1501 ccgaattgca tcaaattccc cacaatgccg gcgattacgg caatcgccat gaccggcaaa
     1561 agtatgtacg ccatttggac aatcatatct gtgtaaagga tcatcgtttg ttcaatatcc
     1621 aatgttgcct ttattgagat ggattctgta aaagaatgcg taaagaaaac aaaaaatcgg
     1681 tcccgcatga atcccgcagc gaaaaaaagg aacaggaata cgaagagaag gacaattgca
     1741 gtcgttacat cctggctctt taatacctgt cctttctttc gggaatcaag tcgtttcttc
     1801 ggagtggctt tttccgtctt ttcgcctgca aagaactgta agtccaaacg aatcattctt
     1861 cagccacctc ctaagatgat catcaaatcc cgcatcgccg aaatcatcac ttcaaaaagc
     1921 ttccgcatca ttgcgatcat gaccgtgaac atcgtcacaa gaacgaggaa actgaccccg
     1981 attttaatag gaaacccgac aacaaaaata ttcagttgag gaaccgtcct cgctgtaata
     2041 cctagcgcca aatcaacaag aaacagggtg gcgacaattg gtatggccat ctggaaggcg
     2101 atggcgaata caaccgcaaa tgtcctgatg acgaattcga ttagccgttc atcgccgaaa
     2161 gctggccaaa gcatgtcaat cggtatgaat cggtaactgt aaaaaatgcc atcgagcagc
     2221 atatgatgtc cattcaatgt caataacagc aaaatcgcca gcgcattgaa aaattggcca
     2281 agcaatggtg attgggcacc tgtttgtggg tcgatgacgt tcgcaatggc aaaacccatc
     2341 tggaagtcga tgaatccacc ggcaatttgg attgccgaca tgacgatata ggcgataata
     2401 ccgataaata atccggtcgt tgcttccttg accacaagga gtatgtattt gccgtcgatg
     2461 tcaagttcgg gtgcttcaat cgaataggtc atcatccaag ccaatagtgc agcaaagatg
     2521 atcctttgcg tcgtcgggat tgcccggtag gaaaataatg gaacagttac gataaaagcg
     2581 gtcactcttg tcaaaatgag caagaagacg gctaaagaag gaatcagttc ttccattcca
     2641 tcacccgata taccgaacta gattatcgaa aatatctgta gcgaatgctg taaccctcgc
     2701 caacatccaa ggtccaaaaa atacaatggc aacaaggact gcaacaatct tcggcacaaa
     2761 tgccaatgtt tgttcttgga tctgcgtcgt tgcctggaat atgctgacga ccaaaccgga
     2821 caccaatgcg acaatcaaca gcggcccgca agttaataaa atgacccaaa ccgcacgttc
     2881 agctatcgct atgactaatt cgcctgtcat tactaaccac tcctaaactg taaaattgcc
     2941 gattttccga tagggatttg acaggtgatt gcagctaaaa actttgcaat agagatttga
     3001 cgatgagata ccatccatcg acaaggacaa ataataatat tttaaatggc aatgaaatca
     3061 tgaccggcgg caacatcatc atccccatgg acatcagtac acttgcaaca atcatgtcga
     3121 tgactagaaa cgggatgaaa atcatgaacc ccatttggaa tgcggtcttt atttcactca
     3181 gcgcaaaagc aggcactaac attgtcaacg gaatgtcttc caacgtttcg ggacgttccg
     3241 cttgattgta tcgtaaaaat aattccagat ccttctgtct cgtatgtttg ctcatgaact
     3301 ctttgaacgg catactagcc tgatcgtatg cttcctccaa tgtgatttct tcagcaaaaa
     3361 gaggtgtgag agcattttca ttcacctgtt ggaaggtcgg tgccataaca aagaatgtca
     3421 aaaacaaagc aagaccgact atgacttggt ttggcggcat ttgctgcgtt gccaacgcgg
     3481 ttcttacaaa agataacacg atgacaatcc ttgcaaacga tgtcataaga ataagaattg
     3541 ccggcgcaag cgataaaaca gttagaagga gaagcatttt gatggatgtc gatacgtttg
     3601 tcgggttgct gtccgagaaa aactggacga aatcattcat cttttctgct ctccttttct
     3661 gccaatcggt tcaaatgctt ttttctttcc gctttcaatt catctaatct ggatgtgaac
     3721 atctttttga aatcggcggg ttgttcgctc tcatccgacg cgtccccttt acccagcgga
     3781 agcagcgtca gcaatttatt cgcaaggcct ggcgtcgatt gaagctcatc ttcatcgaaa
     3841 taactttcca aagccttgat ttcatccgga tcggtaatct ctttcaataa ccttacttca
     3901 tctcctacac caatcagata gtaggtttct ccgatgacga ccaattgaat ggatttttgc
     3961 tgccctaatg aaagtccgcc gacatttttc atcaatctat gttgatcgaa cattcggttc
     4021 ttccggttaa tgaatttcaa taacgcgaat agaagtccga ctacgaacag caatgccaat
     4081 agcgtcttta cataatccca tccagacaag ccgacagctg ccccgtcttt cgagcggtcg
     4141 gcagctggct cattttcatc gatcaccttt ggaatatctt gttcactgtc atcctctttt
     4201 ccaaaccaat cagatacgga tttgtccgta ttgtcatctg catgggacaa aaaaggaaat
     4261 gagccgatga ggaagattgt cagcaggaaa atcgatagaa acttttgtaa aaatgataaa
     4321 ttcataggtt aacctattgc tttttggatc gcttcaacaa cacgatcagc ttggaacggc
     4381 ttcacgatga agtctttagc acctgcttga attgcatcga tgaccatcgc ttgttgtccc
     4441 atggctgaac acatgatgat tgtcgctgaa gggtcttttt ctttaatgga tttcaacgcc
     4501 gcaatgccat ccatttcagg cattgtaata tccatcgtta caagatcggg tttcaactcc
     4561 atatattttt caacggcttg cgcaccatcg gcggcctctc cgaccacttc aaaattattc
     4621 tttgtcaaaa tatccttgat catcatacgc atgaaagccg catcatcaac tattaaaata
     4681 cgtttaccca ttctcctgac ctcccggatc ctatctcaaa ttatttaaac gttctgtctg
     4741 acttaatata tctgttatcc gcacgccgaa gttctcatcg atgacgacga cttccccttt
     4801 ggcgatatgg cggttattca ctaaaatatc aaccggttca ccggccagct tatccaattc
     4861 tatgatggaa ccactagaca tttcaagaat ttccttaacc gagcgttttg tccttccaag
     4921 ttcaacagtc acttgcaaag gtatatccag caacatactc aagttgttcg tttcggcttg
     4981 cttcaatgaa gggccttcaa agcttgcgaa acgggcttgc tgtacatgga ctggcggctg
     5041 ttgctgcctt tgtggcatct gttgctgaac cggctgttcg taatacggct gttcataagt
     5101 aggatgcccg atcgattgag aggcactttg ccctttatcg aaatcggaaa gatcctcttg
     5161 cggaaactcc tgccttttcg gttgctgttc actacgtgga gccggcctgg tcggtgcttc
     5221 caatgtaaca gcagcctcct cctctgcacc catccccata agcgatgcaa ccaggttttt
     5281 cccgaattga agtgggaata gctgcataat attggaatct atcaagtttc caactttcaa
     5341 ctcgaaggag actttgatca tcatttcatc cttcggaatc ttttcagcgc cttcttcgtt
     5401 ttgcatattc atcaagtcga tggatggcgg agatatatcg actttcttat tgaagaccgt
     5461 ggacatcgac gttgccgcag accccatcat ttgattcatc gcttcttgga ccgcacttag
     5521 atgaatttca ttcagttctt cattcggagc caatccgtct ccgcccagca taaggtctgc
     5581 aatgattgca gcgtcacttt gcttaatgac tagcagattg gtaccgttca atccttctgt
     5641 atattcaact tggaccgcta catatggatg aatgaattcc gtttccagca tactgcggtc
     5701 cacaactgaa attgcaggcg ttgtgatttc cactttttga ccaagcaatg aggagagggc
     5761 tgttgcggag ctgccgaagg aaatattccc gacttctcct aaggcgtctt tctccatttc
     5821 acttaattga ttgtcaaaat cctgatgttc agaggacgat gttgcagttt cttccgcgcg
     5881 aatcggttcg ccgcgcagca acgcttctat ctcttcctga gaaagtacat tatcactcat
     5941 catcttcatc ccctccattc aatatatcga ttatttgcac agccatcctg ttacgaagat
     6001 gaccaggctg tgccgagaat tttggaatgt ctcctacttt gacgattaac ggttcatcga
     6061 ttttccggtc aagggaaatg acatccccga cttgaagatg caagaagtcc tcaaccgtca
     6121 tggctccttt tcctaattcg gtagacagcg gcaatgtggc ttgtctcaat cgcttttcca
     6181 aaatgacact ttgttcaggt gtcggttctt ttttattcgt ctgcatccaa taacggaccg
     6241 ataagttcgg aacgatcggc tcgagaacga cgtgcggaat gcatatgtta atcatgccgc
     6301 tcgattcccc gatgacgatg ttgaatgaaa tgacaacaac tgtttcattc ggtgaaatca
     6361 tttgaaggaa ttgtggattc acttccattt cagataaaaa aggatccatt tctatgattc
     6421 ccgaccatgc ttcccgtaag ttatcgaatg accgttcgaa aaggtttgtt aagatcttcg
     6481 tttcgatttc agtcatattg tccacttttc cggggccttc ccctacgccg cccatcaatc
     6541 ggtcaagcat tgagtaggca acgttcggat tgacttccat gatgatattc ccttcaagcg
     6601 gcggcacttc gaaaatattg agcaatgtca tgttcggaat ggatcggatg aactcctcaa
     6661 atggaatttg atcgaccgaa gcgacattga tttggatata cgtccgcaat tgcgcggaaa
     6721 aataagtcgt caataatctt gcgaagtttt catggatccg cgtcaagctg cggatctggt
     6781 cttttgagaa gcggagggca cgtttgaaat catatacttt gaccttccgg gtctcctctt
     6841 ccttcttcat ttcttccgcc gacatttcac ctgtcgataa tgccgataat aaggcatcaa
     6901 tttcattttg ggataaaata tctcccggca taaggccacc tccccgcgta gtatatgtcc
     6961 atcgtttttt attggatgat ataggagacg atgtacactt tttcgatttc tccctcttgc
     7021 atgagcggat tcaattgaga tttgatggaa tcctcaaatg cctgcttccc tgtttttcct
     7081 tcaaaatctt ttgaggtcat ttctgagagt tcttgaatga cgatgttttg aacttggaag
     7141 tcccgcttcg ccagttcggc agcggctttt ttatcggttg tttggatttt caatgaaatc
     7201 cggataaatt gacgaccgga taagttcgtc atgatttcag gtacgtcgac ggatgcttca
     7261 aggatgtcat cgattgtcgg ttctttacta gcggaatccc ctttgagttg caaggtgagg
     7321 attaggccga tgatcccgac aagtgtcaag gtaactacaa tgataagtgc gattgtcagt
     7381 gctttatttt tcattttctt caccccggat atgcggattc gatagtagtt ggaccgactg
     7441 ataaaattca atgatgcggc gattgacttc gtcgatcggg tccaagacga cgtatttggt
     7501 tccggtcgtc agtgtgatgg tcgtatcggg aaatgattcg actgtttcta tgtatagggc
     7561 gttcaacatg aacgctttgc cattgaggcg tgtgaccttg atcatatgta aggggccggg
     7621 cacagggccg gcccgaccct cctttgctaa aaacgcatat atttctacgt cggattttcg
     7681 ctacaggcgg acgctttccg gggggcgggc ggtgagccaa tcgaaccacg aagagttcga
     7741 tttgccgtat ttctgcgctt tttgcagaaa ttaaggcatc cgttctacca ttcctgctcg
     7801 taacgcttcg cttttactcg caaacgccgt tcttcgttac ggcgttcgct aatcccccag
     7861 gagtcgccgc ctttcgctac aatcccaagt atgcaaaact atttattggt tcttagattt
     7921 atcgtttcaa gtttacaagt tcttgaagga tttcatctga tgttgtgata atacgagtat
     7981 ttgcttggaa accacgttgt gcgacgatca tttcagtgaa ttcctctgca aggtcgacgt
     8041 tggacatttc taggaaaccg gattctattg atcctatacc tccatcagtt gcaaagtcat
     8101 gaactggcgc ac
//
LOCUS       DASULI010000451         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1707292,
            whole genome shotgun sequence.
ACCESSION   DASULI010000451 DASULI010000000
VERSION     DASULI010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707292"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1512
                     /gene="tig"
                     /locus_tag="VFX34_09775"
     CDS             226..1512
                     /gene="tig"
                     /locus_tag="VFX34_09775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006830965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX5565241.1"
                     /translation="MSVKWEKEEGNKGTLTVEVPAEKVDAALDEAFKKVVKKVQAPGF
                     RKGKMPRKMFEKMYGVESLYNDALDIILPEAYSNALDEAEVEPIAQPEIEIDEMEKGK
                     ALVFKAHVTLKPEVKLGDYKGLEVTRQETEVTDEEVEQQLKDRQSALAEMVVKEDGAV
                     ENGDTVNLDFEGFVDGEAFEGGKAEGYDLEIGSGSFIPGFEEQVIGMKQGEEKDIDVS
                     FPEEYHAAELAGKPAVFKVKVHEIKSKEVPELDDELAKEIDESVESLEELRTKMKEQT
                     AEEKKSASETAMRDDLVEAAARNAEIDIPEVMIDSEVDRMMDEFGQRLQMQGMNLDLY
                     FQFSGQDEAALRSQMRDDALSRVRVSLTLEAIGAAENIKVTEEEVNAELEKMSEQFGM
                     EVDQIKQTLGGTKVLENDLRFNKTVEFLVENAKVAE"
     gene            1659..2927
                     /gene="clpX"
                     /locus_tag="VFX34_09780"
     CDS             1659..2927
                     /gene="clpX"
                     /locus_tag="VFX34_09780"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HEX5565242.1"
                     /translation="MFKFNDEKDNLSCSFCGKTQDQVRKLVAGQGVYICDECVELCAE
                     IVEEEVGLDEHFELKDVPKPREIQSILDEYIIGQDRAKKSLAVAVYNHYKRINTNSKV
                     DDVEIAKSNIVLIGPTGSGKTLLAQTLARILDVPFAIADATSLTEAGYVGEDVENILL
                     KLIQAADFDIERTEKGIIYIDEIDKVARKSENASITRDVSGEGVQQALLKILEGTVAS
                     VPPQGGRKHPHQEFLQIDTTNILFIVGGAFDGMEEIIKRRTGQKVIGFGTNTEEESKE
                     EASLLSKLIPEDLQRFGLIPEFIGRLPVIATLEQLNEDTLYQILTVPKNAIVKQYQKM
                     VELDGVTLRFEEDALLEIAKEAIERKTGARGLRSIIENIMLDIMYELPSLDEVTECII
                     TKDSVLKKSSPILYREDGTTMELDQEKNTA"
     gene            3048..>3252
                     /locus_tag="VFX34_09785"
     CDS             3048..>3252
                     /locus_tag="VFX34_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5565243.1"
                     /translation="MPNRKKLNVPLLPLRGMLVYPTMVLHIDVGRERSVFAVEHAIAN
                     GNYIMLASQKDTTVENPDAEDLYH"
BASE COUNT         1039 a          612 c          810 g          791 t
ORIGIN      
        1 cgaagttgcg agtgcgtata agcaatacat agaaagcatg ttcaccggag aaattgtgat
       61 cgcaacggaa ttgaattctt tgatcaaatc tgtcgatgaa caaacggata ggtaatatac
      121 agtaaatctg ttgaaagatc aaaagtctat gatatactgt aagggttgta aatagcgtat
      181 aaacgtgaga aaatatattg gtcattatga ttcggaggta tttacatgtc tgttaaatgg
      241 gaaaaagaag aaggcaataa aggaaccctc acagtagaag ttcctgctga aaaagtagat
      301 gcagcattgg acgaagcgtt caagaaagtt gtaaagaaag tccaagcgcc tggtttccgt
      361 aaagggaaaa tgcctcggaa aatgttcgaa aaaatgtacg gagtagaatc gctttataac
      421 gacgcgcttg acatcatcct tccagaagcg tattcaaacg cgctagacga agcggaagta
      481 gagccaattg ctcaacctga aatcgaaatc gatgaaatgg aaaaaggcaa agcgctcgta
      541 ttcaaagcac atgttacatt gaagccagaa gtgaaacttg gtgactataa aggtcttgaa
      601 gttacacgcc aggaaactga agtgacagac gaggaagtcg aacagcaatt gaaagatcgc
      661 caatccgcat tggctgaaat ggtcgtaaag gaagacggcg ctgttgaaaa cggcgacact
      721 gtcaaccttg acttcgaagg tttcgttgat ggtgaagctt ttgaaggcgg caaagctgaa
      781 gggtacgatc ttgaaatcgg ctcaggttca ttcattccag gattcgaaga gcaagtcatc
      841 ggaatgaaac aaggcgaaga aaaagacatc gacgtttctt tccctgaaga gtaccatgca
      901 gctgaattgg ctgggaagcc tgctgtgttc aaagtgaaag tgcatgaaat caagtcgaag
      961 gaagttcctg agcttgacga tgagcttgca aaggaaatcg atgaaagcgt tgagagcctt
     1021 gaagagcttc gcacaaaaat gaaagagcag acagctgaag aaaagaaaag cgcatctgaa
     1081 acagcaatgc gagatgatct tgtagaagct gctgcacgga acgcagaaat cgatatccct
     1141 gaagtgatga tcgattctga agtagatcgc atgatggatg aattcggcca acgccttcaa
     1201 atgcaaggca tgaaccttga cctgtacttc caattctcag gccaagatga agctgcatta
     1261 cgctcgcaaa tgagagatga tgcgcttagc cgcgttcgcg tttccttgac acttgaagca
     1321 atcggcgcag ctgagaacat caaagtgact gaagaggaag ttaatgcaga gcttgaaaaa
     1381 atgtccgaac aatttggtat ggaagtcgac caaatcaaac agactctagg cggaacgaaa
     1441 gtgcttgaaa acgacttgcg tttcaacaag actgttgaat tcctagttga aaatgcaaag
     1501 gtcgctgaat aataggtgat gaaaatacaa ggtgcgggtt tccgtgcctt gtttttcata
     1561 ggcggggatt ttttgggcaa ctatagagga taagttctaa atagttgata aaaccggggg
     1621 aatcttgtaa gattgtcact tgaatagggg tgagcttgat gttcaaattt aatgacgaaa
     1681 aagataatct aagttgttct ttttgcggta aaacccaaga tcaggtgcgg aagcttgttg
     1741 ccggtcaggg tgtttatatt tgtgatgagt gtgttgaact ttgtgcagaa atcgtcgagg
     1801 aggaagttgg ccttgatgag cacttcgagc tgaaggatgt tccgaaacca cgtgaaatcc
     1861 aaagcatatt agatgagtat attatcggcc aggatcgtgc gaagaaatca ttggctgttg
     1921 cagtttacaa ccactacaaa cggatcaata cgaacagcaa agtggacgat gtggagattg
     1981 caaaatcaaa tatcgttctg atcggtccta ccggtagcgg taaaacattg ctggcacaaa
     2041 cattggcgcg cattctcgat gttccgttcg caatagcgga tgcaacttct ttgacggaag
     2101 ccggttatgt cggtgaagac gttgaaaata tattattgaa actgatccaa gctgctgatt
     2161 tcgacatcga acgtaccgaa aaaggaatca tttatattga tgaaatcgat aaagttgccc
     2221 gtaaatcgga aaatgcatcg attacacgtg acgtatcagg tgaaggtgta cagcaagccc
     2281 ttttgaagat tcttgaagga actgttgcaa gcgtccctcc tcaaggcggc cgtaagcatc
     2341 cacaccagga attcctccaa atcgatacga caaacatcct tttcatcgtt ggcggcgcct
     2401 tcgatggcat ggaagaaatc atcaaacgcc gtaccggtca aaaggtgatc ggctttggca
     2461 caaacacaga agaggaatca aaagaggaag cttccttgct ttcaaaactg attcctgagg
     2521 atttgcagcg tttcggtctg attccagaat tcatcggccg tttgccggtg attgctacat
     2581 tggagcagtt gaacgaagat accctgtatc aaatcctaac ggtaccaaaa aacgcaatcg
     2641 tcaagcaata ccagaagatg gtcgaattgg atggtgtaac attgcgtttt gaagaggatg
     2701 cgcttttgga aatcgccaag gaagcgatcg aacggaagac gggtgctcgt ggattacggt
     2761 ccattatcga aaacatcatg ttggatatca tgtatgaact tccttcactt gatgaagtga
     2821 ctgaatgcat cattacgaag gattccgtcc ttaaaaaatc aagtccgatc ctttatcgag
     2881 aagatggaac tacgatggag ctcgatcaag agaaaaatac ggcctgataa atggaggaat
     2941 aaagctgact ccgacgacgc gaaccattcg ctcatcggtt gtcggctttt tccaccttat
     3001 aggaaatgaa ctaatggaca tacataactt atggaggtga cttccgcatg ccaaatcgaa
     3061 aaaagctgaa cgtgccttta ttgcctttgc gcgggatgtt ggtttacccg acgatggttc
     3121 tacatattga cgtcgggaga gagcgctccg tatttgcggt tgaacatgca atcgcgaatg
     3181 gcaattacat catgcttgct tcacaaaagg atacaaccgt tgaaaatccg gatgcagaag
     3241 atttatacca ta
//
LOCUS       DASULI010000452         4460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1840893,
            whole genome shotgun sequence.
ACCESSION   DASULI010000452 DASULI010000000
VERSION     DASULI010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840893"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /gene="gcvT"
                     /locus_tag="VFX34_09790"
     CDS             <1..631
                     /gene="gcvT"
                     /locus_tag="VFX34_09790"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5565244.1"
                     /translation="VLQKLTSEPLDEIKFFRFKEDVEISGHRVLVSRTGYTGEDGFEI
                     YGSPESIVALWPAILEAGEEEGVVPAGLGARDTLRFEAGLPLYGQELSKDITPLETGL
                     GFVVKLNKEADFIGKDVLAAQKENGVPRKLVGLEMIDKGIPRTGYKVFIGEEEIGEVT
                     TGTQSPTLKKNIGFALLATEHTAEGTEVEVEIRGKRLKAVLIATPFYKR"
     gene            657..2003
                     /gene="gcvPA"
                     /locus_tag="VFX34_09795"
     CDS             657..2003
                     /gene="gcvPA"
                     /locus_tag="VFX34_09795"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5565245.1"
                     /translation="MKHRYIPMTETDREEMLSAIGVATVDELFEDIPEKVRFKGELNI
                     KPAKSESALTKELAQLAAKNADARTYASFLGAGVYDHYKPIIVDHVISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTMICELTGMDIANSSMYDGGTSLAEAGTMAAGHTRRTKILV
                     SETVHPEARDVVASYAAGQYIDVVTIPHKDGVTDLALLEELLDENTAAVLVQYPNFFG
                     QVEDLQKIGDITHDKGGLLVVSANPLALGVLTPPGKLGADITVGDAQPFGISEQFGGP
                     HCGYFATTKKLMRKMPGRLVGETTDAEGRRGYVLTLQAREQHIRRDKATSNICSNQAL
                     NALAASVAMTALGKAGTKEIAYQNIAKTAYAKSAFEKAGFEVKYGGAHFNEIVVALKN
                     PVKEVNAKLLEKGIIGGYDLGLTYPELSNHVLIAVTEQRTKEEIDALVQEMEALHA"
     gene            1996..3474
                     /gene="gcvPB"
                     /locus_tag="VFX34_09800"
     CDS             1996..3474
                     /gene="gcvPB"
                     /locus_tag="VFX34_09800"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5565246.1"
                     /translation="MHKDNQPLIFEITKEGRIGYSLPELDVPEIDLSSHLPGGLLRNE
                     SPELPEVSELDIMRHYTALSNRNHGVDTGFYPLGSCTMKYNPKINEAVARFPGFANIH
                     PLQDESTVQGAMEMMYDLQEHLEEITGMDEVTLQPAAGAHGEWTALMMIRAFHEANGE
                     SHRTKVLVPDSAHGTNPASATVAGFDTVTVKSNEFGLVDIDDLKSKVGPDTAALMLTN
                     PNTLGLFEEDILQMAEIVHGVGGKLYYDGANLNAVMAKARPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVTKELAPFLPKPILVKEDDRYTFQYDVPQSIGRVKPFYGNFGIY
                     LRAYTYIRSMGPDGLKAVTENAVLNANYMMRRLEPYFDLPYTQHCKHEFVLSGRRQKK
                     LGVRTLDMAKRLLDFGFHPPTIYFPLNVEEAIMIEPTETESKETLDSFIDVMIQIAKE
                     AEENPEIVQEAPHTTVINRLDETKAARQPVLRYTKEEELVNA"
     gene            complement(3543..3920)
                     /locus_tag="VFX34_09805"
     CDS             complement(3543..3920)
                     /locus_tag="VFX34_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5565247.1"
                     /translation="MDYYILGAVLLAIILYFVIMMFRVKKAVTNLTQEQFIEGYRKAQ
                     LIDVREPKEFEAGHILGARNIPYSQFRQRFKEIRPDKPVYLYDQNGGKSSRAALYLKK
                     RDYTQLYQLQGGFRTWTGKVKSK"
     gene            4027..>4460
                     /locus_tag="VFX34_09810"
     CDS             4027..>4460
                     /locus_tag="VFX34_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018661562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octanoyltransferase"
                     /protein_id="HEX5565248.1"
                     /translation="MGKTVWGFINSGKCSPSYNMALDEALLEWHSRGEIGPVLRFYEW
                     EPATLSIGYFQSVEKEIDMDAVEKHGLGFVRRPTGGRGVLHEHELTYSVIVTEEYPGM
                     PETVTEAYRVISGGLLEGFRNLGLHAEFSLPASKEQSDNLKKP"
BASE COUNT         1249 a          970 c         1149 g         1092 t
ORIGIN      
        1 agtattgcag aaattgactt cggaaccatt ggatgaaatc aagttcttcc gtttcaaaga
       61 agacgtcgaa atttccgggc accgggttct cgtttcccga actggttata cgggtgaaga
      121 cggtttcgag atttacggat ccccagaatc gattgtcgct ctgtggccag caatcttaga
      181 agcgggagaa gaggaagggg ttgtgcctgc aggtttaggc gcccgagata ctctccgatt
      241 cgaagcgggc ctgccgcttt acggccagga attgtcgaaa gatattacac cgctggaaac
      301 aggccttggt tttgtcgtga aattgaataa ggaagcggat ttcataggca aagacgtgct
      361 tgcggcacaa aaagagaacg gtgttccaag gaagcttgta ggtctggaaa tgatcgataa
      421 aggaattcca cggaccggat ataaagtgtt catcggtgaa gaggaaatcg gggaagtcac
      481 tacgggaact caatcgccta cattgaaaaa gaatatcgga ttcgcacttc ttgcaactga
      541 acatacagct gaaggaactg aagtcgaagt cgaaatccgt ggaaaacgtc taaaagcggt
      601 attgattgcc acacctttct ataaacgata attgaatgaa gaggagacat gttgatatga
      661 agcatcgtta tattcctatg acggaaacgg atcgggaaga aatgctatca gcaatcggag
      721 ttgctacggt tgatgagctt tttgaagata ttccggaaaa agtccgtttt aaaggtgaat
      781 tgaatattaa accagcgaaa tcggagtccg ctttgacgaa ggagcttgct cagcttgcag
      841 cgaaaaacgc ggatgcgcgc acatatgcat ctttcttagg agcaggtgta tatgatcact
      901 ataaaccgat catcgttgat catgtcattt cgcgttccga gttctatact gcctatacac
      961 cgtatcaacc ggaaatttcc caaggggaat tgcaagccat ctttgaattc caaacgatga
     1021 tttgcgaatt gacgggcatg gacattgcca actcatctat gtatgacggc ggcacttctc
     1081 tagcggaagc tgggacgatg gcggcaggac atacacgccg cacaaagatc ctcgtttccg
     1141 aaacagtcca tccggaagcg cgggacgtag ttgcgtctta tgcggcaggt caatatatcg
     1201 atgtcgtgac gatccctcat aaagatgggg tgacggattt ggctttgctt gaggaattgc
     1261 tcgatgagaa tacagcagcg gttcttgtcc aatacccgaa tttcttcggc caggtggaag
     1321 accttcaaaa aatcggtgat atcactcatg acaaaggcgg gttgctagtc gtatcggcga
     1381 accctctagc tctaggtgta ttgacgcctc caggcaaatt gggtgctgat atcactgtgg
     1441 gagatgcaca accgttcggc atttccgaac agttcggcgg accgcattgc ggatactttg
     1501 caactacgaa aaagctgatg agaaaaatgc cgggaagatt ggtaggggaa acgactgacg
     1561 ctgaaggcag acgcggatac gtactgacgc ttcaagcacg tgagcagcat atccgacgcg
     1621 acaaagcgac atccaatatt tgttcgaacc aagcattgaa tgcccttgca gcttcggttg
     1681 cgatgacagc tttaggaaaa gccgggacga aggaaatcgc atatcaaaac atagccaaaa
     1741 cagcatatgc aaaaagcgcg tttgagaaag ccggatttga agtgaaatac ggcggagccc
     1801 attttaatga aattgttgtg gcacttaaga atcctgtaaa agaagtgaat gcgaagcttc
     1861 ttgaaaaagg aatcatcggc ggatacgatc ttggattgac gtaccctgaa ctatccaacc
     1921 atgtattgat tgctgtgact gaacagcgta cgaaagaaga aatcgatgca cttgtgcagg
     1981 aaatggaggc ccttcatgca taaagataac caaccgctca tttttgaaat tacaaaggaa
     2041 ggccgcatcg gctatagctt gcctgagctg gatgttcctg aaatcgatct ttcaagccat
     2101 ttgccgggag gtcttctcag aaacgaatcg ccggagcttc ctgaagtttc cgagctagat
     2161 atcatgcgcc actatacagc attatccaat cgtaaccatg gggttgacac aggcttctat
     2221 ccacttggat cttgtacgat gaaatataat ccgaaaatca atgaagcagt ggcaagattc
     2281 cctgggtttg caaacattca cccattgcag gacgaatcga ctgtccaagg tgcaatggaa
     2341 atgatgtatg atctgcaaga acaccttgaa gaaattacgg gaatggatga agtgacgctt
     2401 caaccggctg caggtgcaca cggagaatgg acggccctta tgatgatccg tgcattccac
     2461 gaagcgaacg gcgagtcaca tcgtacgaaa gtgcttgtgc cggattccgc gcacggtaca
     2521 aaccctgctt ccgctacagt cgcaggcttt gacacggtaa ccgtcaagtc gaatgaattc
     2581 ggacttgtcg atatcgatga tctgaaatcg aaggtcggac cagatactgc cgcccttatg
     2641 cttacgaacc cgaatacact gggcctcttt gaggaagaca ttcttcaaat ggctgaaatt
     2701 gtacacgggg taggcggaaa attgtattat gatggcgcaa acttgaatgc cgtcatggcg
     2761 aaggcccgcc ctggggacat gggcttcgat gcggtccact tgaacttgca taagacattt
     2821 acgggcccac acgggggcgg cggtccagga tcaggccctg tcggcgtaac gaaggaattg
     2881 gcaccattct tgccgaaacc gattcttgtc aaagaggatg accgttatac attccaatat
     2941 gacgttcctc aatcaatcgg gcgcgtcaaa cctttctacg gaaacttcgg catctatttg
     3001 cgtgcataca catatatccg ttcaatgggt ccggatggct tgaaagctgt cacggagaac
     3061 gcggtattga atgcgaacta tatgatgcgc agacttgagc cttatttcga tctgccttac
     3121 acgcagcact gcaagcatga attcgtacta tccggtcgtc gccagaagaa attgggcgtt
     3181 cggacactgg atatggcgaa gcgactgctt gacttcggat tccatccgcc tacgatctat
     3241 ttccctttga atgttgaaga ggcgatcatg atcgaaccga cggaaacgga atcgaaggag
     3301 acgcttgatt ccttcatcga tgtaatgatc cagattgcga aagaagcaga agagaatccg
     3361 gaaatcgttc aggaagcgcc tcatacgaca gtgatcaacc gtcttgatga gacgaaggcg
     3421 gctcgtcaac ctgtgttgag atatacaaag gaagaagagc ttgtgaatgc ttgatatgga
     3481 ataatgcaat aaagaaagga gaggcccagt aaccgggtct ctcctttatc tattacagct
     3541 gattatttac ttttcacttt gcctgtccac gtacggaatc cgccttgtag ctggtagagc
     3601 tgggtatagt cccgcttctt caaatataaa gcggctcgtg aactcttgcc gccgttctga
     3661 tcataaagat agaccggctt gtccggacgg atttctttga agcgctgacg gaactgtgaa
     3721 tatgggatgt tgcgggcgcc taaaatatgt ccagcctcga attccttcgg ttcacgcaca
     3781 tcgatcaatt gcgcttttcg gtatccttcg atgaattgct cctgtgttaa atttgtaact
     3841 gcttttttga cacgaaacat cataatgacg aagtacaaaa tgatggcgag tagaactgct
     3901 ccaagaatat aataatccac aaccactttt cccctttctt tctatagttc cattataaag
     3961 aatgagtagc gcttgttcaa tgggaatgat aaaatgatat gcattgcggg taagggggta
     4021 gatgacttgg ggaagacggt ttggggtttt atcaattcag gaaaatgttc tccatcatac
     4081 aatatggcgc tggatgaagc gcttttggaa tggcatagca gaggcgagat cggacctgtg
     4141 ctgcgttttt atgagtggga gccggcaacc ttgtcgatcg gttatttcca aagcgttgaa
     4201 aaggaaatcg atatggatgc tgtcgagaag cacggcctcg gattcgtacg tcgcccgaca
     4261 ggcggcagag gtgtcctgca tgagcacgaa cttacatata gcgtcatcgt tacagaagaa
     4321 tatcccggga tgccggaaac ggtcacagag gcttatcggg tcatttcagg aggtctttta
     4381 gaaggcttcc ggaacctcgg actgcatgcc gaattttcat tgcctgcttc caaggagcaa
     4441 agcgataact tgaaaaagcc
//
LOCUS       DASULI010000453         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_91989, whole
            genome shotgun sequence.
ACCESSION   DASULI010000453 DASULI010000000
VERSION     DASULI010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91989"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1557
                     /locus_tag="VFX34_09815"
     CDS             388..1557
                     /locus_tag="VFX34_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565249.1"
                     /translation="MLSNPVIATTLIFALIALGEWLSIVSRARVPMLLTAMVGFLLLI
                     WTGIFPKDILEKSQFPALGAILIGPAILHMGTMIPFSLLKSQFKAVIIALGGILVSGI
                     LILTIIPLIFDYPTAVAGVGPISGGIIALIITSEKLKELGLTSLVIIPALIVAFQGLM
                     GMPLAMNFMRRYAVKIQENIDNGTFIPMVSEADTLKEEEKSKKQGPFRSSTTLKLFFI
                     FVGAAVGVALGTVTPIHYTLWCLAIGVIGLKIGVFEPKTLEKANSFTITMIGIIFVVI
                     GSMAGVTPSQVLSNLPSILLILVIGTFGIALGGFLMSKLVKWHPYKGMPVALTALFGF
                     PGDYILCEEVSRSVARNEKEAKAIFDELLAPMLIGGFTTVTVASVVIAGILVQTL"
     gene            1574..2818
                     /locus_tag="VFX34_09820"
     CDS             1574..2818
                     /locus_tag="VFX34_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020006812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5565250.1"
                     /translation="MTKTIIKNGSLIDGNGGEPKHGQIVVIDGNRIDYVGEEDGYSAT
                     GDEQVIDAAGGSILPGFIDTHVHMMLEFSPIQERLATPFSFMYYQAAQYLKATLDAGV
                     TSVRDALGTDLGVKKAVDEGLIPGPRLQLSINALTITGGHGDGYTVSGNVLDLLPSPY
                     PGMPNGVCDGVEEVRKKTREMLRAGAEVIKVHATGGVLSATDHPEFTQFSLEELKVIV
                     EEGHFRKGVKVMAHAQGAEGIKNAVRAGVHSIEHGIFIDDEAIDLMIENGTYLVPTLL
                     APVAVLETAAETGMPETAVQKSKEVIQHHIDSFQKAYKAGVKIAMGTDAGVMKHGTNL
                     RELGLMADAGMSPMDAIVASTKTAAECLGWDDRVGTLETGKLADIIIVKGNPLEDIHS
                     LADNDNIQVVIKDGKVEKDLTE"
     gene            2922..>3541
                     /locus_tag="VFX34_09825"
     CDS             2922..>3541
                     /locus_tag="VFX34_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX5565251.1"
                     /translation="MNLDKYLKRFLASKDAQPSLQQLSELQRLHMTGIPFENLDVIRR
                     VPIYLNLNTIYEKIVLRNRGGYCYELNGLFHWLLTELGYDASLIAATVYRPNGQWAKP
                     ETHAAILVQLDEPYLVDVGFGDSTILPIPLNGNPQTDVSGTYSIQLSGNDVFDLIRTR
                     NGESRPLYRFSTSAKQLADFHEGCVFNQVSPESTFTHVDIATKATPT"
BASE COUNT          949 a          825 c          841 g          926 t
ORIGIN      
        1 tttcgtctgg tcattaaatc cgacaaggag gaataggcat gccccaatga tcgccatgca
       61 aatcaaatta tgttttctca tatgaaattc cccgcattct ctcatcggat tttctatcat
      121 tttatcgata tccgaattat ctttctatta tcctaacata aatctttagt cttgaatacc
      181 cttctattca taataagctt aaagtattac atttttttcg caaatcgttg tttcaatagt
      241 gtatgcgctt acaaatcgaa acaatagtag taatcgtcaa aatactattt acaaattaaa
      301 ttcacgctga tatattggat atcacttaaa actaagaaat catttcatag ttgaaagtat
      361 aattttttgc atctaggagg agccatcatg ttaagtaatc ctgtaatcgc cacaactttg
      421 attttcgcat taatcgcatt gggcgaatgg ctgtcaatcg tgtcaagggc cagagtcccg
      481 atgcttctca ccgcgatggt aggattcctt ctcctcattt ggacaggcat cttcccgaaa
      541 gatattttgg agaaatccca atttcctgct ctcggagcca tcttaatcgg cccggcaatt
      601 ttgcatatgg ggacaatgat ccctttttca ttgctcaaaa gccaattcaa agctgtcatt
      661 atcgccctcg gaggcatact cgtttccggg atcctcatat tgacgattat ccccctgatt
      721 tttgattacc cgacggctgt cgcaggagta ggtccgatca gcggcggcat catcgccctt
      781 atcatcacat cagaaaaact gaaagaactc ggtcttacga gcctagtcat cattccggcc
      841 ctgattgtcg ctttccaagg cctgatgggc atgccgcttg caatgaactt catgagaaga
      901 tacgcagtga aaattcagga aaacatcgat aatggaacct tcatcccaat ggtgtcggaa
      961 gcggatacat tgaaggaaga ggaaaaaagt aaaaaacaag gtccgttcag atcgagcaca
     1021 acgttgaagt tgttcttcat ttttgtcggc gcggctgttg gagtcgcact cggcaccgtc
     1081 acgccgatcc actataccct ttggtgcctc gcaatcggcg tcatcggtct gaaaatcgga
     1141 gtcttcgaac cgaaaacgct tgaaaaagca aactcattca cgattacgat gatcggcatc
     1201 atctttgtcg tcatcggatc catggccggt gttacacctt cacaagtgtt aagtaacttg
     1261 ccaagcattc tccttatcct cgtcattggc actttcggaa ttgccttagg cggattcctc
     1321 atgtcgaagc ttgtgaaatg gcatccgtat aaaggtatgc cagtcgcgtt gaccgcattg
     1381 ttcggctttc cgggcgatta tattctttgc gaagaagtga gccggagtgt cgcgagaaac
     1441 gagaaggaag cgaaagccat tttcgatgaa ctattggcac cgatgctcat cggcggattt
     1501 acgactgtga cagtggcttc ggtcgttatt gcaggaattt tagttcagac attataattc
     1561 ggagggattt acgttgacaa aaacaattat taaaaatgga tcacttatcg atgggaacgg
     1621 cggagaaccg aaacatggcc agattgttgt cattgacggg aatcggattg attatgtagg
     1681 tgaggaagat ggctactccg cgacgggtga cgaacaagtc atcgatgctg caggcggatc
     1741 gatccttccg ggattcatcg atacgcacgt ccatatgatg ttggagttct cgccaatcca
     1801 ggaacggttg gccactcctt tctccttcat gtattatcaa gcggcccaat atttgaaagc
     1861 gacattggat gcgggtgtca cttcagtgag agatgcgctc ggcaccgatc taggagtgaa
     1921 aaaagcagtc gacgaaggtc tcatccccgg acctcgcttg cagcttagca tcaacgcctt
     1981 gacaatcaca ggtggacatg gcgacggcta tacggtttcc ggtaatgtgc tcgatctgtt
     2041 gccttcccca tacccgggca tgccgaatgg ggtttgcgat ggcgtggaag aggtgcgcaa
     2101 aaagacgcgt gaaatgctgc gtgcaggtgc agaggtcatt aaagtgcatg caaccggcgg
     2161 tgttttaagc gcaaccgacc atccggaatt cactcaattc tcgttggagg agttgaaagt
     2221 catcgtcgag gaggggcatt tcaggaaagg cgtcaaggtc atggcccatg cacaaggagc
     2281 ggaaggcatt aaaaacgctg tccgcgcggg tgtgcattcc attgagcacg gcatcttcat
     2341 tgacgatgaa gcgattgatc tgatgattga aaacggaaca tatctagttc cgacgcttct
     2401 agctccggta gctgtattgg aaaccgcagc agaaacgggc atgccggaaa cggctgtcca
     2461 gaagtcgaag gaagtgatcc aacatcatat tgacagcttc cagaaagcct ataaagctgg
     2521 tgtcaaaatc gcgatgggga cagatgcggg agtcatgaag cacggaacga acctgcgcga
     2581 actgggattg atggcggatg caggcatgag cccgatggat gcgatcgtcg cttcgacgaa
     2641 aaccgcagcg gaatgtttag gctgggacga tcgtgtcgga acgcttgaaa ccgggaagct
     2701 tgccgacatt atcattgtaa aaggcaatcc gctcgaagat atccattcgc ttgcggataa
     2761 tgacaatatc caagttgtca tcaaagacgg aaaagtggag aaggatttaa ctgaataatg
     2821 cattggccac tgctgacaac cctcctcgca gtggccattt tttcaattaa tatatgttct
     2881 atcaatggta tactattcgt aatcgtaagg aggctgatag aatgaatctt gataaatacc
     2941 tcaaacgctt tctcgcatca aaggacgccc aaccttccct gcagcagttg tcggaactgc
     3001 agcgtctaca tatgactgga attccttttg aaaacctcga cgtcatccgg cgggttccga
     3061 tctatttaaa cttgaatacg atttacgaaa aaatcgtcct acggaatcgc ggaggctatt
     3121 gctatgaatt gaacggctta ttccattggc tgttaacaga gcttggatat gacgcttccc
     3181 ttatcgccgc caccgtatat cggccgaatg gacagtgggc gaagcccgaa acgcatgccg
     3241 ccatccttgt ccaattggat gaaccctatc ttgtggatgt cggattcggc gattccacaa
     3301 ttctgccgat tcccttgaac ggcaatccac aaacagacgt cagcggcact tattcgattc
     3361 agctatccgg caatgatgtt ttcgatttga ttcgaacccg aaacggggaa tcgcggccat
     3421 tgtatcgatt ttccacatcg gcaaagcaac tcgccgattt ccatgaaggc tgtgtattca
     3481 atcaagtctc acctgaatcg accttcacgc atgtcgacat cgccactaaa gcgacgccta
     3541 c
//
LOCUS       DASULI010000454         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_92171, whole
            genome shotgun sequence.
ACCESSION   DASULI010000454 DASULI010000000
VERSION     DASULI010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92171"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..819)
                     /locus_tag="VFX34_09830"
     CDS             complement(31..819)
                     /locus_tag="VFX34_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017379988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5565252.1"
                     /translation="MWKWEAEGQPKAVVVIVHNAYEHHRRYAWLIQKLRNGGFHVVTS
                     DLPGHGSESGRRTHDEPFESYVDHVKKMIDIALQDNLPLFVLGHGLGATIVMHILQRE
                     KLECAGFIFSSPWLSMQHQPSKFSGVLTKLSSSMKVNHDIPIEMLTRNYDMYVEFQND
                     RDYSSVVLASWFRELLAMMKSITSHEESIFDTPILMHTGEQDQITEVSFAKRWLLNQQ
                     LSEFQYKEWKRLYHDLYQEPEREEVYLYVESFMNNVLRSLGYIV"
     gene            902..1414
                     /locus_tag="VFX34_09835"
     CDS             902..1414
                     /locus_tag="VFX34_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016994472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5565253.1"
                     /translation="MIYSYNGTTPKIDPSVYVADYVTITGDVTIGASSSIWFNTVIRG
                     DVAPTIIGERVSIQDLSCLHQSPGKTLLIEDDVTVGHKALLHSCIVRKGALIGMGAII
                     LDGAEIGEGAFIGAGSLVPPGKKIPPGCLALGSPAKVVRELNDEDRAEMKRVLDSYVE
                     KGRIYKELTE"
     gene            complement(1750..2952)
                     /gene="metK"
                     /locus_tag="VFX34_09840"
     CDS             complement(1750..2952)
                     /gene="metK"
                     /locus_tag="VFX34_09840"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5565254.1"
                     /translation="MTNRRLFTSESVTEGHPDKMCDQISDAILDAILEEDPNARVACE
                     TTITTGLVLVIGEITTTTYVDIPKVVRDTVKEIGYTRAKFGFDWETSSVLTSIDEQSP
                     DIAASVDQALEAREGTMTDEELEAIGAGDQGLMFGYACNETPELMPMPISLSHKLSRR
                     LAQVRKDDTLDYLRPDGKTQVTVEYDEDNNPVRIDTIVISTQHHPDITLEQIQNDIKE
                     HVIYPVVPAELLDEETKYFINPSGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAF
                     SGKDATKVDRSASYAARYVAKNIVAAKLADRCEVQLAYAIGVAKPVSISIDTFGTGKV
                     EESKLVEIVRELFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVELPWEQTDKAEDL
                     IAKAGLTV"
     regulatory      complement(3134..3252)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          861 a          810 c          736 g          928 t
ORIGIN      
        1 caaaacaacc actcccctaa tggttttaag ttatacaata tagccgagcg agcgaagtac
       61 attgttcatg aatgattcaa catacaaata cacttcttcc cgctcaggct cttggtatag
      121 atcgtggtac agccttttcc attccttgta ttggaattca gatagctgct ggttcaagag
      181 ccatctcttt gcaaatgaaa cttcggttat ttgatcctgt tctcctgtat gcataagaat
      241 cggcgtgtca aagatgcttt cttcatgact agtgatggat ttcatcattg caaggagctc
      301 gcggaaccaa gatgcaagga caacggaact atagtcccgg tcattctgga actctacata
      361 catatcatag ttccgcgtca acatttcaat tggtatgtca tgattcactt tcatggaact
      421 tgatagtttc gtcaataccc ccgaaaactt tgaaggctga tgctgcatgg agagccaagg
      481 tgaactgaaa atgaagcctg cgcattccag tttttcacgt tgcagaatat gcatgacgat
      541 cgtggcgcct agaccgtgac caagcacgaa taaaggcaaa ttatcctgga gcgcaatgtc
      601 aatcatcttt ttcacatggt ccacatatga ctcgaacggc tcatcatggg tccttcttcc
      661 gctttcggaa ccgtgtcccg gcaaatcgct tgtgacgaca tggaagccgc cgttccgaag
      721 cttctggatg agccacgcat accgccgatg atgttcgtaa gcattatgga caatgacaac
      781 gaccgctttc ggctgtccct ccgcttccca tttccacatg gcgctcacct ctcctttatg
      841 tagttgaaag ttttgtgtta ggataaatta tacatgattt ttagaaaaga ggagacgttt
      901 catgatttat tcatacaacg gaacaacgcc gaaaattgat ccatccgtct atgttgcgga
      961 ttatgtgacg ataacaggcg atgtgacaat cggggctagc agttccatct ggttcaatac
     1021 ggtcatcaga ggagatgtcg ctccgacgat catcggcgaa cgggtcagca tccaagactt
     1081 aagctgtctt catcaaagcc caggaaagac tttattgatt gaagatgatg tgactgtcgg
     1141 ccataaagca cttttacata gttgcatcgt ccgaaaaggc gcgctgatcg gtatgggtgc
     1201 catcattttg gacggagcgg aaatcggcga aggcgcgttc atcggggcag gcagcctcgt
     1261 accgcccggc aagaaaatcc caccgggctg cctcgcgctc ggttcgcctg caaaagtcgt
     1321 ccgtgaattg aacgatgaag atcgggcgga gatgaagaga gttttagaca gctatgttga
     1381 aaagggccgg atctataagg aattgactga gtaaaaaggg tgctgtccaa aaacgggcag
     1441 cattttattt ttttgaaacg agtaaaacaa tgattcctgc cacgatagca caagcaacaa
     1501 ctccgataag tccgagcaac atgataacgc taattcccat cattgaatcg ccttccccac
     1561 ttttctagtt atatagtaaa cgatgtgaat ctgttaaggt tcccgatata agttgacgat
     1621 tgctaagttc tcgtttcttc ctgatgactt ctcatttttc tggaaagagt tatcgttctc
     1681 ctccaaaacc ttttcattcc acaataaaaa aggaagcgta gattttccac gcttcccaat
     1741 ttcatattat caaacagtca aaccagcttt tgcaattaga tcttccgctt tgtccgtctg
     1801 ctcccaagga agttcgacgt ctgtacgtcc gaaatggccg tatgccgcag tctgtttgta
     1861 gatcgggcgg cgaaggtcga gcattttaat aatgcctgcc gggcgtaagt cgaatagctc
     1921 gcgcacgatt tcgacaagtt tgctttcctc gactttgccc gttccgaatg tatcgatgga
     1981 aatcgaaaca ggttttgcaa caccgattgc gtaagccaat tgaacttcac aacgatccgc
     2041 cagtttagcc gctacgatgt tcttcgctac atagcgcgca gcataggaag ctgaacggtc
     2101 cacttttgtc gcatcttttc cggaaaatgc accgccgcca tgacgtgcat agccgccata
     2161 tgtgtcaaca atgattttcc gacctgtcaa gcccgcatcc ccttgtggtc cgccgataac
     2221 aaagcggcct gaagggttga tgaagtattt cgtttcttca tctaataatt ccgccggcac
     2281 aaccgggtag atgacatgtt ctttaatatc attttgaatt tgttccaaag tgatatcagg
     2341 atgatgttga gttgagataa cgatggtgtc aatccgcacc ggattattat cctcatcgta
     2401 ttccacggtc acttgcgttt ttccatccgg gcgcaagtaa tcgagcgtat cgtctttcct
     2461 cacttgtgca agcctgcggg aaagcttatg ggataaactg atcggcatag gcatcaattc
     2521 aggggtttca ttgcaagcat aaccaaacat caagccttgg tcccctgctc cgatcgcttc
     2581 aagctcttca tccgtcattg tcccttcgcg tgcttccagc gcctgatcga cacttgccgc
     2641 gatgtcaggg gactgttcat cgatcgacgt caatacagaa gacgtttccc agtcgaatcc
     2701 gaatttcgcg cgtgtgtagc cgatctcctt cactgtatcc cgcacaactt tcgggatgtc
     2761 tacgtacgtt gttgtcgtga tttctccgat gacaagaacg agtcctgtcg taattgtcgt
     2821 ttcacacgct acgcgtgcat tcgggtcctc ttctaaaata gcgtccaaaa tggcatcaga
     2881 aatctgatcg cacattttgt ccggatgtcc ttctgttacc gattccgaag tgaataatcg
     2941 gcgatttgtc attgtagttt cctccttaac ggcttgcaaa tattgatacg gtactctaat
     3001 tgtcccagta agttcagcgc ctagagagtc gacgtcaaca tacacaagga ttaagggcct
     3061 gacaatataa aaagcccttc gctcacgtaa atatacatga ggaaaggcat aatttcaaaa
     3121 gcagcacctt tcactcttat cgtccaagga attcaacctt gctccaggtt tggcaccttc
     3181 tcgcggccta aatggtccac gcaggttgcc gggcttcata gggcctgtcc ctccgccagc
     3241 tcgggataag agtatccgtt caataatcaa tgctacgaaa aagtttgcac tttgtcaaac
     3301 atttttagac atatgtactt gagtttttca ttagt
//
LOCUS       DASULI010000455         4850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1084877,
            whole genome shotgun sequence.
ACCESSION   DASULI010000455 DASULI010000000
VERSION     DASULI010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084877"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..2370
                     /locus_tag="VFX34_09845"
     CDS             241..2370
                     /locus_tag="VFX34_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5565255.1"
                     /translation="MRTLARFVTKYYKAIMIAWIAIFIAMAIFATRLPGLLAGDGFRM
                     DAEHEQVMKIASEKFDLPAETMFVVFDKTSDETIASTLADIEKLNLASEIVSPLENEE
                     QYTKDVSYALLHFEKNGKDMSKVVTDIRKAIGDRKGIVLTGQSAITKDINSASQRDLM
                     KAEAIGLPIAIIVLLFAFGSLMASMVPLIVGVATVISTFGVLALVGGQMDLAIFVLNI
                     VPMLGLALSIDFSLLFISRYREERLKTDVLEAVATTIRTAGRSVIFSAFCVFIGLGAM
                     FIIKVEIFQNIALGGMIVVGMAVLSSVTLLPSILILLGDRLNTGRILKVKENGSDRWR
                     QFANAVIRRPVLITVAALVLLGIALIPVKNMDLTIPQIDSLPASYDTRASYELMDDTF
                     GLGKQSNVYIVAEREGGWSDADGLDAMKALEEDLIDDSLVDKLTTIFTASGVGSPEEW
                     EQAMQVPEMKAQLEPLLESFVKDDQLLIPVTLKAKGTSEEAQQWARDWSEQTEWDLLV
                     GGQAKFNQEIFDEIFGNIVSVLTIILVSTFIILMIAFRSVLIPLKAILMNIVGLSATF
                     GILVYIFQYGHFGLHPGTIALIIPILVFSLVFGLSMDYEVFLISRMQEEYAKTFDNDH
                     STVEGLATTSKIITSAALIMIVLTGAFAFTDVMPVKQIGVGIAIAVAIDATIIRLLLV
                     PSLMKLFGKWNWWMPFRKGPYKAGNWH"
     gene            complement(2652..2786)
                     /locus_tag="VFX34_09850"
     CDS             complement(2652..2786)
                     /locus_tag="VFX34_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565256.1"
                     /translation="MKGKTLVIGLVVFVLAVVAVIYFAMTAQFDKDRQEDGVTTTIVD
                     "
     gene            complement(2793..3257)
                     /locus_tag="VFX34_09855"
     CDS             complement(2793..3257)
                     /locus_tag="VFX34_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565257.1"
                     /translation="MLTFEQKQQIIEETFPELARKEVSMKRLNYHYEDSLYDKTVVVQ
                     HLHPNGNGFVYVEGIPGYEADDRGLVNIREATEEELIATIRDAIRALSTEEEEEEPIE
                     EVWENEQGQKLTLLEEEGFFNIYHGYNLEEGFGDYKEAVDYLKEENFKLSRG"
     gene            complement(3302..3952)
                     /locus_tag="VFX34_09860"
     CDS             complement(3302..3952)
                     /locus_tag="VFX34_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX5565258.1"
                     /translation="MTEKKDKRMKPKLSKEHELLRPSDIWNRDRFIEQARFEGGEMPS
                     ADGERLVFDNCVFKDMSFANSDWRGVEFVDVLFHTCDFSNVQMENAMLHRCEIINSKL
                     TGADMANTNLGHLLMKESDIRYANFNFSEMKEVEYAGSNLEDSDFYECQFNQVRFEKC
                     KLDNANFSETDLDGVDLSDSTYDRIEVTVPKIAGCIVSTEQALGFARALGLTVKDE"
     gene            complement(4208..>4850)
                     /locus_tag="VFX34_09865"
     CDS             complement(4208..>4850)
                     /locus_tag="VFX34_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5565259.1"
                     /translation="GPVLENIIARLKGSLEGVECPVVLDAGCGEGTHLQEVHSQLRTG
                     SVGIGIDLAKEGIAAASKAYPGIIWSVADLAAMPFADTSVDAILNVLSPANYAEFNRL
                     LKPGGMVVKVVPESGYLQELREAFYDGKQQKEEADPVERFREQFDAVETERVTYTFTL
                     SEDLLAPLIRMTPLTWNASEERVSEVLAAGMKEITIDLRVISGVRQSGKMAEW"
BASE COUNT         1155 a         1267 c         1100 g         1328 t
ORIGIN      
        1 cattcaaaac cacttccttc ggggagtggt ttttttcatt attgcagcaa agttgacgtc
       61 ggtttaagaa ctcattgaag ggaaatgaga actcttgatg ccaaaacggg aacttcttga
      121 tccccgccct cacttggata caattctcct aaattactcc ttcagaaaag acattcccat
      181 ccaatttcgc tatgataagg aatagacaaa agatggtttt catttagggg ggacgatggt
      241 atgcgcacac ttgcgcgttt tgttacgaag tattataaag caatcatgat tgcctggatt
      301 gccattttca ttgcgatggc catttttgcg acccgcttgc ctgggttgct tgcgggtgac
      361 ggcttcagga tggatgcgga gcatgaacaa gtgatgaaga tcgcttccga aaaatttgat
      421 ttgcctgcgg aaacgatgtt tgtcgttttc gataaaacaa gcgacgaaac gattgcatcc
      481 actttggcgg atatcgagaa gctgaatctt gcgtccgaaa ttgtttcccc acttgagaat
      541 gaagagcaat atacgaaaga tgtttcttat gcattgcttc attttgaaaa gaacggcaag
      601 gacatgtcta aagtcgtcac ggatatccgc aaagcgatcg gcgataggaa aggcattgtc
      661 ttaaccggcc aatctgccat tacgaaagac atcaacagtg cgagccagcg tgacctgatg
      721 aaggcggagg cgatcggctt gccgatcgca atcatcgttc ttctcttcgc attcggttcg
      781 ctcatggcct cgatggttcc gctgatcgtc ggcgtcgcaa ctgtcatctc gacattcggt
      841 gtgttggcgc ttgttggcgg acagatggat ttggctattt tcgtattgaa tatcgtcccg
      901 atgcttggtc tcgctctgag catcgacttt tcgcttcttt tcattagccg ctaccgggaa
      961 gagcgattga agaccgatgt gcttgaagct gttgcgacaa cgatccggac ggctggccgc
     1021 tccgtcattt tctctgcatt ttgcgtgttc atcgggcttg gcgccatgtt cattatcaaa
     1081 gtcgagattt tccaaaacat cgcgctcggc ggcatgattg tcgtcggtat ggcggttctc
     1141 agctcggtta cattgttgcc gtctattctg atcctgcttg gcgaccgttt gaatacaggc
     1201 cgcattctga aagtgaagga aaacggctca gacagatggc gacagttcgc caatgcggtg
     1261 atcagacggc ccgtccttat tacagttgcc gcactcgtct tattagggat cgctttgatt
     1321 cccgtgaaaa atatggactt gacgattcct cagattgatt cactgcccgc gtcatatgat
     1381 acacgtgctt cctatgaatt gatggatgac acattcggac tcggaaagca gtcgaatgtg
     1441 tatattgtcg cggagcgaga aggcggctgg tccgatgcgg acggattgga tgcaatgaaa
     1501 gcgttggaag aagacttgat agatgattct ttggtcgaca aactgactac aattttcact
     1561 gcaagcggag tcggttctcc cgaagaatgg gagcaagcga tgcaagtacc cgaaatgaag
     1621 gcgcagcttg agccgctcct tgagtcattc gtgaaagatg atcaattatt gatccccgtg
     1681 acgctgaagg ctaaaggtac gtccgaagaa gcgcagcaat gggcgcggga ttggtctgaa
     1741 caaacggaat gggatctgct ggtgggcggt caagcgaagt tcaatcagga aattttcgat
     1801 gaaatcttcg gcaatatcgt ttccgtgctg acgatcattc tcgtgtctac gttcatcatc
     1861 ctcatgattg cgttccgctc ggtgctcatt ccgttgaagg cgattttgat gaacatcgtt
     1921 ggattgtctg cgacgttcgg aatcctcgtc tacattttcc aatacggtca tttcgggctg
     1981 catccgggaa caatcgcgct catcattcca atactcgtct tcagccttgt cttcggcttg
     2041 agcatggact atgaagtgtt cctcatttcg cgcatgcagg aggaatatgc gaaaacattc
     2101 gataatgacc attcgacagt cgaaggcctt gcgacgacga gcaagatcat cacatccgcc
     2161 gcgttgatca tgattgtttt gactggcgca ttcgcattca cggatgtcat gccggtgaaa
     2221 cagatcggcg tcggcatcgc gattgcagtc gccatcgacg cgacaatcat ccgcttattg
     2281 cttgtcccga gtctgatgaa gctgttcggg aaatggaatt ggtggatgcc tttcaggaaa
     2341 ggtccttata aagctgggaa ttggcattaa acaaatgcgg aaggcggcga taagccaatc
     2401 gaacagcaaa gggttcgatt tgccgtattt ctgcgttctt tgcagaaata aaggcatcgt
     2461 gcatagtgct cgtaacgctt cgcttttact cgcaaacgcc gttctgcgtg acggcgttcg
     2521 ctggagaact tgaaaaaaag gcgctttttg ccttttcttt caagatcgaa gcgacccgag
     2581 aggctgccgt ctgaagctgg atgataacat aaaaaaactg tctggacgct tgtccggaca
     2641 gttttttcat gttaatcaac aattgtagtt gtgaccccgt cttcctgccg atctttatca
     2701 aactgtgctg tcatcgcaaa atagatgaca gcgacgactg ccaggacgaa gacgacgagc
     2761 ccgatgacaa gcgttttacc cttcatttgg cgtcaccccc tgctaagttt gaagttctct
     2821 tccttcaaat aatcaacagc ttccttgtaa tcaccgaagc cttcctccaa gttgtagcca
     2881 tgataaatat tgaaaaagcc ttcttcctca agcaacgtca gcttctggcc ttgttcattc
     2941 tcccatactt cctcaatcgg ctcttcttcc tcttcctcag tggatagcgc acggatggca
     3001 tcccggatcg tcgcaatcaa ttcctcttcc gtcgcttcac ggatgttgac gagtccgcga
     3061 tcatccgcct catagcccgg gattccttcc acatatacaa agccattgcc gttcggatga
     3121 agatgctgaa cgacgaccgt tttgtcataa aggctatctt cataatggta attgagtcgt
     3181 ttcatcgaca cttctttccg cgccagttcc gggaaagtct cctcaatgat ttgctgtttt
     3241 tgttcgaatg taagcatgct ttggccactc tttctttgtc aaatgtcttt acagtatacc
     3301 atcattcatc cttcaccgtc agaccgagtg cacgtgcaaa accaagcgcc tgctccgtcg
     3361 acacgatgca tccggcaatc ttcggcaccg tcacttcaat tcgatcgtat gtactgtcgg
     3421 ataaatccac gccatccagg tcggtttccg agaaattcgc attgtcgagc ttgcactttt
     3481 caaaccggac ctgattgaat tggcattcat aaaaatcgct gtcctccaag ttgcttcccg
     3541 catattccac ttctttcatt tccgaaaaat tgaagttggc gtacctgata tcgctttcct
     3601 tcatcaatag atggcccaag ttggtattgg ccatgtccgc tcccgtcagc ttcgaattta
     3661 tgatttcgca ccggtggagc atggcgtttt ccatttggac attcgagaag tcgcatgtgt
     3721 ggaataaaac atcgacgaat tccacgcctc tccaatcact attcgcaaac gacatatcct
     3781 tgaacacgca attatcgaaa acgaggcgct ctccgtccgc gcttggcatt tctccacctt
     3841 caaaccttgc ttgttcaatg aacctatcgc gattccatat atcggatggc ctcaacagct
     3901 catgctcctt cgacaatttg ggtttcatac gcttgtcctt tttctccgtc atgcaatccc
     3961 tcctatcttg tcttccattc gatgaaaatg acgagcagta tgacaactac gaacgccaac
     4021 atataaaacc aacttggaac tgcgctaatc ccgctgaaca tcctaccaac tccctttcct
     4081 tgcaacctac agatcccggt tatcaccggt tatctatcgt ttatcaccgc ttatagacag
     4141 attatcaccg cttagtctct gtttatcacc gctcacgccc actttgtcac cacttggaac
     4201 aggtttatca ccactcagcc attttccctg attgccgaac tcccgaaatc acccgcaaat
     4261 caatcgtgat ctctttcatc cctgccgcaa ggacttcact cacccgctcc tcacttgcat
     4321 tccatgtaag cggggtcatg cggatcaatg gcgcaagcag atcttccgac aaggtaaaag
     4381 tatatgtcac ccgctccgtc tcgaccgcgt cgaactgttc gcggaagcgc tccaccgggt
     4441 cggcttcctc cttctgttgt ttcccgtcat agaaagcttc ccgcaactcc tgcaaatacc
     4501 cgctttccgg taccactttc acaaccatgc cgcccggctt cagcaaccgg ttgaattctg
     4561 cgtaattcgc cggcgacaag acattcaata tcgcatctac actggtatcc gcaaacggca
     4621 tggcagcgag atcagccaca ctccaaatga ttccggggta agcctttgaa gctgccgcaa
     4681 tgccctcttt cgccaaatca atgccgatgc cgacacttcc agttcgcaac tgtgagtgga
     4741 cctcctgcaa gtgggtccct tccccgcagc ccgcatccaa tacgacaggg cattcaactc
     4801 cttctaaaga ccctttcaaa cgcgcaatga tattctcaag gacaggcccg
//
LOCUS       DASULI010000456         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_768280, whole
            genome shotgun sequence.
ACCESSION   DASULI010000456 DASULI010000000
VERSION     DASULI010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768280"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1370
                     /locus_tag="VFX34_09870"
     CDS             105..1370
                     /locus_tag="VFX34_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX5565260.1"
                     /translation="MSYEAKMMEMKKLLKKKAPQAEEIPPAPKHETAPPPAYEKRWLA
                     AGLTKEENEFGIVYKRTVVYEPHHQHGKYTLSGLLDTVERWRESKEIHPLAPNFSRPL
                     VFFDTETTGLKGTGTLIFLMGFIEYTEKAFTLTQYVLPGPDHEAAFLYATDLWKTPMS
                     IVMYNGKSFDMPQVQTRWTMNRNVLPQLLKHTEIDLLHGSKRIWKNEMDTFKLTAVEE
                     EQLGFFREDDIPGHMAPIIYQDAVKNGRAELLMKVLLHNEWDILSLVTLYIRSTDLLL
                     HDKAMSSVNAKTNIGKWYTDLKSFSRSKDVLMDVIAEYGPEEPKAHFHIGFLLKKEEL
                     HAEAIESFTIAAGGLEGRERIIAYEELAKLFEHKMKDPAKALHYTKEAILYLRQEPDM
                     TGRFKVRMGNEFTHREIRLKKKLFPGEAH"
     gene            1469..1765
                     /gene="gpsB"
                     /locus_tag="VFX34_09875"
     CDS             1469..1765
                     /gene="gpsB"
                     /locus_tag="VFX34_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003182873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEX5565261.1"
                     /translation="MEIKLDSKTILEKEFKTGLRGYHQEDVDLFLDQVIQDYEAFKKT
                     ISELRMENEKLRDELASATKRPAATNANTTNFDLLKRISNLEKHVFGSKLYDHE"
     gene            1838..2222
                     /gene="rnpB"
                     /locus_tag="VFX34_09880"
     ncRNA           1838..2222
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFX34_09880"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            2364..>3334
                     /locus_tag="VFX34_09885"
     CDS             2364..>3334
                     /locus_tag="VFX34_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX5565262.1"
                     /translation="MTNYKLVATSAMGLESIVADEVKELGFQTKTENGKVYFEGDETA
                     IAKANMWLRVADRVRIIVGEFTATTFDDLFEQTKSLPWEQYLPVDAKFPVAGKSVKST
                     LYSTPDCQAIVKKAIVERLKNAYNRVGFLDETGPLFKLEVSILKDKVTLTIDSSGAGL
                     HKRGYRVGQGDAPLKETMAAALVKLTRWNPDRPFVDPFCGSGTIAIEAAMIGQKIAPG
                     YNRDFSSEEWPWMDQAIWDRIREEAEDIANYDQPLDITGFDVDPKMIKIAQQNAVEAG
                     FMDLIRFEQRDIKDLTVEGLNGVLVGNPPYGERLGEIEEAEELARVIG"
BASE COUNT         1024 a          689 c          830 g          791 t
ORIGIN      
        1 ttaatctata gttcgtcaaa agtttcaaat caattctaca tcccagtcgt ttatttctat
       61 gatacgatag ccataaaata aagaatagaa gggggggaag attgatgtca tatgaagcta
      121 aaatgatgga aatgaagaag cttttaaaaa agaaagctcc ccaagccgag gaaatacccc
      181 cagctccgaa acatgaaaca gccccaccac ctgcttacga gaaaagatgg cttgcggctg
      241 gattgacgaa agaggagaat gaatttggta tcgtgtataa acggactgtt gtctatgaac
      301 ctcatcatca gcatgggaaa tatacgcttt ccggattatt ggatacagtg gaaagatggc
      361 gtgaatcgaa ggagattcat cctcttgcgc cgaatttttc gcgaccgctc gtgtttttcg
      421 atacggagac gaccggcttg aaaggcacag gtacactcat cttcctcatg ggcttcatcg
      481 aatatacgga aaaggcgttc acattgacgc aatatgtctt gcctggtccg gatcatgaag
      541 cggcattcct ttatgctacc gacctatgga agacaccgat gtcgattgtc atgtacaacg
      601 gaaaaagctt cgatatgcca caagtgcaga cgagatggac gatgaatcga aatgtgctgc
      661 cacaattgct gaagcatacc gaaatcgatc tgcttcatgg atcaaaaaga atctggaaaa
      721 atgaaatgga tacattcaag ttgactgcag tcgaggagga gcagcttggt ttcttccgtg
      781 aagacgatat tcctggccat atggcgccga tcatctatca agatgcagta aaaaacggtc
      841 gtgcagaatt gctgatgaaa gtcctgcttc ataacgaatg ggatattctt tccctcgtca
      901 ctctgtatat ccgctcaacc gacttgctct tacatgataa agcaatgtcc tctgtaaacg
      961 ctaaaacgaa tatcgggaaa tggtatacgg acttgaaatc gtttagccga agcaaagatg
     1021 tcctcatgga tgtcatcgcg gaatacgggc cggaggagcc taaggcacat tttcatatcg
     1081 gtttccttct gaaaaaggaa gagctgcatg cagaagcgat cgagtccttc accatcgctg
     1141 ccggtggttt agagggaagg gaaagaatta tcgcctatga ggaattggcg aagcttttcg
     1201 agcataaaat gaaggatccg gcaaaagcac ttcattacac aaaggaagcc attctttact
     1261 tgcggcaaga gccggatatg acaggtcgtt tcaaagtccg tatgggaaat gagttcaccc
     1321 atagggaaat aagattgaag aagaagctat ttcccgggga agcgcactaa ccgacaaaat
     1381 cctgcttttt agccgaaata gaaagacaaa gttgtacaaa atatgctata cttgcgtcat
     1441 gtaaacatag atggaagggc gtcatttcat ggaaataaaa cttgattcta agacgattct
     1501 tgaaaaagaa ttcaaaacag gtttacgcgg atatcatcaa gaagatgtag atctcttcct
     1561 ggatcaggtt attcaggatt acgaagcttt caaaaaaaca atctcagaat tgcgtatgga
     1621 aaatgagaag ctgagggatg aacttgcttc agcaacgaaa cgtccagctg caacgaatgc
     1681 aaatacaaca aacttcgatc tgttaaaacg gatttccaat ttggaaaagc acgtattcgg
     1741 cagcaagttg tacgaccatg aataagcagg ctgatggcgg agaagaaatt cgttgatttt
     1801 tgtcgaaaat tgcagtatac tattgtaacc atatcgtgaa ttcaggtaat cgctgcaacg
     1861 tacgttgtag aggaaagtcc atgctcgcac ggttctgaga tgaccgtagt gttcgtgctc
     1921 ggcgaaaaaa taagccgggg ctttgcgcaa tgcgcattga cggcggggat aagtcctaag
     1981 tgtgctttgc acatatgggc gaggtcccct gaaaagtgcc acagtgacgt agctggtccg
     2041 gaaacggctc agatggaacg cggtaaaccc cacgagcgag aaacccaaat tatggtaggg
     2101 gcactcttct gaaggaattg aacggaggaa gggacagaca ttttctgtag atagatgatt
     2161 accaccctaa gtacgaggcg caagccgttc gagtacacgg gaacaaaaca tggcttatcg
     2221 aatttgcgat ggttcaaata acaatcaatg ctctcctcat tcgtttggag agcatttctt
     2281 cgttatttgg aatatgaatt aatataaatg ccaatagtag gcagactaca tatagagacg
     2341 actagaattg gagttggaaa tgcatgacaa attataaatt ggtcgccaca tccgccatgg
     2401 ggcttgagtc gatcgttgcg gatgaagtga aagaacttgg cttccaaacg aaaaccgaaa
     2461 acggcaaagt ctattttgaa ggcgacgaaa ctgcaattgc aaaagcgaac atgtggcttc
     2521 gtgtcgcgga tcgtgtccgc ataatcgtcg gcgaattcac agctacgact ttcgatgatc
     2581 ttttcgaaca gaccaaatcc cttccgtggg aacaatactt gccagtcgac gccaaattcc
     2641 cggtagcagg taaatcagta aaatcgacat tgtacagcac acctgactgc caggcaatcg
     2701 tcaaaaaggc aatcgttgaa cgcctgaaaa acgcctataa ccgggtggga ttcctagacg
     2761 aaacgggccc tctttttaag cttgaagtat ccatcctgaa agataaagta acattgacaa
     2821 tcgattccag tggggccggc ttgcataagc gcggataccg cgtggggcaa ggggatgcgc
     2881 cactcaaaga gacgatggcg gcagctctcg taaaattgac gcgttggaat cccgacaggc
     2941 cgttcgtcga tccgttttgt ggatccggaa cgattgccat cgaagcggca atgatcggtc
     3001 agaaaatcgc accaggctat aaccgtgatt tcagcagtga agaatggcct tggatggacc
     3061 aagcgatttg ggaccgcatc agagaagagg cggaagacat tgcgaactat gaccagccgc
     3121 ttgatattac aggttttgac gtcgatccga agatgatcaa gatcgcacag caaaacgcag
     3181 tggaagcagg cttcatggat ctgatccgtt tcgagcagcg cgacatcaaa gatttgactg
     3241 ttgaaggttt gaacggagta cttgtcggca atcctcctta tggggaacga ttgggtgaaa
     3301 ttgaggaagc cgaggaactt gctcgcgtca ttgg
//
LOCUS       DASULI010000457         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1552151,
            whole genome shotgun sequence.
ACCESSION   DASULI010000457 DASULI010000000
VERSION     DASULI010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552151"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFX34_09890"
     CDS             complement(<1..710)
                     /locus_tag="VFX34_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5565263.1"
                     /translation="MKETKLWINGSWQAADRTYELRSPYSEELLATVARASVEDVKKA
                     VDGAGKAFQTFKEWTAYDRAELLYSVVRIMRERKEELANILTQEAGKPIRAARTEIDR
                     TIATYQFSAEEAKRIHGETIPLDAAPGVTDRIGWTKREPLGVVSAITPFNFPFNLVAH
                     KLGPAFAVGNTVVLKPANQTPLSALVMAEIFEEAGLPEGALQIVTGSGSELSDALISH
                     PSVRKVTFTGSVPVGLSI"
     gene            853..1695
                     /locus_tag="VFX34_09895"
     CDS             853..1695
                     /locus_tag="VFX34_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HEX5565264.1"
                     /translation="MSEFSLIMNTTYFQSKETIKEQVLELGIQPGDQLIVHSSLKAMG
                     WIAGGPQAVVEALMETVTSEGTIVMPAQSADNSEPSYWMLPPVPEAWHQPLRESIPAY
                     DPHLTPLRQMGKIAECFHRHPQTIRSPHPAHSFMAWGKNAADWMKEHPLEDSFGMTSP
                     LGKMVNADVKILLIGVGYDSCTALHLSEYLAPGLTHSLQGAAILHEGKRKWATYDMAD
                     VDSDLFPDIGEAFEKSHPHIVRTGALGQAACKVIPMKPLIEFGTDWVEQYRRTTIGTK
                     DTSL"
     gene            1804..>2063
                     /locus_tag="VFX34_09900"
     CDS             1804..>2063
                     /locus_tag="VFX34_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013308183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX5565265.1"
                     /translation="MRINKYLSETGIVSRRGADKWIAEGKVTINGELATLGSKVEEGD
                     DVHVDGKPVKKEEELVYLALNKPVGITSTTERHIEGNIVDFV"
BASE COUNT          537 a          496 c          487 g          543 t
ORIGIN      
        1 ttgattgata agccaacggg aacacttcct gtaaacgtca cttttcgaac agatggatgc
       61 gaaatcaatg catcgcttag ttcgctcccg cttcccgtta caatttgaag cgctccttcc
      121 ggaagacctg cttcttcaaa aatctccgcc attacaagtg cacttagtgg tgtttgattc
      181 gcgggcttca ggacaaccgt atttccgact gcgaatgcag gaccaagttt atgtgcaaca
      241 agattgaacg gaaagttaaa tggggtgatt gctgacacaa cgccaagcgg ttcccgtttc
      301 gtccatccaa tccggtccgt cacaccaggc gcagcgtcca atggaatcgt ctcgccatga
      361 atacgtttcg cctcttccgc tgaaaattga tacgtcgcaa tcgtccgatc gatttctgtc
      421 cgggctgctc gaattggctt gcccgcttcc tgtgtcaaaa tgttcgccag ctcttctttt
      481 ctttcccgca taatccgtac gaccgaatac agaagctccg cccggtcata tgcagtccat
      541 tctttaaagg tttgaaacgc cttccctgcc ccgtcaactg ccttcttaac atcttcaacg
      601 gaagctctcg caacagttgc cagaagctcc tcggaatatg gagaacgcaa ttcatatgtt
      661 cgatccgctg cttgccagct tccattgatc catagttttg tttccttcac gggaacacct
      721 cctgtacacc ttgtttaccc taaacgatag catgtttcga tcgttaattt tgtaaatttg
      781 ctttgcgtta ttggcgaatc acaccctccc cgttatactt taggaaacgt tggttttact
      841 ggggggtttc gcatgtcaga gtttagtttg attatgaata cgacgtattt tcaatcaaaa
      901 gagactataa aagaacaagt attggaatta ggaattcagc ctggcgatca gcttatcgtt
      961 cattcctccc tgaaagcaat ggggtggatt gcgggtggtc cccaagcagt tgtcgaagcg
     1021 ctcatggaga cggttacctc agaaggcaca attgttatgc cggcacagtc tgcggataac
     1081 tccgagccat cgtattggat gctgcctccc gttccggaag cttggcacca accccttcgg
     1141 gaatcaattc ctgcatacga tcctcatctg acgccactcc gtcagatggg caagatcgcc
     1201 gagtgctttc atcgtcatcc tcaaacgatt cgaagcccgc acccggcaca ttcctttatg
     1261 gcctggggaa agaatgccgc tgactggatg aaagagcatc cgcttgaaga ttcatttgga
     1321 atgacatccc ccctaggcaa aatggtgaat gcagatgtga agatcttgct cattggcgtg
     1381 gggtacgatt cttgcacagc gctccattta tccgaatacc tcgctcccgg cctgacgcat
     1441 tccctacaag gtgcggcaat tctccatgaa ggaaagcgta aatgggcgac gtatgatatg
     1501 gcggatgtgg attcagatct ctttcctgac atcggggaag catttgaaaa aagtcatcct
     1561 cacatcgtgc gtacgggagc acttggacag gcagcctgta aagtaattcc gatgaaaccg
     1621 cttattgagt tcggaacaga ttgggtagaa caatatagac gaacgaccat cggaacgaaa
     1681 gatacttcct tataagtaca tcaaaatagt aacaatcaag ttttgtagca ttggagcaac
     1741 tttctctcat ttttacttga ttggcgttat acttgcaata cgataaagag caggtgaaaa
     1801 gctatgcgaa ttaataaata tttgagcgaa acggggattg tttcccggcg tggtgcagac
     1861 aagtggattg cagaaggaaa ggtcacaatt aatggcgagc tggcgaccct tggcagcaaa
     1921 gttgaagagg gcgacgacgt tcatgtggat ggaaaaccgg tgaaaaagga agaggagctc
     1981 gtctatctag cactcaataa gccggttggt attacgagta caacggaaag gcatattgaa
     2041 gggaacattg tggactttgt caa
//
LOCUS       DASULI010000458         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_539221, whole
            genome shotgun sequence.
ACCESSION   DASULI010000458 DASULI010000000
VERSION     DASULI010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539221"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1482)
                     /gene="fumC"
                     /locus_tag="VFX34_09905"
     CDS             complement(100..1482)
                     /gene="fumC"
                     /locus_tag="VFX34_09905"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5565266.1"
                     /translation="MEYRIERDTIGEIKVPADKLWGAQTQRSKQNFKIGGERMPIGVI
                     RAFAHLKKSAAIASHSFGNLSEAKMKAISAAADEISSGKLDDHFPLVVWQTGSGTQSN
                     MNVNEVIAHRGNELLAEWGEEERLHPNDDVNKSQSSNDTFPTALHVAGVIAVETELLP
                     ALNKLKETLAQKSEEFMDIIKIGRTHLQDATPLTLGQEISGWHRMLEKTEQMIKESVQ
                     SMKELAIGGTAVGTGLNAPAGFGDKVAEEISKAVGIEFTSALNKFHSLTSYDEVVYTH
                     GAIKALAADLMKIANDVRWLASGPRSGIGEIIIPANEPGSSIMPGKVNPTQSEALTMV
                     VAQVMGNDAAIGFAASQGNFELNVFKPVIIYNFLQSVTLLSDAMISFNDHCAVGIEPN
                     RDEIDRHVKNSLMLVTALNPYIGYENAAKIAKTAHEEGTTLKEAALKTGLLTEEQFDE
                     YVDPSKMIGK"
     gene            complement(1608..2534)
                     /locus_tag="VFX34_09910"
     CDS             complement(1608..2534)
                     /locus_tag="VFX34_09910"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016429636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX5565267.1"
                     /translation="MRKKKFHEIHSGLPTPLLRLGYAEVRLLGRWDGLSHALFSLHKG
                     RTFWVLYEHNDDIVETYTLLDGSVEMEYEGKVRMLEVGETIDASEYSNILTFKGITNT
                     ELLIKMTTTHFEPGFFETILLQEEADEIESVDGYTFMHSNRIKDYSIEVWKKLGLPKE
                     KLSILRWGAYFHDIGKRVVPLEILNKPGPLTPEEHEIMKAHTVKGAELMRNHEVKWLK
                     DAAFIVEQHHERFDGNGYPYGLKGDEISIEAAIVSVVDAFDAMTTDRVYRKSLSISDA
                     IQELIDGKGEQFHPRVVDAFLEVLQSESYKWK"
BASE COUNT          647 a          679 c          524 g          802 t
ORIGIN      
        1 ttttggatat acgcacatgt ttcattttcg aagataatgc gttgttccgg atcttcctct
       61 aacatgcaat atggacaatt tctcatcgat tgccgctcct tatttgccga tcatcttcga
      121 tggatcgaca tactcatcga actgttcttc cgtcagcaag ccggttttca acgcagcctc
      181 ttttaacgtt gtaccttctt catgcgcagt cttcgcaatc ttcgctgcat tctcatagcc
      241 gatatacgga tttaatgccg ttacgagcat caatgaattt ttcacatgac gatcaatttc
      301 atcacggttc ggctcgatgc caaccgcaca atggtcattg aagctgatca tcgcatcgct
      361 gagaagtgtc acggactgta ggaagttata gatgatgaca ggcttaaata cgttgagctc
      421 aaaattacct tggctcgccg caaagccgat tgccgcgtca ttccccatga cttgtgcaac
      481 aaccatcgtc aacgcttcac tttgggtcgg gtttacttta ccaggcatga ttgagcttcc
      541 cggctcgttt gcagggatga tgatttcgcc gattccagat ctcggaccac ttgccagcca
      601 acggacatca tttgcaatct tcattaagtc ggccgcgaga gctttaatcg ctccatgcgt
      661 gtacaccact tcgtcatagc ttgtcagtga gtggaattta ttcaaagcgg aagtaaactc
      721 gatgccgacc gctttactga tttcctcggc cactttatcg ccaaagccag ccggtgcatt
      781 taatcctgta ccaacggcag tcccgccgat ggcaagctcc ttcatagact gaacactttc
      841 cttgatcatc tgctccgtct tttccagcat acggtgccaa ccactaattt cctgaccaag
      901 tgtcagagga gttgcatctt gcaagtgcgt ccggccgatt ttaataatgt ccataaactc
      961 ttccgacttt tgtgcaagag tttcctttag cttgttaagc gcaggcagca actctgtttc
     1021 tactgcaatt acacctgcaa cgtggagggc tgtcggaaat gtgtcattgg agctttgaga
     1081 tttattgacg tcgtcatttg gatgtagacg ttcttcctct ccccactccg ccagtaattc
     1141 atttccacgg tgggcaatga cttcatttac gttcatattt gattgggtac cactaccagt
     1201 ttgccaaaca acgagtggga aatggtcgtc tagctttcca ctactaattt catccgcagc
     1261 tgctgaaatt gctttcattt ttgcttcaga aagatttcca aatgaatggc tcgcaattgc
     1321 cgcggacttc ttcagatggg cgaatgcgcg gattacacca atcggcatcc gctctccgcc
     1381 gattttaaag ttctgcttgc ttcgctgtgt ttgcgcgccc cataacttat ccgcgggtac
     1441 tttgatctca ccaattgtat ctcgttcaat acggtattcc atcgattctc caccctttca
     1501 tttgtttaaa aattgcatcc tctttaatga tgcatgaaaa caagggtaat taacaataaa
     1561 aagaaccgcc aatgtatgat cggcggctgc cctctcaact attccactca tttccacttg
     1621 tagctttctg actgaagcac ctccaggaat gcatccacga ctctcggatg gaactgctcc
     1681 cctttcccat caatcaattc ttggattgca tcagaaatag ataacgattt tcgatagacc
     1741 cgatccgtcg tcatggcatc aaacgcatcc accacagaca caatggctgc ctctattgaa
     1801 atctcgtctc cttttaatcc gtagggatat ccgtttccgt caaaccgctc atgatgttgc
     1861 tccactatga acgcagcgtc cttgagccat ttcacctcgt ggtttcgcat taattctgcc
     1921 cctttaaccg tgtgggcctt cataatttcg tgttcctcag gagtaagtgg tccgggctta
     1981 tttaagattt caagtggaac aacccgcttt ccaatgtcat gaaagtaggc accccatctg
     2041 agaatactaa gcttctcttt cgggagaccg agctttttcc atacttcgat cgaatagtct
     2101 ttaatccggt tactgtgcat aaatgtgtat ccgtcgaccg actcaatctc gtctgcttcc
     2161 tcttgtaata agattgtttc aaagaagcca ggttcgaaat gggtagttgt cattttaatg
     2221 aggagttctg tgtttgtaat ccccttgaat gtaagaatat tgctgtactc tgacgcatcg
     2281 atcgtttcgc caacctcaag catacgaact ttcccttcgt actccatttc gacagagcca
     2341 tccagaagcg tatatgtttc tacgatgtca tcattatgtt catataacac ccaaaatgtc
     2401 cgccctttat gcaaggaaaa tagtgcatgg gagagaccat cccaccttcc gagcaaacga
     2461 acttccgcat aaccaagccg taatagtgga gtcggaagac cactatgtat ttcatgaaat
     2521 ttctttttcc tcattatggg cccccgatcc gttgctcgaa tattgtcgaa attgtaatat
     2581 ttcaataatt atagcacgga tgagtcactc gtgaatacag attttttagg accaactcct
     2641 atgaacgagg ta
//
LOCUS       DASULI010000459         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_92472, whole
            genome shotgun sequence.
ACCESSION   DASULI010000459 DASULI010000000
VERSION     DASULI010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92472"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFX34_09915"
     CDS             <1..243
                     /locus_tag="VFX34_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017153888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX5565268.1"
                     /translation="FAQARWGATEGISNVQTLSDHRDLSFGEAYGVVMKELRLLARSI
                     FVVDKDDTVTYVEYVMEGTDHPNYEKAIAAVNELTK"
     gene            318..1235
                     /locus_tag="VFX34_09920"
     CDS             318..1235
                     /locus_tag="VFX34_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5565269.1"
                     /translation="MVTNTEQIFTFLNEYAESNKEKLYLEALIEACHEWLAEENMPRL
                     TPDVTKEEIRRGIQLAVLKGMKEQAQPNHQMTPDALGMLIGHFAGKLMQHKDQVTLLD
                     PAAGTGNLLFTVMNAIGDRATATSVEIDELLIRLSAVTADLLEQPVRFFIQDALRPLL
                     VDPVDLVVCDLPVGYYPDDENGINFEMMPAEGHAFSHHLFIEQSMNHLKEGGYALFLT
                     PAALFESEQSAELHRYLKKNAVIRAVLQLPASLFRSAAHEKSIFILQKPFAEMKAKPD
                     VLLSKVPSMSDKLAMARFIEQIDEWVAES"
     gene            complement(1536..1985)
                     /locus_tag="VFX34_09925"
     CDS             complement(1536..1985)
                     /locus_tag="VFX34_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010288291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5565270.1"
                     /translation="MVLKYDQIIVAVDGSKESRWAFKKAMGIAERNDATLNLVNIIDT
                     RSYAAVEAYDRSIAERAQKFAEELLTEYKQEAEAAGLHNVNIVVEYGSPKTMISRDLS
                     KKLQADLIICGATGLNKVERFLIGSVSENIVRSAACDVLVVRTPEEV"
     gene            2302..>2468
                     /locus_tag="VFX34_09930"
     CDS             2302..>2468
                     /locus_tag="VFX34_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5565271.1"
                     /translation="MRIGIPKEVKNNENRVAMTPAGAFNLKSAGHEVLIETGAGIGSS
                     FTDEDYIEAGAT"
BASE COUNT          713 a          533 c          573 g          649 t
ORIGIN      
        1 tttgcacaag caagatgggg tgcaacagaa gggatcagca acgtccagac attatcggac
       61 catcgggacc tttcattcgg ggaagcatac ggtgtcgtca tgaaagagct acggctattg
      121 gcgcgctcca tcttcgttgt cgacaaggac gatacagtca cttacgttga gtacgtaatg
      181 gaaggtacag accatcccaa ttatgaaaaa gcgattgcag cagttaacga actgacgaaa
      241 taattagctg gcccactcgg caacgggtgg gccacattac cgttaagaag gtatattttg
      301 gattagaggg atacgatatg gtgacaaata ctgaacagat ttttacattt ttaaatgaat
      361 acgcggaatc caataaagag aagctatatt tggaagcttt gattgaagcg tgtcatgaat
      421 ggttggcgga ggaaaacatg ccccgcttga ctcccgacgt gacaaaagag gaaatccgca
      481 gagggattca gcttgcagtt ctgaaaggaa tgaaagaaca agcacagccg aatcatcaaa
      541 tgacaccgga tgcacttgga atgctcattg gtcatttcgc tggcaagcta atgcagcata
      601 aagatcaagt gaccttgctt gacccagccg caggaacggg caatttgctg ttcaccgtca
      661 tgaatgcaat cggagatagg gcgacagcga catccgtgga aatcgatgag ctattgataa
      721 gattgtccgc tgtcacggct gaccttctgg aacagcctgt caggtttttc atccaagatg
      781 cattgcggcc tttgcttgtg gatccggtcg acttggtcgt atgtgacctg ccggtaggtt
      841 attatccgga cgatgagaac ggcatcaact ttgaaatgat gccagcggaa gggcatgctt
      901 tctcccatca tcttttcatt gaacagtcga tgaaccattt gaaggaaggc ggatatgcac
      961 ttttcctgac gcctgcagcc cttttcgaat cagaacaatc agcagagctt caccgctatt
     1021 tgaaaaagaa tgcggtcatc cgggccgtcc ttcaactgcc ggcatcctta ttccgaagcg
     1081 ccgcgcacga gaagagcatt ttcattttgc agaagccatt tgcagaaatg aaagccaagc
     1141 cggatgtgct gctgtccaag gtgccgagca tgtcggataa gctggcgatg gcacgcttca
     1201 ttgagcaaat tgatgaatgg gtcgccgaat cataaaagaa gaatttataa attaacccgt
     1261 tccaatccat ttgtggcttg gaacgggtat ttgtttgcat taaatggcat gccatataca
     1321 ggtctaaaat accgaaatga tgataacttt tgtgaagtga ccgataactc tgctggattg
     1381 accgataacc tatgggaagt gaccgatatc cgcgtggaaa tgatcgataa gtttcccggt
     1441 agtcccaaat actgctcaaa ccacatttga ctaaatcaaa aaagttcaaa gaaaatgaaa
     1501 aagaacctgc ccggtaaaag ggcaggttct tcactttata cttcttcagg tgtacggaca
     1561 acaagaacgt cacaagccgc agagcggaca atgttctctg acacgctgcc aatgaggaag
     1621 cgctccactt tgttcaaacc ggtggctcca cagatgatga gatctgcttg aagcttcttg
     1681 gacaggtcac gtgaaatcat cgtttttggc gaaccatatt cgacgacgat gtttacgtta
     1741 tgcaaaccgg cggcctctgc ctcctgttta tattctgtaa gcagctcctc agcaaatttc
     1801 tgagcacgtt ctgcgatgga acgatcatac gcttcgactg ccgcatagga acgtgtatcg
     1861 atgatattca ccaagttcaa tgtcgcatca ttccgctccg caatgcccat tgcctttttg
     1921 aacgcccatc ttgactcctt cgatccatcc acagccacga taatttgatc gtattttaat
     1981 accattccaa tcactccatc ctgtagattt gtatttacat ctacttactt cgataaaaat
     2041 cggaaaaaac ctttcttttt caaatgaatt caataagtaa ccgccggaat ttggcggaag
     2101 tgtgacagag gatattaatt tcattccctc tccattccta taaaaaacat gaaatgggta
     2161 atgaaatcct tcgatagtaa aattgtttat tatctttcta gagcaaattc ttataatttc
     2221 tgatagaatt gttagtacat tggtcttgtc gaattgtcga ttggtggcca actaactttc
     2281 ttatatatag gaggatcaga aatgcgtatt gggattccaa aagaagtgaa gaataacgaa
     2341 aatcgggtgg cgatgacacc tgcaggtgcg ttcaacttaa agtctgcagg ccatgaagtg
     2401 ttaattgaaa ctggtgcagg tattggttct agctttacag acgaggacta tatcgaggca
     2461 ggtgcgac
//
LOCUS       DASULI010000460         9079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1902820,
            whole genome shotgun sequence.
ACCESSION   DASULI010000460 DASULI010000000
VERSION     DASULI010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902820"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1166)
                     /gene="recA"
                     /locus_tag="VFX34_09935"
     CDS             complement(102..1166)
                     /gene="recA"
                     /locus_tag="VFX34_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5565272.1"
                     /translation="MSDRKAALDQALKQIEKQFGKGSVMKLGEKTDREIATSSTGSLA
                     LDAALGVGGYPRGRVIEIYGPESSGKTTVALHAIAEVQAGGGQAAFIDAEHALDPVYA
                     QKLGVNIDELLLSQPDTGEQALEIAEALVRSGAIDIVVIDSVAALVPKAEIEGEMGDS
                     HVGLQARLMSQALRKLSGAINKSKTIAVFINQIREKVGVMFGNPEVTPGGRALKFYSS
                     VRLEVRRGEAIKQGNDIMGNKTRIRVVKNKVAPPFRTAEVDIMYGEGISREGEIVDLG
                     ADLEVVQKSGAWYSYEGERLGQGRENAKQFLKENPDIRAEIANKIRDSYGMAAANYVI
                     AGHDEDDEEELDLLLLEDEK"
     gene            complement(1342..2595)
                     /locus_tag="VFX34_09940"
     CDS             complement(1342..2595)
                     /locus_tag="VFX34_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX5565273.1"
                     /translation="MKAEIIAVGSELLLGQITNTNAAFISARLAEVGIDVYYHTVVGD
                     NPKRLEDAITIAQNRADLIIFSGGLGPTKDDLTKETIAKMLGTELESDNAALSSIEAY
                     FNRTGRSMTENNKKQALILTGSTVLTNNNGMAPGMAIEKNGICYILLPGPPHEMEPMF
                     EKEAIPYLLGTSGKREVIVSRVLKFYGIGEAELEYRVQSILEKQTNPTVAPLASPDSV
                     ILRLTAKAESNEAAQKLIEPVESEIRSMVGPFIYGTDDDTLSSKAAELLKCSKFTISA
                     AESLTAGLFMAELADEPGISSSLRGGVVVYDEAAKMDQLDIPGSLLNEYGVVSPECAA
                     VLARNVKRKFGSDIGVGLTGAAGPDPHDGKPVGTVWIGIAFRDEEVKTYKLDLSGSRN
                     ANRKRAARFALYYLIQEMKRQNQEN"
     gene            complement(2608..3186)
                     /gene="pgsA"
                     /locus_tag="VFX34_09945"
     CDS             complement(2608..3186)
                     /gene="pgsA"
                     /locus_tag="VFX34_09945"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX5565274.1"
                     /translation="MNLPNKITVSRIVLIPIFIIFMVVDFGFGSFEIGGTTLSVEQLI
                     GALLFIIAAATDWFDGHLARKHNLVTNMGKFLDPLADKLLVSAALIILVEMGSAASWI
                     VIIIISREFAVTGLRLVLAGGGEVVAANQLGKIKTATQLVAIAFLLLNNIFFEAINFP
                     FGTIMLYIALFFTIWSGADYFYKNRRVLLDSM"
     gene            complement(3242..4114)
                     /locus_tag="VFX34_09950"
     CDS             complement(3242..4114)
                     /locus_tag="VFX34_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5565275.1"
                     /translation="MTGLGDRLKEARTAKGYSLDDLQSITKIQKRYLSGIENEDYSMM
                     PGAFYVRAFIKQYSEAVGLDSDEMLSLYKESAMHEKLEEENAQHTPPMKRSRGIGNSR
                     LNELFPKLIVALFIIVIIVVIWTFFQKNASNKTGMDVEPDNPITMDSNQPPAGNVDGG
                     AEDADSGSDVEESTEPDPETEEDVKEQTLSYVNTIGEDSTYTLTGADEFKLKIDTFGD
                     TWIGVQNENKEELTPAARVMKNGETIELDVSDVEFVRIRVAVTPYTDIYVNGEKLEYV
                     SDKSTQNIFIEYEK"
     gene            complement(4132..4929)
                     /locus_tag="VFX34_09955"
     CDS             complement(4132..4929)
                     /locus_tag="VFX34_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017380892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HEX5565276.1"
                     /translation="MGEWYVEYEIQVNRPGLLGDIASLLGMLRVNIVTINGVDGGHRG
                     MLVRTENDDQIKRFELIASTMETIQVKKIREPKLRDILAVRHGRYIQRDVDDRKTFRF
                     IRSELGVLVDFMAELFKQEGHKLIGIRGMPRVGKTESVVAASVCANKKWIFLSSTMIK
                     QTVRNTLAGDEFSKDNIFILDGIVTRKSGDERHMQLVREMMSMPSIKVVEHPDMFVQH
                     SEYDMEDFDYIIELRTDPDQEITYEMMEKNHMMSNMGPMGGFGTFNF"
     gene            complement(5104..5832)
                     /locus_tag="VFX34_09960"
     CDS             complement(5104..5832)
                     /locus_tag="VFX34_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5565277.1"
                     /translation="MKRYAVVLGSSGGIGEAVSRKLAADGWSLYLHYNSNVVRASELK
                     RILTEKYPEADFQLVQSDMAELDGAAKLAEQVGHVQAIVVASGQSMLKLLTDTTRSDM
                     EALWRVHMQNPAMFISHASPQLRSHPVSYVVFIGSIWGNTGAAGEVMYSAVKGAQHAF
                     VKAYAKEAAYTGTRVNAIAPGWIETDMNGELTEEDKQLVIDQIPLQSTGQPEQVADLA
                     SFLLSGKADYMTGEVLKLNGGWYI"
     gene            complement(5835..7133)
                     /locus_tag="VFX34_09965"
     CDS             complement(5835..7133)
                     /locus_tag="VFX34_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5565278.1"
                     /translation="MEKITFENLQETLFHERLDNGLHVYILPKRGFSKTYVTFTTKYG
                     SIDRTFIPRGKNEFITVPDGIAHFLEHKMFDKKEGDVFQKFGVLGASANAFTSFTRTS
                     YLFSTTDNLYENTKVLLDFVQEPYFTEEKVEKEKGIIAQEITMYDDNPDWRNYFGTIQ
                     SLYKEYPINIDIAGTVDTIQEITAEHLYECYETFYHPSNMTVFVIGAVDPEEMMNFIR
                     EDQNSKEFSEPQEIKRKYPEEPVEANRKKTVLEMDVLRPKFNYGMKCNRTVMEGQEML
                     RQDLAVNLLLDILFGRTSEFYTKAYNDHLIDETYHYEFISEKDYGFAMVESDTEKPAE
                     LEAAVRSALKSLAADWSITEDDLNRARKRRIGQFMRSLNSIEFISNQFTQYNFNEMNL
                     FDVVPTLEKIEMGQLQDAFKTISNEESHTVMTIVPGSKEG"
     gene            complement(7136..8401)
                     /locus_tag="VFX34_09970"
     CDS             complement(7136..8401)
                     /locus_tag="VFX34_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5565279.1"
                     /translation="MFTKVKLQKGVNLYIRKTDQFKTITMSIKWLAELDEKQAAYRAV
                     LSNVLQDSNADYPKQSDLRKALDELYGTVFYMDAGKRGNSHIVSLNMECVNDEYLSTS
                     GVFDEALKMIHSVIFKPNLKDGSFEEAIVNREKRTVIERIRSIYDDKGRYAHKRMLEL
                     MRPNDPASMSASGTEEDVESITNAILLDTYKRMLDEDEIDIYVVGDVQEDELTEKIKS
                     MFEFVERAVTKKQYEQPDKEVKEARQVFERQDMKQGKLQVGYSTPVSFFHPDYPKMQV
                     ANGVLGGFAHSKLFMNVREKESMAYTVNSAYAAYYGILYVMAGIDAELEEKAVKLIGE
                     QIDEMQNGNISDLELDQTVALLRNSIRSAFDSARGQIEVYDQYKQLDEDFTADKWIAK
                     WEAVSIEDVKQMASQIHLELVYLLSGREA"
     gene            complement(8777..>9079)
                     /locus_tag="VFX34_09975"
     CDS             complement(8777..>9079)
                     /locus_tag="VFX34_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5565280.1"
                     /translation="QTMTGDFGHATINGQGFMALAAMIFGKWHPIGAMGAALFFGFAQ
                     ALAISAGSIEIDFIQKIPTVYFHILPYVLTILALAGFIGKANAPKANGIPYIKGSR"
BASE COUNT         2264 a         2331 c         1846 g         2638 t
ORIGIN      
        1 cgtatttctg cgggttttgc agaaatcaag tcagcccccc ggagtctaga cggaaacaaa
       61 agcggactgc cattataaaa ggcagtccgc tagtcaatca attatttttc atcttctagc
      121 agcaataggt cgagttcttc ctcgtcgtct tcatcatggc ctgcgatgac gtagtttgcg
      181 gcagccattc cgtaggagtc acggattttg tttgcgattt ctgcgcggat atccggattc
      241 tccttcagga attgcttagc attctcacgt ccttggccga gacgctcccc ttcataggaa
      301 taccatgcgc cgctcttttg tacaacttcc agatcggctc cgaggtcgac gatttcccct
      361 tctctggaaa tcccttcacc gtacatgata tccacttccg cagtacggaa cggaggagca
      421 accttgtttt tcacgacacg gatgcgtgtt ttgttaccca tgatgtcgtt tccttgttta
      481 atagcttcac cacgacgcac ttccaaacgg accgacgaat agaatttcaa tgcccgtccg
      541 ccaggtgtca cttcaggatt cccgaacatg acgccgacct tctcacggat ttggttgatg
      601 aagacggcaa tggtcttcga tttattgata gcgcctgaca gtttacgaag agcttgggac
      661 atgagacgtg cctgcaagcc gacgtgcgag tcgcccattt ccccttcgat ttccgctttt
      721 ggcaccaatg ctgcaacaga gtcgatgaca acaatgtcaa ttgcaccact tctaacgagt
      781 gcttcggcga tttcaagcgc ctgttcgcct gtatccggct gtgacaatag caactcgtca
      841 atattgacgc ccagtttctg agcatatacc ggatccaatg cgtgctctgc atcgatgaaa
      901 gcggcttgtc cgcctcccgc ctgcacttca gcaattgcat gcaatgccac tgtcgtctta
      961 cctgaacttt ccggtccgta gatttcaatg acacgacccc ttggatatcc gccgactcct
     1021 aatgccgcat ccagtgccag cgagccggta gaagacgttg caatctcccg gtcggtcttt
     1081 tcccccaatt tcatgacgga ccctttaccg aattgttttt caatttgttt taaagcttgg
     1141 tccaaagctg ctttacgatc gctcaaaaac gattcctccc ttaaaaggcg ttattctttt
     1201 tcttcctgtc tctactatac tccttttatg agaaatactc aaacaaaaaa cgaacgctta
     1261 ttcgatttat tttatcaaaa atcattttta attgagaatg aggggggaaa atcaatttta
     1321 aatgagaata ggaggcaaaa attaattttc ctgattctgt ctcttcatct cttgaattaa
     1381 ataatagagt gcgaatctgg ccgcccgttt ccgattcgca tttcttgaac ctgaaagatc
     1441 aagtttatag gtcttcactt cttcatcccg gaaagcaatt ccgatccaaa ctgtcccgac
     1501 cggcttcccg tcatgcggat ccgggcccgc agcaccggtc aatccgacac cgatgtccga
     1561 accgaatttc cgtttcacat tgcgagccaa cactgcagcg cattccgggc tgacgacacc
     1621 gtattcattc aataatgaac ctggaatatc caactgatcc atcttcgcgg cctcatcata
     1681 gacgaccact cctcctctca gggaagagct gatgcctggc tcatccgcca attcggccat
     1741 gaataagcct gccgtcaagc tttcggccgc agagatggtg aacttgctgc atttcaatag
     1801 ttcagccgct tttgaagaca aagtgtcatc atctgtgcca tagatgaatg gaccgaccat
     1861 tgaccggatt tccgattcca ctggttcaat caacttttga gccgcctcat tcgattcggc
     1921 tttcgcggtc aatcgcagaa tgaccgaatc aggtgaagcc aaaggggcga cagtcggatt
     1981 cgtctgcttc tccaagatcg attggacacg atactccagt tcggcttcac caattccata
     2041 aaacttcaac acgcgggaaa cgatgacttc acgtttgcct gacgtcccca ataaataggg
     2101 aatcgcttct ttttcaaaca tcggttccat ttcatgggga ggtccgggca ataaaatata
     2161 acatatccca ttcttttcga tagccatacc tggcgccatc ccgttattgt tcgttaaaac
     2221 tgtcgaaccg gtgaggataa gcgcctgctt cttattgttt tcagtcatgc ttctgccagt
     2281 ccgattgaag taggcttcga tggatgacaa tgcggcattg tcgctttcaa gttcagtgcc
     2341 aagcatcttt gcaatggttt ctttcgtcaa atcatccttc gtcggtccca atcctccgga
     2401 aaagatgatc agatcggcgc ggttttgcgc aatggtgatc gcatcttcaa gtcttttcgg
     2461 gttgtccccg acgaccgtat gataatagac atcgatgccg acttcagcaa gacgtgccga
     2521 aatgaatgct gcgtttgtgt tcgtgatttg tcctaatagg agttctgacc ctactgcgat
     2581 aatttccgct ttcattatgc catctcctta catcgagtcg agtaagaccc tccggttttt
     2641 atagaaataa tcggcccccg accagattgt aaagaacagg gcgatataga gcatgatggt
     2701 gccaaatggg aagttgattg cttcaaagaa aatattattt aatagtaaga aggcgattgc
     2761 aaccaactga gttgccgttt taatcttccc taattggttc gcagccacga cttcaccgcc
     2821 acccgccagc actagcctca atccggtaac agcaaactcc ctgctgatga tgatgatgac
     2881 gatccatgac gcggccgaac ccatttccac taaaataatc agtgcagctg atacaagaag
     2941 cttatcagcg agcggatcca ggaatttccc catgttcgta accaaattat gttttctcgc
     3001 caaatgacca tcaaaccaat ccgtcgcggc cgcgatgatg aataacaacg caccgatcaa
     3061 ctgctccacg gagagcgtgg tgcctccgat ttcaaaagat ccaaagccga aatcgacaac
     3121 catgaagata atgaatatcg ggatgagcac aatgcgggat actgtaattt tattaggtaa
     3181 attcattcgt ctcacttatc cttccggaat gaaaaaagtc atctttgtag atgactattt
     3241 ttcatttctc gtattctata aaaatgtttt gcgtcgactt atctgataca tactccagtt
     3301 tttcaccatt tacatagatg tcggtatatg gagtcacggc aacacggata cggacaaatt
     3361 caacatctga aacatccagc tcaatcgttt cgccattttt catgactctt gcagccggtg
     3421 taagttcttc tttgttctca ttctgtacgc cgatccaagt atctccgaaa gtatcgatct
     3481 ttaacttgaa ttcatctgca cctgtcaatg tgtatgtcga atcttcccca attgtattta
     3541 catacgataa ggtctgctcc ttcacatctt cctcagtttc aggatccggc tcagtcgatt
     3601 cttcaacgtc agagccagaa tcagcgtcct ccgccccacc gtcgacgttg cctgccggcg
     3661 gctgattgct atccatcgtt atcggattgt ccggctctac atccattccg gttttattgg
     3721 acgcgttttt ttggaagaat gtccatataa caacaatgat gacgataatg aataatgcca
     3781 caataagttt cgggaacaat tcattcagac ggctgttgcc tattccgcgg ctcctcttca
     3841 ttggaggggt atgctgagca ttctcttctt cgagtttctc atgcatggcc gattctttat
     3901 acaaagataa catttcgtct gaatctaaac ctacagcttc cgaatactgt ttaatgaaag
     3961 cacgtacata gaaagcgccg ggcatcatgc tgtaatcttc attttcaata ccggaaaggt
     4021 accgtttttg aattttagtt atcgattgta aatcatccag tgaatatccc ttagctgttc
     4081 tcgcctcttt aagacgatca cctaatccgg tcaattttat cacctgccta tttaaaagtt
     4141 gaacgtacca aatccgccca tcggtcccat gttggacatc atatggtttt tctccatcat
     4201 ttcatatgta atttcctggt ccggatctgt ccgtaattcg ataatataat caaaatcctc
     4261 catgtcgtat tcagaatgct gaacgaacat gtccgggtgc tcgaccactt tgatggaagg
     4321 catgctcatc atttcacgga caagctgcat atgcctttca tcgcccgatt tgcgagtgac
     4381 aattccatcg aggatgaaaa tattgtcctt cgagaattca tcgcccgcca gcgtatttcg
     4441 cacggtctgt ttgatcatcg tggaagaaag gaatatccat ttcttatttg cacacacgct
     4501 tgccgcaacg actgattccg ttttaccgac ccgcggcatc ccccgaatcc cgatcaattt
     4561 gtggccttcc tgcttgaaaa gctccgccat gaaatcgaca agtacgccca attcgctgcg
     4621 aatgaatctg aatgttttcc ggtcatcgac atcgcgctgg atatagcgcc catgtcggac
     4681 cgccaaaata tcacgcaatt tcggttcacg gatttttttc acctgaatgg tctccattgt
     4741 ggaggcaatc aattcgaaac gcttgatctg atcgtcattt tcagtcctga caagcatacc
     4801 gcgatgtccg ccatccacac cgttgatggt tacgatattg acgcgcaaca tcccgagtag
     4861 ggatgcaatg tctccgagca ggcctggccg attcacctgt atctcatatt cgacatacca
     4921 ttcacccatt cgacacaccc tttcgaacca ttttcaccca aagtgatttt gattatacgg
     4981 ccccacctgg gagcacttca attagtagga cgcataagcc gcaccttagt tctataatag
     5041 cggattcgac agctgaagaa aaggaaaaaa gaaaaagggg aaaattgtct ccccaatctc
     5101 tggttatata taccatccgc cattcagctt caaaacttcc cccgtcatat aatccgcttt
     5161 tccgcttaga aggaaactgg caagatcagc cacttgttcg ggctggcctg tcgactgcaa
     5221 agggatttga tcaatgacga gttgcttatc ttcttccgtt aattccccat tcatatccgt
     5281 ttcaatccat ccgggggcaa tggcattcac tcgcgttccg gtataagccg cctcttttgc
     5341 atatgctttg acgaatgcat gctgggctcc tttgacagcc gaatacatca cttccccggc
     5401 agcacctgta ttgccccaaa tcgagccgat gaacacaaca tatgaaacgg ggtggctccg
     5461 cagctgcggc gatgcatggc taatgaacat tgctgggttt tgcatatgta cacgccataa
     5521 cgcttccatg tcgctacgag tcgtatccgt caataatttc aacatcgatt gaccggatgc
     5581 gacgacaatc gcttggacat gtccgacttg ctccgccagc ttcgctgccc cgtccagttc
     5641 cgccatatcc gactggacaa gctggaaatc cgcttccgga tatttttcag tcaatatcct
     5701 tttcagttcc gaagcccgca ctacgttact gttgtaatga agatacaagg accatccatc
     5761 tgcggccagc ttacggctga ccgcttcccc gatacctccg gatgaaccta ataccactgc
     5821 atagcgcttc attctcagcc ttcttttgag ccggggacaa tcgtcatgac agtatggctt
     5881 tcttcattgg aaatcgtctt gaatgcatcc tgcaactgcc ccatttcaat cttttcaagt
     5941 gtcggcacca catcgaataa attcatttca ttgaaattgt attgtgtaaa ctgattggaa
     6001 atgaattcga tagagttcaa cgaacgcatg aattgaccga ttctacgctt tcttgcacga
     6061 ttcagatcat cttcggttat cgaccaatca gccgccagcg atttcaaggc agagcggact
     6121 gcagcttcca attcagctgg cttctcagta tcagattcga ccatggcgaa cccgtaatct
     6181 ttctcggaaa tgaactcata gtggtatgtt tcatcgatca aatgatcatt atacgctttc
     6241 gtataaaatt cggaagtcct gccgaataaa atatcgagaa gaagattgac cgccaaatcc
     6301 tggcgaagca tttcctgacc ttccatcacg gttcgattgc atttcatgcc ataattgaat
     6361 ttcggtcgca gcacatccat ttccagaacc gttttcttcc tgttcgcttc tactggctct
     6421 tcaggatatt tgcgcttgat ttcttgaggc tcggaaaatt ccttgctgtt ttgatcttcg
     6481 cggataaagt tcatcatttc ctctggatcg acagctccaa tgacgaagac agtcatgttc
     6541 gaagggtgat aaaatgtttc gtagcactcg taaagatgct ccgcagtaat ttcttggatt
     6601 gtgtccactg ttcccgcaat atcgatattg atgggatact ctttgtacaa gctttgaatc
     6661 gtgccgaaat aattacgcca atccggatta tcatcataca tcgtgatttc ctgcgcgatg
     6721 atgccttttt ccttctccac tttttcttct gtgaaataag gttcttgtac gaaatcaaga
     6781 agtactttgg tgttttcata taagttgtcg gtcgtggaaa acagatagga cgtacgcgta
     6841 aacgaagtga atgcgttcgc agaagcgcct aggacaccaa acttctggaa aacatcccct
     6901 tccttcttgt cgaacatttt atgctctagg aaatgggcga ttccgtcagg caccgtaata
     6961 aattcgtttt taccccttgg aatgaacgtc ctgtctattg aaccatattt cgtcgtaaac
     7021 gtcacatacg tcttcgaaaa gccgcgctta ggcaaaatat acacgtgcag gccattatca
     7081 agcctctcat ggaatagtgt ttcctgtaag ttttcaaatg tgatcttttc catcattacg
     7141 cctctcttcc cgacaacaag tagacgagtt ccaggtggat ttgggatgcc atctgtttaa
     7201 catcttcgat tgagaccgcc tcccatttgg caatccattt atctgctgtg aaatcctcat
     7261 ccagttgttt atattgatcg tacacttcga tttggcccct tgccgaatcg aatgcgctgc
     7321 ggatgctgtt tctcaacagg gcaactgtct gatcaagctc caaatcggat atgtttccgt
     7381 tttgcatttc atcaatctgc tcaccgatca gtttgacggc tttctcttca agttccgcat
     7441 caattcctgc catgacatat agaatgccgt aatatgccgc gtaggcactg ttgacagtgt
     7501 atgccatgct ttctttttcc cggacgttca tgaacagctt gctatgtgcg aaaccgccca
     7561 acacaccatt ggccacttgc attttaggat agtcgggatg gaagaaggaa accggtgtgc
     7621 tatagccgac ttgcagcttc ccttgcttca tgtcctgcct ttcaaatact tggcgggcct
     7681 ctttgacttc cttatcaggt tgttcatatt gtttctttgt gacggctctt tctacaaact
     7741 cgaacatcga cttgattttt tctgtgagtt cgtcctcctg gacatctccg acaacataaa
     7801 tgtcgatttc atcttcatcc aacattctct tgtatgtatc aaggaggatc gcattcgtaa
     7861 tactttcaac atcctcttcg gtaccgcttg cagacatcga cgctggatca ttcggacgca
     7921 tcagttcaag catgcgctta tgggcatatc ttcctttgtc atcataaatg gaccggatcc
     7981 tttcgatcac tgtccgtttt tcccggttca cgattgcctc ttcgaatgat ccatccttta
     8041 aattcggttt gaagatgacg gagtggatca ttttcaatgc ctcatcaaaa acacccgacg
     8101 tggataggta ttcatcattc acacattcca tattcaagga tacgatgtgt gagttgcccc
     8161 tctttcccgc atccatataa aagacggtgc catataattc atccaatgct tttcgcaaat
     8221 cgctctgctt tggataatca gcgttcgaat cttggagtac atttgataaa accgcccggt
     8281 atgctgcctg tttttcatcc agttcagcca accatttgat ggacatcgtt atcgtcttaa
     8341 attgatccgt tttgcggata tataaattga ctcctttttg aagcttgacc tttgtaaaca
     8401 tacgaacacc tcgtttttcc tttattatac gaataccctt ctacaatata catttcatcc
     8461 tatgcacaag gcaaaataaa cgcccatata tacaaaagcg gaggatgtct gatcattgct
     8521 cgtgacgctt tgcttttact cgcaaaagcc cttgttcgtt acgtctttag ctgcgacaca
     8581 taaggcagat ctgtgaaacg acgctttttg ccgtgtagcg gagctgactt atgacccgag
     8641 cgaatcgaac tgcagagtgt tcgatttgcc gtatttctgc ggtttttaaa gaaattaagg
     8701 cagcctttct gccatccgga gctagacgta aaaccgcctg gacccgaaga gcgggaatcc
     8761 aggcggtgaa agatcattat ctgctacctt tgatataagg aataccattc gctttcgggg
     8821 cattcgcttt tccgatgaac ccagctagtg caaggattgt cagaacgtac ggcaagatgt
     8881 ggaagtaaac ggtcgggatc ttctgtatga aatcgatctc gattgaacct gcactgatcg
     8941 ccaacgcttg ggcaaacccg aagaaaaggg ccgcacccat tgcaccgatc ggatgccatt
     9001 taccaaaaat catcgctgcc aacgccatga atccttgacc attgattgtc gcatgtccga
     9061 aatccccggt catcgtttg
//
LOCUS       DASULI010000461         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_235386, whole
            genome shotgun sequence.
ACCESSION   DASULI010000461 DASULI010000000
VERSION     DASULI010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235386"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..658)
                     /gene="wrbA"
                     /locus_tag="VFX34_09980"
     CDS             complement(50..658)
                     /gene="wrbA"
                     /locus_tag="VFX34_09980"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX5565281.1"
                     /translation="MADVKAAIIYYSSTGTNYQLAQWAAEGAKAAGAEVKVLKVPETA
                     PQAAIESNPAWKAHVDATTDVPEVTLQDLEEADAIIFSVPTRFGNVPSQVKSFLDTTG
                     GLWFHGKLANKAVSAMASAQNAHGGQEATVLALYTTMYHWGAVVVAPGYTDQSAFTSG
                     GNPYGTSVTVDQDGNMVENVQDAVKHQANRTIQVAKALQQMK"
     gene            complement(720..1328)
                     /locus_tag="VFX34_09985"
     CDS             complement(720..1328)
                     /locus_tag="VFX34_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5565282.1"
                     /translation="MKHIYKEGKDKTKPTLLLLHGTGGTEQDLLPLAEMIDPDASVLS
                     VRGNVLENGMPRFFKRLAEGVFDEEDLVFRTIELNGFLDEAAGKYGFDRRNIVAVGYS
                     NGANIAASLLFHFNDSLKGASLHHPMVPRRGIDLPNLDGVSVFISAGKNDPICPASES
                     EDLEAFLLGAGASVRMHWEMNGHSLTHTEVRAAANWYEENFS"
     gene            complement(1478..>2878)
                     /locus_tag="VFX34_09990"
     CDS             complement(1478..>2878)
                     /locus_tag="VFX34_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016115578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX5565283.1"
                     /translation="QFYASTNPEPVSSPSLVVFNDKVAESLGLNADALREDGAEIFAG
                     NQIPEGTTPIAQAYAGHQFGHFTMLGDGRAVLLGEQVTPGGKLMDIQLKGSGRTPFSR
                     GGDGRAALGPMLREFIISEAMHALGIPTTRSLAVVETGDPVYRETVLPGAILTRVASS
                     HVRVGTFQYAAARQVVEDVRALSDYAIERHYPEVADDQNPYLSLLEKVIDRQASLIAK
                     WQLVGFIHGVMNTDNMTISGETIDYGPCAFMDTYQPATVFSSIDREGRYAYVNQPGIG
                     AWNLARFAETLLPLFHQDMEQAVKIAQEKIGQYGKRFDAYWLDGMRRKLGLFNAEDGD
                     EELVHELLKLMEAFHADFTNTFRALTLDGVKKPDVFYSTQFKEWEAKWHERLSRQPQS
                     MEDAKQLMRDHNPSVIPRNHRVEEALEAAVEEGDLTVLERLLQVLAEPYAHTPEQEEY
                     ATPSTSTEPYRTYCGT"
BASE COUNT          689 a          822 c          604 g          763 t
ORIGIN      
        1 gttttctata ccccaaaaat aaaaaccgac ccatcggatc ggttctctct tacttcattt
       61 gctgaagtgc ttttgcaact tggatcgtac gattcgcttg gtgctttaca gcgtcctgta
      121 cgttttctac catgttgccg tcttggtcta cggtgacact tgtgccgtat gggtttccgc
      181 cagatgtgaa tgcactttgg tctgtgtagc ccggagctac aaccaccgca ccccagtgat
      241 acatcgttgt atataaagcg agtaccgtcg cctcttgtcc accgtgagca ttttgcgcgg
      301 atgccatcgc actcaccgct ttatttgcaa gctttccatg aaaccagagg ccgcctgtcg
      361 tgtctaaaaa gcttttcacc tgtgacggta catttccgaa tctcgtcggc acactaaaga
      421 taatcgcgtc cgcctcttcc aaatcctgta gtgtcacttc cggaacgtcc gttgtagcat
      481 ccacatgtgc cttccatgct ggatttgatt caattgctgc ctgcggggct gtctctggca
      541 ccttcagtac tttcacttct gctcccgctg cttttgcgcc ttcagccgcc cactgggcaa
      601 gttgatagtt cgttccggtt gaactgtagt aaatgattgc tgcttttaca tccgccatgc
      661 tgctctactc ctttgttgtc aaatctaaga gcagtgttcc tcattggcga ttgtttcatt
      721 catgaaaaat tctcttcgta ccagtttgcg gccgctctta cttcagtgtg agtcaaggag
      781 tggccattca tctcccaatg catacggaca gacgcaccgg ctcccaagag gaacgcctcc
      841 aaatcttcgg attcacttgc agggcaaatc ggatcatttt tacctgcgga tataaaaact
      901 gataccccat caagattcgg caaatcaatc ccacgacgtg gcaccatcgg atgatggaga
      961 cttgccccct tcaacgagtc attgaaatgg aaaagaaggc ttgctgcaat gttcgctccg
     1021 ttcgagtagc caactgccac aatgttccgg cggtcaaacc catacttgcc cgctgcctcg
     1081 tctaagaatc cattcagttc aattgtgcgg aaaacgagat cctcttcatc aaaaactcct
     1141 tcagccaaac gcttaaaaaa acgcggcatc ccattctcga gtacatttcc tcgtacgctc
     1201 aacacggaag catctggatc aatcatctct gcaagcggca atagatcctg ctctgttcca
     1261 cccgttccgt gcaataataa gagcgtcggc tttgttttat ctttgccctc tttatagata
     1321 tgtttcatcg gtcatctctc ctttaattat ctcgaattaa agataaatgt aacagtggag
     1381 gattcataag tcaaaaagac agattgctaa ataaactcct ccgacccggc cttgttaccc
     1441 ccatcataca aagaaagcag gcacccgccc gaaaaggtta ggtgccgcaa tacgtgcgat
     1501 agggctcagt tgaagtcgaa ggcgtcgcat actcctcctg ttctggtgta tgcgcatatg
     1561 gttccgctag tacctgcaac aggcgttcca atactgttag atctccctct tccacagcag
     1621 cttccaacgc ttcttctacg cgatgattcc gcgggatgac actaggattg tggtctcgca
     1681 tcaattgctt ggcatcttcc atggactgtg gctgtctcga caaccgctcg tgccacttcg
     1741 cttcccactc cttgaactgg gtggaataaa agacgtccgg tttctttaca ccgtcaagcg
     1801 ttaaggcccg gaacgtattt gtaaagtctg catggaatgc ttccattaat ttcagaagct
     1861 catgcaccaa ttcttcgtcc ccgtcctccg cattgaagag cccaagtttc ctgcgcatcc
     1921 catccaacca atacgcatcg aatcgtttgc cgtattggcc aatcttctcc tgagcaattt
     1981 tcaccgcctg ctccatatcc tgatggaata aaggcaagag cgtctccgca aaccgcgcaa
     2041 gattccaagc gccaattccg ggttgattca catacgcgta ccgcccttca cggtcaatcg
     2101 agctgaacac agtggctggc tgatacgtat ccataaacgc acacggacca taatcaattg
     2161 tttctccgct gatcgtcata ttgtccgtgt tcatgacgcc atgaataaaa ccaacgagct
     2221 gccattttgc aatcaaggac gcctgcctgt cgattacctt ttcaagcaac gataaatacg
     2281 gattctggtc gtccgctact tccggataat gtcgttcaat cgcatagtct gataaagctc
     2341 ggacatcctc gaccacttgc cgagcagctg catactggaa cgtacctacc cgaacgtggc
     2401 tggaagcaac acgcgttaaa atggcacccg gcaaaacggt ctcccggtat accggatcac
     2461 ccgtttccac gaccgcgaga ctccgtgttg tcggaatccc aagtgcatgc atcgcttcac
     2521 taataataaa ttcccgcaac atcgggccaa gcgcagcccg tccatcgcct cctcgagaaa
     2581 atggagtccg gcctgatccc ttcaattgaa tatccataag ctttccaccc ggggtcactt
     2641 gttcaccaag caggaccgct cgtccatcac cgagcattgt aaaatgccca aattgatgac
     2701 cagcatacgc ttgcgcaatc ggcgttgtcc cctctggaat ttgattcccc gcaaagattt
     2761 cagcaccatc ttcccgtaac gcgtctgcat tcaaaccgag ggactccgca actttgtcat
     2821 tgaatacgac gagtgaagga gacgaaaccg gttccggatt cgtacttgca taaaattg
//
LOCUS       DASULI010000462         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_909192, whole
            genome shotgun sequence.
ACCESSION   DASULI010000462 DASULI010000000
VERSION     DASULI010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909192"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /gene="argF"
                     /locus_tag="VFX34_09995"
     CDS             complement(<1..801)
                     /gene="argF"
                     /locus_tag="VFX34_09995"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5565284.1"
                     /translation="MHISTLKTAKKIEGQLKGRDLLSLVDYTSEEIDYLLQKALVMKK
                     DARAGKIPPILQGKTLGMIFEKHSTRTRISFEVGMVQLGGHAMYMNSNDLQIGRGESV
                     YDTGNVLSGYLDAVMIRANSHEMVKELAKHSSIPIINGLTDMFHPCQALADLLTMIEV
                     KGTVAGKKLAYVGDGNNVAHSLAIGAAHMGMHVAIATPKGFEFNPNLFVEAKKIASAN
                     GGSITQTYDPVKAVKDADVVYTDVWASMGQEEEAAARLESFKGFQINDA"
     gene            complement(1037..2068)
                     /locus_tag="VFX34_10000"
     CDS             complement(1037..2068)
                     /locus_tag="VFX34_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEX5565285.1"
                     /translation="MPGNEREPREVMKKYIEPFADEIEYDGLGSLIAKKSGDANGPKV
                     MVAGHLDEVGFMVTKIDDKGFISFQPVGGWWSQVMLAQRVTIVTRKGDTVTGIIGSKP
                     PHILSAEARKKPVDIKDMFIDIGATSKEEAMEWGVLPGDMIVPYFEFTVMNNDKYLLA
                     KAWDNRIGCAIAIDVMKALKDENHPNIVFGVGAVQEEIGLRGAKTAAAKVKPDIGFAV
                     DVGVAGDTPGVTSKESTSKMGDGPQILLYDASMVSHKGLRDLVVDTAEEHGIPYQFEA
                     IPGGGTDAGSIHVSGNGVPSLAICIATRYIHSHAAMLHRDDYENAVKLIVEVIKKLDR
                     ETVDNIIFQ"
BASE COUNT          522 a          526 c          412 g          609 t
ORIGIN      
        1 agcatcgttt atttggaacc ctttgaacga ttcaagacgt gcggccgcct cttcttcctg
       61 gcccatgctt gcccagacat cagtatagac gacatccgca tctttcacag ctttcactgg
      121 gtcataggtc tgcgtgatag atccgccatt ggccgaagca atcttttttg cttccacaaa
      181 taggttcgga ttgaactcga accctttcgg agttgcaatc gctacatgca ttcccatatg
      241 cgctgcacca atcgccaagg aatgggcgac attatttccg tcgccgacat atgcgagttt
      301 tttgcctgcg acagttccct tcacttcgat catcgtcagt aaatccgcaa gcgcctggca
      361 aggatgaaac atgtcggtca gtccattgat gataggaatg gaagaatgct tcgctagttc
      421 tttcaccatt tcatgtgaat ttgcacggat catgaccgcg tccaagtaac ctgacaatac
      481 attcccggta tcatataccg attccccacg tccgatttga aggtcattgg aattcatgta
      541 cattgcatgg ccgccaagct gtaccattcc gacctcaaaa gaaatcctcg tgcgcgtaga
      601 atgcttttca aaaatcatgc caagtgtctt cccctgcagt atggggggga tttttcctgc
      661 gcgtgcatct tttttcatca ccaaggcttt ttgcaataaa taatcgattt cctcactcgt
      721 atagtctact agcgacaaca aatcacggcc tttaagctga ccttcaattt tttttgcagt
      781 ttttaatgtg gatatatgca ttttcaccat aacgatcatc tccatcctga aatgtggttt
      841 gtatgcttta ttatatgtat gcatatttat aaagtcaaat gaattaatat gaatacatag
      901 aaaaacttta gtcagtcaga agtatgcttt aattatggat ttctattcat ttatagggaa
      961 tttaccaata tgcatttata cacaacttta tgtataaaaa aagaccgtac cttttcaggc
     1021 acgatctttc caatcattat tgaaaaatga tgttatcgac agtttcgcga tccagcttct
     1081 tgatcacttc aacaatcaat ttcactgcgt tttcataatc atcccggtgc aacatagccg
     1141 catgggaatg gatatagcgt gtagcaatac aaatcgccaa ggatggtacg ccgtttccac
     1201 ttacatggat ggagcctgcg tctgttccac cgccaggaat cgcttcgaat tggtatggga
     1261 tgccgtgttc ttccgctgta tcgacgacta ggtcacgcaa tcctttatgg gagaccatcg
     1321 atgcatcgta aagcaggatt tgcggtccgt cacccatttt gctcgtcgac tccttagaag
     1381 ttacgccagg cgtatcccct gcaacaccta cgtcaacagc aaagccgata tccggcttca
     1441 cttttgccgc agctgttttc gcaccgcgca agccgatttc ctcttgaaca gcaccgacac
     1501 cgaacacaat gttcgggtga ttttcatctt tcaacgcttt catgacatcg attgcgattg
     1561 cgcagccgat acggttatcc catgctttcg caagcaagta tttgtcattg ttcatgactg
     1621 tgaattcgaa atacggaacg atcatgtcgc cgggcaaaac gccccactcc attgcttctt
     1681 ccttcgaagt cgcaccgata tcgatgaaca tgtctttaat atccaccggt tttttacgcg
     1741 cttccgcaga aaggatatgc ggaggcttag aaccgatgat gcctgtcact gtatcccctt
     1801 tgcgcgtaac gattgtcaca cgttgggcaa gcatgacttg agaccaccat ccgcctacag
     1861 gctggaagga gatgaatcct ttatcgtcaa ttttcgtcac catgaaaccg acttcatcca
     1921 agtggcctgc taccatcact tttggcccgt ttgcatcgcc gctctttttg gcaattaaag
     1981 aaccgaggcc atcgtattca atttcatcag caaacggctc aatatatttc ttcatgactt
     2041 cacgcggttc acgctcgttt ccaggaatc
//
LOCUS       DASULI010000463         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1260431,
            whole genome shotgun sequence.
ACCESSION   DASULI010000463 DASULI010000000
VERSION     DASULI010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260431"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..196)
                     /locus_tag="VFX34_10005"
     CDS             complement(80..196)
                     /locus_tag="VFX34_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ family sporulation protein"
                     /protein_id="HEX5565286.1"
                     /translation="MSNEVERGVGYGNGFTLIVVLFILLIIVGASYLGGEYC"
     gene            complement(315..1721)
                     /locus_tag="VFX34_10010"
     CDS             complement(315..1721)
                     /locus_tag="VFX34_10010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX5565287.1"
                     /translation="MGDTLAVKPFETYAQEYVGKHLIPGAIIGIDSNGERMYELEFGY
                     RNVAQKLPVSANTVFGIASMTKSFTSAGIMKLQELGKLSVHDKVVDYLPELKKSGGPV
                     ERITLHHLMTHTAGYPPLATHVYARKNSIEKDPSAKDYGLDLLNNPGPHIETYDEMLA
                     FMANDDFEWLGEPGQFYSYSNDSYGLLGVIISRVSGQPYEEFIEENILEPAGMKNSFF
                     DMERLDEAEDVATLYMKAKDGSHVYEAPLWWDAPSMRAVGYLKSTANDILRYLEMYWN
                     EGIVGGTRILSKESIGQMIHPHIEYEPGRCYGYGLRIIPDYFGSTLISHGGGLKGVSS
                     YMCAIIEKKKAGVILTNVSDVPAGDLLMSTLNVTEGRKLDASPSTFETREIDAAELQK
                     YVGSFISSEGMNVKVGLKEGKLTIESNEVCRTLKCIGENFFIDEEENDGILQFIESES
                     GEIERIVYGGRHIMKENV"
     gene            complement(1898..>2645)
                     /locus_tag="VFX34_10015"
     CDS             complement(1898..>2645)
                     /locus_tag="VFX34_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5565288.1"
                     /translation="WSVAVEDVNDGVSFSLNGNESFYAASIIKVPIMAAVFAAAEEGS
                     IRLGDRLPLRRDDIVGGSGVLQFMSPGIELPIHDLLTLMIIQSDNTATNMLIDLIGMD
                     KIQAVMGELGMEQSEIHHKLMIVQANRTASNEITASDVNALLTRIARGQCVSLHASGQ
                     MVRMMKQQQLSYGLIDNLPMINSPIVGTIPDWEFAGKTGNVDGIMHECALLYVCGRCV
                     AVTVLSKGCPEDEARTLIAQIGNDVYSYAR"
BASE COUNT          640 a          789 c          514 g          702 t
ORIGIN      
        1 gaagcgtccc aactgcgcgc ttctctacta tttactcttt tatgcatttt ccgtaaattt
       61 atttacgtaa aaatctaact taacaatatt ctccgcctaa atacgatgcg cctacaataa
      121 tcaaaagaat aaacagaacg acaatcagcg taaaaccgtt cccataccct acaccgcgct
      181 cgacttcatt tgacattccc aacacctcca aaagacttga tgctgttatt ctatgtaatc
      241 aatacgtccc tccccatgac attcccctag gaaataaaaa aggccttcca cgtcatcgtg
      301 aaagacctgc cgttttaaac gttttccttc ataatatgcc tgccgccata cacaatccgc
      361 tcaatttctc cggattcgct ttcaatgaac tgcaggatgc cgtcattttc ctcttcgtca
      421 ataaagaagt tttccccgat gcatttcaat gtcctgcata cttcattcga ttcgattgtg
      481 agcttgcctt ccttcagccc cactttcaca ttcatccctt cgcttgagat gaaggagcca
      541 acgtatttct gaagttccgc cgcatcgatt tcccgggttt caaacgttga aggagaagca
      601 tccaacttcc gtccttccgt gacattcaac gtgctcatca acaggtcgcc tgcaggcaca
      661 tcggatacat ttgtcaaaat cacgcccgcc ttcttctttt cgatgattgc acacatataa
      721 gacgaaacgc ctttcaatcc gcccccatgg ctgatcaacg tggagccgaa ataatccgga
      781 atgatccgca gtccatagcc gtagcatctg cccggctcgt attcgatatg cgggtggatc
      841 atctgcccga tgctttcctt cgataaaatc cgtgtcccac cgacgatgcc ttcattccaa
      901 tacatctcca aataacggag aatgtcattg gccgtcgact tcaaatagcc gaccgcgcgc
      961 atcgacggcg catcccacca aagcggcgct tcatacacat gcgagccatc tttcgctttc
     1021 atgtaaaggg tcgctacgtc ttcggcttca tccaggcgtt ccatgtcgaa aaagctgttt
     1081 ttcattcccg caggttccaa tatgttttcc tcgatgaatt cctcataagg ctgtccgctc
     1141 acccggctga tgatgacccc gagcagtcca tacgaatcgt tcgaatagct gtaaaactgc
     1201 ccaggttcgc caagccactc gaagtcgtcg ttcgccatga acgcaagcat ttcgtcatac
     1261 gtctcgatat gcggtcccgg attgttcaat aggtcgagcc cgtaatcttt agcggaaggg
     1321 tccttctcaa tgctgttctt ccgcgcatat acatgcgtcg caagtggcgg atagcctgcc
     1381 gtatgcgtca tcagatgatg cagcgtaatc cgctcgactg ggccgcccga cttcttaagc
     1441 tccggcaagt agtcgacgac tttgtcatgc acagacaact tcccaagctc ttgaagcttc
     1501 atgatgcctg cgctcgtgaa cgatttcgtc atcgaagcga ttccaaaaac cgtatttgcc
     1561 gacaccggca gcttttgcgc cacattccga tagccgaatt ccagctcata catcctctcc
     1621 ccatttgaat caatgccgat gatcgccccc ggaatcaagt gcttcccgac atattcttgt
     1681 gcatacgtct caaacggctt aactgccaat gtatctccca tgtgaaatcc cccctgtttg
     1741 cgtgttccta ctaactggtt attcgccata tcgcggaatt tccctctttt tatgcgtcat
     1801 caccgacttg gtgggggtta tcaccgactc gcacacgttt atcaccgact ctcacgggtt
     1861 taccaccgac tcgaaaaaac cggatgagga atcctcctca tctggcatac gaatacacat
     1921 catttccgat ttgcgctatc aacgtccgcg cctcatcttc cggacatccc ttcgacagca
     1981 cagtgaccgc cacgcagcgg ccgcacacat aaagcaacgc acactcatgc atgattccat
     2041 cgacattgcc cgtcttcccg gcaaactccc aatccggaat cgtaccgacg ataggggaat
     2101 ttatcattgg caagttatct atcaaaccat atgaaagctg ctgttgcttc atcatgcgga
     2161 ccatctgccc ggacgcgtgc agtgaaacac attgtcccct cgcaatccgt gtcaataaag
     2221 cgttcacgtc agatgccgtg atttcattgc tagccgtccg gtttgcttgg acaatcatca
     2281 atttatggtg gatttcactt tgttccatcc cgagctcacc catgacagct tggattttat
     2341 ccatcccgat taggtcaatc aacatattgg tcgcggtatt atcgctttgg atgatcatca
     2401 atgtcagcaa gtcatggatc ggcagttcaa tgcccggtga catgaattgg agcactccgc
     2461 ttccgccgac gatatcatcc cttcgtaacg gcaaacggtc ccctaaccgg atgctccctt
     2521 cttccgccgc tgcaaacacc gccgccatga tcggtacttt gatgatgctt gcagcgtaaa
     2581 aggattcatt cccattcaac gaaaacgaga caccgtcatt tacgtcctcc actgcaactg
     2641 accat
//
LOCUS       DASULI010000464         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1513905,
            whole genome shotgun sequence.
ACCESSION   DASULI010000464 DASULI010000000
VERSION     DASULI010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513905"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFX34_10020"
     CDS             complement(<1..1230)
                     /locus_tag="VFX34_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5565289.1"
                     /translation="MFHEHGWILKRAALSAERPALIDVHSETCWTYADLKNRIMRWAS
                     FFRAQGYEKGERIAVYSPNSPELFAILFACELQGLVYVPLNWRLSRFELQGLLDDCTP
                     TLLLFHDDFEDRMNGLRAKNAVSLSVIETIVAPSNWQPDVQGNRSDTWMIIYTGGTTG
                     LPKGVMLSYDAVNWNALNTITSWGLTAHDTTINYMPLFHTGGLNALCIPLLMAGGTVV
                     VGHQFEPTEALIATDKYEATISLFVPTMYQLMIGTPYFGQSTFPSMDVFLSGGAPCPQ
                     TVYHAFREKGLRFKEGYGLTEAGPNNFFITPEDAAGKIGSVGKSMLFNEICVLDQDGN
                     PCAAGEIGELYVKGPHMFTGYWNKPSETGEAIVDGWLRTGDLAKVDRDGDAYIVGRKK
                     DMIITGGENVYPQEVEQC"
     gene            complement(1248..1988)
                     /gene="fabG"
                     /locus_tag="VFX34_10025"
     CDS             complement(1248..1988)
                     /gene="fabG"
                     /locus_tag="VFX34_10025"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX5565290.1"
                     /translation="MRLSGKVAIITGGANGIGLAACERFAEEGATVIMADFDEKAGKE
                     QEAVLKEKGHAITFVQVDVVDRESVGRLVEQTLEQHGQIDVLINNAGITKDATLLKMD
                     PADFQRVLDVNLTGVFNCTQEVAPHMIEAGGGKIINTSSVSGVYGNFGQTNYAATKAA
                     VVGMTKTWAKELGRKGINVNAVAPGFTATAMVAKMPENVIKQMASVVSLQRLGKPEDI
                     ANAYLFLASSESDYVHGHVLHVDGGIMM"
BASE COUNT          461 a          643 c          518 g          533 t
ORIGIN      
        1 gcattgttcc acttcttgcg gatacacatt ttcgccgccc gtaatgatca tatccttctt
       61 ccggccaacg atataggcat ccccgtcacg atcgactttt gccagatccc ctgtacggag
      121 ccacccgtcc acaatcgctt cgcctgtctc ggaaggttta ttccaatagc ccgtgaacat
      181 atgcggacct ttgacataca actcgccgat ttcccctgca gcacacggat tgccatcttg
      241 atcaagtacg cagatttcat tgaacagcat gcttttcccg accgaaccga tctttcccgc
      301 cgcatcttcg ggcgtgataa agaaattatt cggccccgct tccgtcaaac catacccctc
      361 cttgaatcgt agcccttttt cgcggaaggc gtggtaaacc gtctgcggac aaggggctcc
      421 gccggacagg aaaacgtcca tcgaaggaaa agtggactgc ccgaaatacg gcgtcccgat
      481 catcagctgg tacatcgtag ggacgaacag cgaaattgtt gcttcgtatt tatccgtcgc
      541 gatgagcgct tccgtcggtt cgaattgatg cccgacgacg actgttccgc ctgccatgag
      601 cagcgggatg cataaggcgt tcaacccgcc tgtatggaaa agcggcatgt aattgatcgt
      661 cgtatcgtgt gctgtcagcc cccagctcgt gatcgtgttg agcgcgttcc aattgaccgc
      721 gtcatacgaa agcatgaccc ccttcggcaa accggtcgta ccgcccgtat agatgatcat
      781 ccacgtatcg gagcgattgc cctgaacatc cggctgccag ttcgacggag caacgatcgt
      841 ttcgatgacg gaaagcgaaa ctgcattttt tgctcttaat ccattcatcc gatcttcgaa
      901 atcatcatgg aagaggagca gggtcggcgt acaatcatcg agcaggccct gcaattcaaa
      961 tcggcttaag cgccagttga gcgggacata gacgagccct tgcagttcgc aggcgaacag
     1021 gatggcgaag agttccgggc tgttcgggga gtagacggcg atgcgctctc ccttttcata
     1081 gccttgcgca cggaagaaag acgcccagcg catgatccga tttttcaagt ccgcgtaagt
     1141 ccagcacgtt tccgaatgga cgtcgatcaa ggcgggccgt tccgcggaga gtgcggccct
     1201 tttcaatatc cagccatgct catggaacac gcggacccct ccttccctta catcattatt
     1261 ccgccgtcaa catgcaggac atgtccgtgg acatagtccg attccgagct tgccaggaac
     1321 agataggcat tcgcgatatc ttccggcttc ccgagccgtt gcaatgaaac gacggaagcc
     1381 atctgcttga tgacgttttc cggcatcttg gcaaccatcg ccgttgccgt aaaacctggc
     1441 gcgaccgcat tgacattgat ccctttccgt ccgagttcct tcgcccacgt cttggtcatc
     1501 ccgacgaccg ccgcttttgt cgccgcgtaa ttcgtctgtc cgaaattccc atagacgccg
     1561 ctgacggaag atgtgttgat aatcttcccg ccgccggctt cgatcatatg aggcgcgacc
     1621 tcttgcgtgc agttgaagac gcccgtcaag ttcacgtcaa gcacccgctg gaaatccgcg
     1681 ggatccattt ttaggagcgt ggcgtctttc gtgattcctg cgttgttgat taacacatca
     1741 atttgtccat gctgttcaag cgtttgctcc actaaacggc cgacgctttc acggtcgacg
     1801 acatcgactt gaacgaacgt gatggcatgc cctttctcct taagcaccgc ttcctgctct
     1861 tttccagcct tctcgtcgaa atccgccatg atgacggtag ctccctcttc agcaaatcgc
     1921 tcacatgcag cgagtccgat tccgttcgcg cctcctgtga tgatggccac ttttcccgat
     1981 agtctcatgc tgtcctctcc ttacgccaaa aagtcttcga tcgtcttcgt cagtacttcc
     2041 aggttatcca ccaatggtga atgccccata tcttcgagtg aaacgaatgt cgcattgtca
     2101 ccgaggtctg ccatgatttc ttccgtcatt tgtgcagtga caacgtaatc ccgat
//
LOCUS       DASULI010000465         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1086817,
            whole genome shotgun sequence.
ACCESSION   DASULI010000465 DASULI010000000
VERSION     DASULI010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086817"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..517
                     /locus_tag="VFX34_10030"
     CDS             116..517
                     /locus_tag="VFX34_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEX5565291.1"
                     /translation="MKQGMEVGRTETIEIIVTEDMLAAFEGEVVHPVYSTVAMTYHME
                     WVSRKIILPFLETHEEGMGASVQLDHLAPSLLDSKVTLTAVLVELRKNIVVTEVTAHN
                     HAGIIGKGIVKQIILPKETIAQKLRNASFSS"
     gene            566..1711
                     /locus_tag="VFX34_10035"
     CDS             566..1711
                     /locus_tag="VFX34_10035"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5565292.1"
                     /translation="MKIEEERVSVASNFDLFEKMAEHEQVVFCNDPSTGLRAIIAIHN
                     TTLGPALGGCRMHPYESVDAALEDALRLSKGMSYKCAAADVDFGGGKAVIIGDPKTDK
                     TPELFRAFGQFVETLGGRFYTGTDMGTTMEDFVHSMKETNCIVGTPEAYGGGGDSSIP
                     TAEGVLYGIKATNLELFGNDELGGHSYAVQGLGKVGFKVAVGLLEAGAHVYVTDIDEE
                     SLHAIEEKAATTPGTVKVVASGDIYTQEADIFVPCALGGIINDQTINQFKVKAVAGSA
                     NNQLLHENFGKKLQETGILYAPDYIINSGGLIHVADELYGFNHERVLAKTKHIYDAIL
                     EVYRLAKTSGICTMEAANAMCEKKMDSRGGRNSFYTPSIKPKWAIRK"
     gene            1733..2788
                     /gene="pdhA"
                     /locus_tag="VFX34_10040"
     CDS             1733..2788
                     /gene="pdhA"
                     /locus_tag="VFX34_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5565293.1"
                     /translation="MEIDFPIKKVIDDEGILLDSSYEEQINEQLVRDFYYHMVRIRTF
                     DRKAINLQRQGRLGTYAPFEGQEASQVGSSLALGADDWIFPTYRDHGATLTYGADMAR
                     TYLYWNGRVEGCVPPEGKRIFPPAVPIATQLPHAVGAAWAEKRKGTRNAAIAYFGDGA
                     TSEGDFHEALNFASVFQVPVVFFNQNNGYAISVPVEKQMNSATIAQKSVAYGMPGIRI
                     DGNDCFAVYFETKKAFDDARNGKGPTLIEAVTWRTGAHTTADDPSKYRPVEQGKHIID
                     PLVRLERFMRNYGYWDEEWVAGIKDKTADEIEAAVDEMERFPAPNVEDLFDHVYAELP
                     ADLAAQKEAYLAQLGGK"
     gene            2790..>3534
                     /locus_tag="VFX34_10045"
     CDS             2790..>3534
                     /locus_tag="VFX34_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017378730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5565294.1"
                     /translation="MAIDLKKSAEPTETTKLMTMVQAINSGMETMLEEDERTIIMGED
                     IGKNGGVFRATEGLVEKFGEDRVVDTPLSEAGIIGTAIGLAINGFRPVAEIQFLGFIY
                     PAYEQIMTHVSRIRMRTMGTFTVPMVIRAPYGAGIRAPEIHSDSSESLFTHMPGLKVV
                     CPSGPYDAKGLLIAAMEDPDPVLVLEPMRSYRAQREDVPLGKYTIEIGKGKIVCEGTD
                     VTIIAWGAMVAVAEKAAEQAREMGISCEIL"
BASE COUNT         1011 a          707 c          983 g          833 t
ORIGIN      
        1 aagtgactct ccgagaaaaa atgtaagcgc tctcatttgt ttttgaagtc ctgatcagat
       61 ttgcaccgac aatcgtagtt tatgggcgag aatcatagta tatagggggc aggcgatgaa
      121 gcagggaatg gaagtcggca ggacagaaac gatagaaata atcgtgactg aagatatgtt
      181 ggctgcattt gagggtgaag ttgttcaccc cgtctattcg acagtggcga tgacctatca
      241 catggaatgg gtttcccgga aaatcattct gccttttttg gaaacacatg aagaagggat
      301 gggggcgtcc gttcagctag atcatctagc accttctcta ctagattcaa aagtgacact
      361 gacagcagta ttagttgaat tacggaaaaa tattgtcgtt acggaagtta ctgcgcacaa
      421 ccatgcagga atcatcggta aaggtattgt caaacaaatc attcttccaa aagaaacgat
      481 tgctcaaaaa ctgaggaatg cttcgttttc ttcctaggaa gagaagttta gcaggttgat
      541 agtaaatgct cagggggcaa gaactatgaa aattgaagaa gaaagagtgt cagtcgcaag
      601 caactttgat ctttttgaaa aaatggcgga acatgaacaa gtcgttttct gcaatgatcc
      661 atcgacaggc ctacgcgcaa ttatcgctat ccataatact acacttgggc ctgcgctagg
      721 cggctgccgc atgcatccat acgaaagcgt ggacgcggct ctggaagatg cacttcgttt
      781 atcgaaaggg atgagctata aatgcgcagc tgctgacgtt gattttggcg gcggcaaggc
      841 ggtcatcatc ggagatccga aaacggataa gacacccgaa ttgttccgtg cattcggcca
      901 attcgtagaa acgcttggcg gccggtttta caccggcacc gatatgggga cgaccatgga
      961 ggatttcgtc cactcaatga aagagacaaa ttgcattgta ggtacacccg aagcgtatgg
     1021 tggcggaggg gactcttcca tcccgacagc agaaggtgtt ttgtatggta ttaaagcgac
     1081 aaatctggag cttttcggaa atgatgaatt aggagggcat tcctacgctg tccaaggtct
     1141 agggaaagtg ggattcaaag ttgctgttgg gttacttgaa gcaggtgcac acgtttatgt
     1201 gacggatatt gatgaagaga gtctgcatgc catcgaagaa aaggcggcta caacgcctgg
     1261 aaccgtgaaa gtcgttgcaa gcggggatat ttatacacag gaagcggata tttttgttcc
     1321 ttgtgcatta ggcggaatca tcaacgacca gacaatcaat cagttcaaag tgaaagcggt
     1381 cgcaggatcg gccaacaatc agctgcttca tgaaaatttc ggaaaaaagc tgcaagagac
     1441 aggaattctt tatgcgccgg attatattat caactccggc ggtctcatcc acgtggcaga
     1501 tgaattgtac ggtttcaatc atgagcgggt attggcgaag acaaaacata tttatgatgc
     1561 gattttagaa gtatacaggt tggcaaaaac gagcggcatc tgcacgatgg aagccgcaaa
     1621 tgcgatgtgt gagaaaaaaa tggacagtag aggcgggcgg aacagctttt acaccccatc
     1681 cattaaaccg aagtgggcaa tccgcaaata atcgtgagtg ggggtcatgt agttggaaat
     1741 tgactttccg attaagaaag taatcgatga tgaaggaatc ttgctggatt cttcctacga
     1801 ggaacagatt aatgagcaac tggtcagaga tttctattac cacatggtac ggatccggac
     1861 attcgaccgg aaagcgatca acttgcagcg acaaggacgt ctagggacat atgctccgtt
     1921 cgaagggcag gaagcttcgc aagttggcag ttcgcttgct ttaggcgcgg atgactggat
     1981 ttttccgacc tatcgtgacc acggtgcgac gttgacgtac ggtgcagata tggcgaggac
     2041 ctatttatac tggaatggcc gggttgaagg ttgcgtccca ccggaaggga aaaggatttt
     2101 tccgccggca gtcccgattg cgacacagct gccacatgcg gtaggagcgg cttgggcgga
     2161 gaagaggaaa gggaccagaa atgcagccat cgcttatttc ggcgacggtg ccacttcaga
     2221 aggcgatttt catgaagcgt taaattttgc aagtgtgttc caggtgcccg tcgtcttttt
     2281 taatcaaaat aatggctatg cgatctctgt gccggtggaa aagcagatga attctgcaac
     2341 aattgcacaa aaatcagtcg catacgggat gccgggaatc cgtatcgacg gcaatgattg
     2401 ttttgcagtc tattttgaaa caaaaaaggc atttgacgat gcccgaaacg ggaagggccc
     2461 gacgttgata gaagcggtca cttggcggac aggtgctcat acgactgcgg atgatccttc
     2521 caaatatcgg ccagtggaac aaggcaagca tattatcgac ccgcttgtcc ggttggaaag
     2581 attcatgcgg aattatggct attgggatga agaatgggtc gcgggaataa aagacaaaac
     2641 ggctgatgaa atcgaagcgg cagtggatga aatggaacgc ttcccagctc cgaacgtcga
     2701 agatcttttt gatcatgtct atgcagagtt gccagcagac ttggctgcac aaaaagaggc
     2761 ttaccttgca caattagggg ggaagtgaca tggctattga tttgaaaaaa tcggccgaac
     2821 ctacggagac gactaaactg atgacaatgg tccaagcgat caatagcggg atggaaacaa
     2881 tgcttgaaga agatgaacga acaattatca tgggcgagga catcggtaaa aatggcggtg
     2941 tattccgggc gaccgagggg cttgtagaga aatttgggga agatcgtgta gtggatacgc
     3001 cgctttcaga agccggcatt atcggaacgg ccatcggtct agccataaat ggtttcagac
     3061 ccgttgcgga aattcagttt cttggtttca tttatccggc atacgagcaa atcatgacgc
     3121 atgtttcccg gatccgaatg cggacgatgg ggacttttac ggtaccgatg gtcatccgtg
     3181 caccgtatgg agcgggaatc cgagcccctg aaatccactc agacagttcg gaatccttgt
     3241 tcacacatat gccgggcctc aaggtcgtct gtccatctgg accttatgat gcaaagggtt
     3301 tattgatagc tgcaatggaa gatcctgatc cggtgcttgt gctggaaccg atgaggagtt
     3361 accgcgctca aagggaagat gttccgctag gcaaatatac aattgaaatc ggcaagggga
     3421 aaatcgtgtg tgaaggaacc gatgtgacaa tcattgcatg gggagcaatg gtggcagtgg
     3481 cggaaaaggc agcagagcag gcaagggaaa tgggcatcag ttgtgaaata cttg
//
LOCUS       DASULI010000466         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1397558,
            whole genome shotgun sequence.
ACCESSION   DASULI010000466 DASULI010000000
VERSION     DASULI010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397558"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..683
                     /gene="mraZ"
                     /locus_tag="VFX34_10050"
     CDS             252..683
                     /gene="mraZ"
                     /locus_tag="VFX34_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016992543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5565295.1"
                     /translation="MFMGEYQHTVDAKGRLIVPSKFREYLMDGFVLTRGLDNCLFGYP
                     MEEWKRLEEKLKALPVTKKDARAFARFFFSGATEVEIDKQGRVNIPSSLLDYAKVEKD
                     CVVIGVSSRIEIWSKSSWDSYYDESEQSFNEIAENIIDFDF"
     gene            722..1654
                     /gene="rsmH"
                     /locus_tag="VFX34_10055"
     CDS             722..1654
                     /gene="rsmH"
                     /locus_tag="VFX34_10055"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5565296.1"
                     /translation="MFDHTTVLLHEAVDGLNIKSDGIYVDCTLGGAGHSLEIVKKLSA
                     GGRLICFDQDTTAIEAAKVKLNDHLEQVTFIHSNFRNLKEELAKIDVHTVDGILYDLG
                     VSSPQLDTPERGFSYNHDAMLDMRMNTEAALTAYDVVNEWPYEDLVRIFFRYGEEKFS
                     KGVARKIEEARKNAPVRTTGELVELIKEGIPAAARRTGGHPAKRVFQAIRIAVNDELG
                     AAEDSLTDALTVMNPGGRICVITFHSLEDRLCKTIFKEASSMPDLPPNLPIVPEGMEP
                     DFKLVTRKPILPSSEEIEVNKRSRSAKLRIVEKK"
     gene            1671..2051
                     /gene="ftsL"
                     /locus_tag="VFX34_10060"
     CDS             1671..2051
                     /gene="ftsL"
                     /locus_tag="VFX34_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008498018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX5565297.1"
                     /translation="MALEQRSFQMNTIHEVHAPQSPKTNETQQPARRSRKLFSKGEKF
                     LFVMFAAVLVIFSTMILHTQAQINDTNKEVQKINNEIVEISKQNTELSIQVSEKSTYE
                     VIWNKAKELGLNLNEKNVKVVPGR"
     gene            2087..>2673
                     /locus_tag="VFX34_10065"
     CDS             2087..>2673
                     /locus_tag="VFX34_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEX5565298.1"
                     /translation="MFIIFGGLFFLLFGRLLSIQITGQAEGRQMAAMAEAKYAREAIL
                     KAERGKIVDRNGELIASDTLSYRLIAILSEKATEKGAKKPRHVTDFEKAADLLSRFIP
                     MEKEQMIHIRDSAIKRNADTYQIEFGKAGRDISHETMLAIKEAAENEGVTGLQFIEDK
                     KRFYPNGNFASYLIGFAMREEDENKKVSTVGKMGL"
BASE COUNT          825 a          497 c          674 g          677 t
ORIGIN      
        1 atggcaccca ccaagtcatc tatttataaa taataaatta tgccgatgca ggcctcaaat
       61 actgcgtcgg cgtttttttg tgcatttatg agttatttag cgtatttttg catgttttga
      121 aactttgctc gattcatagc gatatctcaa tgaaacaagt actatataca cattttacta
      181 ttaaaataga atatgagtgg aggaatgtgg agggatgtgg tacattaaaa acatatagtg
      241 gggtgaagag tatgttcatg ggcgaatatc agcacactgt agatgcaaaa ggccgtttga
      301 tagtcccttc gaaattcagg gaatatctga tggatggttt tgtgctgact cgtggattgg
      361 ataactgtct cttcggttac cctatggagg aatggaaaag gcttgaggaa aaattgaagg
      421 cgctgccggt tacgaagaaa gatgcccgag catttgcccg attcttcttc tcaggtgcca
      481 cggaagttga aattgacaag cagggccgtg tcaatattcc atcctcatta ttggattacg
      541 cgaaagttga aaaagattgt gttgtcatcg gtgtttcgag ccgcattgaa atttggtcga
      601 aatcatcctg ggattcctat tacgacgagt ccgagcaatc attcaacgaa atcgcggaaa
      661 atatcattga ttttgacttc taattttatg acatttactt attggaaagt aggcggtttc
      721 gatgttcgat cacacaactg tattacttca cgaagccgtc gatggcctca acattaaatc
      781 ggatggcatt tatgtcgatt gcacgcttgg cggtgccggg catagcttgg aaattgtgaa
      841 gaagctttcg gcaggcggca gattgatttg cttcgatcag gatacgacgg caattgaagc
      901 tgccaaagtg aagttgaacg accatttgga gcaagttaca ttcatccact cgaacttcag
      961 gaatttgaaa gaagaactgg cgaagattga tgtacatact gtggatggca tcctttatga
     1021 cctaggcgtt tcctccccgc agcttgatac gccggaacgc ggattcagct acaatcatga
     1081 cgcaatgctc gatatgcgca tgaacacgga ggcggcgttg acggcatatg acgtggtgaa
     1141 tgaatggcca tatgaagatt tggtacgcat ctttttcaga tatggcgaag agaagttttc
     1201 caaaggagtt gcccggaaga ttgaagaagc gcggaaaaac gctcccgtca ggacgacagg
     1261 cgaactcgtg gaattgatca aagaaggcat acccgcagcg gcacgaagaa cgggcgggca
     1321 tccagcgaaa agggtgttcc aggcaatccg gatagctgtg aacgatgaac tgggagcggc
     1381 agaggattca ttgactgatg ccttgacagt catgaatcct ggcggccgca tttgcgtcat
     1441 cactttccac tcacttgagg acagattatg caagacaatc ttcaaagagg cgtcttccat
     1501 gcctgatctg cctccgaatc ttcccatcgt gccagaaggg atggaacccg atttcaaatt
     1561 ggtgacgagg aaaccgattc ttcctagcag tgaggaaatt gaagtgaata agagatcacg
     1621 atctgcaaag ctgcggatcg tagagaaaaa ataaaggggg gacatgttaa atggcattag
     1681 aacagagaag ctttcaaatg aatacgatac atgaagtaca tgcaccgcaa tcacctaaga
     1741 caaatgaaac acaacagcct gcgaggcgtt caagaaaact tttctcaaaa ggggagaaat
     1801 tcctgttcgt gatgttcgcg gcagttctcg taattttctc tacaatgatt ttgcatacgc
     1861 aggcccaaat taatgatact aacaaggaag tgcaaaaaat caacaatgaa atcgtggaga
     1921 tttcaaaaca gaatacagaa ttgtccatac aagtgagcga aaaatctaca tatgaagtaa
     1981 tatggaataa agcgaaggag ctcggcttga atcttaacga gaaaaacgta aaggtcgtgc
     2041 ctggacgatg aaaaagaagt ttcgatttca atggggagcc ttcctgatgt ttataatttt
     2101 cggagggctc ttttttctat tgtttggacg attgctaagc attcaaataa caggacaggc
     2161 agaaggaagg caaatggctg ccatggctga agcgaaatat gcgagagagg ccatcttgaa
     2221 agctgagagg ggaaaaatcg tcgatcgcaa tggcgagttg atcgcttctg atacattgag
     2281 ttatcgattg attgctattt taagtgaaaa agcaactgaa aaaggggcga aaaagccgcg
     2341 gcatgtaaca gattttgaga aagccgccga tttgctgagc aggttcatcc ctatggagaa
     2401 agagcaaatg attcatatca gggattcggc aattaaaagg aatgcggaca cctatcagat
     2461 cgagttcgga aaagcgggac gcgatattag ccatgaaacg atgcttgcca ttaaagaggc
     2521 agcagaaaat gaaggagtta caggtcttca attcatcgaa gataagaagc gtttttatcc
     2581 aaacgggaat ttcgcctcgt atttaatcgg atttgcgatg cgtgaagaag atgagaataa
     2641 aaaagtttcc actgtcggta aaatgggcct gga
//
LOCUS       DASULI010000467         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_813611, whole
            genome shotgun sequence.
ACCESSION   DASULI010000467 DASULI010000000
VERSION     DASULI010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813611"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFX34_10070"
     CDS             complement(<1..876)
                     /locus_tag="VFX34_10070"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5565299.1"
                     /translation="MKKENLNVAIVGATGAVGTKILEKLLERKFPANSIKLLASKRSA
                     GLEITAGGQTYIVEETVPESFEGVDIAFFSAGGSISQKFAHEAVKRGTVVIDNTSAFR
                     MDENIPLVVPEVNPQTLAQHSGIIANPNCSTIQMVAALQPIKEKFGINRIIVSTYQAV
                     SGAGAEAIDELEQQSTQFAERSHIKAELLPSASAERHYPIAFNAIPQIDLFDESGYTL
                     EELKMMNETKKIFGDQEMAVTATCVRLPVVTGHSESVYIETGDENATVADIRAAMENA
                     PGVVVQDDPSAQLYPM"
     gene            complement(1051..1635)
                     /locus_tag="VFX34_10075"
     CDS             complement(1051..1635)
                     /locus_tag="VFX34_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008178956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HEX5565300.1"
                     /translation="MLQGKRIGLGITASHCTYDQILPVISDLQENGATVVPVITHSVL
                     TAATRFGTGEEWIARIEAATGEKVISTIVGAEPFGPSSPVDCMIIAPMTGNSISKFAN
                     AATDSPVLMAAKATLRNGSPVLIGISTNDALGLNGPNIMKLLNMKNVYFIPFGQDDPF
                     KKPNSLISDFTLMVPAAAAALEHRQLQPLLIIHK"
     gene            complement(1648..>2215)
                     /locus_tag="VFX34_10080"
     CDS             complement(1648..>2215)
                     /locus_tag="VFX34_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565301.1"
                     /translation="DSSTILYPGVATKEWLAPFIEAGIPIRPYLKEMEFVWENAVLTA
                     EGFLLEYYPSAKRRISGEHFHIAGFGRVGKTTAAVLSSLGASVTILARSADQLGEAAA
                     IGYQTMLLPTDTVDLQGVLVNTIPAKWLSVAEDSTLRIYDLASAPGCLRDYAPDEYYT
                     IHLGLPGKHFPVDAAKALSEALLRMNSR"
BASE COUNT          544 a          527 c          500 g          644 t
ORIGIN      
        1 catcggatac aattgtgcgg atggatcgtc ctggacgaca actcccggtg cgttttccat
       61 cgcagcacgg atgtcggcaa cagttgcatt ttcatcgccc gtttcgatat agacggattc
      121 cgaatggccc gtcacgacag gaagacggac gcaagtggca gtaacagcca tttcttggtc
      181 gccgaaaatc ttttttgtct cattcatcat tttcaattcc tctaaagtgt accccgattc
      241 atcgaaaaga tcgatttgcg ggattgcgtt gaacgcaatc ggataatggc gttctgcaga
      301 tgcgcttgga agcagctccg ctttgatatg cgatcgttct gcaaattgcg tgctttgctg
      361 ttcgagttca tcgattgctt ctgcacccgc tcctgaaacc gcttgatagg ttgatacgat
      421 gatccgattg atgccgaatt tttccttgat cggctgaagc gcggcaacca tttggattgt
      481 tgaacagttc gggttcgcaa tgattcccga atgttgtgcc aaagtttgcg gattcacttc
      541 cggaacgaca agtggaatgt tttcgtccat cctgaacgca ctcgtgttat caataacgac
      601 agtgccgcgc tttaccgctt cgtgcgcaaa cttctgggag atggagcctc ccgcgctgaa
      661 gaatgcgatg tctacgcctt caaatgattc aggcaccgtt tcttcgacaa tatatgtttg
      721 accgcctgca gtgatttcaa gtcctgcaga acgtttcgaa gccagcaatt ttattgaatt
      781 cgcagggaat tttctttcca aaagtttttc aaggattttt gttcccactg ctcctgtcgc
      841 tccaacgatt gctacattca gattttcttt tttcatctca atctcccttt cattagtaag
      901 gacttcggat atatatacga gtttttgttt attgatgcaa ctgaatgaaa ttcaaattta
      961 tgcaagaaca aaacttcacc ctatgtccct tatgtgttca ttggagaaag tataacatat
     1021 tcgaaagttt tctgcgttgt atactttatt ttatttatga ataattaata gtggttgtaa
     1081 ttgtcggtgt tcgagggcag cagcagcagc aggaaccatc aatgtaaaat cggagatcaa
     1141 tgagttcggt tttttgaaag gatcgtcttg accgaacggg atgaaatata cattcttcat
     1201 gttcaataat ttcatgatgt tcggtccatt caatcctaat gcgtcatttg tcgaaatgcc
     1261 gatcagcact gggcttccgt tccgcaatgt agcctttgcc gccatcaata ccggtgaatc
     1321 ggtcgctgca ttggcgaatt tgcttatgga atttccagtc atcggcgcaa tgatcatgca
     1381 atcgaccggg gaacttggac cgaacggctc agcccctaca atcgtggaga tgactttttc
     1441 acctgtcgca gcttcgattc tcgcgatcca ttcctcacct gtcccgaacc gggtcgctgc
     1501 ggttaggacg gaatgtgtaa taactggaac tacagtcgcc ccgttttcct gcaaatcgct
     1561 tatgacgggc agaatttggt catacgtaca gtgggatgcc gtaataccaa ggccaattct
     1621 tttcccttgt agcatctttt tcccctttca tctactattc atcctcagca acgcttcgga
     1681 caaagcctta gcagcatcta ccggaaaatg ttttcccggc aatccgaggt gtattgtata
     1741 gtattcatca ggggcatagt cccttaggca accgggtgca gatgccaaat cataaatgcg
     1801 caatgtggaa tcctctgcga cactcagcca tttcgcaggt atcgtattta caagaacccc
     1861 ctgtaagtca actgtatcgg tcggcagcag cattgtttga taaccgattg cggcagcttc
     1921 gccgagctga tctgccgaac gtgccaatat tgtaacagag gcaccaagtg acgaaaggac
     1981 agcagctgtc gttttgccga ctcttccgaa gccggcaatg tggaaatgct cacctgaaat
     2041 tcgacgtttt gccgaagggt agtattccag aagaaatccc tccgctgtca aaactgcatt
     2101 ttcccagaca aactccattt ccttcaagta gggacgtatg gggatgccgg cttcaatgaa
     2161 aggcgcaagc cattcttttg tagctacacc aggataaaga atagttgatg aatcg
//
LOCUS       DASULI010000468         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_832574, whole
            genome shotgun sequence.
ACCESSION   DASULI010000468 DASULI010000000
VERSION     DASULI010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832574"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFX34_10085"
     CDS             complement(<1..382)
                     /locus_tag="VFX34_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003736619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX5565302.1"
                     /translation="MKNDQQLLLGSHVSMSGKSMLLGSSEEALGYGANTFMIYTGAPQ
                     NTRRKPIEELNIEAGLLHMKENGLSNIVVHAPYIINIGNTTKPETFRLGVNFLQSEIQ
                     RTAALGADQIVLHPGAHVGAGADKG"
     gene            complement(372..1673)
                     /locus_tag="VFX34_10090"
     CDS             complement(372..1673)
                     /locus_tag="VFX34_10090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5565303.1"
                     /translation="MSKFTDYQFKPFLREAIARLGFENPTPIQKEMIPLILKGSNAIG
                     QAHTGTGKSHSFLIPILERLDEGKEELQAVITAPTRELALQLYEELNKMTENTMIRSS
                     LLIGGTDKQRSIDKLKSNPHIIVGTPGRIRDLAEEGALAIHTATILVIDEADLAFDMG
                     FIEDIDKFASKMPTDLEMYVFSATIPEKLKPFLAKYMENPVHVQIGNKKPLTEGMKYS
                     LVPVRSMDRKKKLLHILHAINPYLAIVFTNTKQNADEVADYLAEQGIKAGRIHGDLSP
                     RERTRMMKQIRDLEYQYIVATDLAARGIDIPGVSHVVNYELPDDLEFFIHRVGRTARA
                     GLEGIAITMYEPSEDDKIVQIEKMGIPFIHEDVKKGEWVEVKERHARKKRVKETDELD
                     RKAASFVRKPSKVKPGYKKKMAKDIEKFKKRERRLKRRNEK"
     gene            1918..2220
                     /gene="vrrA"
                     /locus_tag="VFX34_10095"
     CDS             1918..2220
                     /gene="vrrA"
                     /locus_tag="VFX34_10095"
                     /inference="COORDINATES: protein motif:HMM:NF025544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VrrA/YqfQ family protein"
                     /protein_id="HEX5565304.1"
                     /translation="MGNPYGSTMQGPFQNTSKMEMYMQTANRFLNTAQQYAPLVQQIA
                     PMFQNLPAMWRLYKGFQSLPSAGSTAGAAASRAAAAGESGPLAGASNPRIFQPPKL"
     gene            2285..3265
                     /locus_tag="VFX34_10100"
     CDS             2285..3265
                     /locus_tag="VFX34_10100"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5565305.1"
                     /translation="MKVKKISPRGYCYGVVDAMVRARNAALDKTLPRPIYILGMIVHN
                     KHVTDAFEEDGIITLDGKNRLEILEKVETGTVIFTAHGVSPEVRELARRKGLVSIDAT
                     CPDVTVTHDLIAEKTAEGYDIIYIGKKYHPEPEGAIGVAPHAVHLIETLDDVDGLELT
                     NEKLLVTNQTTMSQWDVAHIMDALKEKFPHIEVHKEICLATQVRQEAVADQAGEADLL
                     IVVGDPKSNNSNRLTQVSVEIANTPSYRISDVSEINIDWLQGVETVAVTAGASTPTLI
                     VREVIGFLENFDPEDPATHVPERKFKLERILPKIKNPEPVTRIEPYSVTE"
     gene            complement(3321..>4154)
                     /locus_tag="VFX34_10105"
     CDS             complement(3321..>4154)
                     /locus_tag="VFX34_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016993530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX5565306.1"
                     /translation="ISVYAAHTNLDVAPGGVNDLLASRLGLENIEVMQPTISDPLYKL
                     AVFCPVEHADGLREVLAKSGAGAIGDYAGCSFTSTGTGRFTPSAGADPFIGEVGKMEE
                     VTEERIEVVLPGPIRSKVLKAMLAAHPYEEPAYDFFVLDQRTEEFGLGRVGKLPEPME
                     LHKFAQFVKNALDVPALRIVGDSEKMVHKIAVLGGSGSKYIRAAKRKGADVFVTGDMD
                     FHSAQEAELMDLAIVDPGHHVEKVMIEGVAGHMEQACKEKGFDVEFIESQIDTEPFKF
                     I"
BASE COUNT         1034 a         1060 c          938 g         1122 t
ORIGIN      
        1 tgcctttatc cgcgccagcc ccgacatggg ctcccggatg caagacgatt tggtctgcgc
       61 caagagctgc agttctttgg atctccgatt gcagaaagtt gacgcctaac cggaatgttt
      121 ccggcttagt cgtattccca atattgatga tgtaaggtgc atgtacgacg atgttcgaaa
      181 gaccgttctc tttcatatgc aacagaccag cctcaatatt cagttcctcg ataggctttc
      241 tccgcgtatt ttgaggcgcc cctgtataaa tcatgaatgt attggctccg tagcctagcg
      301 cctcttcact tgaacctaga agcatgctct tgccgctcat cgaaacatgt gaacctaata
      361 gcagttgttg atcatttttc atttcttcgt ttcagtctcc tttccctctt cttgaatttc
      421 tctatatcct tggccatttt ctttttataa ccaggcttca cctttgatgg ttttcgaaca
      481 aaagatgcag ctttgcgatc gagttcatcc gtctctttaa cccgtttctt ccgggcatga
      541 cgctccttga cttctaccca ttcgcctttt ttgacgtctt catggatgaa tgggatgccc
      601 atcttttcaa tctgcacgat tttgtcatcc tctgaaggct cgtacattgt gattgcaatc
      661 ccttcaagac ctgcccgggc cgtcctgccc acccggtgga tgaagaactc caagtcatcc
      721 ggcaattcgt agttcacgac atggctcact ccagggatgt cgataccacg agccgcaaga
      781 tcagttgcaa cgatatattg atactcaagg tccctgatct gtttcatcat ccgggtccgt
      841 tcacgaggag acaggtcgcc gtggatcctg cctgctttta tgccctgctc ggcaaggtaa
      901 tcggcgactt catccgcatt ttgcttcgta tttgtaaata cgatggccaa ataaggatta
      961 attgcatgca aaatatgcaa aagctttttc ttgcggtcca tgctccggac aggaacaagc
     1021 gaatatttca tcccttccgt caacggcttt ttatttccaa tttggacatg gacgggattt
     1081 tccatatact tggccaggaa aggcttcagt ttttcgggaa ttgttgctga aaagacgtac
     1141 atttccaagt ccgtcggcat tttggacgca aacttgtcga tatcctcaat gaatcccatg
     1201 tcaaaagcca agtctgcttc atcaatcacc aaaatggttg ccgtatggat tgccaatgct
     1261 ccttcttcag caaggtcacg tatccttcct ggcgtcccga caatgatatg gggattggat
     1321 ttcagtttgt cgatggagcg ttgcttatcc gttccaccaa tcaaaagtga acttctgatc
     1381 attgtatttt ccgtcatttt attcaattct tcatatagct gcaaagcaag ttccctcgta
     1441 ggagcggtga tgaccgcctg cagctcttcc ttcccttcgt ccagacgttc aaggatcgga
     1501 ataaggaagc tatgcgactt gcctgttcct gtatgtgctt gtccgatcgc attggatcct
     1561 ttaaggatta acgggatcat ttctttttgt ataggtgttg ggttttcaaa ccccaatcgg
     1621 gcaattgctt cccgcaggaa aggtttgaat tgataatcag tgaatttgga cattcatatg
     1681 cctcctaacg tattcctatt gtagcatgat tccgttccgc agtatgtgat tttcgcatag
     1741 tatagattgt gaaagttcac aatgaccaaa tgaaaggggt tatccatgag gtacgaatcc
     1801 ttctatccat tcgcgcgcac ccaaaactcg ccgtttgcga tgaggcagcc attccgcagc
     1861 ccacagcaac agccgaactt ttcccaaccg ccgagacaac ctttccgggg ggctccgatg
     1921 ggaaatccgt acggatcaac gatgcaaggt ccatttcaaa atacatccaa aatggaaatg
     1981 tacatgcaaa cagccaaccg gtttttaaat acagcacaac agtacgcgcc acttgtacag
     2041 caaatcgcac cgatgtttca gaacttgcct gcgatgtgga gattgtataa aggctttcag
     2101 tctctacctt ccgctggaag tacagcaggg gcggccgctt cccgcgccgc agcggctggg
     2161 gaatcaggcc cactagccgg cgcttccaat ccaaggatct ttcaacctcc caaattataa
     2221 ttttgaaacg tgtgcctgac tccgctataa tggatacaga tccctttaga ggagtgtgga
     2281 gtgaatgaaa gttaaaaaga tttccccgcg cggctattgt tacggggttg tcgatgcaat
     2341 ggtcagagcc agaaatgcag cgctcgacaa aactttgccg agacctattt atatattagg
     2401 catgattgtt cataataaac acgttacaga cgcatttgag gaagatggga tcatcacctt
     2461 ggatgggaag aaccggttgg agatcctgga gaaagtggaa actggcaccg tcattttcac
     2521 cgcacacggc gtgtcgccgg aagtccgtga gttggcccgc cgcaaaggcc ttgtttcaat
     2581 tgatgcgact tgcccggatg taacagtcac ccatgacctt attgcagaaa agactgcgga
     2641 aggttacgac atcatctata tcggcaagaa gtatcatccc gagccggagg gggcaattgg
     2701 cgtagcgcct catgcagttc atctgatcga gacgttggat gacgtcgatg ggctcgaact
     2761 tactaatgaa aagcttcttg tcacgaatca gacgacgatg agccagtggg acgttgcaca
     2821 tatcatggac gcgttaaaag agaaattccc tcatattgaa gtgcacaagg aaatctgcct
     2881 tgcgactcaa gtgagacaag aggcagtcgc ggatcaagcc ggagaagcag atctgttgat
     2941 cgttgtagga gatccgaaaa gcaataactc caaccgtttg acgcaagtat cggtggaaat
     3001 tgcgaatacg ccttcttacc ggatttcaga tgtttccgag attaatatcg actggctgca
     3061 aggtgttgaa acggtggcgg ttacagccgg cgcatccacg ccgacgctga ttgtccgtga
     3121 agtgatcggc ttcttggaaa acttcgaccc tgaagatccg gcgactcatg tgcctgagcg
     3181 taaattcaaa ttggagcgga tcttgccgaa aatcaagaat ccagagccag taacacgaat
     3241 cgaaccatat tcagttacag aataaatgaa agaggctgtt ccgacgtcgt tcaacgacat
     3301 ggaacagccc ttttttcatt tcatataaat ttgaacggct cagtatcaat ctgtgattcg
     3361 atgaattcga catcaaagcc tttttcttta catgcctgtt ccatatgccc tgcaacccct
     3421 tcaatcatca ctttttccac atgatgaccc ggatcgacaa ttgcaagatc catcagttct
     3481 gcttcctggg cactgtggaa gtccatatcg ccggttacga atacatctgc ccctttccgt
     3541 ttcgcagccc taatatactt actgccactg cctccaagca ctgcaatttt atggaccatt
     3601 ttctcggaat cgccaacgat cctgagagcc ggaacgtcca gcgcattttt gacgaactgg
     3661 gcaaacttgt gtagctccat tggctcgggt aatttaccta ccctgccgag accgaactct
     3721 tccgtccgtt gatcaagcac aaaaaaatca tacgcgggct cttcatatgg atgggcagcc
     3781 aacatcgctt tcagcacttt cgatcgaatc ggacccggaa gtacgacttc gattcgttct
     3841 tcagttacct cttccatttt tccgacttcc ccgataaacg ggtcagcccc tgctgacggc
     3901 gtgaagcgcc ctgttccggt ggaagtgaag ctgcatccgg cataatcccc gatagcacct
     3961 gcgcctgatt tggccaacac ttcccgtaac ccatcggcat gctcaacagg acaaaagacg
     4021 gccaatttat aaagcggatc ggaaatcgtc ggctgcatca cttcgatatt ttcaagaccc
     4081 aggcgggatg caagcagatc attcacccct ccaggtgcga cgtccagatt cgtgtgtgca
     4141 gcatagaccg aaat
//
LOCUS       DASULI010000469         5422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_910655, whole
            genome shotgun sequence.
ACCESSION   DASULI010000469 DASULI010000000
VERSION     DASULI010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910655"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /gene="carB"
                     /locus_tag="VFX34_10110"
     CDS             <1..1700
                     /gene="carB"
                     /locus_tag="VFX34_10110"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5565307.1"
                     /translation="WNTDETSVYEWRKDFGLAPIYKKVDTCSAEFDSETPYFYGTYEE
                     ENESIKSNKKSVIVLGSGPIRIGQGVEFDYATVHSVWAIKEAGYEAIIINNNPETVST
                     DFSISDKLYFEPLTIEDVMHIIDVEQPEGVIVQFGGQTAINLAAALEARGVKLLGTTL
                     EDLDRAENRNKFEKALHEIGIPQPLGKTAVSVEEAVEIATEIGYPVLVRPSYVLGGRA
                     MEIVYEENDLLYYMEHAVEASPEHPVLIDRYLTGTEIEVDAISDGERVLIPGIMEHIE
                     RAGVHSGDSIAVYPPQNLTQEQIATIADYTTRLAKGLNIVGLLNIQYVISEGNVYVIE
                     VNPRSSRTVPFLSKITNIPMANIATKAILGQSIEEQGYSDGLASTIKGVYVKVPVFSF
                     AKLRRVDITLGPEMKSTGEVMGKDTTLEKALYKGLVAAGMEIKEYGSVLMTVSDKDKE
                     EAVNIARRFKEIGYHILATEGTASTIEAAGIPVRTVGKIGADGPTLLDVIRSGKAQFV
                     INTLTRGKQPERDGFRIRRESVENGVPCLTSLDTAEAVLRVIESMTFQAEEMKKQEV"
     gene            1703..2479
                     /locus_tag="VFX34_10115"
     CDS             1703..2479
                     /locus_tag="VFX34_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012293188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX5565308.1"
                     /translation="MILRDSMQVVSNLEIAERIYEMTVSGKLTNEISSPGQFVHIRVV
                     DSFEPLLRRPISISSINKERNEFTMIYRVDGRGTAVLSDKRAGDQVDVLGPLGNGFPV
                     EETRPGETAILVGGGIGVPPLYELSKQLSAKGVTCIHVLGFQTASAVFYEEEFAELGK
                     THVVTVDGTRGVTGFVTDVMKSLPTEFQTYYSCGPMPMLNAVQNMYEGKKGYLSFEQR
                     MGCGIGACFACVCKTTSERGTDYVKVCSDGPVFPAGVVQL"
     gene            2476..3390
                     /locus_tag="VFX34_10120"
     CDS             2476..3390
                     /locus_tag="VFX34_10120"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX5565309.1"
                     /translation="MNRLQVELPGLSLKNPIMPASGCFGFGKEFGNLYDLSVLGSIMI
                     KATTLEPRFGNPTPRVAETSSGMLNAIGLQNPGLEGVLQNELPWLGQFDLPIIANVAG
                     SETEDYVEVAKQISKAPNVHALELNISCPNVKCGGIAFGTDPSSAKELTQAVKAVSEV
                     PVYVKLSPNVADIVQIAKAVEEGGADGLTLINTLIGMRLDGRTGKPVIANGTGGLSGP
                     AVKPVAIRMVYEVYRNTELPIIGMGGVTGVDDVIDFLSAGASAVAVGTANFVNPFVCP
                     EIIEALPSKLDQLGVQSVTELIGRSHRS"
     gene            3387..4100
                     /gene="pyrF"
                     /locus_tag="VFX34_10125"
     CDS             3387..4100
                     /gene="pyrF"
                     /locus_tag="VFX34_10125"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5565310.1"
                     /translation="MKRTPMIALDFPNEGKTLEFLSKFDESLAVKIGMQLYYSEGPSI
                     LDKIKEAGHDIFLDLKLHDIPNTVMSSMEVLAKLGVSLTNVHAAGGREMMEAALEGLD
                     KGTAVGMKRPALIAVTQLTSTSEQQVRVEQLINATLEDSVLHYAKLTKQAGLDGVVCS
                     VHEAKAIEEVCGKEFLKVTPGIRMADNDAGDQKRIATPFEAKQAGSTHIVVGRAITGA
                     ADPVEAYRKVLAQWEGAAQ"
     gene            4097..4732
                     /gene="pyrE"
                     /locus_tag="VFX34_10130"
     CDS             4097..4732
                     /gene="pyrE"
                     /locus_tag="VFX34_10130"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX5565311.1"
                     /translation="MSREKDVAQILLKIGAVEFNPNDPFTWASGIKSPIYCDNRLTMS
                     DPYGRKLIAEGLAALIKEQFPDTTLIAGTATAGIPHAAWVADILELPMLYIRSKAKGH
                     GRSKQIEGKVKPGDKAVIIEDLISTGGSSLTAAEALLDENINVQGVVSIFTYELEAAD
                     HAFGEKGLSYHSLTNFGALIEAAQESGGINQDAIPGLIDWHTKLKAGLLAK"
     gene            complement(4785..>5422)
                     /locus_tag="VFX34_10135"
     CDS             complement(4785..>5422)
                     /locus_tag="VFX34_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEX5565312.1"
                     /translation="GTVKIPLDPRKNAIENAQKFYSRYTKSKNALVQIAEQLQKTDED
                     IQYFEMIKQQVMQASPEDIEEIREELAELGYLRARKGKKKKKPTKPQPESYESSSGIP
                     ISVGKNNKQNDYLTFKIAARQHTWLHTKDIPGSHVVIHHENPDEKTLEEAAILAAYFS
                     KARESSSVPVDYTEIRHVKKPNGAKPGFVIYFEQKTLFVTPDEDIVRKLRK"
BASE COUNT         1546 a         1135 c         1388 g         1353 t
ORIGIN      
        1 tttggaacac agacgaaacg agtgtgtacg agtggagaaa agatttcggt ctcgcgccaa
       61 tctataaaaa ggtagacacg tgctctgccg agtttgattc ggaaacacca tacttctacg
      121 gaacatacga agaagagaat gaatccatta aatcaaacaa gaagagtgtc atcgtgcttg
      181 gttccggacc gatccgaatc gggcaaggag tagaatttga ctatgcaacg gttcactccg
      241 tctgggccat caaagaagca ggctacgaag cgattattat caacaacaac cctgaaacgg
      301 tttcaacgga tttctccatc tccgacaaac tatactttga accgctcaca atcgaagatg
      361 tcatgcacat cattgacgtg gagcagccgg aaggcgtcat cgttcaattc ggagggcaaa
      421 cagcgatcaa cctcgccgca gcacttgaag cacgaggtgt aaaactgctc ggcacaacac
      481 ttgaagatct agaccgggca gaaaaccgaa acaagtttga aaaggcttta catgaaatcg
      541 gtatcccgca accgcttggc aaaactgcag tatcagttga ggaagcagtg gagattgcaa
      601 cagaaatcgg gtatccggtt cttgtccgtc cttcctacgt gctcggtggc cgtgcgatgg
      661 aaatcgtgta cgaggaaaat gatcttctct actacatgga gcatgcggta gaggcgagtc
      721 cagagcatcc agtcttaatt gaccgttatt taacgggtac tgaaattgaa gtggatgcaa
      781 tcagtgacgg ggaaagggta ttgattccag gaatcatgga gcacatcgag cgtgctgggg
      841 ttcattccgg ggattcaatt gcggtttatc cgccacagaa cctaacacag gaacaaatcg
      901 ccacaatcgc tgactatacg acacgtcttg caaaagggtt gaatattgtc ggtttactaa
      961 acatccagta tgtcatttcg gaaggaaatg tgtatgtcat cgaagtgaat ccccggtcaa
     1021 gccgaacagt tccgttcctt tcgaaaatta ccaacatccc gatggcgaac attgcaacga
     1081 aggcgattct aggccaatcc atcgaagaac aaggctattc agacggactc gcttcgacaa
     1141 taaaaggggt ctatgtgaag gttccggtct tcagctttgc aaaactccgt agagttgaca
     1201 taacgcttgg accagaaatg aaatcgacag gcgaggttat ggggaaagat accacgcttg
     1261 aaaaggcttt gtataaagga ttagttgccg cagggatgga gattaaggaa tacgggtcag
     1321 ttctaatgac cgtatcggat aaagataaag aagaagcggt caacattgca cggcgcttta
     1381 aggaaatcgg ctaccacatt ctagcgacag aaggaacggc tagcacgatt gaagctgctg
     1441 ggatcccggt ccgaacagtc ggcaaaatcg gagcagacgg accaacatta ttagacgtca
     1501 tccgaagtgg taaagcacag ttcgtcatca acacgttgac gagaggaaaa cagccggagc
     1561 gtgacggttt ccgcatccgc cgtgagtcag ttgaaaatgg cgtaccttgc ctaacgtcac
     1621 tcgatacagc tgaagcggtt cttcgtgtaa tcgaatcgat gaccttccaa gcggaagaga
     1681 tgaaaaagca ggaggtataa gcgtgatttt acgagactcc atgcaagtcg tgtccaatct
     1741 agaaattgca gagcgcattt atgaaatgac tgtttcagga aagctgacga acgagatcag
     1801 ctctcctgga cagttcgtcc acatccgtgt tgtggacagc ttcgagccgt tgcttagacg
     1861 tccgattagt atttcatcga tcaataaaga aagaaatgaa tttacgatga tctaccgtgt
     1921 agatggtaga gggacagcgg ttttatccga caagagagca ggagatcaag tcgatgttct
     1981 tggtccactc gggaacggat ttccggtcga agaaacacga cctggtgaga cagctattct
     2041 agttggtggc ggcatcgggg ttcctccttt atatgagttg tccaaacaac tgtctgcaaa
     2101 aggggtcaca tgcattcatg tcctcggttt tcaaaccgcc tctgcggtat tttatgagga
     2161 agagttcgcg gaacttggca agacacatgt agtaacggtg gacggaacaa gaggggttac
     2221 tggattcgta acggatgtca tgaagtcact accgaccgaa tttcaaacgt attacagctg
     2281 tggtccgatg ccgatgttga atgccgtcca gaacatgtac gaagggaaga aaggatacct
     2341 ttcattcgag cagcgaatgg gctgcggaat cggagcttgc tttgcatgcg tatgtaaaac
     2401 gacaagtgag cgcggtacag attatgtgaa agtttgctca gatggtcccg tatttccagc
     2461 aggggtggtg caactatgaa tagattacaa gtagaacttc caggactttc attaaaaaat
     2521 ccaatcatgc cggcatctgg ctgcttcgga ttcgggaaag agtttggaaa cctatacgat
     2581 ttatctgtac ttggatcgat catgatcaaa gcgacaacat tggagccacg cttcggaaac
     2641 ccgacacccc gtgttgcgga aacttcttca ggtatgttaa atgcaattgg gttgcaaaat
     2701 ccaggtttag aaggtgtctt acaaaatgaa cttccgtggc ttgggcaatt tgatcttccc
     2761 atcatcgcga acgttgcggg gagtgaaacg gaagattatg tggaagtggc aaagcagata
     2821 tcaaaagcac caaacgtcca tgcactcgag ttaaatattt cctgtccaaa tgttaaatgt
     2881 gggggtattg ctttcgggac agacccgtca agcgcaaaag aactgacaca agcagtcaaa
     2941 gcagtttcgg aagtgcctgt ctatgtgaag ctttcaccga atgttgctga catcgtgcaa
     3001 attgcaaaag cagttgaaga aggcggagcg gatggactga cacttattaa cacattgatc
     3061 gggatgcggc ttgatggacg aacagggaag ccggttatag ctaacggaac aggcgggtta
     3121 tccggccctg cggtgaagcc tgttgccata cggatggtat atgaagtgta ccgaaacacg
     3181 gaattgccga ttatcggaat gggaggcgta acgggtgtgg atgatgtcat tgatttccta
     3241 tcggcaggtg cgagtgctgt cgcggttgga actgcgaatt tcgttaatcc gttcgtctgt
     3301 ccagaaatca ttgaagctct tcctagtaaa ctagaccagc ttggggtgca atctgttacg
     3361 gaattgattg gaaggagcca tcgttcatga aacgtacacc gatgatcgca ttagactttc
     3421 cgaatgaagg aaagacgctc gagtttctgt ctaagtttga tgaatctctt gccgttaaaa
     3481 tcgggatgca gctttattac agcgaaggtc catctattct cgataaaata aaagaggctg
     3541 gacatgatat ctttctcgat ttgaaactcc atgacattcc gaacaccgtc atgtcctcga
     3601 tggaagtgct agcgaaactt ggtgtttcgt taacgaacgt acacgcagcc ggtggacgcg
     3661 agatgatgga agcggcactc gaaggattgg ataaggggac agccgttgga atgaagcgtc
     3721 cagccttgat tgctgttaca cagttaacat caactagtga gcagcaggtt cgggtggaac
     3781 agctcatcaa cgccacatta gaggattctg tccttcatta tgcgaagctt acaaagcaag
     3841 caggactcga tggtgttgtt tgttcagtcc atgaggcgaa agcgattgaa gaagtatgtg
     3901 gcaaggaatt cttaaaggta actccgggta tccggatggc ggataacgat gctggagatc
     3961 aaaaacggat tgccactcct tttgaagcta aacaagcagg ctcaacacat attgtagttg
     4021 ggcgtgcaat cactggagcg gcagatccag tggaagcgta cagaaaagta cttgcacaat
     4081 gggaaggagc agcacaatga gtagagaaaa agatgttgcg caaattttat tgaagatcgg
     4141 agcagtcgaa ttcaatccaa atgatccgtt tacatgggct tcaggaatca aatcacctat
     4201 ctattgtgat aatcgcttaa caatgtcgga tccgtatggg cgaaagctga ttgcagaagg
     4261 attggctgcg ctcattaaag agcaatttcc cgatacaacg ctcattgcag gaacagcaac
     4321 cgcaggtatt ccgcatgctg catgggtagc tgacattctt gaattgccga tgctgtacat
     4381 ccggtcaaaa gccaagggcc atggacggtc caagcagatt gaagggaaag tgaaaccggg
     4441 agacaaggcg gtcattatcg aggatttaat ctctacaggc ggatcaagcc tgactgcagc
     4501 tgaagctcta ctcgatgaga acatcaacgt ccaaggcgtt gtttcgattt ttacatacga
     4561 actagaggcg gcagaccatg cttttgggga aaaaggattg agctatcaca gtttgacaaa
     4621 ctttggtgca ttgattgaag cagctcagga gagcgggggg atcaatcaag acgcgattcc
     4681 tggcttaatt gactggcata cgaaactgaa ggcaggtttg ctcgcaaaat aaaaagtgaa
     4741 aaaaggtggc gaattcgttt tttaacgaag ctcgccacct cattttattt cctcagcttc
     4801 cgtacaatat cctcatcagg cgttacaaac aatgtcttct gctcaaaata aataacgaat
     4861 ccaggtttgg caccattcgg ttttttcaca tggcgaattt ctgtgtaatc aactggcaca
     4921 gatgaagatt cgcgggcctt actaaagtat gcagccaata ttgccgcttc ttcaagcgtt
     4981 ttttcatccg gattttcatg atggatgacg acgtgggatc ccggaatatc ctttgtatgg
     5041 agccatgtat gctgacgggc tgcaatttta aatgtcaaat aatcattctg tttattattc
     5101 ttgccgacag agattggaat gccagaagaa gattcatatg attccggttg cggttttgtc
     5161 ggttttttct ttttcttgcc ttttcgagca cgcagatacc caagctcggc taattcttcc
     5221 cggatttcct caatgtcttc cggagacgct tgcatcactt gctgcttgat catttcaaaa
     5281 tactgaatat cctcgtcggt tttttgaagt tgctccgcaa tttgaacaag ggcattcttc
     5341 gacttcgtgt agcgggagta aaacttttgt gcattttcaa ttgcattttt acgaggatct
     5401 agagggattt ttactgttcc tc
//
LOCUS       DASULI010000470         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_833067, whole
            genome shotgun sequence.
ACCESSION   DASULI010000470 DASULI010000000
VERSION     DASULI010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833067"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFX34_10140"
     CDS             <1..795
                     /locus_tag="VFX34_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX5565313.1"
                     /translation="AIRSMTMEERMTICNMSIEAGAKAGLISPDETTIEYLRGRRHVP
                     EGEAFEEATEKWLSLATDEGAAYDTVLEINADEIEPFVTWGTNPSMGSGISAHVPSAE
                     DFENESDKEALKKALAYMGLEEGTPLTDIEIQHVFIGSCTNARLSDLRAAAEVIAGQK
                     VHPNVTAIVVPGSRTVKRQAEEEGLDKIFIESGFEWRESGCSMCLAMNDDVVPAGERC
                     ASTSNRNFEGRQGAGSRTHLMSPAMAAAAAISGRFTDVREMKLAHV"
     gene            939..1523
                     /gene="leuD"
                     /locus_tag="VFX34_10145"
     CDS             939..1523
                     /gene="leuD"
                     /locus_tag="VFX34_10145"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5565314.1"
                     /translation="MKPINEVRSVMTPLNRKNVDTDQIISKEFLKRIERTGFGKYLFH
                     HWRFHADGTENKEFVLNDDRFKDSKILVAHDNFGCGSSREHAPWAILDYGFEVVIAPS
                     FADIFHNNCFKNGILPIRLRVEEVEELLSKGESAPYALEINLAEQTVTGEDGKTYTFD
                     IDPYYKQMLLNGWDEIALTFQYEEHIAEYEKQHA"
     gene            complement(1611..2285)
                     /locus_tag="VFX34_10150"
     CDS             complement(1611..2285)
                     /locus_tag="VFX34_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009765905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX5565315.1"
                     /translation="MNRMENTEIRDEQLQQFACLLQDWIPKDARLAIALNGCYYCCST
                     GEDEFERLDGESVLAGSIASKVLMRREKTGVMMDPTLSGTPAYGVGYPIEINGEEAAL
                     VVILPPDHGSAKSDPLRLLTGKLDDEWYPVPIEQITHIESFQKKTFFYTEDGQFSISN
                     TLKELDKRLPEIFLRIHRSYIVNTARILRISRDFSSNLVVTLTDGTELPVSQSYVNDV
                     KCVLGF"
BASE COUNT          680 a          609 c          662 g          601 t
ORIGIN      
        1 gcaatccgtt ccatgacgat ggaagagcgg atgacgattt gcaatatgtc catcgaagcg
       61 ggggcgaaag cagggttgat cagccccgat gagacgacga ttgagtactt acgaggtcga
      121 cgtcatgtcc ctgaaggtga agctttcgaa gaggctacgg aaaaatggct ctcattggca
      181 acggatgaag gggcggcata cgataccgtc cttgaaatca atgcagatga aatcgagccg
      241 ttcgtcacgt ggggcacgaa tccttcgatg gggtcaggca tcagcgctca cgttccatcg
      301 gctgaggatt tcgagaatga atccgataag gaagctctga aaaaagcgct cgcttatatg
      361 gggcttgaag aagggacgcc gcttacggat atcgaaatcc agcacgtctt catcggctcc
      421 tgcacgaacg cccgcttgag cgatctgcgg gcagctgcag aagtgatcgc agggcaaaaa
      481 gtgcatccga atgtgacggc aatcgtcgtg ccaggttctc ggacggtgaa acgacaggcg
      541 gaagaggaag gcttggataa aatcttcatt gaatcaggat tcgaatggcg tgaatcgggt
      601 tgcagcatgt gccttgcgat gaacgatgat gtcgtaccgg ctggcgaacg atgcgcatcg
      661 acatcgaacc ggaacttcga aggcagacaa ggggcgggtt cacgcaccca tctgatgagc
      721 cctgcaatgg cggcagcggc agccatttct ggacgtttca ctgatgtgcg tgaaatgaaa
      781 ttggctcacg tttaataaac aaaagcggag ggcgcctgcc tagaggcgac aggcctaaga
      841 cgaagatacg aagtggcgct ctttgccacg cagtggcttt gacttatgac ccgagcttct
      901 ggcgcccgga gctggatgaa ggaagaaagg atgattccat gaaaccgatc aatgaagtgc
      961 gcagcgtcat gacccccttg aatcgaaaga atgttgatac ggaccagatc atatcgaagg
     1021 aattcctcaa gcggattgaa cggaccggct tcgggaaata ccttttccac cattggcgtt
     1081 tccatgcgga cggcacggaa aacaaggaat tcgttttgaa cgacgaccgg ttcaaagact
     1141 cgaaaatcct cgttgcccat gacaatttcg gctgcggctc ctcaagggag catgcaccgt
     1201 gggcgatcct ggactatgga ttcgaagtcg tcatcgcacc atcgttcgcg gatatcttcc
     1261 acaataactg cttcaagaac ggcatcctgc cgatccgtct acgagtagag gaagtcgaag
     1321 agcttttatc caaaggcgaa tccgccccgt atgcattgga gatcaatctt gcagagcaga
     1381 cggtgacggg cgaagacgga aaaacataca cattcgacat cgacccgtat tataagcaga
     1441 tgctattgaa tgggtgggac gaaatcgcgt tgacgttcca atacgaggag catatcgccg
     1501 aatacgaaaa acagcatgct tgatgaatgg gctgcagatg aaatcaacag tgatctcatc
     1561 tgcagtctat tcttttgtcc attccctctc ctaaccctgc cttccttcat tcaaaacccc
     1621 agaacgcatt tcacatcatt cacatacgac tggctcaccg gcagttctgt tccatctgtt
     1681 aacgtcacca cgagattcga agagaaatcg cgtgagatcc tcaaaattct agcggtattc
     1741 acgatgtatg aacgatggat gcgcaaaaag atttcaggaa gccgcttatc caattccttc
     1801 aatgtgttcg aaatgctgaa ttgtccatcc tccgtataga agaatgtctt cttttgaaag
     1861 ctttcgatat gggtgatctg ttcaatcgga accgggtacc attcatcgtc caattttccg
     1921 gtgagcagcc gcagtggatc gcttttggcg gacccatggt cgggcgggag gatgacgaca
     1981 agagcggctt cttccccgtt tatttcgata ggatagccga cgccgtaagc aggagtcccc
     2041 gacagtgtcg gatccatcat gacacccgtt ttttccctcc gcatcaacac ctttgacgcg
     2101 atgcttcctg ctagaaccga ttcgccgtcc aaccgttcaa attcatcttc acccgtggaa
     2161 caacagtaat agcagccgtt caatgcaatc gcaagccggg catcttttgg aatccaatcc
     2221 tgcaacaggc aggcaaattg ctgcaattgt tcgtcgcgta tttccgtgtt ttccattcga
     2281 ttcatcttcc ccttttcgat ttcatcgtgt tatttcctca ttcatcccct gaaactatcc
     2341 ttttgtcgga atgcacagac agttttaata ttctgttata tagtaatgta taacaactta
     2401 ctctgttgtg gaacagtaag aaagtatcaa ttattctatt aaatgaaagg tgtgtatgaa
     2461 atggcaaatg gaaatgaacg attggaacaa gtggagcagt tagagagaag ctggcaagag
     2521 gatgaacggt ggggaggaat cgagcgcggc ta
//
LOCUS       DASULI010000471         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_56867, whole
            genome shotgun sequence.
ACCESSION   DASULI010000471 DASULI010000000
VERSION     DASULI010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56867"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFX34_10155"
     CDS             <1..417
                     /locus_tag="VFX34_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5565316.1"
                     /translation="KREFKRMAHELSLGIEFSAALRTMERRIDHKEFQLFAATMLIQK
                     KAGGNLSAVLDEMSRTLDERKLLKQEVKTMTAEQRYVSTLVPVIPIFLVLMMNNIIDG
                     FLDPLFTGIGLILLTFFLFGTVLTFIIVRKITNIRV"
     gene            422..1354
                     /locus_tag="VFX34_10160"
     CDS             422..1354
                     /locus_tag="VFX34_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5565317.1"
                     /translation="MDGIIALFTFLFVAAIGISLRAFYVYLNEKRKLHEEISESVYIE
                     TWIRKKKSRKEKVLEKLFKFADDFSDLGQRVNFFSENVEVKKLLIQAGHPYGLTVARF
                     QGLKIFMLTAGFFIGGILFILRFPLSQFMLILMPLAGFGGTVLWLRSKARKRQEEISY
                     QLPDFLDTMSVTLQAGVGMDQALRDIVHYFEGPIKEEFGRFIQELSVGVPRAEAYRSL
                     LQRNDSKEFQILIKSLLQGERLGVPIADTFKHQAEEMRKLKKELIKEKAAKASPKVTL
                     VTTFLVLPSALVLIGGLMIINIFNQNKGIFELFN"
     gene            1384..1590
                     /locus_tag="VFX34_10165"
     CDS             1384..1590
                     /locus_tag="VFX34_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003351236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5565318.1"
                     /translation="MKEKMMTAWTKMMYPVKNEKGAQSLEWLGIAALLILVLGIISTA
                     LKGQEGTITELITTIVTKIKGMVG"
     gene            1624..>2945
                     /locus_tag="VFX34_10170"
     CDS             1624..>2945
                     /locus_tag="VFX34_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5565319.1"
                     /translation="MIRKKIVPFILLLMLLLLAACSDKAKENEKVKADETVASDEKAE
                     DKDIDLQEEQANQEDNKSEEVAAAPLPQSLEELEQLPTGYTSFISILDKDGQAQIDEL
                     TSHLPDISGEPTQNELDHYYNELLAVFQQDFNGPGELIARLKFHAIGSPDIDNPRMQF
                     KENMNVLVILDASGSMGKYVGGQTQMEAAKKAIVGFLESLPKEANVGLRVYGHEGTGS
                     SADKAMSCSSSDLLYPLQAYDKASFKQSLDQVKPAGWTPTELAIREAKKDLAEFDGEH
                     NTNIVYLVGDGISTCNDDPVAAAKDLYDSNITPIINVIGFNVDNEGQRQLKEVAKAVE
                     GTYQDVQDAATLQKELDQANEIAKQWADWKKRESQNLGHERLKKDLEIFVFDSQQFTK
                     IVDERQQVGFTLQYLYQTKKIMSSESYDYLVKRNTDYHSWVQTEYEAL"
BASE COUNT          899 a          602 c          744 g          700 t
ORIGIN      
        1 aaaagggagt tcaaacggat ggcccatgag ctgtcattag gcatcgaatt ctctgcggcg
       61 ctcaggacga tggaaagacg gatcgatcat aaggaattcc agttgttcgc ggcgacgatg
      121 ctcatccaga aaaaagcagg gggcaacttg tccgcggtac ttgatgaaat gagccggacg
      181 cttgatgaac ggaagctgtt gaagcaggaa gtgaagacga tgactgcgga acaacggtat
      241 gtctccacac tcgttccggt catcccgatt ttcttggtcc ttatgatgaa caacatcatt
      301 gacggcttcc ttgatccttt atttacagga atcggattga tcttgttgac gttcttcctc
      361 tttgggacgg tcctgacatt tatcatcgtc aggaaaatta cgaatatcag ggtgtgaatg
      421 aatggatggc ataattgcat tatttacctt tttatttgta gcggcgatcg gaatttcgtt
      481 aagggctttc tatgtctact tgaatgaaaa aaggaagctg catgaagaaa tcagcgagtc
      541 cgtctatatc gaaacatgga tccgtaaaaa gaagtcgcgc aaagagaaag tgttggaaaa
      601 gctattcaag ttcgcagacg atttttccga tctcgggcag cgcgtcaact tcttcagtga
      661 aaacgtagag gtgaagaagc tcctcataca agcaggacat ccatacggat tgacggttgc
      721 ccgtttccag gggctgaaaa tcttcatgct tactgcagga tttttcatcg gcgggattct
      781 tttcatcctc agatttccat tatcgcaatt catgctgatt ctcatgccgc ttgcaggatt
      841 cggaggaacc gtcctttggc tcagatccaa agcgaggaaa aggcaggaag agatttccta
      901 ccagcttccc gacttccttg acacgatgag cgtcaccttg caggcaggtg tcgggatgga
      961 tcaggcgctc cgcgacatcg tccattattt cgagggaccc atcaaagagg aattcggccg
     1021 tttcatccag gaattgagcg tcggtgtgcc gcgggctgaa gcgtacagat cattgcttca
     1081 acggaacgac agcaaggagt tccaaatact gatcaaatca ctattgcaag gggaacggct
     1141 gggagttccg attgcagaca cgtttaaaca ccaagccgaa gagatgagga agctaaagaa
     1201 agaattgatc aaggagaagg cagccaaagc atcaccgaaa gtgacgttag taactacttt
     1261 ccttgtattg ccctccgccc tcgtcctgat cggaggcttg atgattataa atatcttcaa
     1321 ccagaacaaa gggattttcg agttattcaa ttaaaataca tttagttaag gagagaattg
     1381 aagatgaaag aaaaaatgat gacagcttgg acgaaaatga tgtacccggt gaagaacgaa
     1441 aaaggggcgc aatcgttgga atggcttgga atcgcggcat tgctcatttt ggtgctagga
     1501 attatttcga ctgcgttgaa aggccaagag ggaacaatca ctgagctgat cactacaatc
     1561 gttacaaaga tcaaagggat ggtaggttaa taaaacaact tgatttcaaa aagaggagta
     1621 cacatgatta gaaaaaaaat agttccattc attctcctgc ttatgcttct tctattggca
     1681 gcatgcagcg ataaggccaa agagaatgaa aaggttaaag cggatgaaac ggttgcatca
     1741 gatgaaaaag cagaggataa agacatcgat cttcaagagg agcaagcaaa tcaagaggat
     1801 aacaagagtg aagaggttgc agctgccccg ctcccgcaat cattagagga gcttgagcag
     1861 ctgcctacgg gatatacctc tttcatctcg attctggata aagatgggca agcccaaatt
     1921 gacgagctga cttcccattt gcctgatata tcaggtgagc cgacgcagaa cgaattggat
     1981 cattactaca acgaactatt ggcggttttc cagcaagact tcaacggacc gggtgagctg
     2041 attgcaagat tgaagtttca tgcaatcggc agcccggata tcgacaatcc ccgaatgcag
     2101 ttcaaggaga atatgaatgt ccttgtcatt ttggatgctt caggcagcat ggggaaatat
     2161 gtcggcggcc aaacgcaaat ggaagctgcg aaaaaggcaa ttgtagggtt tcttgagagc
     2221 ttgccaaagg aggcaaatgt cggacttcgc gtttatggac atgaagggac cggcagttca
     2281 gcagataaag cgatgtcgtg ttcaagcagt gatttgctct atccgcttca ggcatacgac
     2341 aaagcttcat ttaaacagtc tcttgaccaa gtgaagcccg caggttggac gccgacagaa
     2401 cttgccatca gggaagcgaa aaaggacctt gcggaattcg atggagagca taatacgaac
     2461 attgtctacc ttgtgggaga tggcatctcg acttgcaatg acgacccggt tgccgctgca
     2521 aaagaccttt atgattccaa cattacaccg atcatcaatg tcatcggatt taatgtcgat
     2581 aatgaggggc agcgtcaact gaaagaagtg gcgaaggcgg ttgaagggac gtatcaagat
     2641 gtccaagacg cagcaacact tcaaaaagag ctggatcagg ctaatgaaat tgccaagcaa
     2701 tgggccgatt ggaaaaaacg agagtcacaa aatctgggcc atgaaagatt gaaaaaagac
     2761 ctggaaatat ttgttttcga cagtcagcaa ttcacgaaga tcgtagacga aaggcagcaa
     2821 gtcggtttca ctttacaata tctgtatcag acgaaaaaaa tcatgtcgtc tgaaagctat
     2881 gactatctcg taaagcggaa tacagactac catagttggg tgcaaaccga atatgaagca
     2941 ctcaa
//
LOCUS       DASULI010000472         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1586999,
            whole genome shotgun sequence.
ACCESSION   DASULI010000472 DASULI010000000
VERSION     DASULI010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586999"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFX34_10175"
     CDS             complement(<1..366)
                     /locus_tag="VFX34_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014823066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5565320.1"
                     /translation="MPPNGSIFHRRDYPSPNPNIRLEEITYWSDGLKVKGLLAEPVNG
                     GNYEGIVYLRGGIQHVGMVRPARIAQFAAQGFVVFAPYYRGNRGGEGRDEFAGDDRQD
                     AVNAVEVLKQSPKLNKDRIH"
     gene            complement(356..736)
                     /locus_tag="VFX34_10180"
     CDS             complement(356..736)
                     /locus_tag="VFX34_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010303917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5565321.1"
                     /translation="MLIVLKHEGKWLVTRHSIRGIEFPGGKAEDGETIVEAAIRETIE
                     ETGVTIKDLVKFAEYVVISNVKFCKAVFTGKVSSIEEYPQLFETEGALWMTDEELDNC
                     DELSFHMKDAGMAEIRKWVNAYAS"
     gene            complement(843..1205)
                     /locus_tag="VFX34_10185"
     CDS             complement(843..1205)
                     /locus_tag="VFX34_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5565322.1"
                     /translation="MENDKVRMYVSIANQQIYAHKHDSPWEYEVEMSREFLPVFNRLF
                     SQIGQLEFGNLLRGHTRPYFQYHYDRDNHEIDRRTMKLYALIHEFTDEETKNFIEQLP
                     YFTKGRPKITSAPVQPLN"
     gene            complement(1261..2586)
                     /locus_tag="VFX34_10190"
     CDS             complement(1261..2586)
                     /locus_tag="VFX34_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEX5565323.1"
                     /translation="MSHIQFIRNLIIETPSKPGNFAKVASAIGELKGDIGDIQTIKVG
                     TLSTIRDVSIQCTSEEQLESIVAAINAIGDGIHVHAVTDDVLHAHEGGKISMKSRVEV
                     RSLGDLRRIYTPGVANVCKVIQNDPVQADYFTGISNTVAIVTDGTAILGLGNIGPVAG
                     MPVMEGKAVLFDQFAGISGIPILLDTSNPDEVVETVKHIHQSFGGILLEDIGSPHCFE
                     IEERLKAELDIPVMHDDQHGTAVVTLASALSACRQSGVELSESVVGQIGLGAAGLAIS
                     RMFMAYGVKEMYGVDPNPEAADRLEQHGGTALDSVDEIMKTCDIVIATTGVQGLIKPE
                     MVRKGQIILALSNPNAEIEPEAALEAGAAFAADGRQVNNILGFPGIFRGALNAHAKDI
                     TYPMLIAAALAILDSTKPGDLVPHPLDPAVHANVASAVERVANEEENTI"
     gene            complement(2706..2885)
                     /locus_tag="VFX34_10195"
     CDS             complement(2706..2885)
                     /locus_tag="VFX34_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HEX5565324.1"
                     /translation="MNMNSIMASQLRSLQSTVQLSVMNKALSVNANAVTDMLQSMQDQ
                     PAVHPYKGSAVDVKA"
     gene            complement(2948..3397)
                     /locus_tag="VFX34_10200"
     CDS             complement(2948..3397)
                     /locus_tag="VFX34_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEX5565325.1"
                     /translation="MSKELMDELNVQVSTWSVMYAKLHNYHWYVKGKQFFTLHAKFEE
                     LYNEATAHMDEIAERVLTLGGNPTATLKEHLEQSVVKEAKGDEQAEDMVKTIADDFGN
                     IMKSLKKGMEYAAEAGDDMTEDLLNATYQSIEKHQWMLNAFLGEDNK"
BASE COUNT          827 a          933 c          656 g         1005 t
ORIGIN      
        1 gtgaatacgg tccttgttca acttgggact ttgcttcagc acctcaacag cattgaccgc
       61 atcttggcga tcgtcacctg cgaattcatc gcgtccttcg ccacctctgt tgcctcggta
      121 ataaggcgcg aaaacaacaa agccttgtgc agcgaattgg gcaatgcgtg ctggacggac
      181 catgccgaca tgctgaatgc ctccgcgcaa gtagacaatc ccttcataat tgcctccgtt
      241 aaccggttcg gcaagcaatc ccttcacttt caatccatcc gaccaatacg ttatttcctc
      301 aagacggatg tttggattcg gcgatggata atccctccga tgaaaaatag atccgttagg
      361 aggcatatgc attcacccac ttccgtattt ccgccattcc ggcatctttc atatgaaaac
      421 tcaactcgtc acagttgtcc aattcttcgt ccgtcatcca taaagcgcct tccgtttcga
      481 ataactgcgg atattcttcg atgctggaca cttttccggt aaataccgcc ttgcaaaatt
      541 tcacattgct gatgacgacg tattcggcaa acttgacgag atctttaatc gtcaccccgg
      601 tttcttcgat tgtttcccgt atcgctgcct caacaatcgt ttctccatct tcagccttgc
      661 ctcccggaaa ttcgatgccg cggatcgagt ggcgtgtaac tagccatttc ccctcatgtt
      721 ttaagacaat gagcacatgc ctcgcctcca tgcccattcg attttcaccg aatgtcagct
      781 cgacatgtgc gccttgtaaa tccctaaatg tcgttgctat ttgactccac gtcctttccc
      841 aatcagttca gtggttgaac aggggcagat gtaatctttg gcctcccctt cgtaaaatac
      901 ggaagctgtt caatgaagtt cttcgtctcc tcatccgtga actcatggat taatgcatat
      961 agtttcatgg ttcttcgatc tatttcatgg ttatcccggt cgtagtggta ttgaaaatat
     1021 ggcctcgtat gtcctcttag gagattgccg aactccaatt gccctatttg tgaaaataac
     1081 ctattgaata ccggcaggaa ttccctgctc atctccactt cgtactccca aggcgaatca
     1141 tgcttatgtg cataaatttg ctgattcgca atcgatacgt acattcttac tttatcgttt
     1201 tccatcagac ccatcctttt ttgtcgtagt atggctcact ttggataaac gattcaatag
     1261 tcaaatggtg ttttcctctt cattcgcaac gcgttccact gcgctcgcca cattagcgtg
     1321 cactgctgga tccaatggat gtggaacgag atctcccggt tttgtactgt ccagaattgc
     1381 aagtgcagca gcaatgagca tcggataagt gatatctttt gcatgtgcat tcaatgcgcc
     1441 gcggaaaatt ccagggaatc caagaatatt gttcacttgt ctgccgtctg ctgcgaaagc
     1501 ggcgcccgct tccaatgccg cttccggctc gatttccgca ttgggattgg acagtgccaa
     1561 aatgatctgg ccttttctga ccatctccgg cttgatcaac ccttgtacac ctgtcgttgc
     1621 aatgacgata tcacaagttt tcatgatctc gtcgactgaa tccaaagccg ttccgccatg
     1681 ttgttccagt ctatcggccg cttcaggatt cgggtcgacc ccgtacattt cctttacgcc
     1741 atatgccatg aacatcctgc tgattgcgag gcccgcagca cctagaccga tttgaccgac
     1801 tacagattcc gacaattcca cgcctgattg acgacacgcc gaaagagctg aagctagcgt
     1861 gacgacagca gtgccatgtt ggtcgtcatg catgaccgga atatccagtt ccgctttcaa
     1921 cctttcttcg atttcaaagc aatgcggcga tccgatatcc tcgagaagaa tccctccgaa
     1981 actttggtgg atatgcttca cggtttccac gacctcatct ggattgctcg tgtctagtag
     2041 aataggaatc ccgctgatgc ctgcaaattg atcgaataag accgccttcc cttccatgac
     2101 aggcattcct gcaacaggtc caatatttcc aagccctaaa atggccgtac cgtccgtaac
     2161 gatggcaacc gtatttgaaa tccctgtaaa ataatcggcc tgcaccggat cgttttgaat
     2221 cactttacaa acattggcga ctcccggcgt gtaaattctt cggagatctc ctagtgaacg
     2281 gacctcaacc cggctcttca tcgaaatctt gccgccttca tgtgcatgca aaacatcatc
     2341 tgttacagca tggacatgaa tcccgtcacc gattgcatta atggcagcaa caatgctttc
     2401 caactgctct tcgcttgtac actgaattga aacatccctt atcgtcgata aagtcccgac
     2461 tttaatcgtt tggatatctc caatatcccc cttcaattca ccgatggccg atgcgacctt
     2521 cgcaaaattt ccaggtttcg atggcgtttc aataattaaa ttccgtataa attggatatg
     2581 tgacatgatg tcgcctccta tatgatccct ctaacttccc gtaccccatt ttacacataa
     2641 taattgacct caacaaaaat aaagcccgaa ttccctagat tgagaggaaa ttcgggctcc
     2701 ttgcttcagg ccttcacatc gacagccgaa cctttataag gatggactgc cggttgatcc
     2761 tgcatacttt gcagcatatc cgtcactgca tttgcattca ccgataatgc cttgttcatg
     2821 acactcaatt gaacagtgga ttgcaaactt ctcaattgac ttgccatgat ggagttcatg
     2881 ttcataaacc attcctcctt tacaccgcta ttatcggaat aaaaaccaat gattcaagac
     2941 ctggtcatca tttattgtct tcaccaagga atgcattcag catccattgg tgcttctcaa
     3001 tgctttgata cgtcgcattc aataagtcct ctgtcatatc atcgcccgct tcggcagcgt
     3061 actccatgcc ctttttcagt gacttcataa tgttaccgaa gtcatcagcg atcgttttca
     3121 ccatatcttc agcctgctcg tcgcctttcg cctctttaac gacggattgc tccagatgtt
     3181 cttttaatgt cgctgtcggg ttccctccga gcgtcaatac cctttcagca atctcatcca
     3241 tatgggcggt cgcctcatta taaagttctt caaattttgc atgcaaggtg aaaaattgct
     3301 tccctttcac ataccaatga taattatgca actttgcata catgaccgac catgtggata
     3361 cttgtacatt cagttcatcc atcaattcct tggacataaa aaaacccctc ttcatttttc
     3421 t
//
LOCUS       DASULI010000473         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Sporosarcina sp. isolate SMAG_U1540 k141_1903834,
            whole genome shotgun sequence.
ACCESSION   DASULI010000473 DASULI010000000
VERSION     DASULI010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961683
            Sequence Read Archive: SRR25158531
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sporosarcina sp. (soil metagenome)
  ORGANISM  Sporosarcina sp.
            Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae;
            Sporosarcina.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 07:41:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,042
            CDSs (total)                      :: 2,023
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 19
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 11
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Sporosarcina sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903834"
                     /isolate="SMAG_U1540"
                     /isolation_source="soil"
                     /db_xref="taxon:49982"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.46361111 N 121.3783333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFX34_10205"
     CDS             <1..819
                     /locus_tag="VFX34_10205"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009496785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5565326.1"
                     /translation="LIESEKAPSLLKKSMLYSVNAGGKRIRPLLVLAVLADFGKKTVD
                     ELKVACAVELIHTYSLIHDDLPCMDDDDFRRGKPTNHKVFGEATAVLAGDAMQTSAFG
                     ILATLEHARPDQVIRLVALLAEASGANGMVGGQILDMEGETSSLSLQELEEVHKHKTG
                     ALLSFCIEAGAILSEAQDAELEQLRKYAKNIGLAFQIKDDILDVTATTEQLGKTANSD
                     AASDKSTYPSLLGLEGAQQQLDRYHRDAVGSLAFLEKEDSMLKLFADYIVQRNA"
     gene            948..>2469
                     /gene="dxs"
                     /locus_tag="VFX34_10210"
     CDS             948..>2469
                     /gene="dxs"
                     /locus_tag="VFX34_10210"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5565327.1"
                     /translation="MDLTKISNPSFLKKLDKEEMTELAKEIRKFLIENLSVTGGHIGP
                     NLGVVELTLALHKLFDSPKDKLIWDVGHQAYVHKILTGRAGQFGTLRQYKGLSGFPKM
                     SESEHDVWETGHSSTSLSAALGMAAARDMQGGTNHVIPVIGDGALTGGMALEALNHIG
                     HLKTNMIVILNDNEMSIAPNVGALHDILGKLRTAGKYNSVKDELEYILKKIPAVGGKV
                     AAAAERVKDSLKYLLVSGVFFEELGFTYLGPIDGHDFNDLERNLQYAKKVEGPVLLHV
                     LTKKGKGYRPAENDKIGTWHGTGPYKIETGDFVKSPTTAPSWSGLVAETVRKIAREDK
                     RIAAITPAMPVGSKLESFAAEFPERFFDVGIAEQHATTMAAGLATQGMKPFLAIYSTF
                     LQRAYDQMLHDITRQKLNVFIGIDRAGLVGADGETHHGVYDISFLRHMPNLVIMMPKD
                     ENEGQHMVKTAIEYNDGPIAMRYPRGNGLGVVMDENLKTIPIGTWEVLREGRDGAIL"
BASE COUNT          704 a          512 c          659 g          594 t
ORIGIN      
        1 ctgatcgaat cggaaaaagc accatcttta ttaaagaaat caatgcttta ttccgtgaat
       61 gcaggcggca aacggatccg cccacttctt gtgctagcgg tgctagctga cttcggtaaa
      121 aaaactgtag atgaactgaa ggtcgcatgc gcagttgaac tcatccatac atactccctc
      181 atccacgatg acctgccatg catggacgat gatgatttca gaagaggaaa gccgactaat
      241 cataaagtat ttggagaagc gacggcagtt cttgcgggag atgcaatgca gacgagcgca
      301 ttcgggattt tggctacgct ggaacatgcc cgaccggacc aggttattag attggttgct
      361 ctgttagcgg aagcttccgg agcgaatggc atggttggag gccaaatcct ggatatggaa
      421 ggtgaaacgt cctccctgtc attgcaagag cttgaagagg tgcataagca taagacggga
      481 gccttattat cattctgtat cgaggcaggt gcaatccttt ccgaggcgca ggatgcggag
      541 ttggaacagc ttcgcaaata cgccaagaac atcgggctgg cattccagat caaggacgat
      601 attttggatg tcacggcaac gaccgaacag ctaggcaaga cagcgaatag cgatgctgcc
      661 agcgacaaat cgacgtatcc ttcacttttg ggactagaag gcgcacagca gcagctggat
      721 agatatcacc gcgatgcagt ggggagcctg gcctttttag agaaggaaga ctccatgtta
      781 aaacttttcg ccgattatat cgtccaaaga aatgcatgat attcctttgg tatgaaaaga
      841 gctttaccgg ataatggtcc aatagttcct attgtagagc cttcttgtta taatgaaaac
      901 attacttgga cttacgcggc aataaattat gaaggtgtgt gatagtagtg gatctcacta
      961 aaatttcaaa tccgtctttt ctaaaaaagc ttgataaaga agaaatgacg gaattggcaa
     1021 aagaaatccg gaaatttctc attgagaatt tatcggtgac aggtggtcat atcggtccga
     1081 acttaggtgt tgtcgaattg acgcttgctt tgcataaatt gttcgacagc ccaaaggata
     1141 aactaatttg ggatgttggg caccaagctt atgtgcataa gattttgaca gggcgagcag
     1201 gccaattcgg gacgctccgc caatacaagg gcttgagcgg cttcccgaaa atgtcggaaa
     1261 gcgagcatga tgtatgggag acgggacaca gctccacttc cctttccgcc gctttaggaa
     1321 tggctgctgc cagagacatg caaggcggta caaaccatgt cattccggtc atcggggacg
     1381 gtgcattgac aggcggcatg gctttggagg cattgaatca tatcggtcat ctgaagacga
     1441 acatgattgt cattttaaac gacaatgaaa tgtccattgc cccaaacgtc ggggcgctgc
     1501 acgatatcct cggcaagtta aggacagccg ggaaatacaa tagcgttaaa gatgagttgg
     1561 aatatatact gaaaaagata ccggcagtcg gtgggaaagt ggcggctgca gctgaacggg
     1621 tgaaggatag cttgaaatat ctgctcgttt ccggcgtgtt tttcgaagag ctcggcttca
     1681 cttatttggg gccgattgac ggtcatgatt tcaatgactt ggaaaggaat ctccaatacg
     1741 ccaagaaggt ggaagggccg gtccttctac atgtcctgac caaaaaaggg aaaggctatc
     1801 gaccggcgga aaatgacaaa atcggaactt ggcatggcac aggtccttat aagatcgaga
     1861 cgggagattt tgtgaaatca ccgaccactg ctccatcatg gagcgggctg gtagctgaaa
     1921 ctgtaaggaa aatcgccagg gaagacaagc gtattgctgc aattacacca gcaatgcctg
     1981 taggttcgaa attggagtca tttgcagctg aatttccgga acgtttcttt gatgtaggta
     2041 ttgctgaaca gcatgccaca acaatggctg caggcttggc aacacaaggg atgaaaccgt
     2101 ttttggcgat ctattcgaca ttccttcaac gggcatatga ccagatgctt catgatatta
     2161 cgagacagaa gctcaatgta tttatcggca tcgatcgtgc cgggctcgta ggtgctgacg
     2221 gagaaaccca tcatggtgtg tatgacattt catttttacg gcatatgccg aatcttgtca
     2281 tcatgatgcc gaaagatgaa aacgaagggc agcatatggt aaagactgct atcgagtata
     2341 atgatggccc aatcgccatg agatatccaa gaggaaacgg tctcggcgtc gtgatggacg
     2401 agaacctgaa aacgatacct atcggcactt gggaagtatt gcgtgaaggc agggacggtg
     2461 cgattttga
//
